LOCUS       DAOFMX010000001       136740 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.001, whole genome shotgun sequence.
ACCESSION   DAOFMX010000001 DAOFMX010000000
VERSION     DAOFMX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 136740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 136740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..136740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.001"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="QBA20_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="QBA20_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDQ6290708.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="QBA20_000002"
     CDS             complement(1672..2730)
                     /locus_tag="QBA20_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDQ6290709.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="QBA20_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="QBA20_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="QBA20_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="QBA20_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDQ6290710.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="QBA20_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="QBA20_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDQ6290711.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="QBA20_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="QBA20_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDQ6290712.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="QBA20_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="QBA20_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDQ6290713.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSRIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="QBA20_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="QBA20_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDQ6290714.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="QBA20_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="QBA20_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDQ6290715.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="QBA20_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="QBA20_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDQ6290716.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFISITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="QBA20_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="QBA20_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDQ6290717.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="QBA20_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="QBA20_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDQ6290718.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="QBA20_000013"
     CDS             complement(17699..18322)
                     /locus_tag="QBA20_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDQ6290719.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="QBA20_000014"
     CDS             18619..18894
                     /locus_tag="QBA20_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290720.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="QBA20_000015"
     CDS             complement(18971..19213)
                     /locus_tag="QBA20_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDQ6290721.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="QBA20_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="QBA20_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDQ6290722.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20840..20911
                     /gene="ypdK"
                     /locus_tag="QBA20_000017"
     CDS             20840..20911
                     /gene="ypdK"
                     /locus_tag="QBA20_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDQ6290723.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="QBA20_000018"
     CDS             complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="QBA20_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDQ6290724.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22591..24077
                     /locus_tag="QBA20_000019"
                     /pseudo
     CDS             22591..24077
                     /locus_tag="QBA20_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24092..24826
                     /gene="ypdB"
                     /locus_tag="QBA20_000020"
     CDS             24092..24826
                     /gene="ypdB"
                     /locus_tag="QBA20_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDQ6290725.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24839..25696
                     /locus_tag="QBA20_000021"
     CDS             24839..25696
                     /locus_tag="QBA20_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6290726.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25699..28194)
                     /gene="ptsP"
                     /locus_tag="QBA20_000022"
     CDS             complement(25699..28194)
                     /gene="ptsP"
                     /locus_tag="QBA20_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDQ6290727.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28219..29256)
                     /gene="ypdE"
                     /locus_tag="QBA20_000023"
     CDS             complement(28219..29256)
                     /gene="ypdE"
                     /locus_tag="QBA20_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDQ6290728.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29256..30339)
                     /gene="ypdF"
                     /locus_tag="QBA20_000024"
                     /pseudo
     CDS             complement(29256..30339)
                     /gene="ypdF"
                     /locus_tag="QBA20_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30354..31601)
                     /locus_tag="QBA20_000025"
     CDS             complement(30354..31601)
                     /locus_tag="QBA20_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDQ6290729.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31623..31949)
                     /locus_tag="QBA20_000026"
     CDS             complement(31623..31949)
                     /locus_tag="QBA20_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDQ6290730.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32168..33133)
                     /gene="glk"
                     /locus_tag="QBA20_000027"
     CDS             complement(32168..33133)
                     /gene="glk"
                     /locus_tag="QBA20_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDQ6290731.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33337..34593
                     /locus_tag="QBA20_000028"
     CDS             33337..34593
                     /locus_tag="QBA20_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDQ6290732.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPNPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34708..35034
                     /locus_tag="QBA20_000029"
     CDS             34708..35034
                     /locus_tag="QBA20_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDQ6290733.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35175..36413)
                     /locus_tag="QBA20_000030"
     CDS             complement(35175..36413)
                     /locus_tag="QBA20_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDQ6290734.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36749..37951
                     /gene="nupC"
                     /locus_tag="QBA20_000031"
     CDS             36749..37951
                     /gene="nupC"
                     /locus_tag="QBA20_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDQ6290735.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38001..39316)
                     /locus_tag="QBA20_000032"
                     /pseudo
     CDS             complement(38001..39316)
                     /locus_tag="QBA20_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163199.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(39456..40082)
                     /locus_tag="QBA20_000033"
     CDS             complement(39456..40082)
                     /locus_tag="QBA20_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HDQ6290736.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRIYLKLAGSVKRAATSVVHTL"
     gene            complement(40290..40365)
                     /locus_tag="QBA20_000034"
     tRNA            complement(40290..40365)
                     /locus_tag="QBA20_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40330..40332),aa:Ala,seq:ggc)
     gene            complement(40405..40480)
                     /locus_tag="QBA20_000035"
     tRNA            complement(40405..40480)
                     /locus_tag="QBA20_000035"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40445..40447),aa:Ala,seq:ggc)
     gene            40701..41060
                     /locus_tag="QBA20_000036"
     CDS             40701..41060
                     /locus_tag="QBA20_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDQ6290737.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41062..41454
                     /locus_tag="QBA20_000037"
     CDS             41062..41454
                     /locus_tag="QBA20_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDQ6290738.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41506..42921)
                     /gene="gltX"
                     /locus_tag="QBA20_000038"
     CDS             complement(41506..42921)
                     /gene="gltX"
                     /locus_tag="QBA20_000038"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDQ6290739.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43180..43255
                     /locus_tag="QBA20_000039"
     tRNA            43180..43255
                     /locus_tag="QBA20_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43213..43215,aa:Val,seq:tac)
     gene            43300..43375
                     /locus_tag="QBA20_000040"
     tRNA            43300..43375
                     /locus_tag="QBA20_000040"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43333..43335,aa:Val,seq:tac)
     gene            43380..43455
                     /locus_tag="QBA20_000041"
     tRNA            43380..43455
                     /locus_tag="QBA20_000041"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43413..43415,aa:Lys,seq:ttt)
     gene            43486..43611
                     /locus_tag="QBA20_000042"
     ncRNA           43486..43611
                     /ncRNA_class="other"
                     /locus_tag="QBA20_000042"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43731..44072
                     /locus_tag="QBA20_000043"
     CDS             43731..44072
                     /locus_tag="QBA20_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HDQ6290740.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44063..44989)
                     /locus_tag="QBA20_000044"
     CDS             complement(44063..44989)
                     /locus_tag="QBA20_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6290741.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45079..46077
                     /locus_tag="QBA20_000045"
     CDS             45079..46077
                     /locus_tag="QBA20_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDQ6290742.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46074..46292)
                     /locus_tag="QBA20_000046"
     CDS             complement(46074..46292)
                     /locus_tag="QBA20_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDQ6290743.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46294..48309)
                     /gene="ligA"
                     /locus_tag="QBA20_000047"
     CDS             complement(46294..48309)
                     /gene="ligA"
                     /locus_tag="QBA20_000047"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDQ6290744.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFVGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48380..49366)
                     /gene="zipA"
                     /locus_tag="QBA20_000048"
     CDS             complement(48380..49366)
                     /gene="zipA"
                     /locus_tag="QBA20_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDQ6290745.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49596..50357
                     /gene="cysZ"
                     /locus_tag="QBA20_000049"
     CDS             49596..50357
                     /gene="cysZ"
                     /locus_tag="QBA20_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDQ6290746.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50542..51513
                     /gene="cysK"
                     /locus_tag="QBA20_000050"
     CDS             50542..51513
                     /gene="cysK"
                     /locus_tag="QBA20_000050"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDQ6290747.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51897..52154
                     /gene="ptsH"
                     /locus_tag="QBA20_000051"
     CDS             51897..52154
                     /gene="ptsH"
                     /locus_tag="QBA20_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDQ6290748.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52199..53926
                     /gene="ptsI"
                     /locus_tag="QBA20_000052"
     CDS             52199..53926
                     /gene="ptsI"
                     /locus_tag="QBA20_000052"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDQ6290749.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53967..54476
                     /gene="crr"
                     /locus_tag="QBA20_000053"
     CDS             53967..54476
                     /gene="crr"
                     /locus_tag="QBA20_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDQ6290750.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54518..55369)
                     /gene="pdxK"
                     /locus_tag="QBA20_000054"
                     /gene_synonym="thiJ"
     CDS             complement(54518..55369)
                     /gene="pdxK"
                     /locus_tag="QBA20_000054"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDQ6290751.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55474..55848
                     /locus_tag="QBA20_000055"
     CDS             55474..55848
                     /locus_tag="QBA20_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDQ6290752.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55881..56604
                     /locus_tag="QBA20_000056"
                     /pseudo
     CDS             55881..56604
                     /locus_tag="QBA20_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56808..57719)
                     /gene="cysM"
                     /locus_tag="QBA20_000057"
     CDS             complement(56808..57719)
                     /gene="cysM"
                     /locus_tag="QBA20_000057"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDQ6290753.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSLGAGI"
     gene            complement(57853..58950)
                     /gene="cysA"
                     /locus_tag="QBA20_000058"
     CDS             complement(57853..58950)
                     /gene="cysA"
                     /locus_tag="QBA20_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDQ6290754.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58940..59815)
                     /gene="cysW"
                     /locus_tag="QBA20_000059"
     CDS             complement(58940..59815)
                     /gene="cysW"
                     /locus_tag="QBA20_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDQ6290755.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59815..60648)
                     /gene="cysT"
                     /locus_tag="QBA20_000060"
     CDS             complement(59815..60648)
                     /gene="cysT"
                     /locus_tag="QBA20_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDQ6290756.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVTLSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60648..61664)
                     /gene="cysP"
                     /locus_tag="QBA20_000061"
     CDS             complement(60648..61664)
                     /gene="cysP"
                     /locus_tag="QBA20_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDQ6290757.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61968..62759)
                     /gene="ucpA"
                     /locus_tag="QBA20_000062"
     CDS             complement(61968..62759)
                     /gene="ucpA"
                     /locus_tag="QBA20_000062"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDQ6290758.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62946..63746)
                     /gene="murR"
                     /locus_tag="QBA20_000063"
     CDS             complement(62946..63746)
                     /gene="murR"
                     /locus_tag="QBA20_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDQ6290759.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63910..64806
                     /gene="murQ"
                     /locus_tag="QBA20_000064"
     CDS             63910..64806
                     /gene="murQ"
                     /locus_tag="QBA20_000064"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDQ6290760.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64810..66234
                     /gene="murP"
                     /locus_tag="QBA20_000065"
                     /pseudo
     CDS             64810..66234
                     /gene="murP"
                     /locus_tag="QBA20_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(66414..67313)
                     /gene="yfeX"
                     /locus_tag="QBA20_000066"
     CDS             complement(66414..67313)
                     /gene="yfeX"
                     /locus_tag="QBA20_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDQ6290761.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67409..67984)
                     /locus_tag="QBA20_000067"
     CDS             complement(67409..67984)
                     /locus_tag="QBA20_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDQ6290762.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68045..68494)
                     /locus_tag="QBA20_000068"
     CDS             complement(68045..68494)
                     /locus_tag="QBA20_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDQ6290763.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68481..68906)
                     /locus_tag="QBA20_000069"
     CDS             complement(68481..68906)
                     /locus_tag="QBA20_000069"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDQ6290764.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69120..69989
                     /gene="amiA"
                     /locus_tag="QBA20_000070"
     CDS             69120..69989
                     /gene="amiA"
                     /locus_tag="QBA20_000070"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDQ6290765.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69993..70892
                     /gene="hemF"
                     /locus_tag="QBA20_000071"
     CDS             69993..70892
                     /gene="hemF"
                     /locus_tag="QBA20_000071"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDQ6290766.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70898..71950)
                     /gene="eutR"
                     /locus_tag="QBA20_000072"
                     /pseudo
     CDS             complement(70898..71950)
                     /gene="eutR"
                     /locus_tag="QBA20_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(71996..72496)
                     /gene="eutK"
                     /locus_tag="QBA20_000073"
     CDS             complement(71996..72496)
                     /gene="eutK"
                     /locus_tag="QBA20_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDQ6290767.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72509..73168)
                     /gene="eutL"
                     /locus_tag="QBA20_000074"
     CDS             complement(72509..73168)
                     /gene="eutL"
                     /locus_tag="QBA20_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDQ6290768.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74350..75141
                     /locus_tag="QBA20_000075"
     CDS             74350..75141
                     /locus_tag="QBA20_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290769.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75861..76256
                     /locus_tag="QBA20_000076"
     CDS             75861..76256
                     /locus_tag="QBA20_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDQ6290770.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76274..76537
                     /locus_tag="QBA20_000077"
     CDS             76274..76537
                     /locus_tag="QBA20_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290771.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76560..77102
                     /locus_tag="QBA20_000078"
     CDS             76560..77102
                     /locus_tag="QBA20_000078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290772.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77241..77528
                     /locus_tag="QBA20_000079"
     CDS             77241..77528
                     /locus_tag="QBA20_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290773.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77567..77773
                     /locus_tag="QBA20_000080"
     CDS             77567..77773
                     /locus_tag="QBA20_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290774.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78085..78570
                     /locus_tag="QBA20_000081"
     CDS             78085..78570
                     /locus_tag="QBA20_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDQ6290775.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78551..78829
                     /locus_tag="QBA20_000082"
     CDS             78551..78829
                     /locus_tag="QBA20_000082"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290776.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78822..80282
                     /locus_tag="QBA20_000083"
     CDS             78822..80282
                     /locus_tag="QBA20_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ6290777.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80316..81125
                     /gene="murI"
                     /locus_tag="QBA20_000084"
     CDS             80316..81125
                     /gene="murI"
                     /locus_tag="QBA20_000084"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDQ6290778.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81244..81938)
                     /locus_tag="QBA20_000085"
     CDS             complement(join(81244..81692,81692..81938))
                     /locus_tag="QBA20_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDQ6290779.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82457..83830)
                     /gene="eutA"
                     /locus_tag="QBA20_000086"
     CDS             complement(82457..83830)
                     /gene="eutA"
                     /locus_tag="QBA20_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDQ6290780.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83805..84284)
                     /gene="eutP"
                     /locus_tag="QBA20_000087"
     CDS             complement(83805..84284)
                     /gene="eutP"
                     /locus_tag="QBA20_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDQ6290781.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84297..84632)
                     /gene="eutS"
                     /locus_tag="QBA20_000088"
     CDS             complement(84297..84632)
                     /gene="eutS"
                     /locus_tag="QBA20_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDQ6290782.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84925..87204)
                     /gene="maeB"
                     /locus_tag="QBA20_000089"
     CDS             complement(84925..87204)
                     /gene="maeB"
                     /locus_tag="QBA20_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDQ6290783.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            87493..88443
                     /gene="tal"
                     /locus_tag="QBA20_000090"
     CDS             87493..88443
                     /gene="tal"
                     /locus_tag="QBA20_000090"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDQ6290784.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            88463..90466
                     /gene="tkt"
                     /locus_tag="QBA20_000091"
     CDS             88463..90466
                     /gene="tkt"
                     /locus_tag="QBA20_000091"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDQ6290785.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKKAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90569..91612)
                     /locus_tag="QBA20_000092"
     CDS             complement(90569..91612)
                     /locus_tag="QBA20_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDQ6290786.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91738..92313)
                     /gene="nudK"
                     /locus_tag="QBA20_000093"
     CDS             complement(91738..92313)
                     /gene="nudK"
                     /locus_tag="QBA20_000093"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDQ6290787.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(92381..94360)
                     /gene="aegA"
                     /locus_tag="QBA20_000094"
     CDS             complement(92381..94360)
                     /gene="aegA"
                     /locus_tag="QBA20_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDQ6290788.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            94566..96283
                     /gene="narQ"
                     /locus_tag="QBA20_000095"
                     /pseudo
     CDS             94566..96283
                     /gene="narQ"
                     /locus_tag="QBA20_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            96485..99598
                     /gene="acrD"
                     /locus_tag="QBA20_000096"
     CDS             96485..99598
                     /gene="acrD"
                     /locus_tag="QBA20_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDQ6290789.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(99697..99756)
                     /gene="ypfM"
                     /locus_tag="QBA20_000097"
     CDS             complement(99697..99756)
                     /gene="ypfM"
                     /locus_tag="QBA20_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDQ6290790.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            100137..100493
                     /locus_tag="QBA20_000098"
     CDS             100137..100493
                     /locus_tag="QBA20_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDQ6290791.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            100497..101624
                     /gene="dapE"
                     /locus_tag="QBA20_000099"
     CDS             100497..101624
                     /gene="dapE"
                     /locus_tag="QBA20_000099"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDQ6290792.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            101652..101852
                     /locus_tag="QBA20_000100"
     CDS             101652..101852
                     /locus_tag="QBA20_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDQ6290793.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(101962..102660)
                     /gene="ypfH"
                     /locus_tag="QBA20_000101"
     CDS             complement(101962..102660)
                     /gene="ypfH"
                     /locus_tag="QBA20_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDQ6290794.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(102734..104749)
                     /gene="tmcA"
                     /locus_tag="QBA20_000102"
     CDS             complement(102734..104749)
                     /gene="tmcA"
                     /locus_tag="QBA20_000102"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDQ6290795.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(104764..105627)
                     /locus_tag="QBA20_000103"
     CDS             complement(104764..105627)
                     /locus_tag="QBA20_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDQ6290796.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(105795..106508)
                     /gene="purC"
                     /locus_tag="QBA20_000104"
     CDS             complement(105795..106508)
                     /gene="purC"
                     /locus_tag="QBA20_000104"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDQ6290797.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(106721..107755)
                     /gene="bamC"
                     /locus_tag="QBA20_000105"
     CDS             complement(106721..107755)
                     /gene="bamC"
                     /locus_tag="QBA20_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDQ6290798.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGLFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(107772..108650)
                     /gene="dapA"
                     /locus_tag="QBA20_000106"
     CDS             complement(107772..108650)
                     /gene="dapA"
                     /locus_tag="QBA20_000106"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDQ6290799.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            108796..109368
                     /locus_tag="QBA20_000107"
     CDS             108796..109368
                     /locus_tag="QBA20_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDQ6290800.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            109368..109838
                     /gene="bcp"
                     /locus_tag="QBA20_000108"
     CDS             109368..109838
                     /gene="bcp"
                     /locus_tag="QBA20_000108"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDQ6290801.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            110091..110708
                     /gene="hyfA"
                     /locus_tag="QBA20_000109"
     CDS             110091..110708
                     /gene="hyfA"
                     /locus_tag="QBA20_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDQ6290802.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            110708..112725
                     /gene="hyfB"
                     /locus_tag="QBA20_000110"
                     /pseudo
     CDS             110708..112725
                     /gene="hyfB"
                     /locus_tag="QBA20_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            112736..113683
                     /gene="hyfC"
                     /locus_tag="QBA20_000111"
     CDS             112736..113683
                     /gene="hyfC"
                     /locus_tag="QBA20_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDQ6290803.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            113700..115139
                     /gene="hyfD"
                     /locus_tag="QBA20_000112"
     CDS             113700..115139
                     /gene="hyfD"
                     /locus_tag="QBA20_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HDQ6290804.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            115151..115801
                     /gene="hyfE"
                     /locus_tag="QBA20_000113"
     CDS             115151..115801
                     /gene="hyfE"
                     /locus_tag="QBA20_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDQ6290805.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            115806..117392
                     /gene="hyfF"
                     /locus_tag="QBA20_000114"
     CDS             115806..117392
                     /gene="hyfF"
                     /locus_tag="QBA20_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HDQ6290806.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICIVGVAFGLFGTVLVYANAASVMPQAEMAISWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            117382..119096
                     /gene="hyfG"
                     /locus_tag="QBA20_000115"
                     /pseudo
     CDS             117382..119096
                     /gene="hyfG"
                     /locus_tag="QBA20_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            119106..119651
                     /gene="hyfH"
                     /locus_tag="QBA20_000116"
     CDS             119106..119651
                     /gene="hyfH"
                     /locus_tag="QBA20_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDQ6290807.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            119648..120406
                     /gene="hyfI"
                     /locus_tag="QBA20_000117"
     CDS             119648..120406
                     /gene="hyfI"
                     /locus_tag="QBA20_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HDQ6290808.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDLRLNSIVQQLFRVLRGLHD"
     gene            120399..120812
                     /gene="hyfJ"
                     /locus_tag="QBA20_000118"
     CDS             120399..120812
                     /gene="hyfJ"
                     /locus_tag="QBA20_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDQ6290809.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            120842..122854
                     /gene="hyfR"
                     /locus_tag="QBA20_000119"
     CDS             120842..122854
                     /gene="hyfR"
                     /locus_tag="QBA20_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDQ6290810.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            122876..123724
                     /locus_tag="QBA20_000120"
                     /pseudo
     CDS             122876..123724
                     /locus_tag="QBA20_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(123762..124823)
                     /locus_tag="QBA20_000121"
     CDS             complement(123762..124823)
                     /locus_tag="QBA20_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDQ6290811.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            125036..126499
                     /gene="bepA"
                     /locus_tag="QBA20_000122"
     CDS             125036..126499
                     /gene="bepA"
                     /locus_tag="QBA20_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDQ6290812.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            126520..126879
                     /gene="arsC"
                     /locus_tag="QBA20_000123"
     CDS             126520..126879
                     /gene="arsC"
                     /locus_tag="QBA20_000123"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDQ6290813.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(127012..127758)
                     /gene="hda"
                     /locus_tag="QBA20_000124"
     CDS             complement(127012..127758)
                     /gene="hda"
                     /locus_tag="QBA20_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDQ6290814.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(127808..129097)
                     /gene="uraA"
                     /locus_tag="QBA20_000125"
     CDS             complement(127808..129097)
                     /gene="uraA"
                     /locus_tag="QBA20_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDQ6290815.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(129183..129809)
                     /gene="upp"
                     /locus_tag="QBA20_000126"
     CDS             complement(129183..129809)
                     /gene="upp"
                     /locus_tag="QBA20_000126"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDQ6290816.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            130134..131171
                     /gene="purM"
                     /locus_tag="QBA20_000127"
     CDS             130134..131171
                     /gene="purM"
                     /locus_tag="QBA20_000127"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDQ6290817.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            131171..131809
                     /gene="purN"
                     /locus_tag="QBA20_000128"
     CDS             131171..131809
                     /gene="purN"
                     /locus_tag="QBA20_000128"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDQ6290818.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            131981..134047
                     /gene="ppk1"
                     /locus_tag="QBA20_000129"
     CDS             131981..134047
                     /gene="ppk1"
                     /locus_tag="QBA20_000129"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDQ6290819.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            134052..135593
                     /gene="ppx"
                     /locus_tag="QBA20_000130"
     CDS             134052..135593
                     /gene="ppx"
                     /locus_tag="QBA20_000130"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDQ6290820.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(135632..>136740)
                     /gene="pdeF"
                     /locus_tag="QBA20_000131"
     CDS             complement(135632..>136740)
                     /gene="pdeF"
                     /locus_tag="QBA20_000131"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDQ6290821.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        34141 a        35174 c        33598 g        33827 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taatcccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcagaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaagaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaataa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccatgataac atcatgattt ttatagcttt ttcttaagtt ttttccttcg ctgaatatct
    20581 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag
    20641 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga
    20701 gcagtttagc tcagaattct ggggtgatgc ccacggtaaa atacagggct tttaaggtcg
    20761 ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
    20821 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggtcatcgt
    20881 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
    20941 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
    21001 ttgctgctga cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc
    21061 tgacgaatac ggtcgtggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg
    21121 ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt cttggcaaat
    21181 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
    21241 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg
    21301 cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc
    21361 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
    21421 gcactgacca gcgttttgtt gccgaccata aagccgatac gccagcccgc catgttgtag
    21481 cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt
    21541 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
    21601 acatcgtagc atttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc
    21661 gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
    21721 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
    21781 tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc
    21841 accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
    21901 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
    21961 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
    22021 gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
    22081 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
    22141 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
    22201 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
    22261 gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
    22321 aacattttta tcaatggatt taaatgagga actccacttc aaaaacagga aatttttcta
    22381 cgctaacaag aaaatcacca ccaattttat taacattata atttcaataa atttaccagc
    22441 cagaagtgca ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt
    22501 aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca
    22561 tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg
    22621 gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg
    22681 tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc
    22741 attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg
    22801 gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc
    22861 atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg
    22921 gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg
    22981 aaaatcccca aagcacagcg ctggcgctgg atatcgccaa caaaacgcta ccgctgtttc
    23041 gccatgtcaa tagcgagtca ttacgcaagg tctgcgaaat tatccgcgat gacattcacg
    23101 ccgatgcggt ggcgattact aataccgatc atgtgctggc ctatgttggc gtgggtgaac
    23161 ataactatca gaatggcgat gacttcatta gcccgactac ccgtcaggcg atgaattacg
    23221 gaaaaatcat cattaaaaac aatgatgaag cccaccgcac accagagatt cattccatgc
    23281 tggtgatccc attgtgggag aaaggggtcg tgaccggaac gctgaaaatt tactactgcc
    23341 acgcgcatca gatcacctcg tcattacagg aaatggcggt cggtctgtcg caaatcatct
    23401 ccacgcaact ggaggtttca cgcgccgagc agctacgtga aatggcaaat aaggcagagc
    23461 ttcgcgccct gcaaagcaaa attaatcccc attttctgtt taacgctctg aacgctattt
    23521 catcgtcaat ccgtctgaat ccggataccg ctcgccagtt gatctttaat ctgtcgcgtt
    23581 atctgcgcta taacattgaa ttaaaagacg atgagcaaat cgatatcaaa aaagagctgt
    23641 atcaaattaa agactatatt gccattgagc aggcccgctt tggcgacaag ctgacggtta
    23701 tctatgatat tgatgaagag gtgaattgct gcattcccag cctgctgatc cagccgttgg
    23761 tggagaacgc cattgtccac ggtattcagc cttgcaaagg taaaggcgtt gtcaccatca
    23821 gcgttgcaga gtgcggaaat cgggtacgca ttgcggtgcg agataccggg cacggcatcg
    23881 ttccaaaggt gattgagcgg gtcgaagcga atgaaatgcc gggcaataaa attggcctgc
    23941 tgaatgtcca tcatcgcgtg aagttattgt atggcgaggg gctgcatatc cgccgcctgg
    24001 agccggggac ggaaattgcg ttttacattc ctaaccaacg caccccagtc gcctcacagg
    24061 ctacgttatt gctttgagcc ggagtgatat tgtgaaagtc atcattgttg aagacgaatt
    24121 cctggcacaa caggaactga gctggctaat taaagagcac agccagatgg agattgtcgg
    24181 cacctttgac gacggtctgg acgtgttgaa gtttttgcag cataaccgcg tcgacgccat
    24241 ttttctggat atcaatattc cgtcgctgga tggcgtgttg ctggcgcaaa acatcagcca
    24301 gttcgcccat aaaccgttta ttgtgttcat caccgcgtgg aaagaacatg cggtagaagc
    24361 gtttgaactg gaggcgtttg actacattct caaaccgtat caggagtcac gtattaccgg
    24421 gatgctgcaa aaactggaag cggcctggca acaacagcag accagcagta cgcctgccgc
    24481 gacggtaacg cgtgagaatg acaccattaa tctggtgaaa gatgagcgaa taatcgtcac
    24541 gccaattaac gatatctatt acgccgaagc gcacgagaaa atgacctttg tctatacgcg
    24601 gcgtgaatcc tacgtaatgc cgatgaacat taccgaattt tgcagcaaac tgccgccgtc
    24661 gcattttttc cgctgccatc gctcattttg tgtcaatctg aacaaaatac gcgaaatcga
    24721 accgtggttt aataacacct acattctgcg actgaaagat ctggattttg aagtgccggt
    24781 cagccgcagc aaagtgaaag aatttcgcca gttaatgcat ctttaatcgg gaactttcat
    24841 gaaagcacca ggcttgcccg ccgatcaaca atttttcgcc gatctgttca gcggcctagt
    24901 gcttaacccg caactactgg ggcgcgtctg gtttgccagc cagcctgcct cgttaccggt
    24961 gggcagttta tgtattgatt ttccccgtct ggatatcgtg ctgcgcggcg aatacggcaa
    25021 cctgctggaa gcaaagcagc agcgaatggt ggaaggagaa atgctgttta ttccggcacg
    25081 cgcggctaat ttaccgatca acaacaaacc ggtgatgctg ttaagcctgg tgttcgcacc
    25141 gacctggctt gggttatcgt tttacgatag ccgcaccacg tcgttgttgc atcctgctcg
    25201 ccagacccag cttcccagcc tgcaacgcgg tgaaggtgaa gcgatgctta ccgccctcac
    25261 ccatcttagc cgttcgccgc tggagcagaa tatcattcag ccactggtgt taagtttgct
    25321 gcatctttgc cgcaacgtgg tgaatatgcc gccgggtaat tcacaaccgc gcggcgattt
    25381 tctctatcac agcatttgta actgggttca ggataattat gcccagccgc tcacccgcga
    25441 gagcgtggcg cagtttttta atatcacgcc caatcatctg tcaaaactgt ttgctcagca
    25501 tggaacgatg cgttttatcg agtatgtgcg ttgggtgcga atggcgaagg cgaggatgat
    25561 tttgcagaaa tatcatctgt caattcatga agtggcacag cgttgcggtt ttccggatag
    25621 cgactatttt tgtcgcgttt tccggcgtca gtttagtctg acgccgggag agtacagcgc
    25681 ccgttttcag ggctaacgtc agaaggttaa ttctgtttcc agcagcgtca ggatactttg
    25741 tgcatcttgc gcggcaaaca acgactggcg gaagttttta ttcaccagtt tacgcgccag
    25801 ctgcgagaag actttcacat gattcatccc ttcgttagca cccagcgtca gcatgatcac
    25861 cagttcgact tcgcccattt ctgactgcca gtcaaccggt ttcgccagcc gggcaatgct
    25921 gatactggaa tgacggatcc actgagattt ggtgtgcggg atcgctacgc caaaaccaac
    25981 gccggtggta acaatctctt cccgctgcca gacatcttct tccagctcaa acggatgttc
    26041 agtgcgcccg ttaacgccga ggttgccgca caggaactgg atcgcctgct ctttattgct
    26101 aaaatcctga tcaacaaaga tattctccag cgccagcagt gggcgaacgt cttcttccgg
    26161 cgtaaaggcg gtgagtaacg cttcaatttc ctgcgcactg cggcattcac atgcctgacg
    26221 cgccagttcc cgacacgcct cgctatccag ttgacgaagc tggcttttca ccgccggaat
    26281 acgcgggcta ctcatactca gctcatccag gcccagccca agcagtagcg gcagataacg
    26341 gctttcaccg cccagttcac cgcaaatgcc tacccatttg ccccgctgat gcgcagtggt
    26401 aactatttgc tgcaacatgc gcaggaacga tggcgtaatc gggttatata gcggcgatac
    26461 gcgcgggtta ttacgatcga ccgcatacag atactgggtc atatcgttgg agccgatact
    26521 gaagaaatcg acctcatcgc agaagtggtc gatgatgtag cacaccgacg gcacttccac
    26581 catgatccca agcgtaatcg tctctgcatg acgcaggcca tcgcgcttaa gctcaacgat
    26641 cgctttttgg atctcgcctt tcacccataa gatctgatcg aggctgtgaa ccatcgggat
    26701 catcaactgg gcgttgccga aactggcggc gcgcaaaatg gcccgcagtt gagtgcggaa
    26761 caggccagca aattccgggt aaatacgtac cgcgcgatag ccgaggaacg ggttctcttc
    26821 ctggggaata ttcagataag gaatgctttt atcgccgccg atgtccatcg tgcggaagat
    26881 aatcggcttg tcgcccgccg ccagtagcac ctgctggtag gcttcaaatt gctcctgctc
    26941 gtccggcgcg ctgtcgcgat ccatatagag catttcggta cggaacagcc cgacaccttc
    27001 cgcgccgttg gcaaacgcgc ctggcgcttc cagagcggtg ccgatattcg ccgcaatatc
    27061 aatacgcttg ttatcacggg aataggccag ctgcgcggca gcctgcgcct gttgtttttg
    27121 gcgtttatcc gccagcgtct gcgcgacctg ataataaccg ctcaccgcgt cattcgggtt
    27181 aatcgccagc acgccgcact gggcgtcaag cactgcaggt tgcccggcat aacgggcaat
    27241 cgcatccagc ggcaagccac tcagtaccgg gatcgccgag gcacgggcca gaatcagtgt
    27301 atgcgaggtg cgcccggttt tctccagaat catacccgca agatttttca aatcaaggct
    27361 caaaaactgg cttggggtta aatcttcagc caccagaatg gtcggttttt ccagcaccag
    27421 attgttgcgc ggcttcagtt ccggccaggt gatatgcagc aactgttcgc tgatatcgcg
    27481 aatgtcgctg acccgctcgc gcagataatc gctggcagaa gcagatagtt tggcgcaaac
    27541 ctgctccata ttgctgatga tcgccgcccc cagtccctga tgctgttctg tcatcaggcg
    27601 acggatattg cctgcaaatt catcatcctg aatcagcgac aaatgagcgc tgaggatagt
    27661 tttgctttcg ccgtcacgct cacgcaattg ctgattcagt tgctcggcaa gcgttgccag
    27721 gctgtgctcc agccgggtgg aatcttgcgc actggcgggg attgcccgat aactgtcgag
    27781 gctgtcgctt tgtaacaggg tcagcgtacc cacgccgacg ccgcttgcca gcacattgcc
    27841 gtacagtaaa tccgggttaa ggcggcttaa tgaacgcggc agcggatgcg ccgtcagttc
    27901 tgcctgcgtc ggctgaacgc tgtcgctgtc gataaagcgc accaggatgt actcttccag
    27961 cacgcgccgc gcctgctctt catcgctgcc gctaatgttc aggctgcaac tgtcattaaa
    28021 taaggtgcca gttccaatca acgccagcga gcttttagca tcagcctttg cgttctggcg
    28081 atggttgata aaggtgattt cgctttgcca ctggctgcac tgttctttaa gttcccacgc
    28141 cggacgagca tgtagaccgt taggcaaagg acagagaaat tgaatcgtta acataaccgc
    28201 tccgggttaa tcaggagatc atctgaaatc cgtcagttga acaaccgtct cacgcgtaag
    28261 acgttgaata agggcagata aaagttgctc catctgcaaa atgtcgcggc aatcggcaat
    28321 cgatgccgcg caatgtccgt ggcgggtggc aggccccatt accaatgtgg gtacgccagt
    28381 accggttaag tgcaccgctc caccgtccgt gccgccgtta ctgaacatat ccgcctgcaa
    28441 cggaacgcca atttctgccg ccacggtttc aatccacgcg gtaagttttg gcggcgcaat
    28501 cagcgattta tcgcttaaca ccagcatcgg cccgttacca atctgacgat ggttagccgc
    28561 gccataatca aagtttttcg cccagcaggc ggtatcaagc acaatggcga cgtccggcga
    28621 caccgcgcgg gtagcagttt gcccgccgcg taatcccacc tcttcgctgg aacttgccac
    28681 cagccacact tccgcaggca gttcggcgtc gtgcagctca cgtagtaacg tcaccagcag
    28741 atagcaactg aggcgatcat caaaggcttt gcccatcacc cgctggtgag ggaggacctg
    28801 aaaagtggta tcgaaggtga cgcgatcgcc tggacgaatt cccgcctgca tcacttcgtc
    28861 ataggtgcgc gcgccaatgt ccacgcgcat ggcgctaacg tcattcccct gccggtcgcc
    28921 gtcaagcagg cctggaattt tgcactcttc acgggtggtg atgcgcaccg gctgcagctg
    28981 gcgggcagcc atgcgcacgt tgccaaccgg cagcacgtca atagcgcctt cgcgggagat
    29041 gctgcgcacc ataaatccca cttcgtccat atgggcgcag atcatcacct tcggacctgt
    29101 cgattcattg aggcggatca gcaccgatcc cagaccatca aatcgcactt ctttttgcag
    29161 gcgagccgct tcttccagca ggatctgccg cacttcctgt tccgaggagg cgatcgcatc
    29221 tgcctcgctc aacgctttta atagcgataa atccattatg cctctcccgt gagcaacact
    29281 gttttcggca tggcgtagag cacttctgcg ccttgcgggg taaccagcac aacatcttcg
    29341 atgcgcacgc ccccttgccc tagcaaataa atccccggct ccacggtcag caacatgcct
    29401 ggctgtagcg tcgtggtgtc ccgcggtgaa aaacgcggat cttcatggac ttcaatgccg
    29461 atagcgtgac cagtgttatg accgaaatag tcgccaaatc cagcctcggt aatgacccgg
    29521 cgcgcggcgt cgtcaatctg ctggcagcgc acgccggggc gaattgcaga gattgccgcg
    29581 agctgtgcct gcaggacaat ctgatagacg tcaaacagcg ggtgagattc ggcgctcacc
    29641 ccttcgccat tcaccagcaa ggtgcgcgtc atatcagcag tagccctgat acagcgcacc
    29701 aaaatcgaga gtgacaaact cgcccgctgc aacaatcttg tcgctggctt tgccgtgcgg
    29761 cagcgccccg cgccagccgc tggcgacaat ggtgtcaaaa gaggcttttt ctgcgccctg
    29821 ctggcgcata aaccactcca gttcagcggc tatctcgcgc tcgctcatcc ccgcctgaat
    29881 aaagcggcga atatgctccg ctccgcgatc ggcaatccca caggcgaggc ggattatctc
    29941 cacctcctct ggcgttttga tttgccgcag cacatccggc gtggcgctaa ccagtttcgc
    30001 attgagttca gacttccagc ggtgcgcggt ttcccaactc acctgctggc cttcaaaacc
    30061 gagcgtttgc aactgctcat cggcaatgat ttgattgacg atagtggtaa gcgtgttcgt
    30121 cgcgtcaagc aaatgcagct ggtagccttg cgcacgggct tccacctcaa cgaaatagcg
    30181 cgaatctacc agaatgtgcg cactttcacg gctaataacc acatagcctg atccggtgga
    30241 gatccccaga tgcggctgtt tgttctgccg tgaggagaga agcaccgcat ccagttgttg
    30301 caccttaagc cagttgcgca gcgaagcgag taatgtcatc tggattttcc ttcttacagg
    30361 ctatcgatta acaatttgcc tttgcggaac atcatcagac gcaggaacac caccatcagc
    30421 gcagtaatga ttgcccccag cgcgatcccc gccatataaa cgcccaggtt agtgaccagc
    30481 ggccatgccc agatagcgga ttccgggaac cattgcactg cgcccagcca gacagctgcg
    30541 gttgagccga caatcgcgcc gaccatatat gacggaatag cggtgatggg gctttccagc
    30601 gcaaacggaa tcgcaccctc actaatcccc ataaaggcca ggaacatcgc ggtcttaccc
    30661 tgcggataaa gttgagcgtt gaacaggcgt ttgccggtta aacgacggtc aataatggtc
    30721 gccagaccca gaccaatcgg cggaataacg atagcgatag aacgagcggt gaccggtaat
    30781 acgtggtcag tggtaaagct gaaggcaacg aaacctgcgg ctttgttgat cgggccacca
    30841 aggtcgattg ctgtcgccgc ggcgatcccc attgcgtaca tcagcgcgcc cttctcacct
    30901 gcggcagtca gcacagtacg gataccgccg ttgatccagc caccaaaggg agtgatgacg
    30961 tagtacatcg ccagcatgac aaaaatggcg gaaagaatcg gtagcaaaaa tgtggttttg
    31021 aacgctaaca ggaaatccgg taactggatt ttctggttca tccatttcac aaggtaaccg
    31081 gcaacgatag aaataatcag cgcaccaatg aaggtggacg gcaccggcga agaggtcgcc
    31141 cattgcatag tgctgggatc gaagttcagc agttgcgtcg gctgggtaga catcaacccg
    31201 ccgataaaac cagccgggaa cgccagtttg ccaccgatag agttcgccac aaatgcagca
    31261 aacatcggaa tggcaaaacc aaacaacacg ccgccgaagg actgcgacag ccaggcaaat
    31321 ttaagtaagg agaggtcgaa accggagaat ttcccgctat taagagcatc catgatgccg
    31381 atctcagcag gaattttaag ccagctataa gcaatcagct ggctgaaagc gaggatcaca
    31441 ccgcccataa ttaaggtcgg caccatgcgt gaaatccctg acatcacatg ctgcgggagt
    31501 tcaccccaga agctgctttt agaggcctgc ggattcttaa ctgccgctgc cgcaccggat
    31561 gcgccaggca caacggttgc actgcgtttt ttaatggcca taacaattat ccctgtcgaa
    31621 tattactgtt gttcagaggc aatcatctct tcgatttctt tgatgatgcc cgcagcgttt
    31681 ttaattgcgt cctgcaaagt gatttcataa acgtcgcgtg attcgaaacg ttcgttatct
    31741 tccggggtaa ctgccacgga atggatgatg atggtcgctt cggcgatatc ctgcgccgtc
    31801 aggcgattct ggatgccgtc cgcgccctgg gtttcaattt tcacttcata accggcttct
    31861 accgccgctt cttccagcac ctgagcggcc ataaaagtgt gagctaggcc cattgggcag
    31921 gcacataagg caatcagttt cttactcatc gttaattcct cgttcagtga tagttgacga
    31981 gtgaaattat gaacgtgcaa aacaaatcgc cgttaccaga caaataatgc atttactgga
    32041 gaattaatcc cgctgtacgg aagtgtgacg caggtcggcc ttgtgagggg acacaaagaa
    32101 aaattgaatt aactgtatga tttaaaagat tatcgggaga gttacctccc gatataacag
    32161 gaaggattta cagaatgtga cctaaggtct ggcgtaaatg tgcaccggaa ccgagaagac
    32221 ccggatagtc atggacgatg agatacaccg gaatatcatg gacatattct ttaaagcgcc
    32281 ctttatcttc aaatgcggca cggaaaccgg aggctttgaa gaactcaagg aagcgcggca
    32341 cgataccgcc cgcaataaac acgccgccaa atgtcccgag attgagcgcc agattgccgc
    32401 caaaacggcc cataatgacg caaaacagcg acaatgcgcg gcggcaatcg gtgcagctgt
    32461 cagccagcgc gcgttcggta atatcttttg gcttgagatt ttctggcagg cggttgtcag
    32521 ctttcacaat tgcgcgatac aaattcacca gcccagggcc agaaagcacg cgctccgccg
    32581 aaacatgacc aatttccgca cgcaatattt cgaggataat ggcctcttct tcactattcg
    32641 gcgcaaaatc aacgtgaccg ccttcgcccg gcaagcttac ccaacgctta tcgacatgga
    32701 ccagatgcgc aaccccaagc cccgttccgg caccgtaaac cgcaataggc ttaccttcga
    32761 ccggttctgc gccaccaaac tgaatcagat gctctttttt cagcatcggg atcgccatcg
    32821 atacagcggt aaaatcgtta ataatttcca gatggctaaa accgagattc tttttcattt
    32881 cggcaattga gaacgcccag gtatggttgg tcattgccac ccagtcaccg gtaattgggc
    32941 aagcgatggc aatacagccg tctttcacct cgaccttatg ttcttcaaga taaacgcgaa
    33001 tgaccgcttc gaggctgggg taatcaagcc ctgaataggt cttagcctgc gagatttcac
    33061 cactggcaat atcacacaga gcaagacgtg cgttggtgcc gcccacatca ccgactaatg
    33121 catactttgt cattcttcaa ctgctccgct aaagtcaaaa taattctttc tcacactgta
    33181 aatacctggg ggcataacaa caacgccgga aaggcaggct ccctgtaaat atcgatctgg
    33241 gtcacacaat tactttatcg tttcagcacc aattgcagcg atgccttttt gcaagctggg
    33301 caaactaagt atctgacccc gcataaggaa tagaacatgc tccatccgcg agccagaacc
    33361 atgttgttat tatcgctccc cgccgtggca attgggattg cgtccagtct tattctgatt
    33421 gtggtgatga aaatcgcctc ggtattacag aatttgctct ggcaacgact gccgggaact
    33481 ctggggatag cccaggattc acccctctgg atcatcggtg tattaacgct aacgggtatt
    33541 gcggtggggt tggttatccg tttcagccag ggtcatgccg gaccaaaccc cgcctgtgaa
    33601 ccgctgatcg gcgcaccggt tccgccctct gcgctacctg gacttatcgt agcattaatt
    33661 ctcggtcttg ctggcggcgt cagcctgggg ccggaacatc cgatcatgac cgtcaatatc
    33721 gcccttgccg tcgccattgg cgctcgtctg ttaccgcgcg tcaaccgaat ggagtggact
    33781 attttagcct ctgccggaac catcggcgcg ctgtttggta cacctgttgc ggcggcgttg
    33841 atattttcgc aaaccttaaa tggcagtagt gaagttccgc tatgggatcg tctttttgcg
    33901 ccgttaatgg cggcagcagc tggtgcactt actaccggat tatttttcca tccccatttt
    33961 tcactgccca ttgcgcatta cggacagatg gaaatgaccg atattctcag cggtgcaatt
    34021 gtcgcggcga ttgccatcgc agcagggatg gtcgccgtat ggtgcttacc acggttgcac
    34081 gcgatgatga atcaaatgaa aaatccggtg ctcgtgctgg gtattggcgg atttattctc
    34141 ggaattctgg gggttattgg tggaccagtt tcgctgttta aagggctgga tgagatgcag
    34201 cagatggtgg caaatcaggc tttcagcacc agcgattact ttttgctggc ggtaattaaa
    34261 cttgccgccc tggtcgttgc tgccgccagt ggctttcgcg gtgggcgaat cttcccggca
    34321 gtgtttgtcg gcgtggcatt agggttgatg ctgcatgagc acgttcccgc cgttccagcg
    34381 gcaataaccg tttcctgcgc tattctcggc atcgtgctgg tggtaacacg cgatggctgg
    34441 ttaagtcttt ttatggcggc agtcgttgta cccaatacca cattgctacc gctgctctgt
    34501 atcgtcatgc ttccggcatg gctgttatta gcaggtaagc cgatgatgat ggtcaatcgt
    34561 ccgaagcaac agccacccca cgataatgtt tagcaaaaaa tgctctcctt tatgatttaa
    34621 gagttatggc gatgatacgg ggctttacct ccccgtaata ttgcgttaac aggccgctga
    34681 cggcttcgat cgtgaaggag aataacaatg ttcaggtcac tgtttctggc ggccgccctg
    34741 atggcattta ccccgcttgc agcaaatgca ggtgaaatca ccctactgcc atcgatcaaa
    34801 ttacaaattg gcgatcgcga tcattacggt aattactggg acggtggtca ctggcgagat
    34861 cgtgattact ggcatcgcaa ttatgaatgg cgcaaaaacc gctggtggcg tcatgataat
    34921 ggctatcatc gtggctggga taagcgtaaa gcatatgagc gtggctatcg tgaaggctgg
    34981 cgcgatcgtg acgatcatcg cggaaaaggc cgcggacatg ggcaccgcca ttaaacgcgt
    35041 cctccaatgg agcacaatgc ctgatgcgac gctagcgcgt cttatcaggc ctacaaaccg
    35101 gctctttaac gcgtagggcg gataaggcgt ttacgccgca tccgccaata gtgccagatg
    35161 cgacgctcat tcaactacaa tcccagcgcc gtccccacca acaaccagat attcagcgcc
    35221 acgaccagca ctacaatcac ccagcctgtc tgttttacgc gtttgctgtt caccagatcg
    35281 cccatcaact tgctgtcact ggtgaaaatc agcagtggaa ccagcgccag ggcgatacca
    35341 aaacttaaca gcacctgact cataaccaga atccgtgtcg gatctaatcc catcagaatg
    35401 acaataaatg acggcaacat ggtgactgta cgacgcaccc acagcgggat atggaagcga
    35461 atgaatccct gcatcaccac ctgccccgcc agtgtcccca ccaccgttga ggacagtccg
    35521 gcagcaacca gacttaaccc aaagaccgtt gccgcagcat ggcttaacag cggttgcagc
    35581 gtcagataag cctcatcaag atcggcaaca ccagtatgac cagaaaagtg gaacgccgcc
    35641 gcagctgtag ccatcatcac cagattgaca aaaccggcaa tcgtcatggc gatagccaca
    35701 tcccatttgg tggcggaata acgttgttga cgcgaaccgc catgtaaatg ctgagtgagc
    35761 gaagagtgca aataaatcac atgcggcata atcgtcgccc ctaacacgcc tgctgccagg
    35821 aagaccgcct ccgaagtagg taaactcggg atcaccattc ctttacccag ctgcgccagg
    35881 ttaggctggg agaaaatcaa ctcgacaatg taagccgcgg caacaaacaa cagtaacccg
    35941 ccaatcactt tctccagcgg tttttgcccg cgacgttgca gcattaaaat caggaaagtc
    36001 gcgatccccg tcaacaccgc gccctgcaac aacgaaacac caagaatgag tttaaaaccg
    36061 atcgccgcac caataaattc cgccaggtcg gttgccatcg caataatttc tgcctgaacc
    36121 caatagaacc acactacggg acgcggatag tgatcacgaa tctgctccgc cagattttta
    36181 ccggtggcaa tccctagttt ggcagagagg atctgaatca gcatcgccat caggttggcc
    36241 caaacgacaa cccacagtag ctgatagccg aagctagcac ccgcctgaat attggtcgca
    36301 aagttaccgg gatcgatata accaatcgcc gcaatgaacg caggtcccat taatgcgagc
    36361 ctcatcttgc gcgccgcccg tccgctgcta ctctcaacgc gatagttcgt catcttgtgc
    36421 ctctaaaaca tagcctttgc tatgtttcat gctatgctaa acgagaatga ttatcaaatt
    36481 catttaaatg gattgtggtg atttctctga tagaccaaga ttatgattac gaaaagattg
    36541 atgacttgaa tgttatggtt atgtttaatg ttagcacatt tacataactt tcagcttcca
    36601 tacacaacat agcagaaatg tatgacagat cactattttt gaagcctgtc acaggacgtc
    36661 attatagtgt gtgtcagatc tcgttttcct taaccatgtt acatagaatg tgcacggaaa
    36721 tttaacctgc ctcatatttg gagcaaatat ggaccgcgtc cttcattttg tactggcact
    36781 tgccgttgtt gtgattctcg cactgctggt aagcagcgac cgcaaaaaaa ttcgtatccg
    36841 ttatgttatt caactgcttg ttatcgaagt gttactggcg tggttcttcc tgaactccga
    36901 cgttggttta ggcttcgtga aaggcttctc cgaaatgttc gaaaaactgc tcggatttgc
    36961 caacgaaggg actaacttcg tctttggtag catgaatgat caaggcctgg cattcttctt
    37021 cctgaaagtg ctgtgcccaa tcgtctttat ctctgcactg atcggtattc tccagcacat
    37081 tcgcgtgttg ccggtgatta tccgcgcaat tggtttcctg ctctccaaag tcaacggtat
    37141 gggcaaactg gaatccttta acgccgtcag ctccctgatt ctgggtcagt cagaaaactt
    37201 tattgcctat aaagatatcc tcggcaaaat ctcccgtaat cgtatgtaca ccatggctgc
    37261 cacggcaatg tccaccgtgt cgatgtccat cgttggtgca tacatgacca tgctggaacc
    37321 gaaatacgtc gttgctgcgc tggtactgaa catgttcagc acctttatcg tgctgtcgct
    37381 gatcaatcct taccgtgttg atgccagtga agaaaacatc cagatgtcca acctgcacga
    37441 aggtcagagc ttcttcgaaa tgctgggtga atacattctg gcaggtttca aagttgccat
    37501 tatcgttgcc gcgatgctga tcggctttat cgccctgatc gccgcgctga acgcactgtt
    37561 tgccaccgtt actggctggt ttggctacag catctccttc cagggcatcc tgggctacat
    37621 cttctatccg attgcatggg tgatgggtgt tccttccagt gaagcactgc aagtgggcag
    37681 tatcatggcg accaaactgg tttccaacga gttcgttgcg atgatggatc tgcagaaaat
    37741 tgcttccacg ctctctccgc gtgctgaagg catcatctct gtgttcctag tttccttcgc
    37801 taacttctct tcaatcggga ttatcgcagg tgcagttaaa ggcctgaatg aagagcaagg
    37861 taacgtggtt tctcgcttcg gtctgaaact ggtttacggc tctaccctgg tgagtgtgct
    37921 gtctgcgtca atcgcagcac tggtgctgta aaaccataca taaaaaagcc ggggatcatt
    37981 ccccggcttt tttacgcctg ttaatcagct aatggctgcg ggcgaccaat caaataccct
    38041 tgcagatatt gcaccccgag cttatgcaat agcgcctgct gctgttgcgt ctcgacaaac
    38101 tccgcgacca cactcaatga cttcgctttc gccagatcgg taattgatct cacaatcatc
    38161 gcatccagcg tattcgtgac aatatctttc acaaagacgc catcaatttt gatgatatca
    38221 gcctgcaaac gctttaaccg ttcgtagttg gcatatccgg tgccaaagtc atcaatcgca
    38281 atccggaaac caaacttatg cagctgctcg atgttgtaca tgctgctttc tgcgttagaa
    38341 aacgcctgct cctcggtgat ctcaagaatg accgcctgcg gggagatgtg ataacgctta
    38401 aacagacgaa taatccgccc ggcaatattc ttttgcagca gcgtgagcgg cattaaattg
    38461 actgaaaagc gcggtccttt tttgtcgcaa gggtgtgttg ccagccactt caacaaggat
    38521 tccagcactt gcaaatcaaa acgcgcgcta aggttaaact gagcaataag gggcagaaac
    38581 ttatccgggg tcataatgcc gccgtcatat ttcagtcgcg cgaggatctc atcataacct
    38641 tcaccctctt tgttgcgaat tggctgggcg tagagcagca aatcaccctg atctaacgcc
    38701 gtgcgaatgg tattcagcaa tagcacctgt ttagtggtct gcccggaaac catctcctct
    38761 ctgctatcca gcgccagcac atgatgatgt gcgcaggatt gctccgccag ccagcttaac
    38821 tgccccaaca agggttgcag ggtttcctga tttccatcaa aacgccccca ggcagcacca
    38881 tagcccatat ccagcccggt attgttccag tgaatttgcc gactattcag gatgttaacc
    38941 atatgctgga gtcgcccttc cgtttccggc ccgctcagca ctaacagcag ttcactaccc
    39001 ggcaattgat acaacttttc gttttcctgc atcaacggca gcagcgtacg gcaaagccgt
    39061 aatgacgact cataaactca agattatcaa tgcgcaggca gcaaaaactc ttgcccgcct
    39121 cttgctccgg cgcttgttcc aacgcacgaa agttgggtag gagcgttaac ggatctgtta
    39181 gcgcctgcaa atgccagcgg cgattaagcc attcactgcg atgataaatg cgcaccatat
    39241 agagcaggca aacgctaaag gaaatcagca ccgcgagaat aaatgccagc gaatattcgg
    39301 tttccacccc ttgcaaaaag ttctggttgt aattcagaag gcaaagcgtt gaaacagccc
    39361 aggtgagatt taaaaacgga tagcgaagat taccgacccc gagggtaaag atgatgaaga
    39421 aaacgggtac caggtaaccg gcaataaagt cgttttcata aggtgtgcac aacagaagta
    39481 gcagcacgct taacgctgcc agccagctta aggtaaatgc gagtgagata gtagaaaagc
    39541 atgttgtaaa tcagcactgc ggtgaaaagg cttagtaaat caacgaccgt gaaaatggca
    39601 tccgcatcgc cgaaaaaggt ggatatcttt agcgggaaat caaagaagct ccccacaaga
    39661 tacatgctgc atttgatgcc gatcggcgtc accaaaccaa gccagaacag acgttgccag
    39721 acatatcggc tggtcaatcc ataacgccaa cgcgtcccca gctgccagcg taatattgcg
    39781 caggcacaca acacgacaaa cgtctggctg ccgagcatga ccgcagtctg taaaaaagag
    39841 agatgaaagt tccacagatt ggtgcaaaac atcccgcata aaacgggaac gacaccgcgc
    39901 cagccaaaga taaacaagat tgacagcatt acgcacagtg gcatccacgc cagaaagata
    39961 taactggaat ggataatggc aagtggcgaa ataaaacggg atagctgaat aagtaccacg
    40021 gtgagcgtaa acgctagtgt gaatatcttg atatttttta tcaggttatg ctccacaaac
    40081 atgagtacac tactctcact gatcggggtt ttcaggcaca caaataataa gtttattcgc
    40141 atcgcgaagc aaacaggggc cagcctgttg ccgatttttc atccaatgat gctttcacca
    40201 ggcggcgcta acccggacgt agactgtatt acaaaagcgg caaaaagcag agacaaaaaa
    40261 cccccgcttt gcagcgaggg ttggaaattt ggtggagcta agcgggatcg aaccgctgac
    40321 ctcttgcatg ccatgcaagc gctctcccag ctgagctata gccccacgat gcgtttacgt
    40381 accaaatttg ctgggtgcaa aatttggtgg agctaagcgg gatcgaaccg ctgacctctt
    40441 gcatgccatg caagcgctct cccagctgag ctatagcccc gtcacgtaaa gcttgtcgag
    40501 ttgacgggcg gcatcatatg aattccgccc gaatgtgtca acggcaaatt gcaacgtgta
    40561 gtttcaatcg ctgaaaaatc aggcaaatga acaattttgg aaaaccgctc gcattcagta
    40621 gttattcatg tcacggtttc ctgtaaagtg gtgttataaa atgaactact aatagaccca
    40681 catacattca gggaattgtt atgttcaagg agaggatgac accagatgaa cttgccagac
    40741 tgaccggcta tagccgccag accattaata aatgggtacg caaggaaggc tggacaacgt
    40801 caccaaaacc tggcgtccag ggtggcaaag cccgactggt tcacgtcaat gaacaggttc
    40861 gtgaatatat tcgcaatgct gaacgtccag aaggtcaggg agaagcgcct gccctttctg
    40921 gtgatgcgcc gcttgaagta ttgctggtga cactggcgaa agagatgacg ccagttgaac
    40981 aaaaacagtt tacatccttg cttctgcggg aagggattat cggattgtta caacgcttag
    41041 ggattcgcga tagcaaataa tatgaaaaga ttacgcaata aaatgaccac cgaagaactg
    41101 gctgaatgcc tcggtgtggc taaacaaact gttaaccgtt ggaccagaga aaaaggctgg
    41161 aaaacggaaa aatttcccgg cgtgaaaggc ggtcgtgcca gacttattct ggtcgatacg
    41221 caagtttgcg agtttattca gaacacgcca gccttccata acacaccaat gttgatggaa
    41281 gctgaagaga gaattgctga atacgctcct ggagcacgcg ccccggcgta tcgacaaatc
    41341 atcaacgcga tagataatat gacagacatt gagcaggaaa aagtcgcgca atttttgtca
    41401 cgtgaaggaa ttcgcaattt cctcgcccgt ctcgatattg acgaatcagc ataaataaaa
    41461 aacggcagga tattatctcc tgccgtttat ctttttacac gctaattact gctgattttc
    41521 gcgttcagca ataaaatcca gcgctttgtt gatacgctcg atactgcggg tcttaccaat
    41581 tgcgtgaacg gtaacatcca gtgctggaga ctgccccgca ccggttacgg cgacacgcag
    41641 cggcatacca actttaccca tacccacttc cagctcatcc gccgtcgcct gaatagcgtg
    41701 atgaacgttt tcagcggtcc agtcagtaat cgcggccagt ttgtcacgaa ccacttccag
    41761 cggctgacgc gctaccggac gcagatgttt tttcgccgca tcggcatcga actcagcaaa
    41821 atcttcgtag aaataacggc agctctgtgc catctctttc agcgtcttgc agcgttcgcc
    41881 cagcagtttc accagatcag ccagctgcgg gccgttacgg gtatcgatat tttcctgctc
    41941 aatgtgccac tgtaagtgag tagcaacata ctccggcggc agcgcgttaa tgtagtgatg
    42001 gttcagccac agcagcttgt cggtgttgaa cgcactggca gatttgctga cggcattcaa
    42061 agtgaagtat ttgatcatct cttcacgagt gaagatttcc tgatcgccgt gggaccagcc
    42121 cagacgcacc agatagttca gcagtgcttc tggcaaataa ccgtcatcac gatactgcat
    42181 tacgctgact gccccgtgac gtttggacag ttttttaccg tcatcgccat tgatcataga
    42241 aacgtgcgcg taaaccggca ccggcgcttt cagggcctta agaatgttga tctggcgcgg
    42301 cgtgttgttg atatggtctt cgccacggat aacgtgggtg atttccatat cccagtcatc
    42361 gacaaccaca cagaagttat aggttgggga accatcggta cggcggataa taagatcgtc
    42421 cagttcctgg ttgctgaact cgatcggacc acggatctga tcgtcaaaaa caacagaacc
    42481 ttcctgcggg ttagcaaaac gtacaacaca cggttcatca tcagcatgat gctcatggct
    42541 gtggcggcag cgaccgtcat aacgcggctt ctcacctttc gccatttgct cttcgcgcag
    42601 cgcttccagg cgctctttag agcaatagca tttataagca gtgccctctt ccagcatctg
    42661 atcgatcacc gcgttgtagc gatcaaaacg tttggtctgg tagtacggac cttcatccca
    42721 ctccaggctc agccagttca tgccatccat aatggcttcg atagcttccg gcgtggaacg
    42781 ctcaagatcg gtgtcttcaa tacgcagcac gaactcaccg ccgtggttac gtgcaaaaag
    42841 ccaggagtaa agagcagtac gcgcgccgcc aacgtgcaga tagcctgttg ggcttggcgc
    42901 gaagcgagtt ttgattttca tgaaatggcc ttacgtttag aaagatgccg acaaccggca
    42961 aatcctggaa aaattgagtg ggcgatattc tatcactccc gcctgattcc tcaatgtagt
    43021 tcgggtttct accttacgca ttattgtttt cgttgacaaa ttgcgcaaca cggtctgttt
    43081 tgcgatcatc ttgcttaaat ttacgacgaa cgaacaattt ctttagaaaa tgcgttgact
    43141 cattttgaac tctccctata atgcgactcc acacagcggg ggtgattagc tcagctggga
    43201 gagcacctcc cttacaagga gggggtcggc ggttcgatac cgtcatcacc caccaactac
    43261 tttatgtagt ctccgccgtg tagcaagaaa ttgagaagtg ggtgattagc tcagctggga
    43321 gagcacctcc cttacaagga gggggtcggc ggttcgatac cgtcatcacc caccacttcg
    43381 ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc
    43441 ctgcacgacc caccaatgta aaaaagcgcc ctaaaggcgc ttttttgcta tctgcgatac
    43501 tcaaagattc gaacctgcag caggtttgag ttgagcgcag cgaaacaacg gagccgctcg
    43561 cggcgacggc ccgaagggcg agcgaagcga gtcatcctgc acgacccacc aatgcaaaaa
    43621 agcgccctaa aggcgctttt ttgctatctg cgatttgtaa gattgatttt tcttctcaag
    43681 ttccttgttc ttctaccgat attacctcgt acagtgacga ggactgaaca atggccacta
    43741 tctcttcaac ttcaattcct tccatacaaa cacagagtag taacagggca tcacaaggta
    43801 gcgatgttgc ttcacaaatc gcgcggataa gtcagcaaat catcaaactc actcaacaaa
    43861 ttaaagagat tgtagacact agcggcagtg ctgaagacaa gcaaaaacag gccgaattga
    43921 ttcaacagca aatcacccta ctggaaacac aactggcgca gttgcagaaa cagcaggcag
    43981 aaaaggcgca ggagaaagag cagcgtttgt ctctgaatgt atcccttcta aatcctgttg
    44041 aaaaaacgac tcatatcgat atctatatct gatacaacgg atccccttcc cgaccagcca
    44101 ttagcgcatt gccctggtct cgtaccacgt cccataacgc ttcagcggcg gtggaaagtg
    44161 aacggttctt cctgcgcacc agcatcaact ggcgttcaac caccggcgta atgcgcttaa
    44221 ccaccagtgg gctaccttca ggcaatggta gcgccagtgc aggaagaata ctgatgccga
    44281 tgcctgcggc taccatcggg aacagcgtcg ccggatgtcc aatctcctgc acaatattcg
    44341 cctgaatacc attgcgcgcc agcgctgcgt caatcagcgg tcggctgcct gacgcgtaat
    44401 cctgcaacac caatttcgct ccctgtaatg cctgccaggg tacataatct tccacggcca
    44461 aagcgctatc gcgatggcaa agcagaaaga aaggctcgga aagaatcgct tcacattgca
    44521 gatcgcccac tgggcctgga tcgatgacga tgccaaagtc gacatctccc tggcgaatac
    44581 tttccatcac ccactgctgc ggacgatcat gcaggacaaa ctgaatatct ggatagcggc
    44641 gatggctttc agcaatgcat tgcggaataa gatgcgcgga aatggtctgg ctggcagcga
    44701 cccgaacttt tccgctcagt tgttgcccca tacgcccggt atcgcgcaac gtgctgttaa
    44761 gttcatccag cagtcgctca agacgcaaag ccaactgctg ccctgcatct gtaagcacca
    44821 cttcacgcgt ggttctgtcc agcaggcgaa caccagtatg attttccagc tccttcacac
    44881 tgtgacttac tgccgactgg ctcaagccga tacgctctcc tgcacgacta aaacttttct
    44941 cctgcgctac tgtgacgaaa acctttaatt gttttaaaga ataattcatc tgttttcttc
    45001 atgaatggat gcaataaatc aattttattt ctcaaactgg aagaagcaca atagaaccat
    45061 cgatcatctg gagtctttat gaaacttttt cgtatcctcg atcctttcac cttaaccctg
    45121 atcacggtgg tgttgctggc ctctttcttt ccggccagag gcgatttcgt ccccttcttt
    45181 gaaaatctga ccaccgcagc tattgccctg ctgttcttta tgcacggcgc gaagttgtcg
    45241 cgtgaggcga ttattgctgg cggtggtcac tggcgactgc atttgtgggt aatgtgcagc
    45301 accttcgtgc tgtttccgat tctgggtgta ctgtttgcct ggtggaaacc ggtaaatgtc
    45361 gacccgatgc tctactccgg ttttctctac ttgtgcattc tcccggctac cgtgcagtct
    45421 gcaatcgcct tcacgtcaat ggcgggcggt aacgtcgcgg cggcggtttg ttctgcgtca
    45481 gcatccagcc tgctggggat tttcctttca ccattgctgg ttggtctggt gatgaatgtt
    45541 cacggtgcaa ggggcagcct tgagcaggtc ggtaaaatta tgctgcaact gctgctgccg
    45601 tttgtgttgg ggcatctttc ccggccgtgg attggtgact gggtgtcgcg caataaaaaa
    45661 tggattgcga aaactgacca gacgtccatt ctgttggtgg tttatacagc gttcagcgaa
    45721 gccgtcgtta atggtatctg gcataaagtt ggctggggat cattgctgtt tatcgtggtg
    45781 gtcagctgcg ttcttctggc tatcgtgatt gtagttaacg tctttatggc acgccgactg
    45841 agcttcaata aggcagatga aattactatc gtcttttgtg gttcgaaaaa gagtctggca
    45901 aatggcatcc cgatggcaaa cattctgttc cccacatcgg tgatcggtat gatggtgctg
    45961 cccctgatga ttttccatca gatccaattg atggtctgtg cggtgctggc gcgtcgatac
    46021 aaacgccaga ccgaacagtt acaggcgcag caggaaagca gcgccgataa agcttaaagc
    46081 ggacgcttca ggggctggac cagttgcgtc agcccctcgg ttttaatcag cagcgtgatt
    46141 tgcattagct caccgagctt ccctgccggg aactcatctt tgcgggcaaa ccacagcaga
    46201 tactcctccg gcaggtcaat taagcgacgc cctttgtatt tgccaaacgg cattatcgta
    46261 ttggctattt caatcagctg ctctttttcc atctcagcta cccagcaaac gcagcatttc
    46321 cgcttcgtcg atgacttcaa tgcccagttc ctgcgccttc gccagtttag atcctgcagc
    46381 ttcacccgct atcaccagat cggttttctt cgacacgctg cccgcgactt tcgcgcccag
    46441 ttcgaccagt cgagctttag cgtcatcacg cgacatctgg cttaagctgc ccgtaagcac
    46501 cacggtttta ccaacaaacg ggctgtcaat ctcttccgcg ttgataacga tcggcgcagg
    46561 ccagtgaaca ccttccgcca acagctcgct gatgacattg cggttgcttt cttcggcaaa
    46621 gaagttgtga acgtgggatg caacgacaat gccaacatca ggcacctttt gcagctcttc
    46681 aatcgaagcg gcttccagcg cttccagcgt gccgaaatat gccgccagac ctgctgcggt
    46741 ggcctcgccg acttcacgga tgccaagtgc atagaggaag cgagcaaagg tggtttcttt
    46801 cgctttttcc agcgcgttaa ccacgttttg tgccaatttt ggccccatac gctccagtcc
    46861 ggtcagtttg cctgcggtga gtttgaacag atctgccgga gtatggacat attctttttc
    46921 aaccagctga tcgatgattt tgtcgcccat tccgtcaaca tccatcgcac ggcgggaaac
    46981 aaagtgtttc agcgactctt tacgctgcgc accgcaaatc aggccaccgg tacagcgggc
    47041 aaccgcttca ccttccacac gctcaacgtc agaaccacat accggacaat gcgtcgggaa
    47101 tacaacctca cgggtatctt ccgggcgttc agaaagcacg acgttaacca cctgcgggat
    47161 cacgtcgcca gcgcggcgaa tcaccacttt atcgccaatg cgtaaaccaa gacgttcgat
    47221 ttcatccgca ttgtgtaagg ttgcgttact caccagcacg ccagcaacat ggacaggttc
    47281 cagacgcgca acaggcgtaa tcgcgccagt acgcccaacc tgaaactcga cgtcacgcac
    47341 aaaggtcatc tgctcctgcg ccgggaattt aaacgcaacc gcccagcgcg gggcacgcgc
    47401 gacaaagcca agctgctcct gctgttccag tgagttgacc ttaatcacca cgccgtcgat
    47461 atcaaagccc agcgtcgggc ggtcttcttc cactttgtga tagaacgcca gcacttcttc
    47521 cgccgattca caaagcgtta cccgatcgct gaccggcacc ccccactttt taaattgcag
    47581 taaacggcca agatgagtat ccggcagctc gccaccttcc agaacaccaa cgccatagca
    47641 gaaaaaagtg agcggtcgct tcgctgtaat acgcggatca agctgacgca gtgaaccagc
    47701 tgccgcatta cgtgggttag caaaaacttt cccgcccgtg cgtcgcgcat cttcgttaat
    47761 cttttcgaac cccgcctgcg gcaggaacac ttcaccacgc acttccagac gcgccgggat
    47821 attctctccg tgcagcttca gcggaatggc gcgaatagta cgcacattag acgtgatatc
    47881 ttccccggtg gtgccatcgc cacgggtcgc ggcactgact aaaacgccat tttcatacag
    47941 aatactgacg gcaaggccat ccagcttcag ctcacagcac caggtgactt tctcgttgtt
    48001 tttcagacgg tcctgcacac gtttgttgaa agcaagaaag ctttcttcat caaaaacgtt
    48061 atccagtgac agcattggta cttcatggcg tatctggctg aaagctgcca gcggcgcagc
    48121 acctacacgt tgggtaggcg aatcaggcgt aatcagttct ggatgtttgg tttccagctc
    48181 gcgcagttcg cgcatcagcc tgtcgtattc agcgtcggga atttccggcg catccatcac
    48241 atgataaaga tattcatgat ggcaaagcgt cgttcgcagt tctgtcagtt gttgttcgat
    48301 tgattccata tcgcaccatc aatgctaaaa acccccgaca agcgggggtt cgaagaggag
    48361 ttaatttgcc ttaagtgtat caggcgttgg cgtctttgac ttcgcggatg atgtcctggt
    48421 actcgcgcaa tttctgcgga gtcatcatac ggcgctgatc gtcaagcacg acaccgccca
    48481 cttcatcggc aatatgctgc gcagattgca gcatcagctt gaagttctgc agctcgtcac
    48541 cgtaagacgg tacctgcata aagatagtga cacccggagt agtgaaatcc ttcatttcag
    48601 gatcaaaggt tcccggtttc accatattcg ccaggctgaa taacgccggg ccgctgccat
    48661 ccgggctaag atgacgatgg taaatattca tatcgccaaa aatgaagccc gcttgttgaa
    48721 tgctgttaag aagcagttca ccgtttagct cgctaccgtg atgcgccgcg acgttcatga
    48781 taatcaccgc ttctttgcgc ttcggtttat ccataactgg agccggttcc gctacaggct
    48841 caggctgtgg tgccgctacg ggttctgcag gctggaaagc ctgttgtgcc ggttgcggtg
    48901 ctgaatgcac aggctgcggc gctggcgcga cctgtggcga aactggctgc tgcaacggct
    48961 gttcaggctg cggctgatag gcaggctgct gcaccggctg cgctggacgc ggagcatgtt
    49021 gcggcggtac ctgcgcttca ggcggctgct ggaccggttg acgcggctgc gcagacgcat
    49081 aaggcggtag gtactggtgt tgcggcgacg gacgagcagc ctcatgctcc tgagcgttag
    49141 ccggggcatg attcacgcgg tgaacacgaa cctcaccaac gccctcatca tcttcgacat
    49201 cctcgtcata agaatcgtcg tcacgttttg acttcattcg ttttaatggc cgatcgcgga
    49261 acatagaaga tcgttcttta cggctggtcc agaaaccatg taccagtaaa gcgattatgg
    49321 cgatcgcgcc aacaatgatt aatatcagac gcaaatcctg catcattata ttctctgttg
    49381 ttctaacacc ttgccaccac ggcaaacatt tactcactaa gagtatttgc cgattacctc
    49441 aagtgcaagt gcactattaa ctttcacagc acaaagatag atgaaatcgt gctttttgct
    49501 gttttttcga acatatccta actgtccatt gcgcaattac ccggtaaaat acgcagaatt
    49561 ttcctgggat tggtcaaaag gagctcatcc tgattatggt ttcatcattc acatctgccc
    49621 cacgcagcgg tttttactat tttgcgcaag gctggaagct cgtctcgcaa cctgggattc
    49681 ggcgtttcgt tattttaccg ctgctggtca atattttgtt gatggggggc gcattctggt
    49741 ggctctttac acagctcgat gtctggatcc cgactttcat gagttacgtt ccggactggc
    49801 tacaatggct gagttatctg ttgtggcctc tggctgtcat ctctgtgctg ttagtgtttg
    49861 gctatttctt ctccacgatt gctaactgga ttgccgctcc gtttaacggt ttattggctg
    49921 aacaactgga agcacgattg actggcgcta caccgccaga taccgggatt ttcggtatca
    49981 tgaaagatgt gccgcgaatc atgaaacgcg aatggcaaaa atttgtctgg tatctgccgc
    50041 gcgcaattgt attactaatt ctttacttca tccctggtat tgggcaaacc gtcgcgccgg
    50101 tactgtggtt cctgtttagc gcctggatgt tagccatcca gtattgcgat taccccttcg
    50161 ataaccacaa agtgccgttt aaagagatgc gcaccgccct gcgcacacgc aaaatcacca
    50221 atatgcagtt tggtgcttta accagcctgt ttacgatgat cccgctgctt aatctgttca
    50281 tcatgcccgt tgccgtttgt ggcgcgacgg cgatgtgggt cgattgctat cgcgataaac
    50341 acgcgatgtg gcggtaacaa tctaccggtt attttgtaaa ccgtttgtgt gaaacagggg
    50401 tggcttatgc cgccccttat tccatcttgc atgtcattat ttcccttctg tatatagata
    50461 tgctaaatcc ttacttccgc atattctctg agcgggtatg ctacctgttg tatcccaatt
    50521 tcatacagtt aaggacaggc catgagtaag atttttgaag ataactcgct gactatcggt
    50581 cacacgccgc tggttcgcct gaatcgcatc ggtaacggac gcattctggc gaaggtggaa
    50641 tctcgtaacc ccagcttcag cgttaagtgc cgtatcggtg ccaacatgat ttgggatgcc
    50701 gaaaagcgcg gcgtgctgaa accaggcgtt gaactggttg aaccgaccag cggtaatacc
    50761 gggattgcac tggcctatgt agctgccgct cgcggttaca aactcaccct gaccatgcca
    50821 gaaaccatga gtattgaacg ccgcaagctg ctgaaagcgt taggtgcaaa cctggtgctg
    50881 acggaaggtg ctaaaggcat gaaaggcgca atccaaaaag cagaagaaat tgtcgccagc
    50941 aatccagaga aatacctgct gctgcaacaa ttcagcaatc cggcaaaccc tgaaattcac
    51001 gaaaaaacca ccggtccgga aatctgggaa gataccgacg gtcaggttga tgtatttatt
    51061 gctggcgttg ggactggcgg tacgctgact ggcgtcagcc gctacattaa aggcaccaaa
    51121 ggcaagaccg atcttatctc tgtcgccgtt gagccaaccg attctccagt tatcgcccag
    51181 gtgctggcag gtgaagagat taaacctggc ccgcataaaa ttcagggtat tggcgctggt
    51241 tttatcccgg ctaacctcga tctcaagctg gtcgataaag tcattggcat caccaatgaa
    51301 gaagccattt ctaccgcccg tcgtctaatg gaagaagaag gtattcttgc aggtatctct
    51361 tctggagcag ctgttgccgc agcgttgaaa ctacaagaag atgaaagctt taccaacaag
    51421 aatattgtgg ttattctacc atcatcgggt gagcgttatt taagcaccgc attgtttgcc
    51481 gatctcttca ctgagaaaga attgcaacag taatgccagc ttgttaaaaa tgcgtaaaaa
    51541 agcacctttt taggtgcttt tttgtggcct gcttcaaact ttcgcccctc ctggcattga
    51601 ttcagcctgt cggaactggt atttaaccag actaattatt ttgatgcgcg aaattaatcg
    51661 ttacaggaaa agccaaagct gaatcgattt tatgatttgg ttcaattctt cctttagcgg
    51721 cataatgctt aatgacgtac gaaacgtcag cggtcaacac ccgccagcaa tggactgtat
    51781 tgcgctcttc gtgcgtcgcg tctgttaaaa actggcgcta acaatacagg ctaaagtcga
    51841 accgccaggc tagactttag ttccacaaca ctaaacctat aagttgggga aatacaatgt
    51901 tccagcaaga agttaccatt accgctccga acggtctgca cacccgccct gctgcccagt
    51961 ttgtaaaaga agctaagggc ttcacttctg aaattactgt gacttccaac ggcaaaagcg
    52021 ccagcgcgaa aagcctgttt aaactgcaga ctctgggcct gactcaaggt accgttgtga
    52081 ctatctccgc agaaggcgaa gacgagcaga aagcggttga acatctggtt aaactgatgg
    52141 cggaactcga gtaatttccc gggttctttt aaaaatcagt cacaagtaag gtagggttat
    52201 gatttcaggc attttagcat ccccgggtat cgctttcggt aaagctctgc ttctgaaaga
    52261 agacgaaatt gtcattgacc ggaaaaaaat ttctgccgac caggttgatc aggaagttga
    52321 acgttttctg agcggtcgtg ccaaggcatc agcccagctg gaaacgatca aaacgaaagc
    52381 tggtgaaacg ttcggtgaag aaaaagaagc catctttgaa gggcatatta tgctgctcga
    52441 agatgaggag ctggagcagg aaatcatagc cctgattaaa gataaacaca tgacagctga
    52501 cgcagctgct catgaagtta tcgaaggtca ggcttctgcc ctggaagagc tggatgatga
    52561 atacctgaaa gaacgtgcgg ctgacgtacg tgatatcggt aagcgcctgc tgcgcaacat
    52621 cctgggcctg aagattatcg acctgagcgc cattcaggat gaagtcattc tggttgccgc
    52681 tgacctgacg ccgtccgaaa ccgcacagct gaacctgaag aaggtgctgg gtttcatcac
    52741 cgacgcgggt ggccgtactt cccacacctc tatcatggcg cgttctctgg aactgcctgc
    52801 tatcgtgggt accggtagcg tcacctctca ggtgaaaaat gacgactatc tgattctgga
    52861 tgccgtaaat aatcaggttt acgtcaatcc aaccaacgaa gttattgata aaatgcgcgc
    52921 tgttcaggag caagtggctt ctgaaaaagc agagcttgct aaactgaaag atctgccagc
    52981 tattacgctg gacggtcacc aggtagaagt atgcgctaac attggtacgg ttcgtgacgt
    53041 tgaaggtgca gagcgtaacg gcgctgaagg cgttggtctg tatcgtactg agttcctgtt
    53101 catggaccgc gacgcgctgc ccactgaaga agaacagttt gctgcttaca aagcagtggc
    53161 tgaagcgtgt ggctcgcagg cggttatcgt tcgtaccatg gacatcggcg gcgacaaaga
    53221 gctgccatac atgaacttcc cgaaagaaga gaacccgttc ctcggctggc gcgctatccg
    53281 tatcgcgatg gatcgtaaag agatcctgcg cgatcagctc cgcgctatcc tgcgtgcctc
    53341 ggctttcggt aaattgcgca ttatgttccc gatgatcatc tctgttgaag aagtgcgtgc
    53401 actgcgcaaa gagatcgaaa tctacaaaca ggaactgcgc gacgaaggta aagcgtttga
    53461 cgagtcaatt gaaatcggcg taatggtgga aacaccggct gccgcaacaa ttgcacgtca
    53521 tttagccaaa gaagttgatt tctttagtat cggcaccaat gatttaacgc agtacactct
    53581 ggcagttgac cgtggtaatg atatgatttc acacctttac cagccaatgt caccgtccgt
    53641 gctgaacttg atcaagcaag ttattgatgc ttctcatgct gaaggcaaat ggactggcat
    53701 gtgtggtgag cttgctggcg atgaacgtgc tacacttctg ttgctgggga tgggtctgga
    53761 cgaattctct atgagcgcca tttctatccc gcgcattaag aagattatcc gtaacacgaa
    53821 cttcgaagat gcgaaggtgt tagcagagca ggctcttgct caaccgacaa cggacgagtt
    53881 aatgacgctg gttaacaagt tcattgaaga aaaaacaatc tgctaatcca cgagatgcgg
    53941 cccaatttac tgcttaggag aagatcatgg gtttgttcga taaactgaaa tctctggttt
    54001 ccgacgacaa aaaggatacc ggaactattg agatcattgc tccactctct ggcgagatcg
    54061 tcaatatcga agacgtgccg gatgtcgttt ttgcggaaaa aatcgttggt gatggtattg
    54121 ctatcaaacc aacgggtaac aaaatggtcg cgcctgtaga cggtaccatt ggtaaaatct
    54181 ttgaaaccaa tcacgcgttc tctatcgaat ctgatagcgg cgttgaactg ttcgtccact
    54241 tcggtatcga caccgttgaa ctgaaaggcg aaggcttcaa gcgtattgct gaagaaggtc
    54301 agcgcgtgaa agttggcgat actgtcattg aatttgatct gccgctgctg gaagagaaag
    54361 ccaagtctac cctgactccg gttgttatct ccaacatgga cgaaatcaaa gaactgatca
    54421 aactgtccgg tagcgtaacc gtgggtgaaa ccccggttat ccgcatcaag aagtaattct
    54481 tgccgcagtg aaaaatggcg cccatcggcg ccatttttta tgcttccgcc agcggcggca
    54541 aaatcaattc atcgctctca tgctgctggg tgtagcgcat cacttccagt acgcgcaacc
    54601 ccgcacggtg caccgcatcg tttaacgcct tccctttcag caaaccactg ataagctgag
    54661 cacaaaacag gtcgccagtc cctttcaggt cggtttttac ccgtgaatgg gaaatgacat
    54721 tcacgctgtc ggcactgacc accaccacct gcatctcctg attttcttca ttaccggagg
    54781 cgctggtaat caccacccat tttaatgtgt ctgaaagcag actttttgcg gcagcaatgg
    54841 cactgtcgag atcgcggcaa tttttaccgg tcaggatttc cagctcaaag atattggggg
    54901 taattccctg cgccagcggc agtaaatatt gtcgatacgc ctcgggaagg tcaggtttga
    54961 cataaattcc gctatcaata tcgccaatca ccggatcgac catgatcaat aggtcaggat
    55021 ggtctttgcg tagcgcagtc agccactcgg caaggatttt gatttgcgat gccgttccca
    55081 tatagcccgt ggttacagca cgaagttggc gcagcgcatc acgctcctga agcgcacgca
    55141 aatagccgct aaaccattcg tccggaatcg caccaccgta gaaagtgtca taatgcggcg
    55201 tattgctcag caataccgtc ggcacggcaa agacattcaa gccgttctgt tttatagcag
    55261 gcaccgcaat gctgttgcct acgcttccgt acactacctg cgactgcacg gcgacgatat
    55321 ccgcctgcag cgccctactc ttatcgttaa acaacaacaa actactcatt taattttttc
    55381 tccttgccga tgatcctcat cgtattccaa ccgaaacttt acctgattct ggcagtcaaa
    55441 tcggctatta caaaacaaag ataaggtaat tcagtgaaga aaatcatttg tctggccatt
    55501 acgctgctaa tgacactccc cgcttacgcg aagttaactg ttcatgaaga agcccgcatc
    55561 aacgccatgc tggagggatt agcacagaaa aaggatttga tatttgtgcg caacggtgat
    55621 gaacatacct gcgatgaagc ggtttcccac ctgcgtctga agctcggcaa tacccgtaac
    55681 cgcattgaca ctgccgagca gtttattgat aaggtagctt cgtcgtcatc gattaccggg
    55741 aagccgtata tcgtgaagat ccccggtaag agcgatgaga acgcacagcc ttttttacat
    55801 gcgttaattg cgcagacgga taaaacggtg cctgcggaag gaaattaatc cgctttggta
    55861 aggcatttac aggaggtaac atgaaaaaat gctttattta tcacgatgaa aaatcgaata
    55921 aattttggtg gatagattac gaaggggata gtttagctgt caactatggc aaggtaggta
    55981 gtattggtaa attccataca aaagaattcg ataatgaaga acagtgtctg aaagaagcca
    56041 gtaaattgat tgccgcaaaa atgaaaaaag gatatcaaga aactccagac tttaacttca
    56101 tggatctcta ctattttgat gatgaagaga ttgggttaca tcttaaaacg tcacacccaa
    56161 acttccagtg ccattttact gatccacttt atatgtgttg ctgggatgaa gaatctcctt
    56221 ttggtagcga tgaaggtgct gatgctctaa acgaacttga aaatagcctc cgttctggac
    56281 tgtgctgatt tccctcaaat gttaattgaa actatgtggg gtatgaaata catcgctatg
    56341 gacagtattc ttgaagagga tgtgcgtgcg caattactag ccgatgaaat gagctctatc
    56401 cagagcaata tgattaccta cgcgactgca ttcggtcaga tcaaagtcat gggtaaaatc
    56461 tcccataaac ttaaaaagat gggactcaat gcactagcgc gtcatcagct taccgcaaaa
    56521 attcttcaat ggggtgacgg tcaggactca ccaatacttc aaaaaatgat tgatgacctt
    56581 actgcttttc cacacgaaaa ttaaaaactg catttgtcgg cagcaacaac tgccgacaaa
    56641 tcagattatt gacaaagtgc actttgttta tgccggatgc ggcgtaaacg cctgatccgg
    56701 cataaaaata ttgctaattc aatatattgc agaaacattg taggcctgat aagcggagcg
    56761 catcaggcag ttttgcgttt gtcatcagtc tccaatgcta ttaatcctta aatccctgcc
    56821 cccagactaa aatgctcttc cccaaacact ccggtggaga ggtagcgatc gccacgatcg
    56881 cagatgatcg ctaccaccac cgcaccaggg ttagcttttg ccacccgcag tgctccagca
    56941 accgcgccgc ctgagctgac gccacagaat attccttccc gcaccgccag ttcgcgcatg
    57001 gtgttttccg catcgcgctg atgaatatcc agcacctcat ccaccagcgc agcgttgaaa
    57061 atccccggca gatattccgc aggccagcgg cgaatgcccg gaatactgct gccttcttcc
    57121 ggttgcaggc caacaatggt caccggtttg gattgttcgc gcataaagca tgaaacgccg
    57181 gtgatagtgc cggtcgtccc catgctggag acaaaatgag tgatgcgccc gccggtttgc
    57241 tgccagattt ccggcccggt ggtggtgtaa tgcgcataag ggttatcggg attattgaac
    57301 tgatcgagca gctttccttc gccacgattc gccatctcca gcgccagatc gcgcgcacct
    57361 tccatgccct gctctttggt gacaagaatc agttccgcac cataagcacg catcgccgca
    57421 cggcgttcct ggctcatgtt gtcgggcatc agcaatttca tgcgatagcc tttcagcgcg
    57481 gcaatcattg ccagcgcaat gccggtgtta ccactggtgg cttcgattaa gacatcaccc
    57541 ggtttaattt ccccgcgctt ttccgcctcg acgatcatcg aaagtgccgc acgatctttc
    57601 accgaacctg ccgggttatt gccttccagt tttaaccaca cttcactgcc gttatccggc
    57661 cccattcgct gcaacttcac cagaggcgta ttgcctattg tttgttctaa tgtactcaca
    57721 atctctatcc atacgtggtg ttgcctgatg cgacgcttgc gcgtcttatc aggcctacag
    57781 gttacaaacc ttgccattaa aaagcccgga tcgcgggaag cgcctccggg cgtttaacat
    57841 tcactcaacc tatcaggcgc tttgtgcgag agcaagttcc tcatcgcggg tttcaatacg
    57901 ctcgtcgccg ttatacagcc gcgcatgttg cagaccaacg aataaacgct cgccatgctg
    57961 cggggcatcg tcgccatgca tcacgaccgt cagcggttcg ttgtaccacc ccagcggctg
    58021 caccactaat tgggtgtagt gacctttcgg gctggcttcc agtacctgta ccggcagcgg
    58081 cgaatcgaga ctggtacggc ggctgatatc cacttcccac gggcgcagga agagatccac
    58141 cggcccctga tacgcaggtg tgtagcccag cggccagcga tgcgcgccaa catggaactg
    58201 cccgccgcga atggttccct gcaggcggtt cacttcgccc ataaattcga gtacaaaacg
    58261 ggtcgctggt tcgcgccata cctgattcgg cgcatcagcc tgttcaatat tcccctgact
    58321 catcaccact acacgatcgg ctacttcggt cgcctcttcc tggtcgtggg tcacaaacac
    58381 gctggtgaat ttcagctctt catgcagttg acgcagccag cgacgcagct ctttacgcac
    58441 ctgcgcatcc agcgcgccga acggttcatc aagcagcaga atttgcggtt ctacagccag
    58501 cgcgcgcgcc agcgccacac gctgtttctg gccgccggaa agctgcgccg gataacgatc
    58561 cgccagatgc gcaagctgga ccatttccag caattttgtc actttcgctt tgatggctgc
    58621 ggcattcggg cgctcgcgac gcggcagcac cgtcaggcca aaagtgatat tgtcgaatac
    58681 cgtcatatgg cggaacagcg cgtaatgctg gaacacgaaa ccgactttac gatcgcgcgc
    58741 gtgcaggcga ctcacgtcgg tgccgtggaa gcgaatatgc ccgctggttt gatgctccag
    58801 cccggcgata atccgcagca gcgtggtttt cccggaaccg gacggcccca gcaacgcgac
    58861 catctgacca gaaggaatat ccagtgagat atcgttcagc acctgggtgc gaccaaaaga
    58921 cttcttgata ttggcaatct caatgctcat gatgctcctc ctgctgcgcg cgtttttcct
    58981 gattctccag gcgccactgc aacatactct ttaaaaacag ggtgataatc gccatcaacg
    59041 tcaacagcgc ggcggcggta aaggagccga cggtgttgta gtcctgctcc aacaattcaa
    59101 tctgtaacgg cagcgaaagt gtttcgccgc gaatcgagcc ggaaaccacc gacactgcac
    59161 caaactcgcc aatcgcgcgg gcgttggtca gcaccacgcc gtaaagcagc gcccagcgaa
    59221 tattcggcaa tgtgacgcga cggaacatct gccagccgga cgcgccaagc aaaatcgccg
    59281 cttcgtcttc ctggctgccc tggcttaaca tcaccggcac cagctcgcgc accacaaacg
    59341 gacacgtcac aaagatggtg accagcacca ttccgggcca ggagaacata atttgcagat
    59401 tatgttcatc cagccagccg ccgagcgggc cgttggagcc gtagaacagc agatacacca
    59461 gaccggcaac caccggcgat acagcaaacg gaatgtccag tagcgtcagc agtaactggc
    59521 gtcccgggaa gttaaagcgc gtcaccagcc aggccagcag aatgccgaac accaggttta
    59581 ccggtacggc aatcagcgcg atcatcaccg tcagccagat ggcgtgcagc atgtccggat
    59641 cggccagatt ctgtaaaacc ggcatcagcc ccttgctgaa tgcctgcacg aagatgtaaa
    59701 tcatcggcac cagcaggatg aacgccgaaa ccagcatccc gatgccaatc agaaaccatt
    59761 tgccccagtt aatcgggcgc gcgtcataac gcttcaattg ggtaacttcc gccattaatg
    59821 acctaccaca cgccgaccaa agcgactttg cagagtgtta attgagaaca gcagcagcag
    59881 agatgccgcg aggatcaccg aagcaatcgc gctcgctgcc gggtaatcaa actcctgtaa
    59941 gcgcacaaaa atcatcagcg acgtcacttc cgtcttccac gcgatatttc cggcgataaa
    60001 aatcaccgcg ccaaattcac caagactacg ggtaaacgac agcgtcacgc ccgccaccag
    60061 cgccggagaa agctccggca gcaccacttt gcagaaactc tgccagcgcg ttgcaccaag
    60121 cgtttccgcc gcttcttcat attccgggcc taactcttcc agcaccggct gcacggtacg
    60181 caccacaaac ggaatgctgg taaaggccat cgccaccgca ataccaagcc aggtataggt
    60241 gactttgata tcaaacttcg ccagccattc accgtaaaaa ccgtttacgg aaaagagcga
    60301 ggccagcgtt aaaccggcga ctgccgttgg cagcgcaaag ggtaaatcca tcagcgcatc
    60361 aagcagcgtg cggcccggaa agcgataacg tgttaaaatc cacgccatca gcagaccgaa
    60421 aacgccgtta aaaatcgatg ccacaaacgc cgacagcagc gtcactttgt aggccgcgac
    60481 cacctgcggg ttggtgatca cctcccagta ctgcgcccag ctcatctcag aaagctgcat
    60541 caccagcgca gagagcggca gcagcaatat caggcacaca aacagcagac tggtgccaag
    60601 gcttaaagta aagcccggca gcacgcgtct tgaagaaact gcaaacatta cttacgcccc
    60661 gccgctaaca gcttgtctaa ctcgccgccg ctggtgaagt gggttttcat cacttccggc
    60721 catgagccaa atttgtcttc cacgcggaac agttcggtct gcgggaattt atctttcagt
    60781 ttgtccatga cttccgggtt attcacacgg tagtaatagt cggtgatgat ggtctgcgcc
    60841 tgtgggctgt agagccagtt caggtaggct tttgcggctt tttccgtacc gttggcctgc
    60901 acatttttat cgacccacgc caccgggaat tccgccagaa tattggtttt cgggatcacc
    60961 acttcaaagc cctgtgcttc atactgttta cggatgttgt tcacttccga ttcaaagcta
    61021 atcagcacat cgcccaagcc acgctcggca aaagtggtgg ttgcgccacg accgccggta
    61081 tcgaacactt caacgttttt caggaactgg gtcataaact gttcggtttt ggctttatcg
    61141 ccaccgtcag ctttatccgc tgcgccccat gccgccagat aggtataacg cgcgttaccc
    61201 gacgttttcg ggttcgggaa aatcagcttc acgtcggagc gcaccaggtc gttccagtcg
    61261 tggatattct tcgggttgcc cttacgcacc aggaagccca tggtggagta gaacggcgag
    61321 ctattattcg gcaggcgcga ctgccagtca gccgggatca gcttgccttt gtcgtgcagg
    61381 atttgtacgt cggtcacctg gttataagtg acaacgtctg cttttaagcc ctgcaaaatc
    61441 gccagcgcct gttttgatga cccggcatga gattgtttta tcgtcagttt gtcgccgccg
    61501 ttatcttttg cccattgctg ttcaaacggc ggattcaggg cggcaaacag ctcgcgggag
    61561 acgtcataag aactgttcag cagttccgtt gcctgtacat ggcccgccag cagcagagaa
    61621 gcgaccagcg cgagtgagtt ctttttcagt aagttaacgg ccattgcgca cccttataaa
    61681 tttaatgact ttctaatagc catcatattt ataacggacg tgaaaggagt aacggtttta
    61741 tataccgttt ggtgatttgg aagttaaaaa gggaataaga acttggttca acaggcgtaa
    61801 ttgttgcagg cagtttggac acggacagcg cggagaaacc ggagcgtaca catagtacgt
    61861 gaggatttcg agcactgccc ggggccaaaa tgacaaatga aatagcctgg tgaacttagt
    61921 tcaagacgaa atcctcccca cacatgcagg gaggaaacag aggtgaatca gataccgacg
    61981 ctaaccgtct ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc ggttaaatag
    62041 ctggattcat ccgatgcgag gaaggccgcc agttcgccga cttccagcgg atcggcgagg
    62101 cgacacagcg ggattgcttt tgccatttca gtcagcaccg attctggatc ttccgggttc
    62161 gactggcggg caatgctttc cgccattggc gtgcggacgt atcccgggca gatggcgtta
    62221 acgcgaatac cggattgcgc gtactccacc gccagcgatt tagtcaggcc aacaatcgcc
    62281 gctttcgtta aggcatacgc cgtttcgcca ggatcggcca ccatatcacc agtgactgaa
    62341 gacatcatca caatgcgacc atctttgcgc gcaatcatct ccggcagcac cgccttcgtg
    62401 acgttccata cgcctttaat attgatatca atatggaaat cgcgatcttc atcgctcata
    62461 tcgaggaaac tgcccagacg acaaacgcct gcgttattca ccaggatatc aatgcgccct
    62521 tctttttcct tcgcgcgttt gatagctgcg gctaccgacg ccgggtcacg cacatcggcg
    62581 acaaccgccg tacagcgatg accacgacca cacagttcgt ccgctagctt ttcgatctca
    62641 ggggagatat ccagcaagat taggttcgcg ccatgacgtg caaaagttct ggcaattcct
    62701 tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag tttacccatt
    62761 ttaatgctct ccttgttaat acagagcgtt acactccatt ccctctgaaa atacaggata
    62821 aacaaaatat tgctgcggat cagctcacta attcaggaaa atctcaatcc agtcgctgct
    62881 ttatgcatca tttcaggcgt tgtgttagct cactgctgcg ctgaatcatt ttcaatgacg
    62941 ccacatcatt gatgttgcac aagaccaaca aacagtaaat cggtaacaga attttgcgcc
    63001 gtgcgggtag acatcgacga gctacgccat tcggtttcac cggaaacggt atcgagcgtg
    63061 aaatgcgcca gccgccgcag cggagagtca gccagagaag taatggcaat caccgttgcc
    63121 ccctgctttc gtgccgcttc tgcgcacaag acaatctctt ttttactgcc gctgtaagag
    63181 atagcgattt gtacatcgcc ttttttcaac gcctgggaaa cggtggcttg tacgtgcgtg
    63241 tcggcttcgc aggccacgcg atagccaatt ttcatcagtt tgaacgacag atcgcgcccc
    63301 accagcgccg atccgcccag gcccgttatc tggataaacg gtgctttact gatgacctca
    63361 atgatttttt gcagccgcgc gtagtcgaac aacgcacagg tttgctccag cgccagctct
    63421 ttttcgcgat tcagtttgcg ggcaatcact tccagcgaat catcactggt aatcgaactg
    63481 tgcaggtgca gcgccgtggc attcgttttc tcccggctgg cactgtattc gccaattaac
    63541 gccatacgca attcagtaaa tccttgcgcg ccgagcttct gggcaaattt cacaatgctc
    63601 gactggctga taccgagctg tttcgccatc tgccgtgaag agacggattt cagctcgctc
    63661 actctggccc gcagaaagtc agcaattttt tgctcgttcc cggtaaattc actttccgca
    63721 ttgcgtattt tcgtcaggta caacatcttc cactctcctg ggctggcagc aaaaattccg
    63781 cttaagttct tatgacgctc ttcacactct gcgagtgtag actcaatgat tcctttaagt
    63841 ctgtcaattc gggatatatg attccataca tctgcaatta tttgatcgta aatagtaagg
    63901 tcaccaccga tgcaactaga aaagatgatt actgaaggct cgaacaccgc ctcggctgaa
    63961 attgaccgcg tatcgacgct ggaaatgtgc cggattatca acgatgaaga taaaaccgta
    64021 ccgcctgccg ttgatcgcgt cctgccggat atcgccgcgg cgatcgatgt tatccacgcc
    64081 caggtcagcg gcggcggtcg tctgatttat ctcggtgcgg gaacatccgg tcgtctgggg
    64141 attctggatg ccagcgaatg tccgcccacc tacggcgtga aaccgggtct ggtggttggt
    64201 ttgattgctg gaggcgaata tgccattcag cacgcggtgg aaggagctga agatagccgg
    64261 gaaggtggtg ttaacgatct gaaaaatatt aatttaacgg cacaggatgt ggtggttggc
    64321 attgctgcca gcggtcgcac gccgtatgtg attgccggac tggaatacgc acgccagctc
    64381 ggctgccgca cagtgggaat ttcctgtaat ccggggagcg ccgtttcaac caccgctgag
    64441 tttgccatta caccgattgt aggtgccgaa gttgttaccg gttcttcgcg gatgaaagca
    64501 ggtacagcgc agaaactggt gctcaatatg ctttccaccg ggctgatgat taaatccggc
    64561 aaagtgttcg gcaacctgat ggtcgatgtg gtcgccacca acgaaaaact gcatgtgcgc
    64621 caggtcaata tcgttaaaaa cgccaccgga tgtaatgccg aacaagcgga agcggcgtta
    64681 attgcctgcg agcgcaactg taaaacggcc attgtgatgg tgctgaaaaa tctcgatgcc
    64741 gcagaagcta aaaaacgcct ggatcaacac ggcggcttta ttcgtcaggt tttagacaag
    64801 gaataaccca tggccaaaga gatcagcagt gaacttctga acaccattct tacccgtgtc
    64861 ggcggaccag gaaatatcgc cagttgtggt aactgtatga cgcgcctgtg tctgggtgta
    64921 catgacagtt cactggttga tcccaatatc aaaacgctgg aaggcgtgaa gggcgtcatt
    64981 ttgaccagcg accaggtgca ggtcgttttt gggcctggta aagcgcatcg tgccgcaaaa
    65041 gcgatgagcg agctgctggg cgatgcaccg gtacaggatg ccgcagaaat cgccgcccag
    65101 aacaaacgtc agttaaaagc caaacaaacc tccggcgtgc aacaatttct cgccaaattc
    65161 gccaccatct tcacgccgct gatccccggt tttattgccg ccggtctgct gctggggata
    65221 gcgacgttaa ttgccacggt gatgcacgtt ccggcagatg ctcagggcac gctacccgac
    65281 gcgctgaatt ttatgaaggt gttcagcaaa ggtttgttca ccttcctagt gattctggtg
    65341 ggctataacg ccgcccaggc atttggcggc acgggcgtca atggcgcaat tattgccgca
    65401 ctctttttgc tcggttataa ccccgctgca accaccggtt actacgccgg ttttcacgat
    65461 ttctttggtc tgcccatcga tccgcgcggc aatattatcg gcgtgctgat tgccgcgtgg
    65521 gcctgcgccc gcatttaagg catggtgcgc cgctttatgc cggacgatct cgacatgctg
    65581 ctgacctcgt taatcaccct gctgatcacc gctacgcttg cgtacctgat cattatgccg
    65641 ctgggtggct ggttattcga aggtatgtca tggctgttta tgcacctgaa cagtaatccg
    65701 ttcggttgtg cggttttagc cgggctgttc ctgatcgccg tggtatttgg cgtgcatcag
    65761 ggctttattc ctgtttacct cgcgttaatg gacagccagg gattcaacag cttatttccc
    65821 atcctttcaa tggcaggcgc gggccaggtg ggcgcggcgc tggcactcta ctggcgggcg
    65881 caaccgcaca gtgcgctacg cagtcaggta cgcggggcga ttattcccgg cctgctgggc
    65941 gttggtgaac cgctgattta cggtgtcacc ctgccccgca tgaagccgtt tgttaccgcc
    66001 tgtttaggcg gcgcggcggg cggtttgttt atcggattga tagcctggtg gggtctgccg
    66061 atgggcttaa acagcgcctt tgggccgtct ggtctggtgg cgctgccgct gatgacttcc
    66121 gcacaaggca tccttccggc aatggcggtt tatgctggcg ggattctggt ggcatgggtt
    66181 tgcgggttta ttttcaccac gctctttggc tgccgtaacg tcaatctgga ctgatattat
    66241 gaaacggaca atgctctatc tttctctgct ggctgttagt tgtagcgtca gcgccgcaaa
    66301 ataccctgtt ctgacagaag gtagttgatc aagtatatgc ggcgttaaag cagtaataaa
    66361 aaggccagtc agtaatgtta ctgcctggcc tttaatcaat gaatcagaaa cgcttacagc
    66421 gccatcaact tgtccagcga cggtgcgaaa taatagccgc cggttaccgg tttggtgaaa
    66481 cgcaacatcg catcacgttt gccatcggta tcgccaaaca tgctcagcag ttgctgctca
    66541 atgttatgca gacgcgcgca gtaggcgcag aagtacagac cgtgagtgcc actggcggtg
    66601 ccgtacggca ggctctggcg aacaatcttc agccctttgc catcttcttt cagatcaacg
    66661 cgggtgaggt gagaggtttc cggacgttcg tcgccgtcaa tctcttcgtt ggcctctttg
    66721 gtgcgcccga tcatcatctc ctgatcgtga acgctcatcc ggttgagctg cttcaggttg
    66781 tgctcccaac gctgcacaaa cacatagctg ccgccggaat ccacgccgtc tttgataacc
    66841 gccacttcgc ggcgcgtctc ttcacccgcc gggttttctg taccgtcaac aaagccgctc
    66901 aggtcacgct cttccaccca acggaagccg tggatctctt ctttcacttc aatgcagtca
    66961 ccaaaggctt ccatcgccgc ctgagcgaca gagaagttta cgtcgtgacg cagagaaaga
    67021 atgtggatca acacatcgaa ctgggtcgtc ggcgcaaggc ctttaccgta gcccggaaaa
    67081 tctttcagct cttctgcccc aacgccgccg ctcagagcgc gccaggtgtt gttaccaaag
    67141 gcaaccaccg caccaagatg cgcgtccggg aatttcgctt caaaagttgc cagtttgtcg
    67201 gcaaatgttt tactggccgc acgcagggcg tcaacttccc ctttcacgtt ggcttcgatc
    67261 caaatcgccg cgcggcaatg ttctggcaaa atgccactct gaacctgaga cattgttcct
    67321 cctgaaaata agaatgccac gcaatccgtg gcattgttga gcgctatttt acgtgttttt
    67381 tcgcgacggc ggtttgttca gacgcaaatt aacgccgcca gataatttta ctgactttcc
    67441 agtttttcag ggtatcgtcc gaaggcatta acccttcagg accacgccat tccccgctga
    67501 actggtagct aatatgttga ctgccttcgg ctttacattc gacggcacga ttatcatcac
    67561 catcagcttt ttggcaattg ccaaatgctt tgctgtaaag gtcgctaaac ggtgtaccga
    67621 ttttaacacc ggtgtcagca ggaatatcgc tatccagcac gtcaatgcga ctgatcgtgc
    67681 cttgatcgcc gttaatcacc atcgccacgt tgtcgccttt catcacttca aaaaagcgca
    67741 ccacgtttcc gttcgcggtt ttcattccgc tgcgcaggcg ataatcgcca tcaagcgcat
    67801 cagcaatggc ttgttcttgc agtggtgtgg acgccgttaa ttcacccacg ccctgctcgc
    67861 tcactttggt ggacgatcca aaccagttcc acgggttagc ggcagaccag ttaactgacg
    67921 acatcgtgga acagccggtc agcatcaacg gcatagcgca taacattaaa cgcagcgatt
    67981 tcatgtcact tcctttggtt attcaataac gttgcttgga gtgcaaattc accaaaaagt
    68041 gccgttattc tttttcttca ttaaaacaag cgcgtaaacg tcgattggtc agcagccaga
    68101 ttaacgccac aatatccgcc actaccagcg ccagaccgat accgctaacg gattcaccgt
    68161 tcagccacag ccacggttgc cagcaaagta aaaccacctg cgccaacagc aacagaaaat
    68221 agagcacatg ccaggtgcga ggaaacgaag cccgccgacc gctcaacaaa aacgccagca
    68281 ccgccggaat accgggaatc agccccagcc agaaattatc gtgatcggga taaaacagat
    68341 ttagcagcgc agtaccctgc tcacgcgacg caccggcaat gacaaacagc acccaggttc
    68401 gcgcctgaag caatagcaca agccagaaga gcaagggtaa acgcaggcgt ccgtgcgcat
    68461 cataatggac aggatggaac tcagtactct tcatcttcaa tcaaacgctt acccagactc
    68521 agcacgtcgg cgtgttcata tcccaggcgt tcatacattc cgagcaccat gtcgttatct
    68581 tccggcacgt tgatctgaat tttcgggcag ccgcgagcaa tcagcttttt ctccagccgg
    68641 ttaagcaacg cattggcaat tccacgccca cgaaactctg gatgcacgcc aagataataa
    68701 gcagacccgc gatgcccgtc ataaccgccc atcaccgttc cgaccacttc accgtttacc
    68761 tcagcgacca aaaacaaact gacgtcatgg ttcatcttac gctcgatgtc catttccgga
    68821 tcgttccacg gacgcagcaa gtcgcaacgc tcccaaaggg tgatgacctc ttcgaaatct
    68881 tcctggcgaa atacgcgtat ctccatggta ttcgttacct ttttgcgggt taaaaggctg
    68941 attatggcgt gaacggtcga attagccaat atctgacgaa aatcggttga aaaagtggca
    69001 taatggggag ttgtcaacta ttgaaatgaa aagtaaaaca attctcaaca gcaaaccgtc
    69061 gtaacggatt acgcgatacg atataacatc tggaacttta ttattacaac tcaggccgta
    69121 tgagcacttt taaaccacta aaaacactca cttcgcgccg ccaggtgctg aaagccggtt
    69181 tagctgccct gacgttgtca ggaatgtcgc aagccattgc caaagacgaa cctttaaaaa
    69241 ccagcaacgg acacagcaag ccgaaagcca aaaaatctgg cggcaaacgt gtcgttgttc
    69301 tcgatccagg ccacggcgga attgataccg gagcgatcgg acgcaacggt tcgaaagaaa
    69361 aacatgtggt gctggcgatt gctaaaaacg tccgttccat tttgcgtaat catgggattg
    69421 atgcgcgttt aacgcgttct ggcgatacgt ttatcccact ttacgatcgc gttgaaatcg
    69481 cccataaaca tggcgcagat ctgtttatgt caattcatgc cgatggcttt accaacccga
    69541 aagctgccgg tgcttcggta tttgccctct ctaaccgtgg ggcaagtagc gcaatggcga
    69601 aatacctgtc tgaacgcgaa aaccgcgccg atgaagttgc cggtaaaaag gcgactgaca
    69661 aggatcacct attgcagcaa gtactgtttg atctggtgca aaccgatact attaaaaaca
    69721 gcctgacgct tggctcgcat attctgaaaa agattaagcc ggtgcataaa ctgcacagcc
    69781 gcaacaccga acaagccgcg tttgtggtgt tgaaatcgcc gtcggttcct tcggtgctgg
    69841 tggaaacctc gtttatcacc aacccggaag aagagcggct gttaggcacg gcggcgtttc
    69901 gtcagaaaat cgccacagcg attgctgaag gcgtgatcag ttatttccac tggttcgaca
    69961 accagaaagc acattcgaaa aagcgataag ttatgaaacc cgacgcacac caggttaaac
    70021 agtttctgct caaccttcag gatacgattt gtcagcagct gaccgccgtc gatggcgcag
    70081 aatttgtcga agatagttgg cagcgcgaag ctggcggcgg cgggcgcagt cgggtgttgc
    70141 gtaatggtgg tgttttcgaa caggcgggcg tcaacttttc tcacgtccac ggtgaggcga
    70201 tgcctgcttc cgccaccgct catcgcccgg aacttgccgg gcgcagtttc gaggcgatgg
    70261 gcgtttcact ggtagtgcat ccgcataacc cgtatgttcc caccagccac gcgaatgtgc
    70321 gtttttttat tgccgaaaaa ccgggtgcag agcccgtttg gtggtttggc ggcggcttcg
    70381 atttaacccc tttctatggt tttgaagaag acgccattca ctggcaccgc accgcccgtg
    70441 acctgtgcca gccatttggt gaagacgttt atccccgtta caaaaagtgg tgcgacgatt
    70501 acttctacct caaacatcgc aacgaacagc gcggcattgg cgggctgttc tttgatgatc
    70561 tgaacacgcc agatttcgac cactgttttg cctttatgca ggcggtaggc aaaggctaca
    70621 ccgacgctta tttaccaatt gtagagcgac gtaaagcgat ggcctacggc gagcgcgagc
    70681 gcaattttca gctctaccgt cgcggtcgtt atgtcgagtt caatctggtc tgggatcgcg
    70741 gcacgctgtt tggcctgcaa actggcgggc gcaccgagtc gatcctgatg tcaatgccgc
    70801 cactggtacg ctgggagtat gattatcagc caaaagatgg cagcccagaa gcggcgttaa
    70861 gtgagtttat taaggtcagg gattgggtgt aactccctca cccccattcc cgcatccgct
    70921 gatgcaacgt caacgacggc ttctcggcaa acagctgctg gtaatccgtg gcaaattgcc
    70981 ccagatgcca gaatccccac tgcatggcgg cgtcttttac cgttgtgctt tgcgaccacg
    71041 ggcttatcag ttcgcggcgt acggcgttca ggcgaatgcg tttcagccac gcgtttgggc
    71101 caatgcctaa aatagcgtga aacgcgtttt gtagcgtgcg gcgactgaca tgcagttgat
    71161 tacacaaatc cagcaccgtc accggttcgg acatgttttc cagcacatat tcacgggcgc
    71221 gggaaagcaa ccgacggtaa ctctgatgac tgatgctttc cgccgtcacc attggttgcg
    71281 cttcttccag catcgccccc atcgccatta gcaaattatc ccccagcact tttcgcactg
    71341 caggctgatg gagattttcc gggttctcgc aaaacgtcgc cagcgcctgt tggacaaagc
    71401 cccacagcgc ggctttatgc tgctctttca cttccagcgc cgactggcta cgcagcatat
    71461 gtaatacccg atccggattg tgcaaaaagt tagcctgaca ggtgatgaca tcttcagaca
    71521 gcactacgcc caggatcgta taatcgtccg gcgtacttaa ttcaaactcg gttccaccgg
    71581 ggcgcgtcgc tatttctgcg cttcccagac attgcgaacc gataaatccc tgctcaccgc
    71641 gcgtcgccgg aatgccaaac cagaacgagt tcggccagac caggcacgac tgacgcagcg
    71701 ccagaccggt gtattcacga aaaacctgaa tatcatcgag tagaatttcc gtaaattcac
    71761 catgaaactt gcccggatgc agctgatcgt aaatctgctg ctaggcggta atcgtcaaag
    71821 catgttcata gacatccgtt gtctgtcgtt gatgaacatt atccacttcg accttcggcg
    71881 tgagcttcag gttttcgggt aagggttcat gataaagatg gtgcaaattg gctgtacggg
    71941 tctttttcat gatgttaatg ccgggtgttg taggacaccc ggcacctccg acaggttaat
    72001 ggggcttgag acgataacga ctactgcgtt tacgtaacgt cccggcagaa aagagctgtt
    72061 cgagcgcatt tctggctttt tccagcggcc agccaaagtg ggcggccact tctcctgccg
    72121 tcattccctg acatactgat gttaacagcg ccaacaactc gtcagcagat tccgcgacga
    72181 taactggcgc gtcgggttcc tttacgggtt gcttcggctg gcggttaaag ccagtaacca
    72241 gccactgggt gtcatcgtct ggccgaccga tctccttacg gctgatgaca cgcccggtac
    72301 gcatcgcggc agcacaacct gcgtccagcg ccgcacgaca cgccgccaga tcgccttcca
    72361 ccaccagcgt taagcgacca gggtcaagca cttcgtgact gagcagacga acgttagctg
    72421 ctttgagcat ggcatccgct gcatctatcg cggcgaccat gccgtccact tccagcaatc
    72481 ccagtgcatt gatcatcggc aacctccgtt acgcacgctg gattggatta cgcgcgattt
    72541 ccagcactgc atcggtaaag gcgttacagg ctgctttaca cgcggcctgg ctaccggtta
    72601 aaaatgctgc cgagtagttg gtttcagacg gtggcgggac gtaggtcacc agctgtacgt
    72661 cggcagattt caacgcggca tcaatgccgt aggtcgcttc cagcggcggt gccaccagat
    72721 acgccatcgg atcgccaagc gtgatcccgg cggttgatga aagataagaa ccggtacgcg
    72781 aaaccacatg cgccaggaag gcggtgtttt ctgcgtcatt ggcccactgg aaagctgcgc
    72841 cattttcaat atgtgcaacc atcgcatcca gaccggcacg cacttccgcc gggtttggcc
    72901 caccaagcat aatcagcact tcaccggcag tcggtgacgg gccgtgtgcc gctcccgcat
    72961 acagcgagcg accatatacc acttcgacca tcgcttgttt ggtcgcttcg tcagccgcaa
    73021 tgtaggttac gtcgtcagaa tcggcagaaa tcagcccgag gctacgaata tgcggcggca
    73081 attttagttc acgcgcaaat tcagcgttaa cggaggcaat cacccgcatg gcggttaccg
    73141 acggtcgaat caaatctaaa gctggcatga tgcctcctta tcgggtcatg ttgatgccgg
    73201 atgctttctg ctccagcata cgtttcgcca aatccacaat gacggcacag cagcacgaaa
    73261 gagaagcaaa agtcagctac ctggtacact aggcagcggg tcagcaaagg ggggcaaatg
    73321 cccctttttt catgtcagat aatcagatca ttacctcaat aaactaataa tcacatcgcg
    73381 gttacaacca gtttgatagt gatagctcaa acccagacgt ggcgctggct gcaagcaaaa
    73441 taagccgcaa aaatgcgacc ccatgtttac tagccgcaaa aatgcggtcc tatttgccag
    73501 aaccttttat tattcaattg gttagtgcaa gaaaaagcgt gattgctgtt caagaagtat
    73561 ccagaaaggt catgattttg acgctgaaaa aaaataggtg ggcatttttg cccactcact
    73621 agccgcaaat ttgcccactc actagccgca tttttgcggc ccaaagtaat aaatacagag
    73681 aaataataaa tacagatcag cgccaagatt tttcgtcttt tcggcggaat catgagacta
    73741 gtgcttacgt cctgataacc tccagagcag accttaactt ctgtgcagca ggactacgct
    73801 cattgccgct cggctaggtc aaatcctcac gataaagctg ttcggattgt gaccataatg
    73861 ttaaaggact ctttaaatca ttccttgacg aattaactag ctggcttaag cggaatagca
    73921 tgcaggataa aaacaaaacc agcttagcag tggggagagc ttcgcttcgc tcgtctcctg
    73981 gggccaatgc tcaatgaaac gacgtaaggc acggagcgct caccataggt agacacataa
    74041 acatctgaga gcgcaccttc tatcgcgcta cgcgctcaat aaaaaccaac gacaccgcca
    74101 cactgcaaga ccaatggttt ttatcgcgta tacagaccag acaggaaaca gcagcaaagc
    74161 agcgtatagc caggtgcaga cagcacactc acacgacgca cagcaggctt aagtgatcac
    74221 tcaggaaact ttatccccgc cgccgatcac cgcgaactat catgaccgcg ccaaattcga
    74281 aaggaatggc ccatagaacg cgatgaacac acataagcgc tacacttact taacgtcgcc
    74341 acagagcgat tgagagcgct taaaatcgat gcgatttcat taaatttttc tcactcgcct
    74401 gaatggtggc gatcaggact gatcgcaaca ttgccccgcc tcgatattga gcgcataata
    74461 gatacataca ttaagcggaa aagagataag tttattgtga aaatcagggg gttacatatc
    74521 atgcgcatat cagaacaaca acggcagatc ctcgactatc tggcaaaaaa cgaatcaatc
    74581 accgcacgac aagccgcaga tctcgtttat ggtggcaatg ttacccgcgt ccaggttgac
    74641 gccgcccgcc gctcactcat gacgatggtt agtaatgggc taatcaggaa acaaggccgc
    74701 gctttcgtag ctgaaagaca tatcaatcac gcttatagcc aggacgttga agaattgcgc
    74761 cgccagatcc tttgggtatt cagcaaacag ggtcaggagt atttcctttc tgaacaccca
    74821 aatttcgccg ccaaagagtt aacagtaagc ggattgtcgt gcgcactgga ttatcttgtc
    74881 ggtgaaggtt gcgttactta caagcagcta tggcacgccc tgaatacgct tgtagaagat
    74941 ggtttactca ttgccacgcg tgagaacatc gaatactgga taatgccgcg ttcaggtcgc
    75001 cgcaatcatg cacggtatat ttcaggcaac agatacagaa cgccggactt taatccgaca
    75061 caggaagatc gggaccgtgc cgaaatgatg tgcagggaac aggagaaaag aattatggat
    75121 aagttatttt cgatgcagtg aacacaagcg ggcgcaatgc ccgtttttat tgccttattc
    75181 cacgcctgac gaatcaattt gatcacacct gagaaaatgc atagccagaa gttaaggccg
    75241 ttatattgcg attgggagcg ttttaaacgg tatcaattta attaattctg atgtggtgat
    75301 ttgcaggcaa aaaaaacccg ccggagaagc gggaaataga gacatatcgt tgtagttatt
    75361 aacagatcac tttgaggtcg agggcggcta accctcgcta atattatccc gctattacct
    75421 tgcgggtgca aataatcttt tattgctttg atgatttaat aaatagcact ttttgctaac
    75481 aaaaactgct gtcaatatat ggccaaaaat gtcaacagaa aaaataaggt ttataatatt
    75541 aagtatcaca tgggtaatgg gttcagggaa tcggaatcca ccgcctcacg gaaattcaaa
    75601 atttgcttct tctttcctcc gtgggcggct tctttttcgt atcgaggtat ttatgaaagc
    75661 aataacatta gaacagttcg cagcaatggc gaatgtatcg accagaacgc tgaggcgctg
    75721 gctaaaaaaa ccatcaaccg gagtcccttt gccatttacc ccacgcggta ctcgccaatt
    75781 attctgggag cacgaatgca aagaatggtt gtacagaaca gaacgataat aatcacgcat
    75841 cacattttga ggtaaacatc atgaccaata tcgaaagatt agaacgctgt gccgatatta
    75901 tgcggcgcag atggatttat gaccctgaag cgggtttatt gtttagccgt gaaacaaaag
    75961 acgttatcaa aggcagcatg accaataaag ggcatttggt tgtaacggta catgaaggta
    76021 aattcaaagt gacactcacc tatcagaaag cctgctatgt atacacatac ggcgcttatg
    76081 acgagacatt atatgaagtg caccacgtta atttgaacaa acaggataac cgcatcaaga
    76141 atattcgcct gatgccgaaa gaaaagcacc gccggataca taaaaacgtc cgcaaactta
    76201 ttcggctggg ccacacacaa tggcttcaac cgcaattaat tgaacaatca ctataaacac
    76261 aggagtaaat accatgcatt cattagacag ttattttcag cgcacgacag cccctaaatc
    76321 agccgcccag gaacgccgtg aggaattcca tgagaaagtg atgcgttctg ctgattatat
    76381 tgcggacaag ttcgtggaaa cggttcgtcc acttgttgat gaagtagctg ataaacttca
    76441 gtctgaaatg ccggaagata tggaaggcac tgcaaagcgg cgactcattt gcgaattaag
    76501 tcgtcgtttt ggcgtgtcaa taagcgcttt taaataatcg aaaaaagggg ggaaagataa
    76561 tggacgcatt tgaggtactg gcaaataaag gcgctgaact ggtagcacag cgtgataaaa
    76621 ccgccaacga aggggaacgc gcactgctaa ataaacagat taaagcgatc cgtatggccc
    76681 agtttaaatt aatcagtaat gaagttatcg aaacaattaa accgcagatc ccacaaatcg
    76741 ttgctgacgc tattaaagcc gccggactgg agaaacgcat tgccgggcta aaaacgaacg
    76801 cagagaaaat ggagatgcac aaacagagcg gtgctccgaa tcccgatgag tacttctcac
    76861 cggatgttga gtttatggcg caggttgagg aacgtctggg tagctgtctc accgaggaac
    76921 agcgtcgcta ttttgatgga gtggatagtt cggcaggtat tgacctgaac tcgtatttcg
    76981 gtcgtgaaat tgagcatttt gatgccgccc cgtctatgcc ggagccagaa ccagaaatgg
    77041 ccaccgatcc tgaagctgag caacggaaac gcgtattcaa tgcgataggg attcggtact
    77101 gatcggataa aagaaagccg ggaattaccc cggctgactt aacaatgtga ccaatgaaca
    77161 aatcggacaa gacaaacagg ataagcagtc tcgaaacaat gttcgggaac tatattagca
    77221 caagtccgga gaaaatcatc atgttggcac tacgcatcat gcagggcatc gctaagaccc
    77281 tcgcagaaca tgtcttagac ctgaaacatt cgccactgag caaacaggcc atgaagcgcc
    77341 aaacgcttcg tctgtgggct gaatattcgc ttggaactat caataaaatc atcgacatga
    77401 aatcggggcc gagcaaccag agcgcggagg aaatggagtt catcaggcgt ttaattctca
    77461 ttcgcagaga cattcatagc cagttacaca gcgtgggaat cgatattaat gatgggacgg
    77521 gcgattaatt ttcggacaag tactatgcgc gcgcgaggag ctgaacatga agcaagatat
    77581 tgcagacagg ctggagatac tcgaaggcca gcgggcagaa gcgaaacaac tacgtaagca
    77641 ggccagaaga gcgcacagga acaacgaagc agagttacta accaaataca tcagtttcac
    77701 caattactgc atctacgagt gttacaagga agatgcagag gactggctgg attcattgcc
    77761 agaacagtac tgagcagaat aacaccacgt catgttgttc tgaggtgagc atatcacctc
    77821 tgttcaggtg gccaaattta gtgtgccact tcagcatcac cgttgaaatc acaataacga
    77881 tttttttgta aatatgctct cacatatgta ggaatagacc aggttggcat tgaaacacat
    77941 ctaccatgac cataataaag taattttttg atatgtttat ggttttatat taattaaaat
    78001 atatctaaaa ttgatagttt taaaattgca aaatccagat tgtgtttgta atgtttaatc
    78061 tgattgtatt gagtggcgtt tattatgaaa aataaatcag gtaattcttg ttatagagaa
    78121 gcagtcgata ttcatagctc acttgagctt tcatttatat tgcataaata tcatttgcgt
    78181 gtagatgaga agaaatctcg aaatgtattc agcgccatac gtgatgagct catccttgat
    78241 ggggattcac gacaaaactt agctactttc tgtcaaacat ggcttgatga tgaaatccgt
    78301 gagttgctta atttagctat tgataaaaac atgatttata aagatgaata tccacaaatg
    78361 gcggaacctg aaggtcgctg tgtgcgtatg ctttccgatt cgtggagttt ccccgattcg
    78421 cgacacacct taggttgctc aaccattggt tcatctgaag ctgctatgct cggccgcttg
    78481 gcgttaaaat ggcagtggtg taaaaaacgc gaagtacaag gtaaatcaac cgaacctgat
    78541 ttgcgatcct gtgcaaatat gctggcataa attctgccgt tactgtgatg tgaaattgtg
    78601 tgaaattcca cttgatgatc atcacctgat aatgaatgct aaagaggttt tgaagcatgt
    78661 cgatgaaaac actattggtg tcgttacgac tttaggtatt acttattttt gtaaagcaga
    78721 ttgcgttagt gtttttaaga taagtgttaa agttaatatt agtttttata tttacatcgt
    78781 aattatatgt ttctatttta aaaatgttcg agagtataaa aatgtctaat gctaatatcg
    78841 cacccaaaag taagaaaatg acagtgggga cactcgctat catgaatata actgctgtag
    78901 taagtctacg atttatacct tctgaagcag aatacggctt aggtgccatt ttttactata
    78961 cctttgctgc aattatgttt ttagtacctg ttgcctttgt tgcggctgag ctagctacaa
    79021 catatccaca aaaagggggg gcatttcgat ggataggaga agcatttggg gctcgctggg
    79081 gttttgtagg aatgcttatg acctggctac aagtcatccc ctactttcca acagtactga
    79141 catttggtgc agtctcggtt gcatttattg atgttgatat gggggtggca gaatcaattg
    79201 ccagtaataa atggtatatt ttatttttcg tactatttgt ttactgggga gctatttgtg
    79261 ttgcattacg tggcgttggt attttctcgc aggtatctaa gtggtgcggt attataggga
    79321 caattattcc agctattatc cttgttattc ttggtttctc ctatctgtgg ttcagcggta
    79381 aaccagcggt gatcccatta ggttggggtg atcttttccc taatatagct aacttccaga
    79441 atattgttct tgccgccagc atatttcttg cttatgcagg gatggaaatg aatgcggtcc
    79501 atgttaacga tttagataat ccaactaaaa aatacccaat tgcaattacc atcgcttcta
    79561 taggtactat tgcaatattc ctgctcagta ctttaggggt cgcctttatt attcctacag
    79621 ataaaattag tctaactcaa agtttgttgg ttgcctacga tatgctgttc gaatgggctg
    79681 gagtgccatg gcttggctct gtgatggcat ttatgctagc aattggcgta ctgggtgggg
    79741 tggttacttg gattgcaggg cctaatacag gtgtattagc gatcgcgaaa gctggttatt
    79801 tacctaagtt tttccaaaaa accaatcgtc atggaatggg gcaccatcta atgtttgtac
    79861 aaggtataat tgtctcagtt ctttctgtta cttttgtgat aatgccttca gttcaagcag
    79921 cttttcagat tttatcacag ttaacagtta tgctctattt agttatgtat atgctcatgt
    79981 tcgctagtgc tatctatcta cgacattctc aacctgatac aaaacgccca tatcgagccc
    80041 ctgctttatt tttctgggca acacttggat tcttagggtc ttttctggct tttgtattaa
    80101 gttttattcc gcccgagcaa atcccaactg gtagtccaac cagctatgtt ctttatttgt
    80161 tagtattagt tgccctattt ttattaattc caatattaat ttacgccttc cgaaaatctg
    80221 actggaaaga tccaaactct gatttcgaac cattttcttg ggaaaaaaga actagtaatt
    80281 aaattttcca taagctacaa aagatgaggt taaatatgtt tagtgctgaa tctccaattg
    80341 gtgtaataga ctcaggtgta ggtggattga catcggtaaa ggaaataatt aatttgcttc
    80401 ctggtgaaga tattatctat tgtggagata atatgaatgc accttatggc aaccgttctg
    80461 ccgatgatat catcagtttg actaaaaaaa tgctgacttt tttacagtca aggaatgtca
    80521 aactagttgc tgtcgcatgt aatactatat cctcaacttt ggaatccgaa gaatacagcg
    80581 gatatgctaa gagctttccg tttcctatct tatccatcat tgaacctgca gtagaagatg
    80641 tgattcgcca gcaatataaa aatgttggta ttatcgctac cgagtttact attaaaacgg
    80701 ggtgtcacaa agagctaata aaaaaattga attcaacaat aaatgtattt ggagaaccaa
    80761 gcaaaaatct tgcaatgtta attgaggaag gtaatcttaa tgctcctgca atcttgaatg
    80821 acatcaaaaa acatgttaac catttaattt ccctccatcc agtaaatgaa attattctcg
    80881 gctgtactca ctatccaatt gttcagaatt tttttgaagc agttgcaccg gatataaaat
    80941 ttattaatcc tgcacatgat caggctatct ctattaaaaa tcacttggga cagctaaatt
    81001 tattaaactc ctcaaatata ggaactttgc aaataaatac tagtggaagt atggagatct
    81061 acaaaacggt actaggggaa ttgagcatta cgaaacctca tacattcagt atcaggcagt
    81121 tttaaattta tccggtgcac cgtaaaatcc cgtccttctg aactgcaccc caaaagttgg
    81181 acaccaactg agtaaggtgc attttatgga caaacccagt tacatactgg gtagtgcatc
    81241 caattagtag aacatgtgtt tttcgataaa cgctccgatc actttttcgt ggatctcaac
    81301 tgagcgtgag aagcagattg ttttacgacc caaccgctta atgcgggtgc gtagcgtcag
    81361 attattacgc tcaatgcgtt gggtgaatat tttgccggtc agatgcttat tcttcggcac
    81421 ctcccggcca tagctgcccc agtcatcgct ggtcagcatg ccgatgttga agggtgtaag
    81481 cagtgccagt agctcccggc acgtttgatc ggttcgggga ccaaaagtgt aggccagtac
    81541 accgcctgtt ttggtgttgt acgcgtaccc gagccaatgt tgccgggctt tactgccaac
    81601 gtagctccat tgctcatcaa gctcgcagat aaacgccaca tcagcatggg caacaggcga
    81661 agaagttatt cgctttggcg cgagtttttt aaagtccgga tgacggtgtt actaccaatt
    81721 ttcagtgtcc tggcggtatc gcgaaccccg gcaccattaa aggccatttc agttatcagc
    81781 tctttcatac ccggcttacg tgcttgataa gtataagtga actgaaacac acggtggcag
    81841 tcacggcagc gaagtctgtc acggccttta gggttctgac catggcggta aacctgagct
    81901 gactgacaac ggggacaatg aatattaacg ctggtcatga gataacctca aaagcccgta
    81961 ttatacatca gattcaacta attagaggca tcacccatcc agcattataa aacatactaa
    82021 caataactaa attataataa tcacaactta caatcaaata ataaatgcat aagattaaca
    82081 atgatttttt cgggttatat ttatctaaaa aagcagaata ttatgtaact tattattttc
    82141 taagcgaaaa tttattgtgc ggtcagacta acaaacttgc gtaccacccg aagaacatgc
    82201 gaagatttaa gtattctttc ttttttggtt tttatttttc attttctctt ttcttctctt
    82261 tccggttctg ttctggtttt ggttttttcc tttttatttt tattctttct tcagggttat
    82321 gctaaaacac caaaacattt ttcaccacaa aagcatccaa tcatcttgcc agaccaatca
    82381 ccttaaccgg atacactgcc ggatataatc ccactaacac ccagacacca agcaccatac
    82441 ctgataacta ccgtattcag gaaggaaatg cgagtgattt caccgtcacc ggcacaaccg
    82501 atccgccaaa aagaggcgta ccaatgtcga tatagtcccc cgcgcggaca atcacttcgt
    82561 caatgactgc caacgggagt tgttgtagct gtgggcgcaa caacatgccc agagctttac
    82621 caaagtcctg cccggccacc accagcaggg gatgcggatt cggaaaacgc gcgacgaaat
    82681 cgaccagcgc gttgatgacc gtcagaaccg cggcgtaacg cacaggcagc gaggcgggaa
    82741 gcgccagcac gtacacgtca gttttgggat caagatccag ctgaagcagc gcctgttgcc
    82801 aggcactcac cagatccgtt tcatcaatcg ggatcgccac cggcaaattg cgcagcggca
    82861 gttgtacgcc ctccagccag attgtgctgc cagagagcga aagggtatgt gcacccgcgc
    82921 caatcaccgt ggagcgtacg gtttgcgccg gaaactgcac attcatctca cgcaggcgcg
    82981 gatggtcatg cagcgccgtt gccagaagtg ggccaatatc ggcaaaacag aacgggttgg
    83041 cgggctggtg gcgataacat tcgcccacgc ctccagaaag cgtaatgatt tcgggcgtaa
    83101 cacctgcggg cagcaaaccg gtttgcatca atgcctgcgc gagcggcgaa agcgttccgt
    83161 caatcacttc gacaatcagc gccgccatcc gccgggtaac ctgcaccagc tgcgcgccgg
    83221 tcagcgaacg ggcgtcagtg cctgcaccga agcactcatc cacaatcatc tgccccggtt
    83281 tatgagcgta aaccacgcgc ccctggctgt cggtttccag caggcgacca ccgacgttga
    83341 gacaggcagt gccgctgatt tttccggcat cgaacagggc gtagttcgcg gtgccaccgc
    83401 cgatatcgat attcagtacc cgacacagcc gttgttcaga aagggtttgc gccccggctc
    83461 cgtgaccggc gatcacggat tcgaggtgcg gcccggcgct ggcgacgaca aaatcgccca
    83521 gcgactgttt ttccttcatc actttttcca tcagctgtgc gaccaggctt tcggtaaact
    83581 ggccttccgg cagctgggca atgatggttt cgcggatgcg ctggctttcg gttttgtcgg
    83641 ctggcacgct ggcgggcacg gacgccgtta ccgggatcga ttcatcacat tcggtgatgg
    83701 taaaacccag tagatccgcc acttcacggg cttccggggt aataatgctg gcgcgcagaa
    83761 ccacggacat tgcctgttcg ccgcgcgcgt gtgcttcacg aatatcattc gctgtgatga
    83821 gttttttcac ctgcttcctc ctgtttggtg agagatgcca gataatccac cagttgctgc
    83881 acgctttgcg ggtcgtggct gtttagctcg aaaatcggct cttcgaaacc cgtctcaagc
    83941 aataactttc gcgttgcggc gacgtcagca tctggcatgt ccgttttact gatgacggcg
    84001 atttgccgtt tgctgacgcc aatatccagc aacccggcag gcaaccgact ttccgggtca
    84061 tttgcgccat ggacatagat cagcatgtct acatcctgta gcgtggtaat taaggcgtga
    84121 taccagcggg gatggctaaa atattcaccc ggcgtatcaa tatcgccctt atcattaaat
    84181 tccacggcca gtgtttttct ggcgagggta taattcccct gcaacgcatt aaataacgtt
    84241 gtttttcctg ccccgacgct acccacaaac gcaattcgtt tcatgaatgc ctcgtattaa
    84301 cttttggtca tttcacaaag cgtataattt aataagcgac ctaaaccgct gacggtttgt
    84361 gataacgctt cttctactgc gcccaccgag ccataaatca ccaatgcacc gctgaagcgg
    84421 tcgagaaaac cgatatgcac atcagcggct ttcagcgcta aatcaccggc gatcatcgcc
    84481 gtttcgccgg gagttagcgt cataatgcca attgcgcccg catccggaac gccgattttc
    84541 ttcgccagtt cctcaccggg atgcgcgatg agatgcgcca gtgtgacctg tttgccgggc
    84601 acaaattcct gaatgatgcg ttctttatcc atgagtcgcc gcctgtgaaa aactttcgtg
    84661 tatcgtgaca atttttcgtc agagtaaata acaaaattcg gcaagcgcgt ttaaaaggtg
    84721 aggtagatca ataagaatgg agagatattt actgttatga ataaactttc atatgcgacg
    84781 catgaatata aataaaataa tatcgagata ttttatatac tgattttttt attattcgca
    84841 aactcagtaa taagctttgc taatatattt caggaaaacc gcttaccctg aatattcagg
    84901 gtaagcgtga gagttaagaa tgaattacag cggttgggtt tgcgcttcaa ccacggccag
    84961 cgccaccata ttgacgatac gacgcaccga tgcgatcggc gttaacacgt gaaccggttt
    85021 cgccacgccc atcagcaccg gaccgacagt cacaccttcc gagctggaaa cgcgcagtaa
    85081 gttgtaacta atgcgggcag cttccatgtt cggcatcacc agaatattgg cagaaccttt
    85141 caaagggctg tccggcatac ggtcgttgcg aatcgcttcc accagcgctg catcgccgtg
    85201 catttcacca tcaatcatca gatctggcgc acgatccctg accagttcca gcgcctgacg
    85261 cattttgctc gacgacgggc agtcagaaga accaaagttg gagtgcgaca acagagcaac
    85321 gcgcggctca ataccaaaac gacggacagt ttctgccgcc atcaaggtga tctccgccag
    85381 ctcttctgcg tctggttcgt cattgacgta ggtatcggca ataaaggtgt taccactcgg
    85441 cagcagcagc gcgttcattg cacctgcggt gtgaacgcca tcgcgataac caaagacatt
    85501 tttcaccacg ctaaaatgtt catgataatc gcccaccgta ccgcagatca ttgcatcggc
    85561 ttccccacgc tgaaccatga tcgcgccgat cactgtcggg ttactgatca gcgcacgctg
    85621 cgcctgttcc tgagtgacac cgcgacgctt catgatctgg aagtattcgg tccagtactc
    85681 tttaaagcgc ggatcggatt cgttattgac gatttcaaaa tcaacgcccg ctttgatctg
    85741 caagcccagt ttctgaatgc gcatttcgat cacgttcgga cgaccgataa ggatcggttt
    85801 cgccagtccc agcgttacca gttcctgagt cgcatgcaga acgcgcgcct cttccccttc
    85861 cggcagaact acgcgcttcg gcgctttgcg agcctgggag aaaatcggct tcataaacag
    85921 gttggttttg taaacgaact cagtcagctt gtcgatgtag acgtcgaaat cagcaatcgg
    85981 acgcgtcgcc acgcccgact ccatcgccgc ttttgcgacc gcaggagcga tcttaacgat
    86041 caagcgcgga tcaaacggtt ttggaatgat gtattccgga ccaaagctca gatcctgaac
    86101 gccatacgct gaagccacca cttcactctg ttctgcatgg gccagttctg caatcgcgcg
    86161 taccgccgcc agtttcatct cttcgttgat ggcggttgcg ccaacgtcca gcgcgccacg
    86221 gaagatgaac gggaagcaaa gaacgttgtt cacctggttc gggtagtcgg aacggccggt
    86281 acagatgatg gcatccggac gcacttcttt cgccagcggc ggcagaattt ccggttccgg
    86341 gttcgccagc gccaggatca ttggtgcccg agccattttc ttcaccattt cctgggtcag
    86401 cactttcggg ccggaacagc ccaggaaaat atccgcacct tcaatcacat catcgagggt
    86461 acgtttgccg tcatccacca ccgcatatgc ggctttggtt tccgccatat ttggctcacg
    86521 gccctgatag ataacgcctt ttgaatcgca aaccacgatg ttatgttttt gcagccccag
    86581 cgctaccagc aggttcatac aggcgattgc tgcggcaccc gcgccggaaa ccaccatccg
    86641 cacgtcggag atgtttttct ccaccacgcg caagccgttg aggatggcgg cagtgctgat
    86701 gattgccgtg ccgtgctgat cgtcgtggaa taccggaata ttcatccgct cgcgcagttt
    86761 ctgttcaata tagaaacatt ctggcgcttt aatgtcttcg aggttgatgc cgccgaaggt
    86821 cggttcgagc gcggcgacga cttcaataaa tttgtccggg tcgagttcat caacttcaat
    86881 gtcaaataca tcaatcccag cgaatttctt aaatagaacg cccttgcctt ccatcaccgg
    86941 tttgcctgcc agcgcgccaa tgttgcctaa ccccagcacc gccgtaccgt tagagatcac
    87001 cgcaaccagg ttaccgcgcg cggtatattt gtaggctttt agcgggtctt tttcgatttc
    87061 aagacaaggt gcggcaacgc ctggtgagta ggccagcgcc agatcgcgct gcgttgccag
    87121 aggcttggtt ggagaaacct ggattttccc tggaactgga aattcatgga aatcaagtgc
    87181 actttgtttt aactggtcat ccatttggtt gttcctttca cgtaacgttc acaaataaag
    87241 tgtgtgggca acagcccctg cccacaacgt ggcgcacatt attaccctgc cggagtctac
    87301 agactttgag caagtccaaa ctctcaccat taatataatg ttttggtaat aatcctataa
    87361 cactgatgtt acctgcttaa tccagcaata ccatgcctgt ctgctatgct tttttgatgc
    87421 gtttagcgaa atttctcaga agtgtgaatt aacgcactca tctaacactt tacttttcaa
    87481 ggagtatttc ctatgaacga gttagacggc atcaaacagt tcaccactgt cgtggcagac
    87541 agctgcgata ttgagtccat tcgccattat catccccagg atgccaccac taatccttcg
    87601 ctgttactca aggctgccgg attatcacaa tatgagcatt taatagacga tgctatcgcc
    87661 tggggtaaaa aaaatggcaa gacccaggaa caacaggtgg tcgcagcgtg tgacaaactg
    87721 gcggtcaatt tcggtgctga aattctcaaa atcgtacccg gttgcgtgtc aacagaagtt
    87781 gatgcacgcc tctcttttga taaagaaaag agtattgaga aggcgcgcca tctggtggac
    87841 ttgtatcagc aacaaggcgt tgagaaatca cgcattttga tcaaactggc ttcgacctgg
    87901 gaaggaattc gcgcggccga agagctggaa aaagaaggta ttaactgcaa cctgacgctg
    87961 ctgttttctt ttacccaggc gcgggcctgt gcggaagcag gcgtttttct gatttcgccg
    88021 tttgtagggc gtatttatga ctggtatcag gcacgcaagc cgatggaccc gtatgtggtg
    88081 gaagaagatc cgggcgttaa atcggtgcgc aatatctacg actactataa gcaacaccac
    88141 tatgaaacca ttgtgatggg cgcgagcttc cgtcgcaccg aacaaatcct cgccttaacc
    88201 ggctgcgatc gactgactat cgcaccgaat ttgctgaagg agctgcagga aaaagtttct
    88261 ccagtggtac gtaaattaat cccgccttct cagacgttcc cacgcccagc acccatgagc
    88321 gaagcggagt tccgttggga gcacaatcag gatgcgatgg cggtagaaaa actgtctgaa
    88381 ggcattcgtc tgttcgctgt tgatcaacgc aaactggaag atcttcttgc cgccaaacta
    88441 taaaccagcc acggagtgtt atatgtcccg aaaagacctt gccaatgcga ttcgcgcact
    88501 cagtatggat gcggtacaaa aagccaattc tggtcatccc ggcgcgccga tgggcatggc
    88561 tgatattgcc gaagtgctgt ggaacgattt tcttaaacat aatcctaccg acccaacctg
    88621 gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg tcgatgctgc tctacagttt
    88681 gctacatctg accggttacg acctgccgct ggaagaactg aagaacttcc gtcagttgca
    88741 ttcgaaaacc ccaggccacc cggagattgg ctatacgcca ggcgttgaaa ccaccaccgg
    88801 cccgcttgga caaggtttgg cgaacgccgt cgggctggcg atagcagagc gtacactggc
    88861 ggcgcagttt aaccagccag accatgagat cgtcgatcac ttcacctatg tgtttatggg
    88921 cgacggctgc ctgatggaag gtatttccca cgaagtctgt tcgctggcag gcacgctggg
    88981 actaggcaag ctgattggtt tttacgatca caacggtatt tccatcgacg gtgaaacaga
    89041 aggctggttt accgacgata cggcaaaacg ttttgaagcc tatcactggc atgtgatcca
    89101 tgaaatcgac ggtcacgatc cgcaggcggt gaagaaagcg atccttgaag cgcaaagcgt
    89161 gaaagataag ccgtcgctga ttatctgccg tacggtgatt ggctttggtt cgccgaataa
    89221 agcaggtaag gaagaggcgc acggcgcacc actgggggaa gaagaagtgg cgctggcacg
    89281 gcaaaaactg ggctggcacc atccgccatt tgagatccct aaagagattt atcacgcctg
    89341 ggatgcccgt gaaaaaggcg aaaaagcgca gcagagctgg aatgagaagt ttgccgccta
    89401 taaaaaggct catccgcaac tggcagaaga gtttccccga cggatgagcg gtggtttacc
    89461 gaaggactgg gagaaaacga ctcagaaata tatcaatgag ttacaggcaa atccggcgaa
    89521 aatcgctacc cgtaaggctt cgcaaaatac gcttaacgct tacgggccga tgctgcctga
    89581 gttgctcggc ggttcggcgg atctggctcc cagcaacctg accatctgga aaggttctgt
    89641 ttcgctgaag gaagatccgg cgggcaacta cattcactac ggggtgcgtg aatttggcat
    89701 gaccgctatc gccaacggca tcgcgcacca cggcggcttt gtgccgtata ccgcgacgtt
    89761 cctgatgttt gttgaatacg cccgtaacgc cgcgcggatg gcggcactga tgaaagcgcg
    89821 gcagattatg gtttataccc acgactcaat tggcctgggc gaagatggtc cgacgcacca
    89881 ggctgttgag caactggcca gcctgcgctt aacgccaaat ttcagcacct ggcgaccgtg
    89941 cgatcagatg gaagcggcgg tgggctggaa gctggcggtt gagcgccaca acggaccgac
    90001 ggcactgatc ctctcaaggc agaatctggc ccaggtggaa cgtacgccgg atcaggttaa
    90061 agagattgct cgtggcggtt atgtgctgaa agacagcggc ggtaagccag atattattct
    90121 gattgccacc ggttcagaga tggaaattac cctgcaagcg gcagagaaat tagcaggaga
    90181 aggtcgcaat gtacgcgtag tttccctgcc ctcgaccgat attttcgacg cccaggatga
    90241 ggaatatcgg gagtcggtgt tgccttctaa cgttgcggct cgcgtggcgg tggaagcagg
    90301 tattgccgat tactggtaca agtatgttgg tctgaaaggg gcaattgtcg ggatgacggg
    90361 ttacggggaa tctgctccgg cggataagct gttcccgttc tttggcttta ccgccgagaa
    90421 tattgtggca aaagcgcata aggtgctggg agtgaaaggt gcctgatggt gatcgccgga
    90481 tgctgtatgt tcccctcacc ctaaccctct ccccagaagg gcgaggggac ggtccgtgcg
    90541 ctttttaggg ccgcatctgc gattttgttt aacgggtgat ccacaacgtg ggcctggcgt
    90601 ctggcccatg ccagttatcg caggtaggtt ctgcggcgta acgcaccagg cgaaaacgct
    90661 gaccgtcaaa gcgccagcgc gcctgaatgc cacaatcgct taatccgcgc cctttcgcta
    90721 aggtcaccag ttcacgcgat ttctcatcaa atgttgcgtt catcagttcc agttcattcg
    90781 tctcctggcc gttgttgaac ggcaaacgca accgaaccgg gcgcgaggct agcggctttt
    90841 tacgcgacac aatccatgcc aaatcaatgg tgttataggc ccctgcctca cagctaatca
    90901 tcatcagtgc tttatcatcg gtcagcgcag tgacattcac ctcacgacgt aacggatcaa
    90961 gcgagcagcg cagaccattc atccgccagt ttccataatc cagcaaatcg ttacgttctt
    91021 cgagtgagag tggcgtcggc gttgggttaa ccaccgcgac ctctttcagc gcaggcgcgg
    91081 gcggtacgct gagaggcggt tcgtcccctt tcttgatcca cgcggtttca ctgccaacgc
    91141 gcttttgctg agcatcaata aacaacaacg ccgcttttaa gccgctcaga gaaatggtct
    91201 gattgccgtc gcgtaaggtg atcgccttcc cttcctgaat tatctgcaaa aacgcggtga
    91261 tggttgccgt atcatcggtt actaataacc atggtgaaat ccgccacttg tcaccactta
    91321 atgccaacgg ctcaccatct aatagcagcc gtggcgctat ctccccttca gacgcatccg
    91381 gcgacttcaa cccaccgcgc tcaatacgta aaacggcatc ggtatgcgcc ccagcgctgc
    91441 ggctcagggt catcaccagt ccattatgat cgcccgtatt gcgcgccaca caaaaatttt
    91501 gattattaca ggtgacctgc cagtcggaaa acgcccgttg cgctggtgcc gcccacacca
    91561 aagacgtcgg caacaaagcg aaaaaaaaga gaaggaaaat gcgatagcgc atggacggta
    91621 cgaccccaga agttcaaaca aacagcacaa gtcgtatctt cgttgcgcag gtagcgcggc
    91681 tcaatcggat ttgtcggatt gagccaataa taaacaacga aatattattg ctatttttca
    91741 gtccattaaa tgtgacattt gcaaatagtt aagcaataac accgtcttac cgtcacgtat
    91801 ctcgccggtt ttgatcatct ccagcgcctg gctgaacggc agctcgagca cttcaatatc
    91861 ttcatcttca acaccgccac cggcgttagc gcgctgattg tcactgtatt cggcgataaa
    91921 aaagtggatt agctcagtca caccgccagg cgacatatac agttcaaata atttgcgcac
    91981 ttcgcccact tcatagcccg tctcttcaat cgcttcttta cgaatgcaca cttccggttc
    92041 gtcgttatcc agcagcccgg cgcaggtttc aatcagctgc ccgctttcat tgccattaac
    92101 ccaggtagcg acacggaact gacgaatcag aaccacgctc tttttcttcg cgttgtacag
    92161 gaggatcgtc gcaccattac cgcgatcgta aacttcacgt ttatggcgga taacttcgcc
    92221 atctttgcgc gtgagatcgt aagtaatgtt gtgcagggtg aaatagttat cggagagaat
    92281 tttgtcttta atgagggtga tttgttgcgt cataccgact ccacagcgcg aaatgaacaa
    92341 ttatattacg ctgtgaagtc gggtttgtct gcgcaggcta tcagtgagat ttgactgatt
    92401 ttacccccag ccagtcaata atcccctgtg ccgcatgacg tccttctgcc attgcggtaa
    92461 ccaccagatc cgcaccacgc acggcgtcgc caccggcgaa gattttcgga ttggtggtct
    92521 ggtaacggta ctggctttcc acatccgcga tgatgcggcc ccatttgtct accgttacac
    92581 cgtgcgactc cagccacggc atcccgtgtg gattgaagcc aaacgccata atcaccgcat
    92641 cggctggcat gacaaattca ctgccctcaa tcggtacagg tcgccgacgc ccctgggcat
    92701 ccggctcgcc aagacgcgtg cgcaggaaac gaattccgca aacgtgacct tgctcattca
    92761 gctcaagcgc caccggctgg acgttaaatt cgaagttggc cccctcttcg cgggcgttct
    92821 tcacctcttt cttcgagcct ggcatgttag cttcatcacg acgataagcg caggtgacgt
    92881 tactcgcgcc gtggcgcagc gcagtacgca cacagtccat cgcggtgtcg ccgccgccca
    92941 gtaccacgac gttaagtccg gcggtattga taaacggctc ttccggtagc tcttcgagcc
    93001 ccatcacctg tttagtgttg gcaatgagga acggcagcgc gtcataaacg cccggcgcat
    93061 cttcattggg taaacccgct ttcatggaac ggtaagtgcc tacgccaacg aagaccgcgt
    93121 cgtattgttc caaaagcaaa tccaaagaga catctttacc cacttcacaa ttgagttcga
    93181 agtgaatccc catcgcgctg aagatttccc gacggcgtgc cagcagggat ttatccagtt
    93241 tgaaagaagg aatgccgaaa gtgagcaagc caccgatttc tggatggcga tcgtacaccg
    93301 tcaccgccac gccattgcgg gtcagaacat ccgcacaggc cagccctgcc ggacctgccc
    93361 cgataatcgc cacccgcttg tccactttgg tgacatgact taagtcagga cgccaacctt
    93421 tcgccaacgc ctgatctgaa atatagcgtt caatgttgcc gatagttacc gcgccgtgct
    93481 catcgcgaat agtacaggca ccttcacaca aacggtcttg cggacaaacg cgtccggtaa
    93541 tttccggcag ggtgttggtc tggtgagaaa gctcgacggc ggcgtcgatg tttccggctt
    93601 tcaccagttc aatccactgc ggaatatggt tatgcagcgg gcaggtccat tcacaaacgc
    93661 tgtgctcgcc gaacttaagg cagcgcgagg cttcccgttg tgcctggtcg gcgcgaaatg
    93721 gcagataaat ttcatcaaaa ccggttttgc gcgcttcaat cgccagttta tccggctcgc
    93781 cacgcgcggg cgttgcctgc atttgttcga ctttactcat taccggcatt tcttgtgcag
    93841 cggtactggc atgccacggt tgatgctcct gacgtgcggt gcgcaagcgg cgggatttcg
    93901 ccatgccgga gagtgcgacg tcagtgacca gttgcagcgc gtccgccggg caattctcga
    93961 cacaggcagg accgttttcg cgccctgcac aaaggtcgca tttatgcgcc gtggctttta
    94021 cttttcctgc cgcgacgggt gtcaggacga tttgcatcgt accaaacgga caggccacca
    94081 cgcaggattt acagccaata cacttttgct gattgacctg aatgctgtca tcaacgtggc
    94141 tgattgcgcc attagggcag ctacgggcgc agggcgcatc ttcacaatgg tgacaggtca
    94201 ctgcactacg ttgctgctga tgtttgataa ccgtaattcg gggatgaaaa tggtgttggc
    94261 tcaggacatg ttgctcatca ttgtgagcca tgacacaggc gatttcacaa gcatgacaac
    94321 ccagacattg ctgactgttg gccataataa aacgattcat aacgaccttc ttttttggtt
    94381 gtaaaaacct tattctttat atgagtgttg ttattacccg acttacaggg gaaccggcaa
    94441 tgttcatgtg cccagaataa gtaactattt cgctataaac tgtggcagat caaataatcc
    94501 catcactgac taaattgcgt ttcagcgaac tggaacatta atgatttttt gtggagaaga
    94561 cgcgtgtgat tgttaaacga cccgtctcgg ccagtctggc ccgggccttt ttttacattg
    94621 tgctgctgtc gattctttcc acgggtatcg ctctgctaac tctggcgagc agtttgcgcg
    94681 acgctgaggc tatcaatatt gccggatcgc tgcgtatgca gagttaccgc ctgggctacg
    94741 acttgcaaag tggcagtcca caactcaatg cacatcgcca gttgtttcag caggcactgc
    94801 attcaccggt attaaccaac ctcaacgtct ggtatgtgcc agaagcagta aaaactcgct
    94861 atgcgcatct gaatgccaac tggctggaga tgaataatcg gctcagcaag ggcgatttgc
    94921 cgtggtatca ggccaatatt aataattatg ttaatcagat agacctgttc gtgctggctt
    94981 tacagcacta cgctgaacgc aaaatgctgc tggtggtggc gatttccctg gctggcggta
    95041 tcggtatttt cacgctggtc ttttttactt tgcgccgcat acgccatcag gtggttgccc
    95101 cgctgaatca gctggttacc gccagtcagc gtattgaaca cgggcagttc gactcgccgc
    95161 cgctggatac cagtctgccg aatgagcttg gtctgcttgc aaaaaccttt aaccaaatgt
    95221 cgagcgagct gcataaattg tacctttcgc tggaggcgtc agtagaagaa aagacccgcg
    95281 atctccacga gaccaagcgt cgtctggagg tgttgtatca gtgcagtgtt cccaggcgct
    95341 gaacaccagc cagattgatg tgcattgttt ccgccatatt ttgcagattg ttcgcgacaa
    95401 tgaagcagct gaatatctgg agctgaacgt tggggataac tggcggatta gcgaagggaa
    95461 gccaaaccct gagctgccaa tgcagatttt accggtgaca atgcaagaga cggtttacgg
    95521 cgaactgcac tggcaaaata gtcacgtttc atcatcagaa ccgctgctta acagtgtttc
    95581 atcgatgctg ggacgcggtt tgtactttaa tcaggcgcag aagcattttc agcagttatt
    95641 gttgatggaa gaacgtgcga ccatcgcccg cgaattgcac gactcgctgg ctcaggtgct
    95701 ttcttactta cgtatccagt tgacgttact gaagcgttcg ataccggaag ataacgccac
    95761 cgcacaaagt atcatggccg atttttccca ggcgttgaat gatgcttatc ggcagttacg
    95821 cgagctgttg actactttcc gcctgacgct gcagcaggcg gatctcccct ccgcgttgag
    95881 ggaaatgctg gatacgttac aaaatcaaac cagcgctaaa ctgaccctcg actgccgtct
    95941 gccaaccctg gcactggatg cgcaaatgca ggtgcatttg ttgcaaatta ttcgcgaagc
    96001 ggtgctgaat gcgatgaagc acgccaacgc cagcgaaatc gccgtcagtt gcgtcaccgc
    96061 gccggacggc aatcacacgg tttatatccg tgataacggg attggtatcg gtgaaccgaa
    96121 agaacccgaa ggtcattatg gactgaatat catgcgcgaa cgcgcggaac ggctgggcgg
    96181 gacgctgact ttttcacaac cttccggcgg cggcacgtta gtgagtatta gctttcgctc
    96241 cgcagagggt gaggggaagg ggaacacgcc ggctgaggtg taaataatta ccctcacacc
    96301 ggccctctcc ctttaaaggc gagagggaaa aacgtgccca tattgacctg taagcgagtg
    96361 tgtcagcaat ctcatgataa tttacattaa ctcctttttt tctccacgat tggctcgtac
    96421 cttgccgcta cagtgaagca agtcaagcct acaacaatac gcagaaacac gaggtcctct
    96481 tttaatggcg aatttcttta ttgatcgccc catttttgcc tgggtgctgg caatcctgtt
    96541 gtgtctgaca ggtaccctag cgattttttc attgcccgtt gaacaatacc ccgatctcgc
    96601 gccaccgaat gtgcgagtga ccgctaacta tcccggcgca tcggcccaga cgctggaaaa
    96661 caccgtgacc caggttatcg agcaaaatat gaccggcctc gataatctga tgtatatgtc
    96721 atctcagagc agtggcaccg gtcaggcatc tgtcacttta agttttaaag caggcaccga
    96781 tccggacgaa gccgtgcagc aagtacaaaa ccagctgcaa tcagccatgc gaaagttacc
    96841 gcaggcggtg caaaatcagg gcgtgacggt gcgtaaaacc ggcgatacca acattctgac
    96901 cattgccttc gtctctaccg atggctcgat ggataaacag gatattgctg attatgttgc
    96961 cagtaatatt caggacccgt taagccgcgt gaatggcgtc ggggatatcg atgcctatgg
    97021 ttcgcaatat tccatgcgta tctggctgga cccggcgaaa ctcaacagtt tccagatgac
    97081 ggctaaagat gtcactgatg ccattgagtc acagaacgcg cagattgcgg ttgggcaact
    97141 tggtggtaca ccttccgtcg ataagcaggc gctcaacgcc accattaacg cccagtcact
    97201 gctgcaaaca ccagaacagt tccgcgatat caccttgcgg gtgaatcagg acggctcaga
    97261 ggtaaggctg ggcgatgtcg ccaccgtcga aatgggggcg gagaaatacg attatcttag
    97321 ccgcttcaat ggtaagccag cctccgggtt gggggtaaaa ctggcctcca gcgcaaacga
    97381 aatggcgaca gcggagctgg tgctcaatcg tctcgacgag ctggcgcagt atttcccgca
    97441 cggactggaa tacaaggtgg cgtatgaaac cacctcgttt gttaaagcct ccattgaaga
    97501 cgtggtgaaa acgctgctgg aagctatcgc tctggttttc ctcgttatgt atctgttcct
    97561 gcaaaacttc cgcgccacgc tgatacccac tatcgccgtg ccggtggtgt tgatgggaac
    97621 cttctccgta ctttacgcct tcggttacag cgtcaacacc ttaaccatgt tcgcgatggt
    97681 gctggcgatc ggtctgctgg tggatgacgc catcgtggtg gtggaaaacg tcgaacgtat
    97741 tatgagtgag gaaggactca ctcctcgcga agccacacgt aaatcgatgg ggcagatcca
    97801 gggggcactg gtcgggattg cgatggtgct ttcggcggta tttgtaccaa tggccttctt
    97861 cggcggcacc accggtgcca tctatcgcca gttctctatt accattgttg cggcgatggt
    97921 gctgtcagta ctggtagcga tgctcctcac tccggctctg tgtgccacgc tacttaagcc
    97981 actgaaaaaa ggtgagcatc acgggcaaaa aggttttttt gcctggttta accagatgtt
    98041 taaccgcaac gccgaacgct acgaaaaagg ggtggcgaaa attctccacc gtagcctgcg
    98101 ctggattgtg atttatgtcc tgctgcttgg cggcatggtg ttcctgttcc tgcgtttgcc
    98161 gacgtcgttc ttaccgctgg aagaccgggg catgtttact acctcggtac aattgcccag
    98221 cggttcaacc caacaacaga ccctgaaagt cgttgagcaa atcgagaaat attacttcac
    98281 ccatgaaaaa gacaacatca tgtcggtgtt tgccaccgtt ggttctggcc ctgggggtaa
    98341 cgggcaaaac gtggcgagaa tgtttatccg cctgaaagac tggagcgaac gcgacagtaa
    98401 gaccggcacc tcgtttgcca ttatcgagcg tgcaacgaag gcgtttaacc aaattaaaga
    98461 agctcgcgtt atcgccagca gcccaccagc aattagcggt cttggtagtt ctgcaggttt
    98521 tgatatggag ttgcaggacc acgctggagc gggtcacgat gcactgatgg cagcacgtaa
    98581 tcagttgctg gcgctggcgg cggaaaaccc ggagctaacc cgtgtgcgcc ataacggcct
    98641 cgatgacagt ccgcagttgc agattgatat cgaccagcgt aaagctcagg cgctgggcgt
    98701 tgatatcgac gatattaacg acacactgca aaccgcctgg ggttcgagct atgtgaatga
    98761 ctttatggat cgcggtcgcg tgaagaaagt ctatgtgcag gcagctgcgc cgtatcgcat
    98821 gctgccagat gacatcaatc tctggtatgt ccgaaataaa gatggcggca tggtgccctt
    98881 ctctgctttc gcgacctcac gctgggaaac aggctcgccg cgtctggaac gctataacgg
    98941 ttattctgcg gttgagattg ttggggaagc cgcaccgggg gtcagtaccg gtacggcgat
    99001 ggatattatg gaatcgttag tgaagcagct gccaaacggc tttggtctgg agtggacggc
    99061 gatgtcgtat caggagcggc tttccggcgc gcaggctccg gcgctgtacg ccatttcctt
    99121 gctggtggta ttcctgtgtc tggcggcatt gtatgaaagc tggtcggtac cgttctcggt
    99181 aatgctggtc gtgccgctgg gggtaatcgg cgcgctgctg gcaacctgga tgcgcgggct
    99241 ggaaaacgac gtttacttcc aggtgggcct gttaacggtc attggtttat cggcgaaaaa
    99301 cgccatcctg atcgtcgagt ttgctaacga gatgaaccaa aaaggccatg acctgtttga
    99361 agcgacgctc cacgcctgcc gtcagcgttt acgcccgatt ctgatgacct cgctggcatt
    99421 tatcttcggc gtattgccaa tggcaaccag cacgggtgcc ggttccggtg gtcagcatgc
    99481 ggtgggtact ggcgtaatgg gcgggatgat ttcagctact attctggcta tttacttcgt
    99541 gccgctgttc tttgtgctgg tgcgccgccg cttcccgctg aagccgcgcc cggaataagc
    99601 aataaaaaag gcgacatgcc aatgtgtcgc ctttttcaac tttccgatta agaacctgct
    99661 cagcgggttc ttgctgttta tactttgtct caggaattac ttacgaagca taacttcgat
    99721 aaagtctttc cagttcccca gttcacgttc aatcataaca acctctctta taattatggg
    99781 tattctacgg aaacaatata ccgtggtgaa gctaatttac tcgattgctg cgatgactac
    99841 ctccggggga caaaccttat gtaaatacta tggtcctgcg gtgataattt gtatgtgata
    99901 cacagcaaca tttcgagata ttcatacggc atctaatact gatttaattc tggttaaaat
    99961 acagacagat aacaagatga atattcttaa tgtttacgtt aaaaatgttt aatattattt
   100021 aatagttgtt aatttgaata cttcgataat gttatatttt ctgataatca tttgcaggca
   100081 aaatgttttc acccttaaat gagtatttat tctcataaat cgaaaaagga ttcattatgg
   100141 ttacacttta cggcatcaaa aattgtgaca ccattaaaaa ggctcgccgt tggctggaag
   100201 ccaataacat cgactatcgt tttcatgatt accgcgtcga tgggctggac agcgaattat
   100261 tgaacggttt tatcaacgaa ttaggctggg aagcgttact caacacccgt ggtacaacct
   100321 ggcgtaaact ggacgaaact acccgcaata aaatcaccga tgcggcctct gcggcggcat
   100381 taatgactga aatgcctgca attatcaaac gtccattgct ctgcgcgcct ggtaagccta
   100441 tgctgctggg tttcagtgat tccagttatc agcaattttt ccatgaggtg tagtctatgt
   100501 cgtgcccggt tattgagctg acacaacagc ttattcgccg cccttccctg agtcctgatg
   100561 acgcaggatg tcaggctttg ttgattgaac gtttgcaggc gatcggtttt accgttgaac
   100621 gcatggattt tgccgatacg cagaattttt gggcatggcg tgggcaaggt gaaacgttag
   100681 cctttgccgg gcataccgac gtggtgccgc ctggcgacgc cgatcgttgg atcaatccgc
   100741 cgtttgaacc caccattcgt gacggcatgt tattcgggcg cggtgcggca gatatgaaag
   100801 gctcgctggc ggcgatggtg gtagcagcag aacgttttgt cgcacaacat cccaaccata
   100861 cggggcgact ggcatttctg atcacttctg atgaagaagc cagtgcccac aacggtacgg
   100921 taaaagtcgt cgaggcgtta atggcacgta atgagcgtct cgattactgc ctggtcggcg
   100981 aaccgtcgag tatcgaagtg gtaggtgatg tggtgaaaaa tggtcgtcgc ggatcgttaa
   101041 cctgcaacct caccattcat ggcgttcagg ggcatgttgc ctacccacat ctggctgaca
   101101 atccggtaca tcgcgcagca cctttcctta atgaattagt ggctattgag tgggatcagg
   101161 gcaatgaatt cttcccggcg accagtatgc agattgccaa tattcaggcg ggaacgggca
   101221 gtaacaatgt tattccgggt gaactgtttg tgcagtttaa cttccgcttc agcaccgaac
   101281 tgactgatga gatgatcaaa gcgcaggtac ttgccctgct tgaaaaacat caactgcgct
   101341 atacggtgga ttggtggctt tccgggcagc catttttgac cgcgcgcggt aaactggtgg
   101401 atgcggtcgt taacgcggtt gagcactata atgaaattaa accgcagcta ctgaccacag
   101461 gcggaacgtc cgacgggcgc tttattgctc gcatgggggc gcaggtggtg gaactcgggc
   101521 cggtcaatgc cactattcat aaaattaatg aatgtgtgaa cgctgccgac ctgcagctac
   101581 ttgcccgtat gtatcaacgt atcatggaac agctcgtcgc gtgatgagtg gttctgcaag
   101641 aggaaataag catggactgg ctggctaaat attggtggat tctggtgatt gtctttttgg
   101701 taggcgtcct gctgaacgtg attaaagatc tcaagcgcgt cgaccataag aaatttctcg
   101761 ccaacaagcc ggagcttccc ccgcatcgtg atttcaacga taagtgggac gatgacgacg
   101821 actggccgaa aaaggatcaa ccgaagaagt aagctgagtg ctcaaccgta ggccggataa
   101881 ggcgttaacg ccgcatccgg caatggtgaa cgatccctga tgcgacgctc acgcgtctta
   101941 tcaggccagg taatctgtct tttacatcat ttcaatcaca tcatcatcgc caggcttacc
   102001 gccacttagc gcttggtcga aataatgttt cggaatggta taacgcaagt gatccagggc
   102061 aaattgcata ctgcgattgt cgatcgcatg gcctaaatcc tccacgatat ccagcgtcac
   102121 gtcaccaccg gcactgatta acgcttcctg cgccgcaact gcatgcgcca gatcgataac
   102181 cggatcttca ccaccatgaa tcaagtgaat tgtcgtggct gtagacgcgg tctccggcag
   102241 gctcgcatag cgtccgttaa aggcaatgac gcgcgaagca aggcccggtt cggctttaat
   102301 gctctccagc gccataatcg cgccttgtga gaaaccaatt agcgccgtgg cgttagcccc
   102361 tactccgctc tgtttctgcc agtagcgtac cgtttcaata aacgtcggca tgatggcatc
   102421 cacacgcgcc tggcgattat cttccgtaat accctgcact gaaaaccact gacgccccgc
   102481 cggattaccg ctcggctccg cgccaccgac actcaccacc agcgcatcag gaaacagcgg
   102541 tgcaaaccag ctgcctattt cccccattgc caccgggtta tccccgacac cgtggaaaag
   102601 cagtaacaac tgttgtgcag gtttatccgg gctttgaaca acaaaatggt catgtttcat
   102661 ggcgaactcc ttaactgatg tcattaattt tacgccgctg actcacaatg accatgggag
   102721 aaaattgaag gagtcagtga aaaaattgcc attgcgttat gcgatcgcgc agacgctcag
   102781 tgcgaacctc atttagtgcg aacagcgcct gcgcggcctc ttcccgctgg cgaaccagta
   102841 acatcttgcg gcctgaaagt ttaagtgtgg tacataactg cgcgtcactg gcgtttttct
   102901 gtaaacgccc acgcagcgcc ggtaatgcca gttcactggt ttgtagcaga cgcaataagc
   102961 aacctaacga tgttaatagc ggacgatgag cgaaagcaaa accggccagt tcaagccagt
   103021 cgtcgtcaga aaggatggca tcgtttagtg gatcaacagg aagcatttcg ccattccact
   103081 gcgcgagagc ttgcgcatcg cgacgtaaac ggtaatgctc acgttcagcc agctgtttac
   103141 ccgcatcact catcggtaac agcgccatcg ccgtatagca accgctgctg gcttcccgat
   103201 gattacccat ccgcaccagc acaaaaccgc agcgttgcca gaaacgccat aactccccag
   103261 tgtaaccaaa actcaccgaa agatagtcga ggtcatgcgt atattgcaaa gctccagcaa
   103321 taagttgtcg ccctgtgcct tcccgctgac gagccggatg aactgctatc cggctaaccc
   103381 gccttccgcg caatgtcgcc gccagtggat tgctgccgtg cgccgccagc gactgggcca
   103441 ccagattacc ccgcgggcga cgaaaacctg cccataccgc ctgactgagt tcttgagata
   103501 atccgccctc atccaccagc cacaacgccc cggcaatctc gttttcgcca gccgcctgta
   103561 aaaaatgttg ccctggtgca tccatcatcc ggcgtaaatc cagcggcgag gtccggtagt
   103621 gcgcaccaga caataactga taaaccttta acggcgtttc tggctcgctt cgccataacg
   103681 tttgttcaaa tgcggaaatg acgatattgc cttgtggtgt atgggtgaag ttttcatcgt
   103741 taaaaaccag tgcctcacta accatttttt ccagcgggca tccctgtgcc cagcggatcg
   103801 gctgttgcag ttcaaaacgg tgtaaatgcg gaaagcgagc gcaaaatttc agcaaaaaac
   103861 cacgtccggt accttcgtag ccctgcaccg tagtggttaa caacgttcga ggaaaacgcg
   103921 ataccagttg atgcaacaac ggcgcaggta tggctgcggc ttcgtcgacc accagccagt
   103981 cggcttgctc atcgctggct aacaaggcat ccggtgcaat aaagcgaaac ttctcgcccg
   104041 caaattgtgc cagtacatcc gttgccgctt ttgcgggcgc ggtgacaatc gcactgcccg
   104101 caatacgaga aatgagttgc cctgccaacg ccgacttacc gcgcccacgc gcagccgtta
   104161 ccgctgccac gcccggcggc atggtcagta gctgctgtaa gagttgctgt tgttctggtt
   104221 gtggtgcgcc ggtcgcgggg tgccagtcag tacggggagt aaaatgcgcc aacgagaacg
   104281 gctggttttg ccgccagagg atagcgtcgt tatccgccgt aagtacgcgt ttgagatgct
   104341 ggacaaaatg cggcgtcgca atagggtcag ggcaatcact ccagcgcagc gagtcggcat
   104401 caggttggtt ttcccactct tcccatacag ggagtaacaa aaccagccag cttcccgctt
   104461 tcaacgttcc gctaagtgct gcaaaggcag cggcatcaaa gccgtggcgg gcgtcgaata
   104521 ccgcatgccg gaactcgcgc ccaagtaaag tttgtagcgc cgagggtgaa cagtggtttt
   104581 cagcatctag ctgcggcgaa atccacagcc agtcgccagg taaggcatca cgcaacttaa
   104641 gagcatgatc aaaacaccaa ccctcttccc cgctcaacac cagcaagcgg cagatccctt
   104701 cacgtttcat ttgcgctgtt aatgtgtaaa gcgcagtcag ttcagccatc cctgccccgg
   104761 aaattaaatg cttttaccaa aagtattgca ttgtgccgga tcgccgctgt cgaaaccacg
   104821 tttaaaccag ctgtagcgtt gctgagaagt gccatgtgtg aaactgtctg gtactactcg
   104881 cccctgactt tgctgttgta aacggtcatc gccgatggcc tgcgccgcat tcagcgcctc
   104941 ttccagatcg ccagtttcca gaacgccttg ctgctgcata ctatgccccc aaacaccggc
   105001 aaaacagtcg gcctggagtt ccatacgcac agataagcgg tttacttccg cctgcgtcgc
   105061 gttttgttgc agttgacgaa ctttcggctc gatgcctaac agtttctgca catgatgacc
   105121 gacttcatgg gcgataacgt acccctgggc aaaatcgcca tccgcgccaa gtttgtcttt
   105181 catgtcatca tagaaggaga gatcgatata aactgtgcca tccgccgggc aatagaacgg
   105241 ccccataatg gactggcccg ccccgcagcc ggtgcgcgtc attccacggt acatgaccag
   105301 cttcggttgc tgataggtct tacccatctt ttcgaactgt tgtccccagg tgtcttccgt
   105361 ggttgccaga atcaccgagg tgaattttgc ggcttcgtct tcattagggc taattgaccg
   105421 cgttgattgt tgttgagaaa ccggctgccc ggtcatcaac ccggttaaat caacgccata
   105481 gtagcctgca accagcacga ctatcagtaa aatcagcccg cctttaccgc tcggcagacg
   105541 gaaaccggga ccgcccattg acggaccgcc agagctgttg cgcctgtctt caacattgtc
   105601 actttcacgt cgcccttgcc aacgcataga tacctcaaca atatattcat tatagaaatg
   105661 atcgtaggtg gttcagagga agattaccac aggaaagcgg gatgaaatgg cggaggaatg
   105721 agcgttcaat gcatatattc cgagagcggt tgtcttgccg tgccagctgc acggcaagat
   105781 gatgaacaga aaaatcagtc cagctgtaca cccaggcggc gggcgacggc ttcataggct
   105841 tcgatcaggc caccgaggct ctggcggaaa cggtctttgt ccattttctc cagtgtttct
   105901 ttgtcccaca ggcggctgcc gtccggggag aactcatcgc ccagtaccac ttcacctttg
   105961 tacagaccaa actccagctt gaagtcgacc agaatcagac cagcatcatc gaacagtttt
   106021 ttcagcacgt cgttcgcttt gtaggtcagc tctttcatac gcgccaggtt ctctttgctc
   106081 acccagccaa aggtttcgca gtaagattcg ttgaccatcg gatcgtgcat ggcatcgttt
   106141 ttcaggaaca gatcgaacag cggcgggttc agctcaatac cttcttcgat tccaagacgt
   106201 ttcaccagag agccagcagc acggttacgc acgacacact caaccggcac catatccagc
   106261 tttttcacca gacattcggt atcggagagc agacgctcca tttgagtcgg gatacccgct
   106321 tcagccagtt tgctcataat gaagtagttg aacttgttgt tcaccatacc tttgcgatca
   106381 aactgctcaa tgcgcgcgcc atcccctgct gacgtatcat tgcggaattc gagcaccaac
   106441 aggtccgggt tttccgtgct gtatacggtt ttcgctttac cacgatacaa ctcagcttgc
   106501 ttttgcatct ttatcactcc tgggtgtgaa ttaacgtttt aaaatctttt gctgtctggt
   106561 gtgccggatg ttttgtcgga tgcggcgtga acgccttatc cgaactacgt ccgacaaaca
   106621 aatttcgtgc gaattaccgc taaaatcgcg ttttcctgcc gacgcacacg tttgcgtatc
   106681 atatcagaaa aaagggccgg atgattccag ccctgtattt ttacttgcta aacgcagcct
   106741 ggaagacagc taccagcgcg tcgttctgac tctgagtcag agtatgacct ttcggatcga
   106801 tgaactgtag gctgctgcgg ttatctaaat cgccaacctg cagtttatag tcaccggatg
   106861 ccaggcctgg atcgctcgcg cccagttcct gccagtcgct gtcagacagc ggcttataag
   106921 ttacggccat gttgccctgc gaacgggtgc tgtcggtcac tttcatgccc actttttcca
   106981 gcgccgctgg cagacgttgc caaaccacat tgaacagccc gcgtacgacc agcattggta
   107041 aaccggtgtc atcagctgca ctttgtacgt ccatagtggt ggaggcacga ttttgcgcag
   107101 cgttcgcggc gtcagtggca gatttatcca gaccggcgga aataacgttc atcatctccg
   107161 tgctgtaacg ctgcatggaa gccgcgtctg caaccggttt gcccgcctgt tccaggttca
   107221 gcagtttaac cgtaaccgcc tgctgataac cctgcggctt aacagagatt tgataacgac
   107281 cacgatactg ctcgtcttcg tccagacggt tccattgtac ccaatcggtg gtcagtgtct
   107341 gaccagcatc atcacgttgg gtgatggtgt agtttttcgc ctgcagcacg ctaaccacct
   107401 gcggccacag agtattgcca cgaccatttt ccaccagcaa tgaagcggta tcgcccgtga
   107461 actgggtacg cgcgccagaa accagtgcca acggctgggc tggtggacga atgtccagcg
   107521 ccttaccgac agcaccacta ccgttggtca ccgggattgc ataatcaccg gaggtcaccg
   107581 gcaaaatcat tccagccggg gcatgaagct ccgcaagcgg tgccgcttcc aggtaggctt
   107641 catcaccact gacctgacgc ttatagcgtg agtcagaact acaggcagcg agtaataaaa
   107701 caagcgaaac acccgcaacc tttgccaggc gcgacttttg aacagagtaa gccatcaaat
   107761 ctccctaaac tttacagcaa accggcatgc ttaagcgccg ctctgaccgt ctcacgacca
   107821 ctgtcggtga ttggtgtcat tggcaggcgc agcgtatcgg tcgccacaag acccagttcc
   107881 ttacatgccc atttcaccgg gattggattg ggttcgacaa atagtttgtt gtgtaatggc
   107941 atcagacgct gattaataac gcgtgcctcg gcaaaatgcc cttctgctgc cagtttgcac
   108001 atctgggcca tatcacgcgc tgcgacgtta gccgtaacgg aaataactcc atgaccgccc
   108061 aattgcatga agtccagcgc gctcgcatca tcgccgctca gcagaacaaa atcatctgaa
   108121 accagctctt tgatctggtt tacacgcgtt aagttccctg ttgcctcttt gattccgata
   108181 atatttttta ctttcgccag acggcccacc gtttccggga gcagatcgca gccagtacgg
   108241 gacggcacat tatacagaat ttgcggcagg tcagtatgct cagcgatggc tttgaaatgc
   108301 tgatacaaac cttcttgcga cggacgattg tagtaagggg ttaccgtcag gcagccgacg
   108361 ataccactgt cattgaagcg ctgcgtcagg ctaatggctt ccgcagtagc gttagcgccg
   108421 gtcccggcaa ttaccggaat gcgcccatca gccagatcca gcgtcatcat caccacatca
   108481 gcatgttcgt catgatttaa ggtagcggac tcgccagtgg tgccaacaga aacgatcgcc
   108541 gaagtaccgc tggcgacatg ataatcaatc agttttttca agctagcccg acagacatta
   108601 cctttttcat ccatcggagt aacaatcgcg acaatacttc ccgtgaacat gggccatcct
   108661 ctgtgcaaac aagtgtctca atggtacgtt tggtatggca ttaaaagcaa gcagacagaa
   108721 ccgttctgat tgttgtatgc atgatttttt tatgctttcc ttaagaacaa ctcacccctt
   108781 aaaggaataa ccagtttgac actgtcatcg caacattatc tggtgatcac tgcgttgggt
   108841 gccgatcgcc ctggaattgt gaacaccatc acccgtcatg tcagtagttg cggctgtaat
   108901 attgaagaca gtcgcctggc gatgctggga gaagagttca cgtttatcat gctgctttcc
   108961 ggttcatgga atgccatcac tctgattgaa tcaacgttac cgttgaaagg tgccgaactg
   109021 gatcttttaa tcgtgatgaa gcgcacgacg gcgcgtccgc gtccgccaat gccagcatct
   109081 gtctgggttc aggtcgatgt tgcagactcc ccgcatttaa ttgaacgctt cacagcactt
   109141 ttcgacgcgc atcatatgaa cattgcggag ctggtgtcgc gcacacaacc tgctgaaaat
   109201 gaacgggctg cgcagttgca tattcagata accgcccaca gccccgcatc tgcggacgca
   109261 gcaaatattg agcaagcgtt caaagcccta tgtacagaac tcaatgcaca aggcagtatt
   109321 aacgtcgtca attattccca acatgatgaa caggatggag ttaagtaatg aatccactga
   109381 aagccggtga tatcgcaccg aaatttagct tgccggatca agacggagaa caagttaatt
   109441 tgaccgactt ccagggacag cgtgttctgg tttatttcta cccgaaagcc atgacccccg
   109501 gctgtaccgt acaggcctgc ggcttacgcg ataacatgga tgagttgaaa aaagcgggcg
   109561 ttgatgtgct gggtatcagc accgataaac ccgaaaaact ctcccgtttt gcggaaaaag
   109621 agctgcttaa ctttacgctc ctgtctgatg aggaccacca ggtgtgcgaa caattcggcg
   109681 tctggggtga aaagtccttc atgggcaaaa cctacgatgg tattcatcgc atcagcttcc
   109741 tgattgacgc tgatggcaaa atcgaacatg tctttgacga tttcaaaacc agcaatcacc
   109801 acgacgttgt gctgaactgg ctgaaagaac acgcctgatt actttgctcc attccgtgct
   109861 ggctgcgctt gcggccagca tacctcactt ctcgtgatca agatcacatt ctcgctttcc
   109921 cctgcgacac gggtgtcgaa tccatttttt gctgaacgtt aatgaccatc atttttgtac
   109981 cgttcagaat ccagttaata cataacttat tgaatagatt gagttaatca gaatggcatc
   110041 ctttatacaa tatgaaatgc aatgtttcat atcattttca aggagccgac atgaaccgct
   110101 ttgtggtggc cgaaccactg tggtgtacag gatgtaatac ctgtctcgct gcctgttcgg
   110161 acgtgcataa aacgcaaggt ttacagcaac acccgcgcct ggccctggcg aagacgtcaa
   110221 caatcactgc ccctgtcgtg tgtcatcact gtgaggaagc cccttgcctg caggtctgcc
   110281 cggtcaatgc catctctcag agggatgatg cgatccaact caacgaaagc ctctgtattg
   110341 gctgcaagct ttgcgccgtg gtctgcccat ttggcgcaat cagcgcttca ggaagccgtc
   110401 cggtgaatgc ccatgcgcaa tatgtttttc aggctgaagg ctcactcaaa gacggcgaag
   110461 aaaacgtgcc gccccaacat gcgttgctgc gctgggaacc gggtgtccag accgtcgcgg
   110521 tgaaatgcga cctgtgtgat ttcttgccag aaggtccggc ctgcgttcgc gcttgcccga
   110581 atcaggcgtt acggctgatc accgacgata gcctgcaacg tcagatgaaa gaaaaacagc
   110641 gccttgccgc aagctggttt gccaatggcg gggagtctcc cctttccctc actcaggagc
   110701 aacactaatg gatgccctgc aattattaac ctggtcgctg atcctctatc tgtttgccag
   110761 tctggcttcg ctgtttttac tcggtctgga cagactggct attaagcttt ccggcatcac
   110821 atcgctggtg ggcggcgtga ttggcatcat cagcggaatt acgcaattac atgctggtgt
   110881 aactttagtc gcccattttg ccaccccttt tgactttgcc gatttaaccc tgcgaatgga
   110941 tagcctctcg gcatttatgg tgctagttat ctccttgctg gtggtggttt gttcgctcta
   111001 ttcattgact tatatgcgcg aatacgaggg caaaggcgcg gcggcgatgg gcttctttat
   111061 gaatcttttc atcgcatcga tggttgccct gctggtgatg gacaacgctt tttggttcat
   111121 cgtgctgttt gaaatgatgt cgctgtcttc ctggtttctg gtcattgcca ggcaggataa
   111181 aacgtcgatc aacgctggca tgctctactt ttttatcgcc cacgccggat cggtgctgat
   111241 tatgatcgcc ttcttgctga tggggcgcga aagcggcagc ctcgattttg ccagtttcgc
   111301 acgctttcac tttctccggg gctggcgtcg gcggtgtttc tgctggcctt tttcggtttt
   111361 ggcgcgaaag ccgggatgat gccgttgcac agctggttgc cgcgcgctca ccctgccgca
   111421 ccatcgcacg cttcggcgtt gatgtctggc gtaatggtca aaatcggtat tttcggcatc
   111481 ctgaaagtag cgatggatct gctggcgcaa acgggtttgc ctctgtggtg gggcattctg
   111541 gtgatggcga tcggcgcaat ctccgcgctc ctgggcgtgc tatatgcgct ggcggaacag
   111601 gatatcaaac ggctgctggc ctggagtacc gtcgaaaacg tcggcattat tttgctggca
   111661 gtcggtgtgg cgatggacgg tctgtcactg cacgacccgc tgctcaccgt ggttggactg
   111721 ctcggcgcac tgtttcatct gctcaaccat gcgctgttca aagggctgct atttctcggc
   111781 gcggacgcga ttatttcgcg tttgcatacc cacgacatgg aaaaaatggg ggcactggcg
   111841 aaacggatgc cgtggacagc cgcagcatgc ctgattggtt gcctcgcgat atcagccatt
   111901 cctccgctga atggttttat cagcgaatgg tacacctggc agtcgctgtt ctcactaagt
   111961 cgtgtggaag ccgtagcgct acaacttgcg ggtcctattg ctatggtaat gctggcagtc
   112021 actggtgggc tggcagtaat gtgcttcgta aaaatgtacg gtattacttt ctgtggtgcg
   112081 ccgcgcagta cacacgctga agaggcacag gaagtgccaa atacgatgat cgtcgccatg
   112141 ctactgctcg cggcactctg cgtattaatt gcgcttagtg ccagttggct ggcaccgaag
   112201 ataatgcata ttgcccatgc gtttaccaat acccctcccg tcactgtcgc cagcggaata
   112261 gcacttgtac ccggcacgtt tcatacacgg gtcactcctt cattactgtt gctgttacta
   112321 ctggcgatgc ctttgctgcc aggcctttac tggctgtggt gtcgttcgcg ccgcgcagcg
   112381 tttcgtcgca caggagatgc ctaggcatgc ggctacggct gggaaaacgc gatggctccg
   112441 tcaggcaatg gcgtgatgca gccgctgcgt gtggtcttct ctgcgttatt tcgtctacga
   112501 caacagctcg accctacgct gaggctaaat aaaggtcttg cgcacgtcac cgccagggct
   112561 cagagcacag aacccttctg ggatgagcgg gtgatccgcc ccatcgtgag cgccacccaa
   112621 cggctggtca aagaaataca gcatctgcaa agcggcgact ttcgtctcta ttgcctgtat
   112681 gtggtcgccg cactggttgt gctgctaatc gctattgccg tctaaggaaa tcaccatgag
   112741 acaaactctt tgcgacggat atctggtcat ttttgcgtta gcacaggccg tgattctgct
   112801 gatgctaacc ccacttttta cgggtatttc ccggcagata cgcgcgcgta tgcactcccg
   112861 ccgcgggccg gggatctggc aggattatcg cgatatccac aaactgttta aacgccagga
   112921 agttgcgccg acatcttcag gtctgatgtt ccgcctgatg ccgtgggtat taatcagcag
   112981 catgctggtg ctggcgatgg ccttaccact atttattacc gtttcccctt ttgcgggcgg
   113041 cggcgatctg atcaccctta tctatcttct tgccctgttt cgttttttct ttgctctttc
   113101 cgggctggat accggaagtc cgtttgcggg agtcggtgcc agtcgcgagt tgacgctcgg
   113161 cattctggtc gaaccaatgc ttattctctc actgctggta ttggcgctga tagcaggttc
   113221 cacgcatatc gagatgatca gcaatacgct ggctatgggc tggaactcgc cgctaaccac
   113281 cgtactggcg ttactggcct gtggttttgc ctgcttcatt gagatgggaa aaattccctt
   113341 tgatgttgct gaagcagaac aggaattaca ggaaggcccg ctgaccgaat attccggtgc
   113401 cgggctggcg ctagcgaaat gggggctggg gctgaaacag gtcgtgatgg cagcactgtt
   113461 tgtggccctg tttctgccct ttgggcgcgc gcaagaactt tctctcgcct gcctgctgac
   113521 ttcacttgtc gttacgctgc tcaaggtttt gctgattttt gtactggcct caatcgcaga
   113581 aaacacgctg gcacgcgggc gttttttact cattcaccat gtgacctggc ttggcttcag
   113641 ccttgctgcg cttgcatggg tcttttggtt aaccggtctg taaggagcac tgacggaata
   113701 tggaaaatct tgctctgacg acgttaatgc tgccttttat cggcgcactg gtcgtttcgt
   113761 tttcgccaca acgtcgggcc gccgaatggg gggttttgtt cgccgcgctg accacgctgt
   113821 gcatgttgtc gctgatctcc gcgttttatc aggccgataa agttgccgtc acgttgacgt
   113881 tggtcaacgt gggggatgtg gcattgtttg gcctggtcat tgatcgcgtg agtacgctga
   113941 ttctgtttgt ggtggtgttt ctcggtttgc tggtcacgat ctactccacg ggttatctga
   114001 cggataaaaa tcgcgaacac ccgcataacg gcacgaatcg ttattacgca tttttgctgg
   114061 tgtttatcgg cgcgatggcg ggactggtag tctcctcgac gctgctcggt cagttgttgt
   114121 tttttgaaat tacgggcggc tgctcctggg cgttgatcag ttattaccag agcgataaag
   114181 cgcagcgttc agcactaaaa gcgttgctta tcacccatat cggttcgttg gggttgtatc
   114241 ttgccgccgc cacgctgttt ttgcagaccg gaacgtttgt gcttagcgcg atgagcgagt
   114301 tacatggcga cgcacgttat ctggtttatg gcggcatcct gtttgccgcg tgggggaaat
   114361 cggcccagct accgatgcaa gcgtggctac cggacgcaat ggaagcgcca acaccgatca
   114421 gcgcctatct ccacgccgca tcgatggtga aagtgggcgt ttacattttt gcccgcgcca
   114481 ttatcgacgg cggcaatatc ccgcatgtga ttggcggcgt tggcatggtc atgacactgg
   114541 tcaccattct ttatggcttt ctgatgtatt tgccacagca ggatatgaag cggttgctgg
   114601 cctggtcgac catcactcaa cttggctgga tgttcttcgg cttgtcgctc tccatcttcg
   114661 gctcgcggct ggcgctggag ggtagcatcg cctacatcgt caaccacgcg ttcgctaaaa
   114721 gcctgttttt ccttgtagca ggtgcgctga gttacagctg cggcacgcgc ttgttgccgc
   114781 gtctgcgtgg cgtattgcac accctgccgt tgccaggcgt gggtttctgc gtggcagcgc
   114841 tggcaattac cggtgtgccg ccgttcaacg gcttcttcag taaattcccg ctgtttgccg
   114901 ccggttttgc gttgtcagtg gagtactgga tcctgctgcc cgccatgatt ctgctgatga
   114961 ttgaatcggt cgccagtttc gcctggttta ttcgctggtt tggtcgcgtc gtgccgggca
   115021 aaccgagcga ggccgtcgcc gatgccgcac cgctgccagg gtcaatgcgc ctggtgttga
   115081 ttgtactgat tgtgatgtcg ctgatttcca gcgtaatcgc cgcgacctgg ttgcagtaag
   115141 gagatgatga atgaccggtt ctatgatcgt aaataatctg gcgggactga tgatgctgac
   115201 atcgctgttt gtgattagcg tcaaaagcta tcgcctgtca tgcggatttt acgcctgcca
   115261 gtcgctggtg ctggtgtcta ttttcgccac tctctcgtgc ctgttcgccg cagagcaact
   115321 gctgatctgg tccgccagcg cctttatcac caaagtgctg ctggtaccgt taatcatgac
   115381 ttacgctgca cgaaatattc cccagaacat cccggaaaaa gcgttattcg gtccggcaat
   115441 gatggcactg ctcgcggcgt taattgtcct gctttgcgca tttgtcgttc agcccgtgaa
   115501 gctaccgatg gctaccgggc tgaaaccggc gctggcggta gcgttaggtc attttctgct
   115561 tggcctgctg tgcattgtca gccaacgcaa tatcctgcgg caaatttttg gttactgcct
   115621 gatggaaaac ggctcccatc tggtgctggc gcttcttgcc tggcgagcac cggaactggt
   115681 ggaaataggt atcgctaccg acgccatctt cgccgtcatt gtgatggtgt tactggcaag
   115741 aaaaatatgg cgtacccacg gcacgctgga cgtgaacaac ttgaccgcgc tgaagggata
   115801 atgagatgag ttattctgtg atgttcgctt tactcctgct cacgccgctg cttttttcgc
   115861 tgctctgttt tgcctgccgg aaacgggggc tctctgcgac ttgtacggtg actgtattac
   115921 atagcttagg gatcacactg ctgctgattc tggcactctg ggtggtccaa actgccgctg
   115981 atgcaggaga aatattcgct gcgggactgt ggcttcatat tgatggtctg ggcggtttgt
   116041 tcctcgccat tcttggtgtg attggctttc tcaccggtgt ttactcgatt ggctacatgc
   116101 gtcatgaagt ggcacacggc gagctttcac ccgttacact gtgcgattac tacggtttct
   116161 tccatctgtt tttgttcacc atgctgctgg ttgttaccag caataacctg attgtgatgt
   116221 gggcggcgat cgaagccacc accttaagct cggcgtttct ggtaggcatt tacggtcagc
   116281 gttcatcgct ggaatcagca tggaagtaca tcattatttg tattgttggt gtcgcttttg
   116341 gtctgttcgg taccgtgctg gtatacgcca acgccgccag cgttatgccg caggcagaaa
   116401 tggcgatatc ctggagcgag gttcttaagc aatcgtcctt gcttgaccca acattaatgc
   116461 tgttggcctt tgtgtttgtg ctaattggct ttggtaccaa aaccgggcta tttcccatgc
   116521 acgcctggct gccggatgct cacagtgaag ctccgagtcc ggtcagcgcc ctgctctccg
   116581 ccgtattgct gaactgcgcg ctgttggtgc tgattcgcta ttacatcatt atttgccagg
   116641 ccatcggcag cgatttcccc aaccggttgt tgctcatctt cggcatgttg tcggttgccg
   116701 tggcggcatt tttcattctg gtacagcggg acattaagcg tctgctggcg tactccagcg
   116761 tggagaacat ggggctggtc gcggtggcgc taggcattgg cgggccgctg ggaatttttg
   116821 ccgcgctgct gcacacctta aaccacagtc tggcaaaaac gctgctgttc tgcggttccg
   116881 gcaatgtact gctcaagtac ggcacgcgcg atctcaacgt cgtctgtggg atgctcaaaa
   116941 tcatgccatt taccgccgtg ctgtttggcg gcggtgcgct ggcgctggcg ctggcaggga
   117001 tgccgccctt caacattttt cttagcgaat ttatgaccgt taccgcagga ctggcacgta
   117061 atcacctgct gattatcgtc ctgctgttat tgctgttaac gctggtgctg gcgggcctgg
   117121 tacggatggc tgcgcgggtg ttaatggcga aaccgccgca ggccgttaac cggggtgatc
   117181 tcggctggtt gaccacctcg ccaatggtga ttctgctggt catgatgctg gcgatgggaa
   117241 cgcatattcc acaacctgtc atcaggatcc tggcgggcgc ttccactata gtcctctcag
   117301 ggacgcacga cctgcctgca caacgtagca cctggcatga ttttttgcct tcaggcaccg
   117361 catctgtttc ggagaaacac agtgaacgtt aattcatcgt caaatcgtgg cgaagcgatt
   117421 ctcgccgccc tgaaaacgca gttccccggc gcggtgctgg atgaagagcg acaaacgcct
   117481 gaacaggtca ccattacggt gaaaatcaat ctgctgcctg acgttgtaca ttatctttat
   117541 tatcaacatg atggctggct tccagtcctg tttggcaacg acgagcggac acttaacggt
   117601 cattacgcgg tttattatgc cctttcaatg gaaggggccg aaaaatgctg gatcgtggtg
   117661 aaggcactgg tcgatgccga cagtcgggag tttccgtcag tcacaccgcg cgtccctgcc
   117721 gcggtctggg gcgagcgaga aattcgcgat atgtacgggc tgattccggt tggcctgccg
   117781 gatcagcgtc gcctggtgtt gcccgatgac tggccggaag atatgcatcc gctgcgcaaa
   117841 gatgcgatgg attatcgact gcgccctgaa ccgacgacag attccgaaac gtatgcgttt
   117901 atcaatgagg gcaacagcga tgcgcgggtg atccctgtcg gcccgctgca tatcacctcc
   117961 tatgaaccgg gtcacttccg cttgtttgtg gatggcgagc aaattgtcga tgctgattac
   118021 cgcctgtttt atgtccatcg cggcatggag aaactggcag aaacgcggat gggctacaac
   118081 gaagtgacct tcttatcgga ccgcgtgtgt gggatttgcg gttttgccca cagtgtggcc
   118141 tataccaact cggttgaaaa tgcactgggg attgaggtgc cgcaacgagc acatactatt
   118201 cgctcgattc tgctggaagt cgagcggcta cacagtcatt tgctcaacct tggcctctcc
   118261 tgccattttg ttggttttga taccggcttt atgcaatttt tccgcgtgcg ggaaaagtcg
   118321 atgacgatgg cggaattgct gaccgggtcg agtaaaacct acggtctgaa tctgattggt
   118381 ggtgttcgcc gcgatattct caaagagcaa cgtctgcaaa cgctgaaact ggtgcgcgag
   118441 atgcgcgccg acgtgtcgga gctggtagag atgctgcttg ctacgccgaa tatggaacaa
   118501 cgcactcagg gcattggcat tctcgaccga caaatcgccc gtgattatag ccctgtaggg
   118561 ccgctgatcc gcgcagtggt tttgcccgtg atttgcgctt tgatcacccc tacgccgact
   118621 acggcaatat tccaaaaaca ctgtttacct ttaccggcgg cgatgttttc tcccgcgtga
   118681 tggtccgtgt caaagagacg tttgattcgc tggcaatgct ggaatttgcc ctcgacaaca
   118741 tgccggatac cccactgctg accgaaggct ttagctataa acctcacgca ttcgcgctgg
   118801 gctttgttga agcgccacgc ggtgaagacg tgcactggag catgctcggt gataaccaaa
   118861 aattgttccg ctggcgctgt cgtgccgcca cctacgccaa ctggccggtg ttgcgttaca
   118921 tgctgcgcgg caataccgtt tctgacgcac cgctgattat cggtagcctt gatccctgct
   118981 actcctgtac cgaccgtgtg acgctggtag atgtgcgcaa gcgccagtca aaaaccgtgc
   119041 tgtataaaga gatcgaacgc tacggcattg atcgtaaccg ttcgccgctg aagtaaggac
   119101 agaagatgct gaagttactg aaaactatta tgcgcgccgg aaccgcgacg gtgaaatatc
   119161 ccttcgcgcc actggaggtc agcccaggct ttcgcgggaa accggacctg atgcccagcc
   119221 aatgtattgc ctgcggtgcc tgcgcctgtg cttgtccggc aaatgcgctg actatccaga
   119281 ccgacgacca gcaaaattcg cgcacctggc agctctatct ggggcgttgt atttactgcg
   119341 gacgttgtga agaagtgtgc ccgaccagag ccatccagct taccaataac tttgaactga
   119401 ccgtcaccca taaagccgat ctctataccc acgcgacgtt ccatctacaa cgttgcagcc
   119461 gttgcgaacg cccgtttgcc ccgcaaaaaa ccgtcgcact ggctgctgaa ttgttggcac
   119521 agcaacaaaa tgctccacaa aaccgcgaaa tgttatgggc gcaagcgagc gtctgcccgg
   119581 agtgcaaaca acgcgcgacg ctgatcaacg acgatacaga tgtaccgctg gtggctaagg
   119641 agcagctatg agtccagtgc ttacgcaata tgtcagccag cccatcacgc tggacgagca
   119701 aacgcaaaag atgaagcggc atttgctaca ggatatccgt cgctcggctt acgtttatcg
   119761 catcgattgc ggcggctgca acgcctgtga aatcgaaatt tttgctgcca tcacccccgt
   119821 attcgacgca gaacgttttg gcattaaggt tgtttcatca ccgcgtcacg ccgatatttt
   119881 gttatttact ggcacagtca cccgggcgat gcgtatgcct gcacttcggg cgtatgagtc
   119941 tgcccccgat cataaaatct gtgtttccta cggcgcgtgc ggtgtcggcg gcggtatttt
   120001 ccacgatctc tacagcgtct ggggcggcag cgacaccatt gtccccattg atgtttggat
   120061 ccccggctgc ccgccaacac cggccgccac cattcacggt ttcgccgtgg cgctcggttt
   120121 gctgcaacag aagattcacg cggtggatta tcgcgatccc accggggtga ctatgcaacc
   120181 gttgtggccg cagatcccgc catcacagcg tatcgccatt gagcgagaag cgcggcggct
   120241 ggcgggctat cgtcaggggc gagaaatttg cgatcggctc ctgcgccatt taagcgacga
   120301 tcctacagga aatcgggtta acacctggtt gcgcgatgcc gacgatctac gtctcaatag
   120361 tatcgttcag caactctttc gcgtactccg ggggttacat gactgaagag tgcggggaaa
   120421 ttgttttctg gacgctgcga aaaaagtttg tcgccagtag cgacgagatg ccggaacaca
   120481 gctctcaggt aatgtattac tcgctggcta tcggtcatca cgttggcgtg attgattgtc
   120541 tgaatgtcgc cttccgctgc ccactgacgg aatacgaaga ttggcttgca ctggtcgaag
   120601 aggagcaagc ccgacgtaag atgctggggg tgatgacttt tggtgagatt gttattgacg
   120661 ccagccacac cgccctgttg acccgggcat tcgcgccact ggcagatgac gcgacgtctg
   120721 tgtggcaagc gcgtagcatt caattcattc atctgttgga tgaaattgtg ctggaaccgg
   120781 ctatctatct gatggccaga aaaattgcgt gagaaggatt tctcattaat aaggactgtt
   120841 gatggctatg tcagacgaag cgatgtttgc cccgccgcaa ggaataacaa ttgaagcggt
   120901 aaacggaatg ctcgcggagc ggttagcgca gaaacacggc aaggcgtctt tattacgcgc
   120961 cttcatcccg ctgccgccgc cgttcagccc ggtacaactt attgaactgc atgttctcaa
   121021 aagcaacttc tattaccgct accatgatga tggcagcgat gtgacggcaa caacagagta
   121081 tcagggcgag atggtcgatt attcgcgtta cgccgtcctt ctcggcagta gtggaatggc
   121141 ggagctacgc tttattcgta cccacggcag ccgttttact ccccaggatt gcacactgtt
   121201 taactggcag gcacgcatta tcaccccggt tctgcaatca tggctcaatg atgaagcaca
   121261 gcaggtggcg ctgcgtttgc tggagaaaga tcgcgatcat catcgggtac tggtggatat
   121321 cactaatgca gtgctgtcac atcttgatct cgacgatctg atcgctgacg tcgctcgtga
   121381 gatccatcat tttttcggtc tggcttcagt cagtatggta ctgggcgatc atcgaaagaa
   121441 cgagaagttc agcctgtggt gcagcgatct ttctgcctca cattgtgcgt gtctgccaca
   121501 caatatgcct ggcgacagtg tattgctgac acaaacgcta caaacccgac aaccgacctt
   121561 gacgcaccgt gcagatgatc tgtttctctg gcaacgcgac ccgttattac tcttacttgc
   121621 atctaacggc tgcgaatctg cgctccttat accgcttacc tttggcaacc atacaccggg
   121681 tgcattgttg ctggcgcata cctcttccac tctctttagt gaggaaaact gccagctact
   121741 acaacacata gccgatcgca tcgctattgc cgttggcaat gccgatgcct gccgtagaat
   121801 gaccgatttg caggaaagtt tgcagcaaga aaaccaccag cttagcgagc agctcctttc
   121861 gaatctgggc gtcggtgaca ttatctatca aagccaggca atggaagacc tgctccagca
   121921 ggtagatatt gtggcgaaga gcgacagtac ggtgttgatt tgcggtgaaa ccggaaccgg
   121981 caaagaggtg atcgccagag cgatccatca acttagcccg cgacacgaca agccgctggt
   122041 caaaatcaac tgcgctgcca tccccgccag tcttctggaa agtgagttat tcggtcatga
   122101 caaaggggcg tttactggtg cgattaatac ccatcgtggt cgttttgaaa ttgccgatgg
   122161 cggcacgttg tttctcgatg aaattggcga tctgccgtta gaacttcagc ctaaactgct
   122221 gcgcgtattg caggaacggg agattgagcg tctcggcggg agtagaacga tcccggtaaa
   122281 tgtcagagtc attgccgcca ccaaccgtga tttgtggcaa atggttgaag atcgccagtt
   122341 tcgcagcgat ctcttttatc gcctgaatgt cttcccactg gaattgcctc cgctgcgcga
   122401 ccgtccggaa gatatccctc ttttagcaaa gcatttcacg caaaaaatgg cgcgccatat
   122461 gaatcgttca attgacgcca tcccgaccga ggcgctacgc cagttgatgt cgtgggactg
   122521 gccgggcaac gtgcgcgagc tggaaaacgt gattgagcga gcggtactgc tgactcgcgg
   122581 taacagtctg aatttacatc ttaatgtccg acaaagccgt ttactgccga cgctaaatga
   122641 agattcagcg cttcgcagtt caatggcgca gttgctgcac ccgacgacgc cagagaatga
   122701 cgaagaagaa cgtcagcgca ttgttcaggt attgcgagaa accaatggca ttgttgccgg
   122761 gccccgtggc gcggcgacac gattagggat gaagcgcacc acgctgctgt cacgaatgca
   122821 gcgtctggga atctcggttc gcgaggtgtt gtaatctgct tttgcaggag tatgcatgag
   122881 aaacaaactc tctttcgact tgcagttgag cgccagaaaa gcggcaatcg ctgaacggat
   122941 tgccgcccat aaaattgccc gcagtaaagt gtcggtcttt ttaatggcga tgtccgctgg
   123001 cgtgtttatg gcgatcggat ttacttttta cctttccgtt atcgccgatg ccccgtcttc
   123061 ataggcatta acccatctgg tgggcggcct ttgctttaca cttggcttta ttttgctggc
   123121 ggtttgcggc accagcctgt tcacctcgtc ggtaatgacg gtaatggcaa aaagtcgggg
   123181 cgttattagt tggcgaactt ggctgattaa cgcacttctg gtggcctgcg gtaatctggc
   123241 aggtattgcc tgtttcagtt tgttaatctg gttttccggg ctggtgatga gtgaaaacgc
   123301 gatgtgggga gtcgcgattt tacactgcgc cgagggcaaa atgcatcata catttactga
   123361 atctgtcagc ctcggcatta tgtgcaatct gatggtttgc ctggcgctgt ggatgagtta
   123421 ttgcggacgt tcgttatgcg acaaaatcgt cgccatgatt ttgtccatca ccctgtttgt
   123481 cgccagtggc tttgagcact gtatcgccaa tttgtttgtg attccgttcg ccattgccat
   123541 tcgccatttc gcccctacct ccttctggca actggcgcac agtagcgcag acaattttcc
   123601 ggcactgacg gtcagccatt ttattaccgc caatctgctc ccggtgatgc tgggtaatat
   123661 tatcggcggt gcagtgctgg tgagtatttg ttatcgggct atttatttac gtcaggaatc
   123721 ctgaaaaatc agcccggcga aacagttcgt cgggctgaag attattcttg cgcgatttgc
   123781 ccatcgggcc aggcgtggat cacggctttg atcagcgtcg ccaatggaat ggcgaagaat
   123841 acgccccaga atccccacaa accaccgaag atcaccaccg ataaaataat caccagcgga
   123901 tgcaggttaa ccgcttcgga gaacaacacc ggtactagca ggttgccgtc cagcgcctga
   123961 ataatcagat acaccgcgaa gcagctccag aattcagtac ctgcgccaaa ctggaatagc
   124021 gccacgccaa ccaccggaat ggtcaccaca aatgcgccaa tgtacggaat aagaaccgag
   124081 aagccgacca gcactgccag cagcagcgaa tagttcagcc cgaagagcaa gaaccccagc
   124141 caggtggcga tccccaccac gatcatctcc agcactttgc cgcggatata gttggtgatt
   124201 tgttgattca tctccttcca cacctgtcct gccagcccac ggttacgcgg cagcacccgg
   124261 cgaacggcgt tcagcatctg ctctttgtct ttcagcagga agaagaccat caatggcacc
   124321 agcaccagat agacggctat ggtcagcaaa ccgaccagcg aggcgaggga aattttcacc
   124381 accgaatcgc ccatggtcag catccgactg cgcatatttt cggccattgc atcaataatg
   124441 cccgcgtcca ttaacaccgg atagcggcgc ggcaacgtgg cggcaaagtc agaaagctta
   124501 ttgagcatcc ccggcatatc gcggattaag tagatgccct gttgccaggc gataggcagt
   124561 accacgaacg ccatcagtag caatataccg acaaaaacca ccaatacaat cgacgtcgcc
   124621 cagcggcggg agcagccaat agattgcagg cgcacggttg gccattccag caaataagcc
   124681 agcacaatag ccaccagcaa cggagcaagc aggccactaa agaaaaagat aatgccaaat
   124741 ccggcaacta aaataaccag caaggcaatc gcttccgggt cgctaaaacg gcggcgatac
   124801 cattgcatca acatttcgag cataaagacc ttccctgaac ctcaagagcg ggattgcgat
   124861 ccgcaattgt atcgaaatgt cacaaaaaag acttcgcttt ttatgacgga ttcaggaaac
   124921 tgaaaagtca tttgagtggg ctaatcttcg ccgttacact caaaggcggc gaggtgggaa
   124981 cgatatttca cagtatcggt caaatgacta tcttcagaaa tacaggatag aggttatgtt
   125041 caggcagttg aaaaaaaacc tggttgcaac cctcattgct gctatgacca ttggtcaggt
   125101 agccccggcg tttgccgaca gcgcagacac cttgccggat atgggaacct ccgcaggaag
   125161 cacgctttcc attggtcagg aaatgcagat gggcgactat tatgtccgcc agctacgcgg
   125221 cagcgcgccg ttaattaatg acccgctgtt aacgcaatat attaattcgc tggggatgcg
   125281 tctggtttcg catgccaatt cggttaagac accgtttcat ttttttctga tcaacaacga
   125341 cgaaattaac gcctttgctt tctttggcgg caacgtggtg ctgcactctg ccctgttccg
   125401 ttattccgat aacgaaagtc aactggcttc agttatggcg cacgaaatct cccacgtcac
   125461 ccaacgtcac ctggcgcgag cgatggaaga tcaacaacgc agcgcgccgc tgacctgggt
   125521 cggcgcgtta ggttctattt tactggcgat ggccagtccg caggcgggga tggcggcgct
   125581 gaccggtaca ctggcgggaa cgcgccaggg gatgatcagt ttcacccagc aaaatgaaca
   125641 ggaagcggac cgcattggta ttcaggtgct gcaacgctcg ggattcgatc cacaggcgat
   125701 gccgaccttc ctggaaaaat tgctcgatca ggcgcgttac tcctcgcgcc cgccggaaat
   125761 tttattgact cacccgttgc cggaaagtcg tctggcagat gcccgcaacc gtgctaatca
   125821 gatgcgcccg atggtggtgc agtcgtcgga agatttctat ctggcgaaag cgcgcacact
   125881 ggggatgtat aattccggac gtaaccagct caccagtgat ttgctggatg aatgggcgaa
   125941 aggaaacgtt cgtcagcaac gagcggcgca atatggtcgt gctttacagg cgatggaagc
   126001 caataaatac gacgaggcgc gtaaaacgct gcaaccgtta ctggcggcag aacctggcaa
   126061 cgcatggtat ctcgatctgg ctaccgatat cgatcttggg caaaacaaag ccaatgaggc
   126121 aatcaatcgc ctgaaaaatg cccgcgattt acgcaccaat ccggtgttgc agctcaacct
   126181 ggcgaacgct tatctgcaag gcggtcaacc acaagaagcg gccaatattc tgaatcgcta
   126241 cacctttaat aataaagatg acagcaacgg ctgggatttg ctggcacagg cggaagccgc
   126301 gctaaataac cgcgatcagg agctggctgc gcgagcagaa ggttatgcgc tcgccggacg
   126361 actcgatcag gccatttcgc tgttgagtag cgccagttcg caggtgaaat taggcagcct
   126421 gcaacaagcg cgttacgatg cgcgcatcga ccagttgcgc cagctgcagg aacgctttaa
   126481 gccttatacc aagatgtaat tcgcattaaa ggaagaacaa tgaccaaaca ggtaaaaatc
   126541 taccataacc cacgctgttc aaagagccgg gaaacgctga acctgctgaa agaaaacggc
   126601 gtggagccag aagtggtgct ctaccttgag acacccgccg atgcggcaac gctgcgcgat
   126661 ttgctgaaaa tgctggggat gaatagcgcc cgtgaattga tgcgccagaa agaggatctt
   126721 tataaagaac taaacctggc tgacagttcg cttagcgaag aggcgctgat tcaggcaatg
   126781 gtggataatc cgaagctgat ggagcgcccg attatggtcg cgaatggcaa agcgcggatt
   126841 ggtcgtccac cggagcaggt actggaaatc gtgggttaag atgtgctcca tcgctagttg
   126901 aagcacattg ccggatgcga cgcttgccac gtcttatccg gcctacgaat actatggatt
   126961 aacttcgcgt aggtcaggcg ttcgcgccgc atccgacaat aaacacctta tctacaactt
   127021 cagaatttct ttcacaaacg gaatggtcag cttacgttgc gcggtaatcg acgcacgatc
   127081 caactgatcc aacgtcataa atagcgtgcg catttctctg tcgagccgct tcagcaagaa
   127141 acgccccaca tcttccggca gttcaaaacc acgcaaacgc gcgcgtaact gtagcgcctg
   127201 caacttatct tcatcagaaa gtggctgcaa tttgtagatc tgcccccagt cgagacgcga
   127261 cgcgagatcc ggtaatccca gattcaactg ccgcggtgga cgatcgccgg tgatcaacaa
   127321 ccgtgttttg cccgattcca gaattcgatt gtagagatcg aaaatcgcca tctcccacaa
   127381 ctcatcgcct gcaatacact caatgttgtc gatacagacc agcgacaaat gctccatacc
   127441 gtcgagcact tccggaacaa accaggtgcg tttatccagc gggacatagc ccaccgcatc
   127501 gccacgctgc gacaattccg cgcaagccgc gtgcagcaga tggctgcgcc ccgcgccttc
   127561 gcgtgcccag agatagatgt aaccgctatg ttcctgacgc agcacgtttt gcagcgcggc
   127621 cagtaaagag gagttatccc ccggccagaa acttgcaaag gtttcgtcgt caggaagata
   127681 aagtggcaaa gagagctgtg ccggtgtgtt cagagatacc tcaaccagga tttcacaaaa
   127741 tcgcgagaag tttaccacag aatcccatga tgtttgaacc gggcagcaac actgcccggt
   127801 cggtacatta tttgtctgtt atatccgcgt cttctgcgtc cagcaccact tcttcagggc
   127861 gcagcacact gatcagtttg aaaatcaaac ttaagccgat accgacgatg gtcgccagcg
   127921 ccataccttt taactccgcc gcgccgatgt ttaccttcgc gccactgacg ccgatgatca
   127981 aaatcacgga agtcaggatc aggttctgtg ctttgttgta atccactttc gattcgatca
   128041 aaacacgaat accggaagca ccaatgacac cataaagcag cagcgaaacg ccgcccatca
   128101 ccggcaatgg gatcatctgg atagcggcag ccagtttacc gacgcaggaa agcaggatag
   128161 cgaaaatcgc cgccccgccg ataacccagg tactgtaaac acgggtgatc gccatcacgc
   128221 caatgttttc tccgtaagtg gtatttggcg tagagccaaa gaagccggag atcacggtcg
   128281 acaggccgtt agcaaacatc gaacggtgca gacctggatc gcgcagcaga tcttttttga
   128341 cgatattagc cgttactacc aggtgcccta cgtgttcggc aataaccact aacgccgctg
   128401 gcagaatagt cagaatggca aaccactcga agcgcggcgt atagagggtt ggcagtgcaa
   128461 accagtgagc attaataatc ggcgtggtat cgacaattcc cattgcgaaa gagagcgcgt
   128521 accccaccag cacgccaatt aaaatcggga taattgccag gaaaccacga aacagcacgg
   128581 aacctaaaac cgtgaccgcc agggtggtaa tagagatgat gatggttttg gagtttggcg
   128641 tttgcccttc agccgggagt aaacccgcca tgccggcagc tacgcccgcc agctccagac
   128701 cgatgacggc aacgattgcg cccattgccg caggtggaaa cagcacgtcc aaccagccgg
   128761 tccccgcttt cttcacgata aaagaaacca ggcagaacag cacgccgcac ataataaagc
   128821 cgcccagcgc gacttcatac cctaacggca acagtaacaa taccggtgaa ataaaggcaa
   128881 agctggaacc aagataagcc ggaattttcc ctttacagat gaagagatac agcagcgttc
   128941 caataccgtt aaataacagt acagtcgccg ggttaatatg aaataagacg ggcaccagga
   129001 cggttgcacc aaacatggcg aacaaatgtt gcaaactaag cgggattgtc tgtaaaagtg
   129061 gcggtctttc actcaccccg atagcacggc gcgtcatagt attatcctct gtattatgtg
   129121 ttataggcgc tttactcaaa aaaaagccga ctcttaaagt cggctttaat tatttttatt
   129181 ctttatttcg taccaaagat tttgtcaccg gcatcgccga ggcccggaat aatgtatccg
   129241 tgctcgttca gtccctggtc aatcgatgca gtatacagtt cgacgtccgg gtgcgctttt
   129301 tccagcgcag cgataccttc tggcgcagcg accagcacca gaactttgat gctgctgcag
   129361 cccgcttttt tcagcaggtc gatggtcgcg ataacggaac caccggttgc cagcattggg
   129421 tcaacgatca gcgccatacg ctcatcgatg ttagaaacca gtttctggaa gtacggtacc
   129481 ggctccagcg tttcttcatt acggtacata ccgacaacgc tgatgcgcgc gctcggaacg
   129541 ttttccagca caccgtccat cataccaaga cccgcacgca gaattggcac aacggtaatt
   129601 ttcttacctt tgatctggtc gatttctacc gggccgttcc agccttcgat agttactttt
   129661 tccgtttcga ggtcggcggt cgcttcgtaa gtcagcaggc tacccacttc ggaagcgagt
   129721 tcgcgaaagc gcttggtgct gatatcttgc tcacgcatca gtcccagctt gtgtttgacg
   129781 agtgggtgtt tgacttccac gatcttcata ctctttctcc tttgaggggc agccacaaaa
   129841 aaaatcgacg gattatacct cctttcttca aggcggcaat attattttcg ttgactttag
   129901 tcaaaatgat aacagtttga gataaagtta ttttatattc aaatggttat gaaagaagat
   129961 tattccatcc gaaaactaac ctttgccctg gcacaagtct tctttcgccg cgcgcccggg
   130021 gaaaagacgt gcaaaaaggt tgtgtaaaac agtctcgcaa acgtttgctt tccctgttag
   130081 aattgcgccg aattttattt ttctaccgca agtaacgcgt ggggacccaa gcagtgaccg
   130141 ataaaacctc tcttagctac aaagatgccg gtgttgatat tgacgcgggt aatgctctgg
   130201 ttggaagaat caaaggcgta gtgaagaaaa cgcgtcgtcc ggaagtgatg ggcggtctgg
   130261 gcggcttcgg tgcgctgtgt gcattgccgc aaaaatatcg tgaacccgtg ctggtttccg
   130321 gcactgacgg cgtaggtacc aagctgcgtc tggcaatgga cttaaaacgt cacgacacca
   130381 ttggtattga tctggtcgcc atgtgcgtta atgacctggt ggtgcaaggt gcggaaccgc
   130441 tgtttttcct cgactattac gcaaccggaa aactggatgt tgataccgct tcagcggtga
   130501 tcagcggcat cgcggaaggt tgtctgcaat cgggctgttc tctggtgggt ggcgaaacgg
   130561 cagaaatgcc ggggatgtat cacggtgaag attacgatgt cgcgggtttc tgcgtgggcg
   130621 tggtagaaaa atcagaaatc atcgacggct ctaaagtcag cgacggcgat gtgctgattg
   130681 cactcggttc cagcggtccg cactcgaacg gctattcgct ggtgcgcaaa attcttgaag
   130741 tcagcggttg tgatccgcaa accaccgaac ttgatggtaa gccattagcc gatcatctgc
   130801 tggcaccgac ccgcatttac gtgaagtcag tgctggagtt gattgaaaag gtcgatgtgc
   130861 atgccattgc gcacctgacc ggcggcggct tctgggaaaa cattccgcgc gtattgccag
   130921 ataataccca ggcagtgatt gatgaatctt cctggcagtg gccggaagtg ttcaactggc
   130981 tgcaaacggc aggtaacgtt gagcgccatg aaatgtatcg caccttcaac tgcggcgtcg
   131041 ggatgattat cgccctgcct gctccggaag tggacaaagc cctcgccctg ctaaatgcca
   131101 acggtgaaaa cgcgtggaaa atcggtatca tcaaagcctc tgattccgaa caacgcgtgg
   131161 ttatcgaata atgaatattg tggtgcttat ttccggcaac ggaagtaatt tacaggcaat
   131221 tattgacgcc tgtaaaacca acaaaattaa aggcaccgta cgggcagttt tcagcaataa
   131281 ggccgacgcg ttcggccttg aacgcgcccg ccaggcgggt attgcaacgc atacgctcat
   131341 cgccagcgcg tttgacagtc gtgaagccta tgaccgggag ttgattcatg aaatcgacat
   131401 gtacgcaccc gatgtggtcg tgctggctgg ttttatgcgc attctcagcc cggcgtttgt
   131461 ctcccactat gccgggcgtt tgctgaacat tcacccttct ctgctgccga aatatcccgg
   131521 attacacacc catcgtcagg cgctggaaaa tggcgatgaa gagcacggta catcggtgca
   131581 tttcgtcacc gatgaactgg acggtggccc ggttatttta caggcgaaag tcccggtatt
   131641 tgctggtgat acggaagatg acatcaccgc ccgcgtgcaa acccaggaac acgctattta
   131701 tccactggtg attagctggt ttgccgatgg tcgtctgaaa atgcacgaaa acgccgcgtg
   131761 gctggatggt caacgtctgc cgccgcaggg ctacgctgcc gacgagtaat gcccccgtag
   131821 ttaaagcgcc agctctgccg ctggcgtttt tcaattcacc tgtaaatcgc aagctccagc
   131881 agtttttttc ccccttttct ggcatagttg gacatctgcc aatattgctc gccataatat
   131941 ccaggcagtg tcccgtgaat aaaacggagt aaaagtggta atgggtcagg aaaagctata
   132001 catcgaaaaa gagctcagtt ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga
   132061 caaatctaac ccgctgattg aaaggatgcg tttcctgggg atctattcca ataaccttga
   132121 tgagttctat aaagtccgct tcgctgaact gaagcgacgc atcattatta gcgaagaaca
   132181 aggctccaac gctcattccc gccatttact gggcaaaatt cagtcccggg tgctgaaagc
   132241 cgatcaggaa ttcgacggcc tctacaacga gctattgctg gagatggcgc gcaaccagat
   132301 cttcctgatt aatgaacgcc agctctccgt caatcaacaa aactggctgc gtcattattt
   132361 taagcagtat ctgcgtcagc acattacgcc gattttaatc aatcctgaca ctgacttagt
   132421 gcagttcctg aaagatgatt acacctatct ggcggtggaa attatccgta gcgatagcat
   132481 ccgttacgcg ctgctggaga tcccatcaga taaagtgccg cgctttgtga atttaccgcc
   132541 agaagcgccg cgtcgacgca agccgatgat tcttctggat aacattctgc gttactgcct
   132601 tgatgatatt ttcaaaggct tctttgatta tgacgcgctg aatgcctatt caatgaagat
   132661 gacccgcgat gccgaatacg atttagtgca tgagatggaa gccagcctga tggagttgat
   132721 gtcttccagt ctcaagcagc gtttaactgc tgagccggtg cgttttgttt atcagcgcga
   132781 tatgcccaat gcgctggttg aagtgttacg cgaaaaactg actatttccc gctacgactc
   132841 catcgtcccc ggcggtcgtt atcataattt taaagacttt attaatttcc ccaatgtcgg
   132901 caaagccaat ctggtgaaca aaccactgcc gcgtttacgc catatttggt ttgataaagc
   132961 ccagttccgc aatggttttg atgccattcg cgaacgcgat gtgttgctct attatcctta
   133021 tcacaccttt gagcatgtgc tggaactgct gcgtcaggct tcgttcgacc cgagcgtact
   133081 ggcgattaaa attaacattt accgcgtggc gaaagattca cgcatcatcg actcgatgat
   133141 ccacgccgca cataacggta agaaagtcac tgtggtggtt gagttacagg cgcgtttcga
   133201 cgaagaagcc aacattcact gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt
   133261 ctctgcgccg gggctgaaaa ttcacgccaa actgttcctg atttcacgta aagaaaacgg
   133321 tgaagtggtc cgttacgcac acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct
   133381 ttatactgac tattcgttgc tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt
   133441 atttaacttt attgaaaacc cataccgccc ggtgacattt gattatttaa tggtgtcacc
   133501 gcaaaactct cgccgtctgt tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca
   133561 agggctgccc agtggtatca ccctgaagct aaataacctt gtcgataaag gcctggttga
   133621 tcgtctgtat gcggcctcca gctccggcgt accggttaat ctgctggttc gcggaatgtg
   133681 ttcgctgatc cccaatctgg aaggcattag cgacaacatt cgtgccatca gtattgttga
   133741 ccgttacctt gaacatgacc gggtttatat ttttgaaaat ggcggcgata aaaaggtcta
   133801 cctttcttcc gccgactgga tgacgcgcaa tattgattat cgtattgaag tggcgacacc
   133861 gctgctcgat ccgcgcctga agcagcgggt gctggacatc atcgacatat tgttcagcga
   133921 tacggtcaaa gcacgttata tcgataaaga actcagtaat cgctacgttc cccgcggcaa
   133981 tcgccgcaaa gtacgggcgc agttggcgat ttacgactac atcaaatcac tcgaacaacc
   134041 tgaataaccc tatgccaata cacgataaat cccctcgtcc gcaggagttt gctgcggtcg
   134101 atcttggttc aaacagtttt cacatggtca tagcccgtgt ggtagatggt gccatgcaga
   134161 ttattggccg cctgaaacag cgggtgcatc tggcggacgg cctggggcca gataatatgt
   134221 tgagtgaaga ggcaatgacg cgcggtttga actgtctgtc gctgtttgcc gaacggctac
   134281 aagggttttc tcctgccagc gtctgtatag tcggcaccca tacgctgcgt caggcgctga
   134341 acgccactga ctttctgaaa cgcgcggaaa aggtcattcc ctacccgatt gaaattattt
   134401 ccggtaatga agaagcccgt ctgattttta tgggcgtgga acatacccaa ccggaaaaag
   134461 gtcgcaaact ggttattgat attggcggcg gatctacgga actggtgatt ggtgaaaatt
   134521 tcgaacctat tctcgttgaa agccgccgga tgggttgtgt cagctttgcc cagctttatt
   134581 ttcctggcgg ggtcatcaat aaagagaatt ttcagcgcgc tcgcatggcg gcagcacaaa
   134641 aactggaaac tttaacctgg caattccgta ttcagggctg gaacgttgca atgggcgctt
   134701 ccggtaccat aaaagccgcc catgaagtgt taatggaaat gggcgagaaa gacgggataa
   134761 ttaccccgga acgtctggaa aaactggtaa aagaaatttt acgtcaccgt aatttcgcat
   134821 cgctgagttt accgggtctt tccgaagagc ggaaaacagt cttcgttcct ggactggcga
   134881 ttttatgtgg tgtgtttgat gctttagcca tccgtgaact gcgcctttct gacggggcgt
   134941 tacgcgaagg cgtactgtat gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc
   135001 gcaccgccag cagcctcgcc aaccagtatc acatagacag cgaacaggcc cgacgagtgc
   135061 tggataccac tatgcaaatg tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc
   135121 cgcaactgga ggcgctactg cgatgggctg ccatgctgca tgaggtcggg ttgaatatca
   135181 accacagcgg tttgcatcgc cactccgctt atattctgca aaacagtgac ttgccgggtt
   135241 ttaatcagga acagcagctg atgatggcga cactggtgcg ctatcaccgt aaagcgatta
   135301 agctcgacga tctgccgcgc tttaccttgt ttaagaagaa acagttcctg ccactgatac
   135361 agctattgcg ccttggcgta ttactaaaca atcaacgtca ggcaaccacc acaccgccaa
   135421 cattgacact gattactgat gacagtcact ggacactgcg tttcccgcat gactggttta
   135481 gtcagaatgc gctggtactg cttgatctgg aaaaggagca agaatactgg gaaggcgtgg
   135541 ctggctggcg gttgaaaatt gaagaagaaa gtaccccaga aatcgccgct taatgctttg
   135601 cgggccgacg agaaatgtcg gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt
   135661 cattcagcgt atcaattaac ggttgcggct taccaataag ataaccctgc atataatcga
   135721 tccccaaaga gagcaccgcc tcgcggatct cttcgttttc aacgtactct gccactaccc
   135781 gcattttctt cattcgcgcc agatggcaaa tcgacgccac aatctgataa tccagactat
   135841 ttgacacaat attgcggata aagctgccgt caattttaag cagatcggca ttcacatttt
   135901 taagccgtgc atagctggcg tagccggtgc cgaaatcatc aatcgcaatc tggcagccta
   135961 attcctgaag atgctgcaag gtgatttgcg cctgcttaac attggtcaga gcattacttt
   136021 cggtgacttc aaaaataagt tgccacgctt caatctgata tttagccagc aactgactga
   136081 cttcaacagg aaaacgagcc tgacataccg aggttggaga cagattaata gcaaaacggt
   136141 gagcgggcat cttcgctctg ttttcagcca taaattgcag cgtatgctca atgacccaca
   136201 tgtcgatact cgacgataaa ccaaattcgt gcgcgaccgg caagaagcta ccggggccga
   136261 tcagttcatc attctcacct ttcatgcgca gaagaatttc atggtaaaca tcaccacgca
   136321 taccggtaat cggctgggcc atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc
   136381 gattcatcat cgcgacttta tctttcaatt cgcgttgcaa atacattgcc ccgcaacgct
   136441 gcatattttc cggggcgttg gtcacgatgg aaagttcggc gaccgtattt agctctccca
   136501 gcagcaggta gatatgattc actggcgagc gcacatagca gtaactgacg ccaatctgcg
   136561 gttgcatcgg catcccatcc caaaagaaac gaaattgctt gagatgacta tccagtgcgg
   136621 taatgcgctc ctggtgcgat tctgtattca gtcgcagcgc gagatcgtta cccgaaagct
   136681 gataaacatc ttcacccggt tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt
//
LOCUS       DAOFMX010000002       110995 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.002, whole genome shotgun sequence.
ACCESSION   DAOFMX010000002 DAOFMX010000000
VERSION     DAOFMX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 110995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 110995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..110995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.002"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            105..755
                     /locus_tag="QBA20_000132"
     CDS             105..755
                     /locus_tag="QBA20_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290822.1"
                     /translation="MANIIISKKSIIEAASIVSDELREKADLATQTYNEHYKNGTHTK
                     ADKANMQAATTKLAYFINNVVNAVEDEKLCSVFYYAIKASKQAPEVFFRDAMTNSYSL
                     EKLVYLVKSIKSGKCVYSIADMSGSRVFALIDMINDEIDTFTNGAVFDLMNEAKKACE
                     IKLDAGYTQANQLINLCERLGLVEKVKGAGSAKAGTQQYRFIKNDFYNYLADAFKA"
     gene            804..1007
                     /locus_tag="QBA20_000133"
     CDS             804..1007
                     /locus_tag="QBA20_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290823.1"
                     /translation="MVSYDKIRAEYRAKYRAYKLELIDDLIAQRDQLNFTFSDLLNSK
                     RDCKRKREYLRLSALIGKLQNSI"
     gene            1028..1258
                     /locus_tag="QBA20_000134"
     CDS             1028..1258
                     /locus_tag="QBA20_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290824.1"
                     /translation="MFVLIAGVNVHNEYYVNRIAGIAGYAGRAVELIDETTRKIDLLS
                     DQERKKADVNDADIFLMLKAFVEMGFEISLHK"
     gene            1640..1845
                     /locus_tag="QBA20_000135"
                     /pseudo
     CDS             1640..1845
                     /locus_tag="QBA20_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718033.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1875..2357)
                     /locus_tag="QBA20_000136"
     CDS             complement(1875..2357)
                     /locus_tag="QBA20_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290825.1"
                     /translation="MNFIATVNAPAHGNIAVTFSDIEKRVLGAWRDNETVELSAQEKC
                     IIARDIIGNRRYSRVFEKAYVVNSGFGTFVFPVRSGRFCQSKLIEFATQISVWIKTQS
                     SFKFSDDEAVSQGMRIANNAIKCKNITYTAGVDTWKLFCANFMLNVYASNRIHILDGV
                     "
     gene            complement(2562..2843)
                     /locus_tag="QBA20_000137"
     CDS             complement(2562..2843)
                     /locus_tag="QBA20_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDQ6290826.1"
                     /translation="MTDFTITPKAQNVFLESWLDLPETEQQEMDHVDYDEQVSTRFFH
                     FEGCVYDIADFMRDDRFPEWHASYPLNAFAMLMIRVDDSGDTIDIGLLH"
     gene            complement(3179..3553)
                     /locus_tag="QBA20_000138"
     CDS             complement(3179..3553)
                     /locus_tag="QBA20_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290827.1"
                     /translation="MGLDIYIETQPKNDLNNEASRKRVAYFRKFNALVGWMERNVGEV
                     ENCELLELTMNDICFLKAHLMHINESNCEEYLPTREGFFFGSQEYDEGYWHDVGELKE
                     LVEDLIKNHDFHNNRLTFCAWW"
     gene            complement(3671..4066)
                     /locus_tag="QBA20_000139"
     CDS             complement(3671..4066)
                     /locus_tag="QBA20_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290828.1"
                     /translation="MTVKREKLTVDVYYASETAEGKNVAKITVVTYNTETGAEVQAST
                     IVRKGDASGGEYATQYQSILDATDPLLLKIENYFRQVDEEVFETMMNMVNTVFASSLN
                     TSTTWIGQYGLRITSGIPADTLIPESVFA"
     gene            complement(4193..4504)
                     /locus_tag="QBA20_000140"
     CDS             complement(4193..4504)
                     /locus_tag="QBA20_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290829.1"
                     /translation="MKKSLVLGLDKDQKRKEKPALVAQLTLLDIVANGTSIRLFRETA
                     VSFDKNTFTRYVMNVRRQRGKGWMAFQRMWPEHQLELALMEVNRVAQQEIQRASVMAI
                     A"
     gene            complement(4658..4987)
                     /locus_tag="QBA20_000141"
     CDS             complement(4658..4987)
                     /locus_tag="QBA20_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290830.1"
                     /translation="MFANIDINQIKKLTQKEFDQFYELEGWSSTLINSRWVLELMTRD
                     DAPALMISDLGEDADFMDMSEFCVDTYNRSQKYYFTCDSENDVISKVYLHLVQHWDVQ
                     EFLEVFA"
     gene            complement(6562..7752)
                     /locus_tag="QBA20_000142"
     CDS             complement(6562..7752)
                     /locus_tag="QBA20_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290831.1"
                     /translation="MTDTAFSKSLQKEVDPEQYLALKNLDDSSVHAIARDDIICPICK
                     VGGGSFVRATRNGGYHKKAHFRFTGEDGQGHHPSCDFYGDRLTSEVKQHLVSFTKDRT
                     KYSQVIRKLVCAGIQEGIFTQEKMWQMREWFFNKRKDSTFEIWLESEHLDWLYYISGL
                     RDAYLAWTTPDILPFAPIQVTVPGFSWKRAIDKEVLRVHIKTLQQLREISVSHRDIAV
                     ILEHINNNRDRTILDPSHLEDEISKTYKLTSFVMGNYIEFQTKTVSDRAYGEAKFLAF
                     AALLLFVSDWDLNIAIGKFSKIARVKEVDDMLAGNFIGLNPYFRYDLANAVKRLQDNW
                     PIEYQELEHWNVEKSMRDAYEKWRATEPEFAPPLLPDLYIAKHEKEVAQDEQVRQWIK
                     NGDV"
     gene            complement(7923..8222)
                     /locus_tag="QBA20_000143"
     CDS             complement(7923..8222)
                     /locus_tag="QBA20_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290832.1"
                     /translation="MLNITQAYQVALISSSHTSEEDARRFMIASNELDWVSQIDGGCI
                     VHAGLQDDVWKDDLRRYGISEGAIANIQKVLDVGFDSVHFDCGAPIVEGLECWCW"
     gene            complement(8384..10417)
                     /gene="metG"
                     /locus_tag="QBA20_000144"
     CDS             complement(8384..10417)
                     /gene="metG"
                     /locus_tag="QBA20_000144"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDQ6290833.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDTT
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            10575..11675
                     /gene="apbC"
                     /locus_tag="QBA20_000145"
     CDS             10575..11675
                     /gene="apbC"
                     /locus_tag="QBA20_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDQ6290834.1"
                     /translation="MSESKSPDALRAMVAGTLANFQHPTLKHNLTTLKALHHVALMDD
                     TLHVELIMPFVWNKPFEDLKEQCSGDLLRITGAKAIDWKLSYNIATLKRVKNQPGING
                     VKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQRPT
                     SPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDYLVL
                     DMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMS
                     VHICSNCGHHEPIFGTGGAQKLAEKYNTQLLGQMPLHISLREDLDNGTPTVISRPDSE
                     FTAIYRDLADRVAAQMYWQGEVIPGEIAFRAV"
     gene            complement(11713..12102)
                     /locus_tag="QBA20_000146"
     CDS             complement(11713..12102)
                     /locus_tag="QBA20_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HDQ6290835.1"
                     /translation="MGFPSPAADYAESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCALDGEYRVKRYRKYPRQHLEDLRTGKKEALPKDDDGCT
                     GSNAVFGVITHIINDARSGEFDDCPVM"
     gene            complement(12899..13531)
                     /locus_tag="QBA20_000147"
     CDS             complement(12899..13531)
                     /locus_tag="QBA20_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290836.1"
                     /translation="MSTVAKDLTEDILNEIIAGANTSLEQLLALALRAERRDRNRFYE
                     RLPGKRPDRSDEEGCDIDYMEPSEIYQLGKDDGWNAYHDAVMKLDEKVKPLSRPVDHG
                     FRDNCECSSCQTTARICSELTDKSSLIYEVNVGGNTWVECTRAAYVRAKDKGELTRVV
                     AHHPNNELKDHQIRELVNELRDIAVQYHGTQQLREKIARAVNNSIRSESM"
     gene            complement(13528..13911)
                     /locus_tag="QBA20_000148"
     CDS             complement(13528..13911)
                     /locus_tag="QBA20_000148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290837.1"
                     /translation="MMHKSVAGEFQKEVDNTTDLLDDILSILALLEAGDWSEHCTKTE
                     LGGRLEREITRLISDAQNDLSPEHLPCDVVLVPGMRIHEGVRTKTLLTALQRRAELSS
                     RIKLMPSKIFDVVKFTPSQHTERDK"
     gene            complement(13908..14561)
                     /locus_tag="QBA20_000149"
     CDS             complement(13908..14561)
                     /locus_tag="QBA20_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDQ6290838.1"
                     /translation="MTTVNNKKCYPSEKYLNELITNIEFAARAPVEVVRAIAAELQKH
                     RKAYANTAASKDGWISCSERMPVIGELNWRTSFPLLVTCEIGVIPAYYGFVSVNGDRH
                     YGFMESLKYGDDNGNHPQTNEYGLISNVTHWMPLPERRRSRTVNNAELFQKISALATE
                     CHAIASELDVGDERTEMFEIYSVLRNLCRRGYATQVGRMTNPLLSSCDEDDSDEDDE"
     gene            complement(14561..14746)
                     /locus_tag="QBA20_000150"
     CDS             complement(14561..14746)
                     /locus_tag="QBA20_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290839.1"
                     /translation="MRTLEVRAEDVIPGDVLITSKGQQCAVKSFWMENDKVTLFGTDG
                     SETDYDYDELLVVERAA"
     gene            complement(14743..15273)
                     /locus_tag="QBA20_000151"
     CDS             complement(14743..15273)
                     /locus_tag="QBA20_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290840.1"
                     /translation="MKLSKFSELVNRILSNNHSHRRDMDVTIVVHSPGRIGSTPSVEV
                     QSIQAGFDWDAGQVMIFPSQPLTTLTPEQVADIADSVRKGQSWHAYQEYKKHKEQLEK
                     LSMELEAAKQREKDLFMENVRLKSGIAGLIHLGIRYADVEVMKIAGDAQLSTPCTDSI
                     INSIAAGIFTKEGAAR"
     gene            complement(15270..15593)
                     /locus_tag="QBA20_000152"
     CDS             complement(15270..15593)
                     /locus_tag="QBA20_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HDQ6290841.1"
                     /translation="MTTSHSAITQEKAFNILERLETLATEEEISPEKLVEFSRVILRR
                     KNDMERLTSGAPSLSVRRTLCCSFCNKSQYAVKKLIAGDAVFICDECVDVCNRIIRGE
                     KERSA"
     gene            complement(15590..15991)
                     /locus_tag="QBA20_000153"
     CDS             complement(15590..15991)
                     /locus_tag="QBA20_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDQ6290842.1"
                     /translation="MSKIDYQALREAAERAIPAMERLLMLPVDDDLICEQELKDSGVD
                     IDALNAFKFLAGPETVLALLDEINALEETRINDVCRIAELTKQLELAKSKLNEQREYY
                     EGVISDGSKRIAALLRKDNLASATNIEGERK"
     gene            complement(15988..16167)
                     /locus_tag="QBA20_000154"
                     /pseudo
     CDS             complement(15988..16167)
                     /locus_tag="QBA20_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16285..16488)
                     /locus_tag="QBA20_000155"
                     /pseudo
     CDS             complement(16285..16488)
                     /locus_tag="QBA20_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066192.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16488..17030)
                     /locus_tag="QBA20_000156"
     CDS             complement(16488..17030)
                     /locus_tag="QBA20_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290843.1"
                     /translation="MKVEKIDVLSFVLTDLERLDPVRVMIENYEPGKGRITIICYGKA
                     WTAAWFAMGGDDVQTFIKRVSNEYLIGYFDSQLRSTVDDDNDANLLFVKSEIIKLRRE
                     REIDAVLAREMWDEAENTDDVKESCCCFGVGNKLLNLFGDDPWYADWPTVPNPKYQYL
                     ERIVNAVREGLKELDKLEAS"
     gene            complement(17027..17668)
                     /locus_tag="QBA20_000157"
     CDS             complement(17027..17668)
                     /locus_tag="QBA20_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDQ6290844.1"
                     /translation="MSSISEAHMFAAGAHGGVGQKRKYTGEDYINHPVAVREIVALHD
                     GTVEMQIAALLHDVVEDTHVTIEMVRDHFGERVAEMVQALTNIARPEDGNRIQRFIIN
                     VRELEQNLDMQTRMIKLADLLDNTSSIVSRDPEFSAVYLAEKELMLDVLFHGKEIGAN
                     ADVVEYMERIGIEHPLLLSAMAKVTEGIALLKPVHIKRYEKHKSLIWSAWEAA"
     gene            complement(17788..18168)
                     /locus_tag="QBA20_000158"
     CDS             complement(17788..18168)
                     /locus_tag="QBA20_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290845.1"
                     /translation="MFGIDAQRIAAFAKSPLDNPLSRSEQMELARLFLHIQKQADIFN
                     NMPNQPILDGHIQMVINSHEKGWAAIVPCTITYKLAKEVQEFRKASVESESTKAAINT
                     LIRMGFTWTGGAYWQAPHPILSGN"
     gene            complement(18168..18872)
                     /locus_tag="QBA20_000159"
     CDS             complement(18168..18872)
                     /locus_tag="QBA20_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290846.1"
                     /translation="MNNLQLEHFNVTGHSDFPFKFTLKGYAEDAVGQIIIDKGIVKFE
                     GDFDESAKTFIDFVAKRWSEQWKNLEKRANEFDRFMDAMDTAKEALDAGTPLDLESLF
                     NGEVASAMFATMFAGEFVRSGAKNYLELDYNVPAIGDFVVTIQRKEGKTPGERVAELE
                     AVVDQRNGECDRLINELHALREERICAGSNTRNAADIYFQLVEECQIPPGGSLVDYVR
                     HLMAEVNSSHKDGEVR"
     gene            complement(18934..20619)
                     /locus_tag="QBA20_000160"
     CDS             complement(18934..20619)
                     /locus_tag="QBA20_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDQ6290847.1"
                     /translation="MNTALSIIDDASSNTAIDYRQEMNVIHEIVAECEKEIAFMYQVH
                     DFVYGDERHNMINRLLRLNHRPDEDRSRLNRGWLDKVDLEWVKQNIWAEYWRKVTDMT
                     NVLLIMPASRRDEWREQFIEGKQEVIKTDRTGYQMKVKEFVGVPEFKAETVIPTMLNL
                     LNDRHKYLSERVYGLFKALSPAHKTNKTNGFSERLIIADCISDFWRDSVSVNYRKEDY
                     IDDLRVLLHFFAHKEFITINRTAEVLSAAYRANDCQTGDWMNVDGNLMRVKMFKNGNV
                     HFEIHPDVAWKLNEVLAYSMPAAIPAPCRTAPKTRAPKQFGLIQKTISVPVRTALRDG
                     RLSKDKGVWYFSDSALQKSQVEELERTLSFIGGVQEKKHWQFPYEIGHTLNTIVATGL
                     IPDTKSHQFYPTPRLIAEYVARATELKSGEKLLEPQAGRGDLLAYINADLEDVTCIEI
                     APLFADILRGKGYTNTICCDFIKWSEDNVGYQFDKIVMNPPYSLGRHREHTLAALGHL
                     KVGGRLVAVLPGTAPILDWMTMDNYVYARGKSFTNEFEDTGITVSVYVFKRVK"
     gene            complement(20761..21327)
                     /locus_tag="QBA20_000161"
     CDS             complement(20761..21327)
                     /locus_tag="QBA20_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290848.1"
                     /translation="MCKKCKAIADEQNALFEEMDANELVKMLAILRGIEDVSIFERVV
                     TALNFESTFEEPTQIVALAHHFGVHYLAEKERADKLQATLDMVSETQGSHDGNKSETI
                     IASKDREIAGLKSSLTMLMSAINLMSSKAGYKMPSLNSDDPMAVRQLLGAMADQLDDT
                     KSRLEDMMRELTHRHDLNKQPHKTQYAL"
     gene            complement(21467..21625)
                     /locus_tag="QBA20_000162"
     CDS             complement(21467..21625)
                     /locus_tag="QBA20_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HDQ6290849.1"
                     /translation="MLERFRLVTVIALLVMAVLVDFTGKMMSVISDGVLIGLAIYFAY
                     PLVRKATC"
     gene            complement(21625..22050)
                     /locus_tag="QBA20_000163"
     CDS             complement(21625..22050)
                     /locus_tag="QBA20_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDQ6290850.1"
                     /translation="MLGLFKKKTRKAVIEVKKMENRDAVEATVWGAYMISYADGTCDA
                     KEISILEKTIAALPAFSPFAGEIAQMSANIRAQYEASPRRANAQALRELADVAGTNDA
                     VDVLCLCLDIADQDGIGEQEELALKKIAQALQLSLDAYL"
     gene            complement(22144..22341)
                     /locus_tag="QBA20_000164"
     CDS             complement(22144..22341)
                     /locus_tag="QBA20_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290851.1"
                     /translation="MNWFSNHFGKTWLAILAFIALMAAGWVSNIVKLVCSGDLQFQAG
                     MTLARVVGIFVFPVGSVLGYF"
     gene            complement(22351..22845)
                     /locus_tag="QBA20_000165"
     CDS             complement(22351..22845)
                     /locus_tag="QBA20_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290852.1"
                     /translation="MARQTYFTSATKRPRSLRQILAELFSGRVLSRLDELETAVRLLN
                     ERLDKQASVVANVGAIVTSGSSREAKSTRPLVKEKNNKDSSNGKFSKKEAETNGLRSH
                     YSFTGDGSRSSRPEPFDAGFIHHHTSVDDNYHHSSRASCHSGWDDGGCDTSSSSSYSG
                     SCCD"
     gene            complement(22991..23638)
                     /gene="rnhA"
                     /locus_tag="QBA20_000166"
     CDS             complement(22991..23638)
                     /gene="rnhA"
                     /locus_tag="QBA20_000166"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDQ6290853.1"
                     /translation="MCARARKKIGAALDSGVYMTTKTPARSQAKTRKKAKNKNSPRTN
                     PTTPVVEFNPQLKTVKIFSDGSCLKNPGGPGGYGIVLQYRGEEREFSDGFHSTTNNRM
                     EMMGALIGLERLKYPCNVILHSDSQYLKNGMTQWMKWWKRNGWVTSDKKPVKNVDLWK
                     RLDEAASRHNVRWKWVKGHAGHRENEICDRLAKIAAFSAADMPHKKDIGFVYNKQ"
     gene            complement(24169..24399)
                     /locus_tag="QBA20_000167"
     CDS             complement(24169..24399)
                     /locus_tag="QBA20_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290854.1"
                     /translation="MSYPTNVVALVESDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRENNQSQ"
     gene            complement(24586..25179)
                     /locus_tag="QBA20_000168"
     CDS             complement(24586..25179)
                     /locus_tag="QBA20_000168"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HDQ6290855.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGVERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATECANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRPSKKAA"
     gene            complement(25363..26172)
                     /locus_tag="QBA20_000169"
     CDS             complement(25363..26172)
                     /locus_tag="QBA20_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290856.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESVEAAENEPEQL
                     GFIEVEEYVEVNDELKVQQSNDSNTNKKARTARGPRFTLSDKDDSFFNKAGLETDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            complement(26333..26881)
                     /locus_tag="QBA20_000170"
     CDS             complement(26333..26881)
                     /locus_tag="QBA20_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDQ6290857.1"
                     /translation="MIATGVDIESTGLDFLSGHKIIEIAMVKYEIETQTMVDSFVMRF
                     NPRRSIDPKAQAVHGISLEDLAAEPLLADHASSVASYLSSSDIWIAHNGEAFDIPFIR
                     HELKSYGFSLPDIPLIDTLFSLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYDTNL
                     MMQCFFKARNKYGFFKLPSEIV"
     gene            complement(26878..27672)
                     /locus_tag="QBA20_000171"
     CDS             complement(26878..27672)
                     /locus_tag="QBA20_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290858.1"
                     /translation="MGQPVDITGKRFGKLVALKYSGISNKQGRLWDCICDCGNTCLVS
                     YGKLNHGATISCGCVYNAHRRSVKPHGMSKTPIYRIWLGMRERCEKPIHHAYKWYGGR
                     GIKVCERWQIFENFYADMGERPEGMSLDRKDVNGDYEPENCRWATFEEQANNTRSNLI
                     LEHKGEKLTLSQWAKRAGIQTSTLHYRIKKGWPLDRALNASVDTYANRDSKRLIECRG
                     RTQRITEWAREVGLTATIISQRILRGWDVEAAIFTPSKRPVKGDKK"
     gene            complement(27682..28101)
                     /locus_tag="QBA20_000172"
     CDS             complement(27682..28101)
                     /locus_tag="QBA20_000172"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290859.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEGSKKPLFPMHLMLAGGTVLYVSVCLDSNQINILN
                     TASGKWKDPVVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            complement(28163..28807)
                     /locus_tag="QBA20_000173"
     CDS             complement(28163..28807)
                     /locus_tag="QBA20_000173"
                     /inference="COORDINATES: protein motif:HMM:NF024911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6290860.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            complement(28807..29283)
                     /locus_tag="QBA20_000174"
     CDS             complement(28807..29283)
                     /locus_tag="QBA20_000174"
                     /inference="COORDINATES: protein motif:HMM:NF012413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDQ6290861.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETVFTQRASEEELGFSQIVYRRS"
     gene            complement(29280..29681)
                     /locus_tag="QBA20_000175"
     CDS             complement(29280..29681)
                     /locus_tag="QBA20_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290862.1"
                     /translation="MYVLCEIEGVLANTSHRKSVSDADAGQLIAGDELIFPTSRMLRG
                     FARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGVSYEKHIKRTLAEHKG
                     DLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            complement(29695..30798)
                     /locus_tag="QBA20_000176"
     CDS             complement(29695..30798)
                     /locus_tag="QBA20_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDQ6290863.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQADDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIDTFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRLGYESIYTKMLYDLEMDGSVTETELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDDILILGYDPLPWIKVPVAV"
     gene            complement(30969..31838)
                     /locus_tag="QBA20_000177"
     CDS             complement(30969..31838)
                     /locus_tag="QBA20_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HDQ6290864.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLEKKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADVEERRQAVANCKYDSSDLGFRHADGTDKMIGFRISDGKIMKSPTYSDVDLSSFVE
                     QAKASAMYGMIKK"
     gene            complement(31916..33058)
                     /gene="nrdB"
                     /locus_tag="QBA20_000178"
     CDS             complement(31916..33058)
                     /gene="nrdB"
                     /locus_tag="QBA20_000178"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDQ6290865.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWADYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            complement(33159..35474)
                     /gene="nrdA"
                     /locus_tag="QBA20_000179"
     CDS             complement(33159..35474)
                     /gene="nrdA"
                     /locus_tag="QBA20_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDQ6290866.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAT
                     SIFDGITTSQLHEALVKSAADLISPDAPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQDLFEKLYHKYEADDSIRKK
                     SVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGVRYSDTAGNKLVHETFEAIQYYLLDASCRLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEFEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEAQNPLTQAVAVEPEDECDGACKI"
     gene            complement(35548..36117)
                     /locus_tag="QBA20_000180"
     CDS             complement(35548..36117)
                     /locus_tag="QBA20_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290867.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHGLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQNSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHPKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            complement(36127..36870)
                     /locus_tag="QBA20_000181"
     CDS             complement(36127..36870)
                     /locus_tag="QBA20_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290868.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIATHETEDLN
                     IDSEGFFGAMDAHLCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAVDKRVTQENEYSDFA
                     NYPQPTLQTAIVANSLEARLLENPLWGTW"
     gene            complement(36860..38776)
                     /locus_tag="QBA20_000182"
     CDS             complement(36860..38776)
                     /locus_tag="QBA20_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6290869.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERSKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIESERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMNRARNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKENFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEVVAIESLVTRARTEVDRISKEINPFIALIARHEDNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            complement(39006..40091)
                     /locus_tag="QBA20_000183"
     CDS             complement(39006..40091)
                     /locus_tag="QBA20_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDQ6290870.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCTYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKCPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDDVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            complement(40346..40990)
                     /locus_tag="QBA20_000184"
     CDS             complement(40346..40990)
                     /locus_tag="QBA20_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290871.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAARKKPTDAL"
     gene            41734..42789
                     /gene="repA"
                     /locus_tag="QBA20_000185"
     CDS             41734..42789
                     /gene="repA"
                     /locus_tag="QBA20_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HDQ6290872.1"
                     /translation="MSENGNKNIAIVEAFSETDKKTGEVVTLVPNTNNTVQPVALMRL
                     GLFVPTLKSTSRGRKGQMVSMDASAELKQLSLAKAEGYEDIRISGLRLDMDNDFKTWV
                     GIIHAFAKHKVVGDTVTLPFVEFVRLCGIPTARSSAKLRKRLDSSLSRIATNTISFRS
                     KGSDEFYVTHLVQTAKYSVKHDTVELKADPKIFELYQFDKKVLLQLRAINELSRKESA
                     QALYTFIESLPPDPAPISLARLRARLNLTSRTITQNATVRKAMEQLREIGYLDYTEVK
                     RGNSVYFVIHYRRPKLRQAQISTKIDNDETEYSLPDENQDDIIDVVPDEKEGKMVMLS
                     KEELALLEELRKAKTRK"
     gene            complement(43278..43490)
                     /locus_tag="QBA20_000186"
     CDS             complement(43278..43490)
                     /locus_tag="QBA20_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290873.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(43490..43825)
                     /locus_tag="QBA20_000187"
     CDS             complement(43490..43825)
                     /locus_tag="QBA20_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290874.1"
                     /translation="MKAIPFTLLFLSSIVVADTTVYQCEMSVADVKNGALTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
     gene            complement(44041..44316)
                     /locus_tag="QBA20_000188"
     CDS             complement(44041..44316)
                     /locus_tag="QBA20_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290875.1"
                     /translation="MRVLVRIVTSTVYDAFPLFIVKADGLNDEETDALIQRTLVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISDEDAAHLERILSISTFE"
     gene            complement(44372..44797)
                     /locus_tag="QBA20_000189"
     CDS             complement(44372..44797)
                     /locus_tag="QBA20_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290876.1"
                     /translation="MTAIKKLYDAANVALDVIDDEVAKGFPEPDWAHQLRNAIAEMTP
                     PDPTPDETDWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPKQED"
     gene            complement(44889..45719)
                     /locus_tag="QBA20_000190"
     CDS             complement(44889..45719)
                     /locus_tag="QBA20_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDQ6290877.1"
                     /translation="MKKGLLAVALAAICTMGLTGCDRVEPGYVGIKVNKLGEDKGIGE
                     VVGVGRQWTGLNTELYVFPTFKQMKTYDEPFTFQMSDGTAIGHKIGVAYLVNRDKVTT
                     VFQTYRKGVDDITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQKEMS
                     PVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQANG
                     EADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFITVK
                     "
     gene            complement(45723..45923)
                     /locus_tag="QBA20_000191"
     CDS             complement(45723..45923)
                     /locus_tag="QBA20_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290878.1"
                     /translation="MIKIYLLAVATGLSVALIYGLLVPSLISAKSDLAVMLGVLVGFG
                     APVIGLIGGRKFINSLIKAKGK"
     gene            complement(46017..48356)
                     /locus_tag="QBA20_000192"
     CDS             complement(46017..48356)
                     /locus_tag="QBA20_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDQ6290879.1"
                     /translation="MAKGKSALALALKKKIGSNDEIQKVTHWIDTGFPPLNKAISGRY
                     DGGFPCGRIVEVFGPPSAGKCVTADTMLLTERGMVTVKELFEIEGYKATCTTRDVEHN
                     VGLINENGVIEKTSHLTWNNRRKFKRIKLASGGYIEATFRHPIRVVDDLGNVVWRHAE
                     KISVGDTIPSMVGTHQFGDQHLDANIAKLMGYLIADGYVASENSVNFSNTDPFIKDEY
                     YRLISLVSDKMPVTRKHNGSEDHVLFSKEVRSLLFKEYGLEYEKAAGKQVPLSVRRAN
                     SEAQIAFLRGYFELECHVNDGRCIEVVSASGLLLQQIRLMLLNLGITSTISEKHVAGY
                     ENTYYRLSFSGSNYDLFLSTIGFESPARLSVATKRDIGFDRTYSGYVPHISGLVKSLY
                     ESLTKTTREDYALVDHVIGRGDRVGIDKLREIYVSFIGRKNRFNEHLFAQLAAVIGSN
                     LFYDEVVAIEEGEAPTFDVAMPETHSFWSNGIISHNTFLATAAMISAQKQDGLAVFLD
                     HENSFDVGLAVANGLNADEDDGQWVYKQPDTFEDSVELIGTILKLVRDEELIPESAPI
                     CIVADSLASMVPNSKAEKFEKMAEGTAKDKDQLNMNDNTALARATSANFPTLALWARK
                     YNACIIFLNQVRTKIGVMFGDPTTSPGGDSPKFYASVRIRLGASVMKDGKEKIGQDVG
                     AECIKNKVAPPYGKCTWKFYFDPTRGLDVIESLVEYMLEEGYLPKNASGRVEIGDKKY
                     TKSQIVEMYREKPLAEIIAALQAIDDRRSKDNPTESVEE"
     gene            complement(48359..48625)
                     /locus_tag="QBA20_000193"
     CDS             complement(48359..48625)
                     /locus_tag="QBA20_000193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290880.1"
                     /translation="MLKSIINGATTTPTQLAKEIVFYHGEYAVIALPSILGAAGMKAT
                     DREFGLVSEQVVKILARVSRLLNHDAIVFDESAALKRINETKGA"
     gene            complement(48625..49569)
                     /locus_tag="QBA20_000194"
     CDS             complement(48625..49569)
                     /locus_tag="QBA20_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDQ6290881.1"
                     /translation="MNYLFVDGNSLGYYHQQSDKLHNGEMEVQAAFGFVKNVRRYASI
                     LHARPMILWDGFSDKRRDFYPDYKANRDDDPDMKKMKEGFAIQKPYILKMMTALGVTQ
                     LIAKDAEADDLAGLLVSRMAPQPTVEHIYLLTGDSDWLQLVRENVSWVSLREDAKNKQ
                     VNFEQFAELTGFATPRAFLEAKALQGDKSDNISGVGGIGAGGAKELLHEWGSVATMVR
                     GINDGSIVVDKGRHKTAFNKLAKNAFNEKTGCRMLEAFKRNITLMNLIETKFPPTEIE
                     TIKGNRDVKAFEQLCYELNFRSFLEDLEVFVLPFERYC"
     gene            complement(49630..50658)
                     /locus_tag="QBA20_000195"
     CDS             complement(49630..50658)
                     /locus_tag="QBA20_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDQ6290882.1"
                     /translation="MSKTLLDLLNKTREDIAAKRGNNVDLTRLKDGVNYIRIFPNKDD
                     PNGKFFQTFGMHYVKYQNEEGKEATNAYICEQHTHGRACQLCEMVMEGRARHKGNKAM
                     EERIGQMRATPRYLVNGILSAREDFADAEKCQLIELPSTVFDDICKAITEDIADDIGN
                     PLSKEEGYAFLIKRTGSGRDTKYDVSPKRKVYKGDIEDKFWNTQHDLIAYANQADETR
                     LLSTVRTMGRLIGIAAPTAAASAPAISSTAKTSAAALPGFGSVTGHTEGATAVATAHT
                     PASEPTSLVDEEILRAVETEFKPEASSAAVAVSVKESEAVAATSVATASATEDEGLDD
                     LLRELDSL"
     gene            complement(50776..51207)
                     /locus_tag="QBA20_000196"
     CDS             complement(50776..51207)
                     /locus_tag="QBA20_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290883.1"
                     /translation="MNTDIFSKIMADLEFDRDNLEEVWRKQPRLLMEYGSKLAQADRD
                     VAEAKLNLEAVEAKLYDTERKNLSMNGIKFNESVLDAKVKTNPQYLSKRQKLDEARHI
                     ADIYKHAVAAFSHRRDMIVQASKMAIVELERLGSERFITPR"
     gene            51747..52310
                     /locus_tag="QBA20_000197"
     CDS             51747..52310
                     /locus_tag="QBA20_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ6290884.1"
                     /translation="MALYNFTLTLSGVSYETEGLEDALYQNGCDDALICAYGNSVYVE
                     FDREAQSLDAAIASAVDNIESAGIGAIVESVDSALVGLSDIAELTDLSRQAVALLKDG
                     KRGSGDFPCPVQRIKGQSPLWDWADVAEWLMNNGRLKEGDQIVQNARTLSKWNLALRN
                     SVSKDFNEIEQIAEKLIKRRKDIAKCA"
     gene            52640..52855
                     /gene="cspG"
                     /locus_tag="QBA20_000198"
     CDS             52640..52855
                     /gene="cspG"
                     /locus_tag="QBA20_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005284516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDQ6290885.1"
                     /translation="MSNKMTGLVKWFNSDKGFGFISPADGSKDVFVHFSAIQSNNFRT
                     LEEGQQVEFSIENGAKGPAAANVVALS"
     gene            complement(53159..56677)
                     /gene="dnaE"
                     /locus_tag="QBA20_000199"
     CDS             complement(53159..56677)
                     /gene="dnaE"
                     /locus_tag="QBA20_000199"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDQ6290886.1"
                     /translation="MKALMVRTDFSLGESALKAENAVKIAREAGYTAVISADSMNIAS
                     VIPLQRAAGDDMAVICGVKLNIVDDPTYEHRAKLAKESKGCMESLERGRNYSFTALIK
                     NEKGYRDICELMTAANTREQFYFVPRLSLEQLVSTYAKGNIILLTSDIGSVFQRNDFA
                     KIISTLITAGGKDNFYSVVYPHPTPFYDQINVRAMKVASALKIEPVAFYPAYYESIDD
                     ADIKDIAHMVTNNIKIDQPHRLRIPHQRDNAINGRRHLLEALKAFSVRMDVPVTAAMA
                     STTQDSIIDACTWRWHELPPALPKMADDEPATLMKLAVAGLRKRLTTKEFGYTPPASE
                     NRVYVERLKYEMDTLTRLGFCGYFLMVRDLMNHSRETGIPVGPGRGSSAGSLVAWCIG
                     ITNVDPIRHGLLFERFINPERLDLPDADLDFSQARRHEVIEYLNERYGEDYVAGIPNF
                     TYLGAASALRDTARIYGVDAADMAVSKEFKNMEDDSLSLEELREQLASLDKYATKNPE
                     AFKAACKLQSLMRGFGRHAAGMIVAGVPLVERTPVELRGNARCIAFDKRYCEAMGLIK
                     LDVLGLATLDLLDSAKRYIKESTGEDINLDAIPLDDRKVLDGFAAGYTQGVFQLESGP
                     MRKLLKDLGGGIEPMSFKTVVATTALFRPGPIQSGMLDDYVSVAKGFMTPTSLHPVLD
                     ELTSETNGVILYQEQTMNATRLLAGFTMAEADGVRSAIGKKNMEKMRSMGEKFVIQAQ
                     AGWIDVELENGAIQRIHRAEHFKCEDGTLRTVEEALEHGVKLPMPVVRVTTSHPGLSE
                     TKAKEIWTAFEKNGAYQFNKSHSVAYSLISYQSMWLKTHYPAEFFAAALTILGEDKHQ
                     GLVKDALTYGIRVLPPDVNVSSNRIEIRTLEDGSQALYAPFSAVKGCSENGCQAIMRA
                     REKVGGKFESVAQFDEAVEKRACNSRVRESLHKVGAFASIEPGSLPATDPERLRDQAE
                     LMGNLIIDAVKASRPFEMNPKRSAEINVLMTRMAAEMGLGEELIRPTIGIKPKIMIIL
                     DNANGNDARTGYFMENGYDDFKAKLLTVGDLRMGDLYVTGVCKKVKDKEKDYTKDEIG
                     QFTDFMREEINLVRPTYILTCGSRSTALFNNKSKPSDLIGRKEYFPELDATVFYGFNP
                     NILYFRPEEGERLEAILADIAETINK"
     gene            complement(56858..58093)
                     /locus_tag="QBA20_000200"
     CDS             complement(56858..58093)
                     /locus_tag="QBA20_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6290887.1"
                     /translation="MTTTALQNENNPSDYLVCKWCGKSFHYFKSHVANGNCEGIPESV
                     KDADPDTVLKMYTTQFPDEPTLSKKALDAIQAKRAEQKSEMAKSSGVTSSPGYTGTVE
                     YKTDLVAAHELLNVTVKELGTKRGTPLMVSVNVNTPFPEFVPEVKKGYVYGDFELIKD
                     IFMMLELGIPGYLWGHAGTGKSSLPTQLCALLNRPLIRAQHTASMEEAHVTGQILARD
                     GSTYFEPGLLALAMKHGWVYLADEYDFAFPQILGVYQPVLEGEALVIKEATPEWRRIT
                     PHERFAFIGTGNTNGSGDETGLYQGTNIQNAANFSRFGIVSNVKYMSKEAEINMLINA
                     GIVDEYAEKMVKFAGIVRDGYEEHLISQPIGPRELLLSAKIGMMRGDFVTGIERSFIN
                     KLPSASAQAAREVVQKIFG"
     gene            complement(58189..60297)
                     /locus_tag="QBA20_000201"
     CDS             complement(58189..60297)
                     /locus_tag="QBA20_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HDQ6290888.1"
                     /translation="MSKKRSIKEVQDFRDSVKRVVALLSGKNIPVAERGDDAYVRYND
                     DGEPILVNIPSIPDNATPALMNAVRGFLDHEVAHILFTDIRVSNKMREKGRVPSWSLW
                     NALEDVFIERKMGQVFNGTRRNLMATQRLIIEKVFKPKASEAIAYCGKDQRALFLNFF
                     LCPVVRAWDGQAPFVDFMDEYWPVIEKPISLLKEHGIDVAVRNMSCTEDCVKVAATIA
                     KILKDTESESKGKESAPGKTSDPSDADQTDASGENNEDNEDHETPSALDNHKSIKSES
                     HSKHKHDNNDGDDSDNSESSETIFDDTENDKEVSDSDASDNAVSESFTADHEKRKTTE
                     DGSSDIPTPSKMSLEEALEELDSIEDEVGGMTEDALSETIKSELTETSKSEYRPYNRS
                     YDFIGSIDQAEAHIKRLIKTFSDIDLGGYPISRYRIVPEGNQLFDKYIEKHLSSGVSS
                     TLAKDLERAIASRNRVQFIPGQRRGRIHGSSIYRLAMNDDRVFRKKEESKAVNACVQQ
                     VIDLSGSMSGITIQLALASAYTIADALDRINVPNIITGFTTFGSHMAAGELKAVKYEF
                     SRFESLMLPIIKNWNEKVNSREVRSRMGCVGYTFPLLNNVDGESIASLASLFSGRMED
                     RKIMLVLSDGAPWAVGRGFDAHLRSVAKQIETQTDIDLMAIGIMTDAPERLYSNHALV
                     TSVDSLGSSVVTELSRIILK"
     gene            complement(60396..60608)
                     /locus_tag="QBA20_000202"
     CDS             complement(60396..60608)
                     /locus_tag="QBA20_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290889.1"
                     /translation="MNTTISSLIALEIGHVQKLADECVADILTDLPNEQIQVGVNDTT
                     GFIFELNNKRFTLLNTGSGSLAVRIC"
     gene            60860..61246
                     /locus_tag="QBA20_000203"
     CDS             60860..61246
                     /locus_tag="QBA20_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ6290890.1"
                     /translation="MSDNKIEFIESRYAAFIAGLIESSPMSQAQIAKTIGYKNANNLS
                     LIKSGKIPLPIDKVRPLALALGIEPSRLMMMVLEERQPELAAFLYKEGTAPLNEDEKQ
                     VLAAYNERFGKEKGASQKVVEAIKSL"
     gene            complement(61241..62344)
                     /locus_tag="QBA20_000204"
     CDS             complement(61241..62344)
                     /locus_tag="QBA20_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6290891.1"
                     /translation="MKNSFDRARAAENTSKEAIEYLERASQMQAVMISQVSNDMRFSD
                     AFMLFTRLSLLITRRRPEIAVHCILIHVLPHIADVKVSDINRFMVNQLVNPLILDGKI
                     VMGRRVFSLMKQFLSWCAFQGMIDVSPLNDMSLNKVAGGAKPTPRERKLTDAEVWVFW
                     NIWDYFNVCAGTKWAARLCLVSARRPDEVLRAKKSEFNLKRGVWNQGKRNKSAREHSL
                     PLSSLMRTCIEELFEYGKDSQWLVPSNKKIGKDLPMSKVAIAQALRRILERPELMELE
                     PFTPRDLRRTARSYFPALGISQEVSRKIMNHSLEGIDRVYDRHDYMDEMRDALESFST
                     YIASIVEQPDLDEIDHKFKGDRLSTELIRVNFS"
     gene            complement(62555..62800)
                     /locus_tag="QBA20_000205"
     CDS             complement(62555..62800)
                     /locus_tag="QBA20_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HDQ6290892.1"
                     /translation="MTITIYGRDNCSFCKRAVELAKQLKGHGYGDYEYIDITTAGIDK
                     EKLSEIVGKPVETIPQVLIDGQPIGGYTELAAYVSTL"
     gene            complement(62797..63147)
                     /locus_tag="QBA20_000206"
     CDS             complement(62797..63147)
                     /locus_tag="QBA20_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290893.1"
                     /translation="MTSKDNTPLKVTPEHIEQLIQSEHYFTAYDASHGDNFISVYNSK
                     TDIDKCHESLKLLTFCVMVLKNGYTVTGKSACVRPEIFNFDVGREYARKDAIDQIWPL
                     EGYLLKQKWHEAKQ"
     gene            complement(63369..63455)
                     /locus_tag="QBA20_000207"
     tRNA            complement(63369..63455)
                     /locus_tag="QBA20_000207"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63421..63423),aa:Ser,seq:tga)
     gene            complement(63820..63895)
                     /locus_tag="QBA20_000208"
     tRNA            complement(63820..63895)
                     /locus_tag="QBA20_000208"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63860..63862),aa:Thr,seq:tgt)
     gene            complement(63900..63986)
                     /locus_tag="QBA20_000209"
     tRNA            complement(63900..63986)
                     /locus_tag="QBA20_000209"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63950..63952),aa:Tyr,seq:gta)
     gene            complement(63992..64066)
                     /locus_tag="QBA20_000210"
     tRNA            complement(63992..64066)
                     /locus_tag="QBA20_000210"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64032..64034),aa:Asn,seq:gtt)
     gene            complement(64833..65123)
                     /locus_tag="QBA20_000211"
     CDS             complement(64833..65123)
                     /locus_tag="QBA20_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type natriuretic protein"
                     /protein_id="HDQ6290894.1"
                     /translation="MSNLTSWDWWLATYFLAAGVAFAFYVGQLVVKLLLIKFASHKRI
                     DDGLWRLGTLVETRYGQLKENETITIQAKRFTATITRTPSRRVALIKKVTTE"
     gene            complement(65269..65484)
                     /locus_tag="QBA20_000212"
     CDS             complement(65269..65484)
                     /locus_tag="QBA20_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290895.1"
                     /translation="MIVLSKREKETLHEISKWPEFPEYWKPKTRAKLERLGLVANVSE
                     TWCSANYQLTDKGKVLLQQLVESGALK"
     gene            complement(65468..65647)
                     /locus_tag="QBA20_000213"
     CDS             complement(65468..65647)
                     /locus_tag="QBA20_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290896.1"
                     /translation="MMLGWMIAFLAVGFLIGIVVMSSCINDYIKSGVIERRGRIYRIV
                     EITRTVKEIKDDRIK"
     gene            complement(65644..66966)
                     /locus_tag="QBA20_000214"
     CDS             complement(65644..66966)
                     /locus_tag="QBA20_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDQ6290897.1"
                     /translation="MKKLIALKHKLDEMKAMGTNAKKEALANMDDFEQSMVSLMLNPF
                     IRFGVKKYKVAEPLSESVPSDEKAIDILNKLASRELTGNAAIAAVESIVASMCADGQD
                     VFRRFLLKDPKAGVGISLCNKVFENPIPKFEVQLASPYKEKGDKYPFKPNPKAKWPMI
                     GSLKLDGLRVICEVIVDEEEVNFLSRTGNPITSLDHLKPAMLELGKLSGHKHIFFDGE
                     GTAGSFNQSVSALRKKNVQAIGAIYHVFDFFLPEWRAQAKSKEYAKTGMKLKERLAIL
                     VALFKNDRSEDYAQDIHLHPFYIIHSHEDFIERFMKRLDDNEEGEMGKDPNSVYEFKR
                     TRSWWKLKDEDSEDGEIIDFEPGDPDSGFANTLGKIVIRLENGVIVRASGIKHKYLDE
                     IWNNKEKYRGRIVEVHCHEKTPDGSLRHPRLKWPRCLRDTEDRIGDKE"
     gene            complement(66963..67220)
                     /locus_tag="QBA20_000215"
     CDS             complement(66963..67220)
                     /locus_tag="QBA20_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290898.1"
                     /translation="MMVEEIRKGESLYFEDMTALIKEVEERNKQARTIKRKRDSVTIN
                     SMVKKSTTSQTAITDVYAALLEETTKETHEQIDQHMEAKQQ"
     gene            complement(67501..68283)
                     /locus_tag="QBA20_000216"
     CDS             complement(67501..68283)
                     /locus_tag="QBA20_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290899.1"
                     /translation="MKDEIQKLACDIIDKTGLEISECNRLDIIEKAVKTAMDHIATRL
                     VEIPLPGLPYLKVKLHVWGEPSCARRYALVVFISKENPISLKVQVGAWMDGKVIYTNT
                     VFCASNDSIIEEAIQESLLTMHSLVLLEDKQNYEEYLRSIKGERTLSLKADFVTPTNL
                     LEVLLNKGANDAVNVIRESEYSTLWDMCKSQLDLVHIVIDAGKACDGVMAEFAGKVVR
                     IANELPMIEQEAKSYATNHVTELLVPYRLESNQRKMVSWGSW"
     gene            complement(68359..69474)
                     /locus_tag="QBA20_000217"
     CDS             complement(68359..69474)
                     /locus_tag="QBA20_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDQ6290900.1"
                     /translation="MSELKELITELDFEQWLDTEGIVYRRGGVSTRGREVNIKECPVC
                     GSSNWKVYFNLTSGVGKCFAGDHPEEIQFNKLVFLKHYSGKSRRQFEEYVQNALLSQG
                     WAPKKEEIVLASTVELEGPLALPRHYELPIDGRLPDYLVERNISPEMAKYFDLRYCVE
                     GKHAYVDPYTDQVKGQIFDMRILIPVYDLDGVMKTFQGRDITGTAERRYLFPMQLPAS
                     GKFLYNGHNAVGKQTVVVCEGAFDVMGVKRAIFDEETLRDYVEPIGTFGMHLSGNTTQ
                     DAEDQLGAFLTLKARGLRNVIMMWDSEKQAIRNTMAAARRLTSIGLNVKVACLGEEGL
                     DPGDATPEQIIKAYYRAKPYTKQLELQSKVLGIKALS"
     gene            complement(69622..70962)
                     /locus_tag="QBA20_000218"
     CDS             complement(69622..70962)
                     /locus_tag="QBA20_000218"
                     /inference="COORDINATES: protein motif:HMM:NF015736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6290901.1"
                     /translation="MTELCHTGRGLSEEFDEDFQNRLTAYFCRDHEFLTRAGDLVVPS
                     QFANAANAILVNMVSGYYRMYKSAPSSSAILDMLKRAKRDKTIREELFADVVAAFKRI
                     LAEKLSDTSYMVDQVSTFAKSVAFDDALIKAAELKEKGDFQGAMAIMAKVQQIGSNEA
                     TGIYDYYTSASERLKAREYEASEEYVPNSITTGLPLLDRLLYQKGWAKREMVLFMGFA
                     KSGKSTAMGEFSINATLAGYNVLYLSLEVHTTILSDRFDARLSETEMSKLVERRDEVH
                     RKLAELGATKGIGSLWVVERPSGSMSPADLDRMLNSMKANGMVPDMVVVDYADLMRAS
                     YDLRDDRANIRSIYTDLRALYDKHNVAGITASQTNREGGASEVATMMHAADNIEKVRI
                     ADLVITINKTEEEEAKGEARLYFAGSRNQQGGISIRVKQNLEQMRFIERILDVT"
     gene            complement(71006..71746)
                     /locus_tag="QBA20_000219"
     CDS             complement(71006..71746)
                     /locus_tag="QBA20_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290902.1"
                     /translation="MSQIFFDTIDNDQYDFMTEWNTAVMDKWVAENIGLSRCKDEAEL
                     FETKWFDYRDMHPLMATCLFTEAYKRQYSNIMLTHGREHFETAPFTTGLKRLPYQELS
                     TANKTSLWKARQFADRYCCSYDYFISTVLSAAARRLWDKLPRPQHLWQPELIEIFEEK
                     LARRATTRLDDSLVSFKHMGDMQFNPIQESYFEWILERLRTIPRSKRIRAIFSAIWLM
                     EIVPERLISAHFPEELEEARRFIDPLSN"
     gene            72029..72796
                     /locus_tag="QBA20_000220"
     CDS             72029..72796
                     /locus_tag="QBA20_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290903.1"
                     /translation="MDDLSVYARSLDKAKYYVYCLYDTEDKMKRPFYIGKGKSTRCLD
                     HIKYPDDSPKSMRIKELLANKKLGIDILRHGMDETTAKLVEATCIDLMGVGELTNKVR
                     GSGSMMGRISLDAYHHLVLQEETEIAPEHAGLAFLLNSTYKSGMSALALYEATRGVWA
                     KVPRDENLKYAYATYGGLIMEVYQIECWVKAGSQQYFTRDIALGPDTKRYEFVGRIAD
                     EHIRKLYVGKLIKKPPSYGSPFVKVGVVRTEHSVSAA"
     gene            complement(72849..73202)
                     /locus_tag="QBA20_000221"
     CDS             complement(72849..73202)
                     /locus_tag="QBA20_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290904.1"
                     /translation="MKMGDLAKRKEPDAPAKNTTPLRQPVRPQGRPTRGKEKIKSRTM
                     SLEDEYFELLEMMKFIPRFEKFTRSDVIRAAIFHLAEKSPQEIEDIVKLNEAITAADV
                     TMRTDEIKRELMKKG"
     gene            complement(73208..73876)
                     /locus_tag="QBA20_000222"
     CDS             complement(73208..73876)
                     /locus_tag="QBA20_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6290905.1"
                     /translation="MTKIFVVGGTKGGPGKSTLAQQIAVCLKVKKKKKVYITDIDIQR
                     TTTSWCEDRRQNEDLELIPFAYVQDDIIKHLKSLQGRAEYVVVDAGGFDSEIQRQAML
                     MADVIIIPLRPKRRDLKSLRDIDPIIDNVRNVNDKVKVRAVMNQCPALPSQVSRILAA
                     KEIVETFGIESAPVNLYNRNVYDDAEESGRSIFEMTGSERDKKAEAEFEEFVDYLLSL
                     EEEE"
     gene            74195..74464
                     /locus_tag="QBA20_000223"
     CDS             74195..74464
                     /locus_tag="QBA20_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004109769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDQ6290906.1"
                     /translation="MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERA
                     VKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKRAADRLKSME"
     gene            74472..74993
                     /locus_tag="QBA20_000224"
     CDS             74472..74993
                     /locus_tag="QBA20_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ6290907.1"
                     /translation="MSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKIL
                     RAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQKKVPYRNVPSVTLGRLALDKSL
                     QGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
                     FYPTKSIEKLFEE"
     gene            complement(75162..75413)
                     /locus_tag="QBA20_000225"
     CDS             complement(75162..75413)
                     /locus_tag="QBA20_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290908.1"
                     /translation="MLGSLMTKLKVALITLAAVLFVLVGAYTMGGNAARRAMEEKAKQ
                     EDRKRLQNTVKVVNETSSKIRQKDASAVHRELYDKWVRH"
     gene            complement(75415..76107)
                     /locus_tag="QBA20_000226"
     CDS             complement(75415..76107)
                     /locus_tag="QBA20_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290909.1"
                     /translation="MKTPRGIRNNNPGNLDKGSPWQGLVANPDEPRFCTFKDPVWGIR
                     ALAVTLITYHDKRRAKDGSSIDTIREVIERWAPPNENNTDAYINEVSKAVGVTADMII
                     DLHDYDILRPLVEAIIRHENGRGPLKTLNTWYAAEVIEEGLRRAGVVKPVKTVKAVPV
                     TKETAGATVTAGIGLAQLADVMPQVSAAMDKAQGHISSGDTVRIIFGIATIFVAGFIA
                     WSQVRRHQKGMV"
     gene            complement(76121..76444)
                     /locus_tag="QBA20_000227"
     CDS             complement(76121..76444)
                     /locus_tag="QBA20_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290910.1"
                     /translation="MSNEMAGVTTEQVERIAAIVAREVVGKLGKELREEIGQEVNDQL
                     KTYFGDMTPAQHSIQHSNLDKLLNRLDSISSGFFGGIVSKITSFIITALLLGLAAYGV
                     KNGLQ"
     gene            complement(76767..77375)
                     /locus_tag="QBA20_000228"
     CDS             complement(76767..77375)
                     /locus_tag="QBA20_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290911.1"
                     /translation="MYGSKIYRSDGKVWMSPSLSPIVFQRKQVVSLSGGTEFNTQISP
                     DRSPMIFVAYSKAVSLIANRIVRNNQVIYSFGGQGSDSSATIYVFSKGIAKKETWGMS
                     FFNAQGEEIYNTANIPLSFTFLNNTEWNSSGGHVFDYPPAIIPTYANVFAVPVPGGAM
                     TMVYGYAAYGNTVSSIFVNQLNGGHSFSVNGRVPVINRNLYN"
     gene            complement(77375..77629)
                     /locus_tag="QBA20_000229"
     CDS             complement(77375..77629)
                     /locus_tag="QBA20_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290912.1"
                     /translation="MSYGAQVWSPSRQEMVDALAPVYYLDYFTPSGSGSRTYEVEAGL
                     GIDYYIMDVTNGKYTSLTVSGNTISWSGASGNLNILVFQK"
     gene            complement(77690..79777)
                     /locus_tag="QBA20_000230"
     CDS             complement(77690..79777)
                     /locus_tag="QBA20_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HDQ6290913.1"
                     /translation="MTGTLNGNASTATKLQTARSIGGVGFDGSANINLPGVNTTGNQN
                     TTGNAATATKLQTARTIGGVSFDGTANINLPGVNTTGNQNTTGNAATATKLQTARTIN
                     GVSFDGSANISLSPANIGCPASPTGWLTTGSNGGAITTAQLVTLLQNNGAFNTKSWIA
                     RCAWAYANSATIPNSETGCGVIPLAGAVIEVFNNGSSSNNYTIRITTATTTSVSGALT
                     NAEFIYVFNGTDYSPGWRRVYNTKNKPTASDVGALPLTGGTLSGGLTSSGEIISKYAN
                     GFRIAYGSFGFFIRNDGSNTYFMLTASGDTLGSWNGLRPITINNTSGAVSIGNGLNVT
                     GGVNGSLNGNASTATKLQTARNINGVKFDGSGDININTLVSRGRVTALSGSTQGTAGI
                     QMYEAYNNSYPTTYGNVLHMKGASAAGEGELLIGWSGTSGAHAPVFIRSRRDTTDAAW
                     SAWAQVYTAKDSIPGVNTTGNQNTTGNAATATKLQTARKIAGVAFDGSADITLTAANL
                     NAYTKTEVTNLLSSYASRSSLTGYSGNLDIIAETLVVKSGGSGGFAIWDIGTTTSGAN
                     MYIDPNPGINTVWRSTSSRRYKKDIETLQDRYADELLSLRPVWYRSICRGDRKDWGYY
                     GLIAEEVGEIAPQYVHWREPTNNDSPEDISSNGMVAEGVMYERLVVPLIHHIQQLTKR
                     VEELETKLNSPKE"
     gene            complement(80303..80485)
                     /locus_tag="QBA20_000231"
                     /pseudo
     CDS             complement(80303..80485)
                     /locus_tag="QBA20_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            complement(81940..86664)
                     /locus_tag="QBA20_000232"
     CDS             complement(81940..86664)
                     /locus_tag="QBA20_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDQ6290914.1"
                     /translation="MEQFKKKKLPLLIAGAGGKKSKSSSRTPVEADDTVNSRAMAAIL
                     DLLGEGVIGGLVNGAKSIFIDDLPILNEDGSSNFSGITWDFRDGSQDQTPMAGFDFVE
                     TPKSVNIQLKKTHDVTVSIDNDEADRVRVIMKFPSLRSVDKSSGDTNGTTVKYKFQIA
                     NADATFSDVVAEGEKSVDITLTAKKTGVYYRSYELKLPKPGRAYKVRVIRLTDDNSSQ
                     YLYNDTWVDSIGEIVDTPMNYPNSALVGLKVNSEQFGSTMPSRSYLVRGIKIRVPSNY
                     NEYTNTYVGVWDGTFKLLSSSNPAWILYDLLTNTRYGLGQFVSESMIDLGQLYQIGRY
                     CDEEVDDGFGGKEKRFAINTQITSRQDAYRLIQDIAGAFRGMVFWAGGMVNIMQDSPS
                     DPVMMFTNSNVKDGLFTYKGSARKDRPSVALVTYNNKEDGYKQNIEYVEDQDAMRRYG
                     ERKTEVVAFGCTSRGQAHRVGLWLLYTARMESDVITFTAGLDASFLMPGETVLIQNKY
                     RAGKRNSGRIVEFTKNSITLDAPVSLAKSGSFIRILNQEGKIVERDVLETGENITKVT
                     FSKALSSAETPVLNGVWTITEPDLEPMRVRIVNIAQGETPGSFDITAVENNPSKYEAI
                     DNGATLIPQNTTVLDPTYSKPSNLQITEGTYLSSPGNLSVKLTATWEGKSPEYWISWR
                     RSDENNVSNWQSARVTEEQYEIVNVAENGHYDFQLYAVSFNGKKTEIISTVYQVLGTM
                     TPPDAPTSLTAVGDYRNVILNWVNPDSVDLDHINVYASQTNNLDTAKLIAESASTTFT
                     HAGLGDSETWYYWVRASNKRGMLSPPNSNLGTEATTRDVLSFLAGKITSSELGQALLE
                     DINSKASQEAVDELNEHINQSVESLEGAVNDVKEDIAELDKQFSDNLADFEIKFNERS
                     DALESAQTELKGEVSATIDKVNEAFEKIDATDAAIVEIENTVSEHDKALANTVEAIKA
                     ARDEAAALIAKESEARVEGDAANAKQLEVLQSTVEESSAAVEEMKKTVAEVDRASAEL
                     TTNIEALAKTNIDLALRQDEDQHKQMVNNAKIATTQKTFADDMSAMATKVEEIRVEIG
                     EDIKASILEESTARADGDEALAKRVTQLQSKFEGDISAAISTEQEARTSADEALTTQI
                     TQLESKVSNDIVAAIKEEQEARATEDSALASQITQLQAKVDDDISAAIRSEQEARASG
                     DSALAKQINQLQSKVDGDISAAITQEQEARASADQALSREINSLRAQTGTDIAAAVAV
                     ETKARTDADSALSTQITSLTAKANDLEASLARETTARADGDTALTKEVSSLKAQTAKD
                     ISAAVAVETQARTDADSALSSQITKLTSQYKEDIKAAVATETKTRTEQDAALATQITN
                     LESQTAANISAAVTTETTARTQADNALSGRIDTLKAEVDGNTATIQQQATAIADTNKK
                     VSTAWTLKMETSTSGGQKYVAGIALGIDTTGLSQFLVQADRFGLVNSVNGKITTPFVI
                     ENSIAYMNGAYIKDGTITNAKVGDLQSTNFVSGRSGWRFGKNGTLEINGNSGGNGRLV
                     INGQRIDVYDDNNVLRVRIGLL"
     gene            complement(86682..87272)
                     /locus_tag="QBA20_000233"
     CDS             complement(86682..87272)
                     /locus_tag="QBA20_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDQ6290915.1"
                     /translation="MSELVHVQLGGPMAKHFGRHWHLKVRNTKQALDLIEANKPGFKA
                     WMKRNIKTYDRYHIQITNKQGHKWSVDESEYQMMGQSDNIAKIRITPVPRGSGGSAFG
                     WFQTVVGAVLMVASIWVPALAPLGLSLMMGGIAQIISPQATNESVRQADNSNSYYFDG
                     PQNTTNQGNPVQLIYGEEILVGSQVVSSSITIDQLM"
     gene            complement(87260..88057)
                     /locus_tag="QBA20_000234"
     CDS             complement(87260..88057)
                     /locus_tag="QBA20_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDQ6290916.1"
                     /translation="MGNKAIPELGSDVMQQIYLCAINRYPNEACGFLVRTNGDKYRFM
                     EARNVSENPQNTFVMHVDDIMAAEDAGDVIAIWHSHTDESAEASDADRAGCEATEVPW
                     MILAIRKNVEGDAPFHFSEMNVITPDGFEMPYLGRPYVFGVFDCWMLCRDYLKREFNV
                     ELNPNAHLHIPSWYTGDNDILDQNYRNEGLVRLAPGTEPQRGDIFFIQYGKMPDHCAV
                     YIGDGMILHHQIDRLSCRAYYGGMYQKHTTHHLRHRDLLKGDETCLS"
     gene            complement(88050..88748)
                     /locus_tag="QBA20_000235"
     CDS             complement(88050..88748)
                     /locus_tag="QBA20_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDQ6290917.1"
                     /translation="MGIKADIQSLSPSALIELFVLDMSNTTSGGKLFFHAGTNELMQP
                     VVWQGVTYEPWPIKASGFDKTGQGTLPRPKIQVSNFAGTVSAEVQANDDLVGCRIIRK
                     MTLARFLDAVNFKDGNPTADPNQHFPDEMWFIEQKTLETRQVVEFELSSVFDLMGVQL
                     PYRQIIKNTCPWKYRGPECGYTGPYFDKNNQQTSMSGADYCTKRYDACNARRNYFADG
                     VIHFGGFIGATRYG"
     gene            complement(88831..89166)
                     /locus_tag="QBA20_000236"
     CDS             complement(88831..89166)
                     /locus_tag="QBA20_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6290918.1"
                     /translation="MERQTFNWYPDYESEKSVKPNVTVLNFGDDYEQRQAQGLNRIKE
                     EWSLTFTRSYNEINAIDDFLTERSGVESFYWVNPRGKQIVVVCDSHTVKRYQGYCVLT
                     ATFRQVFEA"
     gene            complement(89209..93762)
                     /locus_tag="QBA20_000237"
     CDS             complement(89209..93762)
                     /locus_tag="QBA20_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDQ6290919.1"
                     /translation="MSQNVEFILSLEDKQFTASIDRAGKLLTRFGEQVTKPAQKIQTF
                     ERSLGSVARIIGVLESKLDSTADKLQDVAAGFELASDATRKMRGNITSLNSGLKALIE
                     RVDTTTSSVDKLTASLRKVQSELNDFSDWATYASKSASRFGTEVKEASASVSGMNTRL
                     NTTTKRLSNWGVTTSQAAEGLKKVREQMDEVIGRQQLISKPVRVRTSGSGDGGSGRRS
                     GASGHSGKSNEGGMFSGLRGNIFLLGEIGDAARTVTDIMFGWQKPIVEAAAEMERMRV
                     MLRGLNKEKSNPGQAAADDMKYIVDMAQNAPFAMQALTDSFVKFRSAGLDPTDGSLKA
                     LVDSVARFGGDSELLKRAAVAVQQMSGKGVVSMEELRQQLGEAVPNAMQAMADAAGIT
                     MGELTKAVASGTVEAKQALSLMFVGLRAENENAAKDMMQTYTGALAQLQTSFTLFADR
                     VGQAGYLDSLSKGMKELASIMNSAEGISFANSLGSGLTTAIDGLRQLAQWLAKNQELV
                     INLGKVVAAMVAFKLMRAGIMGVVGAGSQMVSTFATMATAIQTPFNLGATAVTRFNRA
                     ARMGLAPIPSLIFAIRGAITGLKGAFAGLTAFIAANPIGAAFTVATVAVAGLITYMTM
                     LRSETSKVVDEIRKIPEAMTAAKRAQMADRAEKLEKQILADQRALKTGEGVNYVSNSA
                     GVITHKESKADIEARLKKNQEEYQKITGTIALGDGAVAKRLAKEAAESQIEKIRAENQ
                     IFAATFVKARQEALDKIQKINDDGSLSDDEKNKLLAPLRETVNKSYLEPAQKLVDSLS
                     SRKNATEKQIATLNDQLEKAKKDGNTEQIQKLQGSIRGYQEHLEAVAQELTQAEFERD
                     NAAKTGKGVMSNQGTVLGLGTTDKAAQKALAQYMRNQMDSATYQRTLPDGTPMMDFEG
                     KPIIGPKQLKTQLNLQKASSASSLEKMSDEERAAAIAALTKAREQDAAAAEKAAQRSA
                     NASQRAAKKEQAAQQKLAAGYQKALDKADQLMGQMGESSKATVSFDQSLRDTTKSLTD
                     LANAVPNEFITQEMIDKAKKRLEDLKNATPEYREMFNRRNVEQMISTWAPEADSIISA
                     GYTPSREEKVADFEDTYNRNLKALIELRDKASDPKVVALYTKKINQLIAAGNTALIKE
                     TGTATQKLALEYENLAEQIESTWTDLFSGLTDVLTDFVINGKMSFSSLSQSILKDITN
                     MVVKSQITLPLMNMLGMGTTAAGSSQSGNLLTGVASAVANQGVRMGNTVNGDKSVGEA
                     TKETSSSVTGLGQTTQQTTSAIGTATNAIGSWVSGLFDSTEAKDAETKAVKDSIFSMQ
                     NLSSVTGALSAAFAMLGANASGSGNKWLSFGATVASGLVSAWAGGGFDSVTSSSAKTA
                     TSSVADGTKGIPAIPKFANGGIFGKDGVIPLRAYQKGGIANSPQLALFGEGSMNEAYV
                     PLPDGRTIPVTLSTDGMSGGGNVLSPVSIEINVHSDGSTTESGDTESIWNNAAQRMKA
                     IALETIAQEKRPGGSLNPNTQRN"
     gene            complement(93770..93994)
                     /locus_tag="QBA20_000238"
     CDS             complement(93770..93994)
                     /locus_tag="QBA20_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290920.1"
                     /translation="MLSRNIDRLRAEEDVRNLQVARASQADGEAVKAFMEGLQLRIGR
                     PVVTDKVYDPSQDKADPDAKEQLMQIFGRG"
     gene            complement(94120..94437)
                     /locus_tag="QBA20_000239"
     CDS             complement(94120..94437)
                     /locus_tag="QBA20_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290921.1"
                     /translation="MTKLLDLDSILPPKKSIKFGGQEYPIVEMTVGLFVSIKQMEGKD
                     LMNMSPVEQVTAYADLVRKVIPSVPDEVLEKLTVPQLQQIFTFAMEVIDEENEKAAGE
                     GAK"
     gene            complement(94493..95239)
                     /locus_tag="QBA20_000240"
     CDS             complement(94493..95239)
                     /locus_tag="QBA20_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDQ6290922.1"
                     /translation="MSNTHVKNIKLGACKVSFGGVDLGYTKGGVQVEVATETLKVTVD
                     QLGQTVISELVQGRNITITAPLAESVLQNMVDLMPGSTLSEDDNSVTITSAQGVNLID
                     VAKELVLTPQDTTDYVLTIPKAATAGNFTMTYQSDDVRVFSVQFSAYPDDEGVLGRMS
                     GPKPVKTVSIAPESPTVKAGETVQLTAEITPADAADKTGVWESEDQEKATVDQTGLVR
                     GVAQGSTNISFTSNSGGKKATKSVTINPAD"
     gene            complement(95314..95697)
                     /locus_tag="QBA20_000241"
     CDS             complement(95314..95697)
                     /locus_tag="QBA20_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290923.1"
                     /translation="MFIEAFASLMQKAKIGTVGADIFCHYLPANVKSGVLLINPNTGI
                     SIDHELQGFYHESFTIIVRGSSITTTVEKANKIIEMFPVEETESGGVYFRLVRPMAMP
                     IIYPKNDGALIEAGIPVEFAGYLLN"
     gene            complement(95699..96172)
                     /locus_tag="QBA20_000242"
     CDS             complement(95699..96172)
                     /locus_tag="QBA20_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290924.1"
                     /translation="MGLKYDAHQFKRAGNRLNNSQKAFKRYLIRDMEKLARLVERLSR
                     AMAPLETGSLETAIFARVIKEGYSGLRIELSVSGAKPRQGHPGVEVGDYAKYMELGKY
                     RLGYLSRMKNVTNPPIAGVKPRVGPHFLERAVEISEKQFSEAILEAARKAGFTRG"
     gene            complement(96163..96507)
                     /locus_tag="QBA20_000243"
     CDS             complement(96163..96507)
                     /locus_tag="QBA20_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290925.1"
                     /translation="MNISWQTEIALYRFGAKNVYGEAQLQFVRKTNVGVVKFEQSNEK
                     SSVRADSSGSRGKASLELFDAVLVVPLEAAVQLDDVLILEGQKLKVSSVHRRWGLRGR
                     PGHLEVGANIWV"
     gene            complement(96587..97420)
                     /locus_tag="QBA20_000244"
     CDS             complement(96587..97420)
                     /locus_tag="QBA20_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290926.1"
                     /translation="MAQIDSYRSGEAVSLSFAFNVLDIESATYTVKDSTGAILVDGEP
                     LEITNGQMSIPVVVSAEYNQLSEKERDLRYVIVKAVASGLTHEERQMYVLLNSFELSI
                     PEQSFATVADAQMQAIDMLNGDTLLSDGEGLMRKRLIEATRRIKTLPFSIRKILRIDF
                     DRYDRPQNMLNVYDIPWGADGAYRHDLVDWEKMTQEKFEEFPDYFKEALMLAVVNEAC
                     EIANGNDVAAAREDGILSESIGETTNMYRTGKAANVHVARSTWRLLVSYINNRMIVRR
                     A"
     gene            complement(97420..97854)
                     /locus_tag="QBA20_000245"
     CDS             complement(97420..97854)
                     /locus_tag="QBA20_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290927.1"
                     /translation="MKPAKIVLLEPQFSGYSGMLCGVQFENGVSVAELPFIDQQRICA
                     SMRASTVEGKNVSPSAAYSDRGELTADLITEPAAPDIVPMKRGTPDEPAKQIQTFTRE
                     ELESIADNEGIAGLRVIGNQVGVKAKGIVEMIDGILKAQGGE"
     gene            complement(97899..98819)
                     /locus_tag="QBA20_000246"
     CDS             complement(97899..98819)
                     /locus_tag="QBA20_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDQ6290928.1"
                     /translation="MPEQKMKITEEAFSDFTGHMCRAGFTNSISDEPLTERQQSQLSA
                     CLQAVPLSQSVNITPASPSVLVGKTVQLSAGISMGKSASSFTWKSANDQIATVNGTGL
                     VTGVAPGKVKITATDQETQLSASVEVTVNPVAVQSVTVTPDSTSVEKGKSVSLKANVQ
                     PSNATNKAVTWSSKNEDKATVDQSGNVTGVEVGTATIEIVSQDGSKKATATVEVTAPV
                     VAVTGVEIDPNSTTVEANKTVQLTANVEPAGATNKTVTWESKNTEFATVDSETGVVTG
                     VAAGTATIEVTTQDGSHKATATVEVTAAQE"
     gene            complement(98893..99768)
                     /locus_tag="QBA20_000247"
     CDS             complement(98893..99768)
                     /locus_tag="QBA20_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDQ6290929.1"
                     /translation="MPLLREEAEKLSNNELEQGVIETIIDRDDLFAILPFMKINSKAY
                     LYNREKTLSEATFIDVNDTIPEGAATFEECVAKLRILAGDVDVDKFLATTMADTNNQL
                     AIQVRQKVKGLARAFRRNLILGDSSSNNKAFDGIPRLMHADQKIDIAGASMTFSMFDE
                     LVDAVKDLGADCIMMRSEHLRAYRALLRTVNVGPSEIMMENFGRPMLCHNGVPFIIND
                     FIPTDAGKASIYCLHLSEENGVTGLYGGDNAGIVVENIGTVQNKDAVRTRVKWYCSLA
                     NKHDKAIAALTNVKI"
     gene            complement(99794..100681)
                     /locus_tag="QBA20_000248"
     CDS             complement(99794..100681)
                     /locus_tag="QBA20_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290930.1"
                     /translation="MNLWQMLLARRGLMDVAEAHERGGAGGVAADNEQSTQDPDKQGE
                     QKEQPKGDDEYAGMTQEELLAELRKTKKAGAELLKENMKRKEKERTLADQLAQYGDID
                     PARARQLLEAEQAAENARREAEQAELERRGEFDAVKKQMIEAHQAELAQRDECYAALE
                     SENASLKSQLVEMTVGASFSNSLFLRDKVLMTPAKARVIYGSHFEVGEDGSVVGYDKP
                     AGHKERAVLVDGEGKPLPFESAIERIVRADPEADALMRSEAKQGVGSHSKSTYTISQP
                     KNKSTMDKLASGLGKIGLK"
     gene            complement(100703..102277)
                     /locus_tag="QBA20_000249"
     CDS             complement(100703..102277)
                     /locus_tag="QBA20_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDQ6290931.1"
                     /translation="MELNDKQIKDLVARRHPEYEKKKEHWDFLASTYAGGRGWFTDNI
                     FRYFKEGDQEFKERVERAYRFNHTREVVNLINKYLFKEDIHRNIEEAPEQIRNFWKRA
                     TRQNASIDSFMAAIDLQSSIYGRIWVVVDSTMSGDVESVADEKKKDARAYAYWISPQQ
                     MLDVAWDDDGNMLWALIVEVARDDADPFTSTGQEYQRYRLWTQNEWYLFREEVKKGAG
                     GAGRRQAKVILEDSGEHNLGVVPVFPVDCIGESESPYFSPSLIDDIAYLDRAVANYLS
                     NLDAIIQDQTFSQLAIPVQSLLPGDENHTKVLEMGTKRVFTYDSEGGNQPFYLSPDPK
                     QAQMIITTIKTVINEIYHSVGVAGERTKQDNAQGIDNSSGAAKMYDFQRVNSLLVTKA
                     ERLERAERQIMLLVAKWMGVDLDEDHSLIAYPESFDIRGLTDEFSVAEKLSLLQAPDS
                     VRRHQMEMLIEKIFPNITEAMKKEFDKDLLNFPPKNDLNTLENKSVLTYDRGAAQESG
                     QDQPRGNGDSSTQENE"
     gene            complement(102304..103560)
                     /locus_tag="QBA20_000250"
     CDS             complement(102304..103560)
                     /locus_tag="QBA20_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDQ6290932.1"
                     /translation="MAIPSSLSLVQLHSGQMKVFQSPHRFKVVCAGRRWGKSRLSIST
                     IIRAAAKEKKQRVWYVAPTYQMARQILWDDLQEVLPRKWVRKKNDTTMTIVLKNGSEI
                     ALKGADKPDTLRGVALHFVVLDEFQDMKADTWYKVLRPTLSSTRGGALIIGTPKGFSE
                     FHKLWTIGQNVELQRKGQWKSWQFVTADSPFVPTAEIEAAKNDMDPKSFAQEYLASFE
                     NMSGRVYYPFDRNVHVKPLQFNPRLPIWVGQDFNIDPMSSVILQPQPNGELWAIDELV
                     LFSSNTAEVCDELERRFWRWKSQITIFPDPAGAYRQHARGESDVDIFKEKGFLRVDYS
                     KKHPPIADRVNAVNRMLMTASGDIRLYIDPKCKHLIDSLEKVIYKPGTRDMDKTGGIE
                     HSADALGYPVHRRYPVKNRVILGGSR"
     gene            complement(103560..104192)
                     /locus_tag="QBA20_000251"
     CDS             complement(103560..104192)
                     /locus_tag="QBA20_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ6290933.1"
                     /translation="MTVEKDEVKTRLTPAEWAEAEAKWTSGEYTLSKLEEEYGIRRET
                     LSRHFKKRGLEKGADSVGKMVRESLKSDAELRAKARAEKIEERRTRYDGWAYALGQMV
                     MVEVTTAKREGKPLGAIEDSLKSLQRASNTLAKCFEVSSKALGMDHAENDEEEIPNLV
                     FGELTPSQVAKLRQEDDEPEIIDDELLETLEEEALSEFDATDDGSEGEDE"
     gene            complement(104388..104654)
                     /locus_tag="QBA20_000252"
     CDS             complement(104388..104654)
                     /locus_tag="QBA20_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290934.1"
                     /translation="MTLTDKQKDIIKTLNLGYERGHLLDLDELLEVLPYKTTKQSIQF
                     SIRALIKKGLVEKGHTRQRSDNRYHRRTLGLTTLGRAKAKLLVM"
     gene            complement(104664..105554)
                     /locus_tag="QBA20_000253"
     CDS             complement(104664..105554)
                     /locus_tag="QBA20_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290935.1"
                     /translation="MTDIIIKRYRPEEFPRHLDFLERMTVTKGTVEDWHALKSLHYKT
                     DGKPFAPTYYRCELDDRLVGVVVMAYPKLLLAPRHRMFPKLKPTTNTTVANQYWGRYV
                     NNNFAVISRSVVDTQYRGVGVSYRMINLVSRMHDRPIIEIQSSMSKYNPFAMKAGFKF
                     IRPERPKSYESALRVFQRHFRSDPGDNEAIVKELFAMSESRRRRALRDLVADYHKNSS
                     LAKAGRNRGTTIQDIADSLVDEASIVKLLKDIHNLSFTSPLYGVYRNPDFGRRLPDTL
                     PLLAFDKQPLDKPLEIALPA"
     gene            complement(105551..106216)
                     /locus_tag="QBA20_000254"
     CDS             complement(105551..106216)
                     /locus_tag="QBA20_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDQ6290936.1"
                     /translation="MSKYIINVSFQTRVNKTTRTLEIAESFGLGLDEKEWTLYDNLEL
                     EVKQGDVVYITGQSGSGKSVVLRELQRQMKDEGLSVASIDDFTFDNEVNVIDQLGKTT
                     SDALGLLSMAGLNDAYLFVRKPSEMSDGQKYRLKIAKLIESGAKVWAADEFGAVLDRV
                     TAQVVASNLQRAARKVGATVMVATTHEDLKNALRPDMQITKHYKERVKVEYHNGSHDE
                     VHL"
     gene            complement(106213..106881)
                     /locus_tag="QBA20_000255"
     CDS             complement(106213..106881)
                     /locus_tag="QBA20_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDQ6290937.1"
                     /translation="MTKKFEIVYRDPADLIPYEMNAKKHDEQQIRDLAAAIKKRGFDQ
                     PITVDKNDVIITGHGRREAAIFAGLERVPVIVRDDLSDDEVRAKRLEDNRLASIDYDA
                     IKLQKELESLVLDDIEVFGFEERELNVLVGSMTEEMDTDSLVIDLGEETKRQKDEHTE
                     ISREVAAEEVRVVDVLGFKTLPAGSAIVVGDLLAHMEEMTGESGVDAFVAYAEKISSG
                     EMAA"
     gene            complement(106881..107561)
                     /locus_tag="QBA20_000256"
     CDS             complement(106881..107561)
                     /locus_tag="QBA20_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDQ6290938.1"
                     /translation="MDVKITILQVDVANLRPNTWNTNSVGAQNFEKLKGSIEKLGFFK
                     PILARELEDGFFEILGGEHRWRAAIEQGISTVPVLSVGKISDVVAKQMSLVDNERYGE
                     DDQIALQRFIEEIQSELDYQLSEIAPYDDEISMVLAKEAAIDLEALEALSRGSDEPVD
                     TDKREKTERVGAEHQTMRFKVTFDASDRVAETIKNIIKEQAINTGNEMENAGEALVWL
                     VDYYKERM"
     gene            107644..109203
                     /locus_tag="QBA20_000257"
     CDS             107644..109203
                     /locus_tag="QBA20_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDQ6290939.1"
                     /translation="MTVRIVSNAVNALISGADDKVKQLVQQMLSYEVETGDWKGTSTM
                     FNWSKNSFPAGFAKPVAANLNKAGIKCVHIRKDKAPALGKPNPAVNPFPYNPDYAYQD
                     QTVETLVREGMMIAQIATGGGKSNVACKAAARIGRMTLFLTTRSVLMFQMAENFQRSI
                     DYRAKNGEPWLKDQKVGVIGSGEFQVSRHINVATVQTLASFLEEPPRDATPDKKSYHL
                     KRRELVKRFLSSVSLLILEEAHESSGSNFYDIARLCVNADYRLALTATPFMKDSTEAN
                     MRLMAVAGRIEIKVTEKYLIDRGILAKPYFLYHKVAYKPDEARIKAELANKHLNFRVG
                     MSTAYQKAYQLGIVYNLGRNEAIVREALLYKQHSLNCMTLVRLKRHGQILMEMMKESG
                     LRVDFIYGESNQTTRQAKLNSLASGEIDVLIGSTILDVGVDVPSVGAVILAGGGKAEV
                     EMRQRVGRGLRAKKNQANVCFITDFIDISNKYLLSHSYERKHIIDTTPGFAEGVLPID
                     GAFDFGVLKRD"
     gene            109206..109484
                     /locus_tag="QBA20_000258"
     CDS             109206..109484
                     /locus_tag="QBA20_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290940.1"
                     /translation="MSEKKTTYCQVALSDKANDKLGKFQVKLKEKNIKMSKAEVINTI
                     LEQLTMADFDKVISSVGASAKTREKIMRIYENSNMTKEDLETLLSRLK"
     gene            109553..109975
                     /locus_tag="QBA20_000259"
     CDS             109553..109975
                     /locus_tag="QBA20_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290941.1"
                     /translation="MKYSLLPLLTLPVLMLTACQSRPISIHDAKPAPQAKVFKYQSAA
                     PATLVVMRDTGFVGAGCDASIFINGETVAKLATGEKATFHLNAGELIVGASLEGAGLC
                     ALNPARQERETTLKNGDTKAFRVFTSNSGNIDILPTTL"
     gene            109980..110504
                     /locus_tag="QBA20_000260"
     CDS             109980..110504
                     /locus_tag="QBA20_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ6290942.1"
                     /translation="MTTKDITYGIQAELLPRDYTNVEKLLMFWRREQIPVRVTLEDGQ
                     AFCMYVYGLMPSRNKVDLCPAPFDKENRIRLPLERISTIESGVVDSIAHDFKGRLTVH
                     PDYVDNRPSRRDFFAICNQAYKANKSIRVYMADGREIEGVSAGADACQVTLRVENGRK
                     IVVLFDWVERILPF"
     gene            110508..110816
                     /locus_tag="QBA20_000261"
     CDS             110508..110816
                     /locus_tag="QBA20_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290943.1"
                     /translation="MKSILLPPLFLLCSVAANAMDYKPVIQSLMNDVCSTSQNVSVCM
                     YQFSAAVKAGKAIGENVELCKKVANEERAMLDCESSESSAQFVDALFDTNRKAVESVQ
                     "
BASE COUNT        28165 a        27212 c        24466 g        31152 t
ORIGIN      
        1 aaaaaaaaca cttgcaatct tttttcgcgt atcgataatt gaactcgtcg aaagcgaaga
       61 cgctaacgac aataaatttt aaatttacat aaggaaaaat tatcatggct aacattatca
      121 tttctaaaaa gtccatcatt gaagctgcat ctattgtatc cgacgagctg cgcgaaaaag
      181 cagatctggc aactcaaaca tataacgaac attataaaaa tggtacgcac actaaagcag
      241 acaaagcaaa tatgcaagct gcgaccacta aacttgctta cttcatcaac aacgtcgtaa
      301 acgcagtaga agacgaaaaa ttatgctctg ttttctacta tgcgattaaa gcaagcaaac
      361 aagcgccaga agtatttttc cgtgatgcaa tgactaatag ttattctctg gaaaaactgg
      421 tttatctggt taaatcaatt aaatctggta aatgcgttta ttccatcgct gatatgtcag
      481 gatctcgtgt attcgcttta atcgatatga ttaacgacga gatcgacacg ttcaccaatg
      541 gcgctgtttt cgatttaatg aatgaagcta aaaaagcgtg tgaaattaaa ttggacgctg
      601 gctatactca agccaaccag ttgatcaatc tttgcgaacg tcttggactt gttgaaaaag
      661 tcaaaggagc tgggagtgcg aaagctggta ctcagcaata tcgcttcatt aaaaatgatt
      721 tctacaatta tttagctgat gctttcaaag cgtaattaat ggaatcaagc gcccactatg
      781 ggcgctttaa aggagctttg aaaatggtca gctatgacaa gatccgcgcg gaatatcgcg
      841 caaagtatcg cgcttataaa ctggaattaa tcgatgattt aatcgcgcaa cgcgaccaat
      901 taaattttac gttttctgat ttgcttaaca gcaagcgaga ttgcaagcgc aaacgtgaat
      961 acttacgttt aagcgcatta atcggaaaac ttcaaaattc tatttaattt ttttaaggag
     1021 cttaatcatg tttgttctta tcgctggcgt taacgtccat aacgaatatt atgttaatcg
     1081 catcgctggg atcgctggtt acgctgggcg cgcagtcgag cttatagatg aaacgacgcg
     1141 caaaattgac ttattgagcg accaggagcg aaaaaaagca gacgtgaatg acgctgatat
     1201 atttttaatg ttaaaagcgt ttgtagaaat gggatttgaa atcagtttac ataaataaaa
     1261 tcgagcgccc actatgggcg cttttttcgt ttccaatact cccaccataa cgcgccatca
     1321 ttagcgcgtt ttttattgtc tttgactcac accaacaaca taaaaataag cgccaaaata
     1381 acgccataga cgcgctttta tatccttacc agtacatacc cattacttag cgcattaacg
     1441 cgcttaaatc gcgttatatt gcgttatggt gtatggttaa tcattggctt ttattcttgc
     1501 tatgtgatcc gcgtttaatt gtcggcgcgg atcggcattt tgttttgttc cgtatccgct
     1561 ctcgtattat gtgcgcgtga tttttcacat aatcacacca cttaatcaca tatgtgatta
     1621 cgctacgaaa gaaatctgga tgtctcaggc gacgaaagtc atcatgattt tccccgctca
     1681 cctgtccgac aaccgctggt tggattccac cagcttcccg atgttttttc tatataaagg
     1741 cgaatgcagc cgtttcccga aaaaatcctg gccgtttccc gtcggctcat gaatacgttc
     1801 ctcgccgttt ctgaaaattt ccctgcggca gctggtggct gtagagaaag ggccgtttct
     1861 ggccctcttc tcagttacac gccatcaagg atgtggatgc ggttgcttgc gtatacattc
     1921 agcataaagt tagcgcaaaa cagtttccat gtatcaacgc cagcggtata cgtaatgttt
     1981 ttgcatttaa tcgcattgtt ggcgatccgc atcccctgcg atactgcttc atcgtcggaa
     2041 aatttgaacg acgattgagt tttaatccag acagaaatct gcgtagcgaa ctcaatcagc
     2101 ttggactggc agaatcgccc ggagcgcacc ggaaagacga acgttccgaa tccagaattt
     2161 accacatatg ctttctcaaa tacccgcgag taacgacgat tgccaatgat gtcgcgtgca
     2221 ataatgcatt tttcttgtgc tgacagttct accgtctcat tgtcgcgcca tgcaccaagt
     2281 actcgttttt caatgtcaga gaacgttaca gcgatattgc catgtgcggg tgcgtttaca
     2341 gtagcgataa aattcatgat taattcctta tcgtaaataa caaattgttt tcttgttggt
     2401 gttaattatc gttgtacgaa taaggcgtca aagtggaaag ttgcggtagc cggacgggaa
     2461 caggtgggtt tgtcggttgc ctggaggtaa gaggttggtg tttttagcct gcgggaaaca
     2521 ggatggtcat ttaaggccac cagcattggt ggccttaatc tttagtggag cagaccgatg
     2581 tcgatggtat cccctgaatc atccacacgg atcatcagca tggcaaaggc gtttagtgga
     2641 tagcttgcgt gccattcagg gaagcggtca tctcgcatga agtcggcaat gtcgtaaacg
     2701 catccctcaa agtggaagaa tcgcgtgctt acctgctcgt cataatccac atgatccatt
     2761 tcttgctgtt ctgtttccgg caagtccagc catgattcaa ggaatacatt ttgtgcttta
     2821 ggggtaatgg tgaaatcagt catgagcata tcctcactgc gtaaacatgt tgttttcttg
     2881 ttggtgtaat tatcgcagtg tagataaggc ataaaagaag ttaatacagg ctgaatacac
     2941 aagaaaggtt ctggaagttg atagccaggt ttcaaactaa ccttcggtta ttaaaagtca
     3001 tttaagacca ccaatgctgg tggccttaat aattatcgcc cgattacgct aaggatcttt
     3061 tcctcaactg atttgttgtt gcgactaaac tgccttgcgt atctgatgac agaagaggcg
     3121 tttcgctttc tgaccatctc ggctctctcc ttcaggcgtt tcttgaaatc gcccatattt
     3181 accaccaggc gcagaaggtc agtctgttat tgtgaaagtc gtggttctta atcagatctt
     3241 ccacaagctc tttcaactcc cccacatcat gccagtaacc ttcatcgtac tcctgactgc
     3301 cgaagaaaaa accttcccgg gtaggcaagt actcttcgca attactttcg tttatgtgca
     3361 tcaaatgagc cttcagaaaa caaatgtcat tcatcgttaa ttctaaaagc tcacaatttt
     3421 cgacttcacc tacgttgcgc tccatccacc caacgagcgc attgaactta cggaagtaag
     3481 caacccgctt tctggatgcc tcgttattca gatcgttttt aggctgtgtc tcgatataga
     3541 tatcaagtcc catgatggtt tccttattaa gttgcttcag tgaaatcatt ttcacaaatc
     3601 agataaggca gaaaacaaat tgttatcggg cttaataaaa tggcgcggtt tacgcgccat
     3661 ttaaagagga ttaagcgaat acgctttcag ggattaaggt gtcggcagga atgccagagg
     3721 taatgcgcag accgtactgg ccaatccagg tggtgctggt gttcaggctg gaggcgaata
     3781 cggtgttaac catattcatc atggtttcaa acacctcttc atcaacctgg cggaagtagt
     3841 tctcgatttt cagcagcagc gggtcagttg catcgagaat ggactggtat tgagtcgcgt
     3901 actcgccgcc ggaggcatca cctttacgca cgatcgtact ggcctgtact tcggcaccag
     3961 tttcggtgtt gtacgtaaca acggtgattt ttgccacatt cttaccttcg gcggtttcag
     4021 aggcatagta aacatcaact gtcagttttt cgcgtttaac ggtcatttca ctctccgtag
     4081 tgtattggtt atttttatat tctatgatcg taagtatgta cttacaatac aaaaaagccc
     4141 cgtaaggatg gcggggctgt cgtactgtcg actaattacc acttgcacat tatcaggcta
     4201 ttgccatcac tgatgctctc tgaatttctt gctgggccac gcggttaact tccatcaaag
     4261 ccagttcgag ctgatgttcc ggccacattc tttgaaatgc catccagcct tttccacgct
     4321 ggcgacgaac attcattaca taacgagtaa aggtgttttt atcgaaagac accgctgttt
     4381 cacggaacag acgaatagag gttccattgg caacgatgtc cagcaaagtt aattgagcaa
     4441 ccagcgcagg cttctctttt ctcttctggt ctttgtccag accaagaacc aatgattttt
     4501 tcatcacaac tccgtaaaca acttgttttc tcaatggcgt aaataatacc aataagaaaa
     4561 cggctacaaa gcataatgat caggtgcatg agcgatatgc aggttcttcc ctgcggctat
     4621 agagaaatta agccagcaat gctggctttg gttgggatta cgcgaatacc tcaagaaact
     4681 cctgaacgtc ccaatgctgg acgaggtgaa gatagacctt agaaatcacg tcattttcgc
     4741 tatcgcatgt gaagtagtac ttctggctgc ggttgtatgt gtccacacaa aattcgctca
     4801 tatccataaa gtcagcatct tcacccaggt cgctgatcat caaagcaggt gcatcatcac
     4861 gagtcatcag ctccagcacc catcttgaat taatcagagt ggaagaccaa ccttccagct
     4921 catagaactg gtcaaactct ttttgagtta atttcttgat ttggttgatg tcgatatttg
     4981 caaacataaa gcaatcctta aacaacatgt tttcttgatg gtgttattat cgcaataacg
     5041 cacaggcgaa aaaggatttt attccggtga atgtggtttt ggcagtgagt tttcaggttc
     5101 agtaccgaga acacttttta cattattctg atttagtggc cgaaggtggc tggtgggcag
     5161 aataatcatc tccccgaaaa cctactgaaa tcgattgtga cccgttggct gtctggcaca
     5221 attacgctgt aactgggcat tcagagggag tccaacggta agttagtccg cctgcacgtg
     5281 agcgcctctc ttcttcccgc acattacagg tggatagccg caactctccc tctcgctata
     5341 ccggtactcc accgttcctg aaccggcatc gagccttttc ccgaaatcca cacaggcaac
     5401 tcgaccgttt ccctgaaaac ctccagccgt tccccgaagg caacccagcc gtttctgtag
     5461 cgggatgcaa ccttttccct gtatggatcg aaggctgacc gtaggggtgt tacgggggat
     5521 aaaatcagga gttgaataaa tacgaaaaag gaggcgacac ccctccttcc ctccccactt
     5581 atacatacct gtcttgttcg ctatacatgt tgtgttttct tcgtgtttta tctgtctcaa
     5641 cctctctata ggcgttaatg ctcacccctt ccagaacgtt ctatgcggcg actatttctg
     5701 tttgtgaatt gggtgtgttg ttctctctgt cggtgtatgt ggtttcgttc gttttgcggt
     5761 ggttcttcta tggggtgttc tcgtcgtttt ggtgattgtt cttctccgtg tatggagtaa
     5821 tggcaggtgg gcgattcctt tcgttattgt gtttgagttg tttgtctgga gtgtgtgaaa
     5881 ggcttttgta gttattcgtg tattgggtaa tggcgtttcc cgtgattgtt atgtttcgtg
     5941 tcttttggga ttgagagaaa ccttgcggtt acaggcttcg ttggggtttc agtgattctg
     6001 gtgtttcttc gctgtttggg taatggcgtg tcgtgggtat cgcgcgtatg aatgttttcg
     6061 gattgcctgt gaaagttagg ttggttgtcc ggtagcctgg ggagaagagg tgggtctttt
     6121 cggtagcctg ccataaagag gttggttgtt ttggggaatg gcatgtgtgt tatttagttg
     6181 ttttgttatg cctgaaaata agttgtttat atggttgtgt aacgcaacgg gagcgatttt
     6241 gagcgtgtgt ttttgttggt agtgtgttgg gtcgtctgtg gtgaaaattg gacgctgtgg
     6301 cgctgtctgc tgcgtagaat tacgggtata agaagtgatg tgtgaatacg tcaatttttt
     6361 agaccaaatc gggtgaaagc gttgatgttt cgttgatctg ttattcagtt gttttcttga
     6421 ttgtataaac aactataact tattgatatt taaaggtgtg attgcaggta tggaagaaga
     6481 gtagtggcga tcaacggatt cttatagaaa agaatcaaat tgtgaccgcc attatcaact
     6541 gattgattaa gctaattaca ctcaaacatc cccgttctta atccactgcc tgacttgctc
     6601 gtcttgtgcg acttctttct catgtttggc aatatacaag tctggcaata ggggtggagc
     6661 gaattcaggc tctgtcgcac gccacttttc atatgcgtca cgcatggatt tctcaacatt
     6721 ccaatgctcc aactcttggt attcaatcgg ccagttatcc tgcaatctct tcactgcatt
     6781 agccaggtcg tatcggaagt aaggattaag accaatgaaa ttaccagcca gcatgtcatc
     6841 aacctccttc actctggcaa tcttagaaaa cttaccaata gcaatgttga ggtcccaatc
     6901 tgacacgaac aacaaaagtg ctgcaaatgc cagaaactta gcttccccgt atgctctgtc
     6961 gctaaccgtt tttgtctgaa actcaatgta attgcccatc acaaaactag taagcttata
     7021 ggtcttactt atctcgtcct ccaaatgcga tggatcgaga attgtgcggt cacgattatt
     7081 gtttatgtgt tctaagatta cagcaatgtc tctatgtgag actgaaattt cacgcaattg
     7141 ctggagagtc ttgatgtgaa ctcttagcac ctctttgtca atcgcacgct tccaagaaaa
     7201 accaggcaca gtaacctgaa tgggcgcgaa aggtagaata tcaggagttg tccatgctag
     7261 atatgcatca cgtaacccac taatataata aagccagtct aaatgctcgc tttctaacca
     7321 tatttcaaat gtggaatcct ttcgcttatt gaagaaccat tccctcatct gccacatttt
     7381 ttcttgagta aatatccctt cctgtatgcc ggcacagacc agctttcgta ttacctgcga
     7441 atactttgtt cgatcttttg tgaatgagac aaggtgctgc ttcacctcag atgttagtct
     7501 atctccatag aaatcacatg atggatgatg gccttgacca tcttcaccgg taaatctgaa
     7561 atgcgccttc ttgtgatagc caccattcct agtggctcta acgaacgaac cacctccaac
     7621 tttgcaaatg gggcagatga tatcgtcacg ggcaatagca tgaaccgagc tgtcatctaa
     7681 attctttagc gccaagtact gctcaggatc gacttctttt tgtaacgatt ttgaaaaagc
     7741 ggtatcagtc attccaagct ccgagcaagg ggttatttgt tcaattatca ttcggaatta
     7801 tttactgtct cgcccagttt cacgtcattt tattccctag tctagctttt ctagggtata
     7861 cagcttatta caagctcctc attattaact gtttccgtaa ctcattgatc cgcttataca
     7921 ctctaccaac accagcactc aagcccttca acgatcggcg caccacaatc aaaatgtaca
     7981 gagtcaaagc ctacatcaag tactttctga atgttagcaa tcgcgccttc ggatatacca
     8041 tatcgacgca agtcgtcttt ccagacatca tcctgtaacc cggcgtggac aatacaaccg
     8101 ccatcaattt gtgaaaccca atccagctca ttgcttgcga tcatgaatcg gcgagcatct
     8161 tcctctgatg tatgagatga actgatcaat gccacctggt aggcttgggt aatgttcaac
     8221 atttcaacca ctccgaaaca atttgttttc ttatgtggct tattatctca atcaacaaaa
     8281 gatagaaaac aaattgtttt agggcttgtg taaatcagga gggattgtgt ctatatgaaa
     8341 atgcccagcg tgttgccggg cattgcgaac agaggttgct taattactta acctggtggc
     8401 caggtttagc accagcatcc gggcttaaca ggaagatatc tttcccgccg gggccagcag
     8461 ccatcaccat accttcggag atgccgaagc gcatcttacg cggagccagg ttagccacca
     8521 taatggtgtg acgaccaatc agtgcctgtg ggtctggata agcagaacgg atgcctgaga
     8581 agacattgcg tttttcaccg ccgagatcca gcgtcaggcg cagcagtttg tcagaacctt
     8641 caacaaactc tgcgttttcg atcagtgcca cgcgcaggtc aactttggca aagtcgtcaa
     8701 aggtgatggt ttcctgaatc gggtcgtctg ccagcgggcc agtaactggc gcggtagtag
     8761 ctttcacttc ttctttagac gcttccacca atgcttccac ctgtttcatg tcgatacggt
     8821 tatacagtgc tttgaacgga ttcactttat ggcccagcag cggttgctgg atgccatccc
     8881 aggtcagttc cgtattcagg aatgcttccg cacgttctgt cagtttcggc agtaccggtt
     8941 tcaggtaagt catcagcacg cggaacaggt tgatgcccat tgagcaaatc gcctgcaggt
     9001 cagcatcgcg accttcctgt tttgcgacta cccaaggagc ctgctcgtcg acatagcggt
     9061 tagccaggtc agccagtgcc atgatttcac ggatagcttt accaaattcg cggctttccc
     9121 acgcttcacc aatcacttca gcggcatcag tgaaggtttt gtacaactgc gggtcagcca
     9181 gctcgcttgc cagcacaccg tcaaaacgct tattgataaa gccagcgtta cgggacgcca
     9241 ggttaaccac tttgttcacg atatcggcat tcacacgctg aacgaaatcc tccaggttga
     9301 gatcgatatc atcaatgcgc gaagagagtt tcgcagtgta gtagtaacgc aggctgtcag
     9361 cgtcaaaatg attcaaccag gtgctggctt taataaaggt gccgcgagac ttggacatct
     9421 tcgcgccgtt caccgtcaca tagccgtgaa caaacaggtt ggtcggcttg cggaagttgc
     9481 tgccttccag catggcaggc cagaacaggc tgtggaagta aacgatatct ttaccgatga
     9541 agtggtacag ctcggcggtg gagtctttct tccagtattc atcgaagctg gtagtgtcgc
     9601 cgcgcttgtc gcacagattc ttgaaggaac ccatgtagcc aatcggtgcg tccagccaga
     9661 cgtagaaata tttacccggc gcgttcggaa tttcaaaacc gaagtaaggt gcgtcgcggg
     9721 agatatccca ctgttgcaga ccagattcaa accactcctg cattttgttt gccacctgct
     9781 cctgcaacgc accgctgcgg gtccatgcct gcaacatttc gctgaaagag ggcagatcaa
     9841 agaagaagtg ttcagaatca cgcattaccg gggtagcacc agaaaccacc gatttcggct
     9901 cgatcagttc agtcgggctg taggtcgcgc cgcaaacttc acagttatcg ccgtattgat
     9961 ctggcgcttt acatttcggg caggtgcctt tcacaaaacg atccggcagg aacatgcctt
    10021 tttccgggtc gtacaactga gagatagtgc gatttttaat aaaaccgttc tctttcaggc
    10081 gagtatagat aagctcagac aactgacggt tctcttcgct gtgcgtcgag tgatagttgt
    10141 catagctgat gttaaagcct gcgaaatcag tctggtgttc ctgactcatt tcgccaatca
    10201 tctgctccgg tgtgatacca agttgctgtg ctttcagcat gatcggcgta ccgtgggcat
    10261 cgtcggcaca gatgaaatta acctcgtggc cgcgcattcg ctggtaacga acccagacat
    10321 cagcctggat gtgctccagc atatggccga ggtggattga gccgttagcg tacggcagcg
    10381 cgcacgtcac cagaattttc ttcgcgactt gggtcatagt aggcattact tctttgtagt
    10441 gaaaaggggc ttgatagtaa caaaatggcc ttatgtctgc catgtgataa cagcatttct
    10501 cataaatgat taaatgtcgc agctggagta cactacaagg cgacaattgc gcaaattaaa
    10561 ataaaggaga cgggatgagc gagtccaaat cgccggatgc cctgagagca atggtagccg
    10621 gtacgctggc taattttcag cacccaaccc tgaaacacaa cctgactacg cttaaagcgt
    10681 tacaccatgt tgcgttgatg gatgacacac tgcatgtcga actaatcatg cctttcgtgt
    10741 ggaataagcc ttttgaagac ctgaaagagc aatgtagtgg tgatctgctc cgtatcactg
    10801 gcgcaaaggc tattgactgg aagctgtcgt acaacattgc cacgcttaag cgcgtcaaaa
    10861 accaaccagg cattaatggt gttaagaata ttatcgccgt aagctcaggc aagggcggtg
    10921 taggcaaatc gtccacagca gtaaacttgg cactggcttt ggctgctgaa ggtgcgaaag
    10981 ttggtatttt ggatgccgat atctatggtc catcaattcc aaccatgctg ggcgcggaaa
    11041 accaacgtcc aacctcaccg gacggtactc acatggcacc tatcatgtct catggcctgg
    11101 caaccaactc tattggttat ctggtcaccg acgacaatgc aatggtgtgg cgtggaccga
    11161 tggccagcaa ggcgctgatg cagatgttgc aggaaacact gtggcctgat ctggactatc
    11221 tcgttctcga tatgccgccg ggcactggtg atattcaatt gacgctggcg cagaacattc
    11281 ctgtaactgg tgcggttgtg gtaactacgc cgcaagacat cgcgctgatc gatgcgaaga
    11341 aaggcattgt gatgttcgaa aaagtcgaag tgccggtact aggtatcgtc gaaaacatga
    11401 gtgtgcatat ttgcagtaac tgcggtcatc acgagccaat ttttggcacc ggtggcgcac
    11461 agaaactggc cgagaaatac aacacgcagt tgttaggcca aatgccactt catatctctc
    11521 ttcgtgaaga tctggataac ggaacaccaa ccgttattag tcgtccagat agcgaattta
    11581 cggctattta tcgtgacctc gcagatcgcg ttgctgccca aatgtattgg caaggtgaag
    11641 taatacctgg cgaaatcgca ttccgcgcag tgtgataaaa aggtggtgca atgcaccacc
    11701 taaacagttc tcctacatca caggacaatc atcaaactcg ccacttcttg cgtcgttgat
    11761 aatgtgagtg atcacaccaa acacggcatt gctgcccgtg catccatcat catcctttgg
    11821 caatgcttcc ttcttaccag ttcttaaatc ctccagatgc tgacgtggat acttccggta
    11881 tctttttacg cgatattccc catccagagc acatacaaga agagatccat caacaggagt
    11941 cagtgaggaa tcgaccacca gcaatgcacc ctgcaatatt ccctcacgat gatgactatc
    12001 agccgccctc atgaagtagg tcgctgaagg atgtctaatt atctgctgat caagagaaat
    12061 acggctctca gcataatccg ccgcaggaga agggaagccc ataatactcg cctcttaatg
    12121 ctgtatatgc atacagtata tgttgaagct atatattttt gaactctatc tcttaaaaaa
    12181 gatgttaata agatgctaac tattgaaagg cataaatata tacaattcca acaaacgtta
    12241 tttttaacaa atttttactt cccattgact ttcccagact ccttgtctga actaatgtcc
    12301 gcggaaacca ctttttcctt tacgagttat ccacaaagtt atgcacttgc acatagtctc
    12361 tataaatcct atcttaattc gcaagacact gatgttgcgg tattgtgggt acatttggtt
    12421 aaaaacggac attaaaaaag cccagttgac taggctgggc tttttaaaag gaaacagagt
    12481 ttgcgcctct gttcctgagc cataagtatc tggcgactga gctaagatta accgaatgta
    12541 ctggattcat caagaaacgt tgagccgtca tgctaggtaa taatgaccat ttaccaaaca
    12601 tcgttgttct caaagaagat ttaagtccag gtttttggtg actctaaaac cttaccttaa
    12661 ctcgaaatcg tgccgtaagt atctggcgac tacagatatt gcaactgcaa aggcaagctc
    12721 aaaggtattt gcagctaggt gcgataaaac taagtgaggc ctatagtgct taacttactt
    12781 ttcaaaatcg tgccgcctat gttcgcaatc atcgaagcga tcattgagta cgtcaaccag
    12841 cgcccataat gctagttgtt accggcccca tccaaatggg gccattttat tgtattgact
    12901 acatactttc tgatctaatt gagttgttca ctgctctggc aattttctcc cgcaattgct
    12961 gcgttccgtg gtactgaacc gcaatatccc gcaactcatt caccagttct ctaatctggt
    13021 gatctttaag ctcattattt gggtgatggg caacaactct ggttaattca cccttgtctt
    13081 ttgctcttac gtatgcagct cttgtgcatt cgacccatgt attaccgcct acattaactt
    13141 cgtagattag gctggactta tctgtcaatt cggaacaaat acgggccgtg gtctggcaac
    13201 tagagcattc gcagttatcg cggaaaccgt gatctaccgg tcgagaaaga ggtttaactt
    13261 tttcatccaa cttcatcaca gcatcgtgat aagcgttcca gccgtcatct ttaccaagtt
    13321 gataaatctc tgaaggctcc atgtagtcta tatcgcaacc ttcttcatct gatctatctg
    13381 gtcgtttccc tggtaatcgt tcataaaaac gatttctgtc tcgacgttcg gctctcagtg
    13441 ctaacgcaag aagttgctca agcgaggtat ttgcaccagc aataatttca ttgagaatat
    13501 cttctgtgag atctttcgct accgtgctca tttatctctc tcagtatgtt gcgatggcgt
    13561 aaatttgacg acatcaaata ttttggatgg catcaacttt atccgcgagc ttagttcggc
    13621 tcttctctgt aatgcggtta ataatgtttt cgttctgacg ccttcgtgta tcctcatccc
    13681 tggcacaaga actacatcgc aggggagatg ctcaggtgac aaatcatttt gggcatcgct
    13741 aatcagtcgt gtaatctctc tttcaagccg accgcctagc tctgttttag tgcaatgttc
    13801 tgaccaatcg ccagcctcaa gcagcgcgag aatgcttaaa atatcgtcca atagatcagt
    13861 ggtattatcg acttccttct gaaactcacc ggctactgat ttatgcatca ttcgtcatcc
    13921 tcatccgagt catcctcatc acaggatgag agtagtgggt tagtcattcg ccctacttga
    13981 gtggcgtagc cacgccgaca gagattgcgc agcacactgt atatttcgaa catctcggtt
    14041 cgttcatcgc caacatcaag ctcagatgct atagcgtggc attcagtcgc gagagccgat
    14101 attttctgaa ataactctgc attattcact gttcgactcc tgcggcgttc tggtagtggc
    14161 atccagtgtg tgacattgct aatcagacca tattcattag tttgaggatg gttgccgtta
    14221 tcgtctccgt atttaagact ctccataaag ccataatgcc tatcaccatt aacgctcaca
    14281 aagccgtaat aagcaggtat aacgccgatc tcacacgtaa ccagtaaagg aaaactagtt
    14341 ctccaattta gctcgccaat tacaggcatt cgctcactac agcttatcca accatcctta
    14401 gatgcggctg tattggcata ggccttacgg tgcttctgta gctctgctgc aatcgctctc
    14461 acgacttcaa ctggtgccct tgcagcaaac tcaatgttgg tgatcagctc attaagatat
    14521 ttctcgcttg ggtagcattt cttattgtta acggtggtca ttaggcagct ctctcaacaa
    14581 caagcaattc gtcgtagtca taatcagttt cggaaccatc cgtaccgaac agagtcactt
    14641 tgtcattttc catccaaaaa gatttaaccg cacattgttg acctttagat gttatgagca
    14701 catcaccagg gattacgtct tcagcgcgaa cctctagtgt tctcatcgtg ctgccccctc
    14761 tttggtgaaa atgcctgctg caatgctgtt tatgatgctg tcagtgcatg gagtagaaag
    14821 ctgggcatct ccagcaattt tcatgacctc aacatccgca tatcgaatac cgaggtgtat
    14881 cagaccggct atacctgact taagtcgaac attttccata aacagatctt tttcccgctg
    14941 tttagcggct tccaactcca tcgacaattt ttccaactgc tctttatgct tcttgtattc
    15001 ctgatacgcg tgccaagact gacctttgcg cacactatca gcgatatcag caacctgctc
    15061 tggtgttagc gtggtcagtg gctgtgatgg aaaaatcatc acttgcccgg catcccaatc
    15121 aaaacccgcc tgaattgact gaacctcaac tgatggtgtt gaaccgatgc gaccaggcga
    15181 atgaacaacg atcgttacat ccatatcgcg acgatggctg tggttgttgg acaaaatacg
    15241 attcaccaac tcagaaaatt tggataattt catgctgatc tctctttctc tccccggata
    15301 attctgttgc acacatccac acactcgtcg cagatgaaaa cggcgtcccc agcaattaac
    15361 tttttgacgg cgtactggga tttgttgcag aagctgcaac aaagtgttcg cctgactgat
    15421 aaggatggag cgccagatgt cagccgctcc atatcgttct tgcgacgcaa tatcacacgg
    15481 ctgaactcaa ccagtttctc cggggatatc tcctcctccg tagcgagcgt ctctagtcgt
    15541 tcgagtatgt tgaaggcttt ttcttgggta atagcagagt gcgatgtggt cattttcttt
    15601 caccttcaat gttcgtggcg ctggcaagat tgtccttgcg aagaagagcg gctatgcgct
    15661 tacttccatc cgagataacg ccctcgtaat actcacgctg ctcgttgagt tttgattttg
    15721 ccaactccag ttgttttgtt agttccgcaa tacggcaaac atcgttgata cgcgtttcct
    15781 ctaatgcgtt gatctcatcc agtagtgcca gcacggtttc tggtccggcc agaaatttga
    15841 aggcgttgag ggcatcaata tcaacaccgc tatctttaag ttcctgttcg catatcagat
    15901 catcatcaac tggcaacatt aacaggcgtt ccattgccgg aattgcacgc tctgccgcct
    15961 cacgcagtgc ctgatagtca atcttgctca ctggatgact cctttacgaa gctgttcagc
    16021 aatacttacg catatctctg cgcctctaat cagccccgga acgttcttgt ttggcccaac
    16081 ttcaccatca acaaaatcaa tcatcgcgtt acgagccata tccacgccct gcgcacgtac
    16141 ttcagccaga aaagcatcgg tggctggagt ctccgttaat tcgctaaccc aattatctcc
    16201 gtgttcctcg gcacacatcg catcgaatgc tcgctcagat tctttcaggg ctgcattctc
    16261 cgccgctaat gcgtctctct gcgctcgcaa cttctcaatt tcggcagcca tgtagtaacc
    16321 aaccatagcg aaagtggcga aagggtagtc agaatcatca ggagatacgg aggccattag
    16381 cagaccatcg tgcatgttct ggcttccatc agtgatggat accgcataag aatcgctggt
    16441 ttcccgttta tacaatacga cgaccgggtt cttgattttc tctctcatta cgatgcctct
    16501 aacttatcta actctttcag cccttcacgt accgcgttca caatgcgttc cagatactgg
    16561 tatttcgggt ttggtaccgt tggccagtcg gcataccacg gatcatcacc aaagagattc
    16621 agcagtttgt taccgacgcc gaaacaacag cagctttctt ttacgtcatc ggtgttttcc
    16681 gcctcgtccc acatttcacg agccagtacg gcgtcgattt ctctctctct tcgtaacttt
    16741 atgatttctg acttcacgaa aagcagattt gcatcgttgt catcgtcgac cgtgcttcgc
    16801 agttgagagt cgaaatagcc gattagatac tcgttgctga cccgcttaat gaacgtctgc
    16861 acatcatcac cgcccatagc aaaccaagcc gcagtccacg cttttccgta gcagatgatg
    16921 gtgattcttc ccttaccagg ttcgtagttt tcaatcatca ctcgaaccgg atcgaggcgt
    16981 tccagatccg tcagcacaaa ggaaagaaca tctatttttt cgaccttcat gcagcctccc
    17041 acgcgctcca gatcagcgat ttgtgttttt cataacgttt gatgtgtacc ggtttcagaa
    17101 gcgcaatacc ttcagtgact ttcgccattg cgcttaatag caacgggtgt tctataccta
    17161 tcctctccat gtactcgaca acatcagcgt tggcaccaat ctctttgccg tgaaagagta
    17221 cgtccagcat cagttctttc tctgcgaggt agacagctga aaactcggga tcgcgactca
    17281 cgatagaaga ggtattgtcg agcaggtcag ccagcttgat catgcgcgtc tgcatgtcga
    17341 gattttgctc cagctcccgg acattgatga tgaaacgctg tattcgattg ccgtcttctg
    17401 gccttgcaat gttggtcaga gcctgaacca tttcagctac gcgctcaccg aagtgatcac
    17461 gaaccatctc tatggttaca tgggtgtctt cgaccacatc atgcagaagc gccgcgattt
    17521 gcatctccac ggttccgtca tgcaatgcaa caatctctcg gacagcaacc gggtggttga
    17581 tgtagtcttc gccggtatat tttcttttct gaccaacgcc accatgagcg ccagcggcga
    17641 acatatgagc ttcggaaata ctagacattc ttaactccgt tgttttctag ttgttgttat
    17701 tttcacaaat aagaaaaggt agaaaacaat ttatttaagg ccacaactta tggcctttat
    17761 ttattcatgc cgaaggattt gtagcgtcta attgccggat aaaatggggt gaggtgcttg
    17821 ccagtaggct ccaccggtcc atgtgaaccc catgcgaata agagtgttta ttgcggcttt
    17881 ggtagactcc gattcgacac tggcttttcg aaactcttga acctctttcg ccaacttgta
    17941 tgtaattgtg caggggacga ttgcagccca gcctttctca tgactgttaa tgaccatctg
    18001 gatgtgacca tccagaatag gttgattagg catgttattg aaaatgtctg cctgtttttg
    18061 aatgtgaaga aaaagccttg ccagctccat ttgctcacta cgagacaagg gattatcaag
    18121 agggcttttt gcaaaagcgg ctatacgctg tgcgtcaatg ccaaacatca gcgcacctca
    18181 ccatctttat ggctggagtt aacttccgcc attaaatgtc tgacgtaatc gacaagagag
    18241 ccacctggtg gaatctggca ttcctcaact aactggaagt agatatccgc tgcattacgt
    18301 gtattgctac ccgcacatat tctttcttcc cgaagtgcat gaagctcgtt gatcaaacgg
    18361 tcacactctc cgttacgctg atccacaacg gcctcaagct ctgcgacgcg ttcacctggc
    18421 gtcttacctt ctttgcgttg gatggtaacg acgaaatcgc caattgcagg gacgttgtaa
    18481 tccagctcaa ggtagttttt ggcaccgctg cggacgaact cacccgcgaa catggtggcg
    18541 aacatcgcag aggccacttc gccgttgaaa agtgactcca gatctaacgg agtcccggca
    18601 tcaagagcct cttttgctgt atccattgca tccatgaacc gatcaaactc attggcgcgc
    18661 ttttccaggt ttttccattg ctcgctccaa cgtttggcaa cgaagtctat gaatgttttc
    18721 gcggattcat caaaatcccc ctcaaactta acgatgcctt tatcaataat gatctggcct
    18781 accgcatcct ctgcataacc tttcaatgta aacttgaaag gaaaatcaga atggcctgtg
    18841 acgttaaaat gctctaactg gaggttgttc atgtgttttt ctcttatcgt ttagtaacta
    18901 cattcttaca caaattaggt aagtatttac ctatcattta acgcgtttga aaacgtatac
    18961 gctgactgtg atccctgtgt cttcaaactc gttggtaaac gacttccctc tggcataaac
    19021 gtaattatcc atcgtcatcc agtccagtat tggcgcagtg cccggcaata ctgctacaag
    19081 acgcccgccg actttcagat gccccagcgc agccagcgtg tgctctctat gacgaccaag
    19141 cgagtacggc gggttcataa cgattttgtc gaactgataa cctacgttgt cctcagacca
    19201 ctttatgaag tcgcagcaaa tcgtgttcgt ataccctttt ccgcgcagga tatcagcgaa
    19261 gagaggtgcg atttctatgc aggtaacatc ttccagatcg gcgttgatat aggccagaag
    19321 atccccacgt ccggcttgag gctccagcag cttctcacca gacttcaatt cagtggctct
    19381 ggcaacgtac tcagcaatca agcgtggggt agggtagaac tggtgtgatt ttgtatccgg
    19441 tattaatccg gtagccacaa tcgtatttag cgtatggccg atctcatacg ggaactgcca
    19501 gtgctttttc tcctgcacgc cgccaatgaa gctcagtgtg cgctcaagct cttccacctg
    19561 cgacttctgg agagctgaat cagagaagta ccatacgcct ttgtctttgc tcaatcgccc
    19621 gtcacgaagc gcagtgcgaa ccggcacgga gatcgtcttc tggattaaac cgaactgctt
    19681 tggtgcccgt gtttttggcg cagttcggca tggcgcgggg attgcagcag gcatactgta
    19741 agccagcacc tcattcaact tccaggccac gtcaggatgt atttcaaagt gaacgttgcc
    19801 gttcttgaac atcttcacgc gcatcagatt tccatcgacg ttcatccagt caccggtctg
    19861 gcagtcgttt gccctatacg cagctgatag cacctcagca gtgcggttga tggtaataaa
    19921 ttctttgtgc gcgaagaaat gaagcaagac acgcagatcg tcgatgtagt cctctttgcg
    19981 atagttcacg ctaacgctgt cccgccagaa atcagaaatg cagtcagcga ttatcagacg
    20041 ctcgctgaaa ccgtttgtct tatttgtctt gtgcgcagga ctcagcgcct taaacaagcc
    20101 atacacgcgc tcagagagat atttgtgcct gtcattcagc aaattaagca tcgtgggtat
    20161 gaccgtttct gctttgaact ccggtacacc aacgaactct ttaaccttca tctggtagcc
    20221 ggttctgtca gttttgatga cttcctgttt gccctctata aactgctcgc gccactcatc
    20281 tcgacgggaa gctggcatga tcagcaaaac gttagtcatg tccgtgacct tcctccagta
    20341 ctcggcccaa atattctgtt tcacccattc cagatcgact ttatccaacc aacctcgatt
    20401 taaacgcgag cgatcttcat ctggtcgatg gtttagtctc agcaggcgat taatcatgtt
    20461 gtggcgttcg tcgccataaa cgaagtcgtg tacctgatac atgaaggcga tctctttctc
    20521 gcactcggcc acaatttcgt ggatgacatt catttcctga cgatagtcga tagccgtgtt
    20581 tgagctggca tcgtcgatga tagaaagggc tgtattcata attacaccat taaataattt
    20641 gttttctgat tggtgtaatt atcgcaaact tgaaaaggca taaaagctgc attcataggc
    20701 gttacagcag atttaaggcg taaaaatggg ggctttcgcc ccctgtcgac tggtgcagta
    20761 ttagagtgcg tattgcgttt tgtgcggctg cttattcaag tcatggcgat gcgtgagctc
    20821 ccgcatcatg tcttcaaggc gactctttgt gtcgtcgagc tggtcggcca ttgcacctaa
    20881 aagctggcga acggccatcg gatcgtcgct gtttagtgat ggcattttat aacctgcttt
    20941 agaagacata aggttaattg cagacatcaa catcgttaga gaggatttaa gcccggcaat
    21001 ttcacgatct ttgctggcaa taatcgtctc acttttatta ccgtcatgag atccttgagt
    21061 ctcgctcacc atatccaaag tcgcttgcaa cttatcagct ctctcttttt cagcaagata
    21121 atgaacaccg aaatgatgtg ctaaagctac aatctgagtt ggctcttcaa aggtggactc
    21181 aaaattaagt gctgtaacca ctctctcaaa tatggaaacg tcttctattc ctcgaagaat
    21241 ggctaacatt ttgaccagtt cattagcatc catttcttcg aataaggcgt tttgttcatc
    21301 agctatcgct ttgcatttct tgcacatgtg tttttcctaa tgcttaaaca agttgttttc
    21361 ttattggctt tattgtgatg tggcataaaa ggggagcaag ctaaacgtaa aggtgcaaac
    21421 tctctatttc ggtcaacgaa acacaagggc catttggccc ttgtcattaa cacgttgctt
    21481 tacggactag cggataagcg aagtagatcg ccagaccaat gaggacgcca tcagaaatga
    21541 cagacatcat ctttcccgta aaatccacca acaccgccat caccagaaga gcaatgacgg
    21601 tcacaagccg gaatctctca agcattagag ataagcatcc agtgacaact gaagcgcctg
    21661 tgcgatcttt ttcagggcca gctcttcttg ttcgccaata ccgtcttggt cggcaatatc
    21721 aaggcacaga cacagtacat ctactgcatc attagtcccg gccacatcag ccagctcacg
    21781 taaagcctgg gcattagcgc ggcgcggtga ggcttcgtat tgagcacgga tattggcgct
    21841 catttgggca atctcaccag caaacggaga aaacgcaggc agagctgcaa ttgttttctc
    21901 aagaatggaa atttcctttg cgtcgcatgt gccatcggca taggagatca tgtacgcgcc
    21961 ccacacggtg gcttcaaccg catctcggtt ctccattttt ttgacctcga taacagcttt
    22021 acgagttttc tttttgaata aacctaacat gtgctttcct tttgttattt gttaaaacaa
    22081 gttgttttct agttacatga attgcttacg aaaagacggt caatgcatat gcactaacaa
    22141 ccgtcagaaa taaccaagta ccgaaccgac tggaaaaaca aaaatcccaa caacacgagc
    22201 caaggtcata ccagcctgaa actggagatc accagagcaa acgagtttta caatgttcga
    22261 tacccaaccg gcggccatga gagctatgaa agccagaatc gccagccaag tttttccaaa
    22321 gtgatttgaa aaccagttca tacaaccgcc ttagtcacag catgatcctg agtaactgga
    22381 tgaacttgag gtatcgcatc caccgtcatc ccatccagag tgacaggacg ctctactgga
    22441 gtggtggtaa ttgtcgtcga cggaggtgtg gtgatggatg aacccggcat caaaaggctc
    22501 tggccggctg gaacggctac cgtcgccagt gaaactataa tgagaacgta ggccattggt
    22561 ttcagcctct ttctttgaaa attttccatt cgaactgtcc ttgttgtttt tctccttcac
    22621 taaaggccgt gtacttttcg cttcgcgtga agaaccagag gtaacaatcg cccccacgtt
    22681 cgcaacaact gacgcttgct tatctaaacg ttcattcagc aaccgaacgg cagtctctag
    22741 ttcatcaaga cgtgacagaa cacgaccgct aaataattcg gccaaaattt gacgtagtga
    22801 acgaggacgt ttagttgcag aagtgaaata ggtttgacgt gccatgtgga ctccatccag
    22861 tgtcagaaag agttgcggct ggcgattgcc agccgccttt ctcgttccat cctggaactg
    22921 tgtcttaccg acacattgtc atcctgacgc cgataagata catgatttaa aatgataggt
    22981 aaacacttac ttactgcttg ttgtaaacaa aaccaatatc tttcttgtga ggcatatctg
    23041 ctgcggaaaa agctgcgatc ttcgcgagtc gatcacatat ttcattttca cgatgcccgg
    23101 cgtgaccttt aacccacttc cagcgaacat tatgtcgact tgcagcctca tccagacgct
    23161 tccacagatc aacattcttt accggttttt tgtcagaagt cacccatcca ttgcgtttcc
    23221 accatttcat ccactgtgtc atgccgtttt tcagatactg gctatcagag tgcaaaataa
    23281 cgttgcatgg atatttcaaa cgctccagcc cgataagtgc ccccatcatc tccatgcggt
    23341 tattggtggt gctatgaaaa ccatctgaga actcgcgttc ctcaccacga tactggagaa
    23401 ctataccgta accgcccggg ccacccggat ttttaaggca agagccatca ctgaagattt
    23461 tcacggtttt aagctgggga ttgaactcta cgacaggcgt tgtgggattg gtgcggggag
    23521 aatttttgtt tttggctttt ttgcgagttt ttgcttgcga tcgggctggt gtcttcgtcg
    23581 tcatataaac tcctgaatca agcgccgcgc cgattttttt cctcgcgcgt gcgcacacgc
    23641 gtgcgtgtta ataattatta aaataaacaa attacttccc agaacaggtt tttataaacc
    23701 tgaactgaac gaacgaagtg agtgaagttc acctcgaacg aagtgagagg ttgtcttttc
    23761 aggtaatact ctcccaggga ggtgagtata aaaatccctc accaacctgg tcgtttcata
    23821 acctgaaaag ttatggtcta agtctactgc cagcttagac ttgggaagtt atggatgaca
    23881 gcaccccaga accgagatct tcccacactt tatgaagggg agtactggat tcaacctctc
    23941 gaaacacccc agactcgaca atcataaagt gacccttgtc tctgctcact ttggttcccc
    24001 cttccccgac acctatacgg caccggttct acgctggtag tgagcttttt taaacctgac
    24061 gccagtgacg cttaccccca cccatcaggt cgagcctcca gtctacgact ggaaactatc
    24121 agatcttagc acttacaatt cactttatga atagtttgta cttatctatc attgtgattg
    24181 attattttct cgaccatgta gttgaacatt caaggcaaac acctcattga tcaactcacc
    24241 taaaagctgt tcaacaagtt cgcgatgctc gccaagatgc aagcacttaa ttgcccactc
    24301 gtacaagttg aaagcctgct cacgatcttt catcatttca cgagcctggg ccagaaaatc
    24361 gctctctacg agcgcaacga cattagtcgg gtatgacatg ttggtttcct taaagtggtt
    24421 cataaaatcg attttaaagc gtctgggaag gggttctagt ggattctgta gtggtgttca
    24481 ggtctgggag tctctgatat aaaacagcct gcttccgtat attaataatt aatatgtagt
    24541 tatttatata tacagaagca ggctaattac agactccaga acaaactaag cagccttctt
    24601 tgatggtctt tttttcctga tcacattcgc aggatcatac cctcccagac ttttcatgac
    24661 gtccaaaggg atcttgctta tagagtgccc agcgtcctga caaaatcctc gaaaaacaac
    24721 gagcatactg ccaccggggt tgatgtttac ttctacctga cccaaattga cgtcaggctc
    24781 gacaaacgct acgcggccac cagacagaat aatcgtctca ttcgcacact ctgttgcacg
    24841 ctcataccac tttgtatcga gggattgagg aattagcatc actgttgtaa ctccgcgagc
    24901 ctgctcacgt atagccgcat caatccaggg ggagattttg gagtagggtg gattgaggaa
    24961 ggcaattgta cctggctgtc cccattcaga tttcaaagca tcacgctcaa cgcctatgaa
    25021 attaggcagc tttgcattgt ccttgttgca ggcaacgtca acatcaaatg tcactccaag
    25081 ataacgttga atggcagaaa acagccagtc aggtgttctc cagaggtcac gaagagagtt
    25141 gtcgcgcttt cgctgtttga ttttttcggc tgcaatcatc ttctttctcg ataggtaagt
    25201 gattacctat ttttgcattt gattttatgt ctggcaatca aataaaacgc gccagaaatg
    25261 caaacgagaa gctctctggc gcgtttttgt ggtgctgggt atgtgattgg cttacccagc
    25321 actataaagc tctctatggg ctttattttc agtaggggga cgtcatccaa tagtgtactc
    25381 cgctacaaac ttcttaacga tcggtgattc ttcgttcaat gttagatttc caccgtctct
    25441 aacggcaatt cctgtcgctg gaaatacagc catcatttgc ccagcctgag ttgacgcagt
    25501 gttaagcgga tacggttttt ctggattgct cattaaagca atcttaatac tattacttgt
    25561 agccttcttt tctgtgagaa ggtgtctcat ggaaattacc gtgtaaacac taatatctgg
    25621 acctccgcta aaccaattaa gaaggtttaa tatcttatcc tttgctttga caggcgcgtt
    25681 ttcgtaagcg tctaagaaaa tatctgtttc taagcctgct ttattgaaaa acgaatcatc
    25741 tttatcactt aaagtaaaac gaggacctct tgcagtacgc gcttttttat ttgtgttgct
    25801 atcatttgac tgttgaactt ttaattcgtc attaacctca acatactctt caacctcaat
    25861 aaatcccaat tgttctggct cgttttcggc agcttcaaca ctttcagcgt taattacgtt
    25921 tataggctca tgcgctaact ccaactcatt taaagcagag ctgaaatcac ctgctttttc
    25981 ttcattgaac agttcttcgg caacatcatc agaactcatg gctacgccag catcgaggcc
    26041 gagcagcaga tcgtcaatat catcagtatt ttcaacctgt gcggcttttt ttactggttc
    26101 atcaagtgat tgcaacatgg ctgtcagctc gtccagatca tcatttgtca ttacattttc
    26161 aaccgaactc atgtaagtct ccttctgctt ggttacattt gcgtttcgat ggctacattt
    26221 tgactaaacc tatcaggcgg aaaagtatta aatacaggca ggaaatgatt gaaaggcgac
    26281 tgttctttaa aagaaagcct gcttcaaact ctctttaaag caggcttttg ttttacacaa
    26341 tttcagaggg taatttaaaa aatccgtatt tattacgtgc cttaaagaag cattgcatca
    26401 ttaagtttgt gtcatataag gcactatgtg ctttggcatg atcgtatata aagccaagag
    26461 agaaggccaa ttcttcaagg cgaggacgtt taccgtcttc tgtggcccac agggaaaata
    26521 gagtgtcaat caatgggatg tctggaagcg aaaaaccgta acttttcagc tcatgcctaa
    26581 taaacggaat atcgaacgct tcgccgttgt gggctatcca aatatcggaa gaagagagat
    26641 acgaagcgac agatgaagcg tgatcggcta gtaacggctc ggcggctaaa tcttcaagac
    26701 tgatgccgtg taccgcttgc gctttcggat cgatactgcg acgcgggttg aaccgcatta
    26761 caaaactatc aaccattgtc tgtgtctcaa tttcgtactt caccattgcg atttcaatga
    26821 ttttatggcc tgagaggaaa tcaagacctg ttgattcgat gtccacccct gtagcaatca
    26881 ctttttatct ccttttactg gtcgtttgga cggtgtgaag atggcagctt caacatccca
    26941 ccccctcaaa attcgctgcg agataatggt cgcagtcaag ccaacctctc tagcccattc
    27001 ggtaattcgc tgagttctgc cgcgacactc gatcagacgc ttactatctc ggttggcata
    27061 cgtgtcgacg ctggcgttaa gcgcgcgatc taatggccac cctttcttga ttcgatagtg
    27121 aagagttgat gtctgaatcc cggctctttt agcccattgt gagagagtca gcttttcccc
    27181 cttatgctcc aaaattaagt ttgaacgcgt gttattggcc tgttcttcaa aagtcgccca
    27241 tctgcaattt tcaggttcgt agtcaccatt cacatctttg cgatcaagag acatcccctc
    27301 tggtcgctct cccatgtcag cataaaagtt ttcaaatatt tgccaccgct cgcagacttt
    27361 gatacctcgg ccgccatacc acttataggc gtgatggatt ggtttttcac atcgttcccg
    27421 catacccagc catatacgat aaattggcgt tttactcata ccatgaggct ttacgctacg
    27481 gcgatgtgcg ttgtagacac agccacacga tattgtcgcc ccgtgattta gcttcccgta
    27541 actaaccagg caggtgttgc cgcaatcgca gatgcaatcc caaagtcgcc cttgtttgtt
    27601 tgaaataccg gaatatttaa gtgcaaccaa ctttccaaaa cgtttacctg ttatgtctac
    27661 cggttgtccc attactactc cttacaactt cttagcgccc ttaagtagtg cgctacgtac
    27721 aaattgagcc gcttttttca aagtttcttc actggtttca cagacaaccg gatctttcca
    27781 cttacctgac gccgtgttga gaatgttgat ttggttgcta tccagacaaa cagagacgta
    27841 aagcaccgtg ccaccagcca gcatcaaatg cataggaaac agaggctttt tactgccttc
    27901 actgaactga gacatggcga tgttaatggc ttcacctact tgtgcgccaa caagcccctg
    27961 cgcagtttcg aatatggcac gaatagccag acgagcctct cgatcgctca taagagaccg
    28021 ggaatgttcg tccgctacac gaatcaaagc ctctgttgtt ttccgatcca gttcatcttc
    28081 aagcaacatt tcactaaaca ttttttatcc ttaacccatt ttgagagttt tattgtcgca
    28141 atgtcgaaga ggcgaacaat ttctaatgaa cggtcactcg gccacagaac cggttttcta
    28201 tcttttctac agcgttatct agcattctca tcactgatct ggctcttgct tcaaccgtgc
    28261 gatatcggtt ggaaacgacg aaggtttgca gatcccccct ttgcggatgc aggtgaattt
    28321 ttgtcagttc cccgcacatc aacgcatcaa tacgagcgac atacagacgg tctaatgatg
    28381 ctcgttgtga tcgcaagtaa tctcgctctg agagagcgac tcccgggcgt aagccagcaa
    28441 tggcgttaaa gtttgaaata gcagttttgt ggcaaaactt ttcgatatcg agggctaact
    28501 caatacatcg atcttcattg gtatggccga ccaaatccaa cgtgtaggcc ataacatcag
    28561 caggtgtgcg atcgataaca aagccttcaa caccacgcgt aagtagctct atatgctttg
    28621 ctatttccat ttgaatctga agccgctcat atagaggcat agactcacct actcgaacac
    28681 cgagtcggct catcaaactt ccgacaccag catccacgta agggattccg taatgtttgt
    28741 cgatatattt tgccagggtt gttttcccac tgccctgagc accagtgatc ccaatccggt
    28801 aatccattac gaccttctgt atacgatctg cgaaaagcca agctcttcct cgctggctcg
    28861 ttgggtaaaa actgtttcaa caggcacaaa gcctaaatta cgcatcatgc ttgccgggaa
    28921 gaatgcatcg gcgtttggca catcaacgcc gatgtgggag agccaaatct cttcaacatg
    28981 cggcatgaat agcgagtaaa tttgttcccc gccaatcacc cacacaggct ctggtaatcg
    29041 caacacatca tccacaccag cagggtagaa tccatttggt acaaagccat gagagcgcgt
    29101 caaaacgagg ttatgacgct ctggaagagg gcgtttgaga ctttccatcg ttttacgccc
    29161 cataacgaca gtggcgtttt tggtgagtct cttaaatagc ttcagatcgg tagaacaacg
    29221 ccagggcagt tcgttcgcta tgccgatctc atagttacgg ccaacagcag ctatcatctt
    29281 catttgctca cctcatagat gaccggtcta tgatgagagt cagctaaagc ggcacggagt
    29341 cgaggatcgt gcaccagcgc agcgataagc agatcgcctt tatgctccgc taatgtgcgc
    29401 ttaatatgct tctcataact gacgccattc ggtacgagat gaagccagtc gtaatcaaca
    29461 ccaaaatctt tcaaccatcg tttagtgggc gcttcaagag tttcagagcg gctactgata
    29521 agcaccactt cagcccctga gcgagcaaaa ccacgcaaca tacggctggt ggggaaaatg
    29581 agttcatcac cggcaatgag ctggcctgca tccgcgtcag atacagattt acgatggctg
    29641 gtatttgcca gcacaccttc aatttcgcat aagacataca tacctattgc catatcacac
    29701 cgccactgga accttgatcc acggtagagg gtcatagcca agaatcagaa tatcgtccca
    29761 tttgaaatca tccagctcag accattcatg agggaaaata acgaccggat ctgaatgctc
    29821 tggagcctgt cgtttcatta gctcacacac accttccatg tggttgttgt acaaatgcac
    29881 gtcaaagcca aaatgtacaa atgcaccggc catgtgaccc gtaatcttcg cgagaaaatg
    29941 agtgagaatg ccatagccag caatattgaa tggcatacca acaaaagtgt ctacgctacg
    30001 ctgtacgagg caggagttga ggatgcgttt ggggattcca agttcatcca gttcagtttc
    30061 agtaacactg ccgtccatct ccagatcgta tagcatcttg gtgtagatag actcataacc
    30121 gaggcgactg tgttgaagac caatgtcagt tgccatcgtt aaacgggttt caaaatcaag
    30181 ctcacgactc cacaaagaga agacaaagtg gcaaggtggc agtttcatat cctcaagctc
    30241 gcctacgttc catgcgttaa gcatgatgcg acgatcggtg gggttcgtgc gcagtgtatc
    30301 gacaatacgc tgtagctgat cgatttcacg ggacagcact acgcgatctt cactgatacc
    30361 caggtatccc tcgactttgt acccgcgttc acggaaagta tcgatcttgc tcagatattc
    30421 actatggctc acgatgcggg tatcttccca tcgacgccac tgcttaccgt aaaccggccc
    30481 caaatcgcca ttctcatcgg cccatgcatc ccaaatctta acgccgttat ccttgagaaa
    30541 ttggatgttg cccgtgcctt tcagatacca ctcaagttcg acaagcagtg gcttaagatt
    30601 taccgccttt ccagaaataa gcggcacagt cccaccagta agcatgtagt aagagggaac
    30661 gtagcaggtg cttaaagtac cagtgcctgt gcgatcatca gcctgcacgc cgcttttaag
    30721 caccgtctca ataacttttg catacgatgc gtttgttacc tgaccgttcg tatgctctcg
    30781 attcaaaagg agtgacacaa aaacctcaca aagataagta attacttatt catcgtgtga
    30841 gtttacattc attgagagaa caagaccaga cagataaaaa aatggcggcc ataggccacc
    30901 aaaacgaata gtcattccag agttttatta cgcatgttaa atatacacag atagataact
    30961 acctacaatt attttttgat cattccgtac attgctgagg ctttagcttg ctcaacaaag
    31021 gaggataaat cgacatcact ataggttgga gacttcataa tctttccatc ggaaattcga
    31081 aaaccgatca tcttatcggt gccatcagca tggcggaatc ccagatctga agagtcgtat
    31141 ttgcagttgg ccacagcctg gcgacgttcc tcaacatcag caggccataa ttttgtcatg
    31201 ttagaccgat gaatttcgcc aaccagctcg acgacattga taccgaggaa atcagccagt
    31261 cgataagtca tcatgcacgc cacatagatt ttgttcatta cacggcgcag ttcctgaatc
    31321 agctcagaat ccttaacagt cttcttctca agtttatctg ccagctcttc gagcatacat
    31381 gacgcctcac gagcttcctg aaatggcatt gccatatcat cgaaaacagt gttaccgggc
    31441 acaaaaattg tctgcataaa gcgatcaatg ctctgttcct gcgtgtaata ggtcatgccg
    31501 gtagaaatac cacctttgat ggcgaccatc gtaccaacac caacgtataa aaagtcggcc
    31561 attgcatcca gcagttgctc aatatcccca ttcattgcgg ctggaatacc ttccactaca
    31621 gcttcttcat gaatcaggct tgcacggaga cgaagcagct ctggtgtcgg cataacgcgt
    31681 ctcggatgct ggaagagttc atgaaactga tcgaccatct tgtaaatact ttcagtagct
    31741 acaccatgac cttcatgcaa ctcatatggt tctggcttaa atccaatgag acgatcggtg
    31801 gcaagtttca ggtggtcagt cagtttggta aagttcatgc tagttttgtt ccttcttaat
    31861 tcttgtgatc cattgtggcc cagcgttgcc aggccacata atgctttgta tcggtttaca
    31921 ggtctgcaaa ctggcttaga gaagccttat ccacggtaga atcgatctga cctaccagat
    31981 atgtgctttg ctctgcctct tgtggtgcaa tttgcagtgt gtcggttaac aaccacttgt
    32041 tcatccagac cagcggatct gtggtaactt ctggaaataa agccttcagg ccaagacgat
    32101 tcatagccag attggtgcgg tattttacat aggtcttcag aatatccgcg tttaaaccaa
    32161 tcatcgaacc gtctttgaag agatagtctg cccagttcat ttcttgttcg gcgactgatt
    32221 tcatggtgtc gtaaatgacg ttttcttcat cagcagcaat ctctttccac aataagcctt
    32281 cgcgaccggt acgcatgaag cggatcatgc gttcagttcc ttcgcaatgc agagcttcat
    32341 cacgggcaat aaagcgcata attttggtgt taccctcaag caacttccgt tcgccaaagg
    32401 caaacgtaca tgcaaaactt acatagaaac ggatcgcctc aagcgcatta atggacacca
    32461 acgtgcggaa aagctgacgg tgcaaagggt atggctccca gccatattca tttacgtaca
    32521 gacgttcaaa ctctttctca cctaacgact ggcgctcgca ggtcatccca taaagcctgt
    32581 catattcagc agagatactg atcgctcggt tgatgatttc ttcatccgta acaataccgt
    32641 caaaaacaat actcggatcg tccaccatgc cacgaataat gtgggtgtag ctgcggctat
    32701 ggatagtctc agagaaagac catgtttcaa cccacgtttc cagttcagga atagagataa
    32761 gcggcagaag cgttgcattt gggctacgtc cctgaactga atcgagtaac gtttgataac
    32821 ggagattgct cagaaaaatg tgacgttcgt ggtcccgcag cttcgtgttg aagtcgatac
    32881 gatcactcga aatatcaact tcttccggcc gccagaagaa agacaattga cgttcaatca
    32941 acttttcaaa atcacggtat ttttgctgat cgtaacgtgc cacgttgaca gattgtccga
    33001 ggaacatagg ctctttggtt gcatcattag cacccaaacg gaacgttgaa tatgacatgt
    33061 gtttttcctt ttttgatgtg tttaaacaag ttgttatctt atgtgtttaa acaaggctta
    33121 caaatcaaag ataaggtggg gatatacccc accatatgtc agattttgca ggctccgtcg
    33181 cactcatctt ctggctcgac ggctacagct tgcgtaagtg gattctgcgc ttcgtcgtca
    33241 tcacgctttc ctgcaccatc tctggtgttg tggtagtaaa gcgttttaac tcccatcttg
    33301 taggcgagaa gcaaatctga cagtagcgtc atcattggga ctttatcgcc ttcaaagcga
    33361 gaagggtcat agttggtatt ggctgaaata gcctggtcaa agaacttctg gatgatcgcc
    33421 acctttgtca gatagccgcg gttgtccggc atatcccaca ggtattcata ctgttccttc
    33481 agtttttcaa actcaggcac gaccatctta acgatgccgt ccttagaaga tttcaccgac
    33541 accgggccac gaggcggttc gatgccgttg gtggaattag tgatctggct ggaagtctcg
    33601 catggcattt gtgccgtcag tgtggagttg cgaaggccgt attttgcaat acgttcacgc
    33661 agttcttccc acggcatctt taactcaaag ttggtttcgc cactttcgtc taatgaagaa
    33721 cggtaatggt cgatcggcaa ctgaccaatt gcgtacttgg tctgctcaaa ccagtcacac
    33781 tcaccttttg cttcagcaag tcggcagctg gcatcaagaa ggtaatactg gatagcttcg
    33841 aacgtttcat gcaccagttt attgccagcg gtatcagaat aacgaacgcc attctttgcc
    33901 aggtaataag caaagttggt tacaccaatt cccaagctac gacgagcttt ggcaggcact
    33961 tcggctgcct ccatcggata gtcttgataa tccagtaacg aatccagtgc agcaacggca
    34021 tagaacgcca cctctttaag ggactccagt gaacggatgg ctccgaggtt aaaagcggat
    34081 aaagtgcata gcgcgatctc accgttcggg tcgtcggtaa atgccagtgg acgagtgggt
    34141 agagtgatct ccatgcacag gtttgactgg cgaacaggtg caagagcagg aatgaaagcg
    34201 ccatgctcgt taatatgatc gacgttcgca atataaattc ggcccgtgga cgctcgttcc
    34261 tgcatcagag atgagaacag ctcaatggca ggtactgact tcttgcgaat tgaatcatcg
    34321 gcttcgtatt tatggtacag cttttcaaac agatcctggt ctgtgaagaa ggcttcgtac
    34381 atatcaggca catcatgcgg gctgaacaga gtgatgttct cgcttctgac gagtcgacgg
    34441 tacattagac ggttactcat gacgccgtaa tcaagatggc gaacgcggtt ttcatcaata
    34501 ccacggttat ttttcaccac cagcagactt tcaacttcaa gatgccagat cgggtaaaac
    34561 gctgttgctg cgccaccacg aacaccacct tgcgaacaag atttaacagc cgtctggaaa
    34621 tgcttcagga atggaataac tccggtatgg gtggcttcac cattgcggat ctcgctgccc
    34681 agcgcacgga tacggccaaa accaatacca attccagcac gacgtgaaac gtagtcgata
    34741 attgcggctg aagctgcact aatccctttc agactatcgc cagactcaat cagcacacaa
    34801 ctggagaact gacgggttgg agtacggacg ccggccatga ttggtgtggg cagagacagc
    34861 ttgaacgtac ttgtaacgtc atagaaacct ttgaccatct ccatacgtgt tttgcccgcg
    34921 cagtcgtctt cccaattctg gaacagacac atgcccacca gcatatagat atgttgcggg
    34981 ccttcgtgaa tctcaccagt aacacggttt tgaacgaggt atttgctttg caactgcacc
    35041 gtagctgcat agccaaaaag atcgtcacgt ttaggcttaa tgtaattgcc gagttcttcg
    35101 atctcttcaa atgaataatg tgtcagcaaa tccttgtcat aaacgccctt gttaacgttc
    35161 ttgacaatgt gttggtagaa gttcggatag tcgtaacgac cgaaggcatc tttgcggatt
    35221 ttaaaaatgt tcaggcgggc agccacctgc gagtaatttg gtgcatctgg tgagatcaaa
    35281 tcagcggcag atttaaccaa agcctcatgc aactggctgg tggtaatccc atcaaaaatg
    35341 ctggtcgcag ctcccattgc tacagcggat gcgcttacac cacgaatgcc ttctacgcca
    35401 tacattacga cgcggttgta tttttcttcg gataacggct ctgtttggcc gttacgtttt
    35461 acgatgctta tcatgtatca ccaaataaag agggccacta tatttagtgg cccatgttaa
    35521 tagataagca cttacctatc aatgagatca aatgagagac ttgagaacat cgcgcacctg
    35581 gcgaaactga tctgtttgca taccggtatg gatggcagcc accgcatcag ccaaatgttc
    35641 gtttttgttc acgagaacat cctgtccaga ttgcttacga cgcagccacg gtgctttagg
    35701 gtgttttttc gtagcccact ggataatctc ttctttcgat gtagtaagtt tattgccaac
    35761 aaaatgcttg atttcgtttg gagtgacctg gatcaatggc ttatccacac acgcaagtac
    35821 accgatacaa ataccgtaag acgtctgcga acgagagttt tggctcccaa ccggtagttc
    35881 acaaaaaacc atattcgcct tatcaatgat tggcttcgca acacgccata tttcactggc
    35941 acggcgcaga tcgtcactgt tcacacgaac ggtctttttg ttactccccg ctttagtctc
    36001 aacaagagta aggccgtgaa tctcaagttc gtccgtttca aggtctaaag tgcccacagc
    36061 aagcccaaag ttgctcatag agggatcaac accaactacg ctgatttttt tactcatata
    36121 gtctccttac catgttcccc atagagggtt ttccaataat ctcgcctcca acgaattggc
    36181 tactattgcc gtttgcaacg ttggttgggg atagtttgca aagtctgaat actcattttc
    36241 ctgcgtgaca cgtttatcta cggcatgttt aacgccactt gctatgaaat gacgcatgat
    36301 gcccaacagc ctccgactgt ctgcactatc gtttgctaaa acatgaggga gctgaagttc
    36361 gatttctacc atttctccat acttaccaac aaactgtagt ttgtccgcac tgtacttttt
    36421 atccctcctt tggataagga aaccagtaaa agcatcacct ggcgatgcca aactatccac
    36481 ccgcttattg gcaagtcgaa taaggttcgt gaaagccata cagagatgcg catccattgc
    36541 cccaaagaac ccttcgctat caatgttaag atcttcagtt tcatgggttg cgataaaccc
    36601 aggcccgacg ttgggaagat atcccccctc aacaaaagga ttaatcagga tcggcgcaat
    36661 acatccaggt ggtagtgatt tcaaactttg catcaatggc gtctcggata ttagaccaag
    36721 caacgcggcg aattgtactc cgtcaatatt gttgctgccc cacagaatac gtggagccgc
    36781 accattggaa gtatgcccat tgatcagttt tatcgactgc atcactgcaa atacctgcaa
    36841 aaactgcgag cggcttaatt taaacgacat agctgcgccc ctctttaact tccagcgtaa
    36901 tagtttcccg gaaccacgac ttcatctctt tatgggagat aatcagcaca gtacctcgct
    36961 cgcgagcttt ggactccaga atacccatga ggcgttcaag accggctgta tcgagtgcat
    37021 cgtcaatttc gtcgccgata aacaaatcga tgtttttact cgcccggtta ctaaccagat
    37081 cctgcaatgc caaagaacac gcaatgcgaa ccttccgctt ctcaccacca gagagggttt
    37141 ggaacgactt acttgaacct ttcttctgca cactaatgtt gaatttgtcg cgatactcac
    37201 cttttttagt gacatccatc gtcgaccact cagcagtaat attcccgtca gacaacgtat
    37261 tgagatactc ggcagtgcgt gtattcagga aaggcgtaac agacgtcaaa atatgagaac
    37321 gcaccccggc aggagagtag acctgacgag ctttttccaa tagcaacgtt tgttcctgaa
    37381 tagccttcaa ctcatctttt aaggacttga aggtagactt actggatacc aggttatctt
    37441 cgtgtctggc gataagagca ataaacggat taatctcttt tgatatacga tcgacctcag
    37501 tacgagcacg agtcacaaga gactcaatag caacaacctc cttctcacga tgacgcaaag
    37561 atgagagttg tttcgtaagc tcttcaatcc gggcaatgat agccgttaca tcaggtgtcg
    37621 ttttaacaag ggcagactca atgcttaacg ctttctcaag attcgttttg tgtttagcca
    37681 ttgcctctgc aagtgtcttc gcctgaccaa tttcctgacg tgcttgttca atgaaattct
    37741 ccttcaccgt tgatagatct tcttcgcagt aggctttgcc acaagtaggg catggtgatc
    37801 ccactttagt accaacctct tctgctttgg tcttaaaatt acgcgcgcgg ttcattgcat
    37861 cagcctgact attttctgta gctttgatgc ttgcccgaat atcagttatc gcaccacgaa
    37921 ctttgagcaa cttggcgtca tgttcttctt ttgaggctaa ctttttgcgc tcactttcga
    37981 ttgccttctc ggtatcacgt atctgttcgg gaagagtgcg gatctcaagt tcaacttccg
    38041 ttaactcaac ttcggcactg gccagcccag ccagggcatc gtcataacgt ttagaacgct
    38101 cttgctccca tgattcagag gaggctttcg ctgactcaat ttctgactgt gttgcctcaa
    38161 tggtcgagat tgtcgactcc aatttggttt taaccacatc catacgtgcg gcagctgcat
    38221 tagctcgctc acgagcaata gcgtaggcgc gtgtcagtct gtcaacgcca gcggcttctt
    38281 ctacgatggt tttgaggttt ttgtcggaca ttccaggtaa atctggcata gcttcttggc
    38341 tcgcatagat ggaagccatg aaaacctctt tagatgcacc gatcagacgc tcgacgaact
    38401 cctgcgtcag cgcatctttg cctttcgtca tatcgccatc ttcaccacga acgataagac
    38461 gatttttgaa ctctttgtgt ttacggtgac gaatgattgc gtatctcttg ccttcatcct
    38521 cgatggttac tgcaacacga cagttctttt catggtcggt agacaacaca tcgtcaccct
    38581 tcacaccatg agctgtttcg ccataaagac accacatcag gctattcatt agagttgact
    38641 ttccagagcc attacttgat gcggaactat catcactatt aacaccctga atgagcacta
    38701 aaccacgctg atctaactcg acctccgcgc tggcgatagc cataaaattc tcaacctgga
    38761 gctttaaaaa cttcatataa cacctctgat accgtgtgtt agcttttggc cttctttcaa
    38821 tctgaactcc gtatgcgccg ggaaggatct gcgtctcaat acgccttttc ttgttaacct
    38881 tccaagataa aacgctacgg gatttccttg tggcgggtag ggcttgtgaa acatcaccgt
    38941 ctggcctcgt tcaagtcttt tcatcgtgat ggcaaagtct cgaaagctcc cataacgact
    39001 ttgcttcaca cagcctccga actctccgct tcggtgagga tttcctgaca caaaatatcc
    39061 aatttgctca gatcaaatcc gccgtcagta tcgtggacaa tcttgcaata agcagatacc
    39121 gactctccca ggctatcgat tttgctggtt tcagttgtgc tggctgtccc ttccatcatt
    39181 gatgacttac ggatgaagtt gcacacgaca ccttttgcac ccattgtttt gaggatgttc
    39241 tggtacttaa tgccttcttc atcgttctca attacggcgc ggaaacgcac gtagttgccg
    39301 cgaatttggt catcagcaac atcatcttcc aggttaatga atttaggcgc actggtttcg
    39361 tagtgactga aactgccgtc cgggtttacg atcatgtagc cagccagaga tccaacatct
    39421 ccccaatttt gatgagtcag tgcaccgaca ctgataactc caggaatgac ctctttgtgg
    39481 ttgtggtaat gcccactaag cacgagacga aagccgatat cctttaactc ctgcgcatcg
    39541 ataccgacgt caggcattgt tggaatggct ttgttaatgg atgtatggat aacaacgtca
    39601 tgattgtcac cttctacgct cttacgtaat gctttcagat cgctgataag ctccgcatga
    39661 ttgttacgcc agctaatcag gtggacagtc acatcaccaa tttttattga gtgtgggcac
    39721 ttgccacata cgattaccac gccgatagaa ctcagcgatg ctgctgcgtt ggcgctatat
    39781 actgaatcgt tggtttcaag atcgtgatta ccggccagca ttactactgt cagatcaagc
    39841 tcgttgataa tccacttgta cgtttcagtt acgtaatgca aaacagaagg ggacacagtt
    39901 cctcggacgt gaaatgtatc accggcaacc aacatgtacg tacaaccggc cttcttcatg
    39961 gcgatggctg cttctttcgt tgcttccaac tgtatttcaa gtctggagtt aagcccctca
    40021 gcgttcgtcg tggagaacgc atcccatttg tggtagtggc agtcagaaat gacgccgtat
    40081 ggcaaagtca tgtgtttttc ctttgtggtt attttgatac aaattctaag agcaatgagg
    40141 aggtaaacaa ctatgcataa cggtatgtta ggaatgaaac gagcgcctta atatacaaaa
    40201 tttataagta agtatatacc tatttatcgt ggcgcaactt tgcttgtctg tatgactaaa
    40261 aataagactg gatcactgta gaacgctatg agagctattt tgagggggcg tagattctat
    40321 ctacgccaat atacgatctg gagggttaca gcgcgtcagt gggcttttta cgcgctgcat
    40381 ttatgcttat gacgccagct ttaggacgtg gatcgacaag gttcagttct tcttcgctat
    40441 ggtattcaag gtcgaactca cgttttacat gtctgatgta gattgcagtg agcaggctat
    40501 ctttggtcag gaagtgtccg taggatttgc ggatcacttc ttcaaccttc tctattttct
    40561 caccacccat gcagaggtga ttaaaccggc tgtgtttccg caacatctcg tcaactgggc
    40621 ctgagtaaac cttgtctact ttcccaaaac gaatgatttt tcctgtatct gcggatacaa
    40681 ggcaaatcag tttgtctggt gataaacgat ctcgccacgt tactcctgaa cggagtgtgt
    40741 tgaagtaggg cgcatccaat ccgatgatcg gtttacgaaa tgcgagcaat ggaacgtacc
    40801 tgatacaact attcaaatga aagttcacac cagccttatc aagtttaatt ctggtttcgt
    40861 agatcgggca cttcgccgct ataccgcaga tatcacaaag taacttctgc ttattcaggt
    40921 tggaatttga ttcgatagtg taggagccgt cctcaagacg acgaacccag cgcgtgcgtt
    40981 ttagatccat aatcgttttt tcagtagtta ttggccgatg ctacgataac tcattaggca
    41041 taccttaatc tgcatttgcc tgttttactt atcccagtta tccactggat agatccaaat
    41101 aagagatccc aagtaagatc aaatagaaga tccaaagaga tccccgatcg cctctagccg
    41161 ttgcggttac tggtctgaaa gggtgttgcc atccactatg gagagcaaag aaacctctgt
    41221 agagagctga ataaccgcta tgaagagtaa aacaaccact ctggagagct gtatatatcc
    41281 actgtagaga gtagagcaca ttgtctttta tggataactt aatgacttaa ttcttcatta
    41341 acatactgat atacatagat atattttaaa ctctactctt agtcatgatt cttatgtcct
    41401 taaatatcca cagaagagta gagttacctg ttcttgaaat cctctgattt ttatccacag
    41461 gcccgatcca gtaattcaga tcacaaagag aaccaaagag acccataaag acccaataag
    41521 atccccgccc acctcaaacc gttgcgccat ctgccttcag acatgatcga tatccactat
    41581 agagagcgaa taatccacta tagggagcaa agcaaccact atagagagga gaaaaaccac
    41641 tatagacggt aggataaccg ctgtgatgag tgctactatc cactgtaatc agcaaaggcc
    41701 accaaagccg tcgaataaca agagacattt ggaatgagcg aaaacggcaa taaaaacata
    41761 gcgatcgttg aagcattttc agaaacagat aaaaagacag gcgaagttgt tacgttggtt
    41821 cctaacacta ataacactgt tcagcctgtt gctttgatgc gtctgggcct cttcgttccg
    41881 acgctgaaat cgacatccag aggaagaaag gggcaaatgg tctctatgga tgcctctgct
    41941 gaactcaaac agctgtctct ggcaaaagcc gaagggtacg aagacataag gatctctggc
    42001 cttcggcttg atatggataa cgactttaaa acgtgggtgg gaataatcca cgcgtttgcc
    42061 aaacacaagg tcgttggcga tactgtcacg ttgccgttcg ttgagtttgt aaggctgtgt
    42121 ggcattccga ccgccaggtc atcagcgaag ttacgcaaac gcctggacag ttcgctaagc
    42181 cgtattgcca caaataccat ctcgttccgt agcaaaggct ctgatgagtt ttacgtaacg
    42241 catcttgtgc aaacggcaaa atatagtgtc aagcacgata cggtcgaact gaaagcagat
    42301 cccaagattt tcgaactcta ccagttcgac aaaaaggttc ttctgcaact ccgggctata
    42361 aacgaactct caagaaaaga atccgctcag gcgctttata cttttattga aagtttgccg
    42421 cctgatccgg caccaatatc actagcccgt ttacgcgcca gacttaatct gacaagtcgc
    42481 actattactc agaatgcgac ggtaagaaag gctatggaac agctgcgaga gataggatat
    42541 ctcgattaca cggaagttaa gcgcggaaac tcggtctact tcgtcatcca ctataggcgg
    42601 ccaaagttac gccaggcaca aatatcaaca aaaatcgata atgatgagac ggaatatagc
    42661 ctcccagatg agaatcaaga tgacattatc gacgttgtgc ctgacgaaaa agaaggcaaa
    42721 atggtcatgt tgagtaaaga agaactggcg cttctcgaag agttgcgcaa ggcaaagact
    42781 cgcaaatagc ccaaccactg tagttagtag taaggctcac tatccactgt agtgagcctt
    42841 acttatatct gtaattcgct atccactata gtgagtatcg ttatctgttg ataaccactg
    42901 taggcagtag aatgcaaata ctgaatcaaa tgttagtgat cactaacact gatgaaacaa
    42961 gggctgcgag gttagtggtc actgggtatc ttggaaaatt tactgtctat agtggttgtt
    43021 gtcgccctca tctttgagcg tatccactgt ggcgagtgga tatccactga aaacagcaaa
    43081 ggcttactca ccatagtgga tacccgctgt acatctcact ccaatactca ccatagtggt
    43141 tgtttgatgc gaatgaaagg ctgtctactc gctacagtgg ttagcagata tgtaggaaga
    43201 gaggactgca gaccacagtg gataatgagc tgatacgaat gcggtgagct ggctactcac
    43261 cgcagtggtt ggtgctatta gtgtattttc ttcaacaggc cccaaagtgt tccggctttt
    43321 gtagtcagtt ttcctgtttc tggatcgtac atacgccatt cacgccgctg gtggacgata
    43381 tacccatctt cacgttccag acgctcaaga atgccaggct gcttgaaacc cttagcacgc
    43441 cagtagccgc ttgttttctc gatttccaga cctgccagtg ttaatgccat tatccaacct
    43501 ccttgcagtc atcgaaaagg tagctttgct gagaagcgtg aacgccatag aagcccttgt
    43561 atttgttgta tacgaagtgg tcttctccga cgccggttaa tttgccatta cggtttgtga
    43621 gatatgggga tgacaacact cgatcgtcac gcacaacata gaactggtcg ccgctgtcta
    43681 cgaccatcgc tccatatggt gcttttatga cgtcggtaag agcgccattc ttaacgtcgg
    43741 ctacagacat ttcacactga taaacagtgg tgtcggccac aacgatcgag gaaaggaaca
    43801 acagagtgaa tgggatggcc ttcatgctgc cacctctttg attacatcaa atgctgaatt
    43861 aaatacgagc acgatagcca aagcgtttat gactacgtcg ttcaccgggg aaaagacttt
    43921 acgaatgaca ccggtgaaaa tgcagtcgat gacaaaagct attgaaacga tgagtaacaa
    43981 gtattcaata aaaatcttca tgataggtag atactaactt atataaattt tactgtaaac
    44041 tcactcaaaa gtgctgatgc ttaaaatacg ctcaagatgt gcggcatctt catcactgat
    44101 ttgttgagtc tcttctacgt accaacagtt gccgttatgc caacaaacac catcatcatc
    44161 aaccatcact gaatcagcgt catgacctgt atattcaaca agagtacgct ggatcagcgc
    44221 gtcagtttct tcgtcgttaa ggccatcggc tttgacgata aaaagcggaa atgcgtcata
    44281 gacagtgctg gtaacgattc gaactaaaac tctcatgtgt ttttcctttt gtggttagtg
    44341 tttacttatt ttggtagtta tgaataggcg atcaatcttc ttgtttaggg aaaaactgtt
    44401 caataactct gtcaacggct gtgtctatat ggagtagatg ccacacaacc atatctttgc
    44461 tccccatgcc ttgtgtgaat atcacgtcat acttacgcca tgcgcagtgg aaccaatatg
    44521 ctgagtcatc aattggtaaa tcctcccccg cctctttgaa gtatttcagc agcattgctt
    44581 gctctgccgt tggcgttgga cctatttcct gagcgtacat acggatgaat cgctgccagt
    44641 ctgtctcgtc gggggttgga tctggtgggg tcatttctgc gatagcgttt cgtagctgat
    44701 gcgcccaatc aggttcagga aagccttttg ctacttcatc atcaataaca tccagagcca
    44761 cgtttgcggc atcgtagagt ttcttaatag ctgtcatgtt ttcttatcct tttaaacaaa
    44821 ttgttttctt aataagataa ttgcgataaa aaaggcgtcc aactggacgc ctgaactttt
    44881 taggggagtt atttcactgt aatgaaagga gtgttagccc cttgagtcat gtactgcggt
    44941 aactggccat tccatttatt gatggcttcc agttccataa cgttcgggtt ttgacgcaga
    45001 gcttcaccgc gcagacgaat ggcgtctgct tctgcttgcg cacgagcacg gatagcatca
    45061 gcttcaccat tagcctgttc acgtagcata ttcgcctcag ctttacgctg ttcaacttcc
    45121 tgctgacgtt gcagagtacg ctggttagcc gttactttgg cgttaataga ttcaatgacg
    45181 gtttttgggt aatcaggctt tccaacccat gacaggctca gtacctcaat accaaccgga
    45241 gacatctctt tctgaatatc tttcagtgcg ttgtccagca attgtgcctt accaccgtcg
    45301 ataaatgagt cagtggtcat acggctggcc aaacggttta gagagtcggc aattttctga
    45361 cgcagatctg attcggtgat atcgtctacg cctttgcgat aggtctggaa caccgtcgtt
    45421 accttgtcac gattaaccag atacgcaacg ccaattttgt gaccaatagc agtaccgtca
    45481 ctcatctgga atgtgaacgg ttcgtcgtag gtcttcattt gtttgaaggt cgggaatacg
    45541 taaagttcgg tgttaagacc tgtccattgg cgaccaacgc caaccacttc accaatccct
    45601 ttgtcttcac ctaatttgtt tactttgatg ccaacgtatc caggctccac gcgatcacag
    45661 ccagtaagac ccattgtgca aatagccgcc agagcaactg caagtaaacc tttcttcatt
    45721 acttatttcc cctttgcttt gattaatgag ttaataaact tacgaccacc aataagaccg
    45781 attactggag cgccaaaacc aacgagaact ccaagcatga cggccaaatc actctttgca
    45841 gaaatcagcg acggaaccag taatccgtag atgagagcca ctgaaaggcc tgtggctact
    45901 gccaatagat agattttgat catgtgtttt cctatgtggt tgttatgtga aaaatgataa
    45961 gtaagcactt atatattttc aagagataaa aaaggcgtcc tgtggacgcc ttgtgtttac
    46021 tcttctactg actcggtggg attgtctttt gatctccggt cgtcgattgc ctgtaaagcc
    46081 gcaatgattt cagctagtgg cttctcccga tacatctcga cgatctgtga tttggtgtat
    46141 ttcttgtcac caatttctac tcgaccgctg gcgttctttg gcaggtatcc ttcttccagc
    46201 atgtactcga cgagcgactc gataacgtcg aggccacgag taggatcgaa gtagaatttc
    46261 caggtgcatt tgccatacgg tggtgcaact ttgtttttga tgcattctgc gcctacgtcc
    46321 tggccgatct tctctttacc atccttcatc accgatgcac caagacggat acgtacagaa
    46381 gcgtagaact tcggtgaatc tccacctggc gacgtagtag ggtcgccaaa cattacaccg
    46441 atttttgtgc gaacctgatt caagaagatg atgcaggcgt tgtatttgcg tgcccacaaa
    46501 gccagagtag ggaagttcgc actcgtcgca cgagccagtg ccgtattgtc gttcatgttt
    46561 agctgatctt tgtctttggc tgtgccttca gccatctttt cgaacttctc ggctttggag
    46621 ttcggaacca tagacgcaag agagtcagcc acgatacaga taggtgctga ttcggggata
    46681 agctcttcat cacgaaccaa tttaagtatt gtgccgatca actctacaga gtcttcgaag
    46741 gtatctggct gcttgtatac ccactgaccg tcatcctcat ctgcgttcag gccattggct
    46801 acagccaggc caacgtcaaa actgttttca tgatcgagga ataccgccag accatcttgt
    46861 ttttgagcgg agatcatcgc tgctgttgca aggaatgtgt tgtggctgat aatcccgtta
    46921 gaccagaaag agtgtgtttc cggcatcgct acgtcgaacg ttggtgcttc accttcctca
    46981 atagccacga cttcgtcgta gaacaagtta gagccaatta ccgctgccag ttgtgcaaac
    47041 agatgctcgt taaaacgatt ctttctgcca atgaaggaga catagatttc tcgcagtttg
    47101 tctattccga cacgatcgcc gcggccaata acgtggtcta ccagagcgta gtcttcacgg
    47161 gtggtcttgg tgagcgactc gtagagtgat ttcactaagc cgctgatgtg cggaacatag
    47221 cctgagtaag tgcggtcaaa accaatgtcc cgtttggttg ccactgataa acgtgccgga
    47281 gattcgaaac caatcgttga cagaaaaagg tcgtaattag agccactgaa tgacagccgg
    47341 taatatgtat tttcataacc cgcgacgtgt ttttcagaga tagttgacgt aatccccaga
    47401 ttcaggagca tgaggcgaat ttgctgtagc agcagcccac tcgcgctcac aacctcaatg
    47461 cagcgaccat cattgacgtg gcattccagc tcaaagtagc cgcgaaggaa tgcaatttga
    47521 gcctcgctat tggcgcgacg cacactcaac ggaacctgct tgccagcagc tttctcatac
    47581 tccagaccat attctttaaa aagcagcgaa cgcacctctt tgctaaacag cacatggtct
    47641 tccgagccgt tatgttttct cgtaactggc atcttgtctg ataccagcga aatgaggcgg
    47701 tagtactcat ccttgatgaa aggatctgtg ttagaaaaat tcactgaatt ttcagaggcc
    47761 acgtatccgt cagcaattaa atagcccatc agttttgcga tattggcatc caggtgctga
    47821 tcgccgaatt gatgtgtgcc aaccattgaa ggaatcgtgt cgcctacact gattttttca
    47881 gcatgtcgcc agacgacatt acctaagtcg tcaaccacac ggattgggtg acggaacgta
    47941 gcctcgatat aaccgcctga tgccagctta atacgcttga atttgcgacg gttgttccat
    48001 gtcaggtgtg aggtcttctc tatcacgcca ttttcattga tgagtccaac gttatgctct
    48061 acatcgcgag tagtgcatgt cgctttgtac ccttcaatct caaacaactc tttcactgtt
    48121 accattccac gctccgtcag cagcatggtg tctgcggtaa cacatttccc cgcgcttggt
    48181 ggcccgaata cttctacgat acgaccacat gggaaaccgc catcgtaacg accggaaata
    48241 gctttgttta acggaggaaa gcctgtgtca atccaatgag ttactttctg aatttcgtcg
    48301 ttgctaccga ttttcttttt cagagcaagt gccagtgcgg attttccttt tgccatgatc
    48361 aggctccttt tgtttcgttg attcgtttta aagcggcgga ttcgtcgaat acaatcgcat
    48421 cgtggttaag gagtctggat acacgagcga ggatttttac gacctgctcg ctgactaatc
    48481 caaactcgcg atctgtcgct ttcattccgg cagcgcctag aattgacggc agtgcgatga
    48541 cagcgtactc accgtgataa aagacaatct cttttgccag ttgggtgggg gtggttgtag
    48601 cgccattaat aatcgattta agcattagca atacctttcg aatggaagaa caaacacttc
    48661 aaggtcttca aggaacgaac ggaaattcag ctcgtagcac agttgctcga atgctttcac
    48721 gtcacgattg cctttgattg tttcgatttc ggtaggcgga aacttcgtct caatcaggtt
    48781 cattagcgtg atgtttctct tgaacgcttc gagcattcga cagcctgttt tctcgttgaa
    48841 ggcattcttc gctagtttgt tgaaggcggt cttatggcgt cctttgtcaa ccacgattga
    48901 gccgtcgttg atgccgcgta ccatcgttgc gacacttccc cattcatgca gcagctcttt
    48961 cgcaccgcca gcaccaatgc caccaacacc gctaatgttg tccgatttat cgccttgtaa
    49021 tgcttttgct tccaaaaatg cgcgaggagt agcgaatcct gtcagctccg caaattgctc
    49081 aaaattaacc tgcttgtttt tggcgtcttc acgcaggctt acccagctta cgttttcacg
    49141 aactaactga agccagtcgc tatcgcctgt taacagatag atgtgttcaa cggttggctg
    49201 cggtgccatg cgggatacca gcagcccggc cagatcatcc gcttctgcat cttttgcaat
    49261 gagttgggta actccaagcg cggtcatcat tttgaggatg tatggcttct ggatagcaaa
    49321 gccttccttc atctttttca tatcaggatc gtcgtcgcga tttgctttgt agtccgggta
    49381 aaagtcgcga cgcttgtcac taaatccatc ccaaagaatc ataggtcggg catggaggat
    49441 ggaggcataa cgacggacgt tcttaacaaa gccgaaagca gcctgtactt ccatttcgcc
    49501 gttgtgcaat ttgtcagatt gttggtggta ataacccagg ctattgccat ctacgaagag
    49561 ataattcacc ggtacattcc ttccaaaaag taaggcgtcc gtagacgcct tactggtcac
    49621 gtaatgggat tacagagagt ccagctctct cagtaggtca tccagacctt catcttccgt
    49681 cgcagatgcg gttgctacag atgtcgctgc gactgcttca gactctttga ctgatacggc
    49741 aacggcagcg gaacttgcct ctggtttaaa ttcagtttca acggcacgga ggatttcttc
    49801 atcaaccaga ctggttggtt cagaagccgg tgtgtgcgcg gttgctacag ccgtcgctcc
    49861 ttctgtatga ccagtgacag agccaaatcc aggtagtgcc gcagccgatg ttttcgcggt
    49921 tgaggaaatt gctggtgcag atgctgcggc agttggtgca gcgatgccaa tcagacgacc
    49981 catagtgcga actgtcgaca gaagacgagt ttcatcagcc tgatttgcgt atgcgatcag
    50041 atcatgctgg gtgttccaga atttatcttc gatatcgcct ttgtagactt tacgcttagg
    50101 cgagacgtca tatttggtat cgcgaccaga gccagtacgt ttaatcagga atgcgtagcc
    50161 ttcctctttg ctcagtggat tgccgatatc atcagcgatg tcttcggtga ttgctttgca
    50221 gatatcatcg aatacagtag acggcagctc gattaactgg catttctcag catctgcgaa
    50281 atcctcacga gcagaaagaa tgccgttgac caggtagcga ggagtggcac gcatttgacc
    50341 gatgcgttct tccattgctt tgttaccctt gtgacgagcg cgaccttcca tcaccatttc
    50401 gcatagctga caagcgcgac cgtgagtatg ttgctcacaa atataagcgt tggttgcttc
    50461 tttaccttcc tcgttctgat acttaacgta gtgcataccg aaagtctgga agaacttacc
    50521 gtttgggtcg tctttattcg ggaagatgcg gatatagttg acgccgtctt ttaagcgagt
    50581 cagatcaacg ttgttgccac gtttggcggc aatgtcttca cgagttttgt taagcaaatc
    50641 aagtaatgtc ttagacatgt gtttctcctt gttgtgattt ggccgatggc gctatgcgcg
    50701 ttgggctttc gttcattcgt ggctctttcg agctgttaaa tgatagatca gtacttactt
    50761 attatctatc aaaaatcaac ggggagtaat aaagcgttca gagcctaatc gctctaattc
    50821 aacgatggcc atctttgacg cctgaacgat catgtctcgg cgatgcgaaa aggcggcgac
    50881 agcatgtttg tatatgtctg cgatgtgccg tgcttcatcc aacttctgcc gtttagacag
    50941 atactgtggg tttgttttaa ccttagcgtc cagtacagac tcgttgaact taatgccgtt
    51001 catactcaag ttcttacgct ctgtgtcgta tagctttgct tcaacagctt caaggttaag
    51061 ttttgcctct gcgacatctc gatctgcctg cgctagtttt gacccatact ccattaaaag
    51121 ccgtggctgt ttacgccata cttcctcaag gttgtcgcgg tcgaactcca gatcggccat
    51181 gattttagaa aaaatatcag tgttcactct aataggttcc tacttataaa atctgtatca
    51241 attatatcaa acagagaacg ggtttatggc gtcatgtcaa ctttgttcag gggaagatat
    51301 tgagatcacc gaagataatt agttgtctta ttttggtaag ctgtgctgac catctttagg
    51361 tcacatacat gagatggatg gtggtgtggc atgaacggat gaaattcagt cgccgttgac
    51421 agatgtcaac actgtgaata gaatactatt atcggaggcg aatgaagcaa catccgaata
    51481 agcatattca agcagccatt gactatgcat taagtaaagg ctggatctgg gtagctgctg
    51541 gtggtgctgc acattgtttt tgcagactgc gatgcggtaa tccagaaggt gaacacaaaa
    51601 ctcaccaaat gagcgtatgg tcgactccgg gtaatcccga aaaccatgcc aaacagatca
    51661 aacgaatggt tgatcgttgt aaatgatttc aaggcggcat tagccgcctt ttgcagaaac
    51721 tgtccaacta tcttcaaagg aaaactatgg cgctttataa cttcactctg acgctctcag
    51781 gcgtatcgta tgagacggaa ggactggaag acgcgctgta ccaaaacggc tgtgacgatg
    51841 cgctaatttg cgcatatgga aactccgtct atgtagaatt tgaccgcgaa gcacaatcac
    51901 ttgatgcagc tatcgcatct gcggtcgaca atattgaatc agccggtatc ggtgcaattg
    51961 ttgaatccgt cgactcggct cttgtgggct taagcgatat cgcggaactg acagacctgt
    52021 ctcgccaggc agtggcgttg ctcaaagacg gtaagcgcgg aagcggcgac ttcccttgcc
    52081 ctgtacaacg cataaaaggc cagtctcctc tctgggattg ggctgatgtg gctgaatggc
    52141 tcatgaataa cggacgactt aaagagggcg atcaaattgt ccaaaatgcc cgaactttga
    52201 gtaaatggaa tctggcgctt cgaaacagcg tctcgaaaga tttcaacgaa atagagcaaa
    52261 ttgcggagaa actgattaaa cgccgcaaag atattgcgaa atgcgcataa tgatgcccgc
    52321 caattggcgg gcatctgttc tctgtaaagg tgtctggcat cgtctggttc gttaacgttt
    52381 actaaaccaa tggtgacaaa aattagttat ctttgaaaaa taaaggttgc atgaaaagaa
    52441 ggggtatgcg ttaatagcat cgttggtttg caagacagac cttatgtaag cagttttagt
    52501 aaagcagtct cagttcagtg ttatcttctg atttcccttc ttcgtgagtc tcctccttaa
    52561 gtgcctaagt aagtcccaat ctatacagat caaattctcg ccgcactatg tggctcacaa
    52621 aattaagaag gtaatatcta tgtctaataa aatgactggt ttagtaaaat ggtttaactc
    52681 tgataaaggc tttggcttta tttctcctgc tgatggcagt aaagatgttt ttgtacattt
    52741 ttcggcaatt cagagtaata atttccgcac tttggaagaa ggccagcagg tcgaattctc
    52801 tatcgagaat ggggctaaag gcccagcagc agcaaatgtc gttgctctaa gctgattcaa
    52861 cgctgatgat cctcatttct acaataacga agacggtgta agcctgagtg gatagatttg
    52921 taggattgta caagttgaat aaattagcat ggtaaggcca tgctaattaa atcgacacaa
    52981 cggccagttt attccataaa agtgaattgc ccattgtggc gaaatgcggg gttgccgcat
    53041 gaatagtcaa aggcagaagc taactgccgg catggcaaaa caaagtgcgt aagaggagtg
    53101 acttcctcaa caagcgccat aattctttat aaacccgcca acaggcgggt tttctttatt
    53161 acttatttat tgtctccgcg atatcagcca gaatggcctc cagtcgctct ccttcttccg
    53221 gtcggaagta caggatattc gggttaaatc cgtagaagac ggttgcatca agctctggga
    53281 agtactcctt acgaccaatc agatctgatg gtttactctt attgttaaat agtgcggttg
    53341 aacgactacc acacgtcaaa atgtaggttg gtcgtacaag attaatttct tcccgcataa
    53401 agtctgtgaa ctggcctatc tcgtctttgg tatagtcttt ctctttgtcc ttaaccttct
    53461 tacaaacacc cgtgacataa agatcgccca tgcgcagatc tccaactgtc agtagttttg
    53521 ccttaaagtc gtcgtatccg ttctccatga agtagccggt acgagcgtca ttgccgttcg
    53581 cattgtccag aatgatcatg attttgggtt taataccaat agtcgggcgt atcaactcct
    53641 cacccaagcc catttcagcc gccatacgtg tcatgagtac gttgatttcg gcagaacgct
    53701 taggattcat ttcgaacgga cgtgatgctt taacagcgtc aatgatgaga ttgcccatca
    53761 gctcagcctg gtcgcgcagt cgttcaggat cagttgctgg cagactgcct ggctcaattg
    53821 acgcaaaagc ccctacttta tgaagcgact cgcgtacacg actgttacat gcacgcttct
    53881 cgaccgcttc atcgaattgt gccactgact cgaatttgcc accaactttc tcacgcgcac
    53941 gcataattgc ctgacaacca ttttcagaac agcctttcac agcagagaat ggcgcataca
    54001 gtgcctgact gccgtcttcg agtgtgcgga tctcaattcg gttagatgac acattaacgt
    54061 ctggtggcaa tacgcgaatg ccataggtca gcgcatcctt caccagcccc tgatgcttat
    54121 cctcgcccag aatggtgagc gcagctgcga agaactcagc agggtagtgc gtctttagcc
    54181 acatagactg ataactgatt aaagaataag caacggagtg tgatttattg aactgatagg
    54241 ctccgttttt ctcaaatgca gtccatatct cttttgcctt cgtctcagac agacctggat
    54301 gtgacgttgt aacgcgcaca actggcattg gcagcttcac tccatgctcc aacgcctctt
    54361 caactgtcct cagagtaccg tcctcgcatt taaaatgttc tgcgcgatga atccgttgta
    54421 ttgcgccatt ttcaagctct acgtcgatcc acccagcttg agcctgaatg acaaatttct
    54481 cacccatgct cctcatcttt tccatgttct tcttaccgat tgctgaacgc acaccatccg
    54541 cctcggccat tgtgaaacca gcaagaagcc ttgttgcgtt catcgtttgt tcctgataga
    54601 gaatcactcc atttgtttca gaggtcagtt catccaagac aggatgtagt gatgtaggcg
    54661 tcataaatcc tttcgcaacg gaaacatagt catccaacat gccggattga atcggtcccg
    54721 gtcggaagag ggcggtagtt gcgacaacgg ttttgaaact cattggctcg atgccaccgc
    54781 ccagatcttt aagcagctta cgcatggggc cggactccag ctggaatacg ccctgcgtgt
    54841 accctgcagc gaacccatcc agaaccttgc gatcgtccag tgggatagcg tcgagattga
    54901 tgtcttcacc ggtactctct ttgatgtagc gtttcgcgct atccagaaga tcgagtgttg
    54961 ccagaccgag aacgtccaac ttaatcagcc ccatcgcctc gcagtaacgt ttatcgaacg
    55021 caatacatcg agcattgcca cgcagctcga cgggcgtgcg ctctactaat ggaacgccag
    55081 cgacgatcat ccccgcagcg tgacgaccaa aaccacgcat caggctttgc agcttacacg
    55141 ccgctttgaa cgcttccggg tttttcgtgg cgtatttgtc caggctggcc agttgctcgc
    55201 gcagctcttc cagagacagg ctgtcgtcct ccatattctt gaactctttg gataccgcca
    55261 tatccgcagc atcgacaccg taaatacgtg cagtgtcacg cagcgcagat gccgcgccca
    55321 gataggtgaa gttcggaatg cctgcaacgt aatcttcgcc gtagcgttca ttcagatact
    55381 cgatcacctc atggcgacgt gcctggctga agtccaagtc agcatccggc aagtcgagac
    55441 gctcagggtt gatgaaacgc tcaaacagaa gaccgtgacg gattgggtcg acgttggtta
    55501 tgcctatgca ccacgccacc agagaaccgg cagaggaacc acgaccaggc ccgacaggaa
    55561 tgccagtttc acggctgtga ttcatcagat cgcgtaccat caggaagtaa ccacagaatc
    55621 ccaggcgagt cagcgtgtcc atttcgtact ttagccgctc aacataaacc ctgttctcag
    55681 aagcaggtgg tgtgtatcca aactcttttg tggtaagacg tttacgcagc cctgcaacag
    55741 ccagtttcat cagcgttgca ggttcgtcgt ctgccatctt gggcagtgct ggtggcaatt
    55801 catgccagcg ccatgtgcag gcatcgataa tggagtcctg cgttgttgag gccattgcag
    55861 ctgttaccgg cacatccatg cgaacggaga aggctttaag cgcctcaagg agatggcgac
    55921 gaccattgat ggcgttatct cgctggtggg ggatacgcag acgatgcggc tggtcgattt
    55981 ttatgttgtt cgtaaccatg tgcgcaatgt ctttaatgtc tgcatcgtcg atcgattcgt
    56041 aataagcggg atagaacgcc actggctcta ttttcaatgc gctggcgact ttcatcgccc
    56101 ggacgttaat ctggtcgtag aatggggtag ggtgcggata aaccacacta tagaagttgt
    56161 cttttccacc cgctgtaatc agtgtgctta tgatttttgc aaaatcgttg cgttggaaca
    56221 cgctaccgat gtcggaagta agcaggatga tgttgccttt ggcatatgta gaaaccaact
    56281 gttcgagcga gagacgcggt acaaagtaga actgttctcg tgtgttggcc gccgtcatta
    56341 gttcgcagat gtcgcgatat cctttctcat ttttaattag agcggtaaac gagtagttac
    56401 gtcctcgctc caatgattcc atacatccct tagactcttt agcaagttta gcccggtgct
    56461 cgtatgtggg atcatcaacg atgtttagtt tcacaccaca aataaccgcc atgtcgtcac
    56521 cagcggcacg ttgtagtgga ataacgctcg caatattcat gctatctgct gaaattacag
    56581 cggtgtagcc agcttctctg gcaatcttca ccgcgttttc tgcttttaaa gccgactccc
    56641 caagtgagaa gtcagttcga accatcagtg ccttcatgtg tttttacctt tctggttttt
    56701 ttgattttgt catttgggaa gcctacgaac ttcccatgca tcgaaatcgc aacttctttt
    56761 gctgattggt gacaatcggg cttgtctgga cacaccagac aagccttacc agtttcagaa
    56821 gcagcgataa gagagccgaa acatccttta cgcacgatca accaaatatt ttttgaacaa
    56881 cttcacgagc cgcttgtgca gaagctgaag ggagtttgtt aatgaaagaa cgctcaatac
    56941 ctgtcacaaa gtcgcctcgc atcattccaa tcttggccga caacaaaagt tcacgagggc
    57001 caattggctg actgataagg tgttcttcgt atccatcgcg aacgataccg gcaaacttaa
    57061 ccatcttttc tgcgtattca tccacgatac cggcatttat caacatgttg atctctgcct
    57121 ctttgctcat gtatttcaca ttcgaaacga tgccaaaacg cgaaaagttc gcggcgttct
    57181 ggatgtttgt accctggtac aagccggttt catcaccaga tccgttcgtg ttgccagtgc
    57241 caatgaaagc aaaccgttca tgcggagtaa tgcgacgcca ttctggagtc gcctctttga
    57301 tgactaacgc ttcaccttcc agcactggct gatacacgcc aagaatttgt ggaaacgcaa
    57361 agtcgtattc atccgcgagg taaacccagc catgcttcat tgcgagcgca agcaagccag
    57421 gctcgaaata cgtagagcca tcacgcgcca gaatttgccc cgtaacatgt gcctcttcca
    57481 ttgatgctgt atgttgggca cggatcaacg gacgattgag caaagcacat agttgtgtag
    57541 gcaatgacga tttgcctgtt cctgcatgac cccacaaata gccaggtatg ccaagttcaa
    57601 gcatcatgaa aatgtctttg atcagttcga agtcgccata tacgtatccc ttcttcactt
    57661 ctggaacgaa ctctggaaac ggcgtattga cgttgacgct aaccatgagc ggcgtcccac
    57721 gttttgttcc gagttctttc accgttacat ttagcagttc gtgagctgcg accagatctg
    57781 tcttgtactc aactgtgcct gtgtagcctg ggctactggt cacgccagat gatttggcca
    57841 tttcgctttt ttgctcggca cgtttagctt gaattgcatc aagtgccttt ttcgatagcg
    57901 ttggctcatc tggaaactgt gttgtgtaca ttttcagtac ggtgtcagga tcggcatctt
    57961 ttactgactc aggaatgccc tcgcaattac cattggcaac atgggactta aaatagtgaa
    58021 atgattttcc gcaccacttg caaacaaggt aatcagaagg attgttttca ttttgcagtg
    58081 cagtagtagt catgcgtttt tcctttgttt tctaatgagc gttcaacttc ttatataaat
    58141 atatcattat ctatcgtaag tggttactta tcgttaaggc tgttttgttc actttaaaat
    58201 gatacgagat agttcagtaa ctacagatga accaagacta tcaacgctcg ttaccagggc
    58261 atgatttgag tataatctct ccggtgcgtc agtcatgatg ccaattgcca tcaaatcaat
    58321 gtcagtctgc gtttcaattt gcttcgcaac cgaacgcaaa tgagcgtcaa aacctctccc
    58381 aacagcccac ggcgcgccat cactcagaac aagcatgatc ttcctgtcct ccatgcgacc
    58441 ggaaaataac gatgcaaggc tggctatgct ttcaccatcc acgttattaa gaagtgggaa
    58501 tgtgtagcct acgcacccca tacgtgagcg aacttcgcga gaatttacct tttcattcca
    58561 atttttgatg ataggtagca ttaaagattc aaagcgagag aactcatact tgacagcctt
    58621 aagttctcct gccgccatat gactaccaaa tgtagtgaag ccggtgataa tgttgggaac
    58681 atttattcga tcaagggcat cggcgatggt atatgcactt gcaagagcca attgtatcgt
    58741 tataccactc attgaacctg ataaatcaat cacttgctga acacaggcgt taacggcttt
    58801 agattcttct tttttacgaa acacgcgatc atcattcatt gctaatctgt agatactaga
    58861 accatgaatg cgcccccgac gctggcccgg tataaactga actctgtttc tgcttgctat
    58921 tgctcgctcc aggtcttttg ccagcgtcga cgaaacacct gacgaaagat gcttttcaat
    58981 atatttgtcg aagagctggt tgccttcagg aacgatgcga tagcggctga ttggatatcc
    59041 tcctaaatca atatcggaga atgttttaat aagccgtttg atatgggctt ctgcctgatc
    59101 aatcgagccg atgaagtcgt atgagcgatt gtatggcctg tattcgcttt tcgaggtttc
    59161 tgttaactcg cttttaatcg tttcggatag agcatcttct gtcatgcctc cgacttcatc
    59221 ttctatgcta tccagctcct ctaaagcctc ttccagactc atttttgacg gagttgggat
    59281 atctgaagag ccgtcttctg tcgtttttct tttttcgtgg tcagcggtaa atgattctga
    59341 caccgcgtta tcagaagcat cagaatctga tacctcttta tcattttctg tatcatcgaa
    59401 tattgtttct gatgattcag aattatctga atcatcaccg tcgttattat catgtttgtg
    59461 cttactgtgt gattctgatt tgatagattt gtgattatct aacgctgagg gtgtctcatg
    59521 atcttcgttg tcttcattgt tttctccaga ggcatccgtc tggtcagcgt ctgaaggatc
    59581 ggaagttttt cccggagctg actccttacc tttgctttca ctttcagtgt ctttgaggat
    59641 cttagctatg gtcgcagcca cctttacaca atcctcggtg caagacatgt tacgcacggc
    59701 cacatcgata ccatgttctt ttaataatga aattggtttc tcaatgacag gccaatattc
    59761 atccatgaaa tctacgaacg gtgcttggcc atcccaggct cttacaaccg gacagagaaa
    59821 gaagtttaga aaaagcgcgc gctgatcttt gccacaataa gcaatagcct ctgaagcctt
    59881 tggtttaaag actttttcga ttataaggcg ctgagttgcc atcagattac gtcttgttcc
    59941 gttaaagacc tgacccattt ttcgctcgat gaacacgtct tctaaggcat tccatagcga
    60001 ccaggaagga acgcgtcctt tttctctcat tttgttggac acacgaatat cggtaaacaa
    60061 aatgtgagca acctcatgat cgagaaatcc gcgcacagca ttcatcaatg ccggtgttgc
    60121 gttatccggg attgatggga tgtttacgag aattggctct ccatcatcgt tatagcgtac
    60181 ataagcgtcg tcccctcgtt ctgcaacagg gatgttttta cctgaaagga gagcgactac
    60241 tcgttttaca ctgtcacgga agtcctgaac ctctttgatg gaacgttttt tagacatggc
    60301 taatcctttg ttatgaaaac aaattattta ctagtgtgtt taatgtagca ctgcgcgaac
    60361 agggaactaa gccattcgcg cagggagaag aggggttaac agattctgac ggctaaagac
    60421 ccggagccgg tattgagaag cgtgaagcgt ttgttgttaa gttcgaatat aaatccagtt
    60481 gtgtcattca caccaacctg aatctgctca ttcggtagat cggtgaggat gtcagctaca
    60541 cactcatcag ctaatttctg tacgtgtccg atctcaagag cgattaggct ggaaatagtg
    60601 gtgttcattc aatcaatcca ttctaactta tactatggta aggaatacta ctaaaatttg
    60661 tatcaatact gaatacattc atatcatatg caagttgatt ttcttaccta ttttgggcta
    60721 atttttctcc gtgcaatggc ctttaaccgg tcttttaagt gcctatcgaa aaaaatgata
    60781 atagctttac aaccctaacc tttgatgtaa tatcggtaag cacttaccag agagaattga
    60841 acgcgcaaag gttgtaacca tgtctgataa caagattgaa tttatagaaa gtcgctacgc
    60901 tgcttttatc gcagggttga ttgaatcctc acctatgagc caggcccaga tagccaaaac
    60961 aattgggtat aaaaacgcca acaacctttc tttaattaaa agcggcaaaa ttcctttgcc
    61021 tatcgataag gttcgtccgc tggcgctggc actgggtatt gagccaagtc gtcttatgat
    61081 gatggtgctg gaagaacgcc aacccgaact cgcagcattt ttatacaaag aaggcactgc
    61141 tcctcttaac gaggacgaaa aacaggttct tgctgcatat aacgagcgat tcggtaaaga
    61201 gaaaggcgca tcacaaaagg ttgttgaggc cataaagtct ctatgaaaaa tttacacgaa
    61261 taagctctgt tgatagacga tctcccttaa atttgtggtc aatttcgtct aaatccggtt
    61321 gctctacgat tgatgcgatg tacgtcgaga aactttctaa ggcgtctcgc atctcgtcca
    61381 tataatcgtg ccggtcgtag acccgatcta tcccctcaag actgtggttc atgattttac
    61441 gtgatacctc ctggcttatg cctaatgctg ggaagtaact acgcgcagta cggcgcaaat
    61501 ctcggggtgt aaatggctca agctccatca gttctggtcg ttccagaata cgacgtaatg
    61561 cctgggctat tgccacttta gacataggaa ggtctttccc gattttttta ttcgaaggca
    61621 cgagccactg gctgtcttta ccatattcga acaactcttc aatacaagtg cgcattaatg
    61681 agcttaaagg cagagaatgc tcacgggcag atttgttcct cttgccttga ttccaaaccc
    61741 cacgcttaag attgaactca ctttttttag cccgcagtac ttcgtcaggt cgtcttgcgg
    61801 atacaagaca tagcctggcc gcccattttg taccagcaca cacattgaag tagtcccata
    61861 tattccagaa cacccatacc tctgcgtcgg tcagcttccg ctcgcgaggt gtgggctttg
    61921 cgccaccggc aactttgtta agtgacatat cgtttaacgg tgacacgtcg atcatcccct
    61981 ggaaggcgca ccagctgagg aactgcttca tcagagagaa aacgcggcga cccataacaa
    62041 ttttgccatc cagtattagt gggttgacca gttggttcac catgaaccta ttaatatcac
    62101 ttacttttac atcggcaatg tgcggcaaaa catgtatcaa aatacaatga acagcgatct
    62161 ctggccgacg tcttgttatc agcagagata agcgagtgaa taacatgaat gcgtccgaga
    62221 atctcatgtc attgctgacc tgggagatca taacggcctg catttgagat gctctttcga
    62281 gatactcaat cgcctctttt gaggtgttct ccgcagcgcg tgctctgtca aagctatttt
    62341 tcatatgaca atcaccgagt tacgccgatg tactgtataa gtaaacagta tattaggcat
    62401 agattcttat aggatcaaga gtaaaagtaa ctcattttta ctaatgattc catacatagt
    62461 aggtatggaa tcattttgac gttgtttttt gaattttaag ggaaacggga gtgagtttgg
    62521 tgggaataaa aacggctcct gtgagccgtt aaaatcagag ggtgctgacg tatgcagcca
    62581 gttctgtgta tccgccaatc ggttggccat cgatcagcac ttgggggata gtctctaccg
    62641 gtttaccaac aatttcactt agtttttcct tgtcgatacc ggcagtggtg atgtcgatgt
    62701 actcataatc accgtagcca tgtcccttta gttgcttcgc cagctcgacc gcacgtttgc
    62761 agaatgagca gttatctcgt ccgtagatag tgatcgtcat tgctttgcct catgccactt
    62821 ttgcttcagg agatagcctt ccaggggcca gatttgatcg atagcatctt ttcgagcgta
    62881 ctcgcgacca acatcaaagt tgaagatttc tggtcttacg cacgcagatt ttcccgttac
    62941 ggtatatcca tttttgagaa ccataacgca gaacgttaag agttttaacg actcatgaca
    63001 tttgtcgata tctgtttttg aattgtaaac ggagatgaag ttatccccgt ggctggcatc
    63061 atatgcagtg aaataatgtt cactttgaat gagttgttca atgtgttcag gggtgacttt
    63121 tagtggtgtg ttatctttgc tggtcattat ttcttcctac aaagttcgta gacttgactc
    63181 tttcagttgc aaaaaagggg ccgaagcccc tttgaatttt gcgctgaaaa aaagtgttgc
    63241 taggatgtta gcaagctatg gttaagaacg tctgccgtaa ttgtctatca cagcgttgag
    63301 cggtctggtt tatgttagcg gagtcttaat ctgggtgatt aaaaggccaa accgctcaac
    63361 gctgtgtctg gcggagagta atggaatcga accatcatcg cttgcgcaat gggacggttt
    63421 tcaagaccgc ttgagcacca tgctccctac tctccggccg ttgtggtggc cggtactgaa
    63481 tctccggcat acggtgcagc caattaggac tacggacgat caccgctcgc gaaagggaag
    63541 atttgcggcc gcatttccct aacatccaag aaagctatcg catcagtctg cgaatccacc
    63601 acaacggtaa gatcactacc tttcgaccag tcattgtgca catgaacgac gattaaccgt
    63661 aatgatctta tcgttgtgtt gtgaagccag atgcttatct tctggttgct tcaaagagct
    63721 gcacttcctc acaacggtaa gagcactggt ctgactctat cgatttacgg gcttgtgggt
    63781 cagtatcgcc gattcgccca atgctcttac ctgttgtgtt ggtgccggtt aacgggatcg
    63841 aaccgctgac atcctgctta caaggcaggc gctctaccaa ctgagctaaa ccggcaattt
    63901 ggtggggagt gatggagtcg aaccacccga gtcgcaatga cagtagattt acagtctacc
    63961 ccgctacctc tacggactaa ctcccctaaa ttggcgatgg tgggtggatt cgaaccaccg
    64021 accagttggt taacagccaa ctgctctacc gctgagctac accatcaatt cagcactgcc
    64081 agtatttatt actcaacagt gccaatcgcg gcggtgttag tttctgccag gaaacacacc
    64141 gctacttgca ctttccgtta gtgccagacg ttttcgactt cgctcaatag aaggcaaaat
    64201 ctttaagtag atgatgtttc tgaggacagc acctactttg taatttatac aatgtgatgt
    64261 atggaatcat ttgttgtgaa aacaggcacc aatggcaaca gtggttaaac ctacacaaca
    64321 atcctgtctt cacaacgttg aggccactac tccgattgaa tgtttgccca acatgttaca
    64381 tatcaacgct cgccggtatc tctgtgaata gaaccttaaa atgtaatggc ctcaacgttg
    64441 tgtgctggct aaccatacca gccgggctac gtcgccgctt tttaacccag tataaacgac
    64501 ataattgaac aaaatgacgt aacaggatgg gcggtcagtg gctaagaatc cgggagtcat
    64561 atggagttga aaatatacca accgcccatt ctgttacttc atcggaggga acatgaatgt
    64621 tccctcctgc gttctgcaat cacactcgct cagtgtgtcc catttcggta acgaggctgg
    64681 aaactgacct cgctggtgtt tggcttatta ggctactgcc agatagactt cttcgtttgc
    64741 acttgtattt aagttcaaac agtcgcgtct caacgaaaac aaagtcatct tatacataaa
    64801 agataagtaa gtaaatactt atcaccatgt ttttattcgg ttgtgacttt tttgatcaag
    64861 gccactctac gactaggtgt tcttgtgatg gtggcagtga atcgtttcgc ttggatagta
    64921 atggtttcgt tctccttaag ttgcccgtag cgagtttcca ccagggtgcc aagacgccac
    64981 agaccatcat cgatacgttt atgactggca aatttaatca gcagcagttt tacgactaac
    65041 tgacctacgt aaaaggcgaa tgcgactccg gccgctaaga aataggtggc caaccaccag
    65101 tcccaagaag ttaaattgct cattttctaa cctttgtttc atgaactacc cgatacacac
    65161 gctttccgat gcacagtgtt ttggttttaa gttcctgctt aattaaatca cgacagatgc
    65221 cgaagccgat aaggaagcca cccataaaag ctaatgagat gtatggaatc attttaacgc
    65281 tcctgattct actaattgct gtagcaatac tttcccttta tcagttaact ggtagttggc
    65341 cgaacaccac gtttcagaaa cgtttgcaac caaccctaac cgctctaact tagcccgcgt
    65401 tttaggcttc cagtactcag ggaactccgg ccacttactg atttcatgaa gcgtttcctt
    65461 ctcccgctta cttaatacga tcatccttaa tctccttcac tgtgcgggtt atttctacaa
    65521 tgcgataaat acgaccgcgt cgttctataa caccgctttt aatgtagtca ttgatgcaac
    65581 tggacatcac cacaataccg attaaaaaac caaccgctaa aaatgcaatc atccagccga
    65641 gcatcattct ttatctccga ttcgatcttc tgtatcgcgt aagcaacgcg gccatttcag
    65701 tcgtgggtgg cgtaagctgc cgtccggtgt tttctcgtga caatgaacct cgacaatacg
    65761 accacggtac ttctctttgt tgttccagat ctcgtccaga tatttatgct taatgccgct
    65821 cgcacgaacg atgacgccgt tttcaagacg aataacaatt tttccaagtg tgttggcaaa
    65881 accagagtcc gggtcgcccg gctcaaagtc gataatttca ccatcttctg aatcttcgtc
    65941 ttttaacttc caccagctgc gggtacgttt aaactcgtaa acagagttcg gatctttgcc
    66001 catctcccct tcttcgttat cgtccaggcg tttcatgaag cgttcgatga agtcttcgtg
    66061 gctatggatg atgtagaacg gatgtaggtg aatgtcttgc gcgtagtctt cactgcgatc
    66121 gtttttgaac aacgccacga gaatagccag gcgctctttc agcttcatac ctgtctttgc
    66181 atactctttg gatttagcct gtgcccgcca ttccggtagg aagaagtcga aaacatgata
    66241 aatagcgcca attgcctgca cgttcttttt gcgcaatgca gatacggact ggttaaatga
    66301 accggctgtt ccttcaccat cgaagaagat gtgtttgtgg cctgaaagtt tgcctaattc
    66361 gagcatggct ggctttaggt gatcgagaga cgtgattgga ttaccagtac gagaaaggaa
    66421 gttaacctct tcctcgtcaa caataacctc gcaaattact cgcaaaccat cgagcttaag
    66481 gctgccaatc atcggccatt tggctttagg atttggcttg aaggggtatt tgtcgccttt
    66541 ttctttatac ggtgacgcca gctgcacctc gaatttcgga atgggatttt caaaaacctt
    66601 gttgcatagg ctaatcccaa cacctgcttt cgggtctttt aagaggaaac gacggaacac
    66661 gtcctgccca tcggcgcaca ttgacgccac gatagactca acagctgcta ttgctgcgtt
    66721 ccccgtcagc tcgcgagagg ccagcttatt cagtatatca atggcttttt cgtcacttgg
    66781 gacggactca ctaagcggct ctgccacttt gtatttcttt accccaaaac ggatgaaagg
    66841 gttgagcatc aatgaaacca tgctttgctc gaagtcatcc atgttggcca atgcctcttt
    66901 ttttgcattg gttcccatag ctttcatttc gtccagctta tgcttaagtg cgatcaactt
    66961 tttcattgtt gttttgcctc catatgttga tcgatttgct catgtgtttc ttttgttgtt
    67021 tcttcaagca acgccgcata cacgtcagtg atcgctgttt gtgatgtggt gctcttctta
    67081 accatgctat taatcgttac actgtcacgt tttcgtttta tagttctggc ttgtttattc
    67141 cgttcttcaa cctccttaat gagcgcggtc atatcttcga aatacagaga ttctccttta
    67201 cgaatctctt caaccatcat ttttaacgcc ttgcatttgc ctgctttaat agcggttgcg
    67261 cacgactgga aagaagttcg aggaagacgg ttttctttga aggcaagaat ggtgtgctgg
    67321 caaacggaat agctgcaata tgccgactca ccgtttatct ttacttcctc acaacgaagt
    67381 gaataaccgt tatttcctga aatagaaggg atttttgaca aatcagcttt cacaactacc
    67441 gccataaata atcttgtact tacctatcgt tatttgcgta aaaaacgacg cgcggagaga
    67501 ttaccaactt ccccagctaa ccatcttgcg ctgattgctt tctaagcgat aggggacaag
    67561 aagctctgtg acatgattgg tggcgtatga tttggcctct tgctctatca tcggtaattc
    67621 gttagcaatc ctgaccacct tcccagcaaa ttccgccatt acgccatcac atgccttccc
    67681 agcatcaata acgatatgca ccagatccaa ctggcttttg cacatgtccc aaagagtcga
    67741 atattcgctc tccctgatta catttacggc atcattagcc cctttattaa gcaagacttc
    67801 caacaggttt gtcggggtaa cgaaatcggc tttaagtgac aatgttctct caccttttat
    67861 cgaccgtaag tactcttcgt agttttgctt gtcttccagc aaaactaagc tatgcatagt
    67921 tagaagtgat tcttgaatag cctcttcaat aatactgtca tttgacgcac aaaaaacggt
    67981 atttgtgtag atcactttgc catccatcca tgccccaacc tgcactttaa gactgatcgg
    68041 gttttctttg ctaataaaaa caactaatgc ataacgccgt gcacaagaag gttcacccca
    68101 tacgtgtaac ttaaccttca gataaggtag ccccggtagc gggatctcga ccaaacgagt
    68161 ggcgatatga tccattgctg tttttacggc tttttcaatg atgtctagcc gattgcactc
    68221 gctgatttct aaaccagttt tatcaatgat gtcgcaggct aatttctgaa tttcgtcttt
    68281 cataccaact cctagctaac agatgaaagt attcttacag aaagataagt aatcatctac
    68341 ttattttagc ggcacttgtt acgatagcgc cttaatgccc aaaaccttgc tttgcaactc
    68401 caactgtttg gtatacggtt ttgcccgata ataggctttg ataatttgtt ctggcgttgc
    68461 atcgcctggg tcgagtcctt cctcgcccaa acatgctact ttgacattaa gaccaatact
    68521 ggtcagtcgc ctggctgcgg ccatagtgtt gcgtatagct tgcttttcac tatcccacat
    68581 catgatcaca ttacgtaatc cacgcgcctt gagcgtcagg aacgcgccca actgatcttc
    68641 tgcatcctga gtggtgttac cagatagatg catcccgaac gttcctatcg gttccacgta
    68701 atcacgtaat gtttcttcat caaaaatagc tcgtttaact cccataacat cgaacgcccc
    68761 ctcacagaca actacagtct gtttgccgac cgcattatgg ccgttgtaga gaaacttacc
    68821 tgaagctgga agctgcatag gaaagaggta gcgacgttca gctgtaccgg taatgtctcg
    68881 cccctgaaat gtcttcatta ccccatccag atcgtaaacc ggtatcagta ttcgcatatc
    68941 gaatatctgt cctttaacct gatctgtata cggatctaca taagcgtgct tgccttcgac
    69001 gcagtatcgt aggtcaaaat actttgccat ttcaggcgat atgtttcgtt caaccagata
    69061 gtctggaaga cggccatcta taggaagttc gtaatgacga gggagtgcaa gtggtccctc
    69121 taactcgact gtgcttgcaa gcactatctc ttctttcttt ggtgcccacc cctgggaaag
    69181 aagggcgttc tgcacatatt cctcgaactg tcgtcgtgat ttgccgctgt agtgcttgag
    69241 gaagaccagc ttattgaact gaatctcttc gggatgatca ccagcgaagc atttaccgac
    69301 gccactggtc agattgaaat ataccttcca gttggagctg ccacataccg gacactcctt
    69361 gatattcact tcacgaccgc gagtactcac gcctccacgt cgataaacga taccttcagt
    69421 atccaaccat tgttcaaaat ctaattcggt aattagctct ttcagctcgc tcacgataat
    69481 tccactttta acaggcaata ttgtgaccaa cctaaatgtt gatataacat aaaggctcat
    69541 gtgtttttct tttgtggttt ggcaaaagaa aagttgtttc accaatgaat caagcgtgga
    69601 ggtgttctcc acgcttattt tttaggtaac gtctaagatt cgctcaatga agcgcatttg
    69661 ttcgaggttt tgtttaacgc gaatgctgat ccctccctgc tggttacgtg aaccagcaaa
    69721 gtagagacga gcctctcctt tcgcttcttc ttcttcggtt ttgttgatcg ttattaccag
    69781 gtcagcaata cgtacttttt cgatattgtc ggcagcgtgc atcattgtgg caacttctga
    69841 cgcgccacct tcacggtttg tctgcgatgc cgtgatccca gcaacgttat gcttgtcata
    69901 aagagcacgt aaatcggtgt agatactacg aatgttggcg cgatcatcac gaaggtcata
    69961 actggcacgc atcaaatctg cgtagtcgac aacaaccatg tcaggcacca tgccattggc
    70021 tttcatgctg ttaagcatac ggtccagatc tgccggtgac atacttcctg acggacgctc
    70081 aacaacccac aaactaccaa tccccttcgt ggctcccaac tctgccaact tacgatgaac
    70141 ctcatcgcgc cgttccacca gcttggacat ttctgtctcc gacaatcttg catcaaaacg
    70201 gtcggataaa atggtggtgt gaacctccag cgagagatac agaacattgt agccagcaag
    70261 cgttgcgttt atggaaaact cacccattgc ggtcgattta ccggatttag cgaaccccat
    70321 gaagagcacc atttcacgct tcgcccagcc tttttggtac agcaacctat cgagcagagg
    70381 gagtccagtt gtaatgctgt ttggcacata ctcctctgaa gcctcatatt cacgcgcttt
    70441 caatcgctca cttgcggagg tgtagtagtc atagattccg gtcgcttcgt tcgatccaat
    70501 ctgctgaacc ttggccatga ttgccatcgc cccctgaaag tcgcccttct ctttcagttc
    70561 agcagcctta atcagagcat catcaaacgc tacgcttttt gcgaaggttg atacctggtc
    70621 aaccatgtac gaggtatcag acaatttttc tgcaaggatg cgcttaaacg cagcaacaac
    70681 atcggcgaat agttcctcac ggatagtctt atcgcgtttc gcacgcttaa gcatatccag
    70741 aatcgcagat gaagagggcg cgctcttgta catacggtaa tagcccgaaa ccatattaac
    70801 caatatggca ttggccgcat tggcaaattg gctaggtaca accagatctc cagcacgagt
    70861 aagaaactcg tgatcacgac aaaaataggc cgtcaatctg ttctgaaaat cttcatcaaa
    70921 ctcttcggac agcccgcgtc ctgtatggca aagttcggtc atgtgctttc ctttgttttt
    70981 taaacaaatt gttttctagt attagttaat tagatagggg atcaataaac cgccgtgctt
    71041 cttccagttc ttctggaaag tgggcggaaa taaggcgctc tggaacgatt tccattagcc
    71101 agatagcgga gaaaattgcg cgtatgcgct tgctgcgggg gatggtgcgt aaacgctcca
    71161 gaatccactc aaaatagctt tcctgaatcg ggttgaactg catgtctccc atatgcttaa
    71221 agctaacgag agagtcatcc agacgggttg ttgcgcgtct ggctaatttc tcttcgaata
    71281 tctcaatcag ctctggctgc cacaaatgct gtgggcgcgg cagcttgtcc cacagccgtc
    71341 gtgcagctgc ggaaagaacg gtggagataa agtagtcgta tgagcagcaa tagcgatccg
    71401 caaactggcg tgctttccat agcgacgttt tattggcagt cgacaactcc tgataaggca
    71461 ggcgttttaa cccggtggtg aacggagctg tttcaaagtg ttcgcgaccg tgcgtcagca
    71521 tgatatttga gtactgacgt ttgtatgcct ccgtaaaaag acaggtggcc atgagaggat
    71581 gcatgtcgcg gtaatcaaac cacttcgtct cgaagagttc agcctcgtct ttacagcgcg
    71641 acaaaccaat gttttcagcg acccacttgt ccataacagc ggtattccac tctgtcatga
    71701 agtcgtactg gtcgttgtcg atggtatcga aaaagatttg gctcatgtgg ctcacctggt
    71761 aggtagttac ttacttatca caatgagcgg atgatagcga ctggtggcag tttttggaag
    71821 tggaaacgga agggagttgt tctggtggtg tcttttaaaa gacctgcttc cgtatatatt
    71881 taataagtta cttattattt atatatacag aagcaggcat aacttgtcgt cttagacgcc
    71941 cagaagctct acactttgag cctgttaaat tgattaaaaa cttatgtgta aaatagaacg
    72001 cattagtcag tagcaaagag atgcaataat ggacgattta tcggtatacg cgagatcatt
    72061 ggataaggct aaatactatg tttactgtct ctatgataca gaagacaaga tgaaaagacc
    72121 gttttacata ggaaaaggca aatcgactcg ttgtctcgat cacatcaagt atcccgatga
    72181 ctctcccaaa tccatgcgga tcaaagaact tttggctaat aaaaaattag gcatagatat
    72241 acttcgtcat ggtatggatg aaaccacagc caaacttgtt gaggcgacat gcatcgacct
    72301 tatgggggtc ggagagctta cgaataaggt acgaggaagt ggttccatga tgggcagaat
    72361 atcactggac gcctaccatc acttagtgct gcaagaggaa actgaaattg ctcctgagca
    72421 cgccggtctt gccttcctgc taaacagcac ctacaagtca ggaatgtccg ctctggcatt
    72481 atatgaagct acgcgtggcg tatgggcaaa ggttccaaga gacgagaatc tcaaatatgc
    72541 ctacgctaca tatggtgggc tgataatgga agtttatcag attgaatgct gggtcaaagc
    72601 cggttctcag cagtacttta caagggatat agctcttggc cccgacacaa aacgttatga
    72661 atttgttggc agaatagccg atgagcatat tagaaaactg tatgtaggca aattaatcaa
    72721 gaagccacca agctacggta gcccttttgt gaaggttggg gtggttagga ctgaacatag
    72781 tgttagcgcg gcataaagct gacactgtag ttatgcattg gcgcaatgta cgcgccaatg
    72841 catgattttt aacctttctt catcaactct cgtttgattt catcggtacg catcgtgaca
    72901 tcggcagcgg tgatcgcctc attcaatttc acgatgtcct cgatttcctg cggtgacttc
    72961 tctgccagat ggaaaatggc tgctcgaatc acgtcagaac gagtgaactt ctcgaagcga
    73021 gggatgaact tcatcatctc cagcagttcg aagtattcgt cctccagtga cattgtgcgg
    73081 cttttaattt tctctttgcc acgagtcggg cgtccctgtg gtctgactgg ttggcgcaaa
    73141 ggagttgtgt tcttagccgg tgcatcaggc tctttgcgct ttgctaggtc acccattttc
    73201 atggacatta ttcttcttcc tccagactca acagataatc tacaaattct tcaaactcgg
    73261 cttccgcctt tttgtcgcgc tcgctaccgg tcatttcaaa gatagaacga ccagactctt
    73321 ccgcatcatc atagacgttg cggttataca gattgactgg cgcagactcg attccaaacg
    73381 tctcaacaat ctctttagcc gccagaatgc gagacacttg tgatggcaaa gccgggcact
    73441 ggttcatgac cgcgcggacc ttcactttat cgtttacatt gcgaacattg tcgataatag
    73501 gatcgatgtc acgtaaagat ttcaaatcac gacgcttagg acgcagcggg ataatgataa
    73561 cgtcagccat cagcatcgct tgtcgctgaa tttcggagtc gaagccacca gcatctacca
    73621 ctacatactc agctctaccc tgaagcgatt ttaggtgctt aatgatgtca tcctgaacgt
    73681 atgcaaaagg aatcagctca aggtcttcgt tctgtcgacg gtcttcacac cagcttgttg
    73741 tcgtgcgctg aatatctata tcagtgatat aaaccttctt cttctttttg actttaaggc
    73801 aaacggcaat ttgctgggca agggtggatt tgccaggccc gccctttgtg ccgccaacca
    73861 caaagatctt ggtcattgga gagttccctt tgcgtatata attatcgtct gaaacaactt
    73921 gttttcttat atgtgatata gcctaaatgc ctacggctgc ggtgtaaagg ttaaatggta
    73981 ggttgtgatt aacaattggc aatcatagct tgtatttgtc tgttgaaaaa agtaacaatg
    74041 acctctatat tacaagtacg gtgttatgcc gtacacaatt tgataatgca cacgtaatgg
    74101 gctatagcct tttaaagtaa tttttgagtc gaaaactcag tttgaacaga ggagattgat
    74161 ggccttccta catcacggcc atcaggagga atacatgtca gcactaaaaa agcagcgcat
    74221 cgatttgaga ttaaccgatg acgataaaag catcatcgag gaagctgccg caatgtctaa
    74281 ccagagcatt actcagttta tggttagcag tgcatctgaa cgtgccgtga aagttataga
    74341 gcaacaccgt agactagttc tgaatgaaga atcctggaat ctggttatgg acgctataag
    74401 caatcctccg gcaccgaacg acaagctgaa acgagctgct gatcgtctga aaagcatgga
    74461 gtagtttact cgtgagcaat acaacgatag agattttctc tggagagaaa gattatgatc
    74521 taaacggttt tgattgcggc gaagagtcac taaacgcctt tttagccaac cacttaaaaa
    74581 ggcagcatga gggaaaaatt cttcgtgctt atgtgctttg cactcaagaa gaaaggccaa
    74641 aagtgttagg atattacact ttgtcaggta gttgttttga aaaggagtcc ttaccttcaa
    74701 gaagcaaaca aaagaaggtt ccttatcgga atgttccaag tgttactttg ggtaggctgg
    74761 ctttggacaa gtcccttcaa ggtcaaggat ttggctcaat gcttgtaaca catgcaatgc
    74821 gcgttgtgta caatgcatct cttgctgtag gcattcatgg acttttcgtt gaggcgttaa
    74881 atgacaaagc caaggcgttt tataaaagtt taggctttat ccagctggtt ggtaacaacg
    74941 agcgttcttt gttctatcct acaaaatcta tcgaaaaatt gttcgaagaa taatgtgttc
    75001 ccctcatttg aggggaactt cgacatcacc aaccacaaac cctctctccc atcgtattgt
    75061 gaaaaaggat ttggcgttcg gtttcttcag tcattacatc attattgctg atatagatag
    75121 gctcagcccc atcgcagaac aacacgctgg ctgtttgtgg tttaatgacg cacccactta
    75181 tcatacaact cacgatgaac ggcagaagca tctttctgac gtattttact gctcgtctca
    75241 ttcaccactt tcactgtgtt ttgaagtcgt tttctgtctt cctgttttgc cttctcttcc
    75301 attgctcgtc gcgccgcatt tccgcccatc gtgtaagcgc cgacaagaac gaaaagaacg
    75361 gcagccagcg taatcaaagc aacttttagc tttgtcatca ggctgcctag catattagac
    75421 catccctttc tggtgtcttc ttacctgcga ccaggcaatg aatccggcca caaaaatagt
    75481 ggcaataccg aagatgatgc gtactgtatc cccgctagag atatgacctt gtgctttatc
    75541 catagcagcg gaaacctgcg gcataacatc ggcaagctgc gccagaccaa tacctgctgt
    75601 gacagttgcg cctgcggttt ctttagttac aggaacagcc ttcacggttt tcaccggctt
    75661 aacgacgcca gctcgacgca gaccttcttc aataacttct gccgcatacc aggtgttcag
    75721 cgtttttagt ggacctcgac cattctcgtg gcgaatgatt gcctcaacca aaggtcgaag
    75781 gatgtcgtaa tcatgcagat cgatgatcat gtctgcggtt acaccaacgg ctttagacac
    75841 ctcattaatg taggcgtcag tgttgttttc attcggcggt gcccaacgtt caataacttc
    75901 acgaatggta tcgatacttg agccgtcttt tgcgcgacgt ttgtcgtggt aggtaattag
    75961 agtcaccgcc agcgcacgaa tcccccaaac agggtcttta aacgtgcaaa agcgcggttc
    76021 gtctggattc gcaaccagac cttgccacgg tgatccttta tcaagattac cggggttatt
    76081 attacgaatg cctctcggag tcttcatcct tgatctcctg ttattgcagt ccatttttta
    76141 cgccatacgc ggctaacccc aaaagcagtg cagtaataat gaacgacgtt attttagaaa
    76201 caatgccgcc aaagaaccca ctggagatgg aatctaaccg gttaaggagt ttgtccaggt
    76261 tggagtgttg aatactatgt tgcgccgggg tcatatcacc aaagtaggtt ttcagctgat
    76321 cattgacctc ctggccaatt tcttcacgta gctctttacc taatttgcca acaacctccc
    76381 gagcaacgat cgcggcaata cgctcaactt gctctgttgt aacgcccgcc atctcgttcg
    76441 acatgttttc ctccatgaaa agtcaaatcg ggatggcgga tttatatcac atttcacctt
    76501 cttgttgtag gtgtgtactt acttatcatt caacattgca acggactgat gagaaagcac
    76561 ctactcctac ccatcgccag ttatagatgc tacccgcttt gtataaggta tagtcgttca
    76621 cttttttaac agcatatatg ggtatgactg tctcctgtcc accaatgata gcctcaccgt
    76681 aacatatagg tgtcggcaat ttctggcagc cagtaagaga gagaacaaca gctattgtta
    76741 gaaataatct tttcatcatt tactcatcag ttatacagat ttctatttat taccggtact
    76801 ctgccattta cgctaaaact atggccgcca ttaagttggt ttacgaatat cgagctaaca
    76861 gtatttccat atgccgcata tccataaacc attgtcatgg ctccgcccgg tactggaaca
    76921 gcgaacacat tcgcatatgt tgggattatc gctggtgggt aatcaaaaac atgccctcca
    76981 gagctgttcc actccgtatt attaagaaag gtaaatgaaa gtgggatatt tgccgtatta
    77041 tagatttcct ccccttgtgc attgaaaaaa ctcatgcccc aagtttcttt tttggctata
    77101 cctttcgaaa atacgtatat cgtggcacta gagtcgctac cctgacctcc gaaactgtaa
    77161 atcacctggt tgttacgcac tattctgttg gctattaatg aaacagcttt cgaataggca
    77221 acaaaaatca tcggtgaacg atctggagat atttgggtgt taaactctgt cccgccagac
    77281 aaagaaacaa cctgctttct ctgaaagacg ataggagaca aagaaggaga catccagacc
    77341 ttcccgtcag atcgatatat cttgcttccg tacattattt ttgaaaaacc aatatgttta
    77401 aattgccact agctccagac catgaaatgg tatttccaga aaccgtaaga cttgtgtatt
    77461 tcccattggt tacgtccatt atgtaatagt cgattcccaa tcccgcctca acctcatatg
    77521 ttctacttcc agaaccagag ggagtaaaat aatcgaggta ataaacaggg gccaacgcat
    77581 cgaccatctc ctgtcttgat ggcgaccata cctgtgcacc atagctcata aatatatcct
    77641 tatactttta agcaggggct ggtttagttc cagccccgat tgaagttgtt tattctttag
    77701 gtgaatttaa cttcgtttca agctcctcaa cccttttggt caattgctga atatgatgaa
    77761 tgagtggtac aaccaaacgc tcatacatca ccccttcagc gaccatacca tttgaggaaa
    77821 tatcttctgg agaatcatta tttgttggtt cacgccaatg gacatattgc ggggcaatct
    77881 caccaacctc ttcagcaata aggccgtaat acccccaatc ctttcggtca cctcgacaaa
    77941 ttgaacgata ccaaacaggt cttaatgaca aaagttcatc agcatatcga tcttgtaatg
    78001 tttcaatatc ctttttatag cgccttgaag atgttgaacg ccaaactgta ttgataccag
    78061 ggtttggatc aatgtacata ttggcaccgc tagtagttgt gccaatatcc catatagcaa
    78121 accctccact accgcctgat ttgacaacca gtgtttcagc aataatatcc aggttgccac
    78181 tatagcctgt cagtgatgat ctgcttgcat aggaacttag aaggtttgtt acctctgttt
    78241 tcgtataagc attaaggtta gccgcagtca aagtaatatc ggcagagcca tcaaacgcca
    78301 caccagcaat tttccttgct gtctgcaatt ttgtggctgt tgcggcatta ccagtagtat
    78361 tctgattacc ggttgtattc acaccaggga ttgaatcctt agcagtatat acctgcgccc
    78421 acgctgacca tgccgcatct gtggtatctc ttcgtgagcg aatgaaaact ggcgcatgtg
    78481 caccgctcgt accactccag ccaataagca actcgccctc accagcagca ctcgcacctt
    78541 tcatgtgcaa tacgttgcca tacgtggtcg ggtagctatt gttgtacgcc tcgtacattt
    78601 gaatgccagc agtgccttga gtagagccgc ttaacgccgt aactcggcca cgagatacca
    78661 atgtattaat gttgatatcg cctgagccat caaacttaac accattgatg tttctcgctg
    78721 tttgcaactt cgtagctgtt gaagcattac cgttcaaact accatttacg ccaccagtca
    78781 catttagtcc attaccaatt gatacagcac cgctggtatt attaattgta attggtcgca
    78841 aaccgttcca tgaacctaat gtgtctcctg atgctgttag catgaaatat gtgttcgatc
    78901 catcattacg gataaagaac ccaaagctac cgtaagcaat gcggaaacca tttgcatatt
    78961 ttgaaatgat ctcgccagaa gatgtcaaac ctccagataa tgtaccaccg gtaagaggta
    79021 atgcaccgac atcagaggct gttggtttgt ttttcgtgtt atatactctt cgccatcccg
    79081 gagaataatc tgtgccatta aatacataga taaactccgc attagtgaga gcaccagaga
    79141 cactcgtcgt tgtggccgtt gttatacgga tcgtataatt gtttgagcta ctaccgttat
    79201 taaatacctc tataacagct cctgccaatg gaataacgcc acaaccagtt tcactatttg
    79261 gtatggttgc actattggca taggcccaag cacatcgagc aatccatgac tttgtgttaa
    79321 atgctccatt attttgcaat aacgtcacta actgtgctgt tgttattgct ccgccattac
    79381 ttcctgtagt taaccaacca gtaggagatg ccgggcaacc tatatttgct ggcgacaagg
    79441 aaatatttgc cgagccgtca aacgacaccc cattgatagt acgcgcagtc tgcaacttcg
    79501 tagcagtagc cgcgttgccc gttgtattct gattaccagt cgtattaaca cctggcaaat
    79561 taatattcgc agtaccatca aagctcacgc cgccgatagt tcttgccgtc tgaagttttg
    79621 tagcagttgc agcattacca gtggtgttct gattacccgt agtatttaca cctggaaggt
    79681 tgatatttgc agaaccgtcg aaaccaactc caccgataga tcttgccgtc tgcaatttcg
    79741 ttgctgtact tgcattacca tttaatgttc cggtgatccc accagtaaca gacaacggac
    79801 ctgaaactgt tcctccggtt gttggcagtg ctccaatatc taacggcgtc ggtttctgat
    79861 gtgtgctata catcgtataa acaacaccat cggtaacgct ggaaggctta ctagctgaat
    79921 atgttggcga tgtataaata gaaactgtcg catttgcagt acaatcccaa tggatattta
    79981 cacgagtcgc ataattgcca atctcaacgt aaatatcata tgtatcgccg gatgtgttga
    80041 tccaggcgaa attcgttaat ccgacagctg tacgcttcca caaagcacct gtaatccctt
    80101 tggggtttcc attgcctgct cgtagaacca gttctgaaat gcctgcctga tgtggggagc
    80161 cgacgttgta accagcgcca ccaatcaatg cgatgtaaac gatggaactc gcttgtggca
    80221 ttgtaacagt agccagtttg aaccacccgg ccccgccaga gaaagacatc gttattgaat
    80281 ttaaagtacc aatatctttc ggcgttaatg tgatatccgc agtcagtgct ttcccgttaa
    80341 tttttcggtt agatggcacc ctgctgttcg cattgtcatt gactacttta actgcttttg
    80401 gcgttgcggc cagcgattca ctggtgctgt cgacagcact gctaagtttc acaacacctt
    80461 tagttgtaag gcttgcgtct tccatcgcaa ctgcaccggc aatctcttca gcacgatcag
    80521 cagcagcttc cgcacgggtc gcagcggatt cagcagcagt tttgctctga gatgctgccg
    80581 tcgcactgcc tgccgcctct gttgctttcg tggatgccgt cgtggcgctg cccttcgctg
    80641 ctgacgcctg tctggtcgcc tcatcttttg aagcagacgc agatgatgcc gatgacgctg
    80701 ccgaactggc ggacgatgcg gctgccgttt ttgaggattc tgcacgggtt tccgacgctt
    80761 tcgcgttcgt ttcggatgtc ttcgctgcgg aagcagacct cgctgctgcg ctggcctgtt
    80821 cagtggcttc gccagccttc gttgtggctg ttgaagcaga cgatgcggca ctttctgccg
    80881 attttccggc ggcggtggca ctggctgagg cctgcccggc acttgttgac gcggcactgg
    80941 cagacgacgc agccgctgtt tttgagcctg ccgcagccga ggcgctctgt cccgctgccg
    81001 tttcagaaga cctggcgttc gtctcggacg tttttgccgc cttcgcggaa tttcctgccg
    81061 ccgttgccga ggaagcggca ttactggcgc tcgaggctgc gctcgtttct gatgatttcg
    81121 ctgcctcttt tgaggccgcc gcatcccggg ctgaggtggc agcttctgac gccttcgtgg
    81181 tcgcggtgga tgcagaagtg gctgcagatt tttgtgacgc tgcagcattc gtttcagacg
    81241 ttttcgcggc accggcactt gtggccgccg cgctttttga ggactctgca gcggatgcac
    81301 tttgagacgc ttgagaggct ttttctccag cgatattggc gctttctgct gctgcggcag
    81361 cactggccgc cgcctcacga gctttgtcgc cagcagcatc aatcgcgtca gtgttatttt
    81421 tataccactc aacgttttcg ttgtgctcgt tgacgatctg cattagcggc ttaacggtca
    81481 cttctgtacc gtcttcacgc tcgattgtca cctcatccag agcagtcaac caactgcgca
    81541 tggacttgga atcagccgac atacgcgaca ttagtgctgt aaagcgcgcg ctaaactgtg
    81601 ttaagtcgcc ttcataggtc gtaatgattc ggcacggaac ttcagactga gtttcgccgg
    81661 tataaggttc tgagagaaca atgttcgtat cactggttac gcgcttgatc tcatacagct
    81721 tattgtcggg gccaatgacg atcatccccg gcaacacacc attagctgtt acgttccagg
    81781 ctgtccctgc cccaaccaga gtattgctgc cctgtgtaaa tgtgatagta ccttccctgt
    81841 accacatgtt gaatatgctc cttgatttgg tgggctatcc ttgcccacca ctaagtatgt
    81901 acctacttat ttttaccgat ataaatattt ttttctacat tacagaaggc caattcttac
    81961 tcgtaggacg ttgttatcgt cataaacgtc aatccgctga ccatttatga ccagtcgccc
    82021 attacccccg ctattaccgt tgatctcaag cgttccattt ttgccgaacc gccatccaga
    82081 tctaccgcta acaaaattgg tagattgcag atcgcctact tttgcattgg tgattgtgcc
    82141 atccttgata tacgctccat tcatataggc gatactgttt tcgataacaa atggcgttgt
    82201 gatcttcccg ttaacagagt tgaccaaacc aaacctgtcc gcctgaacca aaaactgaga
    82261 tagcccagtg gtgtcaatac caagcgcaat accggcaaca tacttctgcc ctccgctcgt
    82321 tgaagtttcc attttcaacg tccacgcggt tgacactttt ttgttggtat cagcaatagc
    82381 tgttgcctgc tgttgaattg tcgcggtatt tccatccacc tctgctttca gagtatcgat
    82441 tcgcccactc agagcattat ctgcctgcgt tctcgctgtc gtttcagttg ttaccgccgc
    82501 ggaaatgttg gctgccgttt gagactctaa gtttgtgatt tgagtcgcca atgcagcatc
    82561 ttgctctgta cgcgttttcg tttcggttgc tacagccgct ttaatatcct ctttgtattg
    82621 agaggtcagc ttggtgatct gagacgacaa cgccgagtca gcatcagttc gagcctgcgt
    82681 ttcaactgca acggccgcac taatatcttt cgctgtctgt gcttttaaac ttgaaacctc
    82741 ttttgttaaa gcggtatcac catctgcacg agccgttgtt tctctggcaa gagacgcctc
    82801 gagatcatta gcttttgctg taagagacgt aatctgggta gacaatgcac tatcggcatc
    82861 agtccttgct tttgtctcta cagcaaccgc tgcggcaata tcagttccgg tttgtgctcg
    82921 caggctgtta atttctcgtg agagcgcctg gtcagcactt gctctggcct cctgctcctg
    82981 agtgatggcc gcggatatat caccgtcaac ctttgactga agctggttta tttgtttggc
    83041 taacgcagag tccccgcttg ctcgggcctc ctgctcacta cgtattgcag cagaaatatc
    83101 atcatcaacc tttgcctgaa gttgggtgat ctggcttgcc agagccgaat cttccgttgc
    83161 tcgggcttct tgctcttcct taatagctgc gacgatatcg ttgcttactt tcgactcaag
    83221 ctgagtgatc tgtgtcgtca gagcttcatc ggcagatgtg cgagcctcct gctctgtact
    83281 aatcgccgcg ctgatatctc cttcaaactt agattgcagc tgagtgacac gctttgccaa
    83341 cgcttcatcg ccatcggcac gagcggtgga ctcttccaga atactggcct taatgtcttc
    83401 gccaatttct acgcgaattt cctcaacctt cgtggccatt gcagacatat catcagcaaa
    83461 ggttttctgt gttgttgcga tcttcgcgtt attgaccatc tgcttgtgct ggtcttcatc
    83521 ttgacgaaga gccaggtcaa tatttgtttt ggctaacgcc tcaatgttcg tagtcagttc
    83581 tgcactggca cgatcgacct ctgcaaccgt ctttttcatt tcttcaacgg cagcggagct
    83641 ttcctctacc gttgactgca acacttccag ttgtttagcg tttgcagcat cgccttcaac
    83701 acgagcctcg ctttccttag caataagagc cgccgcttca tctcttgcag cctttattgc
    83761 ctcgactgta ttagcgagag ctttatcgtg ttcagatacg gtgttttcga tttcaacaat
    83821 tgcagcatca gtagcatcaa ttttttcaaa cgcttcattg accttgtcga tcgttgccga
    83881 aacctcacct ttaagctcgg tttgcgcgct ctccaaagca tcgctacgct cgttgaattt
    83941 tatttcaaaa tccgcgaggt tatcgctgaa ctgcttatct aattcagcta tatcttcctt
    84001 aacgtcgttt accgccccct ccaaagattc gacgctctgg ttgatatgct catttaactc
    84061 gtcaactgct tcttgagagg ctttgctgtt gatgtcctca agcagagcct gaccaagctc
    84121 ggaagatgta attttgccag ccaggaacga cagtacatcg cgagttgtcg cctctgtacc
    84181 caagtttgag ttcggagggc ttaacatacc tcgcttgttc gatgctcgaa cccagtaata
    84241 ccacgtttcg ctatccccaa gaccagcatg tgtaaaggtg gtgcttgcag actctgcgat
    84301 cagtttcgcc gtatccagat tgttggtctg ggatgcgtaa acattaatgt gatcaaggtc
    84361 taccgaatct ggattaaccc aattcagtat cacattacga tagtctccaa cggccgttaa
    84421 tgacgttggg gcatccggcg gtgtcattgt gcccaacacc tgataaacgg tactgataat
    84481 ctctgttttt ttaccgttga atgaaaccgc atacagttgg aagtcgtagt gtccattctc
    84541 cgcgacatta acgatttcgt attgctcttc ggttacacgc gccgattgcc agttcgatac
    84601 attgttttca tcagaacgtc gccaactgat ccaatactct ggagattttc cttcccatgt
    84661 tgcagtcagt tttactgaca ggttgcccgg gcttgagaga taagtccctt cagtgatttg
    84721 cagattagat ggcttggagt aagttgggtc caataccgtc gtattctgcg ggatcagcgt
    84781 tgcgccattg tcgattgcct catatttaga cggattgttc tcaacagcgg tgatgtcaaa
    84841 gctacccggc gtttccccct gcgcgatgtt aacgatgcga acgcgcatag gttcgagatc
    84901 tggttctgta attgtccaga caccgttcaa aacaggcgtt tccgctgacg acagggcttt
    84961 tgaaaacgta acctttgtta tgttttcgcc agtttcaaga acatcgcgtt caacgatttt
    85021 gccttcctga ttcaatatcc gaataaagct gccactttta gctaacgaca caggcgcatc
    85081 gagagtgatg ctgtttttgg taaactccac aattcgaccg gagttacgtt tacctgcacg
    85141 atatttgttc tgaatcagaa cggtttcacc aggcatcaga aatgaggcgt ctaagccggc
    85201 agtaaatgta attacatccg actccattct ggcggtatac aaaagccaca aaccaactcg
    85261 gtgagcctgg cctcggcttg tacatccaaa tgctacgact tctgttttac gctcaccata
    85321 acggcgcatt gcgtcctgat cttcaacgta ttcgatgttt tgcttataac cgtcctcctt
    85381 gttgttgtag gttacgagcg caacggatgg gcgatcttta cgcgcagaac ctttataggt
    85441 aaacagtcca tctttgacgt tggagttggt aaacatcatt accggatctg atgggctatc
    85501 ctgcatgatg ttaaccatgc caccagccca aaacaccatg ccgcggaatg caccggcaat
    85561 atcctgaatt aatcggtatg cgtcttgtcg actggtgatc tgcgtattga ttgcaaagcg
    85621 tttctcttta cccccaaagc catcatcgac ctcttcgtca caatatcgac caatctggta
    85681 gagctggccg aggtcaatca tggattccga cacaaattgc ccaagaccat accgagtatt
    85741 ggtaagcaaa tcgtagagaa tccacgcagg gtttgaagaa gacaacagct taaaagtacc
    85801 gtcccatacg ccgacataag tattggtgta ttcgttgtag tttgacggta ctcggatttt
    85861 gataccgcgc accaaatacg aacgggaagg cattgtgcta ccaaactgct ctgaattgac
    85921 cttcaagcca acaagagcag agttagggta gttcattggc gtgtcgacaa tctcaccgat
    85981 ggaatcaacc catgtgtcgt tgtagaggta ctggctacta ttatcatctg taagacgaat
    86041 caccctaacc ttgtatgcac gaccaggctt aggcagtttc agctcatagc tacggtaata
    86101 cacgccggtt ttctttgccg ttagcgtgat atcaacgctt ttctcacctt ctgccactac
    86161 atcagaaaat gttgcgtctg cgttggcgat ctggaatttg tacttcaccg tagtgccatt
    86221 tgtatcgcca gagcttttat caacgctacg taaagagggg aatttcatga tgacacgaac
    86281 gcgatcagct tcatcgttat cgattgaaac cgtaacgtcg tgtgtttttt taagctgaat
    86341 atttacggac ttaggcgttt cgacaaaatc aaaaccagcc attggcgtct gatcttgtga
    86401 gccgtcacga aaatcccatg taataccgct gaagttggaa gagccatctt cattcaagat
    86461 cggtaaatcg tcgatgaaga tcgactttgc gccatttacc aggccaccga taaccccctc
    86521 gccaagaagg tcgaggatgg ctgccatagc acgagaattg acagtatcat cagcttcaac
    86581 cggggtacgg ctggagcttt tacttttctt gccaccagca ccggcaataa gaagcggtaa
    86641 ttttttcttc ttgaactgtt ccatgtccaa aaaatccttg attacataag ctggtcgatg
    86701 gtgatagaag aactcacgac ctgtgagcca actaaaattt cctcgccata gataagctgt
    86761 acagggttcc cctggtttgt tgtgttttga ggtccatcaa agtaataaga gttcgagtta
    86821 tccgcctgcc tcacactttc gttagtggct tgcggagata tgatttgtgc tataccgccc
    86881 atcatcagtg acaaaccgag aggcgcaaga gcgggcaccc atatcgaggc aaccattaat
    86941 actgctccaa caaccgtctg aaaccatcca aaagcagatc caccacttcc tcgcggaaca
    87001 ggggtaatgc ggatttttgc aatattgtca gactgcccca tcatctgata ttcactctcg
    87061 tccacagacc acttgtggcc ctgtttattg gtgatctgga tgtggtatct gtcataggtt
    87121 ttgatattgc gcttcatcca tgctttaaac ccaggcttgt tggcctcaat taaatccaga
    87181 gcctgttttg tattacgcac ctttagatgc cagtggcggc caaaatgttt ggccataggg
    87241 ccgccaagct gcacatgaac taactcagac acgtctcatc tcccttgagc aagtctctgt
    87301 gacgcaagtg atgcgtcgta tgtttctgat acatcccgcc gtaataagca cgacaactta
    87361 ggcggtcgat ctggtgatga agaatcattc catcgccgat ataaaccgcg cagtggtcag
    87421 gcattttccc gtattggata aagaagatgt ccccacgttg aggctctgtt ccgggcgcaa
    87481 gacgcaccaa tccctcgtta cggtagttct gatcgagaat gtcgttatcg ccggtgtacc
    87541 acgatggaat atgcaggtgt gcgttcgggt ttagttcgac gttaaactca cgcttcaaat
    87601 agtcccgaca caacatccag caatcgaata cgccaaatac atacggtctg cccaggtatg
    87661 gcatttcgaa accatcaggt gtgatcacat tcatctcgct aaaatggaaa ggggcatctc
    87721 cctcaacatt cttgcgaata gccagaatca tccacggaac ttccgtcgct tcgcagcctg
    87781 cacgatcggc atcagatgct tctgctgatt catcagtatg tgaatgccag attgcgataa
    87841 catcacccgc atcctctgcc gccataatgt cgtcaacgtg cattacaaaa gtgttctgcg
    87901 ggttctccga aacattccgc gcttccataa agcgatattt gtcgccatta gttctaacca
    87961 gaaagccaca cgcttcatta gggtagcgat ttatggcgca gagatagatt tgctgcataa
    88021 cgtcagagcc aagctcaggg attgctttat tacccatacc gcgtagctcc aataaatccg
    88081 ccaaaatgga tcacaccgtc ggcaaaataa ttccgacgcg cattacaggc gtcataacgt
    88141 tttgtgcagt aatccgcacc agacatagac gtctgctggt tatttttgtc gaaatatgga
    88201 ccggtatagc cgcattctgg ccctcggtat ttccacgggc aggtgttttt aatgatctga
    88261 cgatacggca gttgcacccc catcaaatcg aacacactgg acaattcaaa ctcgacaacc
    88321 tgacgagttt cgagagtttt ctgttcgata aaccacattt catccgggaa atgttggttt
    88381 ggatctgctg ttgggttgcc gtctttaaaa ttaacggcat cgaggaagcg agccagcgtc
    88441 atcttgcgaa taatgcggca gccaacaaga tcgtcgttcg cctgaacttc cgcagagacg
    88501 gttccggcaa agttcgatac ctgaattttt ggacgtggca acgttccctg gccagttttg
    88561 tcaaagcctg atgctttgat tggccacggc tcgtatgtca ctccttgcca gacgaccggt
    88621 tgcatcagtt cgtttgttcc ggcgtggaag aatagcttcc cccctgaagt tgtgttcgac
    88681 atatccaata cgaacaactc aatgagtgca gagggagata agctctgaat atcagcttta
    88741 attcccattg tttcatcctt gaaataagta ggcgctaaca tcctgtcagc gccacaatga
    88801 tagtaaatta gtacttactt atccagatgc ttaagcctca aatacttgtc tgaatgtagc
    88861 agttaagaca cagtacccct gatatcgctt gaccgtatga ctgtcacata caactacaat
    88921 ctgcttgcct cttggattaa cccaatagaa cgattcaacg cctgatcgct cagtcaggaa
    88981 gtcatcgatt gcattaattt cgttgtatga tctggtaaag gttaacgacc attcttcttt
    89041 aatacgatta agaccttgag cctgtcgctg ctcgtagtca tcaccaaaat taagtaccgt
    89101 tacattcggt tttacgcttt tttcagattc gtaatctgga taccaattaa acgtttgtct
    89161 ttccatctca catccttgtg agactgcccc ggtcggggca gtcgatagtt agttacgttg
    89221 agtgtttggg ttgagtgatc cgccagggcg tttctcttga gcgatagtct caagcgcgat
    89281 tgctttcatc cgttgagcgg cattgttcca tatgctttct gtatcgccgg attctgtcgt
    89341 gctaccgtca ctatggacgt tgatctcaat tgatacagga gaaagaacat ttccgcctcc
    89401 actcatacca tcggtactga gcgttacagg gattgttcgg ccatcaggca atggaacata
    89461 cgcctcattc atagaacctt ccccaaacaa cgctaattga ggcgagttag caataccgcc
    89521 tttctggtat gcccggagcg ggatcacgcc gtcttttccg aatattcctc catttgcaaa
    89581 cttcgggatt gcaggaatgc ctttagttcc gtcagccaca ctgctggttg cggttttagc
    89641 agagcttgat gtcacgctgt cgaaaccacc gccagcccaa gctgaaacaa gaccagatgc
    89701 aaccgttgct ccaaaactca accacttatt accagagccg gaagcattag ctccaagcat
    89761 tgcaaacgcg gcagacagcg cgccggtaac agagctgagg ttctgcatcg agaagatgga
    89821 gtccttcacg gcttttgtct cggcatcttt ggcttcagtg ctatcaaata gccctgatac
    89881 ccagctacca atcgcatttg ttgcagtgcc aattgcgctg gtggtctgct gtgttgtttg
    89941 ccccaatcca gttaccgaac tggacgtctc cttcgtggct tctcctaccg acttgtcgcc
    90001 gttaacagtg ttgcccattc gtaccccttg attggcaacg gcggaagcaa ccccggtcag
    90061 caaattacca ctctgtgaac taccagctgc ggtggttccc atccccaaca tgttcattag
    90121 aggcagcgtg atctgcgact tcacgaccat attggtgata tctttcaaaa tggactgaga
    90181 taggctggag aagctcatct tcccgttaat aacgaaatca gtcaggacat cagttaagcc
    90241 actaaacaaa tcagtccagg tgctttcgat ctgctctgcc aggttttcgt attccagtgc
    90301 caacttctgc gtcgcagtcc ccgtctcttt aataagcgcg gtattgccag cagcaatcag
    90361 ttgattgatt ttctttgtat aaagcgccac aactttagga tcagacgcct tatcacgaag
    90421 ttctatcaac gctttgagat tgcggttgta ggtgtcttcg aaatcagcaa ctttctcttc
    90481 gcgagacggc gtatagccag cactaataat ggaatccgcc tccggtgccc aagtggagat
    90541 catctgctca acattgcggc gattaaacat ctcgcgatat tcaggtgtcg catttttgag
    90601 gtcttcaaga cgttttttcg ccttgtcgat catctcttga gtgatgaact cgttaggaac
    90661 cgcattagcc aaatctgtca gcgatttcgt tgtatcgcga agagactgat caaacgatac
    90721 cgtagcctta gagctttcac ccatttgccc cataagctga tcggctttgt ccagagcctt
    90781 ctggtatccg gctgccagtt tctgttgcgc tgcctgttcc ttcttggccg cacgctgcga
    90841 ggcgttagct gatcgttggg ctgctttctc ggcagctgct gcatcctgtt cacgagcttt
    90901 agttagtgca gcaatggctg cggcacgctc ttcatcgctc attttctcca gagagctggc
    90961 gctggatgct ttctgcaaat taagctgcgt cttgagttgt ttaggcccaa taatcggctt
    91021 accttcgaag tccatcatcg gagtgccgtc aggcaaagta cgttgataag tcgcagaatc
    91081 catctggttt cgcatatatt gcgccagcgc cttctgagcg gctttatcag ttgtacctaa
    91141 cccaagaaca gtcccctggt ttgacattac gcccttacca gttttggccg cgttatctct
    91201 ctcgaactct gcctgagtca gctcctgagc aacggcttcc aaatgctcct gataaccacg
    91261 gatactgcct tgcagtttct ggatttgctc ggtatttcca tcctttttgg ctttttcaag
    91321 ctgatcatta agagtcgcta tttgcttctc ggtggcattc ttacgagaag aaagtgaatc
    91381 aaccagtttt tgcgcaggct ccagatagct tttgtttacc gtttcacgta acggtgccaa
    91441 tagcttgttc ttttcgtcat ctgaaagtga accgtcatca ttgattttct ggatcttgtc
    91501 cagagcctcc tgccgggctt tcacgaatgt tgccgcaaaa atctggtttt ccgctcgaat
    91561 tttctcaatc tgagattcgg cagcctcttt agccaaacgc tttgctacag cgccgtcacc
    91621 aagagctatc gtgccggtta tcttttgata ctcttcctga ttttttttca ggcgtgcttc
    91681 gatgtcagcc ttcgactctt tgtgagtaat aacaccggca gagttagata cgtaattaac
    91741 accctcacca gtttttaatg ctcgttgatc agcaagaatc tgcttttcga gcttttctgc
    91801 gcggtcggcc atttgtgcac gtttggccgc cgtcatcgcc tctggtattt tcctaatctc
    91861 gtcaacgacc tttgaagttt cgctgcggag catggtcatg tacgtgatta ggccagcaac
    91921 agctacagtg gcaactgtaa atgctgcccc tatagggttt gctgcaatga acgccgttaa
    91981 tccagcaaaa gcgcctttaa gccccgtaat cgccccacgg atggcgaaaa taagagaggg
    92041 gatcggagcc agccccatac gtgccgcacg attgaatcgg gttactgctg tagcgccgag
    92101 gttaaatgga gtctgtatgg ctgtcgccat cgtggcaaag gtgctaacca tctggctgcc
    92161 tgcgccaact acccccatga tccctgctcg catcagtttg aacgcaacca tcgcggccac
    92221 gaccttaccg agattaatta ccagctcttg gttctttgct aaccattgag caagctgacg
    92281 caacccatcg attgccgtcg ttaaccccga gcctaaagaa ttggcaaacg aaatcccttc
    92341 ggcgctattc atgattgaag ccagttcttt catccccttc gatagagaat ccagatatcc
    92401 ggcctgacca acccgatcag caaataacgt gaatgacgtc tgaagttgcg ccagcgcacc
    92461 tgtgtaggtt tgcatcatgt ctttcgctgc gttctcattc tccgcacgca gaccaacaaa
    92521 catcagagac aacgcctgtt ttgcctcaac cgtaccgctg gcaacggctt tagtcagttc
    92581 ccccatagtg atgcctgcgg cgtctgccat tgcctgcatc gcgttaggaa cggcttcacc
    92641 taattgctga cgtagctctt ccattgacac aacgccctta ccggacatct gctgaacggc
    92701 cacagccgct cgtttcaaca gctcactatc accaccaaaa cgagcaacgg agtccaccag
    92761 cgccttcaga gaaccatcgg ttggatctaa gccagcagaa cgaaacttca cgaaggagtc
    92821 tgttaacgcc tgcatcgcga acggcgcatt ttgagccatg tctacgatgt acttcatatc
    92881 atcagcggca gcctggcccg ggttggactt ctccttattc aaccctcgaa gcatcacccg
    92941 catacgttcc atttcggccg cagcttcaac aataggcttc tgccacccaa acatgatgtc
    93001 agtaaccgtt ctggctgcat ctccgatctc gccaagcagg aaaatgttgc cacgaaggcc
    93061 agagaacata cctccttcgt tacttttacc gctatggcca gaagcgccgc tacgccgccc
    93121 gctaccacca tcgccacttc cagatgtacg aacgcgtacc ggcttgctaa tcagttgctg
    93181 acgtccgata acttcgtcca tctgctcacg aacctttttc agtccctcgg cagcctgact
    93241 cgttgtgaca ccccaattac tgagtcgctt cgtcgtggta ttaaggcgcg tattcatgcc
    93301 actcacggac gcagaggctt ctttgacctc cgtaccaaag cggcttgcgc ttttgcttgc
    93361 ataggtcgcc caatcagaga aatcatttag ctctgattgc actttacgta atgacgcggt
    93421 gagtttatct actgaagaag ttgtcgtatc gacgcgctca atcagggctt taagaccaga
    93481 attgaggctg gtgatgttgc cacgcatttt acgcgtagca tctgaagcaa gctcaaaacc
    93541 ggcagctaca tcctgtagtt tatctgccgt agaatcgagc ttgctttcca gaacgccaat
    93601 gatacgggcg accgaaccca aagagcgttc aaaggtttgg attttttgag caggctttgt
    93661 tacctgctca ccaaatcgag taagcagttt ccccgcacga tcgattgacg ctgtaaactg
    93721 tttgtcttcc agcgacagga taaactctac gttttgtgac attcccttgt catcctctgc
    93781 caaatatttg catcagttgc tctttggcgt cagggtctgc cttatcctgg cttggatcgt
    93841 agactttatc tgttacgact ggtcttccaa tcctgagttg caaaccctcc atgaacgcct
    93901 tcacagcctc gccatccgcc tgggacgcac gagcgacttg taagttgcgg acatcctctt
    93961 ccgcacgcag acggtctata ttgcgactga gcatccagaa catcgtgaga ggaacgttca
    94021 gtagctctaa tggcgacacg gcgtagtgag caactacacg actgaaatag aatccgagat
    94081 ctattgagac ggtccttgtc ccggattcat cgcgggaaat tactttgccc cttcgccagc
    94141 cgctttttcg ttttcttcat caatcacttc catagcgaag gtgaagatct gctggagttg
    94201 cggaacagtc agtttttcaa gaacttcgtc aggtactgaa gggataacct tacgaaccag
    94261 atctgcataa gctgtcactt gctcaacagg agacatgttc atgagatctt tgccttccat
    94321 ctgcttgatg gaaacgaaca gacctaccgt catttcaacg atgggatatt cctgaccgcc
    94381 aaatttgatg cttttcttcg gaggcagaat ggaatctaga tcgagtaatt tggtcattgg
    94441 ttaaaatcct tttaaaagag aggcccatcc tgagcctctg cttaattact gattaatctg
    94501 cggggttaat cgttactgat ttagttgcct tcttaccacc gctattgctg gtgaaggaga
    94561 tatttgtaga accctgcgcc acaccacgta ccagacccgt ttgatctacc gtggcctttt
    94621 cctggtcttc ggattcccaa acaccggttt tgtctgcggc atcagctgga gtgatttcgg
    94681 ctgtcagttg cacagtttct ccagctttta cggttggaga ttccggtgct atcgacacag
    94741 tttttaccgg tttagggccg ctcattcttc caagaacgcc ttcgtcatca gggtacgcac
    94801 tgaactgaac agagaacaca cgaacatcat cagactggta ggtcatagtg aagttgcccg
    94861 cggttgccgc tttcgggatg gtcaacacat agtcggtggt gtcctgcgga gtcagaacca
    94921 gctccttcgc cacatcaatc aggttaacgc cctgtgcaga tgtgatcgtg acagagttgt
    94981 catcttcgct cagagtagaa ccaggcatca ggtcaaccat gttttggagt acagactcag
    95041 ccagaggcgc ggtgatggta atgttgcgcc cctgaactaa ttcggaaatt acggtctgcc
    95101 ccaactgatc gacggtgact ttcagcgttt cagtggctac ttcaacctga acaccacctt
    95161 tggtgtaacc caaatccaca ccaccaaacg acaccttaca ggcaccaagt ttgatgtttt
    95221 ttacatgggt attagacatt gatggaaaac tcctttttcc gttaattcta cgcattcatt
    95281 gcgctaatag taagtatata cttaccaatt gagttaattc aataaatagc cagcaaattc
    95341 aacaggaatg cctgcttcta ttaatgcccc atcatttttg ggataaatga ttggcatcgc
    95401 catcggtcgt acaagtcgaa aatacacacc accagattcc gtttcctcta ctggaaacat
    95461 ctcaatgatt ttattggctt tctcaaccgt cgtagtaatt gacgaaccac gcacaatgat
    95521 tgtgaatgat tcgtgataaa agccctgtag ctcatgatcg atgctgatgc cggtatttgg
    95581 gttaataagc aggacgccag atttcacatt ggcaggcaag tagtgacaga aaatgtcagc
    95641 cccgaccgtg ccaatctttg ccttctgcat caaacttgca aacgcttcga taaacacatt
    95701 aacctctcgt aaaaccggct tttctggcag cctcaagaat cgcttctgag aactgcttct
    95761 cgctaatttc caccgctctt tccagaaagt gcggcccaac acgaggttta acaccggcaa
    95821 ttggtgggtt tgtcacgttc ttcattcgag aaagataacc gagtcggtat ttacccagct
    95881 ccatgtactt agcatagtca ccgacttcta cgcccggatg cccctgacgt ggttttgctc
    95941 cagacacaga aagctcaatg cgcagccctg aataaccttc tttaatcacc ctggcaaaga
    96001 tggctgtctc cagagatccg gtttccagcg gggccattgc acggctgagg cgctcaacca
    96061 aacgcgccag cttttccatg tcccgaataa gatatcgctt aaatgctttc tggctgttgt
    96121 tgagtctatt ccccgcacgt ttgaactgat gcgcatcgta tttcagaccc atatattcgc
    96181 ccctacttca agatgcccag gtcttcctcg tagcccccag cggcgatgaa cactggacac
    96241 ctttaatttt tgaccttcaa ggatcagtac atcatcaagt tgtacagccg cttctaaagg
    96301 gacaactaac acagcatcaa acagctccag actcgcctta ccacgactac cagagctatc
    96361 agcacgaact gacgatttct cattactctg ttcgaactta accacgccga cattcgtctt
    96421 cctgacgaat tgtaattgcg cctcaccgta aacgttcttt gcgccaaagc ggtagagcgc
    96481 aatttctgtt tgccatgaaa tattcatgct ctctccctgt tgttgtcggt cgctctcatt
    96541 actggccaaa aaccctttag accaaaagta aataatgcga ctggcgttac gcacggcgaa
    96601 caatcatacg gttgttgatg taactgacca gcaagcgcca ggtactacga gccacatgca
    96661 cgtttgcagc tttaccggta cggtacatgt tggttgtttc accgatggac tctgacaaaa
    96721 tgccatcctc tcgtgctgcg gctacatcat tgccatttgc gatctcacac gcttcgttga
    96781 caacggcaag catcaacgct tctttgaagt agtcagggaa ctcttcaaac ttctcctgcg
    96841 tcatcttttc ccaatcgact aaatcatgcc ggtacgctcc atctgctccc cacggaatat
    96901 catacacatt cagcatattt tgagggcgat cgtatcggtc aaagtcgata cgtagaattt
    96961 tgcggattga gaacggtaaa gttttaattc gtctggtagc ctcaatgaga cgcttgcgca
    97021 ttaagccttc accatccgac agcaaagtgt ccccattcag catatcgatc gcctgcattt
    97081 gagcatcagc gacagttgca aacgactgtt ctggtatcga cagttcaaaa ctattcagca
    97141 gaacatacat ttgccgctct tcatgcgtca gacccgatgc aacagccttc acaatgacgt
    97201 accgcagatc tcgctctttc tcagagagct ggttatattc agccgacacg acaaccggaa
    97261 tcgacatttg accgttggta atttctagcg gctcgccatc aacgagaata gccccggtgc
    97321 tgtcctttac tgtgtaggtg gcagattcga tatccagcac gttgaaggca aatgaaagag
    97381 aaacagcttc accgctacga tacgagtcga tctgcgccat tactcaccac cttgtgcttt
    97441 caggatgccg tcaatcattt cgacaattcc tttcgctttg acaccaacct gattaccaat
    97501 aactcgtaga ccggcaatac cttcgttgtc tgcaattgac tccagttctt ctcgtgtgaa
    97561 agtctggatc tgtttggccg gttcatctgg tgtcccacgt ttcattggca caatgtcagg
    97621 cgctgctggc tcggtaatca ggtctgctgt caattcacca cgatcactgt atgcggcaga
    97681 cggagaaaca tttttgccct ctactgttga cgctcgcatt gaagcacaaa tcctctgctg
    97741 atcgataaaa ggcaactccg ctacggatac cccgttctcg aactgaacgc cacacagcat
    97801 tcccgaataa ccggaaaatt gcggttctaa taaaacaatt tttgctggtt tcataacgct
    97861 ttctctccac ttgggcggcg aaagccgccc aatatcggtt attcctgtgc ggcagtaact
    97921 tctacagttg ctgtcgcttt gtggctacca tcttgggtag taacctcgat tgtggcagta
    97981 ccagccgcaa caccagttac aacaccggtt tcgctgtcaa ccgttgcaaa ctcggtattt
    98041 ttggattccc aagtaacggt tttatttgtg gctcctgccg gctcgacatt tgcggtcagc
    98101 tgaacagttt tgtttgcctc aacggttgtg ctatttgggt cgatttcgac gccggtaaca
    98161 gccacaacag gcgcagttac ttccaccgtt gcagttgcct ttttgctgcc atcttgggaa
    98221 acgatctcaa tcgtcgcagt gccaacttct acaccagtaa cgttcccgct ctggtctacc
    98281 gttgccttat cttcattttt tgaagaccag gtaacagcct tattcgtcgc atttgacggc
    98341 tgaacattcg ctttcagact gactgatttc cctttctcaa cagaggttga gtctggcgtt
    98401 accgttacgg attgaacggc taccggattt acggtgactt ccactgacgc ggaaagctgg
    98461 gtttcctgat cagttgcagt aatttttact ttgcctggtg ctacaccagt tactaatcca
    98521 gtgccgttaa cggttgcaat ttgatcattc gctgacttcc aggtgaatga actggcactc
    98581 ttacccatac taatacctgc actaagttga acagttttcc caaccaaaac tgacggtgaa
    98641 gccggggtta tgtttacgga ttgggagagg ggaaccgcct gcaaacaagc agatagctga
    98701 ctttgttgcc gctcggttaa aggttcatcg gagatagaat tggtaaatcc ggcgcggcac
    98761 atatgccccg taaaatccga aaatgcctct tccgtaatct tcatcttttg ttctggcatt
    98821 tctcgctcct acaaaaaggg tgggcgtata gcccaccctt aaacatagat aattgcttat
    98881 ctatcacact gattaaattt ttacattggt cagtgcagcg atagccttat cgtgcttatt
    98941 cgccagagag cagtaccact tcacacgggt acgtactgcg tctttgttct gtacagtacc
    99001 aatgttttca acaacgatac ctgcgttatc gccgccatac agaccagtaa cgccgttctc
    99061 ttctgacaga tgcaggcagt agatgcttgc tttgccagca tcggtcggga tgaagtcgtt
    99121 gatgatgaac ggaacgccat tatgacacag catcggacga ccgaagttct ccatcatgat
    99181 ttcagacgga cctacgttta ctgtacgcag cagcgcacgg taagcacgca ggtgctctga
    99241 acgcatcatg atgcagtcag caccaagatc tttcaccgca tcgaccagtt cgtcgaacat
    99301 agagaaagtc atagatgcac cggcgatatc gatcttctga tctgcgtgca tcaggcgtgg
    99361 aatgccgtca aacgccttgt tgttggagct ggagtcaccc aaaatcaggt tgcgacggaa
    99421 cgcacgagcc agacctttaa ctttctgacg aacctgaata gccaactggt tgttggtgtc
    99481 ggccatagtg gtcgccagga atttatcaac gtctacgtca ccagccagaa tacgcagttt
    99541 cgcaacgcat tcttcgaagg ttgctgcacc ttcagggatg gtgtcgttaa cgtcgatgaa
    99601 ggtagcttca ctcagcgttt tttcacggtt gtacagatat gcctttgaat taattttcat
    99661 aaaaggcagg atggcaaaca ggtcatcgcg atcgatgata gtttcgatca caccctgttc
    99721 aagttcgttg ttagacagct tttcagcttc ttcacgcagt aatggcatct atcaattccc
    99781 tttgatttag atgttactta agtccaattt tccccagacc ggaggccaac ttatccatag
    99841 tcgacttgtt cttcggttgg gatattgtgt aggtcgattt ggaatgtgag cctacaccct
    99901 gcttggcttc gctacgcatc aatgcgtcag cttccggatc tgcccgcaca atgcgttcaa
    99961 tcgcggattc aaacggcaac ggcttacctt caccgtcaac cagaacagct cgttctttgt
   100021 gacctgccgg tttgtcataa cccactacgc taccgtcttc acccacttca aaatgagaac
   100081 cgtagatcac gcgggctttt gccggggtca tcagaacttt gtcacgtagg aagagagagt
   100141 tactgaagga agcgcccacg gtcatctcga ctaattgaga tttcagtgat gcgttttcac
   100201 tttccagtgc tgcataacat tcgtcacgct gtgccagctc tgcctggtgt gcttcgatca
   100261 tctgtttttt aacagcatcg aactcaccac gacgctccag ttcagcttgc tccgcctcac
   100321 gacgtgcgtt ttctgcggcc tgttcagctt ctaaaagctg gcgagcacga gccggatcga
   100381 tatcaccgta ctgagcaagc tgatcggcca atgtgcgctc tttctctttg cgcttcatgt
   100441 tctccttcag cagttcagca ccagctttct tggttttacg aagttcggcc agtaactctt
   100501 cctgagtcat gccagcgtat tcgtcatcgc ccttcggctg ctctttttgt tcaccctgtt
   100561 tatcaggatc ttgtgtactc tgctcattat cagcagctac accgccagcg cctccacgct
   100621 catgcgcttc agcgacatcc atcagaccac gacgggccaa aagcatttgc cacagattca
   100681 taaaaattcc ttttgttact tatcactcgt tctcttgagt agatgagtcc ccattccctc
   100741 ggggttgatc ttgcccgctt tcttgggctg caccacgatc ataagtaagt actgacttat
   100801 tttcaagggt atttagatca ttttttggag gaaaattcaa gagatcttta tcaaattcct
   100861 ttttcatcgc ctcagtaatg ttcgggaaaa tcttctcaat aagcatttcc atctggtgac
   100921 gacgtacaga gtccggtgcc tgaagtaatg acagtttctc ggcaacagaa aattcatcag
   100981 taaggccacg aatatcgaaa ctttctggat acgcaattaa agagtggtct tcgtccagat
   101041 ctacccccat ccatttcgca accagtagca tgatttggcg ttcagccctt tccagacgct
   101101 ctgcttttgt gacaagcaag ctatttacac gctggaagtc atacattttt gcggcaccag
   101161 atgaattatc gattccctgt gcgttgtcct gcttcgttcg ctcaccagct acaccaactg
   101221 aatggtagat ttcgttaatc accgtcttaa tcgtagtgat gatcatctga gcttgtttcg
   101281 ggtctggtga cagataaaac ggctggtttc cgccttcaga atcgtaggtg aagactcgct
   101341 ttgtgcccat ttcaagcact ttagtgtggt tttcatcacc aggtaaaagc gactgtaccg
   101401 gtatagccaa ctggctaaat gtctgatcct gaataatggc atcaaggttt gacagatagt
   101461 ttgcaaccgc acgatcaaga taagcgatat catcgatcaa cgatgggctg aaatacggtg
   101521 attcactttc tccaatacaa tcaacaggaa acacaggaac tacgccgaga ttatgctcac
   101581 cgctatcctc taaaataact tttgcctgac ggcgacctgc tccaccagcg cccttcttca
   101641 cttcctcacg gaacagatac cactcgtttt gtgtccacag acgataacgt tggtattcct
   101701 gacctgtaga agtaaaagga tctgcgtcat cacgagcgac ttccacaatt aacgcccaca
   101761 acatattccc gtcgtcgtcc catgccacat ccagcatttg ctgaggtgaa atccagtagg
   101821 cgtaggcgcg agcatctttc tttttctcgt cagctactga ctcaacatca ccactcatcg
   101881 tgctatcgac aacaacccat atgcgaccgt aaatagacga ctgcaaatca atagcggcca
   101941 taaatgagtc aatagaggca ttctggcgag tcgcacgttt ccagaaattg cggatctgct
   102001 ctggtgcctc ttcgatattt ctatgaatgt cttctttaaa gagatatttg ttgatgaggt
   102061 ttaccacctc acgagtgtgg ttgaagcgat aagcacgttc aactcgctcc ttaaactcct
   102121 gatctccctc tttaaagtaa cgaaagatat tgtctgtaaa ccaaccacgc ccgccagcgt
   102181 aagtgctggc gaggaagtcc caatgttctt ttttcttttc gtattccggg tggcgtcgcg
   102241 ccaccagatc cttaatttgt ttgtcgttca attccatttg aataacctta gataattact
   102301 tacctatcga gaaccaccaa gaataacacg gtttttgact ggatacctac gatgtactgg
   102361 atagcccaat gcgtctgcac tatgctcaat gccacctgtc ttatccatat ctcgtgttcc
   102421 aggcttgtag atgacttttt ccagtgaatc aatcaaatgc ttgcacttcg gatcgatata
   102481 cagccggata tctccagatg cggtcatcag cattcggtta acagcattaa cacgatccgc
   102541 aattggcggg tgctttttcg aataatcgac acgtaagaat cccttctctt tgaatatgtc
   102601 gacgtcagac tccccgcgag catgttggcg ataagcacct gccggatctg gaaatatcgt
   102661 tatctgtgat ttccagcgcc agaagcgtct ctcaagctca tcacaaacct ctgccgtgtt
   102721 agaggaaaag agcaccaatt catcaattgc ccatagctca ccatttggct gaggttgcaa
   102781 aattacggaa gacatcgggt caatgttgaa gtcctgccct acccatatag gtaaccgagg
   102841 gttgaactga agcggtttta catgcacgtt acgatcgaac gggtagtaaa cgcgcccgga
   102901 catgttctca aagctggcca ggtactcctg agcgaacgat ttggggtcca tatcgttctt
   102961 agcagcttca atttccgccg taggtacaaa cggagaatcg gcagttacaa actgccagct
   103021 cttccactgt ccctttcttt gcagctctac gttctggcct atagtccaca gtttgtgaaa
   103081 ttccgagaag ccttttggcg taccaatgat cagtgcaccg ccgcgtgtcg atgaaagagt
   103141 aggtcgtaac accttgtacc aagtgtcagc cttcatatcc tgaaattcat caagcactac
   103201 aaaatgcaac gctacgccgc gcagagtgtc aggcttatca gcacctttga gggcgatctc
   103261 cgaaccgttc ttcaacacga ttgtcatcgt ggtgtcgttc tttttcctaa cccacttacg
   103321 aggcagaact tcctgtagat cgtcccacaa aatctggcga gccatctggt aagtaggagc
   103381 gacataccag accctttgct ttttttcctt tgccgccgca cgaataatag tggagatcga
   103441 caaccgggat tttccccagc gtcgaccagc acaaacaact ttaaatcgat gcggagactg
   103501 gaagactttc atctgtccag aatgcagttg cacaagactg agcgaggacg ggattgccat
   103561 tattcgtcct ccccttcact tccatcatct gtcgcatcaa attcgcttag agcttcttct
   103621 tccaacgtct caagcaattc gtcatcgatg atttcaggct cgtcgtcttc ctgacgtaat
   103681 ttcgccacct gggaaggcgt aagctcacca aataccaggt tcggaatttc ttcctcgtca
   103741 ttttccgcat gatccatgcc caatgctttg gacgaaactt caaagcattt tgcaagggta
   103801 ttactggctc tctgtaagct cttgagagaa tcctcaatcg cccctaaagg cttaccctca
   103861 cgtttggccg tagtgacttc gaccatcacc atctgcccca acgcatacgc ccagccgtca
   103921 taacgtgtac gacgttcttc tatcttttcc gcacgggctt tagcgcgaag ctctgcgtca
   103981 gatttaagag actcacgaac catcttccca acagagtccg cgcctttctc taatcctcgc
   104041 tttttgaaat gtctggagag tgtttcacga cgaatgccgt actcttcctc cagctttgag
   104101 agtgtatatt cgcctgacgt ccatttggct tcagcttcgg cccactccgc tggtgtcagg
   104161 cgagttttta cctcgtcttt ttcgaccgtc atagatccct ctaaaacaca cagagcgcgt
   104221 ccatgcgctc taaaacaact tgtttactgc atctgctaac caacttgttt tctggggtgt
   104281 ttaattaggt ctggcatgtc ttacgaagcc tgcttccgta tatatttaat aagttactta
   104341 ttatttatat atacagaagc aggtctttaa ataagctccc agaccgatta catcaccagt
   104401 aacttcgctt tggctcgacc taaagtggtt aacccaagag ttcggcggtg atagcgattg
   104461 tcactgcgtt ggcgcgtatg ccctttctcc accagcccct tttttatcag agcgcgaatt
   104521 gagaactgga tactttgctt agttgtcttg tacggcaaaa cttcaagcaa ttcgtccagg
   104581 tcaagtagat gacctcgctc ataaccgaga ttgagcgttt tgatgatgtc cttttgttta
   104641 tcggttaacg tcatggcaaa tccttatgcc ggtaaagcaa tttctaacgg tttatccaaa
   104701 ggttgtttgt cgaatgccag cagtggcagc gtgtcaggca gtcgacggcc aaagtcaggg
   104761 tttcggtaca caccatacaa cggagacgta aagctcaggt tgtgaatgtc cttgagcagc
   104821 ttcacaatgc tggcctcgtc caccagactg tcggcaatgt cctgaatcgt cgtgccacga
   104881 ttccgcccag cttttgccag ggaactgttc ttgtggtagt ccgccaccag atcacgcagt
   104941 gcacggcgac gacgagactc gctcattgcg aacaactctt tgacgatcgc ctcgttatca
   105001 cccgggtcgg aacggaaatg gcgctggaat acacgcagtg cactttcata gctcttcggt
   105061 cgctcagggc ggatgaactt aaaccctgct ttcatggcga agggattgta tttgctcatc
   105121 gaggactgga tctcaatgat tggccggtca tgcatcctgc taaccaggtt aatcattcga
   105181 taagagacgc ctacgccacg atactgagta tccacaactg agcgactgat caccgcaaag
   105241 ttgttattca cgtaccgacc ccagtactgg ttagccacgg tggtattagt ggttggtttc
   105301 aacttaggaa acatgcggtg gcgaggtgcc agcagtagtt tcgggtaagc cataaccacg
   105361 acgcccacca gacggtcatc aagttcgcag cgatagtaag ttggcgcgaa aggtttgccg
   105421 tctgttttgt agtgaagcga cttaagagcg tgccagtcct ctacagttcc ctttgtgaca
   105481 gtcattcgct ccagaaagtc cagatgacgc ggaaactctt cagggcggta gcgtttaatg
   105541 atgatgtccg tcataaatga acctcatcat gactaccatt gtgatattcc accttcacgc
   105601 gttctttgta gtgcttggtg atctgcatat ccgggcgcag cgcgttcttc aggtcttcgt
   105661 gagtcgtcgc caccattacc gtcgcaccaa cctttcgagc ggcacgctgg aggttagatg
   105721 ccacaacctg agcggttaca cggtctagaa cagcaccgaa ctcgtcagca gcccacactt
   105781 tagcgcctga ctcaatcaat ttggcaatct tgagacgata tttctgaccg tctgacattt
   105841 cagaaggctt gcgaacaaac agatatgcat cgttcagacc agccatagat aacaacccaa
   105901 gcgcatcgct ggtcgttttg cccaactgat cgatgacgtt aacctcatta tcgaaggtaa
   105961 aatcatcgat ggaggctaca gacagccctt catccttcat ctgtcgttgc aactcgcgca
   106021 gcacaacgga tttgccggaa ccggattggc cggtgatgta caccacatcg ccctgcttca
   106081 cttccagctc cagattgtcg taaagcgtcc actctttttc gtccaggcca agcccgaacg
   106141 actcagcgat ttccaacgtg cgcgtggttt tatttacgcg agtctgaaac gatacgttga
   106201 tgatgtattt gctcatgcag ccatctcccc ggaagagatc ttctccgcat atgccacaaa
   106261 tgcgtctacc ccgctttctc ccgtcatttc ttccatgtgg gcaagcaaat caccaacaac
   106321 aatggcagag ccagcaggaa gcgttttaaa gcccaatacg tcgacaacac gtacttcttc
   106381 cgctgcaact tcacgactga tctcggtgtg ttcatccttc tgtcgtttag tttcttcgcc
   106441 aagatcgata actagcgagt cggtgtccat ttcttctgtc atgctgccaa cgagaacatt
   106501 caactcacgc tcttcaaagc cgaaaacctc gatatcgtcc agaacaagcg actcaagctc
   106561 tttctgtagc ttaattgcat cgtaatcaat gctggcaagt cggttatctt caaggcgctt
   106621 cgcacgaacc tcgtcatcac tgagatcatc gcgaacaata accggtacgc gctcaagtcc
   106681 agcaaaaatt gcagcctcac ggcggccgtg gccagtaata attacgtcgt tcttatcgac
   106741 cgtaattggc tggtcaaatc cgcgcttttt aatggctgcg gccagatctc ggatctgctg
   106801 ttcatcatgc tttttggcat tcatctcata gggaataaga tctgccgggt cgcgatatac
   106861 gatttcaaac tttttggtca ttacatacgc tccttgtagt agtcgaccag ccacaccaga
   106921 gcctcaccag cgttctccat ttcattaccg gtgttaatag cctgctcttt gatgatgttt
   106981 tttatggttt ctgcaacacg atctgacgca tcgaaagtta ctttgaagcg catggtctga
   107041 tgttccgcac ccacacgttc ggttttctct cgtttgtcgg tatcgacagg ctcatcacta
   107101 ccacgagaca acgcctccag tgcttcaagg tcgattgccg cctcttttgc taaaaccatc
   107161 gagatttcgt cgtcatacgg ggcgatttca gacagttgat agtcaagttc tgactgaatt
   107221 tcttcaatga agcgttgcaa tgcgatttgg tcgtcttcac cgtatcgctc gttgtccacc
   107281 agtgacatct gtttagctac gacatcgcta attttgccca cagaaagcac cggaaccgtt
   107341 gaaattcctt gctcaatagc agcacgccag cgatgttcgc cgccgaggat ttcaaaaaat
   107401 ccatcttcaa gttcacgagc caaaattggc ttaaaaaagc ccaatttttc gatagaacct
   107461 ttcagttttt caaaattctg cgcaccaacc gaattggtgt tccaggtatt cgggcgaagg
   107521 ttggcaacat caacctgcaa aatcgtgatt tttacatcca ttttattgct acaatccact
   107581 aagtaatcac ttacttatta taatagccaa ataacataca aaaggcacta aggaaagagg
   107641 tttatgactg ttcggattgt atctaacgca gtaaatgcgc ttatttctgg cgcagatgac
   107701 aaggtaaagc aactggtgca gcaaatgttg agctacgaag tcgagactgg cgactggaag
   107761 ggcacaagca cgatgttcaa ctggagtaaa aactcgttcc ctgctggctt tgccaagcct
   107821 gtagcggcga acttgaacaa ggcgggcatc aaatgtgttc atatccgcaa agacaaagcc
   107881 ccggcgcttg gtaaaccaaa tccggcggtt aacccattcc catacaatcc tgattatgcg
   107941 tatcaggatc agactgtgga aacactggtt cgagagggaa tgatgattgc gcagatcgct
   108001 actggtggcg ggaaatctaa tgttgcctgc aaagcagctg cacgtatcgg tcgaatgaca
   108061 ttatttttaa caacccgctc tgttctgatg tttcaaatgg ccgaaaactt ccagagatcc
   108121 atcgactacc gcgccaaaaa tggcgaaccg tggttaaaag accaaaaggt tggagtcatt
   108181 ggctcgggtg agttccaggt atcacgacat atcaacgtcg ctacagttca aactcttgca
   108241 agtttcctcg aagaaccacc acgcgatgca acaccagata agaaaagcta ccacctcaaa
   108301 cgtcgggagt tggtgaaacg cttcctttca agtgtctctc ttcttattct ggaagaggcg
   108361 catgagtctt caggctcaaa tttctatgac atcgccagat tatgtgtgaa cgcagactat
   108421 cgtctggcgc ttacagccac gccgttcatg aaggattcga cggaagccaa catgcgcctg
   108481 atggcggtgg ccgggcgaat tgaaattaaa gtcacagaaa agtacctgat tgatcgaggc
   108541 attctggcaa agccgtactt cctttatcat aaagttgcct acaagccaga cgaggccaga
   108601 atcaaggccg aacttgccaa caaacatctc aattttagag ttggtatgag caccgcctac
   108661 caaaaggctt atcagttggg gatcgtgtat aatttgggac gtaacgaggc catagtgcgc
   108721 gaggcattgc tttataagca acattcactc aactgcatga ctctggttcg tcttaaacgc
   108781 cacgggcaaa tcctgatgga gatgatgaag gagtcaggtc ttagagttga tttcatctat
   108841 ggggaatcta accagacgac aaggcaagca aagctgaaca gtttagcgtc tggtgaaata
   108901 gatgttttaa taggctcgac tattctggat gttggtgttg atgtgccaag cgttggtgcg
   108961 gtcattcttg ctggtggtgg gaaagcagaa gttgaaatga gacaacgtgt cggtcgtggc
   109021 ttacgagcca aaaaaaatca ggcaaacgtg tgttttatca ctgatttcat tgacattagc
   109081 aacaaatacc tgttgtctca ctcttatgag cgaaaacaca tcatcgacac cacacctggc
   109141 tttgcagaag gtgtattgcc tattgatgga gcattcgatt ttggagttct gaaacgagat
   109201 tagttatgag tgaaaagaaa acaacttact gtcaggtagc attgtctgat aaggccaatg
   109261 acaaacttgg aaagtttcaa gtgaaactaa aagaaaaaaa tatcaaaatg tctaaggctg
   109321 aagtcattaa taccattctg gaacaattga caatggccga ctttgacaag gttatatctt
   109381 ctgtcggggc ttccgctaag actcgtgaga aaatcatgcg tatctatgag aactctaata
   109441 tgacaaagga agatctcgaa acgctattaa gcagattaaa ataatcacgc tatcaattag
   109501 ggagacaaag atgaagtctc ccatactgtt cactttctgt tctaaaacta acatgaaata
   109561 ctcgcttctc cctctactaa cgttaccagt gttgatgctt accgcgtgcc aatcacgtcc
   109621 aatatcaatc catgacgcca aaccagcacc gcaggccaaa gtgttcaagt atcaaagcgc
   109681 agcgccagct acgttggtgg taatgagaga cacaggtttt gtaggagctg gatgtgatgc
   109741 ttccattttc atcaatggcg aaactgtcgc aaaactggct acaggcgaaa aagcgacttt
   109801 ccatttaaat gccggagaat taattgtggg tgcatctctt gaaggtgcag gtttatgtgc
   109861 tctaaacccc gctcgtcaag agcgagaaac gactctgaag aatggagaca caaaagcgtt
   109921 cagggtgttc accagcaatt ctggtaacat cgacattctg ccaacaactc tgtgatgaca
   109981 tgacaaccaa agatattacc tacggtatcc aagctgaact cttgccgcga gattacacta
   110041 acgttgaaaa actactgatg ttctggcgca gagaacaaat tcctgtaagg gtcactctcg
   110101 aagatggtca ggcgttttgc atgtacgttt atggcctcat gccatctcgc aacaaagttg
   110161 acctttgccc agcccctttt gacaaagaaa atcgtataag gctcccactt gaacgcatta
   110221 gtacaattga atcaggtgtg gttgacagta tcgctcacga tttcaaaggt cgattaacag
   110281 ttcaccctga ttatgtcgat aatcggccat cacgccgcga tttttttgca atttgcaacc
   110341 aagcctacaa agcaaacaaa tctataaggg tgtacatggc ggatggccgt gaaattgagg
   110401 gggtgtcagc aggcgcagat gcttgtcagg ttacactacg tgtcgagaac ggtagaaaaa
   110461 tagttgtttt gttcgattgg gttgaacgaa ttttgccttt ttgagttatg aaatcgatat
   110521 tgttaccacc attattttta ctttgctcag ttgcggcaaa tgcaatggac tataagcctg
   110581 tcattcagtc gctgatgaat gacgtgtgct caacgtctca gaatgtatca gtttgcatgt
   110641 atcaattttc ggcagccgta aaagcaggaa aagcgatagg tgagaatgtg gaactgtgta
   110701 agaaagtggc aaatgaagaa cgggcaatgt tggattgcga atctagcgag tcatcggcac
   110761 agttcgttga tgcgctattt gacaccaatc gtaaggctgt agagtctgtt caataaatct
   110821 ataaggttaa taaccggcta agtccggtta ttatttttca catcacttct ttatatctac
   110881 aaatcaaata acctaaatat atataaggtt aaaagcccga attgattatt tatttaggga
   110941 acaccttcga cgatctcgct tttatttcta ggactttcat ccctgcaaaa aattt
//
LOCUS       DAOFMX010000003        95532 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.003, whole genome shotgun sequence.
ACCESSION   DAOFMX010000003 DAOFMX010000000
VERSION     DAOFMX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.003"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(87..1337)
                     /gene="icd"
                     /locus_tag="QBA20_000262"
     CDS             complement(87..1337)
                     /gene="icd"
                     /locus_tag="QBA20_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDQ6290944.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1509..2162
                     /gene="rluE"
                     /locus_tag="QBA20_000263"
     CDS             1509..2162
                     /gene="rluE"
                     /locus_tag="QBA20_000263"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDQ6290945.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGTELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2172..2633
                     /gene="nudJ"
                     /locus_tag="QBA20_000264"
     CDS             2172..2633
                     /gene="nudJ"
                     /locus_tag="QBA20_000264"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDQ6290946.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2687..3793
                     /gene="mnmA"
                     /locus_tag="QBA20_000265"
     CDS             2687..3793
                     /gene="mnmA"
                     /locus_tag="QBA20_000265"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDQ6290947.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3829..4470
                     /gene="hflD"
                     /locus_tag="QBA20_000266"
     CDS             3829..4470
                     /gene="hflD"
                     /locus_tag="QBA20_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDQ6290948.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4474..5844
                     /gene="purB"
                     /locus_tag="QBA20_000267"
     CDS             4474..5844
                     /gene="purB"
                     /locus_tag="QBA20_000267"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDQ6290949.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6013..6684
                     /gene="phoP"
                     /locus_tag="QBA20_000268"
     CDS             6013..6684
                     /gene="phoP"
                     /locus_tag="QBA20_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDQ6290950.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6684..8144
                     /gene="phoQ"
                     /locus_tag="QBA20_000269"
     CDS             6684..8144
                     /gene="phoQ"
                     /locus_tag="QBA20_000269"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDQ6290951.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8941..9222)
                     /locus_tag="QBA20_000270"
     CDS             complement(8941..9222)
                     /locus_tag="QBA20_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290952.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9236..10897)
                     /locus_tag="QBA20_000271"
     CDS             complement(9236..10897)
                     /locus_tag="QBA20_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDQ6290953.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10881..11207)
                     /locus_tag="QBA20_000272"
     CDS             complement(10881..11207)
                     /locus_tag="QBA20_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDQ6290954.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11263..11445)
                     /locus_tag="QBA20_000273"
     CDS             complement(11263..11445)
                     /locus_tag="QBA20_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290955.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11429..11869)
                     /locus_tag="QBA20_000274"
     CDS             complement(11429..11869)
                     /locus_tag="QBA20_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDQ6290956.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11869..12165)
                     /locus_tag="QBA20_000275"
     CDS             complement(11869..12165)
                     /locus_tag="QBA20_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDQ6290957.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12162..12500)
                     /locus_tag="QBA20_000276"
     CDS             complement(12162..12500)
                     /locus_tag="QBA20_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDQ6290958.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12497..13708)
                     /locus_tag="QBA20_000277"
     CDS             complement(12497..13708)
                     /locus_tag="QBA20_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDQ6290959.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVDQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13710..14282)
                     /locus_tag="QBA20_000278"
     CDS             complement(13710..14282)
                     /locus_tag="QBA20_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDQ6290960.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDPSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14322..15479)
                     /locus_tag="QBA20_000279"
                     /pseudo
     CDS             complement(14322..15479)
                     /locus_tag="QBA20_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15780..16004)
                     /locus_tag="QBA20_000280"
     CDS             complement(15780..16004)
                     /locus_tag="QBA20_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290961.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16146..16307)
                     /locus_tag="QBA20_000281"
     CDS             complement(16146..16307)
                     /locus_tag="QBA20_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDQ6290962.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16317..16622)
                     /locus_tag="QBA20_000282"
     CDS             complement(16317..16622)
                     /locus_tag="QBA20_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290963.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16619..16897)
                     /locus_tag="QBA20_000283"
     CDS             complement(16619..16897)
                     /locus_tag="QBA20_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290964.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17186..18934)
                     /locus_tag="QBA20_000284"
     CDS             complement(17186..18934)
                     /locus_tag="QBA20_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDQ6290965.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIAILLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18931..19230)
                     /locus_tag="QBA20_000285"
     CDS             complement(18931..19230)
                     /locus_tag="QBA20_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDQ6290966.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19236..19469)
                     /locus_tag="QBA20_000286"
     CDS             complement(19236..19469)
                     /locus_tag="QBA20_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290967.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19471..19691)
                     /locus_tag="QBA20_000287"
                     /pseudo
     CDS             complement(19471..19691)
                     /locus_tag="QBA20_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19684..19905)
                     /locus_tag="QBA20_000288"
     CDS             complement(19684..19905)
                     /locus_tag="QBA20_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290968.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19895..20095)
                     /locus_tag="QBA20_000289"
     CDS             complement(19895..20095)
                     /locus_tag="QBA20_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDQ6290969.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20225..20404
                     /locus_tag="QBA20_000290"
     CDS             20225..20404
                     /locus_tag="QBA20_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290970.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20529..20858
                     /locus_tag="QBA20_000291"
     CDS             20529..20858
                     /locus_tag="QBA20_000291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6290971.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20908..21096)
                     /locus_tag="QBA20_000292"
     CDS             complement(20908..21096)
                     /locus_tag="QBA20_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDQ6290972.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21471..22700)
                     /locus_tag="QBA20_000293"
     CDS             complement(21471..22700)
                     /locus_tag="QBA20_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6290973.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLNLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22949..24070
                     /gene="roxA"
                     /locus_tag="QBA20_000294"
                     /gene_synonym="ycfD"
     CDS             22949..24070
                     /gene="roxA"
                     /locus_tag="QBA20_000294"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDQ6290974.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24119..25345)
                     /gene="pepT"
                     /locus_tag="QBA20_000295"
     CDS             complement(24119..25345)
                     /gene="pepT"
                     /locus_tag="QBA20_000295"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDQ6290975.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25595..26731
                     /gene="potA"
                     /locus_tag="QBA20_000296"
     CDS             25595..26731
                     /gene="potA"
                     /locus_tag="QBA20_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDQ6290976.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26715..27572
                     /gene="potB"
                     /locus_tag="QBA20_000297"
     CDS             26715..27572
                     /gene="potB"
                     /locus_tag="QBA20_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDQ6290977.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27569..28363
                     /gene="potC"
                     /locus_tag="QBA20_000298"
     CDS             27569..28363
                     /gene="potC"
                     /locus_tag="QBA20_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDQ6290978.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28360..29406
                     /gene="potD"
                     /locus_tag="QBA20_000299"
     CDS             28360..29406
                     /gene="potD"
                     /locus_tag="QBA20_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDQ6290979.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29562..30383)
                     /gene="cobB"
                     /locus_tag="QBA20_000300"
     CDS             complement(29562..30383)
                     /gene="cobB"
                     /locus_tag="QBA20_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDQ6290980.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30399..31310)
                     /gene="nagK"
                     /locus_tag="QBA20_000301"
     CDS             complement(30399..31310)
                     /gene="nagK"
                     /locus_tag="QBA20_000301"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDQ6290981.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31339..32583)
                     /gene="lolE"
                     /locus_tag="QBA20_000302"
     CDS             complement(31339..32583)
                     /gene="lolE"
                     /locus_tag="QBA20_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDQ6290982.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32583..33284)
                     /gene="lolD"
                     /locus_tag="QBA20_000303"
     CDS             complement(32583..33284)
                     /gene="lolD"
                     /locus_tag="QBA20_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDQ6290983.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33277..34476)
                     /gene="lolC"
                     /locus_tag="QBA20_000304"
     CDS             complement(33277..34476)
                     /gene="lolC"
                     /locus_tag="QBA20_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDQ6290984.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34738..35811
                     /locus_tag="QBA20_000305"
     CDS             34738..35811
                     /locus_tag="QBA20_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDQ6290985.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFIYTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35939..39385
                     /gene="mfd"
                     /locus_tag="QBA20_000306"
     CDS             35939..39385
                     /gene="mfd"
                     /locus_tag="QBA20_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDQ6290986.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39528..40490
                     /gene="ldtC"
                     /locus_tag="QBA20_000307"
     CDS             39528..40490
                     /gene="ldtC"
                     /locus_tag="QBA20_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDQ6290987.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40573..40785)
                     /locus_tag="QBA20_000308"
     CDS             complement(40573..40785)
                     /locus_tag="QBA20_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ6290988.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41070..41702
                     /gene="comR"
                     /locus_tag="QBA20_000309"
     CDS             41070..41702
                     /gene="comR"
                     /locus_tag="QBA20_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDQ6290989.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41764..42302)
                     /locus_tag="QBA20_000310"
                     /pseudo
     CDS             complement(41764..42302)
                     /locus_tag="QBA20_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
     gene            complement(42529..43833)
                     /gene="ndh"
                     /locus_tag="QBA20_000311"
     CDS             complement(42529..43833)
                     /gene="ndh"
                     /locus_tag="QBA20_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDQ6290990.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44241..44783)
                     /locus_tag="QBA20_000312"
     CDS             complement(44241..44783)
                     /locus_tag="QBA20_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ6290991.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44806..45831)
                     /gene="nagZ"
                     /locus_tag="QBA20_000313"
     CDS             complement(44806..45831)
                     /gene="nagZ"
                     /locus_tag="QBA20_000313"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDQ6290992.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(45842..46666)
                     /gene="thiK"
                     /locus_tag="QBA20_000314"
     CDS             complement(45842..46666)
                     /gene="thiK"
                     /locus_tag="QBA20_000314"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDQ6290993.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46647..47288)
                     /gene="lpoB"
                     /locus_tag="QBA20_000315"
     CDS             complement(46647..47288)
                     /gene="lpoB"
                     /locus_tag="QBA20_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDQ6290994.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47303..47680)
                     /locus_tag="QBA20_000316"
     CDS             complement(47303..47680)
                     /locus_tag="QBA20_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDQ6290995.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47683..48042)
                     /gene="hinT"
                     /locus_tag="QBA20_000317"
     CDS             complement(47683..48042)
                     /gene="hinT"
                     /locus_tag="QBA20_000317"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDQ6290996.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48381..50566
                     /gene="fhuE"
                     /locus_tag="QBA20_000318"
                     /pseudo
     CDS             48381..50566
                     /gene="fhuE"
                     /locus_tag="QBA20_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50626..52059)
                     /gene="ptsG"
                     /locus_tag="QBA20_000319"
     CDS             complement(50626..52059)
                     /gene="ptsG"
                     /locus_tag="QBA20_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDQ6290997.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52354..53151)
                     /locus_tag="QBA20_000320"
     CDS             complement(52354..53151)
                     /locus_tag="QBA20_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDQ6290998.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53162..54166)
                     /gene="holB"
                     /locus_tag="QBA20_000321"
     CDS             complement(53162..54166)
                     /gene="holB"
                     /locus_tag="QBA20_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDQ6290999.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54163..54804)
                     /locus_tag="QBA20_000322"
     CDS             complement(54163..54804)
                     /locus_tag="QBA20_000322"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDQ6291000.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54794..55816)
                     /gene="yceG"
                     /locus_tag="QBA20_000323"
     CDS             complement(54794..55816)
                     /gene="yceG"
                     /locus_tag="QBA20_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDQ6291001.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55819..56628)
                     /gene="pabC"
                     /locus_tag="QBA20_000324"
     CDS             complement(55819..56628)
                     /gene="pabC"
                     /locus_tag="QBA20_000324"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDQ6291002.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56748..57989)
                     /gene="fabF"
                     /locus_tag="QBA20_000325"
     CDS             complement(56748..57989)
                     /gene="fabF"
                     /locus_tag="QBA20_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDQ6291003.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58076..58312)
                     /gene="acpP"
                     /locus_tag="QBA20_000326"
     CDS             complement(58076..58312)
                     /gene="acpP"
                     /locus_tag="QBA20_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDQ6291004.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58523..59257)
                     /gene="fabG"
                     /locus_tag="QBA20_000327"
     CDS             complement(58523..59257)
                     /gene="fabG"
                     /locus_tag="QBA20_000327"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDQ6291005.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59270..60199)
                     /gene="fabD"
                     /locus_tag="QBA20_000328"
     CDS             complement(59270..60199)
                     /gene="fabD"
                     /locus_tag="QBA20_000328"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDQ6291006.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60215..61168)
                     /gene="fabH"
                     /locus_tag="QBA20_000329"
     CDS             complement(60215..61168)
                     /gene="fabH"
                     /locus_tag="QBA20_000329"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDQ6291007.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61236..62306)
                     /gene="plsX"
                     /locus_tag="QBA20_000330"
     CDS             complement(61236..62306)
                     /gene="plsX"
                     /locus_tag="QBA20_000330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDQ6291008.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62387..62560)
                     /gene="rpmF"
                     /locus_tag="QBA20_000331"
     CDS             complement(62387..62560)
                     /gene="rpmF"
                     /locus_tag="QBA20_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDQ6291009.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62612..63133)
                     /gene="yceD"
                     /locus_tag="QBA20_000332"
     CDS             complement(62612..63133)
                     /gene="yceD"
                     /locus_tag="QBA20_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDQ6291010.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63332..63916
                     /gene="yceF"
                     /locus_tag="QBA20_000333"
     CDS             63332..63916
                     /gene="yceF"
                     /locus_tag="QBA20_000333"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDQ6291011.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64028..64987)
                     /gene="rluC"
                     /locus_tag="QBA20_000334"
     CDS             complement(64028..64987)
                     /gene="rluC"
                     /locus_tag="QBA20_000334"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDQ6291012.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65560..68745
                     /gene="rne"
                     /locus_tag="QBA20_000335"
     CDS             65560..68745
                     /gene="rne"
                     /locus_tag="QBA20_000335"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDQ6291013.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQSTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68942..69895)
                     /gene="flgL"
                     /locus_tag="QBA20_000336"
     CDS             complement(68942..69895)
                     /gene="flgL"
                     /locus_tag="QBA20_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDQ6291014.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(69907..71549)
                     /gene="flgK"
                     /locus_tag="QBA20_000337"
                     /pseudo
     CDS             complement(69907..71549)
                     /gene="flgK"
                     /locus_tag="QBA20_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71615..72556)
                     /gene="flgJ"
                     /locus_tag="QBA20_000338"
     CDS             complement(71615..72556)
                     /gene="flgJ"
                     /locus_tag="QBA20_000338"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDQ6291015.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72556..73653)
                     /gene="flgI"
                     /locus_tag="QBA20_000339"
     CDS             complement(72556..73653)
                     /gene="flgI"
                     /locus_tag="QBA20_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDQ6291016.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73665..74363)
                     /gene="flgH"
                     /locus_tag="QBA20_000340"
     CDS             complement(73665..74363)
                     /gene="flgH"
                     /locus_tag="QBA20_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDQ6291017.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74416..75198)
                     /gene="flgG"
                     /locus_tag="QBA20_000341"
     CDS             complement(74416..75198)
                     /gene="flgG"
                     /locus_tag="QBA20_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDQ6291018.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75336..76087)
                     /gene="flgF"
                     /locus_tag="QBA20_000342"
                     /pseudo
     CDS             complement(75336..76087)
                     /gene="flgF"
                     /locus_tag="QBA20_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76107..77315)
                     /gene="flgE"
                     /locus_tag="QBA20_000343"
     CDS             complement(76107..77315)
                     /gene="flgE"
                     /locus_tag="QBA20_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDQ6291019.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(77340..78035)
                     /gene="flgD"
                     /locus_tag="QBA20_000344"
     CDS             complement(77340..78035)
                     /gene="flgD"
                     /locus_tag="QBA20_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDQ6291020.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(78047..78450)
                     /gene="flgC"
                     /locus_tag="QBA20_000345"
                     /pseudo
     CDS             complement(78047..78450)
                     /gene="flgC"
                     /locus_tag="QBA20_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78454..78870)
                     /gene="flgB"
                     /locus_tag="QBA20_000346"
     CDS             complement(78454..78870)
                     /gene="flgB"
                     /locus_tag="QBA20_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDQ6291021.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            79025..79684
                     /gene="flgA"
                     /locus_tag="QBA20_000347"
     CDS             79025..79684
                     /gene="flgA"
                     /locus_tag="QBA20_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDQ6291022.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            79760..80053
                     /gene="flgM"
                     /locus_tag="QBA20_000348"
     CDS             79760..80053
                     /gene="flgM"
                     /locus_tag="QBA20_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDQ6291023.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            80058..80474
                     /gene="flgN"
                     /locus_tag="QBA20_000349"
     CDS             80058..80474
                     /gene="flgN"
                     /locus_tag="QBA20_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDQ6291024.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80514..82049)
                     /gene="murJ"
                     /locus_tag="QBA20_000350"
     CDS             complement(80514..82049)
                     /gene="murJ"
                     /locus_tag="QBA20_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDQ6291025.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82216..83082)
                     /locus_tag="QBA20_000351"
     CDS             complement(82216..83082)
                     /locus_tag="QBA20_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDQ6291026.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(83084..83731)
                     /locus_tag="QBA20_000352"
     CDS             complement(83084..83731)
                     /locus_tag="QBA20_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDQ6291027.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLYSLLAHLGD"
     gene            complement(83742..84326)
                     /gene="rimJ"
                     /locus_tag="QBA20_000353"
     CDS             complement(83742..84326)
                     /gene="rimJ"
                     /locus_tag="QBA20_000353"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDQ6291028.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84562..85770
                     /gene="mdtH"
                     /locus_tag="QBA20_000354"
     CDS             84562..85770
                     /gene="mdtH"
                     /locus_tag="QBA20_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDQ6291029.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85834..86481
                     /gene="grxB"
                     /locus_tag="QBA20_000355"
     CDS             85834..86481
                     /gene="grxB"
                     /locus_tag="QBA20_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDQ6291030.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86615..87175
                     /locus_tag="QBA20_000356"
     CDS             86615..87175
                     /locus_tag="QBA20_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ6291031.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87281..88327
                     /gene="pyrC"
                     /locus_tag="QBA20_000357"
     CDS             87281..88327
                     /gene="pyrC"
                     /locus_tag="QBA20_000357"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDQ6291032.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88401..88646
                     /gene="dinI"
                     /locus_tag="QBA20_000358"
     CDS             88401..88646
                     /gene="dinI"
                     /locus_tag="QBA20_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDQ6291033.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88936..89190
                     /gene="bssS"
                     /locus_tag="QBA20_000359"
     CDS             88936..89190
                     /gene="bssS"
                     /locus_tag="QBA20_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDQ6291034.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89305..90423
                     /gene="solA"
                     /locus_tag="QBA20_000360"
     CDS             89305..90423
                     /gene="solA"
                     /locus_tag="QBA20_000360"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDQ6291035.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90471..90584
                     /locus_tag="QBA20_000361"
     CDS             90471..90584
                     /locus_tag="QBA20_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDQ6291036.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90845..91411
                     /locus_tag="QBA20_000362"
     CDS             90845..91411
                     /locus_tag="QBA20_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDQ6291037.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91415..91990
                     /locus_tag="QBA20_000363"
     CDS             91415..91990
                     /locus_tag="QBA20_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDQ6291038.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(92032..93084)
                     /locus_tag="QBA20_000364"
     CDS             complement(92032..93084)
                     /locus_tag="QBA20_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDQ6291039.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93309..94229
                     /gene="lpxL"
                     /locus_tag="QBA20_000365"
     CDS             93309..94229
                     /gene="lpxL"
                     /locus_tag="QBA20_000365"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDQ6291040.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94401..94598
                     /locus_tag="QBA20_000366"
     CDS             94401..94598
                     /locus_tag="QBA20_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDQ6291041.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94622..>95532
                     /locus_tag="QBA20_000367"
     CDS             94622..>95532
                     /locus_tag="QBA20_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDQ6291042.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT        22978 a        25309 c        23584 g        23661 t
ORIGIN      
        1 atgctcgata tgctattaat aatatccaca taaataaaac aacgggcgtg ttatacgccc
       61 gtttaaatat ttaacacatg tagtgattac atattcttga tgatcgcatc accaaactct
      121 gaacatttca gcagtttagc gccttccatc agacgctcga agtcataggt tacggtcttg
      181 gcgttgatcg cgccttccat acctttaaca atcaggtctg cggcttcagt ccagcccata
      241 tggcgtaaca tcatctcagc ggagagaata atagagccag ggtttacttt gtcctgaccg
      301 gcgtatttcg gcgcagtacc gtgggtggct tcaaacaggg cgcattcgtc accgatgttt
      361 gctccagggg cgataccgat accgccaacc tgcgctgcca gagcgtcaga aatgtagtca
      421 ccgttcaggt tcatacaggc gataacatca tattcagccg gacgcagcag gatctgttgc
      481 aggaatgcat cagcaatcac gtctttaatg acgatctctt tgccggtatt cgggttttta
      541 actttcagcc acgggccgcc gtcgatcagt tcaccgccaa attcttcacg cgccagctgg
      601 tagccccagt ctttaaacgc gccttcggtg aacttcatga tgttgccttt gtgcaccagg
      661 gtcacagagt cacgatcgtt agcaattgcg tattcgatcg ctgcacgaac cagacgtttg
      721 gtgccttctt cagaacacgg cttaataccg ataccgcaat gttccgggaa gcgaattttc
      781 ttcacgccca tctcttcacg caggaattta atcactttct cggcgtcggc agagtcagct
      841 ttccattcga tacccgcata aatgtcttcc gagttttcac ggaagataac catatcggtc
      901 agttcagggt gtttaaccgg gcttggagtg ccctgatagt aacgtaccgg acgcaggcag
      961 atgtagagat ccagttcctg acgcagggca acgttgagag aacgaatacc gccaccaacc
     1021 ggagtggtca gcgggccttt aatggcaacg cgatattcac gaatcagatc aagggtttca
     1081 gcaggcagcc agacatcctg accataaacc tgtgtggatt tttcaccggt gtaaatttcc
     1141 atccaggaga ttttacgctc gcctttatag gctttctcga ctgcagcgtc gaccactttc
     1201 agcatggctg gggttacatc tacaccgatt ccatcacctt caatgtaagg gataatcgga
     1261 ttttcaggaa cgttgagttt gccgttttgc agggtgatct tcttgccttg tgccggaaca
     1321 actactttac tttccattca cctctccttc gagcgctact ggtttgctcg tctgccacca
     1381 cgttgcatat gcgtttgcgt cctgcgatac ggatgcttta gagcaatttt ttgttaatga
     1441 tttgtaattg acttgtcaat actaccctat tgtttgccgt catgaaagat gcgcgttatt
     1501 aggctataat gcggcaattc ataatctctg aaaataccat gcaaaaaact tcttttagaa
     1561 atcaccaggt taagcgattc agctcgcaac gttctaccag gcgtaaacat gaaaaccagc
     1621 ccacgcgtgt gatcctcttc aataaaccct acgatgttct tccgcagttc accgatgaaa
     1681 ccggacgcaa aacattaaaa gaattcatcc cggttcaggg cgtttatgca gcaggtcgcc
     1741 ttgaccgcga tagcgaaggg ttgctggtgc tgaccaataa cggcgcgttg caggcgcgtt
     1801 taacccagcc gggtaaacgc accggaaaaa tctattatgt gcaggtggaa ggtattccca
     1861 cacaagacgc acttgaagcc ttgcgcaatg gcgtaacctt aaatgatggc cctaccctgc
     1921 ccgcaggcac ggagctggtt gacgaacccg cgtggttatg gccgcggaat ccaccaattc
     1981 gtgaacgcaa aagtattccc accagctggc tgaagatcac cttatatgaa ggacgtaatc
     2041 gccaggtgcg ccgcatgacc gcccatgttg gtttccccac gctgcgactg attcgctatg
     2101 cgatgggaga ttactctttg gataatcttg ccaatggcga atggcgagaa gtgacagatt
     2161 aaggatgcaa aatgtttaaa ccgcacgtta ccgttgcttg cgtggtgcac gcagaaggca
     2221 aatttttagt cgttgaagag acgattaatg gtaaagcgtt atggaaccaa cctgccgggc
     2281 atctggaagc cgatgaaacc ttagtggaag ccgccgcccg tgaactgtgg gaagaaaccg
     2341 gcatcagcgc gcagccgcaa cactttattc gtatgcatca gtggattgcg ccagataaaa
     2401 cgccgttttt gcgtttcctc tttgccattg agcttgagca aatatgcccg acccagcctc
     2461 atgacagcga tatcgactgc tgccgttggg tcagcgccga agaaatttta caggcgtcaa
     2521 atcttcgttc gccgctggtg gcggaaagta ttcgttgtta tcaaagcggg caacgttatc
     2581 cgctggagat gattggcgat tttaactggc cttttacaaa gggtgtcatc taaaaggctg
     2641 cgtgatagaa tacgccgcct tgaagttcaa tgtcgtgagt gatccaatgt ctgaaaccgc
     2701 aaaaaaagta atcgtcggca tgtccggcgg tgtcgattcc tccgtttctg cctggctgtt
     2761 gcaacaacag ggatatcagg tcgaaggcct gtttatgaag aactgggaag aagacgacgg
     2821 tgaggaatat tgcacagcgg cagcggatct ggctgatgcc caggcggtct gcgacaagct
     2881 cggcattgaa ctgcacaccg ttaactttgc tgccgagtac tgggacaacg tcttcgaact
     2941 gttccttgcc gaatataaag ccggtcgcac gccgaatccg gatattctgt gcaacaagga
     3001 gatcaaattt aaagccttcc tcgaatttgc cgccgaagat ttaggtgccg attatatcgc
     3061 taccggtcat tacgtacgtc gggccgatgt cgatggcaag agccgcctgc tgcgtggtct
     3121 ggacagcaat aaagaccaga gctacttcct ttatacgctc agccatgagc agattgcgca
     3181 aagcctgttc ccggtaggcg aactggaaaa accgcaggtg cgtaagattg ctgaagatct
     3241 tggtctggtc accgcgaaga aaaaagactc taccggcatt tgcttcattg gcgaacgtaa
     3301 attccgcgag ttcctgggcc gttatctccc ggcgcaaccg ggcaaaatca ttaccgtcga
     3361 tggcgatgaa attggtgagc accaggggct gatgtatcac actctcggtc agcgtaaagg
     3421 tctgggtatc ggtggcacca aagaaggcac cgaagaaccg tggtatgtgg tggacaaaga
     3481 cgtcgaaaac aacattctgg ttgtcgctca gggccatgaa cacccgcggc tgatgtctgt
     3541 cgggttgatt gcccagcagc tgcactgggt cgatcgcgaa ccattcaccg gcactatgcg
     3601 ttgcacggta aaaacccgct atcgccagac cgacattccg tgcaccgtca aggcgctgga
     3661 cgacgatcgc attaaagtga ttttcgatga gccggttgcc gccgtgacgc cgggccagtc
     3721 tgccgtcttc tataacggtg aagtgtgcct cggtggcggt attattgagc agcgtctgcc
     3781 gctgccggtc tgattattat ctttacttaa aacaaggaag cagtgaacgt ggcaaagaat
     3841 tactatgaca tcaccctcgc cctggccggt atttgtcagt cggcacgcct ggtgcaacaa
     3901 ctcgctcacc aggggcattg tgatgccgat gcgctacacg tctcactcaa cagtattatt
     3961 gatatgaacc ccagctcgac gctggcggtt tttggcggta gcgaagccaa cctgcgcgtc
     4021 gggctggaaa ccctgctcgg cgtgctcaat gccagcagtt gccagggctt aaacgccgaa
     4081 ttaacccgct acacactcag cttgatggtg cttgagcgca aactctcctc agcgaaaggc
     4141 gcgctcgaca ctctgggcaa ccggatcaac ggcctgcaac gccagctcga acacttcgat
     4201 ttacagtccg aaacgctgat gagcgcgatg gctgctatct atgttgatgt gatcagcccg
     4261 cttggcccgc gcattcaggt caccggttcc cctgctgtac tgcaaagccc acaagtgcag
     4321 gcgaaagttc gcgcaaccct gctggcaggc attcgcgccg ccgtgctctg gcaccaggtc
     4381 ggcggcggac gtctacaact gatgttttct cgtaatcgcc tgaccactca ggcaaaacaa
     4441 attcttgctc atttaacccc ggagttgtga tctatggaat tatcctcact gaccgccgtt
     4501 tcccctgtcg atggacgcta cggcgataaa gtcagcgcgc tgcgcgggat tttcagcgaa
     4561 tatggtttgc tgaaattccg tgtacaagtt gaagtacgtt ggctgcaaaa actggccgcg
     4621 cacgcagcga tcaaggaagt tcctgctttt gctgccgacg caatcggtta ccttgatgca
     4681 attgtcgcca atttcagcga agaagatgcc gcacgcatca aaaccatcga gcgtaccact
     4741 aaccacgacg ttaaagcggt tgagtatttc ctgaaagaaa aagtggcgga gatcccggaa
     4801 ctgcacgcgg tttctgaatt catccacttt gcctgtactt cggaagatat caataacctc
     4861 tcccacgcat taatgctgaa aaccgcgcgt gatgaagtga tcctgccgta ctggcgtcaa
     4921 ctgattgatg gcattaaaga tctcgccgtt cagtaccgcg atatcccgct gctgtctcgt
     4981 acccacggtc agccagccac gccgtcaacc atcggtaaag agatggcaaa cgtcgcctac
     5041 cgtatggagc gccagcaccg ccagcttaac caggtggaga tcctcggcaa aatcaacggc
     5101 gcggtcggta actataacgc ccacatcgcc gcttacccgg aagttgactg gcatcagttc
     5161 agcgaagagt tcgtcacctc gctgggtatt caatggaacc cgtacactac ccagattgaa
     5221 ccgcacgact acattgccga actgtttgat tgcgttgcgc gcttcaacac cattctgatc
     5281 gactttgacc gtgacgtctg gggttatatc gcccttaacc acttcaaaca gaaaaccatt
     5341 gctggtgaga ttggttcttc caccatgccg cataaagtta acccgatcga cttcgaaaac
     5401 tccgaaggga atctgggcct ttccaacgcg gtattgcagc atctggcaag caaactgccg
     5461 gtttcccgct ggcagcgtga cctgaccgac tccaccgtgc tgcgtaacct cggcgtgggt
     5521 atcggttatg cgctgattgc gtatcaatcc accctgaaag gcgtgagcaa actggaagtg
     5581 aaccgtgacc atctgctgga tgagctggat cacaactggg aagtgctggc agaaccaatc
     5641 cagacagtta tgcgtcgcta tggcatcgaa aaaccgtacg agaagctgaa agagcttact
     5701 cgcggtaagc gcgttgacgc cgaaggcatg aagcagttta tcgatggtct ggcgttgcca
     5761 gaagaagaga aagcccgcct gaaagcgatg acgccggcta actatattgg tcgagctatc
     5821 acgatggttg atgagctgaa ataagcctca aatcagtgcc ggatggcgat gctgtccggc
     5881 ctgcttatta agattatcca ctttttcttt tttcacttta cctcccctcc ccgctggttt
     5941 atttaatgtt tacccccata aacacataat cgcgttacac tattttaata attaagacag
     6001 ggagaaataa aaatgcgcgt actggttgtt gaagacaatg cgttgttacg tcaccacctt
     6061 aaagttcaga ttcaggatgc tggtcatcag gtcgatgacg cagaagatgc caaagaagcc
     6121 gattattatc tcaatgaaca tttaccggat attgcgattg tcgatctcgg attgcctgac
     6181 gaggacggtc tgtcactgat tcgccgctgg cgtagcaacg atgtttcact gccgattctg
     6241 gtattaaccg cccgtgaaag ctggcaggac aaagtcgaag tattaagtgc cggtgctgat
     6301 gattacgtga ctaaaccgtt tcatattgaa gaggtgatgg cgcgaatgca ggcattaatg
     6361 cggcgtaata gcggtctggc ttcacaggtc atttcgctcc ccccgtttca ggttgatctc
     6421 tctcgccgtg aattatctat taatgacgaa gtgatcaaac tgaccgcgtt cgaatacacc
     6481 attatggaaa cgttgatacg caataatggc aaagtggtca gcaaagattc gttaatgcta
     6541 caactctatc cggatgcgga gctgcgggaa agccatacca ttgatgtact gatgggacgt
     6601 ctgcgcaaaa aaattcaggc acaatatccg caagaagtca ttaccaccgt tcgcggccag
     6661 ggctacctgt tcgaattgcg ctgatgaaaa aattactgcg tctttttttc ccgctctcgc
     6721 tgcgggtacg ttttctgttg gcaacggcag cggtagtact ggtgctttcg cttgcctacg
     6781 gaatggtcgc gctgatcggt tatagcgtca gtttcgataa aactacgttt cggctgttac
     6841 gtggcgagag caatctgttc tatacccttg cgcagtggga aaacaataag ttgcatgtcg
     6901 agttacccga aaatatcgac aagcaaagcc ccaccatgac gctaatttat gatgagaacg
     6961 ggcagctttt atgggcgcaa cgtgacgtgc cctggctgat gaaaatgatc cagcctgact
     7021 ggctgaaatc gaatggtttt catgaaattg aagcggatgt taacgatacc agcctcttgc
     7081 tgagtggaga tcattcgata cagcaacagt tgcaggaagt gcgggaagat gatgacgacg
     7141 cggagatgac ccactcggtg gcggtaaacg tctacccggc aacatcgcgg atgccaaaat
     7201 taaccattgt ggtggtggat accattccgg tggagctaaa aagttcctat atggtctgga
     7261 gctggtttat ctatgtgctc tcagccaatc tgctgttagt gatcccgctg ctgtgggtcg
     7321 ccgcctggtg gagtttacgc cccatcgaag ccctggcaaa agaagtccgc gaactggaag
     7381 aacataaccg cgaattgctc aatccagcca caacgcgaga actgaccagt ctggtacgaa
     7441 acctgaaccg attgttaaaa agtgaacgcg aacgttacga caaataccgt acgacgctca
     7501 ccgacctgac ccatagtctg aaaacgccac tggcggtgct gcaaagtacg ctgcgttctc
     7561 tgcgtagtga aaagatgagc gtcagtgatg ctgagccggt aatgctggag caaatcagcc
     7621 gcatttcaca gcaaattggc tactacctgc atcgtgccag tatgcgcggc gggacattgc
     7681 tcagccgcga gctgcatccg gtcgccccac tgctggacaa tctcacctca gcgctgaaca
     7741 aagtgtatca acgcaaaggg gtcaatatct ctctcgatat ttcgccagag atcagctttg
     7801 tcggtgagca gaacgatttt gtcgaggtga tgggcaacgt gctggataat gcctgtaaat
     7861 attgcctcga gtttgtcgaa atttctgcaa ggcaaaccga cgagcatctc tatattgtgg
     7921 tcgaggatga tggccccggt attccattaa gcaagcgaga ggtcattttc gaccgtggtc
     7981 aacgggttga tactttacgc cctgggcaag gtgtagggct ggcggtagcc cgcgaaatca
     8041 ccgagcaata tgagggtaaa atcgtcgccg gagagagcat gctgggcggt gcgcggatgg
     8101 aggtgatttt tggtcgccag cattctgcgc cgaaagatga ataaatatgt ccttttacca
     8161 ctacatcaag tcattgattc gatcaaacaa ttttcattga aaaagtgcca aaaatcgcaa
     8221 attgactaca ccattaacta caccgttcgg tgcactgtat gaaacaacgt ggaacaaata
     8281 gacacaagaa atatacaggc gggtaatctt tccaggggga agcgcaccaa ttcatgaggg
     8341 gcgttaatgt cgatatgggg atccccataa cggggctacc ggattttttt tccggttaac
     8401 ctttaatcag gctggtgggt tttacatacc cttgatcacc gtaatgatga tccagatgtg
     8461 caccttttcc accaatggcg gaaaagctcc aaccgtaacg atgatcgcta tggttgcctc
     8521 gatggtctgc gtggtcatta tgaccgttca gccagtgacc ttaatcagaa tttttcgcta
     8581 aaaaatgacg ttggccacgt catccggaaa gcacaaaatc acgtcgattt ttaatgaacg
     8641 gttaaaccgt ctttttgtca cggtgtgtca ctggttgtca ctctccgtca cgattgaggt
     8701 ggtggtcatt ctgcccgacc gcgcattttt gcgctatcgg gaatatcaaa cagttacctc
     8761 gccgacttcc tcaaattgag gtttccgaaa atatcagagc gggttagcga ttaagctgac
     8821 gagtaattaa actgcgaaaa tttcgtagtt tctgaatata ttccggcctg ctggttaaca
     8881 gtcctcagat gtgagggatg tagccagcgc aattttgcgc tttgctttaa tctcagtgag
     8941 ttaccgccgt aaccgctccg gcttcttcca gtggtacgtg attttctcct tctcccgata
     9001 cagttcaacg cggcgattgt aggccagcat ttccagaacg cggatccgta tgtcgcgcat
     9061 atccgcatca ttaagctgga taccatcacg gcgcatcacc tcagcaacaa cacgcgcata
     9121 attttcggct gtcacgctgt ccggctgcgt ggtctgttcg tcagcctgct ggctgattcc
     9181 agagacgcgg cggattaatc gcagtatttc ggcttctgtc atgctgctga cctcattaca
     9241 ccccgctaaa ttcttccagt ttctggcggt ggctgtcgct tatatcaaaa gcaaaatcct
     9301 catgctctgc ctggaatgta ccaaacgcca tcagcgccgc tacgctcggg tctatcttgt
     9361 tcggtgattt tttcttgttc ggcttgatat tggcgttcgc gtcactctgc atcacgacgt
     9421 tactcatcga ccaggacaac accggatcgc cacgatgcac aatcaccctg cggttaacaa
     9481 aaacttcgaa cgatttcgcc gccggactga atctgaggta ggtttgcggg aacggctcca
     9541 cctcaaaacc agccccctgt aattgcgtcc tgagatgcgt ggcgttccac gtatcaaagc
     9601 ccaccagcct gatattaaat ttctccgcat cctgcatgat gtcatctctg atccggtcgt
     9661 aatcaatgca gtcacctggc gttgtgcgta tccagcccgc tttagcccac tggcgataga
     9721 cagcgcggtt tttattggcg gggttctgta gctggaactc cggcagataa tgacgggaaa
     9781 tcagcataat ggttttaccg accggaaagg catagcatac gctggaaata tcgctggttg
     9841 atgataagtc cagccccgcg taacactcct gaccgtgtaa atcttcctcc gtgaacgttc
     9901 cggcacactc agcccatgca ccattaccca tccacggggt agtcccctga caccagatat
     9961 taaatcgctt tgtcatcatc tccacccact gcgacggaat accccgcgct ttctggatgg
    10021 ttgaggccag tttttcacga tccacggaaa catcaatatt agggttcgcc tttatccaca
    10081 tcgccggatc atcaacctcg ttttcgtcgt ccagttcgta aatcagtaca aaaattgaat
    10141 cgttgacctc ttcgccgtcc aggatctggc agcaataatc atagtgctgt ttacaggctg
    10201 aaacgacgtt actgcccgat gtggtaatgg caaataacag cccttcggga cgtgcgccca
    10261 tccccagctc aagcgcggaa taaacgccgt tatcggggtg taggtggtat tcgtccacga
    10321 tggaaagact ggggtttgtc ccctcgatgg ttgccgcttt tgctgccagc ggctttaaca
    10381 ggctgttgct tttcgggtgt atcaccttat gcgcctgaat attcacccgc ctgcgtaacg
    10441 gtcgggataa aaggcacatc tgacgcgcat tatcaaacac gatccgcgcc tggtcacggc
    10501 tcaccgctgc ggtgtagata tcctgctgac cgttctccat aatcagaaac cagttagcca
    10561 gaatcgcggc ggtcgtggat ttcgcatttt tgcgcggcac ttcgataaag gcgctcgtgt
    10621 atttgcgccg tccggtggcc ttaaccttaa agccgaggat gcacgcaaag gcgaactgct
    10681 gccacggctc cagctcaatg ggtctgccac gcatcggtcc ttttacgtgc gggcacaccc
    10741 tggaaaaggc aataaaccgc tccacaacct cacgatcgaa cgtgtaaagg gggcttttaa
    10801 ggtccgaaaa gtaccgttta acagcctgtt ttaaccgttt acaggccgga attttgcccg
    10861 tttttacgtc ttctgcgtac ttattccagg cggtcaagct cgtcctcttc ttctgtttcc
    10921 ggtggatttt tacggcggct tatcgggtca aaaccgagca aggaggccat ttttatcatc
    10981 actctttcag cgtcggattt tgcgcttaat gcggggtttc tgctctcgcc gccctgactg
    11041 ttaacaatgc tgaacccgcg cgccgcaagg tctgcaacgg ctttccggta aatggagtag
    11101 ttgacgcaat acagttccag attgctccag tcggcggggg tcaggtctcc ccgttccgca
    11161 agctgccgcg atttttcccg ccactgcttc acggcgatat catccaggta ggcggggtcg
    11221 ggtttgtcct gaaaacgccc cccaccccgt catacagcct catcagcgat tgcggaaaca
    11281 ttccataacc tcgcggtcac ggtcggttaa tcgcttcgct gtgatgcgtt ctgcgcgtcc
    11341 tgacgcttta tctttatgcc ctgtttcctg tgtcttccat gcgtcacgct gccttataag
    11401 tccacggata aggcggtttt gttcccgctc attcatcagc gtcatacatc cagttattgc
    11461 ggttagcagc ccgttcttcc tcttcacgaa atccacctgc ggcacgcttg ctttttgttg
    11521 ccggatcaag ccatttcgtt ttctggttat gacacgcctg gcacaatggt tgatggttcc
    11581 attcgggcca gaagagaaca tcatcaccgc cattaatcgg gataatgtga tccaccacca
    11641 cggcgggcgt atatatcccc ttctcaaggc atcgcacgca taacgggttt ttactcagat
    11701 acatggcgcg gtatttgtcc cactgtctgg aatatccacg cgcgcggcgg tgtcctcgtc
    11761 tggcatcctc tgcacgccat gccgcccgcc tgtgctcctc acacttgccg gatttaacgc
    11821 gcctgttaca gcctggttct gtgcatcgtc ttaatggttg ccacggcatc agtacccccc
    11881 cacatcacgg taagccgtcc agagtgcgcc tatcgtcatg ggtacgcgtg tttttgcgtt
    11941 atccgcgaca atctggcgat tttcatacag gtgagcgata aacatcatgc agccaatctt
    12001 tatggctggc gtgaacgcca gcccgtcatc aaagcgcctg cctatgtgta tctggcacgc
    12061 ctcaagcgat gcggcaatgt atccgctgat taactggtct tcatcgtcgc catcgatgcg
    12121 gcaatggagt ttcacttctt ccagggtgat aagttcttct gtcatttttc cgcgccctca
    12181 cgacaaagaa tttcaaggcg tgtcctggcg gcatccggca gcggctgccc gatgatattc
    12241 agtacacgcc ccgccagcgg ccccgtactg acctttatcc ggctggtggc gttgatgtcc
    12301 ttccggtacc gtatccagat ccttacggta ccaatcgcca gctctgcgcc cgatgaaatg
    12361 gcctccttgc tgctgaccat gttcacgctt gcccagagtg tgtgaccgtc ctcccacgtt
    12421 tcgagtattt cgccggttat atccctggtc tgtttcaggg tctgaatcgt tatcctgtca
    12481 cgcagtcgcc ctatgttcat tccggttttt ctccctcgct gatttttact tcctgtttcc
    12541 atgcctggct gaactcatca ccaccatcac gcggtgacag cccttcacgt tcgcgggctt
    12601 cgttcggaca cataacgtcg gattttatgc cgcgctcata ggtggcaaag cgttcgccag
    12661 gtgtggcccg taacaggtcc gcgctgtcaa actccacctg ataccggata ccaggtacag
    12721 gcgaggccac cagcaaggcg ttttttatct gctgctcaaa gttcgccagc cacgggcgca
    12781 tcgtcatggt gagaaatgcg cggcttgcct cgctgaagtt gctgtaggtg ctgttgctgt
    12841 attcctgcaa aaaaatcggc gagacgttga acattcgggc gatgtcttca atggtgaagc
    12901 gacgggaggc cagccattcg gcatcctgat tactcatgcc cagctgctga tagctcatcc
    12961 ccccttcaag gatgggcgtt tttccggcgt ttctggcccc tttgtagcgt tccagtgcgg
    13021 ctaatgcctg tttgcccttc acgccgtcca gccattcgcc tgacgtgata acccctgccg
    13081 ccatcatgcc atcacgcatc acgctcgcgc cgtggcgttg ttgggccagc ccaagcccca
    13141 gcgattcgcg gcagatggtt acaggtgagc gccccataaa accgtcatcc gtggagtaac
    13201 gaaggtggag aacctcccac ggtaaatagt tgcgggtgtt tccggtgtag gcgtcagtga
    13261 tgcagtagcg ccagttatga tcacctgtct gatccacgtt caccgactgc ggcgggtaag
    13321 gatgtaaagc cgccggaaaa ccatcacgcc cccactgaat aaccgcataa gcattaccac
    13381 ttaacaggca gtagcggatc atcattcgct taaactggta gggtgtctgc cacgcgttcg
    13441 ggcgctcgtt gaggatgtga tcgaccggat gagaatcaag ccattcgcgg gcctccttcc
    13501 ccttctcatt gcgtaccagg tacaggtaac acggcatggt agccaccgcg tcagagatga
    13561 ccgtgacggc gttcatgacg gcggggagtg attccgctgt cccagatgat acgtactcac
    13621 ccgcccccgt gttcgatgtg ccggccagcg ccataaattc atcaagcgtc atgctgcgct
    13681 gctctttttt tcgtctgaaa ggccacatat cacacccccg ctaaatccgc ccaccagcgg
    13741 cgattatccg cacgcggcat tttttcgggg tgctgctcat acagggaacg gcgggccagc
    13801 tccacgccgg aatcggggta agccggtacc gatgtaacgg taatttcgta cagttccgcc
    13861 accagcacgg tgcgcacgca tggatctgtt gtggtatccc atacatcctt acgggagcga
    13921 aagccaaagc tcatccccga gatgtcccca cgcttaacca gttcgataac gtcgcgccct
    13981 gtactgggat ccggtggggt cagttcaaag cgtaaccctg tttcgtcctc ttccagtttc
    14041 agcgtgccgg aacgggtgcg ccccagtaac atgctgtggt catgctcata caggccgcga
    14101 acgtcattac ccgccgcaag ccattcagta aacgcccccc gctggaattt ttcgtaaaac
    14161 tcaccccata acagttctga aagcttatcc cagcgaacaa cgtagcccgt cagcgtaccg
    14221 gctccgctgg tggtgatttc cgatgaccgg atttccatac tcttcataat tttttcaccc
    14281 ataaaacact aaaggggctt tttagcccct tctgtatgct gttaatcgtc gtcttccggc
    14341 agtgccagaa tcttgatcgc gttcgaatcc accacgccac cgcctaaata tttttgcgtg
    14401 aaaattttga tgaatcctgg ttctgtcagg ttgtccggtc tggtgcgaac acctgtttcg
    14461 tgatcaacaa tgtagtaacc gcgtttgaag tcacccaggg caataacgtt atcaggcata
    14521 aactccagat attcgaccgg aaggcccagc aacgtatcag gatcacccgc ctgtaaacgg
    14581 tcgcgccaga tataatcacc gttcgcgttc ttcaccttct gaagttttgc cgccgtcgtg
    14641 gagttaacca ctcagaccgc gtttttgcgg tattttttac gtaatgcaaa tttcaggtcg
    14701 atcagcgggt ccgcagatgt ccacgccaga gattcggaag gtttaattac ctgcaacgta
    14761 ccaaaatcac gctctttgtc gttcttctct gcacggggta cggataaaaa accttttgct
    14821 tttttgtcac cgtcgcccac aaccagatcg ctttcttcgg tttccgtgaa ggtgtcgcca
    14881 atctcacccg tcagccatga aaggatgtcc acatcggaaa aatccacgat ttcctgtgtg
    14941 gtgcgcgggt acgcatagac cggatacagc ttaatgctca cctcgttaat ctgcggggtg
    15001 ctggtctgtt cgcgtgcctt accctcttca ccgtggttaa cggtcgcacc gccagcggaa
    15061 acaagctgct taaactcgtt gctgctgatt ttcttcacgg tacagatgcg acgcatggtg
    15121 gattcatccg ccagcattcg catgatttcg gtgttcagtt cagggataac ggtataacca
    15181 ccatcagcgg gaacgcctgt acttaatgcg cgggtttcac cggtcagaat gtagttgcgt
    15241 agttctgcgg ggtcagtggt ctggctgctt ttacctggct tgctgcgctc ttcgtctgca
    15301 atggcttcaa ggcgggaaat gtctttatcg agggattcag ctttagcgcg taattcgtca
    15361 aattttgtgc cctcagcatc gttaagactg cggttttctt tttcagcgtt ctccagcatg
    15421 tcgcgcatct gatttttaat ggcggttttc tgctggcgta attcgattat tctctgcata
    15481 aaaaaagtcc tggggttaag taaggaactc caggacgcgg caaacactca accgtttttc
    15541 ataaggaaat cagtaatcgc accgatcgtt ttcccgcctg gtaatgaata ttggcgagca
    15601 cattaacagg cgggaaagtg gccccagcgt cctggcacca cgggcgagaa taatcatgat
    15661 tcagttcggg taaaatatgc cgatccgctc agtgaacaac gtggaacaac cacgaacaaa
    15721 taatttacaa aaaataacaa aaagccggat tgctccggct gtatgtgctg tatgggtgct
    15781 cagtctctga tccctttcag aaattctatc aatgcatctg tctgttcagg atttactgcc
    15841 agcatttcac cggatagcgc gcaacgaaca aaaccatgat gatccttttc aatcagcgcc
    15901 cccgtttcca gaaatgcgcg gtaatcagtg atattcatcg tctccatgtt gtcagcatgg
    15961 tatttatcac gctgtttaag tatctcatca aatttcatcg gcattgcgtt ttcctctgtt
    16021 gtgtctgttt gtttcagata gtaactatgc cggaccgtga cgaaaagcca gtagtgctcc
    16081 ataccgtttc aaatgacgca aaaaaagccg gattgctccg gctgcgtggt gtggttctga
    16141 tattcctact ctgctaattt gctcagcccc atgtctgcgt aagtgcgatt tactgcattt
    16201 ctcaggtctg cgtagttctc cgttggcggc tccggtgtgc ccttgctgct ctgttttacc
    16261 agcgggtcgc ccaccagccg cccgtaagct gcaatttgtg ctgtcatatt gccacctcag
    16321 tgaagcgatc cggcattgtt ctttttcatc tcttccatca ttttttcagt gagcattacg
    16381 cactgaaacc ccatgttttt gatgtctacg ggtttcatgc ctttgtcagt ggatatcaca
    16441 ggcagaatat cctgatacac atcgcggtca gggtaactaa gcacacaaag cgcccatgat
    16501 gccacgggtg aggatccttc gtctgtttca acccaccagc ctggtgtagc tgcgcttatt
    16561 tgcagaatcg tgatccgtcc gttgtgcttg taaaattttt caccaaattt tggttcgctc
    16621 atgattccac ctctccggtt ttaacgttga ttgttgttac ctgttccgct tcggcaatct
    16681 cccgctctgt cagcgtggca aaatttgccg ccgtcgtggt catgaatgcg cttatcagtt
    16741 cgggatgtgc tttcgcgtat ccttcccccg cgtggcggtc tatcgttctg attgccacct
    16801 ttaaagcgtg ctctgtcatg tctaacgcgc gatatttcgg ttctgttctg tctctgcgtt
    16861 ttttgaatga tttattaaat ttccctgaag tgtgcatatt tatttttacc ccctcgttta
    16921 aaaagttttg agttgtgcct ccccttgtct accttatcta ccttagtggc cctcatgcca
    16981 gtaatggcgc tgctttcagc ggggtagagt gcttttatcc actatctacc ccgtgtctac
    17041 ctccctgtct gattcaggta aaatcaggta gagagggtag atagtgggta gacagtaaaa
    17101 aaaggctatc tacctaactt aatgcactga attaaatgta tttttcttta ctcaggtaga
    17161 cagggtagac agcaattaca aaaaattata aaaacgcgtc gcactcgtct gttgttattg
    17221 cgttagtctg cgttactccc ttaactttcc gcgtaatata ttcatgtccg taaactttcg
    17281 cggctggctt catagccttg ccaaagtcat ttacgtttag cggtttgctc ctgcctgcgt
    17341 acgccataaa cgccagatag acgcggtaaa ggctgtttct ggtcgtgtac ttcactgaat
    17401 cgccaccgcc gcccatcatc aggccgcgtg cttcctccag aaaattcagg aactggcaaa
    17461 actcaataac cggatccgtc tgttgcttta ttgccagagc ttcatcaccg tcacgctgtt
    17521 ccagtagtaa agcccgtgct ttctcagagt cggtaaagtt cgccagcaat cggcggataa
    17581 tgacagggat ttcagccgca atcttttccg gtagctccct gtctttttcg gcctcactaa
    17641 cgatattgtc gaaacggaaa atcacgcgac gacgtgccac acctccggcc cgttcggtga
    17701 atatcatcgg gttattgttg gtcgccagca ccaccgccct gattacagcc gtgaaacgct
    17761 tttcgtattt cgggttaatt tccacggggt caccgcccgt gattttcttg atgcccgttc
    17821 cttcgcctgt atatttcggc tggtcagcca ggacgataag acgactcccg acaacctgcg
    17881 cacgtccacc agcatcatca agcgatgtca tttcagcgct taccgtgttc tgtttccctg
    17941 ccagaaggat ggctatgtgt gtgaatgtac ttttaccgct cccgccgtct ccggtggcct
    18001 caataaacat ctgccagtcg taccggttcg ccataatcat gtacagcgcg gcacatatac
    18061 gcatcatctt gcgctggtct tttccggctg cgtgctcaag ccatttatga aagtttagcg
    18121 cgttatcgcg gatgttctcc cctggtgctg gtggcgtgta ctcaatgccg ttgtgcgtgg
    18181 tgatccagtt ctccggcgtg tgcggggaaa attcccccgt tttcaggtca agcgcaccat
    18241 tggcgaacgg cagcaaatcg ccggacggct cgcccattgg ttcggcaata acttttaacg
    18301 cttccacggc gttattgatt acgcgcttgc tgaaagtggc cctgtgctct gaatagatcg
    18361 ccaccatttc gcggctaagt tccattgtgc tgaccggaca ccataccccg ccgcgccata
    18421 cgtgaacgat ttcactttcc tgatgcacac aaacgccatc aaagcgctcg gcaagcagct
    18481 gcgcgcgctc actgtctgcc atttgtgcca gttgcgtatt tttctgctcc ggcatggtaa
    18541 gccctgcggc aatattttca cgctcagcat tcagatagcg ccgccagttt tcacacttct
    18601 ggacgtgcat tccttcgggg taaaaatttg cgtcctgaat atctgccgcc gccagcttct
    18661 gaccaatctt tttggtctcc accaaatcca gttctccggc ctggtacagc cttacgcgtt
    18721 tttttccatc cggaacaatt ttcagtgcat caagttcggc aagctgattt ggcccaagcc
    18781 acacaggcgg cacattatcg ccggatgcgg ggccatcctg ctcctgccat tgtttcgcgt
    18841 gcgaccaggc atcactaccc gcaaagataa ttacctctgt gtctttgtgt tttattccgc
    18901 gtggctgttt ttttacgttc ggtgccagtt tcatttttta cccctgaata cgttaagcat
    18961 cttttttatt tcctgaatat tggcgcgtgc tttctccctg ctggttggtt tactgcgggg
    19021 cgctgcctgt accagcgtga aatcacgccg gaattgataa acaggcatca cgcaatcata
    19081 ttcgtaacct tcacggcggt aggtgatgcg ccgttctgcc acgcctttaa tcgttaccat
    19141 gccgccgtac tggtcgcggt aaatatcacc gcgcgtaaat ttagggtgag tgttgccact
    19201 ggcagttaag ccagaatatt taagtttcat tatttttatt ctccggtgtg ttgttcttta
    19261 tatctgtcgt gcaatagatc tatttcttgc agttccatta ttacaggctc aagaagcgtt
    19321 attaatgccg tggcaattct tgatttttgt ttgtcgcgtt cattgtcgcc aagtgtttca
    19381 agccatatgc gcaatatttc cagcatgttt tcactgtgag aaagtgcaag aaatgcgcgg
    19441 tctattgttt cgtggtaaat atcacgcata ttaatccccg tccgtcgttt ttcttaaaat
    19501 aacctctgtc acgaaatcag cataatcggc ggcgatattc agtacatcaa gcccgttgat
    19561 ttatattctc tcgtggaaag taagaaaaaa gccgctctaa taagttctgg cattgacgaa
    19621 agcgcatcag ccgcatcatc aggaacgccg gaaaattcct gtttcaggga attaaaacga
    19681 tcatcacgca taaccccccc cattttcaca atcggcaaca agaatatttt tagcgtcatt
    19741 cagtgaccgc gttgcgatgt atcggataca ttccagggcg aactgtgtcc ggtctttctg
    19801 ttcttctccc tgcgcaagct ctgccgtgca ttcaatatca ataagcgcgt gcatcagcgt
    19861 agtgagtgcg gcggctgctg cgttcggtgt ggttttattg tgcataggtt tccccctgac
    19921 gaatacgggc ggcgaataca gcaacacaac cggacgggca acggctacgc gcttcgcgtt
    19981 ccgtccaagc ggttacgtgg atgatttggg attctccggc actcagtgcc agaaaacgcc
    20041 acacaaaggc cgtttgtgtg tgtacaaggt gtggtatatg atttacggca accataacgg
    20101 ctcctagttt acgttgttgg ttagaagccc cgttactgct cctacagtgc ggggctttgc
    20161 tttttgtgtt gattgtgtgt aatgtgtcac tacacataaa tacattacct gaggtgtaat
    20221 taacgtgtca acacacaaaa acgaaaggcg tggtaatcct ccgttccagt tccgtttaga
    20281 tcctgactta cgggaaaaga tggaaaaggc acaacaacag gacggcgatg attctcttgc
    20341 tgcctggata aagcgaataa tccgcaaaga attacagcaa cgcggcatag aacccaaagg
    20401 ataaaaacta tcagcgccgt ggtgtgaggt actacggcgc attgctttac aggcgcacac
    20461 aatgaccaac gaagaatcaa ccgaaaaaac atcacctgca cgaaaaagac gacgcagaaa
    20521 gaaagagcat gaatcagaac gattcgcgcc gtgttctttt gcccttgaga agtaccttaa
    20581 agaatactca tcaaaggaga gaacttcgca gttatggcaa cgcactgaac cagactgatt
    20641 gcattgccca ccagcctgat agcggctatc atccccgtgc ttatgtttgg gatcacacac
    20701 acaaacggcg cagcgggtta tctgttcaga aaggcggctc catttcgggg ccgctttttt
    20761 tatgcctgaa aaaccccaat tttgtggttt tccagtttca ccagggcgaa cgaatccccg
    20821 cccacgttct ggcgtatatt caatcttcat ggttatagct ctgtgttcag atgattgatg
    20881 tgtggcggct gcgtgccgcc agcgtgatta atgaactgcc ttgcagctat ccttccaggc
    20941 cagaacctcg gataaagacc agccaacgga acgaccgcca agtttacgac gtgatgggaa
    21001 ttgtccggcc ttttccaggc ggtagcggca tgagcggcta aggcctgtta gcttttcgca
    21061 ttctttttca cgtataaacc gatcagtgct taacactatt gccccctttc gtttcttaaa
    21121 gagttatttc gtgttctatt gcgttgggat gtgtctgatt gtgtcaggat gattcagagt
    21181 tggcaaatgt tgaggccgta tggtttacag aaagaggaat aatcaggata aaatccttta
    21241 aattcatgtt aatacaaagg cataaaatat tgtttcatgc cttttttctc gctatttaaa
    21301 gagtgattcg ctagtgtata aaaaatcagt aacacattaa aaatcagcta cttataaatc
    21361 tgcacacttt ttcacctctt gttcgtagtt gttccgcttt gttgctcatt gttgcacgtt
    21421 gtatctgttc gcatatccag tatgcgcata ctgaaaaaac acgaaaaaaa ttattttctt
    21481 ctggctactg gtagcgtggt tacgttttca tgtgttcccg ccagtatccc taaccgctcc
    21541 gtccacatat ccagcgcatt gcgtttagca tccagataac gggaatgatt ataaactcgc
    21601 tgcattcctg gcatctggtg gcctgtaagc tgctccacga cgtgaggatc cacgcctaaa
    21661 tcgttcagca tcgttgtaaa ggtgcgccgg atgtcatgta gtgaccagtg agggtgattt
    21721 agcctcctgt gcgctaatct gccgtactgc gacacgcttg tttcctgttt cacttccccc
    21781 agcaataagc ccgtgtgcct gttctgctcc accagctgcg tgacgaacgg cagtattgct
    21841 tccggtatgg gccggaatat tgccaccttc gttttgctgt gttctttcgg cacggtccag
    21901 agcatttcgg taaaatccca ctcgctgatc tccgataacc tcagttctac cgtccggcat
    21961 ccgaacacaa tcaggaggcg gattaacgcg atgtagtagg gggagaatat ttttttgtcc
    22021 agtgcctgca ataattcgcc cagttctttg gtgcttaaga cacgctcgct tatatccggt
    22081 tttttcccaa cgtccgccac actcatatca tcaagaacgt tgctgattgc atagcgccgc
    22141 ctccggcaga acttaagcgc ctgtttgcac gtctgtagca agaatccggc agtaacaggc
    22201 gttcgctttg ccacctggtc aaaacaggcc agccagtgcc gtagctcgca tttatccagc
    22261 ggcatagcac caatgtgctg tattacgtga ttattaaggc gctttttcag ggcggcataa
    22321 tccacgcggt tttcctttgc gtacgactca agccagtagg tgagcgcatc gccaaccgtt
    22381 accggcttta acgcttcctg tacggtgtaa ttaagctcat gacgtggatt tttcccctct
    22441 gccagccatg cgcgacactg ggcggctttt tccctggctg atttcaggtt cagatcagga
    22501 taatttccca gcttaatgcg ttccggtggt gccccccttc ccgttccggc cctgtaagtg
    22561 aaataccagg ttaaaaggcc actggttgaa tgcctgacgc tcaggtttcc accgtcatta
    22621 agaaaggctg ttttttgggc gggtgtgccg ttgattttcc tcagctgtgt atcgctcagt
    22681 ttgttaagtg ctctgctcat aattttgatt ccggtcatca caattaacta caccattaac
    22741 tacaccgatc gttgcacaaa gggctacaac gtgaaacaag ctggaacaag gaaaatcaca
    22801 aatctgttta taatcaaaaa cataatgaac aatctgaaac attatgaaac ggcaaaaaac
    22861 actaattgat aaaatatgtc catacttcac gcattacgtt aagcatccgt tataatcggt
    22921 tgcagatacc agcctgtgga tgcttaacat ggaataccaa ctcactctta actggcccga
    22981 ttttcttgaa cgtcactggc agaaacgccc ggtggtgtta aaacgcggct ttaataattt
    23041 tattgacccg atctctccag acgagttggc gggtctggcg atggaaagcg aagttgacag
    23101 tcgactggtc agtcaccagg atggcaaatg gcaggtcagc cacggcccgt tcgaaagcta
    23161 cgatcatctc ggtgaaacca actggtcatt actggtacag gcagtaaacc actggcatga
    23221 gccgaccgcc gcgctgatgc gaccgttccg tgaactaccg gactggcgta ttgatgatct
    23281 gatgatttct ttttctgtac ctggcggcgg cgtcggcccg catctcgatc agtacgacgt
    23341 gtttatcatt cagggtaccg gacgtcgtcg ctggcgagtg ggcgaaaagc tgcaaatgaa
    23401 acagcactgc ccacatccgg atctgttaca ggtcgatccg ttcgaagcca tcatcgatga
    23461 agagctggag cctggtgata ttctttatat tccgccagga ttcccgcatg aaggctacgc
    23521 gctggaaaat gcgatgaact attccgtggg ttttcgcgcg ccaaatacgc gggaattaat
    23581 tagcggattt gccgattatg tgctgcaacg tgaactgggc ggcaactact acagcgatcc
    23641 ggatgttcca cctcgtgctc atcctgcgga tgttctgccg caagagatgg ataaactgcg
    23701 tgagatgatg ctcgaattga tcaaccagcc ggaacacttt aagcaatggt ttggcgagtt
    23761 tatatcccag tcacgtcatg aactggatat cgcgccgccg gaaccgcctt atcagccaga
    23821 tgaaatctac gatgcgctga aacaaggtga tgtgctggtg cgcctgggtg gtctgcgcgt
    23881 attgcgcatt ggcgacgacg tgtatgccaa tggtgagaag atcgattccc cgcaccgtcc
    23941 ggcactggat gcactcgcca gcaacattgc gctgactgcg gagaattttg gcgatgcgct
    24001 ggaagatccg tcattcctcg cgatgctcgc ggcgctggtc aatagcgggt attggttctt
    24061 cgaagggtaa gtttggattt aagccggatg cagcatcaca tgccgcatcc cttttcgctt
    24121 acttccgttg cgccgttaac tcggcaatac ggacgatcac ctgcaccgct ttttccatac
    24181 cttccagaat cacaaactca tgcttaccat gatagttgta accgccagtg aacaggttcg
    24241 ggcaaggtaa tcccataaac gacaactgcg cgccgtcggt accgccgcgg atcggtttca
    24301 gttccggttc aatatcgcag tcacgcatcg cctgctgggc gatatcgaga atatgcggat
    24361 gctcaaccac tttctcgcgc atattgtagt aactgtcttc aatcaccaat tcaatgtagc
    24421 aatcaggatg taaccctttg cccacttttt tggcgatctc catcatttta cgtttacgcg
    24481 cttcaaactg tttacggtcg aaatcacgga tgatgtagtg catatcggcc cgttcaacgg
    24541 tgcctttcat gctcgccagg tgatagaaac cttcatagcc ttctgtcatt tccgggcttt
    24601 catccgccgg aacttccgca tgaatacgtg ccgccagcga cagcgcattt accatcactc
    24661 ctttcgccgt gcccggatga acattgttac cgacaatttt tatattgacc gatgcggcgt
    24721 tgaagttttc aaactccagt tcgcctacgc caccaccgtc aacagtgtaa gcccagcggg
    24781 catcgaaggc atcaacatca aaatgtttcg cccctttgcc cacttcttca tccggggtaa
    24841 aggcgacgcg aatatcacca tgcggaatgt ttttctgttg caataccgcc agcgcggtca
    24901 tgatttctgc aatacctgct ttgtcatcag cacctaacaa ggttttacca tcggtggtaa
    24961 tcaacgtctg acccagtagc tgatgcagca ccgggaacat aaccggtgat aaaacttcat
    25021 cgccgatacc cagcgcaata tcgccaccgc gatagttttc aacaatttgc ggattaacat
    25081 ttttgccgct gcaatccggt gaggtatcca catgagaaat aaagccaatc gccgggatat
    25141 cgccagggac gttagccggt aacgtcgcca tcaaagtgcc cttctcactt aaggtcacat
    25201 tgataagccc catttcttcg agctgctctt tcagcagatg caataacttc cattggcctt
    25261 ccgtgctggg aacctgtctc acccctgctt ttgattgggt atccagagac acgtagttca
    25321 aaaatcgctc aagtagttta tccatgtagt caccctcact ttttgtgaca acattattaa
    25381 gaagcaagaa aagacaaata ttgcgtcagg tcacttttac ccctgcaagc gggaatattt
    25441 atcagcatta ccttaatgaa tataaagcta agccagtaat tcacaacaaa gattggcgat
    25501 tcaagcggtt tgccgtagaa ttaccgccct cattaagagt caccaaggtg gttaaccaca
    25561 aaccccgcat cggtaagcca tccgttgcgt ttacatggga cagagtaaaa aattgaataa
    25621 acaaccgagt tcgctttcac cgctggtgca attggcggga attcgcaaat gctttgatgg
    25681 taaagaggtc attccccagc tggatctgac tatcaacaat ggcgagttcc tcacgctgct
    25741 tggcccttct ggctgcggta aaacaaccgt tcttcgcctg attgcaggtc tggaaactgt
    25801 tgattccgga cgcatcatgc tggataacga ggacatcacc cacgttccgg cggaaaaccg
    25861 ctatgtgaac actgttttcc aaagctatgc acttttcccc cacatgaccg tgttcgaaaa
    25921 tgtggccttt gggttgcgca tgcaaaaaac ccccgctgct gaaattacgc cccgcgtgat
    25981 ggaagccctg cggatggtgc agctggaaac cttcgctcaa cgtaaaccgc atcagctctc
    26041 tggtggtcaa cagcaacgcg tcgccattgc tcgcgcggtg gttaacaagc ctcgtctgtt
    26101 gttgctggat gagtcgctct cagcgctgga ttacaaactg cgtaagcaaa tgcagaacga
    26161 gctgaaagcg ttacagcgta agcttggcat tacattcgtt ttcgtgactc acgaccagga
    26221 agaagcactc accatgtcgg acaggattgt ggtgatgcgc gatggtcgca ttgagcaaga
    26281 cggcacgccg cgtgaaatct acgaagagcc gaaaaacctg tttgttgccg ggttcattgg
    26341 cgaaattaat atgtttaacg ccacggtgat cgagcgactc gacgagcagc gcgtacgcgc
    26401 caacgttgaa ggccgcgaat gtaatatcta cgttaacttc gccgttgaac cggggcaaaa
    26461 actgcatgtt ctgctgcgcc cggaagactt acgtgttgaa gagattaacg acgacaacca
    26521 cgctgaaggg ctgattggtt acgttcgtga gcgtaactac aaaggcatga cgctggagtc
    26581 ggttgttgaa ctggaaaatg gcaagatggt gatggtcagc gaattcttca atgaagacga
    26641 tcctgacttt gaccactctc tcgaccaaaa aatggccatt aattgggtag aaagctggga
    26701 ggtcgtactg gctgatgaag aacacaagta agttccagaa tgtagtgatt gtcactattg
    26761 tcggttggct tgtgttgttt gtctttctgc ccaacctgat gatcattggc accagctttt
    26821 tgacccgcga cgacgccagt ttcgtcaaaa tggtctttac gctggataac tacacgcgtc
    26881 tgctcgatcc gctctatttt gaagtgctat tgcactcgct gaatatggcg ctgatcgcca
    26941 ccctcgcctg cctggtgctg ggctatccat ttgcctggtt tctggcgaag ctgccacaca
    27001 aggtgcgtcc gctgctgctg tttctgctga ttgttccgtt ctggaccaac tcattgattc
    27061 gtatctacgg gctgaaaatt ttcctcagca ccaaaggcta tctcaacgag tttttgctct
    27121 ggctgggcgt tatcgacaca ccaatccgta tcatgttcac gcccagtgcg gtgattatcg
    27181 gtctggttta cattctgctg ccgtttatgg tgatgccgct gtactccagt atcgaaaaac
    27241 tggataagcc gttgcttgag gcggcgcgcg atctcggtgc cagcaagtta cagactttta
    27301 tccgcatcat tattccactg acgatgccgg ggattattgc cggatgtctg ctggtgatgc
    27361 tgccagcgat gggcctgttc tatgtgtccg acctgatggg cggtgcgaaa aacctgctga
    27421 tcggtaacgt catcaaggta cagttcctca atattcgtga ctggccgttt ggtgcagcca
    27481 ccagcattac gctgactatc gtaatgggcc tgatgttgct ggtttactgg cgcgcttctc
    27541 gtttgctgaa taagaaggtg gaactcgaat gatcggtcga ctgcttcgcg gcggttttat
    27601 gaccgctatc tacgcgtacc tgtatatccc aatcattatt ttgattgtga actcctttaa
    27661 cagctcgcgc tttggcatca actggcaggg ttttaccacc aaatggtata gcctgctgat
    27721 gaacaacgac agcctgttac aggcagcgca gcattcacta acaatggcgg tgttttcggc
    27781 gacgtttgct acgcttatcg gttcactgac ggcagttgcg ctgtaccgtt atcgctttcg
    27841 cggtaagccg ttcgttagcg gaatgctgtt tgtggtaatg atgtcaccag atatcgtgat
    27901 ggcaatttcg ctgctggtgc tgtttatgct gctgggtatt cagcttggct tctggtcgct
    27961 gctgttctcg catatcacct tctgcctgcc atttgtggtg gtgacggtgt attcgcgcct
    28021 gaaaggtttt gacgtgcgga tgctggaagc ggcgaaagat ctcggtgcca gcgaatttac
    28081 cattctgcga aaaatcattc tgccactggc aatgccagcg gtggcggcgg gctgggtgtt
    28141 aagctttacc ctgtcgatgg acgatgtggt tgtttcttca ttcgtcaccg gaccaagtta
    28201 tgaaattctg ccgttaaaaa tttattcgat ggtcaaagtc ggcgtatcgc cggaagttaa
    28261 cgcgctggca accatattac tggtgctgtc gctggtgatg gttattgcca gccagcttat
    28321 tgctcgtgat aaaacgaaag gtaacacagg ggacgttaaa tgaaaaaatg gtcacgccac
    28381 ctgctcgcgg cgggtgctct ggcactgggc atgagcgccg ctcacgccga tgacaacaac
    28441 acgctgtatt tctacaactg gaccgagtac gtgccgccag gactgcttga acagttcacc
    28501 aaagaaaccg gtattaaggt tatctattcg acttacgagt cgaacgaaac catgtacgcc
    28561 aagctgaaaa cgtacaaaga cggtgcctat gacctggtgg ttccttcaac ctattacgtc
    28621 gataaaatgc gtaaagaggg gatgatccag aagatcgaca agtcgaagtt aacgaacttc
    28681 agcaatctcg atccagacat gctcaacaag ccttttgacc cgaataacga ctactccatt
    28741 ccgtatatct ggggtgcgac ggcgattggt gttaacggtg atgcggtgga tccgaaatct
    28801 gtcaccagct gggccgatct gtggaagcca gagtacaaag gcagcctgct gttgaccgac
    28861 gatgcccgtg aagtgttcca gatggcgctg cgtaagctgg gctactccgg taacaccacc
    28921 gatccgaaag agattgaagc tgcatataac gagctgaaaa aactgatgcc aaacgtcgca
    28981 gcgtttaact ccgataaccc ggctaacccg tacatggaag gcgaagttaa cctcggcatg
    29041 atctggaacg gttctgcttt cgttgcacgc caggcgggta cgcctattga cgtggtgtgg
    29101 ccgaaagaag gcggcatatt ctggatggac agcctggcga tcccggcaaa tgccaaaaac
    29161 aaagaaggcg cgctgaaatt gatcaacttc ctgctgcgcc cggatgtggc aaaacaggtt
    29221 gctgaaacta tcggttatcc aacgccaaac cttgcggcgc gtaagctgtt aagtccagaa
    29281 gtggcaaacg ataagacgct ctacccggat gctgaaacca ttaaaaatgg cgaatggcag
    29341 aatgacgttg gcgcagccag cagcatttat gaagagtatt atcagaagct gaaagcagga
    29401 cgttaattca gcaagcaggc ggtggtatta ccgcccgttt tttagttagc aaggatgtgg
    29461 ctaatgagtc aggacagtaa agttttatcc cgctctttcg tcggtattgt ggtccgcttg
    29521 taggcctgat aagcgtagcg catcaggcaa tgcttcctgc tttataatcc ctccagcaac
    29581 ttttcaacaa attctggcac cacctggctt gccgggccgt aatatttctc ggcaaattca
    29641 ttaccaacct gactcggttc aagattcagt tcaacggtgt gagcaccatg tagtttggct
    29701 tcgtgtacaa acccagccgc cggatagaca tgcccggaag taccaatggc aataaagaca
    29761 tccgccatcg acaacgccat ataaatttca tccatgccga gtggcatttc gccaaaccaa
    29821 acgacgtgcg ggcgcaaggg tgccgggaac tggcaacaat ggcatttatc ttctggcgta
    29881 acgtctccgg tccagtcgag aacctgacca ctttgcgaac aacgaacttt cagcaattcc
    29941 ccatgcatat gaatgacatt ggtattacct gcgcgttcat gcaggttgtc tatattctgc
    30001 gttaccagca aaaagcgatc gccgagagcg tcttgcagtt tagccagcgc aagatgcgcg
    30061 gcgttaggct gaatttctgg ctgctgcagc tgtcgacgac gggcgttata aaacgcttgc
    30121 accagttcag gatcgcgatc gaaaccttcc ggcgttgcca catcttcaac ccgatgttct
    30181 tcccacaggc catctgcggc gcgaaaggta cgaatacctg attccgcaga aattcctgcc
    30241 cctgtcagta cgagtactct tggtttttcc attgcttccg gcaccacttt atctctgaaa
    30301 aaaatacgct gacgcaaacg ctcgcgcagg cgacgtttat ttttacgaaa acgacttaac
    30361 cgatgacccc gacgcgacag catagcaacc tctgttgttt aatcggttag atgtaggaag
    30421 gccgcaccac gcattcctcc cgcatcaccg tggcgcgcgc gttcaatgcg cggaacacga
    30481 gctacaggta agagatgaca aggcaacctg tccgccagtt gcgttgtgat tgccgggaaa
    30541 ttcgataagc caccaccaat gacgaccagg tcagggtcaa caatggtcag gatatttccc
    30601 agagaaaccg ctaataaatc cagataacgc tcaacgtgcg cccttgcctg ctcatcgcct
    30661 tgatcataaa gcgcaataat ttcgggagcc tgcaacggtt gatgataata gtgttgatac
    30721 agccacgcaa aaccgcgacc agacagataa ttttcaatgc agccatgctg accacagccg
    30781 cagcggcgta acgggaaatc caaccccatc atggttaacg catcaaccgg cagacgcata
    30841 tggccaaact cgccggtaat gtagctttta ccagtaatcg gtttgccgtt gaaaaccagc
    30901 ccgccgccaa cgccggtgcc gagaattaac cccatcacca gtggatattg cgtaaattca
    30961 tcatcccagg cttctgaaag ggcaaaacag ttggcatcgt tatcaaggcg tacatcgcga
    31021 tcaagacgtg cgctcaggtc ggcacgcagc ggtttgccgc tggcagcggg gacattagcg
    31081 gcatacagct taccatcttc tgtttccggc attcccggaa taccgatgcc gacagaacct
    31141 ttacagccaa aacgttggtc agcttcagct accagctcac acactgcatc taaaaatgcg
    31201 tcatagctgt cacgcggtgt cggcacccgc ttttcccact gcaactgccg accgctatca
    31261 aacacgccaa gcgcaatttt tgttccacca atatcaaacc cgtaatacat tgccgctcct
    31321 tgttttaatg tactgccttt actggccgct aaggactcgc gcagggtcaa tattactggc
    31381 gcgccgcgcc ggataccaac ttgccaaaag actcagcaac aatgctgtga ccagtacgta
    31441 gaagacgtcc agccagtgca attccgatgg caggaagtca ataaaataaa tatcgctgga
    31501 gaggaactga tgaccgatca gcttttcaat ccactcaata atcggggtaa gttgcagtga
    31561 aacaactacg ccgataacca ccccacacag gctgccgaac agccctgcca gcaatccata
    31621 ccagacaaag atggcgcgaa ttaaaccatc tttcgccccc agcgttctta atactgcgat
    31681 atcgccactc ttgtctttca ccgccatcac taaggtggag acgatgttga aacaggccac
    31741 gccaatcacc agtaccatcg ccagatacat aatggcgcgg atcatctgga tatcgcgata
    31801 catatagccg taagtaccaa tccaactttt aatataaaca tagctgttgg tcacttcacc
    31861 cgcatcgcgt accagcttat tggcgttgaa aacatccgtc attttaaggg caatacctga
    31921 cacgctggaa cccatatcaa gatattgttg ggcatccgcc agcgggatca tggcaaaact
    31981 gtgatcgagt tgaccactca actgcaaaat accggcaatg tgcaaacgca cacgttttgg
    32041 ctgcatcagt ttatgctcgg gattcgagtt ggggatcatg atcgacaccc aatcgccctg
    32101 cttcactttc agcgcatccg ccacgccttt gccgatgata atttgctgtt cgcccgcttt
    32161 aaaattgcgc cacgcatccc cctgaacaaa cgagggtaat gcgctcagac gctgttcctg
    32221 ttgtgggtta acgcccttca cctggattgc gcgcagattc gctccacttt ccaccagacc
    32281 ggtgaaattg atatacggcg cagcggcggc aatacccggc actttctgca cgttatccag
    32341 tgcttcctgc cagttagtcc atggctgatt taccgcttcg atttcgccat gcggcaccac
    32401 cgccagaatg cggttattca gttcgcgttc aaagccgttc atcgcgctta agccgacgat
    32461 caataccgcc acgccgaggg caatgccaat ggtagaaatg acagagatta gcgacaccat
    32521 gccgccgcgt cgccgtccgc gactaaaacg caggccaatc aataacgata aaggcatcgc
    32581 cattactccg cccccatcag gctcagttcc gccgtcagac gtccatcacg catttccagt
    32641 tggcggctca tacgtttcgc cagttgcagg tcgtgagtga ccaccaggaa agcggtgccc
    32701 tgcaagcgat tcaactcccc aagcaactgg aagatgctgt cggcgttacg cgcatcgagg
    32761 ttaccggtag gttcatccgc cagtaccaga cgcggattat tgaccagcgc acgggcaatc
    32821 gctacacgct ggcgttcgcc gccagaaagt tcagatgggc ggtgattcgc acgatgctcc
    32881 agccccaccg cttttaacat ctccagtgca cagctgttga tttcagcggg ctttttcttg
    32941 ccaatcagca gtggcatagc cacgttttcc agggcagtaa aatccggcag caggtggtga
    33001 aactgataaa taaagcccag cttctggttg cgcagttctg ctttcgccgc tgaagacagt
    33061 ttgctcatcg gctgaccgtt aaagatcaca tcgccggagg ttggcgtatc cagcccgcct
    33121 aacaggtgca gcaaggtact tttaccggaa ccagagctac cgacaattgc catcatttca
    33181 ccttcgccca cgctgaaact gacattgagc agcacatcgg tttgcacact gccttcctga
    33241 tagcgtttgc acaggttgtc gcattgcaac aggatcttat tcataacgta aagcctcagc
    33301 gggttgtgtg gcggcagcgc gccatgaagg gtaaagcgta gacagcagcg cgatagccat
    33361 cgccaccagc gcaataacaa tgacctgtaa aggttcgata gccaccggca gcgccgcgcc
    33421 atcaagcagg acgccgatta tcggcatcag attatttaac tggctggcaa gcagtgcgcc
    33481 aagcgccgct ccgaggattg caccgataat cccggcgcta gccccttgca ccataaagac
    33541 catcatgatt tgtcgcggag ttaagccttg cgtttgcagg atcgcgactt cgccctgctt
    33601 ctccatcacc atcagcccca gcgaggtaat aatgttaaac gccgcaacag cgacaatcag
    33661 gctcagcagt aagcccatca tatttttttc catgcgtacg gcctggaaca gctcgccttt
    33721 acggtcacgc cagtcctgcc atttgctgcc ttcaggcagt ttttgctgac ttaaagagtc
    33781 aactctcagc ggctcatcca gccacaaacg ccagccggta atattggctg ccggataacg
    33841 catcagacgc gaggcatcct caatattcac cagcatttca tagccatcga cttcactgtt
    33901 ggcggcgaaa gtgccaatca cattgaacag gcgctgactt ggaatacgcc ccatcggcgt
    33961 gaactggctg gcagatggca ccatcacgcg gatttgatca ccgcgattga cgcccaactg
    34021 tgaggcaagt tgttcgccga ggatgacatt atatttcccc ggctcgaggt cagtttgttt
    34081 cacattgacc agatacggcg ttagtggatc tttttgcgcc gggtcgatac cgagcatcac
    34141 cccgaccgcc acgctgcgcg cgctttgcag taccacatca ccggtagtaa taggtgcgac
    34201 gcgattaacg ccgtccagtt tgactgccgt ttccgggagt tgctgcgggt taagagagcc
    34261 atgctcagaa gagagaattg cctgtggcat caggccaagg atgttgtttt gcagctcgcg
    34321 ctcaaagccg ttcatcactg acaatactgt gaccagcgcc atcaccccga gggtaatgcc
    34381 gatggtagaa agccaggaga cgaaacgacc gaagcgatcc actgcacgcc cacgcatgta
    34441 acgcaggcca ataaatagag cgacaggttg gtacatgaaa tccgtctggt tgctgtagca
    34501 aagacacgag tatataagcg aaagcaagat gggtaaacgt ctgaactgta agtgatcgtc
    34561 agggctaatc tgaaaagccc ccgtaaatca atcggctaaa ttgccatgtt ttatcatcaa
    34621 tcttgccgcc tgatcttaat ctgaaaaatc atccaataca gacagatacc cattacacct
    34681 cgggtcataa ccagcaggat caaccgcatg taaattaaaa aagagcgtgt tgttctgatg
    34741 aaacaaaaag agctatggat taaccagatc aaagggttat gtatttgtct ggtggtgatt
    34801 tatcactcgg tcattacctt ttatccgcat atgaccactt ttcagcatcc gttatcggaa
    34861 gtcctgagca aatgctggat ctatttcaat ctttaccttg ccccctttcg tatgccggtt
    34921 tttttcttta tatccggcta tttgattcgc cgctatatcg acagcgtacc gtggggaaat
    34981 tgtctcgata aacggatctg gaacatcttc tgggtgctgg cgctttgggg cgtggtgcag
    35041 tggctggcgt taagtgcgct gaaccagtgg ctggcacctg agcgcgattt aagcaatgcc
    35101 tccaatgccg cttatgccga ttccaccggt gagttcctgc acgggatgat caccgccagc
    35161 accagcttgt ggtatctgta tgcgttaatt gtctatttcg tggtatgtaa aatttttagc
    35221 cgtctggcgc tgccactatt cgccttgttt gtactgctga gtgtggcggt taatttcgtt
    35281 cccacgccgt ggtggggaat gaacagtgtg atacgcaatt tgccttatta cagccttggc
    35341 gcatggtttg gcgcaacaat aatgacctgt gttaaagagg tgccgttgcg ccgccatctg
    35401 ctgatggctt ctttgcttac cgttctggcg gtcggtgcct ggttgtttac tatctcgctg
    35461 ctgttgtcgc tggtatcgat tgtggtgatc atgaagctgt tttatcagta cgagcaacga
    35521 ttcggtatgc gctccaccag cctgctgaat gtgattggct ccaacaccat tgctatctac
    35581 accacccatc gcattctggt tgaaatattc agcttaactc tgcttgcgca aatgaacgca
    35641 gcacgctggt cgccgcaagt cgaactgaca ctcctgctgg tttatccctt tgtcagtttg
    35701 ttcatctata ctgttgcggg cttgctggta agaaaacttt cacagcgtgc attcagcgat
    35761 ctgttgttct ccccgccttc actgcccgct gccgtcagtt actctcgtta aaccatcacc
    35821 ccagccgggg cgattcaatt tgctaaacca tgtcgattac ggataatgaa cagcattgct
    35881 tatcagagat atgcccccat atgttgaggc atatcctaac gagaatctga caaccgttat
    35941 gcctgaacaa tatcgttata cgctgcccgt caaagcgggt gagcagcgtc tgctgggcga
    36001 gttaaccggc gcagcctgtg caacgctggt agcggaaatt gccgaacgtc acgccggtcc
    36061 ggtggtactc attgcaccag atatgcaaaa tgctctgcgt ttgcatgatg aaatcagcca
    36121 gttcaccgat cagatggtga tgaatctggc ggactgggaa actcttccct acgacagttt
    36181 ttcgcctcat caggacatta tctcctcgcg cctttccacc ctttaccagc taccgacgat
    36241 gcagcgtggc atactgattg ttccggtgaa tacgcttatg cagcgcgttt gcccacacag
    36301 ttttctccac ggtcatgcgc tggtgatgaa aaaaggtcag cgcctgtcac gagatgcatt
    36361 acgaacccaa ctggacagcg ccggttatcg ccatgttgac caggtgatgg agcacggcga
    36421 atacgccacg cgcggcgcgt tgctggatct cttcccgatg gggagtgagc tgccttatcg
    36481 tcttgatttc tttgatgatg aaatcgacag cctgcgggtg tttgacgtcg acagccagcg
    36541 cacgctggag gaagtagaag cgatcaatct gctgcccgcg cacgaatttc cgaccgataa
    36601 agcggcaatt gaactgttcc gcagccagtg gcgcgatacc ttcgaagtga agcgcgatcc
    36661 ggaacatatt taccagcaag tgagtaaagg cacattacct gctgggatcg agtactggca
    36721 gccattgttc ttcagcgaac cactgccgcc gctgttcagt tatttccctg ccaatacctt
    36781 gctcgtgaat actggcgatc tggaaaccag tgccgaacgt ttccaggctg acacgctggc
    36841 gcgttttgag aatcgcggcg tcgatccgat gcgcccgctg ttgccaccac aatcgctctg
    36901 gctgcgggtg gacgagctct tctcagagct gaaaaactgg ccccgagtgc agctaaaaac
    36961 tgaacattta ccgacaaaag ccgcgaatgc caatttaggt ttccagaaac tgccagacct
    37021 ggccgttcag gcgcaacaaa aagcgccgct ggatgcgctg cgtaagttcc tcgagacttt
    37081 cgacggtccg gtggtgttct cggtagaaag tgaaggtcgc cgtgaagcgc tgggtgaact
    37141 gctcgcacga attaaaattg ctccgcaacg cattatgcgt cttgatgaag ccagcgaccg
    37201 tgggcgttat ctgatgattg gcgctgccga acatggtttt gtcgataagg cgcgtaatct
    37261 ggcgctgatc tgcgaaagcg atctgctcgg tgaacgtgtt gcccgccgtc gtcaggattc
    37321 tcgccgcacc atcaaccccg atacactgat ccgtaacctt gcggagctgc atattggtca
    37381 gccggtggtc catctggagc acggcgtcgg tcgctatgcc ggaatgacca cgctggaagc
    37441 tggcggcatt actggcgagt acctgatgct cacctatgcc aacgacgcca aactgtatgt
    37501 tccggtgtcg tcactgcatc tgattagccg ttacgcaggt ggcgcggaag aaaacgcccc
    37561 gctgcataaa cttggcggcg atgcgtggtc acgcgcgcgg cagaaagcgg cggaaaaagt
    37621 gcgtgatgtg gcggcggaat tgctggatat ctacgcgcaa cgcgccgcca aagagggctt
    37681 cgcgtttaaa cacgatcgtg agcagtatca gttattctgc gacagcttcc cgtttgaaac
    37741 cacgccggat caggcgcagg ccattaatgc ggtacttagc gacatgtgtc agccgctggc
    37801 aatggatcgt ctggtgtgcg gcgatgttgg ctttggtaaa acagaagtgg cgatgcgcgc
    37861 cgctttcctg gcagtagata accacaagca ggtagcggtg ttggtgccta ccacccttct
    37921 tgcgcagcag cattacgaca acttccgcga ccgtttcgcc aactggccgg tacgtatcga
    37981 aatgatctcc cgtttccgca gcgccaaaga gcagacgcaa atccttgcgg aagtggcgga
    38041 agggaaaatc gatattctga tcggtacgca caaactgctg caaagtgacg tcaagtttaa
    38101 agatttaggc ctgctgattg tcgatgaaga acaccgcttc ggggtgcgtc ataaagagcg
    38161 cattaaagcg atgcgcgcga acgtggatat tctgacgctt actgcaacgc cgatcccacg
    38221 tacgctgaat atggcaatga gcggaatgcg tgacctgtcg attatcgcca cgccgcccgc
    38281 ccgtcgtctg gcagttaaaa cctttgtccg tgagtatgac agcctggtgg tccgggaggc
    38341 gatcctgcgt gaaattttgc gcggaggaca ggtttattat ctctacaatg atgtggaaaa
    38401 cattcagaaa gccgccgaac ggctggcaga actggtgcct gaagcacgga ttgccatcgg
    38461 tcacgggcag atgcgcgagc gcgaactgga acgggtgatg aatgatttcc atcatcaacg
    38521 tttcaacgtg ctggtttgta ccaccattat cgaaaccggg atcgacatcc cgacagccaa
    38581 caccattatc attgaacgtg cggatcactt cggtctggcg cagctgcacc agttacgcgg
    38641 tcgcgtcgga cgttcacatc atcaggcata tgcatggctg ctgacgccgc atccaaaagc
    38701 gatgactacc gatgcacaaa aacgtcttga agcgattgcc tcgctggaag atctcggggc
    38761 aggttttgcg ctggcaacgc acgatctgga gattcgcggc gcgggtgaac tgcttggcga
    38821 agaacaaagc ggctcaatgg aaaccatcgg tttctcgctg tatatggagt tgctggaaaa
    38881 cgccgtcgat gcactgaaag ccggacgcga gccgtcgctg gaagatctca ccagccagca
    38941 aacagaagtc gagctgcgga tgccgtcgct attgccagat gatttcatcc ctgacgtgaa
    39001 cacgcgtctg tcgttctaca aacgtattgc cagcgccaaa acggaaaacg aactggaaga
    39061 gatcaaagtc gagcttatcg atcgcttcgg cctgctgcca gatccggcgc gtaccctgct
    39121 ggatattgcc cgtctgcgcc agcaagcgca gaaactgggg atcaggaagc tggaaggtaa
    39181 tgagaaaggc ggggtgatcg aatttgccga gaagaatcac gttaatccgg cctggttgat
    39241 tggtttgctg caaaaacagc cgcagcatta ccgccttgat ggtccgacgc gcctgaaatt
    39301 tattcaggat ttgagtgagc ggaaaacgcg tatcgaatgg gtacgccagt ttatgcgtga
    39361 actggaagag aacgcgatcg cttaatgtaa atcgggggcg tttctgcgcc cccatctgca
    39421 acatttacaa attctttgca ctttcctgca ctatccgaca ctgtcaccat ccataattca
    39481 ggcttatctg tttattacaa taaccttata tttattatgt atttttggtg atgatcaaaa
    39541 cgcgtttttc tcgctggcta acgtttttta cgttcgccgc tgccgtggcg ctggcgctac
    39601 cggcaaaagc caacacctgg ccgctgccgc cagcgagcag tcgtctggtt ggcgaaaaca
    39661 aatttcatgt ggtggaaaat gacggtggtt ctctggaagc catcgccaaa aaatataacg
    39721 tcggctttct cgctctgtta caagctaacc ccggcgttga tccttacgta ccgcgcgcgg
    39781 gcagcgtgtt aacgatcccg ttgcaaaccc tacttccaga cgcgccgcgc gaaggcattg
    39841 tgatcaacat tgcggagctg cgtctctatt actacccgcc gggtaaaaat tcggtaaccg
    39901 tgtatccaat aggtattggt cagttaggtg gtgacacgct gacaccgaca atggtgacca
    39961 ccgtttcaga caaacgtgca aacccaacct ggacgccaac ggcaaacatc cgcgcccgtt
    40021 ataaagcaca gggaattgag ttgcctgcgg tagtgccggc tggaccggat aacccaatgg
    40081 gccatcatgc gattcgtctg gcggcctatg gcggcgttta tttgcttcac ggtacgaacg
    40141 ccgatttcgg cattggcatg cgggtaagtt ctggctgtat tcgtctgcgg gatgacgata
    40201 tcaaaacact ctttagccag gtcaccccag gcaccaaagt gaatatcatc aacactccga
    40261 taaaagtctc tgccgaacca aacggtgcgc gtctggttga agtacatcag ccgctgtcag
    40321 agaagattga tgacgatccg cagctgctgc caattacgct gaatagcgca atgcaatcat
    40381 ttaaagatgc agcacaaact gacgctgaag tgatgcaaca tgtgatggat gtccgttccg
    40441 ggatgccggt ggatgtccgc cgtcatcaag tgagcccaca aactctgtaa atcctggata
    40501 aaaaaaatcc ccgcgacacg gcatgtcgcg gggaaaaaat cagcagtagc aggcattaat
    40561 gagggttaat gcttatttat aaattactgc tgttccatgg agggtattcg gaccggttac
    40621 agaagtaata cggaaagatt ttgcgcccat ctcatccgct ttttgcgcca gctgctcttc
    40681 cagcgatccc agatttgtcc ccgcgttagc actgatagta ccgacttttt gttggccttc
    40741 tggcgttgac tgaacttcga cagccgcaaa gttggcaaat gacatggagc ttaaaatcgc
    40801 cgcagcgatg aggtttttac gtttttcata atagtggcct tatgcagatg aatgacgtcg
    40861 caagaaatta cttaacgatc gataactaaa tgatagatgt gatctggatc acatacaaga
    40921 tattttttat aacaatcatt aattaattta aaaatcttta aatttcagtc aaatagaaag
    40981 caattatttt tatgggatct ggactggtga acggaggtga ttatttttat aataaccttt
    41041 cattcacagt caccaggtac aacggcatca tggcaactga ctcaacacaa tgtgtaaaaa
    41101 aaagccgtgg ccgcccaaaa gtgttcgaca gggatgccgc gcttgataag gccatgaaat
    41161 tgttctggca acacggttat gaagcgactt ctcttgcgga cctcgtcgaa gcaaccggag
    41221 ccaaagcgcc cacgctatac gcggaattta ccaacaaaga ggggttattt cgtgccgttc
    41281 tcgaccgcta tatcgatcgt tttgccgcta agcatgaagc acagctgttt tgtgaagaga
    41341 aaagcgtgga gtctgcgctg gctgactatt ttgctgccat cgccaactgc tttaccagca
    41401 aagacacccc ggcaggctgc ttcatgatca acaactgcac caccctctcc ccagattcag
    41461 gagatatagc caatacgttg aaatcacgcc atgcgatgca agagcgcact ttgcagcagt
    41521 ttttatgtca acgacaagcg cgcggggaaa tcccgaccca ctgtgacgtg acacatctgg
    41581 cagaattcct taattgtatt attcagggga tgtcgatcag cgcacgcgaa ggtgcatcgc
    41641 tggaaaaact gatgcagatt gccggaacga ctttgcgttt atggcccgaa ctggtgaaat
    41701 aagtcgcaaa aaaggcccct cagcctgagc gaatgagggg ccaaattgca gagtacagcc
    41761 ttgttatact ttgttattca aaatcagttg cccgttacta tctagcggga tctgcgtacc
    41821 cggatcgcgg tccatgcgga ttttgccctg ctgatcgcca atcttatagg tcacatcata
    41881 accgagcatt ttttctgact tgtcatacac cgttttacaa cgctgttgcg tagtcgtgta
    41941 agtatcgctt tcctggagat agccctggat ctggttaccg gcatatccac cacccagcgc
    42001 cccacaacag tggcgacatc tttaccgcga ccaccaccaa actgatgccc tatcacgcca
    42061 ccagcaacag cgccgagcac cgacccggta atgcgatttt catcctgcac cggtcgacga
    42121 tgggtcactg tgacgttgcg acactcctga cgcggtgttt taaccgtttc cttgattggg
    42181 gttgcagaaa caacctgagc gtattgcggg ccccgttcaa acacgttcag actggccact
    42241 gccgctacgc ccaacgcagc tgcgacacca atcccgatac ccgccaacat tgatttattc
    42301 acgggaacac ctccttcttt gcagttatgc caatcatgca agcgaaggag aaaagcagct
    42361 atcagacaga agatgaaaag cgcgggaatg actggaaata cttaaggatt cggaggagtc
    42421 tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag gcctaccgct cgtaccattt
    42481 gtccagggcg gataaggcgt ttataccaca tccgccagtg tacgtcgatt aatgcaactt
    42541 caaacgcgga cggataacgc ggttaatact ccccaccagc atcattaatc cggttttaaa
    42601 gtaaccatgc agcgcaatct gatgcattcg gtatagcgag atatatacaa agcgcgcaat
    42661 tcgtccttca atcatcattg agccgcgcgt caggttaccc atcaggctac caacggtgga
    42721 gaagttcgac agcgatacca gcgaaccatg atctttatac tgataatttt tcagcggctt
    42781 accgttcatc tgcgccagaa tgttgttcat tgcgcaagtc gccatctggt gtgcagcctg
    42841 agcacgcggc ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt cgccaatagc
    42901 gtaaatgtct ggatcgcgag tggtttgcag cgtcggttcc accaccagct ggttgatacg
    42961 gttagtttca agaccaccga tatctttcag gaagtctggc gctttgatcc cggctgccca
    43021 taccatcaga tcagcctcaa tatattcgcc atctttagtg tgcaggccgc cttcatcagc
    43081 actggtgacc atggtttgcg tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc
    43141 cgcagcagag atacgtggcg gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt
    43201 tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag ctgtgcagtt gcttgatcgc
    43261 gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt
    43321 cactttgcca tttgcgccca ggttggcaga gtatttcagg aacagattca gcatctcctg
    43381 gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg cagttctctt tgacgcctgg
    43441 cgtattgaaa tcgttagagg tgctacccag cgccatgacc agggtgtcat aggcgatttt
    43501 acgttccgga accaacagtt cacctttctc gtcgcgtagt tctgcaatag tgattgtttt
    43561 cgcttcacga tcaatatcaa tgacggaacc caactggaac tggaaaccat gattgcgcgc
    43621 atgggccaga tagctcaacg catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg
    43681 cagcagtggt ttccacaggt ggctgtggtt acgatcgacc agcgtaattt tggctttttt
    43741 cttacgtccc agcttatgcc ccagctgtgt tgccatttcc agcccaccag caccgccgcc
    43801 gacaatcaca atctttttca atggcgtagt caacgtgatc cccttaaatt tattaaccaa
    43861 ttgttaatta atggttatta acatagctct taattaacaa caggttacag caatgaaaat
    43921 gttcttcgct attgagaata acatgaatgg tgcattggtc ataccaaaat tgatgttaat
    43981 caagttttgt tgcttaaaag ataggcaact ttaccggggg agatcaaaaa atagagttta
    44041 ttgtttatta tttgagcaaa ttcggaaggg taatataggg tgaagcgtat atcgagggcg
    44101 tttggtcgtc ctgataagat gcgcagcatc gcatcaggca ttgatgctcg actgtcggat
    44161 gcggcgtgga cgccttatcc gacttacaga attcggagcg catcaggtac cggcaaggtt
    44221 acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg ctgtaaatgc
    44281 ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc gtaataatga
    44341 tgcaactctt cagatgtccg ctggctgtta agtgcttcat cattacgcga caaaataacc
    44401 agacaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc aatatcggca
    44461 tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa attagggttg
    44521 aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta tccgcccagg
    44581 ccaacgccgc aaattagcgg acgctcgtca acgttcagtt gcaacatttt gtccacttct
    44641 ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat caagcgtaca
    44701 tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact gttagagtca
    44761 aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt gacctgcttt
    44821 ctcttcctgc cagcgttcat gtaactgatt cagacgggtg ctgatcgctt tccagcgagc
    44881 cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg taacacgttc
    44941 tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat tattgcagac
    45001 caggatcata tcgcaacccg catccagtga tgcctgcccg cgttcggcat aactgcccat
    45061 aatcgcggca ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa aacccagttc
    45121 ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg gatcaacatc
    45181 actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga ttaaggaact
    45241 gaagaccgac atatctttag cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc
    45301 tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat
    45361 tccggcttca tgcataccat caataaaccg gctggcaatt gccagggctt tttgtggatc
    45421 ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacatcca gcactggcgc
    45481 aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac ccgcctcttg
    45541 cgccagtttg ccgccctctt ccattcccaa cagcgcagca aaggattgtg ctgccggtaa
    45601 gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct tcctgatcta ccgccaccac
    45661 cagatgattg cgcgatgctg cgcggatctg gcgcaccagt tcacgtaact gggcaggatc
    45721 atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcca gtatttcacg
    45781 ctcttccgcg tccagttcgt aaccttcgac atccaacatt actggaccca cactgctctc
    45841 cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata aattgttgat
    45901 cgccggtttg tcgccagcgg tactcaaacc accctccttt gagcatcagc agccagggaa
    45961 accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga gtggcatagt
    46021 cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc agttccagcg
    46081 cgatatcacc atctccagca tactcccagt cgatgagttt taaccctgat gcgctatgca
    46141 ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggt cgtggttccc
    46201 gcgctttgcg cagacgtttt aacattcgca gccaacccac agtccgccgc gccggatcgc
    46261 tttgctgcca gtacagttcc agtaagggca acagcgttat tcgccagcca aaacgtggtt
    46321 gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa tacgttttta
    46381 ccgcgccggg cagatagtcg actaccatcc agtcacggag atataaatgc ggcttcggtg
    46441 caatgcttgc gggtagttgt gataaagccc gatactggcg caagaacgcg gactgcggcg
    46501 catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctgatgttca atgagaaaac
    46561 taccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg
    46621 acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa cggcaccttt acctgaccag
    46681 ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc gttaacgttg
    46741 ccagaggcgc tggagtacag cacgtaatga gcgccgacat tgcgggcaat gcctatggct
    46801 ttactacggg tgcctaaact gtcctgcggc gacaaaccta actgttgctt agccatcgac
    46861 agttgctggg cggaaaccag ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt
    46921 tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct atcaaccagc
    46981 aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat cggctgcatt
    47041 gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg gtgccgttcg atcttcgtgc
    47101 tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt
    47161 tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc aggttcacgt
    47221 tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt
    47281 tttgtcatca agattcaccc ctttacaaat atagataaag tctgactttg tgcgccccca
    47341 gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc gggaatggta acgctgcgtg
    47401 gcctttccag aggatgcatc tccagccctc tggcgtcata ccagtaaaaa cgataatgaa
    47461 cggtaacggg ttcttgcctt tcgttataaa gcgttgagga tgctgaaggt tgaatatcag
    47521 acgtcgaaag gacgggcttt tctgcactga tgcccgcagc caataaagat gactccatca
    47581 ccagcgattg ctcatcattc accggaattt ccggatgtga acgacagccc accagcagta
    47641 acaacaccag agacaccagc ccaaagcatc cttttctcat cgttacagac ctttatgcgc
    47701 cagcattggt cccagcggac ggccacccaa caagtgcata tggatgtggt aaacctcttg
    47761 tccgccatgg cggttggtat tcatgatcag acgatagcca tcttcggcaa taccttcttg
    47821 ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc gcctgctcat gctcagctga
    47881 cacgtcgttc acagtcggaa tgagaatatt cggaatgatc agaatatgcg ttggcgcttg
    47941 cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg tagacgatat cggaggggat
    48001 ctcacgacga ataattttgc tgaatatagt ttcttctgcc acgacatttt cctttttcaa
    48061 atagccatgc gttgcgccat gctacgccgc gccacactgc gagtatagag tatggtcgag
    48121 ttaaacctgc tctttcaatc ttaactcacg atttcttaag caaaaaatcg ttttgctgcc
    48181 gtatatatcg ccattattcc catttctgcc tgcgaatgcg ctgaaaagac attctctgtt
    48241 tggtttacac aacaaatatg aatgcgtata tttctcattt gcatttgcat ttgcagcaaa
    48301 attattcgca cactaaatat aaccgctcat caccctcctc cggcatgagc ctgacgacaa
    48361 cataaaccaa gagatttcaa atgctttcaa cacaatttaa cagggataat caatatcaag
    48421 tcatcaccaa accgtcacta cttgccggtt gcatagcact ggcactatta ccttctgccg
    48481 cttttgctgc accagccact gaagaaacgg tgattgttga gggttcagcc acagctccag
    48541 atgatggcga aaatgattac agcgtaacgt ctacctctgc gggtaccaaa atgcagatga
    48601 ctcaacgtga tattcctcag tcggtcacta ttgttagcca gcagcggatg gaagatcagc
    48661 agttacaaac gctgggcgaa gtgatggaaa acacgctggg gatcagcaaa agtcaggcgg
    48721 attccgatcg tgctctttat tattcccgcg gattccagat cgataactat atggttgatg
    48781 gtatccccac ctattttgaa tcgcgctgga atctgggcga cgcactttct gatatggcac
    48841 tgtttgaacg cgtagaagta gtgcgtggcg cgacaggact catgaccggg acgggtaatc
    48901 catctgcggc aattaatatg gttcgaaaac acgcgacctg tcgtgaattt aaaggcgatg
    48961 tctcggcgga atacggtagc tggaacaaag aacggtatgt ggcggattta caaagcccac
    49021 tcaccgaaga cggtaaaatc cgcgcgcgaa ttgtcggcgg ctaccagaat aacgactcat
    49081 ggctggaccg ctacaatagt gaaaagacct tcttctcggg cattgtcgat gctgatttag
    49141 gcgatcttac gacgctttca gccggttacg aatatcagcg cattgatgtt aatagcccaa
    49201 cctggggcgg tttaccgcgc tggaatactg atggcagcag caacagttac gatcgcgcac
    49261 gcagtaccgc acctgactgg gcgtacaacg ataaagagat caacaaggtc tttatgaccc
    49321 tgaagcagcg gtttgctgat acctggcaag cgacactgaa tgccacccac tctgaagtcg
    49381 aatttgacag caaaatgatg tatgtcgatg cctatgtaaa caaagcggat ggtatgctgg
    49441 ttgggccata cagtaattat ggacctggct ttgatcatgt cggcggcacc ggttggaaca
    49501 gtggcaaacg taaagttgat gcgctggatt tgttcgctga cggtagttat gaattgtttg
    49561 gtcgtcagca caatctaatg tttggtggca gttacagcaa acaaaacaat cgttcagttc
    49621 atgggccaac atcttcccgg atgaaattgg cagtttctac aactttaatg gcaatttccc
    49681 acaagccgac tggtcatcac agagcctggc gcaggacgat accacacata tgaaatcgtt
    49741 atatgctgcc actcgtgtca cccttgccga tccgctgcat ctgatcctcg gcgcacgtta
    49801 taccaactgg cgggttgata cgctgactta cagcatggag aaaaaccaca ccacgcctta
    49861 cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc tacgccagct atacctctat
    49921 tttccagccg caaaatgatc gtgacagttc aggcaaatat ctggctccaa tcaccggtaa
    49981 caactacgag ctgggtctga aatcggactg gatgaatagc cgtctgacca ccacgttagc
    50041 catcttccgt attgagcagg ataatgtcgc tcagtccacc ggtacaccta tccccggcag
    50101 caacggcgaa accgcctata aagcggtgga tgggacagtc agtaaagggg tggaatttga
    50161 actcaacggc gcaattaccg acaactggca gctgacattt ggcgcaacgc gctatcttgc
    50221 agaggataac gaaggaaacg ccgttaatcc taatctgcca cgcaccacgg ttaaaatgtt
    50281 caccagctat cggttgcctg tcatgccaga gttgacagtc ggcggtggtg ttaactggca
    50341 aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc ttccgcgccg agcaaggtag
    50401 ctacgcgctg gtggatctct tcacccgcta ccaggtgacg aaaaacttct cgttacaggg
    50461 gaacgtcaat aacctgttcg acaaaaccta cgataccaac atggaaggtt ctatcgtcta
    50521 cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa ttctgaataa cacctgtaaa
    50581 aaaggcagcc atctggctgc cttagtctcc ccaacgtctt acggattagt ggttacggat
    50641 gtactcatcc atctcggttt tcaggttatc ggatttagta ccgaaaatcg cctgaacacc
    50701 agaaccagca accactacgc ccgctgcgcc cagtttcttc aggccagcct gatccacttt
    50761 agacacatca gcaacgctga cgcgcagacg ggtaatacat gcgtcgaggt tagtaatgtt
    50821 ttctttacca ccaaatgcag caaccagagc cggtgccatt tcgctggtac ctgttgcttt
    50881 tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga tccagtgctt taatcagcac
    50941 gcggaagatg gtgtagtaaa caatcgcata accgataccg acgatcggta acagccacag
    51001 tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga ccgtgcgaga acgacgtacc
    51061 gtcacgcatc cccagaagaa tacagattgg gaatgccagg cctgccagaa tcgcgtggat
    51121 gatgtacagg atcggcgcaa cgaacatgaa gaagaactcg atcggctcgg tgataccggt
    51181 caggaacgag gtcagcgccg cggagatcat aataccgccc actttcgcac ggttttctgg
    51241 tttagcagag tgccagatag caattgcggc agctggcaga ccgtacattt tgaacaggaa
    51301 gccaccagac agtttacccg cagtcgggtc acccgccata taacgcggaa tgtcgccgtg
    51361 gaaaacctga cctgctgcgt tggtgtattc accaatctgc atctggaaag gtacgttcca
    51421 gatgtggtgc agaccaaacg gtaccaggca acgttcgatg aaaccgtaaa tgccaaacgc
    51481 aactaccggg ttctggtaag cagcccactg agagaaggtc tggattgcag aaccaatcgg
    51541 cggccaaatg aaggacagca caacgccagt aaagatggca gccaggccag aaatgatcgg
    51601 cacaaagcgt ttaccggcaa agaagccaag atactcaggc agcttaatac ggtagaaacg
    51661 gttaaacatg tacgctgcaa tcgcaccgga gataatccct ccgagtacgc cagtatccgc
    51721 caagtgttta gaggcgattt cttcagcagg taaatgcagt accagtggcg caaccacggc
    51781 catggtttta accatgatgc cataggcaac aactgcggcc agcgcggata cgccatcgtt
    51841 attggtaaag ccgagggcga caccgatcgc aaaaatcagt ggcatgtttg caaagacgga
    51901 accgcctgct tctgccataa catgcgatac aacggcgggc agccagctga aattcgcgga
    51961 accgacgccc agcagaatac ctgcgatagg tagtacggat accggcagca tcagcgattt
    52021 accgaccttt tgcaggttag caaatgcatt cttaaacata attgagagtg ctcctgagta
    52081 tgggtgcttt ttttacgttc tcacgcgtgg caagggggga gagcctcgcc gtgtacaggg
    52141 catctaagcg ccctttattt attacacaga gtaaaataat tcagtgccaa tttgtttgac
    52201 ggctatcacg tttcaacttc agatcattgg agaagttgca cattttgaca aaacatatgt
    52261 cacaatattt caaacgcggt tattcaccgc ccaattttcg gcggtgaact ttactcgttt
    52321 tagccattaa ttacgagttt taaaaaaact cattcaacgg atggattgaa ggcgggaagc
    52381 gtcgatgtga aacagacggg cgaagttatc ggtggttacc tgcgccagtt cttcaacggc
    52441 aacacctttc aacacagcca tgtattctgc aacgtcacga accatcgcag gttgattctc
    52501 ttttcctcga tgcggtaccg gcgcaaggta aggtgagtca gtttccacca gtaatcgatc
    52561 cagggggaca taacgcgcag catcgcgcag ttgctccgca ttacggaagg tcacaatgcc
    52621 ggaaaaggag atgtaaaatc cgagatccag taatttaccc gccgtttctc tgtcctctgt
    52681 aaaacagtgt agtacgccac cgcaatccgt cactttttct tcgcgcagaa ttgccagcgt
    52741 atcggcacgg gcgtcacggg tatggacgat aaccggcttg ttcagttcac gaccaatctg
    52801 gatatgatgg atgaaggact cttgctgacg tactttagtt tccggtgtgt aataataatc
    52861 cagcccggtt tcacccagcg ctacaacacc ctcttctgcc gccagacggc gtaaatcttc
    52921 tacatcgtag ggatcattct ggttgagtgg atgcacgcca caagaaaata cgacgttgtc
    52981 acgttcgcct accagatccc gcatatgtaa ataacccggt aatgttgtgg cgactgccag
    53041 acaaaatttc acatcgcgtg cggcggcttt cgccagaacg tcatccacgt ccttatgcaa
    53101 agattcataa tccagaccat cgagatggca gtgtgagtcg actaaaaaca tgatgtctct
    53161 cttaaagatg aggaaccggt agcacaacgc ccggttgcag gtaatgctca atacgcagta
    53221 aaagatcggt gatgagaagc tcgcggttga tgcctgtgac agacattaac tgttcacgaa
    53281 tgtggcaaac atcccccagt atagcctgca ggcgcgaggg agaaagatgg tttgccagtt
    53341 cggcgaccag gcccggcaca tcaacattgg tcacctgcgc agcgccatga tggcgtttta
    53401 gcgcatccat cagcaacgtt gccagccagt gtaaacgcgc cggagcttgt tcatgattaa
    53461 gggccgccag cagcgaatac cagtcgcccg atggcacgct atatgccaac gcctgacaca
    53521 atgtttcacg agcctgccag ttatctccct gaaacaacgc cagtgccgcg ccaggcgaac
    53581 cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga cattgtcact tcgcgtgaaa
    53641 gccaagtcac ggcgtactgt tctggaggcg gcgcaaggta atgtaaccga caacgactac
    53701 gtaatgttgc cagtaaacgt tcaggctcgc gggtagccag gaaaaaccag gtttctgctg
    53761 gtggctcttc aagcgttttc agcaatgcgt tagccgcggc gtcggtcagt aaggcagcat
    53821 cggttaccca gacaactttc gcaccaccta ggcgtgcgtg ctcattcagc ttttcggtga
    53881 cctcacgtac cgcatcaatg cccagcgtat ttttcccttt ttcgggagcc agggtgtagt
    53941 aatcgggatg cgtgccagcc tgcatcaact gacatccacg acagtgaccg caacttttgt
    54001 ggccctgcgg ttgttggcag agtaaataac ggctcagggc gtagattaaa gcatcatcgc
    54061 ccatgcccgg taacgcctga atgagtagcg catggtgacc tcttccggcc tgatagctgg
    54121 ctaccagttt ttcgaaatca ggtcgtaacc atggatacca tctcatgcgt ccaactcctt
    54181 cacccagtgg gtcacggtag tgcggattgc atccatcacg gcctccagcg gctgggtggc
    54241 atcaatggta tgaatacttt tatcttgtgc agccagttcc agatagcggg cgcgggtgcg
    54301 attaaagaaa tcgaaagact cttgctcaat acgatccagc tcgccgcgcg cacgcgcgcg
    54361 ttttaagcca acttccgggg taacatcgag atagagcgtt aagtctgggc gaaaatcccc
    54421 gagaacagca tcacgcagtg ttgccagcat atgttggtca ataccacgtc ctccgccctg
    54481 atacgcctga gtggaaagat cgtggcgatc gccaatcacc caggtgccgt tagccagcgc
    54541 aggtttgatg accgtttcta ccagttgaac gcgcgcggca taaaacatca gaacttcggc
    54601 tttatcggta atgacttcat cgcctaccga tttgatatcc agcaccaggc ttcttaactt
    54661 ttcggcaagt tgcgtaccgc caggttcccg agtgaaaacc atgtcgcgga tacccagttg
    54721 ctcgagcgtc tcaaccacca cattacgcgc ggtagttttg cctgcgcctt ccagcccctc
    54781 aatgacgata tacttactgc gcattttttt ccttaagcac tttcagataa tcctgcacag
    54841 acttgttatg actggcaaga ttggtattaa acgtgtgacc acctttacca tcggccacaa
    54901 aatagagata cggcgttttt gccggatgcg cagcagcctt cagcgaatcc gcccccggcg
    54961 tagctatcgc acccggcggc aaaccggtaa tggtataggt gttatacgct gtcggcgttt
    55021 ccaggtctgc acgagaaagt ttgccattat aacgctctcc catcccgtaa atcacggttg
    55081 ggtcggtctg caggcgcata ccaatgcgta aacggttgat aaataccgag gcaacctgat
    55141 cgcgttcact ggcaacggcg gtttcttttt cgataattga tgccatcgtc accagctgat
    55201 ttttatcttt ataaggcaga ccgtccgcac gcccttccca ggcgctatcg accgctttca
    55261 ccattttctt gtgaactcgc ttgagtaacg cgacatcggt ggtattggcg gtatacatca
    55321 aggtgtctgg ccagaaccaa ccttcaatcc actccggatt ttccagttca agtgcctgcg
    55381 ctacggtggc gtacttatcg tcactcagcg tgtgcttgat atacggagcc tcacgcaatt
    55441 gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg cagcgggaac tgtgcttctt
    55501 taccgctttc caacaatttc agcatctcgc gcacggtcat ctgcggtgta aagcggtaag
    55561 tcccggcttt aaagtgagaa agatccggtt cgatacgcag cagccattga aaaacccgtg
    55621 ggcgattgat gatcttatca gcataaagct gttcaccgag cgccagacgt ccggtccctg
    55681 gcttcagggt aaatatcgtc tcttctttga taagcaattt gctgtcggca agatggcgaa
    55741 ccttccagac gcccacacca gcggcgatac ccagtaccac cagcaataac aagataatca
    55801 ataacacttt tttcatgact aattcgggcg ctcacaaagt ggggctaaat attcatataa
    55861 cgttgctgac gaaaaggtga catcgccaca ggcacgtacg ggcatcactg gcattaacgc
    55921 attacaaata accatctcat ctgcctgcaa cgcctcctcc agcgaggctt gcacttcgac
    55981 aagctgataa gaggattgtg ccagtaaacg gatacagaat tgtcgcataa tgccgttaac
    56041 acctgcctga tccagtcgcg gcgtataaac tacattgccc ttccgccaga acaaattagc
    56101 cgcacagcat tccgtaaccc acccttcgct gtcaaggacc agcgcctcat cagcgtttgt
    56161 ctgctcaaga tgagagcgaa tcaatacttg ctcaagccga ttaagatgtt taatacctgc
    56221 aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc aacgtcatcc cttcgttacg
    56281 caaacggtcg taatgtgcag gataagccgt aacggagaga atccgcgttg ctggtccgct
    56341 gttcaatgtg ctgtaccctc gcccgccact accgcgactg atcacgactt tcagcacacc
    56401 attttgctgt tctgctgcca gcgttttcat ctcttgttca agctgaggcc agaagtcaca
    56461 ggaaatcatc aaccgctgac aagcatcctg tagtcgcagg atatgcgccg ataacaaact
    56521 gactttaccg tcgataactc tggcggtggt aaaacaacca tcaccaaact gcgttgcccg
    56581 atcgcttact gccagcgatt cctgcttata accgttaatt aagaacatac cggctcctta
    56641 tcagagaatt gttagtgtgg cagcatgttc actacggaac aagtcggaat aaaagctaag
    56701 aaaaaaggcc cgcaagcgga ccttttatat gggtggaaaa tgacaactta gatcttttta
    56761 aagatcaaag aaccattagt gccaccgaag ccgaaggagt tacacagcgt gtattccatt
    56821 ccgctgacct gacgtgcttc atgcggtacg aaatccagat cgcaaccttc atccgggtta
    56881 tccaggttga tggttggcgg aacagcctga tcgcgcagcg ccaggatgga gtagatagat
    56941 tctactgcac ccgccgcacc taacaggtga ccggtcatag atttcgtgga gcttaccaac
    57001 acacggcttg cagcttcacc gaagatggtt ttcaccgcct gcgcttcagc tttatcgcca
    57061 gccggcgtag aagtaccgtg cgcgttaacg tagccaatct gactcgcttc aatgcctgca
    57121 tcacgcagag catttgccat tgccagagct gcgcctgcgc cattttctgg cggtgacgtc
    57181 atatgataag catcgctgct cataccaaag ccgacgagtt cagcgtaaat tttcgcaccg
    57241 cgttttttcg cgtgttcgta ctcttcaagt accagcatac cggcaccatc gcccagtacg
    57301 aagccatcac gctctttatc ccacgggcgg ctcgccgctt gcgggttatc attgcgggta
    57361 gataatgcac gtgccgcgcc aaaaccacca acgcccagcg gcgtactggc tttctctgcg
    57421 ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa tacgcgcagc atggccaatg
    57481 ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc ttgggccacg caggccatac
    57541 atgatagtca gatgacctgc caccatgttc acaatcgttg acggaacgaa gaatgggctg
    57601 atcttacgtg gaccaccgtt catcagagat gtgtggtttt cttcgatcag tccgaggccg
    57661 ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt tctcttccgt tatttcaagg
    57721 ccagaatcct gcatggcctg aacgccagcg acaattccat attgaatgaa ggcatccatc
    57781 ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa aatcctttac taagccagca
    57841 aatttcgttg cataggcgct agtatcgaaa tggtcgatta ggctgatgcc actctgaccg
    57901 gcaagcagag ctttccaggt agactctacg gtattgccga caggagacaa catgcccagt
    57961 ccggtcacaa ctacacgacg cttagacacg tttgtcctcc aaggagggaa aaatgattct
    58021 agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg cctggagatg ttcacttacg
    58081 cctggtggcc gttgatgtaa tcaatggcag cctgaacggt ggtgattttc tcagcttctt
    58141 cgtccggaat ctcagtatca aactcttctt ccagagccat taccagctca acggtgtcaa
    58201 gagaatccgc gcccaggtct tcaacgaaag aagcattgtt ggtaacttct tcctgcttaa
    58261 cgcccagctg ttcgccgata attttcttaa cgcgttcttc gatagtgctc atactcttaa
    58321 atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt gtataaaatg ttgaaaaatt
    58381 tgcaactaaa tcccggcagg tcttaccacg attttacgtt attttgaggc cttacccccc
    58441 aataacgcaa atcattttgc actaattatt actcataacc acgcagactg cgcgcaacat
    58501 gagcattttg tgcaaatcgc ggtcagacca tgtacatccc gccgttcaca tgcaaagttt
    58561 cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc aaccgcgttg gcgatttcct
    58621 gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat acccgcacgc tggtcatcgc
    58681 tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc aacaacgttt acagtaatac
    58741 cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc gatcaagccc gctttcgccg
    58801 cagcgtagtt ggcctgaccg ccatttccca tggtaccaac cacagaaccg atagtgataa
    58861 tacgaccatg acgctttttc atcatagcgc gcattaccgc ttttgacaga cggaaaacag
    58921 atgaaaggtt agtttcgata atatcgttcc actcttcatc tttcattcgc attaacaggt
    58981 tatcacgagt gataccggca ttattgacca ggatatccac ttcaccaaat tctgcgcgaa
    59041 ttttttccag aacagattcg atagatgccg ggtcggtcac attcaacatc agacctttgc
    59101 cgttggcacc taaataatca ctgatcgcct gagcgccatt ttcactggtc gcagtgccaa
    59161 taactttcgc gccacgggct gcgagcgttt cagcaattgc gcggccaatt ccgcggcttg
    59221 caccggttac cagtgcgatt tttccttcaa aattcatgat tttcctcttt taaagctcga
    59281 gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc ggtcagggtg tcgacaatgc
    59341 gtttcgtcag gccagtaagc accttgcccg ggccgacttc atagagatgt tctacgcctt
    59401 gcgctgccat gtactcaaca gacttcgtcc actgaaccgg gttatacaac tgacgtacca
    59461 gtgcgtcacg gatggcatca ccattggttt cgcatttcac atcaacgtta ttcacaacag
    59521 aaactgttgg tgcgttaaag gtgattttcg ctaattctac tgccagtttg tcggctgctg
    59581 gtttcatcag cgcacagtga gacggtacgc tcactggtaa cggcagcgcg cgttttgcgc
    59641 cagcagcttt acaggcagcg ccagcacgct caaccgcttc tttatgaccg gcaataacca
    59701 cctgtcccgg agagttaaag tttaccggag aaacgacctg accttctgca gcttcttcac
    59761 acgctttcgc aataggcgca tcatccagac cgatgattgc cgccatagcg cccgtgcctt
    59821 ccggtacggc ttcttgcatg aacttaccgc gcatctcaac cagacgcacc gcatcagcga
    59881 aatcaatcac gccagcgcaa accagcgcgg agtattcccc caggctgtga ccggccatca
    59941 ttgccggtgc tttaccgccc tgctgctgcc atacgcgata cagcgcaaca gatgcagtca
    60001 acagcgctgg ctgggtttgc caggttttat tcagttcttc agctggcccc tgctgggtca
    60061 gcgcccacag gtcgtagccc agcgccgcag aagcttcagc aaacgtttct tcgacaattg
    60121 gatagctcgc cgccatatca gccagcattc caacggtttg agaaccctgt ccagggaaca
    60181 caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa acgaaccagc gcagagcccc
    60241 aagtgaatcc accgccaaag gcttcaagca gaaccaactg ccccggctta atgcgcccgt
    60301 cgcgtacagc ttcatccagc gcgcacggga cagaggccgc agaggtatta ccgtggcgat
    60361 ccagcgtcac cacgacattg tccatcgaca taccgagttt tttcgccgtt gcactgataa
    60421 tacgcaggtt agcctgatgc ggaaccagcc agtccagttg agaacggtca agattattcg
    60481 ccgccagcgt ctcatcaacg atgtgcgcca gttccgttac cgcaaccttg aagacttcgt
    60541 tgcccgccat cgtcagatga attgaattct ctggattcac gcggtcggcg tttggcagcg
    60601 tcagcaattc accataacta ccgtcggcat gcagatgggt ggaaatgatt cccggctctt
    60661 cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa aataataata gtcccacgat
    60721 ctgttggatc gcaggtgcgc gccagtacat cggaaccgac gaccagagca tacttcaccg
    60781 ccccagattt cacgtattga tcggctacgc ttaatgcata ggtgaaacct gcacaggctg
    60841 ctgcaacgtc aaatgccggg caacctttaa tgtccaacat gctttgaatc tgacaagctg
    60901 cgctcgggaa agcgtgcgta gcagaagtcg ttgccacaac gatcaggcca atctggtctt
    60961 tctcaatgcc cgccatctca attgcgcgtg tcgccgcttc aaagcccatg gttgaaacgg
    61021 tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt acgagtgaca atccactcgt
    61081 cagaggtgtc caccattttt tccaaatcgg cgtttgtccg cacttgttcg ggcagatagc
    61141 tgccagtacc aataatcttc gtatacatgt acgctcagtc acttttcggt tatataccgt
    61201 cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc gcagagttcc gcttttgcca
    61261 ccgtccagca gctcaaaacc agctgggtat acagattcca ggcgagcggc aattcgctga
    61321 ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg cgaccgcaaa agctcgctga
    61381 ttggctgcac catgactttt tatcaccgtg ccgcgcaatc ctaacagaca ggcgccgtta
    61441 tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc tcttttgtag ccaacgcttt
    61501 aataacagta gccaccacga ccgttttttc ccttcaccct gagatttcag cagagaaagg
    61561 aacatcctga caacaccttc catcgtcttt aacgtgacat ttcctgtaaa gccgtcacaa
    61621 accagcacat ctgtcttgcc agttaacaac tcattggctt caagatagcc gatataattg
    61681 atagaaggga ttgttttaag caccgctgag gcatcccgaa tactgtcaag accctttact
    61741 tcttcttcac caatattgag caacgccacg cgaggattgg gaatttccac cacctcttca
    61801 gccagaactg agcccataat ggcaaattgc accaacattg tgctgtcaca atcgacgttg
    61861 gcccctaaat cgaggaccac cgttttgccc ttttgctgat gtggtaatac cgtcaccagc
    61921 gccggacgct caatcccctc caggggcttg agtaataatt ttgccagccc catcagtgcc
    61981 ccggtattac cggcactgac acaggcttgc gctcgacctt ctttcaccag ctccagggcc
    62041 atacgcattg aactcccacg actggcgcgg atagcttgcg aaggccgggc atcactggcg
    62101 ataactgact gcgcaggaat aatctgcaga cgcgaacgtt gttcaaagtc agctttagca
    62161 agtaatggcg tgatggcgtc gggattgccg actaaaagaa gagtgagttg cgaattagaa
    62221 ttcagtgcct gcaatgctgc aggcactgtc acggaagggc caaaatcccc tcccatgaca
    62281 tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc ccggtttggt ctttccccag
    62341 ttgcctgggg aaaatcctca ctaagcttca tcacgcagac gcgtgattac ttagcgatga
    62401 ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg acgcaggtgt ttttcaccag
    62461 aagttttgtc tacagacagg ctggtgactg cggtcagcgc gtcatgggaa cgacgcatgc
    62521 cacgtttgga acgggttggt ttattctgtt gtacggccat ggaccttact cctcaattac
    62581 ggtttatccc cgatccaacg gggagcacca attacttacg ctttaagctg gctaatacgg
    62641 caaatgggtt tggcttttgc acttcttcag gcagttcacc aaagaccatg tccgcttcgg
    62701 acacttcaca gtgttcagaa tcatgcaccg gaactaccgg caaggcgagg atgatttcat
    62761 cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt aacctcaatc ggttcatacg
    62821 cttccggcag tgcttcagcc tgttcgtctg aacgcacagg actaaaacaa tacgttgtgt
    62881 agacctgatg agtaaacggc ttcccgcaac gctgacactc gagcgttacc gtcaccttcg
    62941 catcgccgtt taacactgcg agacgttggt tatcgatagc gaacgacatg gagcattcca
    63001 catcactgtc cacactgact acggattcgg cgacgcgctc aacctgatca ggggtataga
    63061 taccctggta atcaaggcgt ttttgagccg tacgaaccgg atcgagagtc aggggtaatt
    63121 ttaccttttg catagggcgc gcatattaac tttgtaacgt catagagtca aagaaaaagg
    63181 cagcctgtgg ttgccttttg ccaataattc gcacacattg cgggttactg ctttattttc
    63241 gttcagtgct gataacgaac atcaacctgc aaaacgaagc gcatagttta aaatggctat
    63301 catcaatacg ctatatctga tggaaaaaaa tatgcctaaa cttattttag cctccacctc
    63361 gccatggcgt cgcgctcttc tggaaaaact gcaaatctct tttgaatgtg cagcacctga
    63421 ggtcgacgaa accccacgca gcgacgaatc accgcgacag ttggtgcttc gactggcaca
    63481 agaaaaagcg caatctctgg cgtcacgtta tccggatcat ttaattattg gttcagacca
    63541 ggtatgtgtt cttgatggcg aaatcaccgg taaaccgtta acggaagaaa atgcccgtct
    63601 gcaattacgc aaagccagcg gcaatatcgt caccttctat actggactgg cgttgtttaa
    63661 ttcggcgaat gggcatctac aaacagaagt ggagcctttt gacgtccatt tccgtcacct
    63721 aagcgaggcg gagattgata attacgtgcg taaggagcat cccctgcact gcgcgggtag
    63781 ctttaagagt gaaggatttg gcattacgct gtttgagcgt ttagagggac gtgaccctaa
    63841 cacgctggtt ggtttaccgc taatcgcgct gtgtcagatg ttacgtcggg aagggaaaaa
    63901 cccgctgatg ggatagttgt ttgcgccgga tgcggttacg tcgcatccgg caatcggtgc
    63961 agacggcctg atgcggcgta aacgccttat caggccaacc gcccattatc aggcttatat
    64021 gcttgtatta gcgcgcgtta cgcagctttt gcaaacaacg cttcaaacct tcatccatcg
    64081 gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt aaacttcaac gctgcggcgt
    64141 gcaggaacag acgatttaat cccgtgcctg cttcagtgag ctgtctgtca aattcacggt
    64201 caccgtagcg atcgtcaaag gcaatcggat gacccgcata ctgtgtatgc acacggatct
    64261 gatgagtacg tccggttacc gggctacaac gcaccagggt ggcaaatgca tagcgttctt
    64321 ccactttaaa gcgtgtttcc gacggtttgc cttcctgact gacacgcacg atacgttcgc
    64381 cgctttgcag aatatttttc aataacggcg cttgaacgct cttcacatgc gactgccact
    64441 gaccgcgcac cagcgccagg taatcttttt gcattccttt ttcacgtaat tgctcatgca
    64501 gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac acctgaagtg tcccggtcga
    64561 gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa ccttcgataa
    64621 cgccgaagct taaaccactg ccgccatgta ccgccgtacc ggaaggttta ttcagcacca
    64681 ggatatgatc atcttcatat aagatgacgt ccgccagcgc cgccaccttt tgcagatgtg
    64741 gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg tgggatacgc acctcatcac
    64801 ccgcttcgag tttatattca ggcttaatac gttttttgtt cacccgcact tcacctttac
    64861 gcaaaatacg gtaaatcata ctttttggta cgcctttcaa ttgggtacgc aaaaagttat
    64921 cgatacgttg ccccgcttcg tcagcggtga tagcaacaat ttttacggat ggagtctctg
    64981 ttttcatggt gggcgattct aattagccaa caggattcgc gccactcatt tttctatgct
    65041 tatatttagt tttgcacctt attacttcac tacgtgatca ctttattgat ggttattaaa
    65101 ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg gtaaaagtca
    65161 tcttgctata acaaggcttg cagtggaata atgaggccgt ttccgtgtcc atccttgtta
    65221 aaacaagaaa ttttacggaa taacccattt tgcccgaccg atcatccacg cagcaatggc
    65281 gtaagacgta ttgatctttc aggcagttag cgggctgcgg gttgcagtcc ttaccggtag
    65341 atggaaatat ttctggagag taatacccag tctgtttctt tgataattgc gctgtttttc
    65401 cgcatgaaaa acgggcaacc gacactctgc gcctctttga gctgacgata accgtgaggt
    65461 tggcgacgcg actagacacg aggccatcgg ttcacacccg gaaaggcgtt actttgcccg
    65521 cagcttagtc gtcaatgtaa gaataatgag taagttacga tgaaaagaat gttaatcaac
    65581 gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg ggcagcgtct gtatgacctg
    65641 gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca tctacaaagg taaaatcacc
    65701 cgcattgaac cgagtctgga agctgctttt gttgattacg gcgctgaacg tcacggtttc
    65761 ctcccactaa aagaaattgc ccgcgaatat ttccctgcta actacagtgc tcatggtcgt
    65821 cccaacatta aagatgtgtt gcgtgaaggt caggaagtca ttgttcagat cgataaagaa
    65881 gagcgcggca acaaaggcgc ggcattaacc acctttatca gtctggcggg tagctatctg
    65941 gttctgatgc caaataaccc gcgcgcaggt ggcatttctc gccgtatcga aggcgatgac
    66001 cgtaccgaat taaaagaagc actggcaagc cttgaactgc cggaaggcat ggggcttatc
    66061 gtgcgcaccg ctggcgtagg caaatctgct gaggcgctgc aatgggattt aagcttccgc
    66121 ctgaaacact gggaagccat caaaaaagcc gctgaaagcc gcccggctcc gttcctgatt
    66181 catcaggaga gcaacgtaat cgttcgcgcc ttccgcgatt atttacgtca ggatatcggc
    66241 gaaatcctta tcgataaccc gaaagtcctc gaactggcac gtcagcatat cgctgcatta
    66301 ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg gcgagatccc gctgttcagc
    66361 cactaccaga tcgagtcgca gatcgagtcc gccttccagc gtgaagtccg tctgccatcc
    66421 ggtggttcca ttgttatcga cagcaccgaa gcgttaacag ccatcgacat caactccgca
    66481 cgcgcgaccc gcggcggcga tatcgaagaa actgcgttta acaccaacct cgaagccgcc
    66541 gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg gcctgattgt tatcgacttc
    66601 atcgacatga cgccagtacg ccaccagcgt gcggtagaaa accgtctgcg tgaagcggtg
    66661 cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc gctttggcct gctggaaatg
    66721 tcccgccagc gcctgagccc atcactgggt gaatccagcc atcacgtctg cccgcgctgc
    66781 tccggtaccg gtaccgtgcg tgacaacgaa tcgctgtcgc tctctattct gcgtttgatc
    66841 gaagaagaag cgctgaaaga gaacacccag gaagttcacg ccattgttcc tgtgccaatc
    66901 gcttcttatc tgctgaatga aaaacgttct gcggtgaatg cgattgaaac gcgtcaggac
    66961 ggtgttcgct gcgtgattgt gccaaacgat cagatggaaa ccccgcacta ccacgtgttg
    67021 cgcgtgcgca aaggggaaga aacgccgacc ttaagctaca tgctgccgaa gctgcatgaa
    67081 gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac gtaagcgtcc tgaacaacct
    67141 gcactggcaa cctttgccat gccggatgta ccgccagcgc caaccccagc tgaacctgcc
    67201 gcgactgtcg tagccccagc accgaaagct gcaacggcaa caccagcagc tcctgcacaa
    67261 cctgggctgt taagccgctt cttcggcgca ctgaaagcgc tgttcagcgg tggtgaagaa
    67321 gccaaaccga ccgagcaatc aacaccgaaa gcagaagcga aaccggaacg tcaacaggat
    67381 cgtcgcaaac ctcgtcagag caaccgccgt gaccgtaatg agcgccgcga cacccgtagt
    67441 gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc gtaatcgtcg ccaggcacag
    67501 cagcaaactg ccgagacgcg tgagagccgt cagcaggctg aggtaacgga aaaagcacgt
    67561 accaccgacg agcagcaagc gccgcgtcgt gaacgtagcc gccgccgtaa cgatgataaa
    67621 cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag agcaatctgt tcaggaaacc
    67681 gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta aacagcgtca gctcaatcag
    67741 aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag tcgcaccggt ggttgaagaa
    67801 actgccgctg ccgaaccaat tgttcaggaa gctccagctc cacgcacaga actggtgaaa
    67861 gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc aagaagagaa caatgctgat
    67921 aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct cgcctcgtca cctgcgcgtg
    67981 agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc caacccagtc gccaatgccg
    68041 ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca aagtctggat ccgctatcca
    68101 attgtacgtc cgcaagatgt acaagttgaa gagcagcgcg aacaggaaga agtacaagtg
    68161 cagccgatgg tgactgaggt ccctgtcgcc gccgctgtcg aacctgttgt tagcgcgcca
    68221 gttgttgaag aaatggctga agtcgtagaa gcccccgtac cggttgccga accgcaaccg
    68281 gaagtggttg aaacgacgca tcctgaagtg attgccgccg cggtaactga acagccgcag
    68341 gtgattaccg agtctgatgt tgccgtagcc caggaagtcg cagaacacgc agaaccggtg
    68401 gttgaaccgc aggaagagac ggcagatatt gaagaagttg ccgaaactgc tgaggttgtg
    68461 gttgctgaac ctgaagttgt tgctcaacct gccgcgccag tcgtcgctga agtcgcagca
    68521 gaagttgaaa cggtggccgc ggttgaacca gagatcaccg ttgagcataa ccacgctacc
    68581 gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg aggcaccgcg tcacagtgac
    68641 tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg caggtggtca tacggcaacg
    68701 catcatgcct ctgccgctcc tgcgcgtccg caacctgttg agtaataatt agctcaaagt
    68761 aatcaagccc tggtaactgc cagggctttt ttatttcatg tttgaatcaa catattaaaa
    68821 ttcatcaaat ggcatccttg ctaaccaaca atgcaaaata ggcaaaaaaa atgcccgccc
    68881 cggcgatccc agagcgggca gacaaaacat acccagtttc atgatatgtt ttaaagcgag
    68941 cttatttgtt gagctggaag agcgacaatc cctgcatatc ggtaaatgct ttatacgatg
    69001 cctgcaatgc cgtttgctgc atgatgtaag atgaaatagt tgcattccag tccacatcaa
    69061 ccagatcgct catctgctgc gtttgcccta aagcgcgatc gctacctaag gaatccagcg
    69121 actccagttt gttcagctgc gtgcctaatt ccgcgcgaac ggtaaggaca ttattcagcg
    69181 agtttttcag tccacggttg gttttatcca atgccgcagc ggcgatttct ttatccgctt
    69241 cgctatccgc gaccggcgtt ttcagggctg cgatggcgct atccagcatg gcaaaaagat
    69301 tggtttcaga agcgctaccg tctggttccg ctaccgcgtt actggtaata ctgtcgaaaa
    69361 ttttgtcgcc cgtatgtcct atcaccatcg aacgcgaagc atcgacctgt tgcttaatac
    69421 tttctgttcc gccaacataa tcaccgttag cctcactaaa cggcgcagtc tctgttttat
    69481 aaccggcaaa aatgtagcgc ccattaccgt cagtggtgtt cgccagattc agcaactggt
    69541 cacgaagccc ctgaatatcc gtagccagcg aggcccggtc atcgtcactc aaggtgccat
    69601 tgctggcgta gacaattttt tcctgagcat tctggatagc agtggtgacc tggctaagta
    69661 cactctcttc cagtgacact ttttgagtgg cgaaagtacg tgccagcgtg tactggctgt
    69721 tttgcgcctg tgcctgggag agaacaacag cttgtgatgc agcaatggga tcgtctgaag
    69781 ggttaacgac tcgcttaccc gtcgacatct gctcgccgta cttcatccat tctgcctgag
    69841 aattggtgat gccgcgcatg ttttgctggt acatcatctg ggtactgaaa cgcattttat
    69901 ctccccttag cgaatgttaa tcagcgcatc aaaaatcgcg tttgccgtct gcagaacctg
    69961 cgcattcgcc aggtaatact gctgaaaacg ttgcagattt ccgtactcct catcgagatt
    70021 gacaccggaa atcgactgct gctgattgga aagctgcgtc accacattac cttgcgtggt
    70081 gctactggtt ttcaacgtcg cggttttatt accgatatca ctcactaacg aggcataagc
    70141 gtcgttaaag gatttcgcac cgcccaccgt tttactgttg ctttgcagat ccagcagggc
    70201 ctgaccgttg cggttgtcgc tatcacccgc atcttcttcg ctcgccatcg cgattttcgc
    70261 ttcgtcggtg attaatacat ccatgttgac gatggcgtca cttactggtt tcagcgtgaa
    70321 gctgtcgtta acggcaggcg ttcctgtaaa cgtcaactcc agaccatcaa atgccacttt
    70381 accgttggca tctggtgtcg ccgtaaaagt ggtattgctg gcaaggcggg tgacctgcca
    70441 ctgattatta tcgaacgaga ttttgtaatc tgtcgccagt accacggagg catcagttac
    70501 cgtggcaccg atcgcaacgt cacctttgtt tttcgtgttt tgcagaaccg cgggcttacc
    70561 gatagtaaag aaatcttcac cagcatcgcc gttagcatca aatccggctt tgtgttggct
    70621 gttgaaagcc tcggcaaatg ccagcgccag ttgtccaagc gtattacgcg tctggtccag
    70681 atcctgagaa cggaatgtca gaatgccgcc cagcgacccg gtattcagta atttctccgg
    70741 gatctcaata ttgcctgccg tcccatcaac gtaagcgaca gtcgtacgag aagggtcggc
    70801 gctggaagga actgccgcca gttgccgcgc cgtacttccc tgaaccagtg agtaaccatt
    70861 ggcatcgtga tgttataagt accgccatcc tgaacgctga cttctacgcc aacaatctgg
    70921 tttaattcgc tcaccagttg atcgcgttga tccagcagat tgttaggtga cgcccctgcc
    70981 cccacgcctg tcaggcgcga gatttgatcg ttcaggctgg caatttgttt agcgtaattg
    71041 ttgatctgat caacgctggc acctatcgcg atattgacct gtttgtcctg gtcgcgcaga
    71101 tattgatcgg tggttttaaa ctgattcacc aacccttctg atttcccaat cagcgcctgg
    71161 cgcgccgccg ggtcttccgc gttactcacc agcgtttgca ggctggtgaa gaaatcctgc
    71221 atctgtgttg ccagcgaaga ggtactggtg gagagcatat tgtcgatttt cgacatctgc
    71281 tcatagcggg cggtcaggcc gctactttgc gtctgcgcag cacgtaactg gttggtaata
    71341 aacgtatcat actcacgctg cacaccagaa acgtagacgc cattgccaac ccagccgcca
    71401 gcgcccaacg tgctattggc ctgcgccata atagtggttt ggcgggtata tccggcaacg
    71461 ttatagctgg agatattatt acttgccgta tttaacgccg cctgggccgc gttcagtccg
    71521 ctcatggcgt tattgatcaa gctggacatg gaggttcctt ataagccttc aattacagag
    71581 tattatcggc agcgacccgc cggacttgag ttattcagaa cagattatca atgttcatgc
    71641 tgtaggtttt gctcaccttg tcgcttatcg atttcatctg ctggatcatg ttggtgagtt
    71701 tacgggcata gtgaggatcg gtagcatagc ccgcgtcctg taggacctgc gccccctgtt
    71761 ccgcactcgc ggcggtcgtc acggcggcgt agcgcgggtt acgtgttaac agcccaacgt
    71821 aatccgacaa tgcttccaga tacgagctgt agacccgaaa cttcgctttt actttcttcg
    71881 cttcgccgtt ttcatattca gtcgtggtga tttcagtgac ttgccctttc cagttgccag
    71941 aggctttgac accaaacagg ttatagcttg gctcgccgtt ttcgcggcgg atttgccgtt
    72001 gcccccaacc agattcaagc gcagcctgag cgaggatcaa atgatgtggc acaccgcttt
    72061 gctggcttgc cagttgggcg ggcagcgaga gttgcgcgag gaatgctcta ctgtcacccg
    72121 gcagcgaatc atcgtagtta cgtggtacgg ccttttgcac cagctgcgaa agcgtctggt
    72181 tttgataacg caccacggtt tcgagcggga atttcatcgg tgctgctggc gtggactcct
    72241 ctggcaatgg ttgttctggc gtcatctgtt taaccatcat ctctgcaagc cccagacctt
    72301 tgcccgccgt catctgttgg gcaatttgct ggtcatacat actggtatac agacgagtgt
    72361 gctcgctgct gaacaggcca tcttttggta aagcgtcgcg catgcttttc aacatcatct
    72421 gcacgaacat cccttccacc tgacgggcca ccggacggat atttgccgcc ggatcttcgc
    72481 tcgctttcgc ctttagttcg ttgagtgatt gcgcatccca ggccgcactt gccagtagtt
    72541 tgctgtcgct gatcatcaga tgatttccag ttttgcccgc agacatcccg cactttgcat
    72601 tgattgcaaa atagacatca gatccatcgg cgtagcgccc agcgcattga gcgcacgcac
    72661 cacgttattg aggctggcgc tggaacgtac gctttgcagc gaaccgccgc tctggcgtaa
    72721 atcgatctgc gtttgtggag taacaacagt ctgtccacca ccaaacggtg tatctggctg
    72781 gctgacattg gcctgacgat taactgttac tgagagattt ccctgcgcta ccgcgcagct
    72841 gtcgagggtc acttcgcgat tcatcaccac cgaaccggtg cgcgagttaa tcactacttt
    72901 agcgtcctgc ggggtgacat taacctgcat attctggata tcggcaagga aacggacctg
    72961 ggaactgttg ccacttggta cgcgcacctg aatagtccgc gcatctaacg cggtggcgct
    73021 gccatatcca cgcacgcggt tgatggtgtc agcgatttgc tgcgccatgc tgaaatcttc
    73081 gtcgttaagt tgcaaattaa gggtattccc gacgccaaac tggctgggca attcacgttc
    73141 aataaccgca ccattggtga tccgtccacc gttcagttgg ttaacctgaa cactgctacc
    73201 gcctgcggag gctcctgcgc cgccaaccag aatattgccc tgcgccagcg catacacctg
    73261 actgtcaacg cccttaagcg gtgtcatcaa caacgtacct ccacgcaagc ttttggcatt
    73321 acccatggaa gaaaccacca catcgatggt ttgcccctga cgtccaaacg gaggaagtga
    73381 cgctgtcacc attaccgcag cgacgttttt tagctgcata ttggtgcccg tcggaacggt
    73441 aattcccagc tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg tctgggttgt
    73501 ctggtcacca gtgccatcca gccccactac caagccatag ccaatcagtg agttttgcct
    73561 taccccctga acactggtga gatcgcgaat acgctcagcc tgagccgccg tcgtaaccag
    73621 tagaagaatt aatgcagaga gaaatttaat caccacagcc tcacttacat tggcgacagg
    73681 ttaaggaaga aacgctgcaa ccagcccata ttttgcgctt cgttaatgta gccattgcct
    73741 acgtattcaa tacgcgcatc cgccacctga gtagacggta cggtattgct gccgctgata
    73801 gtgcgtggat taaccacgcc cgagaagcga ataaattcgg taccctgatt aatggcaatc
    73861 tgtttttcac ccaccacatg caggttgccg ttgaccagta cctggtcaac cgtcaccgtc
    73921 aacgtgccgc taaaggtatt gctggcattg gccccgccct ttccgttgaa cgtgttacca
    73981 ccggaggctt cgacatcggc acgagcgtta ccaaacagcc cctgcaaata gcgcggcaca
    74041 gtatcaaagc caaaattagt tttaccgtca cggctggcat tcgcagagga gcttttgctg
    74101 gcgctgacgt tctcctgcaa cacgatggtc agcgtatcgc caatattgcg tggtcgacga
    74161 tcttcaaaca gcggttgata gccatagtta atcggctgag cagactggaa aatagaaccg
    74221 ttggcgacgg gcgtcggacc gggaaccggt tgtgcactgg tcgccccctg caccagcggc
    74281 gtggagggta tccaggcgca gccggttagt gaaagcacca acaagctgga aatggcataa
    74341 gtatgcgcag cgtttttttg catggctatc atcttcaaaa atcagtaaac cggtgaacct
    74401 gccaccggtt atgccttaga gttgcgtcag tttttgcagc atctgatcgg tggtggacac
    74461 cgctttactg ttgatttcgt aagcgcgttg cacctgaatc atattgacca gttcttccgc
    74521 cacgttgacg ttagacgttt caacataccc ttgatacagc agtcccgcgc cgttcaggcc
    74581 cggcgtgctt tcgttcggtg caccggagga ttgcgtttcg gtgtagaggt tttcgccaat
    74641 gctctccagt ccggtatcat tcataaaggt ggtgagattg agctgcccaa cctgaaccgg
    74701 agctgcctgg ccttgttggg ttacgctgac cacgccatca cgaccgatgg tgatacttaa
    74761 cgcattcgcc ggaatggtga tcgctggctg cacctgaaaa ccaccagccg tcaccagctg
    74821 cccgttctga tccacctgga aagagccgtc acggatatag gctgatgagc catctggcaa
    74881 cattacctgg aaaaagccct gccctttaat cgcgacatct ttgctgttat tggtctgcga
    74941 cagattgccc tggctgtgta agcgttcagt ggcgaccggg cgtacccccg tgccgatttg
    75001 taatccggag ggtaaggtgg tttgttcgga agactgtgcc cccggctggc gaatggtttg
    75061 ataaagcaga tcttcaaaca ccgcgcgctg acgcttaaaa ccgttagtac tgacgtttgc
    75121 caggttgttg gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg ttttggcgat
    75181 ccataatgaa ctgatcatat aatgtcctgt agtggattgc tgacaagcag agcagaagca
    75241 cgcgacagtg tgaatttccc ctcaccctaa ccctctcccc aaaggggcga gggaatagtc
    75301 cgcgcgcttt tattgcaatg tcatcaatcg ttcaattaac tcatcgacag cagttggttg
    75361 gcacggcctg cgttatcatc gacgctgctg atcaccttca tctgcatttc aaaacgccgc
    75421 gcgctggcaa tcatgtcgct cattgccgca acggcattga cgttactgcc ttccagaacc
    75481 cccgacatca cacgcaaggt tggatctgcc tgcagtaccg gccctcgcgt ggcctgggtt
    75541 tctgcgctta aacgaaaaat gccgtcgtca ccgcgctgca cttcgctgcc cgtggctttc
    75601 accagtttca gacgccctac tggcgcaact gtatttgccg gatcgcccgg attgagcgcc
    75661 gagattgtgc catcggcagc aatcgtgatt tccgcccctt ccggcacagc aattggcccc
    75721 gcctcgccta tcaccggatg cccctgaatt gtcagttgcc cggtgggatc aacctgaatg
    75781 ctgccattac gcgtataccc ttcgctgccg tcagcggtct gcacggccag ccatcctgct
    75841 gcaacgcaac gtccagcggg cgcgaggtgt aatccatctt gccgggcgtc atgtctgcac
    75901 ccggtgttga cgccgttacc agcgtgcgcg tgggcagtga aagcccttcc accggcactg
    75961 cgcgcaaggc gtttaactgc gcgcgaaaac cgggcgtaga ggcattggcc agattactgg
    76021 cggttaccgc ctgttgattc agcgtctggc tggctgctcc catcgcagta taaattgcgt
    76081 gatccattga gctatcccgt cagcgattag cgtaagttaa cccgcgtgtt gaggatctgg
    76141 tcctgggttt tgatggtctg ggcgttagac ttatagttac gctgggcaac gatcatattg
    76201 accagttctt tactgagatc gacgttggac gcttccagcg caccgttggt cagggtgcca
    76261 aagtttcccg tcccggctgt ccccaacagc gccacgccag aagattgcgt cgcagaccag
    76321 acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa agttcgccag tacaatctgc
    76381 cccagcagtt gggtttgttc gttggaattg ttgccgacaa ccgtaccgtc atcattgatt
    76441 tgataactca ccagatcgcc cggtttgtag ccattctggg tggttgccac aatgttgtta
    76501 gcgccggtat tttgctgcat ggagttgagg aagctcagac taaacgtggc gggttctgca
    76561 ccgttaattg cgccggttgc gatattattc gccatcgcac catccactaa tgtgccatta
    76621 gcattaaatt ccagtgttgt cgctgtcttc gcaatgctgt ttggatcact gctatcctgg
    76681 gtgtagacct gccagttatt atccccggtc ttcacaaaat agacgctcat gtcatgagca
    76741 ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc cgcattgctg
    76801 gcgctgaatg gcgtaacagt aggaagcgga tcactggaat tcaggttgat ctgcatcgac
    76861 gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatatt ggtcggattc
    76921 gccccttgct gaatagtcgg cggcgtaccg gttaccgggt aacccgtcag ctgtaaacct
    76981 tgcgtattca acaggttacg gttttcatcc agcttaaatt gtccgttacg gctgtagaac
    77041 accgaaccgt tgctgtctac cagacggaaa aaaccgttct ggctgatagc aacgtccaga
    77101 cctcgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt
    77161 acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt gcctgattta
    77221 aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt ggtggcggca
    77281 gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa tgtgaaggct
    77341 taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag atccagcgta
    77401 ttaccgctgt tgccgcggat cacaccctgc accagagcaa actgcagcgg ctgggcaacc
    77461 agttgtgtac caccgttact ggcgctaatc gctacattgt aagaaccgtt cggcgcagtg
    77521 ctgccatcag tcaacgaacc gtcccaggtg aaactgtgaa ctccggcggt cagttcacca
    77581 atatcaatgg tgcgcacaac cgcgccattt ttatcggtga tggtggccgt aactttgtct
    77641 gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc ttcttcactg
    77701 ccggttcccg caagaacagt tgtgccgggg atcatcacgc cgtgaccgat caggttactg
    77761 gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt ggtattgagt
    77821 ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg acgtcagctc gttgttttcc
    77881 attggattgg tcgggtcctg gtttttcagc tgcgccacca gcaaagtcag aaaactgctt
    77941 tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact gacgccggta
    78001 tttgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat tgaccgagcg
    78061 taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg gcctgatagc
    78121 tgcgtgacgc cgacatggtg ttaaccatct ccccgacgac atcaacgttc ggcattttta
    78181 cgtagccctt tgcatctgcc agcggattac ctggttcata aaccagtttg tccggggcct
    78241 gactttctta acatcggcaa cctttacgcc gcctgtcgca gcacctggtg cagcgttaac
    78301 ctggaatacc acctgttttg cccgatatgg ctgtccatca ggaccggtca cgctatcagc
    78361 atttgccaga ttactggccg ccacgttcag gcgctgggac tgggcagtta acgccgaccc
    78421 ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta aaacgttcat
    78481 catgcctttg atttgcccgc tcaacgcgct aaggctcatc tggtattgca ggctgttatc
    78541 ggcaaactgg gtgcgttcgc gatccatatc gacggtatta ccgtcaagcg aaggctggtc
    78601 cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc gcctgcgccg ggatgtgttg
    78661 cgttgaggtc atcgtcagtg caaccacact ggttgcatcc cgtccacgtt gcatgacttt
    78721 tttaagttca ctggcaaaat cgatatcgcg cgcctgataa ccaggggtat cggcattggc
    78781 gatgtttgct gccagcactt cctgacgctg ggcgcgcaga ttgagcgcct cttgttgaaa
    78841 acgtaaggcg gcgtcgagct tatcgagcat atctcctccg caggtatcaa aattctgcca
    78901 tcagcttaaa tgcctttacg cgcgcgttat cggcggaata aacgcaaaat gggtcgctat
    78961 ttatgccgtt gatggtcatt gcggacaggt acaattcacg ttgtagaaat ggctgggggc
    79021 gaaaatgctg acaataaaac gtagcgtggc gatcatcgcg atactgttca gtccgttaag
    79081 tacggcgagc aatctcacat cgcaattgca caactttttt agcgcccaac tcgcgggggt
    79141 aagtgatgag gttcgtgttt ctattcgtac agcgcccaat ctactaccgc catgcgagca
    79201 gccattgctt tcgatgagca ataattcccg cctgtggggc aatgtgaatg tgttggcacg
    79261 ctgcggtaac gacaaacgat atttacaggt taatgtgcag gccacaggaa attatgtggt
    79321 tgccgcgatg cctattgtgc ggggaggaaa gctggaagct ggcaatgtca aactgaaacg
    79381 tggacgcctg gataccctgc caccgcgtac ggtgctggat atcaatcaac ttgttgatgc
    79441 cgttagcctg cgcgatctat cacccgatca acctatccag ttaacccagt ttcgccaggc
    79501 atggcgggta aaagcgggac aacgcgtcaa tgtgatcgcc agcggtgatg ggtttagcgc
    79561 caacgcagaa ggtcaggcgc tgaacaatgc agccgtcgca cagaatgcgc gggtgcgcat
    79621 ggtatcggga caggtagtca gcggcgttgt tgatgcagat gggaatattc ttataaacct
    79681 gtaagctgtt aaagattacc cgtcccttgc cgataaataa gcaacacatg ataaaagcgc
    79741 cctcaatgag gaataaacca tgagtattga tcgcacttcg cctctgaagc ctgtaagcac
    79801 cgttcaaccg cgcgaaacca ccgacgcgcc ggtaacgaac agccgggcgg caaaaacaac
    79861 cgcctccacc agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga tgcaacccgg
    79921 cagcagtgat atcaatcttg aacgtgttga agcgttaaaa ctggcgattc gtaacggtga
    79981 actaaaaatg gacaccggca aaattgccga tgcgctgatc aacgaagcgc agcaagactt
    80041 gcagagtaac tgatcgtatg acacgtcttg cagagatcct cgaccagatg tccgctgtgc
    80101 ttaacgatct caaaacggta atggatcaag agcagcaaca tctctctatg gggcagatca
    80161 acggcagcca gttgcaatgg attacagaac aaaaaagctc actgctggcg acgctggatt
    80221 acctcgaaca gttacgcaag aaagaaccca atacagcaaa tagcgttgat attagtcaac
    80281 gctggcagga aattactgtg aaaacgcagc aactacgcca aatgaatcaa cataacggct
    80341 ggttactgga aggacagatt gagcgcaatc aacaggcgct ggaaatgttg aaaccgcatc
    80401 aggaaccgac gctatatggg gcgaacggtc agacctcaac accccatcgc ggcagtaaaa
    80461 agatttcgat ctgaatatta tctccggcct gcactgcagg ccggaatgca ttgttacacc
    80521 gtccggcggg caaattcttt aactttgaag cccagtaccg ccagtgcagc gaagtacgcg
    80581 gcaatccccg ccagcacgac cgccattaaa cgcagtaagc gccagggcat ggtacccaat
    80641 gaccactccg gcatgatatg taacatacct aaaagcacgc cagacatcac cagtaccgcc
    80701 accaccagac gcaacagaaa cgccatccag ccgggttgcg gagtaaagat tttctgctta
    80761 cgcaactgcc agtaaagcag cgaagcattc agacacgccg ccagaccaat agaaagtgac
    80821 agcccggcat gtttcaacgg accaataaac gccaggttca tcaattgcgt taaaatcagc
    80881 gtaacgatgg caattttcac tggcgtttta atgtcctggc gggaataaaa gccaggagcc
    80941 aacactttca ctacaatcag gccaatcaaa cccaccgagt aggcaattaa cgcccgctgg
    81001 gtcatcagcg catcaaacgc ggtaaattta ccgtactgga acagcgaaac ggttaacgga
    81061 cctgaaagaa tgcccaacgc aaccgcactc ggcagtgcca acaggaaaca aagacgcaac
    81121 ccccagtcca tcaaacggtt gtattcatca tgattgccac tggcaaaact tttcgacagc
    81181 gacggcagca aaatggtgcc aagcgccacg cccagcacac cggagggaaa ctccattaag
    81241 cggtcggcgt aatacatcca agacaccgaa ccggaagcaa gaaacgaggc aaaaatggtg
    81301 ttgatgatta aggagatctg gctcacagag acgccaagga tcgccggtcc catctgtttc
    81361 accacgcgca ttgctccagc atcgtggaag ttaatgcgcg gcaggaccag catgccgatc
    81421 ttcttcaggt gcggtagctg atacaccagc tgcaggatgc cgccgaccgt aacagcccac
    81481 gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa accaatcatg
    81541 ctgatgttaa gcagtgttgg cgcaaacgcc ggaatcgaga agcggttcca cgtattcaga
    81601 atcgctccca ccagcgacgc cagggagatc agcaagatat agggaaaggt aatctttagt
    81661 agctggctgg tcagggcaaa tttgtcagct gtgtcagcga agcctggcgc ggtcaccatg
    81721 atcacccacg gtgcggcgag catgccagcg accgtcacaa ccgccagcgc aagtgtcagc
    81781 aggccagaaa cataagagac aaagacccgc gtggcgtctt caccctgctt acttttatat
    81841 tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat acggcgtaac
    81901 aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt
    81961 ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg aaaacatggt catcgagctg
    82021 acggcggcca gcgattttaa taaattcatc ggtgttctaa tccggaccca cgggcaaaac
    82081 gcctgcaagg caggcgctga aagaggcgaa tagtctacct agattatggt gaattgctac
    82141 cgccagatgt tacagggttt attcactcat cgcatcgcgc cagatcttgt caacgatacg
    82201 ttgcgccagc actgcctatt cgccggcggt ttgcggaact gtctgatttt gcacgcattc
    82261 aatgaagtgc cgcgcacagc cgacaaatcc acgttgctca agagtgctct gccaaccagg
    82321 aatcggttta ttcactacac cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat
    82381 gtcgatgagc gcaccgtcag tcacggcctg cacggtttca cgctgacttc cggcacggcg
    82441 atgcatacaa gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg caaacagcat
    82501 ttcgccagcg tcgttagtca gtagcgtacc gccatccaga gaggctttgc cgcccgacaa
    82561 ccacagcgcg gtatccacca catgcagata atcatccagc aacgtgaaat aaagatcgtg
    82621 tggcccgacg ctattgctac gatgtttatc cattcttagc gaggctgcgg tggcgagttg
    82681 cgtttttaac tcaccgtaga gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag
    82741 ttttttccgc gccgccagtt ccaccagcct ttcagcatcg cgcagatttt ctgccagcgg
    82801 tttatcgaca cagacatgta cccccgcatt gagtaacgta ctgaccacgt caaagtggct
    82861 ggcggtgctg gagtgcacaa aaaccgcatc acaactggcg gcaaggctgg ataacgaatc
    82921 ggcataagga atgcgccagc tgtcacaaat tggcagggct ttggcgcgcg taggcgacca
    82981 ggctccttgt aacgtccagt cagacgctgc cgccagcacc ggtaaccacg ctttttgcgc
    83041 aatgccgcct aatcccacta cgccgatacg taattttttc actttaatct cccagatggg
    83101 ccagcaacga ataaagacgt tgtttcagtt ctgccacttc gatttccagg gcttcgacgc
    83161 gggtctgtaa atcaccgtca accatgttcg acatagccgt taccgccggc tgatcttcaa
    83221 cctcaccact gaaaagatgc atgtagcggc tttcgcgttt acccggttcg cgggccagac
    83281 gcaccacaaa aggaccatct tcgcgattcg ccagttgttc cagcgtcgac tccacttccg
    83341 ccatatcgct gaattcatac attcgcgcgg cgcggctgcg cagttcaccc ggcgtctggg
    83401 caccacgcaa taacaacgtg gtgattaacg ccacttctgc ggcgctcagt ttcagatcgc
    83461 caaattctga attacaaaaa cgttgctcat atttggtgac ccgattacca aaaccgctca
    83521 ctgtgcgtag ataatgacgt ttgaccagat tatccagctg ttcctgcact tcggattcac
    83581 tcagattcat gaccggttca cggttcgttt tctgattaca ggccgtgact acaccattga
    83641 ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa ccaattacgc
    83701 gagcttccag ggcagtaagt tgatatttca tgagattctc cttagcggcc gggcgtccag
    83761 tctggggtag ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat caacagatag
    83821 tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta
    83881 ttgtgcggca tataattagc cataatgcga tgaatatgtt gggtgcgctg catataacga
    83941 atggctgcgg tcagggcttc aaacatgagt cctttgccct gccatttttg cccaatcgaa
    84001 taaccgagat agcaggcatg aaaagagccg cgaacaacat tggaaaaatt ggcaacgcca
    84061 ataatctctt tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc ttgtttatga
    84121 aattcgttaa tcatccctag cctggcctgc cagcctgatg gataacagtg gctttcgtcg
    84181 cgcactggct cccagggctt gaggaaatgg cgattctctg cgtaataatc cgccagacgc
    84241 caggcatcac gatcatgcac cagacgcacg accagtcggt ctgtggttaa gcgcactttt
    84301 ggcacgttac tgcgatagcc aaacattcta tacctactcc ttcccgtaac gtctttaata
    84361 cgctgtaagt attactatac ctgcgcaacc agaccctgtg aaaagtccaa aatgccattt
    84421 tctgcattat tcacatattt gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa
    84481 tattgtcccc gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa agtgctcatc
    84541 ttttatttcc cgggagggga aatgtcccgc gtatcgcagg cgaggaacct gggtaaatat
    84601 ttcctgctca tcgataatat gctggtcgtg ctggggttct ttgttgtctt cccgctgatc
    84661 tctatccgct tcgttgatca aatgggctgg gccgccgtca tggtcggtat tgctctcggt
    84721 ctacgccagt ttattcagca aggtctgggt attttcggcg gcgcaattgc cgaccgcttt
    84781 ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg ccgccggatt cgccacaatg
    84841 ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc tgctctcggg actcggcggc
    84901 acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac taatccgtcc acaacaacgt
    84961 ggtcgttttt tctcgctgtt gatgatgcag gacagtgcca gtgcggtcat tggcgcattg
    85021 ttggggagct ggctgttgca atacgacttt cgcctggtct gcgccacagg ggccgttttg
    85081 tttgtgctat gtgcggcgtt caatgcgtgg ttgttaccgg catggaaact ctccaccgta
    85141 cgcacgcccg ttcgcgaagg catgacccgc gtgatgcgtg acaagcgttt tgtcacctat
    85201 gtcctgacgc tggcgggtta ctacatgctg gctgtacaag tgatgctgat gctgccaatt
    85261 atggtcaacg acgtggctgg cgcgccctct gccgttaaat ggatgtatgc cattgaagcg
    85321 tgtctgtcgt taacgttgct ctaccctatc gcccgctgga gtgaaaagca ttttcgtctg
    85381 gaacaccggt tgatggctgg gctgttgata atgtcattaa gcatgatgcc ggtgggcatg
    85441 gtcagcggcc tacaacaact tttcaccctg atttgtctgt tttatatcgg gtcgatcatt
    85501 gccgagcctg cgcgtgaaac cttaagtgct tcgctggcgg acgcaagagc tcgcggcagc
    85561 tatatggggt ttagccgtct gggtctggcg attggcggcg ctattggtta tatcggtggt
    85621 ggctggctgt ttgacctggg caaatcggcg caccagccag agcttccgtg gatgatgctg
    85681 ggaattattg gcatcttcac tttccttgcg ctgggttggc agtttagtca gaaacgcgcc
    85741 gcgcgtcgtt tgctggaacg cgacgcctga tttgatactc ccccctgact cctccatact
    85801 gaaagtgaac tgacagccaa atggaggagt cacgtgaagc tatacattta cgatcactgc
    85861 ccttactgcc tcaaagcccg catgattttc ggcctgaaaa atatccccgt cgaattacat
    85921 gttctgctca acgacgacgc agaaacaccc acccggatgg tcggtcaaaa acaggttccc
    85981 attctgcaaa aagatgacag ccgctatatg ccagaaagca tggatatcgt tcactatgtc
    86041 gataaactcg acggcaaacc gttactgacc ggcaaacgtt cccctgccat tgaagagtgg
    86101 ctgcgcaagg tcaatggcta cgccaacaaa ctgctgttgc cgcgttttgc caaatcggca
    86161 tttgatgagt tttctactcc cgccgcgcgc aaatatttcg tcgacaagaa agaggccagc
    86221 gcgggtaatt ttgccgacct gctggcccac tctgacggtc tgattaagaa tatcagcgat
    86281 gatttgcgtg cgctggacaa actgatcgtc aaaccgaacg ccgtgaatgg cgaactttcg
    86341 gaagatgata ttcagctatt tccgctactg cgtaatctga cgctggtagc cggaatcaac
    86401 tggccaagcc gcgttgctaa ttaccgcgat aatatggcga aacagacaca aatcaatttg
    86461 ttatcatcaa tggcgattta atcctgaccc gccgcggcag cctgtattcg cgggttgccg
    86521 ccgttttcag ttttttctgc tggcgagttt atcgctggtg acacatgctg cgttgtgtca
    86581 ggatattgac gcagatttca tgtcgaggac taccatgaac aagtttttat ttgccgctgc
    86641 attgatcgtc agtggcctgc tcgttggctg taatcaactc acccaataca ccatcaccga
    86701 acaagaaatt aaccagtcgc ttgcgaaaca taataatttc tcaaaagata tcggtttacc
    86761 cggcgtggct gacgcccata ttgttctgac aaacttgacc agccaaattg gtcgcgaaga
    86821 gccgaataag gttaccctaa ccggagacgc caatctggac atgaactccc tgttcggtag
    86881 tcagaaagcg accatgaaac tgaagctgaa agcgctgccc gtgtttgata aagaaaaagg
    86941 tgcgatcttc ctgaaagaga tggaagtggt cgatgcgacg gtacaaccgg aaaaaatgca
    87001 aacggtgatg caaacgttgc ttccctattt gaaccaggca ttacgcaatt actttaacca
    87061 gcaacctgct tacgtcctgc gcgaagatgg cagtcagggc gaagcaatgg cgaaaaaact
    87121 ggcgaaaggc attgaagtga agccaggcga aattgtcatt ccgtttacgg attaatgacg
    87181 agggcgcatt cgcgcccttt atttttcgtg caaaggaaaa cgtttccgct tatcctttgt
    87241 gtccggcaaa aacatccctt cagccggagc atagagatta atgactgcac catcccaggt
    87301 attaaagatc cgccgcccag acgactggca ccttcacctc cgcgatggcg acatgttaaa
    87361 aactgtcgtg ccgtatacca gcgaaattta tggacgggct atcgtaatgc ccaatctggc
    87421 tccgcccgtg accacagttg aggctgccgt ggcgtatcgc caacgtattc ttgacgccgt
    87481 acctgccggg cacgatttca ccccattgat gacctgttat ttaacagatt cgctggatcc
    87541 taatgagctg gagcgcggat ttaatgaagc cgtgttcacc gctgcaaaac tttacccggc
    87601 aaacgcaacc actaactcca gccacggcgt gacgtcaatt gacgcaatca tgccggtact
    87661 tgagcgcatg gaaaaaatcg gtatgccgct actggtgcat ggtgaagtga cacatgcaga
    87721 tatcgacatt tttgatcgtg aagcgcgctt tatagaaagc gtgatggaac ctctgcgcca
    87781 gcgcctgact gcgctgaaag tcgtttttga gcacatcacc accaaagatg ctgccgacta
    87841 tgtccgtgac ggaaatgaac ggctggctgc caccatcact ccgcagcatc tgatgtttaa
    87901 ccgcaaccat atgctggttg gtggcgtgcg tccgcacctg tattgtttac ccatcctcaa
    87961 acgcaatatt caccaacagg cattgcgtga actggtcgcc agcggtttta atcgagtatt
    88021 cctcggtacg gattctgcgc cacatgcacg tcatcgcaaa gagagcagtt gcggctgcgc
    88081 gggctgcttc aacgccccaa ccgcgctggg cagttacgct accgtctttg aagagatgaa
    88141 tgctctgcag cactttgaag cattctgttc tgtaaacggc ccgcagttct atggcttgcc
    88201 ggtcaacgac acattcatcg aactggtacg tgaagagcaa caggttgctg aaagcatcgc
    88261 actgactgat gacaccctgg tgccattcct cgccggggaa acggtacgct ggtccgttaa
    88321 acaataaaaa tcgtagcttc ctgttgtcat taggttattt tatctgtata aataaccagt
    88381 atattcaaca gggggctatt atgcgaattg aagtcaccat agcgaaaact tctccattgc
    88441 cagctggggc tattgacgcc ctggctggcg aactttcccg ccgtattcag tatgcgtttc
    88501 ctgataatga aggccacgta tcggtacgtt atgccgcagc gaataattta tcggttattg
    88561 gcgcaacaaa agaagataaa cagcgcatta gcgaaattct ccaggaaacg tgggaaagcg
    88621 ccgatgactg gtttgtcagc gaataatatg cagtgatttt tgtttttgcc ggatcgcccc
    88681 ggctttttta ttttacttac gaattttgag tacaaaattt ctacacttcc tcaaaataat
    88741 tgcacaatat gctaaaaaaa tatgaatgac atgctgtttt attgttcata taaatcttaa
    88801 aagcagaaat ttatctgttc ccccctttaa aaattcggtg aataccctta cttattggta
    88861 tactgaagtt gcttcagata aaaacgcatt gaacctcgaa taacgttgtc tagtaacacg
    88921 aattaggggg ccatgatgga aaaaaataat gaagtcattc agactcatcc gctcgtaggg
    88981 tgggacatca gcaccgttga tagctatgat gcgctgatgt tgcgtttgca ctaccagacc
    89041 ccaaataagt ccgagcagga agggactgaa gttggtcaga cgctctggtt aaccactgat
    89101 gttgccagac aatttatttc gatattagaa gcaggaatcg ccaaaattga atccggtgat
    89161 ttcccggtaa acgagtatcg gcgtcattaa catgacatga ccatccaccc aaaaggcacc
    89221 tcaccggtgc ctttaccatt tctgggttgt atatctgcat tcagttaatt actctgctta
    89281 aaccatgaga gcagagagaa aaaaatgaaa tacgatctca tcattattgg cagcggttcc
    89341 gtaggcgctg ccgccgggta ttatgcaacc cgcgccggtt taaacgtgct aatgaccgac
    89401 gcccatatgc caccgcatca acacggcagc caccacggcg atacgcgatt aattcgccat
    89461 gcttatggtg aaggcgaaaa gtatgtcccg ttggtcctcc gcgcgcaaac gctgtgggat
    89521 gaactttccc gccacaacga agatgatccc atttttgtac gctctggtgt cattaacctc
    89581 ggcccggctg actccgcatt tctcgccaac gtcgcccaca gcgccgaaca gtggcaactc
    89641 aacgttgaaa agctcgatgc gcaagggatt atggcccgct ggccagaaat acgcgtcccg
    89701 gacaactaca tcggcttatt tgagactgat tccggttttt tgcgcagcga actggcgatt
    89761 aaaacctgga tccaactggc gaaggaagcg ggctgtgcgc aactgttcaa ctgcccggtc
    89821 accgcaattc gtcatgacga tgatggcgta actattgaaa cggttgacgg tgagtatcag
    89881 gcgaaaaaag cgattgtctg cgcgggaaca tgggtaaaag acctgctccc ggagctgcct
    89941 gtccagcccg tacgtaaagt atttgcctgg tatcaggccg atggccgcta tagcgtgaag
    90001 aataaattcc cggcgtttac cggtgaactg cccaatggcg atcaatatta tggttttccg
    90061 gcagaaaacg acgcgttgaa gattggcaaa cataacggag gccaggttat ccattcagcg
    90121 gatgaacgtg ttccgtttgc ggaagtggcc agtgatgggt cagaagcctt cccgttcttg
    90181 cgcaatgtat tgccgggtat cggttgctgc ctgtacggcg ctgcctgcac ctatgataat
    90241 tcgcctgacg aagattttat tatcgatacc ctacccggcc acgataatac actgctcatt
    90301 accggcctga gtgggcacgg ttttaaattt gcgtcagttt taggggaaat agctgccgat
    90361 tttgcgcaag accaaaaaag cgattttgat ttgacgccat tcaggctttc ccgcttccaa
    90421 taatcatcat tacggcctcc agaatgaggc cgtttttaca ggagtattta atgcgtcggc
    90481 tgttgcacta tctcatcaat aatattcgcg agcatctgat gctttatctt tttctctggg
    90541 gattgttggc aattatggat ctgatctatg tgttttattt ttagttttcc gcactgacat
    90601 aaactgacaa atatttaatc tccctttcat ttagcgtgaa aaattgaatg agattattcc
    90661 tgcttctaca gcactcacca gacatctctg atgctgaacc cactaaactt atgaagattc
    90721 tttcattatt aattcaaaat tattgaaata aaaataaata tcattcaatt tgttattttt
    90781 gtgttgcaat tcattctccg acacgccatt ttctttctct cgcaaattcg tattggatgc
    90841 acttatgtca ttcacaaata cccctgaacg ctatggtgtt atatctgcgg ccttccattg
    90901 gctaagcgca attattgtct acggcatgtt tgctttaggt ctgtggatgg tcacgctcag
    90961 ttattacgat ggctggtatc acaaagcacc cgaactgcat aaaagcatcg gtattttgtt
    91021 aatgatgggg ctggttattc gcgttctgtg gcgtgtcata tctccaccgc cgggtccgct
    91081 gccgagttat tcgcccatga ctcgtcttgc cgcaaaagct ggacatctcg cattgtacct
    91141 attgctgttc gccatcggca tcagcggcta tctgatctca actgccgatg gtaaaccgat
    91201 cagcgttttt ggctggtttg acgtccccgc gacccttgcc gacgctggcg cacaggcaga
    91261 ctttgccggt gccctgcatt tttggctcgc gtggagcgtc gtagtactgt ctgttatgca
    91321 cggatttatg gccctgaagc atcatttcat cgataaagac gacactctga agcgcatgct
    91381 gggaaagtcg tcatctgact atggagtata aaaaatgaaa aaaagcctgc ttggtttaac
    91441 cttcgcgtcc ctgatgttct ctgccggttc agcggttgcc gccgattaca aaattgacaa
    91501 agaaggtcag cacgcctttg ttaacttccg catacagcat cttggctata gctggttata
    91561 tggcaccttt aaagatttcg acggtacttt tacctttgat gaaaaaaatc cggctgccga
    91621 taaagtgaat gtgacaatta acaccaccag cgtcgatact aaccacgccg aacgcgataa
    91681 acatcttcgc agtgcggatt ttctcaatac cacaaaatat ccacaggcaa cattcacctc
    91741 caccagcgtg aagaaagacg gtgacgaact ggatattacc ggcgatctga cgctgaatgg
    91801 cgtaaccaaa cccgtcacgc tggaagcgaa attaattggt cagggcgacg acccatgggg
    91861 cggtaaacgt gcaggctttg aggccgaagg caaaattaag ctcaaagact tcaatatcaa
    91921 gacagattta ggtccagctt ctcaggaagt ggatctgatt atttcagtgg aaggcgtaca
    91981 gcagaagtaa gagtgaaatg ttgccggatg gcacgcatcc ggcaatgata tttattctgt
    92041 tggatcagga atacccagtg ttgtattcag acgtccacga gacttattaa agatcttatt
    92101 gccattttca cgtcctgccc gacggcgtcg ctgttcttct ggcggtaacg cgctttcttc
    92161 gcagcaaatc tcactacaac aacctttgta tttttccgcg catactggac actgaataaa
    92221 cagcaggtgg cagccatcat ttttacagtt ggtatggctg tcgcacggcg cgccgcactg
    92281 gtggcaatgc gcgataatct catccgatat acgttcgccc atccgctcgt caaaaacaaa
    92341 atttttgcca ataaaacgca ccggcaagcc ctgctcgcgc gccttacggg cgtattcaat
    92401 aattccgccc tcgatatgcc agactttgtt gaatccgtta tgtttcatcc aggcactggc
    92461 cttttcacaa cgaatgccgc cggtgcagta catgacgatt tttttatctt tatgtgcctg
    92521 catcatctcg actgcttttg gcagctgctc acggaaggta tctgccggaa tttcgagcgc
    92581 gttttcaaag tgccccactt catactcata gtggttacgc atgtcgataa atagtgcatc
    92641 gggatcgtca agcatggcgt tcacttccgc cgcttgcaga tactcaccaa cattgctggc
    92701 atcaaagtga ggatcgtcaa taccgtcggc aacaatgcga tcgcgtacct tcatgcgcag
    92761 tacccagaag gatttcccgt catcatccaa cgcgatattc aggcgtaaac cctctaaagc
    92821 cgggtcgaag gcatagagct gcgcgcgaaa tgtttcaaca ttgctcgcag gtacgctgat
    92881 ttgcgcgtta atgccctcat gcgccagata cactcgccca aaaacattca gcgcggtaaa
    92941 cagctgatat aaagcgtcac gggtcgcctt aggatcggcg atgtggaaat acttgtaaaa
    93001 cgaaatggtg gttcgcggtt cgctctcagc caacattttg gcttttaacg cgtcgttgga
    93061 aatgcggttg tgtaacactg gcatggtgta cggttcctgc gagatgggaa agtaaaaatc
    93121 cgcggcatga tatagcaatt atcgataatt aacatccaca cattttacgc tacatttgcg
    93181 cattaaaaat tatttgttat ttacaagcgc ggcaatttcg cccagtcttc agccacaaat
    93241 tttggttgcg ggcgaaaaaa tgcgacaata catacaattg cccgtatagg ttgaaaaaca
    93301 ggattgatat gacgaatcta cccaagttct ccaccgcact gcttcatccg cgttattggt
    93361 taacctggtt gggtattggc gtactttggt tggtcgtgca attgccctac ccgattatct
    93421 accgcctcgg ttgtggatta ggaaaactgg cgttacgttt tatgaaacga cgcgcaaaaa
    93481 ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag cgaacaagaa cgccgtaaaa
    93541 tggtggtgaa gaatttcgaa tccgttggca tgggcctgat ggaaaccggt atggcgtggt
    93601 tctggccgga ccgccgaatc gcccgctgga cggaagtgat cggcatggaa cacattcgtg
    93661 acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat ccattttctg acactggagc
    93721 tgggcgcgcg gcagtttggt atgcaggaac cggggattgg cgtttaccgc ccgaacgata
    93781 atccactgat tgactggcta caaacctggg gccgtttgcg ctcaaataaa tcgatgctcg
    93841 accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa aggcgaagtg gtctggtacg
    93901 caccagatca tgattacggc ccgcgctcaa gcgttttcgt cccgttattt gccgttgagc
    93961 aggctgcgac cacgaccgga acctggatgc tggcacggat gtccggcgca tgtctggtgc
    94021 ccttcgttcc acgccgtaag ccagatggca aagggtatca attgattatg ctgccaccag
    94081 agtgttctcc gccactggat gatgccgaaa ctaccgccgc gtggatgaac aaagtggtcg
    94141 aaaaatgcat catgatggca ccagagcagt atatgtggtt acaccgtcgc tttaaaacac
    94201 gcccggaagg cgttccttca cgctattaaa tctcccatgc cggatgcttc agaatggcat
    94261 ccggcattac cacagcaaat ccccctgatt tagcgataaa agctctctgg attgcgcccc
    94321 ctggaagtcg ggcgcataat tagtgtgttt atcttttctt cttatgttca ccgcgcctgg
    94381 cgcaccaaca gcggattgct atgtcaccct gtgaaaatga cacccctata aactggaaac
    94441 gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg ggccgccttc agtctggtaa
    94501 tgcccttctt acccctctac gttgagcagc ttggtgttac cggccactcc gccctgaata
    94561 tgtggtccgg tattgtcttc agtataatgg gttcctaaac acatcatttt aggtattgaa
    94621 tatgagacgg gcaatcagct acatcagatt ttcaagtgaa cgacaactga aaggggactc
    94681 tgtacgccga caatctaagc tggttactga ctggcttgat aagaaccctg agttttactt
    94741 ggattcatcc ttgagcttta aagatttggg gaaaagtgct tttagtggta agcacctgaa
    94801 aggtggtcta ggtgactttt taaccgctat cgagaaaggg cttgttaaag ctggtgatac
    94861 gctactaatc gagagtttag accgtctgtc acgtcaggat attgatattg cttctgagct
    94921 attacgccgt atcttaaggg ctggtgtaga tgtagtcaca ctgagcgatg gagaacatta
    94981 cacacgagaa agtttaaaag acccgttagc cctgattaaa agcattctga taatgcagcg
    95041 ggcgcatgag gaaagtttga gaaagtctga gcgtgtccaa gctgcatgga accgtaaaaa
    95101 agaactcatc tccgaaggta tcaaggtatc taggcgttgt cctgcatggt taaggctgaa
    95161 tgatgaccgt cgaacattca caatcattcc tgataaagtg gaagttgtta aacgagcatt
    95221 tgacctgaga ttacagggtt taagcttttg ggctatcacc agaactttaa acgatgaagg
    95281 tcacttgtca ttgaatcaat acacacccaa acaaaagggg tggtctgata ctgctgtaaa
    95341 gaaactgctc agaaatcgtg ctgtgattgg atgtttcaca cctgctggac gtgaagaagt
    95401 tcagggttac taccccgcaa taatcagtga gtcactattc tatcgagtac agcaactgaa
    95461 cacagggcaa tatggtaggg cttctgtatc aagtaatccg ctatcagtca acttgttcag
    95521 gggtatcatc aa
//
LOCUS       DAOFMX010000004        75615 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.004, whole genome shotgun sequence.
ACCESSION   DAOFMX010000004 DAOFMX010000000
VERSION     DAOFMX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.004"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(424..630)
                     /locus_tag="QBA20_000368"
     CDS             complement(424..630)
                     /locus_tag="QBA20_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDQ6291043.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            943..1032
                     /gene="kdpF"
                     /locus_tag="QBA20_000369"
     CDS             943..1032
                     /gene="kdpF"
                     /locus_tag="QBA20_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDQ6291044.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1032..2705
                     /gene="kdpA"
                     /locus_tag="QBA20_000370"
     CDS             1032..2705
                     /gene="kdpA"
                     /locus_tag="QBA20_000370"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDQ6291045.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2728..4775
                     /gene="kdpB"
                     /locus_tag="QBA20_000371"
                     /pseudo
     CDS             2728..4775
                     /gene="kdpB"
                     /locus_tag="QBA20_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4784..5356
                     /gene="kdpC"
                     /locus_tag="QBA20_000372"
     CDS             4784..5356
                     /gene="kdpC"
                     /locus_tag="QBA20_000372"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDQ6291046.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5349..8029
                     /gene="kdpD"
                     /locus_tag="QBA20_000373"
                     /pseudo
     CDS             5349..8029
                     /gene="kdpD"
                     /locus_tag="QBA20_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8026..8703
                     /gene="kdpE"
                     /locus_tag="QBA20_000374"
     CDS             8026..8703
                     /gene="kdpE"
                     /locus_tag="QBA20_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDQ6291047.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8978..9082
                     /locus_tag="QBA20_000375"
     CDS             8978..9082
                     /locus_tag="QBA20_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HDQ6291048.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9393..11590
                     /gene="speF"
                     /locus_tag="QBA20_000376"
                     /pseudo
     CDS             9393..11590
                     /gene="speF"
                     /locus_tag="QBA20_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11587..12906
                     /gene="potE"
                     /locus_tag="QBA20_000377"
     CDS             11587..12906
                     /gene="potE"
                     /locus_tag="QBA20_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDQ6291049.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12962..14602)
                     /gene="pgm"
                     /locus_tag="QBA20_000378"
     CDS             complement(12962..14602)
                     /gene="pgm"
                     /locus_tag="QBA20_000378"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDQ6291050.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14628..15173)
                     /gene="seqA"
                     /locus_tag="QBA20_000379"
     CDS             complement(14628..15173)
                     /gene="seqA"
                     /locus_tag="QBA20_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDQ6291051.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15358..16122
                     /gene="ybfF"
                     /locus_tag="QBA20_000380"
     CDS             15358..16122
                     /gene="ybfF"
                     /locus_tag="QBA20_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDQ6291052.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16262..16555
                     /gene="ybfE"
                     /locus_tag="QBA20_000381"
     CDS             16262..16555
                     /gene="ybfE"
                     /locus_tag="QBA20_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDQ6291053.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16695..17225
                     /gene="fldA"
                     /locus_tag="QBA20_000382"
     CDS             16695..17225
                     /gene="fldA"
                     /locus_tag="QBA20_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDQ6291054.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17435..17521
                     /gene="uof"
                     /locus_tag="QBA20_000383"
     CDS             17435..17521
                     /gene="uof"
                     /locus_tag="QBA20_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDQ6291055.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17514..17960
                     /gene="fur"
                     /locus_tag="QBA20_000384"
     CDS             17514..17960
                     /gene="fur"
                     /locus_tag="QBA20_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDQ6291056.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18044..18370)
                     /locus_tag="QBA20_000385"
     CDS             complement(18044..18370)
                     /locus_tag="QBA20_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291057.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18407..19813)
                     /gene="chiP"
                     /locus_tag="QBA20_000386"
     CDS             complement(18407..19813)
                     /gene="chiP"
                     /locus_tag="QBA20_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDQ6291058.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20392..22056)
                     /gene="glnS"
                     /locus_tag="QBA20_000387"
     CDS             complement(20392..22056)
                     /gene="glnS"
                     /locus_tag="QBA20_000387"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDQ6291059.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22259..24205)
                     /gene="nagE"
                     /locus_tag="QBA20_000388"
     CDS             complement(22259..24205)
                     /gene="nagE"
                     /locus_tag="QBA20_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDQ6291060.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24538..25338
                     /gene="nagB"
                     /locus_tag="QBA20_000389"
     CDS             24538..25338
                     /gene="nagB"
                     /locus_tag="QBA20_000389"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDQ6291061.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25398..26546
                     /gene="nagA"
                     /locus_tag="QBA20_000390"
     CDS             25398..26546
                     /gene="nagA"
                     /locus_tag="QBA20_000390"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDQ6291062.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26555..27775
                     /gene="nagC"
                     /locus_tag="QBA20_000391"
                     /gene_synonym="nagR"
     CDS             26555..27775
                     /gene="nagC"
                     /locus_tag="QBA20_000391"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDQ6291063.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27823..28575
                     /gene="nagD"
                     /locus_tag="QBA20_000392"
     CDS             27823..28575
                     /gene="nagD"
                     /locus_tag="QBA20_000392"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDQ6291064.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28972..30636
                     /gene="asnB"
                     /locus_tag="QBA20_000393"
                     /pseudo
     CDS             28972..30636
                     /gene="asnB"
                     /locus_tag="QBA20_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
     gene            31015..31091
                     /locus_tag="QBA20_000394"
     tRNA            31015..31091
                     /locus_tag="QBA20_000394"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31049..31051,aa:Met,seq:cat)
     gene            31100..31184
                     /locus_tag="QBA20_000395"
     tRNA            31100..31184
                     /locus_tag="QBA20_000395"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31134..31136,aa:Leu,seq:tag)
     gene            31208..31282
                     /locus_tag="QBA20_000396"
     tRNA            31208..31282
                     /locus_tag="QBA20_000396"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31240..31242,aa:Gln,seq:ttg)
     gene            31317..31391
                     /locus_tag="QBA20_000397"
     tRNA            31317..31391
                     /locus_tag="QBA20_000397"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31349..31351,aa:Gln,seq:ttg)
     gene            31407..31483
                     /locus_tag="QBA20_000398"
     tRNA            31407..31483
                     /locus_tag="QBA20_000398"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31441..31443,aa:Met,seq:cat)
     gene            31532..31606
                     /locus_tag="QBA20_000399"
     tRNA            31532..31606
                     /locus_tag="QBA20_000399"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31564..31566,aa:Gln,seq:ctg)
     gene            31644..31718
                     /locus_tag="QBA20_000400"
     tRNA            31644..31718
                     /locus_tag="QBA20_000400"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31676..31678,aa:Gln,seq:ctg)
     gene            complement(31872..33047)
                     /gene="ubiF"
                     /locus_tag="QBA20_000401"
     CDS             complement(31872..33047)
                     /gene="ubiF"
                     /locus_tag="QBA20_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDQ6291065.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33193..34617
                     /gene="miaB"
                     /locus_tag="QBA20_000402"
     CDS             33193..34617
                     /gene="miaB"
                     /locus_tag="QBA20_000402"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDQ6291066.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34770..35810
                     /locus_tag="QBA20_000403"
     CDS             34770..35810
                     /locus_tag="QBA20_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDQ6291067.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35807..36274
                     /gene="ybeY"
                     /locus_tag="QBA20_000404"
     CDS             35807..36274
                     /gene="ybeY"
                     /locus_tag="QBA20_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDQ6291068.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36364..37242
                     /gene="corC"
                     /locus_tag="QBA20_000405"
     CDS             36364..37242
                     /gene="corC"
                     /locus_tag="QBA20_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDQ6291069.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37267..38805
                     /gene="lnt"
                     /locus_tag="QBA20_000406"
     CDS             37267..38805
                     /gene="lnt"
                     /locus_tag="QBA20_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDQ6291070.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39202..40110
                     /gene="gltI"
                     /locus_tag="QBA20_000407"
     CDS             39202..40110
                     /gene="gltI"
                     /locus_tag="QBA20_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDQ6291071.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40280..41020
                     /gene="gltJ"
                     /locus_tag="QBA20_000408"
     CDS             40280..41020
                     /gene="gltJ"
                     /locus_tag="QBA20_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDQ6291072.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41020..41694
                     /gene="gltK"
                     /locus_tag="QBA20_000409"
     CDS             41020..41694
                     /gene="gltK"
                     /locus_tag="QBA20_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDQ6291073.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41694..42419
                     /gene="gltL"
                     /locus_tag="QBA20_000410"
     CDS             41694..42419
                     /gene="gltL"
                     /locus_tag="QBA20_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDQ6291074.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42537..43472
                     /gene="rihA"
                     /locus_tag="QBA20_000411"
     CDS             42537..43472
                     /gene="rihA"
                     /locus_tag="QBA20_000411"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDQ6291075.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43556..45226
                     /gene="hscC"
                     /locus_tag="QBA20_000412"
                     /pseudo
     CDS             43556..45226
                     /gene="hscC"
                     /locus_tag="QBA20_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45286..46737)
                     /gene="djlC"
                     /locus_tag="QBA20_000413"
     CDS             complement(45286..46737)
                     /gene="djlC"
                     /locus_tag="QBA20_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HDQ6291076.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46734..47441)
                     /locus_tag="QBA20_000414"
     CDS             complement(46734..47441)
                     /locus_tag="QBA20_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDQ6291077.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47543..48097
                     /locus_tag="QBA20_000415"
     CDS             47543..48097
                     /locus_tag="QBA20_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDQ6291078.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48107..49534)
                     /locus_tag="QBA20_000416"
     CDS             complement(48107..49534)
                     /locus_tag="QBA20_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HDQ6291079.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49531..50238)
                     /locus_tag="QBA20_000417"
     CDS             complement(49531..50238)
                     /locus_tag="QBA20_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDQ6291080.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50402..51379
                     /locus_tag="QBA20_000418"
     CDS             50402..51379
                     /locus_tag="QBA20_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HDQ6291081.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51449..51931)
                     /locus_tag="QBA20_000419"
     CDS             complement(51449..51931)
                     /locus_tag="QBA20_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDQ6291082.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52166..54748
                     /gene="leuS"
                     /locus_tag="QBA20_000420"
     CDS             52166..54748
                     /gene="leuS"
                     /locus_tag="QBA20_000420"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDQ6291083.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54763..55344
                     /gene="lptE"
                     /locus_tag="QBA20_000421"
     CDS             54763..55344
                     /gene="lptE"
                     /locus_tag="QBA20_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDQ6291084.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55344..56375
                     /gene="holA"
                     /locus_tag="QBA20_000422"
     CDS             55344..56375
                     /gene="holA"
                     /locus_tag="QBA20_000422"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDQ6291085.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56377..57018
                     /gene="nadD"
                     /locus_tag="QBA20_000423"
     CDS             56377..57018
                     /gene="nadD"
                     /locus_tag="QBA20_000423"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDQ6291086.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNSHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57042..57653
                     /locus_tag="QBA20_000424"
     CDS             57042..57653
                     /locus_tag="QBA20_000424"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDQ6291087.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57913..58230
                     /gene="rsfS"
                     /locus_tag="QBA20_000425"
                     /gene_synonym="rsfA"
     CDS             57913..58230
                     /gene="rsfS"
                     /locus_tag="QBA20_000425"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDQ6291088.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58234..58701
                     /gene="rlmH"
                     /locus_tag="QBA20_000426"
     CDS             58234..58701
                     /gene="rlmH"
                     /locus_tag="QBA20_000426"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDQ6291089.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58732..60633
                     /gene="mrdA"
                     /locus_tag="QBA20_000427"
     CDS             58732..60633
                     /gene="mrdA"
                     /locus_tag="QBA20_000427"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDQ6291090.1"
                     /translation="MKLHNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPYEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60636..61748
                     /gene="mrdB"
                     /locus_tag="QBA20_000428"
                     /gene_synonym="rodA"
     CDS             60636..61748
                     /gene="mrdB"
                     /locus_tag="QBA20_000428"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDQ6291091.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61759..62847
                     /gene="rlpA"
                     /locus_tag="QBA20_000429"
     CDS             61759..62847
                     /gene="rlpA"
                     /locus_tag="QBA20_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDQ6291092.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            62986..64197
                     /gene="dacA"
                     /locus_tag="QBA20_000430"
     CDS             62986..64197
                     /gene="dacA"
                     /locus_tag="QBA20_000430"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDQ6291093.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64308..64571
                     /locus_tag="QBA20_000431"
     CDS             64308..64571
                     /locus_tag="QBA20_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDQ6291094.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64672..65313
                     /gene="lipB"
                     /locus_tag="QBA20_000432"
     CDS             64672..65313
                     /gene="lipB"
                     /locus_tag="QBA20_000432"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDQ6291095.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65572..66525
                     /locus_tag="QBA20_000433"
     CDS             65572..66525
                     /locus_tag="QBA20_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDQ6291096.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66734..67699
                     /gene="lipA"
                     /locus_tag="QBA20_000434"
     CDS             66734..67699
                     /gene="lipA"
                     /locus_tag="QBA20_000434"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDQ6291097.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67800..68003)
                     /gene="tatE"
                     /locus_tag="QBA20_000435"
     CDS             complement(67800..68003)
                     /gene="tatE"
                     /locus_tag="QBA20_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDQ6291098.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68132..68920)
                     /locus_tag="QBA20_000436"
     CDS             complement(68132..68920)
                     /locus_tag="QBA20_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDQ6291099.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69013..69396
                     /gene="crcB"
                     /locus_tag="QBA20_000437"
     CDS             69013..69396
                     /gene="crcB"
                     /locus_tag="QBA20_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDQ6291100.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69450..69659)
                     /gene="cspE"
                     /locus_tag="QBA20_000438"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69450..69659)
                     /gene="cspE"
                     /locus_tag="QBA20_000438"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDQ6291101.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69834..70394)
                     /gene="pagP"
                     /locus_tag="QBA20_000439"
     CDS             complement(69834..70394)
                     /gene="pagP"
                     /locus_tag="QBA20_000439"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDQ6291102.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            70983..72368
                     /gene="dcuC"
                     /locus_tag="QBA20_000440"
     CDS             70983..72368
                     /gene="dcuC"
                     /locus_tag="QBA20_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDQ6291103.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72409..72750)
                     /locus_tag="QBA20_000441"
                     /pseudo
     CDS             complement(72409..72750)
                     /locus_tag="QBA20_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73088..73597)
                     /locus_tag="QBA20_000442"
     CDS             complement(73088..73597)
                     /locus_tag="QBA20_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ6291104.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73760..74005
                     /locus_tag="QBA20_000443"
     CDS             73760..74005
                     /locus_tag="QBA20_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6291105.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73980..75611
                     /locus_tag="QBA20_000444"
                     /pseudo
     CDS             73980..75611
                     /locus_tag="QBA20_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18609 a        18987 c        19201 g        18818 t
ORIGIN      
        1 attggattag atatgttaac aattaattat tcttaatata tcaataaagt atatattact
       61 ctctttacta tctgttttta ttgcggtagc gatactaata acaaaattcg gaatttattt
      121 agatgctgga attagaaaaa gggatgacgg ctttctcacc ttaccctgaa gtttcacgaa
      181 agtgtgtaag gaagtatgca atctaactgg agatttctga tgatctagtc gcttagagcg
      241 acaatgaagg tgaccgcatc ctgggtagcc ctatgaaagg atactattga aacgtgatgc
      301 taaaacagtg atgaaatgaa taatacgatg gaaacgtctt ccatgttaat tatcatggaa
      361 gacgttgatg ttgaacacct aaaccagccc cctgatacga ggctggctta agcgcgcagg
      421 caatcagtaa aaatcaccgg ttgccttttc ggcctgatcc atccacaccg gtttatcgct
      481 ggttttcgac cagacgcggt gcaggtagct gtaaaagcgg gcgcggtctt tccagaaaag
      541 catgaaaggc agcgccagaa cgcccgctgc aaccgcatat gtacggcgta aaaagataag
      601 ccatgcagga tattctctgt agagttccat acttttctcc cccttttgta tctacccggt
      661 gaatagcacc ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc ttttgtgtaa
      721 ttttactact catccgacca cttatttttg cttattgatg gtttatttac attcacactg
      781 taattaaatt acacaaaagt taaattaata ctaaccatca gttaaatcat ggcctttgcc
      841 atttttatac tttttttaca ccccgtccgc agatttttgc gaaatctttg cagccagaat
      901 tctactcttc cggtatcact tttaggccac tggaggtgca ctgtgagtgc aggcgtgata
      961 gccggcgtgt tgctggtgtt tttattactg ggttatttgg tttatgccct gatcaatgcg
     1021 gaggcgttct gatggctgcg caagggttct tactgattgc cacgttttta ctggtattaa
     1081 tggtgctggc gcgtccttta ggcagcgggc tggcgcggct gattaatgac attcctcttc
     1141 caggtacagc gggcgttgaa cgtatactct ttcgtttacc tggcgtctct gaccatgaga
     1201 tgaactggaa gcaatatctt tgtgccattc tcggcctgaa catgctgggg ctggcggtgc
     1261 tgttttttat gttgctcggt cagcactatc tgccgcttaa tccacagcag ttgccggggc
     1321 tgtcgtggga tctggcgctg aataccgccg tcagctttgt cacgaatacc aactggcaat
     1381 cttatagcgg tgaaaccacg ttgagctatt tcagccagat ggcaggctta acggtacaaa
     1441 actttctttc tgccgccagc gggattgcgg tgatttttgc cttcatccgt gcgtttactc
     1501 gccagagcat gagcacactc gggaatgcct gggtcgatct gctacgcatc acgttatggg
     1561 tgctagtccc tgtggcgttg ttgatcgcac tgttttttat tcaacaaggt gcgcagcaaa
     1621 actttctgcc ctatcaggca gtgaataccg ttgaaggagc gcaacagctg ttacccatgg
     1681 gaccagtagc ttctcaggaa gcgatcaaga tgctcggtac taacggcggt ggcttcttta
     1741 atgccaactc gtcgcatccg tttgaaaacc caaccgcact gaccaacttc gtgcagatgc
     1801 tggcgatctt cttgatccca acagcgctgt gctttgcctt tggtgaagtg acgggcgatc
     1861 gccgccaggg gcgcatgttg ctgtgggcga tgtcagtgat ttttgtcatc tgcgtaggcg
     1921 tggttatgtg ggcagaagtt cagggtaatc ctcatctgct ggcgctcggc gcagacagca
     1981 gcatcaatat ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc ctgtttgcgg
     2041 tcgtgactac ggcggcgtcc tgtggcgcgg tgattgcgat gcatgattcg tttaccgctc
     2101 tcggcggcat ggtgccgatg tggctaatgc aaattggtga agtggtgttc ggcggtgtcg
     2161 gttctggtct ttacggcatg atgctgtttg tcctgctggc ggtgtttatt gccgggctga
     2221 tgattggtcg tacaccggaa tatctgggta aaaaaataga cgtacgcgag atgaaactga
     2281 ctgcactggc gattctggtt actccaacac tggtactgat gggcgcggcg ttggcgatga
     2341 tgaccgacgc cggacgtagc gccatgctta accctagccc gcatggtttt agcgaagtgc
     2401 tgtacgccgt gtcgtccgcc gctaacaaca acggcagcgc ctttgccgga ttaagcgcca
     2461 actctccgtt ctggaactgt ttactggcgt tctgcatgtt tgtcggtcgc ttcggggtga
     2521 ttatcccggt gatggcaatt gccggttcgc tggtgagtaa aaagagccaa cccgccagct
     2581 ccggcacgct gccaacgcac ggcccgctgt ttgttggcct gttaatcggc accgtgttgc
     2641 tggttggcgc actgaccttt atccctgccc tggcgcttgg tccggtggcg gaatatctct
     2701 cctgatgata ttgagtgagc actgaatatg agtcgtaaac aactggcgct attcgaacca
     2761 acacttgtcg ttcaggcgct gaaagaagcg gtgaaaaaat taaacccgca ggcgcaatgg
     2821 cgcaatccgg tgatgtttat cgtctggatc ggcagtctgc tgaccacctg tattagcatc
     2881 gcgatggcaa gcggtgcgat gcctggcaat gcgctgttta gcgcggcaat tagcggctgg
     2941 ctgtgggtca ccgtactgtt cgctaatttc gccgaggcgc tggcagaagg ccgcagtaaa
     3001 gcgcaggcca acagtcttaa aggggtgaaa aaaactgcct ttgcccgcaa gctgcgtgag
     3061 ccgaaatatg gcgctgcggc ggacaaagtt cctgccgatc aactgcgtaa aggtgacatc
     3121 gtgctggtag aagctggcga tattatcccc tgcgatggtg aagtcattga aggcggcgca
     3181 tcggtcgatg aaagcgccat caccggggaa tcggcaccgg tgatccgtga atccggcggc
     3241 gattttgcct ccgtcaccgg cggcacgcgt attctttctg actggctggt gattgagtgt
     3301 agcgttaacc ccggtgagac atttctggat cggatgatcg cgatggtgga aggcgcacag
     3361 cgacgcaaaa caccgaacga gattgccctg actatcctgc tgattgctct gaccatcgtc
     3421 tttttactgg caaccgccac gctgtggccg ttttccgcgt ggggcggtaa tgccgtgagt
     3481 gtaacggtac tggtggcgct gctggtctgt ctgatcccaa ccactattgg tggcctgttg
     3541 tcagcgatcg gcgtcgccgg gatgagtcgg atgctgggcg cgaatgtgat tgccaccagc
     3601 ggacgtgcag ttgaagcggc aggtgacgtt gacgttctgc tactggataa aaccggcacc
     3661 atcacactcg gtaaccgtca ggcgtcggag tttatccccg cgcagggcgt ggatgaaaaa
     3721 acgctggctg acgccgcaca actggcttcg ctggctgatg aaacgccgga aggtcgcagt
     3781 attgtgatcc tcgccaagca acgttttaac ctgcgcgagc gcgatgtgca gtcgctccat
     3841 gccacctttg taccgtttac tgcgcaaagc cggatgagcg ggatcaacat cgacaaccgc
     3901 atgatccgta aaggttctgt cgatgccatt cgccgccata ttgaggctaa cggcggtcac
     3961 ttccctgccg atgttgatca aaaagtcgat caggttgcgc gtcaaggagc cacgctgctg
     4021 gtggtggtgg aaggttctcg tgtgctgggc gttattgcgc tgaaagatat cgtcaaaggc
     4081 ggtattaaag agcgcttcgc ccagctgcgc aaatgggcat taaaacggtg atgattaccg
     4141 gcgataaccg tctgactgcc gccgcgatag ctgcggaagc gggtgtcgat gattttctcg
     4201 ccgaagcgac accggaggcc aagctggcat tgatccgtca gtatcaggcg gaaggtcgtt
     4261 tggtagcgat gaccggcgac agcaccaacg atgctacggc gctggcgcag gcagatgtcg
     4321 cggtggcgat gaactccggc acccaggcgg ctaaagaggc gggcaatatg gtggatctcg
     4381 actctaaccc gaccaagttg atcgaggtgg tgcacattgg caaacagatg ctgatgaccc
     4441 gtggctcgct gaccaccttc agcattgcca acgatgtggc gaaatacttc gccattattc
     4501 cggcggcgtt cgcggcaaca tatccgcagt taaatgcgct gaacatcatg cgcctgcatt
     4561 cgcccgactc cgccattctc agtgcggtca ttttcaacgc cttgattatc gtctttttga
     4621 ttcctctggc gttaaaaggc gtgagttata aaccgcttac cgtttctgcc atgttgcgcc
     4681 gtaacttatg gatttacggt ctgggtgggc tgctggtgcc gtttattggt atcaaagtca
     4741 ttgatttact gctaaccgtt tgtagtctgg tgtgaggttt accatgagtg gattacgtcc
     4801 ggcattatcg acatttctct ttctgttatt gattactggc ggcgtttacc cgctgctgac
     4861 caccgcactg gggcaatggt ggtatccctg gcaggccaac ggttcgttga ttcgtgaagg
     4921 tgatacggtg cgcggttcgg cattaatcgg gcaaaatttt accggcaacg gctattttca
     4981 tggtcgcccg tcggcaacag cagaaatgcc ctataatcca caggcttctg gcgggagcaa
     5041 tctggcagtc agtaacccgg agctggataa acagatagcc gcacgcgttg ccgcattacg
     5101 ggccgctaac ccggacgcca gcacgaacgt tccggttgaa ctggtgacgg cgtcggcaag
     5161 cggactggac aataacatca ccccgcaagc ggcggcctgg cagatcccac gcatcgcaaa
     5221 agcgcgtaat ctcagcgttg aacagctcac gcagctgatc gcaaaataca gccaacaacc
     5281 gctggtgaaa tatatcggcc agccggtcgt caacattgtc gaactcaatc tggcactgga
     5341 taagcttgat gaataacgaa cccttacgtc ccgaccccga ccgtctgctg gaacaaactg
     5401 ccgcgccgca tcgggggaag ctgaaagttt tcttcagtgc ctgtgcaggc gtcgggaaga
     5461 cctgggcgat gctggcagaa gcccagcgac tgcgggcgca agggctggat attgtggttg
     5521 gtgtggtaga aacccatggg cggaaagata ccgccgccat gcttgaaggg ctggctgttc
     5581 tgcctctgaa acgccaggcg taccgtgggc ggcatatcag cgagtttgat ctcgatgccg
     5641 ccctcgcccg ccgtccggcg ctgatcttaa tggacgaact ggcgcacagt aatgcgccag
     5701 gttctcgtca tcctaaacgc tggcaggata tcgaagaact gctggaagct ggcattgatg
     5761 ttttcacaac cgtcaacgtt cagcatctgg aaagtctgaa tgatgtggtc agcggcgtca
     5821 ccggaattca ggtacgggaa accgtgcccg atcctttttt cgatgccgcc gacgacgtgg
     5881 tgctggtgga cttgcccccg gacgatctgc gccagcggct gaaagaaggc aaagtctata
     5941 ttgccgggca ggcggagcgc gccattgaac attttttccg caaaggcaat ctgatcgccc
     6001 tgcgcgaact ggcactgcgc cgtactgccg atcgcgttga tgagcaaatg cgcgcctggc
     6061 gggggcatcc tggcgaagag aaagtctggc acacgcgcga cgcgatcctt ttatgcatcg
     6121 gccataacac cggcagcgaa aactggtccg cgcggcggcg cggctgacgt cacggctggg
     6181 gagcgtctgg cacgcggtgt atgttgaaac ccctgccctg catcgcttac cggagaaaaa
     6241 acgtcgggca attctcagcg ccttacgtct ggcgcaggag ctgggcgcgg agacggcaac
     6301 actttctgat ccagcggaag agaaagcggt agtgcgttat gcccgtgaac ataatctcgg
     6361 caagattatt ctcggtcgcc cagcctcgcg ccgctggtgg cgtcgggaaa catttgctga
     6421 ccgactggcg cgcatcgccc ccgatctcga tcaggtgctg gtcgcgcttg atgaaccacc
     6481 cgcccgcacg attaacaacg cgccggatag ccgctcattt aaagacaagt ggcgggtaca
     6541 gattcaggga tgcgtggttg ccgccgcgtt atgcgccgtt atcaccttaa ttgccatgca
     6601 gtggctgatg gcgtttgatg ccgccaacct ggtgatgctg tatctgcttg gcgtggtggt
     6661 ggcgctattt tatggacgct ggccttcggt ggttgccacc gtcattaatg tggtgagttt
     6721 cgatctcttt tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc aatatctgct
     6781 gaccttcgcg gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg ctggcgtgcg
     6841 ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca cggcacttat atgaaatgtc
     6901 gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc gccaccagcg aacaatttat
     6961 tgcctccacg tttcatgccc gcagtcaggt gttgttgccc gatgacaacg gtaaattgca
     7021 gccgttaaca catccgcaag gagttacgcc atgggacgat gccatcgcgc agtggagttt
     7081 tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac cgtaccagat
     7141 tttgccgcta aaaagcggcg agaaaaccta cgggctggtg gtggtggagc cggggaatct
     7201 gcgccagttg atgatcccgg aacagcagcg cctgctggag acttttacgc tgttagtcgc
     7261 caatgccctt gagcggctga cgctaaccgc cagcgaagaa caggcgcgga tggcaagcga
     7321 acgtgaacag atccgcaacg ccctgctggc agcgctttcg catgatttac gcacgccgct
     7381 tacggtgctg tttggtcagg cagaaatctt aacgctcgat ctggcaagcg aaggatcacc
     7441 ccacgcccgc caggccagcg agattcgtca gcatgtgctg aacactaccc gactggtgaa
     7501 taatctactg gatatggcgc gaattcagtc cggcggcttt aatttgaaga aagagtggtt
     7561 aacgctggaa gaagtagtcg gcagcgcgct gcaaatgccg gaaccgggtt tatcgtcgcc
     7621 catcaatctt tctctgccag aaccgctgac cttaatccac gttgacgggc tactctttga
     7681 acgggtgctg attaatctgc tggagaacgc ggtgaaatat gcgggtgcgc aggccgaaat
     7741 tggtatcgat gcccacgttg agggcgaaaa tctacaactg gatgtctggg ataacggccc
     7801 cggtcttccg ccaggccagg agcagacgat atttgataag tttactcgcg ggaataaaga
     7861 gtcggcggtg ccgggtgtcg ggcttgggtt ggcaatttgt cgggcgattg tggatgtaca
     7921 cggcggcacc attaccgcgt tcaaccgacc ggaaggtggt gcctgttttc gtgttacact
     7981 tccccagcaa accgctcctg aacttgaaga ctttcatgag gatatgtgac aaacgttctg
     8041 attgttgaag atgaacaggc tattcgtcgc tttctgcgca cggcgctgga gggcgacggg
     8101 atgcgcgtct ttgaggccga aactctgcaa cgcggcttgc tggaagcggc aacccgtaag
     8161 ccagatttga ttattctcga tctcggcctg cccgatggtg atgggattga gtttatccgc
     8221 gacctgcgcc agtggagccc ggtgccggtg attgtgcttt ccgcacgcag cgaagagagc
     8281 gacaagatcg ccgcgctgga tgccggagcg gatgattatc tgagtaagcc gtttggtatt
     8341 ggcgaattgc aggcccgtct gcgcgtcgct ttacgccgcc actccgccac cgccgcgccc
     8401 gatccgctgg tgaaattttc cggtgttacc gtcgatttag ccgcccgcgt gattcaccgg
     8461 ggtgaggaag aggtgcatct cacaccaatt gagttccgcc tgctggcggt gctgctcaac
     8521 aatgccggaa aagtactcac ccagcgccag ctccttaacc aggtgtgggg gccaaacgcg
     8581 gtcgaacaca gtcactattt gcgtatttat atgggacatc tgcgacaaaa actggaacag
     8641 gatccggccc ggcctcgcca cttcattact gaaaccggta ttgggtatcg gtttatgctt
     8701 tgaattgtta ttcaataaat tcgcttaaat agtgattaat ttattattaa acctgcgccg
     8761 aatattatta tttggcaggt ttaatttctt aacgcaaata taaaacacga aattacaaca
     8821 tttaagtaac aaaacagcat ctaatatggc gatattcata attaatgatt tattatctgg
     8881 ggtatgatcg ttttttgttg atcttcttca cagattatag ccatttcatg gatagaataa
     8941 ctctaccttc aactgacaca gcaagcggta aaggtaaatg gaaaataata accgcttaat
     9001 gcctcatata aggcggacaa cccatatcat gaagtttgcc catcgtaata gttttgactt
     9061 tcatttcttt aatgcccgct agtcttctga ctaaagggca ccccaacgta caggtctccc
     9121 tgactttaag cattacaggt taatacctgt attcctcggt gctcatatac tgctaaccct
     9181 tttaaactct aaataattcg agtcgcagca cttgcaactt gaggtatgac gagtatagcc
     9241 agttaccggg ctggtctggg ttattgcatc tgcaaaaagc aaactactga tttatttatc
     9301 agcggtggag ctttgctttt tttccgacgt gatcgatttc tcctttgaga aattgaggac
     9361 ctgctattac ctgaaataaa gagatgaata aaatgtcaga attaaaaatt gccgttagcc
     9421 gttcttgccc ggattgtttt tccactcatc gtgcatgcgt gaatattgac gaaagtaatt
     9481 atattgacgt tgccgccatt attttatcag tcaatgatgt tgaacgtgga aaactcgata
     9541 aaatagacgc tactggctat ggcattcctg tttttattgc aacggaaaat gaagaacgtg
     9601 taccagcaga atatctgcca cgtatttctg gtgtcttcga gcattgcgaa tcacggaaag
     9661 agttttatgg acgtcagtta gaaaccgctg ctagccatta tgaaacgcaa ctgcgcccgc
     9721 ccttcttccg cgcactggtc gattatgtca atcagggcaa tagcgctttt gactgcccgg
     9781 gtcatcaggg aggtgagttt ttccgtcgtc atccggcagg gaatcagttc gtggagtact
     9841 tcggagaaac gctgttccgt tccgacttat gcaacgcaga cgtagcaatg ggcgatctgc
     9901 tgattcacga aggcgcgcca tgcattgcac agcaacatgc ggcaaaagtg tttaatgccg
     9961 ataaaacata cttcgttcta aatggcactt catcttctaa caaagtggtt ttaaacgccc
    10021 tgctaacacc gggtgatctg gtgctgtttg atcgcaataa ccacaaatct aaccaccacg
    10081 gagcgttgct acaggctggt gcaacaccgg tttatctgga aacggcacgt aacccgtatg
    10141 gctttatcgg tggcattgat gcgcactgtt ttgaagaaag ttacctgcgt gagctgatcg
    10201 cggaagtcgc accgcagcgg gcaaaagagg ctcgtccttt ccgcctcgct gtgattcagt
    10261 taggcaccta cgacgatacg atttataacg cccgccaggt ggtggataaa attggtcatc
    10321 tgtgtgacta cattctgttt gactccgcat gggttggcta tgagcaattt atcccgatga
    10381 tggctgactg ttcgccgctg ttgctggatc ttaatgagaa cgatccgggt attctagtta
    10441 cgcaatccgt acataaacag caggctggtt tttctcaaac ttcacaaatc cataaaaaag
    10501 acagccacat caaagggcaa cagcgttatg taccgcacaa acgcatgaac aacgccttta
    10561 tgatgcacgc ctccaccagc ccgttctatc cgctgtttgc cgcactggat atcaacgcca
    10621 aaatgcatga aggtgtcagc ggtcgtaata tgtggatgga ttgtgtggta aatggcatta
    10681 atgcccgcaa actgatcctc gataactgtc agcatattcg tccgttcgta cctgaactgg
    10741 tggatggtaa accctggcag tcgtatgaaa cagcgcaaat tgcggttgat ctgcgcttct
    10801 tccagtttgt accaggggaa cactggcatt cttttgaagg ctatgcagag aatcaatact
    10861 ttgtcgatcc ttgcaaactg ttgctgacaa ccccaggtat tgatgcacgt aacggcgaat
    10921 atgaagcgtt cggtgtaccc gcgacgattc ttgctaactt cctgcgcgaa aatggcgtag
    10981 tgccggaaaa atgcgatctt aactccatcc tcttcctgct gactccggca gaagatatgg
    11041 ccaaacttca gcaacttgtg ccctgctggt acgcttcgaa aaactgcttg agtccgacgc
    11101 gccattagca gaagtgctgc cttccatcta caaacagcat gaagagcgct acgccggtta
    11161 taccctgcgt cagttgtgcc aggaaatgca tgatttgtat gcccgccaca acgtgaaaca
    11221 actgcaaaaa gagatgttcc gcaaagcgca cttcccacac gtcagcatga atccgcaaga
    11281 agccaactac gcctatttac gcggtgaagt ggagctggtt cgtctgccgg atgccgaagg
    11341 tcgtatcgct gccgaagggg cgcttcctta tcctccgggt gtgctgtgtg ttgttccggg
    11401 tgaaatctgg ggtggtgctg ttttgcgtta cttcagcgct ctggaagaag ggatcaacct
    11461 gctgccaggt tttgcaccgg agctgcaggg tgtctatatc gaagaacatg atggtcgtaa
    11521 gcaagtttgg tgctatgtca tcaagcctcg tgatgcgcaa agcaccctgt tgaaagggga
    11581 aaaattatga gtcaggctaa atcgaacaaa atgggcgtcg ttcagttaac catactgacg
    11641 atggtcaaca tgatgggctc cggtatcatc atgctgccga caaagcttgc cgaagttggg
    11701 acaatttcaa ttatctcctg gctggtgaca gccgtaggct caatggcact ggcatgggca
    11761 ttcgcaaaat gcggtatgtt cagccgtaaa tcaggtggta tgggcggtta tgccgagtat
    11821 gctttcggta aatccggtaa ctttattgcg aactatacct acggcgtctc actgctgatt
    11881 gctaacgtcg cgattgctat ttcggcggtt ggttacggca ccgaactgct cggcgcgagt
    11941 ttgtcgccag tgcagattgg tcttgcgacc atcggggtgc tgtggatttg taccgtggct
    12001 aactttggtg gtgcgcgcat taccgggcaa atcagtagca ttaccgtgtg gggggtcatt
    12061 attccggtgg taggcctgtg cattatcggc tggttctggt ttagcccgac gctttacgtt
    12121 gattcctgga atccgcatca tgcaccgttc ttcagtgcag taggttcttc catcgctatg
    12181 acgctgtggg cttttcttgg tctggagtct gcgtgtgcga atactgatgt agtggaaaac
    12241 ccggaacgta atgtgccaat cgcggtactc ggcggtacgt taggtgcggc ggtgatttat
    12301 atcgtctcca ccaacgtgat tgcagggatt gtgcctaata tggagctggc aaattcaacg
    12361 gcaccatttg gcctggcctt cgcacagatg tttacaccag aagtgggtaa agtcattatg
    12421 gcgctgatgg tgatgtcctg ctgcggttcg ctgcttggct ggcagttcac cattgcccag
    12481 gtgtttaaat cttcatctga tgaaggctac ttccctaaaa ttttctcccg tgtaaccaaa
    12541 gtggatgcac cggtgcaggg aatgttgacc attgtgatta tccagagcgg gttggcgctg
    12601 atgacgatta gcccgtcgct aaacagtcag ttcaacgtgc tggttaacct ggccgtggtg
    12661 accaatatca ttccgtatat tctgtcaatg gcggcattag tcattattca gaaggtcgct
    12721 aatgtgcccc cctcaaaagc gaaagttgca aactttgttg cttttgttgg cgcgatgtat
    12781 agcttttatg cgctgtactc atccggggaa gaagccatgc tgtacggttc catcgtgacc
    12841 ttccttggtt ggacactgta tggtctggta tcaccacgct ttgaactgaa aaataaacac
    12901 ggttaaaaaa gcaaagtaaa aaagggcgat cttgcgaccg ctcttttttt attaaatgtg
    12961 tttacgcgtt tttcagaact tcgctaacaa tctcaaccgc ttctttctca atctgcttgc
    13021 gatgttcttc accgaggaag ctttcgcagt agatcttata tgcgtcttcc gtgcctgacg
    13081 gacgcgcggc gaaccagccg ttgtcagtca tcactttcag accgccaata gaagcaccgt
    13141 tgcccggagc agcagtcagg cgcgcggtga tcgggtcacc tgccagggtg ctggcgctca
    13201 ccatttccgg agacagctta gacagcgccg ctttttgtgc ggaagtcgca gctgcctgca
    13261 aacggttgta gctcggcgca ccaaagcgtt ttgccagttc gttgtagtgt tcctgcgggt
    13321 tcttaccggt gacagcggtg atttccgccg ccagcagaca cataatgatg ccgtctttgt
    13381 cggtggacca cggcgtgccg tcgaaacgca ggaaggaagc ccctgcgctc tcttcgccgc
    13441 caaagccgaa gctgccgtcg aacagaccat caacaaacca tttgaaacct accggtactt
    13501 ctaccagctt acggcccaag tcattgacca cacggtcgat catcgcagaa gaaaccagcg
    13561 ttttaccgac agcaacatct ttgccccact gcggacgatg ctggaacagg tagttgatcg
    13621 ccaccgccag gtagtggttc ggattcatca aacctgccgg agtgacgata ccgtgacggt
    13681 cataatccgg gtcgttagca aacgccagat cgaacttatc acgcagtgcc agcagacccg
    13741 ccatcgcaca ctcggaggag cagtccatac ggatcgcacc gtctttatcg aggtgcataa
    13801 agcggaaggt ttgatcgacc tgatcgttaa cgatggtcag gttgaggttg taatactcgc
    13861 caatgcgctt ccagtattcg ataccggaac cgcccaatgg atcaacgccc agcgtcaggc
    13921 ccgctttctg aatcgccgcc atatcaacga tatcggccag cccttccacg aacggctgca
    13981 ccagatcctg ctctttcaca tagccggatg ccatcgcttc gtcgagggag atacgcttca
    14041 cgcctttcag gccatcggcc atcagtgcgt tggccctgtc ttccaccact ttggtgacgt
    14101 tagtatcagc cgggccacca tttggcggat tgtacttgat accaccatct tccggcgggt
    14161 tatgggacgg tgtaatcacg ataccgtctg ccagcgggcc acctttttta ttgtgaacca
    14221 ggatggcatt ggaaacggca ggcgtcggag tgaagccatt gttttcctgc acaatgacat
    14281 caacgccgtt cgctgccagc acttccagta cggaaatgaa cgcaggctcg gacagggcgt
    14341 gagtatcttt acccacatag caagggccag tgatgccgtt tttcgcacgt tcttcagcaa
    14401 ttgcctgagc gatcgccaga atgtgcggct cgttaaagct gtggcgcgct gcactgccac
    14461 ggtgaccgga agtaccgaat ttcaccgcgt gctccgcatt ccctgcttct ggtttcagta
    14521 cataatattg cgccgtcagt tgggcgacgt taatcaaatc actctgttgt gcaggttggc
    14581 ctgcacgatt gtggattgcc attgctttgt cctttgtctg caacgtttta gatagttccg
    14641 caaaccttct caatcaattc cgccgggaat tgcatcgact gcatgatgtg ttcgatcatg
    14701 ctgcatttac ggccggtgtt ggtgttggtg atcacccaat acggcgtgcc tggcacatgt
    14761 ttcggcttgg tctgattacc atttttcagc agcgtttgtt catctgccgc aaagtacacg
    14821 cgtgtacgac cgtgcaacga ttccgttgct tcggcaaacg cctgggcgtc aagagaatat
    14881 agtgtagaca acagcagcat aaagcgattg accgctcgct tttgctctgc gtattcgtcc
    14941 gaaagcagaa gttcacgcat tgcgcgaacc ttgtctttaa tcgttttgac cggcttcgct
    15001 tcgacgatag caggtgacgc aacgcgaacc tctttcgtca ccggagcagc aggctgtgat
    15061 gcggcggaaa atttcaacat acgccgtaaa atgtcggatg cgctctcgcc gatatgctta
    15121 gtgtggctgg caatatagct gtagagttca tcatcaactt caatcgtttt catcttaatc
    15181 cagtgcagtg tcttagctga atacaagtcg ccaagagtat atgaataaat cccggcagcg
    15241 gatagcgtca agcctggctt gcagaaaaac tcggaataaa ccttatttgt tgcagccctg
    15301 tggcacaatg gtcaacataa taccctaacc cgacaacgcg aaaaaaagaa ctttgccatg
    15361 aaattaaata tccgcgcgca aactgcacaa aaccagcaca ataattctcc catcgttctt
    15421 gtccatggtc tgtttggtag cctcgacaac cttggcgtac tggctcgcga tctggtaaac
    15481 gatcacaata tcatccaggt tgatatgcgt aaccacggtc tttcaccgag agagccggta
    15541 atgaattacc cggcgatggc gcaggatctt gttgataccc tggatgcact gcagatcgac
    15601 aaagcaacat ttatcggtca ctccatgggc ggtaaagcgg taatggcact tactgcactg
    15661 gcccccgatc gcatcgataa actggtggcg atcgatatcg caccggtcga ctatcacgta
    15721 cgccgtcatg atgagatttt cgcagctatc aacgcggtca gtgaatcgga cgcacaaact
    15781 cgccagcaag cagcagcaat aatgcgccag catcttaatg aagaaggggt gattcagttt
    15841 ctgttgaaat cttttgttga cggggagtgg cgctttaacg tgccggtatt gtgggatcag
    15901 tatccgcata ttgtgggttg ggagaaaatc ccggcatggg atcaccctgc cctgtttatc
    15961 cctggcggca attctccgta tgttagcgag cagtaccgtg atgatttact ggcacaattt
    16021 ccacaggcac gggcgcatgt gattgcaggc gcgggtcact gggtccatgc tgaaaaaccg
    16081 gatgcggtat tacgcgctat ccgtcgctat ctcaatgatt aacgcaactg attaaaaacc
    16141 cagcgtccgc gtcgctttcc gcgcgtgggc gttggcgtga ctcatcgcgc tgatgtatta
    16201 tggcgcgcta tccatccggg cagaggcctg gctgattgtt tcccccgaag tcaccaagat
    16261 catggccaaa gaacaaacgg accgtacgac attagatctg ttcgcgcacg agcgtcgacc
    16321 gggacgaccg aaaactaatc cgctttcgcg cgatgaacag ctgcgtatta ataaacgcaa
    16381 ccagctaaaa cgcgacaaag tacgtggcct taagcgtgtc gaactgaagc tgaacgcgga
    16441 agctgtcgag gcgctgaacg agctggcgga atcgcgtaat atgagccgta gcgaactgat
    16501 cgaagagatg ttaatgcagc aactggcggc gctgcgtagt cagggcatcg tttaaatttc
    16561 cactttcatg tagcacagtg tgcagtcctg ctcgtttgct gattccgcaa gactgcctgt
    16621 tctgctatga ttgcctttat ccgtgggcaa ttttccaccc ccatttcaat aagtttcaag
    16681 aggttatttc actcatggct atcactggca tctttttcgg cagcgacacc ggtaataccg
    16741 aaaatatcgc aaaaatgatt caaaaacagc ttggtaaaga cgttgccgat gtccatgaca
    16801 ttgcaaaaag cagcaaagaa gatctggaag cttacgacat tctgctgctg ggcatcccaa
    16861 cctggtatta cggcgaagcg cagtgtgact gggatgactt cttcccgact ctcgaagaga
    16921 ttgatttcaa tggtaaactg gttgcgctgt ttggttgtgg tgaccaggaa gattacgccg
    16981 aatatttctg cgacgcattg ggcaccatcc gcgacatcat tgaaccgcgc ggtgcaacca
    17041 tcgttggtca ctggccaact gcgggctatc atttcgaagc atcaaaaggt ctggcagatg
    17101 acgaccactt tgtcggtctg gctatcgacg aagaccgtca gccggaactg accgctgaac
    17161 gtgtagaaaa atgggttaaa cagatttctg aagagttgca tctcgacgaa attcttaatg
    17221 cctgatgtga tgcggcgtag actcatgtct acgccgtatt aatagataat gccaatcaaa
    17281 ataattgcta caaatttgta acttttgctg ttgtacctgt acaatgtccc agtgttcaag
    17341 tggccttgcc gttgtaaatg taagctgtgc cacgttttta ttaacaatat ttgccaggga
    17401 cttgtggttt tcatttaggc gtggcaattc tataatgata cgcattatct caagagcaaa
    17461 ttctgtcact tcttctaatg aagtgaaccg cttagtaaca ggacagattc cgcatgactg
    17521 ataacaatac cgccctaaag aaagctggcc tgaaagtaac gcttcctcgt ttaaaaatct
    17581 tggaagttct tcaggagccg gacaaccatc acgtcagtgc ggaagattta tacaaacgtc
    17641 tgatcgatat gggtgaagaa attggtctgg ctacggtata tcgcgtactg aaccagtttg
    17701 acgacgctgg tatcgtcacc cgccacaatt ttgaaggcgg taaatccgta tttgaactga
    17761 cacagcaaca tcaccacgat cacctgattt gcctcgactg cggcaaggtt atcgaattta
    17821 gtgatgattc catcgaagcg cgtcagcgtg aaattgccgc aaaacatggc attcgcctga
    17881 ctaaccacag tctctatctt tacggtcact gtgccgaagg cgattgccgc gaagatgagc
    17941 acgcgcacga aggcaaataa gccagcctga acgagaaaag ccaacctgcg ggttggcttt
    18001 tttatacaag ggaaataaga aaagcgagag ttacagctct cacttatttg ttattactgc
    18061 gtatttcctg ccagacctta tcacaatcgg ctttcaccgc ctgatcattt ccggtttgcg
    18121 ttgcacgcag acactgctga taatccagta cgcgtacact ttcctggtcg gcaaactgct
    18181 gatgttgttt ctctttcttc agcacgttta acacgctctg gcaggcttca attttttctg
    18241 gcgaaccttg cgcggtgttg atacaggcgc tgtatgcctc tttcagacgg ctgtcttcct
    18301 gtggtgcggt tgattgcgca caagctacca gccccgatgc catcatggcg atgagaatca
    18361 gttttttcat tgaatgcggc aggcacaaaa cctgccgcgt cgggcatcag aagatggtga
    18421 atggtgcgat taccataaat ttcacgtcac gctcatcctg gaagatgtta ccgtaaccgc
    18481 cgccccagct tgggatgtcg gagtggttgt cgtattcggt gaagtgcagt ttgaacatcg
    18541 tgcctttggc gcgaccgtcc tgaatggtgt agaccgcatc caggctgtag gcagactctt
    18601 caatagtccg gtttttgtcg tagtacgcat ccggattgct ctgccaggtc gcaggtttag
    18661 catcccatgc gtaaacgtag gaagcgccga tggcgaagcc tggaagattc cagtttttca
    18721 ggtcatacat cgcactgaag aagaccgctt tttcgccgtt agcgttgaag tcggaacggt
    18781 tatcccacca gatatccagg cggccgttgg aggaagcgta ggttggggtc atacgttgca
    18841 ggaagtatcc ctgctgactg tcagccttaa tccaggtgcc ttcgaggcgc aaatccacta
    18901 cgtcagccgc ccggtaacca aaggtcaacg cttgcagcca ggcggtgccg tcataaagat
    18961 cgttgacgct gcgatcgtca actttatcgc gggtaccata gaactggtag ctggtggtta
    19021 acgggctacc ggcgatatca aatttgtagc tggctttggc aaaatattga tcgatatacc
    19081 cttccgcctg accaaatgcc gcttccagta cgaagttatt tttgaagtcg tatttcgcac
    19141 cgatggagtg cagataatca actttggtgg ttttatcgtt ctgataaaac tcatccattt
    19201 ccagatgcca cggcgctttg tattcgttgg tccacatgta ggagaaactc aaggcaccag
    19261 catcgccgta atcaaaattc gccccggctt ccgccccctg ataagtaccc ggcataaagc
    19321 tccagtgcgg cgctaacagc gtttgaccgg ttggctgaat gtaacctgcc ctcgcccaaa
    19381 ccggaccgta tttaaatttg gccgcagctt tatacaggct tataccgctt ttgtcgccgg
    19441 accagtcttc gtcataggct ttattacttt ttgaaaacgc gatttcgttc gggtgggagc
    19501 tgtcgccgtt ttccgccatt tcaatcgccg taaacgcagc aatatcaagg ccgaacatat
    19561 cagcagcata accggactga aaatcgaggt tggcattcca ggtggagtga gaaaggttgg
    19621 ttttgtattt gtcgccgtcg gtaacatctt tacggtcgcg ttcacgctgc cagtaataga
    19681 taccgccggt taaggttgaa tcgtcgatga atccttcggc gcgagcctcc ggcattgcca
    19741 taaagcccga cattgctgta acaccggcaa tagccagcgc cagcgtacta cgtttgccac
    19801 taaacgtacg catgggttaa tcctctttga cgtataaatt gctgcaccaa aggtgaacag
    19861 cgaaaaaaaa taaaaattaa aaaagtagcg ttgagaaaag tctcgctgac tacaggaaca
    19921 gactattttt cgcgacgcga attatcaatc tctttctgta accagaatgt aagaacatga
    19981 gcaaccgcac atattttgat gatttttgtt acaaggctta tgtttgcggt tgaaaggctt
    20041 gcagaagaat aaaaacgtga aattccagca agatgaaagc ggttacattt tatttaatta
    20101 tgttttgtga ttttaaattt attttatgga taaattgcga agcggaatta attcgcgaag
    20161 aaaagcagaa aaaacgccgc agagcgcggc gtttgggatt gtttgctatt tcttgccgga
    20221 tgcggcgtga acgccttatc cggcctacaa aagcacgcaa attcaatata ttgcagagat
    20281 catgtaggcc tgataagcga agcgcatcag gcaatttagc gtttgaaatt tattgtctat
    20341 ttattgccgg atgcgacgta aatgcctcac ccggcttggt agaacttaaa attactcgcc
    20401 tactttcgcc caggtatcac gcagcccaac ggtgcggtta aataccggtt tttcggccgt
    20461 ggaatggcgg ctgtcgaggc agaagtaacc ttcacgctca aactggaatg ctttacccgc
    20521 aaccgcatct ttcagcgacg gttcagcaaa gccctgtttg atcaccagcg attccgggtt
    20581 aatcaccgac aggaaatcat ccgcagcacc tgggttaggc acgctgaaca gacgatcata
    20641 caaacggatt tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa cacctttgac
    20701 tttacgacca tctgccggat ctttgcttaa ggtgtcggcg tcataagtac agaagatggt
    20761 ggtgatatta ccttcggcat ctttctccac gcgttcagcc ttaatgacgt aagcattacg
    20821 cagacgcact tctttaccca gcaccagacg tttgtactgc ttgttagctt cttcgcggaa
    20881 atcggcgcga tcgatccaaa tttcaccgct aaacggcacc tgacggctcc ccatttccgg
    20941 tttgttcgga tggttcggca tggtgaccat ttcgccttcg ccctgatagt tttcgataac
    21001 cagtttcacc ggatcgataa ccgccattgc gcgcggcgca ttttcgttga gatcttcacg
    21061 gatgcaggat tccagcgacg ccatctcaat ggtgttgtcc tgcttggtca cgccgatgcg
    21121 tttgcagaac tcacgaatag aagccgcagt gtaaccacga cgacgcagac cggaaatggt
    21181 cggcatacgc gggtcatccc agccttcaac gtgcttgtcg gtcaccagca ggttcagctt
    21241 acgcttggac atcacagtgt attccagatt caggcgcgag aattcgtact ggcgcggatg
    21301 aacaggaatg gtgatgttat ccagtaccca gtcgtacaga cgacggttgt cctggaactc
    21361 aagcgtacac agagagtgcg taataccttc cagcgcatcg ctgatgcaat gggtgaagtc
    21421 gtacatcggg tagatgcacc acttgttgcc agtctggtgg tgttcagcaa atttaatgcg
    21481 gtacagcacc ggatcgcgca ttacgataaa tggcgacgcc atgtcgattt tcgcacgcag
    21541 gcaggcttta ccttcttcaa aaccaccggc acgcattttt tcgaacagcg ccaagttctc
    21601 ttcaacgctg cggtcgcggt acgggctgtt tttacccggc tgcgtcaggg taccgcggta
    21661 ttcgcggatc tgttccggtg tcagttcatc aacgtacgcc aggcctttat tgatcagttc
    21721 gatcgcatag gcgtggagct gatcaaaata atcggaggag taacggacgt taccagacca
    21781 gtgaaaacct aaccactcta cgtcgttttt gatcgaatcg acatactcga tatcttcttt
    21841 taccgggtta gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct gggcgatccc
    21901 gaagttcagg cagatagatt tcgcatggcc aatatgcaga tagccattcg gctccggcgg
    21961 gaaacgggtg tgtactgtga tgtgcttacc actggccaga tcttcatcga tgatctgacg
    22021 gataaagtta gtcgggcggg cttctgcctc actcatcgtg gattcctcaa agcgtaaaca
    22081 acgtataacg gcgtatgatc ttataagcgg gacaggctga caactgttag ttttttaaaa
    22141 ttgataatct aacaaggatt atggggatga ttgatgaaaa aaaaagcggc agagaagatc
    22201 tccaccgctt gtcattgttg gatgcgacgc tgccgtgtcg catcaggcat aaagcagatt
    22261 actttttgat ttcatacagc ggtgtttgac ccgccacaac atggccctga gctttaatga
    22321 gcaagccact gaaatcgtcg atattgctgc aaaccaccgg gctaatcatc gagcgggcgt
    22381 tagcgttcag gtaatccaga tccatttcca gaatcggttg ccctgcgctt acctgcgcac
    22441 cctcttccac cagacgttta aagcctttac cttccagcgc tacggtgtcg atacccatat
    22501 ggacgacgat ctccgcgcct ttttcggttt ccaggcagaa cgcgtggttg gtgttgaaga
    22561 ttttcacgat tgtccctgcg gctggtgata cgacgatttt atctgtcggt ttcaccgcca
    22621 caccgtcacc caccgctttg ctggcgaatg cttcgtcagg aacctgatcc agtgccacga
    22681 catcaccggt aatcggcgat accagctccg cgatagatac cgcgtttggt acagcctgcg
    22741 gttttgctac aggcgcggca gttgccggag ttgcttcagc tgacgcagcg gctaccggac
    22801 cacgggcaac gactttcttc atcgcatcgc cgatggattc tgctttcgcg ccaacaatca
    22861 cctgaatagt ctgtttgttc agtttcacta caccagaagc acccagacgt ttacacatcg
    22921 tatcgttgac gcgggcagag tcagccacgg tcaggcgcag acgggtaata caggcatcaa
    22981 tcgctttcag gttgtcagta ccgccaaccg cagcaatata gttggttgcc agttgagtca
    23041 gaccttcttc agtattgctg ttggcttctt cagtaacgat ctcgtcttct ttatcttcac
    23101 gacccggcgt tttcaggttg aacatgcgga taaccaaact gaacaccacg aagtagatag
    23161 cgaagaagat aacgcccatc accagcagca tccagacgtt ctggctggcg gcaggcaggt
    23221 tatacatcaa cgcgtagtcg atagcacccg cagagaaaga gaagccggca tggataccca
    23281 gcagcgttgc cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg tacagcagcg
    23341 gcgcaaggaa catgaacagg aattccagcg gctcagtcac accggtcagg aacgcagtaa
    23401 cagcaacaga aagcagcatc ccgccaacca tcggacgacg ctctttcggt gctgcgaagt
    23461 acatcgccag cgccgcaccc ggcagaccga acatcatgat cgggaagaag ccggacatga
    23521 acatccccgc ggtgccgtca ccagcgtaga aacggttgat gtcgccgtgg aaaaccgtac
    23581 ccgccgcgtt ggtgaattca ccaatctgga accaggcgat ggtgttcagc acctgatgca
    23641 gaccggttgg gatcagcaga cggttgatga aaccaaagat accggaaccc agcgcgcccg
    23701 cagaaacgat ccactcgccg cctgcatgga tagcgtgctg taccggcggc cagacgtaac
    23761 caaaaatggc cgccagcacc agacagaaga agccggtggc gatcggcaca aagcgtttgc
    23821 cgccgaagaa gctcaggaag tccggcagtt taatatcgga ccaacggtta taggctgcgc
    23881 caccaaccag accggtaatg atacccgcca gtacacccat gttaatttct gggttgatgg
    23941 tcaccatcgc tttggttaac acaaagtaac ctaccgcacc cgccagcgcc gccgcacctg
    24001 cgctgtcttt cgaccagctg gatgccacac cgatggcgaa gattaatgcg aggttatcaa
    24061 aaattgcacc gcccgcctgg gcaataaacg caacgttaag taaatctggc tgaccgaatc
    24121 gcagcaacag cgccgccacc ggcagcaccg cgatagggag ctgtaacgcc ctaccgagtc
    24181 gctggaaaaa acctaaaata ttcatcttat tccccctacg agaaccctat ttggctcgtt
    24241 taaagccgta tttttatttt gctgcaaatt ttactgccga tgttctgtaa tcagattgtt
    24301 agatcatctg ctacagagtg tgtgaaaatt taattcgcat cgcaaattaa acgcgtgtct
    24361 tttgtgagtt ttgtcaccaa atatcgttat catcactccc ttttactggc taaaccagaa
    24421 aacttatttt atcattcaaa aaatcaagtc ggattgacgc ctgtctgcgc aaatccaggt
    24481 tacgcttaaa gatgtctaat ccgccaacgg cttacatttt acttattgag gtgaataatg
    24541 agactgatcc ccctgactac cgctgaacag gtcggcaaat gggctgctcg ccatatcgtc
    24601 aatcgtatca atgcgttcaa accaactgcc gatcgtccgt ttgtactggg cctgccgact
    24661 ggcggcacgc cgatgaccac ctataaagcg ttagtcgaaa tgcataaagc aggccaggtc
    24721 agctttaagc acgttgtcac cttcaacatg gacgaatatg tcggtctgcc gaaagagcat
    24781 ccggaaagct actacagctt tatgcaccgt aatttcttcg atcacgttga tattccagca
    24841 gaaaacatca accttctcaa cggcaatgcc ccggatatcg atgccgagtg ccgccagtat
    24901 gaagaaaaaa tccgttctta cggaaaaatt catctgttta tgggcggtgt aggtaacgac
    24961 ggtcatattg catttaacga accggcgtct tctctggctt ctcgtactcg tatcaaaacc
    25021 ctgactcatg acactcgcgt cgcgaactct cgtttctttg ataacgatgt caatcaggtg
    25081 ccaaaatatg ccctgactgt cggtgttggt acactgctgg atgccgaaga agtgatgatt
    25141 atggtgctgg gtagccagaa agcactggcg ctgcaggccg ccgttgaagg ttgcgtgaac
    25201 catatgtgga ccatcagctg tctgcaactg catccgaaag cgatcatggt gtgcgatgaa
    25261 ccttccacca tggagctgaa agttaagact ttaagatatt tcaatgaatt agaagcagaa
    25321 aatatcaaag gtctgtaatt gttatccctg ccctggttcc ttgctcaggg caatattttt
    25381 taaaatcggg ggtcagaatg tatgcattaa cccagggccg gatctttacc ggccacgaat
    25441 ttcttgatga ccacgcggtt gttatcgctg atggcctgat taaaagcgtc tgtccggtag
    25501 cggaactgcc gccagagatc gaacaacgtt cactgaacgg ggccattctc tcccccggtt
    25561 ttatcgatgt gcagttaaac ggctgcggcg gcgtacagtt taacgacacc gctgaagcgg
    25621 tcagcgtgga aacgctggaa atcatgcaga aagccaatga gaaatcaggc tgtactaact
    25681 atctgccgac gcttatcacc accagcgatg agctgatgaa acagggcgtg cgcgttatgc
    25741 gtgagtacct ggcaaaacat ccgaatcagg cgttaggtct gcatctggaa ggtccgtggc
    25801 tgaatctggt aaaaaaaggc acccataatc cgaattttgt gcgtaagcct gatgccgcgc
    25861 tggtcgattt cctgtgtgaa aacgccgacg tcattaccaa agtgaccctg gcaccggaaa
    25921 tggttcctgc ggaagtcatc agcaaactgg caaatgccgg gattgtggtt tctgccggtc
    25981 actccaacgc gacgttgaaa gaagcaaaag ccggtttccg cgcggggatt acctttgcca
    26041 cccatctgta caacgcgatg ccgtatatta ccggtcgtga accgggcctg gcgggcgcga
    26101 tcctcgacga agctgacatt tattgcggta ttatcgctga tggcctgcat gttgattacg
    26161 ccaacattcg taacgctaaa cgccttaaag gcgacaaact gtgtctggtt accgacgcca
    26221 ccgcgccagc aggtgccaac attgaacagt tcatttttgc gggtaaaaca atatactacc
    26281 gtaacggact ttgtgtggat gagaacggta cgttaagcgg ttcatcctta accatgattg
    26341 aaggcgtgcg taatctggtc gaacattgcg gtatcgcact ggatgaagtg ctgcgtatgg
    26401 cgacgctcta tccggcgcgt gcgattgacg ttgaaaaacg tctcggcacg ctcgccgcag
    26461 gtaaagtagc caacctgacc gcattcacac ctgattttaa aatcaccaag accatcgtta
    26521 acggtaacga ggtcgtaact caataagaga aagtatgaca ccaggcggac aagctcagat
    26581 aggtaatgtt gatctcgtaa aacagcttaa cagcgcggcg gtttatcgcc tgattgacca
    26641 gtacgggcca atctcgcgga ttcagattgc cgagcaaagc cagcttgccc ccgccagcgt
    26701 aaccaaaatt acgcgtcagc ttatcgaacg cgggctgatc aaagaagttg atcagcaggc
    26761 ctccaccggg ggccgccgcg ctatctccat cgtcaccgaa acccgcaatt tccacgcaat
    26821 cggcgtacgg cttggtcgtc atgacgccac catcactctg tttgatctca gcagcaaagt
    26881 gctggcagaa gaacattacc cgctgccgga acgtacccag cagacgctgg aacatgccct
    26941 gctgaatgcc attgctcagt ttattgatag ctaccagcgc aagctgcgcg agctaatcgc
    27001 catttcggtg atcctgccag ggcttgttga cccggacagc ggcaaaattc attacatgcc
    27061 gcatattcag gtagaaaact gggggctggt agaagcactg gaagaacgtt ttaaagtgac
    27121 ctgtttcgtt ggtcacgata tccgtagtct ggcgctggcg gagcactact tcggtgcaag
    27181 tcaggattgc gaagactcca ttctggtgcg tgtccatcgc ggaaccgggg ccgggattat
    27241 ctctaacggg cgcattttta ttggccgcaa cggtaacgtc ggtgaaattg gccatattca
    27301 ggtcgaaccg ctgggtgaac gctgccactg cggcaacttt ggctgcctgg aaactatcgc
    27361 cgccaacgct gccattgaac aacgggtgtt gaatctgtta aagcagggct accagagccg
    27421 tgtgccactg gacgactgca ccatcaaaac tatctgcaaa gccgcgaaca aaggcgatag
    27481 cctggcctcg gaagtgattg agtatgtcgg tcgtcatctg ggtaaaacca tcgccattgc
    27541 tatcaactta tttaatccgc aaaaaattgt tattgctggt gaaatcaccg aagccgataa
    27601 agtgctgctc cctgctattg aaagctgcat taatacccaa gcgctgaagg cgtttcgtac
    27661 taatctgccg gtggtacgtt ctgagctgga tcaccgctcg gcaatcggcg cttttgcgct
    27721 ggtaaaacgc gccatgctca acggtatttt gctccagcat ttgctggaaa attaatgtgc
    27781 ttttatagtg gcgcttattg ttgtcaatat tctgggtagt ccatgaccat taaaaatgta
    27841 atttgcgata tcgacggcgt gctgatgcac gataacgtcg ccgtaccggg tgcagcggaa
    27901 tttttgcacg ggattatgga taaaggcctg ccgctggtgt tgctgaccaa ctatccttcg
    27961 cagactgggc aagatctggc gaaccgcttt gccactgccg gtgtcgatgt gccggatagc
    28021 gtgttttata cctctgcgat ggcgaccgcc gatttcctgc gtcgccagga aggcaagaaa
    28081 gcgtatgtgg tgggcgaagg cgcactgatt catgaactgt acaaagccgg tttcactatt
    28141 accgatgtga accctgattt cgtgattgtt ggcgaaacac gttcttacaa ctgggacatg
    28201 atgcataaag cagcctattt cgtcgctaac ggtgcacgct ttatcgccac caacccggac
    28261 acccacgggc gcggttttta tcccgcttgt ggcgcgttgt gtgcagggat tgagaaaatc
    28321 tccgggcgca aaccgttcta tgttggtaag cccagcccgt ggatcatccg cgcggcatta
    28381 aacaaaatgc aggcgcattc ggaagaaaca gtgattgtcg gcgataacct gcgtaccgat
    28441 attctggcgg gcttccaggc cggtctggag acgattctgg tgctttctgg tgtttcatcg
    28501 ctcgacgata tcgacagtat gccgttccgc ccaagctgga tttacccgtc agtcgctgaa
    28561 atcgacgtta tctgaatacc acccacgtct gcggacgtgg ttggctctga tgacaaacgc
    28621 caaactgcct gatgcgctac gcttatcagg cctacgcagc tcctgcaata tattgaattt
    28681 tcatgctttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac aaatcgcact
    28741 ttatcagcaa tttggccccc gtaaaatata accattcaat aaaacccgct aaaaattatc
    28801 gattcatcaa taaatacatc ctcacaccag accctttttc accaatcgac aatcaccatt
    28861 acgtttttta tttttgcact gccaaatagc ttgcgcacag tgctgctttg cggcattttt
    28921 ttaaacaagc aaacacaaca agcaacaaat accaggttaa cggagaaggt tatgtgttca
    28981 atttttggcg tattcgatat caaaacagac gcagttgagc tgcgtaagaa agcactcgag
    29041 ctgtcacgcc tgatgcgtca tcgtggcccg gactggtccg gtatttatgc cagcgataac
    29101 gccattctcg ctcacgaacg tctgtcaatt gttgacgtta acgcgggggc gcaacctctc
    29161 tacaaccaac aaaaaaacca cgtactggcg gtaaacggtg aaatctacaa ccactaggca
    29221 ttgcgcgctg aatatggcga tcgttatcag ttccagacag ggtctgactg tgaagtgatc
    29281 ctcgcgctgt atcaggaaaa agggccggaa tttctcgacg acttgcaggg catgtttgcc
    29341 tttgccctgt acgacagcga aaaagatgcc tacctgattg gtcgcgacca tctggggatc
    29401 atcccactgt atatggggta tgacgaacac ggtcagctgt atgtggcctc agaaatgaaa
    29461 tcgctggtgc cagtttgccg cacgattaaa gagttcccgg cggggagcta tttgtggagt
    29521 caggacggcg aaatccgttc ttattatcat cgcgactggt tcgactttga tgcagtgaaa
    29581 gataacgtga ccgacaaaaa cgagctgcgt caggcactgg aagattccgt taaaagccat
    29641 ctgatgtctg atgtgcctta cggtgtgcta ctttctggtg gtctggattc ctcaattatt
    29701 tccgctatca ccaagaaata cgcagcccgt cgcgtggaag atcaggaacg ctctgaagcc
    29761 tggtggccgc agttacactc ctttgctgta ggtctgccgg gttcaccgga tcttaaagca
    29821 gcccaggaag tggcaaacca tctgggcacg gtgcatcacg aaattcactt cactgtacag
    29881 gaaggtctgg atgccatccg cgacgtgatt taccacatcg aaacttatga tgtgaccact
    29941 attcgcgctt caacaccgat gtatttaatg tcgcgtaaga tcaaggcgat gggcattaaa
    30001 atggtgctgt ccggtgaagg ttctgatgaa gtgtttggcg gttatcttta cttccataaa
    30061 gcgcccaacg ctaaagaact gcatgaagag acggtgcgta aactgctggc cttgcatatg
    30121 tatgactgcg cgcgcgccaa caaagcgatg tcagcctggg gcgtggaagc acgcgttccg
    30181 ttcctcgaca aaaaattcct tgatgtggcg atgcgcatta acccgcagga taaaatgtgc
    30241 ggtaacggca aaatggaaaa acacatcctg cgtgaatgtt ttgagtcata cctgcccgca
    30301 agcgtggcct ggcggcagaa agagcagttc tccgatggcg tcggttacag ttggatcgac
    30361 accctgaaag aagtggcggc gcagcaggtt tctgatcagc aactggaaac tgcccgcttc
    30421 cgcttcccgt acaacacgcc gacctcaaaa gaagcgtatc tgtaccgcga gatctttgaa
    30481 gaactgttcc cacttccgag tgccgctgag tgcgtgccgg gcggtccttc cgtcgcgtgt
    30541 tcttcagcta aagcgatcga atgggatgaa gcgttcaaaa aaatggacga tccgtctggt
    30601 cgtgcggttg gcgttcacca gtcggcgtat aagtaagaaa tgcggtacag ccccggagaa
    30661 atccggggct gttttagtta ttgcgctgct tgttgattct gatatgcagt aaggaaatgt
    30721 tccgggtttg ccacatcact gtttgacaac tcatcactgt aggccagtat ttcatagatt
    30781 aatttcccac cactattgtt gaattccgaa tttcatcctg tacgacaaaa gaatggctgt
    30841 agggtgggga aaaacacaaa gtaaacaata attgacgaat atagcgccac gctgttcgca
    30901 acctaaccaa acagtcactt tcgagcaatt ttccttgaaa aagaggttga cgctgcaagg
    30961 ctctatacgc ataatgcgcc ccgcaacgcc gataaggtat cgcgaaaaaa agatggctac
    31021 gtagctcagt tggttagagc acatcactca taatgatggg gtcacaggtt cgaatcccgt
    31081 cgtagccacc atcttttttg cgggagtggc gaaattggta gacgcaccag atttaggttc
    31141 tggcgccgca aggtgtgcga gttcaagtct cgcctcccgc accattcacc agaaagcgtt
    31201 gtacggatgg ggtatcgcca agcggtaagg caccggtttt tgataccggc attccctggt
    31261 tcgaatccag gtaccccagc catcttcttc gagtaagcgg ttcaccgccc ggttattggg
    31321 gtatcgccaa gcggtaaggc accggttttt gataccggca ttccctggtt cgaatccagg
    31381 taccccagcc atcgaagaaa taatctggct acgtagctca gttggttaga gcacatcact
    31441 cataatgatg gggtcacagg ttcgaatccc gtcgtagcca ccaaattctg aatgtgtcga
    31501 atacgttcgg caaattcaaa aaccaatttg ttggggtatc gccaagcggt aaggcaccgg
    31561 attctgattc cggcattccg aggttcgaat cctcgtaccc cagccaattt attcaagacg
    31621 cttacatcgt aagtgcaccc agttggggta tcgccaagcg gtaaggcacc ggattctgat
    31681 tccggcattc cgaggttcga atcctcgtac cccagccaca ttaaaaaagc tcgcttcggc
    31741 gagctttttg cttttctgcg ttcattcaat gtcgaatgcg atgttgacac gtcttatcct
    31801 tcaatgtcgg atgcgacgct gccgcgtctt atccgaccta cggctggcac gcatccggca
    31861 atgttgtaag gctacaaccc taacgcatat ttcagcgcct gacgtttcaa cacgccagca
    31921 cgctctgccg ccattaaccc gagattacgc acaaaacgca gcggcggcag attattactg
    31981 aatccggcat aaaacagatc cataccactt tgcataatga agttatccgc catgcgccgc
    32041 atctggtaac gcttgaggac aggataactg gcccacgctt caccgtagct gcgggcattc
    32101 accagaacat caatcagggc atcgacatca cgataaccaa gattcacccc ctgccccgcc
    32161 agcggatgga tggtatgcgc ggcatcgccc accagcgcaa gccccggctg cacatactgc
    32221 aacgcatgtc ggcgcgtcag cggaaacgca ccagcggcaa gcggtgtaac gtaacccaga
    32281 cgcgacggga aatgcttcgc gatttccgcc tggagttgcg ccatattcat attctgcaac
    32341 tggcgaatac gcgccggaga gtcataccac accagcgatg cccagttatc aaacaacggc
    32401 agaaacgcac gcggtccgtc cggggtaaat tgctgccagg tgctgtcgcc gggatcgttc
    32461 tcacactgta cgctaatcaa catgcacgac tgcgcatact gccaggcatg aacgccaatt
    32521 cccgccatct gccgcacctg agaatttgcg ccgtcggcac caatcaccag tttcgcgcgg
    32581 atcgtttcac cgcccttcag ttccagctcc tgaagatcat tatctcgatg taatgcaatc
    32641 agcgaggttg gcacacgtaa cgttactttc ggatgcgctt ccagcgcctg ccacaacgcc
    32701 tgttgcagga ctgtgttttc caccatgtag ccaagcaacg gcaactttaa ttcggctgcg
    32761 tcaaatacca catgtgccgt ttcccactcc caggtttcca gtctgcggta aggatggcaa
    32821 cgcatagcct gtactgcatc ccagacccct aaccctttaa gcaatgatac cgaagccgcg
    32881 ctgatcgccg agatccgcac gtccggttgg ctgtcagcga caaacggtgc aggttctgcg
    32941 tgctcgatca ccgttaccga aaatccgtgc tgtgccagcc ccagcgccag tgcgccgccg
    33001 accattcctc cgccgacaat ggcaatttcc gttggttgat ttgtcatggt caatcatcct
    33061 gttagcaata tgctttaagt ttacaggatt tttaccccca agggctgata acgctcatac
    33121 tggtcacaac ggcagcaaag cattacacta tgcgccctgc attcctggct actatttcgc
    33181 aagagcaagt cgatgaccaa aaaactccat attaaaacct ggggctgtca gatgaacgag
    33241 tacgattcat cgaagatggc cgatctgctg gatgccaccc acggctatca actgaccgac
    33301 gtggcggaag aagcggatgt gctactgctg aacacctgct caatccgcga gaaggctcag
    33361 gaaaaagtct tccatcagtt gggtcgctgg aaactgttaa aagagaagaa tccagacctg
    33421 attatcggcg tcggtggctg cgtggcatcg caagaaggcg agcacattcg ccagcgcgcc
    33481 cactatgtcg atattatttt tgggccgcaa acgctgcacc gtctgccgga gatgatcaac
    33541 tccgtgcgcg gcgaccgcag cccggttgta gatatcagct tcccggaaat cgagaagttt
    33601 gaccgtctgc cggaaccgcg cgccgaaggg ccgaccgcgt ttgtctccat catggaaggc
    33661 tgcaataaat attgcaccta ctgcgtggtg ccttacaccc gtggtgaaga ggtaagccgt
    33721 ccgtctgacg atattctgtt tgagattgcc cagcttgcgg ctcagggcgt gcgtgaagtc
    33781 aacctgctcg gtcagaatgt gaatgcctgg cgtggtgaga actacgacgg caccaccgga
    33841 tcgtttgccg atctgttgcg tctggttgct gcgatcgacg ggatcgatcg tattcgcttt
    33901 accaccagcc atccgatcga attcaccgac gatatcatcg aagtgtaccg cgacacgcct
    33961 gagctggtga gcttcctgca tctgccggta cagagcggtt ccgatcgcat tctgaacctg
    34021 atgggacgta cccatacggc gctggagtac aaagcgatca tccgtaaact gcgtgcggcg
    34081 cgtccggata ttcagatcag ttctgacttc attgttggct tccctggcga aaccaccgaa
    34141 gacttcgaga aaacgatgaa gctgattgcc gacgtcaatt tcgacatgag ctacagcttt
    34201 atcttctctg cacgtccagg aacgcctgcc gccgatatgg ttgatgatgt tccggaagaa
    34261 gagaagaagc agcgtctgta tattctgcaa gaacgtatca atcagcaagc gatggcatgg
    34321 agccgtcgga tgctcggcac cacccagcgt attctggtag aagggacttc gcgtaagagc
    34381 atcatggagc tttccgggcg taccgaaaat aaccgcgtgg tcaacttcga gggcacaccg
    34441 gatatgatcg gtaaattcgt cgatgtagaa attaccgacg tctacccgaa ctctctgcgc
    34501 ggtaaagtgg tgcgaactga agatgaaatg ggtctgcgca tggcagaaac accggagtcg
    34561 gtgattgccc gcacccgcaa agaaaacgac cttggcgtgg gttattatca gccgtaattc
    34621 tcaggcctgc cgttccggtg ggccttgttt tttctctctt tatccccatc tttatcgcaa
    34681 tgcttgcgcc tttttcctgt ggcgtgaata atttccttat aggccggtga atgttcagct
    34741 tccggcggca tacagataag aggaacggtt tgaacataga cactcgcgaa atcaccctgg
    34801 agccagcaga caatgcgcgt ctgttgagcc tgtgcggccc gtttgatgac aacatcaagc
    34861 agctcgaacg ccgtctcggc atcgagatca atcgccgcga taaccacttt aaactgaccg
    34921 gccgtccgat ttgcgtcacc gctgcggcag acattctgcg tagcctgtat gtcgatactg
    34981 ccccgatgcg cggtcagatt caggatatcg aaccggaaca gatccacctt gcgattaaag
    35041 aagcgcgggt actggagcaa agcgcggaga gcgtgccgga gtacggcaaa gcggtcaata
    35101 tcaaaactaa acgcggcgta attaagccgc gcacgccaaa ccaggcgcag tacatcgcca
    35161 atattctcga ccatgacatt accttcggcg ttggcccggc gggtacgggt aaaacctacc
    35221 tggcagtggc tgcggcagtt gatgccctgg agcgtcagga aattcgccgt attctgctga
    35281 ctcgtccggc ggtcgaagcc ggtgagaaac tgggcttcct gcctggcgat ttaagccaga
    35341 aagtagaccc gtatctgcgc ccactgtacg acgcgctgtt tgaaatgctg ggctttgaga
    35401 aagtcgagaa actgattgag cgcaacgtta ttgaagtcgc gccgctggcc tatatgcgtg
    35461 gtcgtacgct gaacgacgcg tttatcattc tcgatgagag ccagaacact accatcgaac
    35521 agatgaagat gttcctgacc cgtatcggtt ttaactcaaa agcggttatc accggcgacg
    35581 tcacacagat cgacctgccg cgtaatacta aatcaggctt acgtcatgcc attgaagtgc
    35641 tggccgatgt cgaagagatc agctttaact tcttccacag cgaagacgtg gttcgtcacc
    35701 cggtggtggc gcgtatcgtt aacgcttatg aagcctggga agaagcagaa caaaaacgta
    35761 aagcggcgct ggcagcagaa cgcaagcgcg aagaacagga acaaaaatga gtcaggtgat
    35821 cctcgattta caactggcat gtgaagataa ttccgggtta ccggaagaga gccagtttca
    35881 aacatggctg aatgcggtga tcccgcagtt tcaggaagag tcggaagtga ctattcgcgt
    35941 ggtcgatacc gccgaaagcc acagtctgaa tctgacctat cgcggtaagg ataagccgac
    36001 caacgtgctc tccttcccgt ttgaagtgcc gcctggcatg gagatgtcgc tactgggcga
    36061 tctggttatc tgccgtcagg tggttgagaa ggaagctcag gagcaaggca aaccactgga
    36121 ggcgcactgg gcgcatatgg tggtgcacgg cagtctgcat ttgttaggtt acgatcacat
    36181 tgaagatgac gaagcagaag aaatggaagc cctcgaaaca gagattatgc ttgctctggg
    36241 ctatgaggat ccgtacattg ccgagaaaga ataatttgcc aggcatttga ctggcagacg
    36301 tccccgccgt tggtgattaa cgacggcgtt ttgattaact aacatgagaa cccatacgac
    36361 gccatgagcg acgacaattc acacagtagt gacacgataa gcaacaagaa gggatttttc
    36421 tccctgttac tcagccaact tttccacggt gaaccgaaaa accgtgacga actgctggcg
    36481 ctgatccgtg attccgggca gaacgacctt atcgacgaag atacgcgcga tatgctcgaa
    36541 ggggtgatgg acatcgcaga ccaacgcgtt cgcgacatca tgatcccccg ctcccagatg
    36601 attaccctga aacgcaacca gacgctggac gaatgccttg atgtcatcat cgagtccgcc
    36661 cactcacgtt tcccggtgat tagcgaagac aaagatcaca ttgaagggat tctgatggcg
    36721 aaagacttgc tgccgtttat gcgcagcgat gctgaagcct tcagcatgga caaagtgtta
    36781 cgtcaggcgg ttgtcgttcc tgaaagtaag cgcgtagacc ggatgctgaa agagtttcgc
    36841 tctcagcgtt accacatggc gatcgttatt gacgaattcg gtggggtttc cggtctggtg
    36901 accattgaag acatcctgga actgattgtt ggtgagattg aagacgagta tgacgaagaa
    36961 gatgatatcg acttccgtca gctgagtcgt catacctgga ccgtgcgcgc actggcttcc
    37021 attgaagact tcaacgaagc gttcggcacc cactttagcg atgaagaagt cgacactatc
    37081 ggtggtctgg tgatgcaggc gtttgggcat cttccggcgc gtggcgaaac tatcgacatc
    37141 gacggttacc agttcaaagt ggcgatggcc gatagtcggc gtattattca ggttcatgtc
    37201 aaaatcccgg atgactcacc ccagccaaag ctggatgaat aaaaccgaaa ctggatagat
    37261 aactacatgg cttttgcctc attaattgaa cgccagcgca ttcgcctgct gctggcgtta
    37321 ttattcggtg cctgcggaac gctggccttc tctccttacg acgtctggcc tgcggcgatt
    37381 atttcgctga tggggcttca ggcgttgacc tttaaccgtc gcccactcca gtctgccgct
    37441 attggctttt gctggggatt tggcctcttt ggcagcggta ttaactgggt ctatgtcagc
    37501 atcgcgacct ttggcgggat gcctggcccg gttaacatct tcctggtggt gctgctggcg
    37561 gcgtatttgt cgctgtatac cggactgttt gctggcgtgc tgtcgcgtct gtggccgaaa
    37621 accacctggc tgcgcgtggc gattgccacc cctgctctct ggcaagtgac cgagtttctg
    37681 cgcggttggg tgctgactgg cttcccgtgg ttacagttcg gctatagcca gattgatggc
    37741 ccgttaaaag ggctggcacc gctaatgggc gtggaagcca ttaacttcct gctgatgatg
    37801 gttagtggcc tgctggcact ggcgttggtc aaacgcaact ggcgtccgct ggtggtggcc
    37861 gtcgtgctgt ttgccctacc cttcccgctg cgttacatcc aatggtttac cccgcaaccg
    37921 gagaaaacca ttcaggtttc gatggttcag ggcgatattc cgcaatcgct gaaatgggac
    37981 gaaggccagc ttcttaatac gctaaagatt tactacaacg caacggcacc gctgatgggc
    38041 aaatcatcgt tgattatctg gccggagtcg gcgataaccg atctggaaat taatcagcaa
    38101 ccgttcctca aagcactgga cggtgagttg cgtgataaag gtagctcgct ggtaaccggg
    38161 attgtcgacg cgcgtctcaa taagcagaac cgctacgata cctacaacac catcatcacg
    38221 ctgggtaaag gtgcaccgta cagctacgaa tcagccgatc gctataacaa aaaccatctg
    38281 gtgccgtttg gcgagtttgt cccgctggag tcgattctgc gtccgttagc accgttcttt
    38341 gatctgccga tgtcgtcgtt cagccgtggg ccatatatcc agccgccgct gtcggcaaat
    38401 ggtattgagc ttactgcggc tatttgctac gagatcattc tcgccgagca agtgcgcgat
    38461 aacttccgcc cggataccga ctacctgctg actatctcca acgatgcgtg gtttggtaaa
    38521 tctattggtc catggcaaca cttccagatg gcgcgaatgc gtgcgctgga gctggcgcgc
    38581 ccactgttgc gcagcaccaa caacggcatt acggcggtga ttggcccgca gggtgagatt
    38641 caggcgatga tcccgcagtt cgcccgcgag gtgttaacca ctaacgtgac gccgaccacc
    38701 ggactcacac catacgcacg taccggcaac tggccgctgt gggtgctgac ggcactgttt
    38761 ggttttgctg ctgtgttgat gagtctgcgt cagcgacgta aataatccct tcttatcgtg
    38821 ccggaataca tcccatattt cggcacgctc accggatgcg gcatcagtgc attatccgga
    38881 taacaatatt ctcctatcca actttggcac atctattgct ttgttataca aggcaaagct
    38941 tgaaccagca tcagtacaac ccggttgcac cacagcaatc caccggtagc accaaaagag
    39001 tgcattttaa gaattttgct tcagaatggt gcggtgcacc tcgcaaaaac aaacaataaa
    39061 acatcaatat ctttacattt agccaaacta aatgttacct aacaggcaca acactgcaca
    39121 ataaagttgc agacgataac aacacaaaca ctcacaacgg gtatccatgc gttcttaacg
    39181 cagaagataa aggtgttgga tatgcaatta cgtaaacctg ccacagcaat cctcgccctg
    39241 gcgctttccg caggactggc acaggcagat gacgccgccc cggcagcagg cagtacgctg
    39301 gacaaaatcg ccaaaaacgg tgtgattgtc gtcggtcacc gtgaatcttc agtacctttc
    39361 tcttattacg acaatcagca aaaagtggtg ggttactcgc aggattactc caacgccatt
    39421 gttgaagcag tgaaaaagaa actcaacaaa ccggacttgc aggtaaaact gattccgatt
    39481 acctcacaaa accgtattcc actgctgcaa aacggcactt tcgattttga atgtggttct
    39541 accaccaaca acgtcgaacg ccaaaaacag gcggctttct ctgacactat tttcgtggtc
    39601 ggtacgcgcc tgttgaccaa aaagggcggc gatatcaaag attttgccga cctgaaaggc
    39661 aaagccgtag tcgtcacttc cggcactacc tctgaagttt tgctcaacaa actgaatgaa
    39721 gagcaaaaaa tgaatatgcg catcatcagc gccaaagatc acggtgactc tttccgcacc
    39781 ctggaaagcg gtcgtgccgt tgcctttatg atggatgacg ctctgctggc cggtgaacgt
    39841 gcgaaagcga agaaaccaga caactgggat atcgtcggca agccgcagtc tcaggaagcc
    39901 tacggttgca tgttgcgtaa agacgatccg cagttcaaaa agctgatgga tgacactatc
    39961 gctcaggtgc agacctccgg tgaagcggaa aaatggtttg ataaatggtt caaaaatcca
    40021 attccgccga aaaacctgaa catgaatttc gaactgtcag acgaaatgaa agcactgttc
    40081 aaagaaccga atgacaaggc actgaactaa ttacaagaac caggggcgga aattccagcc
    40141 ctctcgattg ttacgtagca cggacagact atacgcctga tggtcgttcc ccatcgggcc
    40201 tgaaaaccgc aatacgctgg gtaacaatct tcgagggtag cagttaacgc tgctaccctc
    40261 ttttttctgg agtagattta tgtctataga ctggaactgg ggtatttttt tacaacaagc
    40321 cccgttcggc aataccacct atcttggttg ggtctggagt ggttttcagg tgacgatcgc
    40381 tttgtcgatc tgcgcctgga ttatcgcttt cctcgtcggt tcatttttcg gcattttacg
    40441 taccgttcca aaccgttttc tctccggtct gggtacgttg tatgtcgaac tgttccgcaa
    40501 cgtgccgttg attgtgcaat tctttacctg gtatctggtg atcccggagc tgctgccgga
    40561 gaaaatcggc atgtggttta aggccgagct ggatcccaat attcagtttt tcctttcatc
    40621 catgctctgc ctggggctgt ttaccgccgc ccgtgtttgc gaacaggtac gcgccgctat
    40681 tcagtcgcta ccgcgtgggc agaagaatgc cgcgctggcg atgggcctga cgctaccgca
    40741 gacttatcga tacgtactgc tgcctaatgc ttatcgcgtt atcgtcccgc cgatgacttc
    40801 cgagatgatg aacctggtga aaaactccgc catcgcctcg accatcggtc tggtggatat
    40861 ggcggcgcag gcgggtaaat tgctcgacta ctcggctcac gcctgggagt cgtttaccgc
    40921 catcacgctt gcgtatgtgt tgattaacgc ttttatcatg ctggtgatga cgctggttga
    40981 acgtaaagtt cgcctgcccg gcaacatggg gggcaaataa tgtacgagtt tgactggagt
    41041 tccattgttc cttccctgcc atatctgctc gacgggctgg tgatcaccct gaaaatcacc
    41101 gtcacggcag tcgtgattgg tattttgtgg ggtaccatgc tggcggtgat gcgcttgtcc
    41161 agcttcgcgc ctgtttcgtg gtttgccaaa gcctacgtta acgtattccg ctcgattcct
    41221 ttagtcatgg ttttgctgtg gttttacttg attgtgccgg gatttctgca aaatgtgctg
    41281 ggattatcgc caaaaaatga tattcgttta atttcggcga tggtggcgtt ttcgatgttt
    41341 gaagcggcct actattcaga gattatccgt gccggtattc aaagtatttc ccgtggtcag
    41401 tcgagcgccg cgctggcgtt ggggatgact cactggcagt cgatgaaact gattattctg
    41461 ccgcaggctt tccgcgcgat ggtgccgctg ctgctcactc agggcatcgt actgttccag
    41521 gatacctcgc tggtgtatgt gttatctctg gccgatttct tccgtaccgc ctcaaccatt
    41581 ggtgagcgtg atggtacgca ggtcgagatg atcctgtttg ccggatttgt ttatttcgtt
    41641 attagcctta gcgcgtcgtt gttggtcagc tacttgaaaa gaaggacagc ataatgatta
    41701 ccctgaaaaa tgtttcaaaa tggtatggtc actttcaggt gctgaccgac tgctcaaccg
    41761 aagtgaaaaa aggcgaagtg gtggtggttt gcggcccgtc tggttccggc aaatcaacgc
    41821 tgattaaaac cgtcaacggc ctcgaaccag tgcagcaagg tgaaatcacc gtcgatggta
    41881 tcgtggttaa cgacaagaaa accgatctgg caaagctgcg ctcccgcgtc gggatggtat
    41941 tccagcattt cgagctgttc cctcatctgt cgattatcga aaacctgacc ctggcgcagg
    42001 tgaaagtgct taaacgcgat aaagcgcccg ctcgtgaaaa agcattgaaa ttgctggagc
    42061 gcgtgggact ttccgcacat gccaataaat ttccggcgca gctttccggc ggtcagcagc
    42121 agcgcgtggc aatcgctcgc gcattgtgta tggctcctat tgcgatgctg ttcgacgaac
    42181 cgacatcggc gctggatccg gagatgatca acgaagtgct ggacgtgatg gtggaactgg
    42241 cgaacgaagg aatgaccatg atggtggtga cccacgaaat gggctttgcc cgcaaagtgg
    42301 cgaatcgggt gatctttatg gacgagggta aaattgtcga agactcgccg aaagacgctt
    42361 tcttcgatga tccgaaatcg gaccgcgcaa aagacttcct cgcgaaaatc ctgcattaat
    42421 cactctggcg cacataccca attgcgcgcc atctcacgct ttatcgtttc gcccctcgta
    42481 tctgttacat cctggcgtta cccttgtcgc aaagaagcac acaacaagga gcaacaatgg
    42541 cactgccaat tctgttagat tgcgacccag gtcatgatga cgctatcgca atagttctcg
    42601 cactcgcctc accagagctt gatgtcaaag caattacgtc ttccgccgga aaccagacac
    42661 cagaaaaaac cttacgcaat gttctgcgta tgctgacctt gcttaatcgc accgatattc
    42721 cggtggcagg cggcgcggta aaaccgttaa tgcgtgagtt gattatcgcg gacaatgtgc
    42781 acggcgaaag cggtctcgac ggcccggcat taccggaacc gacgttcgca ccgcaaaact
    42841 gtacggcggt ggagctgatg gcgaaaacgc tgcgcgaaag tgcggaacct gtcacgattg
    42901 tttctaccgg accgcaaact aacgttgcct tgctgctcaa tagccatccg gaactgcata
    42961 gcaaaattgc ccgtatcgtg attatgggcg gcgcaatggg gcttggtaac tggacgcctg
    43021 cggctgaatt taatatttac gttgacccgg aagcggcaga aattgtcttc cagtcaggga
    43081 tcccggtggt gatggccggt ctggatgtta ctcataaagc gcaaatccac gttgaagaca
    43141 ccgagcgttt ccgcgcgatt ggcaaccctg tttcaaccat tgttgccgaa ctgctggatt
    43201 tcttcctcga atatcataaa gacgaaaaat ggggctttgt cggcgcaccg ctgcatgacc
    43261 catgcaccat cgcctggctg ttgaaacccg agttgtttac cactgttgag cgctgggttg
    43321 gcgtggaaac acaggggaaa tatacccagg gtatgacggt tgttgattat tattatctga
    43381 ctggcaataa accgaatgcc actgtaattg tcgatgttga tcgtcagggc tttgttgatt
    43441 tactggctga tcgtctgaaa ttttacgctt aacactcgtc attgattaag atcaccacgc
    43501 gttacctggg tggtcttaat ctatttatct ctttttcttt caagatataa atgcaatgga
    43561 taatgcagaa ctcgccatag gtatcgatct cggcactacc aatagtttaa ttgccgtctg
    43621 gaaagacggt gccgcgcaat taattccaaa taagttcggt gaatatttaa caccatccat
    43681 aattagcatg gatgaaaata atcatatttt agtcggaaaa ccggctgtat cacggcgtac
    43741 ttcgcatccg gataaaacgg cagcgttatt taaacgtgca atgggcagta ataccaactg
    43801 gcggttaggc agcgacacat ttaacgcgcc agaactgtcc tctttggtat tacgctcatt
    43861 aaaagaagat gccgaagaat ttctgcaacg tccgattaaa gatgtggtga tctccgttcc
    43921 ggcttatttc agcgatgaac aacgcaagca tacccgttta gcagcggact tagccgggtt
    43981 aaatgcggta cgcttaatta atgaacccac agcagctgcg atggcgtatg gcctgcatac
    44041 ccaacaaaat acccgttcgc tggtgtttga tctcggtggc ggcacgtttg acgttacggt
    44101 gcttgagtac gccacgccgg tgattgaagt tcacgcctcc gctggcgaca acttcctcgg
    44161 tggcgaagat tttacccata tgttggtcga tgacgtttta aagcgcgcgg atgtcacaag
    44221 gaccacgctt aacgagagtg aactggcagc cttgtacgcc tgtgtggaag cggcaaaatg
    44281 tagcaatcaa tcgccaatgc acattcgctg gtagtaccag gatgaaacgc gggaatgcga
    44341 attttacgag aacgaactgg aagatttgtg gttgccgctg ctcaatcgct tgcgagtgcc
    44401 gattgaacag gcgttgcgcg atgcgcgtct gaagccgagt caaatcgaca gtctggtgct
    44461 ggttggcggc gcgtcacaaa tgccgctggt gcagcgaatc gccgtgcgtc tgtttggcaa
    44521 attaccgtat caaagttacg atccgagcac cattgtcgcg ctgggcgcag caatccaggc
    44581 cgcctgccgc ttacgcagtg aagatattga agaagtgatc ctcactgata tttgccctta
    44641 ctcgttgggc gttgaggtta accgccaggg cgtttccggc attttctcgc cgattattga
    44701 acgaaacacc actgtgcccg tgtcgcgtgt agaaacttat tcaaccatgc acccggaaca
    44761 ggattcaatt acggttaacg tctatcaggg agaaaaccac aaagttaaaa acaacattct
    44821 ggtggaatcc ttcgatgtgc cgttgaagaa aaccggtgct tatcagtcga ttgatattcg
    44881 ctttagttat gatatcaacg ggttgcttga agttgacgtg cttctggaag acggcagcgt
    44941 taagtccaga gtgattaacc acagcccggt aacattgagc gcgcagcaga ttgaagagag
    45001 tcggacgcgg ttatccgcat tgaaaattta tccgcgcgat atgctcatca atcgcacctt
    45061 taaagccaaa ctggaagagt tgtgggcgcg ggcgctgggt gacgagcgag aagagattgg
    45121 ccgggtgatc accgattttg atgcggcgct gcagtcaaac gatatggccc gcgtcgatga
    45181 ggtcaggcgg cgggcgagcg tttatttagc cattgagacc tcataatcac ggctgccgga
    45241 tgcggcgtaa atgctgcatc cggcatcggc gcaaaaaaca catcgtcaac taaacaacat
    45301 cgcgattttg ccgagagcat aacctataac cagcaccgtt aaactgattt tgataatgct
    45361 ggtaatgctc tgtggttttt caccctcatc atttttttct tcatggtttt caggtgttgg
    45421 ctcatcgaca tcttcatcct ccggctcgag ttttactttc gtcaacagca tccgggtgat
    45481 aatggcctct aatggatgag catctccgcc ttccagcctt gtattaagac gggccagcat
    45541 ctcttgtttc ggaaacgctt caagtactac ctgttccgct acccgataca aatccagtaa
    45601 cggtgaatct tcttctgtct cgctaccgac tctcgatttc caccacgccc atgctttgcg
    45661 tttgaagaat aacgactcaa taaacgattg acctaacggc gataattttc cgctatgtaa
    45721 aatttccgcc cataacagcc gcgtccgggc gctctcacca ggaacatatt tcagcggttg
    45781 accttgcgca tctaccatgc tccgcgcttc gataaccatc actaataacg cgaaataatc
    45841 cgggcaatga cgcttgctcc actgcaacaa caaattatca gcttgtgtag atgggtaact
    45901 ttcccagtac gcgccgagat cagcaagcag gctttccccc tcgccgcagt acaaacgttg
    45961 tgcacactgg taataccggg catcttcgct ttctggttct tccgcttgcc actgtttggc
    46021 tacaggaatg agttccacca tacctgtttg caccgagctg taccagcgca acagtttgcg
    46081 atgtaacttt gcatcatcag gaatgaacca cggtccttcc atcgccaaaa acgcagtgac
    46141 atagtcagga tggtaacggc agcaacgttc cagcgcgaag taatattcaa cagtctggtt
    46201 ctgtaacgcg acgggatatt ccagaaggct aaggaaatcg aaacaatcac cagcctgaat
    46261 ggcctcaaga aaagcctccc tttcttcctc atccattcct tcgctgtcat taaacgaatg
    46321 ccagttcaaa cgctctgcca acaaagaagc acaatttaat gacagatatc gcgcttctat
    46381 agcgattgca cacagcggcc agcgtaattg atcgacatcc tccatgttcc aggtatttaa
    46441 ttgctgaata aatttctgcc aggcggaggg ctgaaaacga tcactttctg aatccagtaa
    46501 tgtcctgaat gcacgtagta tttcatgttc agcggtggca tctttttctt catcatctgc
    46561 ttcctcgaca ggatttaccg ccagtcgcag cgcctcttca taggcctggc gtaatgcttt
    46621 aaacccctgc ggatcggttt cgggatgaca caacggtaag cgagcaagat aagcctggcg
    46681 gataatgtct atttgcgtcg tgctttcaat ttcgaggatt tgccaacatg ttttcatagg
    46741 tcgctcacat ccttaagcgt taccgggcat tcaggctgga cagttaatgg ctgccaggga
    46801 agcgtggcat agaaacggtc gcttcccgct gccagctctt cgaacatccg actcccggcg
    46861 ctatcgtatt tgtacagggt tagcgcctcg cgtagcgaag cgttgtcttc gcactgagaa
    46921 gattgccagt agagacgccc tagtgaatag gcagaaaaat agtgcatcca gctgtgatag
    46981 taatggtgag ctcgtgcata gacgcgcgat tgcagccata aactctcttc ttccgtcagc
    47041 cagttgttga gcacaccggc ccgtgacaga aaccccattc gaacataatc ccaggctttg
    47101 attccccctt ctccacagca catagcggtt tgggcaacga attgggtata aatcaaccct
    47161 ctctccgagc caccagcaat taatgctttc cactcttcag gggaagagcg ttgccatgtc
    47221 agatataagc cagctaaatc attggcatga ccatcatcag tcatctgata aatagtctga
    47281 attaaatcat cacgattatt tattccccag gatgatgaga gatcgaattc ttttatcccg
    47341 gtatacattt ttggcgagtc atgcgcgggt ttgcaactct gattaagtat ttccatgggc
    47401 gcagaaagcg caaaaagaag atattgttct tccttattca ttttttaata atcccttaag
    47461 tgcattaact gactactttt ttattattga tgagtataaa cttgacgctt tcaaaataaa
    47521 aagaaaatcg aagcattcac acatgaataa aaaattaatg tatatattcg caatttttat
    47581 agttgcagca attacctgta ttagccaacc caagaaaacg atgttgcgtg ataaagccat
    47641 ggtgaattat gcctttgatt atttaagatc accaggcagt ctaccgttaa ccacggcagc
    47701 cacggagctt tccgcgattc atggtcactc aacatcgcaa tatcgccttg gcgaatttta
    47761 tcttcatggt agcgacggta aaccactgga ttatacacag gcgagatact ggtatgagca
    47821 atcagcagaa caggaaaatc cgcgcgcaca aagcaaactg gggtggatct acctgaaagg
    47881 cctgggagtc aaacccgata cccgtaaagc aattctctgg tataaggaag cagctgaaca
    47941 agggtatgct catgctcaat atactttggg tttgatctac agaaatggca ctggtattaa
    48001 tgttaaccat tatgaatctc aaaaatggtt aaaactggct gccaaacaac attacaaaaa
    48061 tgcggaaaga ttacttgccg gacttcccgc acattaacgt cgactgtcac ccgaagagat
    48121 ggagtatttt ccctatcaaa ccggcaaaga taaaaatgta gaaaataatt ttgatgacgc
    48181 ccagacagcc cagcccgcca tcatcaacgg gcattggctg cggttgcggc tccggctcga
    48241 cggtttcctg accggtaaaa ttcgccgcct gaattaataa tcgacgaata attaatgctt
    48301 ccagtggaca acgatcttct gcatcaaggc ggctcacaat cggcattaat gattcttctg
    48361 tcggttggct ggcctgcact aattgtttgg caactaaata taatggccat aagggatgtt
    48421 ctgtatcacg cgtgagtgag atccgcggct tacgtccttt gtgcaaaacc atctcgacca
    48481 gtgaacgccc cagcgcagag agtttttcat cgctgagtat ttcgtataaa tgaaaacgcg
    48541 tacgggcgct gtcgccgggg acataaagta gcggttttcc ctgatcgtta accagatcac
    48601 gcgcttcaat catcatcacc actaatgggt aataatagac ccgatgctga cggcaccatt
    48661 gcaacattaa agcatccgcc tgggtggagg gatactcgcg ccagtagtaa caaagttctg
    48721 ccagcaggct ttcgccttct ccacaataga cgcgttgtgc gtattcataa taacgcgcat
    48781 cttcattatc aggttcagct tcccgccacg accgggcgta atccaggagt tcggggattc
    48841 ccaggcggct tgcgctatac cagcggagta aatttctgtg cagttttgca tcatcgggga
    48901 taatcactgc gcgatgttgc gctaaataca atgtcagcca ttctggatgc gatgaccaaa
    48961 tccgagcgaa catttggtaa aaatcgatgg tgtcattttg cacggcaact ggcagatgca
    49021 gaatggtctg gaagttaaaa acattcccct tcgcaatggc atataaaaag gattccagtt
    49081 cttcttcgtc tatttccccg acgttttcct cctgcaacca ccgcaatctt tctgctaata
    49141 acaccacgca ctcgaaggat aaatgggcag tgttcatggc gattgtgcac agcgaccagc
    49201 gtaattcatc aatctcttcc atcgagcaat aatttaattg ctgaatgaat cgctgccagg
    49261 cggaggacag aaaacgttcg ctatcagagg caagtaacgc gcgaaaggcg agcagaattt
    49321 catgttctgc tacctcatat tcttctggtt gccaaacaga tttagcaggc gactgcgcaa
    49381 tccgtagcgc ctcctcatac gcctgacgaa gttgtttaaa accctgcgga tcggtttctg
    49441 gatgaaagga cggtaacagc gccagataag cgcggcggat aatatcgacg tcagtcgttt
    49501 cctctatatc gaggatcttc cagcaatttt tcataagtcg ctcatatcct taagcgtttc
    49561 cgggcatgtg gggtagtcag ccagtggttg ccaggacaag gtagggtaat acgcatcatc
    49621 actggcaaat aattgataaa acatatcatt gccggaatcg tcgtattctt tacgcgctaa
    49681 gaattcgcgt aataaaggca gatgatcatc ttcgggagac tgccaatatt gccgaccaaa
    49741 ggtataaccg gcaaagtatt gccgccagtt actataataa cgtagagccc gtagatgaat
    49801 acgcgactgg atccatagac tttcttcttc actcaaccag ttattaagta cgcccattcg
    49861 acttaaaaaa cccatccgga cataatccca tgctttaatt cctccctcac cacagcattc
    49921 ggctgtgctg gcgacaaatt gcgcataagc ctgaccttgc tcatttaatt cagcaagata
    49981 gtcacgccat tcacatggag aataacgaaa ccagcggtga taaaaccctg ccaaccgagc
    50041 ggcatgaccg ttctcgatca ttcggtgaat tatgcgcagc aggtcatcac ggtcatcaat
    50101 tccccatgag gaagaaaggt caaaaaagca tgtacctaaa tacattttgg gggatgaatg
    50161 cgaaggtaaa cagcattcat tgcgtatttc catcggagtg gaaagtgcaa acaatatttt
    50221 ttgcgacccc atgtccatat aatttatcct taaacattca tttaatgaat gtaaactaca
    50281 tttcgaggct cagttattta tacttttctg acgcaacata atacgcataa aataaggctt
    50341 acgcaaaaat ggaatacata aaaatataat atattttatc tacaaccata aggaaatgat
    50401 aatgattttt acgtcaagtt gctgcgataa tttatcaata gatgagatta tcgaacgtgc
    50461 tgaaaaaggc gattgcgagg ctcaatatat tgttgggttt tattataatc gcgatagcgc
    50521 aattgactct ccggacgacg aaaaggcctt ttactggctg aagctggccg ctgagcaagg
    50581 tcattgtgaa gcacagtatt ccttagggca aaagtatacc gaggataaaa gccgtcataa
    50641 agataatgag caagccatct tctggctgaa aaaagctgcc ctacaaggcc atactttcgc
    50701 ttccaacgcc cttggctgga tactggatcg tggagaagac cccaactata aagaagcggt
    50761 tacctggtat cagatagccg cggagagcgg aatgtcttat gcgcaaaata atcttggatg
    50821 gatgtacaga aatggcaacg gagtcgcaca agactatacg ctggcatttt tctggtacaa
    50881 acaagctgca ttacaaggcc atagttacgc acaagacaat ctggccgatc tttataaaga
    50941 cggagaaggc gttgctcaaa acaagacact cgccgcattc tggtatttga aaagcgcaca
    51001 gcagggtaat cggcacgccc agtttcaaat tgcatgggat tataacgctg gcgaaggggt
    51061 ggaccaggac tataagcaag cgatgtactg gtatctgaag gctgccgctc aggaaagcgt
    51121 cggcgcttac gtcaacatcg gttatatgta taaacacgga caaggcgttg agaaagatta
    51181 tcaggctgcc tttgaatggt ttacgaaagc cgctgaatgc aatgacgcca ctgcctggta
    51241 taacctggcc attatgtatc actacggaga aggaagacct gtcgatctcc gacaggctct
    51301 cgacctgtat cgtaaagttc agtcatccgg aaccagagat gtcagtcagg aaattcgtga
    51361 gactgaagct ttactgtaga aataaaaaca agggccgggg aatttcattc tgcccatatt
    51421 ctctttattg ctgtgcgaaa tcagactatt acggctcaaa cgggcgtctc tggaactggt
    51481 cataaccaca tttcgggcat agcgtcagca cttccggtgt gtagatcggg agatggaagt
    51541 gacatttctc gcagaccaga tttcccagcc cgaccacttc tccgctgtga taaaccccat
    51601 gatgattgag gtcctggaaa atttcgcgcc attcaagctg cgttttatcg gtgatgtctg
    51661 ccagctcctg ccacaagctt tctttaatca cccgcataaa gacgctgtca gattcttctt
    51721 tcaggctctc ttcatagctc atggcgaact cttccaggtc acgtctgaca gctcgcgtca
    51781 gctcatcgat ctcggttcgc gttaactccc ctgtttttat tacgcgctcg cgcgcctgtt
    51841 ccaccagtgc gtcgatatca cgttcgccat tgcgcaggcg ttcgttcagt gacgcgacca
    51901 gttcacggta atattgagca accttgttca tcgtttcatc tcccgggtcg ttaactgact
    51961 ataataatag acgttctttg ttccttgctt tgctaatacg cccacaaact ctgtaaatta
    52021 atgtacgaat aatccgggca tcatttcaac gtcgagtgcg cgaaaggctg ttttgacgcg
    52081 gacgtttggg ctatgctatg cggatctgaa aaaccacctc aacgctacat ttgtagccgt
    52141 attgaaaaca ggaccactgg ctgccatgca agagcaatac cgcccggaag agatagaatc
    52201 caaagtacag cttcattggg atgagaagcg cacatttgaa gtaaccgaag acgagagcaa
    52261 agagaagtat tactgcctgt ctatgcttcc ctatccttct ggtcgactac acatgggcca
    52321 cgtacgtaac tacaccatcg gtgacgtgat cgcccgttac cagcgcatgc tgggcaaaaa
    52381 cgttctgcag ccgatcggct gggacgcgtt tggtctgcct gcggaaggcg cggcggtgaa
    52441 aaacaacacc gctccggcac cgtggacgta cgacaacatc gcgtatatga aaaaccagct
    52501 caaaatgctg ggctttggtt atgactggag ccgcgagctg gcaacctgta cgccggaata
    52561 ctaccgttgg gaacagaaat tcttcaccga gctgtataaa aaaggcctgg tatataaaaa
    52621 gacttctgcg gtcaactggt gcccgaacga ccagaccgta ctggcgaacg aacaagttat
    52681 cgacggctgc tgctggcgct gcgataccaa agttgaacgt aaagagatcc cgcagtggtt
    52741 tatcaaaatc actgcttacg ctgacgagct gctcaacgat ctggataaac tggatcactg
    52801 gccagacacc gttaaaacca tgcagcgtaa ctggatcggt cgttccgaag gcgtggagat
    52861 caccttcaac gttaacgact atgacaacac gctgaccgtt tacactaccc gcccggacac
    52921 ctttatgggt tgtacctacc tggcggtagc tgcgggtcat ccgctggcgc agaaagcggc
    52981 ggaaaataat cctgaactgg cggcctttat tgacgaatgc cgtaacacca aagttgccga
    53041 agctgaaatg gcgacgatgg agaaaaaagg cgtcgatact ggctttaaag ctgttcaccc
    53101 attaacgggc gaagaaattc ccgtttgggc agcaaacttc gtattgatgg agtacggcac
    53161 gggcgcagtt atggcggttc cgggtcacga ccagcgcgac tacgagtttg cctctaaata
    53221 cggcctgaac atcaaaccgg ttatcctggc ggctgacggc tctgagccag atctttctca
    53281 gcaagccctg actgaaaaag gcgtgctgtt caactctggc gagttcaacg gtcttgacca
    53341 tgaagcggcc ttcaacgcca tcgccgataa actgactgcg atgggcgttg gcgagcgtaa
    53401 agtgaactac cgcctgcgcg actggggtgt ttctcgccag cgttactggg gcgcgccgat
    53461 tccgatggtg acgctggaag acggtaccgt aatgccaacc ccggacgacc agctgccggt
    53521 gatcctgccg gaagatgtgg tgatggacgg cattaccagc ccgattaaag cagatccgga
    53581 gtgggcaaaa actaccgtta acggtatgcc agcgctgcgt gaaaccgaca ctttcgacac
    53641 ctttatggag tcctcctggt actatgcgcg ctacacttgc ccagagtaca aagaaggtat
    53701 gctggattcc gaagcggcta actactggct gccggtggat atctacattg gtggtatcga
    53761 acacgccatt atgcacctgc tctacttccg cttcttccac aaactgatgc gtgatgcagg
    53821 catggtgaac tctgacgaac cagcgaaaca gttgctgtgt cagggtatgg tgctggcaga
    53881 tgccttctac tatgttggcg aaaacggcga acgtaactgg gtttccccgg ttgatgctat
    53941 cgttgaacgt gacgagaaag gccgtatcgt gaaagcgaaa gatgcggcag gccatgaact
    54001 ggtttatacc ggcatgagca aaatgtccaa gtcgaagaac aacggtatcg acccgcaggt
    54061 gatggttgaa cgttacggcg cggacaccgt tcgtctgttt atgatgtttg cttctccggc
    54121 tgatatgact ctcgaatggc aggaatccgg tgtggaaggg gctaaccgct tcctgaaacg
    54181 tgtctggaaa ctggtgtacg agcacacagc aaaaggtgat gttgcggcac tgaacgttga
    54241 tgcgctgact gaagatcaga aagcgctgcg tcgcgatgtg cataaaacta tcgctaaagt
    54301 gaccgatgat atcggccgtc gtcagacctt caacaccgca attgcggcga ttatggagct
    54361 gatgaacaaa ctggcgaaag caccgaccga tggcgagcag gatcgcgctc tgatgcagga
    54421 agcactgcag gccgttgtcc gtatgctcaa cccgttcacc ccgcacatct gcttcacgct
    54481 gtggcaggaa ctcaaaggcg aaggcgatat cgacaacgcg ccgtggccgg ttgctgacga
    54541 aaaagcgatg gtggaagact ccacgctggt cgtggtgcag gttaacggta aagtccgtgc
    54601 caaaatcacc gttccggtgg acgcaacgga agaacaggtt cgcgaacgtg ctggccagga
    54661 acatctggta gcaaaatatc ttgatggcgt tactgtacgt aaagtgattt acgtaccagg
    54721 taaactcctc aatctggtcg ttggctaagc gcgggaggaa gcgtgcgata tctggcaaca
    54781 ctgttgttat ctctggcggt gttaatcacc gccgggtgtg gctggcatct gcgtgacacc
    54841 acgcaggttc cttccactat gaaggtcatg atcctggact caggcgatcc gaacgggcca
    54901 ttaagccgtg cggtgcgtaa ccagttacgt ctgaatggtg tcgagttgct tgataaagaa
    54961 accacgcgta aggacgttcc atccctgcgt ttgggtaaag tgagcatcgc gaaagatacc
    55021 gcatcggtat tccgtaacgg tcaaacagca gagtatcaga tgatcatgac ggtgaatgcg
    55081 accgtgttga tccccggccg tgatatctac ccgattagcg ccaaagtctt ccgttcattc
    55141 ttcgataacc cgcaaatggc gttagcgaaa gataacgaac aagacatgat cgtaaaagag
    55201 atgtacgacc gtgctgccga acagctgatt cgtaagctgc caagcatccg tgctgcggat
    55261 attcgttccg acgaagaaca gacgtcgacc acaacggata ctccggcaac gcctgcacgc
    55321 gtctccacca tgctgggtaa ctgatgattc ggttgtaccc ggaacaactc cgcgcgcagc
    55381 tcaatgaagg gctgcgcgcg gcgtatcttt tacttggtaa cgatcctctg ttattgcagg
    55441 aaagccagga cgctgttcgc caggtagctg cggcacaagg attcgaagaa caccacactt
    55501 tttccattga tcccaacact gactggaatg cgatcttttc gttatgccag gctatgagtc
    55561 tgtttgccag tcgacaaacg ctattgctgt tgttaccaga aaacggaccg aatgcggcga
    55621 tcaatgaaca acttctcaca ctcaccggac ttctgcatga cgacctgcta ttgatcgtcc
    55681 gcggtaataa attaagcaaa gcgcaagaaa atgccgcctg gtttactgct cttgcgaatc
    55741 gcagcgtgca ggtgacctgt cagacaccgg agcaggctca gcttccccgc tgggttgctg
    55801 tgcgcgcaaa acagctcaac ttagaactgg atgacgcggc gaatcaggtg ctctgctact
    55861 gttatgaagg taacctgctg gcgctggctc aggcactgga gcgtttatcg ctgctctggc
    55921 cagacggcaa actgacatta ccgcgcgttg aacaggcggt gaatgatgcc gcgcatttca
    55981 ccccttttca ttgggttgat gctttgttga tgggaaaaag taagcgcgcg ttgcatattc
    56041 ttcagcaact gcgtctggaa ggcagcgagc cggttatttt gttgcgcaca ttacaacgtg
    56101 aactgttgtt actggttaac ctgaaacgcc agtctgccca tacgccactg cgtgcgttgt
    56161 ttgataagca tcgggtatgg cagaaccgcc ggggcatgat gggcgaggcg ttaaatcgct
    56221 taagtcagcc gcagttacgt caggccgtgc aactcctgac acgaacggaa ctcaccctca
    56281 aacaagatta cggtcagtca gtgtgggcag agctggaagg gttatctctt ctgttgtgcc
    56341 ataaacccct ggcggacgta tttatcgacg gttgatatga aatctttaca ggctctgttt
    56401 ggcggcacct ttgatccggt gcactatggt catctaaaac ccgtggaaac gctggcgaat
    56461 ttgattggtc tgacgcgggt cacaatcatc cctaataatg ttcctccgca tcgtccccag
    56521 ccggaagcga acagcgtgca gcgtaaacac atgcttgaac tggcgattgc cgacaagcca
    56581 ttatttactc ttgatgaacg cgagctaaag cgcaatgccc cctcttacac tgcgcaaaca
    56641 ctgaaagagt ggcggcagga acaaggaccg gacgtgccgc tggcgtttat tattggtcag
    56701 gattcattgc tgacctttcc gacctggtac gaatacgaaa cgatactcga caattcacat
    56761 ttgatcgtct gtcggcgtcc aggttaccca cttgaaatgg cgcaaccgca ataccagcaa
    56821 tggctggaag atcatttgac acataacccg gaagatcttc accttcagcc tgccggtaaa
    56881 atttatctgg ctgaaacgcc gtggtttaac atctcggcga ccatcatccg cgaacgtttg
    56941 caaaacggtg aatcgtgtga ggatttattg ccggaaccgg tattgactta cattaaccaa
    57001 caaggcttgt atcgctgata cctgtcgtta cggagggcat gatgcgactg tggttaattc
    57061 gtcatggcga aacacaagcg aatgtcgatg gtctgtacag cggtcatgcg cccacacccc
    57121 tgaccgcacg cggtattgag caagcgcaaa atctgcatac actgctagat gatgtttcct
    57181 ttgacctggt tttatgcagt gaactggaac gggcacagca taccgcgcga ctggttctca
    57241 gtgaccgcca gctccccgtt catatcatcc ctgaactcaa cgaaatgttt tttggcgact
    57301 gggagatgcg acatcatcgc gacctcatgc aagaagatgc cgaaaactat agcgcgtggt
    57361 gcaatgactg gcagcatgct atccccacga acggtgaagg atttcaggca ttttcgcaac
    57421 gtgtggaacg ctttatcgca aggcttagtg aatatcagca ctatcagaat attttaatcg
    57481 tcagccatca gggcgtactg agtctgttaa ttgctcgttt aattggcatg cctgccgagt
    57541 ctatgtggca ttttcgcgtt gaccaggggt gttggagcgc cattgatatc aaccaaaaat
    57601 tcgcgacgct acgcgtcctc aatagccgtg ccatcggggt cgagaacgca tgacttttct
    57661 gtttttttac gggtaagccg caacgaccat tgacagcccc gggcaggctg atattctccg
    57721 cagccagact ttttccgcca gacacgactt tgtagaaatt gttttacaaa aatggcgatg
    57781 caatctgcgg cgcggggtgg gatgatagcc cactttcgaa agccgattcg gcgacaattg
    57841 tcccgaaatc gcctctggtt caggtatact gacagaccat ttttatctat ttgattcacc
    57901 cagggggaaa acttgcaggg taaagcactc caggattttg ttatcgacaa aattgatgac
    57961 ctcaaaggtc aggacatcat cgccttagac gttcaaggca aatccagcat caccgactgc
    58021 atgatcatct gtacgggtac gtccagccgt catgttatgt ccattgctga ccacgttgtg
    58081 caggagtctc gcgcagcggg cctgttaccg ctcggcgtag aaggtgaaaa cagcgccgac
    58141 tggattgtcg tggatttggg cgatgtgatt gtccatgtca tgcaggaaga gagccgtcgc
    58201 ctgtatgaac tggaaaaact ctggagttaa tgcgtgaagc tgcaacttgt cgccgtggga
    58261 acgaaaatgc cggactgggt acaaaccggt tttaccgagt acctgcgtcg ttttccgaaa
    58321 gatatgccct tcgagctgat tgaaattccg gccggcaaac gcggcaagaa tgcggacatc
    58381 aagcgcatac tcgacaaaga gggtgagcag atgttggcgg ccgcaggcaa aaaccgcatt
    58441 gtcaccctcg atattccagg caagccctgg gatacgccgc agttagccgc tgagctggaa
    58501 cgctggaagc tggatggtcg cgacgtaagt ctactgattg gcgggcctga agggttgtcg
    58561 cctgcctgta aagcggcggc tgagcagagc tggtcgctgt cggcgcttac cctcccccat
    58621 ccgctggttc gcgtgctggt cgcagagagt ctgtaccggg cgtggagcat caccaccaac
    58681 catccttatc accgtgagtg ataagggagc tttgagtaga aaacgcagcg gatgaaacta
    58741 cataactctt ttcgcgacta tacggcagag tccgcgctgt ttgtgcgccg ggcgctggtc
    58801 gcctttttgg ggattttgct gctgaccggc gtgcttatcg ccaacctgta taatctgcaa
    58861 attgttcgct ttaccgacta ccagacccgc tctaatgaaa accgcattaa gctggtgcct
    58921 atcgcgccca gccgcggcat tatctacgac cgtaacggta tccctctggc cctcaaccgc
    58981 actatctacc agatagaaat gatgccggag aaagtcgata acgtgcagca aacgctggac
    59041 gctttgcgca gcgtggtaga tctgaccgat gacgatattg ctgcattccg aaaagagcgc
    59101 gcacgttcac accgtttcac ctctattccg gtgaaaacta acctaaccga agtacaagta
    59161 gctcgctttg ccgtcaatca gtaccgtttt ccgggtgtcg aagttaaagg ctataaacgt
    59221 cgttactatc cttacggttc ggcgttgacc cacgtcatcg gctatgtgtc gaaaatcaac
    59281 gataaagacg tcgaacgcct gaataatgac ggcaaactgg ccaactatgc ggcaacgcat
    59341 gatattggta agctgggcat tgagcgttac tatgaagacg tgctgcacgg tcagaccggt
    59401 tatgaagagg ttgaagttaa caaccgtggg cgtgttatcc gccagttaaa agaagtacca
    59461 ccgcaagccg gacacgatat ttacctgacg ctggatctca aactccagca atatattgaa
    59521 acgctgctgg cgggtagccg cgcagctgtg gttgttaccg aaccgcgtac aggtggggtg
    59581 ctggcgctgg tttccacgcc tagttatgac ccaaacctgt ttgttgacgg tatctccagc
    59641 aaagattatt ccgccttgct gaacgacccg aatacaccgc tggtgaaccg cgccacacag
    59701 ggggtttatc ctcccgcgtc tacagttaaa ccctatgtgg cggtttcggc attaagcgcc
    59761 ggggtgatca cgcgcaatac gacgctgttt gacccaggct ggtggcaact accagggtcg
    59821 gaaaaacgtt atcgtgactg gaaaaaatgg ggccacgggc gtctgaatgt cacaagatcg
    59881 ctggaagaat ctgcggatac cttcttctat caggtggcct acgatatggg gatcgatcgc
    59941 ctctccgaat ggatgggtaa attcggttat ggtcattaca ccggtatcga cctggcggaa
    60001 gaacgttccg gcaacatgcc tacccgcgaa tggaaacaga aacgctttaa aaaaccgtgg
    60061 tatcagggtg acaccattcc ggttggtatc ggccagggtt actggaccgc gaccccaatc
    60121 cagatgagta aggcactgat gatcctgatt aatgacggta tcgtgaaggt tccgcatttg
    60181 ctgatgagca ccgccgaaga cggcaaacag gtgccatggg tacaaccgta tgaaccgccc
    60241 gtcggcgata ttcattccgg ttactgggag ctggcgaaag acggtatgta cggtgttgct
    60301 aaccgcccta atggtacggc gaataaatac tttgccagcg caccgtacaa aattgcggcg
    60361 aaatctggta ccgctcaggt cttcggtctg aaagcgaacg aaacctataa tgcgcacaaa
    60421 attgccgagc gtttacgtga ccacaaactg atgaccgcct ttgcgccata caacaatccg
    60481 caagtggctg tcgccatgat tctggagaac ggcggcgcgg gtccggcggt tggtacgctg
    60541 atgcgccaga tcctcgacca cattatgctg ggtgataaca acaccgatct gcctgcggaa
    60601 aatccagcgg ttgccgcagc ggaggaccat taatcatgac ggataatccg aataaaaaaa
    60661 cattctggga taaagtccat ctcgatccca caatgctgct gattttactg gcattgctgg
    60721 tttacagcgc cctggttatc tggagcgcca gcggtcagga tattggcatg atggagcgta
    60781 aaatcggcca aatcgcgatg ggtctggtca tcatggtggt gatggcgcaa attcctccac
    60841 gcgtttatga aggctgggcc ccctatctct atatcatctg tattattttg ctggtggcgg
    60901 tagatgcttt cggagccatc tctaaaggcg ctcaacgctg gctggacctc ggtattgttc
    60961 gttttcagcc gtcggaaatt gccaaaatag ccgtaccact gatggttgcg cgctttatca
    61021 accgcgacgt ttgcccgcca tcgttgaaga atactggcat cgcgctggtg ctgatattta
    61081 tgcccacgct gctggtggct gcacagcctg acctgggaac atcaatcctc gttgcgcttt
    61141 ccggtctgtt tgtactgttc ctctctggcc ttagctggcg tctgattggc gtcgcagtag
    61201 tgctggtagc ggcgttcatt ccgattctgt ggttcttcct gatgcatgat taccagcgcc
    61261 agcgcgtaat gatgctcctg gacccggaat cagacccact cggcgcgggc tatcacatta
    61321 ttcagtctaa aattgctatt ggctccggcg gattacgcgg caaaggctgg ctgcacggca
    61381 ctcagtcaca gcttgaattt ctccccgaac gccatactga ctttatcttc gcggtactgg
    61441 cggaagagct gggattagtg ggcattctga ttctgctcgc tctctacatt ctgctgatca
    61501 tgcgcgggct gtggatagcc gccagagcgc aaaccacctt tggtcgcgtc atggctggtg
    61561 gcttaatgct gatattattc gtttatgtct tcgtaaatat tggtatggta agcggtattc
    61621 tgccggttgt aggggttccg ctcccactgg tcagttatgg aggatcggcg ctaattgtgc
    61681 tgatggctgg gttcgggatt gtaatgtcaa tccacaccca caggaaaatg ttgtcgaaaa
    61741 gcgtgtaaga ggtgcgcaat gcgtaagcag tggctcggga tctgcatcgc ggcaggaatg
    61801 ctcgcggcat gtacaagcga tgatggtcag caacagacgg taagtgtacc gcagcctgcg
    61861 gtatgtaacg gccccatagt tgaaattagc ggggcggacc cgcgtttcga accactgaac
    61921 gcgacggcaa atcaggatta ccagcgcgac ggtaaaagct acaaaatcgt gcaggatccg
    61981 tctcgattta gccaggcggg actggcggca atctatgatg ccgaaccagg cagtaacctg
    62041 acggcctctg gcgaagcttt cgatccgaca aagctgacgg cggcccatcc aacgcttccg
    62101 atccccagct acgccagaat cactaacctg gctaacgggc gaatgatcgt ggtgcgcatt
    62161 aatgatcgcg gcccttacgg caacgaccgc gttatttcgc tttctcgcgc ggcagctgac
    62221 cgtcttaaca cgtcaaacaa caccaaagtt cgtatcgatc cgattattgt cgcccaggat
    62281 ggttcgcttt ctggtcctgg tatggcttgt accacagtcg ccaaacagac ttacgccctg
    62341 cctgcacctc ccgatttaag cggtggcgcg ggaacaagtt cagtgtctgg cccgcagggt
    62401 gacattcttc cggtcagtaa ttcgacgcta aaaagcgaag atccgaccgg cgcgccggta
    62461 accagtagcg gtttcctcgg cgctccaacg accttagcgc ctagtgtact ggaaggcagc
    62521 gaaccgacgc ctgcgccaca gcccgttgtt acagcttcgt cgacaacgcc tgcaacctcg
    62581 cctgcaatgg tgacaccgca agccgcctcg caaagcgcca gcggcaactt tatggtgcaa
    62641 gtcggggccg taagcgatca ggctcgtgcg caacagtacc aacagcaact gggacagaag
    62701 ttcggcgtcc ccggtcgcgt aactcaaaat ggcgcggtct ggcggatcca gcttggccca
    62761 ttcgccagca aagccgaagc cagtaccttg cagcaacgtt tgcaaaccga agcccaatta
    62821 cagtcattta ttactaccgc gcagtagcgt aaagcaggca tctgatgtgt caatttctgt
    62881 aaatgacgtt aacaaagtca cgcggaaagt cagatgcctg ccggtagtgc atttgctata
    62941 gtagggcact tttttaattc catcacggat gtcgtagttc agaccatgaa taccattttt
    63001 tccgctcgta tcatgaagcg cctggcgctc accacggctc tttgcacagc ctttatctct
    63061 gctgcacatg ccgatgacct gaatatcaaa actatgatcc cgggtgtacc gcagatcgat
    63121 gcggagtcct acatcctgat tgactataac tccggcaaag tgctcgccga acagaacgca
    63181 gatgtccgcc gcgatcctgc cagcctgacc aaaatgatga ccagttacgt tatcggccag
    63241 gcaatgaaag ccggtaaatt taaagaaact gatttagtca ctatcggcaa cgacgcatgg
    63301 gccaccggta acccggtgtt taaaggttct tcgctgatgt tcctcaaacc gggcatgcag
    63361 gttccggttt ctcagctgat ccgcggtatt aacctgcaat cgggtaacga tgcttgtgtc
    63421 gccatggccg attttgccgc tggtagccag gacgcttttg ttggcttgat gaacagctac
    63481 gttaacgcac tgggcctgaa aaatacccac ttccagacgg tacatggtct ggatgctgat
    63541 ggtcagtaca gctccgcgcg agatatggcg ctgatcggcc aggcattgat ccgtgacgta
    63601 ccgaatgaat actcgatcta taaagaaaaa gaatttacgt ttaacggtat tcgccagctg
    63661 aaccgtaacg gcctgttatg ggataacagc ctgaatgtcg acggcatcaa aaccggacac
    63721 actgacaaag caggttacaa tcttgttgct tctgcgactg aaggccagat gcgcttgatt
    63781 tctgcggtga tgggtggacg tacttttaaa ggccgtgaag ccgaaagtaa aaaactgcta
    63841 acctggggct tccgtttctt cgaaaccgtt aacccactga aagtaggtaa agagttcgcc
    63901 tctgaaccgg tttggtttag tgattctgat cgcgcttcgt taggggttga taaagacgtg
    63961 tacctgacca ttccgcgtgg tcgcatgaaa gatctgaaag ccagctatgt gctgaacagc
    64021 agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg gaactatcaa cttccagctt
    64081 gatggcaaaa cgatcgagca acgcccgctg gttgtgttgc aagaaatccc ggaaggtaac
    64141 ttcttcggca aaatcattga ttacattaaa ttaatgttcc atcactggtt tggttaaaaa
    64201 ttgaacactt gaaagtgtaa tttccgtccc catatactaa gcatcagtaa aaaaactccc
    64261 gccttctggc gggagttgct atttaattac gttacgccgg agctgacatg aaaaccaaac
    64321 ttaacgaact gcttgaattc cctactcctt ttacttacaa agttatgggg caggcgttac
    64381 ctgagctggt tgatcaggtg gttgaagtgg tacagcgcca tgcgccaggt gactacaccc
    64441 caacggtaaa accaagcagc aaaggcaact accactcggt atctatcact atcaacgcca
    64501 ctcatatcga gcaggttgaa acactgtatg aagaactggg caaaatcgat attgtccgca
    64561 tggttctgta actcgcttct ccgttacccg gctcctggtc gggtaactcc cctctcctcg
    64621 ctgtgatata cttttccccc acttttactc attctccacg gagatgccgt tttgtatcag
    64681 gataaaattc ttgtccgcca gctcggtctt cagccttacg agccaatctc ccgggctatg
    64741 catgaattca ccgatacccg cgataatagt acccttgatg aaatctggct ggtcgagcac
    64801 tatccggtat tcacccaagg tcaggcagga aaagcggagc acattttaat gccgggtgat
    64861 attccggtga tccagagcga tcgcggtggg caggtgactt atcacgggcc gggacaacag
    64921 gtgatgtatg tgttgcttaa cctgaaacgc cgtaaactcg gtgtgcgtga actggtgacc
    64981 ttgcttgagc aaacagtggt gaataccctg gctgaactgg gtatagaagc gcatcctcgg
    65041 gctgacgcgc ctggtgtcta tgtcggggaa aagaaaattt gctcactggg tttacgaatt
    65101 cgacgcggtt gttcattcca cggtctggca ttaaacgtca atatggatct ttcaccattt
    65161 ttacgtatta atccttgtgg gtatgccgga atggaaatgg ctaaaatatc acaatggaaa
    65221 cccgaagcga cgactaataa tattgctcca cgtttactgg aaaatatttt agcgctacta
    65281 aacaatccgg acttcgaata tattaccgct taattccata catcaatggc ccaatttaca
    65341 ctgggtcatt actcataata cctgctaatg ctttacatct ccttcgcgtt tgtatatttt
    65401 ctttatcact tgcagaaatg cggtgccacg ccttgctccg taactcaccc cattgattaa
    65461 cctattgaaa tagttgatta tggtgagcaa aagcgttccg ttccaattag acataagatt
    65521 attgtaattt tttaaatatt atttttccat gaataaaatt ggagtaagtg cgtggatagt
    65581 aataatcaaa ttgaaccctg tttaagcaga aagtcgtcag aaggtaaacc acaaatattt
    65641 acaaccttac gaaatattga tcttaacctt ctgactattt ttgaagctgt atatgtacat
    65701 aaagggatcg ttaatgcagc gaaagtgctt aatctgaccc cctcggcaat cagtcagtct
    65761 attcagaaac tgcgcgttat attccctgac ccattgttta ttcgcaaagg ccagggtgtc
    65821 actcctaccg catttgcgat gcatctacat gagtatatca gtcagggcct tgagtccatt
    65881 cttggcgcgc tggatatcga aggaagctat gataagcaac gaacgataac tattgcgacc
    65941 actccctcgg tcggagccct ggtccttcct gtcatctatc gggcgattaa aactcactat
    66001 ccgcagcttt tactgcgcaa cccgcccatc agcgacgcgg aaaaccaact cagtcagttt
    66061 caaaccgatc tcatcatcga taacatgttt tgcaccaatc gtacggtgca acatcatgtt
    66121 ctgttcaccg acaatatggt gttaatttgc cgcaagggaa atccactact ctctttagaa
    66181 gatgacagag agactatcga caacgctgcg catgtacttc tgttaccgga agggcaaaat
    66241 ttcagcggtc tgcggcagag agttcaagag atgtttccgg accggcaaat caatttcacc
    66301 agctacaaca ttttgacaat cgctgcattg gttgccaaca gtgacatgtt agcgattatt
    66361 cccagccgtt tttataacct gtttagtcgt tgctggccgc tggaaaaact gccttttccg
    66421 tccttaaatg aggagcaaat agatttctct atccactaca acaaattcat cctgcgtgat
    66481 cccatcctgc acggagtaat agatgttatc cgtaatgcat tttgaaaaag tattgttaca
    66541 gccagatcac agcgcagaac gataaaatgg cacaatccaa caacaatttt acattttcgc
    66601 gaccgctttg gctgctttca ggtccgtttc aatgatatac tgccagtcgt taattcaaaa
    66661 atagttgata attacaacaa tctattgaat tgaaacgctt tccttcgtaa ttcgcaactg
    66721 gaacacgcac gctatgagta aacccattgt gatggaacgc ggtgttaaat accgcgatgc
    66781 cgataagatg gcccttatcc cggttaaaaa cgtggcaaca gagcgcgaag ccctgctgcg
    66841 caagccggaa tggatgaaaa tcaagcttcc agcggactct acacgtatcc agggcatcaa
    66901 agccgcaatg cgcaaaaatg gcctgcattc tgtctgcgag gaagcctcct gccctaacct
    66961 ggcggaatgc ttcaaccacg gcacagcaac gtttatgatc ctcggcgcta tttgtacccg
    67021 ccgttgtccg ttctgtgatg ttgcccacgg tcgcccggta gctcctgatg ccaatgaacc
    67081 agtgaaactg gcgcagacca ttgccgatat ggcgctgcgt tatgtggtta tcacctccgt
    67141 tgaccgtgat gacctgcgcg atggcggtgc ccaacacttt gcggattgta ttactgccat
    67201 tcgggaaaaa agcccgcaaa tcaaaattga aactctggtg ccggatttcc gcggtcgaat
    67261 ggatcgtgct ctggatattc tgactgcaac gcccccagat gtgttcaacc ataacctgga
    67321 aaacgtaccg cgtatttacc ggcaggtacg gcctggcgca gattacaact ggtcgctgaa
    67381 gctgctggaa cgctttaaag aagcgcatcc ggaaatcccg accaagtctg gtctgatggt
    67441 ggggctgggt gaaaccaatg aagaaattat tgaggtaatg cgcgacctgc gccgtcatgg
    67501 tgtgacgatg ttaacgctgg ggcaatattt gcagccaagc cgccatcacc tgccggttca
    67561 acgttacgtt agcccggatg agttcgacga aatgaaagcc gaagcgctgg cgatgggctt
    67621 tacccatgct gcatgcggtc cgtttgtccg ctcttcttac cacgccgatt tgcaggcgaa
    67681 aggaatggaa gttaagtaat cctgtaataa ttacttacat ctgtctgata aaaaacccgc
    67741 tttttgaagc gggttttttg tatcgaacaa gatattgagg gagcgtcctg ctcgccacgt
    67801 cactctttat gagagagctt ttcagcctga agatcaacgt ctgcgccttt tttcgccgca
    67861 gcatcgtcat cattcatcgc cttcttgaac cctttaatgg ccgctccaag gtctccgccc
    67921 agcgtacgta acttcttagt cccaaacagc agaacgacca gcgccgcaac taccagcagt
    67981 ttggtaatac taatctcacc catagatacc ttcttgacat ataaccggca acagaacgcg
    68041 ccttaaaaac cagagaatat taacggtcat tcgacgcgcg cacacaatag caatctgtaa
    68101 caaggtgaat caagcgccga gtaaaaaaac atcataataa ttgcggcggc gcaaagcgac
    68161 ggttgtttaa gatgggcagt tgcgcgcgca cctgacgcac acgttcgggc gtcacttccg
    68221 ccataatgag tgcaggcatt tctgacgctg ccgcaatggt gacgccaaag ggatctataa
    68281 tccggctttg cccgatattt ttgttcccgc actcccccgc cgccaccata taacaggtgg
    68341 tatccagcgc acgagcggca agcaacgttg accagtgatg ctctttgagc ggcccacgaa
    68401 cccaggcggc aggaagtacc aggatttcag ctccctgtaa agcctgtgcc agcgccagct
    68461 ctggaaagcg taagtcataa caggtcatca gaccgacctt catcccttcc acctccagta
    68521 acggagctat ttcattacca gcatcaacat ggcgtgattc ctgaatggca aatgcatcat
    68581 agagatgcag tttggcataa cgggcgacga tgtttcctgc ctgaagtgcc accagcatat
    68641 tccatgcgcg ccccggcgtt gaaggaagat gaatcgtcag aattgtcgtc atcatgttac
    68701 gtttactttc tcgccgtaaa cgtccgagga attcgccttc cagcagctgt gctgatttaa
    68761 ccgatagatc tgcatcatga tcatcgcgcg ccagcaatgc ttccggcagg acaaacagcg
    68821 atacgtcgtt ttccgccgcc tgcgccatca acgaggcaca aatctcagcg ttcttttccc
    68881 acacagatgt aacagcaaac tgtcctgcag caaccagcat tgccacctcc tgtcagagtc
    68941 gtaaaattcg ccaacgacga ttattcggcg ttacacttat cactcataca aatcaaatag
    69001 caggattttg cagtgttaca acttctttta gcagttttta ttggcggtgg tacgggaagc
    69061 gtagcgagat ggctgttaag tatgcgattt aacccgctgc atcaggcgat tccgttgggg
    69121 acgctggcag caaacctgat tggggcattc atcataggaa tgggattcgc ctggttcagc
    69181 aggatgacga acattgatcc agtgtggaaa gtattaatca ccaccggatt ttgtggcggt
    69241 ctaacaacct tctcaacatt ttcggcagaa gtggtgtttt tgttacaaga gggccgcttt
    69301 ggctgggcat tactgaacgt tttcgtcaac cttctggggt cttttgccat gaccgcactg
    69361 gcattctggc tgttttcggc ctcaaccgta cactaaagac caaaaaaaac ccgctgatta
    69421 agcgggtttt gaattcttgc tgacgtatct tacagagcga ttacgtttgc agcagaaggg
    69481 cctttggcac cgttagtgat ttcgaactct acgcgctgac cttcagcaag agttttaaaa
    69541 ccattagtct ggattgcaga gaagtgtacg aacacgtctt tgctgccgtc ttccggagta
    69601 atgaaaccga atcctttgga ctcattaaac cacttaacgt tacctttaat cttagacatc
    69661 aaaattacct ttacatgaaa aatagacaca aatgctgtgt cggttatcag tacaccaatt
    69721 gtggtacgct ttgtccagtg caactttact aaaaagtgat aaaagtcgcg ttatttttga
    69781 tgttgcttat tttagttatt gattttaaag aagttactaa aacttcattt gtctcaaaac
    69841 tgaaagcgca tccaggcaaa gtacacattg ccattgttgt aggtacccgg aatgtaggtc
    69901 atctgaaaag tcactgggcc ataacccacg gaggccaatg gcagtagaac cgggagaggg
    69961 atgtaattcc agttatcgcg tgccgttacg ccagcggtga atcccagacc taaatgaaaa
    70021 ttttcatccg ccagcggtcg ccaggtactt tcccatccgt atccggcaat cggttcccat
    70081 ttgttccacg agtccttaaa tgccatggca tacaggccat gccagtttcc tttttcatcc
    70141 caacgcgaca ggccaaaacc gccaccccac ggtcgctcgt tatagcgatc ggttttttct
    70201 ttgtcgtaag cgaaacgtgc atgccaggtg atggcaggaa tatataaatc ataatgttca
    70261 ggctgttgcc aggtttgtgc aatattttct ctaaacgttg tcatcctctc atctgcgtta
    70321 gcaaaaactt taccaacgct gattaactga ataaaaacaa aggaaaagat agcgacatat
    70381 ttactcacgt tcatttgtga ccataaaaca tttatcaaaa atctactact agcatagcaa
    70441 agctacaatt aacataacct taatagaccc aacataaaga ataatctgaa tagctgctct
    70501 atttatttcc gcttgttcaa gttaaatatt tccgcgcatc gattaaagat gatgcgtcgt
    70561 tttctgcgat gggaatagtc aaaaaagaaa aaccaagtct tttttgatga caaaatgcaa
    70621 tcaaggaaaa attattttat ttttttaacg agatacaaca atcatcttaa cgaagtatat
    70681 aatatatatc gtcaacccat gctcatatta gattattttt tgagcgcaca gccgcagcta
    70741 acaccattgc aattaacaaa tttgcatcaa tccaccatca atttacacac attattatgt
    70801 gataattgcc aaccgctaaa tatgcgtttt gttatctatg tataaaaaca gcaacttcaa
    70861 tgtcttaatg gcagtttttc ttgattttaa tcagcattca tcgccaattt attgggcata
    70921 ttttttcctt aagctttagg aagtttttat tatttacttt gggcctggag acaggaaaaa
    70981 ttatgctgac attcattgaa ctccttattg gggttgtggt tattgtgggt gtggctcgct
    71041 acatcattaa agggtattcc gccactggtg tgttatttgt cggtggcctg ttattgctga
    71101 ttatcagtgc cattatgggg cacaaagtgt taccgtccag ccaggcttca acaggctaca
    71161 gcgccacgga tatcgttgaa tacgttaaaa tattactaat gagccgcggc ggcgacctcg
    71221 gcatgatgat tatgatgctg tgtggctttg ccgcttacat gacccatatc ggcgcgaatg
    71281 atatggtggt caagctggcg tcaaaaccat tgcagtatat taactcccct tacctgctga
    71341 tgattgccgc ctattttgtt gcttgtctga tgtcactggc cgtctcttcc gcaaccggtc
    71401 tgggtgtttt gctgatggca accctgtttc cggtgatggt aaacgttggt atcagtcgtg
    71461 gcgcagcagc tgccatttgt gcctccccgg cggcgattat tctcgcaccg acttcagggg
    71521 atgtggtgct ggcggcgcag gcttccgaaa tgtcgctgat tgacttcgcc ttcaaaacga
    71581 cgctgcctat ctcaattgct gcaattatcg gcatggcgat cgcccacttc ttctggcaac
    71641 gttatctgga taaaaaagag cacatctctc atgaaatgtt agatgtcagt gaaatcacca
    71701 ccactgcccc cgcgttttat gccattttgc cgttcacgcc gatcatcggt gtactgattt
    71761 ttgacggtaa atggggtccg caattacaca tcatcactat tctggtgatt tgtatgctga
    71821 ttgcctccat tctggagttc ctccgcagct ttaataccca gaaagttttc tctggtctgg
    71881 aagtggctta tcgcgggatg gcagatgcgt ttgctaacgt ggtgatgctg ctggttgccg
    71941 ctggggtatt cgctcagggg cttagcacca tcggctttat tcaaagtctg atttctatcg
    72001 ctacctcgtt tggttcggcg agtatcatcc tgatgctggt attggtgatt ctgaccatgc
    72061 tggcggcagt cacgaccggt tcaggcaatg cgccgtttta tgcgtttgtt gagatgatcc
    72121 cgaaactggc gcactcttcc ggcattaacc cggcgtattt gactatcccg atgctgcagg
    72181 cgtcaaacct gggtcgtacc ctatcacccg tttctggcgt agtcgttgcg gttgccggga
    72241 tggcgaaaat ctcaccattt gaagtcgtaa aacgcacctc ggtgccggtg cttgttgggc
    72301 tagtgattgt tatcgttgct acagagctga tggtgccagg aacggcagca gcggtcacag
    72361 gcaagtaaat agtaatgccg gaggcaagtt tcctccggct ttatcatgtc acccactgtg
    72421 gtatatgcgt tgtggtctgc caactttgcc gtgaacaatt tcagcaataa tcagatggcg
    72481 gctggcgcaa tattcaagat aacgcctggc agtggtgcgg ctgatggtca gtgcctgcgc
    72541 caccgtttct gccgtatgtt gcacaccagg ctctttaaac agttttcgca ccgcgtttag
    72601 cgtcaagggg tcaatgccgg tcggcagttc gtctttgggt tcaccgcgag cataggcatt
    72661 aaacatctca tcaatttgct tctggctggc gctatcaata ctttccagca tatgtttacg
    72721 ctggcggaaa cgggttagcg tttgccccag tgtattgatt cacttgaagt acgaaaaaac
    72781 cgggagaaca ttggattatt cgggatctga tgggattaga tttggtgggg cttgcaagcc
    72841 tgtagtacaa attttaggcg ttaaccaatg aaacgcgaaa gatagtaaaa aattgctttt
    72901 gtttcattga aaaaacgaaa aacaaaaaca ctataaatca tttcaatcat agcttcaaaa
    72961 aacgttcaaa accgataaca accaggctgt caccaaatga ctcatatcac aaatcagctc
    73021 atgccgttta ggtatgttac atgtgtgatt atgtgaggtg aagtatgttt tagctggctc
    73081 atggttgtta tacggctttt ttcgcatctt gtggttccaa caaaggagct acaggctctt
    73141 cctgtttatg aacaccatac attgacaaaa tctcttggcg agtttctgga ggtaatttat
    73201 gcagcgccaa agctgtttgt agtgtttggc gatcaatacc cagcgttgcc aatagggtgt
    73261 caacggagat ggaggggcgt tcttgcatta atccggaaag cccatattta gccatgttta
    73321 gctctatctc tcggacttca ttttctccta gtttattgat taacagggag aacattttca
    73381 atgtatcagc attcatttct tcgatagcaa ttttttgcat agccggtatt gccgggatat
    73441 attcaacccc aggcccgcga cgcccttctt gtgaacgctt cggccatcct tctttctttg
    73501 ctcgtttatg caccccctgt attgtccctg gcattccatc cagttcagta atttgtttgg
    73561 gtgtcatcca ctggtttatc tcatttgcgc ttttcataaa ctaagtttac cagatttatc
    73621 tatataaaaa tcaatggatt acaaaataaa ccacattaac tgatataaac cagttttgtt
    73681 tatttggtaa tgtataaaac agataatcaa gacgaattat ttgttttggt aaccctttat
    73741 catacgcaat atggttggtg tgcgaaagat gagtaacgat tggcatcggg ccgacgtgat
    73801 tgcggccctc aaaaaaacag gaacccctct ggcaaaattg tccagacgtt caggttattc
    73861 aaccgggacg cttgcaaatg cattagtcag gccatggcct aaaggtgaac gcattattgc
    73921 tgacgcctta aacatgaaac ctgaggaaat ttggcctagt agatatctaa gggatcacaa
    73981 tgagagagtg gatatcagca attgaatgca tgggtttatc tggtttacct ggttcattaa
    74041 tgggggttca caaaagagct cagagagaag gatggctcaa gagaagaaga gaaggaatta
    74101 gaggggttgc ttacgaatac catatatctt ctcttcctaa cattgctcga gctgaagttt
    74161 tgctccaaca aggaaaattt aaaaccagtc agggatgttt tgaaatcgcc cgccccacgc
    74221 tggaagctca tgattatgat cgtgaggcac tatggagcaa atgggataaa gccagcgatt
    74281 cccagcgcag cctggctgaa aaatggttgc cctcaattca ggccacagac gaaatgctga
    74341 accaggggat ttcaacgaaa acggcttttg cgaccgttgc agggcattac caggtcagcg
    74401 catccacttt gcgggacaag tattaccagg tacagaagtt tgcgaagcct gactgggcgg
    74461 ctgcacttgt tgatggacgt ggagcatccc gtcgcaatgt tcacaaaagt gaatttgacg
    74521 aggatgcctg gcagtttctg attgcagatt atctgcgacc ggaaaaaccc gccttccgca
    74581 aatgttatga gcgtctggaa ctggcagtcc gcgagcatgg ctggagtatt ccctcccgtg
    74641 ccacggcctt tcgccggatt cagcaactgg acgaggcaat ggttgttgcc tgtcgtgaag
    74701 gtgaacatgc actgatgcat ctgataccgg cacagcagcg aactgtggaa cacctggacg
    74761 ccatgcagtg gatcaacggc gacggttatc tgcataacgt ctttgtacgc tggtttaacg
    74821 gtgatgtgat ccgtccgaaa acatggttct ggcaggatgt gaaaacccga aaaattctgg
    74881 gctggcgctg cgatgtgagc gagaacattg attcaattcg cctctcgttc atggatgttg
    74941 tgactcgcta cagtatcccg gaggattttc acatcaccat tgataacacc cgtggtgctg
    75001 cgaataaatg gctgacggga ggcgcgccca atcgctaccg ctttaaggta aaagaggacg
    75061 atccaaaagg actgttttta ctgatgggtg cgaaaatgca ctggacaagc gttgttgccg
    75121 gtaaaggctg gggccaggca aaacctgttg aacgtgcttt cggtgttggt gggcttgagg
    75181 aatacgttga taagcatccg gcactggctg gcgcatatac ggggccaaat ccgcaggcaa
    75241 aacctgataa ctatggcgac cacgctgttg atgcagagct gtttctgaaa acctttgccg
    75301 aaggtgtggc gatgttcaat gccagaacag gccgtgaaac agaaatgtgc gggggcaaac
    75361 tctcgtttga tgatgttttc gagcgtgaat acgccagaac gattgtgcgt aagccaaccg
    75421 aagaacaaaa acggatgctg ttactgcctg ccgaggcggt gaacgtttca cgcaaaggcg
    75481 agtttacgct taaagttggc ggctccctta aaggcgcgaa aaacgtttat tacaacatgg
    75541 cattaatgaa tgccggagtg aaaaaagttg tggtcagatt tgatccacag cagttacaca
    75601 gcacggttta ttgag
//
LOCUS       DAOFMX010000005        72888 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.005, whole genome shotgun sequence.
ACCESSION   DAOFMX010000005 DAOFMX010000000
VERSION     DAOFMX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.005"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="QBA20_000445"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="QBA20_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDQ6291106.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="QBA20_000446"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="QBA20_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDQ6291107.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="QBA20_000447"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="QBA20_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDQ6291108.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="QBA20_000448"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="QBA20_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDQ6291109.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="QBA20_000449"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="QBA20_000449"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDQ6291110.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="QBA20_000450"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="QBA20_000450"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDQ6291111.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="QBA20_000451"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="QBA20_000451"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDQ6291112.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="QBA20_000452"
     CDS             complement(4598..5320)
                     /locus_tag="QBA20_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ6291113.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMAGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="QBA20_000453"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="QBA20_000453"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDQ6291114.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="QBA20_000454"
     CDS             6520..6738
                     /locus_tag="QBA20_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDQ6291115.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="QBA20_000455"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="QBA20_000455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDQ6291116.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="QBA20_000456"
     CDS             complement(7472..7672)
                     /locus_tag="QBA20_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDQ6291117.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="QBA20_000457"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="QBA20_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="QBA20_000458"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="QBA20_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDQ6291118.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="QBA20_000459"
     CDS             10101..12014
                     /locus_tag="QBA20_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6291119.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="QBA20_000460"
     CDS             12014..13036
                     /locus_tag="QBA20_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDQ6291120.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLANEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="QBA20_000461"
     CDS             13030..13248
                     /locus_tag="QBA20_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDQ6291121.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="QBA20_000462"
     CDS             13302..14171
                     /locus_tag="QBA20_000462"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDQ6291122.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSRETVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="QBA20_000463"
     CDS             complement(14226..14630)
                     /locus_tag="QBA20_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDQ6291123.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="QBA20_000464"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="QBA20_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDQ6291124.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="QBA20_000465"
     CDS             15615..17705
                     /locus_tag="QBA20_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDQ6291125.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLRLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="QBA20_000466"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="QBA20_000466"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDQ6291126.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="QBA20_000467"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="QBA20_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDQ6291127.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="QBA20_000468"
     CDS             complement(19673..20275)
                     /locus_tag="QBA20_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDQ6291128.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="QBA20_000469"
     CDS             complement(20265..20432)
                     /locus_tag="QBA20_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDQ6291129.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21109)
                     /gene="ppiA"
                     /locus_tag="QBA20_000470"
                     /gene_synonym="rotA"
     CDS             complement(20537..21109)
                     /gene="ppiA"
                     /locus_tag="QBA20_000470"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDQ6291130.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21380..22561
                     /gene="tsgA"
                     /locus_tag="QBA20_000471"
     CDS             21380..22561
                     /gene="tsgA"
                     /locus_tag="QBA20_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDQ6291131.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22823..25366
                     /gene="nirB"
                     /locus_tag="QBA20_000472"
     CDS             22823..25366
                     /gene="nirB"
                     /locus_tag="QBA20_000472"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDQ6291132.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25363..25689
                     /gene="nirD"
                     /locus_tag="QBA20_000473"
     CDS             25363..25689
                     /gene="nirD"
                     /locus_tag="QBA20_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDQ6291133.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25815..26621
                     /gene="nirC"
                     /locus_tag="QBA20_000474"
     CDS             25815..26621
                     /gene="nirC"
                     /locus_tag="QBA20_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDQ6291134.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26640..28013
                     /gene="cobA"
                     /locus_tag="QBA20_000475"
     CDS             26640..28013
                     /gene="cobA"
                     /locus_tag="QBA20_000475"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDQ6291135.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28260..28427
                     /locus_tag="QBA20_000476"
     CDS             28260..28427
                     /locus_tag="QBA20_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDQ6291136.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28722..30059
                     /gene="frlA"
                     /locus_tag="QBA20_000477"
                     /pseudo
     CDS             28722..30059
                     /gene="frlA"
                     /locus_tag="QBA20_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30080..31102
                     /gene="frlB"
                     /locus_tag="QBA20_000478"
     CDS             30080..31102
                     /gene="frlB"
                     /locus_tag="QBA20_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HDQ6291137.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31152..31982
                     /gene="frlC"
                     /locus_tag="QBA20_000479"
     CDS             31152..31982
                     /gene="frlC"
                     /locus_tag="QBA20_000479"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HDQ6291138.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31979..32764
                     /gene="frlD"
                     /locus_tag="QBA20_000480"
     CDS             31979..32764
                     /gene="frlD"
                     /locus_tag="QBA20_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDQ6291139.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32864..33595
                     /gene="phoB"
                     /locus_tag="QBA20_000481"
     CDS             32864..33595
                     /gene="phoB"
                     /locus_tag="QBA20_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HDQ6291140.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33755..34840)
                     /locus_tag="QBA20_000482"
     CDS             complement(33755..34840)
                     /locus_tag="QBA20_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDQ6291141.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34852..36156)
                     /locus_tag="QBA20_000483"
     CDS             complement(34852..36156)
                     /locus_tag="QBA20_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDQ6291142.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36168..36521)
                     /locus_tag="QBA20_000484"
     CDS             complement(36168..36521)
                     /locus_tag="QBA20_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDQ6291143.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36532..37410)
                     /locus_tag="QBA20_000485"
     CDS             complement(36532..37410)
                     /locus_tag="QBA20_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDQ6291144.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37407..38633)
                     /locus_tag="QBA20_000486"
     CDS             complement(37407..38633)
                     /locus_tag="QBA20_000486"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDQ6291145.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38634..39796)
                     /locus_tag="QBA20_000487"
                     /pseudo
     CDS             complement(38634..39796)
                     /locus_tag="QBA20_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            complement(39880..40242)
                     /locus_tag="QBA20_000488"
     CDS             complement(39880..40242)
                     /locus_tag="QBA20_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDQ6291146.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40259..41164)
                     /locus_tag="QBA20_000489"
     CDS             complement(40259..41164)
                     /locus_tag="QBA20_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291147.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41434..42438)
                     /gene="trpS"
                     /locus_tag="QBA20_000490"
     CDS             complement(41434..42438)
                     /gene="trpS"
                     /locus_tag="QBA20_000490"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDQ6291148.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42431..43189)
                     /gene="gph"
                     /locus_tag="QBA20_000491"
     CDS             complement(42431..43189)
                     /gene="gph"
                     /locus_tag="QBA20_000491"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDQ6291149.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43182..43859)
                     /gene="rpe"
                     /locus_tag="QBA20_000492"
     CDS             complement(43182..43859)
                     /gene="rpe"
                     /locus_tag="QBA20_000492"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDQ6291150.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43877..44713)
                     /gene="dam"
                     /locus_tag="QBA20_000493"
     CDS             complement(43877..44713)
                     /gene="dam"
                     /locus_tag="QBA20_000493"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDQ6291151.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44820..46106)
                     /gene="damX"
                     /locus_tag="QBA20_000494"
     CDS             complement(44820..46106)
                     /gene="damX"
                     /locus_tag="QBA20_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDQ6291152.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46198..47286)
                     /gene="aroB"
                     /locus_tag="QBA20_000495"
     CDS             complement(46198..47286)
                     /gene="aroB"
                     /locus_tag="QBA20_000495"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDQ6291153.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47343..47864)
                     /gene="aroK"
                     /locus_tag="QBA20_000496"
     CDS             complement(47343..47864)
                     /gene="aroK"
                     /locus_tag="QBA20_000496"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDQ6291154.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48265..49503)
                     /gene="hofQ"
                     /locus_tag="QBA20_000497"
     CDS             complement(48265..49503)
                     /gene="hofQ"
                     /locus_tag="QBA20_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDQ6291155.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QEREIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49415..49819)
                     /gene="hofP"
                     /locus_tag="QBA20_000498"
     CDS             complement(49415..49819)
                     /gene="hofP"
                     /locus_tag="QBA20_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDQ6291156.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49809..50249)
                     /gene="hofO"
                     /locus_tag="QBA20_000499"
     CDS             complement(49809..50249)
                     /gene="hofO"
                     /locus_tag="QBA20_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDQ6291157.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50233..50772)
                     /gene="hofN"
                     /locus_tag="QBA20_000500"
     CDS             complement(50233..50772)
                     /gene="hofN"
                     /locus_tag="QBA20_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDQ6291158.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50772..51551)
                     /gene="hofM"
                     /locus_tag="QBA20_000501"
     CDS             complement(50772..51551)
                     /gene="hofM"
                     /locus_tag="QBA20_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDQ6291159.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51671..54223
                     /gene="mrcA"
                     /locus_tag="QBA20_000502"
     CDS             51671..54223
                     /gene="mrcA"
                     /locus_tag="QBA20_000502"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDQ6291160.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54388..54948)
                     /gene="nudE"
                     /locus_tag="QBA20_000503"
     CDS             complement(54388..54948)
                     /gene="nudE"
                     /locus_tag="QBA20_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDQ6291161.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55268..57403
                     /gene="igaA"
                     /locus_tag="QBA20_000504"
     CDS             55268..57403
                     /gene="igaA"
                     /locus_tag="QBA20_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDQ6291162.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     YRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57468..58136
                     /gene="yrfG"
                     /locus_tag="QBA20_000505"
     CDS             57468..58136
                     /gene="yrfG"
                     /locus_tag="QBA20_000505"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDQ6291163.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58147..58548
                     /gene="hslR"
                     /locus_tag="QBA20_000506"
     CDS             58147..58548
                     /gene="hslR"
                     /locus_tag="QBA20_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDQ6291164.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58573..59442
                     /gene="hslO"
                     /locus_tag="QBA20_000507"
     CDS             58573..59442
                     /gene="hslO"
                     /locus_tag="QBA20_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDQ6291165.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59586..61310)
                     /locus_tag="QBA20_000508"
     CDS             complement(59586..61310)
                     /locus_tag="QBA20_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDQ6291166.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61689..63311
                     /gene="pckA"
                     /locus_tag="QBA20_000509"
     CDS             61689..63311
                     /gene="pckA"
                     /locus_tag="QBA20_000509"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDQ6291167.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63387..64739)
                     /gene="envZ"
                     /locus_tag="QBA20_000510"
     CDS             complement(63387..64739)
                     /gene="envZ"
                     /locus_tag="QBA20_000510"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDQ6291168.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLSTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64736..65455)
                     /gene="ompR"
                     /locus_tag="QBA20_000511"
     CDS             complement(64736..65455)
                     /gene="ompR"
                     /locus_tag="QBA20_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDQ6291169.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65683..66159
                     /gene="greB"
                     /locus_tag="QBA20_000512"
     CDS             65683..66159
                     /gene="greB"
                     /locus_tag="QBA20_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDQ6291170.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66256..68577
                     /locus_tag="QBA20_000513"
     CDS             66256..68577
                     /locus_tag="QBA20_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDQ6291171.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69015..69242
                     /gene="feoA"
                     /locus_tag="QBA20_000514"
     CDS             69015..69242
                     /gene="feoA"
                     /locus_tag="QBA20_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDQ6291172.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69259..71580
                     /gene="feoB"
                     /locus_tag="QBA20_000515"
     CDS             69259..71580
                     /gene="feoB"
                     /locus_tag="QBA20_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDQ6291173.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71580..71816
                     /gene="feoC"
                     /locus_tag="QBA20_000516"
     CDS             71580..71816
                     /gene="feoC"
                     /locus_tag="QBA20_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDQ6291174.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72019..>72888
                     /gene="rpnA"
                     /locus_tag="QBA20_000517"
     CDS             72019..>72888
                     /gene="rpnA"
                     /locus_tag="QBA20_000517"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HDQ6291175.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAA"
BASE COUNT        17560 a        19753 c        18435 g        17140 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca aaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccaaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgccatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt aatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaatga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aaacagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattgcacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactac gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacggatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc gaaaacagca gccatcgccg ccagggtcga tttgaacatg agatttcctt
    21121 aacaaacaga gcagaaaaaa agcaagtgaa gagattctaa atagccttca agaacaaggc
    21181 catcccttta cctaatttta cgtatctgaa atatatgtaa cctgacgatc actttttatt
    21241 ccgtgatcaa aatcacctct taaaatgcaa tttagcaacc gattgcaata aaacatttaa
    21301 acagatcata aaatcaccta aaatcgcccg ctcgcggcat atcccgctat gccattttat
    21361 ttttgaacgc aggccaatca tgactaacag caatcgcatc aagctcacat ggattagctt
    21421 tctctcctac gcactgactg gtgcgttggt tattgtcacc gggatggtga tgggaaatat
    21481 cgccgattat ttcaatctgc ctgtttccag tatgagtaac accttcacct tcctcaacgc
    21541 cggcatttta atctctatct tcctcaacgc ctggctgatg gaaatcgtcc cgttgaaaac
    21601 gcagttacgt tttggctttc tcctgatggt gctggcggtt gccggtttga tgttcagcca
    21661 cagcctggcg ctgttctcga cggcgatgtt cattctcggg gtggtcagcg gcattactat
    21721 gtcgattggt acattcctga taacacaaat gtatgaaggg cgtcagcgcg gttcccgcct
    21781 gttatttact gactccttct tcagtatggc tgggatgatt ttcccaatga tcgccgcgtt
    21841 tctactggcg cgcagcattg agtggtactg ggtttatgcc tgcatcgggc tggtgtatgt
    21901 cgctattttt attctgacct tcggctgtga gttcccggca ctgggcaaac gtgcaccaaa
    21961 aacggatgct ccggtagaga aagaaaagtg ggggatcggc gtactgtttc tctctgttgc
    22021 ggcgctgtgc tacatcctcg gtcagttagg ttttatctcc tgggtgcctg agtatgccaa
    22081 aggcctgggc atgagcctga acgacgcggg cacgctggtg agtaacttct ggatgtcata
    22141 catggtcggc atgtgggcgt tcagctttat tcttcgcttc tttgatttgc aacgcattct
    22201 gaccgtactg gctggtctgg ctgcgattct gatgtacgtc tttaacaccg gaacaccagt
    22261 acatatggcg tggtcaattc tcgctctggg cttcttctcc agcgcgatct ataccaccat
    22321 catcaccctg ggttcacagc agaccaaagt accgtcgcca aaactggtta actttgtcct
    22381 gacctgcgga accatcggta ctatgttgac ctttgtggtt accggcccga ttgttgaaca
    22441 tagcggtccg caggcggcac tgcttacagc aaacggtctg tacgctgtcg tctttgtgat
    22501 gtgcttcctg ttaggtttcg tcagccgtca ccgtcagcat aacaccctga cctctcatta
    22561 attgctaatg ccggacggca ctatcgtcgt ccggcctttt cctctcttcc cccgctacgt
    22621 gcatctattt ctataaaccc gttcattttg tccatttttt gcacaaacat gaaatgtcag
    22681 acaattccgt gacttaagaa aatttataca aatcagcaat atacccctta aggagtatat
    22741 aaaggtgaat ttgatttaca tcaataagcg gggttgctga atcgttaagg taggcggtaa
    22801 tagaaaagaa atcgaggcaa aaatgagcaa agtcagactc gcaattatcg gtaacggtat
    22861 ggtcggccat cgctttatcg aagatcttct tgataaatct gatgcggcca actttgatat
    22921 taccgttttc tgtgaagaac cgcgcatcgc ttatgaccgc gtacacctct cgtcttactt
    22981 ctctcaccac accgccgaag agctgtcgct ggtgcgcgaa ggcttctacg agaaacacgg
    23041 catcaaagtt ctggtcggcg aacgcgctat caccatcaac cgtcaggaga aggtgattca
    23101 ctccagcgcc ggacgtaccg ttttttatga caagctgatc atggcaaccg gttcctaccc
    23161 gtggatccca ccaatcaaag gttctgatac tcaggactgc tttgtctatc gcaccattga
    23221 agacctcaac gccattgaat cctgcgcccg tcgcagcaaa cgcggtgccg ttgttggtgg
    23281 cggcctgtta ggtctggaag ccgctggcgc gctgaaaaac ttaggtattg aaacccacgt
    23341 tatcgaattt gcccctatgc tgatggcgga acagcttgat cagatgggtg gcgagcagct
    23401 gcgtcgcaaa atcgaaagta tgggcgtgcg cgttcacacc agcaaaaaca cccttgagat
    23461 tgtgcaggaa ggtgtcgaag cgcgtaaaac catgcgtttt gccgacggca gcgaactgga
    23521 agtcgacttt atcgtcttct ctaccggtat ccgtccgcgc gataagctgg caacccagtg
    23581 tggtctggac gttgctccgc gtggggggat tgtcattaat gattcctgcc agacttccga
    23641 tccggatatc tacgccatcg gtgaatgcgc aagctggaac aaccgtgtat ttggtctggt
    23701 agcaccaggc tacaaaatgg cgcaggtcgc cgttgaccat attctgggta gcgaaaacgc
    23761 ctttgaaggt gctgacctta gcgccaagct gaaactgctg ggcgtagacg taggtggtat
    23821 tggtgatgcg cacggtcgca cgcctggcgc acgtagctat gtttacctcg acgaaagtaa
    23881 agagatctac aaacgcctga ttgtcagcga agacaacaaa accctgctcg gtgcggtact
    23941 ggtgggcgat accagcgact acggcaacct gctgcaactg gtgctgaacg ctatcgagct
    24001 gccggaaaac ccggattctc tgattctgcc ggcgcactcg ggtagcggta agccgtctat
    24061 cggtgttgat aaactgccgg acagcgcgca aatctgctcc tgcttcgacg tcactaaagg
    24121 tgatctgatt gctgccatca acaaaggctg ccacacagtt gcggcgctga aagctgaaac
    24181 caaagcgggt actggctgcg gtggctgtat cccgctggtc actcaggtac tgaacgcgga
    24241 actggcgaaa cagggcatcg aagttaacaa caacctgtgc gaacactttg cttattcgcg
    24301 tcaggaactg ttccatttga tccgcgttga aggcattaaa accttcgaag aactgctggc
    24361 gaaacacggc aaaggctacg gttgtgaagt ttgtaaacca accgtcggtt cgctgctggc
    24421 ctcctgctgg aacgaataca ttctgaagcc ggaacatact ccgctgcagg attctaacga
    24481 caacttcctc gctaacatcc agaaagacgg cacctactcg gtgatcccgc gttctccggg
    24541 cggtgaaatc accccggaag ggctgatggc agtgggtcgt atcgcgcgtg aatttaatct
    24601 ctacaccaaa atcaccggct cccagcgcct ggcgatgttt ggcgcacaga aagacgatct
    24661 gccggaaatc tggcgtcagc tgattgaagc tggcttcgaa accggtcatg cctatgcgaa
    24721 agcactgcgt atggcgaaaa cctgcgtggg tagcacctgg tgccgctacg gtgttggcga
    24781 tagcgtcggc ctcggcgtgg aactggaaaa ccgctacaaa ggcatccgta cgccgcacaa
    24841 aatgaagttc ggtgtctccg gctgtacccg tgaatgttca gaagctcagg gtaaagacgt
    24901 gggtattatc gccactgaaa aaggctggaa cctgtatgtt tgcggtaacg gcggcatgaa
    24961 accgcgtcat gcggatctgc tggcagcgga tatcgatcgc gaaacgctga tcaaatatct
    25021 cgaccgcttc atgatgttct acatccgtac cgccgacaaa ctgacgcgta ccgcgccgtg
    25081 gttagaaaac ctcgaaggcg gcatcgatta cctgaaagcg gtgatcattg acgacaaact
    25141 ggggctgaac gctcatctgg aagaagagat ggcgcgcctg cgtgaagcgg tagtgtgtga
    25201 gtggactgaa acggtcaata caccgtccgc gcagactcgc ttcaaacact tcatcaacag
    25261 cgacaagcgt gacccgaacg tgcagatggt gccagagcgc gaacagcacc gtccggcaac
    25321 gccgtatgaa cgtatcccgg taactctggt ggaggacaac gcatgagcca gtggaaagac
    25381 atctgcaaaa tcgatgacat cctgcctgaa accggcgtct gcgcactgtt aggtgacaag
    25441 caggtagcga ttttccgccc gtatcacagc gaccaggtgt ttgcgatcag caacattgat
    25501 ccgttcttcg agtccagcgt gctatcacgc gggctgattg cggaacacca gggcgagctg
    25561 tgggtcgcca gcccgctgaa aaaacagcgt tttcgcttaa gtgatggctt gtgcatggaa
    25621 gacgaacagt tttccgtcaa acattacgaa gcacgagtga aagacggcgt ggtgcagctg
    25681 cgcggttaat gttttaacgg gaggcgcaat gcctcccctt tttgcatggt cctgtaatga
    25741 ccttcggtat attgcaggac attttttaaa ctttttgttt tattttttgt ttttattttt
    25801 taaaggataa tcaaatgttt acagacacta ttaataagtg tgcggctaat gctgcgcgca
    25861 ttgcacgcct gtcggcaaat aacccgctcg gcttttgggt cagctccgcc atggcgggcg
    25921 catatgtggg tcttgggatc atcctgattt tcacgctcgg taatttgctc gacccgtccg
    25981 tacgtccgct ggtgatgggc gcgacctttg gtatcgcctt aacgctggtg attatcgccg
    26041 gttctgaact gttcaccgga cacaccatgt tcctcacctt tggggtaaaa gcgggcagca
    26101 tcagccacgg gcaaatgtgg gcaatcctgc cgcaaacctg gctgggtaac ctggtcggtt
    26161 ccgtcttcgt tgccatgctc tatagctggg gcggcggtag cctgctgcca gtagatacca
    26221 gcatcgttca ctccgtcgcg ctggctaaaa ccactgcacc ggcaatggta ctgttcttca
    26281 aaggtgcatt gtgtaactgg ctggtttgcc tggcaatctg gatggcgctg cgcactgaag
    26341 gggcggcgaa atttatcgct atctggtggt gtctgctggc atttatcgcg tccggctacg
    26401 agcactctat cgctaacatg acgctgttcg cgctctcctg gttcggcaac cacagcgaag
    26461 cctacacgct ggcgggtatt ggtcataacc tgctgtgggt gacgctgggt aatactttat
    26521 caggtgccgt attcatggga ttgggttatt ggtatgctac gccgaaagcg aatcgtccgg
    26581 ttgcggacaa atttaatcaa actgaaacag ctgccggtta attactaagg ggtttttacg
    26641 tggatcattt gcctatattt tgccaattac gcgatcgcga ctgtctgatt gtcggcggtg
    26701 gtgatgtcgc ggaacgcaaa gcaaggttgc tgttagacgc aggcgctcgc ttaacggtga
    26761 atgcattagc gtttattcca cagttcaccg catgggcaga tgcaggcatg ttaaccctcg
    26821 tcgaagggcc atttgatgaa agccttctcg acacctgctg gctggcgatt gcagcgacgg
    26881 atgatgacac gcttaaccag cgcgtcagcg aagccgctga agctcgtcgc atcttctgta
    26941 acgtagttga tgcgccgaaa gccgccagct ttattatgcc gtcgattatt gaccgctcac
    27001 cgctaatggt agcggtctcc tctggcggca cctctccggt tctggcgcgc ctgttgcgcg
    27061 aaaaactcga atcactgctg ccgttgcatc tgggccaggt ggcgaaatat gccgggcaat
    27121 tacgcgggcg agtgaaacaa cagttcgcca cgatgagtga gcgtcgccgt ttctgggaga
    27181 aattgttcgt taacgaccgc ctggcgcagt cgctggcaaa caacgatcag aaagccatta
    27241 ctgaaacgac cgaacagtta atcaacgaac cgctcgacca tcgcggtgaa gtggtgctgg
    27301 ttggtgcagg tccgggcgat gccgggctgc tgacgctgaa aggactgcaa caaattcagc
    27361 aggcagatgt ggtggtctac gaccgtctgg tttctgacga tattatgaat ctggtacgcc
    27421 gcgatgctga tcgcgttttc gtcggcaaac gcgcgggata ccactgtgta ccgcaggaag
    27481 agattaacca gatcctgctg cgggaagcgc aaaaaggcaa acgcgtggtg cggctgaaag
    27541 gtggcgatcc gtttattttt ggccgtggtg gcgaagagct ggaaacactg tgcaacgcgg
    27601 gtattccgtt ctcggtggtt ccgggtatta ccgcagcttc tggttgctct gcctattcgg
    27661 gtattccact cacgcatcgc gattatgccc agagcgtacg cttaattacc ggacacttaa
    27721 aaaccggtgg cgagctggac tgggaaaacc tggcggcaga aaaacagacg ctggtgttct
    27781 atatggggtt gaatcaggcc gcgactattc agcaaaagct gattgaacac ggtatgcctg
    27841 gcgaaatgcc ggtggcaatt gtcgaaaacg gaacggcagt cacgcagcgc gtgattgacg
    27901 gtacgctcac gcaactcggt gaacttgctc agcaaatgaa cagtccatcg ctaattatta
    27961 ttggtcgggt tgttggcctg cgcgataaac tgaactggtt ctccaatcat taatttaacc
    28021 cgggccagag aattctggcc ttcttaatga ctctttttat atattcagca aataaaacat
    28081 aaccccttaa taaatattat ctgctaatgg gttatattca ccgttacagc gacttacaaa
    28141 attaagtcat tcgctcactg acgccaatta aatattcctg tatcgttctt aacgagcagt
    28201 ctgcttatat tcatataatc aatgaatatt aattaatata taatacatag ggaatgtaaa
    28261 tgaacaaatt tattaaagtt gcactggtag gtgcagtact ggctacgtta actgcatgta
    28321 ctggtcatat tgaaaaccgt gataagaact gctcttacga ctacctgctg cacccggcaa
    28381 tttctatttc taaaatcatt ggcggttgcg gtcctactgc acagtaagtc tctgacagaa
    28441 atcggctaac accggtttaa ttgcccgacg ccctctggat tttccgaggg cgtatttgtt
    28501 tctatgtcct tcttgccccc gcccgtaaaa atattttcac tttaaattca attttataac
    28561 tacatccatt agccacagcg cgatcctgtg cgaaattttg tgatcttcct ccacattaca
    28621 caacataata tgttgttata ttcatcatgc attgtcatgt taccttttaa atgactgcaa
    28681 actctcccct acaacgtatt ggacaagaaa aaggtatcgc tatgggaagc caggaactcc
    28741 aacgcaagct cggattttgg gccgttcttg caatcgccgt cgggacaacc gtcggctccg
    28801 gtatttttgt atctgtgggt gaagtggcaa aagcagcggg cacgccgtgg cttacggtgc
    28861 tcgcgtttgt cattggcggg ttaattgtga tcccgcaaat gtgcgtctat gcggaactat
    28921 ccaccgctta tccggaaaat ggcgcagatt atgtttatct gaaaaatgcc ggaagccgac
    28981 cgctggcttt cctctccggc tgggccagct tctgggccaa cgatgcgccg tcattgtcga
    29041 ttatggcgct ggcgattgtc agcaatcttg gctttttaac gcctatcgat ccgttgctcg
    29101 gtaaatttat cgccgccgga ttaattatcg cctttatgtt gctacacctg cgctccgttg
    29161 aaggcggcgc agcgtttcag acgctaatta ccatcgccaa aattatcccg ttcactatcg
    29221 tcattggcct tgggatcttc tggtttaaag cagagaattt tgccgccctt accaccactg
    29281 cgattggcgc aacgggcagc tttatggcgc tgctggcggg gatctctgcc accagttggt
    29341 cgtataccgg catggcctct atctgttata tgaccggcga aattaaaaac cccggaaaaa
    29401 ccatgccacg agcgctgatt ggttcctgtc tgctggttct ggtgctctac accctgctgg
    29461 cgctggtgat ttccggcctg atgcccttcg acaaactcgc caattctgaa acgccgattt
    29521 ccgacgccct gacctagatc cccgcactcg gcagcaccgc tgggctcttt gttgccatca
    29581 cggcgatgat cgtcattctt ggttcgcttt ccagctgcgt gatgtaccag ccgcggctgg
    29641 aatacgcgat ggcgaaagac aacctgttct ttaaatgctt cggccatgtg catccgaaat
    29701 acaacacgcc ggatgtctcc atcatcctgc aaggggcgct ggggatcttc ttcatcttcg
    29761 tttccgatct caccagcctg ctgggttatt tcaccctggt gatgtgtttc aaaaataccc
    29821 tcaccttcgg ctccatcatc tggtgtcgta aacgcgacga ttacaaaccg ctgtggcgta
    29881 ctccggcttt cgggctgatg accaccctcg ccattgcgtc aagcctcatt ctggtcgcct
    29941 caacctttgt ctgggcaccg attcccggcc ttatctgcgc cgtcatcgtt attgctactg
    30001 gtctgcctgc ttacgccttc tgggcgaagc gtagccgcca gctcaacgct ttgtcgtaat
    30061 gttatctgaa gaaaataaaa tgttggatat tgataaaagc accgtggact ttctggtcac
    30121 cgaaaatatg gttcaggaag tggaaaaagt tctcagccat gacgttccgc tggtgcacgc
    30181 catcgtggaa gagatggtga agcgcgacat tgatcgtatt tatttcgttg cctgcggctc
    30241 gccactcaac gcggcgcaaa cggcgaaaca tctggcggat cgcttttccg atcttcaggt
    30301 ctacgccatt tccggctggg agttctgcga taacaccccg tatcgcctcg acgatcgttg
    30361 cgcggtgatt ggcgtttctg actacggtaa aacggaagag gtaatcaaag cgctggagct
    30421 gggccgggcc tgcggcgcac tcactgcggc gttcaccaaa cgcgcggaca gcccgattac
    30481 ctcggcggcg gaatttagca ttgattatca ggccgactgt atctgggaaa ttcacctgct
    30541 gctctgctac agcgtggtgc tggagatgat cacccgcctc gcgccgaacg cggaaatcgg
    30601 caagatcaaa aacgatctca agcagttgcc gaatgcgctc ggtcatctgg tacgcacctg
    30661 ggaagaaaaa ggccgccagc ttggtgaact ggccagccag tggccgatga tttataccgt
    30721 tgctgcgggt ccgctgcgtc cgctgggtta caaagaaggc attgtaacgc tgatggaatt
    30781 tacctggacg cacggctgcg tgattgagag cggagagttc cgccatggcc cgctggagat
    30841 tgtcgaaccg ggcgttccgt tcctgttcct gctcggcaat gatgaaagtc gccacaccac
    30901 cgaacgcgcc attaactttg ttaaacagcg tactgacaac gtgatcgtca tcgattacgc
    30961 cgaaatttcg caagggctgc acccgtggct ggcaccgttc ctgatgttcg tgccaatgga
    31021 gtggctctgc tactacctgt ctatttacaa agatcacaac ccggatgaac gccgctatta
    31081 cggtggtctg gtggaatatt aatccctctc ccggcccggc aacgggccgg atttacgcaa
    31141 ggagttaccc gatgaaaaca ggtatgttta cctgcggcca ccagcggctg cctattgaac
    31201 acgcatttcg tgatgcaagc gagctgggtt acgacggcat cgaaatttgg ggcggtcgcc
    31261 cgcacgcgtt cgcgccggac ttaaaagcgg gcggcatcaa acaaatcaag gcgctggcgc
    31321 agacgtatca gatgccgatt atcggttaca cgccagaaac caacggctat ccgtataaca
    31381 tgatgctggg cgatgaacat atgcgtcgtg aaagcctgga catgatcaag ctggcgatag
    31441 atatggcaaa agagatgaac gcgggttata cgctgatttc tgcggcccac gcgggctatc
    31501 tcacgccgcc taacgttatc tgggggcggc tggcagagaa cttaagcgaa ctgtgtgagt
    31561 acgcggaaaa catcggcatg gatctgatcc tcgaaccgtt aacgccgtat gaatcgaacg
    31621 tcgtgtgtaa tgctaatgat gtgcttcatg cgctggcgct ggtgccttcg ccgcgcctgt
    31681 tcagcatggt cgacatttgc gcgccgtatg tccaggcgga accggtgatg agttatttcg
    31741 acaaactggg cgataaatta cgtcatctgc atattgtcga cagcgacggg gccagcgaca
    31801 cgcattacat tcctggagaa ggcaaaatgc cgctgcggga actgatgcgc gatattattg
    31861 atcggggcta tgagggttac tgtacggtgg agctggtgac gatgtatatg aacgagccca
    31921 gactctatgc ccgccaggcg ctggaacgct ttcgcgcgct gctgccggag gatgagagat
    31981 gaaaaccctg gcgacaatcg gcgataactg cgtcgatatc tacccgcaac tgaataaagc
    32041 gttttctggc ggtaatgcgg tcaatgtggc ggtgtactgc actcgctacg gcatacagcc
    32101 gggatgcatt acctgggtgg gtgacgatga ctacggcaca aagctgaagc aggatctcgc
    32161 ccgcatgggc gtcgatatca gccatgtcca tacgaaacac ggcgttaccg cacaaactca
    32221 ggtggaactg cacgacaatg atcgcgtttt tggcgactac accgaaggcg tgatggccga
    32281 ctttgccctg agtgaagagg attacgcctg gctggcgcag tatgacattg tgcacgcggc
    32341 aatctgggga catgcggaag acgcattccc acagctgcac gctgcgggca aacttaccgc
    32401 tttcgacttc tccgacaagt gggacagccc gctctggcag acactggtgc cgcatctcga
    32461 ttttgccttt gcctccgcac cgcaagaaga cgaagcgctg cgtctgaaga tgaaagcgat
    32521 tgttgcccgt ggcgcaggaa cagtgattgt cacgctgggt gaaaacggca gcattgcctg
    32581 ggatggcgcg cagttctggc gtcaggctcc tgaaccggtg acggttatcg acaccatggg
    32641 tgccggagat tcgttcattg ccggattcct ttgcggctgg tctgcgggga tgacattacc
    32701 gcaggcgatg gcgcagggaa cggcgtgcgc ggcgaaaacc attcagtacc acggtgcctg
    32761 gtaggtataa cgttggcgtg agcatcttca cgccaacgtg ctgttacttg ccggaaaacg
    32821 accctataat ccgagtaatt cattctttat ttcagggtcg attatgtcag ctacggaccg
    32881 ctactctcat caactcctct acgctaccgt ccgccagcgg ctgctggatg atatcgcgca
    32941 gggggtttac caggccgggc aacagatccc taccgaaaac gagctttgta cacaatataa
    33001 cgtcagccgc attaccattc gcaaagccat cagcgactta gtggcagacg gcgtactgat
    33061 ccgctggcag ggaaaaggca cctttgtaca aagccagaaa gttgaaaacg ccctgcttac
    33121 tgtcagtggt tttaccgatt ttggcgtctc acaaggcaag tcgacgaaag agaaagtgat
    33181 cgaacaggaa cgggtcagcg ccgcgccgtt ttgcgaaaag ctgaacatcc ccggaaacag
    33241 cgaagtgttc catctctgcc gggtgatgta tctcgataaa gagccgctgt ttattgatag
    33301 ttcatggatc ccgctgtcgc gttatcctga ctttgatgag atttacgtcg aaggaagctc
    33361 cacctatcag ttatttcagg agcgttttga cacgcgagtg gtcagcgaca aaaagaccat
    33421 cgatatcttt gccgccaccc gcccgcaggc aaaatggctg aaatgcgaac tgggcgaacc
    33481 gttgtttcgc atcagcaaaa tcgcctttga tcagaatgac aaaccggtgc acgtctccga
    33541 actcttctgc cgcgccaatc gcatcacctt aacgattgat aataaaagac attaaccgta
    33601 ggccggataa gatgcgccag catcgcatcc ggcgatgctg gcgcactgaa ttttacatcc
    33661 cgcacgttcc cctcacccta accctctccc caaaggggcg aggggaccgt tcagtgcgga
    33721 atttagcttg attgccgaac ggtgctatcc agcattaaat actcgcaata ctctcacgca
    33781 aaatccgcag caccgtctct tcaccgctgc gattcgggtt aatgcgaatc gcacaatgtt
    33841 cgagttgtgg attcgcctgg cgaaacgttc ctgaaagtcg atagaagagc ggcgggattt
    33901 catatttcga ctctgcaccg accgggtaag gcaacgcgcc acgcttttgc gcttcttcca
    33961 gcaccctggc ggcaatcagc tgatgaaact cgacaatcaa caccttcgac tgcgcattgg
    34021 caatgacggc gcttttcact tccgccaccg cgccgccgtt aagcaaagcc agcaaccgtt
    34081 cggatactcc cgcctgcacc gcatgcatca ctggcgcgaa caccagacca cgcaagactt
    34141 ccagcgcctg tgagccctgg atctggctac cgccggagta aagcgtggcg cgaatccgac
    34201 taataacatc ggcatcgcca accactgcac caacgccctc cggcccaaac agcttgaagc
    34261 aggagaatgt cgagacattc gcgccgcatt cacagccgat tcgcgctacc ttcatcaccg
    34321 catagttatc atcggttaac gctggaacac ctgccgcgcg caacgttgcc agcacatctg
    34381 ccagcacata gctgtcctgc ggctgctggc gcgtatgctg caccagcgcg gcatcaggtt
    34441 gttgctcgtc gacgacctgc ttcagtgccg acaggtcatt gaaatcagca gtaataagcg
    34501 tcagccccat ctgctcaata ataacccgtg tcgtcgggta aacaggcgcg tcatgcacca
    34561 gaagacgctg ccccggtttg agcaaagccg ccagcccggc gcgaatcgcg ccagtccccg
    34621 cgccctgcac cagcgccgcg gcctgtgcgt gaaatgcatc agccagcacc tgctccaccc
    34681 gttgggtaat acgcggttga ttcagacttg gagttaaccc taaatcaccg ccagcaagaa
    34741 actcgctgcc tggaaaatgg cggcaaatag tgtccaccag cgcaaactgc ttttgctgcg
    34801 cttcagcaag agtcaggctt tgcaaaggaa acgtcttcat aggtgccttc cttaagccgg
    34861 aacgaacagg ccaagccagt aaagaacatt cagcagaata ccggtgatca ttactgccac
    34921 gaccggtgcc gccattttct gtaccggacg gcccagggat tcgttgagga agtaaatggc
    34981 aaccgcgata gagaatccgg tataacccgc catcttaatt gctgcgaaaa tcgaaccgac
    35041 cagcagcgcc acttccatca gcatattcat ggcgttacgg atgttatccg acgcattacg
    35101 caccgacggg tagcgtccca gccatttgcc gatcgaacga agcagcaaga cttccgccga
    35161 aataaccact gcgcctaata ccgctgcaac catcggattc ggcgagagat agcccaccgc
    35221 ataaacaaag gtaaagcccg caactgcata cacacccgtt gctaaggcgg tggtggcaat
    35281 caacggcaca aaccccagtc cgcgcataaa ttctgccaga gccgcctgat taatcagcgt
    35341 ttgcgattgt tccggcgtca cgcctgcgga atacgctttc tccagtgtga agatcgaaac
    35401 ttcgctacca gcaaaaatct tcatactggc aacggcggca atcaatgctc ccacgatggc
    35461 gatataaggt aagtttttga taatccgcga cgtgcgctct tcaaacaccg aaagcccgct
    35521 ggcatcgatg tcattttcat cacgatgacg caggtcgtga gttatcgcga tccccagcag
    35581 catcaccatg ccaataaaga tttcgatgga ttcagggtta agatgtggga aatagcgcac
    35641 gactaccaca cgggtcatca gaacaaccac ggcggcgacc agactttgct tccagccaaa
    35701 ctggtagaaa atcgccacca gcgggaacag tgcaaaagca gaaaccaccg gcgagcttaa
    35761 ttccccgagg ctacctaata catccaccgg cagcgcggtc agcagctggt taactggcaa
    35821 caggcaagta aggatcaaca cgccccagat agcgccaaga ccaaacgcca tcaggctgtt
    35881 tatcgccagt acgccgagga tatcggtagg aagaaagagt aaccatgcgt tgagcagccc
    35941 ggttttcagg gtaaaagaga tccccaccga ggccacaaaa ccaatgctca gaccaaaagc
    36001 gatactcccc gcctcgcgac ggttcatata gccttcaatc agttgcggca ggatcgggtg
    36061 gatgccgtca tgaaaaacag ccgccgagcg atgcgccaga agcgatgtca tacccgtcag
    36121 gcacgccacc acgataatct gaatatacag atccatagtc gtgcctttta tttcagatgg
    36181 ttaatcaaca tcggaatcgc gtgttcgacg tgctcaacgg aaaggccaaa cgccactttc
    36241 ccttcagcga tcattttggc gatatgctcg tctttcgctt taatgcctgg tttggcaatg
    36301 gtgcaacttt tgttatagcc aatcaccgcg atggcaatcg acaatgccgc gcccgcaccg
    36361 gtattacacg cgccaatgta ataatctagt tgcccggatt tcaccttcat tgccgcttcc
    36421 atgtcgttgt gaatgaaaac ttcaaaacag ccaggagccg tcgcttcaat ggttttttta
    36481 atctgctcac gctgtaagcc tgcaacgcca atctttttca tgagtctgtc cttattggaa
    36541 aaattgagag ggattttcac gtaacatcac atccacatcg gcctgactga atcctgactg
    36601 gcgcaattgc ggaataaagg tggtcaataa atagtcatag ccataaccac cattggcttt
    36661 taaatgggag cggcgcgtaa tatccatcga cagcatgacg cggttcagca acccacggtc
    36721 acgtagcgcg tggagcatcg caatgcgctt ttcgtccggg tagtaactgt tcttaccaat
    36781 ggtgtcgaac tgcacgtacg cgccgagatc gatcatcttc aaaatgttgt cgaggttgtc
    36841 tttcagatcg cagtgaccaa cggtgacgcg cgaaagatca actccgtggg cttgcaacag
    36901 agccagttgt tccagcccca tggtgctgaa cgacgtatgc gtggagatcg ggcgtccggt
    36961 ctggttatgc gccagcgcag cggcaataaa taccttctct tccagcggcg taatctttcc
    37021 ttcgctggag ccgatctccg cgatgatccc ggctttcagc tccgtgccat cgataccctg
    37081 ttcaatttca tcgaccatct cctgcgccag ttcctgcacg ctgcgggtca ccacatgttc
    37141 cgggaaaaac gcatcctggt aataaccagt acaggccacc acgttgatcc cggtttcgcg
    37201 cattacatcg agcataaatt gcgcattgcg ccccatgtaa cggttggtca tctcaatcac
    37261 attacgtaca ccccgggtca tcaggtcgtt catctcctgg caaatgaacg catactgatc
    37321 aaggcggcag tccacgttgt ttttaaagcc ggagagatca atatgcagat gctcatgggc
    37381 cagggtgtaa cccgtcggat caaaactcat agggtgtctc ctgcaaaccg gagggaggaa
    37441 agaaaagagc gaccattttg tggcggtggc gcacggaaaa attcacacac ggtagctccc
    37501 acatcagaaa gcgtggtgcg cacaccgagc tgcgtggcga ccagcccttg ctgataaacc
    37561 agcactggca ccacttcacg ggtatggtgg ctgtgaccaa tggtcggatc gttaccgtga
    37621 tccgccatca cgaccaggca atcatctggc tgcatcgcct caacaagccg ggcaaggtta
    37681 cggtcaacga cctgcaaacg ttcggcataa cgtgcgacgt cttctgcatg accagcgagg
    37741 tcggtttcct ggatattgat gcaaataaac gccgtcggat gggtgttaaa ttcgtcgagg
    37801 gtgatatcca taatccgctg gctatccacc aggttttgcc agctcacacc ataaggattg
    37861 ctgacgatat ctgccacctt acccaccagc acggttggca cgcccgcttc atacagtttt
    37921 tgcggcacct gcactttttc atcaacaccg tagcccatat gcacgacctg gaagccgttg
    37981 tcataagcgc cagaacgcgg cgcattaata ccaataaagc gcccttcttt gctttctgcg
    38041 gcatcgagaa tacgctgact gttggttaac aggccgccaa atgtaatgac ccgaccgacc
    38101 tgtacctggt cacgcacgat acgaccaatt ttgattgcgt cgtcaaaaga gatcacagag
    38161 agattggcgg taatgttata gacttggcct aaatccgcct cgagattatc gccaatcgca
    38221 accgcctgat tgaccgacag aaattgcaga tcatcgccac ggcgctccac ctgccagcca
    38281 gcggaaacta atgcctgctc aacacggtca atcacatcgc gaaaaggcat ccgcagcggc
    38341 ggtaacgggc gcgtgcctaa aatttcctga tgccccataa aggtatcgcc accttcatgt
    38401 tgcagctctg ccacgcccca ggttgcggaa tctgacggct gcatatcgcc tggcgcataa
    38461 cccaatgtgt tgattagccc cagcttctcc agcgttggta gctgcaaatg cggcaactgg
    38521 ctcaggatgt gaccacaggt attcgccccg gcatcttgcg ggcgcaccag cgtgacatct
    38581 ttcattgccc ctacgccaaa gctatcaatc actaacacca caaatcgcgc catttatgcc
    38641 tccagagggt tgccgaggct gtcataacga ccaactattt ctggttcgcc gcgctgaata
    38701 ccggacacca gcaccacatc gctacgggtg acaaaaatct gcgtgcgaaa acagagcacc
    38761 actgcactgc tcaccggata ctcgcctgcc agcggcaggg tgtagtcgat actgctgtca
    38821 tccacagttt tgagattggt ttcagtaatc ttttgatttt ctggcgtaaa caccagcgca
    38881 tgttgcgcat gaccacgacg gtagtaaccg ccgccgtagc agtagctgtc gccacggaaa
    38941 tgatgggaga tttcacttaa ccagagcatc gcgatacgtt caggctgatc gccctgctgg
    39001 tttgccggaa tagtaccggt cagcgcatga ccgggttcgg catgagtcgc accgtattcc
    39061 gccagcaatg gcagcgaagt gcagctggtc gctgaaggta cgttcagttg ctcaatcgca
    39121 ataccagatt tcgccagttg atcccgtgct tgtaccagcg tgtgaagatt cggtgttggc
    39181 aaaactttcc cagcagcctc atcccaaagc aggcaaggga aatgggttag tccggctaaa
    39241 tgcagccccg gcagtttctt aatttcagca atcacctcgt gcagcgaatg ctgtacaaaa
    39301 ccgctctcct gccccggata aagaaaatca tcgtcgctat aaactttgag cagcacaggc
    39361 tggacccgtc cggccttaac cgctgccgca gaaacttccc gcgctttgtc gagagtaaac
    39421 acggtgatga tgtcggtgcc ctgttcaacg gcgtcagcaa cctgatgaca agggatttgt
    39481 accagatgcc cctgatgcgc cactggcaaa ccagcgcggc gcatgactcg cgcctctttg
    39541 taatccaccg ccacaatgcc gctgtagcct aatgccagca atttttccgc cagccacgga
    39601 ttacgaccaa attgcttggt catcagatac agttcaatgc cgtaaagccg cgccgtctca
    39661 atgagccgct taccgttttc cagtatctga tccacgtcga tcacccagct gtccggggcg
    39721 attttgccct gctgccacaa acttagtgcg gcggaaatca gcccgggttc tggcgtttca
    39781 atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata ttcatttttt
    39841 tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt cgttagcagc
    39901 catccataat ccataaagat tggccagaaa atagccttct tcacacgggt taacttccag
    39961 agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt ggctggattg
    40021 cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac ggcgactgcg
    40081 catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc gcaccggcag
    40141 atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga cgtccttatc
    40201 aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg cctttcgttt
    40261 aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc agggcgtgtt
    40321 tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc gtcaggacca
    40381 cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc tctaatccca
    40441 gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca tcgcctttaa
    40501 caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca ctatcgccaa
    40561 aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct actcgcttaa
    40621 catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac gtatgcgggc
    40681 caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc gccaggcggg
    40741 aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata tccgcgccgc
    40801 gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg ctcgctaaac
    40861 cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg atatccacat
    40921 gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc ccacggcgtt
    40981 caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc aggccaaccg
    41041 atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg catttttcac
    41101 ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata aacgttcgac
    41161 gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa gataagtggc
    41221 gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt gcgaaaagag
    41281 gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta ttcgacaatg
    41341 cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac cctctcccca
    41401 aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa aaccaatcgc
    41461 ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg cgccgtcttt
    41521 catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt cctgcaattc
    41581 agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat acatcttgcc
    41641 ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa ggatatccag
    41701 caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg gctcgtcgga
    41761 gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca gcaggccgat
    41821 aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca gcagcgacat
    41881 tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct cgccatacag
    41941 cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct ggtcttcacc
    42001 caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca ccggatagcc
    42061 aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa actgcgtcat
    42121 gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact gcgcatgttc
    42181 tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac aagccagata
    42241 caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc gcacggtgat
    42301 cgcgtgttgg tcaacgatac agtaaacgca atggtagtca tcctgcatgt ttacccactg
    42361 acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg cgccactaaa
    42421 aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc cctaatgcgg
    42481 gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg atagcctcgc
    42541 cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt gccgcctgaa
    42601 tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc atccgctcag
    42661 ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg ccgccgataa
    42721 ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg aacggcgtcg
    42781 gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc aacgtatcgg
    42841 caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag cgatcgaaca
    42901 gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg ggcggtttcc
    42961 ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg cgctccatca
    43021 gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg acgggcaact
    43081 ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg tcgaccagcg
    43141 taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg acttaccttt
    43201 gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc gaagattgcc
    43261 gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt gttcaccttc
    43321 acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg gcgacgtact
    43381 tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc agggttgacg
    43441 gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct cagaggtgtc
    43501 gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg cagcgtgcgg
    43561 tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc ggcagcgaaa
    43621 tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg ggcggtaatg
    43681 ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg aacatagtgg
    43741 ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt tgcggtatct
    43801 tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata ctgtttcatc
    43861 cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg tacaaagcca
    43921 gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt cgaactttga
    43981 cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg tgattggaga
    44041 tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga tgcgcttgtt
    44101 gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca gacagcggtg
    44161 cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg ctatcggcgt
    44221 aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac aactctgctt
    44281 ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg cgcagattgt
    44341 aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc gcccgacgga
    44401 acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg gcgcaatttg
    44461 tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc atcttcacaa
    44521 tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga gaaaagtcgg
    44581 tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg cccttgggca
    44641 aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc aaaaaagcgc
    44701 gatttttctt catgccgatt aactaattac accttctccg gctgtggaga aagctccgac
    44761 agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga taaccttgat
    44821 tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt ggcctgaaca
    44881 tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta cacgccagaa
    44941 accaggacat accacggctg accattacgc gtcgtttcat agacaacgta gtttttcaga
    45001 ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct cagctgcaga
    45061 gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt cttccctcca
    45121 gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc cgtagtcgcc
    45181 gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc cacaggggca
    45241 gctggttcag tacgcttcgg cgtctgtgct accggcttcg gctccgtttt cacggttgct
    45301 tgcggttttt tcggttcaat caccgcctgc tgacgaactg ggcgcgtagt ggacggacgt
    45361 tcagcggttt gcgttttcgc cgtgtcacgc gatgcattgc cattgcgaac aggcgcaacc
    45421 gttgcgggtt cagtcggcaa cgtggaattg accgccacat tgttcagctg ttgctgattt
    45481 tgtggctggg tcagcgcatt gttcaggtca ccctgcactt caacacgttg ttgaccatcc
    45541 gttgccaccg gggtttgccc ttgagtcggc gtagaagaga tcggtggcag agaaacatcc
    45601 tgctgagtat tttccgcaga cgtggttccc ggcgctggct gcacgccatt cgcctgatcg
    45661 gtcgcattgc cagcaagatc aatactcttc tcgccagacg cggtttgatc gctggaagtg
    45721 gtcgaggggg cttttagcgc agaaccgata ccgatgatca acagcagtag aaccagaatg
    45781 ccgacgccca tcatcatata ctgacgagaa gcgggtttac tggctgcttt tttgcgctta
    45841 cgcggacgac gctctacgcg ctcttcatcc acggtttcat cttcggattc gtcaatttct
    45901 tcttcgattt ccggttcctc attgcgctct ttttgcgcac gagtcggacg gcgatcgtca
    45961 gtgtcatcaa gttcaatatc atcaaaattg atctgcggtt cgccacgttc agtacgctca
    46021 gaacgttcag aagattgacg agaacgacca gtacgacgat cgctgggatc gggtttcagc
    46081 tcgtcttctg gtttgaattc atccatttaa caccccacta aaaggttaat gcttaccacg
    46141 ttgcaattaa cctgaagcta atagaccgct tgataagcgg cctgaccttt cttgttgtta
    46201 cgctgattga caatcggcaa tggcgttaag aacaagctcg tgcgaaacgc cgctgcgaac
    46261 ttcactctta ccaattgcca acggaagaat taagcgcatc tctcccgcaa ggactttctt
    46321 gtcacgcagc atatgcggta aatacgcctg cgcggacatt tcgcgcggcc cattgaccgg
    46381 taacccagcc cgcgtgagca gggttataat acgctgcgtt tcggcagaac taaactgccc
    46441 gagacgttcc gacgtccgcg ccgccatcac catacccgca gcgaccgctt caccatgtaa
    46501 ccaattgcca taccccattt cagcttcaat ggcatgacca aaggtgtgtc ccagattcag
    46561 taaagcacgt aacccggttt cgcgctcgtc ggcggcgaca acttctgcct tcagttcaca
    46621 acaacggcga atacagtacg ccattgccgg accgtccaga cgcaacaacg catccagatt
    46681 ctcttccagc cagttaaaaa acgcaccgtc aagaataatg ccgtatttga tgacttctgc
    46741 cagccccgac gctaactcac gcgggggaag cgttttcaga cagtcgagat ccaccaccac
    46801 tgaagcaggc tggtagaacg cgccaatcat gtttttaccg aggggatggt tgaccgcagt
    46861 tttgccgcca acggaggaat cgacctgcga cagtaacgtc gtcgggactt gaatgaaacg
    46921 aacaccgcgc tgataactcg ccgccgcgaa gccggtcaga tcgcccacta cgccgccgcc
    46981 aagcgccacc agcgtagtat cgcgaccatg cggcttttgt aacaacgccg taaagacggt
    47041 atcgagtaca gctaggcttt tatactgctc gccgtcaggg aggataacgc tatcgacgtt
    47101 aacacccgcc tgttcaagta cgccgcggac cttatcgaga tacagaggag ccagggtttc
    47161 gctggtgacc aacatgacct gctcgcccga tttcagcggt aagaatgaag ctggttcatt
    47221 aaacaaacca gatgcgatgg taattgggta actacgttcc ccgagagtaa cgacaatcct
    47281 ctccataacg cgacatccac cttaattact gtacccgcag acgagtgtat ataaagccag
    47341 aattagttgc tttccagcat gtgaataatc tggtttgcaa ccactttagc gctttgatca
    47401 tcagtacgaa tggtcacgtc ggcaatctct tcatacagcg gattgcgttc attggccaac
    47461 gcttccagaa cttcacgcgg cggtgtttca acgtgcagca acgggcgttt tttatcacgc
    47521 tgcgtgcgtg caagttgctt ttcgatggtc gtttcaagat aaacgacaac gccacgagcg
    47581 gaaagacggt tacgcgtttc acgggatttc acagagccgc cgccagtagc cagcacaata
    47641 ccctgtttct cggtcaactc attgatgacc ttttcttcgc gatcgcggaa gccttcttcg
    47701 ccttctaaat cgaaaaccca gcccacatca gctccggttc gtttctcaat ctcttgatcg
    47761 gaatcgtaaa attccatatt gagttgttga gctaactggc gcccaatagt gctttttccg
    47821 gcacccatag gcccaaccag aaagatattg cgtttctctg ccattttttc ggtactacta
    47881 agactattcg ttaatgataa acccgcttcg ctcagagagc gccgcaggac atgaactgaa
    47941 acctcataag atattgcgag agtcagactg aaaattatct caatactcca gcgggtttgg
    48001 caactgaata aatcacccat ccctctgcat atctggtcgc tgcaagcgcg ctgccttgct
    48061 accaccgctc tggcgataaa tcaccgggta agattagcgt aaaaaagaca gcaaaatgcc
    48121 gcctgaatga taaatcatca tcatggaacg caggcagcga agagcaccaa ctctcaaatc
    48181 ggtactcctt gtatgctaaa tacctgcgcg cgtcaaatag atgaaacacg ttcagcataa
    48241 aaacattacc gcttttacgg ctgtttactc actggaaacc agtcgtggcg tgataaacac
    48301 cactaactcg cgtcgttcat cttcttttcc gtcatgacga aataattgcc cgaaccaggg
    48361 aatgtcgcca agcaacggta cgctatcctg acccgattta tttttacggg taaaaatgcc
    48421 gcccagcgcc aacgtttctc cgctttttac ctcgacctgc gtttcgatct cctgcttatc
    48481 aatcgccagc acttcgccat cggcctgctg tagcacctgc cccggaacgt tctggctgat
    48541 gtgtaatttc agccggatgc gacctttttg taacaccgtg ggcgtgacct ccatccccag
    48601 gacggcctct ttaaattcca ccgacgtcgc gccactttcc ccgctggaaa cctgatatgg
    48661 aatttctctc tcctgtttaa tgctggcagg ctgaagatgt gaggccagca gacgcggact
    48721 ggcgataata tccagctgct gtttttgttc gagcgcggaa agctcaagat ccagcaagcg
    48781 tccgttgatg cgcccaatgt taaaaccgac atgcgttgtc gccgtcgcta cggagaggtc
    48841 gctaccaagc gtggtgactt gcccaacgcc accagcgtgt tgcgcatcgg ccagcgtcca
    48901 tttcacgcct aactcacgca aacttttttc attaatggtg acaatatgcg ccgacagctc
    48961 aacctgcccg accggcagat ccatttgcgc tacccactgt tcaagcgcgc ttaacgccgt
    49021 tttgttatct cgtagcaaaa ggcgattggt gcgtttatcg acggtcatac tccctttggc
    49081 actcagtagc ttctcccccg ctttcgccag ttctcccgcg tcggcgtatt gcagggttat
    49141 actgcgattt tccagcggca gatttgcctg cgcccgcgcc tgctccgcct cctggcgggc
    49201 gatattgtca ttctgccagg caatggaatg cactgagaga atgttgcctt cctgccgcgt
    49261 tatcagtccg gcgcttttca ctacagtttg tagtgcctgc ttccagggaa catctgttag
    49321 atgtaacgac accgtaccgc tgacgtctgg cgacacgacc aggttcaact tctcctgttc
    49381 agccagcgcc tgcaacacct gagctaccgg aacgtcatcc accatcagcg tcactttttg
    49441 cggctttgcc gcctgtacgc cgggtatcag catcaacagt agtgcggcta tccattgctt
    49501 catttgtatc tccttgccgt tgccacaacc attgtggcgg ttcgcagttt gtcccggtac
    49561 ccagcgttag tacgtctggc gtcagctgta aaattgtcca gccgttttcc agcacatcgt
    49621 tttgctgcac ccgtcgccat ttcttttgcc cgtcttttat tacaccgatg atgcgctcgc
    49681 ctcgccctac catcccctga tagcgccact ggctaagttc gctaatccgg catagatctt
    49741 ccggcgattt aaaagggtca cgcataccgg ttaaaaggca caatgcaata cctgccaaca
    49801 accagcgttt aaccctcatg cggcatctcc agttgtagcg tgaacaaaag atcatcacct
    49861 tccacgctta acgaaaaacg gctcacgctg acgttgcgct ctgccagccg tgtaaatgcc
    49921 gacggcactg cttcccacag cgttttcaac gccaactcgc ctccctgcgc ggatggatgc
    49981 caggaaacca gttgcgcgcc ggataactga aaatccagtg gcgaaaaggg cagcgttttt
    50041 tcctcgctga agggagtggt gtctaccagg cgatacagtg cggcccactg ctggtgatga
    50101 cttgcccgta gtcgaattaa tgcgtcgcgc tcttcatggt gtgtcgatga tagaaaaatg
    50161 agcgtaacta acatcagcaa ccagaatgcc cagcaaaact ggcggaggcg gggtgatgtg
    50221 gcgaaccacc agtcaaagaa catgttcatc gctaaccttc cttgttaact gatactcaaa
    50281 ttgccagcgt ccctgcgcat cctgctgcgt ggctccccgc tgattgagat gaaaagacgc
    50341 atcctggcgg agtgacgttt ctagtgcgtt taacgcggta atgcttgttg tcagcccctt
    50401 gatctccagc gttccctgct gccagcttat cgttgtcagc caggcgtgct cgggtaaaag
    50461 cgccgccagt gcttccagcg cagattgcca gtcgcgggta aattgtcgct ggcgctgccg
    50521 ttgcgaacgc tgttcgcgta actgctgttg ctccagcaaa cgtggcttcg ttatctgtaa
    50581 gctgcgggcg agttgttgtt ccgcctgaag caaaactgcg tctatgcgcg cttcggcgct
    50641 gcctgtcaga cgcagtatta gcgttatccc gacggccagc agcagaggcg caacgaacat
    50701 cagcaaccag aaacgcagaa aagcggtccg gcgttgctgt cgccagggca aaaaattaat
    50761 tggcgggttc atcagtacgc cttcccaagc gccagcccca gcgcgatggc aaagtctcca
    50821 ccaggcggcg gtagcggcgg ctgacgaaca gaaacggcct cccaggggtc aaatccgcct
    50881 tcgccacata tcgcgacgct gtcgggatca acggataacg ctgccgccag ctccttcgcg
    50941 ctagtcatcc ccaccgccag tttgcgcccc cagctatagc gcgtcgccca cagccactgt
    51001 tcgttatcac gccaggccag acattgctga tgagaaggta aaaaaggcag gaatcgctgt
    51061 aatgcactgg catccggggt gatcgcacta acatgaacac gcaacctttc tgccagcgta
    51121 agcagcgttg ccagctcttt gctttgcgcg gcagtcacgt tataagcggg gctgagtgag
    51181 tcttcgctgt aatcgaagcg cagggagtcc ggatccatat ccagctcgcg ggccatcgtc
    51241 cctgacagcc aggccgtttg ctcccgctca ccaagggaca tcgacgggcg cggaaatgac
    51301 cgctgtaatg tgcgactggc gggaaacgcc aacataatgt gatgacgctg cggcagttcg
    51361 cgactccacg gtaacaacgt tttagccagc tgctgcgcat caacaatccg cccatctttg
    51421 ataatgtcgt gcgccagcgg caaccgccac cagcgttgca aaaagcattc ttttgcgccc
    51481 cgcacgatcg caaccgctgc cgcttcttgc tgttgtaaat gcaaaccaat ttgccagatc
    51541 ttaaatgtca ttgtgatgat ctccttatca cccgtcactc tgacgggtat atcaatgcgt
    51601 ctggcttgcc tttatactac cgcgcgtttg tttataaact gcccaaatga aactaaatgg
    51661 gaaatttcca gtgaagttcg taaagtattt tttgatcctt gcagtctgtt gcattctgct
    51721 gggagcaggc tcgatttatg gcctataccg ctacatcgag ccacaactgc cggatgtggt
    51781 gacattaaaa gatgttcgcc tgcaaattcc gatgcagatt tacagcgccg atggcgagct
    51841 gattgctcaa tacggtgaga aacgtcgtat tccggttacg ttggatcaaa tcccaccgga
    51901 gatggtgaaa gcctttatcg cgacggaaga cagccgcttc tacgagcatc acggagttga
    51961 cccggtgggg atcttccgtg cggcaagtgt ggcgctgttc tccggccacg cgtcacaagg
    52021 ggcgagtacc attacccagc agctggcgag aaatttcttc ctcagtccag aacgcacgat
    52081 gatgcgtaag attaaggaag tcttcctcgc gattcgcatt gaacagttgc tgacgaaaga
    52141 cgaaatcctc gagctttatc tgaacaagat ttaccttggt taccgcgcct atggtgtcgg
    52201 tgctgcggca caagtctatt tcggtaaaac ggtcgaccaa ctgacgctga acgaaatggc
    52261 ggtgatagcc gggttgccga aagcgccttc cacctttaat ccgctctact caatggatcg
    52321 tgccgtcgcg cggcgtaacg tcgtactgtc gcggatgctg gatgaagggt atatcaccca
    52381 gcaacagttc gatcagacac gcactgaggc gattaacgct aattatcacg cgccggagat
    52441 tgctttctct gcgccgtacc tgagcgaaat ggtgcgccag gagatgtata accgttatgg
    52501 cgaaagtgcc tatgaagacg gttatcgcat ttacaccacc atcacccgca aagtgcagca
    52561 ggccgcgcag caggcggtac gtaataacgt gctggactac gacatgcgcc acggctatcg
    52621 cggcccggca aatgtgctgt ggaaagtggg cgagtcggcg tgggataaca acaagattac
    52681 cgatacgctg aaggcgctgc cgacctatgg tccgctgctg cctgccgcag tcaccagcgc
    52741 caatcctcag ggagcgacgg cgatgctggc ggacgggtcg accgtcgcat tgagtatgga
    52801 aggcgttcgc tgggcgcgtc cttaccgttc ggatactcag cagggaccga cgccgcgtaa
    52861 agtgaccgat gttctgcaaa cgggtcagca aatctgggtt cgtcaggttg gcgatgcatg
    52921 gtggctggca caagtgccgg aagtgaactc ggcgctggtg tcgatcaatc cgcaaaatgg
    52981 tgccgttatg gcgctggtcg gtggctttga tttcaatcag agcaagttta accgcgccac
    53041 ccaggcactg cgtcaggtgg gttccaacat caaaccgttc ctctacaccg cggcgatgga
    53101 taaaggtctg acgctggcaa gtatgttgaa cgatgtgcca atttctcgct gggatgcagg
    53161 tgccggttct gactggcagc cgaagaactc accaccgcag tatgctggtc caattcgctt
    53221 acgtcagggg ctgggtcagt cgaaaaacgt ggtgatggta cgtgcaatgc gggcgatggg
    53281 cgtcgactac gctgcagaat atctgcaacg cttcggcttc ccggcgcaaa acattgtcca
    53341 caccgaatcg ctggcgctgg gttcagcgtc cttcacccca atgcaggtgg cgcgcggcta
    53401 cgcggtcatg gcgaacggcg gcttcctagt ggacccgtgg tttatcagca aaattgaaaa
    53461 cgatcagggc ggcgtgattt tcgaagcgaa accgaaagta gcctgcccgg aatgcgatat
    53521 tccagtgatt tacggtgata cgcagaaatc gaacgtgctg gaaaataacg atgttgaaga
    53581 tgtcgctatc tcccgcgagc agcagaatgt ttctgtacca atgccgcagc tggagcaggc
    53641 aaatcaggcg ttagtggcga agactggcgc gcaggagtac gcaccgcacg tcatcaacac
    53701 tccgctggca ttcctgatta agagtgcttt gaacaccaat atctttggtg aaccaggctg
    53761 gcagggtacc ggctggcgtg caggtcgtga tttgcagcgt cgcgatatcg gcgggaaaac
    53821 cggtaccact aacagttcga aagatgcgtg gttctcgggt tacggtccgg gcgttgtgac
    53881 ctcggtctgg attggctttg atgatcaccg tcgtaatctc ggtcatacaa cggcttccgg
    53941 agcgattaaa gatcagatct caggttacga aggcggtgcc aagagtgccc agcctgcatg
    54001 ggacgcttat atgaaagccg ttcttgaagg tgtgccggag cagccgctga cgccgcctcc
    54061 gggtattgtg acggtgaata tcgatcgcag caccgggcag ttagctaatg gtggcaacag
    54121 ccgcgaagag tatttcatcg aaggtacgca gccgacacaa caggcggtgc acgaggtggg
    54181 aacgaccatt atcgataatg gtgaggcaca ggaattgttc tgattaaaaa ggcgcttcgg
    54241 cgccttttca gttggctgac aaagtgcatt tgtttatgcc ggatgcgggt gaacgcgtta
    54301 tccggctaac aaaatcgtga aaaatcaata tattgcagga accccataag cctgataagc
    54361 gttgtgcatc aggcaaactt cacgcattta cactcgcccc tgccctttca accattcgcg
    54421 cacgaggaac agcgcgctga cattacgcgc ttcgttgaag tcagggtctt ccagcaaatc
    54481 catcatatgc gccagcggcc agcgcacctg tggtagcggc tctggctcat cgccttccag
    54541 cgattccggg tagagatctt gcgcgaccac gatattcatt ttgctggaaa agtaagacgg
    54601 tgccatgctg agcttcttca aaaaagtcag atcgttcgca ccaaatccaa cctcttcttt
    54661 tagctcgcgg ttagcggctt cgtagacgct ttcacccgga tcaattaatc ctttcgaaaa
    54721 acctaattcg taggattcag ttcccactgc gtattcgcgg atcaggatca ggtgatcgtc
    54781 cacaatcggc acaatcatca ctgcttcccg gttggttgga cgcattcgtt cataaacacg
    54841 ccgcacgcca ttgctgaact ccagatccac gctctcgacg gtaaacagtc gggaacgggc
    54901 tacagtttca acattcagaa tggtgggttt ttgtaatgat ttgttcatcg tgggatctat
    54961 gctgtgaaat cagcggttat tgtgcgatat cggacacgct ttcggcaatg tgaattgcat
    55021 gttatttaca tttatgtaac ttaataaata atcgtcctca aatcaaatta aaagtcaata
    55081 ggttgaaata actccaggaa tttgatgata ttccgccttc agagggtttg ctatgatcag
    55141 cggttactgt gatgtgctta atgatgctca agttaagctc cacgcttgcc gatagccaac
    55201 cgcagaatca tgtattgtgt ccggtgcgac tgaccacgcc tgacagacta agtaagatgg
    55261 ggaaagcatg agcaccattg tgattttttt agctgctttg ctggcctgct cactacttgc
    55321 gggatggctg ataaaagtgc gatccagacg gcgtcagctg ccctggacca acgccttcgc
    55381 ggatgcgcaa acgcgtaaac tcacacctga agaacgtagc gccgttgaaa attatcttga
    55441 gagcctgacg caggtattac aggtgcctgg cccaacggga gctagcgcgg caccgatctc
    55501 tctggcgctg aatgccgaaa gcaacaacgt catgatgctg acacacgcta tcacacgtta
    55561 cggcatctct accgacgatc cgaataaatg gcgttactac ctcgattcgg tagaagtcca
    55621 cctgcccccc ttctgggaac agtacatcaa cgatgagaat accgttgaac tgattcatac
    55681 cgattcgctg ccgttggtta tttcactcaa cggtcatacg ctgcaggagt acatgcagga
    55741 aacgcgcggc tatgccttgc aacctgttcc gtccacgcag gcgtcgattc gcggggaaga
    55801 gagtgagcaa atcgagctac tcaatattcg caaagaaacg catgaagaat atgcgctgag
    55861 tcgtccgcgc gggctgcgtg aagcgttgct gatcgtcgcc tccttcctga tgttcttttt
    55921 ctgcctgatt accccggatg tatttgttcc gtggctggca ggcggcgcat tactgctgct
    55981 gggcgcaggt ctgtgggggc tattcgcgcc cccggcaaaa tcctccctgc gagaaattca
    56041 ttgtctgcgc ggtacacccc gtcgttgggg attgtttggc gaaaacgatc aggaacagat
    56101 caacaatatt tcactcggta ttatcgacct ggtctatccc gcacactggc agccatacat
    56161 tgctcaggat ctcggtcaac aaaccgatat tgatatctat ctcgaccgcc acgtagtgcg
    56221 tcaggggcga tatctttcgc tgcatgatga agtaaaaaac tttccgttac agcactggct
    56281 gcgcagtacg attatcgctg cgggctcgct gctggtgctg tttatgctgt tattctggat
    56341 cccgctggat atgccgctga aattcactct ctcatggatg aaaggcgcgc agaccattga
    56401 agccaccagc gtaaaacaac tggctgacgc tggcgtgcgg gtaggcgata cattgcgtat
    56461 tagcggtacg ggaatgtgta atattcgcac ttccggaacc tggagcgcga aaaccaattc
    56521 acctttttta ccgtttgact gctcgcagat catctggaat gacgcccgct cattaccatt
    56581 accagaatct gaactggtca acaaagcgac ggcattgact gaagcagtta atcgccagct
    56641 gcaccctaaa ccggaagatg aatctcgcgt cagtgcctca ttgcgttcag cgattcaaaa
    56701 atccggcatg gtattgcttg atgattttgg cgacattgta ctgaagacag cggatttatg
    56761 ttctgccaaa gatgactgtg tgcgactgaa aaatgcgctg gtcaatctcg gcaacagtaa
    56821 agactgggac gcgctggtaa aacgcgccaa cgccgggaag ctcgatggcg tgaatgtgtt
    56881 attacgcccg gtgagtgcgg aatcgctgga taacctggtg gcaacctcca ccgcgccgtt
    56941 tatcacgcat gaaacggcgc gagcggcaca atcactaaac agtccggccc ccggcggatt
    57001 cctgattgtc agcgacgaag gcagcgattt tgttgatcag ccctggcctt cggcatcact
    57061 ttacgactac ccgccgcaag aacagtggaa cgctttccag aaactggcac aaatgctgat
    57121 gcatacgccg tttaacgccg aaggtatcgt cacaaaaatc ttcactgacg ccaatggtac
    57181 gcagcatatt ggccttcatc cgatcccgga tcgttccggc ctgtggcgct atctcagcac
    57241 cacattgctg ctactgacga tgctgggtag cgccatttac aatggcgtac aggcctggcg
    57301 tcgttaccag cgttatcgca ctcgcatgat ggagattcag gcctattatg aaagctgcct
    57361 gaacccgcaa ctgatcaccc cttcagaaag ccttatcgaa taacacgttt gcgcggcagg
    57421 ttatgctacc ctgtcgcgca aattgcttca ctctggagat ttccctcatg catatcaaca
    57481 ttgcctggca ggacgtagat accgttctgc tggatatgga cggcacgttg ctcgacctcg
    57541 ccttcgataa ctatttctgg caaaagctgg tgcctgaaac atggggcgcg aaaaacgggg
    57601 ttacgccaca ggaagcgatg gaatatatgc gccagcaata tcacgacgta cagcatacgc
    57661 taaactggta ctgtcttgat tactggagtg agcaactggg tctggatatc tgtgcgatga
    57721 ccaccgagat gggaccgcgc gccgtactgc gtgaagatac cattccgttt cttgaggcac
    57781 tgaaagccag cggtaagcag cgaattttgc tcaccaatgc tcatccgcac aacctggcgg
    57841 taaaacttga gcatacgggt ctggacgcac accttgattt attactttcc acccacacat
    57901 ttggttatcc gaaagaggat cagcggttat ggcatgcggt ggccgaagct acgggtctga
    57961 aagctgaaag aacgctgttt attgatgaca gcgaagcgat tctcgatgct gccgcgcaat
    58021 ttggtattcg ttactgcctc ggcgtgacta atcctgattc cgggattgcc gagaaacagt
    58081 atcaacgcca tccgtcactg aatgactacc gccgcctgat cccctcgcta atgtgaagga
    58141 gacgccatga aagagaaacc tgctgttgag gttcgactgg ataaatggct atgggctgcc
    58201 cgtttttata aaacccgcgc gctggcccgt gaaatgattg aaggcggtaa ggtgcattac
    58261 aacgggcagc gcagcaagcc gagcaaaatc gtcgagctga atgccacgct cactctgcgc
    58321 cagggaaatg acgaacgcac ggtgattgta aaggcgatta ctgaacagcg tcgccccgcc
    58381 agcgaggcag ccttgctgta tgaagagact gcggaaagtg tagagaaacg cgaaaaaatg
    58441 gcgatggcac gtaaacttaa tgccttaacc atgccgcacc cggaccgacg cccggacaaa
    58501 aaagagcgcc gcgacctgtt acgatttaaa cacggcgaca gtgaataact gtcacctgca
    58561 agagagatga ttatgccgca acatgaccaa ttacatcgct atctgtttga aaattttgcc
    58621 gttcgcggcg aactggtaac cgtttcggaa accctgcaac agatccttga gaaccacgat
    58681 tatccgcagc ccgttaaaaa cgtgctggca gaactgctgg ttgcgaccag cctgttaacc
    58741 gctacgctga agtttgatgg tgatatcacc gtacagctgc agggcgacgg tccgatgaat
    58801 ctggcggtta ttaacggtaa caataaccag caaatgcgcg gtgtggcgcg cgtgcagggc
    58861 gaaattccag aaaatgccga cctgaaaacg ctggtcggca atggttacgt ggtgatcacc
    58921 attaccccga gcgaaggcga acgctatcag ggcgtagttg gtctggaagg tgataccctg
    58981 gcggcctgcc tagaagatta ctttatgcgt tctgaacagc tgccgacgcg cctgtttatt
    59041 cgcaccggcg acgtagacgg caaaccggct gcaggcggta tgttgttgca ggtaatgcct
    59101 gcgcaaaata cccaacagga cgactttgac cacctggcga cgctaaccga aaccatcaaa
    59161 accgaagaac tgctgacctt accggcaaac gaagtgttgt ggcgtttgta tcacgaagaa
    59221 gaggtgacgg tttacgatcc gcaggatgtg gagttcaaat gcacctgctc gcgtgaacgt
    59281 tgcgccgatg cgctgaaaac gctgcctgat gaagaagttg atagcatcct ggcggaagat
    59341 ggcgaaattg acatgcattg tgattactgc ggtaaccact atctgttcaa tgcgatggat
    59401 attgctgaaa tccgcaacaa cgcgtctccg gcagatccgt aagttcatta atgagtatgt
    59461 ccggcagaga gggctctgcc ggattttacg acttaatggc ctgatgtgct gcgcttacca
    59521 gcgcctatga aataaatcgc aaattatttt ggtcagatac agcgtcgtat ccgataaaag
    59581 cgttgtcact tacttaagtt tatttccagc gtttcaccat ctaccgtcag atcgcgccac
    59641 gctttcggcc tcgtccccag ttttccatcc ttcgcagctg tgagtaattt ttctttcgtt
    59701 atttcgttcg gaagtaaact catatcaagc gcgtcccatt cttttctgtc tgagtcaaag
    59761 ccatagacaa tgactctgtc atcattaaaa gcaaacagga tccgctccgc ctgaccatca
    59821 ctattcagat cttgctcgac cagtacgcag gcgtcttttt caatacaggt catcacgtta
    59881 tagcgatcct ggattaaggc cgaccagaat gtcgcatccg gtttaacaga accaggggca
    59941 attaacacat tttcggcgaa tactttttcc gatacctgtt cctgaagatg ttgctctcca
    60001 tctaacgcca tcagcagatc ccgcgcgcgt ttcgggtctt tcataaaccc ggcatcgctt
    60061 ttcagcgact caagcgccgc acgtccatag cgaccgctct gttcgagcat gtagatagtt
    60121 acctggtctg gcgtgttttt gccgctctga taacgcgcca tatgactgtt cacgctaata
    60181 cgcatactgt ccagcaccgg cgaattaaga agcaccagta tcaccaacac caataacgaa
    60241 accgcaaggt tcactttgcc ctgaagaaca aggggatttt gcccattacg ccagacgata
    60301 ctgacaaaat accccagcga ccacaccagt aacaccagct ccgccagcgc gccctgcaag
    60361 cggtcgacag tccagccgta ctgagcgacc cgcagccata acccccaggt ggcgacgaac
    60421 acatacagcg gagcaaccag caaagcggtt ttaatcaggc aacgcaacgg ccccgtccag
    60481 ggaagtgacg ctttttgcgg atcgcggaca atagccatta agatcaattg caaaaaggcc
    60541 agcgttaaca gcaacccggc ggcggagatg tggcgagaaa tcgcgctcag gcccgtaaac
    60601 ggcagggtga tgataaacat cagggttagc aatgatacta acggcagcaa ccccgtggcg
    60661 attagcgtga acaacttttg aatagagtcg attaaacgtg actgtgtacg cgcgaggatc
    60721 accgccagtg cggtaaccag accaaatgta agatacataa accagtcggt tgcaaaaaag
    60781 agcgtattga aaaacgtgat gccaacaagt ttaaacaact caccccacag taaaagcacc
    60841 agccacgtca ggccattgga gagaaaaatc accagtaata taagtacgtt atgccatact
    60901 gactggtaaa agtagctata gcggctactg ccattgcgga tacgaaggct ttgttgtatc
    60961 cacggtagca acaacattgc catcaacagc agatagcagc caaaatccca aatcgccttt
    61021 tcagctctcc aggggttcat gccatcagtt tgccacttca gccaaccgct catccctgtc
    61081 gtggcaataa acaccagcgc cagccatccc caaaggcgct tctgtttaaa agagatcacg
    61141 gagaagagta aaacggatga aaacgcgacg gtcgcaggca cgccataaac aatccaactg
    61201 tgattttttc cggcaagcca ggcgataagc aggtagcaaa ccagcccctg aagtaatccg
    61261 gtagcaatca tcccccagcg tgttgccggt gaaagttcaa cgttatccat gttggttatc
    61321 cagaatcaaa aggtgggtta attatcgcat ccgggcagta gtattttgct tttttcagaa
    61381 aataatcaaa aaaagttagc gtggtgaatc gatactttac cggttgaatt tgcatcaatt
    61441 tcattcagga atgcgattcc actcacaata ttcccgccat ataaaccaag atttaacctt
    61501 ttgagaacat tttccacacc taaaatgcta tttctgcgat aatagcaacc gtttcgtgac
    61561 aggaatcacg gagttttttg tcaaatatga atttctccag atacgtaaat ctatgagcct
    61621 tgtcgcggtt aacaccccca aaaagacttt actattcagg caatacatat tggctaagga
    61681 gcagtgaaat gcgcgttaac aatggtttgc ccccgcaaga actcgaggct tatggtatca
    61741 gtgacgtaca tgatatcgtt tacaacccaa gctacgacct gctgtatcag gaagagctcg
    61801 atccgagcct gacaggttat gagcgcgggg tgttaactaa tctgggtgcc gttgccgtcg
    61861 ataccgggat cttcaccggt cgttcaccaa aagataagta tatcgtccgt gacgatacca
    61921 ctcgcgatac tttctggtgg gcagacaaag gcaaaggtaa gaacgacaac aaacctctct
    61981 ctccggaaac ctggcagcat ctgaaaggcc tggtgaccag gcagctttcc ggcaaacgtc
    62041 tgttcgttgt cgacgctttc tgtggtgcga acccggatac tcgtctttcc gtccgtttca
    62101 tcaccgaagt ggcctggcag gcgcattttg tcaaaaacat gtttattcgc ccgagcgatg
    62161 aagagctggc aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag tgcactaacc
    62221 cgcagtggaa agaacagggt ctgaactccg aaaacttcgt ggcgtttaac ctgaccgagc
    62281 gcatgcagct gattggcggc acctggtacg gcggcgaaat gaagaaaggg atgttctcga
    62341 tgatgaacta cctgctgccg ctgaaaggta tcgcttctat gcactgctcc gccaacgttg
    62401 gcgagaaagg cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa accacccttt
    62461 ccaccgaccc gaaacgccgc ctgattggcg atgacgaaca cggctgggac gatgacggcg
    62521 tgtttaattt cgaaggcggc tgctacgcaa aaactatcaa gctgtcgaaa gaagcggaac
    62581 ctgaaatcta caacgctatc cgtcgtgatg cgctgctgga aaacgtcacc gtgcgtgaag
    62641 atggcactat cgactttgat gatggttcaa aaaccgagaa cacccgcgtt tcttatccaa
    62701 tctatcacat cgataacatc gttaagccgg tttccaaagc gggccacgcg actaaggtta
    62761 tcttcctgac tgctgatgct ttcggcgtgt tgccgccggt ttctcgcctg actgccgatc
    62821 aaacccagta tcacttcctc tctggcttca ccgccaaact ggccggtact gagcgtggca
    62881 tcaccgaacc gacgccaacc ttctccgctt gcttcggcgc ggcgttcctg tcgctgcacc
    62941 cgactcagta cgcagaagtg ctggtgaaac gtatgcaggc ggcgggcgcg caggcttatc
    63001 tggttaacac tggctggaac ggcactggca aacgtatctc gattaaagat acccgcgcca
    63061 ttatcgacgc catcctcaac ggttcgctgg ataatgcaga aaccttcact ctgccgatgt
    63121 ttaacctggc gatcccaacc gaactgccgg gcgtagacac gaagattctc gatccgcgta
    63181 acacctacgc ttctccggaa cagtggcagg aaaaagccga aaccctggcg aaactgttta
    63241 tcgacaactt cgataaatac accgacaccc ctgcgggtgc cgcgctggta gcggctggtc
    63301 cgaaactgta atgatttgaa gctggagaat atctatccag tatcttatag aaagcaaaac
    63361 gggaggcacc ttcgcctccc gtttatttac ccttcttttg tcgtgccctg cgcccgcgtt
    63421 accggcactg gcagccaggc gcgaatggaa agcccgcccc gctcgctggt gccaagctcc
    63481 agcatcccgt tatggttatc cacgatacgc tgcacaattg ccagccctaa tcccgtgccg
    63541 ctaatggtgc gcgcactgtc gccgcggaca aacggctgga acaggtgctt acgttgttcc
    63601 ggcgcaattc ccggaccgtc atcttccacc tggaaccagg cgcgattcgg ctccgttccg
    63661 ctgctgactt tgatccagcc attgccataa cgggcggcgt tgaccaccat attcgccacc
    63721 gcgcgtttga tcgacagcgg gtgcattttc acttcaatgc tgccggggta aagcgcggtt
    63781 tcaatttccc gctcatagcc actttcggca gcaatcacct caccgagcac tgcattaaga
    63841 tccgccattt ccatcggcat ctcctgcccg gtgcgcaggt agtcgataaa ctgctcaatg
    63901 atggcgttgc actcttcgat atctttattg atcgattctg ccagatagcc atcctgctcg
    63961 ctcatcatct cagtcgccag gcgaatacgc gtcagcggcg tgctcaagtc gtgacttacc
    64021 cccgccatca gcagcgtgcg gtcatccgcc agttgcttaa caccagccgc catatggtta
    64081 aaggcacggg taacggaacg cacctccgaa gcgccatatt cacgcagcgg cggcggaata
    64141 atccctttac caacctgcaa ggctgcgtgt tcgagatcga ccaacggtcg gttctggata
    64201 cgaataaaca gccacgcccc gcctatcgcc aatagcataa tcgccagcgt atagcggaac
    64261 agcggagaga aatcgccctg atgaatttcg gtcagcggca cgcgtaccca gatattgggc
    64321 gacagccagg ttttcagcca gacgacaggc gaacttttgt tgacctcaac gcgcacttcc
    64381 gtcgggccgc ccagttgctg tgccatctga tggcttaaga attcatagtg ttgcgcccaa
    64441 cgcagacctg cctcttcggc agcctcgttg gagtagagag agatccccag ctcacggtag
    64501 atctcccgac ggaaagcggg aggcacaacc aactgcgtgc cgtcctccag ttgcagtttg
    64561 tcggtcatca acatacgcac ttcgtacgcg aggactttat taaactgctg gaggctcggc
    64621 aaaatcgcga agttcagcac caccagataa gtcgtcacca ggctggcgaa cagcaaggtg
    64681 acgatgagca ataacgtacg ggcaaatgaa cttcgtggcg agaagcgcaa tcgcctcatg
    64741 ctttagagcc gtccggtaca aagacgtagc ccaggcccca aacggtctga atgtaacgcg
    64801 gatgcgctgg atcttcttct accatgcggc gcagacgcga aatctgcacg tcgatggagc
    64861 gttccattgc ggaatattca cgaccacggg caaggttcat cagcttatcg cgggagagcg
    64921 gctcacgcgg atggctgacc agtgccttca gtaccgcaaa ctcaccgctg gtgagcggca
    64981 tcggctcatc ttcgcggaac atttcgcgcg taccgaggtt aagtttgaac ttaccgaaag
    65041 caattaccgc ctcttcctgt gacggtgcgc ctggcagttc gttcgcctga cgacgcagca
    65101 ccgcacgaat acgggccagc agttcacgcg ggttaaacgg ttttggaatg tagtcgtcag
    65161 cgccaatctc caggcctacg atacggtcca cttcttcccc tttcgccgtc accataatga
    65221 tcggcatcgg gttgctctga ctacgaagac gtcggcaaat cgacaagcca tcttcaccag
    65281 gtaacattaa atccagtacc ataagatgga aagattcacg agtcagcagg cgatccatct
    65341 gttctgcatt agcgacgctt cgaacctgga agccttgttc ggtgagataa cgttccagca
    65401 gcgcacgcag gcgcatgtcg tcatcgacca ccagaatctt gtagttctct tgcattgttt
    65461 gtactcccaa aggttcgcaa caatttgtaa gcgtgtattc ttaaaaaagc tcacgttcgt
    65521 caccagctaa atctggtatg aatttcagcc taaattgtta caaagcatat taaacagcag
    65581 cttaagtata caatttattc ggcgaaacat tattgattct gttgatatga tcacgttata
    65641 cccaatgtgc gcattatcaa acagacaaag ggaatcaacg agatgaaaac gcccctggtt
    65701 acccgggaag ggtatgaaaa actcaaacaa gagcttaatt atctctggcg tgaagaacgc
    65761 ccggaggtca caaaaaaggt gacctgggcc gcaagtctgg gcgaccgcag cgaaaatgct
    65821 gactatcagt ataataaaaa gcgtctgcgt gaaatcgacc gtcgcgtgcg ctatctcact
    65881 aaatgcctgg aaaatctcaa aatcgtcgat tactcccctc agcaggaagg caaagtcttt
    65941 tttggcgcgt gggtggagat tgaaaacgac gatggcgtga ctcaccgttt ccgtattgtc
    66001 ggctacgatg aaatttttgg ccgtaaagat tacatctcta tcgattcccc gatggcccgc
    66061 gcattgctga aaaaagaagt cggcgatctg gcggtggtga atacccctgc cggggaagcg
    66121 aactggtatg ttaatgctat cgagtacgtg aaaccgtaag gaagagtctt aacctcctgc
    66181 cgatggctgg catttttgcc agccagtccg tataactatc ccctgatttt tgatccgaaa
    66241 agatgaactc aaaccatgat gaatgattcg ttctgccgca ttattgcggg tgaaattcag
    66301 gcgcgcccgg aacaggttga cgctgccgtt cgcctgcttg acgaagggaa taccgtgccg
    66361 tttatcgcac gttatcgtaa ggaaatcacc ggcggtctgg atgacacgca gctgcgtaat
    66421 ctggaaacgc gtctgagcta tctgcgcgag ctggaagaga gacgtcaggc gatcctcaaa
    66481 tccatttccg agcaaggcaa actcaccgat gatctggcga aggccatcaa cgccacccta
    66541 agcaaaaccg aactcgaaga cctctacctg ccctacaaac ctaaacgccg cacccgcggg
    66601 caaatcgcca ttgaagcagg gcttgagccg ttggctgacc tgctgtggag cgatccgtca
    66661 cacacgccag aagtcgccgc tgcacaatat gttgatgccg ataaaggcgt ggcagatacc
    66721 aaagccgcgc tggatggcgc gcgctatatc ctgatggaac ggtttgccga agatgccgcg
    66781 ctgctggcga aagtgcgtga ttatctgtgg aagaacgcgc atttggtttc tacggtggtg
    66841 agcggtaaag aagaggaagg ggcgaaattc cgcgactatt tcgatcatca cgaaccgttg
    66901 tccacggtgc cttctcaccg cgcgctggcg atgttccgtg ggcgtaacga aggcgtactc
    66961 cagctttcgc tgaatgccga tccgcagttc gatgagccgc ccaaagagag ctattgcgag
    67021 caaatcatca tggatcacct tggcctgcgc ctgaacaatg ccccggcgga tagctggcgc
    67081 aaaagcgtag tgagctggac ctggcgcatc aaggtgctga tgcatctgga aaccgaactg
    67141 atgggtaccg tgcgcgaacg cgcggaagat gaagcaatca acgtctttgc ccgtaacctg
    67201 cacgatctgc tgatggcggc ccctgccgga ctgcgtgcaa cgatgggcct cgatccgggt
    67261 ctgcgtaccg gggtaaaagt ggcggtggtc gatgccactg gcaaactggt ggcgaccgat
    67321 accatttacc cgcacaccgg acaagccgca aaagcagcga tgaccgttgc tgccttgtgt
    67381 gaaaaacata acgttgaact ggtggcgatc ggcaacggta cagcttcccg cgaaactgaa
    67441 cgtttctatc tcgacgtgca gaagcagttc ccgaaagtga ccgcacagaa agtgatcgtc
    67501 agcgaagctg gcgcgtcggt ttactcagct tccgagctgg cagcgcagga gttcccggat
    67561 ctcgacgttt cgctgcgtgg tgcggtatct atcgcccgcc gtttgcagga tccgctggcg
    67621 gagctggtga aaatcgatcc gaaatctatc ggcgtaggtc agtatcagca tgacgtcagc
    67681 cagacgcaac tggcccgcaa actggatgca gtagtagaag actgcgtaaa cgccgttggc
    67741 gtcgatctca acaccgcttc tgttccgcta ttaacccgcg tggcgggcct gacgcgcatg
    67801 atggcgcaaa acatcgtggc ctggcgcgat gagaacggcc agttccagaa ccgtcagcaa
    67861 ctgctgaaag tcagccgtct ggggccgaaa gccttcgagc agtgcgcggg cttcctgcgc
    67921 attaaccacg gtgataaccc gctggacgcc tctaccgttc atccggaagc ctatccggtg
    67981 gtggaacgca ttctggcagc aacacagcag gcactgaaag atctgatggg taacagcagc
    68041 gaactgcgta acctgaaagc gtctgacttt actgatgaaa aattcggtgt gccgacagta
    68101 actgacatca tcaaagagct ggaaaaaccg ggtcgcgatc cgcgtccgga atttaaaacc
    68161 gctcagtttg ccgatggcgt cgagacaatg aacgacctgc aaccgggtat gatcctcgaa
    68221 ggcgcagtga ccaacgtcac caactttggc gcgtttgtcg atattggcgt gcatcaggac
    68281 ggcctggttc acatctcttc attatcgaac aagtttgtgg aagatccgca taccgtggtg
    68341 aaagcgggcg acattgtgaa ggtgaaagtg ctggaagtgg atcttcagcg taaacgtatc
    68401 gccctgacta tgcgtctgga cgagcagcct ggcgaaacca actctcgtcg cggcggcggt
    68461 aatgaacgcc cgcaaaacaa ccgcccggca gccaaaccac gcggtcgtga agcgcagcct
    68521 gccggtaata gcgcgatgat ggatgcgctg gcggcagcga tgggtaaaaa acgttaaacg
    68581 cccggactgg cccctaaacc tgcgcgataa ggcgttcacg ccgcatctgg caacgaacgc
    68641 tttgcctgac ataaaagtgc cggagaatat ctccggcact tttattccac agccaaactc
    68701 acaatatact ccgttaatat ttatcacttc attaacaact gaaaccttaa ttaaacatta
    68761 gccagtcctg gtaactcact attcgaatta tattttcgct gcgatataac cttgagccac
    68821 atcaacattg agtcagatta ttattcaaac caacattcgc acacatttta agtattgctg
    68881 atagaaacca ttctcattat cattgtgttg ttgattattt aatctctcct ttgttggcaa
    68941 atcatctggt ctcatgtcgc ggtcaaacgc cccatgaggt agttatccag ttaatgagaa
    69001 acaagtaggc acctatgcaa tacactccag atactgcgtg gaaaatcact ggcttttccc
    69061 gtgaaatcag cccggcatat cgccaaaaac tgctttctct aggcatgtta cctggctcct
    69121 cttttgatgt ggtgcgcgtc gctccactcg gcgaccccat tcatatcgaa acccgtcgtg
    69181 tgagcctggt attacgcaaa aaagatctgg ccttattaga agtggaagcg gtttcctgtt
    69241 aatacggtga taacaacaat gaaaaaatta accattggct taattggtaa tccaaattct
    69301 ggcaagacaa ccttatttaa ccagctcact ggcgcacgtc agcgtgtagg taactgggct
    69361 ggcgttaccg tcgaacgtaa agaagggcaa ttctccacca ccgatcatca ggtcacgctg
    69421 gtggacctgc ccggcaccta ttctctgacc accatctcat cgcagacctc gctcgatgag
    69481 caaatcgcct gtcactacat tttgagtggc gacgccgacc tgctgattaa cgtggtggat
    69541 gcgtctaacc ttgagcgtaa cctgtacctg acgctgcaac tgctggaact cggcattccc
    69601 tgcatcgtgg cgctgaacat gctcgacatt gccgagaagc aaaatattcg tattgaaatt
    69661 gatgctctat cggcacgtct tggctgcccg gtgatcccgc tggtttcaac ccgtggtcgc
    69721 ggtattgaag cgctcaagct ggcgattgat cgctataaag ctaacgagaa tgtggaactg
    69781 gtgcattacg cacagccgct actcaacgaa gcagattcac tggcaaaagt gatgccttcc
    69841 gacatcccgc tgaaacaacg tcgctggctg ggcctgcaaa tgctggaagg cgatatctac
    69901 agccgcgcct acgccggtga agcgtcgcag catctggatg ccaccctcgc ccgtctgcgt
    69961 aatgaaatgg atgatccggc gctgcacatt gccgatgcgc gttaccagtg cattgctgcc
    70021 atctgtgatg tggtaagcaa caccctgacg gcagaaccca gccgtttcac cactgcggta
    70081 gataaaatcg tgctcaaccg tttcctcggt ctgccgattt tcctctttgt gatgtacctg
    70141 atgttcctgc tggctatcaa catcggcggg gcgttacagc cgctgtttaa cgtcggctcc
    70201 gtggcgctat ttgtgcatgg tattcaatgg attggctaca cgctccactt cccggactgg
    70261 ctgactatct tcctcgccca gggcctgggt ggcggtatta acaccgtgct gccactggtg
    70321 ccgcagatcg gcatgatgta cctgttcctc tccttccttg aggactccgg ctatatggcg
    70381 cgtgcagcgt ttgtgatgga ccgcctgatg caggcgctgg gcttgccggg gaaatccttt
    70441 gtgccgctga tcgtcggttt cggttgtaac gtaccgtcgg taatgggcgc acgtacgctt
    70501 gatgcaccgc gtgaacgtct gatgaccatc atgatggcac cgtttatgtc ctgcggcgcg
    70561 cgtctggcta tcttcgcagt attcgcggct gccttcttcg ggcagaacgg tgccctggcg
    70621 gttttctcgc tgtatatgct cggtatcgtg atggcggtgc tgactggcct gatgctcaag
    70681 tacaccatca tgcgcggtga agcgacgccg tttgtcatgg agctgccggt ctaccatgta
    70741 ccacacgtta aaagcctgat tatccagacc tggcagcgtc tgaagggctt cgttctgcgt
    70801 gctggtaaag tgattatcat cgtcagcatt ttcctgagcg ctttcaacag cttctcgttg
    70861 agcgggaaaa tcgtcgataa catcaacgac tcggcgctgg cttccgtcag ccgggtgata
    70921 acccctgtct tcaagccgat tggcgtacat gaagataact ggcaggcaac ggttggcctg
    70981 tttacgggcg caatggcaaa agaagtggtg gtgggtacgc tcaacaccct ctacaccgca
    71041 gaaaatattc aggacgaaga gttcaatccg gcggaattca acctcggtga agagctgttc
    71101 agtgcggtag atgaaacctg gcagagcctg aaagacacct tcagccttag cgtactgatg
    71161 aaccccattg aagctagcaa aggcaacggt gaaatgggta ccggggcgat gggcgtgatg
    71221 gatcagaaat tcggtagcgc agctgccgca tacagctacc tgattttcgt cctgctgtat
    71281 gtaccatgta tctcggtgat gggagccatc gcccgtgaat caagccgtgg ctggatgggc
    71341 ttctccatcc tgtgggggct gaatatcgct tactcactgg caacattgtt ctatcaggtc
    71401 gccagctaca gtcagcaccc aacttacagc ctggtgtgca ttctggcggt aatcctgttt
    71461 aacatcgtag ctatcggtct gctgcgccgc gcgcgtagcc gggtggatat cgaactgctg
    71521 gcaacccgca agtcggtaag cagttgctgc gcagccagca ccaccggtga ttgccattaa
    71581 tggcttcact tattcaggta cgtgatttgc tggcgttacg gggccgtatg gaagcgaccc
    71641 agataagcca gacattgaac actccgcagc caatgattaa cgccatgctg caacagctgg
    71701 aaagcatggg caaagccgtg cggattcagg aagaacctga cggctgcctc tctggcagtt
    71761 gtaaaagctg cccggaaggc aaagcctgtc tgcgcgagtg gtgggcgctg cgttaaccat
    71821 actccatcgc ctgttttgaa aagcccggta tgcgtttgca tccgggcttt tttgcgtgcg
    71881 gctttccata aaaatgcaat tcttgcagcg caacgtaagt tcctctgaaa gcgtctcgca
    71941 gggatgaaaa ctcgctaatg cacagatgtg gagtggcgcg tagagtcgcg gcattcaaac
    72001 aacaggtgaa ggaacgccat gagtaaaaag cagagttcca ccccacatga tgcgctgttc
    72061 aaactctttt tacgccaacc ggagacggca cgcgattttc ttgcgtttca tttaccggca
    72121 cccattcacg cgctctgtga tatgaaaacc ctcaagctgg agtcgaggag ctttattgat
    72181 gacgatctgc gtgaaagcta ttccgatgtg ctgtggtcgg tgaaaacgga acaaggacca
    72241 ggatacatct attgtctgat tgaacatcaa agcacctcaa acaaactgat cgcatttcgc
    72301 atgatgcgtt acgctattgc cgcaatgcaa aatcaccttg atgctggata caaaacgttg
    72361 ccgatggtgg tgccattgtt gttttaccac ggtattgaaa gcccctatcc ctattcgctg
    72421 tgttggctgg attgtttcgc cgatcccaaa ctggcaaggc agctttatgc ctccgcattt
    72481 ccgctgattg atgtcaccgt catgcctgat gatgaaatca tgcagcaccg acgtatggcg
    72541 ctgctggagt taattcaaaa acatattcgt caacgcgatc tgatggggct ggtagagcaa
    72601 atggcctgct tattaagtag tggatacgct aatgacagac aaatcaaagg gctgtttaat
    72661 tacatactgc aaactggcga cgctgtacgt tttaacgatt ttatcgacgg cgttgccgaa
    72721 cgttcaccga aacacaagga gagtttaatg actattgcgg aaagattgcg gcaggagggg
    72781 gaacaatcca aagccctgca tatagccaaa ataatgcttg aatccggagt ccctcttgca
    72841 gacatcatgc gatttaccgg gctgtcagaa gaagagttgg ctgcggcg
//
LOCUS       DAOFMX010000006        70710 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.006, whole genome shotgun sequence.
ACCESSION   DAOFMX010000006 DAOFMX010000000
VERSION     DAOFMX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.006"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..794
                     /locus_tag="QBA20_000518"
     CDS             <1..794
                     /locus_tag="QBA20_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ6291176.1"
                     /translation="LPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDD
                     DNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFA
                     LIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQF
                     VVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYL
                     SGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            805..1896
                     /locus_tag="QBA20_000519"
     CDS             805..1896
                     /locus_tag="QBA20_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6291177.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="QBA20_000520"
     CDS             complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="QBA20_000520"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDQ6291178.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2864..4900
                     /gene="lpoA"
                     /locus_tag="QBA20_000521"
     CDS             2864..4900
                     /gene="lpoA"
                     /locus_tag="QBA20_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDQ6291179.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4858..5253
                     /locus_tag="QBA20_000522"
     CDS             4858..5253
                     /locus_tag="QBA20_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDQ6291180.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5273..5863
                     /gene="diaA"
                     /locus_tag="QBA20_000523"
     CDS             5273..5863
                     /gene="diaA"
                     /locus_tag="QBA20_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDQ6291181.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5873..6448
                     /gene="yraP"
                     /locus_tag="QBA20_000524"
     CDS             5873..6448
                     /gene="yraP"
                     /locus_tag="QBA20_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDQ6291182.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6562..7602)
                     /locus_tag="QBA20_000525"
     CDS             complement(6562..7602)
                     /locus_tag="QBA20_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDQ6291183.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7675..8310)
                     /locus_tag="QBA20_000526"
     CDS             complement(7675..8310)
                     /locus_tag="QBA20_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDQ6291184.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8438..8956
                     /gene="yhbO"
                     /locus_tag="QBA20_000527"
     CDS             8438..8956
                     /gene="yhbO"
                     /locus_tag="QBA20_000527"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDQ6291185.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8936..9379)
                     /locus_tag="QBA20_000528"
     CDS             complement(8936..9379)
                     /locus_tag="QBA20_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDQ6291186.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9430..9732
                     /gene="yhbQ"
                     /locus_tag="QBA20_000529"
     CDS             9430..9732
                     /gene="yhbQ"
                     /locus_tag="QBA20_000529"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDQ6291187.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9719..10222)
                     /locus_tag="QBA20_000530"
     CDS             complement(9719..10222)
                     /locus_tag="QBA20_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDQ6291188.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10216..10740)
                     /locus_tag="QBA20_000531"
     CDS             complement(10216..10740)
                     /locus_tag="QBA20_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDQ6291189.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10949..11944
                     /locus_tag="QBA20_000532"
     CDS             10949..11944
                     /locus_tag="QBA20_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDQ6291190.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11953..12831
                     /locus_tag="QBA20_000533"
     CDS             11953..12831
                     /locus_tag="QBA20_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDQ6291191.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12912..13919
                     /locus_tag="QBA20_000534"
     CDS             12912..13919
                     /locus_tag="QBA20_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDQ6291192.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQTLHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="QBA20_000535"
     CDS             complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="QBA20_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDQ6291193.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="QBA20_000536"
     CDS             complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="QBA20_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDQ6291194.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="QBA20_000537"
     CDS             complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="QBA20_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDQ6291195.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="QBA20_000538"
     CDS             complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="QBA20_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDQ6291196.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="QBA20_000539"
     CDS             complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="QBA20_000539"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDQ6291197.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="QBA20_000540"
     CDS             complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="QBA20_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDQ6291198.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21297..22241)
                     /gene="truB"
                     /locus_tag="QBA20_000541"
     CDS             complement(21297..22241)
                     /gene="truB"
                     /locus_tag="QBA20_000541"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDQ6291199.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="QBA20_000542"
     CDS             complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="QBA20_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDQ6291200.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22806..25454)
                     /gene="infB"
                     /locus_tag="QBA20_000543"
     CDS             complement(22806..25454)
                     /gene="infB"
                     /locus_tag="QBA20_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDQ6291201.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="QBA20_000544"
     CDS             complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="QBA20_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDQ6291202.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="QBA20_000545"
     CDS             complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="QBA20_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDQ6291203.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27653..27729)
                     /locus_tag="QBA20_000546"
     tRNA            complement(27653..27729)
                     /locus_tag="QBA20_000546"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27693..27695),aa:Met,seq:cat)
     gene            28077..29420
                     /gene="argG"
                     /locus_tag="QBA20_000547"
     CDS             28077..29420
                     /gene="argG"
                     /locus_tag="QBA20_000547"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDQ6291204.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29428..31035)
                     /locus_tag="QBA20_000548"
                     /pseudo
     CDS             complement(29428..31035)
                     /locus_tag="QBA20_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31513..31599)
                     /locus_tag="QBA20_000549"
     tRNA            complement(31513..31599)
                     /locus_tag="QBA20_000549"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31563..31565),aa:Leu,seq:gag)
     gene            complement(31614..31946)
                     /gene="secG"
                     /locus_tag="QBA20_000550"
     CDS             complement(31614..31946)
                     /gene="secG"
                     /locus_tag="QBA20_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDQ6291205.1"
                     /translation="MYEALLVVFLIVTIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIMSLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="QBA20_000551"
     CDS             complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="QBA20_000551"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDQ6291206.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33504..34352)
                     /gene="folP"
                     /locus_tag="QBA20_000552"
     CDS             complement(33504..34352)
                     /gene="folP"
                     /locus_tag="QBA20_000552"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDQ6291207.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="QBA20_000553"
     CDS             complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="QBA20_000553"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDQ6291208.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGSEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="QBA20_000554"
     CDS             complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="QBA20_000554"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDQ6291209.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37231..37524
                     /gene="yhbY"
                     /locus_tag="QBA20_000555"
     CDS             37231..37524
                     /gene="yhbY"
                     /locus_tag="QBA20_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDQ6291210.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37680..38156)
                     /gene="greA"
                     /locus_tag="QBA20_000556"
     CDS             complement(37680..38156)
                     /gene="greA"
                     /locus_tag="QBA20_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDQ6291211.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38404..39837
                     /gene="dacB"
                     /locus_tag="QBA20_000557"
     CDS             38404..39837
                     /gene="dacB"
                     /locus_tag="QBA20_000557"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDQ6291212.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="QBA20_000558"
     CDS             complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="QBA20_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDQ6291213.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41211..42176)
                     /locus_tag="QBA20_000559"
     CDS             complement(41211..42176)
                     /locus_tag="QBA20_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDQ6291214.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="QBA20_000560"
     CDS             complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="QBA20_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDQ6291215.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="QBA20_000561"
     CDS             complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="QBA20_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDQ6291216.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43150..44121
                     /gene="ispB"
                     /locus_tag="QBA20_000562"
     CDS             43150..44121
                     /gene="ispB"
                     /locus_tag="QBA20_000562"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDQ6291217.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44348..44626
                     /gene="sfsB"
                     /locus_tag="QBA20_000563"
     CDS             44348..44626
                     /gene="sfsB"
                     /locus_tag="QBA20_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDQ6291218.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44674..45933)
                     /gene="murA"
                     /locus_tag="QBA20_000564"
     CDS             complement(44674..45933)
                     /gene="murA"
                     /locus_tag="QBA20_000564"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDQ6291219.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="QBA20_000565"
     CDS             complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="QBA20_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDQ6291220.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="QBA20_000566"
     CDS             complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="QBA20_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDQ6291221.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="QBA20_000567"
     CDS             complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="QBA20_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDQ6291222.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="QBA20_000568"
     CDS             complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="QBA20_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDQ6291223.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="QBA20_000569"
     CDS             complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="QBA20_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDQ6291224.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="QBA20_000570"
     CDS             complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="QBA20_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDQ6291225.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49714..50679
                     /locus_tag="QBA20_000571"
     CDS             49714..50679
                     /locus_tag="QBA20_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDQ6291226.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50693..51679
                     /gene="kdsD"
                     /locus_tag="QBA20_000572"
     CDS             50693..51679
                     /gene="kdsD"
                     /locus_tag="QBA20_000572"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDQ6291227.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51700..52266
                     /gene="kdsC"
                     /locus_tag="QBA20_000573"
     CDS             51700..52266
                     /gene="kdsC"
                     /locus_tag="QBA20_000573"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDQ6291228.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52263..52838
                     /gene="lptC"
                     /locus_tag="QBA20_000574"
     CDS             52263..52838
                     /gene="lptC"
                     /locus_tag="QBA20_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDQ6291229.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52807..53364
                     /gene="lptA"
                     /locus_tag="QBA20_000575"
     CDS             52807..53364
                     /gene="lptA"
                     /locus_tag="QBA20_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDQ6291230.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53371..54096
                     /gene="lptB"
                     /locus_tag="QBA20_000576"
     CDS             53371..54096
                     /gene="lptB"
                     /locus_tag="QBA20_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDQ6291231.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54144..55577
                     /gene="rpoN"
                     /locus_tag="QBA20_000577"
     CDS             54144..55577
                     /gene="rpoN"
                     /locus_tag="QBA20_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDQ6291232.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55600..55887
                     /gene="hpf"
                     /locus_tag="QBA20_000578"
     CDS             55600..55887
                     /gene="hpf"
                     /locus_tag="QBA20_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDQ6291233.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56005..56496
                     /gene="ptsN"
                     /locus_tag="QBA20_000579"
     CDS             56005..56496
                     /gene="ptsN"
                     /locus_tag="QBA20_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDQ6291234.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56542..57396
                     /gene="rapZ"
                     /locus_tag="QBA20_000580"
     CDS             56542..57396
                     /gene="rapZ"
                     /locus_tag="QBA20_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDQ6291235.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57393..57665
                     /gene="npr"
                     /locus_tag="QBA20_000581"
     CDS             57393..57665
                     /gene="npr"
                     /locus_tag="QBA20_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDQ6291236.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57878..58510
                     /gene="yrbL"
                     /locus_tag="QBA20_000582"
     CDS             57878..58510
                     /gene="yrbL"
                     /locus_tag="QBA20_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDQ6291237.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58507..59234)
                     /gene="mtgA"
                     /locus_tag="QBA20_000583"
                     /pseudo
     CDS             complement(58507..59234)
                     /gene="mtgA"
                     /locus_tag="QBA20_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59231..59884)
                     /gene="elbB"
                     /locus_tag="QBA20_000584"
     CDS             complement(59231..59884)
                     /gene="elbB"
                     /locus_tag="QBA20_000584"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDQ6291238.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60114..62450)
                     /gene="arcB"
                     /locus_tag="QBA20_000585"
     CDS             complement(60114..62450)
                     /gene="arcB"
                     /locus_tag="QBA20_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDQ6291239.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62546..63475)
                     /locus_tag="QBA20_000586"
     CDS             complement(62546..63475)
                     /locus_tag="QBA20_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDQ6291240.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDVVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64150..68610
                     /gene="gltB"
                     /locus_tag="QBA20_000587"
     CDS             64150..68610
                     /gene="gltB"
                     /locus_tag="QBA20_000587"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDQ6291241.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68623..70041
                     /gene="gltD"
                     /locus_tag="QBA20_000588"
     CDS             68623..70041
                     /gene="gltD"
                     /locus_tag="QBA20_000588"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDQ6291242.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70225..70704
                     /locus_tag="QBA20_000589"
     CDS             70225..70704
                     /locus_tag="QBA20_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDQ6291243.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17317 a        19177 c        17554 g        16662 t
ORIGIN      
        1 ggttaccgcg tagcctggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
       61 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
      121 aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
      181 atggaacctt cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg
      241 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
      301 cttttgcgct tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga
      361 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttat caggaaaacc
      421 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt
      481 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
      541 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
      601 gcaacgatga tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
      661 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
      721 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacag
      781 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
      841 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt
      901 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
      961 ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc
     1021 tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga
     1081 catcagacag gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca
     1141 aacgacattc caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag
     1201 ataaaaagca gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     1261 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     1321 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg
     1381 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     1441 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaagtt
     1501 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     1561 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     1621 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg
     1681 cgaagaagtt cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     1741 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     1801 gtcaatatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     1861 cagggcacag ccaccattac cgtcatggtg cggtaaaaga agacgttacg ttttgattaa
     1921 cgcaacgctt cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt
     1981 cttcacgcca tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg
     2041 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     2101 ttcgacaatc agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag
     2161 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     2221 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     2281 gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     2341 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagatggtaa
     2401 acccgcggcg cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat
     2461 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     2521 ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc tttgccagca gcgtttcggc
     2581 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     2641 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     2701 ctctaacgta cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     2761 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     2821 ttaatattga gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt
     2881 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     2941 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga
     3001 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     3061 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     3121 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc
     3181 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     3241 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     3301 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgcttgg
     3361 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     3421 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     3481 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     3541 cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     3601 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     3661 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagccg cgaaaaatat
     3721 cggcactcag ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagcagaaca
     3781 acctcagccg caaactgcgg atagcgttgc cagcccggca caagcctcgg ttagcgatct
     3841 gaccggtgat cagcctgcgg cccagccggt gcctgtaagc gccccggcga caagcaccgc
     3901 agcggtaagc gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca
     3961 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc
     4021 gttgctgaaa aataacgttg aagagttgct gaagagcaac accccgctga acgtactggc
     4081 actgaaccag ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc
     4141 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     4201 ggtgctgatc ccacgcagtg cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg
     4261 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     4321 cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt agcccgatta ctcccagagc
     4381 gacaaccgac tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     4441 ccagttcacc aataatggcg gtcgtgtcga tgcggtctac attgtggcaa cgccgggtga
     4501 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct
     4561 gtacgccagt tcccgcagcg cacaaggcac cgcaggcccg gatttccgac tggagatgga
     4621 aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt aatctgccgt taatgcagca
     4681 ggcgctcagc gcggtgaaca acgattattc gctggctcgc atgtatgcga tgggcgttga
     4741 tgcctggtcg ttggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     4801 cggtaatacc ggaagtctga cggctaaccc ggattgcgtg attaacagga agttatcatg
     4861 gctacagtac caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat
     4921 gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag gactgcggtt tatcgccgct
     4981 aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc gtgaaggcct gaccaccgtt
     5041 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     5101 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     5161 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     5221 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     5281 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     5341 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     5401 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     5461 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     5521 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     5581 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     5641 cgatattgtt aaagcagttg aagccgccgt tacgcgtgat atgaccattg tggcattgac
     5701 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caggatgtgg agatccgcat
     5761 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     5821 cgatctgatc gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc
     5881 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     5941 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     6001 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     6061 gattaagaaa gaaacccgta ttaatgtcac ggcctatcag ggcaaagtgc tgctggttgg
     6121 gcagtcacca aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     6181 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc
     6241 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     6301 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     6361 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt
     6421 taccacagcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     6481 cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca
     6541 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     6601 cactgccacc atcgcccctg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat
     6661 aatcagtgac ggcaaactca ctgccgggag cgtcatcaac aatgccagcg ccggagcggt
     6721 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     6781 aaacaagcat cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc
     6841 atcggcatgg gggaataacc agacgcgtgc ggcacccaac accagtactg caaggatgta
     6901 aaccgggatc gtactccaga aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc
     6961 gctctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcggtg tttcacgcac
     7021 ccatttttgc accagcgtcg caatcagcaa cgccatcacc agcccggcca ccagacgaat
     7081 cgccgcaaaa ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac
     7141 cggattgccc atccagaatg ccagcgcacc gcccatcgat acctgttggc gacgcattcc
     7201 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc
     7261 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
     7321 cagcacctga atcaacgaac cgaggatcac ccccagcacc gccgctttcc atactgcgag
     7381 gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag
     7441 gatagattta ccgatactgt gggtttcggc tgcagtaaac gctttgccgt agtaaggttg
     7501 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
     7561 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
     7621 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
     7681 agctcttttg cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat
     7741 cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg
     7801 taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc
     7861 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc
     7921 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc
     7981 actgaccacc agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac
     8041 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
     8101 gagacaacaa aacacaatgt cgatgggttc ggtgacctgc gccaacgcgt cactcagttg
     8161 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc
     8221 aatggcgtta actttcggtt cattaatcaa catccgcagc aggtgaccgc ccaccagccc
     8281 cgttgcgcct gtaatcagta cctgactcat cttcgctcct ttacagaatt gtctgccttg
     8341 cgctccccgg ctcatgcacc agtcttaata gaccatgagg taattatccc cgattgtgga
     8401 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
     8461 aatcactgat gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc
     8521 cggacacgaa gtgattacta ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg
     8581 agaagccagc gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc
     8641 cctgttgcta ccgggtggtc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac
     8701 ctttacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca
     8761 gctactgatc agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat
     8821 tattgatgtt aaaaatgcgg gcgtggaatt ttacgatcag gaagtagtgg ttgataaaga
     8881 tcagctggtt accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg
     8941 cctgctcggt gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt
     9001 cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa
     9061 ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc
     9121 ggatctcacc tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta
     9181 ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg
     9241 tcaaaatgta gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga
     9301 gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga
     9361 tggcgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg
     9421 atgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat
     9481 accgggatca ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg
     9541 aaagcactgc gcgggaaagg agaactgacg ctggcgtttt ctgcgccagt gggcgaccgt
     9601 tcgctggcac tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt
     9661 ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac cccggagatt
     9721 aaaagcgatt gaaatgctcg tgatactcaa ccagaccggt tacgccattc agcgcgtcat
     9781 ccgctaaacg atgtaccagg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt
     9841 gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca
     9901 ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca
     9961 gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt
    10021 cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat
    10081 ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg
    10141 cttccgcatc actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg
    10201 gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt
    10261 tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg
    10321 catttgctcc agttcaatgg cgtccatcag gtttttcaca tacaccccca gctccgtatc
    10381 gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc
    10441 aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg tgttctggct
    10501 gaccaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg
    10561 aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa
    10621 ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac
    10681 cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac
    10741 gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc
    10801 ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct
    10861 taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt
    10921 tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc
    10981 ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga
    11041 taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt
    11101 gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc
    11161 ggacggttac gcccgttggc agcgtgccgt ggatatggcg gcgcagctgg gtgccgacgc
    11221 gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc cgcatattga
    11281 acgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg
    11341 ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca
    11401 gctggcacgg gttactcctg tcccgctgga agtgtttgct ttcggcagcc tgtgcattat
    11461 gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acactgtggg
    11521 tgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg
    11581 cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct
    11641 gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag
    11701 tctcaatacc ctggaactgc tgccggagtt aatggctgcg aatattgctt cggtgaaaat
    11761 tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg gcgaaagtct ggcgtcaggc
    11821 tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt ggatggagac
    11881 gctcgggtcg atgtccgaag gcacgcaaac cacccttggc gcgtatcacc gtaaatggca
    11941 gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg
    12001 ctggaagatt tttatcagca agccgccgcc agcagcgccg acgtgattta tcttggtgaa
    12061 gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact gtctggagat ggcaaaatcg
    12121 ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca ggcatcctct
    12181 gaactgggcg aactgaaacg ctatgttgag aacggtgaat ttctgattga agccagcgat
    12241 ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg
    12301 aactgctaca acgcggtgac gctgaaaata ttgctcaaac agggcatgat gcgctggtgt
    12361 atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg
    12421 ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctacc gctggcctac
    12481 tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc
    12541 tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta
    12601 ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc
    12661 atgcagggat tggttgatgt agtccgcctg tcaccgcagg gcactgacac tttcgcgatg
    12721 ctcgacgcct tccgcgctaa tgaaaatggc gctgtgccac tgccgttgac ggcaaacagc
    12781 gactgtaacg gctactggcg gcggctggca ggactggagc tgcaagccta agtaaatagc
    12841 tcactttgtt aacaacttta actattcttt aatgcagtat taaagattaa tcggtaacaa
    12901 agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg gcccccatac
    12961 ccgaaggctc ttcagcgcgg gaagcgttct cccactctct cgatctcgcc cgtctggctg
    13021 aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgacc ggcattgcca
    13081 gtgctgcaac atcggtattg attggctatc tggcggcgaa taccaccacg ctgcatctgg
    13141 ggtctggcgg cgtgatgttg cctaaccact taccgttggt cattgccgaa cagttcggca
    13201 cactcaatac gctctatccg gggcgaatcg atttggggct gggtcgtgct ccgggtagtg
    13261 accaacggac aatgatggcg ctacgtcgtc atatgagcgg cgatattgat aatttccccc
    13321 gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg catgtgcgcc
    13381 cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc ctttacagtg
    13441 cacaactggc ggcgcagctt ggtctgccat ttgcgtttgc ctcacacttc gcgccggata
    13501 tgttgttcca gacgcttcat ctttatcgca gtaacttcaa accgtcggct cggctggaaa
    13561 aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc gacgctgaat
    13621 ttctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa accgggcaac
    13681 tgccgccgcc gattcaaaat atggatcagt tctggtcacc gtctgagcag tatgacgtgc
    13741 agcaggcgct gagtatgtcg ttggtaggcg ataaagcgaa agtgcgtcat ggtttgcagt
    13801 cgatcctgcg cgaaaccgac gctgatgaga ttatggttaa cgggcagatt ttcgaccacc
    13861 aggcgcgcct gcattcgttt gagctggcga tggatgttaa ggaagagttg ttgggatagt
    13921 gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta ggcctgataa
    13981 gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac
    14041 tgatacaccg gcagtaaatt aaagctcgat aaaatatgca ccagtgcgtt gccgacgcca
    14101 aacaccagaa tcagcgcaat catcggcttg ccaccccaga cgcggaattt cgggctgcca
    14161 aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca gatggtagcc
    14221 gctaaaccag cataaccaat ggcatacagg aatccgttcg ggaacagcag accacccaca
    14281 acaggcgggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga gtcgtcgaaa
    14341 ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa cgaactcgct
    14401 accgcaaagt ttgagaacac gaccagcaga aggtccaggc tacggctgtt cagcacgccg
    14461 cttaatgctt gtaccaacac atcaatatta ccgcccttct cggcgatgcc gataaactcc
    14521 ggacgaggga tgttgcccat tgtcgccagc aaccagatgg tatacagcgc cagtgccatc
    14581 agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta atacttcatc
    14641 aggcttggca cgttaccgtg ataaccaaac gacgccaggc agaacggcag ggtcatcagc
    14701 aaatacggcg cataagatgc attgctttcg gcaacgttga acaatgtcgc aggctgcaca
    14761 tgccccagca ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc
    14821 gccgtcatgc ggctgacggc tttagtactc aaccacacca caaacgctac cagcagtgca
    14881 aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa ggtgtgatgc
    14941 agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac aaaggcaatg
    15001 gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa
    15061 ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga atgcagcata
    15121 cagaaccagg taaagatcag cgctgccatt gaccagaaaa accacgcccc ggacatgacc
    15181 actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca
    15241 agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc tctccagtga
    15301 aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacatgagt acaaaagaca
    15361 gaaaaaaagc cccgatggtt aaaatcgggg ctgtatatat tattttacag attgtgttcg
    15421 ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga gtcatcacga
    15481 cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acgaccacct
    15541 tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gctcgccacc gaaaccacga
    15601 ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc
    15661 atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct
    15721 ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccgat gtaacggctg
    15781 ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac gaacttcaac accatcatcg
    15841 cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga
    15901 cgatcttcac ggtcgccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca
    15961 cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt
    16021 tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag ctcttcacct
    16081 tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc gctgctttcc
    16141 agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag cagttctgcg
    16201 ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga
    16261 cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc acgaccggta
    16321 cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt aaccaccagg
    16381 ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga
    16441 cgaccgtctt taaggcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc
    16501 agcgcggcgc tattgtagcc gttacgctca agagcttcag ccacttccag agtcgcgttt
    16561 ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt
    16621 gcttcgtttt tacgcatacc ccacacagtc cagtagctct ggctgatgtc aggacgggta
    16681 gtcacgctgg actgaatacg cacttcctgc ggctctttca taaagcggcg ggtaatgcga
    16741 cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc
    16801 gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc
    16861 agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga
    16921 cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca
    16981 taacgctggc cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt agagaaatcc
    17041 gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt
    17101 ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt
    17161 ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac
    17221 tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga
    17281 gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc
    17341 aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga
    17401 aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag
    17461 gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt
    17521 ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct
    17581 caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca
    17641 aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag
    17701 cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa
    17761 tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga
    17821 agtgctgttt caatacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa
    17881 gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt
    17941 gcgctaactt gtcacgaccg ccgtaatata atgcaatccc gcgattcaag tgcgcgtagt
    18001 tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc
    18061 ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg
    18121 ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca
    18181 ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga
    18241 tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga
    18301 ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa
    18361 cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac
    18421 gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg
    18481 agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag
    18541 gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt
    18601 ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc
    18661 gtttgtcagc gatttgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa
    18721 atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc acttcgattt
    18781 ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg
    18841 caatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcaggg
    18901 cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga
    18961 tggtatggat acgtggtgcg aactcagaga tatcgccacg cggcgcgttg atcgcctgtt
    19021 ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga
    19081 tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata ccctcgcggg
    19141 aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca
    19201 gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt
    19261 tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag
    19321 aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg aatttgtcca
    19381 tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac
    19441 gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga
    19501 acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgagcag
    19561 tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt
    19621 gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct ttttcacgac
    19681 cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatcgcgt
    19741 gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga
    19801 tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt
    19861 aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta
    19921 ccggctccgg ctgccagtcc caacgtggtt taccggcttc tttcaccagt tcattgatgt
    19981 tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc atctggtctt
    20041 cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta ccggcaacaa
    20101 ccagatccag tttgctctct ttcaactcgt cctgagtcgg gttcagtacg tactggtcat
    20161 tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa cggaatacca gacagagaca
    20221 gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa
    20281 ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc ggccgaatcg
    20341 ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga
    20401 agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg
    20461 ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca aataccgcgg
    20521 tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca
    20581 gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat
    20641 cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca
    20701 tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg
    20761 cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg
    20821 ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt
    20881 agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca
    20941 ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt
    21001 gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg
    21061 ctacctgaac ttcagtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt
    21121 tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga
    21181 cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc
    21241 gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac
    21301 gccgggtatt caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc
    21361 ataccgataa atttgccgtt ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca
    21421 ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa cggaagattc
    21481 accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc
    21541 gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg ttccagggtc
    21601 acaatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat aacatgcgcg
    21661 ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag
    21721 cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg
    21781 gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc gtacagtttt
    21841 ttaccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa
    21901 gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc ttcaacgatt
    21961 tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt
    22021 ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac
    22081 atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc
    22141 cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc cagcaacaaa
    22201 acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt
    22261 ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc
    22321 gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc
    22381 gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac gctttgatgc
    22441 ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg
    22501 ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg cgaggatctt
    22561 taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg
    22621 ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct
    22681 ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg
    22741 tcacatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa
    22801 aaaccttaag cgatggtacg ctggatctcg atgatttcga atacttcgat cacatcgcca
    22861 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    22921 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg
    22981 tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct
    23041 gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc
    23101 tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcaatcagg
    23161 ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca
    23221 gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc agccagggtg
    23281 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    23341 gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac gtctgccttc
    23401 aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    23461 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atacagtgca
    23521 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggtacg
    23581 ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc
    23641 agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    23701 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    23761 aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact
    23821 tccgcctgca gcaggatagc gtccagcagt tcatcgatac cagtacccgc tttcgcagac
    23881 acatgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    23941 tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaacgacc
    24001 accggaacct gcgccgcttt cgcgtgctgg attgcttcga tcgtctgcgg cattacgccg
    24061 tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt
    24121 gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga tcatgccgtt ttcagtttca
    24181 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    24241 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    24301 accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc
    24361 agctcgtttt cacgacgcag gataacttta tggcccatct cttcagcaac cagctgtgcg
    24421 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg
    24481 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg
    24541 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    24601 gaacctttac gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    24661 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca
    24721 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    24781 gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg
    24841 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    24901 cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc acggcgggct
    24961 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    25021 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    25081 tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct
    25141 tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg
    25201 acttcgattt gtaccgattt gctttttcca ccggtaccag gaatgttaag ggtgctgcgt
    25261 gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt tctgattcag gtggtcaatc
    25321 aaagtctgtt tctcttgtgc agacacagag tcgtcagcag acttccggat acctgcatca
    25381 gcaaattgct gtaccaggcg ttccacggag gtctgtcgct ctgcggccag cgttttaatc
    25441 gttacatctg tcatgctgtt ccttcctgct acagtttatt acgcttcgtc accgaaccag
    25501 caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa cccttcgata
    25561 tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac gccacgggcg
    25621 gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc gtcagccggt
    25681 ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc atttttagca
    25741 cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa cagctctttc
    25801 atcggcacat aggccagttc ttccagcgtc gagaaacctt cttctaccag aacagtcgcg
    25861 aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc
    25921 tgatgcttag cctgcaggtc gtcaacggtc attacgttga gttcccaacc gctcagctgc
    25981 gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccaggtt accagcttca
    26041 acggcgatat ccatggtgtg tttatcttca tccaccacga tagaagcaac gtctgccggt
    26101 gccattgcgt taatcacaaa ctgcgccggg ttatcatccc acaggacgat atcgatacgc
    26161 tcgccgccca gttcagtaga aaccgcctga acacgcgcgc cacgcatacc tacgcaagca
    26221 cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg agaacccggg
    26281 tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttccggcac ttcaatacgg
    26341 aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc gccacgcgct
    26401 tccgggcgaa cggaataaag cacgccacga acgcggtcgc cagggcggaa gttttcacgc
    26461 ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc cagagagatg
    26521 ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg ttcacggaac
    26581 tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac ctgttttgcc
    26641 gtctgggtgg tgatacggtc aaaggtaaca gactcaatct gatcttcaac gtaatcgccc
    26701 aagttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg
    26761 gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg
    26821 atctgtacac ggacgtcgat ctcttgctca tattttttct ttgttgctgt cgccagcgcg
    26881 ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga tacggcttca
    26941 actacagcca aaatttcttt gttcatcgcg ggcttttcac ctcatccaga ctattaaaag
    27001 tggggaacca ggttcgcctt ctggatatta ctcagcgcga acacttcatc tttaccttcg
    27061 acggtaactg tgatcatttc accgtctacc gctttgataa cgccctgcca tttacgacgg
    27121 ttttgtaccg ccatacggag aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt
    27181 tcagccgtga acagtgggcg atcgagaccc ggtgaggaga cttccaggtt ataagcaacg
    27241 gtgatgggat cttcaacatc cagcacagca cttacctggt ggctcacatc agcacaatca
    27301 tcaacattga tgccatcttc actatcaata tagatgcgca gtgtggatgt gcgaccgcga
    27361 ataaattcga tgcctaccag ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct
    27421 gttaattttt gctctaatgt ggacaagccc acccccaaga cataaaaaaa gggcctaaag
    27481 cccagttatt ctgtagtcag ataacaaaaa accccgataa atcggggctt tatataactg
    27541 aaccctataa ccgcaactgc ggtctggagc actttccaga aggatttttt caaatcccac
    27601 tacgaaggcc gaagtcttca cagtatattt gaaaaaggac tctaagggaa agtggttgcg
    27661 ggggccggat ttgaaccgac gaccttcggg ttatgagccc gacgagctac caggctgctc
    27721 caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc
    27781 tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca
    27841 cattttgtca acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt
    27901 gaatactttt gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa
    27961 actcttcctg tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat
    28021 tcagtgagag cggaaatcca agctcatcat cagttaatta agcagggtgt tattttatga
    28081 cgacgattct caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc
    28141 tggacaccag tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata
    28201 ctgcaaacct gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg
    28261 aatacggcgc ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta
    28321 ttgccgctat tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca
    28381 cgacgccgct gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg
    28441 gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc
    28501 gttatggtct gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact
    28561 ttattgatga actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg
    28621 actacaaaat gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc
    28681 atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg
    28741 gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg
    28801 agcaaggtca tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc
    28861 tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc
    28921 gtatcatcga agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca
    28981 ttgcgtatga acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg
    29041 cgcatggtcg tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc
    29101 tgatgctgcg tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc
    29161 tggagctgcg ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct
    29221 acaagccaga gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta
    29281 ttggtcaatt gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt
    29341 atgccaaaac tggcctgctt tcctcctctg ccacttcagg cctgccgcag gtggagaata
    29401 tggaaaacaa aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg
    29461 cttatcctct gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc
    29521 cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc
    29581 cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat
    29641 attgttttcc gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat
    29701 cgggacatga tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt
    29761 cgtagggtca cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt
    29821 taataattca aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca
    29881 ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc
    29941 cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg
    30001 taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc
    30061 catggcgcgc atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga
    30121 gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta
    30181 attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc
    30241 agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc
    30301 aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga
    30361 ctcccccaca atcaacacgt aggtatcaat acttgtatcc ctgactgata attgaaaata
    30421 cggcaccgtg tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata
    30481 gttgagattg aaaaacggcg tataggtagc aaatcacgac gctagtatat atggactgaa
    30541 cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
    30601 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
    30661 tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat aagggatata
    30721 catccccagc attttcacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc
    30781 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
    30841 agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt
    30901 aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg ctccggaaga
    30961 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
    31021 aaaatcttct agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata
    31081 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
    31141 tcagaccgaa acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta
    31201 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    31261 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    31321 ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc
    31381 gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga
    31441 tacctgacaa ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc
    31501 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    31561 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    31621 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt
    31681 gcactcagat tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc
    31741 accagactca tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    31801 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    31861 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgt cacaataagg
    31921 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    31981 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    32041 taaccaaaac gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    32101 aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca
    32161 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    32221 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    32281 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    32341 ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct
    32401 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    32461 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
    32521 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    32581 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
    32641 caagccccat gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    32701 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    32761 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    32821 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    32881 caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    32941 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    33001 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga
    33061 tacggctggc tttacccagt tctgccgaat caacgcagct gatctccttt tccatttccg
    33121 cttcgatggc ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa
    33181 tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    33241 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    33301 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    33361 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    33421 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    33481 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    33541 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    33601 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    33661 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    33721 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    33781 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    33841 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    33901 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    33961 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga
    34021 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
    34081 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    34141 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    34201 ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca
    34261 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    34321 tccagtgaag taccctgggc aaagagtttc attatgttat ccctggtatg aattgataag
    34381 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    34441 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    34501 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    34561 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    34621 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    34681 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    34741 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    34801 atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    34861 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    34921 aaacatgttc cgacccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    34981 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    35041 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg
    35101 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg
    35161 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    35221 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    35281 tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg
    35341 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    35401 cgcgaacatc tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg
    35461 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    35521 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    35581 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    35641 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    35701 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    35761 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    35821 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    35881 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    35941 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    36001 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    36061 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    36121 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    36181 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    36241 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    36301 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    36361 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    36421 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    36481 gtttacgccc ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct
    36541 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    36601 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    36661 ctagcgccag ttccaccaga tacatggctc gggggatatc caccgccggt gttccgctca
    36721 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    36781 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    36841 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    36901 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    36961 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct
    37021 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    37081 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    37141 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    37201 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    37261 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    37321 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    37381 atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    37441 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    37501 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    37561 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    37621 gcagagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    37681 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    37741 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    37801 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    37861 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    37921 ttggtgacat caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct
    37981 tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    38041 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    38101 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    38161 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    38221 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    38281 ggtcagctac aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg
    38341 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    38401 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    38461 gccgcgaatg ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg
    38521 gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    38581 cccgccagta cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    38641 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    38701 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    38761 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    38821 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    38881 tttagcgctc cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac
    38941 agtgccccaa agcctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    39001 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg
    39061 gatgtggtgc caagcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    39121 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg
    39181 aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag
    39241 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt
    39301 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgatcggc
    39361 catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc
    39421 ctgcgccagc aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt
    39481 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    39541 cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg
    39601 cagtaccgtg caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt
    39661 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    39721 gcatttgtac aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt
    39781 attccgttag tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca
    39841 aaaagaaacc ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat
    39901 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc
    39961 aatcatcttg taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt
    40021 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    40081 cagtcttcat catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac
    40141 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    40201 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc
    40261 agataatatt tatcttccca gcccagcgct tcagcgatcg ctttcgcttt ctcttcggct
    40321 tctgccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    40381 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    40441 ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    40501 aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg
    40561 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    40621 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    40681 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    40741 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    40801 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    40861 ccgtgtttag tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct
    40921 accggcactt taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg
    40981 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    41041 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    41101 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    41161 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    41221 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    41281 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    41341 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    41401 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    41461 gcttccgcca aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    41521 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt
    41581 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    41641 ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc
    41701 caggtgtaat cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc
    41761 agaccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    41821 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    41881 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg
    41941 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    42001 ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac
    42061 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    42121 cagcaaattg ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    42181 tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat
    42241 cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa
    42301 acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc
    42361 actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca
    42421 gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca
    42481 ccgaaacgct taacgcccaa gcgtttagct tctgaatcgc gaccgttacg tgtggagccg
    42541 ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa
    42601 ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac
    42661 gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta
    42721 cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca
    42781 cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct
    42841 gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact
    42901 ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc
    42961 gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa
    43021 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatt accctatatt
    43081 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    43141 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    43201 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    43261 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    43321 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    43381 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    43441 accgccaacg ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    43501 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    43561 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    43621 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    43681 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    43741 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    43801 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    43861 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    43921 gccatcgaac aaggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    43981 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    44041 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    44101 gctgttcaac gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc
    44161 agtaaattcc ataaaaacac taattcagcg ctataacacc tgaaaaaagg gcgatgaatc
    44221 tttatatatt ctgaatattc acactcttta caggaacttt ttagcgcaat agaccatcag
    44281 gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg
    44341 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    44401 caagaaagga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct
    44461 ggcaaacgca ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg
    44521 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    44581 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    44641 gccccgcgaa cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt
    44701 tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    44761 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    44821 acgcagatcg gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac
    44881 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    44941 gcggttttca aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag
    45001 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    45061 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    45121 cgctccagca tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    45181 gcagataatt ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    45241 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    45301 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    45361 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    45421 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    45481 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    45541 ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg
    45601 tagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    45661 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    45721 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt
    45781 atcgacgtct ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    45841 ggcaaaaaga ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    45901 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    45961 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    46021 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    46081 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc
    46141 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    46201 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    46261 ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    46321 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    46381 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    46441 cgcgagggta tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt
    46501 cgccagatcg ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg
    46561 gctaagatcg atgcaggtaa tccccttcac cgcctcctca cgcatttccc aaagcggtag
    46621 cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    46681 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    46741 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    46801 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc
    46861 tgggagtttt tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata
    46921 atggtaacgc gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga
    46981 taggtttgac cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg
    47041 aaagcggcaa agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc
    47101 accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta
    47161 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    47221 ttctgcgccg cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca
    47281 ctcagaggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    47341 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    47401 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    47461 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    47521 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc
    47581 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    47641 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    47701 cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta
    47761 tcgaacgtcg cataaagcgt gtaggtcgat tcagtacgta tggacgtcac gttcgccgcc
    47821 ttcaggcaaa caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt
    47881 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    47941 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    48001 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    48061 gaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg
    48121 cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc
    48181 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc
    48241 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    48301 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc
    48361 ggcaaacaac agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    48421 catacccaga ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa
    48481 caccattccg atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt
    48541 atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc
    48601 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    48661 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    48721 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    48781 gacgtacgcg cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt
    48841 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    48901 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta
    48961 cgcccatggt gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct
    49021 ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    49081 gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    49141 gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata
    49201 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg
    49261 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat
    49321 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    49381 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    49441 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    49501 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    49561 acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac
    49621 tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg
    49681 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    49741 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    49801 tgccgaacct ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca
    49861 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc
    49921 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    49981 ctggttcgtc cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg
    50041 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt
    50101 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    50161 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    50221 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    50281 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatggctctg
    50341 acggcaattg ctatcggaac cagtctgccg gaactggcaa ccgcaatagc gggggttcgc
    50401 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    50461 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    50521 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    50581 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    50641 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    50701 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    50761 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    50821 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    50881 cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    50941 tagtgaagcc gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    51001 ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca
    51061 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    51121 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    51181 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    51241 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    51301 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    51361 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    51421 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    51481 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    51541 tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    51601 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    51661 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    51721 cttgcgacct gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt
    51781 ctgctgatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    51841 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    51901 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    51961 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    52021 gatttgctgg aaaaattggc gattgccccg gaaaatgtgg cttatgttgg cgatgatctc
    52081 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    52141 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc
    52201 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    52261 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    52321 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    52381 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    52441 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    52501 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    52561 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    52621 ttgtgccaga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    52681 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    52741 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    52801 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    52861 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    52921 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    52981 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc
    53041 ggcgaacaag gtaaagaagt gattgacgga tacggtaaac cggcaacgtt ctaccagatg
    53101 caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    53161 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc
    53221 gataagatca cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    53281 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc
    53341 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    53401 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    53461 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta
    53521 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    53581 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    53641 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    53701 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    53761 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    53821 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    53881 atctcggtta tcgacattaa acgcatcatt gaacacctgc gcgacagcgg cctgggcgtg
    53941 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    54001 agtcaggggc atttgatcgc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    54061 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    54121 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    54181 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    54241 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    54301 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    54361 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    54421 ccgctggtac accatccggc accagcggtg actacattga cgacgagctg ccggtctacc
    54481 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    54541 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gaaaccggtt
    54601 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    54661 acgaggttga agccgtcctt aagcggatcc aacggtttga cccggtcggt gtagcggcaa
    54721 aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataagacc acgccgtggc
    54781 tggaagaggc cagattgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    54841 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    54901 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    54961 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    55021 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg
    55081 atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    55141 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    55201 ccttctttga gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    55261 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    55321 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    55381 gcgaagcttc ctccacggcg attcgtgcac tggtgaagaa attaatcgcg gcggaaaacc
    55441 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    55501 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    55561 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    55621 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagctaaatt tgccaaactt
    55681 gagcaatatt ttgaccgaat caaccaagtc tatgttgttc tgaaagtgga gaaagtcacc
    55741 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    55801 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    55861 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    55921 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    55981 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    56041 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    56101 gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa
    56161 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    56221 catggcaaac ttgaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg
    56281 cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg
    56341 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg
    56401 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    56461 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    56521 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    56581 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    56641 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc
    56701 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    56761 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    56821 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    56881 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    56941 gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg
    57001 ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    57061 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    57121 aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    57181 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    57241 gaaaccaaca accgtagcta cctgacggtc gccattggtt gtaccggcgg gaagcaccgt
    57301 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    57361 tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    57421 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    57481 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta
    57541 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc
    57601 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taattctggt tttgatgaag
    57661 attaatcttc atcactttga catacaactc ccttcaagct cccccgacaa taagaaaatc
    57721 acgtactgaa atcgttctca atcaacgtca tttgtacatt ttgtgcgctt ttcactttca
    57781 gaagaacctt aagaaaacct taagaggcat tgtttaggtt ttgtttaagt taatcgacca
    57841 tactggagat cgtcagaaaa tatttccagg agatggcatg attcgcttat ctgaacaaag
    57901 tcccctgggc accggaagac atcggaaatg ttatgcgcat ccggaagatg cccaacgctg
    57961 tatcaagatt gtctaccatc gtggcgatgg tggcgataaa gagatccgcc gtgagttaaa
    58021 gtactacgcg catcttggtc gccggttaaa agactggagt ggaataccgc gctatcacgg
    58081 taccgttgag acggattgcg gaaccggtta tgtctacgat gtgattgccg attttgacgg
    58141 caagccttcc attacgctga ccgaatttgc tgaacaatgc cgttacgaag aagacattgc
    58201 acaactgcgc cagctactga aacagctgaa gcgttatttg caggataacc gtatcgtgac
    58261 gatgtcgctg aagccgcaga atatcctctg tcaccgcatc agtgagtcag aggttatccc
    58321 ggtcgtctgc gacaacattg gtgaaagcac attgattcca ctggcgacct ggtcgaagtg
    58381 gtgttgtctg cgtaagcaag agcggttatg gaaacgattt atcgcccaac cggcgctggc
    58441 tattgccctg cagaaagatc tgcaacctcg tgagagcaaa acgctggccc tcacttcgcg
    58501 cgaagcttaa tccagctggt gttgttgcat aaacggctca ccgcctaact gatacatctg
    58561 ccgtaaaatc cacgcctgac ggctacgcac gtagcccgat ggcgaggaga ctttgaaacg
    58621 aagtggatta ggtaatacag ctgccagtaa tgcagcttcc gaccgggtaa gtttgctcgc
    58681 gggtttgtgg aaataacgtt gtgccgcagc ttcgacgcca aacacaccgt cgccaaattc
    58741 ggcgatattc aggtaaaccg tcaggatacg ctttttgctc cagactgttt ctatccccag
    58801 cgttaatcca gcttccagcc cttttcgtac ccagctacgc ccatcccata aaaagagatt
    58861 ttggccgtct gctgagaaat cgttgaagca ccgcgaatac ggttttcatt gcgctcgttg
    58921 tgcgccaggg ctttctcaat ggaagcgaca tcaaagcccc agtgctcagg aaatttctga
    58981 tcttctgcgg caatcaccgc cagtcccatc cacggcgaga tttgatccat actgacccag
    59041 tcagaatgtg ccacgtaacg aaaattgcca tgcagccagg cgctgacctg tcgctcgacc
    59101 attaccgctg agaagggaac aggcgcaacg ctaaacaacg cgatgccccc gccccagaaa
    59161 acggcgagga caaccattaa ccgcaaaaga aaacggcgaa cgaaactaaa caccgttaag
    59221 cggcttttac tcattcagcc agaaccagca cgcgggaaac cagcttatca atgccgctcg
    59281 ccgcttctgc aatgttctgc gccagcatat atgctggggt ggtgacaatc ttattgtctt
    59341 catcaaccac gatatcatcg acaggacacg gcacatgctc cgcgcccatc tcttccagca
    59401 cttctgcggt atcgatatca gtaccgatgg tcaaacgcag cgggaaatcg aaaattttcg
    59461 gcagcatcgc cggggcaata cacataaaac caagcggttt tccggcctga tgcatcgctt
    59521 gtgccagcgc ctttaattca cggtcaacgg tgcattcgct accaagactg gcaaaattgc
    59581 ttaaattctt cgccgcgcca aacccccccg gcacaatcaa cgcatccagt tcagcggcat
    59641 cggcctgggc cagaggacgg atttcaccgc gcgttattcg tgccgcttca atcagcacat
    59701 tgcgcgtttc cgtcatcgct tcgccagtta aatggttgat aacatcaacc tgctgcttat
    59761 ccggtgcaaa gcagaccgcc tgcgcaccgc tgcgtgaaat agctaacaac gtcaacaccg
    59821 cttcatgaat ttcagaaccg tcatagacgc cgcatccgct cagaattacg ccaattttct
    59881 tcattgtgat catcctttcg caattgactg aaacacatta atttttttaa taaaaatggt
    59941 acgcatcaca catttaactg attcatgtaa caaatcattt aagttttgct atcttaactg
    60001 cgtgcggcct gaaaaacagt gctgtgccct tgtaactcat cataataatt tacggcgcag
    60061 ccaagatttc cctggtgttg gcgcagtatt cgcgcacccc ggtctagccg gggtcatttt
    60121 ttagtggctt ttgccaccca cgctttcagc acttctacgt cgtgacgcca ctcttctttc
    60181 atctcttcaa tccattcacc gacgttatct tcccaggcgg gaaggtcagg agactgaatt
    60241 tgctgaccca gctgttgcag atggcgtaac cccactgacc ccgccgcacc tttaatttta
    60301 tgtccttcct caacaatgcc ttttttatcc tgcgccgtca gattcgactc cagcacgctg
    60361 acatagcccg gcatcatctt ctcaaacacc gctaacccgt cggtgatcag cttcggtcct
    60421 acaagttcga gatactgttc cagcatggga atatcgagca atgcttctga tttactgttc
    60481 tcttctgtcg tcaccgtact ctcctcatca tcctgggtat cccagaattt cttgatcatc
    60541 gcggttagcg ccggaaccga aagcggctta ctcagcacat catccattcc agcattgagg
    60601 tactcttgtt tgtctttcag cacgttagcg gttaaggcca ccagcggcgg taaatcctcg
    60661 cgcggataac gcttcgtcag ttcacgagag atatccagcc cggtcatatc tggcaactga
    60721 atatccagca acaccaggtc gtattcgccc ggtttaaaca tctccagcgc cgccttgccg
    60781 gtcatggcga catcaacgct gttacctaat ttttccagca cagaacgcgc gacaatcacg
    60841 ttcagttcaa tgtcttccac cagcagcaca ttcagcgccg gtaaaggcat atcgtcttca
    60901 tcaaacgcat catcgacctc ttctgccacc gacggtgcgt ggatcgtcaa cgtaaaggtt
    60961 gaacctttgc cctgttcact ggtaaccgta atatcgccgc ccatattttt cgccagacga
    61021 cgagaaacgg ccagaccaat accggtgccg gtggcaggtt taccgccatg actgtctttc
    61081 acctggtaat acatggcgaa gattttatcc agctcatcct gcggaatgcc gataccagaa
    61141 tcttccactt caaaatgcag catatcgcct tcatcgtagc gcacgcgcac ggtaacctgg
    61201 ccttgctggg tgaatttgac ggcgttactg atgaggttcc acaggatctg ccgtaaacgc
    61261 gtcccgtcgg taatgacctg atgcggtaat ggcagcgtag gctccaggtt aaagcgcaat
    61321 cctttttgtt gcgcctgcaa tgcggagaga ttttccagat cggcaaggaa gctggtgaaa
    61381 tcaaccggct gattatcaag ctggaccttg cgccgttcca tcttatccat gtcgataata
    61441 tcgttaaaga tattccccag cgtgacggcc gaaacatgga tagttttgag atatttttcc
    61501 tgctcggcgg tgagttcggt atccagcaga atgcggctca ggccgacgat accgttcagc
    61561 ggtgtacgca attcgtgact gatggtggaa ataaacgtcg ttttgtcgcg gctggcgcgt
    61621 tcaagcgcat cctgataccg cttacgctcg gtaatgtcgc gaccaaagcc catcaaaccg
    61681 tgacgtttac ccacgcggtc gtagtacggc actttacgga tttcaaagca ggctttgcgc
    61741 ccgtccgggt aatccagcca ctgttcatag gtcagtgaca cattatgacg gaacactttt
    61801 tcatcggttt caatgacttt tgcggcggct tccggtgagt aaacatcagc aggtttcagg
    61861 tgaaccagtt gtttttcgct ttttccggtc agcagctcca tcgcgcggtt acagccggaa
    61921 aactctttat cttcgttacg ataaaaaacc aagtcgggtg aagcatcaag gaaggaacgt
    61981 aagaatgagg attgctgctc gagctgaatt tgtgtctctt cgcgctcttt gatttcaatt
    62041 ttcagttggc cgaaggtttc ctgcagttct gcttccgctt tttcacgaac ggcaatttcc
    62101 tgatttagct gggcaatatt atcttttaac tgaacgttga ggctcaaatc gcgctcgcgc
    62161 atctcctcca gtttttgcac cagccgtgac agacgttgtc gtgactcctc cagttgctcg
    62221 acgaccaccg atagaaagta gaccgcccac ggcgtaatca gcaaaccaaa gaagatagaa
    62281 cgaataacat caatgctttc gacctgacca tgcagcacca tggttaccgc catttgtacc
    62341 acaatggcaa gaacgacgag ggccagcgcc agcaacattg agaagcgcac cagacctaac
    62401 ttcatcatca ggtcaacata atactgcgcc agcagacgaa tttgcttcat tagggaattc
    62461 cttcacgaca acctgcccca ataataccca attctgacag ttacgttata aattgtgtga
    62521 gaaatgcgga attcccctca acgacctact ccgttggagg tagccacgga cgtcccagcg
    62581 ccgatccctg tacgccatgt tcattcagat agcgatccag ctcgaccatt cccgtccatc
    62641 gattttcgca ccacagcgga gcaagcagcg tcgggcgacg ggcgctggcg gaaatgcggt
    62701 ggtagatcac ttccggtggc gtatggcgaa tcatttctcc ggcagtgagc gtataatcct
    62761 ccagttcaat accgttcaaa cgccccgctt cccaggcttt cgccataatg ctgcctttca
    62821 caatatgcag cggatgcagc tttatgccgt ccacgctggt ttcaaccact cgctccagcg
    62881 tttgcaaaca ttcagcctgc ccttcgccag gcaggccgac aattaagtgg gaacacacct
    62941 tcagcccgcg ctgacgtgcc agctgggttg tacgctgata acaggcaaaa tcatggccgc
    63001 ggttgatgcg atgcagtgtt ttgtcgtggg cggtttgtag ccccagctcc agccacactt
    63061 cgtaaccctg gtccttatat tcgcaaagca gatccagcac cacatccggc acgcagtccg
    63121 ggcgggtacc aacacacaaa ccgacaatat tggcctggct caccgcctgc tgatacatag
    63181 aacgcagcac ctgaacttcc gcaaaggtgc tggtatacgc ctgaaagtag gccagatagc
    63241 gtttagcgcg gttaactaaa ttcgcctggt gcgccagttg ctcggcaatg gaacgatgct
    63301 gctgcgcttc atcggcaaac gaggcaacat tacagaatgt gcagccacca cgcccgatgg
    63361 taccgtcacg gttagggcag ctaaaaccgc cgtgcagcgt cagcttatgc accttttgcc
    63421 cataacgacg ggtgagatca ccaccaaaca tattgactaa tttctgtaac tgcataatct
    63481 gatagaccgc gccttgaaaa gaggccaaag cctgccattt ttagctcaat tcggcgatga
    63541 cctgggtcaa tcgtcccggc ctgcttttat cagctgcata atcaatcaaa attaccgaaa
    63601 tttcatgcac aatcacataa atcacttttg cttatcttgt gtcagatttt tttatctcct
    63661 tatggatttt aggcaaaaac agtagcatga aacgtcatta ccaattaagg cagtataaaa
    63721 tgctggtttt ctcgtcagtt caaggcagga taagggttaa cacgccttta tgacagtcag
    63781 gaattgactg tttctctaac gactctcctt ttagccttaa agataaaatc tattttaatt
    63841 tcagctagtt aataaagaat tttgcgctaa agcacatttc tgtaccaata agcttgccat
    63901 ttgacctgta tcagctttcc cgataagttg gaaatccgct ggaagctttc tggatgagca
    63961 gcctgctcat catatttatg cagtaattga gatcccctct tcaccgtatt aaccgatgcg
    64021 aaaaggacaa caagggggcg aatgcgaggc gcgcgtatga cacgcaaacc ccgtcgccac
    64081 gctctttctg tgcccgtgcg cagcggttcg gaagtggggt tcccgcagag cctgggggag
    64141 gttcacgata tgttgtacga taaatccctt gagagggata actgtggttt cggcctgatc
    64201 gcccacatag aaggcgaacc tagccacaag gtagtgcgta ctgcaataca cgcactggcc
    64261 cgcatgcagc accgtggcgc gattctcgcc gatggtaaaa ccggcgacgg ttgcggcttg
    64321 ctgttacaaa aaccggatcg cttttttcgc atcgttgcgc aggagcgcgg ctggcgttta
    64381 gcaaaaaact acgctgtcgg gatgctcttc ctgaataaag atcctgaact cgccgctgcc
    64441 gcacgccgca tcgttgaaga agaactgcaa cgcgaaacct tgtcgattgt gggctggcgt
    64501 gatgtcccca ctaacgaagg cgtgctgggt gaaatcgccc tctcctctct gccacgcatt
    64561 gagcaaattt ttgtgaacgc cccggcaggc tggcgtccac gcgatatgga gcgccgtctg
    64621 tttatcgccc gccgccgcat tgaaaagcgt ctcgaagccg acaaagactt ctacgtctgt
    64681 agcctgtcga atctggtgaa catctataaa ggtctgtgta tgccggcgga tctgccgcgc
    64741 ttttatctgg atcttgcgga cctgcgtctg gaatcggcca tttgcctgtt ccaccagcgc
    64801 ttctccacta acaccgtacc gcgctggccg ctggcgcaac cgttccgcta tctggcgcat
    64861 aacggtgaaa tcaacaccat caccggtaac cgccaatggg cgcgtgcgcg tacctataaa
    64921 ttccagacgc cgctgatccc tgacctgcac gacgccgcgc cattcgtcaa cgaaaccggt
    64981 tctgactcca gttccatgga taacatgctg gaactgctgc tggcaggcgg gatggatatc
    65041 atccgcgcca tgcgcctgtt agtgccaccc gcctggcaga acaacccgga tatggacccg
    65101 gaactgcgtg ccttctttga ctttaactcc atgcatatgg agccgtggga tggtccggcg
    65161 ggcatcgtga tgtccgatgg tcgcttcgct gcctgtaacc tcgaccgtaa cggtctgcgt
    65221 ccggcacgct acgtcattac caaagacaag ctcatcacct gcgcctctga agtcggtatc
    65281 tgggattacc agcctgacga agtggtcgaa aaaggccgcg tcgggccagg cgaactgatg
    65341 gtgatcgaca ctcgcagtgg gcgtattctg cactcggcag aaaccgatga cgatctgaaa
    65401 agccgccacc cttataaaga gtggatggag aaaaacgtcc gccgactggt accgtttgaa
    65461 gatctgcccg atgaagaagt gggtagccgc gaactggacg acgacacgct tgccagctac
    65521 cagaaacagt ttaactacag cgcggaagag ctggactccg taattcgcgt actgggcgaa
    65581 aacggtcagg aagcggtcgg ttcgatgggc gatgataccc cattcgccgt gctctccagt
    65641 cagccgcgca ttatttacga ctacttccgc cagcagtttg cccaggtgac taacccgcca
    65701 atcgacccgc tgcgtgaagc gcatgttatg tcgctcgcca ccagtatcgg tcgtgaaatg
    65761 aacgtctttt gcgaagcaga gggccaggcg caccgtttaa gctttaaatc gccgattctg
    65821 ctctactccg atttcaaaca gctcacgacg atgaaagagg agcactaccg cgcagatacg
    65881 ctggatatca cctttgacgt cactaaaacc acgctcgaag cgacagtcaa agagctatgc
    65941 gacaaagccg aaaaaatggt acgtagcggc accgtgttgc tggtgctctc cgaccggaat
    66001 atcgctaaag atcgcctgcc ggttccggct ccgatggcgg ttggcgcgat ccagacccgt
    66061 ctggtcgatc aaagcctgcg ttgcgatgcc aacatcatcg tcgaaaccgc cagcgcccgc
    66121 gatccgcacc acttcgccgt gttgctgggt ttcggcgcga cggctattta tccgtacctt
    66181 gcctatgaaa cgctgggccg cctggtagac acccacgcga ttgccaaaga ttatcgtacc
    66241 gtgatgctca actaccgtaa cggcatcaac aaaggcttgt acaaaatcat gtccaaaatg
    66301 ggcatctcca ccatcgcctc ttaccgctgc tcgaaactgt ttgaagcggt cggtctacac
    66361 gatgatgtag tgggcctgtg cttccagggc gcggtcagcc gcattggtgg ggcaagcttt
    66421 gaagacttcc agcaggatct gctgaatctg tcgaaacgtg cctggctggc gcgcaagccg
    66481 attagccagg gcggcctgct gaaatacgtc cacggcggcg aataccacgc ctacaacccg
    66541 gacgtggtgc gcacgctgca acaggcggta caaagcggcg agtacagcga ctatcaggaa
    66601 tacgcgaagc tggttaatga gcgtccggca accacgctgc gcgatctgct ggcaattacg
    66661 ccgggtgaaa acgcggtcaa cattgctgat gttgaaccgg caagcgaact gtttaaacgc
    66721 tttgataccg ccgcgatgtc tatcggcgcg ttaagcccgg aagcccacga ggcgctggcg
    66781 gaagcgatga atagcatcgg cggtaactcg aactccggtg aaggcggcga agacccggcg
    66841 cgctatggca ctaacaaagt gtcgcgcatc aagcaggtgg cttctggtcg cttcggcgtt
    66901 actccggcgt atctggtcaa tgccgacgtc attcagatta aagtcgccca gggcgcgaag
    66961 ccgggcgaag gtggtcagtt gccgggcgac aaagtcactc cttacatcgc caaactgcgc
    67021 tattcggtgc ccggagtgac gctgatctcc ccgccgccgc accacgatat ctactctatc
    67081 gaagacttag cgcagcttat tttcgacctc aagcaggtta acccgaaagc gatgatctcc
    67141 gttaagctgg tgtccgaacc aggcgttggc accatcgcga ctggcgtggc gaaagcttat
    67201 gcggacttga tcaccatcgc gggctatgac ggcggcaccg gcgcaagccc gctgtcgtca
    67261 gtgaaatatg caggctgccc gtgggaactc ggtctggtgg aaacccaaca ggcgctggtt
    67321 gccaacggct tgcgtcataa aattcgtttg caggttgatg gcggcctgaa aacgggtgtc
    67381 gatatcatca aagcggcgat tctcggcgca gaaagcttcg gcttcggcac tggcccaatg
    67441 gtggcgctcg gctgtaaata tctgcgtatt tgccacctga acaactgcgc cacgggtgta
    67501 gcaactcagg atgacaaact gcgtaagaat cactatcacg gcctgccgtt caaggtgact
    67561 aattactttg agtttatcgc ccgtgaaacc cgcgagctga tggcacagct gggagtaacg
    67621 cgtctggtgg atctgattgg tcgcaccgac ctgctgaaag agctggacgg tttcaccgcc
    67681 aaacagcaga aactggcgct gtcgaagctg ctggagactg ccgagccaca tgcaggtaag
    67741 tcactctact gcactgaaaa caacccgccg tttgataacg gcctactgaa cgcgcagttg
    67801 ctgcaacagg cgaaaccgtt tgtcgatgaa cgccagagca aaaccttctg gttcgatatc
    67861 cgcaacaccg accgttctgt cggcgcgtcg ctttcaggct atatcgccca gacgcacggc
    67921 gatcaggggc tggcagccga tcctatcaaa gcgtacttca acggcaccgc aggccagagt
    67981 ttcggtgtgt ggaacgcggg cggcgtggaa ctgtacctga ccggggatgc caacgactat
    68041 gtcggtaaag gcatggcggg cggcttaatc gccattcgtc ctccggttgg ttccgccttc
    68101 cgcagccatg aagcaagcat tatcggcaac acctgcctgt atggcgcgac cggtggtcgt
    68161 ctgtatgccg caggccgcgc gggtgaacgt ttcggcgtgc ggaactccgg tgctatcacc
    68221 gtggtagaag gcattggcga caacggctgt gaatatatga cgggcggtat tgtctgtatt
    68281 ctgggtaaaa ccggcgttaa cttcggcgcg ggcatgaccg gcggcttcgc ttacgttctc
    68341 gatgaaagcg gtgatttccg caaacgcgtt aacccggaac tggtcgaagt cttaagcgtc
    68401 gacgatctgg cgatccatga agagcatctg cgcggtctta tcaccgagca tgttcagcat
    68461 accggttctc agcgcggtga agagattctg gcgaactggt caaccttcgc cacgaaattc
    68521 gcgctggtta aaccgaagtc cagtgatgta aaagcactgc tgggtcaccg tagtcgtagc
    68581 gcagcagagt tgcgcgtgca ggcgcagtaa ggggtagcaa caatgagtca gaatgtttat
    68641 caatttatcg acctgcagcg cgttgatccg ccaaagaaac cgctgaagat ccgtaaaatt
    68701 gagtttgttg aaatttacga gccgttttcc gaaggccagg ccaaagcgca ggctgaccgc
    68761 tgtctgtcgt gcggcaaccc atactgcgag tggaaatgcc cggtacacaa ctacatccca
    68821 aactggctga agctcgccaa cgaggggcgt atttttgaag cggcagaact gtcgcatcag
    68881 accaacacac tgccggaagt atgcggacgt gtctgcccgc aagaccgtct gtgcgaaggt
    68941 tcctgcaccc tgaacgatga gttcggtgcg gtgaccatcg gcaacattga gcgttatatc
    69001 aatgataaag cgttcgaaat gggctggcgt ccggatatgt ccggcgtgaa gcaaaccggt
    69061 aaaaaagtgg cgattatcgg tgcaggcccg gcaggtctgg cgtgtgcgga tgtcctgacg
    69121 cgtaacggcg taaaagccgt tgtcttcgac cgtcatccag aaattggcgg gctgctgacc
    69181 ttcggtattc cggccttcaa gctggaaaaa gaggtaatga cgcgtcgccg tgaaatcttc
    69241 accggcatgg gtattgaatt caaactcaat accgaagtgg gccgcgacgt acagctggac
    69301 gatctgctga gtgattacga tgccgtgttc cttggcgttg ggacttatca gtcaatgcgc
    69361 ggcgggctgg aaaacgaaga cgccgatggc gtgtacgcag cgctgccgtt ccttatcgcc
    69421 aacaccaaac agttaatggg ctttggtgaa acccgcgacg aaccgttcgt cagcatggaa
    69481 ggcaaacgcg tggtagtact tggcggtggc gacactgcga tggactgcgt gcgcacgtct
    69541 gtacgccagg gggcaaagca cgttacctgt gcctatcgtc gtgatgaaga gaacatgccg
    69601 ggttcccgcc gcgaagtgaa aaacgcgcgg gaagaaggcg tagagttcaa attcaacgtc
    69661 cagccactgg ggattgaagt gaacggtaac ggcaaagtca gcggcgtaaa aatggtacgt
    69721 accgaaatgg gcgaaccgga tgccaaaggc cgtcgccgcg cggagatcgt tgcaggttcc
    69781 gaacatatcg ttccggcaga tgcggtgatc atggcgtttg gtttccgtcc acacaacatg
    69841 gaatggctgg caaaacacag cgtcgagctg gattcacaag gccgcatcat cgctccggaa
    69901 ggcaacgaca acgctttcca gaccagcaac ccgaaaatct ttgctggcgg cgatatcgtc
    69961 cgtggttccg atctggtcgt taccgccatc gccgaaggtc gcaaagcggc agacggcatt
    70021 atgaactggc tggaagttta agctctgtcc cagcgccgcc tcctcagaaa agagtcggcg
    70081 ctgaaattct cctttctttc tggaaatcgc ctcgcataat ctgaagaaaa ccaatgtaat
    70141 acgctcatct ggattgttac cggtagatgc ccggcgtata gctgattcca ttattcaacg
    70201 cagcgccata taaagggaca gataatggaa tcgctctcag agagaacctc aacaggctac
    70261 cagcaaatcc acaacggcat tattcatctg gtcgatagcg cccgaacgga aacggtacgt
    70321 agcgttaacg cgctgatgac cgcgacgtac tgggaaattg gccgacggat tgtcgaattt
    70381 gaacaaggtg gtgaggccag agcagcttat ggtgcacagt tgattgatcg gctatcgaag
    70441 gatttaagtc gacggtataa acgtggggtc tctgcaaaaa acttacgcca aatgaggctt
    70501 ttttacctct tttttcaaca tgttgaaatt cgccagacag tgtctggcga attaacacca
    70561 ttgggaattc cgcagacacc atctgcagaa ttcccatcgg caaagatttg gcagacgctg
    70621 tctgccaaat tgagaggctg tttgatattt ccacagcttt tgtattgcat ctcctatttt
    70681 cggtttgtat attcatgcaa ataaacgaag
//
LOCUS       DAOFMX010000007        67495 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.007, whole genome shotgun sequence.
ACCESSION   DAOFMX010000007 DAOFMX010000000
VERSION     DAOFMX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.007"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..227
                     /locus_tag="QBA20_000590"
     CDS             <1..227
                     /locus_tag="QBA20_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HDQ6291244.1"
                     /translation="SNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDK
                     YGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            647..1262
                     /locus_tag="QBA20_000591"
                     /pseudo
     CDS             647..1262
                     /locus_tag="QBA20_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1365..1688
                     /locus_tag="QBA20_000592"
     CDS             1365..1688
                     /locus_tag="QBA20_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HDQ6291245.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="QBA20_000593"
     CDS             complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="QBA20_000593"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDQ6291246.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="QBA20_000594"
     CDS             complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="QBA20_000594"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDQ6291247.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="QBA20_000595"
     CDS             complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="QBA20_000595"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDQ6291248.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="QBA20_000596"
     CDS             complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="QBA20_000596"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDQ6291249.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="QBA20_000597"
     CDS             complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="QBA20_000597"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDQ6291250.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="QBA20_000598"
     CDS             complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="QBA20_000598"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDQ6291251.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="QBA20_000599"
     CDS             complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="QBA20_000599"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDQ6291252.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="QBA20_000600"
     CDS             complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="QBA20_000600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDQ6291253.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="QBA20_000601"
     CDS             complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="QBA20_000601"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDQ6291254.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="QBA20_000602"
     CDS             complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="QBA20_000602"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDQ6291255.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="QBA20_000603"
     CDS             complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="QBA20_000603"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDQ6291256.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="QBA20_000604"
     CDS             complement(15658..16320)
                     /gene="nuoB"
                     /locus_tag="QBA20_000604"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDQ6291257.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="QBA20_000605"
     CDS             complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="QBA20_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDQ6291258.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="QBA20_000606"
     CDS             complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="QBA20_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDQ6291259.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19268..20485
                     /gene="alaA"
                     /locus_tag="QBA20_000607"
     CDS             19268..20485
                     /gene="alaA"
                     /locus_tag="QBA20_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDQ6291260.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20569..21168
                     /gene="yfbR"
                     /locus_tag="QBA20_000608"
     CDS             20569..21168
                     /gene="yfbR"
                     /locus_tag="QBA20_000608"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDQ6291261.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21227..23059)
                     /locus_tag="QBA20_000609"
     CDS             complement(21227..23059)
                     /locus_tag="QBA20_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDQ6291262.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23146..23796)
                     /gene="hxpA"
                     /locus_tag="QBA20_000610"
     CDS             complement(23146..23796)
                     /gene="hxpA"
                     /locus_tag="QBA20_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDQ6291263.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23807..24301)
                     /locus_tag="QBA20_000611"
     CDS             complement(23807..24301)
                     /locus_tag="QBA20_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDQ6291264.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24384..24839)
                     /locus_tag="QBA20_000612"
     CDS             complement(24384..24839)
                     /locus_tag="QBA20_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDQ6291265.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25177..26379
                     /gene="ackA"
                     /locus_tag="QBA20_000613"
     CDS             25177..26379
                     /gene="ackA"
                     /locus_tag="QBA20_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDQ6291266.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26454..28598
                     /gene="pta"
                     /locus_tag="QBA20_000614"
     CDS             26454..28598
                     /gene="pta"
                     /locus_tag="QBA20_000614"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDQ6291267.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28788..30308
                     /gene="yfcC"
                     /locus_tag="QBA20_000615"
     CDS             28788..30308
                     /gene="yfcC"
                     /locus_tag="QBA20_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDQ6291268.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30341..30883)
                     /gene="yfcD"
                     /locus_tag="QBA20_000616"
     CDS             complement(30341..30883)
                     /gene="yfcD"
                     /locus_tag="QBA20_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDQ6291269.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30941..31492)
                     /gene="yfcE"
                     /locus_tag="QBA20_000617"
     CDS             complement(30941..31492)
                     /gene="yfcE"
                     /locus_tag="QBA20_000617"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDQ6291270.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31548..32192)
                     /gene="yfcF"
                     /locus_tag="QBA20_000618"
     CDS             complement(31548..32192)
                     /gene="yfcF"
                     /locus_tag="QBA20_000618"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDQ6291271.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32328..32975
                     /gene="yfcG"
                     /locus_tag="QBA20_000619"
     CDS             32328..32975
                     /gene="yfcG"
                     /locus_tag="QBA20_000619"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDQ6291272.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPPDGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33032..33394
                     /gene="folX"
                     /locus_tag="QBA20_000620"
     CDS             33032..33394
                     /gene="folX"
                     /locus_tag="QBA20_000620"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDQ6291273.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33415..34308
                     /locus_tag="QBA20_000621"
     CDS             33415..34308
                     /locus_tag="QBA20_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDQ6291274.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34356..35246)
                     /gene="rpnB"
                     /locus_tag="QBA20_000622"
     CDS             complement(34356..35246)
                     /gene="rpnB"
                     /locus_tag="QBA20_000622"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDQ6291275.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35443..36216)
                     /gene="hisP"
                     /locus_tag="QBA20_000623"
     CDS             complement(35443..36216)
                     /gene="hisP"
                     /locus_tag="QBA20_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDQ6291276.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36224..36940)
                     /locus_tag="QBA20_000624"
     CDS             complement(36224..36940)
                     /locus_tag="QBA20_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6291277.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTVLAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36937..37623)
                     /gene="hisQ"
                     /locus_tag="QBA20_000625"
     CDS             complement(36937..37623)
                     /gene="hisQ"
                     /locus_tag="QBA20_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDQ6291278.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37713..38495)
                     /gene="hisJ"
                     /locus_tag="QBA20_000626"
     CDS             complement(37713..38495)
                     /gene="hisJ"
                     /locus_tag="QBA20_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDQ6291279.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38716..39498)
                     /gene="argT"
                     /locus_tag="QBA20_000627"
                     /pseudo
     CDS             complement(38716..39498)
                     /gene="argT"
                     /locus_tag="QBA20_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
     gene            complement(39764..40333)
                     /gene="ubiX"
                     /locus_tag="QBA20_000628"
     CDS             complement(39764..40333)
                     /gene="ubiX"
                     /locus_tag="QBA20_000628"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDQ6291280.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40428..41945)
                     /gene="purF"
                     /locus_tag="QBA20_000629"
     CDS             complement(40428..41945)
                     /gene="purF"
                     /locus_tag="QBA20_000629"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDQ6291281.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41982..42470)
                     /gene="cvpA"
                     /locus_tag="QBA20_000630"
     CDS             complement(41982..42470)
                     /gene="cvpA"
                     /locus_tag="QBA20_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDQ6291282.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42729..43391)
                     /gene="dedD"
                     /locus_tag="QBA20_000631"
     CDS             complement(42729..43391)
                     /gene="dedD"
                     /locus_tag="QBA20_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDQ6291283.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43381..44649)
                     /gene="folC"
                     /locus_tag="QBA20_000632"
     CDS             complement(43381..44649)
                     /gene="folC"
                     /locus_tag="QBA20_000632"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDQ6291284.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44719..45633)
                     /gene="accD"
                     /locus_tag="QBA20_000633"
     CDS             complement(44719..45633)
                     /gene="accD"
                     /locus_tag="QBA20_000633"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDQ6291285.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45789..46448)
                     /locus_tag="QBA20_000634"
     CDS             complement(45789..46448)
                     /locus_tag="QBA20_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ6291286.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46531..47343)
                     /gene="truA"
                     /locus_tag="QBA20_000635"
     CDS             complement(46531..47343)
                     /gene="truA"
                     /locus_tag="QBA20_000635"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDQ6291287.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47343..48356)
                     /locus_tag="QBA20_000636"
     CDS             complement(47343..48356)
                     /locus_tag="QBA20_000636"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDQ6291288.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48422..49558)
                     /gene="pdxB"
                     /locus_tag="QBA20_000637"
     CDS             complement(48422..49558)
                     /gene="pdxB"
                     /locus_tag="QBA20_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDQ6291289.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49657..50652
                     /gene="flk"
                     /locus_tag="QBA20_000638"
     CDS             49657..50652
                     /gene="flk"
                     /locus_tag="QBA20_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDQ6291290.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50649..51827)
                     /locus_tag="QBA20_000639"
     CDS             complement(50649..51827)
                     /locus_tag="QBA20_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6291291.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52082..53323)
                     /gene="fabB"
                     /locus_tag="QBA20_000640"
     CDS             complement(52082..53323)
                     /gene="fabB"
                     /locus_tag="QBA20_000640"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDQ6291292.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53482..55488
                     /gene="mnmC"
                     /locus_tag="QBA20_000641"
     CDS             53482..55488
                     /gene="mnmC"
                     /locus_tag="QBA20_000641"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDQ6291293.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55609..55887)
                     /locus_tag="QBA20_000642"
     CDS             complement(55609..55887)
                     /locus_tag="QBA20_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDQ6291294.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55921..56469)
                     /gene="epmC"
                     /locus_tag="QBA20_000643"
     CDS             complement(55921..56469)
                     /gene="epmC"
                     /locus_tag="QBA20_000643"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDQ6291295.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56469..57278)
                     /locus_tag="QBA20_000644"
     CDS             complement(56469..57278)
                     /locus_tag="QBA20_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDQ6291296.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57278..58102)
                     /gene="mepA"
                     /locus_tag="QBA20_000645"
     CDS             complement(57278..58102)
                     /gene="mepA"
                     /locus_tag="QBA20_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDQ6291297.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58106..59191)
                     /gene="aroC"
                     /locus_tag="QBA20_000646"
     CDS             complement(58106..59191)
                     /gene="aroC"
                     /locus_tag="QBA20_000646"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDQ6291298.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59226..60158)
                     /gene="prmB"
                     /locus_tag="QBA20_000647"
     CDS             complement(59226..60158)
                     /gene="prmB"
                     /locus_tag="QBA20_000647"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDQ6291299.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60324..60875
                     /gene="smrB"
                     /locus_tag="QBA20_000648"
     CDS             60324..60875
                     /gene="smrB"
                     /locus_tag="QBA20_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDQ6291300.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMYGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60979..61869)
                     /locus_tag="QBA20_000649"
     CDS             complement(60979..61869)
                     /locus_tag="QBA20_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDQ6291301.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61856..62380)
                     /locus_tag="QBA20_000650"
     CDS             complement(61856..62380)
                     /locus_tag="QBA20_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6291302.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62377..62847)
                     /locus_tag="QBA20_000651"
     CDS             complement(62377..62847)
                     /locus_tag="QBA20_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6291303.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62844..63392)
                     /locus_tag="QBA20_000652"
     CDS             complement(62844..63392)
                     /locus_tag="QBA20_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6291304.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63397..64119)
                     /locus_tag="QBA20_000653"
                     /pseudo
     CDS             complement(63397..64119)
                     /locus_tag="QBA20_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64139..64861)
                     /locus_tag="QBA20_000654"
     CDS             complement(64139..64861)
                     /locus_tag="QBA20_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDQ6291305.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64921..66780)
                     /locus_tag="QBA20_000655"
     CDS             complement(64921..66780)
                     /locus_tag="QBA20_000655"
                     /inference="COORDINATES: protein motif:HMM:NF011812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HDQ6291306.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGRTAVRSVISAVSMTRLTIS"
     gene            complement(66862..67425)
                     /locus_tag="QBA20_000656"
     CDS             complement(66862..67425)
                     /locus_tag="QBA20_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6291307.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16859 a        18259 c        16747 g        15630 t
ORIGIN      
        1 aaagcaattc tgacagcgcc ccctatacgt tattaaaaaa ctatgctgac agatttaaaa
       61 aatattcagc agaagagcag tacgaaattg attttcaaca tcgactggta aacagaaatt
      121 gttatttaga taaatatggc gatgcacgaa tcaatgcttc ttatacacaa ttagaaataa
      181 aacactcaca acccaaaaat agagcatccg gcaaaagagt gagttaacat cattgttaat
      241 caattcgacc agtcagtaaa tatgctgact ggtcgaacat aatttgctaa aaagtcatca
      301 ctgactgatg tcagtcccac catctgccag ttcaatctac gggtatttcc agcatatcgc
      361 ggcacgcgat tgctggtctg ttgatgtagt ggattgtagt tgctgacttt tcttaccctc
      421 agatttctgc cccacttgtc aattcaatct gccaataaat aacataatta tatttttatt
      481 cctcacagaa attccttttt aaaaatagcg aacccactgt aaaaaacact ttttgtatac
      541 ctctaccaag cgtaataaaa taaacgctca taattcatta taaataataa tgaaataacg
      601 ccttttattg ataatttaca tatgagaaaa attttccttt tgttatatta tttctgtttt
      661 cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca
      721 cgtaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
      781 taatgccggc aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt
      841 cagtagcggt gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc
      901 ctggaagcca gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact
      961 ttattgctgt ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat
     1021 tgttgtcatc ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta
     1081 ttgatttttc tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg
     1141 acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga
     1201 attatcagaa aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat
     1261 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt
     1321 ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct
     1381 attttgcaga aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt
     1441 tgatggataa tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa
     1501 gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag
     1561 tgaccttttc agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa
     1621 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     1681 ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact
     1741 tacaaactaa cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt
     1801 ggccatacac ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg
     1861 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg
     1921 ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc
     1981 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag
     2041 cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg
     2101 atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga
     2161 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     2221 gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc
     2281 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc
     2341 tggctcaacg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact
     2401 acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     2461 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2521 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2581 atcatcatgc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt
     2641 ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac
     2701 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa
     2761 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata
     2821 ccgaggaaca gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca
     2881 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2941 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3001 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     3061 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3121 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3181 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3241 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3301 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     3361 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     3421 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3481 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3541 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3601 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     3661 taaagctgac cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     3721 attaccgcgc cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc
     3781 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc
     3841 cacgcaccgt agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc
     3901 gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc
     3961 aagatccccg cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4021 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc
     4081 aacagatatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4141 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc
     4201 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4261 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4321 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4381 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4441 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca
     4501 atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4561 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4621 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     4681 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     4741 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     4801 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc
     4861 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4921 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4981 cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg
     5041 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5101 cttgacgaac actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact
     5161 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca
     5221 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     5281 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5341 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5401 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5461 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5521 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5581 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5641 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     5701 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     5761 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5821 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5881 cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag
     5941 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag
     6001 cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg
     6061 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6121 gccgtcagca aagtgcgctg gtgccagttc caccatttcg cggaagttca gggtgcccag
     6181 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6241 gaacgctttc attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6301 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag
     6361 aaccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6421 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6481 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6541 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6601 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     6661 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     6721 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6781 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6841 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     6901 cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     6961 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     7021 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7081 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7141 ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7201 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7261 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc
     7321 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7381 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7441 ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg
     7501 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7561 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7621 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7681 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     7741 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7801 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     7861 gcagcgtgag aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     7921 tttttgcagc gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca
     7981 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8041 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8101 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8161 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8221 agcgtcagcg gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc
     8281 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8341 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8401 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8461 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8521 tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8581 atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8641 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8701 gaagcacgca tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8761 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8821 gcaaccaccc aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag
     8881 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8941 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9001 ttaggtccat aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9061 gccccacagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9121 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9181 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     9241 tcagtccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga
     9301 cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9361 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9421 ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9481 tggtgaagta atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9541 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9601 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca
     9661 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9721 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9781 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc cgccagttcc gggattttcg
     9841 ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg
     9901 tgctgtgcag cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat
     9961 aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac
    10021 cgtcgctttc agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg
    10081 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10141 tagcagaagc gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg
    10201 tttccagttc ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10261 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10321 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10381 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcaact
    10441 caggttcgat accgtcaacc gctggtgcgg agttatccag cgcatgggcg atggcaaaac
    10501 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10561 gagtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10621 ttgccgcaat ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt
    10681 tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10741 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10801 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10861 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10921 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10981 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11041 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11101 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11161 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11221 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11281 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11341 tgtcgtgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11401 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11461 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg
    11521 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11581 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11641 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11701 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    11761 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11821 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11881 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    11941 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12001 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12061 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12121 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12181 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12241 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12301 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12361 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12421 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12481 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12541 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12601 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    12661 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    12721 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    12781 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    12841 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12901 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12961 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13021 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc
    13081 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13141 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13201 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13261 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13321 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13381 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13441 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13501 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13561 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13621 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13681 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13741 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13801 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13861 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    13921 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    13981 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14041 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14101 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14161 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14221 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14281 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14341 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14401 cggcatccag tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc
    14461 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14521 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14581 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    14641 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    14701 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    14761 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    14821 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    14881 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcgt tttcggtgcc
    14941 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15001 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgc gccgggtaat ctttacgcag
    15061 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15121 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15181 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15241 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15301 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15361 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15421 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15481 ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15541 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15601 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15661 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15721 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15781 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15841 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15901 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15961 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16021 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16081 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16141 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16201 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16261 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16321 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16381 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16441 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16501 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16561 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16621 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16681 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    16741 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16801 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16861 aaaaacctta caataactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    16921 cctttctata accttttgtc aactttaaca aaagcttctt cacattagtt tacataatat
    16981 caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta
    17041 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17101 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17161 gttattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag
    17221 gtgatttttt tcaggcgatt atttgtgcat tcgggacgtg aatctctggt gattgaaaaa
    17281 taaacagttt tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct
    17341 ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga attatctatc
    17401 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17461 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17521 cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg
    17581 taagccatct actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc
    17641 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17701 agccagtcgc catggaatat ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17761 acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc
    17821 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17881 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17941 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    18001 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag
    18061 attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta
    18121 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    18181 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    18241 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    18301 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    18361 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18421 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18481 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18541 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18601 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18661 gcgaacacgt acaaacagca atggtgaagc ggcatcaaga gcagggatcc gtcatttatg
    18721 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18781 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18841 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18901 ttcacacctt acccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa
    18961 tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa
    19021 ttatcttaca gataaaaaaa ccagacttgc aattaagaat tagaacaatt cactatataa
    19081 cattgcatgt aaagcatata cacctcatta ttttgtcatt attaagttat taacagcaca
    19141 atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg
    19201 gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc aaaaaaggtc
    19261 aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt atgacatccg
    19321 tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg tactgaaact
    19381 gaacatcggc aacccagccc cattcggttt tgacgcgcca gatgaaatcc tcgttgacgt
    19441 gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaagggcttt actccgcgcg
    19501 taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg tggaagatat
    19561 ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat tgctgaacag
    19621 cggggacgaa atgttggttc ctgcaccaga ttacccactc tggaccgcgg cggtttcgct
    19681 ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt tcccggacct
    19741 cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg attgttatta tcaacccaaa
    19801 taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg agattgcacg
    19861 tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct acgacgatgc
    19921 tgagcatcat tcaattgcgc cgctggcacc tgacctgctg accattacct ttaacggact
    19981 gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga acgggccgaa
    20041 aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc
    20101 taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga gcatcagtga
    20161 atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg aactgatcaa
    20221 cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt tcccgaaaat
    20281 cgacgccaaa cgctttaaca ttcatgacga tcagaaaatg gtgctggatt tcctgttgca
    20341 ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg atcacttccg
    20401 cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt
    20461 cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc atccggcagt
    20521 gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat gaaacagagc
    20581 catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct aatgcgcaac
    20641 gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg
    20701 gcagctatca aaaatcggaa atttggcggt aatgtcaacg ccgaacgtat cgctttactg
    20761 gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc ggtgaaatac
    20821 ttcaatgcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca gcagaaactg
    20881 gtcgatatgg ttccggaaga actgcgggat atctttgcgc cgttaattga cgagcatgca
    20941 tatagcgatg aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc atatctgaaa
    21001 tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac gcgactggaa
    21061 gcgacgcttg aagcgcgtcg cagccaggag atggactact tcatggaagt atttgttccc
    21121 agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc agccggagtc
    21181 tgcgtcgcat cagtcaataa gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca
    21241 gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt acccccaact
    21301 tcacaaagtc actgaagctg taatttcccg gacctaaaac cagggtgtta acaggtgaag
    21361 acaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgca aatggataag
    21421 gcgacactcc catcgttttg gcagcagcca gcgcaatcgg agccatcaac accgccgtcg
    21481 cggtattaga gataaacagc ccaataaccg ccgacaagac aaacaaacac cccagcatca
    21541 tatgtggccc ataaccgccg ccaatatcca tcagcccttt caccgccagc gcgacacctc
    21601 ccgttttctg taatgccaca gcaaacggca tcatcccaac gatcaaaata atgctcggcc
    21661 agtgaatgga tttataggcg ctttcagcat ctatacagcg gaatttcccc atcagcaggc
    21721 aggcgatgat agcggcaaca ggattaggaa tttcatttgt cagcattaac gccaccatta
    21781 gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct tcactcacct
    21841 cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata ccgatcagtt
    21901 tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc gccagcgaac
    21961 cttccagcgc cacgccattg cgcttgagcc ccaccacatt cagtccgtag cgggtacgaa
    22021 aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat gaaatctctg
    22081 ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc atcggctcca
    22141 gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata tcaataagca
    22201 aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg cgacgaaaac
    22261 gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat ttgagatcat
    22321 ccagccgttg acctatcatt ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg
    22381 tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg
    22441 gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa atacccagca
    22501 ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga tagccttcac
    22561 gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc atcatgccgc
    22621 taatcagccc ggcaaaactt aacggcatca tcagacgtga cggcgaagtt tgcatacgca
    22681 tggcaacgct taacaccacg ggaataaaga tagcgacaac gctggttgaa ctcataaacg
    22741 cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca ctgttgcccg
    22801 ccactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc aaaccatcgc
    22861 caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg gaaaatactt
    22921 cggggaccgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat
    22981 ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc
    23041 aaatcaattc accgttcaca acttatcctt gttaattgag ggggatgact tgattctgcc
    23101 ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact gaataataac
    23161 atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga ccaaatcgac
    23221 ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatggc aacccgccgc
    23281 caggccagaa agcacgccag cgggagcatc ttccaccacc acacactcct gcggcgcaag
    23341 ccccagcagc tgcgcaccta acagatacgc atcaggttct ggttttccgc gcttcactcg
    23401 ctcagcggtt acaaacacct ctggtgcggg aagccccgct attttatggc gcgctcgcgc
    23461 taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat tcaaatgatt
    23521 gagtaaggcg attgccccag gaagcgcggt aataccttcg gtttccgtgg cctcgatttg
    23581 ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca taaaatggcg
    23641 caaagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg gcgctaaccc
    23701 atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat ccaccagcgt
    23761 tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag gcgttgataa
    23821 tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc cacacattaa
    23881 gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccatga gtcccagcgt
    23941 caaaatgggt atagcgccct tccacattaa ccataaagcg gacataaccg aggtaacgtg
    24001 cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg gattgctggt
    24061 cctgcaaatt agaccaggaa acatacaacg catgatacat ctccataatg tcgatgatag
    24121 tgcggcaggt ttcttctttc agctcgccaa actcgcgatc caattcacgc atctgtaatc
    24181 cgtaaccacg ctcaataatt gtttgcaggc gacggtaacg ttcagcattt gccggatcga
    24241 gcatagtcat catcttgtac tgattagaca aaataagacg ttgcgcgttg gtcatttcca
    24301 ttgttgactc ctgtatcact ctactacggt gaaaaaaaag aaggctgagt atgccttctt
    24361 ttatatgcgt aatcaggggt caattacaaa tcatcaagga aagttttatc cagttgtttg
    24421 aaggcgcgct taagcgtgtc agctaatgcc tggtaatcag gcttgccttc aacgggtgcc
    24481 aacacctgtc cagactcctg caatttaccg cgaacttcat aaaaccagtt aaggattgca
    24541 gggggtaatg gcgttacaga acgcttgccc agccaccaca atccctgcat gggtaaactt
    24601 aaggcgaaca gtgcagtggc aactgccggc ccaagcagac cgcccagggc aatctgccag
    24661 cagagagtaa atacggcgat cggcggcata aaacggatcg cataacgcgt catcttgata
    24721 acgcgatttt cgacaaagac cggggcaagg cgtttttcca gtggccacgt ctttgagtaa
    24781 tgctgtccgc ggcgaaacaa gctaaaaaaa ttaacagaac gattatccgg cgttgacatg
    24841 cttcacctca acttcacata taaagattca aaaatttgtg caaattcaca actcagcggg
    24901 acaacgttca aaacattttg tcttccatac ccactatcag gtatccttta gcagcctgaa
    24961 ggcctaagta gtacatattc attgagtcgt caaattcata tacattatgc cattggctga
    25021 aaattacgca aaatggcata gactcaagat atttcttcca tcatgcaaaa aaaatttgca
    25081 gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc tacgctctat ggctccctga
    25141 cgttttttta gccacgtatc aattataggt acttccatgt cgagtaagtt agtactggtt
    25201 ctgaactgcg gtagttcttc actgaaattt gccatcatcg atgcagtaaa tggtgaagag
    25261 tacctttctg gtttagccga atgtttccac ctgcccgaag cacgtatcaa atggaaaatg
    25321 gacggcaata aacaggaagc ggctttaggt gcaggcgccg ctcacagcga agcgctcaac
    25381 tttatcgtta atactattct ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc
    25441 ggtcaccgta tcgtacacgg cggcgaaaag tataccagct ccgtagtgat cgatgagtct
    25501 gttattcagg gtatcaaaga tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg
    25561 atcggtatcg aagaagctct gaaatctttc ccacagctga aagacaaaaa cgttgctgta
    25621 tttgacaccg cgttccacca gactatgccg gaagagtctt acctctacgc cctgccgtac
    25681 aacctgtaca aagagcacgg catccgtcgt tacggcgcgc acggcaccag ccacttctat
    25741 gtaacccagg aagcggcaaa aatgctgaac aaaccggtag aagaactgaa catcatcacc
    25801 tgccacctgg gcaacggtgg ttccgtttct gctatccgca acggtaaatg cgttgacacc
    25861 tctatgggtc tgaccccgct ggaaggtctg gtcatgggta cccgttctgg tgatatcgat
    25921 ccggcgatca tcttccacct gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa
    25981 ctgctgacca aagagtctgg cctgctgggt ctgaccgaag tgaccagcga ctgccgctat
    26041 gttgaagaca actacgcgac gaaagaagac gcgaagcgcg caatggacgt ttactgccac
    26101 cgcctggcga aatacatcgg tgcctacact gcgctgatgg atggtcgtct ggacgctgtt
    26161 gcattcactg gtggtatcgg tgaaaatgcc gcgatggttc gtgaactgtc tctgggcaaa
    26221 ctgggcgtgc tgggctttga agttgatcat gaacgcaacc tggctgcacg tttcggcaaa
    26281 tctggtttca tcaacaaaga aggtacccgt cctgcggtgg ttatcccaac caacgaagaa
    26341 ctggttatcg cgcaagacgc gagccgcctg actgcctgat ttcacaccgc cagctcagct
    26401 ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga aagaggataa accgtgtccc
    26461 gtattattat gctgatccct accggaacca gcgtcggtct gaccagcgtc agccttggcg
    26521 tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc
    26581 cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat cgtgcgtgcg aactcttcca
    26641 ccacgacggc cgctgaaccg ctgaaaatga gctacgttga aggtctgctt tccagcaatc
    26701 agaaagatgt gctgatggaa gagatcgtcg caaactacca cgctaacacc aaagacgctg
    26761 aagtcgttct ggttgaaggt ctggtcccga cacgtaagca ccagtttgcc cagtctctga
    26821 actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg
    26881 acaccccgga acagctgaaa gagcgtatcg aactgacccg caacagcttc ggtggtgcca
    26941 aaaacaccaa catcaccggc gttatcgtta acaaactgaa cgcaccggtt gatgaacagg
    27001 gtcgtactcg cccggatctg tccgagattt tcgacgactc ttccaaagct aaagtaaaca
    27061 atgttgatcc ggcgaagctg caagaatcca gcccgctgcc ggttctcggc gctgtgccgt
    27121 ggagctttga cctgatcgcg actcgtgcga tcgatatggc tcgccacctg aatgcgacca
    27181 tcatcaacga aggcgacatc aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca
    27241 gcattccgca catgctggag cacttccgtg ccggttctct gctggtgact tccgcagacc
    27301 gtcctgacgt gctggtggcc gcttgcctgg cagccatgaa cggcgtagaa atcggtgccc
    27361 tgctgctgac tggcggctac gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt
    27421 tcgctaccgg cctgccggta tttatggtga acaccaacac ctggcagacc tctctgagcc
    27481 tgcagagctt caacctggaa gttccggttg acgatcacga acgtatcgag aaagttcagg
    27541 aatacgttgc taactacatc aacgctgact ggatcgaatc tctgactgcc acttctgagc
    27601 gcagccgtcg tctgtctccg ccagcgttcc gttatcagct gactgaactg gcgcgcaaag
    27661 cgggcaaacg tatcgttctg ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta
    27721 tctgtgctga acgtggtatc gcaacttgcg tactgctggg taatccggca gagatcaacc
    27781 gtgttgcagc gtctcagggt gtagaactag gtgcaggcat tgaaatcgtt gatccagaag
    27841 tggttcgcga aagctatgtt ggtcgtctgg tcgagctgcg taagaacaaa ggcatgaccg
    27901 aaaccgttgc ccgcgaacag ctggaagaca acgtggtgct cggtacgctg atgctggaac
    27961 aggatgaagt tgatggtctg gtttccggtg ctgttcacac taccgcaaac accatccgtc
    28021 cgccgctgca gctgatcaaa actgcaccgg gcagctccct ggtatcttcc gtgttcttca
    28081 tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc gatcaacccg gatccgaccg
    28141 ctgaacagct ggcagaaatc gcgattcagt ccgctgattc cgctgcggcc ttcggtatcg
    28201 aaccgcgcgt tgctatgctc tcctactcca ccggtacttc tggtgcaggt agcgacgtag
    28261 aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg
    28321 gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact
    28381 ctccggttgc aggtcgcgct accgtgttca tcttcccgga tctgaacacc ggtaacacca
    28441 cctacaaagc ggtacagcgt tctgccgacc tgatctccat cgggccgatg ctgcagggta
    28501 tgcgcaaacc ggttaacgac ctgtcccgtg gcgcactggt tgacgatatc gtctacacca
    28561 tcgcgctgac tgcgattcag tctgcacagc agcagtaatc tcgtcatcat ccacagcttt
    28621 gcgctgcgga tatctgaacc ggaaataatc actatttccg gttttttatt ctcttaattt
    28681 gcattaatcc tttctgatta tcttgcttaa ctgcgctgca tcaatgaatt gcgccatctc
    28741 actttgcata cttaccactt tgttttgtgc aagggaatat ttgcgctatg tccgcaatca
    28801 ctgaatccaa accaacaaga agatgggcaa tgcccgatac gttggtgatt atcttttttg
    28861 ttgctatttt aaccagtctt gccacctggg tagttccggt ggggatgttt gacagtcagg
    28921 aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat
    28981 ttcgcattct gactaacgaa gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga
    29041 cgggcgatga acgcccgggc ctgatgaact tcccgtttga agggttaacc tcaggatcga
    29101 aatacgggac agccgttggc atcatcatgt ttatgctggt gattggcggc gcgtttggca
    29161 ttgtgatgcg tacaggaacc attgataacg gtatcctggc gcttattcgc catacccgcg
    29221 ggaatgaaat tctctttatt cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat
    29281 ttggtatggg agaagaggcc gtcgcctttg ccattatcat cgcaccgcta atggtccggc
    29341 tgggctatga cagtattacc accgtcctgg tgacttatat tgccacgcaa atcggttttg
    29401 ccagttcgtg gatgaacccg ttttgtgtgg ttgttgctca ggggattgcc ggcgttccgg
    29461 tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat cgccactctg attggcctga
    29521 tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg
    29581 agtccgaccg cttctttcgt gaaaagcagg cggatgttga acaacgtccg tttacctttg
    29641 gtgactggct ggtattgatt gtcctgacag ccgtaatggt ctgggtgatt tggggcgtga
    29701 tcgttaatgc ctggtttatt ccagaaattg ccagccagtt cttcaccatg ggtctggtga
    29761 ttggcatcat cggcgttgtt ttccgcctta acggcatgac ggttaatacc atggcttcat
    29821 cctttaccga aggggcgcga atgatgatcg cccctgccct gctggtgggt ttcgccaaag
    29881 ggattttgct gctggttggt aatggtgaag caggtaatgc cagcgtgtta aatactatcc
    29941 tcaacagcat tgccaatgcc attagcggtc tggacaacgc ggtcgcggcc tggtttatgt
    30001 tgctcttcca ggctgtattt aatttcttcg tgacgtccgg ttctggtcag gcggcattaa
    30061 ctatgccgtt actggcaccg cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc
    30121 tggcgttcca gtttggtgat ggcttcagcc acatcattta cccaacctca gcttcgttaa
    30181 tggcaacgct cggtgtgtgc cgggtggact tccgtaactg gctgaaggtg ggcgcgaccc
    30241 tgcttggact gctgtttatt atgtccagcg tcgtagtgat cggcgctcag ttgatgggct
    30301 accactaaaa atgttaagag ccgcaattgc ggctcttttt tcattctgcc gtttcagtct
    30361 ctacggcttc atttttggca ttgcgcttca tccacaacgc cagcgctttc agcgagtctg
    30421 gagtgaactc atcgcagcgt gcggtgattt cttccggcgt cagccagcaa actccactga
    30481 cttcatcttc ctgtagggcg aagggaccgt gagagacgca actgaacaac gcgccccaga
    30541 cacggcaatt tttatcttcg aaatagaact gcccgtgctc ggcaaagggg acaccggcaa
    30601 tgcccaactc ttcttccgct tcgcgacgcg cggattccag cagttgctca tcggcctgaa
    30661 ctacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac
    30721 gctggaccag aattttgccc atgccatcat gcacgacgat gtaagttgca cgatgacgca
    30781 gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat gacttcgttc tcttcattga
    30841 caatatccac ccattcagta cttgccaaac gacgctgttc cattatcagg aaaccttctt
    30901 tttctggcgc tcttacggcg cattttgagt tgtgggtaaa ttacggatta atcgcgacct
    30961 gcgcaatgat actttgatca ttgagtgcga ttacactaag tacgtcatta tccagcatgc
    31021 cataacccgc cggattaccg cctttcggaa tactcaccga gccggggttg aagtggaaaa
    31081 tctccccccg ctgttctgcc actggcagat gggtatgacc gtacaccagc acatcgttct
    31141 ggtttaaagc aggtagattt tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt
    31201 gtttttccag taatacctgt tgccacggcg cggttatcgg gaaatgcagc atcatttgat
    31261 ccacttcgct gtcgcagttg ccacgcacag cgataacctt atgtgccact tcattaagcc
    31321 gttcagcgac tttggctggc gcgtaaccct ccggtaaagc attacgcggg ccgtgattca
    31381 acacgtcgcc aaggatcacc agccactggg caccgctttg gacaaacaac tccacaacac
    31441 gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc atcactcact
    31501 cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc gttgctatca gccagattgc
    31561 ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct gccactggaa tgtcgcataa
    31621 tccaccaggc gttctggcac ctcatcgcca tgtagtacca ggcggttaat cattagcgcc
    31681 agatcagtat cagcaatgca ccattcacca aataaattcg gctgacccag tgctaacaaa
    31741 tgttctgcca tcgcgaacag tttctctgca ctggcttttc cctcggccgt tagtggcgct
    31801 tttttcgccc ccgcaaagac aacatccgtc ggacgctctt cgcggatggg catcagatcg
    31861 ctgcgcagcc aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa
    31921 atacgttccc aggtcggtgg cgcaaatcga tcttccagat actccgcaat ggcagaagat
    31981 tcactcaact caaaatcatc gatttgtaat aacggcacac ggcgtgtctg accgtaacct
    32041 tgccacgtcg gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac
    32101 aggccttttt cctgcaacgc cacccaggcg gataacacat aaggggagaa aaagtgggca
    32161 tctgaccaaa gcgtgatagc gggtttactc attatgtcct cggcaataaa gggggctggc
    32221 tatcaaacta tagagccttt ttgtcactgt cacgtgacga atactcacgt aagtcgccgc
    32281 gctttctata cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg atcgatctct
    32341 atttcgcccc gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg
    32401 attatcgctt gattaaggta gacctgggga aaggcggtca gtttcgtccg gaatttttgc
    32461 gcatttcgcc caacaacaaa attccggcaa ttgttgatca ttctcctccc gatggcggcg
    32521 aaccgttaag cctctttgag tctggtgcca ttttgttgta tctggctgag aaaacaggac
    32581 tctttttgag tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta ttctggcagg
    32641 taggcggact ggggccgatg cttgggcaaa atcatcattt taatcacgca gccccccaaa
    32701 ccattcctta cgctattgaa cgttatcagg ttgaaactca gcgtctttac catgtactaa
    32761 acaagcggct ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt gcggatattg
    32821 cctgctggcc gtgggttaat gcctggactc gcctgcgaat tgacctcgca atgtatccgg
    32881 cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc caccgggcag gcactgctaa
    32941 aagcacaact cggtgatgag cgttcggata gttaacagaa acaggttctc gtgtattatt
    33001 tcatcctaag taaaacaacg gagaacctgc aatggcacaa cctgccgcta ttattcgtat
    33061 aaagaacctt cgtttgcgta cgtttatcgg aattaaggaa gaagaaatta acaaccgtca
    33121 ggatattgtt atcaatgtga cgatccacta ccccgccgat aaagcgcgca ctagcgaaga
    33181 tatcaacgat gcgctgaatt atcgcaccgt aacgaaaaac attattcagc atgtagagaa
    33241 taaccgtttc tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca
    33301 tcactgggtg acgtatgctg aagtggagat cgataaactg cacgcgctgc gctacgccga
    33361 ttcggtatcc atgaccttaa gctggcagcg ttaatcgcca tatcgggagg ctgcatgaac
    33421 atagtgatca ccggagggac gggattaatt ggtcgccatt tgattccgcg tttgctggag
    33481 ctgggccatc aaattacggt agtgacgcgt aacccgcaga aagccagttc cgttctcggc
    33541 cctcgggtga cactatggca agggcttgcc gatcaaagca acctcaacgg cgttgatgcg
    33601 gtaatcaacc tggccggaga accgattgct gataaacgct ggactcacaa gcaaaaagag
    33661 cgtctctgcc aaagccgctg gaatatcacg caaaaactgg tcgatttgat taatgctagc
    33721 gacacgccgc cgtcggtact catttccggc tcggcaacgg gctattatgg cgacttaggt
    33781 gaagtggtgg ttaccgaaga ggaaccgccg cataacgaat ttactcataa actctgcgcc
    33841 cgctgggaag agattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga
    33901 accggcgtgg tactggcacc ggacggcggt attctcggta aaatgctgcc accgtttcgt
    33961 cttggcctgg gcgggccgat tggttccggt cggcagtatc tggcctggat tcatattgat
    34021 gatatggtca acggcattct ctggctgctg gataacgagc tgcgcgggcc atttaatatg
    34081 gtttcgccct acccggtacg caatgaacaa tttgcccatg cgctcggtca tgcgctgcat
    34141 cgcccggcta ttttgcgcgt ccccacaacc gccattcggc tgttaatggg cgaatcttca
    34201 gtactggtat taggtggaca acgcgcgctg cctaaacggc tggaagaagc gggttttgcg
    34261 tttcgctggt acgatttaga agaggcgctg gcggatgtcg ttcgctgatg tggtttacag
    34321 caaacatccg ccagttaact ccctgtgtta caggattagt ggctttgcgc gataagatcg
    34381 tctggtgaaa gtcgggtcac catcataact aactctctgt ctaaacctct atccagcatc
    34441 tcctgagcaa tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat
    34501 ctgtcagcaa tggtcatcag tttctccttt tcttgtggtg cgcgttctgt tatctcacca
    34561 ataaatgcac ggaaacgctg ggcatcccct gtttgtaata cgtaattaaa cagggctttt
    34621 agctgtctgt cattagtgtt ccctgtaact agcagcgaaa caatttggtc gactaatccc
    34681 aacagatcgc gctgacgaat atgtttctga attaactcca acagcgccat tttgcggtgt
    34741 tgcataatct cgtcatccgg caccacggta atatccacca acggaaacgc tgatgaatat
    34801 attttgcggg ctatagcagg ctcggcaaat tcatcaagcc agcagagtga ataaggataa
    34861 ggacttctgc aaccatgata aaacagcatc gggatcacca atggaagctc tttatagccc
    34921 gcatcaagat ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat
    34981 tcttccggct tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt
    35041 ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt
    35101 ggttctagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata
    35161 ttaataaaat tccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc
    35221 ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc
    35281 gacaataacc acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt
    35341 cagaagtatt tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc
    35401 cccttgaaaa tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt
    35461 aaggaactgt tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc
    35521 ttcttctatt ttcccctgat gtaggaaaat gacgtgagta gaaacatggc gagcaaagcc
    35581 catttcgtga gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg
    35641 taacacttcg cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac
    35701 ttctggttcc atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag
    35761 atgcaccgga tatttcccct gcgcacgttc gtctatcccg actttcgcca gatacttcac
    35821 cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac
    35881 gttttccaac accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg
    35941 tgtgcgcagt aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg
    36001 caccagattg atcgtctggc cattgaccac gatcgatcct tcactcggtt tttcgaggaa
    36061 gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac
    36121 atctccggca ttcgcttgca gtgagacccc tttcagcact tcgtgttcgc catagcgttt
    36181 gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag
    36241 gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag
    36301 agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg
    36361 cgttaatatc gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcacag
    36421 tagagtgcag catcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag
    36481 aaggcaaaat aatgcagcga tacattttaa aggtcgagaa gccataggct ctggcggctt
    36541 caatttcccc atgcggaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc
    36601 aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa
    36661 ggaattcggt tcccttaaca atctcaagcg tgtacatgcc ggaatagaac accagcaact
    36721 gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt
    36781 atttattact ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta
    36841 ccgacaaaat aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc
    36901 acagcagcgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc
    36961 cacggagtag cggcgctcaa ggaacagcag cacaccattg gaaacggtgg tgaaaaccag
    37021 gtaaatcacg ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag
    37081 ctgcgtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag
    37141 gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat
    37201 gatccgccga aacacttgcc cgcgagtaaa accgaacgcc gtcgccgcct ctatatgccc
    37261 tttcggcact gccataaacg cgccacggaa cgtttcggta aaataagcac cgtagataaa
    37321 accgagagtg ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc
    37381 ctccgtcacc atgtttagcg caatctgcaa accatagaaa atcagcagca tcaacaccaa
    37441 gtccggcacg cctcgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt
    37501 ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct
    37561 gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatacaa
    37621 catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc
    37681 gaagggagcg ggctttcttt caccgatggc gattaaccac cataaacatc aaaatcgaag
    37741 tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc
    37801 agtgcctcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac
    37861 agtttttcat ctttaacaga cggaccaccg aatttgtaat ctttaccgac gggttgtttg
    37921 aggaaacctt cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc
    37981 aggtcagaat aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc
    38041 cagtgttcat taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct
    38101 ttcagcgact cgactgtcgg ctgaatgtca gaatttttcg ccaccaccag acgagaatcg
    38161 gcagcgtaca gtttgtcggt gaaggcaatt tcttgctgac gtttttccgt aatggaaagc
    38221 gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcggg
    38281 ttttcgacaa acgtacattg cgtattgatg cgcttgcaca actccttcgc cagctcgata
    38341 tcgaaaccaa ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg
    38401 gtaccgatgc ggatgttttg cggaattgca gcaagtgccg cagttgcgct ggagaaggcc
    38461 agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt
    38521 atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa
    38581 aatatcccgc cccgttagac gtattatttt tcattaaaaa tgcttaagta tgcatttcaa
    38641 tgcaccacga tagtgcaaca aacctggcgt gcaccatgat gatcattcca tcaggtacag
    38701 cttcccagcg acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt
    38761 gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc
    38821 agcatcatct ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac
    38881 tgatgagcca gcaaaagcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc
    38941 agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg aatagaccaa
    39001 atcctggttg gcataggcca ccacatccac gcctttacta cgccaggtct cgttagcgta
    39061 agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt
    39121 tggctgaatc ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc
    39181 ggagaaggca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatagcgtc
    39241 gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cctaggtaca
    39301 tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc
    39361 acctttagca tctttcgacg agaacagcgc ataggtggta tcggttccga tacgtaccgt
    39421 ctccggtagc gccgcatagc tggaagccgc tgcggagaga ccgactaata aagacagagc
    39481 gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt
    39541 gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa
    39601 cattcaacgt taaatatgtt aataagacgt tgcattattg tcctgaagtt gaagatagca
    39661 ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt
    39721 gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc
    39781 gggcaaagag atcttcagga agggttattg caaactggtc aagaacacga ttaaccgtct
    39841 gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaacg ggaggcataa
    39901 tcaccgcacc gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg
    39961 tttcacgcac gcagagcacc aacgggcgac gctctttcag caccacatct gccgcacggg
    40021 tcagtaagcc atcagtatag ctatggacaa tgccggacag ggttttgatt gaacagggta
    40081 aaatcaccat ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg
    40141 catcgagcgt gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg
    40201 ataaggtctg gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc
    40261 gcagaacctg taataagcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa
    40321 tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg
    40381 cgggagttgc aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta
    40441 tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt
    40501 aacgtatcga ggaaatcgag gtagccctga tcaacatctt tggtgacgta gacgccgttg
    40561 aacaccgagc attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga
    40621 tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatcaact
    40681 tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg
    40741 aagcgaattt ccggtgccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc
    40801 atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg
    40861 tttttatcgc ggaactcggc gcggttggca ttcagtttac ggcgcacgga cttacgacgc
    40921 agctgctggc ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg
    40981 cggtacggtt tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttctggg
    41041 atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcgcccagt
    41101 ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg
    41161 cgggcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga
    41221 cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc
    41281 aggaaatcaa agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta
    41341 cggttctcgt caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg
    41401 aaagcaacca taccgtggcc gataatcatc gccacacagg catacgcgcc gcggattaag
    41461 cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag
    41521 ttgtccagct cgctggcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg
    41581 tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg
    41641 ttgtgggcaa gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg
    41701 ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc
    41761 tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag
    41821 cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc
    41881 gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg
    41941 cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct aggcaagaaa
    42001 cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa ttgcgggatc
    42061 agctgtgatt tgctccagtc ttcacttttc gacaccccgg taaaggagtc gagaaagaag
    42121 agaatggcgg caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga
    42181 tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca
    42241 ccaacgatca gggtagcgat aaacagtacc gcgatggcaa tcccatttcg aaccagttcg
    42301 tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa
    42361 gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgtggat caggctaacc
    42421 agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca
    42481 cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc
    42541 gtagggattt ccttcccgcg catcaataaa aatggcgctg aaaaaatatt caacgccatc
    42601 gactttttat gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat
    42661 ccgacctacg gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca
    42721 agactgtatt aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc
    42781 aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg
    42841 gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc
    42901 agcttaccga caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca
    42961 taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc
    43021 gcaggtggtg cttcaacctt tggtttaggc ggctccaccg gtttcggctt cggtggggcg
    43081 accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc
    43141 gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc
    43201 ggcaacgcct gggtggcggc tggcatcata tcaggctcat cacgatcgcc cgctttcggc
    43261 accagcggga tagccgcgaa ctcatcctga taatgttttt tctgcccgtc cagcagccct
    43321 ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac
    43381 ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa
    43441 agaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc
    43501 ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt
    43561 ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca
    43621 ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc
    43681 gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc
    43741 gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc
    43801 gctggcaatc ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc
    43861 cgccagcgcc gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt
    43921 gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc
    43981 ccgacgttgt aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc
    44041 cggctcaccg acaattgccg gtttttcgct gcggaagatg cctgctttct cgcgaccaat
    44101 actttcgcga tctggaccca gccagtcggt atgatccagc gcaatactgg ttactaccgc
    44161 gacatcggca tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat
    44221 caccacgtca agttgtgcct gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa
    44281 ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc
    44341 cgattccggc aattcctggc cctgcacacg tacgcgctcg gtataacgca ccagatgagg
    44401 cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca
    44461 ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcaggac
    44521 gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg
    44581 caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt
    44641 gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg
    44701 ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac
    44761 gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct
    44821 cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat
    44881 caggaattca ctgcgctgga atctaggcgg cagtttttcg cgaacggtct gttcgataac
    44941 acgcggaccg gcaaagccga ttaacgcttt aggttcagcg atgttgagat cgcccagcat
    45001 ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa
    45061 gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat
    45121 cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc
    45181 ttcctgcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc
    45241 gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt
    45301 catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagatgcca gacggtcttt
    45361 atacttcttg gagtcacgaa acttcagctc atctttcggc tcaagctcgc tacccagctc
    45421 cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg
    45481 atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg
    45541 accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt
    45601 aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt
    45661 cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa
    45721 tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga
    45781 gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg
    45841 cccggcaaaa tagaaaccac aataatcccg acgatcagca gcttaaggtt atcctgaacc
    45901 atcgggattg taccgaagaa atagcctgcg taggtaaaaa gcagtaccca caacagtgcg
    45961 ccgatcacgt tataagcagc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac
    46021 ggggcgaacg ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt
    46081 ttctcataaa actgatgggt tttgtcgaga taactgcgac ggaaaatttt cgaattggga
    46141 ttactgaata acttttcacc gaacaaccgc ccgatagtgt aattgaccgc gtcacccaca
    46201 atcgcggcga tcagcatcag cactaccatc atatggacgt tgagatcgtt ggtttccagt
    46261 gatgccaacg ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg cattaccacc
    46321 agaccggttt cacagaacaa aattaagaac aaaatggcat aaacccagac gccgtactcc
    46381 gcgactagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc
    46441 aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta
    46501 cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg
    46561 cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc
    46621 ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct
    46681 ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg
    46741 atgtacaaag gcattcgctt tgatatcaac caccacataa ggaccgtgac gcgtgacatt
    46801 aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt
    46861 gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg
    46921 ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat
    46981 gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc
    47041 tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca aggtccacgc
    47101 cgcgtctttg cgctgcgcgg ttgtttcgaa atgcaccact tgcccggtac cgtgtacccc
    47161 tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc
    47221 cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt
    47281 actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga
    47341 cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg
    47401 ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca
    47461 ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcccctac ctgagtcggg
    47521 aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct
    47581 tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc
    47641 ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact
    47701 tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc
    47761 atattaaacg ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc
    47821 aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa
    47881 atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc
    47941 gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg
    48001 gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac
    48061 agtccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg
    48121 gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg
    48181 gcatcctgca cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta
    48241 cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg
    48301 gcttcgccca tagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg
    48361 gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag
    48421 attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac
    48481 tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc
    48541 gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc
    48601 gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc ggaccatgca
    48661 gcgtaatgcg gccaaactca ggcgcgggca gcaacgtatc cagcgcaacg tgttgttcat
    48721 gcccaataaa cttgctatag gcttcaaaca cttgcgtcgt gccacgcgct ttaccctcca
    48781 gggtatagcc tgcgatatgc ggcgtgccga tatccacttt tgtcagcaat tccacattaa
    48841 gttccggctc gccttcccag acatccagca ccacgcttaa cttctggcct tcattcaggc
    48901 aggtcagcag cgcggtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc
    48961 cgggcttcag gctacggatc agtttttcat ccgccagatg tagcgttttg tacggaccat
    49021 ctttaaagag tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac tcatccagcg
    49081 agcggaaatc accctcatcc ccacggtcgg cgcgaggcgg atcgcaaagt aaggttttaa
    49141 tccctaacgc ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg cccacgatcc
    49201 ccaccgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagc agggaggaga
    49261 aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg
    49321 cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtt ccaacaaatt
    49381 taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg
    49441 catccgcgtc tgccagttga gcgacgggga ttggacgccc gggaaccgcg atcacctcac
    49501 ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt
    49561 ttgtgttacc tgtatgagac gagagttaac cggacaagta tgccataatc tcgcggccag
    49621 gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct
    49681 cctcctgggc agccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct
    49741 ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg
    49801 caacaacaaa gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat
    49861 tcaccgctgc tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg
    49921 gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt
    49981 ctggggaatc atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg
    50041 ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag
    50101 atggttattc cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt accggcggag
    50161 cacaatgcgc taaaacagct ggtgaccaaa cttgcggcgg caacggggga acccagcaaa
    50221 cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg
    50281 aaactgttta accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat
    50341 acgccgacgc tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg
    50401 gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct
    50461 tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat
    50521 ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat
    50581 atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg
    50641 ctggttcgtt aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag
    50701 atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc
    50761 ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg
    50821 cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa
    50881 catccggctc cggttaacgc tgcgcccgct aatgcgaccc atgcacctgg ggcttgccag
    50941 agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca
    51001 aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg
    51061 gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc
    51121 ccgataaccg caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc
    51181 ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg
    51241 ccgttacagg cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa
    51301 ccgtaatggc tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc
    51361 gccattccgt tccatgacat cactttgcca gagtgttttg gccctacgat gcctaacccc
    51421 caggtcagag cgcctgtcag taactggctt tcaccaaacc caagaatcaa acgcccgata
    51481 accaacaggg caaatttgaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg
    51541 ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc gccatattga
    51601 tcggccagtc gcccggcgta accacgcgtc agcaccgtag ccagaaactg aatcccgacg
    51661 gcaatgccga ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc
    51721 ggcaacggca accctacggt catgtaggtg agaaaaaccg caaaagagat gcggaagagc
    51781 gaaaaactgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga
    51841 atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacggtgcg
    51901 ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc
    51961 tagaactggt ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt
    52021 tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga
    52081 cctactccgg cctactgcga attaatcttt tcagcttgcg cattaccagc gtggcgttgg
    52141 tgccgccgaa gccgaagctg ttagacataa cagtggtcag ttcgcgatcg gtcgtttcgg
    52201 tcacgatgtt cagacccgca gcctgctcgt ccagctcttc aatgttgatg ctcggggcga
    52261 taaagccgtg ttccagcatc agcagagagt agatagcttc ctgcacaccc gcagcgccca
    52321 gtgagtgacc ggtcatggct ttggttgcag aaatcgccgg gctcttatcg ccgaacactt
    52381 cacggatagc tgccagctct ttcacgtcgc caaccggagt cgaagtaccg tgagagttca
    52441 ggtaatcgat tggggtatca acgccatgca tcgccatctg catacaacgt actgcgcctt
    52501 cgccagacgg agcaaccatg tctgcaccat cagaggttgc gccgtagcca acgatttcag
    52561 catagatgta agcaccacgc gccagcgcgt gttccagctc ttcaaccact accataccgc
    52621 cgccgccagc gataacgaaa ccgtcacggt gagcgtcgta agtacgggag gctttttccg
    52681 gggtgtcgtt gtatttagta gatagcgcac ccatcgcgtc gaattcgcag gccatttccc
    52741 agcacagctc ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt tggatctgct
    52801 ctactgcgtt accgatacag tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa
    52861 cgccatgaat tttaaacggc gtggcgagac aggcagaaac gccggatgcc atcgctttag
    52921 tgaccacata cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga
    52981 acacctggaa acgcggggaa ccgccgccgg aacctgcaat caggccaacg cgcgggttat
    53041 tctggtaagc ttccggagag aggcccgcat cagcgattgc ctgctccata gaaaggaatg
    53101 cataaatgga tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg ccagtggtat
    53161 ccagttttat gttgccccag acgtggctac gcatgccgga atccttcagc tcctgagaga
    53221 aagtgatccc tgaacgtcct tcacgcagag atgccaggac ttcctgctgg ttattaccga
    53281 tgctggaaac aatgcccagg ccagtaatca ctgcacgttt cattcaatac ctctgtaagt
    53341 cgcacataga gtaagtttcg aatgcacaat agcgtacact tgtacgccga acaagtccga
    53401 tcagccattt aatagagata tttgcgcagc cttacacaca tcgctaagat cgagccaccg
    53461 cctgtaagac gagtaactta cgtgaaacac tactccatac aacctgccaa cctcgaattt
    53521 aatgctgagg gtacacctgt ttcccgagat tttgacgatg tctatttttc caacgataac
    53581 gggctggaag agacgcgtta tgtttttctg ggaggtaacc aattagaggt acgctttcct
    53641 gagcatccac atcctctgtt tgtgatagca gagagcggct tcggcaccgg attaaacttc
    53701 ctgacgctat ggaaggcatt tgatcagttt cgcgaagcgc atccgcaagc gcaattacaa
    53761 cgcttacatt tcattagttt tgagaaattt cccctcaccc gtgcggattt agccttagcg
    53821 catcaacact ggccggaact ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg
    53881 cccttgcccg gttgccatcg tttattgctc gatgaaggcc gcgtgacgct ggatttatgg
    53941 tttggcgata ttaacgaact gaccagccaa ctggacgatt cgctaaatca aaaagtagat
    54001 gcctggtttc tggacggctt tgcgccagct aaaaacccgg atatgtggac gcaaaatctg
    54061 tttaacgcca tggcaaggtt ggcgcgtcca ggcggcacgc tggcgacatt tacgtctgcc
    54121 ggttttgtcc gccgcggttt gcaggaagcc ggattcacga tgcaaaaacg taagggcttt
    54181 gggcgcaaac gggaaatgct ttgtggggtg atggaacaga cattaccact cccctgctcc
    54241 acgccgtggt ttaatcgtac gggcagcagc aaacgggaag tagcgattat cggcggtggt
    54301 attgccagcg cgttgttgtc gctggcgtta ttacggcgcg gctggcaggt agcgctttat
    54361 tgcgcggatg aagcccccgc gctgggtgct tccggcaatc gccagggggc gctgtatccg
    54421 ttattaagca aacacgatga ggcgctaaac cgctttttct ctaatgcgtt tacttttgct
    54481 cgtcggtttt acgaccaatt acccgttaaa tttgatcatg actggtgcgg cgtcacgcag
    54541 ttaggctggg atgagaaaag ccagcataaa atcgcacaga tgttgtcaat ggatttaccc
    54601 gcagaactgg ctgtagccgt tgaggcaaat gcggttgaac aaattacggg cgttgcgaca
    54661 aattgcagcg gcattactta tccgcaaggt ggttggctgt gcccagcaga actgacccgt
    54721 aatgtgctgg aactggcgca acagcagggt ttgcagattt attatcaata tcagttacag
    54781 aatttatccc gtaaggatga ctgttggttg ttgaattttg caggagatca gcaagcaaca
    54841 cacagcgtag tggtactggc gaacgggcat caaatcagcc gtttcagcca aacgtcgact
    54901 ctcccggtgt attcggttgc cgggcaggtc agccatattc cgacaacgcc ggaactggca
    54961 gagctgaagc aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa
    55021 catcattgta ttggtgccag ttatcatcgc ggcagcgaag atacggcgta cagtgaggac
    55081 gatcagcagc agaatcgcca gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa
    55141 gaggttgatg tcagtgataa agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat
    55201 ctgccaatgg taggcaatgt tcccgattat gaggcaacac tcgtggaata tgcgtcgttg
    55261 gcggagcaaa aagatgaggc ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg
    55321 gctttaggtt ctcgcggttt gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag
    55381 atgagcgacg aaccgattcc gatggatgcc agtacgctgg cggcgttaaa cccgaatcgg
    55441 ttatgggtgc ggaaattgtt gaagggtaaa gcggttaagg cggggtaatc tgctctcgca
    55501 gtgtttgccg gatgcggcgt gaacgcctta tccaacctac gtgtgacgtg taggcctgat
    55561 aagacgcgat aagcgtcgca tcaggcatgg tgctccagac gccgcaactt acagctgtga
    55621 cgcctgctga aacaggtttt cccacatatc ggtcaccagc gcctggtcac gcggcgacaa
    55681 ttcaccggca ccaatggctt tttccagact ctggctaacg gtcgtatgca ccgcctgagc
    55741 ggagtggtca tcaccacttt ccagttctgc gatggctaat gtcaggtggc cacgcaaata
    55801 cccactggca aacaactcat catcactggc atggtcaacc atgccgtcga ttaatgccag
    55861 aatgcgtgat tcaaactccg cgatcatctt ctttcctcat tgtaaaacgt ggcgctgact
    55921 tcagttgagc gcttccggcc acgggaactg ttccgccgta agttccggcg tgtgataata
    55981 attttgtagt gctttaatga agcgtgccgg gcgttccgga atgccatttg tcagataatc
    56041 catcacctgc gcatgtaccc gccgttgaaa caccacacga tccggttcga aatccccttc
    56101 cagattgtcg caactgacgt taaacggata tccagccgcc acgcagaata accagtcgaa
    56161 cgcctgcggc ttcacttcaa catcttcaaa ctggctttgc gtctgggcat cgcgtccgtc
    56221 cgggcaatac cagtagccga aatcaaccag ctcacggcgc gcttttccgg caatacacca
    56281 gtgcgaaatc tcatgaattg cgctggcata aaagccatga gcaaagacga ttcggttata
    56341 cggtacttcc gcatcagcag gaagatagat cggttcgtcg tcgcctttaa tcagacgggt
    56401 attaaattca tcggcaaagc agctattaaa aatttcaatc aactgctcgt agtggtgtgt
    56461 actgttcatt agttcatccc caaccagtgg aggatctcct gtccgtggct atcgtaaagt
    56521 aatttggcac tcatcaccgc cgagacgata acaatcatcg ggcggatcag cttttgtcct
    56581 ttgctcaaca ccaaccgtga acccatgcgc gcgccgagga actgccccac cagcatgaca
    56641 aagcccgtcg cccaaatcac tttgccgccg agaataaaca gcagcagacc gccaatgttt
    56701 gacgttgcgt tgagtaattt ggcgtgagcc gtggctttgg cgaggttgaa tccgcacagc
    56761 ataacgaagg ccagcgcgta aaacgatccg gcagccgggc caaagaatcc gtcataaaaa
    56821 ccgacgcagc caccggcaat taacgcgaag ggtaaaccgt acatccggcg ctggcgatct
    56881 tcttcaccca gctttggcat cagcaagaaa tagaggccga tacaaatcac cagaatgggc
    56941 aaaatctgcc gtaagacatc agcctgaacg tactgtacca acagtgcgcc gctcattgag
    57001 ccgacaaagg tcatggcaat attgagtttc tgatcgctta aactaaccac tttgcggcga
    57061 ataaagtaga tagtggcgga aatagagccg ccgcaggctt gcagtttatt ggttgccagc
    57121 gcgttagcgg gagacattcc cgcagccatc aatgccggaa tggtgagtaa cccaccgcca
    57181 ccggcaatcg agtcgataaa tcccgccagc atggcgacaa aaaagagaac tcccagcaac
    57241 agcggagaaa ccataaacag gctattaaac gtttccatta gatcacgtgc tcatccagta
    57301 gcgcctggca ggaaggcggc aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg
    57361 gttttggagg ttcaaaccag ctttgcagtt ctgccccgca accatcgcct gatggcggta
    57421 aaggttgatc ttcacactcc agactatcgg cagggcaacg taatcgtaca tgcatatgcg
    57481 cgcgatgctg gaaccagggt cgcactttgc gcaaccagtc gcgatcggtg cccgcatcaa
    57541 ggcaaagttg ttgtttaatc gccggattaa caaaaatgcg cgtgacgtct ttgtcctggg
    57601 cggcgagttt gatcaagctg aaaatttctg gcttccacag cgtggagaca acgtgtttac
    57661 cgtcgcggga aactaagtcc agtgcttgcg ggcgcaagag ctgcgcggag gtccagcgag
    57721 ttttcggcag ttgcagaaag atatcgacat ccagtccggt ctggtggctg gcatgaccgc
    57781 cgttgaaacg cccaccagcg ggcatcccca tatcgccaat cagcaccgta cccatgccca
    57841 gattgctcac ctggctactc agacgctgga taaacatcac cagatccggg tgaccgaaat
    57901 agcgacgctg atcggtacgc atgacctgat aatgttcgga ctgtatcggc aacgtgtcag
    57961 cgccgacaat acagccatta gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt
    58021 gggttatttt ttgccacggc gtcgctgcca ggctagcgct actggcaagc agagccagca
    58081 gcgcaatcgc ggttttgttc attttttacc agcgtggaat atcagtcttc acatcggcat
    58141 tttgcgcccg ctgccgtaac aggtgatcca ttaaaacgat cgccagcatc gcttcagcga
    58201 tcggcactgc acggatcccg acacagggat cgtgacggcc tttggtgatc atctcaactt
    58261 cttcgccaaa gcggttaata gtgcgccccg gcacggtaat gctggaggtc ggtttcagcg
    58321 ccatatgagc aatgatttgc tgcccgctgc tgataccgcc gaaaatgccg cccgcatggt
    58381 tgctctggaa accgtctttg gtgatttcgt cgcggttctg gctgccgcgc agcgccacta
    58441 cgtcaaaacc gtcgccaatt tccacgcctt tcaccgcgtt gatgctcatc agcgcatggg
    58501 cgatgtcggc atccaggcgg tcaaagaccg gctcgccaag tccggcgggg acaccactgg
    58561 caacaacggt gactttcgcg ccgatggagt cgccctcttt tttcagcgcg cgcatcagtt
    58621 catctaacgc gtcgattttg tcggggtccg ggcagaagaa cggattttgt tcaaccagcg
    58681 accagtcttt gatttccagc ggaatgtcgc ccatctgggt caggcagccg cggatttcaa
    58741 taccaaattt ctcggcgaga tattttttgg caatcgcccc tgccgccacg cgcatggcgg
    58801 tttcgcgggc ggaagaacgt ccaccgccgc gataatcgcg cagaccgtat ttttgttcgt
    58861 aggtgtaatc agcatggcct ggacggaaga cgtctttaat tgcgctgtaa tcctgagaac
    58921 gctggtcggt attttcgatc aacaagccaa tgctggtgcc agtagtaacg ccttcaaaaa
    58981 caccggagag tattttgacc tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc
    59041 cagggcgacg acgatcaagg tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg
    59101 gaacaccatc gacgatgcag ccgagcgcca gcccgtgcga ttcgccgaag gtggttacgc
    59161 gaaagagttg tccaattgtg tttccagcca tcacggctcc gttattgttg tgtttgcgtg
    59221 tttacttaat ctttataaat cgcgaaatgt tctcgtgcgg caataagctg ctctttagtg
    59281 agcataaaca caccatcgcc gccgttatca aactccagcc aggtgaacgg aacatcggga
    59341 tattgttcca taagatgtac catgctgttg cccacttcac aaatcaacac gccatcatca
    59401 gcaaggtaat ctgccgcgtt accgagaatg cgacgcgtca gtttcaggcc gtcggtgcca
    59461 gatgccaggc ccagttccgg ctcgtgacgg tattcgtttg gcaggtcgga catatcttcc
    59521 gcgtcgacat acggcgggtt agtgacaatc agatcgtatt gcactttcgg caaatcacgg
    59581 aacagatcgg aacgaatcgg aatgacgttg tggatcagac cgtgttcttc gatgttctgt
    59641 tcggcaaccg ccagcgcgtc gggagagata tctaccgcgt cgacttccgc ttccgggaag
    59701 gcataagcac aggcaatggc gatgcagccg ctaccggtac acatatctaa aatatgctgc
    59761 ggttgcttgc tgatgagtcc ggcaaattta ttgttgatca gttcaccaat cggcgagcgc
    59821 ggcaccagca cgcgttcatc gacgtaaaat tcatgaccgc agaaccaggc tttgttggtc
    59881 aggtaagcca ccggaatgcg ctcgttgacg cggcggatca cgcgttcaac aatacggtgt
    59941 ttttcgctgg aggtcagacg cgcggtgcgc atatcttccg ggatatccag cggcaggtag
    60001 agcgatggta acaccagctg tacggcttca tcccacgggt tatcggtgcc gtgaccgtac
    60061 cagatatttg ccgcgctgaa gcggcttacc gaccagcgca acatgtcctg aatggtttgc
    60121 agctcattta ctgcttcatc aacgaaaatt ttatccacgt attcctccag ggcatgatcg
    60181 caataatttc ggcggctagt ttgccatgaa gatgacgata aatcagcatt cacgcgcggt
    60241 gagtgaggaa aaatacgttt aaaacgatcg attgtgctac gagtcgggta aactgtagga
    60301 aaattagaaa tagagacaga taaatgaaaa agaaaacaac acttagcgag gaggaccagg
    60361 ctctgtttcg ccagttgatg gcggggactc gcaagattaa gcaggacacg attgtccacc
    60421 gaccgcagcg taaaaaaatc agcgaagtgc cggtgaaacg cttgatccag gagcaggctg
    60481 atgccagcca ttatttctcc gatgagtttc agccgttatt aaataccgaa ggtccggtga
    60541 aatatgttcg cccggatgtc agccattttg aggcgaagaa actgcgccgc ggcgattatt
    60601 caccggaatt gtttttggat ttacacggtc tgacgcagct gcaggccaag caggaactgg
    60661 gcgcgttgat tgccgcctgt cgccgcgaac atgtgttttg tgcctgcgtg atgtatgggc
    60721 acgggaagca tattttgaag caacaaacac cgctgtggct ggcacaacat ccgcatgtga
    60781 tggcatttca ccaggcaccg aaagagtacg gcggtgatgc ggctttgttg gtgttgattg
    60841 aagtggaaga gtggttgccg ccggagttgc cctgaatgct ggcagcaaat gccggagcag
    60901 cgtaaacgcc ttatccggcc tacaaatatg tgataattca ataaattgca attcacttcg
    60961 taggcctgat aagacgcatc agagcaatac tacgaacttc agttcattca atacatactg
    61021 aaaacgacac gtcgaatcaa tcggttgccc actactggta ttcacattca ccacaatatc
    61081 gtgtagtact ttatccgtta ccggatgacg agttggcgta accgttaccg ttgatttatc
    61141 acaaccgtaa gaggtaaagg ttaacaattc tgataccgcg cggcagtatg ctcctctgcc
    61201 atcattatca gaaaaattac tgccatcaca gagtctgccg ccttccatca ggtaggtcga
    61261 aagcgtggtg gtcactgttg accccgtgga ctgaattcgt aacgccttat tatggctgac
    61321 ataattttca ctccaggaga tatcgtatga agcgaccggt atcgttgggc atattgtgtt
    61381 atgcactgta ggggccgtat agcttccaaa tccgggtaaa tctgtatagg aataaaagga
    61441 tgcgatccaa gggcaaccat aatcatcaga tgcaacggta attttaaacg tcatatatgc
    61501 acaacgaaaa attttcgtat ctatgccagg gcaatttgag ttattaattg tatcaaagtc
    61561 agtcagtacc aaagttggag ctgaacccga cccagaccag gaaataaccc gcaacgtatc
    61621 gcctttatac gcaaaagaat catatttccc atatgcaacc tcctttggcg ttatcaccgt
    61681 aacattgaaa acactgctcg ctacagcacc ggttaccttg tccatggcat aataaactct
    61741 cacactgtca aaactcgcac tcataagttt atctccagca taactcttag atgcgccgac
    61801 aacactcaga acgagcagca ttaggctcgc gaaaattatg gtccattttt tcatttcaga
    61861 aatactcaaa ggttgccgtc actgtggcac taaagtctcc ggacgttaca tctcgcccgc
    61921 tctttgcctg taaccaggta ttaaaattta ggctgttatt tccctgattc agcgggagcg
    61981 tgacgcctgt tgtattgttg attggcaccg atgtgcctgc cgcagtttca ataccaatcc
    62041 cagcgccgga tgctgatgac gaggagtcaa aggccagaaa gccgggtaga gcactatctt
    62101 ctgttcctgt caacgttact ttgacattaa acaatgtaga actatgacaa tctttcaatt
    62161 gaaacaccaa tggcactcgc tctgactgcc caaggttcat taaatcactg gcggcaatgg
    62221 caggaaaatg tacttctgcc aactggctac cattaatcac tggcgtacac gatttactga
    62281 ttaaattgcc ggtaaagtgc aaattatctc ctgctattgt ggttccactg cccagcaaca
    62341 tcaggctact aataaaaaac aacgtacgct ttttcttcat tggtaatcta cctgtaacgt
    62401 ggcccaggcc tcaaagtccg cctctggcag aatgacacca ctctttttta ctggcacagc
    62461 cgttaacacc ggtaaatcgg tttcattcac cacaagtggc gtattgatcg taaaactctg
    62521 ccctgcctgt ttgagctgaa tgcctaaacc agtgatattg ctgcttactg cagcattatc
    62581 aaaatcgctt ggtgtgccac tccagctcaa ggtcatttcc attaatgggt cgcgaaccgc
    62641 cgtatcgcag gtaatggaat acggcacggt ttgctcataa ttgctgccat taatgtcatc
    62701 aataagcaca tcctgaaaag ttatttcaat ggtttgagcg ttgctaatcg tgcagttagg
    62761 cggctgcact aaataaccgt gaaaagagac gtttgagagt gccatcgagc aaaagcccca
    62821 cagcaaaata agcgagatcc gtttcattgg taatccacca caattgtggc actggcgtta
    62881 aattcagctg ccgctaacgt tgtaccactc tgtttcaccg gcacagcttc cagaacggga
    62941 actcccgagg aaaaattaaa tggcaaccat gagccagagg ttaagtcgag tattgtgtga
    63001 tcactacttt tctgaacccg aataccaaac ccaggaatac ccgtgctgat aactgtttca
    63061 ccgttaatca cgaccgttgt cgcctgcatc tgtagacgca ggtcatccat tgccagattg
    63121 ttacaaacaa gcgagtagtc aatgggacgt ttataatcaa ccctattgat cttattaatg
    63181 aatacattac caaactcaac tgccgcacca tttaccgtac aaggaggagg ggcatcgaca
    63241 agcacccgta aattcatact tactgactta ctcgctgcaa ctgcaaagcc gctaccaaca
    63301 caacatatca gtaacccagt taccgcccag acagaacaag atcctttcat actggctctc
    63361 ccggcctcag cctttgcggc tctgctcttc atcgacctag caactgttac cgttgcaagt
    63421 gaaaaagagc ggcatccgag cgccatagtc gttgacatag gtcagtaccg gcacgctacc
    63481 catcttcaca ttcagcggtg cagaggtttt cggcgacatc actattggcg agaaaccggc
    63541 tgccggattg ccatttttct gtgtgctggc attactgaaa atgacgtaat acgacgtcgg
    63601 gttattgacg gtaaattcct gaccgttgcg ggttaaagta atcttatatt gctaggggtt
    63661 tttcatgctg acgttctcca gcgccttcgg tcgccagaac aacttaatac gcgtttgtag
    63721 tgctatttgc agcgtgttgg ctttgtttga ttttggcggg atctcacgca cgttgaagta
    63781 gaacaggctt tcacgatcgg caggcaattt gttgatatcc ggcattccct gcacttttac
    63841 ctgcccgttc atcattgaat cgatgcgttg caccggtggc agaacagtta acggtgaact
    63901 gatcttgtta cccttttcat cttcgatcca gctttgggcc agataaggca gtttcggatc
    63961 gttattgcgc aacgtcacgc tgattgattt gtcgctttca ttaaatatca aacgggtacg
    64021 atctggcgtt acagaagcct gggcagacaa actggcagcg ctaagcagta acgctagcgt
    64081 cgtagcaccc aatttggcag agcaaagtaa atcagacatg gatcattcct taatcacgtt
    64141 attggttagc tgaaacagaa acagggggat tggtgggtgt cacctgttgc gtctgctcct
    64201 gaatgacagg ctttatgtca tcaggaacaa ccggcgcgac gttgccttta tgctggcatg
    64261 gcagtaacaa gccgttaaac agatcggcag gcagcgggtc cggcaggttg atatcgcaat
    64321 gcgcaacacc actccagaac acactcatgt gttcgccagg tttcacccca gccagataaa
    64381 cattgccgtc atcatcgaca aggcccactg tctgctcgtt atcatttttt acttctgcgc
    64441 caaacggagg atggctgccg tcttgtaaac gcagcaccgc catcgctttt tgaccactga
    64501 tgacggcaaa tttgcggtag ccaatcgccc cttcagttag cgtggcttgt accaccgact
    64561 gggttgcttc ggcgttttcc ggcagtttgt tgaggtcgat atacgcctga ttgcggtaat
    64621 agttattgac gtcagaaacg acggctttac caaacatatt ggtataaaca gcagcaccgt
    64681 taccttctac cggaacatcg gcaacgccat cggcatcaat caacaagcgt gtcccgccca
    64741 tattctgggt acggtgaagt gcgccaccgt gggcagtaag cgttgcgccg ccctgtaacg
    64801 acaagcccgc agaggtgtat tgcccttcat ggtagttcgc actgaggtca acctgcgcca
    64861 gcgaaccatc atggctgtaa tagccgtcaa cgctggtgtg tttgtcactg gtgccgacgt
    64921 tcaactgata gtgagtcgcg tcatcgacac ggctgaaata accgacctga ctgctgtccg
    64981 tcccactgcc atagtaccgt tatagctaac ggtactgttg tcgccccacg gcatactgag
    65041 cgaaatgtac atgcctttgt cggcctggtt gtcatactcg taacggtagc cagtcatcga
    65101 gatgctgaca ttacgaatac tgcccatatt gaaatagtgc gagagcatga tgttgtagtt
    65161 tgtctgctcc tcgcgatccc agtaggtatg gcgggtatag ttgagataaa ccgaaacacc
    65221 cgcatcgcgg aagttctggt tataagtggc ggtgtacatc tctttgtcgt tgcccgtgcg
    65281 taccattccg ctgtcactgg catccagata ctcgctcatg gtcataaagt tctcttccga
    65341 gaagcgatat ccagcaaaag taacgcggct gttgagctgg tcaaagtctt tggaataact
    65401 cacacggaag gagttaccgt ccagcgaacc tttgccataa gcggtgtctt tatccagttt
    65461 ggtgtgcgag tgagtaacat caaacgcgac cgcgccgaat gtagacaaat cgcgaccgac
    65521 accaagcgcc gcagactgat agttttcatc tcccagtgcg ccgccatata acgaccagcc
    65581 gttagcgatc ccccaggaag cttcagcacc agaaaaaaat tcaccctcga catggtgtcc
    65641 ccactcttgc ggacggccca tcatgatctt atagcgaacc tgacctggac gagtgaggta
    65701 tggcatcgag gcggtgctga tgtcatattc ctgcacctgg ccgttctgtt cttcaatgcg
    65761 aatatgcaac gtaccggaga cagaatcacc aagatcctga atacgaaacg gtccagccgg
    65821 aacctgcgtt tcgtaaatca cacgccccat ctggctgacg gtcacttttg ctgtggtgtg
    65881 tgccacgccg gaaatgtctg gcgcgtagcc gcgcagattg ggaggcaaca tttgatcgtc
    65941 agtactgaca ctgccaccaa catagttaaa accatcaaaa atatcggatc tgaggtaatc
    66001 ctcgcccagc gccagtttgg cttttaatga tggtaacgcc cgcctggcat agtagcgact
    66061 ccactcccat tttttttgag tttcatcgcc gctgaattca tcgtcatcat tactgcgagt
    66121 atgttgatag ttagtctgcc agtcagcacg catacgccac ggccctaggt taaccccaac
    66181 cgtcccgttg ccgctgatct cgttactatc atcaccgcca ttttcttcgt gccgggtttg
    66241 tgcgttgatg ctgtagtccg cgacgatccc ggagatgccg tcatcccaac gtgaaggcgg
    66301 atcccagtcg ggataagtat attcgaggta agcctgtggc agtgaaatga ctaatgcgga
    66361 ctggcttaaa tcagccttaa tttccatgcc ttccagttga ccggatttca ggcatttagc
    66421 atcgtggctc cattgcagat ttttcgccac gtcttctttt aaaccaaact gcgctaccag
    66481 ttccggtgtc agacaagcat agcttttgct cgcgtcatct tcaccagcat accagtaaat
    66541 atcgtactct tccgccaatg gctgtttatt tagttgaacc tgtaaattgt attttccggg
    66601 ctcaacatat ccctggctgg aaaaacgctt cagatcaatt tttgtgtcgc cttttaattc
    66661 cagaaaacgg gaatcaaact gaatgtcgtc ttcagcccag acactgctat aactccctga
    66721 cattgccagc gcaatgcacc acggaagaat ccgtaatcga aaaagagaat ggtcaggcat
    66781 acacgtttct atccgttttt attcttgttc ggtcaaaata catttaaatc ccccatagca
    66841 gtgtcgctat gggggtatgc cttacaggta agtaatctgg aaggtggtat ttgcttcaaa
    66901 attacccaga tctggcgcat cagctgcgcc gaccagccag gcgttaaagt tcagtgtctg
    66961 cttcgctttg cctttgttgg tagaagtgtc tttcactatt ttctggtcaa tacccatgcc
    67021 gcttttgtaa gaggtaccca gagagtcacc gatcgccagg ctaacattgt taaatgccgc
    67081 accggtatcg gtattgaaaa tggtgtaata attgccgcta tttgcagaag aaacggtacc
    67141 agtaaaggta gtggtcattg tttcctgagt atcaaatacg caatcttgca gacgaatctg
    67201 gaaacttttc ggcgtggtgg tgccgttatt tttcagtttt tcggcaccga tatcacccat
    67261 atcaacttcc agtttgtgat cgtctggaac gatagaacac actgagtctt caatggtgcc
    67321 gtaaaaacgt gcagtaccgt tgttacccgc agcgattgtt gccgtagagg tgaacacgcc
    67381 catcaccatt gccgcagcaa tagctgtttt aacaaactta ctcattattt gtccttaaac
    67441 tataaataat taaaaatata aacattgcaa tacattgatt cagtcaatag ccaat
//
LOCUS       DAOFMX010000008        66899 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.008, whole genome shotgun sequence.
ACCESSION   DAOFMX010000008 DAOFMX010000000
VERSION     DAOFMX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.008"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(66..1070)
                     /gene="cra"
                     /locus_tag="QBA20_000657"
     CDS             complement(66..1070)
                     /gene="cra"
                     /locus_tag="QBA20_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDQ6291308.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1153..1239)
                     /locus_tag="QBA20_000658"
     CDS             complement(1153..1239)
                     /locus_tag="QBA20_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDQ6291309.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1251..1742)
                     /gene="ilvN"
                     /locus_tag="QBA20_000659"
     CDS             complement(1251..1742)
                     /gene="ilvN"
                     /locus_tag="QBA20_000659"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDQ6291310.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1745..3469)
                     /gene="ilvI"
                     /locus_tag="QBA20_000660"
     CDS             complement(1745..3469)
                     /gene="ilvI"
                     /locus_tag="QBA20_000660"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDQ6291311.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3588..3632
                     /locus_tag="QBA20_000661"
     CDS             3588..3632
                     /locus_tag="QBA20_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291312.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3787..4731)
                     /gene="leuO"
                     /locus_tag="QBA20_000662"
     CDS             complement(3787..4731)
                     /gene="leuO"
                     /locus_tag="QBA20_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDQ6291313.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5391..5477
                     /gene="leuL"
                     /locus_tag="QBA20_000663"
     CDS             5391..5477
                     /gene="leuL"
                     /locus_tag="QBA20_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDQ6291314.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5570..7141
                     /gene="leuA"
                     /locus_tag="QBA20_000664"
     CDS             5570..7141
                     /gene="leuA"
                     /locus_tag="QBA20_000664"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDQ6291315.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7141..8232
                     /gene="leuB"
                     /locus_tag="QBA20_000665"
     CDS             7141..8232
                     /gene="leuB"
                     /locus_tag="QBA20_000665"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDQ6291316.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8235..9635
                     /gene="leuC"
                     /locus_tag="QBA20_000666"
     CDS             8235..9635
                     /gene="leuC"
                     /locus_tag="QBA20_000666"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDQ6291317.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9646..10251
                     /gene="leuD"
                     /locus_tag="QBA20_000667"
     CDS             9646..10251
                     /gene="leuD"
                     /locus_tag="QBA20_000667"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDQ6291318.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10300..11274)
                     /locus_tag="QBA20_000668"
     CDS             complement(10300..11274)
                     /locus_tag="QBA20_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6291319.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALARVELPSENALSMQGDWQDSNVRMLFVASTLMW
                     TCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVI
                     AVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLF
                     TNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV"
     gene            complement(11376..11507)
                     /gene="sgrT"
                     /locus_tag="QBA20_000669"
     CDS             complement(11376..11507)
                     /gene="sgrT"
                     /locus_tag="QBA20_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDQ6291320.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11596..13251
                     /gene="sgrR"
                     /locus_tag="QBA20_000670"
     CDS             11596..13251
                     /gene="sgrR"
                     /locus_tag="QBA20_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDQ6291321.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13285..13384
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13415..14398
                     /gene="thiB"
                     /locus_tag="QBA20_000671"
     CDS             13415..14398
                     /gene="thiB"
                     /locus_tag="QBA20_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDQ6291322.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14374..15984
                     /gene="thiP"
                     /locus_tag="QBA20_000672"
     CDS             14374..15984
                     /gene="thiP"
                     /locus_tag="QBA20_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDQ6291323.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            15968..16666
                     /gene="thiQ"
                     /locus_tag="QBA20_000673"
     CDS             15968..16666
                     /gene="thiQ"
                     /locus_tag="QBA20_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDQ6291324.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16780..17544)
                     /locus_tag="QBA20_000674"
     CDS             complement(16780..17544)
                     /locus_tag="QBA20_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ6291325.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17630..18508)
                     /gene="araC"
                     /locus_tag="QBA20_000675"
     CDS             complement(17630..18508)
                     /gene="araC"
                     /locus_tag="QBA20_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDQ6291326.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            18847..20547
                     /gene="araB"
                     /locus_tag="QBA20_000676"
     CDS             18847..20547
                     /gene="araB"
                     /locus_tag="QBA20_000676"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDQ6291327.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSMVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20558..22060
                     /gene="araA"
                     /locus_tag="QBA20_000677"
     CDS             20558..22060
                     /gene="araA"
                     /locus_tag="QBA20_000677"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDQ6291328.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22260..22955
                     /gene="araD"
                     /locus_tag="QBA20_000678"
     CDS             22260..22955
                     /gene="araD"
                     /locus_tag="QBA20_000678"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDQ6291329.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23030..25381
                     /gene="polB"
                     /locus_tag="QBA20_000679"
     CDS             23030..25381
                     /gene="polB"
                     /locus_tag="QBA20_000679"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDQ6291330.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25546..28452
                     /gene="rapA"
                     /locus_tag="QBA20_000680"
     CDS             25546..28452
                     /gene="rapA"
                     /locus_tag="QBA20_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDQ6291331.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28464..29123
                     /gene="rluA"
                     /locus_tag="QBA20_000681"
     CDS             28464..29123
                     /gene="rluA"
                     /locus_tag="QBA20_000681"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDQ6291332.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29240..30055)
                     /gene="djlA"
                     /locus_tag="QBA20_000682"
     CDS             complement(29240..30055)
                     /gene="djlA"
                     /locus_tag="QBA20_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDQ6291333.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30310..32664
                     /gene="lptD"
                     /locus_tag="QBA20_000683"
     CDS             30310..32664
                     /gene="lptD"
                     /locus_tag="QBA20_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDQ6291334.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32717..34003
                     /gene="surA"
                     /locus_tag="QBA20_000684"
     CDS             32717..34003
                     /gene="surA"
                     /locus_tag="QBA20_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDQ6291335.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34003..34992
                     /gene="pdxA"
                     /locus_tag="QBA20_000685"
     CDS             34003..34992
                     /gene="pdxA"
                     /locus_tag="QBA20_000685"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDQ6291336.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            34989..35810
                     /gene="rsmA"
                     /locus_tag="QBA20_000686"
     CDS             34989..35810
                     /gene="rsmA"
                     /locus_tag="QBA20_000686"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDQ6291337.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            35813..36190
                     /gene="apaG"
                     /locus_tag="QBA20_000687"
     CDS             35813..36190
                     /gene="apaG"
                     /locus_tag="QBA20_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDQ6291338.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36197..37039
                     /gene="apaH"
                     /locus_tag="QBA20_000688"
     CDS             36197..37039
                     /gene="apaH"
                     /locus_tag="QBA20_000688"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDQ6291339.1"
                     /translation="MATYLIGDVHGCYDELSALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37117..37596)
                     /gene="folA"
                     /locus_tag="QBA20_000689"
     CDS             complement(37117..37596)
                     /gene="folA"
                     /locus_tag="QBA20_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDQ6291340.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37787..39649)
                     /gene="kefC"
                     /locus_tag="QBA20_000690"
     CDS             complement(37787..39649)
                     /gene="kefC"
                     /locus_tag="QBA20_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDQ6291341.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39642..40172)
                     /gene="kefF"
                     /locus_tag="QBA20_000691"
     CDS             complement(39642..40172)
                     /gene="kefF"
                     /locus_tag="QBA20_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDQ6291342.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40220..41614)
                     /locus_tag="QBA20_000692"
     CDS             complement(40220..41614)
                     /locus_tag="QBA20_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6291343.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41673..41960)
                     /gene="fixX"
                     /locus_tag="QBA20_000693"
     CDS             complement(41673..41960)
                     /gene="fixX"
                     /locus_tag="QBA20_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDQ6291344.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(41957..43243)
                     /locus_tag="QBA20_000694"
     CDS             complement(41957..43243)
                     /locus_tag="QBA20_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDQ6291345.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43294..44235)
                     /locus_tag="QBA20_000695"
                     /pseudo
     CDS             complement(43294..44235)
                     /locus_tag="QBA20_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44250..45020)
                     /locus_tag="QBA20_000696"
     CDS             complement(44250..45020)
                     /locus_tag="QBA20_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDQ6291346.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45492..47006
                     /gene="caiT"
                     /locus_tag="QBA20_000697"
     CDS             45492..47006
                     /gene="caiT"
                     /locus_tag="QBA20_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDQ6291347.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47037..48179
                     /gene="caiA"
                     /locus_tag="QBA20_000698"
     CDS             47037..48179
                     /gene="caiA"
                     /locus_tag="QBA20_000698"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDQ6291348.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48308..49525
                     /gene="caiB"
                     /locus_tag="QBA20_000699"
     CDS             48308..49525
                     /gene="caiB"
                     /locus_tag="QBA20_000699"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDQ6291349.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49599..51152
                     /gene="caiC"
                     /locus_tag="QBA20_000700"
     CDS             49599..51152
                     /gene="caiC"
                     /locus_tag="QBA20_000700"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDQ6291350.1"
                     /translation="MDIIGGQHLRQIWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51261..52046
                     /gene="caiD"
                     /locus_tag="QBA20_000701"
     CDS             51261..52046
                     /gene="caiD"
                     /locus_tag="QBA20_000701"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDQ6291351.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52052..52642
                     /gene="caiE"
                     /locus_tag="QBA20_000702"
     CDS             52052..52642
                     /gene="caiE"
                     /locus_tag="QBA20_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDQ6291352.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52728..53123)
                     /gene="caiF"
                     /locus_tag="QBA20_000703"
     CDS             complement(52728..53123)
                     /gene="caiF"
                     /locus_tag="QBA20_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDQ6291353.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53385..56606)
                     /gene="carB"
                     /locus_tag="QBA20_000704"
     CDS             complement(53385..56606)
                     /gene="carB"
                     /locus_tag="QBA20_000704"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDQ6291354.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLALSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56624..57772)
                     /gene="carA"
                     /locus_tag="QBA20_000705"
     CDS             complement(56624..57772)
                     /gene="carA"
                     /locus_tag="QBA20_000705"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDQ6291355.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58228..59049)
                     /gene="dapB"
                     /locus_tag="QBA20_000706"
     CDS             complement(58228..59049)
                     /gene="dapB"
                     /locus_tag="QBA20_000706"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDQ6291356.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59216..60130)
                     /gene="rihC"
                     /locus_tag="QBA20_000707"
     CDS             complement(59216..60130)
                     /gene="rihC"
                     /locus_tag="QBA20_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDQ6291357.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60196..61146)
                     /gene="ispH"
                     /locus_tag="QBA20_000708"
                     /gene_synonym="lytB"
     CDS             complement(60196..61146)
                     /gene="ispH"
                     /locus_tag="QBA20_000708"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDQ6291358.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61148..61597)
                     /gene="fkpB"
                     /locus_tag="QBA20_000709"
     CDS             complement(61148..61597)
                     /gene="fkpB"
                     /locus_tag="QBA20_000709"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDQ6291359.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61684..62178)
                     /gene="lspA"
                     /locus_tag="QBA20_000710"
     CDS             complement(61684..62178)
                     /gene="lspA"
                     /locus_tag="QBA20_000710"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDQ6291360.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62178..64994)
                     /gene="ileS"
                     /locus_tag="QBA20_000711"
     CDS             complement(62178..64994)
                     /gene="ileS"
                     /locus_tag="QBA20_000711"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDQ6291361.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65037..65978)
                     /gene="ribF"
                     /locus_tag="QBA20_000712"
     CDS             complement(65037..65978)
                     /gene="ribF"
                     /locus_tag="QBA20_000712"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDQ6291362.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(65986..66204)
                     /locus_tag="QBA20_000713"
     CDS             complement(65986..66204)
                     /locus_tag="QBA20_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDQ6291363.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66307..66570
                     /gene="rpsT"
                     /locus_tag="QBA20_000714"
     CDS             66307..66570
                     /gene="rpsT"
                     /locus_tag="QBA20_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDQ6291364.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16229 a        18199 c        17227 g        15244 t
ORIGIN      
        1 gatgtccagt cccgtactct acgcgccaga gggaaattca cctggcgcgt atttttgttc
       61 gcggattagc tacggctgag cacgccgcgg cgatagagat tacgtttaat gcgcgttaaa
      121 ccaggttttg gcttacgcgg ttcgtccagg cttgccagga caatctccag cacacgctct
      181 gcgacatcgc ggtgacgttg agccactgcc agcaccggac actgtaagaa gtcgagcagt
      241 tcgttatcgc caaaggtggc aattgccagg tcagaaggca gtttgccgtc gcgacgcagc
      301 gtgacatcca tcactccttg caacaacgca aacgacgttg tgaacagcgc ctgcggcatc
      361 ggatgcgttt ccagccattt ttcgaataac tgggcagccg cctcccgctc atagctgttg
      421 gcatacagga aatgcacttc gcgcggatca tctttccaag cagtacggaa accttgttca
      481 cgcaggaagc tgacagaaag ctccggcagc gcgccaagat aaagcaccgt ctcggcggga
      541 aacttacgta actcttccgc cagcatttcg gcatcatcct gatcggcacc aaccacgctg
      601 gtgaaatgtt cacgatcgag ggcgcggtcc agcgcgacaa tcgggaacgg gtcgttagcc
      661 cagcgttgat aaaaaggatg ctcaggtggc aacgacgtcg aaacaataat ggcatcaacc
      721 tgacgctgta aaaggtgctc aatgcagcgc atttcgttgt ctggctgatc ttctgagcag
      781 gcaatcagca gttgataacc ccgttgccgc gcctggcgtt caagatagtt agcgatgcgg
      841 gtatagctgg tgttctccag atcggggatc acaagaccaa tagaacgtgt gcgtccagca
      901 cgaagcccag ctgccacggc gttcgggtgg taattgtgct cacgcaccac agccatgact
      961 ttttcaacgg ttttgtcgct cacacggtat tgcttcgctt tgccgttaat aacatagctt
     1021 gcagtggtcc gcgacactcc cgccagccga gcgatttcat ccagtttcac aattgcccct
     1081 tgcgtaaaat gtaaaaaacc atagccattg tactggtatg ggttaaattc attaacatct
     1141 aagcgcagaa aattaactgc ggcaactgct tttattccag gttcccactg atttcgcaaa
     1201 aaaagcccaa cgtgacatgt tgggctgcaa attgcgcact gagatcattc tcaacgcatt
     1261 attttatcgc cgcgcgaaag tccgaccaca ccagagcgag caacttccac aattttcgcc
     1321 acatcgcgaa tcgatgctaa aaatgcatca agcttatcgc tggtgcctgc taattgaacg
     1381 gtataaagcg agggggtgac atcgataatt tgcccacgga atatttccgt attacgtttc
     1441 acttcgtcac gcccgtaacc gctggcctga attttcacca gcatgatttc ccgctcaaca
     1501 tgcgcgccct gccccaactc actcacgcgc aagacatcga ccagcttgtg taattgcttt
     1561 tcgatctgct caagtacttt ttcatcgccc acggtctgga tggtcatacg cgataatgtc
     1621 ggatcgtcgg ttggcgcaac ggtcaggctt tcaatgttgt agcctcgctg ggaaaaaagg
     1681 ccaatcacgc gggataacgc gcctgattca ttttcgagta agactgataa tatccggcgc
     1741 ataatcaggt tctctccgtt ttgcttaacc acatttcatc cattccgccc ccgcgaatct
     1801 gcatcgggta gacgtgctca ctgccatcga cggtaacatc aacaaacacc aggcgattat
     1861 tgcgcacctg ttccagcgcc tcgctaagtt tgctttccag ctcatgcgga tgagagatct
     1921 ggatcccgac gtgaccatag gcttccgcca gacggacgaa atcgggtagt gattgcatat
     1981 aagattgtga atgacggccg gaatagatca tgtcctgcca ctgtttcacc atccccagat
     2041 agcggttatt gagattcacc accagtacgg gcaactcgta ttgcaacgcg gtagacagtt
     2101 cctggatgtt catctgaata ctgccgtcgc cagtgacgca aaccacggtt tcttctggca
     2161 acgccatttt gacgcccagt gccgcaggta aaccaaaacc catcgtgccg agaccgccgg
     2221 aattgatcca gcgacgcggt ttgtcgaatg gataataaag cgcagcaaac atctggtgct
     2281 gcccgacatc ggacgtcacg taagcgtccc cattagtcaa ccgccaaaga gtctcgatca
     2341 ccgcctgcgg tttaatcttt tcactgtgag tgtcatattt caggcactga agagcgcgcc
     2401 actgttcaat ctgctgccac cagtcgcgga tctcatccag cggttgatgg gcggattctt
     2461 gcgacaggag ttcaagcatt tgttcgagga cctggcgagc atcccccaca atcgggatat
     2521 ccgcagtcac ggttttagaa atggaagtag gatcaatatc gatatgcaga acagtggcat
     2581 ttgggcagta ctttgccaga ttgttcgtcg ttcggtcatc aaatcgtacc ccgacggcga
     2641 aaatcacatc cgcgttatgc atcgtcatat tggcttcgta ggtaccgtgc attcccagca
     2701 tgcccagtgc ctgacgatgc gttgccggaa acgcccccag ccccatcaat gaggaaacaa
     2761 cgggcagatt caacgcctcc accgtttctt tcaactgctg atggcagccc gccatgattg
     2821 ccccaccgcc tacgtagaca accggttttt ttgccgctac cagcgtttgc agagcacgct
     2881 taatttgccc tttatgtccg gtagtagtgg gattgtaaga acgcatactg accgactcag
     2941 ggcagacata gggtaatttg ttcgccggat taagaatatc tttcggtaaa tcaacgacca
     3001 ctggtcctgg gcgaccactt gccgccagcc agaaagcctt tttcagcacc tgcggaatgt
     3061 cttccgtttg cttaaccaga aaactgtgtt taaccaccgg tcgcgaaatc cccaccatgt
     3121 cgcactcctg aaaggcatcg taacctatca acgaggtcgc tacctgcccg gaaaggacaa
     3181 ctaatggaat ggaatccata taagcggtgg cgatgccggt aatcgcattg gtcgcccctg
     3241 gacccgacgt taccagcacg acgccgactt ccccggtcgc acgcgccagg ccatcggcca
     3301 tatgcaccgc cgcctgctca tgacgaacta atacatgatc aataccaccc acggtatgca
     3361 atgcatcgta aatatcaagg actgcgcctc cgggataacc gaatacttgt ttaacgccct
     3421 gatcgataag cgatcggacg accatctcgg ctccagacaa catctccatg gcctgcctca
     3481 ctgtttgacg gaaaaaatgt gtaaaacaca ttaaccgtcc gatcttgctt aagcaattgg
     3541 cagccagaga cataaacgca gggtaataag aataaacagg aggaaaggtg aaaagaaacg
     3601 ataaaatcct ccattacgaa ggattaaatt gataataaat cggctgaatc ccacaactta
     3661 cagcaatttc tctatttttt taattaatcc taaaatagag agtccacaca ttcagaataa
     3721 aattctacat tcaataaaaa atgcagaata aaccagacat tcatgtctgg cctattctgc
     3781 attcagttag cgtttgcaaa ttgagactaa ttgttcttcc atccactgat ggcctttatc
     3841 gcgcccggca gcttcatgcc aagagagata acaggttctg ctgttttgtt ttaacggcag
     3901 cggtaatacc tgtaattcta aggattcagc gaactcttca gccagccaac gcggcgcaat
     3961 agcgaccaaa tgcgtttgcg acaccacgct aagtacgctc atcattgcca tgccctgata
     4021 cgcgatactg gcttgcttat ctaccgtgtc ataccaaggt tgactaaatg acgcgaaacg
     4081 atcgagcgaa accgccgcat gttgttcgtt ataaacatca tgtttcagta acgggccttt
     4141 aattgttgga tgatttttgc tggctaccag caccatttca tctttaaata atggcacgct
     4201 ggtaaattca ggacgatgga agtcttcata actaatcaca aactccgttt cctgataacg
     4261 cagctgatgt tcagtgttct gatttaatga agacttgaac ataacatgta tatttggcgc
     4321 aatctgctca atgtgattat aaatctgcga ggtcagaatg ctgtctaacg ggctgcaaac
     4381 acaaagatga aatacacgtt cactgctcgc gggttcaaaa cctgaaccag gcaattcatt
     4441 ttgtactagt tgcaatgcct ggcgaactga accaaaaagt tgaaatgcgc gagcagtcgg
     4501 ttgaatacca cggccataac gaacaaaaag ctcgtcatta aacatcacct tcaggcgtgc
     4561 aacagcgtta ctgaccgcag gttgcgacat acccagaaca tgagcggcac gggtaatatt
     4621 ttgctcctgc atcacggcat cgaaaacggt taataagttg agatcgacca tgcgtagctg
     4681 tggcttgctt aactccgccg tctctgaatg atctgtttgt acctctggca tacttaactc
     4741 cactgtcaca cttaactccc tttcccttat tggaatgcag agaaataatc ctgaagaata
     4801 tgatttatca tgcatataaa ataagaaaaa gcaaaatgag taaaattcgg aaaacataaa
     4861 gatacgggtt ctgataaaaa cagaatccat tgccgtggaa tgagtcattt accacagcat
     4921 aataatccat aatgtttttg ttgcgaaaac aatctaatca taactaccgc gaatactcaa
     4981 tcatctacaa aatggattaa atgtgcttat ttaataatta atttatatat ttaatgcatt
     5041 aattcttaac attaattgat caataatatt caccaaatca atatcaaaaa aaatcgcaaa
     5101 acatataatt caatacaaat catcaggata ggttttgcaa cgcgtgcatt ttgtcccctt
     5161 tttcctcgtt gattagatgc aaaaatttat gctgaaatat gtcaaccgat gaaaagcgtc
     5221 ggtagttaag cagaaattaa tatcgcttac tttaaccacc gtagcacaat tagctaattt
     5281 tacggatgca gaactcacgc tggcgggacg tttttattgc gtcagggttg acatccgttt
     5341 ttgtatccag taactctaaa agcatatcgc attcatctgg agctgattta atgactcaca
     5401 tcgttcgctt tatcggtcta ctactactaa acgcatcttc tttgcgcggt agacgagtga
     5461 gcggcatcca gcattaagcc agcacgcagt caaacaaaaa acccgcgcca ttgcgcgggt
     5521 ttttttatgc ccgaagcgag gcgctctaaa agagacaagg acccaaacca tgagccagca
     5581 agtcattatt ttcgatacca cattgcgcga cggtgaacag gcgttacagg caagcttgag
     5641 tgtgaaagaa aaactgcaaa ttgcgctggc ccttgagcgt atgggtgttg acgtgatgga
     5701 agtcggtttc cccgtctctt cgccgggcga ttttgaatcg gtgcaaacca tcgcccgcca
     5761 ggttaaaaac agccgcgtat gtgcgttagc tcgctgcgtg gaaaaagata tcgacgtggc
     5821 ggccgaatcc ctgaaagtcg ccgaagcctt ccgtattcat acctttattg ctacctcccc
     5881 aatgcacatc gccaccaagc tgcgcagcac cctagacgag gtgatcgaac gcgctatcta
     5941 tatggtgaaa cgcgcccgta attacaccga tgatgttgaa ttttcttgcg aagatgccgg
     6001 gcgtacaccc attgccgatc tggcgcgagt ggtcgaagcg gcgattaatg ccggtgccac
     6061 caccatcaac attccggaca ccgtgggcta caccatgccg tttgagttcg ccggaatcat
     6121 cagcggcctg tatgaacgcg tacctaacat cgacaaagcc attatttccg tacataccca
     6181 cgacgattta ggcctggcgg tcggcaactc actggcggcg gtacatgccg gtgcacgcca
     6241 ggtggaaggt gcaatgaacg ggatcggcga gcgtgccggt aactgttcgc tggaagaagt
     6301 catcatggcg attaaagttc gtaaggatat tctcaacgtc cacaccgcca ttaatcacca
     6361 agagatatgg cgcaccagcc agttagttag ccagatttgt aatatgccga tcccggcaaa
     6421 caaagccatt gttggcagcg gcgcattcgc tcactcctcc ggtatccacc aggatggcgt
     6481 gctgaaaaac cgcgaaaact acgaaatcat gacaccagaa tctattggtc tgaaccaaat
     6541 ccagctgaat ctgacctctc gttcggggcg tgcggcggtg aaacatcgca tggatgagat
     6601 ggggtataaa gaaagtgaat ataatttaga caatttgtac gatgctttcc tgaagctggc
     6661 ggacaaaaaa ggtcaggtgt ttgattacga tctggaggcg ctggccttca tcggtaagca
     6721 gcaagaagag ccggagcatt tccgtctgga ttacttcagc gtgcagtctg gctctaacga
     6781 tatcgccacc gccgccgtca aactggcctg tggcgaagaa gtcaaagcag aagccgccaa
     6841 cggtaacggt ccggtcgatg ccgtctatca ggcaattaac cgcatcactg actataacgt
     6901 cgaactggtg aaatacagcc tgaccgccaa aggtcacggt aaagatgcgc tgggtcaggt
     6961 ggatattgtc accaactaca acggtcgccg cttccacggc gtcggcctgg ccaccgatat
     7021 tgtcgagtcc tccgccaaag ccatggtgca cgtactgaac aatatctggc gtgccgcaga
     7081 agtcgaaaaa gagttgcaac gcaaaactca acacaacgaa aacaacaagg aaaccgtgtg
     7141 atgtcgaaga attaccatat tgccgtattg ccgggagacg gtattggtcc ggaagtgatg
     7201 acccaggcgc tgaaagtgct gaatgccgtg cgcaaccgct ttgcgatgcg catcaccacc
     7261 agccattacg atgtaggcgg cgcagccatt gataaccacg ggcaaccact gccgcctgca
     7321 acggttgaag gttgtgagca agccgatgcc gtgctgtttg gctcggtagg cggcccgaag
     7381 tgggaacatt taccaccaga ccagcaacca gaacgcggcg cgctgctgcc gctgcgtaag
     7441 cacttcaaat tattcagcaa cctgcgcccg gcaaaactgt atcaggggct ggaagcattc
     7501 tgtccgctgc gtgcagacat tgccgctaac ggcttcgaca tccagtgcgt gcgcgaactg
     7561 accggcggca tctatttcgg tcagccaaaa ggccgcgaag gtagcggaca atatgaaaaa
     7621 gcgtttgata ccgaggtgta tcaccgtttt gagatcgagc gtattgcccg catcgcgttt
     7681 gaatctgctc gtaaacgtcg ccacaaagtc acctcaatcg acaaagctaa cgtgctgcaa
     7741 tcctctattt tatggcgtga aatcgtcaac gagatcgcca cggaataccc ggatgtcgaa
     7801 ctggcgcata tgtacatcga caacgccacc atgcagctga ttaaagatcc atcacagttt
     7861 gacgttctgc tgtgctccaa cctgtttggc gacattctgt ctgacgagtg cgcaatgatc
     7921 actggctcga tgggcatgtt gccttccgcc agcctgaacg agcaaggttt tggactgtat
     7981 gaaccggcgg gcggctcggc accagatatc gcaggcaaaa acatcgccaa cccgattgca
     8041 caaatcctgt cgctggcact gctgctgcgt tacagcctgg atgccgatga tgcggcttcc
     8101 gccattgaac gcgccattaa ccgtgcatta gaagaaggca ttcgcaccgg ggatttagcc
     8161 cgtggcgctg ccgccgttag taccgatgaa atgggcgata tcattgcccg ctatgtggca
     8221 gaaggggtgt aatcatggct aaaacgttat acgaaaaatt attcgacgct cacgtagtgt
     8281 acgaagccga aaacgaaacc ccgctgttat atatcgaccg ccacctggtg catgaagtga
     8341 cctcaccgca ggcgttcgat ggcctgcgcg cccacggtcg cccggtacgt cagccgggca
     8401 aaaccttcgc cactatggat cacaacgtct ctacccagac caaagacatt aatgcctgtg
     8461 gtgaaatggc gcgcatccag atgcaggagc tgattaaaaa ctgcaaagaa tttggcgtcg
     8521 agctgtatga cctgaatcac ccgtatcagg ggatcgtcca cgtaatgggg ccggaacagg
     8581 gcgtcacctt gccggggatg accattgtct gcggcgactc gcataccgcc acccacggcg
     8641 cgtttggcgc actggccttt ggtatcggca cttccgaagt tgaacacgta ctggcaacac
     8701 aaaccctgaa acagggccgt gcgaagacca tgaaaattga agtccagggc aaagccgcgc
     8761 cgggcattac cgcaaaagat atcgtgctgg caattatcgg taaaactggc agcgcaggcg
     8821 gcaccgggca tgtggtggag ttttgcggcg aagcaattcg tgatttaagc atggaaggtc
     8881 gtatgaccct gtgcaatatg gcgatcgaaa tgggcgcaaa agccggtctg gttgctcctg
     8941 acgaaaccac ctttaactat gtcaaaggcc gtctgcatgc gccgaaaggc aaagatttcg
     9001 acgacgccgt tgcctactgg aaaaccctgc aaaccgacga aggcgcaact ttcgataccg
     9061 ttgtcactct gcaagcagaa gaaatttcac cgcaggtcac ctggggcacc aatcccggcc
     9121 aggtgatttc tgtgaacgac aatattcccg atccggcttc gtttgccgat ccggttgaac
     9181 gtgcgtcggc agaaaaagcg ctggcctata tggggctgaa accgggtatt ccgctgaccg
     9241 aagtggctat cgacaaagtg tttatcggtt cctgtaccaa ctcgcgcatt gaagatttac
     9301 gcgcggcagc ggagatcgcc aaagggcgaa aagtcgcgcc aggcgtgcag gcactggtgg
     9361 ttcccggctc tggcccggta aaagcccagg cggaagcgga aggtctggat aaaatattta
     9421 ttgaagccgg ttttgaatgg cgcttgcctg gctgctcaat gtgtctggcg atgaacaacg
     9481 accgtctgaa tccgggcgaa cgttgtgcat ccaccagcaa ccgtaacttt gaaggccgcc
     9541 aggggcgcgg cgggcgcacg catctggtca gcccggcaat ggctgccgct gctgctgtga
     9601 ccggacattt cgccgacatt cgcaacatta aataaggagc acaccatggc agagaaattt
     9661 atcaaacaca caggcctggt ggttccgctg gatgccgcca atgtcgatac cgatgcaatc
     9721 atcccgaaac agtttttgca gaaagtgacc cgtacgggtt ttggcgcgca tctgtttaac
     9781 gactggcgtt ttctcgatga aaaaggccaa cagccaaacc cggacttcgt gctgaacttc
     9841 ccgcagtatc agggcgcttc cattttgctg gcacgagaaa acttcggctg tggctcctcg
     9901 cgtgagcacg cgccctgggc attgaccgac tacggtttta aagtggtgat tgcgccgagt
     9961 tttgctgaca tcttctacgg caatagcttt aacaaccagc tgctgccggt gaaattaagc
    10021 gatgcagaag tggacgaact gtttgcgctg gtgaaagcca atccggggat ccatttcgac
    10081 gtggatctgg aagcgcaaga ggtgaaagcg ggagagaaaa cctatcgctt taccatcgat
    10141 gccttccgcc gccactgcat gatgaatggt ctggacagca tcggcctgac cctgcagcac
    10201 gacgacgcta ttgcgtctta tgaagcaaaa caacctgcgt ttatgaatta gaccccttgc
    10261 ccggtcaaat gaccgggctt tccgctatcg tccacgtcat caaacgtctt taacctttgc
    10321 ggttaaaaat aatgcgacaa cagaaataat cgcaattacc cagtagacag caaagtgccc
    10381 ccaactttgt gcaattgctc cctgaataac accagccaga attaccccgg tagagatagt
    10441 gttggtaaat aaggtggtag ctgcccccgc tcttccaggc attaaatcct gaaaccatag
    10501 catcccaata cccgcaacaa tgccgataaa tacagcgtta aaaagttgca gcgtcatcaa
    10561 cgccatacgg ctatgaaaga aaatcaatcc agtgtaaaac agcactcctg ccaccactgc
    10621 aatgaccatc attcgccgct taccataacg tttgacatag tagccagcca gaatcattgc
    10681 tggtatttcc agtccagctg ccgtccccat caggaaaccc gccagtttgt ctggcaatcc
    10741 taaatcgcta ctgatccaca atggcatatc aataatgtac atagtgttgc aggtccacat
    10801 taacgtcgag gcgacaaata acatccgtac gttactatcc tgccagtcac cttgcattga
    10861 taaagcattt tccgacggca gttctacccg cgccagagca aataactgtg gcattgccgt
    10921 attggccaga gatgccagaa gcacaccaca ggtgataagc gtcagataat gacgattaaa
    10981 tgcaaacagt agcgcattgc caatagccat caaacagcaa aatataatca gttttcgccg
    11041 atcgccctga ctgtcagaac gttttgccaa ccagaggctt acgccgatcc cagcaatagc
    11101 attcaccgta taaaagagtc cgatccagaa aggttgcgcg ccaacctcac gactcagaaa
    11161 taagctcaat gtaggagcct gtagcgcccc ggccaccccc atcataaaag cgaccagcat
    11221 aaatgccgcg taaacaccgt tcatacggcg agccatcgtc attatccaga tcatacgttc
    11281 cctttttagc gcggcgagaa taaaaaaaac cagcaggtat aatctgctgg cgggtgattt
    11341 tacaccaata ctcagtcaca catgatgcag gcaagtcaac tttcagaatt gcggtcatcc
    11401 cactgcatca gtccttcaag catggttaat cgttgtggga cgcttaacca acgcaaccag
    11461 cacaacttcg ctgtcgcggt aaaatagtgc tgataaaact gacgcatggg gcacctcctt
    11521 gcttcatcgt taggaattat tgacttaata tagggaaaat aaaattgctg tcttttgcgc
    11581 aggagttccc cttttatgcc atctgctcgt ctgcaacaac agttcatccg cctgtggcaa
    11641 tgctgcgagg gtaaatcgca ggacacaacg ctcaacgaac tggcagcgtt attgagctgc
    11701 tcgcgtcgtc atatgcgtac cctgctcaac accatgcagg atcgcggctg gctgacgtgg
    11761 gaagcggaag tcgggcgcgg taaacgctcg cgtctgacat tcctctatac cgggctggcg
    11821 cttcagcaac agcgggcgga agacctgctg gagcaggatc gtatcgacca actagtgcag
    11881 ttggttggcg acaaagcgac tgtgcggcaa atgctggttt ctcatctggg ccgcagcttc
    11941 cgccaggggc ggcacatcct gcgcgtgctc tactatcgtc cgttgcgtaa tctgctacct
    12001 ggcagcgcat tgcgccgttc cgaaacccat attgcccggc aaatcttcag ttcgctaacg
    12061 cgcataaatg aggaaaatgg ggaactggaa gcagacatcg cccaccactg gcagcaaata
    12121 tcaccgcttc actggcgttt ctttttgcgt ccaggagtcc attttcatca tggtcgtgaa
    12181 ctggaaatgg acgacgtgat cgcctcttta aaacgaatca atacgctgcc gctctattcg
    12241 catattacgg acattgtgtc gccgacgccc tggacgctgg atatccatct cacgcagccg
    12301 gaccgctggt tgccgttact gctggggcaa gttccggcga tgatcctgcc gtgcgaatgg
    12361 gaaaccctca gtaactttgc cagccatcct attggcaccg gtccgtatgc ggtgattcgc
    12421 aacagcacca atcaactgaa aattcaggca ttcgatgact tcttcggtta ccgggcatta
    12481 atcgacgaag ttaacgtctg ggttctgccg gaaattgccg acgagccagc cggtggtctg
    12541 atgctaaaag gtccacaggg cgagaaaaaa gagattgaaa gccgcctgga ggaaggttgc
    12601 tactatttac tgttcgacag ccgcacccat cgcggggcga atcagcaagt cagggactgg
    12661 gtaagctatg tgctttctcc aactaatctg gtctatttcg ctgaggaaca gtaccagcaa
    12721 ctgtggttcc cggcttatgg actgctcccc cgttggcacc atgcccgcac cataaagagc
    12781 gaaaaaccgg ctggcctgga aagcctcacc ctgacctttt atcaggatca cagtgagcat
    12841 cgggtgattg ccgggatcat gcagcagatt ctggcaagtc accaggtcac gctggaaatc
    12901 aaagagatca gctacgatca gtggcatgaa ggagagatcg agagcgatat ctggcttaat
    12961 agcgccaact ttacgctgcc gctggatttt tcgctgttcg cgcacttgtg cgaggtgccg
    13021 ctgctccaac actgtattcc gatcgactgg caagccgacg ccgcccgctg gcgcaatggc
    13081 gaaatgaacc tggcgaactg gtgccagcaa ctggtcgcca gtaaagcaat ggtgccgctt
    13141 atccaccact ggctgatcat tcagggacaa cgcagtatgc gcggcctgcg tatgaatacc
    13201 ctcggctggt tcgattttaa atcagcgtgg tttgcgccac cggatccatg attgctggta
    13261 gataacaaaa tcactacact aacgccgttc tcaacggggt gccacgcaaa cgcgtgcgct
    13321 gagaaaatac ccgtcgaacc tgatccggat aacgccggcg aagggatttg aggctccttc
    13381 tcaagtcctt tgccactctt ttttgaggtg caaagtgtta aaaaaatgtc ttcccctgct
    13441 gttgctgtgc acagcgcccg ttttcgctaa acccgttctg actgtttata cctacgattc
    13501 cttcgccgcc gactgggggc ctggtccaaa gatcaaaaaa gcttttgaag ccgactgtaa
    13561 ctgcgaactg aaactggtgg cgctggaaga tggcgtttcg cttctcaacc gtctacggat
    13621 ggaaggcaaa aatagtaaag ccgatgtggt gctggggctg gataacaatc tgttagacgc
    13681 cgccagtaaa accggactat ttgccaaaag cggtgtggca gcggatgccg ttaacgttcc
    13741 cggcggctgg aataatgaca ctttcgtccc gtttgattac ggctatttcg ccttcgtcta
    13801 tgacaagaac aaactgaaaa acccgccgca aagcctgaaa gaattggttg agagcgatca
    13861 gaactggcgg gtgatttatc aggatccgcg taccagcacg ccggggctgg gtctgctgtt
    13921 gtggatgcaa aaagtctatg gcgatgacgc cccacaagcc tggcaaaaac tggcgaagaa
    13981 aaccgtcacg gtcaccaaag gctggagcga agcctacggc ctgtttttaa aaggtgaaag
    14041 cgatctggta ctgagttaca ccacctctcc ggcttatcac attctcgaag agaaaaaaga
    14101 taactacgcc gccgcgaact ttagcgaagg tcactatctg caggtggaag tcgccgcccg
    14161 caccgctgcc agcaagcagc cggagctggc gcaaaaattc ctccagttta tggtttctcc
    14221 ggcttttcag aatgcgatcc caaccggtaa ctggatgtat ccggtggcaa atgtcacgct
    14281 gcctgccggg tttgaacaat tgaccaaacc ggccaccacg ctggagttca caccagctga
    14341 agtggcggca caacgtcagg catggattag cgaatggcaa cgcgccgtca gccgttaatt
    14401 cccggctggt taattccagg tgtaagcgcc gccacgctgg tggtagcggt tgcgctcgcg
    14461 gcgtttctcg ccctgtggtg gaacgcgccg cagggtaact gggtggcagt ctggcaggac
    14521 agctacctgt ggcatgtggt gcgcttctcc ttctggcagg cgtttctctc ggcgctactc
    14581 tctgtcgtac ccgcgatatt cctcgcccgc gccctctatc gcaggcgctt tccgggtcgg
    14641 ctggcgctgt tgcgtctgtg cgcaatgacc ttgatcctcc cggtgctagt tgctgttttc
    14701 ggcattctta gcgtctatgg tcgtcagggc tggctggcat cgctctgcca atcgctcggt
    14761 ctggagtgga ccttttcgcc ctacggcctg caaggcattt tgctggcgca cgtatttttt
    14821 aatctgccga tggcgagccg cttattactc caggcactgg aaaacattcc cggcgaacaa
    14881 cgtcagcttg ccgcccagct tgggatgcgc ggctggcatt ttttccgctt cgtcgaatgg
    14941 ccgtggctac ggcgacaaat cccgccggtt gccgcgctta tctttatgct ctgtttcgcc
    15001 agcttcgcca ccgtgctatc gctgggcggc ggtccgcagg caaccactat cgagctggct
    15061 atttatcagg cgctgagtta cgactacgat cctgcccgcg cggcgatgct ggcgctgctc
    15121 cagatggtgt gttgccttgg gctggtgctg ttgagtcagc gactaagtaa ggccattgcg
    15181 cccggcacca cgctgctgca aggctggcgc gatccggacg atcgtctgca tagccgcatt
    15241 tgcgacacgg tgttaatagt gctggcgctg ctgctgttgc tgccaccgtt gctggcggtg
    15301 atcgtcgatg gggtaaatcg ccagttgccg gaagtgctgg cacaaccggt gctgtggcag
    15361 gcgctgtgga cctcgttgcg tattgcgctg gcggcaggtg tattgtgcgt agtgctgacc
    15421 atgatgctgc tatggagcag tcgcgaactg cgggcgcggc agaaaatgct ggcgggtcag
    15481 gcgctggaga tgagcggcat gttgatcctc gccatgccgg ggattgtgct ggctaccggc
    15541 ttctttttac tgctcaacaa cactatcggc ctgccacaat ctgctgacgg cattgtgatt
    15601 ttcaccaatg cgttaatggc gatcccttat gcgctgaaag tactggaaaa cccgatgcgc
    15661 gatatcaccg cccgctacag tatgttgtgt cagtcgctgg gcattgaagg ctggtcgcgc
    15721 ttaaaagtgg tcgagctgcg cgccctgaaa cgtccactgg cgcaggcgct ggcttttgcc
    15781 tgcgtgctgt cgattggtga ttttggcgta gtggcgttgt tcggtaacga tgatttccgt
    15841 accctgccgt tttatctcta ccagcaaatt ggctcctatc gcagccagga cggcgcggtc
    15901 accgcgttaa ttctgctact actctgtttt ctgctgttta ccgtgattga aaaactaccg
    15961 gggcgaaatg ttaaaactga ctgatatcac ctggctttat caccatttgc cgatgcgttt
    16021 tagcttaacg gtagaacgcg gcgagcaggt ggcaatcctc gggccaagcg gcgcgggtaa
    16081 aagtaccctg ctgaatttga ttgccggttt tctgacgcca gccagcggtt cgctgactat
    16141 cgatggtgtt gatcacacca cgacgccgcc gtcacgccgt ccggtgtcga tgctgtttca
    16201 ggagaacaac ctgttcagcc atctgacggt cgcgcagaac atcgggctgg ggctaaatcc
    16261 ggggttgaaa ctgaacgcgg cacagcagga gaaaatgcac gcgattgccc gtcagatggg
    16321 gattgataat ttaatggcgc ggttaccggg cgagctttcc ggcggtcagc gacagcgcgt
    16381 ggcgttagcg cgttgtctgg tgcgcgaaca gccgatttta ttgctcgatg aaccgttctc
    16441 tgcgctcgat ccggcgttac gtcaggagat gctgacgctg gtgagcacga gctgccagca
    16501 gcaaaaaatg acgctattga tggtgtcgca cagtgtggaa gatgcggcgc ggatcgccac
    16561 acgctcggtg gtggtcgccg acgggcgcat cgcctggcag ggtaagactg aagaattgtt
    16621 gagcggtaag gcgagtgctt cggcaatatt ggggattacg ggttagtatg cactgtcaga
    16681 tgcggcgtga acaccttatc cgacctacgg acagtgcgtt ttgtaggcct gataagacgc
    16741 ggtcagcgtc gcatgaggca acccgcacaa gacaatcccc taaaccccaa ccactttacg
    16801 caaaatatcg atatacaccg gcatcaacgg gtggcgaatt aacaccacca gcgccaccac
    16861 gccgatggca gaaatcaacg gcgtcagcca caacaaacgg ccgcgcgaca aataatgact
    16921 taaacgatcg gttgctttac cgctgcgcca taaccgccag cacagccagc caccaaccca
    16981 caaaaatacc gccgtcgcca gcagcaacca tttaaactcg ccgctctgca ttccggctgg
    17041 aatatcgatc gccgcgcccg ccagaatccc aggcaggaag taaaacggtg gccacagcag
    17101 gcagccgata atattcggcg taatgaattt tgccaccggc agatccagca ttcccgccac
    17161 cattggcacc agcggacgcg tcgggccaac aaagcgaccg accagaatgg tgaacatgct
    17221 gtgttgatgc aacgcatgtt cagttttatc gagtaatgct ttgtttttct tcagaaatga
    17281 ccagcgatgc aacggctttt taaaacgcca gcccagccag aaggagatcc agtcgcccag
    17341 cagacaaccg acaatccctg ccagccaagc gtgccagaaa cttaactcgc cgctgccaat
    17401 cagcgctccc agccccgcca tcagcaccgt accgggtaga atcaaaccga ccagcgccag
    17461 cgactccaga aaggccacca acaccaccgc catcaatgaa tacacggtag attgggtaat
    17521 aaagtgttcc agcaatgctt gcataatgtg cctgtcaaat ggacgaagca gggattctgc
    17581 aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat tatgacaact
    17641 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
    17701 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
    17761 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
    17821 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
    17881 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
    17941 ggtgatcgct gatgtactga caagcctcgc gtacacgatt atccatcggt ggatggagcg
    18001 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
    18061 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
    18121 aatacggctg gtgcgcttca tccgggcgaa agaaacccgt attggcaaat attgacggcc
    18181 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
    18241 cgcgagcctc cggatgacaa ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
    18301 cacccggtcg gcagacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
    18361 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
    18421 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
    18481 gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa
    18541 ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc
    18601 taacccaacc ggtaaccccg cttattaaaa gcattctgta acaaagcagg accaaagcca
    18661 tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt
    18721 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatccta
    18781 cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt tggatggagt
    18841 gaaacgatgg cgattgcaat tggcctcgat tttggcagtg attctgtgcg agctttggcg
    18901 gtggactgcg ccagcggtga agagatcgcc accagcgtag agtggtatcc ccgttggcaa
    18961 aaagggcaat tttgtgatgc cccgaataac cagttccgtc atcatccgcg tgactacatt
    19021 gagtcaatgg aagcggcact gaaaaccgtg cttgcagagc ttagcgtcga acagcgcgca
    19081 gctgtggtcg ggattggcgt tgacagtacc ggctcgacgc ccgcaccgat tgatgccgac
    19141 ggtaacgtgc tggcgctgcg cccggagttt gccgaaaacc cgaacgcgat gttcgtattg
    19201 tggaaagacc acactgcggt tgaagaagcg gaagagatta cccgtttgtg ccacgcgccg
    19261 ggcaacgttg actactcccg ctatattggc ggtatttatt ccagcgaatg gttctgggca
    19321 aaaatcctgc atgtgactcg ccaagacagc gccgtggcgc aatctgccgc atcgtggatt
    19381 gagctgtgcg actgggtgcc agctctgctt tccggtacca cccgcccgca ggatattcgt
    19441 cgcggacgtt gcagcgccgg gcataaatct ctgtggcacg aaagctgggg cggcttgccg
    19501 ccagccagtt tctttgatga gctggacccg atcctcaatc gccatttgcc ttccccgctg
    19561 ttcactgaca cctggactgc cgatattccg gtgggcacct tatgcccgga atgggcgcag
    19621 cgtctcggcc tgcctgaaag cgtggtgatt tccggcggcg cgtttgactg ccatatgggc
    19681 gcagttggcg caggcgcaca gcctaacgca ctggtaaaag ttatcggtac ttccacctgc
    19741 gacattctga ttgccgacaa acagagcgtt ggcgagcggg cagttaaagg tatttgcggt
    19801 caggttgatg gcagcatggt gcctggattt atcggtctgg aagcaggcca atcggcgttt
    19861 ggtgatatct acgcctggtt tggtcgcgta ctcggctggc cgctggaaca gcttgccgcc
    19921 cagcatccgg aactgaaagc gcaaatcaac gccagccaga aacaactgct tccggcgctg
    19981 accgaagcat gggccaaaaa tccgtctctg gatcacctgc cggtggtgct cgactggttt
    20041 aacggtcgtc gcacgccaaa cgctaaccaa cgcctgaaag gggtgattac cgatcttaac
    20101 ctcgctaccg acactccgct gctgttcggc ggtttgattg ctgccaccgc ctttggcgca
    20161 cgcgcaatca tggagtgctt taccgatcag gggatcgccg tcaataacgt gatggcgctg
    20221 ggcggcatcg cgcggaaaaa ccaggtcatt atgcaggcct gctgcgacgt gctgaatcgc
    20281 ccgctgcaaa ttgttgcctc tgaccagtgc tgtgcgctcg gtgcggcgat ttttgctgcc
    20341 gtcgccgcga aagtgcacgc agacatccca tcagcccagc aaaaaatggc cagtgcggta
    20401 gagaaaaccc tgcaaccgcg cagcgaacag gcacaacgct ttgaacagct ttatcgccgc
    20461 tatcagcaat gggcgatgag cgccgaacaa cactatcttc caacttccgc cccggcacag
    20521 gctgcccagg ccgttgcgac tctataagga cacgataatg gcgatttttg ataattatga
    20581 agtgtggttt gtcattggca gccagcatct gtatggcccg gaaaccctgc gtcaggtcac
    20641 ccaacatgcc gagcacgttg ttaatgcgct gaatacggaa gcgaaactgc cctacaaact
    20701 ggtgttgaaa ccgctgggca ccacgccgga tgaaatcacc gctatttgcc gcgacgcgaa
    20761 ttacgacgat cgttgcgctg gtctggtggt gtggctgcac accttctccc cggccaaaat
    20821 gtggatcaac ggcctgacca tgctcaacaa accgttgctg caattccaca cccagttcaa
    20881 cgcggcgctg ccgtgggaca gtatcgatat ggactttatg aacctgaacc agactgcaca
    20941 tggcggtcgc gagttcggct tcattggcgc gcgtatgcgt cagcaacatg ccgtcgttac
    21001 cggtcactgg caggataaac aagcccatga gcgtatcggc tcctggatgc gtcaggcggt
    21061 ctctaaacag gatacccgtc atctgaaagt ctgccgattt ggcgataaca tgcgtgaagt
    21121 ggcggtcacc gatggcgata aagttgccgc acagatcaag ttcggtttct ccgtcaatac
    21181 ctgggcggtt ggcgatctgg tgcaggtggt gaactccatc agcgacggcg atgttaacgc
    21241 gctggtcgat gagtacgaaa gctgctacac catgacgcct gccacacaaa tccacggcga
    21301 aaaacgacag aacgtgctgg aagcggcgcg tattgagctg gggatgaagc gtttcctgga
    21361 acaaggtggc ttccacgcgt tcaccaccac ctttgaagat ttgcacggtc tgaaacagct
    21421 tccaggtctg cccgtacagc gtctgatgca gcagggttac ggctttgcgg gcgaaggcga
    21481 ctggaaaacc gccgccctgc ttcgcatcat gaaggtgatg tcaaccggtc tgcagggcgg
    21541 cacctccttt atggaggact acacctatca cttcgagaaa ggtaatgacc tggtgctcgg
    21601 ctcccatatg ctggaagtct gcccgtcgat tgccgtagaa gagaaaccga tcctcgacgt
    21661 tcagcatctc ggtattggtg gtaaggacga tcctgcccga ctgatcttca atacccaaac
    21721 cggtccagcg attgtcgcca gcctgattga tctcggcgat cgttaccgtc tgctggttaa
    21781 ctgtatcgac acggtgaaaa caccgcactc cctgccgaaa ctgccggtgg cgaatgcact
    21841 gtggaaagcg caaccggatc tgccaactgc ttccgaagcg tggatcctcg ctggtggcgc
    21901 gcaccatacc gtcttcagcc atgcgctgaa cctcaacgat atgcgccagt tcgccgagat
    21961 gcacgacatt gaaatcacgg tgattgataa cgacacccgc ctgccagcgt ttaaagacgc
    22021 actgcgctgg aacgaagtgt attacggatt tcgtcgctaa gtagccacat caggtatgta
    22081 acgcctgatg cgacgctgac gcgtcttatc tggcctacac gctgcgattt tgtaggccgg
    22141 ataagcaaag cgcatccggc attcaacgcc tgatgcgacg ctggcgcgtc ttatcaggcc
    22201 tacgcgctgc gattttgtag accggataag cgaagcgcat ccggcacgaa ggagtcaaca
    22261 tgttaaaaga tctcaaacgc caggtattag aagccaacct ggcgctgcca aaacacaacc
    22321 tggtcacgct cacatggggc aacgtcagcg ccgttgatcg cgagcgcggc gtctttgtga
    22381 tcaaaccttc cggcgtcgat tacagcgtca tgaccgctga cgatatggtc gtggttagca
    22441 tcgaaaccgg tgaagtggtt gaaggtacga aaaagccctc ctccgacacg ccaactcacc
    22501 ggctgctcta tcaggcattc ccctccattg gcggcattgt gcatacgcac tcgcgccacg
    22561 ccaccatctg ggcgcaggcg ggtcagtcga ttccagcaac cggcaccacc cacgccgact
    22621 atttctacgg caccattccc tgcacccgca aaatgaccga cgcagaaatc aacggcgaat
    22681 atgagtggga aaccggtaac gtcatcgtag aaacctttga aaaacagggt atcgatgcag
    22741 cgcaaatgcc cggcgttctg gtccattccc acggcccgtt tgcatggggc aaaaatgccg
    22801 aagatgcggt gcataacgcc atcgtgctgg aagaggtcgc ttatatgggg atattctgcc
    22861 gtcagttagc gccgcagtta ccggatatgc agcaaacgct gctggataaa cactatctgc
    22921 gtaagcatgg cgcgaaggca tattacgggc agtaatgact gtataaaacc acagccaatc
    22981 aaacgaaacc aggctatact caagcctggt tttttgatgg attttcagcg tggcgcaggc
    23041 aggttttatc ttaacccgac actggcggga caccccgcaa gggacagaag tctccttctg
    23101 gctggcgacg gacaacgggc cgttgcaggt tacgcttgca ccgcaagagt ccgtggcgtt
    23161 tattcacgcc gatcaggttc cccgcgctca gcatattttg cagggtgaac aaggctttcg
    23221 tctgacaccg ctggcgttaa aggattttca ccgccagccg gtgtatggcc tttactgtcg
    23281 cgcccatcgc caattgatga attacgaaaa gcgtctgcgt gaaggtggcg ttaccgtcta
    23341 cgaggccgat gtgcgcccgc cagaacgcta tctgatggag cggtttatca cctcaccggt
    23401 gtgggtcgag ggtgatatgc acaatggcac tatcgttaat gcccgtctga aaccgcatcc
    23461 cgactatcgt ccgccgctca agtgggtttc tatagatatt gaaaccaccc gccacggtga
    23521 gctgtactgc atcggcctgg aaggctgcgg gcagcgcatc gtttatatgc tggggccgga
    23581 gaatggcgac gcctcctcgc ttgatttcga actggaatac gtcgccagcc gcccgctgct
    23641 gctggaaaaa ctcaacgcct ggtttgcaaa ccacgatccc gatgtgatca tcggctggaa
    23701 cgtggtgcag ttcgatctgc gaatgctgca aaaacatgcc gagcgttacc gtcttccgct
    23761 gcgtcttggg cgcgataata gcgagctgga gtggcgcgag cacggcttta aaaacggcgt
    23821 cttttttgcc caggctaaag gtcggctaat tatcgacggt atcgaggcgc tgaaatccgc
    23881 gttctggaat ttctcttcat tctcgctgga aactgtcgct caggagctat taggcgaagg
    23941 aaaatctatc gataacccgt gggatcgaat ggacgaaatt gaccgccgtt tcgccgaaga
    24001 taaacctgcg ctggcaactt ataacctgaa agattgcgag ctggtgacgc agatcttcca
    24061 caaaactgaa atcatgccat ttttactcga acgagcaacg gtgaacggcc tgccggtgga
    24121 tcggcacggc ggttcggtgg cagcgtttgg tcatctctat tttccgcgaa tgcatcgcgc
    24181 tggttatgtc gcgcctaatc tcggcgaagt gccgccgcac gccagccctg gcggctacgt
    24241 gatggattca cggccagggc tttatgattc agtgctggtg ctggactata aaagcctgta
    24301 cccgtcgatc atccgcacct ttctgattga tcccgtcggg ctggttgaag gcatggcgca
    24361 acctgatcca gagcacagta ccgaaggttt tctcgatgcc tggttctcac gggagaaaca
    24421 ttgcctgccg gagattgtga ctaacatctg gcacgggcgc gatgaagcca aacgtcaggg
    24481 taataagccg ctttcgcagg cgctgaagat cattatgaat gccttttatg gcgtgctcgg
    24541 caccaccgcc tgccgcttct tcgatccgcg cctggcatcg tcgatcacca tgcgtggtca
    24601 tcagatcatg cggcagacca aagcattgat tgaagcgcag ggctacgaca ttatctacgg
    24661 agataccgac tcgacttttg tctggctaaa aggtgcacat tcggaagaag aggcggcgaa
    24721 aatcggtcgt gcactggtgc agcacgttaa cgcctggtgg gcggaaacgc tgcaaaaaca
    24781 acggctgacc agcgcattag aactggagta tgaaactcat ttctgccgtt ttcttatgcc
    24841 aaccattcgc ggagctgata ccggcagtaa aaagcgctac gccgggctga ttcaggaggg
    24901 cgacaagcag cggatggtgt ttaaagggct ggaaaccgtg cgcaccgact ggacgccgct
    24961 ggcccagcag tttcagcagg agctgtacct gcgtatcttc cgcaacgagc catatcagga
    25021 gtacgtccgc caaaccatcg acaaactgat ggcgggtgaa ctggatgcgc ggctggttta
    25081 ccgtaaacgc cttcgccgtc cgctgagcga atatcagcgt aatgttccgc ctcatgtacg
    25141 cgccgctcgc cttgccgatg aagaaaatca aaagcgcggt cgccccttgc aatatcagaa
    25201 tcgcggcacc attaagtacg tatggaccac caacggcccg gagccgctgg actaccaacg
    25261 ttcaccactg gattacgaac actatctgac ccgccagcta caacccgtgg cggagggaat
    25321 actccctttt attgaggata attttgctac acttatgacc gggcaacttg ggctattttg
    25381 agcaaaaaaa agagttcgcc agataccatt ttgatgcgtg acgaatgctt tgccatccag
    25441 taccatagcg ccctttccat tcctggacct gaataacacc actacctcat aagcacggta
    25501 gcgggtggtt attgcctgca attaaagata tagagccgaa cacatatgcc ttttacactt
    25561 ggtcaacgct ggatcagcga tacagaaagc gaattgggac ttggaaccgt tgtcgcggtg
    25621 gatgcgcgaa ctgtcacttt acttttccca tctactggtg aaaaccgtct gtacgcacgc
    25681 agtgattccc ccgtgacccg cgtgatgttc aaccctggtg ataccattac cagccatgac
    25741 ggctggcaga tgcaagtcga agaagtaaaa gaagaaaatg gcttgctgac ctatatcggt
    25801 actcgcctgg atactgaaga gtccggcgta gccctgcgtg aagttttcct tgatagcaaa
    25861 ctggtgttca gcaaaccgca ggaccgtctg tttgccgggc agattgaccg tatggaccgc
    25921 tttgcgctgc gttatcgcgc gcgtaagtat tccagcgaac agttccgtat gccgtacagc
    25981 ggcctgcgcg gtcagcgtac cagcctgatc ccgcatcagc tcaacatcgc tcatgatgtt
    26041 ggtcgccgcc acgcgccgcg cgtcctgctg gctgacgaag tgggtttagg gaaaaccatt
    26101 gaagccggga tgatcctgca tcagcaactg ctctctggcg ctgctgaacg tgtgctgatt
    26161 atcgtcccgg aaaccttaca gcatcagtgg ctggtagaaa tgctgcgccg tttcaacctg
    26221 cgctttgctc tgtttgatga tgagcgttat gccgaagctc agcacgatgc ttacaacccg
    26281 tttgacaccg aacagctggt gatttgctcg ctggattttg cccgtcgtag caaacagcgc
    26341 ttggaacatc tctgtgaagc cgaatgggac ctgctggtgg tcgatgaagc gcatcacctg
    26401 gtgtggagcg aagatgcgcc aagccgtgaa tatcaggcca ttgaacaact ggcagagcac
    26461 gtgccgggcg ttctgctgct gaccgcgacc ccggaacagc tggggatgga aagccacttc
    26521 gcccgtctgc gtctgctgga cccgaaccgt ttccacgatt ttgcgcagtt cgttgaagag
    26581 cagaaaaatt atcgtccggt tgcggacgcc gttgccatgc tgctggcagg taacaaactg
    26641 agcaatgacg aactgaacat gctcggcgag atgatcggcg agcaggatat cgagccgctg
    26701 ttgcaggcag caaacagcga cagcgaagat gcccagagcg cccgtcagga gctggtttcg
    26761 atgctgatgg atcgccacgg caccagccgc gtgctgttcc gtaacacgcg taacggtgtg
    26821 aaaggattcc cgaaacgcga gctgcacacc attaagctgc cgctaccgac gcagtatcag
    26881 acggctatta aagtctccgg cattatgggc gcacgtaaaa gtgcggaaga tcgtgctcgc
    26941 gatatgctct acccggagcg tatttatcag gaatttgaag gtgataacgc cacctggtgg
    27001 aacttcgatc cgcgcgttga gtggctgatg ggctacctga ccagccatcg ctctcagaaa
    27061 gtgctggtga tctgcgccaa agctgccact gcgctgcaac tggagcaggt actgcgcgaa
    27121 cgtgaaggta ttcgcgctgc ggtgttccac gaaggtatgt cgattatcga acgtgaccgc
    27181 gctgccgcct ggtttgccga agaagacacc ggcgcacagg tactgctgtg ctcagaaatc
    27241 ggttctgaag gacgtaactt tcagttcgcc agccacatgg tgatgtttga cctgccattc
    27301 aacccggatc tactggagca gcgtattggt cgtctggatc gtatcggcca ggcgcacgat
    27361 attcagatcc atgtgcctta tctggagaaa accgctcagt cggtgctggt gcgctggtat
    27421 cacgaaggtc tggatgcatt tgagcacacc tgcccgaccg gacgcactat ttacgatagc
    27481 gtatacaacg atctgattaa ctatctggct tcaccggatc aaaccgaagg ctttgacgat
    27541 ctgatcaaaa gctgccgcga gcaacatgaa gcgctgaaag cacagctgga acagggtcgt
    27601 gaccgcctgc tggaaatcca ctccaacggt ggcgaaaaag cccaggaact ggcagaaagc
    27661 attgaagagc aggatgacga taccaacctg atcgccttcg ccatgaacct gttcgatatt
    27721 atcggtatca atcaggacga tcgcggcgac aacatgatcg tgctgacgcc gtccgatcat
    27781 atgctggtgc cggacttccc tggcctgtcg gaagatggca tcaccatcac ctttgatcgt
    27841 gaagtggcgc tggcgcgtga agatgcacag tttattacct gggagcatcc gctgatccgc
    27901 aacggtctgg atctgatcct ttctggcgat accggtagca gcacgatttc actgttaaaa
    27961 aacaaagcgt tgccggtagg tacgctgttg gtggaactga tttatgtggt tgaagcccag
    28021 gctccgaagc agttgcagct caaccgcttc ctgccaccga cgccggtacg tatgctgctg
    28081 gataaaaacg gcaacaacct ggcggcgcag gtagagtttg aaacctttaa ccgccagctt
    28141 aacgcggtta accgtcacac cggcagcaaa ctggttaacg ccgtgcagca ggatgttcac
    28201 gctatccttc aactgggtga agcgcagatc gagaaatctg cccgtgcatt gattgatgca
    28261 gcgcgtaacg aagccgacga aaaactgtct gccgagctgt ctcgtctgga agctctgcgt
    28321 gcagtgaacc cgaacattcg tgacgacgaa ctgaccgcca ttgagagcaa ccgtcagcag
    28381 gtaatggaaa gcctggatca ggcaggttgg cgtctggatg ccctgcgttt gatcgttgta
    28441 acgcatcagt aacggagccg aaaatgggga tggaaaacta caatccaccg caggaaccct
    28501 ggttggttat cctgtatcag gatgaccata ttatggtggt caacaagccg agcggtttgt
    28561 tgtcagtgcc gggtcgtctg gaagagcaca aagacagcgt gatgacgcgc attcagcgtg
    28621 attatccgca ggcagaatcg gtgcatcgtc tggatatggc taccagcggc gtgattgtag
    28681 tggcgctgac caaagccgcg gagcgggagt taaaacgcca gttccgcgag cgcgagccga
    28741 aaaagcagta tgtggcccgc gtctgggggc atccatcccc cgcagaaggt ctggtggatc
    28801 tgccgctgat ttgcgactgg ccaaaccgcc cgaaacagaa agtctgttac gaaacgggta
    28861 aacctgcgca gacggaatat gaagtggtgg agtatgcggc ggataacacg gcaagagtgg
    28921 tgttaaaacc gattaccggg cgttcgcatc aattgcgtgt gcatatgctg gcgctggggc
    28981 atccgattct cggcgatcgt ttttatgcat caccagaagc gagagcgatg gcaccgcgtt
    29041 tgttgctaca tgcagagatg ttgacgatta cccatccggc gtatggcaat agtatgacgt
    29101 ttaaagcgcc agcggatttt taatcgtcac gctggcgcag ttcggtaggc ggataaggcg
    29161 ttcacgccgc atccggcaat tgtgcgccga ttgcctgatg cgacgcgggc cgcgtcttat
    29221 caggcctaca gtttaaaact tatttaaacc ctttctgctg ctttatcagc tcatatgcct
    29281 gctgaatttc ctgcgctttc tgcttcgcca tctccatcat ctcaggcggc aaacctttcg
    29341 ccaccagctt atcgggatgg tgttcactca tcagcttacg gtaggcacgt ttgatggtgg
    29401 tcgcatcatc cgtcggcttc acgcccagca cattacaggc atcttccagc gtcgggccac
    29461 gctgcgcttg ctgccagtta ccaccgccag attgctgctg ataaccgccg ccaaactgtg
    29521 caccgccctg catcatgcgc aaaaactggt caaactgggc gcgggagatc cctaattctt
    29581 cagcaatgac atacagcacc gcccgttcat tcgggtgcag tgaaccatca gcaaacgccg
    29641 cctgaatctg gatctccaga aacatacgaa ttaagtcaaa acgaccaaag cagacactgc
    29701 gaaactggcg catcttttcg cgcagcgggt aattatctga ttttcccacc cggaacgcgt
    29761 tttgcgccgc agtacgggaa gcgccatgaa ggttcattcg gtccatcaac tggctggcga
    29821 tatgaatatc agcctccgtg acgcgacctt tggatttggt taaatgcccc atcacttcaa
    29881 aagtggtggc aaaaaacagc gcctgacgct cacgctggtt ggcgaaccac gccattttac
    29941 ggctacgggc tttatcaaac atatggccaa ttaacaggcc taacactacg ccccaaaagc
    30001 cgccgcccat cagtaaggcc acggccacgc caatgatttt tccccaatac tgcatatatt
    30061 ccccaaatcg acacacggat atcagggcta tctcccacaa tataaaggtg cttttaccgt
    30121 tttccggctg cggtcaattg tgggacatcg cctataattt gcattatcat acctgtcatt
    30181 caatgccgtg cctaacacca ctgacgctat tcggacagga ttaacactag cgtagagatg
    30241 acgagtacgt tagtctctga ccgtttgtca cgcgcaacgt taccgatgat ggaacaataa
    30301 aatcaacgta tgaaaaaacg tatccccact ctcctggcca ccatgattgc caccgccctt
    30361 tatagtcaac agggactggc agccgacctc gcctcacagt gcatgttggg cgtgccaagc
    30421 tatgaccgtc ctctggtaca gggcgatacc aatgacttac ccgtgactat caatgctgac
    30481 cacgcgaaag gggactaccc ggatgacgcc gtgtttactg gcagcgtgga tatcatgcag
    30541 ggtaacagcc gtctgcaggc cgacgaagtg cagctccatc aaaaagaggc accaggacaa
    30601 ccggagccgg tacgtaccgt tgatgcgctc ggtaatgtcc attacgacga taaccaggtg
    30661 atcctcaaag ggccgaaagg ctgggcgaat ctgaacacca aagataccaa cgtctgggaa
    30721 ggtgattacc agatggtggg tcgccagggt cgcggtaaag cggacctgat gaaacaacgt
    30781 ggcgaaaacc gctataccat tctggataac ggtagcttta cctcctgtct gccgggttct
    30841 gacacctgga gcgtggtagg tagcgaaatt attcatgacc gcgaagaaca agttgcggag
    30901 atctggaacg cccgctttaa ggtgggtccg gtaccgatct tttatagccc ctatttgcag
    30961 ttgccggtgg gtgacaaacg tcgctctggt ttcttgatcc cgaacgccaa gtacaccacc
    31021 accaactact ttgagttcta cctgccatat tactggaaca tcgcgccaaa tatggatgcc
    31081 accatcacgc cgcattatat gcatcgtcgt ggcaacatca tgtgggagaa cgaattccgc
    31141 tacctctccc aggcgggcgc tggcttgatg gaactggact atctgccttc agataaagtc
    31201 tatgaagatg aacacccgaa cgatgacagt tcacgtcgtt ggttattcta ctggaaccac
    31261 tccggggtca tggatcaggt gtggcgtttc aacgtcgact acaccaaggt cagcgatcct
    31321 agctacttca atgatttcga taacaagtac ggttccagta ctgacggcta cgcaacgcaa
    31381 aaattcagcg ttggctatgc ggtgcaaaac ttcaatgcca ccgtttcaac caagcagttc
    31441 caggttttca gcgaacagaa caccagtagc tactcggcag agccgcagtt agacgttaat
    31501 tactaccaga atgatgttgg tccgtttgat acgcgtattt acggccaggc agtgcacttt
    31561 gttaacacca gagacgacat gcctgaagca acccgtgttc acctggaacc gaccatcaat
    31621 ttgccgctct ctaataactg gggcagcatc aataccgaag cgaagtttct ggcaacccat
    31681 tatcagcaaa ccaatcttga ctggtataac tccagaaaca cgaccaagct ggacgaatcc
    31741 gttaaccgcg taatgccgca attcaaagtt gacggcaaaa tggtctttga acgcgatatg
    31801 gaaatgctgg ctccgggtta tacccaaacg ctggaaccgc gcgcgcagta tttgtacgtg
    31861 ccgtatcgcg atcagagcga catctataac tacgactcgt ctctgctgca atctgactac
    31921 tctggcctgt tccgggaccg gacttacggc ggtcttgacc gtattgcctc cgctaaccag
    31981 gtgacgaccg gtgtcacatc tcgcatatat gatgatgctg ccgttgaacg ttttaatatt
    32041 tccgttggtc aaatctacta tttcacggag tctcgcactg gcgatgacaa cataacatgg
    32101 gagaatgacg acaaaacggg ctcactggtg tgggcaggcg acacctactg gcgtatctcc
    32161 gagcgttggg gattgcgtgg cgggattcag tacgatacac gtctggataa cgtagcgacc
    32221 agtaactcca gcattgaata ccgtcgggat gaagaccgtc tggtacagct gaattaccat
    32281 tacgccagcc cggaatatat tcaggctacg ctgcctaagt actattccac tgctgagcaa
    32341 tataagaatg gtatttcgca ggtaggtgct gtcgccagca ggccaattgc cgatcgttgg
    32401 tccattgttg gggcctacta ctacgacacc aatgcgaaca agcaagccga ctctatgtta
    32461 ggtgtgcaat acagctcctg ctgctatgca attcgcgtcg gttacgagcg gaagctgaac
    32521 ggttgggata acgataaaca acatgcggta tatgacaacg caatcggctt taacatcgaa
    32581 cttcgcggcc tgagctccaa ctacggtctg ggtacgcaag agatgctgcg ttcgaacatt
    32641 ctgccgtatc aaaacacttt gtgatttgtt gatttaccac gtaatccgca gtgcggttaa
    32701 ttgaaatgga aaaagtatga agaactggaa aacgctgctt ctcggtatcg ccatgatcgc
    32761 gaataccagt ttcgctgccc cccaggtagt cgataaagtc gcagccgtcg tcaataacgg
    32821 cgtcgtgctg gaaagcgacg ttgatggatt aatgcagtcg gtaaaactga acgctgcgca
    32881 ggcaaggcag cagcttcctg atgacgcgac gctgcgccac caaatcatgg aacgtttgat
    32941 catggatcaa atcatcctgc agatggggca gaaaatggga gtgaaaatct ccgatgagca
    33001 gctggatcag gcgattgcta acatcgcgaa acagaacaac atgacgctgg atcagatgcg
    33061 cagccgtctg gcttacgatg gtctgaacta caacacttat cgtaaccaga tccgcaaaga
    33121 gatgattatc tctgaagtgc gtaacaacga ggtgcgtcgt cgcatcacca tcctgccaca
    33181 ggaagtcgaa tccctggcgc agcaggtggg taaccaaaac gacgccagca ctgagctgaa
    33241 cctgagccac atcctgatcc cgctgccgga aaacccgacc tctgatcagg tgaacgaagc
    33301 ggaaagccag gcgcgcgcca ttgtcgatca ggcgcgtaac ggcgctgatt tcggtaagct
    33361 ggcgattgct cattctgccg accagcaggc gctgaacggc ggccagatgg ggtggggccg
    33421 tattcaggag ttgccgggga tcttcgccca ggcattaagc accgcgaaga aaggcgacat
    33481 tgttggcccg attcgttccg gcgttggctt ccatattctg aaagttaacg acctgcgcgg
    33541 cgaaagcaaa aatatctcgg tgaccgaagt tcatgctcgc catattctgc tgaaaccgtc
    33601 gccgatcatg actgacgaac aggcccgcgt gaaactggag caaattgctg ctgatatcaa
    33661 gagtggtaaa acgacttttg ctgccgcagc gaaagagttc tcgcaggatc caggttctgc
    33721 taaccagggc ggtgatctcg gctgggctac accagatatt ttcgatccag ccttccgtga
    33781 cgccctgacc cgcctgaaca aaggtcaaat gagtgcaccg gttcactctt cattcggctg
    33841 gcatttaatc gaactgctgg atacccgtaa tgtcgataaa accgacgctg cgcagaaaga
    33901 tcgtgcatac cgcatgctga tgaaccgtaa gttctcggaa gaagcagcaa gctggatgca
    33961 ggaacaacgt gccagcgcct acgttaaaat cctgagcaac taatggttaa aacccaacgt
    34021 gttgtgatca ctcccggcga gcccgccggg attggcccgg acttagttgt ccagcttgca
    34081 cagcgtgagt ggccggtcga actggttgtt tgtgccgatg ccactctcct taccgaccgg
    34141 gcagcgatgc tcggtttgcc gctcaccctc cgcccttatt cccccaactc ccctgcacaa
    34201 ccgcaaacta cgggcacatt aacgctactt cctgtcgcgc tacgtgaatc tgtcactgcg
    34261 gggcagttag cgattgaaaa tgggcattac gtggtggaga cgctggcgcg agcgtgtgat
    34321 ggctgtctga acggtgaatt tgctgcgctg atcacaggcc ccgtgcataa aggcgtcatt
    34381 aacgacgcaa gcattccgtt taccggtcat accgagtttt tcgaagagcg ttcgcaggcg
    34441 aaaaaagtgg tgatgatgct ggcgaccgaa gaacttcgcg tggcgctggc aacgacgcat
    34501 ttaccgctgc gcgatatcgc agatgctatc acccctgcac ttttgcacga agtgattgct
    34561 attttgcatc acgatttgcg tacaaaattt ggtattgccg aaccgcgcat tctggtctgc
    34621 gggctgaatc cgcacgcggg cgaaggcggt catatgggta cggaagagat agacaccatt
    34681 attccggtgc tcgacgagct gcgggcgcag gggatgaaac tcaacgggcc gctgcctgcc
    34741 gataccctgt ttcagccgaa atatctcgat aacgccgacg ccgtgctggc gatgtaccac
    34801 gatcagggtc ttcccgtgct aaaataccag ggcttcgggc gcggtgtgaa cattacgctg
    34861 ggcctgccct ttattcgcac atcagtggac cacggcaccg cgcttgaact ggcgggacgt
    34921 ggcgaagccg atgtcggcag ttttattacg gcgcttaatc tcgccatcaa aatgattgtt
    34981 aacacccaat gaataatcga gtccaccagg gccacttagc ccgtaaacgc ttcgggcaaa
    35041 actttctcaa cgatcagttc gtgatcgaca gcattgtttc tgccattaac ccgcaaaagg
    35101 gccaggcaat ggtcgaaatc ggccccggtc tggcggcatt gaccgagccg gtcggcgaac
    35161 gtctggacca gctgacggtc atcgaacttg accgcgatct ggcggcacgt ctgcaaacgc
    35221 atccattctt aggcccgaaa ctgacgattt atcagcagga tgcgatgacc tttaactttg
    35281 gcgaactggc cgagaaaatg ggtcagccgc tgcgtgtttt cggcaacctg ccttataaca
    35341 tctccacgcc gttgatgttc catctgttta gctatactga tgccattgcc gacatgcact
    35401 ttatgttgca aaaagaggtg gtgaatcgtc tggttgcagg accgaacagc aaagcgtatg
    35461 gtcgattaag cgtcatggcg caatactatt gcaacgtgat cccggtactg gaagttccgc
    35521 cgtcagcctt tacaccacca cccaaagtgg attccgccgt cgtgcgcctg gtccctcatg
    35581 caacgatgcc tcacccggtt aaagatgttc gcgtgttgag ccgcatcacc accgaagcct
    35641 ttaaccagcg tcgtaaaacc attcgtaaca gcctcggcaa cctgtttagc gtcgaggtgt
    35701 taaccggaat ggggatcgac ccggcgatgc gagcggaaaa tatctctgtc gcgcaatatt
    35761 gccagatggc gaactatctg gcggagaacg cgcctttgca ggagagttaa cgatgatcaa
    35821 ttcgccccga gtgtgtattc aggttcaaag cgtctacatt gaggcacaat cttcacctga
    35881 taatgaacgt tacgtttttg cttataccgt aaccatacgc aatctggggc gagcgccagt
    35941 gcagttgttg gggcgttact ggctgatcac caatggcaat ggccgtgaaa ccgaagtcca
    36001 gggcgaagga gtggttggcg tccagccact tatcgcgcct ggcgaagagt accagtacac
    36061 cagcggtgca atcattgaaa ccccgctggg caccatgcag ggtcactacg aaatgatcga
    36121 tgaaaatggc gtccctttca gcatcgacat tcccgtattc cgactcgccg ttcccacact
    36181 cattcattaa aagaatatgg cgacatacct tattggcgac gttcatggtt gttacgatga
    36241 actgagcgca ttgctgcata aagtagaatt tacccccggg aaagataccc tctggctgac
    36301 gggcgatctg gtcgcgcgcg gcccgggttc gctggatgtt ctgcgctatg tgaaatcctt
    36361 aggcgacagc gtacgtctgg tgctgggcaa tcacgatctg catctgctgg cggtatttgc
    36421 cgggatcagc cgcaataaac cgaaagatcg cctgacaccg ctgctggaag cgccggatgc
    36481 cgacgagctg cttaactggc tgcgtcgcca gcctctgctg caaatcgacg aagagaaaaa
    36541 gctggtgatg gcccacgcag ggatcacgcc gcagtgggat ctgcagaccg ccaaagagtg
    36601 cgcgcgcgat gtagaagcgg tgctgtcgag tgactcctat cccttctttc ttgacgctat
    36661 gtacggcgat atgccaaata actggtcacc ggaattgcgg gggctgggaa gactgcgttt
    36721 tatcaccaac gcctttaccc gtatgcgttt ttgcttcccg aacggtcaac tggatatgta
    36781 cagcaaagaa tcgccggaag aagcccctgc cccactgaaa ccgtggtttg cgattcctgg
    36841 ccctgtcgct gaagaataca gcatcgcctt tggtcactgg gcatcgctgg aaggcaaagg
    36901 tacgccggaa ggtatttacg cgctggatac cggctgctgc tggggtgggt cattaacctg
    36961 cctgcgctgg gaagataaac agtattttgt ccagccgtcg aaccggcata aggatttggg
    37021 cgaagcggcg gcgtcttaaa cacagcctga tacaggaagg ccggataaga cgcgaccggc
    37081 gtcgcatccg gcactagccg taaattctat acaaacttac cgccgctcca gaatctcaaa
    37141 gcaatagctg tgagagttct gcgcatcggc gtcgtggaat tcgctgaata ccgattccca
    37201 gtcatccggc tcgtaatccg ggaaatgggt gtcgccttcc acttctgcgt cgatatgcgt
    37261 cagatacagt ttttgcgctt ttggcaggaa ctgctcataa acgcgaccgc cgccaatcac
    37321 catgatttct ggtacgtcac cacacgccgc aatggcttca tccaccgatt tcacccacgt
    37381 tacgcgatcg tccgtacccg gttgactgct gaggataata tttttgcgtc ctggcaacgg
    37441 acgaccgatt gattcccaag tatggcggcc cataatcacg ggtttattta aggtgttgcg
    37501 tttaaaccag gcgagatcgg caggcaggtt ccacggcatg gcgttttcca tgccgataac
    37561 gcgatctacc gctaacgccg caatcagact gatcattgag atttcccgat aaaaaaattg
    37621 tcgccactat acgtaaagcg taaaccgtcg tcgactggtg cgaggatgat gttgaggaaa
    37681 attttatatt ctgctggcga gtccacgctc tccccctgga ctcgccgcat tacaatgaaa
    37741 caaaaacaaa cagttagctg taaagtgtga tttacgtcac tctttattaa gatgagggtt
    37801 tcgtttccgg ttcatccgcc atgttgccgg tatgtttacc ttcttctgtt ccctgccagc
    37861 catgtcgttg aattaatgac agatgttcgc ggtcctcggt gatgatctca cttaacatcg
    37921 cgctggtgcg tttatagacc gccgcgcggg ctttggtgtc attctcaacc attgccatct
    37981 cttccaccat ctgaatatta aagcggcgga acacatcggc acgttctcgc gcttcatacg
    38041 gccccagacc taaactttcc agcgccagac gcccggtttt cagcgcacct tcaaaggttt
    38101 cacgctccgg cttttcaacg cctgcctgac gcaaacgaat gtagtgatcg acatcgcggg
    38161 cgcgggcaat aatctgcaaa tgcgggaaat gttctttcac catctccgtc agttgcaggt
    38221 tggtttgcgg atcgtcaata gcgttaatca gcacttccgc tttcgccgct ccggcagatt
    38281 ccagtaaatc catccgcgtg gcatcgccat aaaacacttt cataccaaat ttacgcagcg
    38341 tttcgatatg gtccggatcg tgatcgagca ccaccatttt caccccgctg gagagcagta
    38401 aacgtccggt aatctgtcca aaacgaccga atccggcgat aatcacgcgc ggctgttctt
    38461 catcgatctc atcggcttca cgcgcctcct ccgtagaaga ttgctcaagg cgattgagga
    38521 tcaccagcag aatcggcgtt gctgccatcg acagcgccac cgccagggtc agcgatttcg
    38581 cccactccgg ctccagcaca ttcgccatct gcgccgcacc aaataccaca aaggcaaact
    38641 cactgccctg ccctaacaac accgcaaacc agcgacgctg tttatttggc acctgcaacg
    38701 gtcgggcaat cagccacagc atggcgattt tgatgatgag gaaaccgagc agcaaaatga
    38761 caatgcgcaa tgggttttca atcagcgtgc caaagtctat cgacatgcca acaccgatga
    38821 aaaacagccc caacagcaaa cctttaaacg gttcgatatc gctttccaac gcatgacggt
    38881 attccgagct tgccagcagt acgcccgcca gaaacgcgcc catcgccatc gacaagccga
    38941 cctcttccag cagcaaacca aagccaaaca caaggaataa cgccacggca ctaaacactt
    39001 cccgcaagcc agagcgggca acaaaacgca gcgccggacg cgtaacatag cgccccagca
    39061 ataccaccag taccagcgca cccgccactt ttaacgccga gagagcaaat gcgcccatcg
    39121 tcgtcgaggc actgctcgcc gccagtagcg ggatcatcgc caccagcggg attgccgcga
    39181 tatcctggaa cagcagcact gcaaaggcac tgcggcccat ttgcgtcacc atcagattgc
    39241 gttcattcat cgcctgcatg gcaatcgccg ttgatgagag cgccagcgtc ataccgatca
    39301 attccgcgac ctgccagcgc agcccaagta acatacagaa cagccccagc aggccaccgc
    39361 aaatcaccat ctgcaatgcg ccaccgccga acactgccgc acgcagcttc cacagccttt
    39421 gtggatcgag ttcgaggccg ataataaaca gcatcagcac caccccaatc tcggcaaagt
    39481 gcagaataga ttcggcatcg gtcaccagtc gcagccccca cgggccaata atgcagccgg
    39541 cgatcaggta gccaagtacc gatcccagac caagacgtac cgcaatgggt acaatcagcg
    39601 ccgccgatcc gaggtagatc agcgcctgaa ccagcgtatg actatccatg atgggcctcc
    39661 tgccattcca gcagacgttg cttatagtgg cgcgcctgcc cttcgagggt ttcgtcgtca
    39721 caaataaagg tgcagtgcat ggcaaacggt ggcagccagt tcagcccgca gtagattgcc
    39781 gtcgcctgta gcggctgcga cagcacatca aagcccggat gcgcaccaat ttcaaaatgg
    39841 ctttccccgc cgccggtcgt caccgcccac agcaaatgtt tgccatgcag cgccgtgccg
    39901 ccgtgaccgt aagcccagcc gtgcgacaaa actttatcga tccaaagttt aagaagcgga
    39961 ggaatgctgt accactgcat cgggtgctgc cagacgatca gatcggcgcg agagagcgcc
    40021 tcctgctcgg cggcaatatc gatattgaag tcaggataga gttgataaag agagcgaatt
    40081 tcgacgcctt ccagcgtcct tgcctgttca agcatccgtt tattcgcatg ggaatgatgc
    40141 ggatacggat gcgcataaat gatgaggatc atagttatta gcccgcgtgt ttacgccttt
    40201 gttttcccac aaagtgtagt catatattca cgcggcttat agtcaataat attgattcag
    40261 aaaagagaga aatctgaaca aataatccca ttctctgccc gcggatcgtc atattgctgg
    40321 tctgcgccag tgacattcct cgagtctccg gggcaaacgc tacggaaatc aacaagccaa
    40381 acagcgagat acccgccccc attagcatcg tgttactgat accgtaatta ttgataaata
    40441 tcggcagtgc ccaggtcgaa acaatggtgc caatacgact taaggacata atcacgccca
    40501 cggcagaggc gcggatatcc gtcgggaaga gttcattagg atagagccac tgcaaattac
    40561 ccggcccgcc agagaaaaag gcatacaccg caaaggccat cactaccagc cagatcccca
    40621 tatccgggat tagccccaaa accgccagcg ccagcgtcat catggcaaag ctgccaatca
    40681 acaatggacg ccgtccggca gtgtttagcc acagcatcgg cggaatacag ccgagcataa
    40741 agaacaggct aatcaccaca ttccctagtg ccgcgttttt gccaaccccc aatcccaacg
    40801 aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat tgggatcacc tggcaggtcc
    40861 agatggcggc aacaaacaga acaaaaggaa aatggcggcg attaaacaga tcgcgaaaac
    40921 gggtttgctg cggctgctct tcatcgaaag ccaccggttc gccaaacagt tttatcatca
    40981 tctcttcgca ctcttttact cgccctttgc gtaataacca gcggggagat tctggcagtt
    41041 cgaatcgacc aatcaaaatc aacaaacaag ggatcgccgc gctacccagc atccagcgcc
    41101 aaccaccttc cacatcataa agccagtagc cgaccagatc ggcgcaggtc gcgccgacat
    41161 accacatcgc ggcaataaag ctgatggaaa acgcccgctg acgggtactg gagaactcgg
    41221 tgatcatcga agtggcgatg ggataatctg caccgatgac aatgccgatg agtacccgca
    41281 tcaccaacag ttcgacgggg gatgaaacac acatcgtcgc caccgatatc acgccgatgg
    41341 cgatgatatc aatgaggaac attttgcgcc gtccgacttt atcggagata tagccaaaca
    41401 gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc cagcaagcca atccagtcag
    41461 cgtccagttt cagcgccgga gtcagttgct ccagcgccac gccaatcatt accagtacat
    41521 aaccatccag aaacggacca ccgcttcccc acagcaaaat gcggcggtga atagaggaga
    41581 atttgagatc gtcaaagttt ctggacggtt gcatgggtta ttcctgtttt tttgcaatga
    41641 gagtcagtac ggcgttacgt atcaaaccaa catcagccgt aacggaactc cacaccaaag
    41701 gtgccgcgcg ggtattccca ctgttccagc gccgacccca gccccagaat gcgacaggtg
    41761 ccgcactcca gacatccggc gtaatcgaag cgcacactgc cgtcatcctg cttcttgtac
    41821 agacctgcgg ggcacgcttt caccagcagc tccagcacct gtttatcagc atcggccttc
    41881 acaacaatgt gcggatgctc ttcatcgaca ttgaatttat tgacgcccag tttgacgtcc
    41941 acattgacgg gagaagtcat aaaacggtca ctcctttcat gccatccttg atcagattga
    42001 tgaagcccac tttcttgccg tggcggagga ttttcttgcg catcagttcc ggcgcactgc
    42061 cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag ttccgggttg ccgctaaaca
    42121 tgcgtgggtt atcaaggaac gccggtagtt tctggtacat gcgcatatcg cgcagcgggc
    42181 cactctccag atgctgacga tattccgcga gtttttgctt actgaaatcg tcgcttttca
    42241 tcgctgaaag cacggttttt gctgcagctt ccccggcggc aatcgccaga tccataccgc
    42301 gaatggtaaa accgaggttc atacacattc cggcggcatc accggcaatc aatacgccgt
    42361 caccgaccag ctccggcagc atgttgatgc ctgcttccgg cactacgtga gcggaatatt
    42421 ccaccagctt gccgcccgcg atcagcggtg caacggccgg atgctgtttg aaatcttcca
    42481 gcatttgcgg caccgatttt ttcgcgtcat gcagatgatg cagaccacaa accagcccca
    42541 gcgacagggt attttcattg gtataaagga agccgccgcc catcaggcca tcggtgggtg
    42601 atcccgcaaa cagacaagcc gcgccctgat taccctgcaa ctgaaaacgg tcttcgatta
    42661 ccgacttcgg taactcgatc agttccttca cgccaaccgc cacatccgtc ggtttaacgc
    42721 gttttgccat ccccaacttt tcggcaagga tggagttcac cccatcagca aggagcaccg
    42781 ttttcgcttc aatcacatcg ccatcggctt ctacaccgac gactttgcca tcgcgctgta
    42841 cgaggttatc gacgcggatc ccggtaatta actgcgcgcc cgcttcttcg gcctgctcca
    42901 tcaaccaggc atcaaattta ctgcgcaaaa cggagtaaga acgctgggat ggcgaggttt
    42961 cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc cgtcataaac gcgagttttt
    43021 catgggtgat caggcgttct acgggggcgg aatcggcgaa acctggaata atgtgttcca
    43081 gactgtgggc atagagacgc ccgccagtaa cgttcttggc acctgcggaa ttgccacgct
    43141 cgataactaa cacttgcgcc ccttcgcggg cgagcaccag tgcggcaacc gaaccggcaa
    43201 gccctgcacc gacgatgatg gcgtcaaaga tatcttcgga cataagagct ccctgtcagc
    43261 ggcagagccg taatgcagcc ctgccagagt ggatcaacgc gctaaagctg cggtcagtgc
    43321 cggaaggatc ttcacggcat cgccaacaat gccgtaatcc gcgtactgga agatcggcgc
    43381 atttttatct ttattgatgg cgaaaatggt ttgcgacgcg ttagcgccaa ccatgtgctg
    43441 gatctacccg gagatcccca ccgccaggta cagttcaggt ttcagcatca ggttggagat
    43501 accgacatag cgttcgtgct ccatccattt ttcgttttct gccaccggac gagaacaggc
    43561 caactccgca cctatcgcct tgcaaagctg ttctgccagc gcaatgttct ctttgctgcc
    43621 aatgccgcga ccgacgctga ccaccagacg ggctttgtcg agatcgacgc tgttgctctg
    43681 gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga gcctgccact ccacggtgtg
    43741 cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg aacgtgccgc tgctgatggt
    43801 cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc gccagaccgc cgtaaaccat
    43861 gtgtttcact gttgctttac cgtcctgtac gctgacggtg ctggcatcgt tagacaccgc
    43921 cgctttaagg cgataaccca gttttgccgc cagtaatttg ccgcgacggg tgtttggcaa
    43981 cagcaccaga ccgtcagcgc cgtgctggcg aatagtgtca gccatgacac cggcgtaatc
    44041 ttcgatcatc cgatcgtccg gtttgccgtt taatttccag acatgattag cgccgagctg
    44101 gattgcctgt gcgccgtcgg catcattgag gacaaaggca ttgatttgat tagctaaagc
    44161 ctgcgcaccg ttcatcagtt ccggcagacg agagggggta tcgctgaata cccagacttg
    44221 agaaaacgtg ttcatagcat cccctgtaat taaatgactt tgcgaagatt ttcagcaaat
    44281 gcggcgatct gttcttcgcc gtcgccttca atcacgatgc gctgacgctc gcgctgtttc
    44341 ggcgcggcaa cctgttgttc agaccaggcg tccacggcgt taaaaccaat atccgccgcc
    44401 gaccatacct ggactggctt tttcgccgcg ccgagaatgg ctttcatcga aggaatttgt
    44461 ggggagttga tatcagtgga aacagcaaca accgcaggca gcggaatgct taaggtttcg
    44521 gtttcatctt ccagttcgcg ctcaacggtg agggtatctg ccgtcaggga gataattttg
    44581 ctgacgccgt taactgccgg aatattgagg atttcgccca ccagcagacc aacctgctgg
    44641 gcataaaggt cggaagaacc atcgccacag aggatcagat caaagcctgc tttctgggcg
    44701 gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct gctcgaactg gtcatcaatc
    44761 accacaatca gttcatccgg gccgcgcgat agcacatctt tacgcccttt ggcgttggtc
    44821 agggctttac cgcccacact taaggctgtc acctgcgcct ctgctgcctg ttgctttagc
    44881 tggcaagccg cttcaatagc gttgagatcg tattggctta ttttggcatc ggctttgctg
    44941 aagtctaatg aaccatcagc attattgacc gcaatatcct gttcatcagg cacgcactta
    45001 tagcaagtaa taatcttcat tgcatctcca gaaatcatga aggaaacgtc gttcatatcg
    45061 gtggtgttaa ttacagatat cacgcccttt aatttggaaa taaaacttta atcaccaata
    45121 ttgaaaatgt cacacgcaga taaatccaac tttcaatatt gttaagttcc tcaccaatat
    45181 tgaaaacatg gcgtagcaaa aagaaatttt caatattgtt ttatagatca ccaatattga
    45241 aagtcagatg ctattttttt acccacaaaa gaatataaca aactgattaa taaccaaaat
    45301 taacgaaaaa cgccctctgt tcactcgaaa caaaaatgtg acacaaatca cagaatacag
    45361 cttattgaat acccattatg agttagccat taacgcgtcc acgaggttaa taataattat
    45421 attaaatgtt aacaaaaata aaacaaacgg gaacgcaaaa taaatattcg ttttcacagt
    45481 ggaattaact catgaagaat gaaaagagaa aaacgggaat agaaccgaag gttttctttc
    45541 cgccattaat aatcgtcggc atactttgtt ggcttacagt cagagatctg gatgcggcga
    45601 atgtcgttat taatgctgta ttcagttacg tcaccaatgt atggggatgg gcatttgaat
    45661 ggtatatggt ggtgatgctt ttcggttggt tctggctggt gtttggcccg tatgccaaaa
    45721 agcgtttagg taacgaaccg ccagaattta gcaccgccag ttggatcttt atgatgttcg
    45781 cctcctgtac gtctgctgcc gtactgttct ggggatcgat tgagatctac tactacatct
    45841 ccaccccgcc gtttggctta gaaccgaact cgacaggagc gaaagagttg gggctggctt
    45901 acagcttgtt ccactgggga cctctgccgt gggccactta cagcttcctt tcagtcgcct
    45961 tcgcttactt cttctttgtc cgcaaaatgg aagtgattcg ccccagctcg acactggtgc
    46021 cgctggtagg tgaaaaacac gccaaagggt tgttcggtac catcgtcgac aacttctatc
    46081 tcgtcgcctt gatcttcgcg atgggtacca gtctgggcct tgccacgccg ctggtgaccg
    46141 agtgtatgca atggttgttt ggcattccgc ataccctgca actggacgct atcatcatta
    46201 cctgctggat tatcctcaac gccatttgcg tcgcttgcgg tctgcaaaaa ggggtacgta
    46261 tcgccagtga cgtgcgtagt tacctgagct tcctgatgct gggttgggtg ttcatcgtca
    46321 gcggtgccag cttcatcatg aactacttca ccgattcggt ggggatgttg ctgatgtatc
    46381 tgccgcgcat gttgttctat accgatccca tcgctaaagg cggcttcccg cagggctgga
    46441 ccgtgttcta ctgggcatgg tgggtgattt acgccatcca gatgagtatc ttcctcgccc
    46501 gcatctcccg tggtcgtact gtgcgtgaac tgtgcttcgg catggtgatg gggctgacag
    46561 catccacctg gatcctgtgg accgtgctcg gtagtaacac tctgctgttg atggataaaa
    46621 acatcatcaa cattccaaat ctgatcgaac agtacggtgt ggcgcgcgcc atcatcgaaa
    46681 cctgggccgc tctgccactc agcaccgcca ccatgtgggg cttcttcatc ctctgcttta
    46741 ttgccaccgt tacgctggtt aacgcctgct cttataccct ggcgatgtct acttgccgcg
    46801 aagtacgcga tggtgaagaa ccaccgctgc tggtgcgtat cggttggtca attctggttg
    46861 gcattatcgg tattgttctg ctggcgctcg gcggcctgaa accgattcaa accgccatta
    46921 tcgccggagg atgcccgctg ttcttcgtca acattatggt gacgctctcc tttattaaag
    46981 acgcgaaaca gaactggaaa gattaattaa cccccaaaat atcaagaggt tgaaagatgg
    47041 attttaattt aaatgatgag caggaactgt ttgtcgccgg tatccgcgaa ctgatggcca
    47101 gcgaaaactg ggaggcctat tttgccgagt gcgaccgtga cagcgtctac ccggaacgtt
    47161 ttgtcaaagc actggcggat atgggtatcg acagtctgct gatccctgaa gagcacggtg
    47221 gtctggacgc ggggtttgtt actctcgccg ccgtgtggat ggagctggga cgtctggggg
    47281 caccaaccta tgtactgtac cagctgccgg gcgggttcaa taccttcctg cgcgaaggca
    47341 cacaagagca gatcgacaag attatggctt tccgcggcac cggtaagcag atgtggaact
    47401 cagcgattac cgaaccgggc gcgggctccg acgtgggtag cctgaaaacg acttataccc
    47461 gtagaaatgg taagatttat cttaatggta gtaagtgttt tattaccagt agcgcctaca
    47521 ccccgtacat cgtggtgatg gcgcgcgacg gggcttctcc ggacaaacct gtctacaccg
    47581 aatggtttgt tgatatgagc aaaccgggca tcaaagtgac caaacttgag aagctcggtc
    47641 tgcgtatgga tagctgctgt gaaatcacct ttgacgatgt ggaactggac gagaaagaca
    47701 tgttcggtcg ggaaggtaac ggctttaacc gcgtcaaaga agagttcgac catgaacgtt
    47761 tcctggtagc cctgaccaac tacggtacgg cgatgtgcgc ctttgaagat gcggcgcgct
    47821 acgccaacca gcgcgtgcag tttggcgagg ctattggtcg tttccagttg attcaggaaa
    47881 aattcgccca catggcgatc aaattaaact ccatgaaaaa catgctgtat gaagcagcgt
    47941 ggaaagcaga caacggcacc atcacctctg gcgatgcagc gatgtgcaaa tacttctgcg
    48001 ccaatgcggc atttgaagtg gtggatagcg caatgcaggt gctgggcggt gtcgggattg
    48061 cgggcaacca ccgcatcagc cgcttctggc gtgacctgcg tgtagaccgc gtttccggag
    48121 gatctgacga aatgcagatc ctgacgctgg gtcgtgcggt gctgaagcaa taccgctaag
    48181 tccgtgcgct gcctgatgcg acgctgacgc gtcttatcag gcctacggga gcacgaatgt
    48241 aagccggata aggcgtttac gccgcatccg gcagtcatgc gctacataaa tttttcagga
    48301 gctaataatg gatcatctac ccatgccgaa attcggaccg ctggccggat tgcgcgttgt
    48361 cttctccggt atcgaaatcg ccggaccgtt tgccggacaa atgttcgcag aatggggcgc
    48421 ggaagttatt tggatcgaga acgtcgcctg ggccgacacc attcgcgttc aaccgaacta
    48481 cccgcagctc tcccgccgca atttgcacgc gctgtcgtta aatattttca aagatgaagg
    48541 ccgcgaagcg tttctgaaat taatggaaac caccgatatc ttcatcgaag ccagtaaagg
    48601 tccggccttt gcccgtcgtg gcattaccga tgaagtactg tggcagcaca acccgaaact
    48661 ggttatcgct cacctgtccg gttttggtca gtacggcacc gaggagtaca ccaatcttcc
    48721 ggcctataac accatcgccc aggccttcag tggttacctg attcagaacg gtgatgttga
    48781 ccagccaatg cctgccttcc cgtataccgc cgattacttt tctggcctga ccgccaccac
    48841 ggcggcgctg gcagcactgc ataaagtgcg tgaaaccggt aaaggcgaaa gtatcgacat
    48901 cgccatgtat gaagtgatgc tgcgtatggg ccagtacttc atgatggatt acttcaacgg
    48961 cggcgaaatg tgcccgcgca tgagcaaagg taaagatccc tactacgccg gttgcggtct
    49021 gtataaatgt gccgacggct acatcgtgat ggaactggtg ggcattaccc aaattgaaga
    49081 gtgctttaaa gatattggcc tcgcacatct actcggtacg ccggaaatcc cggaaggcac
    49141 tcagcttatc caccgtatcg aatgccctta cggcccactg gtagaagaaa aactcgatgc
    49201 ctggctggcg gcacatacca tcgcagaagt taaagaacgc tttgccgaac tgaatatcgc
    49261 ctgtgccaaa gtactgaccg taccggaact ggaaagcaat ccacagtatg ttgcccgtga
    49321 atcaatcact cagtggcaaa cgatggatgg tcgcacctgc aaagggccga acatcatgcc
    49381 gaaattcaaa aataaccccg gacaaatctg gcgcggaatg ccctcacatg gcatggacac
    49441 ggctgccatt ttgaaaaata tcggctacag cgaaaacgac attcaggagt tggtcagcaa
    49501 aggtctggcc aaagttgagg actaaacgcg tggcgtcagt cggcttgcct gactagcgcc
    49561 cgcccaataa caaacgaaca aaaatggata gaggtgcaat ggatatcatt ggcggacaac
    49621 atctacgtca aatatgggac gatctggcgg acgtttacgg tcataaaacg gcgctgattt
    49681 gtgaatccag cggcggagtc gttaaccggt atagttatct tgagttaaat caggagatta
    49741 accgcacggc aaacctgttt tatacgctgg ggattcgcaa aggcaacaag gttgcactac
    49801 atctcgacaa ctgcccggaa tttatctttt gctggttcgg gctggcaaaa attggcgcga
    49861 ttatggtgcc gattaacgcc cgcctgttac gcgaggaaag cgagtggatc ttgcaaaata
    49921 gccaggcgtg cctgctggtg accagtgcgc aattttatcc tatgtatcaa cagattcagc
    49981 aggaagatgc cactcaattg cggcacattt gcctgacaga tgtggcactt cccgctgatg
    50041 atggagtgag ttcatttact caactgaaaa atcaacaacc tgccaccttg tgctatgcac
    50101 cgccgctatc gactgacgat acggcggaaa ttctcttcac ctccggcacc acctcccgac
    50161 cgaaaggtgt ggtgattacc cattacaacc tgcgcttcgc tggatattac tccgcctggc
    50221 agtgtgcact gcgtgacgat gacgtctaca tgacggtaat gcctgcgttt catatcgatt
    50281 gccagtgtac tgcggcgatg gcggcgtttt ctgccggggc cacctttgtg ctggtcgaga
    50341 aatacagcgc ccgcgccttc tggggacagg cgcagaagta ccgcgccacc attaccgaat
    50401 gtattccgat gatgatccgt acgttgatgg tgcagccgcc ttcagcgaac gatcggcaac
    50461 accgcctgcg ggaagtgatg ttttatctca acttgtcgga gcaggaaaaa gacgcatttt
    50521 gtgaacgctt cggcgttcgc ttgctgacgt cttatgggat gacggaaacc attgtgggca
    50581 ttatcggcga tcgccctagc gataaacgac gctggccgtc gattggtcgg gcggggtttt
    50641 gctacgaagc ggagatccgc gacgatcaca atcgcccgct cccggcaggt gagatcggtg
    50701 aaatctgtat taaaggcgta ccagggaaaa ccatcttcaa agagtatttt ctcaacccga
    50761 aagccactgc gaaagtgctg gaagccgacg gctggctaca taccggcgat accggatacc
    50821 gcgacgaaga aggctttttt tatttcgtcg atcgccgctg caatatgatc aaacgcggtg
    50881 gcgagaatgt ctcctgcgtg gagctggaaa atatcatcgc cacccatccg aaaattcagg
    50941 acatcgtggt tgtgggtatt aaagattcga ttcgcgatga agccatcaaa gcatttgtgg
    51001 tgctgaatga aggtgaaaca ttgagcgaag aggaattttt tcgcttctgc gaacaaaata
    51061 tggcgaaatt taaagtgccc tcttatctgg agatcagaaa agatctgcca cgtaattgct
    51121 cggggaaaat aattagaaag aatctgaaat aaatgaaacg tcagggaact accctgccag
    51181 ctaataatca cgccctaaaa caatatgcat tttttgcagg gatgttgtcg tccctaaaaa
    51241 agcaaaaatg gagaaaagga atgagtgaat cattacatct gacccgcaat ggatcaattc
    51301 tggaaattac ccttgatcgc ccaaaagcga atgctattga tgcaaaaacc agctttgaaa
    51361 tgggcgaagt atttctaaat ttccgtgacg atccgcaatt acgtgtcgcc attattaccg
    51421 gtgccggaga gaaattcttt tccgcgggct gggatttaaa agcggcagca gaaggcgaag
    51481 caccggatgc tgactttggc ccgggtggtt ttgcgggatt aaccgaaatt ttcaatctcg
    51541 acaaaccagt tatcgcagct gtgaacggct acgcctttgg cggcggtttc gaactggcgc
    51601 tggcggcaga ttttattgtt tgtgccgata acgccagctt cgccctgccg gaagccaaac
    51661 tgggcatcgt tcctgacagc ggcggtgtgc tgcgtctgcc gaagatcctg ccgcctacca
    51721 tcgtcaatga aatggtgatg accggcagac gaatgggcgc agaagaggcg ctgcgttggg
    51781 ggatagtcaa ccgcgtggtt agccaggcgg aactgatgga taacgcccgc gaactggctc
    51841 agcagctggt taacagcgcc ccgctggcga ttgcggcgct gaaagagatc taccgcacca
    51901 ccagcgaaat gccggtagaa gaagcgtatc gctatattcg cagcggcgtg ttgaaacact
    51961 atccatcggt tctgcattcg gaagatgcca ttgaagggcc gctggcgttt gccgagaagc
    52021 gcgatccggt gtggaaagga cgttaacaac cgtgagctat tacgcctttg aggggttaat
    52081 tccggtggtt cacccgacgg cgtttgtcca tcccagtgcc gtcttgattg gcgatgtgat
    52141 tgtgggagcc ggtgtctaca tcggcccact cgcctcactg cgtggtgact acgggcggtt
    52201 gatcgtgcaa gcgggagcca atattcagga tggctgcatt atgcatggct actgcgacac
    52261 tgacaccatc gttggggaaa acggccatat cgggcatgga gcgatcctgc atggttgtgt
    52321 gattggtcgc gatgcattgg tcgggatgaa cagcgtaatt atggatggcg cggtcattgg
    52381 cgaagagagc attgttgccg caatgagctt tgtcaaagca ggctttagtg gcgagaaacg
    52441 ccagttgttg atgggtacgc cagcccgcgc tgtacgcagt gttagtgacg acgagttaca
    52501 ctggaaacgg ttgaatacca aagagtatca ggatcttgtc gggcgctgtc atgcatcgtt
    52561 acatgaaacg cagccactga ggcaaatgga ggaaaatcgc ccccgtttgc aggggacgac
    52621 ggatgtgacg cctaaacggt gatcttgccg gatgcgacct ggttgctgga taagatgcta
    52681 caacatcgta tctggcaagc aggacgcccg gtatcaacga gataaaatta acgacgcata
    52741 ctctttgaca acatcatctg ccacttctgc tcgcggttaa gttccgtgag cggaacggca
    52801 ggaatacgcg gtgtctttct tacgccgact aatttttccc gactgttatt gcgcaggcgc
    52861 gcgtaaattt gctcatcgat atcgaccact ttaaccagtc gctgacactg gcatccccgc
    52921 ccttccagct tattagggat catcttaact tcgcagctta tttctgtgac ttccgacaga
    52981 atataagtca gggtattaac cgccttgctg tgttcaatat cgaaatgaat agagatctct
    53041 cttgctatca cccaccgatt ttcagccatc atccattcgg cgattaacaa gtagagtggt
    53101 ttttcaacat atccttcaca catacgaact ccagtttcac aaattaaaag tctggctatg
    53161 actcgttttg aagcaattaa acaaggccat attaaaattg tggatatatc gatagtatgc
    53221 aacttcaccg tcgataagtg tgagctaaat cctgcttatg tcatcaaaaa tatcgattcc
    53281 ccaaaagatt tacttaatgg gcaaaaaaaa acggtcctca tcagagaacc gtattagtta
    53341 ttcgatcaaa ttagcggatg aaaaatatcc gccacgacac gctattattt gatctgtgcg
    53401 tgcatttcct gcaccgaaat tactttttca gtcgcatcgg cattcagcgc catcgcggta
    53461 gcgaaaccac cgttcagggt ggtgtcataa tgcactttat attgcagcgc actgcgacgg
    53521 atcacgcggg agtcttcaat cgcacgacgg cctgaggtgg tgttgatgat gtaggtatat
    53581 tcgccattct tgatacggtc ctgaatgtgc ggacggcctt catgcacctt gtttaccaga
    53641 cgcggattga tacccgcttc gcccagcaca atcgccgtgc cgtgggtcgc atccagctcg
    53701 aagccctgtt tcagcagttt tgccgccagg tccaccacgc gttctttatc gccttcgcgc
    53761 acggaaagca gcgcacgacc gtgtttcttc atggtggagt tgctgcccag ctgcgctttg
    53821 gcgaacgctt cagcgaaggt gcggcccacg cccatgactt ccccggtaga gcgcatttct
    53881 ggccctaaca gcgggtcaac gcctgggaat ttattgaacg gcagcaccac ttctttcacc
    53941 gagtagtacg gcggaataac ttctttggtt acgccctgct cagccagcga tttgccagcc
    54001 atcacacgcg ccgccacttt tgccagcggt acgccggtgg ctttggagac gaacggaaca
    54061 gtacgcgccg cacgcgggtt tacttcaatc aggtaaactt cgttgttttt caccgcaaac
    54121 tgcacgttca tcagaccgcg cacctgcaat tcgaaggcca gtttctgcac ctgctggcgc
    54181 atcacatcct gaatttcctg acttaaggtg tacgctggca gcgaacatgc ggagtcaccg
    54241 gagtgcacac ccgcctgctc gatgtgctcc atgatgccgc caatcagcac catttcgccg
    54301 tcgcagatgg catccacgtc aacttctact gcgtcatcaa ggaaatgatc cagcaacact
    54361 ggcgcatcgt tagacacgct aaccgccgtc tggaagtagc gacgcaggtc agcttcgtca
    54421 tagacgattt ccatcgcccg accgccgaga acgtaagacg gacgcaccac cagcgggtag
    54481 ccaatctctt ttgccttctc aactgccatt tcaatagcag taacggtggc gttcgccggt
    54541 tgtttcagtt tcagacggtc aaccgcatgc tggaagcgtt cacggtcttc cgcacggtcg
    54601 atagcatccg ggctggtgcc gataaccggt acgccagcgg cttccagtgc acgcgccagt
    54661 ttcagcgggg tctgaccgcc gtactgaacg ataacgcctt tcggcttctc aatacgcacg
    54721 atttccagca catcttccag agttaccggc tcgaagtaga ggcgatcgga agtgtcgtag
    54781 tcggtagaga cggtttccgg gttacagtta accataatgg tttcgtaacc gtcttcacgc
    54841 agcgccagcg aggcgtgtac gcagcagtag tcgaattcga taccctgacc gatacggttt
    54901 ggaccgccgc caagcaccat gattttttca cggtcggtag acggattcgc ttcgcactct
    54961 tcttcataag tggagtacat gtaagcggtg tcggtggcaa actctgccgc acaggtatcc
    55021 acgcgcttgt agaccgggtg caggtcatat tggtcacgca gcttacggat ttccgcttcg
    55081 cgtacgcccg ccagttttgc caagcgcgca tcggcaaagc ctttgcgttt cagctggcgc
    55141 aggaattcag cgttcaggcc agtgatgccc acttccgcca ctttctcttc cagacgcacc
    55201 agttcttcaa tctgtaccag gaaccagcgg tcaatgttag tcaggttgaa gacgccgtcc
    55261 acagacaggc ccgcgcggaa agcatcggcg atgtaccaga tacgctctgc gcctgcgtct
    55321 ttcagttcgc gacggatttt ggttaacgct tccgggtcat ccaggctcac tttcgggtcg
    55381 aagccagttg cgcccacttc caggccgcgc agcgcttttt gcagggattc ctgctgcgtg
    55441 cgaccaatcg ccatcacttc gccaaccgat ttcatctgag tggtcagacg gtcgttagca
    55501 ccggcgaatt tttcgaagtt gaagcgagga attttagtaa ccacatagtc gatggacggc
    55561 tcgaaggagg ccggagtacg tccgccagtg atgtcgttca tcagttcgtc tagggtgtaa
    55621 cccaccgcca gtttcgccgc cactttagca atcgggaaac cggtcgcttt cgacgccagc
    55681 gccgaagaac gggacacgcg tgggttcatt tcgataacaa tcaggcgacc gtttttcggg
    55741 ttcaccgcaa actggacatt ggaaccaccg gtttcaacgc cgatttcacg cagcaccgcc
    55801 atcgaggcgt tacgcatgat ttgatattct ttgtcggtca gcgtttgggc tggcgcgaca
    55861 gtgatggagt caccggtgtg gatgcccatc gcatcaaagt tttcgataga gcagacgatg
    55921 atgcagttgt cgtttttatc acgcaccact tccatctcgt actctttcca gccgatcagc
    55981 gactcatcaa tcagcaactc tttggtcgga gagagagcca gaccgcgggc gcaaatttct
    56041 tcaaactctt cacggttata agcgataccg ccgccgctac cgcccatggt aaaggatggg
    56101 cgaataatgc acgggaagcc cacgtcagcg gcaacagcca gcgcttcttc catcgtgtgt
    56161 gcgataccgg aacgcgcggt ttccagacca attttcttca tcgctacgtc gaaacgacgg
    56221 cggtcttctg ctttatcaat cgcatcggca gtggcaccaa tcatggtgac accgaactct
    56281 tccaacacgc cctgacgttc cagctccagc gcgcagttca gcgccgtctg accgcccatg
    56341 gttggcagca ccgcgtccgg gcgctctttt tcaataatct tgcgtaccac ttcccagtga
    56401 atcggctcga tgtaggtcgc atcggccatt tccgggtcgg tcatgatggt cgccgggttg
    56461 gagttcacca gaatgacgcg gtaaccctct tcgcgcaggg ctttacatgc ttgcgcgcca
    56521 gagtagtcaa actcacacgc ctgaccgata acaatcgggc ccgcacccag aatcaggata
    56581 ctttttatat ctgtacgttt tggcatggct cttttactcc tgattactta gcggttttac
    56641 ggtactgctc aattaacttg ataaagtggt cgaacaacgg cgcggcgtcg tgtggaccag
    56701 ggctggcttc agggtgaccc tggaagctga acgccggctt atcgatgcga tgaatgccct
    56761 gtaacgtacc gtcgaacagg gatttatgcg tgacacgcag gtttgcaggt aatgttgctt
    56821 catccaccgc aaaaccgtgg ttctgggcgg tgatcatcac cacgtttttc tccacatctt
    56881 taaccggatg gttgccgccg tggtgaccaa atttcatttt gacagtcttc gcaccgctcg
    56941 ccagcgccag cagctgatga ccgagacaga tgccgaatac cggaatatcg gtttcgagga
    57001 acttctggat ggcggtaatg gcgtaatcgc acggggccgg gtcgccagga ccgttggaga
    57061 ggaagatgcc gtctggattc attttcagca catcttccgc agaagtttgc gccggaacga
    57121 tggtcaggcg acagcctcta tccaccagca tccgcaggat gttgcgcttg gcaccaaaat
    57181 cataagccac gacgtggaac ggcagctcgt cttctttttt cgcttctggc aggccgccgg
    57241 tcaacgtcca gctcccttgt gtccagctat aggcttctgc ggtggtcact tcttttgcca
    57301 gatccatgcc gttcagacct gggaacgcgc gggctttttc taacgccagc gccgcatccg
    57361 ggttatcgcc tgcgataatg cagccattct gtgcgccttt ctcgcgcagt aaacgcgtca
    57421 gcttacgggt atcgatatcg gcaatcgcca cgatgttatg gcgcttcagg taagaagaga
    57481 ggtcttcggt attacggaag ttgctggcaa tcagcggcag gtcgcgaatc accagacctt
    57541 gtgcatgtac ctgagaagat tcttcatcgg cgtcattagt gccgacattg ccaatatggg
    57601 gataagtaag agtaacgatt tgacgagaat aggaaggatc agtgaggatt tcttgataac
    57661 cggtcattga agtattgaaa acgacttccc caaccgccga acctgttgcc cctatggccc
    57721 gaccgtgaaa ctgggttccg tcttccagaa ccaatagcgc tgacttaatc aaaacaccct
    57781 ccagagaata ctcactcact ttatttgcat attaattcac agtgatgacg taaatcaatg
    57841 caaatctgct taccgacgaa tttctggcaa acggcggcat tctggagata tgggcgctaa
    57901 aagtcaactt aatggcagga ttttttatat tattttgtgg ctcatgcatg ttttacaggc
    57961 attaagcgac aaaaacatca tctaacctgt tctggaaaac gcttgcgcag caagaagatt
    58021 gcatgaaata aaaacgagca ggaaaaagtg gaccaaatgg tcaaaattta cattaaaaca
    58081 acaaaaatag aagccgaaac atgtaatttt tggcagttat agaaaattta aaagcataac
    58141 accaaaaata aaagggctaa aaattaaagc cctttataat taataaatta aatgtgttac
    58201 ttttgcatca taataaacat tttgtggtta caaattattg agatcaagca catctcgcat
    58261 atcaaaaaga ccactttcct taccactcaa ccacaaagcc gatcttaccg caccgttagc
    58321 aaatgtcatg cggctggacg ccttatgggt gatctccaga cgctcgccaa tatcggcaaa
    58381 catcgcggta tgttcgccaa cgatgtcacc tgcacgcacg gtagcaaaac caatggtacc
    58441 tggcacacgt tcaccggtat ggccttcacg actgtagacc gcgcaatctt tcagatcttt
    58501 atcaagggcg tgggcgatcg cctctcccat tgccagtgcg gtgcctgacg gcgcatcaac
    58561 tttatgtcta tgatgtgctt caataatttc gatatcggtg tagtcaccca tcactttggc
    58621 tgctttctcc agcagcttaa gcatgacgtt aacgccaacg ctaaaattgg cggcaaagac
    58681 aatcgcaata tcggcggcgg cgtcacgaat tgcctgttta ccggcttcgt caaaccccgt
    58741 agtgccgatc accatccctt tgccatgctg ccgacaaaaa gcgagatggt tcagcgtacc
    58801 ttccggacgg gtaaaatcga taaacacatc aaaatcatct tttatcgcat caagactgct
    58861 ttgcacggta acgcctgttt tccctgcacc agccagctca ccggcgtcgc tgcccagtaa
    58921 agaagatcct tcgcgctcca gcgccgcgcc cagctgcacg ccttctaatg ccagtgccgc
    58981 ctgaatcaac tggcggccca tacgcccccc ggctcccgcg atggcaacgc ggatgtttgc
    59041 atcatgcata gctattctct tttgttaatt tgcatagacc gttttcagag taaccagcgc
    59101 atcgctacgc cgccagccaa aaacgctgat aattagaaat taatgataaa cagggaggga
    59161 tatcagtgaa aggcatgagt cgatgaatga ctgcatgccg ataacatgtg acaggttacg
    59221 gcaccagagc cagcacctca gccacccact gttggaagcc ttttacattc agatccaatg
    59281 ccacctgtac attggctggt ttgcccaggc aaccgtcgat atcaaccacc gtcgtgcctg
    59341 aggtaaattc gccttgagtt tctaccgcga caaaacacgg cttgagggtg aataagtccg
    59401 ggcgcaccag ccaggcgatg gcgcagagat cgtgcattcg cagaccgctt tgcatactgc
    59461 cgctacggta gtggctaaac agggcgtgga gcatttttcc ggtgcgattc aactccggta
    59521 aagtagcgag atagtcaggg gttaatattg cctgattggt gacatccaga ccgcacatga
    59581 caatttcgat gccgctacgg aagacacagg cagccgcttc tggatcggca gcgatattaa
    59641 actcggcatt tggtgtacag ttgccgcgac cagcagaacc gcccatgatc accagacgac
    59701 gaatatacgg tttacattcc gggcattgta aaagtaacag cgcaatattg gttaacgggc
    59761 cgatggccac cagggtaacg ggctctggtg cacgcatcag ggcatcccga atcgccagaa
    59821 acgccggtat cccgagtggc tttcggttgt gctcaacaaa gtcgtagcca gccattcccg
    59881 attcgccgtg cacagatgct gcatcacgcg gtgcgcgtac cagcggcaca gcggctccct
    59941 gggcgagcgg aatctccgca ttccagaaat gcagcagttg cagggcattg cgggtagttt
    60001 tctcaaccga gacattaccc gcgacggtgg tcatcagttg caggtcgagt tcgggggcaa
    60061 aaatcgcggc ggcaatggcg acggcatcgt caatgccggg gtcagtatcg aggaagatag
    60121 gtaaacgcat gttttcttca taaaaaatgc cggtaacaag accggcattt tcgcataact
    60181 taggctgcta atgacttaat cgacttcacg aatatcgaca cgcagctctt tcggcacttc
    60241 gaaaacaata ttttcttcac ggccttccag cggaatggct tcaccaccac ccaactgctg
    60301 caaacgtgcc accacattct gcaccagaat atccggagcc gatgcgcccg cagtcacgcc
    60361 gacgcattta acctctttca cccactcttc ctggatatct ttcgcatcgt caatcaaaaa
    60421 cgcgtgtttg cccatacgct gggccagctc cgccagacgg ttggagttgg aggagttttt
    60481 cgaaccgacc accaacacaa cttccgcctg ttctgccagg gcgcgtaccg cttcctgacg
    60541 gttagtcgtg gcgtagcaga tgtcatcttt gcgcggaccg acaattttcg ggaagcgttt
    60601 acgcagcgcg tcgatcacat cagacgtgtc atccaccgac agcgtggttt gggtcataaa
    60661 ggagagcttc tcttcgtttt tgaccgtcag tttccacaca tcgtctggcg attcgaccag
    60721 atacattccc ccttcggggt tgctgtactg ccccatcgtc ccttccactt ccgggtgccc
    60781 ggcgtggccg atgagaatag attcttcgcc acggcgactg gcgcgggcaa cttccatatg
    60841 cactttggtc accagcggac aggtggcatc aaacaccgtc aaatcgcggc ttttcgcttc
    60901 gttacgaacc gcctgagaaa caccgtgtgc ggagaaaatc aggatcgcgc cgtccggcac
    60961 ttcgctaatc tgctcaataa agatagcccc acgctcgcgc aagctatcga ccacgtagcg
    61021 gttatgcacc acttcgtgac ggacatatat cggtgcgccg taaatggcca gcgcgttttc
    61081 aacaatgctg atagcgcggt ctaccccggc acaaaaacca cgcgggttgg ccaacaggat
    61141 ctgcatgtta cgcctccagt gccggatcga tttccagcac ttcaatatca aaatgaacgg
    61201 tctgcccggc cagcggatgg ttgaaatcaa cggtaatgga gtcgccgtta atttcgcgga
    61261 tcacaccagg catctcactg ccatccattg cggtaaaaag catgattgcg ccaatttctg
    61321 gctcgcctgc atccataaat tcacggcggg agaagtactg aatcaggtcc ggtgacggca
    61381 cgccaaacgc cgcatctggc tccagcgaga aggtggtttt gtcgcccact ttcagcccca
    61441 gcaggtgttg ctccagccct tcagaaagag aagcatcgcc caggcggaac agcgccggtt
    61501 taccgttgtt gcgagtagac tcggcggtgg tgccatcgtc gagttttagc gtgaagtgca
    61561 ccaggacggc gctattgctc tgtacagatt cagacatgca ggtttgctct tatttttgag
    61621 gatttttgcc ggatgcggcc tacgatttcg tagcaatctg taggccgcat ccggcagggt
    61681 ttattattgt tttttcgctt tggaaggtaa aaacccttcc agcacgatca gtgccgcacc
    61741 gacacaaatg gcagtatcgg caaggttgaa ggtggcgaag tgccagtcgc cgacgtagaa
    61801 gtcgatcata tcgacaacga agccgtgcca caggcggtcg aacaggttgc ccagcgcgcc
    61861 gccaataatc agcgcgtaag cgatattgtt tagcttctgc gtggccttcg agcgatacat
    61921 catcactgcc aggagcacgc taataccaat cgcaataccg gcaaagaacc aacgctgcca
    61981 gccgccgcta tcggcaagga aactaaacgc cgcgccatag ttacgcgcat aatgcagatt
    62041 aagcgacggg aacagcggga ccgtatcccc cagagcaaag ttctggagga tcaggtattt
    62101 gctgcccaga tcgataatca gcacgactac caccagccac agccagcgta gccctgttga
    62161 acagatcgat tgactcatca ggcaaactta cgtttttcac cgtcaccggc gacgttgctg
    62221 acacagcggc cgcagatttc tgcgtgttcc gccaccttgc cgacatcctg ggtgtagtgc
    62281 cagcagcgtg ggcacttctc accttcggct ttactcaacg cgactttcag ccctttgagt
    62341 acttcgctct gctgagcatc agcaggtgcg tcgttatagt ctgcaacggt agcgccggag
    62401 gtcaacagga caaatcgtaa ttcatcgccc agcgcggtca gtttcgccgc cagttccggt
    62461 tctgcataca aggttactgc cgcttccagc gagccaccca ctttcttgtc ggcacgcgct
    62521 tgctcaatga ctttgttcac ttcgccacgc actttcaaca gctcgtccca gaacgcatcg
    62581 ttcattgctt cactgtctgc cagaccaaac aggccttcgt accactcacc ggtgaagacg
    62641 tatttttcac gttcgcccgg cagatagccc cacacttcat cagcggtgaa ggagaggatt
    62701 ggtgccatcc agcgcaccag cgcttcagcg atgtgataca gcgcagtctg gcagctacga
    62761 cgcgccacgc tatccgcttt ggcggtgtac tgacggtctt tgatgatgtc gaggtagaag
    62821 gaacccatct caacggagca gaagcgcatc agacgctgta ccacttcgtg gaaatcgtat
    62881 gcttcgtacg ccttgaggat gtcttcctgt gccgctttcg cacaacctac ggcccagcga
    62941 tccagtacca ccatctcttc cggtttcacc atatcttttg ctggatcaaa accattcagg
    63001 tttgccagca ggaagcgcgc ggtgttacgg atacgacgat agctatcggc agcacgtttc
    63061 aggatctcgt cagaaacggc catttcaccg gtgtagtcgg ttgatgccac ccacagacgc
    63121 agaatatccg cgcccagttt gttcatcaca tcctgcggcg aaacggtgtt gccgatggat
    63181 ttagacatct tgcgaccctg accatccacg gtaaagccgt gggtcagtac ctgacgatac
    63241 ggcgctttgc ctttcatcgc ggtggagatc attagggaag acatgaacca accacggtgc
    63301 tggtcagaac cttccagata catgtccgct gcgtgaccgg caaattccgg acgcacgtca
    63361 acaacagaag agtgggtaga tccggagtca aaccatacgt cgagagtatc cggcactttc
    63421 acatactggt cagcttcgtc gccgaggatc tctttcgcat cgagatccca ccacgcctgg
    63481 atgccgtcaa cttcaacgcg tttagccact tcttccatca gttcgagggt acgcggatgc
    63541 agctcttccg tgtctttgtg cacgaacagt gacatcggta cgccccaggt gcgctgacgg
    63601 gagatacacc agtcaggacg gttagcaacc atcgactcga tacgcgcctg gccccagtcc
    63661 gggagccact gcacgccttt gatctctttc agtgactgcg cacgcagacc tttctgatcc
    63721 atgctgacaa accactgcgg tgtcgcgcgg aagatgatcg gcgttttgtg acgccagcag
    63781 cacggatagc tgtgctgcat tttctcaacg tgcagcagcg cgcctttttc ctgcagcagc
    63841 gcaacgacga tgtcgttcgc tttgaagacg ttcacgccat ccagcgtcgg ataagtgccc
    63901 ggcagataag tgccgtccgg gccaaccggg ttagcggttt ccaggccgta tttctgaccg
    63961 atcacatagt cgtccgggcc gtggccaggc gcggtgtgaa cggcaccggt accggcatcc
    64021 agggtaacgt gatcgccgag gattgccgga acgtcgaagc ccataaacgg atgggtaaag
    64081 cgcagcagct caagctctgc accttttacc gtgccgagaa tggtgtaatc ggtcacgccg
    64141 atacgctgca ttacgctttc aaccagatct ttcgccagaa tcacggcctg accgtcgatc
    64201 tgcaccagcg catagtcgaa atcaggtgca atagagattg cgcggttcgc aggcagagtc
    64261 cacggcgtag tggtccagat caccagcgag attgggccgt taacgttgct tacgccaaat
    64321 ttcgctttca gcgcatcctg atcgaccgcc tggaaagcga cgtcaatgga cggagaagtt
    64381 ttgtcgtaat actcaacttc cgcttctgcc agtgcagaac ggcagtctac gcaccagtgc
    64441 accggcttcg cgcctttgtg caggtgacca ttgccgatga ttttgcccag cgcgcggatg
    64501 atgttggctt cagttttgaa gtccatagtc agatacgggt gcgaccagtc gcccagcacg
    64561 cccagacgga taaagtcttt gcgttgaccg tcaacctggg tcgccgcgta ttcgcggcac
    64621 ttggcgcgga actcggcggc ggtgaatttc tcacccggct taccgtattc ttgctctact
    64681 ttcagctcga tcggcagacc gtggcagtcc cagccaggca catacggcga gtcataaccg
    64741 gaaagccctt tggacttcac gataatgtct ttcagaatct tgttaaccga gtgaccaata
    64801 tgaatgctgc cattcgcata aggagggcca tcatgcagaa tgaaggtttt tttgcctttt
    64861 ttagccgcac ggatgatgcc gtacagatca tcatcagtcc aacgcgccag cattcccggt
    64921 tcgcgcttgg cgagatcgcc acgcatcggg aaccctgttt ccggcaaatt cagggttgat
    64981 ttatagtcac tcatcagatt ctcggttccg tgtttcggtt tgattacata acaggcttaa
    65041 gccggttttg ttagcccaaa aaattcgcgg gcggttaatt catcacgcgc aatctgcgct
    65101 ttcagttcgt ccagcgacgc aaatcgctgc tcattgcgta tttttttacg cagcactact
    65161 tgtatatggc gaccgtaaag gtccattgca acatctaaca aatgcacttc cagctgctgg
    65221 cgaataccgg caaccgttgg gcgtgttccg atgtttgcca cgccgggtaa cggcttttca
    65281 ccgaggccca gcacttctac cgcataaacc cctttcaccg gggaaacctg acggcgtagc
    65341 ggtacattcg ccgtcgggaa acctatagtg cgccctaatt catcaccgtg gactacacgc
    65401 ccggagatgg caaacgggtg ccccagtaaa ctttctgcca gagccagatt gtcatcagcc
    65461 agcgcctgac gcacggcggt gctgctgata cgcacaccac cttcgcaaaa agtttgcgta
    65521 ctggtgatat cgaagccgta ttccatgcca gctttctgta ataacaagaa atcgccttca
    65581 cgaccagcgc caaagcggaa atcatcacct acggcaagaa attttacgcg caaatgcttc
    65641 accaaaagat cgctgatgaa attttgcgcg gttaacgccg caaaacgcct gtcgaaacgc
    65701 acgcacagca cgtaatcaac gccacactct gcaaggtaac gcagtttttc ccgcagccgg
    65761 gtcagtcttg ccggggcttt atcggtagca aacagttcca gtggttgagg ttcaaaaagc
    65821 atcaccatca ccggtaagtt gcgcttgcgc ccttcttcct gcaagccctg taacagcgcg
    65881 cgatgaccgc gatgcacgcc gtcgaaatta ccaatagtca gcacacaccc ttcttgcggg
    65941 gcctggctga gattatgtat gccgcgtatc agcttcatgt ctggctcaaa acagtgaaaa
    66001 tcgtccgagt ataccttgta cagcggtcaa ggttaaccgg cgattgagta ccgaaatcag
    66061 aaagaagtgg cttttcattg ccatggagca aatcacggga agaaactgac cgcctgctgc
    66121 aatttttatc gcggaaaagc tgtattcaca ccccgcaagc tggtagaatc ctgcgccatc
    66181 actacgtaac gagtgccggc acattaacgg cgcttatttg cacaaatcca ttgacaaaag
    66241 aaggctaaaa gggcatattc ctcggccttt gaattgtcca tatagaacac atttgggagt
    66301 tggaccttgg ctaatatcaa atcagctaag aagcgcgcca ttcagtctga aaaggctcgt
    66361 aagcacaacg caagccgtcg ctctatgatg cgtactttca tcaagaaagt atacgcagct
    66421 atcgaagctg gcgacaaagc tgctgcacag aaagcattta acgaaatgca accgatcgtg
    66481 gaccgtcagg ctgctaaagg tctgatccac aaaaacaaag ctgcacgtca taaggctaac
    66541 ctgactgcac agatcaacaa actggcttaa tcgccaattt gctgaagctt tgtgaaaaag
    66601 cccgcgcaag cgggtttttt tatgcctgct gcttttgtga tgcgtgagag aacacactgc
    66661 cggttgccag atagtcaaaa gagacatgcc gttgtactat ctgaactctt cgtggcttaa
    66721 gaatcgtaga accatcggct gatacattct gaacaagaat atctactcaa tctccagact
    66781 cccccacaaa gaatatggat attgtgatac acattgaggt attaaactta agaccactct
    66841 atttttgaat taaattccta tatactttaa ccattcataa atatctcctc attagacct
//
LOCUS       DAOFMX010000009        65507 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.009, whole genome shotgun sequence.
ACCESSION   DAOFMX010000009 DAOFMX010000000
VERSION     DAOFMX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.009"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            131..574
                     /locus_tag="QBA20_000715"
     CDS             131..574
                     /locus_tag="QBA20_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ6291365.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="QBA20_000716"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="QBA20_000716"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDQ6291366.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="QBA20_000717"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="QBA20_000717"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDQ6291367.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="QBA20_000718"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="QBA20_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDQ6291368.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="QBA20_000719"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="QBA20_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6291369.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="QBA20_000720"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="QBA20_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDQ6291370.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="QBA20_000721"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="QBA20_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDQ6291371.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="QBA20_000722"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="QBA20_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDQ6291372.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTM
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVLHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="QBA20_000723"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="QBA20_000723"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDQ6291373.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="QBA20_000724"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="QBA20_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDQ6291374.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="QBA20_000725"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="QBA20_000725"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDQ6291375.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVVEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="QBA20_000726"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="QBA20_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDQ6291376.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="QBA20_000727"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="QBA20_000727"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDQ6291377.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="QBA20_000728"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="QBA20_000728"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDQ6291378.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="QBA20_000729"
     CDS             13934..14314
                     /locus_tag="QBA20_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDQ6291379.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="QBA20_000730"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="QBA20_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDQ6291380.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="QBA20_000731"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="QBA20_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDQ6291381.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="QBA20_000732"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="QBA20_000732"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDQ6291382.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="QBA20_000733"
     tRNA            complement(17415..17490)
                     /locus_tag="QBA20_000733"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="QBA20_000734"
     tRNA            complement(17494..17569)
                     /locus_tag="QBA20_000734"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="QBA20_000735"
     tRNA            complement(17621..17696)
                     /locus_tag="QBA20_000735"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
     gene            complement(17699..17774)
                     /locus_tag="QBA20_000736"
     tRNA            complement(17699..17774)
                     /locus_tag="QBA20_000736"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17739..17741),aa:Val,seq:tac)
     gene            complement(17911..17986)
                     /locus_tag="QBA20_000737"
     tRNA            complement(17911..17986)
                     /locus_tag="QBA20_000737"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17951..17953),aa:Lys,seq:ttt)
     gene            complement(18151..18942)
                     /gene="cpoB"
                     /locus_tag="QBA20_000738"
     CDS             complement(18151..18942)
                     /gene="cpoB"
                     /locus_tag="QBA20_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDQ6291383.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18952..19473)
                     /gene="pal"
                     /locus_tag="QBA20_000739"
     CDS             complement(18952..19473)
                     /gene="pal"
                     /locus_tag="QBA20_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDQ6291384.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19508..20800)
                     /gene="tolB"
                     /locus_tag="QBA20_000740"
     CDS             complement(19508..20800)
                     /gene="tolB"
                     /locus_tag="QBA20_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDQ6291385.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20933..22207)
                     /gene="tolA"
                     /locus_tag="QBA20_000741"
     CDS             complement(20933..22207)
                     /gene="tolA"
                     /locus_tag="QBA20_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDQ6291386.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADK
                     KAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNG
                     ASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQ
                     AALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22272..22700)
                     /gene="tolR"
                     /locus_tag="QBA20_000742"
     CDS             complement(22272..22700)
                     /gene="tolR"
                     /locus_tag="QBA20_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDQ6291387.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22704..23396)
                     /gene="tolQ"
                     /locus_tag="QBA20_000743"
     CDS             complement(22704..23396)
                     /gene="tolQ"
                     /locus_tag="QBA20_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDQ6291388.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23393..23797)
                     /gene="ybgC"
                     /locus_tag="QBA20_000744"
     CDS             complement(23393..23797)
                     /gene="ybgC"
                     /locus_tag="QBA20_000744"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDQ6291389.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23947..24240)
                     /gene="ybgE"
                     /locus_tag="QBA20_000745"
     CDS             complement(23947..24240)
                     /gene="ybgE"
                     /locus_tag="QBA20_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDQ6291390.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24240..24353)
                     /gene="cydX"
                     /locus_tag="QBA20_000746"
     CDS             complement(24240..24353)
                     /gene="cydX"
                     /locus_tag="QBA20_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDQ6291391.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24368..25507)
                     /gene="cydB"
                     /locus_tag="QBA20_000747"
     CDS             complement(24368..25507)
                     /gene="cydB"
                     /locus_tag="QBA20_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDQ6291392.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25523..27091)
                     /gene="cydA"
                     /locus_tag="QBA20_000748"
     CDS             complement(25523..27091)
                     /gene="cydA"
                     /locus_tag="QBA20_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDQ6291393.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27940..30573)
                     /gene="mngB"
                     /locus_tag="QBA20_000749"
     CDS             complement(27940..30573)
                     /gene="mngB"
                     /locus_tag="QBA20_000749"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDQ6291394.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30591..32507)
                     /gene="mngA"
                     /locus_tag="QBA20_000750"
     CDS             complement(30591..32507)
                     /gene="mngA"
                     /locus_tag="QBA20_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDQ6291395.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32677..33399
                     /gene="mngR"
                     /locus_tag="QBA20_000751"
     CDS             32677..33399
                     /gene="mngR"
                     /locus_tag="QBA20_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HDQ6291396.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNVFNTDDYKFTLIAQRKSSR"
     gene            complement(33502..34371)
                     /gene="sucD"
                     /locus_tag="QBA20_000752"
     CDS             complement(33502..34371)
                     /gene="sucD"
                     /locus_tag="QBA20_000752"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDQ6291397.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34371..35537)
                     /gene="sucC"
                     /locus_tag="QBA20_000753"
     CDS             complement(34371..35537)
                     /gene="sucC"
                     /locus_tag="QBA20_000753"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDQ6291398.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35631..36848)
                     /gene="odhB"
                     /locus_tag="QBA20_000754"
     CDS             complement(35631..36848)
                     /gene="odhB"
                     /locus_tag="QBA20_000754"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDQ6291399.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36863..39664)
                     /gene="sucA"
                     /locus_tag="QBA20_000755"
     CDS             complement(36863..39664)
                     /gene="sucA"
                     /locus_tag="QBA20_000755"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDQ6291400.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39965..40681)
                     /gene="sdhB"
                     /locus_tag="QBA20_000756"
     CDS             complement(39965..40681)
                     /gene="sdhB"
                     /locus_tag="QBA20_000756"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDQ6291401.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40697..42463)
                     /gene="sdhA"
                     /locus_tag="QBA20_000757"
     CDS             complement(40697..42463)
                     /gene="sdhA"
                     /locus_tag="QBA20_000757"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDQ6291402.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42463..42810)
                     /gene="sdhD"
                     /locus_tag="QBA20_000758"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42463..42810)
                     /gene="sdhD"
                     /locus_tag="QBA20_000758"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDQ6291403.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42804..43208)
                     /gene="sdhC"
                     /locus_tag="QBA20_000759"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42804..43208)
                     /gene="sdhC"
                     /locus_tag="QBA20_000759"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDQ6291404.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43589..43696
                     /locus_tag="QBA20_000760"
     CDS             43589..43696
                     /locus_tag="QBA20_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291405.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43902..45185
                     /gene="gltA"
                     /locus_tag="QBA20_000761"
     CDS             43902..45185
                     /gene="gltA"
                     /locus_tag="QBA20_000761"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDQ6291406.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45576..46142
                     /locus_tag="QBA20_000762"
     CDS             45576..46142
                     /locus_tag="QBA20_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6291407.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46193..48652
                     /locus_tag="QBA20_000763"
     CDS             46193..48652
                     /locus_tag="QBA20_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ6291408.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSGSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48667..49398
                     /locus_tag="QBA20_000764"
     CDS             48667..49398
                     /locus_tag="QBA20_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDQ6291409.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49395..50471
                     /locus_tag="QBA20_000765"
                     /pseudo
     CDS             49395..50471
                     /locus_tag="QBA20_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50623..51669
                     /locus_tag="QBA20_000766"
     CDS             50623..51669
                     /locus_tag="QBA20_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDQ6291410.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51666..52457)
                     /gene="nei"
                     /locus_tag="QBA20_000767"
     CDS             complement(51666..52457)
                     /gene="nei"
                     /locus_tag="QBA20_000767"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDQ6291411.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKTKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52493..53227)
                     /gene="pxpA"
                     /locus_tag="QBA20_000768"
     CDS             complement(52493..53227)
                     /gene="pxpA"
                     /locus_tag="QBA20_000768"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDQ6291412.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53217..54149)
                     /gene="pxpC"
                     /locus_tag="QBA20_000769"
                     /pseudo
     CDS             complement(53217..54149)
                     /gene="pxpC"
                     /locus_tag="QBA20_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54143..54799)
                     /gene="pxpB"
                     /locus_tag="QBA20_000770"
     CDS             complement(54143..54799)
                     /gene="pxpB"
                     /locus_tag="QBA20_000770"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDQ6291413.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54822..55565)
                     /gene="ybgI"
                     /locus_tag="QBA20_000771"
     CDS             complement(54822..55565)
                     /gene="ybgI"
                     /locus_tag="QBA20_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDQ6291414.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55836..57317
                     /gene="dtpD"
                     /locus_tag="QBA20_000772"
     CDS             55836..57317
                     /gene="dtpD"
                     /locus_tag="QBA20_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDQ6291415.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRSVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57467..58885)
                     /gene="phrB"
                     /locus_tag="QBA20_000773"
     CDS             complement(57467..58885)
                     /gene="phrB"
                     /locus_tag="QBA20_000773"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDQ6291416.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58882..59391)
                     /locus_tag="QBA20_000774"
     CDS             complement(58882..59391)
                     /locus_tag="QBA20_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDQ6291417.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59520..59807
                     /locus_tag="QBA20_000775"
     CDS             59520..59807
                     /locus_tag="QBA20_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HDQ6291418.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59862..60242)
                     /locus_tag="QBA20_000776"
     CDS             complement(59862..60242)
                     /locus_tag="QBA20_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291419.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60245..64909)
                     /locus_tag="QBA20_000777"
     CDS             complement(60245..64909)
                     /locus_tag="QBA20_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDQ6291420.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPAST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETVFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATVAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLAVTDPLQQNTRYQYDHHGQVVQ
                     ITDARGGNKYLHWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAQSHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDAMGRRTAF
                     GYDAYGRLVTLTNENGERYRFRHDVLDRLAEQINPDGCRQSYRYNALNAVTEVVFTGD
                     RGGEIRHRLARDAAGRLTAKETADGRTEYVHDAADQLLEIRRQRHETDAPEVIRFSYD
                     RLGRMLSEETAQGILTHQYDKLGNRTATTFPDGRTQRHLYYGSGHLQQINLDREVISE
                     FTRDHLHREVLRSQGRLSTRQLYDPGGRLKRRETYSGMRGVVPETFTDRQYSYSGEDE
                     LLKTRHSRRGETDYFYDPTGRITACRSEDEGYLASWQYDAAGNLLGRRAGERATAENS
                     VVPFNRLMSYRGVHYRYDEFGRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYD
                     ALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVD
                     KAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRY
                     QGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTP
                     CAVSNQKANRLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYL
                     TGNKTKAGSYHWDDIKDPSVTKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPW
                     ET"
     gene            complement(64928..65131)
                     /locus_tag="QBA20_000778"
                     /pseudo
     CDS             complement(64928..65131)
                     /locus_tag="QBA20_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15878 a        17742 c        15991 g        15896 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc aggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactatggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtctgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgttttgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccacaagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctaaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatctcg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gcgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ttgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt acattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
    17761 tgagctaatc acccgacact acgctggata ctacaactaa ctatgttagt ggtgggtcgt
    17821 gcaggatgac tcgacttcgt ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc
    17881 acgttttact accgcttcca ccttagcgat tggtgggtcg tgcaggattc gaacctgcga
    17941 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccactt tttcgttgct
    18001 ttcggtttgt ttgatatccc gtggcaacgg cggcatatat tactgatttc agacttgagc
    18061 gcaacaaaaa tttcgatgca gatcactcaa ctgcttatga ttcgcacgac acgaccagaa
    18121 ataatgcgac ttctggtcgt gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg
    18181 tttagcgcca tcagtaccag ggtatttact gataacctgc tggtatacgg ctttcgcttt
    18241 tgcggtgtca cctttgtcct gcatgatgac gccgacttta aacatcgcat cagcagcctt
    18301 tggtgacttc ggatagtttt tcactaccga agcaaaatag tacgccgcat catctttttt
    18361 acccttgttg tagtttaact gacccagcca ataattggcg tttggcaggt aagttgaatc
    18421 agggtaattt ttgatgaaat tctgaaatgc caccattgcg tcatcctggc gggatttatc
    18481 ctgcaccagc gcaatagctg cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc
    18541 gccagcattc gcagtaccag catcagctgt cggcgtcgtt gatgccgtcg caccgctttg
    18601 atcgctgctg gttgattgcg ccgctgcacc accgctgctg aggctgtcga tctgcaacag
    18661 gatctgcttt tgccgctcca cgacctgatt cagttgatac tgattttcct gaatctgacc
    18721 acgcagggaa tcaatatcgg attgattatc agaaagttgt tgctggagtt gggttaaaag
    18781 ctggctgtga gcattagaaa tacgctcaag ttgagtgacg cggtcttcga ccgagcctga
    18841 gccgacacta ctgattggtg cctgagcaaa agcggcccag ggggccgcta taccaaccag
    18901 taacgacaga ctcaatagtt gatgtctgaa gttactgctc atgcaattct cttagtaaac
    18961 cagtaccgca cgacggtttt tggagtatgc cgcttcgtca tgacccagta ctgcaggttt
    19021 ttctttaccg taagaaacga tggagatctg gtctgcagaa acgcctttac cctgcaggta
    19081 catcttaacg gcgttcgcac gacgttcacc cagggagatg ttgtattccg gagtaccacg
    19141 ttcgtccgcg tgaccttcta cggtgacttt gtaagacggg ttgctacgca ggaagtttgc
    19201 atgtgcatcc agcatttgag cgaagtcaga acggatatcg tacttgtcca gatcgaagta
    19261 aacgatgttg ttctgctgca gctgttgcat ttgcagacga gcctgctctt cggaagacat
    19321 gttgccgttg ccgccgttcg catccatacc agtgccggca cccagcatgc cttcgctgcc
    19381 gtcattgctg gcgttcttgt tggaagaaca tgccgcaatt gccataacag gcagagcaat
    19441 catcagccct ttcagcactt tgttcagttg catttcaatg attcctttac tattcaatta
    19501 attattatca cagatacggc gaccaggcag ggaatttgac ctgtccatca gttgccggaa
    19561 gacgcgcttt gaaacgccca tctgtagaaa ccaaattcag cacggatccc atcccctgag
    19621 aagagctgta gattaccata gtgccgttag gtgccagact tggcgtttca tccaggaacg
    19681 tggacgacag aacttgtacg cctcccgttg ccagatcttg tttggcaatg tgctgctgcc
    19741 caccattgga gctgaccatt accataaatt taccgtcgct gctgacatcc gcatcttggt
    19801 tctgcgaacc ttcccaggta atacgttgtg gcgcaccgcc gttgatattc actttataaa
    19861 cctgcggacg accggcctgg tcagaagtaa atgccaggtt ctggctatcc gggaaccagg
    19921 tcggttcggt attgttactg cgaccatcag tcacctggcg gatctgacca gaagccaaat
    19981 ccattacgta caggttcaga ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt
    20041 ctggcgagaa tgcaggtgca ccgttgtgac gcgggaatga agccacctga cgtacagcgc
    20101 catttgccag cgtctgaata accagcgcgg aacgaccgct ttcgaaggtc acataagcca
    20161 gtttagaacc gtctggtgac cacgccggag acatcagcgg ctgcggtgaa cggtgaacga
    20221 caaactggtt gtaaccgtca tagtcagata cgcgcagttc atacgggaac tgaccgccgt
    20281 tggtctgaac aacgtaggca atacgggtac ggaacgcacc tttaatgccg gtcagttttt
    20341 caaacacttc atcactggcg gtatgaccag cataacgcag ccactgcttg ttcactttgt
    20401 acgagttctg agcaagtaca gtacccggtg cgccgccagt gtcaacaagt tgataagcaa
    20461 cattgtaaga accatccgga ttcggagtga cctgaccgac aactacagcg tcaatgccca
    20521 gtgcggacca tgcagctggt tgtacttcct gcgcactacc cggctgctgt ggcagacgag
    20581 cgcgatctaa cggattaaat ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa
    20641 tatcttcagg tgccgcacca ggccccgccc actggaaagg aacaacacca ataggacgac
    20701 cggaatctac accgctgtcg atcacaatgc ggacttcagc atgcagaact gatgcccaca
    20761 gtatgagaaa accaaatgct actcgtaatg cctgcttcat catatctccc ttatctggac
    20821 cgatggccca cgataattta gcagaatgtt aacaaactaa aatacacaaa actaccagaa
    20881 ccccgtggca accggtgcct gatgttgacc gtccgaacag tcaacatcgc gattacggtt
    20941 tgaagtccaa tggcgcgttt ttgaacactt catatactgc ctggcttggt ggtttcggga
    21001 tcttcgcaag tttagctgct gccaacgcag cctgacaaag tgcgggatcg ccaccttcag
    21061 gtttgatatc cagtaacata ccatcgggtg ccagttttat gcgcagcgta caggttttgc
    21121 ctgcatagga cgatgcgtca tagaacttac tttcgatagc agatttaatc tgcccggcat
    21181 agttattgat atcggcccct gatgcgccat tgtttttagt attaccactc ccggcaggcg
    21241 aagcattgtt ccctttcgcc cctccccccg ttttcggtgc attcttacca gagcttagct
    21301 caccgaaaat atcatctgcc tctgcggcag cttttgctgc agcggctttt tcagctgctg
    21361 cttttgccgc tgccgctttt ttgtctgcgg cggctttttc tgccgctgct ttcttatcag
    21421 ctgctgcctt atcagcagcc gctttttctg ctgctttttt gtcggctgca gctttctctg
    21481 ctgccgcttt cttatcagct gcagctttct ctgctgccgc tttcttatca gctgcagcct
    21541 tttcagcagc cgctttcttc tcagcttctg ccttggcttt ttcagcagct tcagttgccg
    21601 ctttctttct tgcttcagct gcagctgctt ctgccgcttc cgctttcttc ttcagtgccg
    21661 cagcggctac ctcggctttt ttctgcgctt cggctgcggc tttggcggct tctgcttctg
    21721 cttttttctt cgcgtctgca gccgctttct tcgctgcttc ttccgcagct ttagcatctg
    21781 ccgccgcttt cgctgccgcc acttcagctt gcttctgctt taactcggcc tgttttgcgg
    21841 cttcttcagc ctgctttttc tgctcctgag ccgctaaccg ctctttctca agttgcttca
    21901 ggcgttcctg ttcagccgct tgtttctcac gcagttcttc agcagcctgc tgttccttca
    21961 tcttgcgctg ttcatcagaa cgcttcgcgc ttgattcctg gctttgcatg cgtttgtact
    22021 gctcaactac cgcacctgaa tcaaccatga cagcgtcgat ggacgaacca ccgccgcctc
    22081 cggctgaagc ttctatattc tcatcgaacg aactccagat cagcgccgca aataagatga
    22141 catgcagcac tgctgaaata attatcgccc gcttgagctt gtcgttttgt tcggttgcct
    22201 ttgacactct cggtttccaa aaactgttcg cctgttaccc gctctctttc aagcaaggga
    22261 aacgcagatg tttagatagg ctgcgtcatt aaaccaaccg atttcacacc cgcactatgt
    22321 aacaagttca gtgctttaat tatttcatcg taaggcacat cttttgcgcc accgatcaga
    22381 aagaccgttt tcgggttggc cttgaaacgg ctggacactt ccgccaccac ctgctctggt
    22441 ggtaaacgct ccaggcgatc tttctcaacc accacggtgt actgaccaat accagacact
    22501 tcaacaatca ctggcggatt atcgttactg ctcaccgcct gtgattcagt agcgtctggc
    22561 agatcgacct ccacgctctg ggtgatgatg ggcgctgtcg ccataaagat cagcaacagc
    22621 accagcagta cgtccagcaa cggaacaatg ttgatttcgg acttgagatc gcgacgacct
    22681 cgtccacgcg ctctggccat ggcttacccc ttgttgctct cgctaacggt aaacgcctgg
    22741 cggtgcagaa tcgcggtaaa ctcttccata aagttgtcgt aattcagttc cagtttgttt
    22801 acgcgctggt tgagacggtt ataagccatt accgccggaa ttgcggcgaa cagaccaatt
    22861 gccgtcgcaa tcaacgcttc tgcgataccg ggcgcaacca tttgcagtgt tgcttgtttt
    22921 accgccccga gggcgataaa ggcgtgcatg atcccccaga ccgtaccaaa cagaccaata
    22981 tacgggctga tggagccaac cgtaccgagg aacggaatgt gcgtttccag attttcaagt
    23041 tcacggttca tggagatacg catagcacgc gacgcccctt ccactacggc ttccggcgca
    23101 tggctattgg cacgatgcag gcgcacaaac tctttgaacc cgctgtaaaa gatttgttcc
    23161 gaaccagtca gattatcccg tttcccctga ctctcttgat agaggcgaga gagttcgatt
    23221 ccagaccaga atttatcttc aaacgcttcg gcttcacgcg ccgctgcgtt aagaatacgg
    23281 gttcgctgga taataatggc ccaggatgcg attgaaaaac caatcaaaat caacatgata
    23341 agtttaacca gaaggctagc cttcaggaac aaatcaagga tattcatgtc agtcactgct
    23401 taaactccgc gacaatagac ttgggaagcg cacgaggctt cattttgagt gggtcaacgc
    23461 aaacaaccag aacctctgct tcattcagca aagtattctc ggcgttgaca atacgttgcg
    23521 tgaaaaccaa agaggtgcca cgcattgatg ttatttcagt ctgtatttcg agcatatcgt
    23581 cgagccgcgc aggtgcgtaa tattccaccg tcattttacg taccacaaag gcaacgcgtt
    23641 cagccatcag cgcctgctga ctgaagtgat gatgacgcag catctctgtg cgtgctcttt
    23701 cataaaaagc gacgtaactg gcgtggtaca ccacaccacc ggcatcggta tcttcatagt
    23761 agacgcgaac cggccatcga aacagcgttg tattcacttt acatcccggt aatgcaacaa
    23821 aagttagagc ttttaaacgc tgctactata cgcgcgcgat tgaggtttgg gaatgagggc
    23881 aagttaaggg cgcgtaaaaa tttatgggcc tggagcggcc cataattttt taaagagaat
    23941 tctgatttaa aagaagaaga aaatcagccc gacaatgaga acaatatcgg caagcaatgg
    24001 gcaaaaaatc ccttgccaaa gaaccttctg cggacgaaag cccacgccgt gaatcacacc
    24061 agcacagacg gcccacatca gcaataaacc atgccagatt tccagttcac tggtcttcgc
    24121 ggcaaaacga gatgggtccc aaaacataca tcctgctaac agaagcgcca tcacgaagga
    24181 aagcgcccgc agggggcgct tgtccattac cgcatacaag gtcgcgataa tcttactcat
    24241 cagatgtctt cttgaccggc tttgcctgat tcgacgtgtt caagcgccag cgcggtgatt
    24301 accccaaacg aacaggcaag aagcgttccc agaatccatg cgaaatacca catttttagc
    24361 tccttactta gtacagagag tgggtgttac gttcaatatc ttctttggtg atacgaccga
    24421 acattttcca gtaacaccag gcggtgtaga gcagaatgat cggtaccaga accaccgcaa
    24481 cccaggtcat gacgttaagc gtcagctggc tggaagttgc atcccacatt gtcagacttg
    24541 cgttcatcat ggtgctggac ggcatcacaa acgggaacat tgcgataccg gctgtcagga
    24601 tgatgcaggc cagcgtcagg gaggagaaca caaacgccca cgcggcttta tccatacgtg
    24661 cagtcaggat ggtcagcagc agcagaacca cacccagtgc cggaatagcc cacagaattg
    24721 gcgtgttgtt gaagttaacc agccatgcgc cagcttcacg aaccacttct ttattcagtg
    24781 ggttagaggc tgcgtaatgg tccattgtcg atttcacgac ataaccatcg ataccgtaca
    24841 tcacccatac gccagccagt gcgaaacaga ccagtgtcac cagcgcagcc acctgagccg
    24901 ttgcacgggt acgcaggtgc agttcgccca cggtacgcat ttgcagatag gttgcgccct
    24961 gagtaatgat catccctacg ctcaccacgc ctgccagcag gccgaacggg ttaagcaact
    25021 ggaagaagtt accggtgtag tacagacgca gatattcatc aacgttgaac ggtacgccct
    25081 gcaacaggtt accgaacgct acaccaatta ccagcggcgg aacgaagcta ccaatgaaga
    25141 tgccccagtc ccacatgtta cgccagcggg tttcttcaat cttggagcgg tagtcaaaac
    25201 cgaccggacg gaagaacaaa gacgccagca cgaggatcat cgccacatag aagccggaga
    25261 acgcagcggc atagaccatc ggccaggcag caaagagtgc gccgcccgcg gtgatcagcc
    25321 aaacctggtt accgtcccag tgtggtgcaa tggagttaat cataattcga cgctcggtgt
    25381 cgttacgacc gaggaaacgg gtgagcatgc ccacccccat gtcgaaaccg tcagtgactg
    25441 caaaaccaat cagcagaacg ccaaccagca gccaccagat aaaacgcaat acttcataat
    25501 cgatcatttg acgactcctg tcttagcgtg ccggctgagt agtcgtggaa gactgctcaa
    25561 agtgatagcg accggttttc aggctgcttg ggccgaggcg tgcaaacttg aacattaaga
    25621 acaattctgc caccaggaac agggtataca ggccgcaaat cagcaccatt gagaagatga
    25681 gatcgcctgc ggtcagtgac gaattcgcca cagctgtcgg cagcacttca ccgatagccc
    25741 acggttggcg gccatattca gccacgaacc agcccgcttc tacagcaatc cacggcagcg
    25801 gaataccgta cagcgcggcg cgcagaagcc attttttctc gccaatgcgg ttgcggatga
    25861 cactccagaa agagagcgcg atgattgcca gcagcaggaa gccacacgcc accatgatac
    25921 ggaacgcaaa gtacagcggc gctacacgcg ggatggagtc tttggttgcc tgttgaatct
    25981 gcgcttcagt cgcatcagcc acgtttggcg tatagcgttt cagcagcaga ccgtaaccga
    26041 ggtctttctt catgctattg aactggtcac gaaccgcctg gtcggtagaa ccagaacgca
    26101 gttgttcgag cagagagtac gccttcatcc cgttacgaat gcgttcttca tgctgcacca
    26161 tcagctcttt caggccgata accggggtat ccacggaacg cgttgcaatg atgcccagtg
    26221 cgtaagggat ctgaatcgca aatttgttcg tctcctcttc ctgatcagga atgccgaaca
    26281 aagtaaaggc agcaggcgca ggttgcgttt cccactcggc ttcaatagca gccagtttgg
    26341 ttttctgcac gtcgcccatt tcgtagccgg attcatcacc cagaacaata acagacagaa
    26401 cagcagccat accgaagctg gcagcgatag caaaggagcg tttagcgaag gcgaagtcac
    26461 gacctttcag catataccat gcgctgatac cgaggatgaa catcgcgcca gtcacataac
    26521 cagacgctac agtgtgaacg aatttcacct gagcaaccgg gttaagcacc agctcggaga
    26581 agctcaccat ctccatacgc atagtttcaa agttgaaatc ggacgcgatt gggttttgca
    26641 tccagccgtt cgcaaccaga atccacagtg cggacaggtt tgaaccgagc gccaccagcc
    26701 aggtgacaca catatgctga actttaccca gacgatccca accgaagaag aacagaccta
    26761 caaaggtgga ttcgaggaag aaggccatca gaccttcgat tgccagcggc gcaccgaaga
    26821 tatcccctac atagtgggaa tagtaagacc agttagtccc gaactggaac tccatggtca
    26881 gaccggtagc cacacccaga gcgaagttga taccaaacaa cttgccccag aacttggtca
    26941 tatctttata aatctgtttg ccggagagga cgtagaccgt ttccataatg gccaggagga
    27001 acgccatacc gagcgtcagt ggcacaaaaa ggaagtggta catcgcggtc aaggcaaact
    27061 gtaagcgcga cagttcgact atatctaaca tcatgactcc ttgctcatcg catgaagact
    27121 ccgagagtga accccgttag aaagggtcac acgcatgccc caatagaaat ttgtttgaac
    27181 actttcatca taccatgtga atgcacacaa aaacggcgta aagaaaggtt gcaaacatgt
    27241 taataaaaac tcaaattgat cccacgtata tattacgccg caaaatcctt acaataaaca
    27301 ggtttttgtt gaagcaaatt tgcatttttc gacggtgatc aatttatagc taaattgccg
    27361 cctttcagcc aatttgatcg agaacaattt atctcttttt gatgcccatt tccaagactt
    27421 atacattgat aaatatcaat ttctacaaat tatgttaatc atgtttcaac aaggtaactt
    27481 tagtgatatt tatgtttaaa atgtgtgaat tataagactg aaattagttt cggttggcaa
    27541 ttaaagataa aaagagacga agcaaaaaca gcacggttga ccttaattct ttttttggtc
    27601 actggctaat acgacgcaga gtttcaggat tattaataaa ggcataaatc tcccccccgc
    27661 cgatattaac atttaattaa cttataatta tattataatt aactcacccg tggttattat
    27721 tttgttaaaa ataaagccac atatacgtag cttgagagtg aagataaaca caaaattacc
    27781 tgatgcgcta cgcttgccag tgtctacatc atctctgcaa tatattgaat ttgcctgctt
    27841 ttgtagaccg aataaagcgt tcatgccgta tccggcatga acaaagcgcc ctttgtcaac
    27901 aatctggccg cgcatatgtg gcttcggttt gattgctatt caggcaagcc ggtaactgaa
    27961 cgtccgtgac tggccgggta aaaaaggccc cgatagattt gaaccttgat tttcctcggt
    28021 ggtaatgtgt tcatccatca tcgtttctga gcaagaaatc acctcgcgac tgaaagcaac
    28081 agtcgcatca caggttgctg attcagccgg attaaacagc cgtaaaatca cttcttgtcg
    28141 gtcttcagct ttcttaagtg cgcttatcag gcatcccact gggggcattt tcaacaaact
    28201 ataactttcc ggcacgttga atccggcttt gttgagcttc atcgcatccc atgggatttt
    28261 gttgtaacac tgtactggag tcagccatgc tcgcgcctgc tgagctacac cagcggcggt
    28321 tggcgtaccg gtataactca ataaacttag gcgacaagaa agcagaccac gtagttgtga
    28381 gtccgggact ggcattttaa ttcccgaagg ccgcctaggc cttaaaagca gatcttcttt
    28441 ccccagtaag cccacgccgc gcagcaacgt aatggcaaag gttttcttct cttcaccgat
    28501 gacttcaaat tcacgtagcc cttcgctaaa gacagccatg ccgttacgcc cttcctgtaa
    28561 ggctgcatag ttgagcatat tccataccgg aaccggcgct tctttccagc cttcttgctg
    28621 ccagttgttc attgcactgt cgttcacggg gcgcgttagc gaaccaaact gggtatctgc
    28681 cagaacacta tcggtgttaa aagatgtagg gatcaggacg cgaaggcgat gatcgtcagc
    28741 ctggttatca aggttgatat ccacatcaat gcgcctgttg ttatgactaa gagtgacaac
    28801 caactcgaca tctactctgc cagtggattg tcttgcgctg cgttgttgca gattacgcgg
    28861 caccgccatt tcatagcgga taacagccct gctctgccag gcctcatgaa taatctcgca
    28921 ttgcggtttc gcattcgccg aagtaattac ccactcttct tttgcgggtg aatagtcata
    28981 ttcatcacca tcatcagagc tttcttcaat ttgcaacacc cggtcatagc gcacaccgct
    29041 atctttatct accagttgta gcgttccatc ctcattgagc gcaatttgcc agaaagcatt
    29101 ttccagtatc ccttcagcgt cactttttgc cgcaatcacg ttgccaggct gattcgcttc
    29161 gatataaagc gtgcgatagc ccatagaagg gacaatctgg ttgatctgta tatcaaactc
    29221 cataaaggga tcgtaattac cgtaatgaac tatttgccga tcgattaggc ctggatcgat
    29281 ctcacgggca tggcgaataa aatacggtac aggctgaccg cgatcgtctc gtagattaaa
    29341 ctggctggcg cgcagccgca cagtggtgtt gatcacttct tcccgcggcc agggcatcag
    29401 gttaaacagg acgagtttgt cggcgtcgct ctgcggcatg ttgtcggcga ttttacgcat
    29461 atagaaacgc agcagattat ccgccatgtc ttcagccagt tcgaagcggg cgacgatttc
    29521 gcgatgaact ttgtcactac agcagcagcc gatgctgtcg tgggcatgat tttttaagat
    29581 ctctttccac attttctcca gcaagccgtg gtggtatcca aaacccaacg tcctggccag
    29641 cgttgccagc ggttccagca gattaacaat cttattttca atacgtgcgt gggcaacttt
    29701 gatatccatg cgcgtggaac cgatggtgcg atgcacgcgc atatatttgc catcaataaa
    29761 ttcccctttc agggttgcca gattatctcg ctgcgcttcg atcttctcaa atacctcttc
    29821 aaagcggctc atcacaaact ttcgttgagg ataaatttcg cgcagtttct ccatcacttc
    29881 gaagatattt tgctgcaatg gcatctgatc atgcccattc ggcagcaaaa tctctttggt
    29941 tacagacgct ttttccagca cgtcaaaata actgtcgagg cgtttgcgta atccgttttc
    30001 gtccgcaggt aagtacttac cgatggcata acccaacggc atcacctgcg ccgtcacttc
    30061 gctaccgtca ctgctttgcc acaaaaactc ggttttatcc gtaccgtggc gctcagaaca
    30121 tccacgccag aacatggtgc aggtaatgcc aaatccattg tagatatgcg gcagttgccc
    30181 ggacatgcca aaggaatcgg gtaaataacc tattttcatc ggctcgccaa acgcgaggca
    30241 gtcacgcatt ccgtacatca gattacggac gatggattcc gcagaaacaa tcgtggtatc
    30301 ggtctgggta taccaggggc cgataatcaa cttgccggct tctaccagtt tcttcacgcg
    30361 gtctttgttt tccggtttca ccgcgaaata atcttcgagg atcgccgttt gcccgtcgag
    30421 tacgtaatat ttgtattcgt tgtcctgttc cagtcggcac aggatctctt ccatattatt
    30481 gaccagcaga atgcgtgact cttcggtggt gaaataccac tcccgatccc agtgcatatg
    30541 cggggtgatg tgaacgcgag ataccgcttt catcgtcgtt tcctttttgt ttatggcatt
    30601 acgccatcag tcagatagtt gccatgctta accgcgtgac gccgccagat caggagaatt
    30661 gcagtcgaga ttgcagcccc caccaatgcc gcaccaaacc agccaattgc cgccataaca
    30721 ccgcccgcgc cattatcatg aagtaaaaag agcgagaaaa tgccagcacc gggtgtcgaa
    30781 aggccgatat tcatcgcacc gacaatagcg cccgttacca tagagcccag cacaaacgaa
    30841 ccaataaccc gcagcggatc ttcaatcgcc atcgggatcg ccccttcggt aatacctgcc
    30901 agccctaaca gccaggtgga tttcccggtc tcaatttcaa actctttaaa caggcgcggt
    30961 gcgagcatcg tggaagcggt tacggtaaat gccgaaacca ttttgacgga ggcgaaaatg
    31021 gcatacgggc cgtaaacgcc gttcgccatt gcgcccaggc agaatgcata agcggcttta
    31081 ttcactggcc ctccaaggtc aaaggaacac ataaaaccga gaatggcacc cagcaacagc
    31141 gcgttacttc ctgacagacc gttcagccag gcggtaagcg agttattgat ccaggcgaca
    31201 ggttccccca ccacaaacag catcagactg cccgctccca atgtaccgag caccgggtag
    31261 agataaaaag tcaggaagcc attgaattta ctgctaagac gcaagtgatt tttcatccag
    31321 cgtatcaagt aaccggctat caatccgcca acgaccgcgc cgagaaaccc ggagccgatc
    31381 atgttggcgg caagtccagc cgcaaagcct ggcgctaacg ccggtttatc tgccagcgaa
    31441 taggcggtat aggctgccag caccggtacc atcaaaattc cgagcagccc gccgcccagt
    31501 ttgcggtaca tccacagcca ggagttttct tcattaaaca gatcttgtag cccgaagatt
    31561 tgcgacagta ataccgcgac cgccagcacc gtcccccccg cgacaatcaa cggtacggca
    31621 aaagagatcc cgctcaacag tgcctgtttc agctccgttt tgacgctttt caccggttgc
    31681 gtatcttgct gtaaggtacg cgtctcatcg ccacgctcga gggtaagcga ttgttggatc
    31741 aacgcttctg catggcgaat cggctcggca acaggcacag aaagcgcagg aatgccgtta
    31801 aaacgctcac tctccttgat ggcgacttca gccgcaaaaa tacaggcagt tgcactattg
    31861 agttgatccg ccgttaaacg cccttcaata ccgttagcac cttgcttttc aacgtagaca
    31921 ttcacgccga gtttgcgtcc ggctttttcc agatactccg cagccatgta ggtgtgagca
    31981 atacccgccg ggcaggccgt tacgcaaacg atagttggcg cgttggaaaa agaggcagaa
    32041 ggttgcgtgc ctcccttgtc atccagcgcc gagagcaatt catcaggcgt cgtcgccgac
    32101 tgtatacgcg cccgaatctc atcatccgcc aggcgcgtgg tcagcgctgt cagcagttgc
    32161 atgtgcgtag tacccgcttc attgagggga atcgccagca acaccactaa atcaaccgct
    32221 tccgggccat ccacgccttc ccactgaagc ggctcgctga gtgtggcgac cgcaaacgcc
    32281 gcttctttta ccgcagcagt tttgccatgc ggcacagcca acccttcact taaggcggtc
    32341 gggccaaggc tttcacggcg atacacttct ttcagaaatt gctcagtact ggaaattttc
    32401 cccagagcag caagacgttg agtcaacgcg tgaatggcct cttcacggct ggtaaagcgc
    32461 gcattcagac acaacgcatc gcggtgggtc agagtcgtca ggttcatcat gatcctcact
    32521 tgatcgtttt tatagtcgcg ccagtgtgcc cgacaaaaca ataccattgc gtaatcaatt
    32581 tcacttgatt gaaattattt gcattttatt tgtattgttt acaggatgaa tttatcgggc
    32641 ataatggcta aattcgtatt aatgagatgc taaataatgg gacacaaacc cttataccga
    32701 cagattgctg atcgcattcg tgagcaaatt gcgcgtggcg agcttaaacc tggcgatgcg
    32761 ttacctaccg aatcggcatt acaaacagag tttggcgtca gccgggttac ggtgcgtcag
    32821 gctttgcgcc agttagtcga gcaacagatc ctcgaaagca ttcagggcag cgggacttac
    32881 gtcaaagaag agcgggtcaa ttacgatatt tttcagttaa ccagttttga tgaaaaactg
    32941 tcggaccgtc acgtcgatac acacagtgaa gttctgatat ttgaagtgat tccggctgac
    33001 gattttcttc agcaacagct acaaatcacc gtgcaggatc gtgtgtggca tgtgaagcgc
    33061 gtgcgctatc gcaagcaaaa gccaatggcg ctggaagaaa cctggatgcc gcttgcgttg
    33121 ttcccggatc tcacctggca ggtcatggaa aattcgaaat atcactttat tgaagaagtg
    33181 aagaagatgg ttatcgatcg tagcgaacag gaaatcattc ctctgatgcc aaccgaagag
    33241 atgagccgtc tgcttaatat cagccagaca aagccgattc ttgaaaaagt atcgcgtgga
    33301 tatttagttg atggtcgggt gtttgaatat agccgcaacg tttttaatac cgatgactat
    33361 aaatttaccc tgatagcgca gcgaaaatca tcgcgataag cacaaaaagg ccatcatacg
    33421 atggccaacc atgtcgaaac cgacagccct tcgccattcc tgtagaggga agcggcgagg
    33481 gctatttctt attacagata tttatttcag aacagttttc agtgcttcac cgatatccgc
    33541 caggctgcga acggttttca cgcctgcggc ttccagagca gcgaatttct catccgcagt
    33601 ccctttccca ccggcaatga tcgcacccgc gtggcccata cgtttgcctt tcggcgcagt
    33661 cacaccagcg atgtaaccca caactggctt ggtaacatgc tctttgatgt acgctgccgc
    33721 ttcttcttca gcgctaccgc cgatctcacc gatcatcacg atcgcttcgg tctgcggatc
    33781 tttttcgaac atttcgagaa tgtcgataaa gttagagcca gggatcgggt caccgccgat
    33841 accgacacag gtcgactgac cgaaaccgta atccgtggtc tgtttaaccg cttcataggt
    33901 cagtgtaccg gaacgggaaa cgatacccac tttacccggt ttgtgaatgt gacccggctg
    33961 gataccaatt ttgcattcac ccggggtgat aacgcctggg cagttcgggc cgatcatacg
    34021 aacgcctgct tcgtccagtt tcactttcac ggtcagcata tccagcgtcg ggatgccttc
    34081 agtgatggtg ataatcagtt tgatgcctgc atcgatagct tccagaatgg agtctttgca
    34141 gaacggtgct ggtacgtaaa taacagacgc ggaagcgcca gtagcagcaa cagcttcacg
    34201 cacggtgttg aacaccggca ggccgaggtg ggtagtgccg cctttacctg gagttacgcc
    34261 accaaccatt ttagtgccgt atgcaatggc ctgttctgag tggaaagtcc cctggctacc
    34321 ggtaaagccc tggcagataa ccttggtgtt tttatcaatt aaaatggaca ttatttcccc
    34381 tccactgcgg caacaacctg ctgagctgca tccgtcagac cttttgctgc aataatattc
    34441 aggccgctgt cagccagttt cttcgcgccg agttcggcgt tgttaccttc cagacgtacc
    34501 acgaccggta cgttaacacc cacttccgct accgcgccga tgataccgtc agcgatcagg
    34561 tcgcaacgaa cgataccgcc gaagatgtta accagaacgg ctttcacttt gtcgtcagag
    34621 aggatgattt tgaacgcttc ggttacacgt tctttggttg cgccgccgcc aacgtccagg
    34681 aagttagccg gttcgccgcc gtgcagttta actatgtcca tcgtacccat cgccagacct
    34741 gcgccgttaa ccatacaacc gatgttaccg tccagcgcaa cgtagttcag ttcccactgt
    34801 gcagcctgtg cttcacgcgg atcttcctgc gactggtcac gcatttcgcg cagatcaggc
    34861 tggcggaaca gggcgttgcc atcagcgccc agtttgccgt cgaggcaaat cagatcgccc
    34921 tgtttggtga tgaccagcgg gttgatttcg atcagcgcca ggtcgcgctc caggaaaatg
    34981 gtcgccaggc ccatgaagat tttggtgaac tgctgaacca gtttaccttc cagacccagt
    35041 ttgaacgcca gctcgcgtcc ctgatacggc atcgggccag tcagcggatc aagcgcaact
    35101 ttatggatca ggtgcggagt ttcttccgcc actttttcga tttccacgcc gccttcggtg
    35161 gaggccataa agaccacgcg acgggaacta cggtctacaa cggcaccgag atacagctct
    35221 ttagcgatat cggtcgccgc ttcaaccaga atctggttaa ccggttggcc attggcatct
    35281 gtttgatacg ttaccagacg cttgcccagc cagttttctg caaaagcacg gatgtcttct
    35341 ttgctgttta caactttcac accgcccgct ttaccgcggc caccagcgtg aacctgacat
    35401 ttcactaccc acggaccggc accgattttt gaagcggctt cttctgcttc gcgcggagta
    35461 gtacaggcat aacccaccgg tgctggtaag ccatagcggg caaacagttg ttttgcctga
    35521 tattcatgta agttcatgtg ttctgtccat ccttcagtaa tcgttatctt ttaaaccgta
    35581 gaccgacacc ctatccacgt cggtctacag tgcaggtgaa gcttaaacta ctacacgtcc
    35641 agcagcagac gcgtcggctc ttccagtaac tctttgatcg ttaccagaaa gcccacggat
    35701 tcgcgaccat cgatcagacg gtgatcgtag gacagcgcca ggtacatcat cggcaggatc
    35761 tcaacctgac cattcaccgc catcggacga tctttgatag cgtgcatacc cagaattgcg
    35821 ctctgcggcg ggttgatgat cggcgtagac atcagggaac cgaacacacc accgttggtg
    35881 atggtgaagt taccaccggt cagatcttca accgtcagct tgccatcacg gcctttgact
    35941 gccagctctt tgattttctt ctcgatgtct gccatgccga gggtatcgac atcacgcaga
    36001 accggcgtca ccaggccgcg cggcgtagaa accgccatgc tgacgtcgaa atagttgtgg
    36061 taaaccacgt catcgccgtc gatagacgcg ttcacttccg ggtaacgttt cagggcttca
    36121 accaccgctt tcacgtagaa ggacataaag cccagacgga tgccgtggcg tttttcaaac
    36181 gcttcaccgt actgcttacg cagatccata atcggcttca tgttgacttc gttgaacgtg
    36241 gtcagcatgg cggtggagtt tttcgcttcc agcagacgct ctgccacacg cttacgcagg
    36301 cgagtcatcg ggacacgttt ttcactacgt gcagccagag ccggttgcgc cgccggagca
    36361 gccgctgccg gagcagactc tttcgccggg gctttcgcca gatgtttttc cacatcttca
    36421 cgagtcagac gaccacccac accggtgcct ttaatggcgc tggcgtcgag attgtgttca
    36481 gccagcaggc gacggatcgc cgggcttaac gcatcgttgt tttgctcttc cagagacgcc
    36541 tgctggcgtt gcgccggagt ggacgctttc tcttcagatt tggcgctggt ttctttaccg
    36601 gcgctgttgc cttcacgcag gcgaccaagg atctgacgag acgttaccgt tgtaccttca
    36661 tcttccagaa ccgcatccag aatgccgtct gctgatgccg gtacttccag taccactttg
    36721 tcagtttcga tttctaccag cacttcatca cgtacgactg cgtcgccggg ttttttatgc
    36781 caggttgcga cggtggcatc ggctacggat tcaggcaggt cagggaccag aatatctacg
    36841 ctactcattg tgtatccttt atttattcga cgttcagcgc gtcattaacc agatcttgtt
    36901 gctgtttctg gtgaacggac atatacccta ccgccggaga ggcggaggcc gggcggcctg
    36961 cataacgcag agaagcccca aacggaatca cttcacggaa atgatgctgg ctgcagtacc
    37021 atgcgccctg gttgagcggc tcttcctggc accagacaaa atccttgacg tgagcaaact
    37081 gctgcaacac ttcctgcatc gctttatgcg ggaacgggta gagttgctcg atacgcacaa
    37141 tggcgacatc gtgttgattg ttcttacgac gctgttccag caggtcgtaa taaaccttac
    37201 cagaacacat cactacgcgc ttcacgccct tcggatcaag ctcgtcgatt tcaccgatgg
    37261 ctggcaggaa ggtgccgttc gccagttctt cgaggctgga aaccgccagc ggatgacgca
    37321 gcagggattt cggcgacatc acgaccagcg gacgacgcat cccgcgcagc gcctgacgac
    37381 gcagcatgtg gtaaacctgt gccggggtag acggtacgca aacctgcatg ttttgctcag
    37441 cacaaagttg cagataacgt tccagacgcg cggaggagtg ctccggcccc tgcccttcgt
    37501 aaccgtgcgg cagcaacatc accagaccac acatccggcc ccatttctgc tcgccagagg
    37561 agatgaactg gtcgataacc acctgcgcac cgttggcgaa gtcaccgaac tgcgcttccc
    37621 agatggtcag ggtgcgtggt tctgcggtgg cataaccata ttcaaacgcc agcacggctt
    37681 cttctgacag tacggagtcc cagacacgga acgcgccctg accgttatgg atgtgttgca
    37741 gcggcgtgta agtggaaccg ttagactggt tgtggatcac cgcgtggcgg tggaagaagg
    37801 taccgcgacc ggagtcttca cccgacaggc gaaccggaat gccttcatca accagcgtgg
    37861 cgtaagcgag gttttccgca ccgccccagt cgaacagttt ctcaccggct gccatcgcct
    37921 ggcgatcgcc ataaatcttg gcaacgcgag actgcatttc aactgcttct ggtaccgtac
    37981 tgatacgttt agccagttcc tgcaggcgct tcatctcaac tttgttcggg tactcttcgt
    38041 cccattcgtg gttgaggtac ggcgaccagg tgaaagagtg catgttcatc ggacgccact
    38101 ctgccactac gcaatcgcca gcatccagcg catcgcggta caggttaacc atctcggtgg
    38161 catcttccag cgtcgccact ttttcctgct ccagcttgtc agcgtagatt ttgcgcggtg
    38221 tcggatgttt tttgattttc tgatacatca gtggctgggt tgcgctcggc tcgtcggctt
    38281 cgttgtggcc gtgacggcgg tagcatacca ggtcgatgaa gacatcacgt ttaaaggtgt
    38341 tacggaaatc gagcgccaga cgggtcacaa aggcaacggc ttccggatca tccgcgttaa
    38401 cgtggaaaat cggtgcctga accatcttac cgatatcagt acagtacggt gtagaacggg
    38461 catccagcgg gttagaggtg gtgaaaccaa cctggttgtt gataacgata cgtaccgtac
    38521 cgccaacttc ataaccacgc gctttcgaca tgttcagagt ttcctgaacc acgccctgcc
    38581 cggtcactgc ggcgtcaccg tggatggtga ttggcagcac tttgttgctg ttcggctcat
    38641 caagtctgtc cagacgggca cgaacagagc cgataacgac cgggcttaca atctcaaggt
    38701 gagacgggtt aaacgccagc gccaggtgca ccaggccgcc atcggtctgg aagtcagacg
    38761 agaagcccat gtggtatttc acgtcgcctg tgccgaggtg ttctttatgt ttaccggcga
    38821 actcgtcgaa caagtcttgc ggttttttac ccagcacgtt caccagcacg ttcagacgac
    38881 cacggtgcgc catcccgaga accacttcgc gggtgccgct gttgccagcg tggcggatca
    38941 tctctttaag catcgggatt aacgcgtcac cgccttccag cgagaagcgt tttgcgccag
    39001 ggaattttgc gccgaggtaa cgctcaaggc cttcagcggc ggtcagttcg cttaagaagc
    39061 gttttttctc ttcgctattg aaagtcgcgc gaccagactc gatacgctgt tggatccagc
    39121 gtttttcttc ggtgctggtg atgtgcatat actcggcacc aatcgggccg cagtaggttt
    39181 gcttgagggc ggaaataagc tcgccgagtt tcatggtttc tttgccgctg gcaaatgaac
    39241 cgacgttgaa ggtttcctgg aagtctgctt cggtcagatc gtggaaagac ggatccagat
    39301 cggccacttt atcttgctgc cacagtccca gcggatctag attcgcatgc tggtgaccac
    39361 ggaagcggta tgcgttaatg agctgcagga ctttaacctg cttcacattg gtgtcagggt
    39421 cggagatcgt tgaagagtaa cgtgaagcgt ctttcgccag gcggcggaaa tattcacgcg
    39481 tttgagagtg gaattgatcc ggtttgactc ccgtaccagg taactgctgg aacgtcgaac
    39541 gccagttagc gtcaaccgag tcaggatcgg ttaagaagtc ttcatagagc tgttctatcc
    39601 agctctggtt tgcgccagag aggtaagaag agtccaacca ggctttcaaa gcgctgttct
    39661 gcatcgtgat cccttaagca tcttttttat gcttacttcg ccgtggatac taccacgcac
    39721 agtgatgtgc gtgccggggt tcacttgcga gctcttgtct acgtagtggc tcgcgaagga
    39781 acctttaaaa actgtcttag tgtcaggggc agtttttaaa gatttcctgc atttctttgt
    39841 tacgcctgat gcgctgcgct tatcaggcct gcgggtaatg acttgtaggc cggataaggc
    39901 gttcacgccg catccggcac tggttgcctg atgcaacgct tgcgcgtctt atcaggccta
    39961 cagtttacgc attacgttgc aacaacatcg acttgatatg gccgatggcg cgcgtcgggt
    40021 tcagcccctt cggacataca ctgacgcagt tcatgatgct gtgacagcgg aatacgctga
    40081 atgcatcgct caaaccgtcg aggcggctgt cagtctcggt atcacggcta tcgatcagga
    40141 aacgatatgc cgctaacaag cctgccgggc cgataaactt atcgggattc caccagaaag
    40201 acggacaaga ggttgaacaa catgcgcaga gaatacattc atacagcccg tcgagttttt
    40261 cgcgctgctc tggcatctgt aaatgctcgc gagctggcgg attttgtcca ttattcaaca
    40321 ggtaaggctt aattttctca tattgcgcat agaattgtcc catgtctacc accaaatcgc
    40381 ggatcaccgg taaacctggc agcgggcgaa tcacaatctt cttgcccggc tggttgagtg
    40441 ccgaaatcgg ggtaatacag gccagaccat tcttgccgtt catgttcaga ccgtcggaac
    40501 cgcacacacc ttcacggcag gagcggcgga acgacaggct gggatctttc tctttcagct
    40561 ggataagcgc atccagcagc atcatgtcgc gaccttcttc cgcttccagg gtgtaatcct
    40621 gcatacgcgg agcatcatca acatccgggt tatagcgata aattgaaaac tcgagtctca
    40681 ttttcctgtc tccgcattag taagtacgaa tcttcggcgg gaatgccggg cgcagtttcg
    40741 gctccatgtt gacgcttcgg cgcgtcatgg attccgactc aggcagatac agggagtggc
    40801 acagccagtt ttcatcatca cgatccggga agtcgaagcg gctatgtgcg ccacggcttt
    40861 cggtacggaa gttggcagaa acagccgttg catacgccgt ttccatcagg ttatccagtt
    40921 ccaggcactc aacgcgctgg gtgttgaact cgctggaagt gtcatccaga cgggcatttt
    40981 tcagacgctc gcggatcact ttcaactgct caagcccttt cgccatcgca tcaccttcac
    41041 ggaagaccga gaagttatgc tgcatacatt cttgcagcgc tttacggatc gccaccggat
    41101 cttcaccgtt acgattattg ttccaccggt tcaggcgatc cagagacgct tcaacatcag
    41161 actcgctggc atcgtgcagt gcgccctgct cggcgataga ctcttgcaga tgcagacctg
    41221 ccgcgcgacc aaagaccacc aggtccagca gcgagttgcc gcccagacgg ttagcgccgt
    41281 gtaccgatac acaagcgatt tcaccaacgg caaacagtcc cggaacaacc acatcttcgc
    41341 ctttctcatt cacagtcagc gcctgaccgg taactttggt cggaataccg cccatcatgt
    41401 agtgacaggt tgggataacc ggaatcggct ctttcaccgg atcgacgtga gcgaaggtac
    41461 gggaaagctc caggataccc ggcagacggg attcgagaac ttctttaccc aggtgatcga
    41521 gtttcagttt cgcgtgtggc ccccacggac catcacagcc gcgaccttca cggatttcaa
    41581 tcatgatgga acgcgccacc acatcacggc ccgccaggtc tttggcgttc ggcgcataac
    41641 gctccataaa gcgttcgcca tgtttgttca gcagataacc gccttcacca cggcaacctt
    41701 cggtgaccag tacgcccgca ccggcaatgc cggtcgggtg gaattgccac atttccatat
    41761 cctgcaccgg tacgcctgcg cggatagcca tgccgacacc gtcgccagtg ttaatgtggg
    41821 cgttagtggt ggactgataa atacgccctg ctccgccagt cgccagcacg gtagcgcggg
    41881 ctttgaaata aaccacttca ccggtttcga tgcacagtgc ggtacaaccc accaccgcgc
    41941 catcctggtt tttcaccaga tccagcgcat accactcgga gaaaatggtg gtgtggtttt
    42001 tcaggttctg ctgataaagc gtgtgcaaca gtgcgtgacc ggtacggtca gcagccgccg
    42061 cagtgcgtgc cgcctgctcg ccgccgaagt ttttcgactg accgccaaac ggacgttgat
    42121 agatacgacc atcatcgaga cgcgagaacg gcaggcccat gtgttcgagt tccagaatcg
    42181 cttccggccc ggttttacac atatattcaa tcgcgtcctg gtcaccgata tagtccgacc
    42241 cttttacggt gtcgtacata tgccattccc agttatcttc atgggtatta cccagcgcaa
    42301 cggtaatgcc gccttgcgca gaaacggtat gggaacgggt cgggaagact ttagagagca
    42361 gggcacaggt ctggccgctc tgggaaattt gcagcgccgc gcgcatacct gcgccaccgg
    42421 caccaatcac aactgcatca aattctctga ctggcaattt catcacacac cccacaccac
    42481 aacgaatcca taaatcacgt aaaccaccag tgcaacgaca atcaccagtt gcagcatcag
    42541 gcgcaaggcc agcggtttaa cgtagtcggt caacacctgc cacatgccga tccaggcatg
    42601 aatcaagata gaaaacagcg ccagcagggt gaacactttg gtgaacgcag aggcgaagaa
    42661 accgatccag acttcatatg tcagttcgcc actggtggcg aaaaaaccga ccatataaat
    42721 gatgtagagc gtcaggacga tagcggtagc gcgaacgagg atgaaatcat gtacgccatt
    42781 gcgtcctaat gcggaggcgt tgcttaccat acgaggactc ctgcgagaag tgaaagcacg
    42841 acagtaataa caaaggagat tttggcggag cgtttacccg cttcgaatgt ttcttccaga
    42901 tagccaaaat ccatcatcat gtggcgaata cctacgacga cgtgatacgc cagagcggta
    42961 aggatgcccc acatgataaa tttgacgaag aagctgccca taatcgcgga agcttgctcg
    43021 aaaccttcag gggaagagag gctggtaccc agaagccact gcaggatgcc cactgcaaca
    43081 aaggtgatca caccggaaac gcgatggaga atggacgcta tcgccgtgat ggggaaccgg
    43141 atggtctgta ggtccagatt aacaggtctt tgttttttca catttcttat catgaataac
    43201 gcccacatgc tgttcttatt attccctggg gactacgggc acagaggtta actttctgtt
    43261 acctggagac gtcgggattt ccttcctccg gtctgcttgc gggtcagaca gcgtcctttc
    43321 cataactgcg cgtcatgcaa aacactgctt ccagatgcga aaacgacacg ttacaacgct
    43381 gggtggctcg ggattgcagg gtgttccgga gacctggcgg cagtataggc tgttcacaaa
    43441 atcattacaa ttaacctaca tatagtttgt cgggttttat cctgaacagt gatccaggtc
    43501 acgataacaa catttattta atttttaatc atctaatttg acaatcattc aacaaagttg
    43561 ttacaaacat taccaggaaa agcatataat gcgtaaaagt tatgaagtcg gtatttcacc
    43621 taagattaac ttatgtaaca gtgtggaagt attgaccaat tcattcggga cagttattag
    43681 tggtagacaa gtttaataat tcggattgct aagtacttga ttcgccattt attcgtcatc
    43741 aatggattct ttacctgcaa gcgcccagag ctctgtaccc aggttttccc ctctttcaca
    43801 gagcggcgag ccaaataaca aacgggtaaa gccaggttga tgtgcgaagg caaatttaag
    43861 ttccggcagt cttacgcaat aaggcgctaa ggagacctta aatggctgat acaaaagcaa
    43921 aactcaacct caacggggac acagctgttg aactggatgt gctgaaaggc acgctgggtc
    43981 aagatgttat tgatatccgt actctcggtt caaaaggtgt gttcaccttt gacccaggct
    44041 tcacttcaac cgcatcctgc gaatctaaaa ttacttttat tgatggtgat gaaggtattt
    44101 tgctgcaccg cggtttcccg atcgatcagc tggcgaccga ttctaactac ctagaagttt
    44161 gttacatcct gctgaatggt gaaaaaccga ctcaggaaca gtatgacgaa tttaaaacta
    44221 cggtgacccg tcataccatg atccacgagc agattacccg tctgttccac gctttccgtc
    44281 gcgactcaca tccaatggca gtcatgtgtg gtattaccgg cgcgctggcg gcgttctatc
    44341 acgactcgct ggatgttaac aatcctcgtc atcgtgaaat tgccgcgttc cgcctgctgt
    44401 cgaaaatgcc gaccatggcc gcgatgtgtt acaagtattc cattggtcag ccatttgttt
    44461 atccgcgcaa cgatctctcc tatgccggta acttcctgaa tatgatgttc tccacgccgt
    44521 gcgaaccgta tgaagttaat ccgattctgg aacgtgctat ggaccgtatt ctgatcctgc
    44581 acgctgacca tgaacagaac gcctctacct ccaccgtgcg taccgctggc tcttcgggtg
    44641 cgaacccgtt tgcctgtatc gcagcaggta ttgcttcact gtggggacct gcgcacggtg
    44701 gtgctaacga agcggcgctg aaaatgctgg aagaaattag ctccgttaaa cacattccgg
    44761 aatttgttcg tcgtgcgaaa gataaaaatg attctttccg cctgatgggc ttcggtcacc
    44821 gcgtgtacaa aaattacgac ccgcgcgcca ccgtaatgcg tgaaacctgc catgaagttc
    44881 tgaaagagct gggcaccaaa gatgacctgc tggaagtggc tatggagctg gaaaacatcg
    44941 cgctgaacga cccgtacttt atcgagaaga aactgtaccc gaacgtcgat ttctactctg
    45001 gtatcatcct gaaagcgatg ggtattccgt cttccatgtt caccgtcatt ttcgcaatgg
    45061 cacgtaccgt tggctggatc gcccactgga gcgaaatgca cagttacggt atgaagattg
    45121 cccgtccgcg tcagctgtat acaggatatg aaaaacgcga ctttaaaagc gatatcaagc
    45181 gttaatggtt gattgctaag ttgtaaatat tttaacccgc cgttcaaatg gcgggttaat
    45241 ttttatatgc ctaaacacaa aaaattgtaa aaataaaatc cactatcgga cattcatata
    45301 ttctaaaaaa aaatacatcg atataaatta taaacaaata gatgattttt aatcatctat
    45361 cttcctctca gttacatgaa taaaccacca aatatagcgc acccctataa ttcccaatac
    45421 tttattcatc aaacatttaa ttcataccat caattaatta cttttacatt taataaaatt
    45481 aataatattt ttaagaaaaa ctaaaaacat tcgacaatca aataagatat tcttgcaccg
    45541 atatactttt ttcttttctg ttaaaggact cagttatgtt taaaggacaa aaaacattgg
    45601 ccgcactggc cgtatctctg ctgttcacag cacctgttta tgcggctgat gaaggatccg
    45661 gtgaaattca ctttaaaggt gaagttattg aagcaccgtg tgaaatacat caggatgata
    45721 ttgataaaga ggttgaactc ggtcaggtga ccaccagcca cattaatcag tcacatcaca
    45781 gcgatgccgt tgctgtcgac ctgctcttag tcaactgtga tctggaaaac tccagcaacg
    45841 gttccggtgg caagatttct aaagttgcag tcacctttga tagctcagcg aaaaccaccg
    45901 gcgcagatcc gattctcaac aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt
    45961 taatgaataa agatcaaagc aatatcgttt taggcaccgc tactccagat atcgacctgg
    46021 ctccgacctc cagcgaacaa acgctgaatt tctttgcctg gatggaacaa attgatcagg
    46081 ctacacctgt aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg gattataaat
    46141 aagtattact ttggcggcgc tatcgccgcc tttcataaaa tatttatcag gtatggacac
    46201 cgtgaatatt tatcgactct cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt
    46261 ggcggtcgaa ttcaatctga atgttctcga taaatcaatg cgcgaccgca ttgatatttc
    46321 attattaaag gaaaaaggag tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa
    46381 taataaccaa atcagtaacg agcaaaagat taactggcac aaaaatgacg ataaaaccat
    46441 tccgtgcatc aatgatttac tggtcgataa atttggctta aaacctgaag tccgtcagtc
    46501 gttaccattg ataaatcagt gcgtcgattt tagctcccga cctgaaatgc tcttcaattt
    46561 cgatcaagcc aatcagcaac tgaatattag tattccgcaa gcctggctgg cgtggcactc
    46621 agaaaactgg accccaccct ccacatggaa agaaggtgtc gccggtgtcc tgatggatta
    46681 caacttgttt gccagcagct accgcccaca ggatggcagc agcagcacta atcttaacgc
    46741 ctatggaaca gccggaatca acacgggggc atggcgctta cgcagtgatt atcagttgaa
    46801 tcatactgat agcgatgata accatgaaca gtcaggcgaa atatcacgta cctatctttt
    46861 tcgcccgtta ccgcaattag gttcaaaatt aaccctcggc gaaacggatt ttagtcccaa
    46921 tattttcgac ggtttttctt ataccggcgc ggcactggca agtgacgatc gaatgttgcc
    46981 atgggaacta cgcggctacg ccccacaaat tagcggtatt gcacagacca atgccacggt
    47041 gacgatcagt caatcaggcc gcgtcattta ccagaaaaaa gtcccaccag gcccatttat
    47101 cattgacgac cttaatcagt ctgttcaggg cacactggat gtcaaagtga cggaagaaga
    47161 tggtcgggtg aacaatttcc aggtttcagc agcatcgacg cccttcctga ctcgtcaggg
    47221 acaggttcgc tataaactgg ccgcgggtca gccacggccc tccatgtcac atcaaactga
    47281 aaatgaaacc ttttttagca atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta
    47341 cagcggcctg ctgctttctg gtgatgacta ccattctgcc gcaatgggta ttgggcaaaa
    47401 tatgctgtgg cttggtgcgc tgtcgtttga tgtcacgtgg gccagtagcc attttgatac
    47461 tcagcaggac gagcggggct taagctaccg ttttaattac agcaaacaag tggatgccac
    47521 taacagcacg atttccctcg ccgcttatcg tttctccgat cgtcattttc acagctacgc
    47581 caactatctg gatcacaaat acaacgacag cgatgcgcag gacgaaaaac agacgatcag
    47641 cttatctgtg ggccaaccga ttaccccact aaacctcaat ctttacgcca acctgctaca
    47701 tcaaacctgg tggaatgcag acgcctccac gaccgccaac atcacggcag gttttaatgt
    47761 tgatattggt gactggagag atatctctat ttctacgtca ttcaatacaa cccattacga
    47821 agataaagat cgcgacaacc agatttacct gtcgatttcg ctccccttcg gtaacggtgg
    47881 tcgggttggc tatgacatgc aaaacagtag ccacagcacc acacaccgca tgtcgtggaa
    47941 cgatacgctg gatgaacgta atagctgggg catgtctgcc ggactgcaat ccgaccgtcc
    48001 tgacaatgga gcccaggtga gcggtaacta tcagcacctg agttcagcgg gtgagtggga
    48061 tatttctggt acctatgccg ccaatgatta cagttccgtc agcggcagct ggagcggttc
    48121 tttcaccgca acccaatatg gtgcagcgtt tcatcgccgc agctccacca atgaaccacg
    48181 cctgatggtc agcaccgatg gcgtggcaga tattccggtt cagggcaatc tcgactacac
    48241 caaccatttt ggcattgcgg tggtgccgtt gatttccagt taccagcctt ccaccgtggc
    48301 ggtgaacatg aatgacttac ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac
    48361 gtggattgaa ggcgcgatag gttacaaatc actggcttcc cgttccggta aagacgttaa
    48421 cgtcatcatt cgcaacgcca gcggtcagtt tcctcccctc ggagcggata tccgccagga
    48481 tgacagcggc attagcgtgg ggatggttgg cgaggaagga catgcctggt taagcggagt
    48541 cgctgaaaat caaaagttta ccgtggtctg gggtgatagc cagcattgct cgctccatct
    48601 tcctgaacat atggaagaca ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa
    48661 atgaaaatga catttatgaa aggactaccc cttttgttgt tggttgccag tctgtgcagc
    48721 cacgctgcac tacaacccga tcgcactcgt attgtgttta acgctaatga taaagctacc
    48781 agtctgcggg tggataatcg tagcgataaa ctcccctacc tcgcttattc ctggctcgaa
    48841 aatgaaaaag gggagaaaag cgatgatctt ctggtcgccc tgccgccgat acaacgtctg
    48901 gaaccgaaag caacaacaca ggtacggata gtaaagcaag cctcgaccac aaaattaccg
    48961 ggcgatcgcg aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa
    49021 aacagcgacc atgccgttct gcaagtcgcc attcagagtc gtatcaaggt gttctggcga
    49081 ccggctgcat tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca ggtcagccag
    49141 cagggcaacc aactgacact gaaaaatcct accgcgtatt atctgaccat tgcctacctg
    49201 ggacgaaatg aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc gccttttagt
    49261 acggtcaaca caaacaccgg aaattatagc ggcagccagt tttatctcgg ttatatggat
    49321 gattacggcg cgttacgcat gacgacgctc aactgcagcg gacaatgcca tttacaagcc
    49381 gtggaggcga agaaatgagt gctggcaagg gagtgttgct cgtcatctgt ctgttatttc
    49441 tgtcgttgaa gtctgcgctt gccctgaact gctactttgg ttcatccggt ggctcggtag
    49501 aaaaatcaga agcgattcaa ccttttgctg taccaggcaa tgccaaactc ggcgataaga
    49561 tctgggaatc tgacgatatt aaaataagat ctgggaatct gacgatatta aaattcccgt
    49621 ctattgtgac aacaatacca acggcaactt tgaaagcgaa catgtctacg catgggtaaa
    49681 cccctacccg ggggtgcagg atcgttatta tcaactcgga gtaacttaca atggcgttga
    49741 ttatgacgcc aaccagggaa aaagccgcat cgacactaac cagtgtatcg acagtaagaa
    49801 cattgacatt tatacccctg atgggatggc agaacaaaat ttgctcgggt gatcccgcta
    49861 atattcacat gtcgcgaacc tttctcgctc gcatgcggtt atacgtcaaa atacgagaaa
    49921 tgccgccgca tgattatcaa agtacgctta gcgactatat cgtcgtgcaa tttgacggtg
    49981 ccggtagcgt taacgaagac cctactgccc aaaacctgaa atatcatatt actggtctgg
    50041 aaaacattcg cgtgctggat tgcagcgtca atttttccat ttcaccggaa acacagatga
    50101 ttgattttgg taaatttaat ttgctggata ttcgtcgcca cacaatgtcg aaaacattca
    50161 gtattaagac gacaaaatca caaaatgacc aatgcaccga cggatttaaa gtcagttcct
    50221 ccttttatac cgaagaaacg ttggttgaag aagataaagc attgttgatt ggtaatggtc
    50281 tgaagctacg tttgctggat gaaaacgcct cgccttatac cttcaataaa tatgctgaat
    50341 acgccgattt caccagcgac atgttggtct acgaaaaaac ctatacagct gaactttcgt
    50401 ccattgcagg tacccccatc gaggctgggc ccttcgatac agtggtgctt tttaaaatta
    50461 actataactg atttgccatg atgccctttg cagccggaaa gggcatctct ttcagaaaat
    50521 tgctgatttt ccccttttcc ttgattgctg ttattttttc ctagcccggc tttctttatg
    50581 ctgtatcgat acaccaaaga gaaaataatg agggagcgaa ggatgccagt tttgcagtgg
    50641 ggaatgttat gtgtgctatc acttctcctt tctattggtt tcctcgcggt acatctcccg
    50701 gcggcgctat tacttgggcc gatgatcgcc gggatcatct tcagtatgcg cggaattact
    50761 ctgcaacttc cccgctccgc ttttctcgcc gcgcaggcca ttcttggctg catgattgcg
    50821 caaaacctca ctggttcgat tctcaccacg ctggccgcca actggccgat cgtgcttgct
    50881 attttactgg tgacgctgct ttccagcgcc atcgtgggtt ggttattggt gcgctatagc
    50941 tcactgcctg gaaataccgg tgcctggggt tcttctccag gcggcgcagc ggcaatggtc
    51001 gctatggcgc aagattacgg cgcagatatt cgcctggtgg cgtttatgca atatctgcga
    51061 gtgctgttcg tcgcgggggc cgctgttctg gtcacccgca tgatgctggg tgataacgct
    51121 gaagcggtta accagcaaat cgtctggttc ccgccagtga gcattaatct cctgcttacg
    51181 attttgctcg cagtcgttgc cggtacggcg ggatgcatgc tgcgtcttcc ttccggcacg
    51241 atgctcatcc cgatgctggc gggcgcagtg ctccagtctg gtcagctcat caccatcgaa
    51301 ctaccggaat ggctgctggc gatggcgtat atggcaattg gctggcggat tggtcttggt
    51361 ttcgataaac aaatcttact gcgggcacta cgcccgctac cgcaaatcct gctgtcgatt
    51421 tttgctctgc tggctatttg tgcaggtatg gcgtggggac tgacccggtt tatgcatatt
    51481 gattttatga ctgcctacct cgccaccagt cccggcgggc ttgataccgt ggcggtcatc
    51541 gccgcaggga gcaatgccga tatggcgctt attatggcga tgcaaaccct gcgcctgttc
    51601 agtattttgc tgacggggcc agccattgcg cggtttattt caacctatgc gccgaagcgg
    51661 tcggcctagt gctggcagcc tggacaccag taaaacgggc gcgaggacaa cgtggttttc
    51721 tcaatgatgc cgccacagcg ttcgcacagt tcgccatcac gatgaaaaac cttaaagcga
    51781 aacagcgccc catgatgctt attctcatcc acctgccccc gcgtagcgta agaaaaccga
    51841 ggaatctcca gtaacgcgtg tgcgagtgca tccagttgcg ccgcattgag atctttcgtt
    51901 ttatgatttc cagtcaaccc aacctgccag aggatctcca cccgcaaata attgccaagc
    51961 ccagccagaa acgcctgatc gagcagtaat cctgcaaact gacggttacg aaagcgcggc
    52021 gacaataacc gttcctttac cacctccggc gtcagattcg gatccagcac atcgggacca
    52081 acgcgttgta aaaacggatg cgtggtcagt tgttccggcc tcaacatctc aatatccgag
    52141 gcgctataaa gcagaatggt tttgtcagcc gtttgcagtt ttacccgcaa tactcgcgtg
    52201 gtctgcggct cttcgccggt atcaaccacg cgccagacgc cgtaaagctg attatggctg
    52261 tagagcgtta aaccgttggg aaaatgagtt aacaacgcct taccacgcgt ttccacatgg
    52321 gtaacgtgtt gaccgataag ttgtgattga taagttttta actgcgggaa ggcaaaccag
    52381 acatcagtta gtggtttgcc tttgatcgcc gcctccaggt tatccgctgc acggcggatc
    52441 tccgggcctt caggcatggt gatatccttt tttcattctt tattaagcgg ggttatgctg
    52501 cgacaacaat cccctttttg gcaaatgtag ctcgtaatcg gcgggcgaac gccagagcgt
    52561 gctcgccgtc gccatgcagg cagaccgttt gcgccgcgac cgttgcccat tcgccggtga
    52621 tacttttgac tctgccgtgt tgcaccatct ccagcgtttg cgccaacgcc tgttcttcgt
    52681 tttcaatcaa cgcgcctggc tgacttcgcg gcaccagcga gccgtcagcc tgataaccgc
    52741 gatcggcaaa cacttcctcg cgcgttgtca ggccatattg cttgcctgca cgaatcagct
    52801 cgcttcctgc cagcccaacg agaaccaatg ccggatcgca agcgtatacc gctctggcga
    52861 tggcgtctgc cagttgtgct tctttcgccg cctggttgta caacatgccg tgcggtttga
    52921 catgacgcat tacgccgcct tgcgcacggg caatcgttgc cagcgcgcca atttgataca
    52981 gcgtctgggc gtaaacggtt tccggcggca gctgcatggc gctgcgacca aaattttccc
    53041 tgtcgggaaa actcgggtga gcgccaatcg cgacaccatt ttttattgct tcacgcacgc
    53101 aagcctgcat ggtttgcgca tcgcctgcat gaaatccaca agcaatattg gcagaggaaa
    53161 ccagcgttaa tagctctgcg tcgctggcgc agccttcgcc cagatcggcg ttcaggtcaa
    53221 ttttcattgt gcagccgcca cgctaattgt tcaaaataac gttgctgatc ttgccgcgct
    53281 ttcagtgcct cttccagtga acactggaca aaatgaatcg gctgaccgag cggaatttgc
    53341 gccagatggt acatatcagc ctcaatgata caggcaatac gtggataacc accggtggtc
    53401 tgtgcgtcgt tcatcaacac aatcggctgc ccgttatgcg gcacctgcac cacgcccggt
    53461 aacaaaccgt gagataacag ttcgcgatcg gtgatgcgtt ttaaaatttg cccctgtaaa
    53521 cgatagccca ttcggttact ttgcgagcta agctgccagg gcgaacgcca gaatgcatcc
    53581 tgcgaggcgc gatcgaactc atgatattcc ggccccggca aggcgcgaat gcggttgccc
    53641 cacagcagct gtttaacgcc ctgcgcttcc ataaaatcac gcttcgattt gccaatcggg
    53701 agtcagtcac catccttcag taaacggcct tccagcccgc caatccccac tttgagatcg
    53761 gtgctgcatg accccattac cggcggaaca tcaataccac ccgcgaccgc cagataactg
    53821 cgcatcccgt gctgcgggcg tttaagcgtt aaacgctggc ctgctttcat cggcaatcgc
    53881 cagccggtcc agacggcgtt atcatccaac cgcgcttcgc aacctgcacc cgtcagggca
    53941 aaccacccat cagtttcgaa ctcaactgtt aactgaccga gcgtgatctc cagcgcgggg
    54001 gcattggcgt cattacccac cagcaggtta gcaatgcgca gcgcaggcat atccagtgcg
    54061 ccacagtggc tgatacccga ctggcaaaaa ccgtgacgac cgccatcctg cacagtggtg
    54121 tacatgcccg cacgaataat cttcagcata ttccctcctt ctgcggtaca aatcgcacgc
    54181 tgtctcccgg acgtaataag atgggttcgt cacgcgccgg atcaaacagg ctgagtgagg
    54241 tatgaccaat caactgccag ccacccggcg ttgccagcgg ataaacacca gtctgcggcc
    54301 cgccgatccc gacagaacct gccggaacga gcaagcgcgg ttcagcgcgc cgtggcgtgt
    54361 gtagttgttc cggcaaactc ccgagatacg ggaagcccgg ttgaaaacct aaaaaccaga
    54421 ccacgtattc cacggaggag tgcaattcaa caacctgttt ttcgctcaac ccgcaatacg
    54481 ccgcgaccac cgccaaatcc ggtccgcctg caccaccgta aaccaccgga atttcaataa
    54541 agcgagactc aggttccagg gcctcgctct cctcccacca gcgttgcaaa cgctctatgg
    54601 catccagcgc cagcgactca ggattacgca aaatcaccgt gatattgttc atgccgggaa
    54661 tggcttcaac cacattcggc atatccacca gacgctgcgc cagtcgccag atccgtttct
    54721 ggctagccag cgtcaccggc ggttccagtt ccagcactac cgccgtttca cctatcagat
    54781 aacaacgcgc tcgttgcact ttcgatccct ctgattattc gttatgcagg attaggaata
    54841 tcaataaagg tcacatcaag atcggtattt tcattcagcc actcgctcaa tgcgcgaata
    54901 ccaccgcgtt cagtggcatg gtgacctgca gcataaaaat gcaatccctg ctcgcgggct
    54961 gaatgaatgg tctgctcaga aacttcgcca gtaataaaag catccacgcc aaaacgcgcg
    55021 gcgctatcga taaaactttg cccgccgccc gtgcaccagg cgacgcgctg gaccacctca
    55081 ggtccggtat cgccacacca taacggctta cgtcccagac gcgcttcaat ccaggaggct
    55141 agttccattc ccggcacagg catggtcagc tctccccacg gcaccaacgg ctcaatttcg
    55201 cccatgaccg tgatccccag taacgccgcc agctgtgcgt tattgcccag ctcaggatgt
    55261 gcgtcaagcg gcaaatgcca gccatacagg ttgatatcat tcgccagcaa cgtttttaaa
    55321 cggttacgct tcattccgcg aatgaccgga gactctcctt tccagaagta gccatgatgc
    55381 acaatgaccg catctgcgcc cagacgcact gcctcatcga gcaaagcctg gctggcggtg
    55441 acaccggtaa caattttttg caccgtctct ttgccttcca cctgcaaacc attcggcgcg
    55501 taatcactaa tcgccgcgct gttcagtttt tcgttaatca gttgttccag ttcggtgttt
    55561 ttcatcttcg cgccttccgt attctgatag ctacaccgtt aacatagcgt tacatcgcct
    55621 gttcgtcgat agccaatctt ttgacatatt aagaaacaaa cccgacgcca aaagacgcaa
    55681 attgtttata tcatcacgct tcagctaatg tatatttatg caatcagaca gcatattgca
    55741 taaataatca gatctgtatc aaagttttaa ctttaaggaa acacaagcac gccgcgcaac
    55801 tattacgtga cgttgccgtc aattgtggaa ttattatgaa taaacacgca tcacaaccgc
    55861 gcgctattta ctatgtcgtt gcgctgcaaa tctgggaata ttttagcttt tacggcatgc
    55921 gtgccctgct gattctctat ctcaccaatc aactaaaata caacgatact cacgcctacg
    55981 agttatttag cgcctactgt tcgctggtgt atgtcacgcc aatcctcggt ggctttttgg
    56041 cggataaagt tctcggcaat cgcatggcgg tgatgctggg ggcgttgttg atggcgatcg
    56101 gtcatgtggt gctgggtgcc agtgagatcc atccgtcatt cctctatctg tccctggcga
    56161 ttatcgtctg cggctatggc ctgtttaaat ctaacgtaag ctgtctgctc ggcgagctgt
    56221 atgagccaac cgatccgcgt cgtgatggcg gtttctcgct gatgtatgcg gcgggtaacg
    56281 tggggtctat tatcgcacct atcgcctgtg gttacgccca ggaagagtac agctgggcga
    56341 tgggctttgg cctggcggcg gttggcatga tcgcgggtct ggtcattttc ttatgtggca
    56401 atcgtcattt cactcatacc cgcagcgtta acaaaaaagt actgcgtgcg acaaactttc
    56461 tcctgccgaa ctggggatgg ctgctggttc tgctggtggc aacgcctgca ctgattaccg
    56521 tgctgttctg gaaagagtgg tcggtatacg ccttaattgt cgcgactatt attggcctgg
    56581 gtgtactggc aaaaatttat cgcaaagcag aaaaccagaa acagcggaag gagctggggc
    56641 tgattgtgac gctcaccttc ttcagtatgt tgttctgggc cttcgcacaa cagggcggta
    56701 gctcgattag cctttatatc gaccgcttcg ttaaccgcga tatgtttggt tataccgttc
    56761 cgaccgcgat gttccagtcg attaatgcct tcgcagttat gctgtgcggt gtgttcctgg
    56821 cgtgggtggt taaagaaagc gtcgcaggta atcgtaccgt gcgcatctgg ggaaaatttg
    56881 ctcttggcct tggcctgatg agcgccggat tctgcattct gaccttaagc gcccgctggt
    56941 ccgcaatgta tggtcactct tctctgccac tgatggtgtt aggcctcgcg gtgatgggct
    57001 ttgcggaact gtttatcgac ccggttgcca tgtcgcaaat tacgcgcatt gaaattccgg
    57061 gcgtgaccgg cgtattaacc ggcatctaca tgctgctttc tggcgcgatc gcgaactatc
    57121 tggcgggcgt gattgccgat cagacatcgc aggcttcgtt tgatgcttcc ggggcgatca
    57181 actactccat caatgcatat attgaagtat ttgatcaaat tacctggggc gcactggcgt
    57241 gtgtaggact ggtactgatg atttggctgt atcaggcgct gaaattcaga aaccgcgcgc
    57301 tggcgctgga gtcataatag gccagcaccg atgcctgatg cgacgcttga cgcgtcttat
    57361 caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc tggattaggc
    57421 gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc
    57481 gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt
    57541 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgctt
    57601 tccctggtac atcgcgcagc tccggtaacc atcgacgaat aaactcgccc tcacggtcaa
    57661 atttctcgcc ctgagttatc ggattgaaaa tacgaaaata cggcgcagca tcagtacccg
    57721 tagaagccgc ccactgccag ccaccgttat tagctgccaa atcaccatca atcagctgcg
    57781 acatgaaata tcgctcgcct tcgcgccagt cgatcaacaa atctttcacc agaaaactgg
    57841 ctgtaatcat ccgtagccgg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
    57901 catcaataat cgggtatccc gttttgcctt tctgccaggc ctgtaaatgt gcgggattgc
    57961 tttgccactg cacacgatcc gtccaggcaa taaatggacg atgtttacac aacgaggggt
    58021 aatacgtcat cagatgacgg tagaactcgc gccagatcag ctcactaagc cagacactac
    58081 cggccccacc gtccagcgcc tgcggctgtt ccgccaacaa gcgatgcaaa cactggcgag
    58141 gcgataaccc gcccgttgcc aagctggcgg acaaacggct ggtgccttcc actgccggaa
    58201 aatctcgttg ttgctcatac tctccagcac cgttctcgca aaactggcgt aattgcgcaa
    58261 tcgccgcttt ttcttccacc gggaaatgcg cagtatcgaa agactgacga ggataattca
    58321 gcgtaatgga tggcgcgggc tttatcgatc cgctactacg aacttttggt gcagcgacgc
    58381 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
    58441 tgtacatctc gtgattgccg gtcatcaccg cccccggcgg caggatcacg ctgtcatcaa
    58501 atccttcaca caccacgtta cgcagcgctc tttcaacgtc cacatcccgc gctcgctcat
    58561 tcacctcata ctgatagtta taaaaaagat gagtaacgct gttttccgcg cacacctgtt
    58621 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
    58681 tttccgccag cgcgatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
    58741 gcgacatgtt atgcgtctcc cactggcgtg gtgtagcgat atacagcgcc agcacgcgtg
    58801 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcctggc
    58861 gaaaccaggc cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
    58921 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca ggataaagcg ccatatagtg
    58981 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
    59041 cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
    59101 catcaaaacg ttggtgtgat cgcgcgggtt ggcgggatga gaaagcaaaa caattacgcg
    59161 ttggcggtac tggttgtaaa aatcatccag attttgccac tgatgaatat cagccacaaa
    59221 cgggccaagt ttgcggtact cgggttgaga atgggccaga aagacgagtt tatagcggct
    59281 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
    59341 aagggcgaag atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc
    59401 tgaacggtat acaggagtaa gtgtaggcgt tggtaaatga tgtttgtgaa gttaatccgc
    59461 tagcagggca agattacgaa agtgcgctcc ccgcaagcaa ctaatcaagg tagttaatca
    59521 tggctgaaca cgatattacg ccagaagtca ccatttccaa aacacagcgc cgctataaag
    59581 tcggctatgt cagagcccgc caccaggacc gcaaaaccgg tatagcccgg tattattcac
    59641 aacatcccag cctgcacctt aagggcgact ggctgaaaga ggccggattt gataccggac
    59701 ggggtgtcac ggtgaagatt tcggaggggg gcctgaccat catcgctgac agtgacgagg
    59761 tgcaggaact gcgggatggg atgtttagtg tgctgaacga aagttaaata ttaaaaaagc
    59821 tgggaagatt taaaaccttc ctagcttttt attctgtctt attataatgg atactcatca
    59881 tcttccgatg aatttataaa ttgctccaag tttacatgaa gagtacttaa cctagttttt
    59941 aactcattaa gtgaagcgat aatttcatat tttccaacat aatcatatga tggatcgtat
    60001 tcattatcta tgtcagaagc taaaaatgat gatattcgta gtttaaattt agcttcatct
    60061 ttattacatt cattggcata aggtgaatgc cctatcatag catattcatc taatctaaaa
    60121 ggataagatg gttttaattc atcgatcaat gaaatttcaa tccatgttct atatccaggc
    60181 attaatggac taatttcttt gagcaaaacc tttccactta ataatgtatt gagattgttc
    60241 attattatgt ctcccacggg aaaaatatat gtctaacact tccaccatgt tgatgaactt
    60301 gtaaatgtgg taaatcacca tcaggatttc ctggtccatg acctgacact ctatatgaag
    60361 gttttgttac agaaggatct tttatatcat cccaatgata tgaacctgct tttgttttat
    60421 ttccagtaag atattccatt aagttcttag tgctggggcc tgactcacct gtcatatttt
    60481 tataattatg acctaaatat ctatccataa acaattgttc agcgtcactt ctagacctaa
    60541 cagtaacttt ggtttcacta ctatctaata aacgattcgc tttttggtta ctaacagcac
    60601 atggagttaa tccaagtgga tcagcatatt ttagaggatt aggtgcataa ctgtagagat
    60661 ttaaaccgcc tctcaaccct atcgggtcag ttaccatgaa ccgcccgaca tccggatcat
    60721 agtacctgtg cagattgtag tgcagccccg tttccctgtc gaggtactgt ccctggtagc
    60781 gcaggttctg ctccaccccg ccgtggtcct tctcctgtat cgtgttaccc cagacctgat
    60841 accccgtctc ccacacgatt ttcccgtcgc tgtccgtcat ctcttccggc gcgccgttca
    60901 catccgtgtg aaaatacagt atccggttcg ggccctcttt gcccgcctta tccacccgcg
    60961 ccagcggttc ataactcccc tggtccctgt agatgtacag gctgcctgtt ccctccggcc
    61021 tgctctcctg cgccagccgc attccgtccc ataaaaattt tgtccggttg tacggcttac
    61081 cgtcagggct gatgtgctgt ttttccgtcc gcctccccag tgcatcgtac acataccggt
    61141 atgtcccccg cgctgtcgtc acttccacca tccggtgttc cgcatcataa cggtaactct
    61201 gcgttccgct ccggccctcc ttttccaccg cacggccaaa ttcgtcataa cggtagtgca
    61261 caccacggta agacatcagc cggttgaacg gcaccacgct gttttctgcc gtggcccttt
    61321 cccccgcgcg cctccccagc aggtttcccg ccgcgtcgta ctgccagctc gccagatacc
    61381 cctcatcctc cgaacgacac gccgtgatgc gccccgtggg gtcgtagaag taatccgttt
    61441 ccccccgccg gctgtgccgc gtcttcagca gttcatcctc gccactgtag ctgtactgcc
    61501 ggtccgtgaa cgtctccggt accaccccgc gcatcccgct gtatgtctcc ctgcgcttca
    61561 gccgcccgcc ggggtcgtaa agctgccgcg tgctcagtcg cccctggctt ctgagcactt
    61621 cacggtgcag gtggtcgcgc gtgaactcgc tgatgacctc ccggtccaga tttatctgct
    61681 ggagatggcc gctgccgtag tacagatggc gctgcgttct gccgtccgga aaggtggtgg
    61741 cagtccggtt gcccagttta tcgtactggt gggtcaggat gccctgcgcc gtctcctcgc
    61801 tcagcatccg ccccagtctg tcgtagctga agcggataac ttccggcgcg tcagtttcgt
    61861 gccgctggcg acggatttcc agtagctggt ctgccgcatc atggacgtat tccgtacggc
    61921 catcggctgt ctcttttgcc gtgagccggc ccgccgcatc acgcgccagc cggtggcgga
    61981 tttcaccgcc ccggtccccg gtgaacacca cttccgtgac cgcgttcagc gcgttgtagc
    62041 ggtacgactg gcggcagccg tccgggttta tctgctccgc cagacggtcc agcacatcat
    62101 ggcggaaccg gtagcgttct ccgttctcgt tcgtgagcgt gaccagacgc ccgtaagcgt
    62161 cgtacccgaa tgccgtcctg cgccccatcg cgtccgtccg gctgcgcacc cggccctgcc
    62221 cgtcccgctg ccagcgcgtt atcctcccca tcgggctggt gtactttacc agcctgcccg
    62281 ccgcgtctga ctggtaattc accgtgcggc cgtccgccag tatcacctcc gtcagatgac
    62341 cgctgtcgtc atacccgtag cgcgtgctgt ggctctgcgc atccgtcatt ctgataagcc
    62401 tcgtgcgttc gtcgtagaac cacgccgtct gcgagccgga acagtccgtg tgccgcatga
    62461 gctgcccgtc ttcattccaa tggaggtatt tattcccccc gcgcgcatcc gttatctgca
    62521 ccacctggcc gtggtggtca tactgatacc gggtgttctg ctgcagcggg tcggtcacgg
    62581 caagaaggtt gccgcgttca tcgtacacac agcgccaggt gtttcccgcc gcgtccacct
    62641 ccgtttccgg ctggtgccac accgggtgcc actgcgcctg ctccacccgc cccagcgggt
    62701 cgtgcgtctc cgtgatatgc ccctgcaggt catacacata gcgccatttg ccgccctgag
    62761 ggtcggtcat ccccagcaac agtcgttctt cgtcgctcca gcggaatgtc gtcacctgtc
    62821 cggcttcgtc ctggtacgcc gtcacctgat tctgcgcatc ccactggtga taacgtgtgc
    62881 gccccagtcc gtccgtcacg cacagcctgc gctgcgccag gtcccacgcc agccggtact
    62941 gttcaccgct gtccgtgtgg tgccccgtca cccgccatgc cgcgtcatcc ggaccttcat
    63001 accagcccca ctggtaataa tacccttcgc cgcccggctg gcggtgcagc gtcatgcagt
    63061 ggttttcgtc gtaagcaaac tcacgcagca ggtgccccgt gctgtcgtac acgccgttca
    63121 ggtggccggc ctcgtcatag ctgtaacgac gcagcagtaa aggtgcagtc tcagggaaat
    63181 gctgataaat ctgtgtcacc cgccggggat gcgccgccag ttcgtaatac agacggatgc
    63241 acggacgctg ctgttcgccg ctgatttcca caatgcggcc ccggtcgtcg tagtacagat
    63301 tcagacagtt gctgttacgg tcgcccagca ttttcagccg ctggcgctgc gggtggtgcg
    63361 ggtcagcctc aaacagaaaa cactgcccgt catcgctgct gataagccag tgcccctgct
    63421 caccacgcct taccgccagc ccttcaccgg ggctgtaaaa cccgcccccc ggcagtacgg
    63481 cttccatatc caagcgtcgc cccatgcccg acacaaaggt catgcagttt tcgtctgcgc
    63541 tacccggtgt gcacacgagg ctgacttcat acattacact ccagccctga ccaaacattc
    63601 cttccgtacg ttcgtcacga ctgctgtaca cgcgctgcca gtccagcgga aagtggcccg
    63661 gcagactgaa atcgaagtca tcgtcatcgg ccaggtattt ggccacggtg gcggcattga
    63721 ccggatgcgg aagggcactg gtggccttct gcgtcagcat gctcatcccg acactggcag
    63781 caaaacaggc gattttagaa agcattttgc ccgggcgaag aaacatcagc gccacggttg
    63841 ccaccagggt tatctgcgac ctgccgcttc tgatatccct gaccaccagt aacggaccgc
    63901 caatgcgcac atccggtgac acaaacgccc cgttccccgg ctcattcacc accctggcct
    63961 cgcaggtaca gcgtgccccg ctgcgtgtcg ccggctggct gttgatacgc acatcccgcg
    64021 cgccttccgc gatatattcc gctgcgggcg gctcgtcatc gcctttgaac agattactga
    64081 tgctgccaat aaccgcattc actccctgaa agacggtttc cagcataccg cccggattca
    64141 gcaggttaag ggccgttccc agggggtcat ccagaaaggc ggcttttttt tgtgcgagaa
    64201 actgcgtcag tgaggaagga tgtttatggc agtcgatttt atcgtcaggg gtcggggtgg
    64261 atgccggggc cgcactggcc acagtgggct gccatagttc cgacacaaaa tgtgtcacgc
    64321 catccaccat ttcagaacgc ccttcttccg tactgagctg gtcgtgcaga gcccgggaca
    64381 tcagcacagg ccctgtcacc gggttaaccg ccagggctat ggcccccgcc atttccagat
    64441 attccccggc cacatcccgg aacgttctgg catcctcctg acgctgcgct tcttcctgtt
    64501 ctgccttcgc ctgcgcttcc tgcgcctcta tgtcctgccg cgatgccgca gtcgccgctg
    64561 cccgcgcggc aggtttgctg ttgatccatg tgtcaccaga gccggtgagg atgtaacctt
    64621 caatctgagg cgggaaaagc atatttgcga gggctgaaca ggcttcgctg accttttctg
    64681 tcacgccggt gtacaccatc gccacacccg ccacaaaacc ggtcagcacg acggccgtgc
    64741 accccatcgt gatccccact gacgtcgcca gtaacgaggt tgtgccgaca atgagggcat
    64801 caataacgac atttgcggcc acctccagca ccgcccccag cacatctgcc ataaacgacg
    64861 tatgcagcag ggcatcaccc tgtcttgccg cccagaatgc ctcttccatg tcccgttccc
    64921 tgtatgatta attcgtcctg acttcctgcc gcatcctgaa gcgcagattc tttttaattt
    64981 cagcccagcg cgtcatatct gccggggtca gcggtgtggt tctggcgtag gtgaaggcca
    65041 tcatgacccg ctcacccggc aatacaaatg ccacctgaca ctggtacacc tgctcgttac
    65101 cccggagaaa ctggctctgt atttccaaac cgcctcgttg agataatatt ccgtcataat
    65161 gtaaatagtc cgttttaata aaggaattat ttattgacag gttctgtttt tttaatcatg
    65221 aatgtaatac ccatcaccgc tttacagcac actttatctt tccagccata atcactaata
    65281 atattaagca attagcaata tttacaacga ccagagcgga tacatagtta tgtatttaac
    65341 aatcccaata tattcactat gcaaataaaa ccactagtaa cagacacgct accgcctcca
    65401 tataaaaatg gacaccttat aaaattgtat cttgcataga aaaagctgaa atatcggatg
    65461 cgttttaatt atcaactata aatctataag ctttttatta acaacaa
//
LOCUS       DAOFMX010000010        62288 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.010, whole genome shotgun sequence.
ACCESSION   DAOFMX010000010 DAOFMX010000000
VERSION     DAOFMX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.010"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="QBA20_000779"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="QBA20_000779"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDQ6291421.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="QBA20_000780"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="QBA20_000780"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDQ6291422.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="QBA20_000781"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="QBA20_000781"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDQ6291423.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="QBA20_000782"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="QBA20_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDQ6291424.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="QBA20_000783"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="QBA20_000783"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDQ6291425.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVHALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="QBA20_000784"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="QBA20_000784"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDQ6291426.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="QBA20_000785"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="QBA20_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDQ6291427.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="QBA20_000786"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="QBA20_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDQ6291428.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="QBA20_000787"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="QBA20_000787"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDQ6291429.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="QBA20_000788"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="QBA20_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDQ6291430.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="QBA20_000789"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="QBA20_000789"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDQ6291431.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="QBA20_000790"
     CDS             complement(15665..16702)
                     /locus_tag="QBA20_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDQ6291432.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="QBA20_000791"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="QBA20_000791"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDQ6291433.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="QBA20_000792"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="QBA20_000792"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDQ6291434.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="QBA20_000793"
     CDS             19444..19884
                     /locus_tag="QBA20_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDQ6291435.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="QBA20_000794"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="QBA20_000794"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDQ6291436.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="QBA20_000795"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="QBA20_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="QBA20_000796"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="QBA20_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDQ6291437.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="QBA20_000797"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="QBA20_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDQ6291438.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="QBA20_000798"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="QBA20_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDQ6291439.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="QBA20_000799"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="QBA20_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDQ6291440.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="QBA20_000800"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="QBA20_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDQ6291441.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="QBA20_000801"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="QBA20_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDQ6291442.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="QBA20_000802"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="QBA20_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDQ6291443.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="QBA20_000803"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="QBA20_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDQ6291444.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="QBA20_000804"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="QBA20_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDQ6291445.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="QBA20_000805"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="QBA20_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDQ6291446.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="QBA20_000806"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="QBA20_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDQ6291447.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="QBA20_000807"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="QBA20_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDQ6291448.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="QBA20_000808"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="QBA20_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDQ6291449.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="QBA20_000809"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="QBA20_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDQ6291450.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="QBA20_000810"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="QBA20_000810"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDQ6291451.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="QBA20_000811"
     CDS             37412..37681
                     /locus_tag="QBA20_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDQ6291452.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="QBA20_000812"
     CDS             complement(37684..38043)
                     /locus_tag="QBA20_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDQ6291453.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="QBA20_000813"
     CDS             38184..38810
                     /locus_tag="QBA20_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDQ6291454.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="QBA20_000814"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="QBA20_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDQ6291455.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="QBA20_000815"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="QBA20_000815"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDQ6291456.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="QBA20_000816"
     CDS             41650..42315
                     /locus_tag="QBA20_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDQ6291457.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="QBA20_000817"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="QBA20_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDQ6291458.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="QBA20_000818"
     CDS             complement(42949..44166)
                     /locus_tag="QBA20_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6291459.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="QBA20_000819"
     CDS             44300..45349
                     /locus_tag="QBA20_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDQ6291460.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="QBA20_000820"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="QBA20_000820"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDQ6291461.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="QBA20_000821"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="QBA20_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDQ6291462.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGVD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="QBA20_000822"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="QBA20_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDQ6291463.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="QBA20_000823"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="QBA20_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDQ6291464.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="QBA20_000824"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="QBA20_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDQ6291465.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="QBA20_000825"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="QBA20_000825"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDQ6291466.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="QBA20_000826"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="QBA20_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDQ6291467.1"
                     /translation="MQRVTITLDDDLLKTLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="QBA20_000827"
     CDS             complement(51544..51903)
                     /locus_tag="QBA20_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDQ6291468.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="QBA20_000828"
     CDS             complement(51900..52175)
                     /locus_tag="QBA20_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDQ6291469.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="QBA20_000829"
     CDS             complement(52234..53358)
                     /locus_tag="QBA20_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6291470.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="QBA20_000830"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="QBA20_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDQ6291471.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="QBA20_000831"
     CDS             complement(56090..57157)
                     /locus_tag="QBA20_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDQ6291472.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57523..59145)
                     /locus_tag="QBA20_000832"
     CDS             complement(57523..59145)
                     /locus_tag="QBA20_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDQ6291473.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..60996)
                     /locus_tag="QBA20_000833"
     CDS             complement(59407..60996)
                     /locus_tag="QBA20_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDQ6291474.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61380..62288
                     /locus_tag="QBA20_000834"
                     /pseudo
     CDS             61380..62288
                     /locus_tag="QBA20_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
BASE COUNT        14845 a        16955 c        15598 g        14890 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgcag aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca tggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcaacacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtaccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg gagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaagatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttatgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcggg aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag cccttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat caggttaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aacagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgat aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcagaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtgca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgcc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctgaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgtagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc gcgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatcg cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 ttgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 gaagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttc cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caggcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcactt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggccggaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcctggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatt
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaagatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaat aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg
    60001 gctatcttta ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc
    60061 accaaaaata gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat
    60121 cgcatcgtcc tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt
    60181 taataaattg tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc
    60241 gtgttttaat aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga
    60301 aacgagcaat gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt
    60361 gttggcattt tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac
    60421 agatttaaca tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg
    60481 ccagtcagct ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag
    60541 cccattaact ttataaatta tgtctgattt atctccggag aaattaataa aataataacc
    60601 atccttacac tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc
    60661 tggtataatt gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg
    60721 aatatctata gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc
    60781 atttaccctc cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc
    60841 tatttgcgaa tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc
    60901 aaccttgcta tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga
    60961 agggatgtac ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc
    61021 tgttagtaaa aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat
    61081 aatacttata tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg
    61141 gaaaacaatc catccctgga agacaattgt aaaaaaaaca acagaataag aattaatctt
    61201 aggataaata tttatttatc atggcctttt gaccgctaaa aaatggcact cggcacagta
    61261 aaccccgcta tagttatgct atgcatttgt ctttaatgat atctcgtcgt tatattaaca
    61321 ggctgaaatt atcattaatg cacatttcaa tattagcagg gatacctgag gagtttaaag
    61381 tgaacatata tatcgggtag cttttcaaat taatcccttt gattataggc ttaatttgca
    61441 tcgcgttagg tggctttgtg ctggaaagtt cagggcaacg tcacgggtca tgtgctgatt
    61501 tctctggcgg ccatatgcct ggcattattc actaccgcat ttattatcat ttcgcaactc
    61561 acgcgcggcg ttaatacgtt ttacaataca ttgttcccca ttattggcta tgcggggtca
    61621 attatcacca tgatatgggg ttgggcactg ttagcaggta atgatgtgat ggcagacgag
    61681 tttgttgccg gccatgttat ttttggcgtt ggtatgattg ccgcatgtgt atcgacggtg
    61741 gcagcgtcgt ccggtcactt tctgctcatt cccaaaaatg cagcggggag caagagcgac
    61801 ggaacaccgg tacaggctta ttcttcatta atcggtaatt gcctcattgc cgttcccgtt
    61861 ttactcaccc tgctcggttt catttggtct attacgctgt tacatagtgc cgacataact
    61921 ccgcattatg tcgccttggg ttaaccgcaa tctgtgcctg tctaattggc ctagtggcca
    61981 caatcgtcca tcaaacacgt aatacgttct catccaaaga gcactggctg tggtgttatt
    62041 gggttatttt tctcggctca gtcacggtgc tgcaggggat atacgtctta gtcagttccg
    62101 atgcaagcgc ccgactggct cccggcatta ttctcatttg cctcggaatg atctgttaca
    62161 gcatattctc aaaagtctgg ctactggcac tggtctggag acgtacctgt tcgttagcca
    62221 acagaatacc gatgataccg gtatttacct gtctgttttg cttgtttttg tcatcattcc
    62281 tcgcggaa
//
LOCUS       DAOFMX010000011        60419 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.011, whole genome shotgun sequence.
ACCESSION   DAOFMX010000011 DAOFMX010000000
VERSION     DAOFMX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.011"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..322
                     /locus_tag="QBA20_000835"
     CDS             <1..322
                     /locus_tag="QBA20_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6291475.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="QBA20_000836"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="QBA20_000836"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDQ6291476.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="QBA20_000837"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="QBA20_000837"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDQ6291477.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="QBA20_000838"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="QBA20_000838"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HDQ6291478.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="QBA20_000839"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="QBA20_000839"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDQ6291479.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="QBA20_000840"
     CDS             complement(3726..4955)
                     /locus_tag="QBA20_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDQ6291480.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="QBA20_000841"
     CDS             complement(5019..5459)
                     /locus_tag="QBA20_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDQ6291481.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="QBA20_000842"
     CDS             complement(5564..6334)
                     /locus_tag="QBA20_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6291482.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="QBA20_000843"
     CDS             complement(6331..7257)
                     /locus_tag="QBA20_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6291483.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="QBA20_000844"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="QBA20_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDQ6291484.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="QBA20_000845"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="QBA20_000845"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDQ6291485.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="QBA20_000846"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="QBA20_000846"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDQ6291486.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="QBA20_000847"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="QBA20_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDQ6291487.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="QBA20_000848"
     CDS             12964..13311
                     /locus_tag="QBA20_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDQ6291488.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="QBA20_000849"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="QBA20_000849"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDQ6291489.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="QBA20_000850"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="QBA20_000850"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDQ6291490.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="QBA20_000851"
     CDS             complement(15131..15493)
                     /locus_tag="QBA20_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDQ6291491.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="QBA20_000852"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="QBA20_000852"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDQ6291492.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="QBA20_000853"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="QBA20_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="QBA20_000854"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="QBA20_000854"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDQ6291493.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="QBA20_000855"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="QBA20_000855"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDQ6291494.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="QBA20_000856"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="QBA20_000856"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDQ6291495.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="QBA20_000857"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="QBA20_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDQ6291496.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="QBA20_000858"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="QBA20_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDQ6291497.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="QBA20_000859"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="QBA20_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDQ6291498.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="QBA20_000860"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="QBA20_000860"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDQ6291499.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="QBA20_000861"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="QBA20_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDQ6291500.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="QBA20_000862"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="QBA20_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDQ6291501.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /gene="tadA"
                     /locus_tag="QBA20_000863"
                     /pseudo
     CDS             32769..33916
                     /gene="tadA"
                     /locus_tag="QBA20_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="QBA20_000864"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="QBA20_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDQ6291502.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLELKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="QBA20_000865"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="QBA20_000865"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDQ6291503.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVVEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="QBA20_000866"
     CDS             complement(36348..36386)
                     /locus_tag="QBA20_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291504.1"
                     /translation="MIIKTLKMSARK"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="QBA20_000867"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="QBA20_000867"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDQ6291505.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="QBA20_000868"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="QBA20_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDQ6291506.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="QBA20_000869"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="QBA20_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDQ6291507.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="QBA20_000870"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="QBA20_000870"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDQ6291508.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRNITLWFWLVESWEGVPWGKEGQPG
                     EWMSLIGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="QBA20_000871"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="QBA20_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDQ6291509.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIVEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="QBA20_000872"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="QBA20_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDQ6291510.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="QBA20_000873"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="QBA20_000873"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDQ6291511.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="QBA20_000874"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="QBA20_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDQ6291512.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="QBA20_000875"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="QBA20_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDQ6291513.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="QBA20_000876"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="QBA20_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDQ6291514.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="QBA20_000877"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="QBA20_000877"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDQ6291515.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRSEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="QBA20_000878"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="QBA20_000878"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDQ6291516.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="QBA20_000879"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="QBA20_000879"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDQ6291517.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="QBA20_000880"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="QBA20_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDQ6291518.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="QBA20_000881"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="QBA20_000881"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDQ6291519.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="QBA20_000882"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="QBA20_000882"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDQ6291520.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="QBA20_000883"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="QBA20_000883"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDQ6291521.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="QBA20_000884"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="QBA20_000884"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDQ6291522.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="QBA20_000885"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="QBA20_000885"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDQ6291523.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="QBA20_000886"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="QBA20_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDQ6291524.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="QBA20_000887"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="QBA20_000887"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDQ6291525.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="QBA20_000888"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="QBA20_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDQ6291526.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14479 a        16728 c        14843 g        14369 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg cccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 atcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccaag gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcaccagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accggccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc acataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ttccttgatg taaataaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggtggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagaattg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgctag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gagctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccac
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctagt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgatc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gcaccccttc ccaactttcg accagccaga
    38221 accacagagt tatattcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcgacaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgagcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcgcatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcaa
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaaa ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cttcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAOFMX010000012        60180 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.012, whole genome shotgun sequence.
ACCESSION   DAOFMX010000012 DAOFMX010000000
VERSION     DAOFMX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.012"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            414..2144
                     /locus_tag="QBA20_000889"
     CDS             414..2144
                     /locus_tag="QBA20_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDQ6291527.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            2141..3055
                     /locus_tag="QBA20_000890"
     CDS             2141..3055
                     /locus_tag="QBA20_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDQ6291528.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            3088..3336
                     /locus_tag="QBA20_000891"
     CDS             3088..3336
                     /locus_tag="QBA20_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDQ6291529.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            3336..4507
                     /locus_tag="QBA20_000892"
                     /pseudo
     CDS             3336..4507
                     /locus_tag="QBA20_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(4543..5622)
                     /gene="lptG"
                     /locus_tag="QBA20_000893"
     CDS             complement(4543..5622)
                     /gene="lptG"
                     /locus_tag="QBA20_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDQ6291530.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5619..6689)
                     /gene="lptF"
                     /locus_tag="QBA20_000894"
     CDS             complement(5619..6689)
                     /gene="lptF"
                     /locus_tag="QBA20_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDQ6291531.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(6720..7280)
                     /locus_tag="QBA20_000895"
     CDS             complement(6720..7280)
                     /locus_tag="QBA20_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDQ6291532.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(7292..8116)
                     /locus_tag="QBA20_000896"
     CDS             complement(7292..8116)
                     /locus_tag="QBA20_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291533.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(8116..9465)
                     /locus_tag="QBA20_000897"
     CDS             complement(8116..9465)
                     /locus_tag="QBA20_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDQ6291534.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9511..10269)
                     /locus_tag="QBA20_000898"
     CDS             complement(9511..10269)
                     /locus_tag="QBA20_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291535.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(10301..11014)
                     /locus_tag="QBA20_000899"
     CDS             complement(10301..11014)
                     /locus_tag="QBA20_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDQ6291536.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            11188..11880
                     /locus_tag="QBA20_000900"
     CDS             11188..11880
                     /locus_tag="QBA20_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDQ6291537.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11929..13428)
                     /gene="pitB"
                     /locus_tag="QBA20_000901"
     CDS             complement(11929..13428)
                     /gene="pitB"
                     /locus_tag="QBA20_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDQ6291538.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(13720..15579)
                     /gene="gss"
                     /locus_tag="QBA20_000902"
     CDS             complement(13720..15579)
                     /gene="gss"
                     /locus_tag="QBA20_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDQ6291539.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSSRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            15784..16650
                     /gene="yghU"
                     /locus_tag="QBA20_000903"
     CDS             15784..16650
                     /gene="yghU"
                     /locus_tag="QBA20_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDQ6291540.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16773..17021)
                     /gene="hybG"
                     /locus_tag="QBA20_000904"
     CDS             complement(16773..17021)
                     /gene="hybG"
                     /locus_tag="QBA20_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDQ6291541.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(17155..17496)
                     /gene="hypA"
                     /locus_tag="QBA20_000905"
     CDS             complement(17155..17496)
                     /gene="hypA"
                     /locus_tag="QBA20_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDQ6291542.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(17489..17977)
                     /gene="hybE"
                     /locus_tag="QBA20_000906"
     CDS             complement(17489..17977)
                     /gene="hybE"
                     /locus_tag="QBA20_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDQ6291543.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRLMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(17970..18464)
                     /locus_tag="QBA20_000907"
     CDS             complement(17970..18464)
                     /locus_tag="QBA20_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDQ6291544.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(18464..20167)
                     /gene="hybC"
                     /locus_tag="QBA20_000908"
     CDS             complement(18464..20167)
                     /gene="hybC"
                     /locus_tag="QBA20_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDQ6291545.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(20164..21342)
                     /gene="hybB"
                     /locus_tag="QBA20_000909"
     CDS             complement(20164..21342)
                     /gene="hybB"
                     /locus_tag="QBA20_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDQ6291546.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(21332..22318)
                     /gene="hybA"
                     /locus_tag="QBA20_000910"
     CDS             complement(21332..22318)
                     /gene="hybA"
                     /locus_tag="QBA20_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDQ6291547.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(22321..23439)
                     /gene="hybO"
                     /locus_tag="QBA20_000911"
     CDS             complement(22321..23439)
                     /gene="hybO"
                     /locus_tag="QBA20_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDQ6291548.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23628..23915)
                     /locus_tag="QBA20_000912"
     CDS             complement(23628..23915)
                     /locus_tag="QBA20_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDQ6291549.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(24034..24921)
                     /locus_tag="QBA20_000913"
     CDS             complement(24034..24921)
                     /locus_tag="QBA20_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDQ6291550.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            25078..26118
                     /gene="gpr"
                     /locus_tag="QBA20_000914"
     CDS             25078..26118
                     /gene="gpr"
                     /locus_tag="QBA20_000914"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDQ6291551.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(26158..26652)
                     /locus_tag="QBA20_000915"
     CDS             complement(26158..26652)
                     /locus_tag="QBA20_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDQ6291552.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26843..27727
                     /gene="yghA"
                     /locus_tag="QBA20_000916"
     CDS             26843..27727
                     /gene="yghA"
                     /locus_tag="QBA20_000916"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDQ6291553.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27999..28424)
                     /gene="exbD"
                     /locus_tag="QBA20_000917"
     CDS             complement(27999..28424)
                     /gene="exbD"
                     /locus_tag="QBA20_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDQ6291554.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(28431..29165)
                     /gene="exbB"
                     /locus_tag="QBA20_000918"
     CDS             complement(28431..29165)
                     /gene="exbB"
                     /locus_tag="QBA20_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDQ6291555.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            29417..30604
                     /gene="metC"
                     /locus_tag="QBA20_000919"
     CDS             29417..30604
                     /gene="metC"
                     /locus_tag="QBA20_000919"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDQ6291556.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            30744..31403
                     /locus_tag="QBA20_000920"
     CDS             30744..31403
                     /locus_tag="QBA20_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ6291557.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(31443..32342)
                     /gene="yqhC"
                     /locus_tag="QBA20_000921"
     CDS             complement(31443..32342)
                     /gene="yqhC"
                     /locus_tag="QBA20_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDQ6291558.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            32536..33699
                     /gene="yqhD"
                     /locus_tag="QBA20_000922"
     CDS             32536..33699
                     /gene="yqhD"
                     /locus_tag="QBA20_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDQ6291559.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33804..34631
                     /gene="dkgA"
                     /locus_tag="QBA20_000923"
     CDS             33804..34631
                     /gene="dkgA"
                     /locus_tag="QBA20_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDQ6291560.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            34831..35757
                     /locus_tag="QBA20_000924"
     CDS             34831..35757
                     /locus_tag="QBA20_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDQ6291561.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35808..36065
                     /gene="yqhH"
                     /locus_tag="QBA20_000925"
     CDS             35808..36065
                     /gene="yqhH"
                     /locus_tag="QBA20_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDQ6291562.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(36108..38327)
                     /locus_tag="QBA20_000926"
     CDS             complement(36108..38327)
                     /locus_tag="QBA20_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDQ6291563.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPEILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(38438..39850)
                     /gene="ftsP"
                     /locus_tag="QBA20_000927"
     CDS             complement(38438..39850)
                     /gene="ftsP"
                     /locus_tag="QBA20_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDQ6291564.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKDDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(39925..40662)
                     /gene="plsC"
                     /locus_tag="QBA20_000928"
                     /gene_synonym="parF"
     CDS             complement(39925..40662)
                     /gene="plsC"
                     /locus_tag="QBA20_000928"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDQ6291565.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(40896..43154)
                     /gene="parC"
                     /locus_tag="QBA20_000929"
     CDS             complement(40896..43154)
                     /gene="parC"
                     /locus_tag="QBA20_000929"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDQ6291566.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(43700..44182)
                     /locus_tag="QBA20_000930"
     CDS             complement(43700..44182)
                     /locus_tag="QBA20_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDQ6291567.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(44235..44627)
                     /gene="ygiW"
                     /locus_tag="QBA20_000931"
     CDS             complement(44235..44627)
                     /gene="ygiW"
                     /locus_tag="QBA20_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDQ6291568.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            44779..45438
                     /gene="qseB"
                     /locus_tag="QBA20_000932"
     CDS             44779..45438
                     /gene="qseB"
                     /locus_tag="QBA20_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDQ6291569.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            45435..46709
                     /gene="qseC"
                     /locus_tag="QBA20_000933"
     CDS             45435..46709
                     /gene="qseC"
                     /locus_tag="QBA20_000933"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDQ6291570.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(46755..47087)
                     /locus_tag="QBA20_000934"
     CDS             complement(46755..47087)
                     /locus_tag="QBA20_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HDQ6291571.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            47406..47987
                     /gene="mdaB"
                     /locus_tag="QBA20_000935"
     CDS             47406..47987
                     /gene="mdaB"
                     /locus_tag="QBA20_000935"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDQ6291572.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            48018..48332
                     /locus_tag="QBA20_000936"
     CDS             48018..48332
                     /locus_tag="QBA20_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDQ6291573.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(48380..50272)
                     /gene="parE"
                     /locus_tag="QBA20_000937"
     CDS             complement(48380..50272)
                     /gene="parE"
                     /locus_tag="QBA20_000937"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDQ6291574.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKSVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(50301..50882)
                     /gene="yqiA"
                     /locus_tag="QBA20_000938"
     CDS             complement(50301..50882)
                     /gene="yqiA"
                     /locus_tag="QBA20_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDQ6291575.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(50882..51709)
                     /gene="cpdA"
                     /locus_tag="QBA20_000939"
     CDS             complement(50882..51709)
                     /gene="cpdA"
                     /locus_tag="QBA20_000939"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDQ6291576.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(51734..52156)
                     /locus_tag="QBA20_000940"
     CDS             complement(51734..52156)
                     /locus_tag="QBA20_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDQ6291577.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(52157..52786)
                     /gene="nudF"
                     /locus_tag="QBA20_000941"
     CDS             complement(52157..52786)
                     /gene="nudF"
                     /locus_tag="QBA20_000941"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDQ6291578.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            52991..54472
                     /gene="tolC"
                     /locus_tag="QBA20_000942"
     CDS             52991..54472
                     /gene="tolC"
                     /locus_tag="QBA20_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDQ6291579.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            54620..55291
                     /locus_tag="QBA20_000943"
     CDS             54620..55291
                     /locus_tag="QBA20_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDQ6291580.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            55297..56457
                     /locus_tag="QBA20_000944"
     CDS             55297..56457
                     /locus_tag="QBA20_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDQ6291581.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(56495..57283)
                     /gene="ygiD"
                     /locus_tag="QBA20_000945"
     CDS             complement(56495..57283)
                     /gene="ygiD"
                     /locus_tag="QBA20_000945"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDQ6291582.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            57426..58199
                     /gene="zupT"
                     /locus_tag="QBA20_000946"
     CDS             57426..58199
                     /gene="zupT"
                     /locus_tag="QBA20_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDQ6291583.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(58257..58427)
                     /locus_tag="QBA20_000947"
     CDS             complement(58257..58427)
                     /locus_tag="QBA20_000947"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291584.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(58688..59341)
                     /gene="ribB"
                     /locus_tag="QBA20_000948"
     CDS             complement(58688..59341)
                     /gene="ribB"
                     /locus_tag="QBA20_000948"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDQ6291585.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(59445..59593)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            59715..60005
                     /gene="ubiK"
                     /locus_tag="QBA20_000949"
     CDS             59715..60005
                     /gene="ubiK"
                     /locus_tag="QBA20_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDQ6291586.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        15109 a        15928 c        14879 g        14264 t
ORIGIN      
        1 catttttatt aattactttt tcaattttaa tcatcagcag atcgcattcc atatctctgt
       61 ggataacaaa taattaatgg tgaaaaacag gttgcaacag caacgagcgg catcttaata
      121 atcaactcaa tgaatacaaa cgacgaataa atagtattaa ttaatgatta tgataaggtc
      181 agaggctaca cagaaacgcc tcaatgcctt ttgtcaattt aaggcgaccg gacaaggctt
      241 cttcttaata tattcataaa ttaaatatca ttcataataa aaacaaaaaa cttaaactca
      301 ttatttaaat caatgttcat tttcacaccg atgaaaatac tatctcattt atcttttatg
      361 cctatataat ccaccgcatt attgtcattt tatttttgat atccaatttt ttcgtggtgt
      421 atatgtctaa taaaatcttt acgcattccc tacctatgcg ctatgccgat tttccaacgc
      481 tggttgatgc tttggactac gccgctctga gtagagccgg aatgaatttt tatgacagac
      541 gttgccaact tgaagatcaa ctggaatatc agacattaaa aacgcgtgcc gaagctggtg
      601 cgaagcggtt gttatcgctg aacctgaaaa aaggcgaacg cgtggcgctg attgccgaaa
      661 cgagtagcgg gtttgtagag gctttttttg cctgtcagta tgctggctta gtcgccgtcc
      721 cgttggcgat tccaatgggc gtcggtcagc gggattcctg gagcgccaaa ctccagggtt
      781 tactggcaag ttgccagccc gcagccatta tcactggtga tgagtggttg ccactggtca
      841 atgccgcgac gcataacaac cccgaattac atgttttaag ccacgcttgg tttaaggcat
      901 taccggaagc cgatgttgcg ctccagcgtc cagttccgaa cgatatcgcc tacctccagt
      961 acacctccgg cagcacccgt tttccccgtg gcgtcattat cacccatcgc gaagtgatgg
     1021 ctaatctacg tgctataagc cacgatggca ttaaattacg ccctagcgac cgctgcgtct
     1081 cctggcagcc tttctaccat gatatgggac tggtcggctt tctcctgccc cccgtcgcca
     1141 cgcaactttc agtagattat ttgcgcactc aggattttgc catgcgtcct ttgcaatggc
     1201 ttaaattgat cagtaaaaat cgcggcaccg tttccgttgc gccgccgttt ggctatgaat
     1261 tgtgccaacg ccgcgtgaat gaaaaagatc tcgctgaact ggatctttcc tgctggcgcg
     1321 tcgctggtat tggtgctgag ccgatctccg cagaacaact ccatcaattc gctgaatgtt
     1381 tccgtcaggt taactttgac aataaaactt tcatgccgtg ctacggactg gcagaaaatg
     1441 cgctggctgt cagcttctct gatgaagcct ccggggttgt ggttaacgaa gtggatcgcg
     1501 acatcctcga atatcagggt aaagccgtcg cgccgggtgc agagacacgc gccgtatcga
     1561 ctttcgtcaa ctgcggcaaa gcgttgccgg aacatggtat tgaaatccgc aatgaagcag
     1621 gtatgccggt cgcggaacgt gtggtaggcc atatttgcat ctccggtccc agtctgatga
     1681 gcggttactt tggcgaccag gtttcgcaag acgagattgc cgcgacgggc tggttagaca
     1741 ccggcgacct cggttatctg ctggacggtt atctgtatgt caccggacgc attaaagatc
     1801 tgattattat tcgtggccgt aatatctggc cgcaggatat tgaatatata gcggaacagg
     1861 aaccggaaat tcattctggc gatgcgattg cttttgttac cgcccaggaa aaaatcattt
     1921 tgcagatcca gtgtcggatc agcgacgaag aacgtcgcgg gcagcttatc cacgcgctgg
     1981 cggcacggat ccaaagcgaa tttggcgtga ccgcggctat cgatctgtta ccgccccaca
     2041 gtattccccg aacgtcctcc ggcaagcctg cccgtgcgga agcgaaaaaa cgttatcaga
     2101 aggcttatgc tgccagtctt aatgtgcagg aatccctggc ataaatcaaa ctgtcgcggt
     2161 gacgggcgct accgggttta tcggtaaata tattatcgat aacctgctcg cccgcggctt
     2221 tcacgttcgc gcattgacgc gtactgcccg cgctcacgtc aatgataatc tcacctgggt
     2281 gcgcggttcg ctggaagata ctcattcact tagcgagctg gttgccggag ccagcgtagt
     2341 ggtccattgc gccgggcaag tgcgcgggca caaagaagag attttcaccc gctgtaacgt
     2401 tgacggcagc ctgcgcctga tgcaagcagc aaaagagagc ggcttttgcc aacgttttct
     2461 gtttatctct tcgctggcgg cgcgccatcc cgagctctcc tggtatgcaa aatccaaata
     2521 cgtcgccgag caacgactgg cagcaatggc ggacgaaatt acgctgggcg tttttcgccc
     2581 gacagccgtg tatggtcccg gcgataaaga gttaaaaccg ctgtttgact ggatgctacg
     2641 cggcctgctg ccacgacttg gtgcgccaga tacacaactc tcttttcttc acgtcaccga
     2701 ttttgcgcaa gcagtgggtc agtggttaag cgccgaaact gtacagacgc aaacctatga
     2761 attatgcgat ggcgtcgccg gtggctatga ctggcaacgc gtacgacagc ttgccgccaa
     2821 agcccgttgt ggttccgtgc gaatggttga cattcctctg ccggtactca caggccttgc
     2881 ggatatcagt accgcgttga gtcgcctggc aggtaaagaa cctatgctga cccgctcgaa
     2941 aattcgtgaa ttaacccacg ccgactggtc ggcaagtaat aaccgtattt ctgaagatat
     3001 caattggttt cccgggatta gcctggaaca cgcattacgc aacgggctat tttgattgtc
     3061 ttgttttaaa ggtgtgttaa cgtttctatg gtaaatcgtg aaatagtaat ggattacatc
     3121 ctttcctgtt tacaggattt ggtcgaaaac ggtgtggaaa ttaagcccga cagcgatctc
     3181 gtcaacgatc ttggcctgga gtcgatcaaa gtgatggatc tcttaatgat gctggaagac
     3241 agattcgata tttccattcc tattaatatt ctgctggatg tcaaaacccc tgcgcaatta
     3301 atggaaactc tacttccatg gctggagaat aaataatggg gctatacgat aaatatgcgc
     3361 gccttgctgg cgagcgtctg caatttagtg acaacgggtt aacaccgttt ggcacctgta
     3421 ttgatgaagt gtattccgcc accgaaggcc ggatcggcaa taaaaaagtg attctggccg
     3481 ggaccaataa ttatctcggt ttaaccttta atcatgatgc catcagcgaa ggtcaggccg
     3541 cgttagcggc ccaaggcacc ggcaccaccg gttctcgtat ggcaaacggc agttatgcgc
     3601 cgcacctggc gttagagaaa gagattgccg agtttttcaa tctccctacc gctatcgttt
     3661 ttcaaccggt tataccgcta atttaggcgt catcagcgca cttgccgacc ataacgccgt
     3721 ggtgttactg gatgccgaca gccacgccag tatttatgac gcctgttctc tcggtggcgc
     3781 ggaaattatt cgtttccgcc ataacgatgc taaagatctg gaacggcgca tggttcgcct
     3841 cggtgaacgg gcgaaagagg cgattatcat cgttgagggc atctacagca tgctcggtga
     3901 cgttgcgccg ctggcggaaa ttgttgatat caaacgtcgt ctcggtggtt atttgattgt
     3961 cgatgaagcg cattcgtttg gcgtgttagg cgcaacgggg cgcggactgg ctgaagctgt
     4021 cggcgttgaa gatgatgtgg atattatcgt tggtactttc agtaaaagtc tggcctcgat
     4081 tggcggtttt gctgtcggtt cggaagcgat ggaagtgctg cgctacggca gccgcccgta
     4141 tatttttacc gcctctcctt ctccgtcctg cattgcgacc gtgcgctcgt cgttaagaac
     4201 tattgccaca caaccagagc tgcggcaaaa gctgatggat aacgccaacc atctgtatga
     4261 cggtctgcaa aaactgggtt atgagttaag ttcacatatt agcccggtag tgccagtaat
     4321 aatcggttcg aaagaggaag gtttacgcat ctggcgcgag cttatctcgc cgggcgtgta
     4381 tgtgaatctt atcctgccgc ctgccgcgcc tgcaggcatc acgctgctgc gctgtagcgt
     4441 aaatgctgcc cacagtcgtg agcaaatcga tgccatcatt caggcatttg ctactctgaa
     4501 gcagtaatgt gagcccgccg tggtgtttgc gccacggcgg gtttaatgtt gtctgtcata
     4561 ccaatacacc aggctcaacg ccaccaacac aaacgctacc ggtaaaccga gcgcggtaac
     4621 aggtgcgctt aacgcaaata gcaatcccag attaaccatg atttgatagc tgatccaggt
     4681 gagtaagccc acgatgacgc ctacagcgag acggctaccc atcccaggcg aacgcggggc
     4741 actaaaggta aacggcacag ccagcaaaat catcgccagg gtcaggatgg gttgccccag
     4801 tttttcccac agtgcaaggc ggtattcgct gctgggttgc ccggtttcct gcaaataatg
     4861 gatgtaatga ttaagctgac ggacagaaaa agtattcccc ggcattgata actcttcaag
     4921 attcatactg gtgaaggccg attgccaggc aagattatct aatgtttcca gcgtttcttt
     4981 gccattcagc cattttttat gattcacacc atgcaggacc cacgttttgt cgtcttcaat
     5041 ggtggcactg cgtgcataaa tgtaggattc aagcgagaga tcgtcgcgat aatgaaatat
     5101 ctccacgccc accggctggc cctgctcgtt cagcgatttc accgtcacaa attcattacc
     5161 acgcctggcc cacagcatat tgccggtaat gtcatcgtcc tcccccaacg cggtggcggt
     5221 cgatttgatt tgcaacgcct gttgctgcaa tggcgacgcc acccactcat ctatcgcgcc
     5281 taacgaaaca gtccacaata tcccggcaac cagcgccacc agtgcaatac ggaagataga
     5341 aaaccccgtg ctgcgaatgg cggtaagctc actgtttttc gataactgcc ccagtccaac
     5401 aataccaccc aataacgcga taaacggcga aagttcgacc agtgtgcgcg gtaaggtcat
     5461 tagcaccacc agcaccgcct gagtccagcg ataaccgccc gggctgacac catccagttc
     5521 gttaatcagg ttgaaggtgg taaaaagcgg cagcaatagc cctgcagcgg cggcaaaacc
     5581 gagaaagaga tgacggatta aatagcgact gaaaacattc atcgggaaaa tttctgcagc
     5641 aaagagaagt cacgtgcgac cagtatcagc aggcccatta gcatgagtcc gggcactaac
     5701 cacaaaccag gaatgagggg aatcgcgccg ttagccacca gtgtacggca gacgtcgccg
     5761 ccataaaaaa tggcaataaa cagcaacgtc agtggcaata acgttgaaaa tcgcccttgc
     5821 cgcggtttta cccgacttaa tgaaatggcc agcagcgcca tcaacaatgc ggtcaggcca
     5881 cggctttgtc gccattgcag ttcggcatga tcggcaggaa aggcggagcg cgccagctcc
     5941 gtgaccgatt tcgctttgcg tttgacgttc gggctttgat ccagcggatt cagatgtaat
     6001 tgcagattac ggtagatctg ttcgttgtcg tcacgcccct gatgatcgag aagataggca
     6061 gtgccgttgt gcaacataac ggtcggtttc tcgggtgatg ggtcaaccac atcaaccgaa
     6121 ccggcgcgga aaatacgggt tcgattggca gtagaagtat aaatcagcgc gtcagtcagg
     6181 cgattattat cctgatcaac cgtctgcgaa aggatcattc gtccgttatc gttagtgtta
     6241 aatttctttg cccgcaactg gcgaacatcc agctccgact gcgactgctg ctccagttga
     6301 taaatctgcg cataagccca cggacgacca tacatcgaca gaagggtgac aaaaatactt
     6361 agcggtatcg ccagataaag aacggctttg tacaagcgtc ccggactgcc ccccgccgca
     6421 gagatagcgg taatttccga gtcggtatac atctgcccta gcgtcacgcc aactgacaca
     6481 tacagaccaa caggtaacaa catctccagt gcaatcagta ctttgtagaa aacgatatcc
     6541 agcacaacat caagagctaa tgtcccattt gccgcttcgg tcagataacg ctgtgcggag
     6601 tagctggcga aaataaagat cagaaaaccg acgataatca gcaccagacg gcgcgtacca
     6661 cgcatgatgt agtgttcaac cagcttcatc tcactaacgc cctcaatcct ttgagttttt
     6721 tattcattga tcagtttctg cgccaacgca atgacatgat ctacctcttc cggcgtcagg
     6781 ctgccgtcct ttgcccactg aatttgcccg gctttattca gcaccagaat ggttgaactc
     6841 tgctccggca agcgccaggc aacgcgtccc tgtccattgc cgtcaataac gaattgcgcc
     6901 cacggatagc ggcgtttatt tttttcgatt ttgccaacaa caaaatagcc agtaccaaag
     6961 atggcatcat cggtgttcac aatggtggtg ggctgaaaac gatcctgcgg aaagtttgcc
     7021 gcttcaaccg ctttaattaa cagtgagttc tttttcttcg ccgacttacg cccggcgata
     7081 tactgaataa tgcggacttt tcccgctaat tgtgtgctgt tccacttttg gtagtgaatt
     7141 tcgtcgtcgc tattcagaag cagctcgccg ccttcctgga tataaacagg ggtaaccgtt
     7201 ttgccagtga cgaaattgtg tgcctgcaca cccggggcta agagcatgat aatcaacgca
     7261 ataatcaaac gagatgacat cattgctccg cctatagcgg aaaacgcgaa atcagcagct
     7321 ctaagcgctg acaaatatcg ctatccagcg ccaacggtgc ataatacgac tggcggtttt
     7381 ccatatcatc cgccaccatg tcgtcgtgtt tatctcccat caggccatta atggcggcga
     7441 ggaaacggga aatctgtaac gcctctttat tttcaggatc aagcgatctt aaggtgtcgt
     7501 aataagaaac ctgctggctg atgatttttc gcacatcatg aaattccctg ccggttagtc
     7561 gcggtttggc aataatctct ttcagggtat gcatttgctt ttgccgatag gcaataaaac
     7621 tgcttacgct ttctaactgg ctatggcgta atgaatagtt caccaacgac cagaccggag
     7681 agcttaaata aatccgtaac aggttgccgt aatacgacac aatatttttc ttaccgttac
     7741 gaaagccgtc ctgaaaacgc cccagaaaaa cggtcgtttt gccgaataaa aatccggcct
     7801 gatgcttctt taaatacagc cgttgcgcaa aacgcaggtg cttgtagcgc gctcgcatgt
     7861 atttaaattg tttgcgctca tcagcagtca gctcatcgcc tttggcttgt ttgcgaacta
     7921 aacgcagtaa ttcctgacga tcaacgccat tgagccagaa ttgcaggcat aacgcgtaat
     7981 tatcatgaat gatttcggtg gtacaactta gtgcaatagt cggcggcaaa ctaacctgtg
     8041 catcaacgac atcatcttct tcaatcgcaa caaatagacg ctcgctcgcc tcaagcggga
     8101 aatattttct catcatcagg atttctctcc ggctgcgtta taaagcaagg aatggcgctg
     8161 aaaaaatgcc tttaaggttg gaattaacga gagcgccacg tccagacatt gcccaccgaa
     8221 gtaagcaaac gctgctccca gcacaccaaa cagatgacag aacatgaaca gcaatacgat
     8281 gtagccgatt gaggcgatgg tttgcgccac gagaaaagcg cgctgttttc ccgccatcaa
     8341 tagcaatgac tcctgcggga agcccagcat cgaaatcaca attgcgccca gcatcacctg
     8401 aatcaggtca tacgcttcga gatacttaac gccaaacacc agcgaaatga gcggcttgcc
     8461 gacgatcaac accgcgagcg ccaccagaat accgattccg cccgccagta aaccggactt
     8521 cacacctaac aaccacggtc tggtggtgcg cggatctaaa cgcatcacct ctgggtaaaa
     8581 acttttaccc agcaaacccg ctggcgttcc ggcggcgtcg aagaatgtca tggcgatttt
     8641 aaataacccg gcggcagcgg gtcctaacac gatccccacc aacactgtgc tgcacgagtt
     8701 acgcgccgac cagatggagt gggcaatgtt ggttgaccag acaaaactcc acgcgccttt
     8761 aatgtgtcgg gcagactcaa acagattcaa tttgaaggcg ttatggatat ttctacggcg
     8821 taattcgcgc gcggcaaacc accagtacat ggtgccgcca accagattcg acacgtacca
     8881 ggcaataaca aaacccgcaa aaccaaagtc aaaataccag gctgcgacgc tccccgctgc
     8941 gcgcagaaaa ggtttcgtcg cctgctgtac agcaatcaaa tcaaagcgat ctaccgcacg
     9001 cagaatgccg gtcggcgtgg aggaagccat tgaaggaatg agcgtgcaat acagcgccgc
     9061 cagccaaaag ctttggtcat ccagacctaa tgaatgggaa aggaatggca gtaaggcaat
     9121 gccgccgaca atcgccaccg cgccgctgac gatatccagc gagaaggaaa atgagacgac
     9181 attgcgaaat tgctgcggat tattgttggt caaggctggc gttccgtact gaaccaccag
     9241 ctgccatgtc tgaaacttaa taaaatcgct gatcgacttg gcgtacgatt gcacaatcac
     9301 cagtacacca aacatggcgg gcgtcatccc tttaccggca cacgagagcg ccaacagccc
     9361 cagcaaggcg ctctcgacat tactggagcc taaccaggcg ctattgcgaa taatggtgcg
     9421 aaacgcgcca tctgcaaacc aatgtttgat gttaaaaccc gccaattcag cctgacctta
     9481 tcgttgatag taaaaaatat cccgccagcc ttaagttaaa cttcggcggt cagaaacgat
     9541 ggcaaccaga gaaaccgcct tctgtacctg ttccagcact tcgctgtagg gcgctctgga
     9601 atcaatctca agaatttttg tgccgttata gccaatcgtc gacatgacgc cgattttgtc
     9661 ctgcagctcg gcatactcat ggtcaggctt gcgggagatg gcagtatcaa tatcaatccc
     9721 caaacgaatg attaattcag gacggtattg cgccattttt tggtataaac tccgttcgcg
     9781 ctgcgccaga aacatgctga ttttcccggt cgcacgttcg acgccgatcc ccggtccgtc
     9841 ataataaaag cccgaaattt cagcctgcgg gaagcgatcg ctgaccacca gaacgccact
     9901 ttgcgccagt cgctgaacct tgcgtagatt cgccattctt cgcagcgaga agcagtacat
     9961 aatcaccgcc gcccatagcg ccggagattt ggttttcatg ctttgggttt tcgaggattt
    10021 ggccgccaat cgccgttcca gccagacgcc aaccaacggc aatcgtttga ttttgtcgcc
    10081 gtcttcgccg gagagcagcc ccagatagcg gcgctcggtt tgccagtgtt gttgcagcga
    10141 tttcaccagg tcggtggtca gcgtggattt accggtgcca tcacacccga ccaccgcaat
    10201 cagccccgga atgtagttgg gctgcggcgc ggttgtacta ttaacagttt gtgtttgtag
    10261 tgcatccatt cgttaaatca tcccctatcc attttggtga tcaggaaagc gcggcgtgaa
    10321 ttgcgcgtag tgacgcttgc ataatttcat cggctggttg ccgaccatcc agttcaagga
    10381 tcttggcgcc attgaatgtc agttgcggcg taacggcgat tttttcctgt aacgctgcca
    10441 gttggtggtc aggtttacgc gcaaacgcgg tttgttcatc aatgccaaga cgaatcaaca
    10501 atacgggtaa ataagacgcc atccattggt acagcttcaa ctcgcgctgc cttaacattt
    10561 ttatccaacc gttaccgccc gtggtttttg ccaattgcgg gccatcaaag cgaaaacccg
    10621 gcacttcaac ctgcgggtag cggtcggtga tgagcagata gccttgctgg cttttacaca
    10681 acattttgcg aaacttgtac gcccgccagc aggaaagcag atagataacc agcgcagtaa
    10741 tattgccagg cggagttaag ggcttttcgt gtacatgtgc cgctttactt cgcagataac
    10801 acccacaagg tgcgccaata acagggagct gtgaaatcca ttcgccaatt cgcccggacg
    10861 attgcccgag ataaatgtgt tctgttggca ttcttgctgc cagttcattt accaggcttg
    10921 ccgtgagggt cgatttacct gaaccatcac atcctacaat ggcaataaca cgcactggcg
    10981 tggaattaat tattgacata ttacgttgat tcacgaaaaa cccggcagta aataatgtat
    11041 tgaatattac gtggtccgtg ctcagaatat ccgttcaacc ttattgtgac aatggctaat
    11101 tatgacattg acctgaatta cgctttcata aaaacatatt aaccaaataa atatttttaa
    11161 tggatattta aattaaagga tatattcatg cagtcaataa cacctccatt aattgccgtt
    11221 attggtagcg atggttcagg caagtcaacg gtgtgtgaac atcttattac cgttgtcgaa
    11281 aaatatggtg ctgccgaaag agttcattta ggaaaacagg ccggaaatgt cggtcgtgca
    11341 gtgacaaaat tacctttgat gggaaaatcc ttacataaaa caattgaacg aaatcaggtg
    11401 aaaacagcaa aaaaattgcc tggaccagtt ccggcgctgg taattacagc gtttgtcgcc
    11461 cgtcgcttac tgcgctttcg tcatatgctt gcctgtcgtc gtcgcgggtt aattgttcta
    11521 accgaccgtt atcctcagga ccaaattcct ggcgcttacg atggtacggt gttcccacct
    11581 aacgttgaag gtggtcgttt tgtctcatgg ctggcaagcc aggaacgtaa agcgtttcac
    11641 tggatggcga gccataagcc tgatctggtc atcaaactca atgttgacct tgaagttgcc
    11701 tgtgcacgta aacccgacca taaacgggaa tcgctggcga ggaagattgc cataacgcca
    11761 cagttaacct ttggtggtgc acaactggtt gatatcgatg ccaatcagcc actggaacag
    11821 gtgttggttg atgcagaaaa agcgattacg gattttatga ccgcgcgtgg ttatcactag
    11881 tcaaaaatgg aaatgcccga tcgccaggac cgggcatttt caggaaggtt aaatcaactg
    11941 caatgctatc cagtacagcc caccagaaag aaaaattgcc gccggtaaag taaacaccca
    12001 cgccatcagg atgctggtta ccgttttacg ctgtaaccca ccgccgtcca ccaccatcgt
    12061 ccctgctacc gcagacgaga ggacgtgtgt tgtggagaca ggcatcccaa tataactggc
    12121 aaggccgata gacactgccg ccgtcatttg tgccgccatg ccttgcgcat acgtcatgcc
    12181 gcgcttacca atcttctcac cgatggtcat cgctacacga cgccagccaa tcatggtgcc
    12241 aatgccgagc gccagtgcta ccgccatgat gatccacacc ggagcgtact caatggtgct
    12301 taacatatcg ctgcgcagtt ttttcagcag gttctggtct tctttactga cgcctggcag
    12361 tttcgccagc ttcgcggagg tatcggagat gcacagcata atgcggcgca gctggctgcg
    12421 ctgactcacg cttaacggct cgtaactttc catattgcct ggcagcatcg ttttaacgcg
    12481 cgcaatcgca tcaaaggtat ttgccggatg acagtgaaac tctgttactt gcgtgccatc
    12541 agtcgatgct gcaggcaatg gaggttccat cgcaatcaac ttctgcggca gttcaggatg
    12601 ctgttgcagg tagtgttcga agttggtaac ggcatcgcgg gtacgggtaa tttcatagcc
    12661 ggacgcattc atattgacga cgaagccagc aggggcaatc cccaccagta ccagcattac
    12721 caggccgatc cctttctgcc cgtcgttcgc gccgtgcgaa aacgccacgc ccgcagcgga
    12781 aacaatcagc gcaatacgcg tccagaatgg cggtttacgt ttgccctttt tctttttgcg
    12841 atcttccgga atgcggtgaa tacggtcacg ctttttcgtc ccactccaga aacggcgcag
    12901 caggaaaatc agaccacccg caatgaccag gccgacgata ggggaaacaa tcagcgagga
    12961 gaaaattttg gttacttcac gcaggtttaa cgcatccatc accgatgagc cggttaacag
    13021 cgcgttggtt aaaccgatgc cgataatcgc accaatcaag gtgtgcgaac tggaggccgg
    13081 taaaccaaag aaccacgttc ccaggttcca gataatcgcc gccagcagca tggaaaagac
    13141 catcgccaaa ccgtgagttg accccatatt cagcaacaaa tcgattggta acatatggac
    13201 aatggcatag gcaacgctaa gtccgcccaa taacacgcca aaaaagttaa aaaatgccgc
    13261 catcaccaca gcaagttgtg gctgcatggc acgagtataa ataacggttg ccaccgcatt
    13321 cgccgtgtca tgaaaaccat tgattgcttc gtagaacaac acaaatgcca gagcaagtaa
    13381 taacaaaagc cccgtatata tatcaaggcc aacaaataaa tttagcatat attagattac
    13441 gccattttga atttacgaac ggacgcatta tcagtgactt taacggcatg ggcaaagtga
    13501 aatatcattt tttaacgata aaaagagtgt tatttcttgt gtatcagaca ataagcattc
    13561 attcaaaacc tttagaaata atcaaaacac aggtattcca ctggttaata tattcacata
    13621 tgaaatgaat aaatattgga atatataaat attgaatatt ttgattaatc cccgtactaa
    13681 ttattcttca taatcagtac ggggttgcta caacatgtat tactttttca ccacaattaa
    13741 cggttcaata tcactctctt ttttgatgac cagtgattca tcaccgcgca aacacgtccc
    13801 accgtagttg ccgccaacgg tgaaggtaca tacctgaatg tatttaccgt ccactttcgg
    13861 caaacaccac agttgctgat agatgttttt ctgctcggca aatttaccgc tggttttgtc
    13921 cagcacctct tcatgatggc tgaccaggtc gatattgcta ccacagcgac cggcgatcgg
    13981 tttcactgcg taacctgttt tcaccaattc atcattaaca gtgaaatcgg tatccagcag
    14041 gtaacgatgg tgcgggaaca gcgaccagag gatcggcaga atcgctttgt tgccggggat
    14101 caccgtccac agcggctcaa agaccagcac ttccgggcgc agcaatacgt cgataagacg
    14161 cacttcgttt tgcggatgac cggtacggat tggcaccgca gcaaactcac ggtcgctaac
    14221 ttcacgaatc tgatcaaacg cggtttccca cgcccaggtt ttccacacgc agttaaccag
    14281 tcgcccttcc ccatcaatca gttgcccggc agcatcccag cccagttcat ccagcccgcg
    14341 caagatacgc gtttcaaagc ccgcctggtg cagcgcctgc tccataaact gcgcgtgata
    14401 gttttcctcg atatctttgt cctgcatgat atggacaaac ggacgtgcac gactgtgttt
    14461 ccaggcacca gccagttcgt taatcagccc ttccgccgga ttgaagccgt tgcctttata
    14521 gccctgctcc gcccaacgtt cgaggatcaa gcccgcttcg gtatgacagg aggcggagtc
    14581 ggcgttgtat tcgtaaacct tcaggccacg ctcatccatg cagaaatcca tacgaccagt
    14641 gatcatatgg tgacggcgac gctgccagga gagacgcaaa cgtggccaga ggattttcgg
    14701 aatgtcgaac agcgccagca ggttgtcatc tttcagcacc ttgtcggttg cgtgaagata
    14761 catcagatgc agctcgttgg tggctttaat tagctcctgc tcggcactct cggtaatggt
    14821 gtagtaatga tacggatcct gattgatcac ctgaccgttg gcctgcacat aggcgttttg
    14881 tagcggatct ttttcatcca gccatttacc gtcaaactgg cctttgtttt ccagacgcgc
    14941 tccactgatt ttcagcagct cgcctgcaat ttccggctgc ggtaagctgt attcagtatc
    15001 ttccgtctgg atcatccagc ccagaatggt ggtgtcatca aaagtgtctt taagggtata
    15061 gcagccgttt tcgaccacca tctccagctc gcgcgtccac tgttgccctt gcggcaacgg
    15121 ggaatgaatc acgttctgtt ccgcaatacg gactttgttg ccatgcaatt gggtaatgat
    15181 ggcgacatgg ccagtgtctt taaattcacc gcctttatcc cagataagaa gcgcacccgc
    15241 gaccggcgca cgcgacgagc cgttaggaaa tgcctgcaat ggcaggatgt tgtcattaac
    15301 cacttctcgc aggaagcgca gcgagaaaat ctcccacgcc atacccacgt cagtaaagac
    15361 cacaccgtaa ttcagaaaga gaaaacggcg agcaaattca acgcattgcc acttgtggcc
    15421 catatattcg tcgtcgatat agctacggaa tacggcgtca tcttcgtatt cctgcggatc
    15481 gagagaactg taatctgaag agtagattgc taccccacct ggggcgtagc ccaataatgt
    15541 cccgaacggg gcatcctggc tggtcgttcc tttgctcatc actttacctt taacaataca
    15601 acctaagcag ttggcgtaat tgttgtgctc tgattacctg ctcagcgaca ttaaccggac
    15661 agaggtcaga ctaatcatac acctcatcgc agcggctggc gcaaagggtt aaaaaattca
    15721 cattctgtac agcaagtgac ctgctacact gcttcaacac taccactcag aaggcaactc
    15781 actatgacag acaatactta tcagcccgcg aaagtctgga cgtgggataa atccgctggc
    15841 ggcgcgttcg ccaatatcaa tcgcccgttt tctggtccga cgcatgaaaa aacgctgccc
    15901 gttggcaaac atccattgca actttattcg ctgggaacgc cgaacggtca gaaagtaacg
    15961 attatgcttg aggagctact ggcgctgggc gttactggtg cagagtacga cgcctggctg
    16021 attcgtattg gcgatggcga tcaattctcc agcggctttg tcgaagtgaa cccaaactcg
    16081 aagatcccgg cgctgcgcga tcatacgcat aatccgccga tccgcgtgtt tgaatctggt
    16141 tcgatcctgc tttatctggc ggagaaattt ggctacttcc tgccgcagga tctggcaaaa
    16201 cggactgaaa cgttgaactg gctgttctgg ttacagggtg ccgcgccgtt cctcggcggt
    16261 ggttttggtc acttttacca ttacgcgccg gtgaaaattg agtacgccat caaccgcttt
    16321 accatggaag ccaagcgcct gctcgacgtg ctggataagc aactggcgca gcataagttt
    16381 gttgcgggcg atgagtacac cattgcggat atggcgattt ggccgtggtt tggcaacgtg
    16441 gtgctcggcg gcgtgtatga tgccgcagag tttctggatg cgggaagtta taaacatgtg
    16501 caacgctggg cgaaagaagt gggcgaacgt ccggcagtga aacgtgggcg tattgttaac
    16561 cgcaccaacg ggccgctgaa cgagcagttg catgagcgcc atgacgccag tgattttgag
    16621 acaaatacag aagataagcg tcaggggtaa gggttggtgt tcgtcgcagc aagccatcca
    16681 ggccggataa ggcgttcacg ccgcatccgg caatcgtgca caatgcctga tgcgacgctg
    16741 ccgcgtctta tcaggcctac aaaagctaat catcacgcgc tggtaatgtc gtaatccatt
    16801 tggcgcagtg cgtctaatgt ggctttggct tcatcttcgt cgatgatgct catggcaaat
    16861 ccgacgtgta ccagcaccca ctggcccagc agatcggcag ggttaccttc gcaaatcagg
    16921 gcgatattca cgtcgcgctt gataccacat acttcaacct gcgcaagctg gtgaatatct
    16981 tcaccgacag ccagcacctg gcctggaacg ccaatacaca ttgttaactc cggttatttc
    17041 gattgccgga caaacgcgaa aaagaacctt tagcgggtgt tccctctcac cctaaccctc
    17101 tccccggagg ggcgagggga ccgtccgtgc gctttttagg cgttgtcctc agtcttattc
    17161 aacttcaata cttttgacga tcagcgaatc gccggtatcg acacgcaacc gctcgccgtg
    17221 acagagcgga cactgcgcat cgtgctgatg aatctccacc acctggctgc aatcccagca
    17281 ccaggcctgg gcgggtttat agacgatatg taaatcgcac ccttgcgcca ccgttccgtg
    17341 gcagacaatt tcaaaactaa aacggacggc gctctcctca acgcaggaga gcgcgccaat
    17401 ttccagccac acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc gttggataat
    17461 ttcaacggcg ctctggcaaa gcgacaactc atgcattttc gcccctcctg cggccaaaca
    17521 gcagggcgcg acgccctgcg tgtggtacat ccggattggt gactggcaac gaaaggatca
    17581 ttcgtgcgca gtcatccgtc aggcgttggc cttcttcaat cgacatgctg tgcgaaagcg
    17641 gcgacatcag cgaacaggag aggtattgcg aaacaccgtc cagttcgcca acggtaaagg
    17701 tcatggtgcc atacggcagt tgcagaccaa ttttttcact cactttgcgc agcggccaga
    17761 gttgatccgg gccggggaag ataactgcac tcagcatcca cggggtgatc acacaccccg
    17821 tccactgacc ttcgaacagc gtaaaatcag aaacatacac cggcattgaa ggatgcagaa
    17881 aagagagatc gtgcatcaaa cgccgggcaa tttcttcaaa cgctgcctgt acttgcgcct
    17941 tcggggaggt ctggaaacct gctatctctt cagtcatgaa tcgcctcccg tgggatggct
    18001 tccacgcctg attcgcgcag cgcagccaga acctgctcaa gcgcaggttc aatcattgct
    18061 tcaaccgtcg gcgttaagcc gatgtgtggc tccagcgatt ccgggatcac gccgaccagg
    18121 gtcagctttt tcggaaactc accggtgaag cgcagggccg acaatacgtc ggccaggcca
    18181 agctgatgcg gagagatttt gttggtaaac aacgccggaa cttcttcatc ccgcaggatc
    18241 atcatcgttc ccggagtgtt ctttttcgac acaatggcat ccgcaataat cagatgatcg
    18301 cgatttgcca tgtcgccaag cagctccatt cccgccgtgc cgccatcgag gatctcaaca
    18361 taatccggca gaatgtatcg ttgctctaac gcttcgacaa tccgcacgcc gatggcttca
    18421 tcggtcagca aaatattgcc gacccctaag actaaaatac gcattacaga accttcactg
    18481 aaaccacttc gttgccgtcg gcatccacta cgtgtaccgc acaggccatg cacgggtcga
    18541 aggagtgaat ggtacgcacc acttccagcg gtttattcgg atcggcaacc ggtgtaccca
    18601 ccagcgactg ctcgtaagga ccgacgtcat cattgaagtt acgcggacca gagttccagg
    18661 ttgatggaac aaccgcctgg tagttgctga tgataccgtc tttaataacc atccagtgag
    18721 agagcatacc gcgcggcgct tcgaggaagc caacaccttt gaactcaccc gttgccggaa
    18781 tgttcggctt cacaaaggtg gtgtgatcgc ctttgccgat attggtgatc agtgcactgt
    18841 attggttttg caggatatcc tgcaattcac agcagtgaac ggtacgacca ataatacggc
    18901 ccagcgtgga gtgcagctgt gccacttcca gcgtgttgcc agtcagtttc tgataaatcg
    18961 caacgatttc attcagtttg ttctgggtag attcgcgacc tgccgccagt ttcaccagca
    19021 tattagccag cggacccact tctaccgttt tgccgtagaa agtcggtgat ttcacccagg
    19081 aatatttccc gtcgtcagac caaccatcat aagccggaat ggtggtacct tcccacggcg
    19141 cctgtggcgc ttcgtcttta taccaggagt gcttcgcgct ttcctgaatg cctttgatca
    19201 ggtattcatc ggaatgagaa gtgatcggac gatacgagga cagatccgca ttctcaatgt
    19261 agcctcccgg gaacaggaag ctgccgtttt tgctgtcggt cgggaattcc ggcacgctca
    19321 ggtagttcac cgcaccttta ccgcgcgtca gccattccgg gtagaacgcg gcgataaccg
    19381 cggtatcaac cttataaacc tgctcaacaa agtcgctcag tttgtcgatg aaagacttga
    19441 tgtacatcag gcgctcaagg ttcaacacgc ccaacccgtc gaggttgatt gggttcgcga
    19501 caccacccac tgccaggttc tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc
    19561 ggttagcgtc acgctggcac tccagcgcct gcaggtagtg cgctaccgca atcaggttca
    19621 cttccggcgg cagtttcatc gccgggtgac cccagtagcc attggcgaaa atacccaact
    19681 gaccgctggc aaccagatct ttgatcttgt tctgaacttt agtgaactct tccggactgt
    19741 tcagatgcca ggtcgaaacg cctttcagca tttcggaggc tttggttggg tcagcttgca
    19801 gtgcagaagt gatgtccacc cagtccagcg ccgaaagctg atagaaatga acgatatggt
    19861 catgcgtggt gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta accggaacgt
    19921 cgatattcag cgcactttct gccgcacgaa cggaagacag cgcgtgagtg gtagtacata
    19981 cgccacagat acgttgcaca atcatccatg catcgcgcgg atcgcggttt ttcacgatct
    20041 cttccatgcc gcgccacatg gtaccggaag cccatgcttt cgaaacgacg ccattttcga
    20101 tttcgcaatc gatgcgtaaa tgcccctcaa tacgggttac cggatcaata gtaattctct
    20161 ggctcatgct ttgctcgcct catgacgatt atgatcgttt tgttttaaag gaggaagtat
    20221 cggcagtaga cgaatgagta cgatgtaagc gcaaatctca atagccacaa aaccaataga
    20281 aatcaacagt tcttcccagg tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac
    20341 caacgaatag gtcagacgcc aggttgcaca acctaacagt gcgctcagtg ctgacaggaa
    20401 cagcatgcgg gaatcattac gcagcttcgc cacacgcaga acgaccagcg ggaagagcat
    20461 cagcaggact tcaatccaga acatcacgga gtagaagtca ccggcaaacg ctaacgacag
    20521 cttgtcgcga tagataagct cgccaaagcg cagcccgatg aaaatcgcca gcaacacact
    20581 gatggtgttg gtcagcttga cgaacaggct cttttcatcc ggaccgttgc cacgcagacc
    20641 cgcctgcacc agtgaacctt caaagatgac aatcgagaag cccatgatga acgccgtcag
    20701 cagcgagaac agcggcaaca tttcatagct ctgccagagc ggatgcacct tgtagcccgc
    20761 cgagatcatc agcgacccca ttgaagactg gtgcatggtc ggcagcagcg caccgagcgc
    20821 gatgatgaag aacatcacct tgttcagacg ttgtagcgac accttccagc ccagacgttc
    20881 aaacagtgcc ggagcaaact ccagagccat cacgccgatg tagatggtca tacagaccgc
    20941 cgtctcgaac agtaccgagt tcacgttgaa gtgacccgga atgtagaagt acggcaggtt
    21001 ccagtagcga cccacgtcga tagtgatcga caagccaccc agtgagtaac caaacagact
    21061 cgccaacagc gccggacgca ccagcggatg gtattgccca cggttaaaga cgtataccgc
    21121 ccacgccagc gcccagccgc cacaggcaaa accggtgcca atcagcaggt caaacgcgat
    21181 ccacacgccc cacgggaagc cgccgttcag gtcagagaca gagcccagac cgaacaccag
    21241 acgcttcaca atcaggagca tacagatgac gattaacggt ccaaaaatca tgaccggttt
    21301 actgatgatt ttgccgccca gcggttgtgg atcatgactc atgatcgtct cctccgtcgt
    21361 gatggtcgtt tttggtgttg cgacgaacca gcacggttaa gcccgccagc acagccagtg
    21421 gtagcatcat gcctttatac agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa
    21481 gatcgtccag tttcggcagg tcgagatttt cataaggcac acccgtcagt accagaacct
    21541 gagtaccgcc gccctctttc tcgccgtaca gatgcggata atatttcggc accgtatgca
    21601 ggtaagtgtc gccagatttc agcgtctgac gcggatagtg gtattcgctg ccaggcttca
    21661 gcgccagacg ttttttcgcc tccgccatca gctcttcacg cgtaccgaaa atcaccgcgc
    21721 ccgccgggca cacttctacg cagccaggca gaccgccttt atcgagacgt tccacacctt
    21781 tctggttgca cagctcgcac ttatgcagcg caccaaacgg gttgttgtag tcgtacttcg
    21841 gcacgttgta cggacaggcg accatgcagt aacggcaacc ggtgcacacg tctttgtcgt
    21901 aatggacaat gccggttttc ggatcttttt tcagtgcaga gaccgggcac acagagacac
    21961 agttcggatc gacgcagtgc atacactgtt tcttaatgta cgcgtagccg ttctcctcct
    22021 ggtctttgtt gacccctgtg ccgctggtcc acacctgaat gatgttattg gtatacggcg
    22081 acagtttgtc gttgttcgac caggtctgtt ccccttgcgg gttacgttca gggaagttga
    22141 tatcctgaca cttggtgacg caagcctggc agcctacgca caaggtcgag tcgtacaaca
    22201 tccccagcga tcccggaatt ggcgggcggt tttcagcagc cgcatgactg acagacggca
    22261 gcgcgcccgt cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg
    22321 ttattctccc cgtgagtcag cgttatcttt cttttgctga cgacccagtt cacgcaccgc
    22381 catcacgctg acaccggcaa ccagcccaac cacaccgccg agcaaaccaa tagcgcctgc
    22441 agagacgttg ccgccctctt tagcgttaac atccggtttc tgtgaacgcg gagtctgatt
    22501 ttcgacgttg gcaagctgat ggatgccttt atggaagccg ataccttttt cgttacagcc
    22561 atagcacggg tgaccaatcg ccaccggcca cacaccgcca acatcgcaga attgcagcgt
    22621 tgagcagttg ccgtaagttt ctggcccttt acagccgagg tggtacaggc accagccttc
    22681 gcggtggcct tcatcaccga actctttggc aaaacgacca gcatcgaagt gcgggcgacg
    22741 ttcgcagtgt tcgtgaatca gacggccata ggcgaaggtc ggacggtttt tgtcatccag
    22801 tttcggcggt ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt
    22861 cggcgggcag cccggaatat tgataacggt tttgcctggc agaacttctt gcaagctgac
    22921 tgcgccagtt gggttaactc cagctgcggc aacaccgccc cacgcggagc aggaaccaat
    22981 agcgataatg gctgctgagc cttccgccgc tttgcggatg tgatccacaa tcggctcacc
    23041 ggcaaccatg caataaatac cgttatcttt taatgggatg gaaccatcca ccactaacac
    23101 atactgccct ttgtacttct cgagagcgtt atgtttgttc tcttcgacct gatgaccgaa
    23161 ggcggcggaa agcacttcgt gatattccag agagatagtc tccagcacga ggttttctac
    23221 cgttggatgc gttgcacgaa gcagagattc cgtacaaccg gtgcactcct gcgcgccaat
    23281 ccagataact ggcggacgct gcgggttagt aaccgattcg gccatctctg cagcggcttt
    23341 gctacttaac cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt
    23401 aatgccgtga gaatggatga gggtgttatc tccagtcatt tatagttatt ccgttgcgag
    23461 gacctggcat atattttgcc tcaatcgcaa aatcaataat gcgatcgatg cgccatttac
    23521 cacacattta ttatggttat cgatatcatg atactgcggc ggggcgctaa aacgaaggga
    23581 agaagcatta gtgtaattaa ttaaagcaag ataatacgta tgtttgatca attttcgagg
    23641 caataaccgg ccataaaaaa gcgcaacgta gaacaggaat tattctcacg aaagaaatcc
    23701 attaccatct ctttatccag tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta
    23761 agctggcgat ctccggtctt ttcgtacatc cggtgtgagt agcctaatac aaaaccgcgt
    23821 ttataatcgg cacagaattt tgtcacatta accgcactgt cggcatgcgt tgcttttaat
    23881 cccgccatta agcctttacc aaaatggtta ttcatcgttc acctcattcg ttatatatcg
    23941 ccatatatag cgatatttta ggcaaagaac gacttttgcg cacgctattt tttcatactt
    24001 gtttaagacg taatcccgta agagataaac ctatcaggag agatatttat cgaaccattt
    24061 cagtgtcctt tgccaggcaa gatcggcggc agatttgtca taacggggcg tggaatcatt
    24121 atggaatccg tgattaaccc ccggatagat ataagcctca taaaccttat tattgtcttt
    24181 caacgccgcc tcgtaaggag gccagccctc gttgattcgg gtgtccagtt ccgcgaagtg
    24241 gagtagtaaa ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac gaccataaaa
    24301 cggcaccgcg caggccagtt ccggatacgc gacagccgcc gcgttcgata cgccaccgcc
    24361 atagcaaaat ccggtaatac ccactttacc tgtcgcttgc ggatagcgtt gcataaactc
    24421 aattgcggca aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg
    24481 acctttatca tcatttcccg gataacctcc aacggaactt aatccgtcag gtgccagggc
    24541 gatatacccc gccttcgcca ctcgccgtgc cacatcttcg atatacggat tcagtccacg
    24601 attctcatgc accaccccca cggctggcgt tttgccgctc atttttgcgg gtttcaccag
    24661 ataaccccgt acctcgccgt gaccatttgg cgaaggatac gtgatgtact cagcaacaat
    24721 ctccgggtcg gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa
    24781 caacgctaat gccgtcatcc cgcccaccgc atacttcgcc gcaaggttga ggaactcacg
    24841 tttcgaaatt ttcccgtgaa cgtaatagtc gtagtaatcc aacaactctt gtgggaaatc
    24901 tttggcggtt aaacgcggca tcgttgcatt cctcaactgg tgttttttta agcaaagcat
    24961 aagcgcgtgt ttttgcccag tttttcgtca ctctgtgagc cagactacgg gatacgcgct
    25021 ggcgaatcgc taaactagaa acattgtttc gaaattgaac ggaggaaagg agaggtcatg
    25081 gtctggttag cgaatcccga acgttacggg cagatgcagt accgctattg cggaaaaagt
    25141 ggtttacgcc tgcccgcatt atcgctcggt ttatggcaca atttcggtca cgttaacgcg
    25201 ctggaatcac agcgtgcaat cctgcgtaaa gcgtttgatt taggcattac gcactttgat
    25261 ttagccaaca attacgggcc gcctccagga agcgcagaag agaactttgg tcgcctgctg
    25321 cgcgaggatt ttgctgctta tcgcgatgaa ctgattatct ctaccaaggc tggctacgat
    25381 atgtggcccg gcccgtacgg ctctggcggt tcacgtaaat acctgctcgc cagcctcgac
    25441 caaagcctga agcgtatggg gcttgagtat gtcgatatct tttactctca tcgcgtcgat
    25501 gaaaatacgc cgatggaaga aaccgcctct gcgctggctc atgcggtaca aagcggtaag
    25561 gcgctgtatg tcgggatctc ctcttactcg ccagagcgga cgcaaaaaat ggtcgagttg
    25621 ctgcacgagt ggaaaattcc gcttttaatt catcaacctt cgtacaattt actgaaccgc
    25681 tgggtggata aaagcggcct gctggatacc ctgcaaaata acggcgtggg ctgtattgcc
    25741 tttactcctc tggctcaggg attgctgacc ggaaaatatc tcaacggcat tccgcaagat
    25801 tcacggatgc atcgtgaagg gaataaagtt cgtggtctga cgccgaaaat gcttaccgaa
    25861 gccaacctca acagcctgcg cttattgaat gaaatggcac agcagcgtgg acaatcaatg
    25921 gcgcaaatgg cgttaagctg gttgctgaaa gatgatcgcg tgacgtcggt attgattggt
    25981 gccagccgcg cggagcaact agaggagaac gtgcaggcgc tgaataatct gacatttagc
    26041 accgaggagc tggcgcagat tgatcagcat atcgccgatg gcgagctgaa tctgtggcag
    26101 gcgtcgtccg ataaatgacc tgttaataac gggcgcgcga accgcgcccg aatcagatca
    26161 gtgattatga cgagtcagtc ggtcaagata gcccatcaca aatgcagaaa gcacaaacgt
    26221 cagatggata atgacgtacc acatcagttt gttatccggg acatttttcg catccataaa
    26281 gacgcgcagt aagtgaatgg aagaaattgc cacaatcgac gctgctactt tgtttttcag
    26341 cgacgttgcg tccattttcc ccagccactt cagcttctct ttgttctcgg agatatccag
    26401 ctgtgagacg aaattctcat aaccggaaaa catcaccatc accagcaaac cgccaaccag
    26461 cgtcatatcc accagcgaca gcaacacgag gatcaaatct gattccgcca tcgagaagat
    26521 attcggcagt acgtgaataa tctcctggaa gaacttcagc gccagggcaa ctaacgccag
    26581 cgaaaggcca aagtacacgg gggcaagcag ccagcgagaa gcatacattg cattttcaag
    26641 aaaacgttcc atacattcct gtcgtgtagt taaaacgggc aacagtatat cgcaattgag
    26701 taaacagcac gcaacagcaa atcaacatat acgttaaacc tttgtcgcat aattctgagg
    26761 tttagggatc ttcctggttc ccttatcaac gcatcgagag cgtctaccct taaaggacgt
    26821 tgaacctgaa gggagaaaaa cgatgtctca tttaaaagac ccgaccacgc agtattacac
    26881 tggtgaatat cccaaacaga aacaaccgac gccaggcatc caggcgaaga tgacaccgat
    26941 accggattgc ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc gtaaagcact
    27001 ggtgacaggg ggcgattccg gaataggtcg cgctgccgcc atcgcttacg cgcgtgaagg
    27061 ggctgacgtg gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg atgtgaaaaa
    27121 gatcattgaa gaatgcggac gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa
    27181 attcgcccgt tcgctggttc acgaagcgca caaggcgtta ggcgggctgg atattatggc
    27241 gctggtcgcc gggaaacagg ttgccattcc ggatattgca gacctcacca gcgaacagtt
    27301 tcaaaagacc tttgccatta acgttttcgc gctgttctgg ctaacccagg aagcgatccc
    27361 cctgctaccg aaaggtgcaa gtgtcatcac cacttcgtca atccaggcat accagccaag
    27421 tccgcattta ctggactatg cggctacgaa ggcggcgatt ctgaactaca gccgtggcct
    27481 ggcaaaacag gtcgcggaga aaggtattcg ggtgaatatt gtcgcgccag gccctatctg
    27541 gacggcactg caaatttccg gcggacaaac gcaggataag atcccgcagt ttggtcagaa
    27601 aacgccgatg aagcgtgcgg ggcaaccggc ggaactggcc cctgtatatg tttatctggc
    27661 aagtcaggag tcgagctacg tcaccgcaga agtgcacggc gtgtgcggcg gcgagcattt
    27721 aggttaaaaa aatgcccggt tgtgaaaagc aaccgggcat cattgtgaat tcactcttac
    27781 ctgatgtggc gaaaacgcct tatccgccta caaacgcatg aagattcaac atgttgcaat
    27841 agattttgta ggcctgataa gcgtagcgca tcaggcattg agcacctaat gccggatgcg
    27901 tcgtgaacgc cttatccggc ctacaaacgc acgaagattc aacatgttgc aatagatttt
    27961 gtaggcctga tgagcgtagc gcatcaggca attctacttt acttcgcttt ggcggtttct
    28021 tcgccgacca gacctatctt caggtaaccc gcctgatgca gcgtatccat taccttcatc
    28081 aacgtctcgt aatcgacggt tttgtccgct cggaagaaga tggtggtgtc tttcttgcct
    28141 tcggttaacg cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc gttaccgata
    28201 aacatcgagt tgtctgcctt caccgacaga taaaccggtt tttccgggcg cggctgcggc
    28261 gtgctggtag aagcaggcaa gttcaccttc acatctaccg tcgctaacgg tgccgccacc
    28321 ataaagataa tcagcagaac caacatcacg tcgataaacg gcgtcacgtt gatatcatgc
    28381 atttcgccgt tatcgtcgag gttttcgtta agatgcattg ccatcggaca ttatcctgcg
    28441 cgtaattttt gtgcgacacg aaccggatgc gcagcggcgc tggcttccag atccaggtca
    28501 cggctttgca gcaacaatac ctgcgctgca acatcaccca gcatcgcttt aaagccgcca
    28561 atctggcgtg caaatacgtt atagataacg accgcaggaa tcgctgccac gaggccgatt
    28621 gccgttgcta acagagcttc tgcgataccc ggcgcaacga ctgccaggtt agtggtctgc
    28681 gtttgcgcga taccaataaa gctgttcatg atgccccaga ccgtaccaaa cagaccaacg
    28741 aacggagaaa tcgcgccgat ggttgccaga tagccgttac cgcggcccat ttgacgaccc
    28801 actgcggcga cccgacgctc cagacggaag ctggtacgtt ctttaatacc ttcgttatcg
    28861 tcgctgcctt ctgacagttc cagctcattc tgcgcttcat tgagcaaatg caggcttaag
    28921 cttttgctac caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg tgcttcagcc
    28981 aacagttgct gctcgcgctt aaggcgacgc ttctgattga agaactctac gctcttactg
    29041 aagaagattg cccaggtgac tacagaggcc aaaataagcc caatcatcac gcacttaacg
    29101 acaatatcgg cgtactgata cataccccag acggaaaggt ccgtctgcat taaattatta
    29161 cccactgtgt atctccagga cgcaagtcac aaaatctgcg cataataata tcaaaacgac
    29221 gtcgaattga tagtcgttct cattactatt tgcatactgc cgtacctttg ctttcttttc
    29281 cttgcgttta cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt tctgctttat
    29341 gccaattctt caggatgcgc ccgcgaatat tcatgctagt ttagacatcc agacgtataa
    29401 aaacaggaat cccgacatgg cggacaaaaa gcttgatact caactggtga atgcaggacg
    29461 cagcaaaaaa tacactcttg gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt
    29521 atttgagagt atggaagcca aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt
    29581 ctatggacgg cgcggaacgt taacccattt ttccttacaa caagcgatgt gtgaactgga
    29641 aggtggcgca ggctgcgcgc tatttccctg cggggcggca gcggttgcta attcgattct
    29701 tgcttttgtc gaacagggcg atcatgtgtt gatgaccaac accgcctatg aaccgagtca
    29761 ggatttctgt agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt ttgatccgct
    29821 gattggtgcc gatatcgtta agcatctgca gccaaacact aaaatcgtgt ttctggaatc
    29881 gccgggttcc atcactatgg aagtccacga cgttccggcg attgttgccg ccgtgcgcag
    29941 tgtggtgccg gatgccatca ttatgatcga caacacctgg gcagccggtg tgctgtttaa
    30001 ggcgctggat tttggcatcg atgtttctat tcaggccgcc accaaatatc tggttgggca
    30061 ttcagatgcg atgattggca ctgccgtgtg caatgcccgt tgctgggagc agctacggga
    30121 aaatgcctat ctgatgggcc agatggtcga tgccgatacc gcctatataa ccagccgtgg
    30181 cctgcgcaca ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga aagtggccga
    30241 atggctggca gaacatccgc aagttgcgcg agttaaccac cctgccctgc ctggcagtaa
    30301 aggacacgaa ttctggaaac gagactttac aggcagcagc gggctatttt cctttgtgct
    30361 taagaaaaaa ctcaatgatg aagagctggc gaactatctg gataacttca gtttattcag
    30421 catggcctac tcgtggggcg ggtatgaatc gttgatcctg gcaaatcaac cagaacatat
    30481 cgccgccatt cgcccacaag gcgagatcga ttttagcggg accttgattc gcctgcatat
    30541 tggtctggaa gatgtcgacg atctgattgc cgatctggac gccggttttg cacggattgt
    30601 ataacattgc cacttttgga caattttgca gacattttat tgtgaaaagt cttaaattgt
    30661 tgcgtccggg atcaaggcgt cccggacgat tcaggagtac aataggcaga taaaggctta
    30721 aacgctgttc cacaggaaag tccatggctg ttattcaaga tatcatcgct gcgctctggc
    30781 aacacgactt tgccgcgctg gcggatcctc atattgttag cgttgtttac tttgtcatgt
    30841 ttgccacgct gtttttagaa aacggcctgc tgcccgcctc atttttgcca ggcgacagct
    30901 tgttgatact ggcaggcgca ttgattgccc aaggggttat ggattttctg cctacgattg
    30961 cgattctgac cgccgcagca agtctgggct gctggctaag ttatattcag gggagctggt
    31021 tagggaatac caaaacggtg aaaggctggc tggcacagct tcctgctaaa tatcaccagc
    31081 gcgccacctg catgtttgac cgccacggtc tgctggcgct gctggctgga cgttttcttg
    31141 catttgtccg tacgctgctg ccaaccatgg cgggaatttc cggtctgcca aaccgccgct
    31201 tccagttttt caactggtta agtggattgc tgtgggtcag cgtggtaacc agttttggct
    31261 atgccttaag tatgattccg ttcgttaaac gccatgaaga tcaggtaatg acgttcctga
    31321 tgatcctgcc aattgccttg ttaaccgctg gcttgttagg cacgctgttt gtggtgatta
    31381 aaaaaaaata ctgtaacgcc tgacgatttt ccccgttccc ggctgctgta ccgggaacgt
    31441 atttaattcc cctgcatcgc ccgcattctt gccgcatctt cccccggcgt cacaccgaag
    31501 taacgtttaa actcacggct aaattgcgat gcgctttcat agccgacgcg catcgctgct
    31561 gcgctggcct tcatgccgtc atggatgatc atcatccgcg ccttatgcag acggtaattc
    31621 ttcaaatact gcaacggcga ggtgctggtg acagacttaa aattatggtg gaacgccgat
    31681 acgctcatgt tggcttctgc cgccagttgc tcgacgctca ggttttcggt gtatttattc
    31741 tcaattcgtt tcagcacgcg gctaatcaga ctgaagtgag tctggcgact gaccagcgcc
    31801 agtaacgcgc cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg gatgatctgt
    31861 ttgccgagaa tacgcgcatc cagtggtcgc tccatcacgt cgagtaaccg ctccgccgcg
    31921 cataaaatct cttctgataa cgtggcggag ttaatcccgc tggctgccat cgacggctgg
    31981 aaatgctcat cttcgccaat gtccatcaac agttcctgta actgcaaaat atcgacattg
    32041 agacgcaacc ctgccagcgg cacctctgac gtggcatagg tttcgcactc aaacggcaac
    32101 ggcaccgtca gcagcaggta ttcattggca tcataacgaa acacgcgttc attgatataa
    32161 ccgattttat gcccggaaaa gagaattatg atgccaggct cgtacatcac cggtgtacgt
    32221 gcgaaaggcg tctcgccata caacaaacgc acatcgggca acagttctga caaactattt
    32281 tctttatttt tcagtttatt aactttatcc gccagcaagc gacaaatctc ttcacgtttc
    32341 atatcgcgta atttcttagg aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt
    32401 tctccagcac tctggagaaa taggcaagac attggcagaa atgagcattg agagccaggg
    32461 cgctggcgat cacaatgaaa aacatcaggc agatcgttct ctgccctcat attggcccag
    32521 caaagggagc aagtaatgaa caactttaat ctgcacaccc caacccgcat tctgtttggt
    32581 aaaggcgcaa tcgctggttt acgcgaacaa attcctcacg atgctcgcgt attgattacc
    32641 tacggtggcg gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga tgccctgaaa
    32701 ggcatggacg tgctggaatt tggcggtatt gagccaaacc cggcttatga aacgctgatg
    32761 aacgccgtga aactggttcg cgaacagaaa gtgactttcc tgctggcggt tggcggcggt
    32821 tctgtactgg acggcaccaa atttatcgcc gcagcggcta actatccgga aaatatcgat
    32881 ccgtggcaca ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc gatgggctgt
    32941 gtgctgacgc tgccagcaac cggttcagaa tccaacgcag gcgcggtgat ctcccgtaaa
    33001 accacaggcg acaagcaggc gttccattct gcacatgttc agccggtatt tgccgtgctc
    33061 gatccggttt atacctacac cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc
    33121 tttgtacaca ccgtggaaca gtatgttacc aaaccggttg atgccaaaat tcaggaccgt
    33181 ttcgcagaag gcattttgct gacgctaatc gaagatggtc cgaaagccct gaaagagcca
    33241 gaaaactacg atgtgcgcgc caacgtcatg tgggcggcga ctcaggcgct gaacggtttg
    33301 attggcactg gcgtaccgca ggactgggca acgcatatgc tgggccacga actgactgcg
    33361 atgcacggtc tggatcacgc gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa
    33421 aaacgcgaga ccaagcgcgc taagctgctg caatatgctg aacgcgtctg gaacatcact
    33481 gaaggttccg acgatgagcg tattgacgcc gcgattgccg caacccgcaa tttctttgag
    33541 caattaggcg tgccgaccca cctctccgac tacggtctgg acggcagctc catcccggct
    33601 ttgctgaaaa aactggaaga gcacggcatg acccaactgg gcgaaaatca tgacattacg
    33661 ttggatgtca gccgccgtat atacgaagcc gcccgctaag ctttttacgc ctcaaacttt
    33721 cgttttcggg catttcgtcc agacttaagt tcacaacacc tcaccggagc ctgctccggt
    33781 gagttcatat aaaggaggaa cgtatggcta atccaaccgt tattaagcta caggatggca
    33841 atgtcatgcc ccagctggga ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg
    33901 ccattcaaaa agcgttagaa gtgggttatc gctcgattga taccgccgcg gcctacaaga
    33961 acgaagaagg tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa gaactgttca
    34021 tcaccactaa gctgtggaac gacgaccaca agcgcccccg cgaagccctg ctcgacagcc
    34081 tgaaaaaact ccagcttgat tatatcgacc tctacttaat gcactggccc gttcccgcta
    34141 tcgaccatta tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag ggattaatca
    34201 aaagcatcgg cgtgtgcaac ttccagatcc atcacctgca acgcctgatt gatgaaactg
    34261 gcgtgacgcc tgtgataaac cagatcgaac ttcatccgct gatgcaacaa cgccagctac
    34321 acgcctggaa cgcgacacac aaaatccaga ccgaatcctg gagcccatta gcgcaaggag
    34381 ggaaaggcgt tttcgatcag aaagtcattc gcgatctggc agataaatac ggcaaaaccc
    34441 cggcgcagat tgttatccgc tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg
    34501 tcacaccttc acgtattgcc gaaaactttg atgtctggga tttccgtctc gacaaagacg
    34561 aactcggcaa aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt
    34621 tcggcggcta acgtgcaaat tctcccggtg gcggtaatgt tccgctaccg gacttttcag
    34681 aaatcattta ttcccctcgc gtcccgcccg ttgttactct tccttgttca ggaatgccaa
    34741 atataaggac atcatcatgc agagccggaa gctcttaaaa gaacaactca tctatatccg
    34801 ggataaacgc aacggagagg tgaaaaacag atgaaaataa tacttctgtt tttagcagcc
    34861 ctggcaagtt ttaccgtaca cgcgcagcct ccttcacaga ccgtagaaca aacagtccgg
    34921 cagatttatc agaactataa atcagatgcc agtacgcctt atttcggtga aaccggagag
    34981 cgggcgataa cttcagcgcg tattcaacag gcgcttaccc tgaacgacaa tcttacacta
    35041 ccgggcaata ttggctggct ggattatgat ccggtttgtg attgtcagga ttttggcgat
    35101 ctggtgttag aaagcgtagc gataacccaa actgacgtcg atcatgccga tgctgttgtg
    35161 cgctttcgta tctttaaaga tgataaagaa aagaccacac agacactgaa aatggtggcg
    35221 gaaaatggtc gttgggtcat tgatgatatt gtcagcaatc atggcagcgt cttacaagca
    35281 gttaatagcg agaatgaaaa aacgctggcc gctttagctt cgttgcagaa agaacagcca
    35341 gaatcctttg ttgctgaact ctttgaacat attgctgatt atagctggcc gtggacgtgg
    35401 gtggtttccg actcttaccg ccaggcggtt aatgccttct ataaaaccac cttcaagacg
    35461 gccaataatc ccgatgaaga tatgcaaata gaacggcaat ttatttacga caatccgatc
    35521 tgttttggcg aagagtcgct attttcacgc gttgatgaaa ttcgagtcct ggagaaaacc
    35581 gccgattccg cccgcattca tgttcgtttt acgctgacca atggcaacaa tgaagagcaa
    35641 gagctggttt tacagcggcg cgaaggtaag tgggaaatcg ctgattttat ccgcccgaac
    35701 agcggcagcc tacttaagca gattgaggca aaaaccgccg ccagattaaa gcaatgagct
    35761 gaattaaata acaattagcc ggaacaataa ataaaaggga acactatatg aaaacgattt
    35821 tcaccgtggg agctgttgtt ctggcaacct gcttgctcag tggctgcgtc aatgagcaaa
    35881 aggtcaatca actggcgagc aatgtacaaa cattaaatgc caaaatcgcc cggcttgagc
    35941 aggatatgaa agcactacgc ccacaaatct atgctgccaa atccgaagct aacagagcca
    36001 atacgcgtct tgatgcacag gactattttg attgcctgcg ctgcttgcgt atgtacgcag
    36061 aatgataaaa aaatccccgg cagcatgtca gttgccgggg atttttttta acgtccaacc
    36121 gccgctttag ggcgtttctt cgcaccagca ttcaccggac gagattgcgt agacgacgct
    36181 ttttttgccg tagcaggcgt ctgacgctga gtcgccatcg gcgtatgttt agtcaacgcc
    36241 ggacgggtat tgcggttctg gcgacgagct tcacgcatct cttcaatggt tggcgcaggc
    36301 actaagcaat cgcgacggct gccaatcaga tgctttttgc ccatcgcttc cagcgcctgg
    36361 cggattaacg gccagtttgc cggatcgtgg taacgcaaca acgctttatg caaacgacgc
    36421 tgtttgtcgc ccttcggtac gaagacgcct tcgctcttat aaccaatctt cgccagcggt
    36481 tttttcccgg tgtaatacat ggtggttgag ttcgccagcg gcgatgggta gaagttctgt
    36541 acctggtcga gacggaaacg atgctttttc agccacagcg ccaaattcac catatcttca
    36601 tcacgcgtac cggggtgcgc agagatgaaa tacgggatca gatactgctc tttacctgcc
    36661 tgtttcgagt aggtatcgaa cagctcttta aagcggtcat agctgcccat gcccggcttc
    36721 atcatcttcg acaacggccc ttcttcggta tgttccggag caatcttcag ataaccgccg
    36781 acgtgatggg tcgccaactc tttgatatag cgcggatctt ccacggcgat gtcataacgt
    36841 acgcctgagg cgataaggat ttttttaatg cctttcagat cacgcgcacg gcgatagagg
    36901 ttgatcgtcg gttcgtggtt agtgtccatg tgcggacaaa tatccggata aacgcacgac
    36961 aaacggcgac aagtttgttc agcgcgtggc gatttgcagc gcaacatata catgttggca
    37021 gttggcccac cgagatcgga aatcacgccc gtaaaacctg gaacggtgtc gcggatcgct
    37081 tcgatctcat taatgatcga atcttccgaa cggctctgaa taatgcgccc ttcgtgctcg
    37141 gtaatagaac agaaagaaca gccgccaaag cagccgcgca taatgttgac cgaaaaacgg
    37201 atcatttcgt aagccggaat acgggcattg ccataggccg gatgtggcac gcgcttgtac
    37261 ggcagcgcaa aaacgctgtc catctcttcg gtagaaagcg ggatagcagg cgggttgatc
    37321 cacacatagc ggtcaccgtg tttttgcatc aatgcgcggg cgcagcctgg gttggtttcg
    37381 tggtgcagaa tgcgcgaagc atgggcgtac agcactttat cgcccttcac tttctcgaaa
    37441 gaaggcagca atacgtaggt tttttcccac ggtttctggc gcggtggctg cacgattaca
    37501 gctttcgctt cctgcttttt cggcgcaaca ggtttgttat ccgcacacgg caaatcttcg
    37561 ccatacggat gcgggattgg gtcgattttc cctggggtat caagacgggt ggaatccaca
    37621 ccgctccagc caggcagcgc ctcttttacg ataattgcgg tattacgcac atcgcggatt
    37681 tcactaatcg gctcgcccat cgccagacga tgcgctactt ccaccagcgg acgttcaccg
    37741 ttaccaaaca tcagcatgtc ggctttcgaa tctaccagaa cggaacggcg cacggtatcg
    37801 gaccagtaat cataatgcgc ggtacggcgc aggctagcct caataccgcc gaggatctcc
    37861 ggcacatctt tccacgcctc tttacaacgc tgggtataaa ccagcgtggc gcgatccggt
    37921 cgcttacccg cgacattatc tggcgtgtag gcatcatcat gacgtaaacg gcgatcggcg
    37981 gtatagcggt tgatcatcga atccatgttg ccagcagtaa caccaaaaaa cagattcggt
    38041 ttacccagac gcataaagtc gtctttgctg ctccagtccg gctgggcgat gatcccgacg
    38101 cgaaagccct gcgcttccag catacgaccg caaatcgcca tcccgaagct tgggtgatcg
    38161 acatacgcgt cgccagtaac caaaatgatg tcgcagctat cccagccaag ttgatccatc
    38221 tcttcacgag acatcggcaa aaacggtgcc ggtccaaaac aggcggccca gtactgcggc
    38281 caggagaaca ggtcgcgatc cggttggatc agggagatag agctcatttt gcttccagaa
    38341 atgataaaaa aataatcaaa ggccggggat tataagccgg aacgaaagag aaatcgaaag
    38401 gtattccata ctcgccctcc tcgggcgagt atgaagatta cggtaccgga ttgaccaaca
    38461 gttgcccaat cgagccacgg tccgccattt ccagcgtctg actgttgaag tagaacggga
    38521 agtgcgccca ggaaggctga ccgaaataaa caagcagctc cacttgtccg tcaacccaaa
    38581 cggtatcttt ccagcctctg tcttccggga acggcatcgc gccattcacg ttacggatct
    38641 ggaacattac gccttcaata tggaacgctt gcggctcgtc cgcgcgtacc gtccagcgtt
    38701 cccacgttcc ttgctgcgcg gtgacatcaa tacggttgac gtcccacagc tgtccattaa
    38761 tacccgggtc atcacccaga ctgatatcgc gactgcgaat tggcgaacca gccatgattt
    38821 cagttggcag caagcgcatc ggaagactgt ctgtgaccag cggcagaagg ccggttgggc
    38881 gcagcgttag caccaaggta gaaaccagaa tactggatgg ctcaaagaag ccgcgaatac
    38941 gatcaacaat gctcgccgct tcaccacagg tgatcgacac ttcatcgccg ttgctcatat
    39001 ccaccagaat ctcgcggcgc tcgcccggtg ccagcgaaag ttgcttcacc gatacaggag
    39061 caggcaggaa tccctgatcg ccagaaatca catgtaacgg gcgaccatcg ctcatctgta
    39121 gttgatagcg acgagagttc gacgcgttca gcagtcgcaa gcgcacccag ccacgcgaga
    39181 cttcaacgta cgggctttgt acaccgttaa ccagcagcgt atcaccaaca aagccgccgc
    39241 ttcccggttc gttgtattct ggcgtaccaa agttatccag ccgtttatcc tggataatga
    39301 ccggaaaatc atccacacca taatggttgg ggataggcag cgacttgctg acttcatctt
    39361 ccaccagcca cattccggca aggccgttat agacctgctg agccgtgcgg ttgggagtat
    39421 tggcgtgata ccacagagta gctgcgttct ggcgaatagg cagtacgggt gcccagtcag
    39481 cgtttggcga catcatccgt gccggaccgc ccatcagcgg gcctggtacc tgtagcccgg
    39541 ccaccgtcat tgagacattt tctgtcaggc ggttgctgta aataagctta acatcgtcgt
    39601 ccttccagac gcggatagtc ggccccaggt aacgaccatt gattccccag accgacgcgc
    39661 gtgtccctgg cgtaaatgac cagtgcgcac gttgtacagt cataaacagc ggttgcccac
    39721 ggcgagattc aagtagcggc ggaacgggta gcggttgctg ttgcccggct gcgctggcct
    39781 tcaggggaac agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac tgacgccgac
    39841 tgagtgacat atttgctcca tgtaaaactg gctaaagtaa taccgtgcag gaaagtgttc
    39901 cccgcaaaaa tacagattgc cgacttaaac ttttccggcg gcttcgcgtt ctgcgacttc
    39961 tttatcgagc tcggcgattt tttgttccat tatcgaacga caatgggcag ccagctcacg
    40021 aacctgatct ttgccatact gactgacgtc aattggcggc agcatttcga caatcaccag
    40081 accgttgtgc agtcgattaa gattaatctt attcgaagtt gtagagacgc acacgggaat
    40141 aatcgggacg cccgccgcaa ttgccgcgtg aaatgctcca gtcttgaacg gtagcaggcc
    40201 gcgaccacgg ctgcgggttc cttccgggaa catccagatg gaaatacggc gttttttgaa
    40261 atgattcacg acttccgcaa tggtgccgtg agctttagtg cgattgtttc tgtcgatcaa
    40321 taagttgccg gttaaccagt acaactgccc gaagaagggg atccacagca agctcttttt
    40381 acctaccgtc accgtcggcg gttgcacaat gttcgatgct gtcaccatgt catagttgtt
    40441 ctggtggtta gcgatataga tagcattgcc gtagctttca gcatctgcag gtttgcggca
    40501 ctcaactttc aggccgaaca gtggcgcaag acggccaaac atatgcccaa aggtggccac
    40561 atgtttcggg ttacgcgggc tgaaaaggca gtaaatggag ccgaatacac agactaagat
    40621 gctgtaaatc acggtaataa taagacgaaa gatatatagc ataacaccct ctgaaggtct
    40681 gagagactgg cattgtacgt cacctgaaat caggttaggg cattgttaaa agccgctctc
    40741 tggaaaagcg tattgttaat cgcaacgcat gaataaacaa cggtttcaag ggaattttta
    40801 tcgtttccgg gggttgaata cgctgccgga tgacgactta acgtttcatc cggcgttcct
    40861 tgctagcggg aggaaacagc gccctccccg gcatattact cttcgctatc accgctgctg
    40921 gcacggcgag gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg catcaacgta
    40981 ccgcggcgtc cacgttcgcc agtgactttc tgtagctctt ccggacgcag tttaattttg
    41041 cgtttcccaa catgaatggt cagcgtgctt tgcggcggca gaacgtacag ttgcgccaga
    41101 ccatcctcgc cacgcgcggc ttctgccgat ggaatgttga taatcttgtt gcctttgccc
    41161 ttcgacagct gcggcagatc gcttaccggg aacatcaaca tacggcctgc ctgagtgatt
    41221 gccagcagca tatcggaagc atcttcaatc accaccggcg gcataacatg ggcattttcc
    41281 ggtaaggtga tcaaagcctt acccgcacgg ttacgcgcta ccagatcgtt aaaggtgcag
    41341 acgaaaccgt aacccgcatc ggaagccatc agcagtttct gatcgtcgct ttccatcagc
    41401 atatggtcaa cggtcgcccc aggcggcaac gttaatttgc cggtgagtgg ttcgccctga
    41461 ccacgcgccg acggcagcgt aatcgggtcg atggcatagc tacgaccggt ggaatcaaca
    41521 aacactaccg gttggttact cttacctttc accgccgctt tgaagctatc acccgcttta
    41581 taattcaggc ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca gcccatctgc
    41641 gacagcacaa tggtgacagg ttcagacggc agcatgtcgt gctcgctcat cgctttcgct
    41701 tcttcgcgtt cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc gtctgcctgc
    41761 agttctttct tcagcaggtt attcatttta cgctcggaag ccaaaatgcc ctgcaactgg
    41821 tcgcgctctt tttccagctc actctgctca ccgcgaatct tcatctcttc cagtttggca
    41881 agatgacgca gtttcagttc gaggatcgct tccgcctggg tttccgtaag gccaaaccgc
    41941 gacatcagcg ccggtttcgg ttcatcttcg ttacgaatga tctcaatcac ttcgtcgatg
    42001 ttgagaaacg ccaccagcaa accttccagg atatgcaggc gcttgaggac tttctccaga
    42061 cgatagttca gtcggcggcg cacggtatcg cgacggaaca ccagccattc ggagaggatt
    42121 tccagcaggt ttttcaccgc cggacgacca tccagaccga tcatattgag gttaatgcga
    42181 tagctctttt ccagatcggt ggtagcgaag aggtggttca tcacctgatc catatccacg
    42241 cggttggaac gcggcacaat caccagacgg gtcgggttct cgtggtcaga ttcatcgcgc
    42301 agatcgtcaa ccatcggcag ctttttgttg cgcatttgcg cagcaatttg ctccagtacg
    42361 cgcgcacctg aaacctgatg cggtaatgcg ctgataacca ccgcgccatc ttctttcttc
    42421 cacaccgcgc gcatacgcac tgaaccacgt ccgttctcgt agattttacg aatctcggcg
    42481 cgcgaagtga taatttccgc ttcagtcgga taatccggcc cctgcacgat atccagcagc
    42541 tgatcgagcg tagttttcgg ctggtcgatt aatgcgattg ccgcctgagc tacttcacgc
    42601 aggttatgcg gtggaatatc ggtcgccatg ccgacggcaa taccggtggt gccgttaagc
    42661 aaaatgtttg gcagacgggc aggtagcatt ttcggctcct gcaaagtacc gtcgaagttt
    42721 ggcacccagt cagccgtccc ctgccccagc tcgctcaata gcagctcgga atatttcgac
    42781 aaccgggatt cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg cgcgccccag
    42841 ttcccctgac catcaaccag cggataacgg taagagaacg gctgcgccat caggaccatc
    42901 gcttcataac aggcgctatc gccgtgcgga tggtatttac ccagtacgtc accgacggta
    42961 cgggccgatt ttttaaattt ggcgctggca ttcaggccca gttcagacat cgcatacaca
    43021 atgcggcgct gaacaggttt cagaccatca ccaataaacg gcaacgcacg gtccatgatc
    43081 acgtacatgg agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc aaggcgctct
    43141 gccatatcgc tcattaattc tgattcctca acttattcgc cagccttcaa aggggcaata
    43201 ttgccggaga tactacatta tctgccgcgt cgagtcacag gagatcagcg tgaatagcgc
    43261 cgccggacgt gctccgacgg cgataaaggc atcaatgtgc cttgatatac aactctttgc
    43321 tgtagacttg atcggcatag agggcagcac aaggttgcgc ccatgccaca ccactcggca
    43381 tgggggtccg ctccgggcga ttcgagaagt tgaggttatg cgagtccagg gaagcctaaa
    43441 gcatcaaagt agtcgactgt aagtatacgg ttcagcccag tttagctgtg tctccaccag
    43501 tgccccgcgc ctcctgccat aagtgcggct tgctcatgga tatccccgag acggcttacg
    43561 gcgcaccgga taataaattt cgcgtataca tggtattcct taccggagaa attataagcc
    43621 caacaacaat acgcctgtga cattgttcgc tcaaacttat cgcgagtgat ttggctcaca
    43681 ctccggtaaa gattccccat cacgccaacg gcacataaac atccgtttcc agtcgctgct
    43741 ctgtcacccc ttcggcaaga ttggtgtagt gaaacagaat cggtgcttta cgcattttct
    43801 cgccgcttgc aggcagccag tggcgaataa tgccccatat cgtgtgtgaa atatcgtcca
    43861 gctcgccaac gtggcgggcc acggcataac gtccaccggt aagttcacca ttgctcacac
    43921 cataacgatt atcgggaatc ggttcgctaa cgctgccgca gatatcaaag cgaaacgctt
    43981 ccggtgcggt ggtggcaggg gcgtcccagg cgacgccaaa agtctggctt tggttaacag
    44041 gagaaagccc cgtctcttta cgccacatga taaatttcgc cacactgtag ttgagcaact
    44101 cagggctaca gcggtgcggc aacatcgcca cagggattga tgggaaatcg ataatgttta
    44161 catccagtgt caggtttgtc attttgcctc cctgcgaaat tattcccggg aacgcgtccc
    44221 gggagcggta acaattacgg atttactttg cggatctgtt tgacgtcaat ttcaacagaa
    44281 ttccagtctt tatcgacttc accctgaatc tcaaccgtat ctttcggcgt caccgtcaca
    44341 ccgttccagc gtttgtgatc gatatcaaca ttgatagtac cgctggcatc tttaaacacg
    44401 tagagatcgt cagagatgcg ttcaacgata ttgccgcgca gggttaccca ggtgtcgtca
    44461 cgcagggatt ttgcgctttc tacagtcgtt acgctgccgt tcggcccctg aaatcctccg
    44521 gcctgacttt gcgttaccga tgggccagaa aaaccgccct gctctgctgc catcaccggt
    44581 gcactgcaca gggccattac tgcgattact gctgcgaatt ttttcatgtt tattactccc
    44641 tttaatgtct gtttccgagc atttaacaag atagtcctta acaacttctt aagggaaaaa
    44701 aataaaattt aatgctgtac agagcgcgtt acaacacggt ttactggcag caaatacggt
    44761 tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag atgacatgct gattggtgac
    44821 ggcatcaaaa ctggccttag taaaatgggt tttagtgtcg actggtttac acaaggtcgt
    44881 cagggaaaag aggcgcttta tagcgcacct tatgatgcgg tgatcctgga tttaacctta
    44941 ccaggcatgg atggtcgcga tattttgcgc gaatggcgag agaaaggtca gcgtgagccg
    45001 gtactgaacc tgaccgcgcg tgatgcgtta gcggaacgtg tagaagggct gcgtctggga
    45061 gctgacgatt atctgtgtaa accttttgcg ttgatagaag tcgccgccag gctggaggct
    45121 ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc gccacggtaa cgtcatgctc
    45181 gaccccggca aacgtatcgc cacgctggct ggcgaaccct taacactgaa accaaaagaa
    45241 tttgccctgc tggaattact gatgcgtaac gctggtcggg tactgccgcg caaactgatt
    45301 gaagagaaac tgtatacctg ggacgaagag gtcaccagta atgccgttga agtgcatgtg
    45361 catcatctgc gacgcaaact cggtagtgat tttattcgta ccgtgcatgg tattggctac
    45421 acattaggtg agaaatgaaa tttacccaac gtcttagtct gcgcgtcagg ctgacgctaa
    45481 tctttttaat tctggcctcg gtgacctggc tgctttccag ctttgtcgcc tggaaacaaa
    45541 caacggataa cgtcgatgaa ttgttcgaca cccaactgat gctgtttgcc aaacggttaa
    45601 gtacgctcga tctcaacgaa atcaacgcgg cggatcgcat ggcacagacg ccaaataaat
    45661 taaaacacgg tcatgttgat gacgatgcgc tgacctttgc catctttacc cacgacggca
    45721 gaatggtcct taatgatggc gataacggag aagatattcc ctatagctat caacgggaag
    45781 gttttgctga cgggcaactg gtcggtgaag acgatccttg gcgttttgtc tggatgacct
    45841 cacctgatgg caaatatcgc atcgttgttg gccaggaatg ggaataccgt gaagacatgg
    45901 cgctggcgat tgttgccggg caattgatcc cgtggctggt cgcactgccg attatgttaa
    45961 tcatcatgat ggtactactg ggtcgtgaac tcgcgccgct gaacaaactg gcgctggcac
    46021 tacgtatgcg tgaccctgac tcggaaaaac cactaaacgc gactggcgta cccagcgaag
    46081 tgcggccact ggttgagtcg ctaaatcaac tgttcgcccg cacacatgcg atgatggttc
    46141 gagaacgacg ctttacctcc gatgcagctc acgaactacg tagcccgtta acagcgctga
    46201 aagtgcaaac cgaagttgcg cagctctctg acgatgatcc gcaggcgcgg aaaaaagcac
    46261 tgctccaatt acattccggg atcgatcgcg ctactcgtct ggttgatcaa ctgctcacgc
    46321 tatcgcggct ggactcactg gataaccttc aggacgtcgc ggagatcccg cttgaagatc
    46381 tcctacaatc gtcggtgatg gatatttacc acacggcgca gcaggcgaaa attgacgtgc
    46441 gactgacact caatgcccac ggcatcaaac gcaccggaca accgctattg ctaagtttgt
    46501 tggtgcgaaa tttgctggat aacggccccg gtgtgacacc agaggcactg gcgcgaattg
    46561 gcgaacgctt ctatcgccca cccggacaaa ccgctaccgg cagtggactt gggctatcga
    46621 ttgtccagcg aatcgccaag ctgcatgaca tgaatgttga gtttgggaat gcggaacaag
    46681 gtggatttga ggcgaaggta agctggtaac ttctctgtct atgagagccg ttaaaacgac
    46741 tctcatagat tttactaata gcaaaatata aaccgtcccc aaaaaagcca ccaaccacaa
    46801 accagacaaa taacgagcga gaatacatat agccaaaact cgaacatttc ccgcataaag
    46861 agttttctta agataagaat aataagtggc gtaagaagaa aaaatgctgc aatcattgct
    46921 tgcaatatac gatcttcgcc tgaaacggaa ggtatactgc aaatattgcc tcccgactca
    46981 agcatccagt catacttatt gaaagcaaaa aaagaatcta ttaaccaata agtaagcatt
    47041 atatatagca atgcttcaaa tattgtttta attttctgct ttaacatata tcagtacgct
    47101 cataaaaatc catttataat tatcaacatg acacgttgat attttcatct tttacttatt
    47161 caaagccata ttttcaatcc ccaaaaaaac cgaaccaaac gtgtaaatta ttccggtgcg
    47221 agtaagaatt atgaggaatg gctatcagta ttgtcatttt cagaaaatat ttatcctgca
    47281 tcggtgagtc agagctagat cagacttttg ctaaattcgc aaaagacttt gcacattttg
    47341 ctaatttcac agtaccgctc tgtgacgtac tatagtcgac aaacgtctca ccttgagatt
    47401 aaaaaatgag caacatcctg attatcaacg gcgcgaaaaa attcgcccac tccaatggtc
    47461 aactgaacga caccctgacc gaagtcgcgg atggcacact gcgcgacctc gggcatgatg
    47521 tccgcatcgt tcgcgccgac agcgactaca atgtcaaagc ggaagtacaa aactttctct
    47581 gggctgatgt ggtgatctgg cagatgccag gctggtggat gggcgcgccg tggacagtga
    47641 aaaaatatat tgatgatgta ttcaccgaag gtcacgggac tctgtatgcc agcgatggtc
    47701 gtacccgcaa agatccgtcg aaaaaatacg gttccggcag cctggtacag ggcaaaaaat
    47761 atatgctttc tctaacctgg aacgcaccaa tggaagcctt caccgaaaaa gatcagttct
    47821 tccacggcgt tggcgttgac ggtgtgtatc tgccgttcca taaagcaaac caattcctcg
    47881 gtatggaacc gctgccgaca tttatcgcta atgacgtgat aaaaatgcct gatgttcccc
    47941 gctatactga agaatatcgc aagcatcttg tggaaatttt tggttaacta gagctcaggc
    48001 tttagaagga gttaaccatg cttaccgtaa tcgcagaaat ccgtactcgt cctggtcaac
    48061 atcaccgtca ggcggtattg gatcagtttg ctaaaatcgt tccaaccgta ctgaaagaag
    48121 aaggttgcca cggctatgcg ccaatggtgg attgcgctgc tggcgtgagt ttccagtcta
    48181 tggcaccgga ttctatcgtg atgattgagc agtgggaaag catcgcgcac cttgaagcgc
    48241 atctgcaaac cccgcacatg aaggcgtaca gcgaagccgt aaaaggcgac gtgctggaga
    48301 tgaatatccg tattctgcag ccagggattt aatcctgcct tgtttgcccg gccatcctga
    48361 ccgggcaatg ttctttcctt taaacctcaa tctccgccat gtcgcctttc tcttgcaacc
    48421 agttgcggcg atcttccgag cgtttcttcg ccagcagcat atccatcatc gcgtcagtac
    48481 gctgatcgtc ttcatcatcg atagtcaact gcaccagacg gcgagtgttc ggatcaagcg
    48541 tggtttcacg caattgcatc gggttcattt cccccagacc tttaaaacgc tggacgttcg
    48601 gcttgccttt cttgcgtttt aattgctcaa gtacgccctc tttctcttct tccgtcagcg
    48661 cgtaataaac ctctttcccg agatcaatac ggtagagcgg tggcagtgcg acgtaaacgt
    48721 gaccgtgttt caccaacgcg cggaagtgtt ttacgaacaa agcgcagagc agcgtggcaa
    48781 tgtgcagacc atcagagtcc gcatccgcga ggatacagat tttgccataa cgaagctggc
    48841 tcagatcgtc gctgtcagga tcgataccga tcgctaccga aatatcatgc acttcctgcg
    48901 aggccagcac ttcgtcggaa gaaacttccc aggtgttaag gatcttacct ttcagcggca
    48961 tgatcgcctg atattcacga tcgcgcgcct gcttggcaga tccgcctgcg gagtcacctt
    49021 ccacgaggaa cagttcggta cggttaaggc cctgcgcggt acaatcagcc agtttgccag
    49081 gcagcgccgg gccgctggtc agctttttac gcaccacttt tttagccgca cgcatacgac
    49141 gctgggcgct ggaaatcgcc atctctgcca gcaactccgc cgcctgaacg ttctggttca
    49201 gccacaggat gaaggcatct ttcaccacgc ccgaaacgaa tgccgcacac tgacgcgaag
    49261 agagacgctc tttggtctgc ccggcaaact gcggatcctg cattttaacc gacagaacat
    49321 aggcgcagcg atcccagata tcttccgccg acagcttcac gccgcgcggc agaatgttgc
    49381 ggtattcaca gaactcacgc atcgcgtcca acaggccctg acgcagacca ttaacatggg
    49441 taccgccctg catcgttggg ataaggttga cgtagctttc ggtcagcagt tcaccgcctt
    49501 ccggcagcca cagtagcgcc cagtccacag cttcagtatc gccagcgaaa ttaccgataa
    49561 acggtttttc tggcagcgtc ggtaaaccgt ttaccgcttc cgccaggtaa tcattcagac
    49621 cgtcctgata gcaccagcgc tgttcggtgt tgttgatctc atctttaaag gtaatctcaa
    49681 cgccggggca cagtactgat ttggccttca gcacatgcgt caggcgtgaa acagaaaaac
    49741 gcgggctgtc aaagaaggtt tcatccggcc agaagtgcac gctggtaccg gtattgcgtt
    49801 taccgcaagt gccgacaacc tgtaaatcct gcaccttttc gccattttca aaggcgatgt
    49861 tataaacctg accatcgcgg cgcacgttaa cttctacgcg cttcgacaag gcgttaacta
    49921 ccgaaatccc cacgccatgc aggccgccag agaactggta atttttgtta gagaatttac
    49981 cgcctgcatg cagacggcaa agaatcagtt caaccgccgg tactccttct tccgggtgaa
    50041 tatccaccgg catcccgcgc ccatcgtcaa tcacttctaa cgactggtca gcatgtaaga
    50101 ttacatccac gcgttttgcg tgacccgcca gtgcttcatc gacactgtta tcaataactt
    50161 cttgccccaa atggttaggg cgagtggtat cggtatacat ccccggacgg cggcgaaccg
    50221 gctcaagccc ggtgagtacc tcaatggcat cagcgttata agtttgcgtc atggtttaag
    50281 ttagcaattc gagttgatcg tcagagatgg tgcagaccaa gaaaatcgac gatcgggttg
    50341 aaataatctt cgaagcccgt gaatgcgtgg ttgccgcctt ctatgacagt ctggcggcag
    50401 gaagcgtagt acgccaccgc ctggcggtaa tccagcactt catctcccgt ctgttgcagc
    50461 agccagatca aatccggcgc ttccagcggg tcaatctgca tgactttgag atcgtaaata
    50521 tggcgtgact ctagcacata ttgctgcccg gtgtaggggt tctcgttctg accgagatag
    50581 tccgtcagca attcaaacgg gcgtaccgcc gggtttacca ccactgcggg cagcataaaa
    50641 cattgcgaca accaggtggc gtaatatccc cccagtgacg aaccgacaat acccagcgaa
    50701 tcgccgccat gttccaggac aatggattcc agcagttctg ccgcgtcgga aggatacggc
    50761 ggcaactgcg gaatgatcat ctcaacgtca gggtgatgtt ccgccagcca gttttttaac
    50821 aagctcgctt ttgcagagcg cggcgagctg ttgaaaccgt gtaaataaag aagcgtagac
    50881 atcagtagcc ttctgaagcg gtatcaggtt ggaaacgtgt gtccgccagg cgatgcacct
    50941 cggtggtcag cgtgccatca gcatgtaact cgagagtacg ccagccgggc gcgatagtat
    51001 ccagcgtaaa gttggaacag tgcggcttaa actgcacaca ggtcgacggc gttgccagca
    51061 ggcggcgacc gttccagtcg agatccagct cctgatgaat atgaccgcac agcaagtatt
    51121 tgacgtgcgg aaacttcgcc agcacggtat ccagttcgcc cgcgttacgc agactgtgtt
    51181 gatcgagcca actacaaccc gcaggtagcg gatgatgatg cagcagcagc aacgtatggc
    51241 gttctggcgc atcggccagt ttacgttcca gccactcaag ctgaaactcg ctcagctcac
    51301 cgtgcggcac gccaaacacc tggctatcca gcaacaggat ttgccattgc tcaccaataa
    51361 acacccgctt cgccggggag atacccgcat cctgtaacgc gctgtacatc gcgggctgga
    51421 aatcgtggtt gcccggcagc cagacgcagg gcgcacgaaa acttgcgatg ccttcagcga
    51481 aatgctgata ggccgcagag gattgatcct gcgctaaatc acctgtcgcg acaatcaggt
    51541 cgaattcgtg ctggtgtgga cgaatcgcct ccagcaccgc ctggtagctt tcccaggtgt
    51601 ttacccctaa cagggcttcg tgcttttgtg caaacaggtg agtatcggta atttgtaaaa
    51661 tccttcctct ggcctcacca gccagaggaa gggttaacag gctttccaaa tggtgtcctt
    51721 aggtttcacg acgctagtaa accggaatcg ccatcgctcc atgtgctaaa cagtatcgca
    51781 accagtccgc taaaaactgg ttaatttgat gcttttcgtc gcgttgatgc aactttttat
    51841 taggataatc ataccgcgct ttgaagcgaa aaatctgctg gcttgaacac acttcagcca
    51901 ccatcgcgtc atgatacaga cgcaccgtca ttgacggaag gctccagtaa ctgatcgcgg
    51961 gcgcagtctg ttctattgtc accagggtag tgtatcgggt cgattccaca atcgtcagcc
    52021 gatattgtgc gtttgccacc tgatagctta cagtttcgcc gggtgcgtca ttgcgcggta
    52081 acaaacggcg caattgtgaa aagttcatct cgcacaggcg catcatttca ggaaagtcag
    52141 gtgtgtaacg cttcatttat gcccactcat tttttaacgc ttgatgatgc agctgcagcc
    52201 attgcaaagc gatgaccgac gctgcgttgt cgattttccc ctcttctacc cactggtatg
    52261 cctgttcccg gcttaccaca tgaacgcgaa tatcttcgtt ttcatcagcc agaccatgaa
    52321 taccgcttgc ggtcgtggcg tccacttcgc ccaccataat tgacgaacgc tcactggtgc
    52381 cccccgggct tgccaggaaa cttaacaccg gtttggtccg tttgactatc agtcccgcct
    52441 cttcaatcgc ttcgcgacgg gcaacatctt ccacactttc accctcttca atcatcccgg
    52501 caaccatctc cagtagccag ggggtttcgc tggtgtcgta cgcggcaatc cgaatctgct
    52561 caatcagcac aacttcatca cgcactgggt caaagggtag caagactgcg gcgtgaccgc
    52621 gctcaaaaat ttcccgccgt acctcatgac tcatttgccc gttgaataga cgatgacgaa
    52681 atctataaag atctaatgaa aaaaagccgc gataaagtgt ttctcgtgca ataatttcta
    52741 catcgttttt gccaaatgta acgggcaggt tgtctggctt aagcattgtt aatgtcctag
    52801 cactaatagt gaattaaatg tgaatttcag cgacgtttga ctgccgtttg agcagtcata
    52861 tgttaaattg aggcacatta acgccctatg gcacgtaacg ccaacctttt gcggtagcgg
    52921 cttctgctag aatccgcaat aattttacag tttgatcgcg ctaaatactg cttcaccaca
    52981 aggaatgcaa atgaagaaat tgctccccat tcttatcggc ctgagccttt ctgggttcag
    53041 ttcgttgagc caggccgaga acctgatgca agtttatcag caagcacgcc ttagtaaccc
    53101 ggaattgcgt aagtctgccg ccgatcgtga tgctgccttt gaaaaaatta atgaagcgcg
    53161 cagtccatta ctgccacagc taggtttagg tgcagattac acctatagca acggctaccg
    53221 cgacgcgaac ggcatcaact cgaacgcgac cagtgcgtcc ctgcagttaa ctcaatccat
    53281 ttttgatatg tcgaaatggc gtgcgttaac gctgcaggaa aaagcagcag ggattcagga
    53341 cgtcacatat cagaccgatc agcaaacctt gatcctcaac accgcgaccg cttatttcaa
    53401 cgtgttgaat gctattgacg ttctttccta tacacaggca caaaaagaag cgatctaccg
    53461 tcaattagat caaaccaccc aacgttttaa cgtgggcctg gtagcgatca ccgacgtgca
    53521 gaacgcccgc gcgcagtacg ataccgtgct ggcgaacgaa gtgaccgcac gtaataacct
    53581 tgataacgcg gtagagcagc tgcgccagat caccggtaac tactatccgg aactggcggc
    53641 gctgaatgtc gaaaacttta aaaccgacaa accacagccg gttaacgcgc tgctgaaaga
    53701 agccgaaaaa cgcaacctgt cgctgttaca ggcacgcttg agccaggacc tggagcgcga
    53761 gcaaattcgc caggcgcagg atggtcactt accgactctg gatttaacgg cttctaccgg
    53821 gatttctgac acctcttata gcggttcgaa aacccgtggt gccgctggta cccagtatga
    53881 cgatagcaat atgggccaga acaaagttgg cctgagcttc tcgctgccga tttatcaggg
    53941 cggaatggtt aactcgcagg tgaaacaggc acagtacaac tttgttggtg ccagtgagca
    54001 actggaaagc gcacatcgta gcgtcgtgca aaccgtacgt tcctccttca acaacattaa
    54061 tgcttctatc agtagtatta acgcctacaa acaagccgta gtttccgcgc aaagctcatt
    54121 agacgcgatg gaagcgggct actcggtcgg tacgcgtacc attgttgatg tgttggatgc
    54181 gaccaccacg ctgtacaacg ccaagcaaga gctggcgaat gcgcgttata actacctgat
    54241 taatcagctg aatattaagt cagccctggg tacgttgaac gagcaggatt tgctggcact
    54301 gaacaatgcg ctgagcaaac cggtttccac taatccggaa aacgttgccc cgcaaacgcc
    54361 ggaacagaat gctattgctg atggttatgc gcctgatagc ccggcacccg tcgttcagca
    54421 aacatccgca cgcactacca ccagtaacgg tcataaccct ttccgtaact gatgacgacg
    54481 acggggcttc ggccccgtct gaacgtaagg caacgtaaag atacgggtta tctgccgcat
    54541 tcttccccct tctcgcttca atttcgacca gccatcctct attctgatgg gtatttacca
    54601 ctggtcccgg aagacaaaaa tgaaacggac aaaatccata cgccacgcat cgttccgcaa
    54661 aaactggagc gcacgccatc tgacaccagt cgctctcgcg gttgccactg tttttatgct
    54721 ggctggctgt gaaaagagtg atgaaacagt gtctctctat caaaatgctg acgactgttc
    54781 agctgcaaac ccaggcaaaa gcgccgaatg taccaccgcg tacaacaatg cgctgaaaga
    54841 agccgaacgt actgcgccga aatacgccac ccgtgaagac tgtgttgctg aatttggtga
    54901 aggtcagtgt cagcaagcac cagcccaggc tggcatggca ccagaaaacc aggcgcaggc
    54961 tcagcaatcc agcgggagtt tctggatgcc gctgatggcc ggttacatga tggggcgtct
    55021 gatgggcggc ggcgcgggat ttgcacagca gccgctgttc tcctcgaaaa acccggccag
    55081 tccggcttac ggtaaatata ccgacgcgac gggtaaaaac tatggcgcag cccagccagg
    55141 ccgcaccatg accgtaccga agacggcaat ggcaccaaaa ccggcgacca ccactaccgt
    55201 tacccgtggc ggttttggtg aatctgttgc caaacaaagc actatgcagc gtagcgcaac
    55261 cggcacctct tctcgttcaa tgggtggctg ataccgatgg aaagagtcag tattaccgag
    55321 cgcccggact ggcgtgagaa agcccacgaa tacggtttca attttcacac catgtacggc
    55381 gagccgtact ggtgtgaaga tgcttactac aagttgaccc tcgcccaggt tgaaaagctg
    55441 gaagaagtca ccgccgaact gcaccagatg tgcctgaaag tggtggaaaa agtgatcgcc
    55501 agcgatgagc tgatgaccaa attccgcata ccaaaacaca cctggagttt tgtgcgtcaa
    55561 tcatggctga ctcaccagcc atcgctttat tcgcgtcttg atctggcgtg ggatggcact
    55621 ggcgaaccta aacttctgga aaataacgcc gatacgccaa cgtcactata cgaggcggcg
    55681 ttctttcagt ggatctggct ggaagatcag cttaacgccg gtaacttgtc ggagggcagc
    55741 gaccagttta acagtctgca agaaaaactg atcgatcgct tcgttgagct gcgtgaacag
    55801 tatggcttcc agttgctgca tctcacctgc tgtcgcgaca cggtggaaga tcgcggaacc
    55861 attcagtatt tgcaggactg cgcaaccgaa gctgaaatcg ccaccgagtt cctctacatc
    55921 gacgagatag gtttaggcga aaaaggccag ttcacggatt tacaggatca ggtgatttct
    55981 aacctgttca agctttatcc gtgggaattt atgctgcgcg aaatgttctc caccaagctg
    56041 gaagatgctg gcgtacgctg gctggaaccg gcgtggaaga gcattatctc caacaaggca
    56101 cttctaccgc tactgtggga gatgttcccg aatcacccga acctgctgcc cgcttatttt
    56161 gcggaagatg atcatccgca aatggaaaaa tatgtggtta aaccgatctt ctcccgtgaa
    56221 ggcgcaaacg tgtcgatcat tgagaacggc aaaaccattg aagcagcgga aggtccgtat
    56281 ggcgaagaag ggatgattgt tcagcaattc cacccgttac cgaaattcgg cgacagctat
    56341 atgctgattg gtagctggct ggtgaacgat caacctgccg gaattggcat tcgtgaagac
    56401 cgtgcgttga tcacccagga tatgtctcgg ttttatccac atatttttgt tgaataagcc
    56461 acgataccgg atggcactcg ccatccggta attgttagcc tatctgcacc gacagcatac
    56521 ttaaactgcc catttctata ccatcgaccg gaatggtaat tggctcctgc ccatcccaca
    56581 cacctaacac atacaacaac ggcagatagt gctctggcgt tgggttcgat aacgcgccac
    56641 cttcatggtc gagataattc accagaggat gctgctctac cggcccttgc cacgtcagat
    56701 tcgctttcac atactcatta aacgacgtcg cccacggata cggtgaacta tcaccgtgcc
    56761 acttcactgt gcgcaggtta tgcaccacgt taccgctggc gaccaacatt attccttcat
    56821 cacgcagcgc cgcgagtttg cgccccattt cgaaatgcca ggcggcaggt ttgctactgt
    56881 cgatactcaa ctgcaccata gggatatcgg cgtcaggata catcttaatc agcacgcccc
    56941 acgagccgtg gtcaaagccc caggcttctt tatccagcgt caccgggatc ggcgctaaca
    57001 gctcaaccag acgctgtgcc agcgcaggcg aacccggagc aggataatgc gtatcgtaca
    57061 gcgcctgcgg gaagccacca aagtcatgaa tcgtgggcgg cgtctccatc gcggtcactc
    57121 ctgttccacg ggtaaaccag tgagccgaaa ccaccacaat cgcttgcggg cgtggcaatg
    57181 tcatccccaa cttctgccag ctgcgggtat acaaattatc ttccagcacg ttcatcggac
    57241 taccgtgacc taaaaacaat gctggcatac gtgttgaaga catgatgata tccttaacta
    57301 aaggtgtcat tttgatatcc tcacaatacg cttgttcggc ggagtaagaa cccggataac
    57361 aatgatgatg atcatcagtt attttgacga tctgcctgaa ggtgaagatt tataaggagt
    57421 tgtcgatgtc agtacctctc attctgacca tactggcggg ggcagccacg tttattggcg
    57481 cgtttctcgg cgttctcgga caaaaaccct cgaaccgctt actggcgttt tcgctaggtt
    57541 ttgcggcggg gatcatgttg ctcatctcat taatggaaat gcttcctgcc gcactagcag
    57601 ctgaaggaat gtcgccggtg ttgggttatg gaatgtttat cttcggtctg cttggctact
    57661 ttggcctgga ccgcatgttg ccgcatgcgc atccgcagga tttaatgcaa aaatcggtgc
    57721 agctgttgcc aaaatcgatc aagcgcacag ccattctgct cactctcggc atcagtctgc
    57781 ataacttccc ggaagggatt gccacctttg tcacggcgag cagcaacctg gagctgggat
    57841 ttggcatcgc actggccgtc gcgttgcaca atatccctga aggtctggca gtggcaggcc
    57901 cggtttatgc ggcaacgggg tctaaacgca ccgcgattct gtgggcgggg atttctggac
    57961 tagcagaaat tcttggtggt gtgctggcgt ggttaatcct cggtagcatg atttccccgg
    58021 tggtcatggc ggcaatcatg gcggcggttg caggaattat ggtggcgctc tcggttgatg
    58081 aattaatgcc gctcgccaaa gagattgacc ctaataataa ccccagctat ggcgtactat
    58141 gtggaatgtc agtgatggga ttcagtttag tgctgctaca aacggcggga attggttaaa
    58201 aacaacatca gaagttgtcc agcacatgga caacttctgc cagcttcacg aaagccttat
    58261 tctacctttt cggctgtctc ttctctcgta ctgtttaagt atttgttccg cgcttcaagc
    58321 agtgcgtctc tgtcctggcg aaacgcccga caataacgtt tcaaatgcag gaaataaccc
    58381 accacaacca gagcaatcaa tacaatccag taccaggcga taaacatcct tttaccctta
    58441 atatttaaca atactaaaca tttagcgcat aaatttcaca tatccttttt cggatatatt
    58501 catatggtcg cataataacg gaacaattat atgattttga tcataatgaa aaagaatgcc
    58561 tgatattaat gaaattttct catagaaaga gaagcggaat taacatcttg catagcaggg
    58621 caaaataaaa accgaagcca gctacaactt cggtttcttg attaaggcag taattaagca
    58681 gcggttttca gctggcttta cgctcatgtg cctgacggta tgccaccagg tcttcaatag
    58741 tcacgagcgc catattgtgt ttattggcaa actcaatgca ctctggtgca cgcgccatcg
    58801 tgccatcgtc attagtcagc tcacacagca caccagccgg tttaaagcct gccagcgtca
    58861 tcaaatcaat agtcgcttca gtatgaccgc cacgcgtcag tacaccacct gcctgcgcgc
    58921 gaagtgggaa aacgtggccc gggcgattca gatcagaagg ttttgcgcca tcggcaatcg
    58981 ctgcgcgaac ggtcgtaata cgatcagcgg cagaaacacc ggtagtcaca ccttcagccg
    59041 cttcaatggt cacggtaaaa ccggtgccat aggcgctggt gttattttct accatcattg
    59101 gcagatcaag ttgtttacgg cgatcttcag tgatgcacag gcaaacaata ccgctaccgt
    59161 ggcgaatggt cagcgccatc tgctcaacgg tcatggtttc tgccgggaag atcatatcgc
    59221 cttcgttttc acggtcttca tcatcaagca ccattacacc gcgtccttca cgcagcgcag
    59281 ccagtgcatt ttcaacacgt tcgaaaggcg taccaaaaga ggaaagtagc gtctgattca
    59341 tggtaaaaaa acctcactaa aattatggtt accagaatca gggcagtctt aggagtggcg
    59401 gcatatagcc aaaataacgt gagcgggtcc atgcccgaca gaatcgttac tctctcccat
    59461 ccggactcta accgtcggcc ccggaattac accggatctg ctgtcctttg agttcgcacc
    59521 caaagcgctc gcgggctttc aactgagttg atttaccgcc ggtggggaat ttcgccccgc
    59581 cctgagaata agcggattca ctataacgct aatgattagc ggcagcaacg catagcttca
    59641 cataattctg gtttatgact tacccttatc gcactacaat ggtactcaac acctatcagt
    59701 acagggaaac tacaatgatt gacccgaaaa aaattgagca aatcgctcgc caggttcacg
    59761 aatcaatgcc taaaggaatc agggagttcg gggaagatgt ggagaaaaaa atccgccaaa
    59821 ccctacaagc tcagctgacg cgcctcgacc ttgtaagccg tgaagagttc gacgtccaaa
    59881 cgcaagtcct gttacggact cgtgagaaac tggcgctgct tgaacaaaga atcagcgaac
    59941 tggaaaatcg cagtactgag atcaaaaagc agccagatcc agagactctc cccccaacgc
    60001 tgtaaacatt tgtctttaat gcactgtaaa ttatgaatta atcctctaat ttacagtgtt
    60061 ttctattaca ttatcgctta atcccattca gagtatcatc acaaaaatga atgattgcaa
    60121 ttgaaaatat aattgttatg tattctttaa gttttactta atcgatattt agactggccc
//
LOCUS       DAOFMX010000013        59234 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.013, whole genome shotgun sequence.
ACCESSION   DAOFMX010000013 DAOFMX010000000
VERSION     DAOFMX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.013"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="QBA20_000950"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="QBA20_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDQ6291587.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPV
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="QBA20_000951"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="QBA20_000951"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDQ6291588.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4112
                     /gene="xni"
                     /locus_tag="QBA20_000952"
                     /pseudo
     CDS             3358..4112
                     /gene="xni"
                     /locus_tag="QBA20_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
     gene            complement(4165..5313)
                     /gene="fucO"
                     /locus_tag="QBA20_000953"
     CDS             complement(4165..5313)
                     /gene="fucO"
                     /locus_tag="QBA20_000953"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDQ6291589.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5341..5988)
                     /gene="fucA"
                     /locus_tag="QBA20_000954"
     CDS             complement(5341..5988)
                     /gene="fucA"
                     /locus_tag="QBA20_000954"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDQ6291590.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6535..7851
                     /gene="fucP"
                     /locus_tag="QBA20_000955"
     CDS             6535..7851
                     /gene="fucP"
                     /locus_tag="QBA20_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDQ6291591.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7884..9659
                     /gene="fucI"
                     /locus_tag="QBA20_000956"
     CDS             7884..9659
                     /gene="fucI"
                     /locus_tag="QBA20_000956"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDQ6291592.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9768..10584
                     /gene="fucK"
                     /locus_tag="QBA20_000957"
                     /pseudo
     CDS             9768..10584
                     /gene="fucK"
                     /locus_tag="QBA20_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            10614..10778
                     /gene="fucU"
                     /locus_tag="QBA20_000958"
                     /pseudo
     CDS             10614..10778
                     /gene="fucU"
                     /locus_tag="QBA20_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="catalyzes the interconversion of alpha-L-fucose to
                     beta-L-fucose; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
     gene            10836..11567
                     /gene="fucR"
                     /locus_tag="QBA20_000959"
     CDS             10836..11567
                     /gene="fucR"
                     /locus_tag="QBA20_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDQ6291593.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(11611..12711)
                     /gene="rlmM"
                     /locus_tag="QBA20_000960"
     CDS             complement(11611..12711)
                     /gene="rlmM"
                     /locus_tag="QBA20_000960"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDQ6291594.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(12704..13099)
                     /locus_tag="QBA20_000961"
     CDS             complement(12704..13099)
                     /locus_tag="QBA20_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDQ6291595.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13118..14035)
                     /gene="gcvA"
                     /locus_tag="QBA20_000962"
     CDS             complement(13118..14035)
                     /gene="gcvA"
                     /locus_tag="QBA20_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDQ6291596.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(14386..14613)
                     /locus_tag="QBA20_000963"
     CDS             complement(14386..14613)
                     /locus_tag="QBA20_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDQ6291597.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            14805..16010
                     /gene="csdA"
                     /locus_tag="QBA20_000964"
     CDS             14805..16010
                     /gene="csdA"
                     /locus_tag="QBA20_000964"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDQ6291598.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16010..16453
                     /gene="csdE"
                     /locus_tag="QBA20_000965"
     CDS             16010..16453
                     /gene="csdE"
                     /locus_tag="QBA20_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDQ6291599.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(16504..17310)
                     /gene="tcdA"
                     /locus_tag="QBA20_000966"
     CDS             complement(16504..17310)
                     /gene="tcdA"
                     /locus_tag="QBA20_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDQ6291600.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(17549..18646)
                     /gene="mltA"
                     /locus_tag="QBA20_000967"
     CDS             complement(17549..18646)
                     /gene="mltA"
                     /locus_tag="QBA20_000967"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDQ6291601.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            18855..18931
                     /locus_tag="QBA20_000968"
     tRNA            18855..18931
                     /locus_tag="QBA20_000968"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18889..18891,aa:Met,seq:cat)
     gene            18965..19041
                     /locus_tag="QBA20_000969"
     tRNA            18965..19041
                     /locus_tag="QBA20_000969"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18999..19001,aa:Met,seq:cat)
     gene            complement(19115..20368)
                     /gene="amiC"
                     /locus_tag="QBA20_000970"
     CDS             complement(19115..20368)
                     /gene="amiC"
                     /locus_tag="QBA20_000970"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDQ6291602.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            20600..21931
                     /gene="argA"
                     /locus_tag="QBA20_000971"
     CDS             20600..21931
                     /gene="argA"
                     /locus_tag="QBA20_000971"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDQ6291603.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(21993..23819)
                     /gene="recD"
                     /locus_tag="QBA20_000972"
     CDS             complement(21993..23819)
                     /gene="recD"
                     /locus_tag="QBA20_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDQ6291604.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRACRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(23819..27361)
                     /gene="recB"
                     /locus_tag="QBA20_000973"
     CDS             complement(23819..27361)
                     /gene="recB"
                     /locus_tag="QBA20_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDQ6291605.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(27354..30242)
                     /gene="ptrA"
                     /locus_tag="QBA20_000974"
     CDS             complement(27354..30242)
                     /gene="ptrA"
                     /locus_tag="QBA20_000974"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDQ6291606.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(30418..33786)
                     /gene="recC"
                     /locus_tag="QBA20_000975"
     CDS             complement(30418..33786)
                     /gene="recC"
                     /locus_tag="QBA20_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDQ6291607.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(33799..34131)
                     /locus_tag="QBA20_000976"
     CDS             complement(33799..34131)
                     /locus_tag="QBA20_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDQ6291608.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(34116..34523)
                     /locus_tag="QBA20_000977"
     CDS             complement(34116..34523)
                     /locus_tag="QBA20_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDQ6291609.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(34520..35083)
                     /locus_tag="QBA20_000978"
     CDS             complement(34520..35083)
                     /locus_tag="QBA20_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDQ6291610.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35074..35544)
                     /locus_tag="QBA20_000979"
                     /pseudo
     CDS             complement(35074..35544)
                     /locus_tag="QBA20_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(35728..36522)
                     /gene="thyA"
                     /locus_tag="QBA20_000980"
     CDS             complement(35728..36522)
                     /gene="thyA"
                     /locus_tag="QBA20_000980"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDQ6291611.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(36529..37404)
                     /gene="lgt"
                     /locus_tag="QBA20_000981"
     CDS             complement(36529..37404)
                     /gene="lgt"
                     /locus_tag="QBA20_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDQ6291612.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(37555..39801)
                     /gene="ptsP"
                     /locus_tag="QBA20_000982"
     CDS             complement(37555..39801)
                     /gene="ptsP"
                     /locus_tag="QBA20_000982"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDQ6291613.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKTRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(39814..40344)
                     /gene="rppH"
                     /locus_tag="QBA20_000983"
     CDS             complement(39814..40344)
                     /gene="rppH"
                     /locus_tag="QBA20_000983"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDQ6291614.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41029..41718
                     /gene="mutH"
                     /locus_tag="QBA20_000984"
     CDS             41029..41718
                     /gene="mutH"
                     /locus_tag="QBA20_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDQ6291615.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            41787..42500
                     /locus_tag="QBA20_000985"
     CDS             41787..42500
                     /locus_tag="QBA20_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDQ6291616.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            42638..42856
                     /gene="ygdR"
                     /locus_tag="QBA20_000986"
     CDS             42638..42856
                     /gene="ygdR"
                     /locus_tag="QBA20_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDQ6291617.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            42964..44004
                     /locus_tag="QBA20_000987"
     CDS             42964..44004
                     /locus_tag="QBA20_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDQ6291618.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44037..45227)
                     /gene="lplT"
                     /locus_tag="QBA20_000988"
     CDS             complement(44037..45227)
                     /gene="lplT"
                     /locus_tag="QBA20_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDQ6291619.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45220..47379)
                     /gene="aas"
                     /locus_tag="QBA20_000989"
     CDS             complement(45220..47379)
                     /gene="aas"
                     /locus_tag="QBA20_000989"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDQ6291620.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            47965..48996
                     /gene="galR"
                     /locus_tag="QBA20_000990"
     CDS             47965..48996
                     /gene="galR"
                     /locus_tag="QBA20_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDQ6291621.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49003..50265)
                     /gene="lysA"
                     /locus_tag="QBA20_000991"
     CDS             complement(49003..50265)
                     /gene="lysA"
                     /locus_tag="QBA20_000991"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDQ6291622.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            50387..51322
                     /gene="lysR"
                     /locus_tag="QBA20_000992"
     CDS             50387..51322
                     /gene="lysR"
                     /locus_tag="QBA20_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDQ6291623.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(51309..52001)
                     /gene="ygeA"
                     /locus_tag="QBA20_000993"
     CDS             complement(51309..52001)
                     /gene="ygeA"
                     /locus_tag="QBA20_000993"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDQ6291624.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52130..53548)
                     /gene="araE"
                     /locus_tag="QBA20_000994"
     CDS             complement(52130..53548)
                     /gene="araE"
                     /locus_tag="QBA20_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDQ6291625.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(53863..54624)
                     /gene="kduD"
                     /locus_tag="QBA20_000995"
     CDS             complement(53863..54624)
                     /gene="kduD"
                     /locus_tag="QBA20_000995"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDQ6291626.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(54654..55490)
                     /gene="kduI"
                     /locus_tag="QBA20_000996"
     CDS             complement(54654..55490)
                     /gene="kduI"
                     /locus_tag="QBA20_000996"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDQ6291627.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(55777..56958)
                     /locus_tag="QBA20_000997"
     CDS             complement(55777..56958)
                     /locus_tag="QBA20_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDQ6291628.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            57213..58442
                     /locus_tag="QBA20_000998"
                     /pseudo
     CDS             57213..58442
                     /locus_tag="QBA20_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
     gene            59077..>59234
                     /locus_tag="QBA20_000999"
     CDS             59077..>59234
                     /locus_tag="QBA20_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6291629.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSK"
BASE COUNT        14632 a        15934 c        14389 g        14279 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 ctggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggta gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt ggcgttcaac
     3841 cgcagcagtt gcccgattac tggggacttg cggggatcag cagttcaaag gtaccgggtg
     3901 ttgcgggaat cggaccaaaa agcgccacgc agctgctggt cgagtttcag agtctggaag
     3961 ggatatatga gaatctggat gcggttgccg aaaagtggcg caaaaaatta gaaacccata
     4021 aagagatggc gtttctgtgc cgcgatattg cccgcttaca aaccgatttg catatcgacg
     4081 gcaatttaca gcaattgcgg ttggtacggt aacggcgagc cggatacgcc gcaaatcgta
     4141 tccggcatta tcacatcagc gcatttacca ggcggtatgg taaagctcta caatatcctc
     4201 aagcgttgct tcacgcgggt tgccaccggt acaaacatca tccagtgccg cctgagccag
     4261 tgccgaaatg tcttccttgc gtacaccaac atcacgcaaa tgtggcggaa taccgacatc
     4321 acggttgagg gcaaacaccg cttcaacagc ggcattacgc gccccttcta ggctcatacc
     4381 ttccactttc acgcccataa cgcgcgcgat atcgcggtac ttctcaccgg taaagtcagc
     4441 gttatagcgc atgacatgcg gtaacaggat ggcgttcgca acaccgtgtg gagtgttata
     4501 aaacgcgccc agtggatgcg ccataccatg caccaaccct aacccaacat tcgagaagcc
     4561 catacccgca acatactgcc cgagcgccat ttcttctccg gcatccttat caccagcaac
     4621 cgatcctcgc agcgccccag caatgatttc aatcgcttta atgtgcagtg catcggttag
     4681 tgcccacgcg ccacgggtaa tatacccctc aatagcatga gtgagcgcat caacacccgt
     4741 cgcggctttc agcgccggag gcataccatc catcatgtca gcatcaataa acgccacctg
     4801 cgggatatca tgcggatcaa cgcaaacaaa cttgcgccgt ttttcttcgt cagtgatcac
     4861 gtagttaatg gtcacttctg ccgcagtacc tgctgtggtg gggattgcca gaatcggtac
     4921 actgggttta ttggtcgggg aaagcccttc caggctacgc acatcggcaa actccgggtt
     4981 gttgctgata atgccaattg ctttacaagt atcctgtgga gaaccaccac caatagcgat
     5041 caggtaatcc gcgccgctat tctggaatac accgagccct tctttgacga cagtaattgt
     5101 tgggttgggc actacgccgt cgtaaatcgc ccatgccagc cctgcagcat ccatcttatc
     5161 ggtcactttc gccaccacgc cgcattgcac cagcgtttta tcggtgacga tcagcgcctt
     5221 ctgataacca cggcgtttca cctcatcggt taaagcccca acagcacccc gaccaaacca
     5281 tgccgtttcg ttcagaatca ttctgttagc catcatcctt ctccttgttg ctttacgaaa
     5341 ttactcttca atccgtaacc cataggtttt gaatttctcc agcactacgg caatctcttc
     5401 atcgctcagc actggcaccg ggtccgtaat cgccagggtc gtcaggtaaa gttgcgccag
     5461 cacttcaact tcatgcgcca gccataacgc tttttccaga ttcacctcac aagcgataag
     5521 cccatgatgt tgtaacaaag ttgccttacg atttttgaga gccagcgcaa catgttcaga
     5581 aagttcgcgt gttccaaagg tcgcataagg cgcgcaagga atagaattac cgccagccgc
     5641 cgcaatcatg tagtgaatag cggggatcga tcggttaaga atggaaactg ccgtgcaatg
     5701 aacggcatga ttgtgaacaa ccgcgttggc atccggtctg ctttgatagg ctgccatatg
     5761 gaaacgccat tcgcttgagg ggagctttcc ttcctcatgt ttaccgttgc catcaataaa
     5821 gacaatatgc gactccgtca gtttttcata tggaatgcct gtaggcgtaa tcagcatccc
     5881 atcctgataa cgtacactga cgttccccgc tgtcccctgg ttcagtccca ggcgggtcat
     5941 ttccaggcaa gtgtcaataa tctgacgagc aagtttattt cgttccatta gctacctctc
     6001 tctgattcaa aacagggcaa taatgttgtt cctttcacac tattgaatta gccgtttaat
     6061 tacccaccat cttcttcctg attaacaaga aagaaattca caagcttata ttttgtgacc
     6121 tagctcaact aatcacagta aataactgca agttctcttt ttataacccc gttaaaaatg
     6181 accgctctta aaaatattta tcaaaacggt catttttcca tttcccctcg ccagaaatgc
     6241 ccgttttcgg cacaaacaat taatacggtc atctggtttg tgttttttat gatttatttt
     6301 ctgaaacggg catgaaattt cgattattaa agtgatggta gtcacataaa gtcaccttcc
     6361 agctaataag tgtgaccgcc gtcatattac agagcgtttt ttatttgaaa atgaatccat
     6421 gagttcattt cagacaggca aatattcacc gatatgaagc ccgaactcgc tggttttgca
     6481 cttttgaaaa cataaccgat tacgtgctta agcttctgaa cctaagagga tgctatggga
     6541 aacacatcaa tacaaacgca gagttaccgt gcggtagata aagatgcagg gcaaagcaga
     6601 agttacatta ttccattcgc gctgctgtgc tcactgtttt ttctttgggc ggtagccaat
     6661 aaccttaacg acattttatt acctcaattc cagcaggctt ttacgctgac aaatttccag
     6721 gctggcctga tccaatcggc cttttacttt ggttatttca ttatcccaat ccctgctggg
     6781 atattgatga aaaaactcag ttataaagca gggattatta ccggattatt tttatatgcc
     6841 ttgggcgctg cattattctg gcccgctgca gaaataatga actacacctt atttttagtt
     6901 ggcctattta ttattgcagc cggattaggt tgtctggaaa ctgccgcaaa cccttttgtt
     6961 acggtattag ggccggaaag cagtagtcac ttccgcttaa atcttgcgca aacatttaac
     7021 tcgtttggcg caattatcgc ggttgtcttt gggcaaagtc ttattttgtc taacgtgcca
     7081 catcaatcgc aagacgttct cgataaaatg tctccagagc aattgagtgc gtataaacac
     7141 agcctggtat tatcggtaca gacaccttat atgatcatcg tggctatcgt gttactggtc
     7201 gccctgctga tcatgctgac gaaattcccg gcattgcaga gtgataatca cagtgacgcc
     7261 aaacaaggat cgttctccgc atcgctttct cgtctggcgc gtattcgtca ctggcgctgg
     7321 gcggtattag cgcaattctg ctatgtcggc gcacaaacgg cctgctggag ctatttgatt
     7381 cgctacgctg tagaagaaat tccaggtatg actgcaggct ttgccgctaa ctatttaacc
     7441 ggaaccatgg tgtgcttctt tattggtcgt ttcaccggta cctggctcat cagtcgcttc
     7501 gcaccacaca aagtcctggc cgcctacgca ttaatcgcta tggcactgtg cctgatctca
     7561 gccttcgctg gcggtcatgt gggcttaata gccctgactt tatgcagcgc ctttatgtcg
     7621 attcagtacc caacaatctt ctcgctgggc attaagaatc tcggccagga caccaaatac
     7681 ggttcgtcct tcatcgttat gaccattatt ggcggcggta ttgtcactcc ggtcatgggt
     7741 tttgtcagtg acgcggcggg caacatcccc actgctgaac tgatccccgc actctgcttc
     7801 gcggtcatct ttatctttgc ccgtttccgt tctcaaacgg caactaactg aacatatttt
     7861 ccgaataaag tgaggaatct gtaatgaaaa aaatcagctt accgaaaatt ggtatccgcc
     7921 cggttattga cggtcgtcgc atgggtgttc gtgagtcgct tgaagaacaa acaatgaata
     7981 tggcgaaagc tacggccgca ctgctgaccg agaaactgcg ccatgcctgc ggagctgccg
     8041 tcgagtgtgt catttccgat acctgtatcg cgggtatggc tgaagccgct gcttgcgaag
     8101 aaaaattcag cagccagaat gtaggcctca ccattacggt aacgccttgc tggtgctatg
     8161 gcagtgaaac catcgacatg gatccaaccc gcccgaaagc catttggggc tttaacggca
     8221 ctgaacgccc cggcgctgtt tacctggcag cggctctggc agctcacagc cagaaaggca
     8281 tcccggcatt ctccatttac ggtcatgacg ttcaggatgc cgatgacaca tcgattcctg
     8341 ccgatgttga agaaaaactg ctgcgctttg cccgcgccgg tttggccgtc gccagcatga
     8401 aaggtaaaag ctatctgtcg ctgggcggcg tttcgatggg tatcgccggt tctattgttg
     8461 atcacaactt ctttgaatcc tggctgggaa tgaaagtcca ggcggtggat atgaccgaac
     8521 tgcgtcgccg tatcgatcag aagatttacg acgaagccga actggaaatg gcactggcct
     8581 gggctgataa aaacttccgc tatggcgaag atgaaaataa caaacagtat caacgcaatg
     8641 ccgagcaaag ccgcgcagtt ctgcgcgaaa gtttactgat ggcgatgtgt atccgcgaca
     8701 tgatgcaagg caacagcaaa ctggccgata ttggtcgcgt ggaagaatca cttggctaca
     8761 acgccatcgc tgcgggcttc caggggcaac gtcactggac cgatcaatat cccaatggtg
     8821 acaccgccga agcgatcctc aacagttcat ttgactggaa tggcgtgcgc gaaccctttg
     8881 tcgtggcgac cgaaaacgac agtcttaacg gcgtggcaat gctaatgggt caccagctca
     8941 ccggcaccgc tcaggtattt gccgatgtgc gtacctactg gtcaccagaa gcaattgagc
     9001 gtgtaacggg gcataaactg gacggactgg cagaacacgg catcatccat ttgatcaact
     9061 caggttctgc tgcgctggac ggttcctgta aacaacgcga cagcgaaggt aacccgacga
     9121 tgaagccgca ctgggaaatc tctcagcaag aggctgacgc ttgcctcgcc gctaccgaat
     9181 ggtgcccggc gatccacgaa tacttccgtg gcggcggtta ctcttcccgc ttccttaccg
     9241 aaggcggcgt cccgttcacc atgactcgtg tcaacatcat caaaggcctg ggaccggtac
     9301 tgcaaatcgc ggaaggctgg agcgtggaat tgccgaagga tgtgcatgac atcctcaaca
     9361 aacgcaccaa ctcaacctgg ccaaccacct ggtttgcacc gcgcctcact ggtaaagggc
     9421 cgtttacgga tgtgtattcg gtaatggcga actggggtgc caaccacgga gttctgacca
     9481 tcggccacgt tggcgcagac tttatcaccc ttgcctccat gctgcgtatc ccggtatgta
     9541 tgcacaacgt tgaagagacc aaagtgtatc gtccttctgc ctgggctgcg cacggcatgg
     9601 atattgaagg ccaggattac cgcgcttgcc agaactacgg tccgttgtac aagcgttaat
     9661 acattacacc ctgatgtgat gcccggtcgc tccggctacc gggcctgaac aagcaagagt
     9721 ggttagccgg ataaacaatg ttatccggct atattgcagg agcgattatg aaacaagaag
     9781 ttatcctggt actcgactgt ggcgcgacca atgtcagggc catcgcggtt aatcggcagg
     9841 gaaaaattgt tgcccgcgcc tcaacgccta atgccagcga tatcgcgatg gaaaacaaca
     9901 cctggcacca gtggtcttta gactgccatt ttgcaacgct ttgctgattg ctgtcggcaa
     9961 atcaatagtg aactgactga atgccacatc cgcggtatcg ccgtcaccac ctttagtgtg
    10021 gatggcgctc tggtagataa gcaaggcaat ctgctctatc cgatcattag ctggaaatgt
    10081 ccgcgaacag cagcggttat ggacaatatt gaacggttaa tctccgcaca gcggttgcag
    10141 gctatttctg gcgtcggagc ctttagtttc aatacgttat ataagttggt gtggttgaaa
    10201 gaaaatcatc cacaactgct ggaacgcgcg cacgcctggc tcttcatttc gtcgctgatt
    10261 aaccaccgtt taaccagcga attcactact gatatcacga tggccggaac cagccagatg
    10321 ctggatatcc agcaacgcga tttcagtccg caaattttac aagccaccgg tattccacgc
    10381 cgactcttcc ctcgtctggt ggaagcgggt gaacagattg gtacgctaca gaacaacgcc
    10441 gcagcaatgc tcggcttacc cgttggcata ccggtgattt ccgcaggtca cgatacccag
    10501 ttcgcccttt ttggcgctgg tgccgaacaa aatgaacccg tgctctcctc cggtacatgg
    10561 gaaattttaa tggttcgcag cgcctaggtt gatacttcgc tgttaagtca gtacgccgtt
    10621 accgtaatgc gctttcacta caagccccgt gtcctgacat catccgcatc aatcgttttg
    10681 cgttttatga acgggcgcaa aaagcctttg cgatcgttat cacaggcgaa cgagcgaagt
    10741 acgggaatat tcttttaaaa aaaggggtaa caccgtaatc tcataccggt acgcccgcat
    10801 gacgcgggcg gttatcgaat gatggggtga aaaatatgaa agcggcacgc cagcaagcga
    10861 tagtcgacct gctgctgaac cataccagcc tgaccacgga agctctctct gaacagctaa
    10921 aggtcagtaa agaaaccatt cgtcgcgatc tcaatgaatt acagacgcag ggtaaaattc
    10981 tgcgcaatca tggacgcgct aaatatatcc accgtcaaaa tcaagacagt ggcgatccct
    11041 ttcacatcag gctgaaaagc cattatgcgc ataaagcaga tatcgcgcgc gaggcgctcg
    11101 cgtggattga agaagggatg gtgatagcct tagacgccag ttcaacttgc tggtatctgg
    11161 cacgccagtt gcctgacatc aacattcagg tcttcaccaa tagccatccg atttgccatg
    11221 aactcggtaa acgcgaacgc attcaactga tcagttccgg cggcacactt gagcgcaaat
    11281 atggctgtta cgtcaatccc tcgctgattt cccaacttaa atcgctggaa atcgatctgt
    11341 ttattttttc ttgtgaaggg atcgatagca gcggcgcact gtgggactcc aatgcgatca
    11401 acgctgatta caaatcgatg ctattaaaac gtgccgcgca atcgttgtta ttgattgata
    11461 aaagtaaatt taatcgttca ggggaagccc gcatcgggca tctggatgag gtaacgcaca
    11521 ttatttctga tgagcgccag gttgcaactt ctttggtaac agcctgacgg aaagggtagc
    11581 aggccggaga cgacgccccg gccttgcctg ttatcgctcg tcgcgacgac cacccaccgc
    11641 agcccagatg cggcggacgt gcaccgtcac ttcttcgcga tcgtgataca actgccgcgc
    11701 ctgaatctga gcatttatgc catgttcatc aagctgtgcc tgaatatacg ccagattgtg
    11761 tgatacttct tcgtagcgtt ttttcatcgg cagtttgaga ttgaaaatgg tttcacggca
    11821 ccagccatta accagccact gcgccatcaa tgccgcaact ttagccggtt tttcaaccat
    11881 atcgcatacc atccaggaga tattgctgcg tgtcggacgg aatttgaaac cgtcttcccg
    11941 cagccacgtc acctgcccgg tatccatcag actttgcgcc atcgggccgt tgtcgacgga
    12001 ataaacccac atgttgcgct tcaccagttg gtaggtccag ccgccagggc aagcgcctaa
    12061 atccaccgcc cacatcccgt tcgccaggcg ttcatcccac tcatccgcag gaataaacac
    12121 atgaaatgcc tcttccagtt tgagcgtgga acgactcggc gcctctgccg gaaatttcag
    12181 gcgcggaatg cccatataga acggcgaatt attgttgctg tatgagtaac cggtatagca
    12241 gcatcctggt gcaataaaga atacatgcac aaccggacgc ttcggcgttt catagttcgc
    12301 cagcacccca gcatcgcgca aggcagcgcg tagcggaaca gtgaacttac ggcagaattt
    12361 cagcaactct ttgctttcgt tggtatcggc aacttcaaca cgcagttcac cgcccttctc
    12421 tactacaccc tgtaacatgc cgacaatggg ggtaatacga tcttctggcg gcaaatgctg
    12481 caggagttcc cccaccacaa accactggcg ggcaaaaatt aatgaactga acggcagctc
    12541 acggattaac ttatcgccat catcaggttg ataacattca taaatgacat aacccgcatt
    12601 ctctttcacg cgggcaaaac cgaaaatttc ccgctggccg gctttatcgg taatttctgc
    12661 ggcgcactct ttttcaaagc ccggacggca cagcaataca accttattca tgacttacgc
    12721 ccttgcgctt taaacggata gcaccaacta acattaacgc ccagcccgca aggaagctca
    12781 cgccgccaac cggtgtgaca aacgcccaca aacgcagatg ggacagcgcc aggcaataaa
    12841 ggctgccgct gaacagcacc gtgcctaacg cgaggaaaac gctactccag taaaaccaga
    12901 tactgatgcg acgctgcatt gccaccgcca gacctaagat cgccagcgta tgaaacgcct
    12961 ggtattcgag accggtttgg atccagccca tctcaacggc ccccatggtt ttacttaaca
    13021 catgcgcacc aaaagcgccc agtgccacaa aaatgaagcc gctaatagcg gcgaaaatca
    13081 gcataaaacg gctggtcatg gtcgtaccct acgtaaatta ttgttcataa cgaaagcgga
    13141 atttttcttg ttcagcagcg gctttcgcca ggatccattg gcgaaaggcg gctattttac
    13201 ccagttctgc ctggctgtca tgacaaacca gataaaaagc atttttactg accagaacat
    13261 cattaaacgg gcaaacaaga cgtccggcct cgatttcaga ttgcgccatc acgttatttg
    13321 ccagcgccac tccctgcccg tgaatagccg cttgcagcac catggcgcta tgactaaaaa
    13381 ttggcccttg ctgaacgttg atatgattaa accccaactg tcgggtatat gtctgccagt
    13441 cacggcgtga cgcatcatgt aataacgtat gtttagccag atcttccggg gtcttcaagg
    13501 gtttttcgcc tgtcagcagt agcggcgaac acaccggcaa taaatattcg gcgtacagtt
    13561 tttccacccg tagccccggc cagttgcccc gaccataaaa tatcgccaca tcaacatcat
    13621 ccgccagctt atcttcctga cgatcaaccg cctggattcg aacgtcaatt cccggataag
    13681 ctgaattaaa gctggaaagt cgcggaacca accaatgaat ggcgaaactg gggagtaaac
    13741 tgaccgtcaa cgcccccttg gcgctacggg cctggagttt acgcgtcgct tcggttaatt
    13801 gcgaaaatat ctctttgata tcgaggaaat agctttgccc ttcctcggtc agcaggagtg
    13861 aacgattacg gcggcggaac agttttagcc ccaaaaaatc ctcaagagac ttgatttgat
    13921 gacttactgc ggcctgggtc acaaaaagct cttctgctgc gcgagtaaaa cttaaatggc
    13981 gtgctgcggc atcaaaaact cgtaaggcat ttagcggtgg taatcgttta gacatggctt
    14041 ttaaactttg atgttaaatg aatttaacaa ttagatcaca ctgtgtaacc tattagtttt
    14101 tttaatctga gccattataa attgtccgtt gagcttctac cagcaaatac ctatagtggc
    14161 ggcacttcct gagccggaac gaaaagtttt atcggaatgc gtattctggt gaacttttgg
    14221 cttacggttg tgatgttgtg ttgttgtgtt tgcaattggt ctgcgattca gaccatggta
    14281 gcaaagctac cttttttcac ttcctgtaca tttaccctgt ctgtccatag tgattaatgt
    14341 agcaccgcct aattgcggtg ctttttttta ccttgcgatc gcgaattact gttccagttc
    14401 gaccatctct ttcacgtcag tacggttgat ctgctgtttg ttgccattag cgtctttata
    14461 cgaaatcata ccgatatcgt tatcagtctg tggtttgccg tcagagacga tggtacgtcc
    14521 gtcattggtg tgcatcacat agttcgaacc ggaacaggcg ctcagggcaa aagtcagcat
    14581 acaggcagaa ataattgcgg cagtcttttt catcatcttc tccttaaaac gattaataaa
    14641 tcttaatagt attgattaac aggctaaaat taacgcctaa cactattcag catatgttac
    14701 ttacgcggct tcgccaggat atccagataa ttctgatggt tagcactctc cttgtatcaa
    14761 agtgaatttt gcgtcacgat cggtgcatca agccgaggag taccatgaac gtttttaatc
    14821 ccgcgcagtt tcgcgcccag tttcccgcac tacaggatgc gggcgtctat ctcgacagcg
    14881 ccgcgaccgc gcttaaacct gaagccgtgg ttgaagccac tcgacagttt tacagcctga
    14941 gcgccggaaa cgtccatcgc agccagtttg ccgaagccca acgcctgacc gcgcgttatg
    15001 aagctgcacg agagaaagtg gcgcaattac tgaatgcacc ggatgataaa actatcgtct
    15061 ggacgcgcgg caccactgaa tccatcaaca tggtggcaca atgctatgcg cgtccgcgtc
    15121 tgcaaccggg cgatgagatt attgtcagcg tggcagaaca ccacgctaac ctcgtcccct
    15181 ggctgatggt cgcccaacaa actagagcca aagtggtgaa attgccgctt aatgcgcagc
    15241 gactgccgga tgtcgatttg ttgccagagc tgattactcc ccgtagtcgg attctggcgt
    15301 tgggtcagat gtcgaacgtg actggcggtt gcccggatct ggcgcgagcg attacctttg
    15361 ctcattcagc cgggatggtg gtgatggttg atggtgctca gggggcggtg catttccccg
    15421 cggatgttca gcaactggat atcgatttct atgctttctc tggtcacaaa ctgtatggcc
    15481 cgacgggtat cggcgtgctg tatggcaaat cagaactgct ggaagcgatg tcgccctggc
    15541 tgggtggcgg caaaatgatt cacgaagtga gttttgacgg tttcaccact caatctgcgc
    15601 cgtggaaact ggaagcagga acgccaaatg tcgctggcgt cataggatta agcgcggcgc
    15661 tggaatggct ggcagattac gatatcaacc aggccgaaag ctggagccgt agcttagcaa
    15721 cgcttgcaga agaagcgctg gcgaaacgtc caggctttcg ttcattccgc tgccaggatt
    15781 ccagcctgct ggcctttgat tttgctggcg ttcatcacag cgatatggtg acgctactgg
    15841 cggagtacgg tattgccttg cgggccgggc aacattgcgc tcagccgcta ctggcagaat
    15901 taggcgtaac cggcacactg cgcgcctcat ttgcgccata taatacaaag agtgatgtgg
    15961 atgcgctggt gaatgccgtt gaccgcgcgc tggaattatt ggtggattaa tgacaaaccc
    16021 gcaattcgcc ggacatccgt tcggcacaac cgtaaccgca gaaacgttac gcaatacctt
    16081 cgcaccgttg tcgcaatggg aagataaata tcgccagttg atcatgctgg ggaaacagct
    16141 tccggctttg ccagacgagt taaaagcgca ggctaaagag attgccggat gcgaaaaccg
    16201 cgtctggctg ggatataccg tggctgaaaa cggcaaaatg cacttctttg gcaacagcga
    16261 agggcgcatt gtgcgcggcc tgttggcggt gttgttgact gccgttgagg ggaaaaccgc
    16321 cgccgaatta caggcacagt cgccgctggc attgtttgat gagctgggat tacgtgcgca
    16381 gcttagcgcc tcacgcagcc aggggttaaa tgcgttaagc gaggcgatta tcgctgcgac
    16441 gaagcaggtt taagcacaga accgggtcgg ataagacgtt cgcgtctcat ccgacctgat
    16501 tgtttaaccc tgacgcgccg ctttcgccat catcttcttc agcgcatgag aaaccgcaac
    16561 aaacccaaag gtggcggtca ccatcgttgc cgcgccaaat ccagatgcac aatccatcct
    16621 cttcggccct tctgccgtgg ctttcatcgc acacaccgta ccgtctgact gcgggtacac
    16681 cagcgcttca gtagaaaaca cgcaatccac gccgagctta cctttactgt ttttcactac
    16741 gccaaaatcg cttttcaggc gctcgcgcaa cttcgccgcc agcggatcct gaatcgtttt
    16801 cgccagatcg gtaacctgaa tctgcgtcgg gtcaatctgc ccacccgcgc caccggtcgt
    16861 gaccagcggg attttattgc gccgacaata agcaatcagc gccgctttgg gccgtacact
    16921 atcaatggca tcaatcacgt acgaataacc ggcactcata tattgcgcta cgttatccgg
    16981 cgtcacgaaa tcatccacca ccgttacacg gcactccggg ttaatctggc gaatgcgctc
    17041 cgccatgact tccgcttttg ccagcccgac gttatcgcgc agagcgtgaa tttgccgatt
    17101 ggtattggtg acgcacacat catccatatc gataagcgtg attgcgccaa tccccgtgcg
    17161 cgccagcgct tccgccgccc aggaaccgac accaccgata ccaaccacac aaatatgcgc
    17221 gtcagcaaac agttgcaacg ctttttcacc atacagacgc gctgtgccac caaaacgctg
    17281 acgccatgca tcactaatta ccacagacat agcacctcag aattaaaaag ggcgaggtta
    17341 tccctcaccc taaacgcata aatgcctgat gcgctacgct tatcaggcct acataactcc
    17401 tgcaatattt tggatttgca tgatttgtag gccggataag gcgttcacgc cgcatccggt
    17461 atgaacaacg cgcacttagt caataatcag gatgtgaggt tacccctcac cctgtcatat
    17521 ccgtaaaaac ggcatacaga atatcacatc agccgctaaa gacgttacct gcgcccggag
    17581 ccgttttcag cacccaaacc cgtccatagt ggttgtacca acctgcgcgg tgtccggctt
    17641 ccggcccgat cccttgatag atatcgaagt gttggccttt gattgcgccc ccgacatcca
    17701 acgccaccat cagacgcaat tcgtactgac cgttaaattt gccgttatta tccagcagcg
    17761 gcacttctgc cagcaaggta gtacctggcg gaataattga acgatcggag gcaacagaag
    17821 cacggccaac cagcggcacc gcacttgccc ctttcaccgg tgcaaaagat tgcggtttaa
    17881 agaagacgaa agacgggttc tgttccagca gctcgcgaac ctcggcttca ctgtgtgttt
    17941 cgccccaatg acgaatcgcc tgcatcgaca tatcttcttt tttcacttcg ccacggtcga
    18001 tcagcacctt accaatgctg cgataggcat gaccgttttt ccctgcatag ctgaaaaagt
    18061 taagcggact gccatcacca aagtcgatat acccactacc ctgcacatcc ataatgaagt
    18121 tatccatcag agagttgctg taagcgagga tgtatttatc gcccaatgcc ccggcgtaga
    18181 tctccgcacg agacggcaga cgaccacgtt ttggcggcat acggtaaata ggatactgga
    18241 actcgccctg gcgggtatgg cgcgcctgaa ttaccggcgt gtaatagcct gtaaactgca
    18301 cgttaccata gttgtcggca ccttccatct gccaggcatc aatgccgaac tggcgcatat
    18361 tgcgggtatc accgcctgcg cgcagccact cttgcaccgc gttataaaca ttactctggt
    18421 tgccatacag acgcggtgac gaattacgga tatggttaat ttgctcggca aaatcaccgg
    18481 cgttaatcgg cgcgccaacg gcatctggct ggttcaccag agagaaaggc tgggtaaatt
    18541 tcccgtcttt atattgctgt ccgcgatcgg ttggtttgga agagcaggcg gcaagcattg
    18601 ccacaaccgt gcccataaga aggtacttta cccaacgtcc tttcattgtt ctcttcttca
    18661 ggttaaaaaa taaggcgcaa cgaagataac aaaccgccgc gtttaatgaa atgagcagcc
    18721 tctccctgac gcaaattttg cacaaaaaat aggctttagt ggtttgtttt tgttcaaaat
    18781 catgctaaat ccgtgatcgg ggtaaaaaaa aggttgcatg aaaacgcgag cggagtatag
    18841 tgcgcatcca cggacgcggg gtggagcagc ctggtagctc gtcgggctca taacccgaag
    18901 gtcgtcggtt caaatccggc ccccgcaacc aatcaaattt gatgaagtaa aagcagtacg
    18961 gtgacgcggg gtggagcagc ctggtagctc gtcgggctca taacccgaag gtcgtcggtt
    19021 caaatccggc ccccgcaacc aattattgaa caccctaacg ggtgtttttt tgtttctgat
    19081 ctcccataaa aaagcgccat tcagcgcctt tttatcatcc ccttctcgcc agcgttgccc
    19141 catcggcaaa atacgcttta atccccgcaa gaatagactc cgcaacttcc tgctggaaag
    19201 tcgccgtttt cagtttacgc tcttcctcaa cgttactgat aaacgccgtt tcgaccagaa
    19261 tggagggaat atctggtgcc tttagtacgg caaacccggc ctgttcaact tgatttttat
    19321 gcagcttgtt gatttttccg agcttattca gtaccgcttt accaaactta aggctgtcag
    19381 caatagtcag cgactgcacc atatcgaaca tggtgtggtc gacatagcgg tcaccgcttt
    19441 tgctcacgcc accaatcaag tccgaggcgt tctgggtttg tgccagatat tttgccgcag
    19501 tactggttgc gcctttggtt gagagcgcaa acaccgagga accgctcggc tgacgactgg
    19561 taaaggcgtc ggcatggata gagacaaaca ggtcagcacg ctgtttctgg gcttttgcta
    19621 cgcgcacttg caacggaatg aagatgtctt cattgcgcgt catgtacacc ttcatattgc
    19681 cctcttcctc gatcagagag cgcaaacggc gagctatttg caataccaca tctttttcgc
    19741 gcgttttgta tttccccacc gcaccggagt cttcgccacc gtggccaggg tcaagcataa
    19801 tgacaatcgg acgatcgcgc cctgctttac ccggttgtgg accactttgc gctggcggca
    19861 cctgcttttc gaggtcgcct ttgttgtaat cctccagcag cgccagcagc gggtcctgca
    19921 tatcctgtgc attggccgga tagaggtcca tcaccagacg ctctttaaac ccggcgaccg
    19981 gcgcaagggc aaacagctgc ggttttacgt tttgctttaa ttcaaaaacc atgcgtacgg
    20041 tttgcgggtc aaattgcccg acgcgcgccg acttgatgaa cgggtcgtct gcacgaattt
    20101 gtgccgccat ccccttgagt accgagttca ggtttacatc ttcgatatcc accaccacgc
    20161 gttcagggtt actcaacgcg aactgcttat atttcagctg acgatttgat tctaccgtca
    20221 cgcgggtgta gctggacgca ggccagacgc gcaccgccac gacctggcta accgcagcca
    20281 ggctgacctg acttacgctc aatagccaca tagcgcccgc gccttgcagt aaacgacgac
    20341 ggctgattgc agtgttggat cctgacatgc ctctcccgag caaaagaaat ctaagctgtg
    20401 taacaagtaa acgactaatt tgaccggaaa ctttagcgaa agacgcataa tctgtcatct
    20461 aataaacggt aaacattctt tttatattca cggcattact gataaaaaag tcgctctcgc
    20521 ataaaattta cacttgcacc ctgcgaaaaa acagaataaa aatacaataa tttcaaataa
    20581 tcatgcaaag gggtgtgccg tggtaaagga acgtaaaacc gagctggtcg agggattccg
    20641 ccattcggtt ccctatatca atacccaccg gggaaaaacg tttgtcatca tgctcggcgg
    20701 tgaagccatt gagcatgaga atttctccag tatcgttaat gatatcgggt tgttgcacag
    20761 cctcggcatc cgtctggtgg tggtctatgg cgcacgtccg cagatcgacg caaatctggc
    20821 tgcgcatcac cacgaaccgc tgtatcacaa gaatatacgt gtgaccgacg ccaaaacact
    20881 ggaactggtg aagcaggctg cgggaacatt gcaactggat attactgctc gcctgtcaat
    20941 gagtctcaat aacacgccgc tgcagggcgc gcatatcaac gtcgttagtg gcaattttat
    21001 tattgcccag ccgctgggcg tcgatgacgg cgtggattac tgccatagcg ggcgtatccg
    21061 gcggattgat gaagacgcgc tccatcgtca actggaaagc ggtgcaatag tgctaatggg
    21121 gccggtcgct gtttcagtca ctggcgagag ctttaatctg acctcggaag agattgccac
    21181 tcaactagcc atcaaactga aagctgaaaa gatgattggt ttttgctctt cccagggcgt
    21241 tactaatgac gacggtgata ttgtctccga acttttccct aacgaagcgc aagcacgggt
    21301 agaagcccag gaagagaaag gcgattacaa ctccggtacg gtgcgctttt tgcgtggcgc
    21361 agtgaaagcc tgccgcagcg gcgtgcgccg ctgtcattta atcagttatc aggaagatgg
    21421 cgcgctgttg caagagttgt tctcacgcga cggtatcggt acgcagattg tgatggaaag
    21481 cgccgagcag attcgtcgcg caacaatcaa cgatattggc ggtattctgg agttgattcg
    21541 cccgctggag cagcaaggta ttctggtgcg ccgttctcgc gagcagctgg agatggaaat
    21601 cgacaaattc accattattc agcgcgataa cacgactatt gcctgcgccg cgctctatcc
    21661 gttcccggaa gagaagattg gggaaatggc ctgtgtggca gttcacccgg attaccgcag
    21721 ttcatcacgg ggcgaggttc tgctggaacg cattgccgct caggcgaagc agagcggctt
    21781 aagcaaattg tttgtgctga ccacgcgcag tattcactgg ttccaggaac gtggatttac
    21841 cccggtggat attgatttac tgcccgagag caaaaagcag ttgtacaact accagcgtaa
    21901 atccaaagtg ttgatggcgg atttagggta agcgatgaaa atcaccggat gcggcaggcg
    21961 taacactcgt gcgtcgcatc cggcaattac gtttattccc gcgaactaaa caacgccgcc
    22021 agaccactgc gccgctcagt acgagtggcg attgccgcac ttaatatacg ctcatcggca
    22081 tacagcgaca gacgacgaca cgcgcgggtc accgcggtgt ataccagctc tcgcgttact
    22141 accggcgtgc gttggctcgg taaaatcaac gccgcatggt cgaactccga tccctgcgat
    22201 ttatgtaccg tcatcgccca cgtagtttcg tgctctggca agcgactcgg ttgcacagac
    22261 ttaatattgc cgtccggcat cgcaaaccag acgcgcgtcc cctgcccgcg atccagcgca
    22321 ataccgatat cgccattaaa caacccaagc gcgctgtcat tacgggcaat catcaccggt
    22381 cggccttcgt accaacgaga gtgcggatga cgatgaattt tgcgcttctg ttgcataaac
    22441 tgttcaattc gctcattcag tccagccacg ccaaacggcc cttcccgcag ggcgcacaaa
    22501 agctggtact cattgaacgc ctgaatgatt aaatccggct cggcacgcgc ttgcagcaga
    22561 tccagataac gtccgtaacc cgcaagagct tcctcaagca tcgcaatata atcttcgccg
    22621 ctctgtaaaa gtcgtttttc gatatcagta aaatcctgct gaaaaacggt tttcactgcc
    22681 gttttatcac cacggttgat cgccgcagct aactgaccaa tgccagaatc gctgccgaaa
    22741 cgatagcttt tttgcagcag gcagagactg tcgcgcaaag atgccgcttc tgtgccggtt
    22801 cctgccggaa cgtgagtccc cgtcaggcgg cttagctgcc tggcacgctc ggcggtaaag
    22861 cccgcgttgg cataagcgca gatatcgccc agcacagccc cagcctcaac cgaggccagt
    22921 tgatcacgat cgccgagaaa gatcactcgc gcatgatctg gcaaggcgtc gatcagtctc
    22981 gacatcatag gcagatcgat cattgacgct tcatctacca ccagcacatc aagatgcagc
    23041 gggttaccgg catgatgacg taaacgctgg ctacccggct gcgcgcccag caatcggtgc
    23101 aaagtgctgg catcttccgg aatgcgtttc ttttgttcat cggtcagcgg taactgtcgc
    23161 aaagccttgc cgagagattc ggttaagcgc gcggcagctt tacccgttgg tgcagccaga
    23221 cggatacggc agcgttcgcc gtcggccatt tgaattaacg ctgccagcaa cttcgccacg
    23281 gtggtcgttt taccggtgcc tgggccgccg gaaatcaccg agatccgccg cgtcagcgcc
    23341 actgccgctg caactttttg ccagttaatt tcatcgctta ctggaaaaag tttgtccagg
    23401 gtttgcgcca gtagagcttc atcaacctca atggtatgat tcacctcatt gaagaagcgc
    23461 gctaccgtcc gctcgttaca ccacatgcga ttcaaataaa gacgatcgcc acagaggatc
    23521 atcggcgtgg gttcatctcc cctgctgacc gcttgagaag ccagcaagca ttcttcccaa
    23581 ttttgtagct caccgatttc actgacacag gtcgccaaca gcggattcga cgcctcgtta
    23641 ttttccagtc gtgaaagcgg caaacagacg tgtccctctc cggcatcatg acttaacagt
    23701 gccgccgcga gggtgacggc aggatgttca tctcccgcca cggtcagggc aaattgcaca
    23761 tccagcgggc gtagctgttt gtgctccaca gcttccagta attgcttttg caatttcatt
    23821 acgcctcctc cagggtcata ccggcaaaca tctcatccat cagggcaatc aacccggcgt
    23881 tgggtcgggt tgtgtaaatc ccttgttgcg gatgttcttt atcaacgcca cgcaggaaca
    23941 gatagataac gccgccaaag tgaagatcat agtcgtaatc agcaatgcga tggcgcagat
    24001 aacgatgcag cgccagggta taaagctgat attgcagatc atagcggtgt gcctgcatcg
    24061 ctgctgccat agcctgttgg gtgtaagccg aactgtcttc acccagccag ttggatttat
    24121 agtcgagcag gtaataacgc ccttcgtggc ggaataccag gtcgataaag ccttttaaca
    24181 tgccgcgtac ctgcatgaac tccagcggcg ggcagccagc ggatagcggg tcaaactggc
    24241 gaattagtgc atcaagctgg ctggcgatga gcggttcgct aatcggcagg taaaactcca
    24301 tctccacctg tttatcgcgg tcggaaagct gactcaggct aacacccgtt tcattgagag
    24361 gtgcctggag gacagccgtg atccactcgg tcaacaccgg ttcccactgc gattcaaagc
    24421 cgccgagttc cagtttttcc tgtacccagt tcgggtcaac cggctgggta aaatcgaggt
    24481 cttcaaacaa actgtgcaag aatgtccccg gtgacgcacc gcgcgggaac tgatgcggtg
    24541 ttaacgtcgg ttcttcaacg acgctgacca cgcctgcggc atcaacatcc agccgtggca
    24601 tcagatcctg agcgataccg tgaccacgct gctgcaagcc ggagtagctg gtgacacgcc
    24661 agttatcgcc gggcaatcgt tgtagtgtcc tcgcattcag ttctgcagtt aaagcatcat
    24721 taacctgcca gggctggtta tcaccagttt gtgccgtttg ccaggcaata tcatcatcgc
    24781 ataacgcttc aatacaggtg cgaagccctg ccgcatcttg cggttcccct ttttgcagca
    24841 aacgcccgag cgcactttgg tggacgtcgg tgtcaccttt tttatcgcca cgacggcgca
    24901 ccagcggtgc aacgccgaga ctgcaatgcc aaaccgaacg cgtcagcgcc acgtaaagca
    24961 aacgcagatc ttccgccaga cgttcgacct ccgcgaggtc gacgctttct ggcgcagcat
    25021 taagatccaa aactgcctca aacgagtggc gatcgtgata aaacgcctga tcctggacgc
    25081 ggaaattggt aataaatggc aaccagacca gcggatattc cagccctttc gatttatgga
    25141 tcgtgacaat ctgcaccaga tgtttatcac tttcgaggcg catttgttgg ctggaggcat
    25201 tactgtctgg ctcgaggata tgttgcgata accagcgtac cagcgcatgt tcactttcca
    25261 gctgcgttcc ggcttcttga agcagttcgc tgatatgcaa gatatcggta agacgccgct
    25321 caccgcctgc cgttgccagc aagttttctg caatgttacg cgccgacatc agcgcccgca
    25381 gcatcggcat aacgccacgt ttgcgccaga tttgccgata accatcgaac tcttcgacta
    25441 ccgcatccca cgcatgttcg tcattattca gcgtttcaat atccagcgca ttcagcccca
    25501 tcattgacgt tgccagcgca ctgcgcaggg tgttctcacg ttcgggcgtc atcaccgcct
    25561 gcaacaacca aagcatttcc tgcgcttcca gagtttcaaa aacactgtcg cggttcgaaa
    25621 ggtaaacgga agggatttcc agcaacgtta aggcatcgcg cacctgggcg gcctcctggc
    25681 ggctgcgcac cagcacgctg atgtccgaag cacgcaccgg acgcgcgtcg tcgccgttca
    25741 tcagcaacgc ttcgccccgc tgtccggctt gtagccagtc gcggatttgc gcagcacata
    25801 cctgcgccat ggtactttga tagtcgccaa cgccgcagct ttcgccttcc atcagccaca
    25861 ttttcatcgc aggctgcgtt tcacctttaa atacaaaacg taacgcctga tttttcccgg
    25921 ctgatttcac tggaataaac ggtatttcgc gaaacatgaa cgtgtcatca gtctggctga
    25981 aaagcttgtt cacactgttc accattccag gtgcggagcg ccagttggtg tctaaagtgt
    26041 agtgggcgtg aacttcacta cgcgccttca tataagtgaa gatatccgca ccccggaatg
    26101 catatatggc ctgcttcggg tcgccaatca gcaacaatgc ggtttccggc tgatggtgcc
    26161 agatacggcg aaaaattcgg tactgctggg ggtcggtatc ctgaaattca tcgatcattg
    26221 ccaccgggaa tcgcgtacgg atcgccgctg ccaacacctc gccgctttcg ctacgcagcg
    26281 cggaatcgag ccgacttaac atgtcatcaa aacccaattc tccacggcgg cgtttttcac
    26341 gcgctactgt ttcgcggatc tcagccaatg cgcgggtgat caacagatcg cggatcgaca
    26401 atggttctgc aagcagttga tcgatcgcct caaacagtgg atgtcgcggg gtttcccccc
    26461 cggccttcgt gcgatcttct aagaaacgct gggagaattt ttccagcgac tccggcaact
    26521 gataactgtt tgtctcttct tctgcccagg cgctgatctt ttcgatccat ttagcctgat
    26581 tgctacggtt aaacttgcgt cgatcaatac cagaagattc gatcagcgca tccagttcac
    26641 ccactgcgtc gcgccactgc tgttttaccg tatcaatacg cgccacaatt tgcgcgtggc
    26701 gggaagccag cgtttcatca tcgggcggcg gtgctttgat aaccggcggt tcgccttgca
    26761 gataacgatt aatatcgcgc agcaacgcct gcggcccttt ccaggtttca aagacgacct
    26821 gggctatttc acgcggcagc gggtagcagt ggcgacgcca gaaatcggcg caggcctggt
    26881 agcgtagcag agactcatct tcaatcagct gctgctcaaa cagcatgccg gattcaaagg
    26941 cattcaggtt gagcatgcgc tggcaaaagc cgtgaatagt aaagactgcc gcttcatcca
    27001 tctgccgttc ggctaacaac aaccactgcg cggcttgcgc tttatcgtcg atctcttcca
    27061 gcaggcgttc gtacagtgga ttgtcggtgg tttcacgcag acaggcgatg cgcaactcgt
    27121 ggatattgct acggatacga ccgcgcaatt ctgccgtggc agcctcggta aaggtgacca
    27181 ccagcagttc ttcaacgatc agcgggcggg gaaaggcggc ggaaccgcct agtccaagta
    27241 acaggcgcaa atagagcgcc gcaatcgtaa aggttttgcc tgtgccggca gaggcttcaa
    27301 tcaggcgctc gccctgtaag ggcaagcgca aaggatctag tgtctcggcg acatcactca
    27361 ttcttttcac tcatcagggg cattgtttgc tgcaacgcgc tgacgttctc ccacactttc
    27421 cagccttcag gatgcacata ttcggctttc ccgttctggc tgccggaaat ctgcgacaga
    27481 atagccatgc cttgcggctc gaccaccgcc tgatggaaga aatcagcaag tttttgcggc
    27541 gtcagcagtt ttatctgggc cacgatttta tcacgcgaat cgaagcgcat attgccgcga
    27601 tcgaaatctt tacttaactt cgatgcttct tcgccgagcg tttgcggtgc ctgcagcatc
    27661 tgggtaatta ccgcctgctg gatttgcgca aactcatctg gcttcatcgc tcgcaatttt
    27721 gcctctgcgg ttgggaaaaa cgccttgtaa cgctcccaca agaatgaagg ctgtttatca
    27781 ttgctttgca aaaggaagcc catgccccac tgacgcccca cgctcattgg aaacgcaaac
    27841 acggcatagc ccaattgttc ttcggtacgc aactgattgt agaaccacgg ctgtacgatc
    27901 tgccccaaca gagagctata ggctgagctg gtgtattcat cgtagccagt cggtacaaat
    27961 accgctgcca gtgcggagtc ggtgctgtta ccggcttttt caaagatgac ggattgtttt
    28021 ttatcgacca ctacatcttt gtttcgacac cactctgaac catcagcgcc caactgtttt
    28081 tgcacatcgc gtgccagcgt tgttgcctgg gcctcggtca tgttgccgat aaccataaac
    28141 tctggtcgag cccctgattt taaggcgtcg cgataggcca gcacctcttt caacgtaatg
    28201 gagggcaaaa ttttacgccg ttcatctcgc gagaagtacg gcacttgcga gagcatctgc
    28261 gcgggcataa tcgcctgctc aaacgcttta cccttttctg cggaatccat catctggtta
    28321 taccaggact tcgcctgctc aagctgctct tccgtagcgg tatagctaaa gtacccctcg
    28381 agcaatgcct ggaacagctg cggcagacgc tgggtgtaac cattagcatt aatcataagg
    28441 ccgttgttag cgttggtgga aaaacttatg ccaccaaccg acgcctggtt gcttaactga
    28501 tcaagcgcca gccctgcgag ataatcattg agcgcaaaca tcacctgatt gcgggcgctg
    28561 tccatggctt tcggattacg caaaatcagg ctgacatcag ctttgggctc gctggcaaaa
    28621 taacggcttg gcgcatacac cacgcgcaga ttcgactcat caacaatcag ctctggatgg
    28681 tcgtatttct tctctgactt aatcagcgag aaatcatcag gaatataagg gttaagctct
    28741 ggcaaagaga gcgcaatgtc ggcggctttt ttctgccagt cggcgaaagt ttgtgcgctg
    28801 attttatcga cctgatacgg cgcatcgaca aagtaagccg ttttgttgtg cggctctttc
    28861 gggctgatat accagatacg cgcattctgc ggcgtcatca tcgccagacg ttcctttact
    28921 gctttagcat cgtaccgatc ggcaatattg actgcatcca gcgtatgctc aacaggaacg
    28981 cgaatcatgg tatctgccag ccattcgacg taatccatat cacgggtgat cgacggataa
    29041 cggaagtcga tatccagcac attcgccagt tcatcgaagt attgtttatc aatgcctttt
    29101 tcacgtaaca gattgagata gctaaaaatt gccgccacaa cctgatcgcg attagccagg
    29161 cctttatcag ttaaagacgc agagatcgct aatacgccgc tgttgccgtt gacgatagga
    29221 tcggagttgg cgctaatgcc ctcaactaat ccctgctttt gcagccagtc agaaagtgta
    29281 cctgggctgc gattgccaat cagataggta atcaattcat cggttttact acggaacttc
    29341 gctgagttgt tatcgatgcg aaactcaacg cgcaacactt tacgcggcag cgcagggacg
    29401 taatgaatga taatgccctt ttgcgcgtcg gtgactaccg gcacggtgat ttccggtttt
    29461 ttgctctctt tgttcggcac gcgaccaaag gtgtccgccg ccatttttgc caactccggc
    29521 agcggtttat tactgtaaat aaccgccttc atcaaattgg cggaatagta cttctcgtgg
    29581 aaatctttca gcgcctgctg caccggatta ccaggtttgt cgcttaaagt ttcgaggtta
    29641 ccaccagaaa actttgaacc ggggtgtgcc gggttaatgg tttctgcgct gacctgtgcc
    29701 atgcgcatcc cgtcacgcgt acgcgccatg gttaattcag cgttcactgc attacgctca
    29761 cgttcggcgt atttcttgtc gagcaagggt tctgcaatag catcggccag gcggtctacc
    29821 gcaccgggca aggcgtcgtt ctcaacttcc agatagaaag ccgtgcgata cggcgcagtg
    29881 ctggcattgt gactaccgcc gtgcattttg agatattcgg ccagactgtc agcctgcggg
    29941 tactttttcg accccatcag actcatatgt tcaaggtaat gtgccagccc ctggtacgcc
    30001 tcgggatctt ccagcgaccc aacgggcact accagcgccg agagcgattt aactgcctgc
    30061 ggatcagaaa ccagcaagac caccataccg ttatccagac gtatagcctg atactggcgg
    30121 ttatctttat cacttttacg gatggtttcc tgaatcggct gccatcccgt ttctgcctga
    30181 cttaatggtg cccagagggc aacaaacaac aataatgctt tgaaccaggt gctgcggggc
    30241 attcacggac ctcataatca actttatttt ctggacagat tcaacaggtt aacgcaaatc
    30301 atctgccaga atttaatctt gtgctgcacg agtcagccta tgtttatata accatcagtc
    30361 cgtgactggt gcgcatcata aagtaagcgg atagattgcg caatttttat acaacactca
    30421 tgactgatta aagcgaaaca gcggtaacag gaaacgttgc gactgttcaa cgatggcctc
    30481 cattgtctct ggtgttaatt gccgccagag ccgttgatac cagatatcat caccttcgcc
    30541 acgcaccatc atgttgcctt cgtaagcctg aaggaatttc gtacgggctt tttgcaacgt
    30601 ggaatcgtca tccagcatgg catcgttttg cgcgtcataa caggttttta gccacgcgcc
    30661 gccactttca ggtaacacca gcaatggcgc ggacattcct tcacgatacc cctcaatcag
    30721 ttgtgagagg taatgcaaag cctgttcggc tgcaagcggc ggaaaacgcc actcaccgtc
    30781 tttgcgtaga aaaaggcgac tttcgccatt accaccgctg gcacagtaga caaggtgttc
    30841 cagccaaagt tgcattccct gcgccacact taataaagag ggacgccagc gcaacaggcc
    30901 atccggctgc acctgcggca accagccagt tatctgcaca ccgttgcagg cgagatcaat
    30961 ttccatactc tgccccggct ggcgacaggc aatgactctg tcggcaagct gctgcatctc
    31021 ctggcactgt gtttcccaga aaatttcacc aaaagcgcca tacggtaaat cccctgccgc
    31081 ccggaagcgg cggaacaagc gttcggcatc atcctgctca accagtgcat tcaataactg
    31141 ctgattgatt tgataacggc taagtccttc cagaataaat ggctcggtgt ctgggatttc
    31201 gctgtcttca gtatggaagt tcacctgcaa acgcatctgg aagaacgccc gcaccggatg
    31261 tgcccagaat cgttgtagcg tttccagcgg cacggtttcc ggtaaggtaa acggcagcgg
    31321 ctgaacaaat tcagaatgtg ctttaccagc ctggctggcc gcaggtagcc attcacgagc
    31381 atagctttgt cgttcgcctg gctggtagtt ttgcggatca aacggcatcc gggtatggag
    31441 gcaagtaaga tgcgctttta cccttgcctc gctttcatca cagttgagcg cttcatcgcc
    31501 cggtagataa tgactttgcc cgatgtagtc gatcagttcc tgcaccagta ccgacgggaa
    31561 acgttcactg ttatcctgaa tggaacgccc gatatagctg atatagagtt tttgctgcgc
    31621 ggaaattaac gcttccagga acaggtagcg gtcgtcatcg cgacggctac ggtcgccacg
    31681 cttcggtttc tggctcatca ggtcaaagcc caatggcgca agctgacgtg gataaacgcc
    31741 gtcgttcatt cccagcaggc aaaccacttt gaacggaatt gaacgcattg gcatcagagt
    31801 acaaatgtta accggtccgg cgagaaaacg ctggctgata cgttcttgat ccagacgctg
    31861 tgccagttca tcacgcaata gtgacagcgg caccgcgtcg ccatactgcg cacctaaacc
    31921 ttcggcgata atcgcctgcc attgttgttc gatcagcgtc atcgccgctt cggtttccgc
    31981 atccggcagg aagaaggcgt tgagcatatc gcgacaaacc ggcaaccact cttctagcgg
    32041 acgctcctgt gccagcccgc gacgccagat gtttagctgc attagcagtg aagccagatg
    32101 ccccaccagt tctgcaatta agccgctcga ttcatcataa ggtagaaccg attgccactc
    32161 gccctgcgcg ctctccatcg cgtagcccag caacatacgc gtcaggccaa atcgccaggt
    32221 gtgttgtccg gtggcgggga gttccagctc gcgaacgttg tcgtcatcta tcccccaacg
    32281 aatgccggat tcgttgaccc actggcgtaa ataacgcagc ccttcttcgg tgatgtcaaa
    32341 ccgcgccgcc agcaccggca catccagcag cgccagcaca tcctctgaca caaagcgact
    32401 gtcaggcagt gataacaggc tgataaacgc ttccagtacc ggatgtgact gccgcgcacg
    32461 acggtcggaa atggcgtaag gcaggtaacg atccgcaggt gcgctaccaa acactgcctg
    32521 aataaacgga ctgtagctgt cgatatcagc caccatcacg atgatgtcgc gcggagtaag
    32581 tgtcggggct tcctccagca tcgccagcaa gcgatcgtgt aaaacttcaa cttcacgctg
    32641 cgggctatgg caaacgtgga atgtgatact gctatccagt ggatcaagcg ggcgtttgtt
    32701 atcgctacgg gaaaactctt cgatgttcac acccgcaacg gcgcggtttt ccagttccag
    32761 aatgtcagac tgaatgttat gcagcaggtt atctggcgtc acatcgacaa aagcgtccag
    32821 ctcctggctg ctctccaggt cagaaaggag ataaatgtag tcgcgcccaa gcttccccca
    32881 tgaggccagc agcgagttgc cgacatcctg ttcaccatcg ctgttaaaga gctgtccggc
    32941 attttcgcta tcgcgaaata gcggcaattc gcgatcttca aaactgtgtc gacgctgacg
    33001 agtcagcagt ttcgccagat aagcaggatc tttaatatcg ccccagtaat aacggcaggg
    33061 gttggtaaac aggagatgga tttcaatatg tttacccagc gcctggagcg cctggagata
    33121 aacaggcggt aacgcggaaa taccgcatat aaagacgcgc gaaggtaacc ccggcgggca
    33181 ggtcgtcgcg gactccagcg tttcgataaa gcgctgatag agattcgcgc ggtgccagcg
    33241 cggttgtccg agttcatggg tatattccac cagcgccttc cacaacgggg cttgccaggc
    33301 ctgtgcttct cccagccctt caaccaaatg tcctgtttcc cactgtgcca gccagtccgg
    33361 acgatagacc agatactggt caaacaggtc cgccgctttt gaggaaagct ggaacagttt
    33421 tcgcttgtcg ctatcgtcag tcagataatg ccgcaacagg gtaaagtctt cgcgctccag
    33481 caattgcggc agcagagtca tcagtttcca gctcatgctc tgtttgttaa aggcgctctc
    33541 tttggggatt tccggtaaca cccggacgaa catatcccag ataaagctcg ctggcagcgg
    33601 aaaatcaatg tttgccgcaa taccaaactt ttgcgacagg gtcatttgca gccactgcgc
    33661 cataccggta ctttgcacca gaatcatctc tggttcgaaa ggatcgtcca gccgttcgcg
    33721 ttcgacaata aactccatca acgcttccag cacgtccaga cgattggaat ggtagaccct
    33781 taacatagcg gctcctgact actgacgatt cgggcaatgc aggcgggtca tctcgccttc
    33841 tctgcccccg ggtgaaacta gcgtaacgct gatgctgaca catcccgcct gcgatgtctg
    33901 cattcggttg acctgccagt tggcaggtgg cgaaatcgcg cgcagttgcg tttgctgcca
    33961 accatgccgc cagagctgtt ggtactggtt tctgctggcg agactgttca ttgatgttcg
    34021 ctggataccc gataacgcag tgacaatcat caccatcagc accatcagca ccatcgcaac
    34081 cattacctcc ggcaggctaa agccttgttg attcttcagg gaagctgaca taatgccccc
    34141 tctttcagcg gacaaaaatc gctccagccg cgtggcgaaa aaacaatttt tccatcaatg
    34201 acttcgcctg ttcgccacaa cgaaacgcct tcataaccgg caatcaataa ggcttcatta
    34261 tctgccagta aacgcaaaca aacctgggca tcggttccag cgtactgcga gcactgaact
    34321 gctggctgcg tctgccagga gtgcattttt ccccacgcca gcgccgactg aacgatggcc
    34381 tggcggcgca atgactgact ttccatgctc acgcgagagg caaaactgcg atcctgctga
    34441 ctcattcctt gcaatagcaa gctacccaaa accagcagca tcaggaccag agccagtgac
    34501 gaaacacctt tttcgcggtt cacaggttga atcctgtcac gctatagctg gcatccacca
    34561 cggtttgcgg ttcagactta ctggcagcac gcatattaac cgtcaacacc ggcgagaagc
    34621 cgctgacatc ctgacgtacg acctgaaaag tgtcgataat gatggcatcc ggattagtga
    34681 ctttatccca gcccttacct tcacaggatg tcgcaccgcg tagcgtttcc agcacatgct
    34741 ccttcagacg aaatccaatc tggtcggact cttttaccgg ttcgcgatcc cagataccgt
    34801 tactgttcgc atcccactgc acaatgatac agtcaccctg tccgacaatt tccagccctt
    34861 cgccggtaca gctgccatga caataacccg ccctctggag atgcttcgcg acggtaaata
    34921 cccgcagcca gatttcatct tccagtgcca gcttacgagt gctcgttaaa ctttcacgct
    34981 gtaacgcagg cagaaagcgt gcagccccca gcaacaatac gctactgatc gccatagcaa
    35041 tcaacacttc cagcagagaa aaaccttgct cttttacagg catccttctg tttctccttg
    35101 ctgataaagc cggagtcttc cccacggcga aaccaccagc caccactcgc ccgttgagtt
    35161 tttgaagcga atatgcccgg cccatgcggt attgcgcagg ccaaagaaag caagcgaagg
    35221 tgtcaggtcg ctcatttcga cttcgggcca gcgtggcaca aagaccaatg gtgaactgcc
    35281 atgacaggta ttggccccag cagcggaact cacaaggcac cataacgtcc cctccctgat
    35341 aacgctgata ctgtggtcgc ggttatgcca gttggcatct tcacgtaaat agagcaaata
    35401 gtcccgcgcc tagctggcgg tttgccaaag ccgttgcgac tgctgccagt attgccagcc
    35461 atagagtcca cttgcgctta gcatgaccaa aatcagcatc gcgaccagcg tttcaatcag
    35521 cgtataacca cgttgtgttt tcatgccggc agtatggagc gaggagaaaa aaagacgagg
    35581 gccagtttct atttcttcgg cgcatcttcc ggactattta cgccgttgca ggacgttgca
    35641 aaatttcggg aaggcgtctc gaagaattta acggagggta aaaaaaccga cgcacactgg
    35701 cgtcggctct ggcaggatgt ttcgtaatta gatagccacc ggcgctttaa cgcccggatg
    35761 tggatcgtag ccttcaatct caaagtcttc gaaacggtag tcgaagatgg attcgggttt
    35821 acgtttgata atcaacttcg gcagcggacg cggttcgcgg cttaattgca gatgagtttg
    35881 atccatatgg ttgctgtaca ggtgcgtatc gccaccggtc cagacaaaat cacccacttc
    35941 cagatcgcac tgctgcgcca tcatatgcac cagtaacgcg tagctggcaa tgttgaacgg
    36001 caggccgagg aagacgtcac aggagcgctg ataaagctgg caagagagtt tgccgtctgc
    36061 cacatagaac tggaagaatg catggcacgg tgccagcgcc attttatcca gttcgcctac
    36121 gttccacgct gaaacaataa tgcggcgcga atccgggtcg tttttcagct ggttcagtac
    36181 cgtagtaatc tggtcaatat gacgaccatc tggcgttggc caggcgcgcc actgtttacc
    36241 atacactggc ccgaggtcgc cattttcatc ggcccattcg tcccagatgg tgacattgtt
    36301 ttcgtgtaga taagcaatgt tagtatcgcc ctgcaggaac cacagcagtt cgtggatgat
    36361 ggaacgcagg tggcaacgtt tggttgtcac cagcgggaat ccatcctgca ggttaaaacg
    36421 catctgatga ccaaaaatgg aaagcgttcc ggttccggta cggtcgtttt tctgtgtgcc
    36481 ttcgtcgagc actttttgca tcagttctaa atactgtttc atggttcctc aggaaacgtg
    36541 ttgctgtggg ctgcgacgat atgcccagac catcatgatc acacccgcga caatcatcgg
    36601 gatggaaaga atttgcccca tgctgatgta ctgcacccag gcaccggtaa actgcgcgtc
    36661 gggctggcgg aaaaactcaa caatgatgcg aaacgcgccg tagccaatca ggaacaagcc
    36721 tgagacagct cccattgggc gcggtttacg aatatacagg ttgaggataa taaacagcac
    36781 cacaccttcc agcagcagtt cgtaaagctg tgatgggtgg cgcggcagca caccgtaagt
    36841 gtcgaaaatg gattgccact gcgggttggt ttgcagcagc aaaatatctt ctgtacggga
    36901 gcctgggaac agcatggcaa acgggaagtt cgggtcaacg cggccccaca attcaccgtt
    36961 aataaagttg cccagacgcc cggcaccaag accaaacgga atgagtggtg cgataaaatc
    37021 agagacctgg aagaaggaac gtttagtacg gcgggcgaag ataatcatca ccacgataac
    37081 gccaatcagg ccgccgtgga aagacatgcc gccgtcccag acacggaaca gatacagcgg
    37141 atcggccata aactgcggga aattgtagaa cagaacataa ccaatacgtc ccccgaggaa
    37201 gacgccgagg aagcccgcat agagtaagtt ttcaacttca tttttggtcc agccactgcc
    37261 cggacgattc gcccgacgtg ttgccagcca cattgcaaaa atgaaaccca ccagatacat
    37321 caggccgtac cagtgaagcg ccacgggtcc tattgagaaa atgaccggat caaactccgg
    37381 aaaatgcaga tagctactgg tcatctgtca ccacaagttc ttgttatttc gctgaaagag
    37441 aacagcgatt gaaatgcgcg ccgcaggttt caggcgctcc aaaggtgcga ataatagcac
    37501 aaggggacct ggctggttgc cggataccgt taaaagatat gtatatgatc cgcgctataa
    37561 ccctccgcga atcagcccgc ccatgccgcg acgctccata aaggctgcaa cctgatgacg
    37621 aacttcggtc gccagttgcg cttccagact acgctgcgca agattttctg cttcggcaaa
    37681 atcaatgcgc cgcagcaggt attttacccg cgctacagaa cgtccgttca tagacagatg
    37741 gcgataccca agcccaatga ggattgccac gcacatggga tcgcccgcca tttcaccgca
    37801 caaacgaaga tcgataccat gtatttccgc ttcccgggcg atcatcgcca gagctcgtaa
    37861 cattgcagga tgaagactgt cgtaaatgtt cgccacccgg gtattgttgc gatcaacggc
    37921 cagaatgtat tgagtcagat cgttggtgcc aacagagatg aaatcgaccc gctttgccag
    37981 atgcggcagc ataaatacca ttgacggcac ttccagcatg atgccgatac gtggtttggg
    38041 aatttcgtaa ccgatcatct cctcgacttc acgtccggca cgttcaatca ggcggcgtgc
    38101 ttcgtcaact tcatcgaggc tggtgaccat cggcaacaga atattcaggt tgcccgtagc
    38161 ggcattagca cgcagcatcg cccgcacctg gatcaagaag atctccggct gatcgagcgt
    38221 aatgcgaatc ccacgccaac ccaggcatgg attctcttcg ctgatcggca tgtaaggcag
    38281 ctgcttatct gctccgacat ccagcgtacg caaggtgacg ggtttatcat tgaacatttg
    38341 cagcatcccc tgatactgcg ccacctgttc ttcttccgac gggaaaccac tttgcagcat
    38401 gaatgggatt tcagtgcgat aaagaccgat gccatcaata cagctgccca gtttttcttc
    38461 atgttccggg cttaaaccag cattgagcat gacttttata cgctcaccgc tttttaactg
    38521 cgcgggtaaa ttgacgtcat cttccgccag acggctaagc tcaatctctt cactaattag
    38581 ccgctgatat tcttgcagca gtaccggctc cggatcgacc agcaattcac cgcgatagcc
    38641 atcaacgatc agcgtcctac gatgcagcac cgaaggctga atatccgcgc ccatcacggt
    38701 agggatcccc agcgcacgta ccatgatcgc agcatgggag ttggctgcgc catctcgcac
    38761 gacaacaccg actaagcgat cctggggcag ctcagcaaga gttgtcgctg acagttcatc
    38821 tgccaccaga atgaaacgtt ccggccaggc gttcggccct tgattagcgt catcaagatg
    38881 aaacagcaat cgctgaccca gcgcacgtaa atcgccagcc cgctctttga gatagttatc
    38941 gcttagcgcg gcaaactgtt cggcaaattt ttcaatgacc gtttttaccg cccactctgc
    39001 caccgagcct ttatcaacct cggcaaacaa ttcgcgacgc agccgggtgt ccgaaagcag
    39061 gtgcgagtaa agatcgaaaa tagccgccgt ttctttttgt gctccggcgg caaagcgttt
    39121 gctgtagcgg cgaaactcgt ttgccgcttc ttccagcgcc ccggtcagtc gttcgcgttc
    39181 cagagccgga tccagcgttg atgcctgata cacctgttcc attaaaggta acgtggcatc
    39241 ctgccagcct tcggcaatcg ccacaccagg tgctgccggt aatgcgcgga ttcgcgtctg
    39301 gcgatattgc ccaaacaggg cagtcaactg cgactgagaa agaatagctg ccatctgggt
    39361 ggcaagcgtc accaggaagg attcttcact ttcgtcatac tggcgcaact ctcgttgctg
    39421 taccaccagt acaccaagca actggcgacg ttgaataatt ggtacgccta aaaacgcgcg
    39481 gaaacgttct tcttttacgg aggggatgta tttgaagctg gggtgctttt gcgcatctgc
    39541 aaggtttatc ggttccgcca gcctgccaac caggccgacg atcccttcat caaacgcgag
    39601 cgttacagtg cgaccgcgtg tttttttcag cccccgggtc gccatcaggt agtaacaacg
    39661 tcgatcatga tcggccaggt agaccgaaca gacctcggta tccatcgcaa gacagatgtc
    39721 ggtaaccaga atatttaacg cctcattcag gcgtggtgcg ctggctacct tttcgactat
    39781 ttcgcgcagg cgagtgagca taattggcgt gacttaacct cttttacgtc gataagcaga
    39841 tgcgttttgt ggttttggcg tattttcctg cagtgacatc accacactcg cgaactcttt
    39901 cattacccta cggtagacat cacgtttaaa tgacaccacc tgtctgaccg gataccagta
    39961 acttacccat cgccagccgt caaactctgg tgtactgctg gtttgcatat tgatttctgc
    40021 atcgccgctt accagctgca agagaaacca tttttgtttt tggccgatac aaaccggctt
    40081 cgtgtcccaa cgcaccaaac gtttcggtaa tttgtagcgc aaccagttac gcgttgaagc
    40141 aaggattcga acgtctttgc ggcttaatcc tacttcttca aacaattcac ggtacatcgc
    40201 ctgctctgcg gattctccgg ggttgattcc gccttgcgga aattgccagg agtgctgacc
    40261 aaatcgccgg gcccacatta cctgcccctg gcgattacaa atcacgatac ctacgtttgg
    40321 gcggtagcca tcgtcatcaa tcaccggact acctcaaaat aaagctttat atacgaatga
    40381 ttgtttcata ctccaggaag acggtaaacc actctctgca gggcattaca cactaataac
    40441 aattgaataa ctcacagtta tgtgcagagt tataaacaga ggaaggggtg gatagccgtt
    40501 tttattcacc ttttctgtgg atagagttgt gaagaactac ggaattacta cgggaaaacc
    40561 cggagcattc tgaataagcc cgatatagaa attttaaaat attcattatt agtcaatgca
    40621 ttacatgtaa tttgagtaat gaaaaagagt aattcgtgac ccaggtcaca cctctcattt
    40681 acgggttggc gaaagatcaa ccaatgccgt atttatccac agaatgtgcc actaagttaa
    40741 gcactgaacc actaaaaact ggagtttcgt cgcacgtcaa ggctgtaaat gaaaacagta
    40801 gtggaggttt ttcacagtta tcccagcttt ctgtggataa catggtgtaa gatcctgttt
    40861 attttcagtg accagatttg gaaaacctat tgcagtgttg cgcaactcga ttaccggcag
    40921 cctaaaaagc aatataaatc agagaattga acaacacatg tggaaaaagt tacactgcga
    40981 atattcggca cataattact gtttgttttt taaccaaggt atcatgacat gtcccaacct
    41041 cgcccactgc tctctcctcc cgaaactgaa gaacagttgt tagcgcaagc acagcaactt
    41101 tctggttata cattgggaga actggcggca cttgccgggc tggttacgcc agagaattta
    41161 aaacgcgata agggctggat tggcgtgtta ctggagatct ggctaggtgc cagcgcaggg
    41221 agtaaacctg agcaagattt tgctgctctg ggcgtggaac ttaaaactat ccctgtggat
    41281 agtcttggtc gtccgctgga aacaacattc gtttgtgttg ccccgttaac gggtaatagc
    41341 ggggtgacct gggaaaccag ccatgtgcgc cacaagctca aacgcgtact gtggataccg
    41401 gttgaaggcg agcgcagcat cccgctggcg cagcgtcgcg taggatcacc gttgctgtgg
    41461 agcccgaatg aagaggaaga ccgacagcta cgcgaagact gggaagaatt aatggatatg
    41521 attgttctcg gtcaggttga gcggatcacc gctcgtcacg gggaatattt acagataaga
    41581 ccgaaagcag cgaatgcgaa agcgcttacc gaagccattg gtgcccgggg cgaacggatt
    41641 ctgacgctgc cacgcggctt ttatttgaag aagaatttca ccagtgcact actggcccgt
    41701 cattttctga tccagtagcc atcgctttga cctgccgctt tccgggcata taattaccgc
    41761 ttcatttttt tggcagggct ttttagatgt tatttgcatg gataaccgat cctaacgctt
    41821 ggcttgcgct cggtacgctg acgctgctgg agatcgttct tgggatcgac aatattattt
    41881 tcctttctct ggtggtggca aagcttccca cagcacaacg tgctcatgcg cgccgtctgg
    41941 ggttggcggg agccatggtt atgcgtctgg cgctgctggc atcaatctct tgggttacgc
    42001 gcctgacaaa tccgcttttt acaatattca gtcaggaaat ttctgcccgt gatttgattc
    42061 tgcttctggg tggcttgttc cttatctgga aagccagcaa ggaaatccac gaatccattg
    42121 aaggtgaaga agaagggctg aaaacacgcg tttcatcatt cctcggcgct atcgtgcaga
    42181 ttatgctgct ggatattatc ttcagcctcg actcggtgat taccgctgtg ggtctgtcag
    42241 atcacctgtt tattatgatg gcagccgtgg tgattgccgt aggcgtgatg atgttcgccg
    42301 cgcgctcgat tggtgatttt gtcgaacgcc atccttcggt aaaaatgctg gcgctctctt
    42361 tcctgattct ggtgggcttt accctgattc tggaaagttt cgacatccac gtaccgaaag
    42421 gttacatcta cttcgcgatg ttcttctcta ttgcggttga aagcctcaac ttgattcgca
    42481 acaaaaagaa tccgctctga tactccgtac gctctcctgc gggagggcgt actcactcct
    42541 tttaccctca cagtttaaga ttttcctgct ttcaagatat ataacgtcgg tttataaaca
    42601 gactattatc ataggtaggc caaatgagga taaccggatg aaaaaatggg cagtaataat
    42661 ttctgcagtc ggactggcgt ttgctgtttc cgggtgttcc agtgattacg tcatggcgac
    42721 caaagatggc cgtatgattt tgaccgatgg aaaacctgaa attgatgatg ataccgggct
    42781 ggtgagttat cacgatcagc aaggtaacgc gatgcaaatt aaccgtgatg atgtttcgca
    42841 aattattgaa cgttaacaaa taaggtcagc atccggctgg ccttaagatt tttctctttc
    42901 ccttttccct tccctctgcc atttttatat tccttatgtc gtgattataa aaaggaaacg
    42961 gctatgcaat atcaccgtat accccacagt tcgctggaag tcagcacgct ggggcttggc
    43021 acgatgacgt ttggtgaaca gaacagcgaa gccgacgccc acgcacaact cgactatgcc
    43081 gtcgctcagg gcattaacct tatcgacgtt gccgaaatgt acccagtacc tccgcgcccc
    43141 gaaacgcaag ggttaaccga aacctacgtc ggcaactggc tggcgaaaca tggcagccgc
    43201 gaaaagttaa ttatcgcctc caaagtgagc ggaccgtcgc gcaataatga caagggcatc
    43261 cgcccggatc aggcgctgga tcggaagaat atccgcgaag cgctgcatga cagcctcaag
    43321 cgcctacaga ctgattacct cgatctttat caggtgcact ggccgcagcg cccgaccaac
    43381 tgcttcggca aactcggtta tagctggacg gattctgcgc ctgcggtttc gctgctggat
    43441 acgctggacg cactggcaga gtaccaacgc gcgggaaaaa ttcgttatat cggcgtgtcg
    43501 aacgaaactg catttggcgt aatgcgctac ctgcatctgg cggacaaaca cgatctgccg
    43561 cgtattgtca ccattcagaa cccttacagt ctgttaaacc gcagttttga agtaggtctg
    43621 gcagaagtca gccagtatga aggggtcgaa ctgctggcct attcgtgcct gggtttcggc
    43681 acgctgaccg ggaaatatct caatggtgca aaacccgctg gcgcacgtaa tacgctcttt
    43741 agtcggttca cccgctatag cggtgagcaa acgcaaaaag ccgtcgcggc gtatgttgat
    43801 atcgccagac gtcatggcct tgatcctgct cagatggcgc tcgcgtttgt acgccgtcaa
    43861 ccgtttgttg ccagcactct gctgggcgca accacgatgg atcagctgaa aactaacatc
    43921 gaaagtttgc atctggagtt aagcgaagac gtattagctg aaattgaagc ggtacatcag
    43981 gtttatactt atcctgcacc ataaagatta acgccggtta aaaccggcgt taaatattaa
    44041 tgacggcgct gccagatcca cagcgccgtt attgccagcg caaatagcgc accgaagcca
    44101 atgccaatgg gcacgaccgg gatgcctacc attaccgcca gcgagtaaat gcccagcatc
    44161 aacaacatgg cgctgttttc gccaaggttt tgtactgcaa tcgcattccc cgccccgacg
    44221 ctttttttac cccgctcctg tagcaacgca ttgagcggaa cgacaaaaaa gccccccagc
    44281 acgccaatca gcatcaacaa ggcataggct ggcagcagct cgtgttgcag ggagaaaatc
    44341 ggtaccacca cgccaatcaa aatcccggct ggcatacagc gtgacacggt ttccagcgta
    44401 accagcttcg ctgccgcacc tgcgccaacc acgataccaa tcgctaccat cgcgttgaga
    44461 taggtcggcg tagcgttatc ggtaatgccc agcgccaccg gtacccacag caccaacagg
    44521 aaacgcagcg tgacacccgc tccccagaat aaactggtgc ccaccagcga aaaacgcgtt
    44581 tcaccattgc accatagcga ggtgcaggca ttcaggaaac tgcgggtcat gttgatgaga
    44641 ttccaggact gccccggacg cgccgccagt ttgggaatgt agatattggc aacgaccgca
    44701 ccaccgtagg ccagtgcgca tgcggccaga gcgaccagga catgccagtc agccagcaca
    44761 ccaccggcta cggaaccgag taaaatcgcc gctatggcag aagcttccat taaaccgtta
    44821 gcttttacta acttactacc cgtggttaat tcgccgagaa taccgtattt cgccggtgaa
    44881 taggctgcag caccaacacc caccagcgta tagccgagaa acggattgat accaaagcag
    44941 atactggctg cgcccagcag cttcaggccg ttggcaaaca tcatcacccg gcctttggcg
    45001 aagctatccg ccacctgccc gacaaacggc gccaaaagaa tgtaagcacc tacaaacacc
    45061 atttgcagga tgggctggct ccactccgga tagaactgcg ctttcagtaa cgccagagtg
    45121 gcaaacagta gcgcgttatc gccaaacgca gagagaaact gcgccacgat aaccgctttc
    45181 atccccttcg accacaacga agtgttagtg tgcactgact cactcatcgt gttgttccgc
    45241 ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg ccgctgccaa gtaatggcat
    45301 ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc ggcacgccgt gctcgcgggc
    45361 atactgttgc aacttatcgc gcgtcagttc gttatctgtg gtgaaaagca ccagtgcctc
    45421 gcctttgctg gcatcgctct taatcgcagt ggcatggact ttatctggcg aaacaccaag
    45481 tgccagttgt tccaccattt ccagcgacac catttcgcct gcaattttgg caaagcgttt
    45541 tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa cgcacaatat cgccagtgtc
    45601 ataccagccg cgctccattt cgccgcgaac attctcggcg gtgggcactt ccagtacacc
    45661 tggcttctcc acccgcagat agccgttcat tatgttcggc cctttcagtt gcaggcgtcc
    45721 gccctcttcg ataccaggga ccgacaacag gcgtgcatcc attcctggta gaatacgccc
    45781 taccgtaccg ggtttcgccg ccatcggtac gttgatagaa acgacaggcg cgcattcggt
    45841 cacaccgtag ccttcaagga tgcgcaggcc aaatttatcc tgccaaagct gtttggtact
    45901 ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga cgatagaagt catacgggtt
    45961 ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtaccg aacaacacgg tgcaactgcg
    46021 gtcatacacc aagtccggca caatgcggta atgcagcggg cttggataaa ggaacacttc
    46081 tgcacctgta agcagtggcg taaacaggcc taccgtcagc ccaaaggagt gaaacagcgg
    46141 taatgccgac ataaagcgat cgttggtggt gaagtcggca atcgttttaa tctgctcgac
    46201 gttcgccaga atgcttttat ggctatggac gacgcctttt ggatggcctt cagaaccgga
    46261 ggtaaagagg atcagcgcct cttcttccgg ctgctgtttt acctgcgcca ggcgcggcat
    46321 cagcaaatga gcgaagatcc atactttgtc ggcagtggtg acatctgctt ttaaatcttc
    46381 cagatagacc cagcgcacct gagtaagttg ctccggcaga tgccagagtt tgcctttatc
    46441 gagaaactgg cgggaagtga agatggtttt gatttcagcc gccgtaatag cactggtcag
    46501 cccttttacc ccggcagtgt agttcatcat tgcgggaatg cggcgacgcg cgatggcccc
    46561 aaaaatcact gccgcactga tgcctgcatt gggcagcatt aagccgatgc gttcgccttc
    46621 aacactgtat ttttcaagga tgcgtccaac aaacagcgtt ttcgtaagca atttgcgata
    46681 ggagtctggg gtaaagttga cgtcttcgac acatttcttc ccggctccga agcggtacat
    46741 tgcactcagt aaagattcgt acagagtttc acgcgggcgc accgccatac gcgcttccat
    46801 cattatttga tgcagcattt cgccagcgat tttgcgacgg tcacgggcac gcggcgcatc
    46861 cggcatcgcc acctgcgttg gaggcaaaat atgcagagta atttgcggga acaagcgacg
    46921 tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc gccccttcaa tacgcacagg
    46981 aataaccgtt gcaccagact tcgccgcgac aaaacccgcg ccatcgtaga ttttcatcag
    47041 cgagcctgta gtggtgatgc gtccttcagg gaaaatcacc actggtcggc cctgttccac
    47101 cagacgtacc agatgtttaa tagccatagg ttgcgtcggg tcgagaggaa caaagtcgat
    47161 aaatgatttc agccaacgca tataccactg ttggcttatt gaggtgtaaa cggcaaacac
    47221 tggacgcaca ggtaaaaaca gtccaagcaa aatgccatca ataaaagaga cgtgattagg
    47281 cgtaattagt acgcgctcgc ccttcagtgc ctgggtgtca cccgtaacgc gaacgcgata
    47341 caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt ccaactccct ttgctctgat
    47401 tcagtaaaag cgaatggagg gagattacac gagataaaga acgcgagcga cagtaaatta
    47461 ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca agagacaggg tacgaagagc
    47521 gtaccgaata atctcaccaa tcaatacctc tgggatcttg attgtggtct gcacgacgac
    47581 tcttcgccag cgagaaaacg caaaggaatg aagggaaatg caacgaggtg tgtaaattgt
    47641 cggttactgt tacagattga tgaccggcaa aaaaaaacct gcgcatctgc gcaggctggt
    47701 gtaattcatg tgctcaacac gaagttgact tcacctatca atacctctgg gatcaccact
    47761 ttagcaacct gaagccaaac gccaccagcg gtcaatcgca acagcgtttc gcaaagtgta
    47821 accaaaggtt tgcaatctct ttttttgtct tacttatttg cgtttggctc acattcccac
    47881 gatgaaaaca cgccacccct tgaaccaacg ggcgttttcc gtaacactga aacaatgtaa
    47941 gcgtttaccc actaaggtat tttcatggcg accataaagg atgtagcccg actggcaggc
    48001 gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac ccaaagccag cgaagcttcc
    48061 cggctggctg tgcatagtgc aatggagtct cttagctatc acccgaacgc caacgcccgt
    48121 gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg ttggtgatgt ttccgatccg
    48181 tttttcggtg caatggtgaa agcggtcgaa caggtggctt atcacaccgg taatttctta
    48241 ttgattggca acggttacca caacgaacaa aaagagcgtc aggctattga gcaactgatc
    48301 cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga tcccggatgc cgatttagcc
    48361 tcattaatga aacaaatgcc cggtatggtg ctgatcaacc gtatcctgcc tggctttgaa
    48421 aaccgttgta ttgctcttga cgatcgttac ggtgcctggc tggcaacgcg tcatttaatt
    48481 cagcaaggtc atacccgcat tggttatctg tgctctaacc actctatttc tgacgccgaa
    48541 gatcgtctgc aagggtatta cgatgccctt gctgaaagtg gtattccggc caatgatcgg
    48601 ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac aggcaatgac cgagcttttg
    48661 ggacgaggaa gaaatttcac tgcggtagcc tgttataacg attcaatggc ggcgggcgcg
    48721 atgggcgttc tcaatgataa tggtattgat gtaccgggtg agatttcgtt aattggcttt
    48781 gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga ccaccgtgcg ttacccaatc
    48841 gtaacgatgg cgacccaggc tgccgaactg gctttggcac tggcggataa tcgccctctc
    48901 ccggaaatta ctaatgtatt tagtccgacg ctggtacgtc gtcattcagt gtcaactccg
    48961 tcgctggagg caagtcatca tgcaaccagc gactaaccgc agttaaagca attccagcgc
    49021 cagtaattct tcgatggtct ggcgacggcg aatcaaccgc gcctgaccat tatcaaacag
    49081 aacttctggt aacagcggac ggctattgta gttggatgac attgatgcgc catatgcccc
    49141 tgtatcatgc agtaccagat aatcacctgc cttcacttcc ggcaaggcgc gggtttcaac
    49201 atttccccct tcctgctggg taaagacatc gcccgattca cataacggcc cggcgacgac
    49261 ggtttccacc gttggcgcgt gttccagaga acgaccatca gctgccaggg cactgatatg
    49321 gtggtaacta ccgtacattg ccgggcgcat cagatcgttg aacccggcat caaccagcac
    49381 aaagtggcgg ctacccattt gtttgacgct ccgcacctga gtaattaata cgccagactg
    49441 cgctaccagg aagcgacccg gttcaatttc cagtttcaca gggtggccca aatggcgagc
    49501 gatttgctca cgcgcggcat tccacagacc ataataatgt tcggtatcaa ccgcctcttc
    49561 accctgttga taaggaatag aaagcccacc gcccgcagaa atagcctgta aatcctgacc
    49621 gaattcgata acctgacgca ccatagcacc acacacctgc tccagatggg cataatcaac
    49681 gccagaacca atgtgcatgt gaatgccgac tagctgcaga tgatgacgtt gtatcacgtc
    49741 cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg ctgttttcgc caccggtatt
    49801 ggttttttgg ctatgtccgt gaccaaaccc cggattaacg cgcagccata cccgatgccc
    49861 tggcgaaacc tggcccagtt ggtcgagcgt atcaacagaa cccgcattca ccggaatttg
    49921 caattcactg acgcgttcaa gcgtcgcctg atcgataaca tctgccgtaa aaacaatatc
    49981 atcggggtgc gtttgcggat tgtaacccgc cgccaacgca cgctctattt cgcctaacga
    50041 gacggaatcc actttcacgc cctgctcacg cattaagcgc aaaatatgaa tattggaaca
    50101 ggctttctgt gcaaagcgca ccacttcaaa ctgtttcagc gctgcaatct gccgacgaat
    50161 aatttgcgca tcgtagaccc acaccgggca gccaaattca gcgggcaaac gcagcagatt
    50221 ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt ggcataacaa actccagatg
    50281 agtgcttttt tatgattacg ccacatcata aaaagaataa aaaatatcga tttatgtcga
    50341 gtctatgcaa aaatgatatg gattaccgga ttgcgagaga gcgctaatgg ccgccgttaa
    50401 cttacgtcat attgaaattt ttcatgcggt aatgaccgcc ggaagcctga ctgaggcagc
    50461 acacctgcta cacacctcac agccaaccgt cagccgcgaa ctggcgcgct ttgagaaggt
    50521 gatcgggctg aaattgtttg agcgcgtacg tggacgatta catcctaccg tgcaaggact
    50581 gcgtctgttt gaagaagtgc aacgatcctg gtacggactg gatcgcattg tcagcgccgc
    50641 agaaagtctg cgcgagtttc gccagggaga actgtctatt gcctgcctgc cggtcttttc
    50701 gcaatctttt ttaccgcagc tcctgcaacc ctttctggca cgttatcccg atgtcagctt
    50761 aaatatcgtg ccccaggaat caccgctact tgaagagtgg ctctcggccc agcgtcatga
    50821 tttaggactc actgagacac tccatacgcc tacgggaaca gaacgtaccg aattactctc
    50881 tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg gcggtaaaaa aggtattaac
    50941 gccggatgat tttcagggtg agaattacat cagcctttcc cgtactgaca gctatcgcca
    51001 gttgctggat cagctattta ctgaacatca ggttaaacga cgcatgatcg tagaaaccca
    51061 cagcgccgcg tcagtctgcg caatggtacg ggcgggggta ggtatttcgg tggttaaccc
    51121 gctcaccgca ctagattatg cggcaagcgg tttagtggtg cggcggttca gtattgcggt
    51181 tccgttcacc gtcagcctga tccgccccct gcaccgcccg tcatcagcgc tggttcaggc
    51241 gtttagtggg catttacaag cggggttacc gaaactggtc acttctcttg acgctatttt
    51301 gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg catggatcgc cgcggtatca
    51361 aacacaggca aaacactgcg ctcttctggc accagtaaac caatttctgt gcagccaaaa
    51421 atgacgccct gtgcgccctg ttctgcaagg cgagcaatca cttgcgcata ataagcgcgt
    51481 gacgcttcgg taaattgccc cagacacagt tcttcaaaaa taatctgatt aattttcgcc
    51541 cgttcatccg cttcaggaat aagacaattg atggaaaatt gttccgtcag ccgcccgcga
    51601 taaaaatcct gttccatggt gtaacgcgta cccagcagcg ccacacgagt cattccggcc
    51661 ccggtaattg cacgtccggt ggcatccgca atgtgtaaga aaggcagagt gcaacgtgac
    51721 tcaatggcat ccgccacttt atgcatcgta ttggtacata acacaatacc ttctgcgccc
    51781 gcccgctgta agccaagcgc cgcctcagcc agaatgtccc cggttttatc ccattccccg
    51841 cgacgctggc actcttctat ttcatgaaaa tcgacgctat gtagcagcat ttgcgcagag
    51901 tgaagcccac caagccgctg tttaatgcct tcatttatca gacgatagta aggaatggtg
    51961 gattcccagc tcattcctcc cagcaaacca attgttttca ttcctctctc ctgatgtgtg
    52021 ttaccgcaac aaggctgaat aaggaacgag acaaacgcct caacggccag gtgcccaatc
    52081 tctattaacg aaaaaagggc cggatgtaca gcacatccgg cccgtgaaat cagacgccga
    52141 tatttctcaa cttctcgcct gccatcagtt tgcgttcgat atgttccagc gtgacatttt
    52201 tggtttccgg aatgagccag aaagtaatac ccacaaacgc aatgttcagc gcagtgtaga
    52261 gccagaacgt accggcagcg ccaatgctat caagcagtgt caggaaggcc gcgccgataa
    52321 tcatattcga cacccagttt gtcgtcgtcg aacaggtaat accgaaatcg cggcatttca
    52381 gcggctgaat ttcagagcac aggatccaca ccactggcgc ggcgctcatc gcataaccgg
    52441 caatacacat catcgtcatg ccaacagaga gccaggacaa gccactggaa gccgtaccgt
    52501 tatcaaactg catcaggcaa tagcccagca ccagagtgcc taacgccatc acgctgaaac
    52561 caattttcag agccggttta cgacctgctt tatctaccgt aaagaccgca atgaaggtcg
    52621 cgaacataaa ggtcagtccc acgaccagag tcgcaatcat ctgttgttct gtggtcgtaa
    52681 agcccgccat tttgaagata cgcggcgcgt agtacatgat gatgttcata ccggtaaact
    52741 gctgcatcgc ctgcaacaac ataccgagaa acacagcacg acggacgtta cggttgatct
    52801 taaacagtgc ccaaccgccc tgttttaact tcaggctttc acgaatttcg ttgagttctt
    52861 ctcgcgcttt ttccgacgta tcgcgcagca tacgcaacac ttcttccgcc tcaatatgac
    52921 gccccttttc cgccagccag cgcgggctat ttggcaggaa gaccaccaga ataatcagca
    52981 gaactgctgg taaagcaaga acccccaaca ttgcgcgcca gttaccgcta taactgaacg
    53041 ctgtatcgga taaaaacgcc agcacgatgc cgagtgtgac catcaactgg tacatactga
    53101 tcatcttacc gcgaacgttt tcacttgcca tttcagaaag atacagagga gcggtgtaag
    53161 acgcgatccc gacagcaatg cccagcacca cacgagcggc gattaacatc tctacgctgg
    53221 tcgcaaaagc ggaccctata gaaccgagta caaacaggat ggcccccgcc atcaggctgt
    53281 atttacgccc caggcggaac gacagccaac cattaaacag cgcaccaatt gctgcgccga
    53341 gcatcatgct actaaccacc cattcctgca aacgactggt cagcacaaag tgatcggtaa
    53401 tgaacggcaa cgctccggcg attacgccga tatcaagacc aaataacaat cctgcgaccg
    53461 cagcagctac cgaaacaaac atattcatac gccgcgtatc acgcaaagaa cgtggcgtta
    53521 aagcagattc cgtattgata gtaaccattt tttcctgcca gcagagagta agacattgtg
    53581 aaaaaatacg tgaacaattc acacaggtgt caggtcggaa acagcataaa tatggattaa
    53641 attgctgcga catgccgtta tgtgatggat attccaattt taaaattaag ttgaattatt
    53701 gagattatta ttaacctcct aattttaaag cagataaaat tcataaagtt cattaattga
    53761 taattgatat ggattatttc ataaccatga tatggattat gatgatctag agatataaaa
    53821 aaccctgcca tgcggcaggg tcataaaagt aagaggaatg aattaacgcg ccagccaacc
    53881 gccatccaca gcaatggcat aaccatttac ataatctgaa gcgctggagg caagaaacac
    53941 taccggcccc atcaggtcac tcggcagtcc ccaacggcca gctggaatgc gatcgagaat
    54001 ttccgcgcta cgttgttcat ctgcccgcag ttgttgggta ttgttggtcg ccatgtaacc
    54061 cggagctatc gcattaacat taatgttgtg tttagcccat tcgtttgcca tcaatcgcgt
    54121 cacacccatc acgccgcttt ttgatgcggt ataagaaggc acacggatcc cgccctggaa
    54181 ggagagcatt gacgcgatat tgataatctt gccgccattg ccttgcgcga taaagtgttt
    54241 cgccgctgcc tgagacatga agaatacgct cttgatattc aggttcatga catcgtccca
    54301 gtctttttcg ctgaactcga gagcgtcttc gcggcgaatc aatccggcgt tattcaccag
    54361 gatatcaata tgaccaaatt cagctaccgc gcgatccagc agtccaggaa tgccatcaat
    54421 ctttcgcaga tcggcggtca ggcttaaaaa acgacgcccc agcgccgtga cctgctcgat
    54481 ggtttcagtc ggttcaacga tgttaatgcc aacaatgtca cagcccgctt gcgccagccc
    54541 caacgccatc ccctggccca gcccagtatc acaaccagtg acgaccgcaa ctttaccttg
    54601 gagagaaaat gcacttaaaa tcataacaac accttattcg tttatgccca caactagcgc
    54661 aaatctttaa cgcccacatg gtccatatca ttaaagacct ggttttcacc gaccatgccc
    54721 cagataaagg tataagcttt ggttccgacg ccggaatgga tcgaccagct cggggagatc
    54781 accgcctgct cgttatgcat cacaatgtga cgcgtttctt gcggctgccc catcatgtgg
    54841 aaaacgcagg cgtcatcatc catattgaaa tagaaataaa cttccatccg gcgctcgtgg
    54901 gtgtgacacg gcatggtgtt ccacaagtta cctggagcca gctccgtcag ccccatactc
    54961 aattgacagg tttccagtac atccgggaca aaatatttgt taatcgtgcg acggttactg
    55021 gtgaggttat cgcctaacgt gactggagat acttcgtccg gtgtgacttt tttggtggga
    55081 tacgtcgtat gcgcgggtgc gcaattgtaa taaaacttcg ccggagtggc ggtatcaata
    55141 ctggcaaaga caacttcttt tgcaccttta ccaacataca gggcgtcgcg gtgaccgatt
    55201 tcatagcatt gaccatcgac agtaatcgta ccggcaccgc caatattgat aacacctaac
    55261 tcgcgacgtt caaggaaata gcttacgccg agttgtttac caacttcccc gccaacggaa
    55321 accgttttag ttatcggcat aatgccgcca acaataattc ggtcaatgtg gctgtaaacc
    55381 atggtgtact catcggcgac aaataccttt tcaaccaaaa attcattgcg cagcccttgg
    55441 gtatccagcg tttttgcgtg cgcaccgtgg atgctctgtc ttacgtccac atcaacctcc
    55501 gataatcagt gaagcgtgaa aatagaaaac gatagtgaaa cactgtttcg ttttctctat
    55561 aaagggatga tatgcccatg ggctagggtt ttcaattaaa aataaaacaa tgttttattt
    55621 ttaattgaag gattaaaaaa agtgcagtgt cgatcacgaa tatgtcatga gaatggaaaa
    55681 atgagggagc ccagaaaatg tatgtcgact tgccagccat cattattcaa cagattaatg
    55741 acggcagata tttatcggta aggaggaaat gcagagctac tcgtcacgtt caatggtcaa
    55801 tgccacgccc tggcccccgc cgatacaaag cgttgccagc cctttgcggg cattacgttt
    55861 caccatttca tgaaccagag aaaccaggat tcggcaaccg gaagcgccta tcgggtgacc
    55921 gagtgcgatc gcgccaccat tgacattgac ccgacgctca tcccactcaa gcatcttgcc
    55981 aaccgaaagc gcctgtgcag caaacgcttc attagcctcg ataagatcga catcagccaa
    56041 ctgccagcct acacgctcca ggcaacggcg ggtcgcatac accggcgcaa ttcccatcaa
    56101 tgccggatct acaccaacgc tggcaaatgc gcggatgcgg gccagcacgg gtaaattcaa
    56161 cgctcgtgct ttggcttcgc tcatcatcat taccgcagct gcgccatcgt ttatggatga
    56221 tgcattcccc gcggtcacag aaccgagact atcaaatgaa ggatttaaac gggctaaacc
    56281 ttctgcactg gcgtcagtgc gtggctgttc atcggtatca acaaccaacg tctgaccgtt
    56341 actttggatc attaccggga cgatctcatc tttaaatcgt ccggcgtcaa tcgccgctcg
    56401 cgctttttgt tgcgagctaa gtgcgtaagc atcctgcaac tgacggctga tgccatattc
    56461 gcgagccaga ttttcggcgg tgacaccaat atgataatca ttgaaggcat cccacaaccc
    56521 atcatgcacc agactgtcaa ccaactggct attgccaagc tgtgcaccgg tgcggctatc
    56581 agtcagaaca tgtggtgcgc ggctcatgtt ttcctggcca ccggcgatga caatatcagc
    56641 ctcgccacac tgtatcgcct gagtagccag atgcagtgct ttaagaccgg aaccgcaaac
    56701 gtcattaatc gtgattgcag aaacgctgtt aggaagacca cctttaatag ccgattgcct
    56761 tgccggattc tgccctgccc ctgcagtcaa cacctgacca agaattactt catccaccgc
    56821 atatgcagga acgccggtac gttctattaa cgctttcacg accagactac caagttccac
    56881 ggcggaatga cccgctaacg caccacgaaa gcagccgata ggtgtccgta acgccccgac
    56941 aatcacaacg tctttcatca caacctcgca ccaattaaca gtgcaatagt aaatgattgt
    57001 taattacagt tatcttaatt gtttaaaaaa agtgattttt atcacaaagg aaatatgcct
    57061 gagcagtagt cagagtcata actggcacgt aaggtttgca accactaacc caccaataga
    57121 tgggtagata gggcgttaat ctcccatact taacttggtt tatggtaaat tgccctccat
    57181 tttgtttaat ttgtagatga tacgttcaga taatgtctaa tatttggtct aaagaagaaa
    57241 ctctgtgaag tttcgcgctc tatggcacag ccgttgatgc aggcacgcta ttccttccta
    57301 ttcagttagg ttcggcaggg gctgtggtcc tgtttattac tgctctggtc gcctggcctt
    57361 taacatattg gccacataaa gccttatgcc agttcatcct ctcatcaaaa acatcagcag
    57421 gtgaagggat aacgggcgcg gtaacacact actatggcaa gaagattggt aatctgatta
    57481 ccacgctgta cttcatcgcc ttttttgtcg tcgtgttgat atatgcagtg gcaattacca
    57541 actcacttac ggaacagctg gcaaagcata tggttattga tcttcgcatc cgtatgttgg
    57601 tgagtctggg tgttgtatta attctgaatc tcatttttct gatgggacgt catgccacta
    57661 ttcgggtaat gggatttttg gtattcccat tgattgccta tttcttattt ctttccattt
    57721 accttgtcgg tagttggcaa cctgatctat taacaaccca ggtagagttc aatcagaata
    57781 cccttcacca gatatggata tcgattcccg tgatggtttt cgcctttagc catacgccca
    57841 ttatttctac gtttgccata gacagacgtg aaaaatatgg cgaacacgct atggataaat
    57901 gcaaaaaaat tatgaaagtc gcttatctca tcatctgcat aagtgtactg ttctttgtct
    57961 ttagctgcct gctttctatt ccatcttcgt atattgaagc ggctaaagaa gaaggggtca
    58021 ccattttatc ggcgctttct atgctgccga acgccccagc atggttgtca atttccggga
    58081 ttattgtcgc agtagttgcg atgtcgaaat cattcctggg tacgtacttt ggcgttattg
    58141 aaggtgccac agaggtcgtc aaaacaacac tacagcaggt tggtgtaaag aaaagtcgtg
    58201 catttaacca cgcactatca attatgttgg tatcgctgat taccttcatt gtttgttgca
    58261 ttaacccgaa cgcgatttcg atgatttacg cgatcagcgg cccgctcatt gccatgatac
    58321 ttttcatcat gcctacgctg tcaacgtatc tcatcccggc gcttaaaccc tggcgttcca
    58381 tcggaaatct gattacgctg atcgtgggta tcctgtgcgt atcggtaatg ttctttagct
    58441 aatatccgtt gtggatggtt caaaattgct acaattttga accattccat ttttcataat
    58501 attcatttgt tattattccg ccctatcact tctctcgtca tatcctagaa ttatcagtta
    58561 gttaaacaca caaagagatc tgaacatctt aaataagact atttaagatg cataacttag
    58621 attcgcaaga tatcttctgg tcattcaaaa acaatttgcg atatagatat tctcatacca
    58681 tgagatagtt atctggacca cgttcgacca tgcaagatta aatcagtgaa acataatcat
    58741 atttaataac gagaaatgca tttttaaata tcttaatcag aattatagca aatacagatt
    58801 aatcgcaatt tttcccagca aaattacaaa atcaaggtgt attgacatca tcaatcacca
    58861 atgaaattat gcaattatat acggataggg aggttcttaa catggggctt tgtagtcgtt
    58921 ataaaagtct tacatgcaat agttgctcaa tgcattgcca aataatgcca gaagagtcac
    58981 cgcgtttgca gtactgcgca aactcgtgtt ttgatcttac ccagcaatag tggacacgcg
    59041 gctaagtgag taaactctca gtcagaggtg actcacatga caaaaacagt atcaaccagt
    59101 aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg aagccctgaa gcttgctgaa
    59161 cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt atgaatcaca gctctacaac
    59221 tggcgcagta aaca
//
LOCUS       DAOFMX010000014        58559 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.014, whole genome shotgun sequence.
ACCESSION   DAOFMX010000014 DAOFMX010000000
VERSION     DAOFMX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.014"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..1100)
                     /locus_tag="QBA20_001000"
     CDS             complement(<1..1100)
                     /locus_tag="QBA20_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDQ6291630.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKFVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="QBA20_001001"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="QBA20_001001"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDQ6291631.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="QBA20_001002"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="QBA20_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDQ6291632.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="QBA20_001003"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="QBA20_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDQ6291633.1"
                     /translation="MAGINLNKKLWSIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="QBA20_001004"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="QBA20_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDQ6291634.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="QBA20_001005"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="QBA20_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDQ6291635.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="QBA20_001006"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="QBA20_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDQ6291636.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="QBA20_001007"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="QBA20_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDQ6291637.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="QBA20_001008"
     CDS             complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="QBA20_001008"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDQ6291638.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="QBA20_001009"
     CDS             complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="QBA20_001009"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDQ6291639.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="QBA20_001010"
     CDS             complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="QBA20_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDQ6291640.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="QBA20_001011"
     CDS             complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="QBA20_001011"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDQ6291641.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10283..11701
                     /gene="puuA"
                     /locus_tag="QBA20_001012"
     CDS             10283..11701
                     /gene="puuA"
                     /locus_tag="QBA20_001012"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDQ6291642.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11850..12014
                     /locus_tag="QBA20_001013"
     CDS             11850..12014
                     /locus_tag="QBA20_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291643.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12004..13389
                     /gene="puuP"
                     /locus_tag="QBA20_001014"
     CDS             12004..13389
                     /gene="puuP"
                     /locus_tag="QBA20_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDQ6291644.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13523..13768
                     /locus_tag="QBA20_001015"
     CDS             13523..13768
                     /locus_tag="QBA20_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDQ6291645.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14081..15724
                     /gene="sapA"
                     /locus_tag="QBA20_001016"
     CDS             14081..15724
                     /gene="sapA"
                     /locus_tag="QBA20_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDQ6291646.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15721..16686
                     /gene="sapB"
                     /locus_tag="QBA20_001017"
     CDS             15721..16686
                     /gene="sapB"
                     /locus_tag="QBA20_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDQ6291647.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16673..17563
                     /gene="sapC"
                     /locus_tag="QBA20_001018"
     CDS             16673..17563
                     /gene="sapC"
                     /locus_tag="QBA20_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDQ6291648.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17563..18555
                     /gene="sapD"
                     /locus_tag="QBA20_001019"
     CDS             17563..18555
                     /gene="sapD"
                     /locus_tag="QBA20_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDQ6291649.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18557..19363
                     /gene="sapF"
                     /locus_tag="QBA20_001020"
     CDS             18557..19363
                     /gene="sapF"
                     /locus_tag="QBA20_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDQ6291650.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19431..19784
                     /locus_tag="QBA20_001021"
     CDS             19431..19784
                     /locus_tag="QBA20_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HDQ6291651.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20151..20939
                     /gene="fabI"
                     /locus_tag="QBA20_001022"
     CDS             20151..20939
                     /gene="fabI"
                     /locus_tag="QBA20_001022"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDQ6291652.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21083..22210
                     /locus_tag="QBA20_001023"
     CDS             21083..22210
                     /locus_tag="QBA20_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDQ6291653.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22278..24212
                     /gene="rnb"
                     /locus_tag="QBA20_001024"
     CDS             22278..24212
                     /gene="rnb"
                     /locus_tag="QBA20_001024"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDQ6291654.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24290..24392)
                     /locus_tag="QBA20_001025"
                     /pseudo
     CDS             complement(24290..24392)
                     /locus_tag="QBA20_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24446..26420
                     /gene="pdeR"
                     /locus_tag="QBA20_001026"
                     /pseudo
     CDS             24446..26420
                     /gene="pdeR"
                     /locus_tag="QBA20_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26568..26741
                     /locus_tag="QBA20_001027"
     CDS             26568..26741
                     /locus_tag="QBA20_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291655.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26831..27580
                     /locus_tag="QBA20_001028"
     CDS             26831..27580
                     /locus_tag="QBA20_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ6291656.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27849..28067
                     /gene="osmB"
                     /locus_tag="QBA20_001029"
     CDS             27849..28067
                     /gene="osmB"
                     /locus_tag="QBA20_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDQ6291657.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28193..28519)
                     /gene="yciH"
                     /locus_tag="QBA20_001030"
     CDS             complement(28193..28519)
                     /gene="yciH"
                     /locus_tag="QBA20_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDQ6291658.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28519..29256)
                     /gene="pyrF"
                     /locus_tag="QBA20_001031"
     CDS             complement(28519..29256)
                     /gene="pyrF"
                     /locus_tag="QBA20_001031"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDQ6291659.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29449..30618)
                     /gene="lapB"
                     /locus_tag="QBA20_001032"
     CDS             complement(29449..30618)
                     /gene="lapB"
                     /locus_tag="QBA20_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDQ6291660.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30625..30933)
                     /gene="lapA"
                     /locus_tag="QBA20_001033"
     CDS             complement(30625..30933)
                     /gene="lapA"
                     /locus_tag="QBA20_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDQ6291661.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31082..31846)
                     /gene="pgpB"
                     /locus_tag="QBA20_001034"
     CDS             complement(31082..31846)
                     /gene="pgpB"
                     /locus_tag="QBA20_001034"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDQ6291662.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32016..32606
                     /gene="ribA"
                     /locus_tag="QBA20_001035"
     CDS             32016..32606
                     /gene="ribA"
                     /locus_tag="QBA20_001035"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDQ6291663.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32670..35345)
                     /gene="acnA"
                     /locus_tag="QBA20_001036"
     CDS             complement(32670..35345)
                     /gene="acnA"
                     /locus_tag="QBA20_001036"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDQ6291664.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35508..35603)
                     /locus_tag="QBA20_001037"
     CDS             complement(35508..35603)
                     /locus_tag="QBA20_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291665.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35717..35884)
                     /locus_tag="QBA20_001038"
     CDS             complement(35717..35884)
                     /locus_tag="QBA20_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291666.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35887..36051)
                     /locus_tag="QBA20_001039"
     CDS             complement(35887..36051)
                     /locus_tag="QBA20_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDQ6291667.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36346..37320)
                     /gene="cysB"
                     /locus_tag="QBA20_001040"
     CDS             complement(36346..37320)
                     /gene="cysB"
                     /locus_tag="QBA20_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDQ6291668.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37530..40127)
                     /gene="topA"
                     /locus_tag="QBA20_001041"
     CDS             complement(37530..40127)
                     /gene="topA"
                     /locus_tag="QBA20_001041"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDQ6291669.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40507..40758
                     /locus_tag="QBA20_001042"
     CDS             40507..40758
                     /locus_tag="QBA20_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDQ6291670.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40794..41843)
                     /gene="sohB"
                     /locus_tag="QBA20_001043"
     CDS             complement(40794..41843)
                     /gene="sohB"
                     /locus_tag="QBA20_001043"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDQ6291671.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42063..42821
                     /locus_tag="QBA20_001044"
     CDS             42063..42821
                     /locus_tag="QBA20_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDQ6291672.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42818..43408
                     /gene="cobO"
                     /locus_tag="QBA20_001045"
     CDS             42818..43408
                     /gene="cobO"
                     /locus_tag="QBA20_001045"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDQ6291673.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43448..44320)
                     /gene="rluB"
                     /locus_tag="QBA20_001046"
     CDS             complement(43448..44320)
                     /gene="rluB"
                     /locus_tag="QBA20_001046"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDQ6291674.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44421..45041)
                     /locus_tag="QBA20_001047"
     CDS             complement(44421..45041)
                     /locus_tag="QBA20_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDQ6291675.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45038..45919)
                     /gene="yciV"
                     /locus_tag="QBA20_001048"
     CDS             complement(45038..45919)
                     /gene="yciV"
                     /locus_tag="QBA20_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDQ6291676.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46057..46101
                     /gene="trpL"
                     /locus_tag="QBA20_001049"
     CDS             46057..46101
                     /gene="trpL"
                     /locus_tag="QBA20_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDQ6291677.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46078..46171
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46193..47755
                     /gene="trpE"
                     /locus_tag="QBA20_001050"
     CDS             46193..47755
                     /gene="trpE"
                     /locus_tag="QBA20_001050"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDQ6291678.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLC
                     AIATAHHAQETF"
     gene            47755..49350
                     /gene="trpD"
                     /locus_tag="QBA20_001051"
     CDS             47755..49350
                     /gene="trpD"
                     /locus_tag="QBA20_001051"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDQ6291679.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49351..50712
                     /gene="trpCF"
                     /locus_tag="QBA20_001052"
     CDS             49351..50712
                     /gene="trpCF"
                     /locus_tag="QBA20_001052"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDQ6291680.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50724..51917
                     /gene="trpB"
                     /locus_tag="QBA20_001053"
     CDS             50724..51917
                     /gene="trpB"
                     /locus_tag="QBA20_001053"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDQ6291681.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51917..52723
                     /gene="trpA"
                     /locus_tag="QBA20_001054"
     CDS             51917..52723
                     /gene="trpA"
                     /locus_tag="QBA20_001054"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDQ6291682.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53104..53283
                     /locus_tag="QBA20_001055"
     CDS             53104..53283
                     /locus_tag="QBA20_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDQ6291683.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53369..53869
                     /locus_tag="QBA20_001056"
     CDS             53369..53869
                     /locus_tag="QBA20_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDQ6291684.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53915..54421
                     /locus_tag="QBA20_001057"
     CDS             53915..54421
                     /locus_tag="QBA20_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDQ6291685.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54481..55119)
                     /gene="ompW"
                     /locus_tag="QBA20_001058"
     CDS             complement(54481..55119)
                     /gene="ompW"
                     /locus_tag="QBA20_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDQ6291686.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55359..55448
                     /locus_tag="QBA20_001059"
                     /pseudo
     CDS             55359..55448
                     /locus_tag="QBA20_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55476..56219
                     /locus_tag="QBA20_001060"
     CDS             55476..56219
                     /locus_tag="QBA20_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDQ6291687.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56249..56788
                     /locus_tag="QBA20_001061"
     CDS             56249..56788
                     /locus_tag="QBA20_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDQ6291688.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56893..57291
                     /gene="yciA"
                     /locus_tag="QBA20_001062"
     CDS             56893..57291
                     /gene="yciA"
                     /locus_tag="QBA20_001062"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDQ6291689.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57331..58047)
                     /gene="tonB"
                     /locus_tag="QBA20_001063"
     CDS             complement(57331..58047)
                     /gene="tonB"
                     /locus_tag="QBA20_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDQ6291690.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPV
                     KKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            58262..58558
                     /locus_tag="QBA20_001064"
     CDS             58262..58558
                     /locus_tag="QBA20_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDQ6291691.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14057 a        15140 c        14643 g        14719 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaatgatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaaatt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgtctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaagaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatactcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc actggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt tcccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgca cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
     8281 gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt agccggagaa
     8341 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
     8401 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
     8461 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcggttttca
     8521 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
     8581 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
     8641 gctgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc attgcgctta
     8701 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     8761 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     8821 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     8881 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8941 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     9001 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     9061 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     9121 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     9181 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     9241 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     9301 tagtggtcag agtcgctgtt tttcagcgat atggtgctgc caagcggtga taaagccctc
     9361 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
     9421 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     9481 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     9541 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     9601 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
     9661 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
     9721 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
     9781 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
     9841 gtgcggctgc acattgctgg gactactagg aagataaatg ccatcgagtt tcggcaaaag
     9901 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
     9961 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    10021 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    10081 cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    10141 ttttcaatct agcagtggat tttcattttt gcaaactcaa tttaacattt gacaaacatt
    10201 tggtttgcat acagattcga atgatggtca ttatatttta cgctttgata acgagcggaa
    10261 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    10321 gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg
    10381 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    10441 aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc
    10501 ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg gtcaggaaat
    10561 gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt ctgccgccga
    10621 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    10681 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt
    10741 cccggttgta gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg
    10801 ggatctgcaa ccgccctgcg cgcctggcac cgatgaccgc aatacacaaa gccaggttta
    10861 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg aactggcgca
    10921 gttgcagctg attccggcag atggcgcggt cgctgaggcc tcgccgggtc agtttgaaat
    10981 caacctttac catactgata acgtgctgga agcctgcgat gatgcgctgg cactaaaacg
    11041 cctagtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    11101 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    11161 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    11221 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    11281 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    11341 cgtcgccctg cgtattccgt gcggtgaccg tcataatcac cgcgtggaat atcacgtggc
    11401 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    11461 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    11521 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    11581 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    11641 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    11701 attccccttg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    11761 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    11821 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    11881 gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc
    11941 taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca
    12001 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    12061 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    12121 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    12181 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    12241 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    12301 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    12361 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    12421 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    12481 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    12541 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    12601 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    12661 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    12721 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    12781 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    12841 attgcgttgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    12901 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12961 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    13021 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    13081 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    13141 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    13201 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    13261 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    13321 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    13381 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    13441 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    13501 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    13561 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    13621 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    13681 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    13741 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    13801 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    13861 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    13921 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13981 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    14041 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    14101 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    14161 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    14221 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    14281 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    14341 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    14401 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    14461 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    14521 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    14581 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    14641 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    14701 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    14761 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    14821 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    14881 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14941 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    15001 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    15061 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    15121 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    15181 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    15241 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    15301 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    15361 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    15421 cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    15481 cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg
    15541 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattaccta ttctgccgct
    15601 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    15661 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    15721 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    15781 ttcgttggct tcagcttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    15841 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    15901 attaacggtc aaccaattgc cgaacagtta aaagagctct tcccggcgac gatagaactg
    15961 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    16021 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    16081 atcccggtgt tctggctggc gcttctgttg acactgtttt gttcactcac gctaggttgg
    16141 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    16201 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    16261 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    16321 ctgatgcgta tcagcactat tgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    16381 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    16441 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    16501 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    16561 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    16621 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    16681 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    16741 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcgggactgg ctgtactgtg
    16801 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    16861 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16921 cggacgtgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16981 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
    17041 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    17101 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    17161 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    17221 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    17281 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    17341 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    17401 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    17461 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    17521 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    17581 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    17641 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    17701 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    17761 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    17821 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    17881 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17941 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    18001 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    18061 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgaca
    18121 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    18181 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    18241 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    18301 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    18361 agcgcgatgc cgcataaaag tcgcctcaat actctacccg gcgctatccc attgctggaa
    18421 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    18481 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    18541 atggagaaaa agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    18601 ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
    18661 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    18721 actggcaaaa atgctggcgg gaatgataga acctaccagc ggcgagttat tgattgacga
    18781 tcatccgctg cattttggcg attattcctt ccgtagtcag cgtattcgca tgatttttca
    18841 ggatccttcg acctcgttga atccccgtca acgtatctca caaattctgg attttccact
    18901 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18961 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    19021 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    19081 tgaagccctc gcttcactgg atatgtcaat gcgttcgcag ttgattaatc tgatgctgga
    19141 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    19201 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    19261 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    19321 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    19381 ccagattatt cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    19441 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    19501 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    19561 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    19621 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    19681 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    19741 agattattct gcaggaactg aaccgccggt caccctcacc ctgaaagggc gagggggccg
    19801 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    19861 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19921 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttaggg
    19981 taaggggaaa agcgcccccc tgccgcagcc tgctccggtc ggacctggca actatagcta
    20041 ctcacagcca ggttgattat aataaccgtt tatctgttcg tactgtttac taaaacgacg
    20101 aatcgcctga ttttcaggca caacaagcat caacaataag gattaaagct atgggttttc
    20161 tttccggtaa gcgcattctg gtaaccggtg ttgccagcaa actgtccatc gcctacggta
    20221 tcgctcaggc gatgcaccgc gaaggagctg aactggcatt cacctaccag aacgacaaac
    20281 tgaaaggccg cgtagaagaa tttgccgctc aattgggttc tgacatcgtt ctgcagtgcg
    20341 atgttgcaga agatgccagc atcgacacca tgttcgctga actggggaaa gtttggccga
    20401 aatttgacgg tttcgtacac tctattggtt ttgcacctgg cgatcagctg gatggtgact
    20461 atgttaacgc cgttacccgt gaaggcttca aaattgccca cgacatcagc tcctacagct
    20521 tcgttgcaat ggcaaaagct tgccgctcca tgctgaatcc gggttctgcc ctgctgaccc
    20581 tttcctacct tggcgctgag cgcgctatcc cgaactacaa cgttatgggt ctggcaaaag
    20641 cgtctctgga agcgaacgtg cgctatatgg cgaacgcgat gggtccggaa ggtgtgcgtg
    20701 ttaacgccat ctctgctggt ccgatccgta ctctggcggc ctccggtatc aaagacttcc
    20761 gcaaaatgct ggctcattgc gaagccgtta ccccgattcg ccgtacagtt actattgaag
    20821 atgtgggtaa ctctgcggca ttcctgtgct ccgatctctc tgccggtatc tccggtgaag
    20881 tagtccacgt tgacggcggt ttcagcatcg ctgcaatgaa cgaactcgaa ctgaaataat
    20941 cgttctgttg gtaaagatgg gcggcgttct gccgcccgtt atctctgtta tacctttctg
    21001 atatttgtta tcgccgatcc gtctttctcc ccttcccgcc ttgcgtcagg ataacgattt
    21061 cctttacgac caaggagcgc ccatggaaca acgccacatc accggcaaaa gccactggta
    21121 tcatgaaacg caatccagta ctacggagta tgacgttctg cctctggtcc cggaagccgc
    21181 aaaggtcagc gatccctttc tgctcgacgt gatccttgat gaagagacgc tggccccctt
    21241 cctttcatgg ctggtccctg cgcgcgttct tgcagtggag ttgttccctg accagcttac
    21301 cgtgacccgt tcacaaacct tcaccgctta tgaacgcttg tcgacggccc tgacggttgc
    21361 tcaggtttgc ggcgtccagc ggttatgtaa ctactattcg gcgcgactta cgccgctccc
    21421 cggacctgat tcctccaggg aaagtaatca tcggttggca caaatcacgc aatatgcccg
    21481 ccaactggct agctcgcctt ctattatcga caaccgatcg cgccagcatc tgaatgacgt
    21541 cggtcttact gtctgggact gtgtgatcat taaccaaatc attggtttta ttggctttca
    21601 ggcgcggaca attgcgacat ttcaggctta tctcgggcat ccggtacgct ggttacccgg
    21661 gctggcgata caaaactacg ccgacgcgtc actgtttgct gatgaatcaa tacgctggcg
    21721 aagcagctat gaggtggaaa aactacctga agagcacaca aaaagttcaa ctgcagaact
    21781 ttgccaactg gccgaaacac tctctctcca ccctatttca ctttcccttc tcgaaaagtt
    21841 gttaaacagc acacgggtca atgcacagcc ggataatcag cttgcggcgt tgttatgcgc
    21901 gcgtataaat ggcagtcctg cttgtttttc cacctgtatg gattcatcaa atgaatataa
    21961 aaaaatcagc acccttctgc gcaagggcga aaatgaaatt aaccgatggg ctgaccgtca
    22021 ttctgttgag cgcgctaccg ttcaggcgat acaatggctg acccgagcac ccgatcgctt
    22081 tagcgccgcc cagttcagcc ctttactcga acacgaaaaa tcatcaacgc agattattaa
    22141 tctgctggta tggagcgggc tgtgtggctg gataaatcgc ttaaaaatcg cgttgggtga
    22201 gacatattaa ccttgccgcg tcagacagat tcgcgtaaaa ctgtcagccg ctctaatggc
    22261 caccaaaata gacaattatg tttcaggaca acccgctgct agcgcagctt aaacagcaac
    22321 tgcattccca gacgccacgc gctgaagggg tggtaaaagc cacagaaaaa ggctttggct
    22381 tcctggaagt cgacgcacaa aaaagttatt tcattccgcc gccgcagatg aaaaaagtca
    22441 tgcatggcga ccgaattatc gcggtgatcc acagtgaaaa agaacgtgaa tccgcagagc
    22501 cagaagaact ggttgaaccg ttcctgactc gtttcgtggg taaagttcag ggcaaaaatg
    22561 accgtctgac catcgttcct gatcatccac ttttaaaaga cgccattcct tgccgcgcag
    22621 cccgtggcct gaaccacgag tttaaagaag gcgactgggc ggttgccgaa atgcgccgtc
    22681 atccgctgaa aggcgatcgt tctttctatg ctgaactgac acaatacatc acttttggtg
    22741 acgaccactt tgtaccgtgg tgggttaccc ttgcgcgcca taatctggaa aaagaagcac
    22801 cagacggcgt cgctaccgaa atgctcgatg aaggtctggt tcgtgaagat ctgacctcgc
    22861 tggattttgt caccatcgac agtgccagca ctgaagatat ggatgacgcc cttttcgcta
    22921 aggcgctgcc ggatgacaaa cttcagctga ttgtggcgat tgccgatcca accgcgtgga
    22981 ttgctgaagg cagcaagctg gacaaagccg cgaaaattcg cgcattcacc aactatctgc
    23041 ctggcttcaa catccctatg ctgcctcgcg agctttctga cgatctctgc tcactgcgcg
    23101 ccaatgaagt ccgcccggta ctggcatgcc gcatgacgct ctccgctgat ggcaccactg
    23161 aagataatat cgaattcttt gccgccacca tcgaatccaa agcgaagctg gtgtatgacc
    23221 aggtttctga ctggctggaa aataccggtg actggaagcc tgaaagtgaa gcaattgccg
    23281 aacaagtccg tttgctagcg caaatttgcc aacgccgcgg cgagtggcgt cataaccacg
    23341 cactggtgtt taaagatcgc ccggattacc gctttattct cggtgaaaaa ggtgaagtgc
    23401 tggatatcgt cgccgagcct cgtcgcattg ccaaccgtat cgtcgaagaa gcgatgattg
    23461 ccgctaacat ttgtgcggcc cgcgtactgc gcgataagct cggttttggc atctataacg
    23521 tgcatatggg ctttgatccg gcgaatgccg acgcgctggc agcgttgctg aaaacgcacg
    23581 gtctgcatgt cgatgccgaa gaagtgctca cgctggacgg tttctgcaaa ctgcgtcgtg
    23641 aactggatgc gcaaccaact ggtttcctcg acagccgcat tcgtcgcttc cagtcatttg
    23701 ctgaaattag ctctgaaccc ggtcctcact ttggcctcgg tctggaagca tacgccacct
    23761 ggacttcgcc gatccgtaaa tatggcgaca tgatcaacca ccgtctgctg aaagcggtta
    23821 tcaaaggcga aactgcgacg cgtccgcagg atgaaatcac tgtccaaatg gccgaacgtc
    23881 gccgtctcaa ccggatggca gaacgtgatg ttggcgactg gttatacgca cgcttcctga
    23941 aagataaagc cgggaccggc acccgtttcg cggcggaaat tgtcgatatc agccgtggcg
    24001 gcatgcgtgt gcgtttggtt gataacggcg ctatcgcctt tattccggca cctttcttac
    24061 acgctgtgcg cgatgaactg gtttgcagcc aggaaaacgg cactgtacaa attaaaggtg
    24121 aaacggttta caaagtaact gacgttattg acgtcaccat tgccgaagtc cgcatggaaa
    24181 cccgcagcat tattgcgcgc ccggtcgcgt aatctccttt cacggcccat tcctcatgga
    24241 tgggccgttt atttccccgc tctaccttcg ttatatccag gcagattttt taactgactt
    24301 tcgtttgaaa actggcgttt ttccaaaact ggattacaaa taaatatata aaaaacaatg
    24361 cgtttagccg ccgttaattt catgtaagtc atgttgttcc ttttatttaa ccgcggactc
    24421 cgctgttaac cggaggatat gcatcatgaa aaccgttagg gagtccacaa cgttgtacaa
    24481 ctttctcgga tcgcacaatc catactggcg gttgacggaa agcagcgatg ctttgcgctt
    24541 ttctaccacc aaaaccacag aacctgatcg tacattgcag ttatctgccg aacaggctgc
    24601 tcgcatcagg gaaatgacgg tcatcacctc cagcctgatg atgagtctga ccgtcgatga
    24661 aagcgatctt tctgtgcatc tggtaggacg aaaaatcaat aaacgggaat gggctggcaa
    24721 cgcgtctgca tggcatgaca caccggcggt tgctcgtgat ttatcacacg ggctttcctt
    24781 tgctgagcag gtagtttctg aagcacattc cgcaatagtg attctcgaca gccgggggaa
    24841 tatccaacgc ttcaatcggt tatgtgaaga ttacacaggg ttgaaagaac acgacgtcat
    24901 tgggtaaagc gtgtttaaac tgtttatgag ccgtcgtgaa gctgcggcat ccaggcgcaa
    24961 taaccgtgta ttttttcgaa gcggcaatgc atatgaagtc gaactgtgga taccaacacg
    25021 taaaggccag cggctgtttc tgtttcgcaa taaatttgtc cacagcggca gtggcaaaaa
    25081 cgagattttt ttaatctgtt ccggtaccga cattaccgaa gagcgccgca ctcaggagcg
    25141 actgcgtatt ctggcaaata ccgacagtat caccggactg ccgaatcgta acgcaatgca
    25201 ggagttaatc gatcacgcta ttaatcaggc agataacaat aaagttgggg ttgtgtatct
    25261 tgatttggat aattttaaaa aggtcaacga cgcctatggg catttgtttg gtgaccagtt
    25321 attacgcgac gtgtcattgg ctattttaag ctgtctcgaa catgaccagg tgttggcgcg
    25381 tccaggtggc gatgagtttc tggtactggc atccaatacc tcacaaagcg cgctggaagc
    25441 aatggcatca cgaattttga cccgcttacg cctccccttt cgtattggtt taattgaagt
    25501 ttataccagc tgttcagtag gtatttcgct ctctcccgaa catggttcgg acagcgcgac
    25561 tattattcgt cacgccgaca cagcaatgta cacagcgaag gaaggcggac gaggacaatt
    25621 ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa tatctctggc tggataccaa
    25681 cttgcgtaaa gcactggaaa acgatcagtt ggttatcgaa aatcacctag cgtggcgaag
    25741 tgcgcagtct ggaagcacta gtacgttggc agtcacctga acgtgggttg attccaccgt
    25801 tggacttcat ttcctacgcc gaagagtcag ggctaattgt gcctttaggc cgttgggtga
    25861 ttctcgatgt cgtacgccag gtggcaaagt ggcgggataa aggcataaac ctgcgagtgg
    25921 cggtaaatat ttctgcacgt cagctcgccg atcaaaccat tttcaccgcc ctgaaacagg
    25981 ttctccagga actcaatttt gaatactgcc ctatagatgt tgaactgaca gagagttgtc
    26041 tgattgagaa tgatgaactg gcactgtctg ttattcaaca atttagccaa ctaggtgcgc
    26101 aagtgcatct ggatgatttt ggtaccggct actcttcact ttcgcaactg gcgcgctttc
    26161 cgatcgatgc catcaaactt gaccaggttt ttgttcgaga tattcacaaa caacctgtct
    26221 cgcagtcact ggtccgggcg atcgtcgctg tggcccaggc attgaatctt caggtgatcg
    26281 ccgaaggtgt agagagtgca aaggaagatg cttttttaac caagaacggg atcaatgagc
    26341 ggcaaggatt tttgtttgcc aaaccgatgc ccgccgtcgc cttcgaacgc tggtataaac
    26401 gctatctgaa gcgcgcataa gtagatgaat atatatactt agcgctatat cccgtcatta
    26461 tatatcgtat tcctggatga taattagccg ttgctgttaa acacttctct tttagcggca
    26521 atttttgcat tattcatttt cccccttttg ctataaggat gtccttgatg tctgaatttg
    26581 atgcccagcg cgttgccgaa cgtattgata ttgtgttaga tatccttgtg gcaggcgatt
    26641 accactctgc gatccataat ctcgagatac ttaaagcaga actcttgcgc caggttgctg
    26701 aatctacgcc agacatcccg aagacaccgt gggagattta acaatcgtgc cttcaggttt
    26761 tcctatttga ggatgcccgg cgcgtcagta tttttttatt tagtattata acgttataag
    26821 aattacagcg atgaattccc gacaacaaac tattctacag atggtcattg accagggtca
    26881 ggttagcgta accgatctgg caaaagccac tggagtttct gaagttacca ttcgccagga
    26941 tctcaacacc ctcgaaaaac tgagttacct ccgccgtgca catggctttg cagtttcgct
    27001 tgatagtgat gacgtcgaaa cccgtatgat gagcaactat acgctgaagc gtgaactcgc
    27061 cgagtttgcc gcgtcactgg ttcaaccggg cgaaaccatc tttatcgaaa atggcagcag
    27121 caatgccctg ctcgcccgga ctctggggga gcagaagaaa aatgtcacta tcatcacggt
    27181 cagcagctac atcgcgcatt tgctgaaaga cgcgccttgt gaagtcattt tgctcggtgg
    27241 cgtgtaccag aaaaaaagcg aaagtatggt tggccctttg acacgccagt gcatccagca
    27301 ggtacatttc agcaaagcat ttattggtat tgatggctgg caacctgaaa ctggattcac
    27361 cggtcgcgat atgatgcgca ccgatgtggt caatgccgtg ctggaaaaag agtgcgaagc
    27421 gatagtcctg actgacagct cgaaatttgg tgctgtacat tcatactcca tcggtcccgt
    27481 tgagcgattc aatcgcgtga ttaccgattc gaaaatacgc gccagcgatc tgatgcatct
    27541 tgagcacagt aaactcaccg ttcacgtcgt tgacatttaa ccatttccct tctctacgga
    27601 tgatttgcag tttggcaaat catccgctct aagatgattc ctggttgata attaagacta
    27661 tttacctgtt attaacactc tcaagatata aaattattat cagcgatata acaggaagta
    27721 attatcacct gcgtgatata accctgcgcg cgagcagatt tcacggaata atttcaccag
    27781 gcttattctt agctattata gttatagaga gcttacttcc gtgaatcata aattcaggag
    27841 agagtattat gtttgtaacg agcaaaaaaa tgaccgcggc tgttctggca attactttgg
    27901 caatgtctct gagtgcctgt tctaactggt ctaaacggga ccgcaacacc gcaatcggcg
    27961 cgggtgcagg ggcattaggc ggtgcagtac tgaccgatgg cagtacgttg ggtacattag
    28021 gtggtgcagc tgtcggtggt gttattggtc accaggtcgg taaataatcg taaataaatg
    28081 tcgattatcg actcgcaatt ataatacgct tgattctgtt cagtacgaaa taactgaata
    28141 atacacacag taaaatattc gaaagccacg gatatatccg tggcttttta tgttaaccgc
    28201 ctgcgagttt taccttcatc cctttcgctt caagtagtga ttttaataaa tcacgcttat
    28261 cgccctggat ttcaataact ccatctttaa ctgctccgcc gcagccgcat tttttcttca
    28321 gttccgctgc aagttttgtc agttcggcat catcgagatc gataccggta atcaggcaaa
    28381 cgcccttacc tttacgtcca ctggtctgac gctgaatacg caccgctcca tcgcctttag
    28441 ggcgtactgg ggccgctttg ggctcatcaa tacgcccggt ttccgttgag tagaccagac
    28501 ggctgttgga atcactcatc atgcactccg ctgtaaagag gcgttgatcg ctttcagtgt
    28561 ctgcgctgga tctaccgatt gcgttaccgg gcgaccaatc accatataat caacaccagc
    28621 cgccaacgcc tgttctggcg tcataatgcg gcgctggtca ccagcttcac tcccctgcgg
    28681 acgaatgccc ggtgtaacca gtttgaactc ctgaccgaat acctgtttaa agcgcacagc
    28741 ttcctgagca gaacacacca caccatcaag gtcacatttt tgcgtcagtg ctgccagacg
    28801 ttctgcataa tctgcaggtg acagtgtcac gccaagatcg gccaggtcgc tggcttccat
    28861 gctggtcaac actgtcacag caatcaaaag cggtgcatct ttgccaaacg gaaccagtgc
    28921 ctcacgcgct gcggtcatca tacgcgcccc accggaggca tgaacattca ccatccacac
    28981 gcctaagtca gctgcagcag cgacagcgtg cgctgcagtg ttggggatat cgtggaattt
    29041 caggtcaaga aagatatcaa aaccacgctg ttgaagttcg cgcacaaact gtggcccaaa
    29101 caatgtaaac atctctttac cgaccttcag acgacaatcg cgtgggtcga tcttgtcgac
    29161 aaaggacagc gcgtcatcac aattatgata atcaagggca acaaccacag gagaattcgt
    29221 aacggcgcgg gaagaagatg aagcagttaa cgtcatgacc agaccttctt gatgatgggc
    29281 accgggagtg gcgcgaaaca ggtaaacggc gagcattcta cccgtgaaaa taaaaaatta
    29341 acagttgcga tttttcccct agcaggcttt ttctgctccg tcgttgccgg agcacaatgg
    29401 gacatcaata actagtatgt tgtaactaaa gtcggatttt tttaaaaatt acaggccatc
    29461 aagaccgcga atcggtttaa tagttgacca ggcacgacaa gacggacaat gccagtagag
    29521 ggtgtatgcg gtaaaaccac atttctggca gctataacga ggcttactac gcaccttctc
    29581 gccaaccatg tcacgcagca ccatcagact ctctttggcg cgcccttctt ccgcttcatt
    29641 taagtggtaa tccattagct tatggaatac acgcatggtc ggatgacgct gaagctggcg
    29701 tgtaatatag acctgtgcag cctcactacc gtcgcgcgct tcgatgatat cagcaagcat
    29761 caattcagca tcggcaccgg tgttctcttc caccgcgcgt tgcaggaatt ctgcccattc
    29821 ggcagtttta cccaactgct gatagcaggt ttgcaacatt tccagcgttt cgctgaccag
    29881 ttctctgtcc tgggatatga cgcgttgcaa actttcgacg gctttggcgt attctccttt
    29941 cgccataaac acgcgtccca tcatgatgga tacgcgggcg ctgtttttat ctgccgccgc
    30001 cccttttttt agcaaggtca tggcacgatc gagatcgtcg ctggccatat gctgcagggc
    30061 taactcacag tagaaatggg caatttcgac gcgctgttta tctttaccca gcttcaccag
    30121 gcgttcggca acatcaattg ctttctgcca ctcactggta gcctggtaga tttgtagcaa
    30181 ctgttgcagc gcgccaatgc ggaagtcagt ttcatcggtc agctgattga acatgtcttc
    30241 cgcgcggtcg tataacccgg cggccatgta atcacgcccc agttgttgaa tcgccaacag
    30301 acgctgttca taggtcagcg aggcgctttc cattagggtc tgatggatgc gaatagcgcg
    30361 atcaacttcg ccacgcgaac ggaacaggtt tccgagcgta aggtgggctt caacggtgcc
    30421 tgtatcctct ttaagcatat cgagaaacag gtctaccgct ttatcctgtt gattactaag
    30481 caggaagtta acccccgcta cgtaatcacg cgacaagcgg ttggcttcat cttgcttgtt
    30541 ttgttgcgca cttctgcggc ccatatacca gccataggca gcggctacag gcaaaagcag
    30601 aaacaacaac tccagcatag aaagttattc cttcgccgct gacgagtgcg gcactacagc
    30661 cacgtcagtc gcgggtgaaa gctggttttc cagtcgcttt attttacgtt cagcgcgcgc
    30721 cagggaaaca cgaactcgca gccagaacag gccacaaatc aaccaaccga tagcaaaccc
    30781 cgcagcaaac aataccgcca gcaatgtgga aatacggtac tccccttgcg ctaacagata
    30841 attaaacgtc acctgttgat cgttctgcgc acccaacgtg accgaaatca cgaagatcgc
    30901 taacaccagt aagaaaatga gtaaatattt cacattactt cccgttatgt ggttagagcg
    30961 aataaattat ggtcaactta ccgcaatagc cctttaaagt accattttcg cgccgtctgc
    31021 gaaatggcat ccacgcgcat tgttgatgat ttaaggccta aattcggaaa atcaaccagc
    31081 gttaactttc ttgttctcgt tgtgcaattt cgcgattttc ttccgcaggt ggtgttaatg
    31141 gcccacaaat tcgttgcgca agccacgttg ccaccgccac cagcgcccac gaaatcaacg
    31201 tagctaccac cagatcgcgt ggccaatgca tcccgagcag caggcggctt cccatgactc
    31261 ccgttgccca gaccaacaag atagcaatgg ttaacgttcg ccgacgcggc cacagcaaac
    31321 caacggccag cagtgcccaa ctggcagcaa acatcgtgtg accggaagga aaggcaaacc
    31381 ccgtctcttt ctgccagtgt gaacgcaaat attgtggaat atttttctct tcagccaact
    31441 gttcttttac tagatttccg cgttctgctc gctttaaagt gtagaactca tcaaccggaa
    31501 tatgatgtgt tttttccaac cagataacaa aaggtcgtgg ttcctgtact ttgtctttga
    31561 tccaggattt aacgccttgt ccaacaagga ttgccgccgc cagaatggca aataatacaa
    31621 aggcagcctt aatgcgaaaa cgcagacacc agagaaacca gccgaacaaa atcaaatgtg
    31681 taatgacgcc ccagggctgg gtgacagttt cagtaaccca aaaagccgct tttagtagcc
    31741 aactttgttc tccaggttgc caacgccagc cagaaatcca tacggctact ggcatgacaa
    31801 gcaatagtgc agctcccact gcggtacgtc gggcaatcga acgcatggcc tctccttttt
    31861 gataagtccc acaatcataa ctgaaaatgc cagttccaag aaaaattgac agatttgtgc
    31921 cattccgtga acgatcgacg cgtcgtgatt aggtgaaccc cttctcgtta tggcaaaata
    31981 agccaataca gaaccagcat tatctggaga atttcatgca gcttaaacgt gtggcagaag
    32041 ccaaactgcc aaccccatgg ggcgatttcc tgatggtggg atttgaagaa ctggcaaccg
    32101 gacacgatca tgtcgcgcta gtctatggcg atatttccgg gcataccccg gtacttgcgc
    32161 gcgtccattc cgaatgtctg accggtgacg ccctgttcag cttgcgctgc gattgtggct
    32221 tccagctcga agcggcattg acgcaaattg ccgaggaagg ccgtggtatt ttgctgtatc
    32281 accgtcagga aggtcgtaac attggtctgc tgaataaaat ccgcgcttac gcactgcagg
    32341 atcaaggtta cgataccgta gaggctaacc accagttagg cttcgccgct gatgagcgcg
    32401 acttcactct ttgcgctgat atgttcaaac tccttggcgt caatgaagtc cgcttgttaa
    32461 ccaataaccc gaaaaaagtc gaaattctga ccgaagcagg gattaatatt gttgaacgcg
    32521 taccattgat tgtaggtcgt aaccccaata acgaacatta tctcgatacc aaagccgaga
    32581 aaatgggcca tttgctgaac aaataaccct cttgcatcgt gtaattcatt tgcttgccgg
    32641 aagcaaaata accggcaagc aaatacttgt tacttcaaca tattacgaat gacataatgc
    32701 aaaatgccgt cgttctggta gtaggtcaac tccgtcgcgg tgtcgatacg acaacggcag
    32761 ggtacgactt cctggctacc atccgcgcgc gtaagcgtca ccggaaccgt cgcgccaggt
    32821 tgtagatttt gcagatcgcc aatatcaatc ttctcttccc cggttagccc taacgtttta
    32881 cgcgtcacgc cttgcggaaa ttccagcggc aggatgccca tgccaattaa attcgaacgg
    32941 tgaattcgtt caaacgattc ggcaatcacc acacgaattc caagcagacg aggtcctttt
    33001 gccgcccagt cacggcttga gcctgatcca tactctttcc cggcaatcac cgccagcggc
    33061 gtttgctcct gcttatagcg catcgcagca tcataaatag agactacgtc gctgtcaggt
    33121 aaatgccgcg tcatcccccc ttcaacgcca ggcaccattt cattacggat gcgaatattg
    33181 gcgaaggtgc cgcgcatcat cacttcatgg ttaccacgcc gcgaaccgta ggagttaaag
    33241 tcttttcgct caacaccccg accttgtaga tatcgacccg ctgggctgtc gggcttaata
    33301 ctgcccgccg gagagatatg gtcagtggtg actgaatccc ccagcattgc gaggatccgc
    33361 gcaccgtgaa tatcttccac tggtgctggt gttgcctgca tttcatcaaa gaaaggcgat
    33421 aagcgaatat aggttgagtc ctcctgccaa ccgtaggtat cggatcgtgt gacgttaatt
    33481 tccttccact ctgctgtgcc ttcaaaaact tctgcgtact ctttgcggaa catttctgtg
    33541 gagacttgtt ctaccgcacg ggcaatttct tgtgccgatg gccagatatc tttcagataa
    33601 accggctcgc ctttgcgatc atggccgata ggctcagaag ccaggttgat attcatattt
    33661 cccgccagcg cataggcaac caccagcggt ggcgaggcca gccagttagt tttaaccagc
    33721 ggatggatac ggccttcaaa gttacggttg ccggacagca ccgcaccgac ggttaaatcg
    33781 ccttttttga ttgccgtttc gataggatcg ggcagcggcc cagagttacc aatacaggtg
    33841 gtacaaccgt atcccacaag gttaaacccc agttcgtcga gatacggtgt cagtttcgct
    33901 tttgccagat aatcagaaac gactttcgaa cccggtgcca acgacgcttt gacccacggt
    33961 tgccgcttga ggccaagagt tacggctttt ttcgccagca agcctgcggc catcagcaca
    34021 cttgggttag aggtgttggt gcacgaggtt atcgcagcaa tgaccacagc gccatcaggt
    34081 aactgatact gatgtccgtt cataacataa tcgactggca ggcgatcttt atgcgtggca
    34141 ttcacttcca gttcgttact ggcggcaaat gcttttggta catcgggcag tgcaacgcga
    34201 tcctgtgggc gttttggccc tgccaggctc gcttcaacgt cattcatatc cagttctaac
    34261 atactggtaa aaatcggttc atcacccggg ttacgccaca tgccctgcgc tttggcatat
    34321 ttttcgacca actcgacctg atcttcgctg cgcccgctta aacgcatgta atcgagggtt
    34381 actgcatcga ttgggaagaa gccacaggtg gcaccatatt ctggcgacat attggcaatg
    34441 gtggcgcgat ccgccaacgg tagtgaatcc agaccatcac cataaaattc gacgaatttc
    34501 cccaccacgc catgtttgcg cagcatttgg gtaacagtga gaaccaggtc tgtggcggta
    34561 ataccttcac gtaattttcc ggtaagttta aagcccacta cgtccgggat aagcatggaa
    34621 accggctggc ctaacatggc ggcttctgct tcgatcccac ccacgcccca ccccagcacg
    34681 ccaaggccgt tgatcatagt ggtgtgcgag tcagtaccaa cgagtgtatc cggataagca
    34741 atccattcac cgtcctgcaa ttcgctccac actgctttgc cgagatattc gaggttaacc
    34801 tgatggcaaa tgcctgtgcc tggcggcacg acgctaaacc gactgaacgc ttgctttccc
    34861 catttcagga acacataacg ttcgtggttg cgctccattt ccaggcgtac gttttcttca
    34921 aatgcctcat catcaccaaa acgatcgacg gtcaccgagt ggtcaatgac caggtcgacc
    34981 ggtgagagcg ggttaacctt tgcagtatcg ccaccgaggc gtttaaccgc ttcgcgcatt
    35041 gccgccagat caacaacggc aggtacgccg gtaaagtcct gcatcagcac ccttgccggg
    35101 cggtaggcaa tttcacggtc agcatgggca tttttcagcc atcccgccag tgcgtggata
    35161 tcctcttcgg taactgagtt accatcctgc cagcgcagca ggttttcgag caaaactttg
    35221 agtgacttgg gtagacgggt gatatcgccc agtgatttag cagcaagcgg caggctgtag
    35281 tagtggtaag ttttatcttt ggcctgcaac gtgtccttac tggcttctcg tagggttgac
    35341 gacatagctc ctccttaatg acagggttgc gaagccctga ttctcttcag ggtgaattta
    35401 aagataacac aaacatcgcg taacgatttg ataacaaccc aaattgataa aagaggagaa
    35461 accttgtggc aaagcaaatg acaaccccgc cgcagcgggg tcaaggatca gtgagtaatg
    35521 atccagagga gttcggccca gaagagcatc gacagagaaa acgcgcccat ccaggaccaa
    35581 tatttcatat ttgtgttgat cataggctac acccttaacg gttattattt tgtgttgaga
    35641 taactcaagc tgttattgct gcggtgtatt aaaagccctt taattcatta aaaatgggta
    35701 ataagaatga tgataattat tgtttcggct gatcatcttc agcaaacagt tctataaagg
    35761 cttgctgctg cgatgttagt tgccagtcgt ctggaataac gacggaacat tcccgtttcg
    35821 tttctggttc attgtcacta tgctggcata atggtgacga ctccagttgc tcctgaccga
    35881 ccatagttat ccccacactt tccaaatcag cagtgcgaca accacccaga aaagtgcaga
    35941 accaagaaaa accgccagcc aggctttacg ttttaattca ggatcacggc ggggttcctg
    36001 atttccagaa ggcattgcta acctcatata atcgccatca cttatcagca agacgttgaa
    36061 tgattggtgt aattaatcat acgatgagac aaatcctaaa gaaactttag tcaaaaatcc
    36121 agcgaattta cgaatcgtct ccagtgaata attcaatctt ttgacctgaa atagatattt
    36181 gaaaactgga atttgcgagg cgggtaatta gacactactt tacaggtaat ttaccctata
    36241 aatatgaggg ataaaaatat tgtgcacggt atagaaataa gtaacaaaag agaatatatt
    36301 ccggcacctt cgctacataa aaggtgccga aaataacgca agaaattatt tttccggcag
    36361 ttttatatct ttaaacatga cctcaatttc ttcattggag cgcaatgcga cagccgcatc
    36421 aacgacatca cgcgttaaat gcggtgcaaa acgctgaatg aaatcataca tataactgcg
    36481 caaaaaagta ctacggcgaa aaccaatttt ggttgtactg tggctgaaga tatcgtgagc
    36541 atcaacgcgc acaaggtcgg gatcggcgac cggatccacc gccatgctgg caatgacccc
    36601 tacccccagc cctaaccgga cgtaagtttt aatgacgtca gcatccgttg ccgtgaaaac
    36661 gatacgcggc gttaaccctg cgcggttaaa ggcagtatcc agttctgaac gtccggtaaa
    36721 gccgaaggta tatgtcacca acggatattg cgccagttct tcaatggtaa tggctttttt
    36781 gcctgccagc gggtgatccg gagtgactac aatagcccga ttccagtggt agcacggtaa
    36841 catcactaaa tcttcataca gatgcagcgc ttctgtggcg atagcgaaat cggcattgcc
    36901 tttagagacg gcatcagcaa tttgtgtcgg cgggccctgg tgcatatgca aagaaacgcg
    36961 gggataacgc tcaataaagc ctttgataac gtttggtaac gcgtagcgtg cctgggtatg
    37021 cgtggtggcg atatacagcg agcctttatc cggccaggtg tgctcaccgg caaccgattt
    37081 tatggcatcg actttcgaca ggacttcgcg agcgatacga attatttctt gtcctgctgg
    37141 cgttacctgc gtcaggtgct tgccgcttcg ggaaaaaatt tgaatgccta gctcgtcttc
    37201 cagcattctg acttgtttac tgatcccggg ttgtgatgtg taaagtcctt ccgctgttga
    37261 tgagacattc aggttatgat tgaccacctc aacaatatag cgaagttgtt gtaatttcat
    37321 gttaaaccat ccacttagac gccatcagaa catcgttttt tcgtcacacc tggcgggtga
    37381 ctaatacgat ttaataataa aaaaggtgct aactataacc actatatcat ttatagcctt
    37441 agtgtatagg tgtgaagaaa aaataatatg caataaaaaa gggccgcttt cgcgaccctt
    37501 tgtttataaa aacctgacag aattaaaggt tatttttttc cttcaaccca tttgccatca
    37561 acataaaatg ctgaccagcc agtcgccttt ccgtcttttt ccgaagagac atattgctgt
    37621 ttggttttac ggctaaagcg aaccatggtc ttattacctt ccggatcctg ctgtggcgca
    37681 tcggccagat aacgcagttt ttccggcaga cggtcgcgga agcgataaag ctcttccacc
    37741 agtggcgcac gcgtttcacg cgatttcggg aaagtgttgg cagccaggaa cacaccggca
    37801 gcaccgtcac gcagcacgaa ataagcatct gatttttcgc acggcagctc aggtaatggc
    37861 accggatctt ctttcggtgg tgccacttcg ccgttacgta aaatcttacg tgtgttttta
    37921 cactcttcgt tggtgcaggc catgtattta ccgaaacgcc ccattttcag gtgcatttca
    37981 gagccacatt tttcacactc aacgatcggg ccgtcataac ctttaatgcg gaattcgccc
    38041 tcttcgatct cgtaaccgtc gcaggttggg ttattaccac agacatgcaa cttacgtttc
    38101 ggatcgatga gatagctgtc catcgccgtg ccgcatttcg ggcaacgacg ttttgcgcgc
    38161 agcgcgttgg tttcagcatc ttcgccttcc agcacgttca gcacttcgtt ttccggcacc
    38221 aggttaatgg tggttttgca acgctctttc ggcggcagcg catagccaga acagccaagg
    38281 aataccccgg tactcgctgt gcgaatcccc attttgcgac cacaagtcgg gcagtcaatg
    38341 ctggtcagaa ccatctggtt cgggcgcata ccaccctctt ccggatcttt ttcagcttta
    38401 tctaactgct gggtgaaatc cgagaagaag tgatccagta cagctttcca ctctgcttcg
    38461 tgatttgcca cctggtcgag gctgttttcc atctgcgcgg taaagtcgta gttcattaac
    38521 tcgcggaaat tttcttcaag gcgatcggtg acgatttcgc ccattttttc cgcatagaaa
    38581 cgacgatttt ctactcgcac atagccacga tcctgaatgg tcgaaatgat cgacgcatag
    38641 gtagacggac gaccgatacc acgtttttcc agttctttaa ccagcgatgc ttcactgaaa
    38701 cgggctggcg gcttggtaaa gtgctgggct ggtgttagtt caacgagcgt cagagcatcg
    38761 cctttatcga ctgccggtaa gatacgatct tcatcgcctt tacgcagtgc aggcatcact
    38821 tttgtccagc catcaaagcg caaaatacga ccgcgtgctt tcaggcggaa atcgcccgca
    38881 ccaacggtca gcgtcgtgga gtcatatttc gctggggtca tctggcaggc aacgaactga
    38941 cgccagatta actggtacag tttctgcgca tctgcttcca tatccttcag cgattccgcc
    39001 atcacattga cgtcagaagg acgaatcgct tcgtgcgctt cctgtgagtt ttctttgctg
    39061 gcgtactgat tcggactttc cggcagatat ttcttaccaa aattatcgct gatataaccg
    39121 cgaaccatat ttaccgcgtc ctgactcagg ttagtggagt cggtacgcat gtaagtgata
    39181 tagcctgctt catacaaacg ctgcgccatc atcatggttt ttttcacgcc aaaaccaaga
    39241 cgggtgctgg cagcttgttg cagcgtagag gtaataaaag gagcgccagg tttactggtt
    39301 gtcggtttgt cttcacgttc cagcacgctg tagcgcgctt tttccagcag acttaccgca
    39361 gcctgagttt gttctttgtt gaccggacgg aacggtttgt cattctgatg agtcacctgc
    39421 aacgccaacg cttcaccaga tggcgtagtc gtgctggcat cgacttccca gaactcttcc
    39481 ggtacgaacg ctttaatttc acgctcacgc tctaccacca ggcggaccgc caccgactgc
    39541 acacgaccgg cagacagacc acgagcgatc tttttccaca gcagcggcga aaccatatac
    39601 cccaccacgc ggtccataaa gcgacgcgcc tgctgggcat taacacgatc aatattcagc
    39661 tcacccggtt tgttaaatgc ctggcggatc gcgtttttag taatttcgtt aaacaccact
    39721 cggctatagc gcgcatcatc accaccaatc acttcccgca ggtgccatgc aatggcttcc
    39781 ccttcgcggt caaggtcggt tgcgagatag atgtggtcgg ctttttcagc cagttgtttc
    39841 agttcagaga cgaccttctc tttaccaggc aacacttcat agtgcgcctc ccaattgtgc
    39901 cacgggtcaa cccccatacg gttgacgaga gcgccacgtt catccttttt aggcttttta
    39961 gccgtcttgg tggaggtaga gtcggcactc tttttggcag ctgagccact ggtcggcaaa
    40021 tcgcggatgt gaccgacgct ggatttcacc acgtagtcac aacccagata cttgttgatc
    40081 gttttggctt ttgccgggga ctcaacgatg acaagagctt tacccatatt caccttacct
    40141 aatttgattc ttccaggaat gcgtcgcacg ttgataaacc ttccactggc aacgagttac
    40201 cgatatggac tcgctctctg cggatatcaa ccccttgtcg tgactctgtt cacgaaaatt
    40261 caggtgactg agttaacagg tcttggccta caggcgctat agcacgacgg aattttggtc
    40321 gaatgtcaag caaatctatt gccagattga cgaaagcgtc acactgtacc tgataaaatc
    40381 cgatacgcaa ctttattagc atgcaaaatc caaagttgcc agtaaggcgc tcttgccacc
    40441 agcgtggtat atttgtctgc aagccatgcc tggcgtagac taatgtcctt gtttaaggag
    40501 taaataatga acaaagagac acaacccatc gaccgcgaaa cgctgttgaa agaggccaac
    40561 aaaatcattc gtgagcatga ggatacgctc gcagggattg aagccaccgg cgtcacgcag
    40621 cgtaatggcg tgctggtatt taccggcgac tactttttag atgaacaagg attgccaaca
    40681 gccaaaagca ccgcggtgtt taatatgttc aaacacctgg cccatgtgct ttcagaaaaa
    40741 tatcatctgg ttgattaaag caaaacgcga ggtcttagcc tcgcgtttgt cttttacatc
    40801 aatggctttt gaccccgctg ccaccagcgt agcaacaatc gatcggcgct ctctgccgcg
    40861 ctgccggtga atcggtcaat gagtcgttta cgctgcatat agcgtacatt aaccacttca
    40921 cggccttcca tcaggctaag aataacttca tcactggtgt tgatttcatc aaccaggcct
    40981 ttctctaccg cctgttgtcc gtaccagtgt tcacccgttg ccacctgttc aatatccaga
    41041 gacggacgca tacgcttcac aaaatcttta aataactgat gcgtttcgtt cagctcttcg
    41101 cggaatttct cccgcccttc ttcggtattt tcacccagca aggtcagcgt gcgcttatac
    41161 tgcccggcgg tgtgcagttc gatatcaata tctttgcttt tcaggaagcg gttaaagttg
    41221 ggcatttgcg ccaccacccc aatggaaccc acaatagcaa acggtgcgga aacaattttg
    41281 tccgccacac aggccatcat gtaaccgccg ctggcagcga ctttgtctac cgtaacagtt
    41341 aaaggaatgt ttttatcacg cagacgctgc agctgcgaag ccgccaaccc gtaaccatgc
    41401 accatgccac cagggctttc cagacggagc acaacctgat cctgcggttt gaatgctgcg
    41461 agtaccgccg ttatctcttc acgtagcgag ttcacttcat gggcgtccat gctgccttta
    41521 aaatccagca cccagacgcg aggtttactg tcagttgcca cctcgcccag tttggctttc
    41581 gcttttgctg ctttcgcttc ttgcttgtgc tttttcttct gcgctttgtg ccactgtttt
    41641 tgctgatgtg agtccatcag cgccgcggcc agttcttctt tcatctcctt atactgttcg
    41701 ctgagattgt tgacccgtaa ctcgccacgc tggcgtttat tacgttgagc aacattgaca
    41761 ataatggcgg caatcgccgc aatcgctagc acaacggtaa cgattttagc caaaaacaaa
    41821 ccatattcag acaacaattc cacgagtccc caccttggtt aaacaacgct ttggctcaca
    41881 gtttacaaca ggcatgttaa gccgtctcgc agagatgtta ttgcagcgaa agtatgacaa
    41941 agtatccatt ttgcggtgtt atcgccccag gctgattgaa atgcccttct gtttcaggca
    42001 taaagcccca aagtcataaa gtacactggc agcgcgacag acaacgtcgt tgaggagtag
    42061 ccatgcatta ccagccaaaa caagatttac tcaatgatcg cattatcctg gtgacgggag
    42121 ccagcgatgg gattggtcgt gaagccgcga tgacgtatgc acgctatggt gcgacagtga
    42181 ttctgttggg ccgtaatgaa gaaaaattac gtcaggtagc cagccacata aacgaagaaa
    42241 ctgggcgtca gccacagtgg tttattctcg atttgctgac ctgcacgtcc gaaaattgcc
    42301 aacaactggc acagcgcatt gtcgttaatt atccgcgtct ggatggtgtt ttgcataatg
    42361 ccggattgct cggcgatgtt tgcccaatga gcgaacaaaa tccgcaggtc tggcaggacg
    42421 tcatgcaggt caacgttaat gccaccttta tgctcaccca ggcactgctt cctttattac
    42481 tcaaatcgga cgccggttca ctggtcttta cttcatcaag cgttggacgt cagggacgag
    42541 ccaactgggg tgcatatgca gcgtcgaaat ttgccaccga agggatgatg caggtactgg
    42601 ccgatgaata tcagcagcgc ctgcgcgtca actgcattaa cccaggcggt acgcgcaccg
    42661 caatgcgcgc aagcgccttc ccgaccgaag atccacagaa acttaaaaca cccgctgata
    42721 tcatgccgct ctacctctgg ctgatgggcg atgacagccg ccgtaaaacc ggcatgacct
    42781 ttgacgccca accgggccgt aaaccaggaa tttcccaatg agtgatgaac gctaccaaca
    42841 gcgtcagcag cgagtgaaag aaaaagtaga tgctcgtgtg gcccaagccc aggatgaacg
    42901 cggtattatc atcgtcttta ccggcaatgg aaaaggcaaa accaccgcgg catttggtac
    42961 ggcaacacgc gcagttggtc acggaaaaaa agtgggcgtc gtgcagttta ttaaaggcac
    43021 ctggcctaat ggcgaacgca atctgctgga gccacatggc gttgagtttc aggtgatggc
    43081 aacgggcttt acctgggata cacaaaaccg cgagtctgat accgccgcct gccgcgaagt
    43141 ctggcaacat gcaaagcgga tgcttgctga ttcctcactg gatatggttt tgcttgatga
    43201 actgacgtat atggtggcgt atgactattt gccactggaa gaagtggtgc aggcgttaaa
    43261 tgaacgtcca catcaacaga cggtgattat cacgggtcga ggctgtcatc gggatattct
    43321 tgaactggcg gatacggtaa gtgagctgcg ccccatcaga catgcgtttg atgccggtgt
    43381 taaggcgcaa atagggattg attattaatc tgtaaaaatg ccggatatga cgtccggcat
    43441 actttgatta accgttatta cgcccgccag aacgacgact gccgctcacc tgactgtgac
    43501 gtttcaccgc acgacgaatc tgattagcct tcatgcgacg acggtctttt tctacagcga
    43561 ctttagagct ggtttctggc ggaagctcca ccagttcgcg cagatagtta gtctgggcga
    43621 ggtccagctc cgtccagcca ccgcgtggca gaccttttgg cagtgggata tcaccgtaac
    43681 gaacacggat caggcggctc acctgcacac caaccgcttc ccacagacga cgaacttcac
    43741 ggttacggcc ttcggtcaga gtcacgttgt accactggtt gatcccttcg ccgccgctga
    43801 acttgatggt tttaaaagct gccggaccat cttccagttg cacgccacga ctcaaatcac
    43861 gcagtttcgc gtcgtcaacc tgaccaaata cacgcacggc gtattcacgt tcaacttcac
    43921 ggcttggatg cattaaacgg tttgccagtt caccatcggt ggtgaacagc agcagaccac
    43981 aggtgttaac gtccagacga cctacggcaa tccagcgtgc gccacgcagc tttggcagac
    44041 ggtcaaacac cgtcggacgc ccttccggat cgttacgggt acacaactca ccttccggtt
    44101 tgtaataggc cagaacgcga caaatttgtt cagcggactc acgtaccgaa atcagatgac
    44161 catcgatacg aattttcagc ccaggggtaa cttcaacgcg atcgccgagt ttagcaattt
    44221 tgccatcaac actcacacga ccggcttcaa taatagattc gatttcacgg cgggagccgt
    44281 ggccagcgcg ggccagcact ttctgtaatt tttcgctcat agagcttcct tttggtgtcg
    44341 ccttcacagg cgtcgaacag gagaatcaat ggcgccgtat agcgccattt taaggccgcg
    44401 tagtatacag agttacgcac ttataagaaa ggcttcacat cacctacgcc ttcacgcacc
    44461 acgaccggcg tatcatcggt aagatcaata accgttgttg gtttctgccc gagatagccg
    44521 ccgtgaataa tcaaatctac ctgtttttcc agacggtctt taatttcttc gggatcggat
    44581 tcggtaaatt cgctgcctgg cagcattagc gaagtggaaa gcatcggttc gcccagtgct
    44641 tcaagcaacg cctgcgcaat cgggttagaa ggcacacgca taccgatggt tttgcgtttc
    44701 tcctgcaaca gacggcgtgg cacttccttc gtccccttca ggatgaaggt atagttgccc
    44761 ggcgtgttgt ttttcatcaa acgaaacgca acgttatcca cgaatgaata ggtcgagagt
    44821 tcagaaagat cgcgacacat cagggtaaag ttgtgaccgt ccggcagctg gcgaatacga
    44881 caaatacgct ccatggcgtt tttatcttca attttacagc cgagcgcata gccggaatca
    44941 gttggataga caatcacccc gcctttacgc acgatctcca ccgcctggtt gatcagacgt
    45001 tgctgcgggt tatcaggatg aatataaaaa aactggctca taattccctc tctgtggtgt
    45061 tctgcggctg ttcccatagc tgccagacgc cttcaacgcc tgcgggcagc cagagtttac
    45121 gacccagttc gatccacggg catggctgat gaaaatcaga tccttgtgat gcccataaat
    45181 gatgctgacg cgcaagtgtg gccagctggg tgcgttcatt gggcgattgc tggcactgcg
    45241 cgacttccat cgcgtcgccg tggtgttcgg caaaatgcgc aaccaatctt ttcagccatt
    45301 tagcactaag attgtaccgc ccaggatgag ctagcaccgc cttaccgcca gaatgatgaa
    45361 tgacatcaat agcttgttct attgtacacc actgtggcgg aacgtatccg gttttcccgc
    45421 gcgccagata ctttttaaag acatctgcca ttgaactggc tttgccgcac tccacaagga
    45481 aacgcgcaaa atgaccgcgc gtcactgcgc cgccctgcgc cagtcgttgt gcgccttcca
    45541 gcgccccagg tatttgcgct ttttcaagtc tttcggcaat ttgctgcgcc cgctgattgc
    45601 gccgttctgt ctgctgcgcg aggaactcac acattagcgg atgagtaata tcaatattca
    45661 gcccgacaat atgaatttca tgattttccc agaccgtgga aatttccacg ccgggaataa
    45721 gattcagcgc cagtcccgaa cgtgaaattt cctctcttgc tggcgcgatt gcagctgtgg
    45781 tgtcatggtc ggtgatcgcc agggtgccga cgcgcatctc gactgcacgg tgcaccaatg
    45841 cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg
    45901 cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc
    45961 ataacgggtc tggcaaatat tctgaaatga gctgttgaca attaatcatc gaaccagtta
    46021 actagtgcgc aagttcacgt aaaaagggta tcgacaatga aagcaatttt cgtactgaaa
    46081 ggttggtggc gcacttcctg aaacgggcag tgtattcacc atgcgtaaag caatcagata
    46141 cccagcccgc ctaatgagcg ggcttttttt tgaacaaaat tagagaataa caatgcaaac
    46201 acaaaaaccg actctcgaac tgctaacctg cgaaggcgct tatcgcgaca acccgactgc
    46261 gctttttcac cagttgtgtg ggaatcgtcc ggcaacgctg ctgctggaat ccgcagatat
    46321 cgacagcaaa gatgatttaa aaagcctgct gctgatagac agtgcgctgc gcattacagc
    46381 attaggtgac accgtcacaa ttcaggcact ttccggcaat ggcgaagccc tgctggcact
    46441 actggataac gccctgcctg cgggtgtgga aagtgaacaa tcaccaaact gccgtgtgct
    46501 gcgcttcccc cctgtcagtc cactgctgga tgaagacgcc cgcttatgct ccctttcggt
    46561 ttttgacgct ttccgtttat tgcagaatct gttgaatgta ccgaaggaag aacgagaagc
    46621 aatgttcttc ggcggcctgt tctcttatga ccttgtggcg ggatttgaag atttaccgca
    46681 actgtcagcg gaaaataact gccctgattt ctgtttttat ctcgctgaaa cgctgatggt
    46741 gattgaccat cagaaaaaaa gcacccgtat tcaggccagc ctgtttgctc cgaatgaaga
    46801 agaaaaacaa cgtctcactg ctcgcctgaa cgaactacgt cagcaactga ccgaagccgc
    46861 gccgccgctg ccagtggttt ccgtgccgca tatgcgttgt gaatgtaatc agagcgatga
    46921 agagttcggt ggcgtagtgc gtttgttgca aaaagcgatt cgcgctggag aaattttcca
    46981 ggtggtgcca tctcgccgtt tctctctgcc ctgcccgtca ccgctggcgg cctattacgt
    47041 gctgaaaaag agtaatccca gcccgtacat gttttttatg caggataatg atttcaccct
    47101 atttggcgcg tcgccggaaa gctcgctcaa gtatgatgcc accagccgcc agattgagat
    47161 ctacccgatt gccggaacac gcccacgcgg tcgtcgcgcc gatggttcac tggacagaga
    47221 tctcgacagc cgtattgaac tggaaatgcg tactgaccat aaagagctgt ctgaacatct
    47281 gatgctggtt gatctcgccc gtaatgatct ggcacgcatt tgcacccctg gcagccgcta
    47341 catcgccgat ctcaccaaag ttgaccgtta ttcctatgtg atgcacctcg tctctcgcgt
    47401 agtcggcgaa ctgcgtcacg atcttgacgc cctgcacgct tatcgcgcct gtatgaatat
    47461 ggggacgtta agcggtgcgc cgaaagtacg cgctatgcag ttaattgccg aggcggaagg
    47521 tcgtcgccgc ggcagctacg gcggcgcggt aggttatttc accgcgcatg gcgatctcga
    47581 cacctgcatt gtgatccgct cggcgctggt ggaaaacggt atcgccaccg tgcaagcggg
    47641 tgctggtgta gtccttgatt ctgttccgca gtcggaagcc gacgaaaccc gtaacaaagc
    47701 ccgcgctgta ctgtgcgcta ttgccaccgc gcatcatgca caggagactt tctgatggct
    47761 gacattctgc tgctcgataa tatcgactct tttacgtaca acctggcaga tcagttgcgc
    47821 agcaatggtc ataacgtggt gatttaccgc aaccatattc cggcgcagac cttaattgaa
    47881 cgcctggcga cgatgagcaa tccggtactg atgctttctc ctggccccgg tgtgccgagc
    47941 gaagccggtt gtatgccgga actcctcacc cgcttgcgtg gcaagctgcc cattattggc
    48001 atttgcctcg gacatcaggc aattgtcgaa gcttacgggg gctatgtcgg tcaggcgggc
    48061 gaaattcttc acggtaaagc gtcgagcatt gaacatgacg gtcaggcgat gtttgccgga
    48121 ttaacaaacc cgctgccggt ggcgcgttat cactcgctgg ttggcagtaa cattccggcc
    48181 ggtttaacca tcaacgccca ttttaatggc atggtgatgg cagtacgtca cgatgcggat
    48241 cgcgtttgtg gattccagtt ccatccggaa tccattctca ccacccaggg cgctcgcctg
    48301 ctggaacaaa cgctggcctg ggcgcagcag aaactagagc cagccaacac gctgcaaccg
    48361 attctggaaa aactgtatca ggcgcagacg cttagccaac aagaaagcca ccagctgttt
    48421 tcagcggtgg tgcgtggcga gctgaagccg gaacaactgg cggcggcgct ggtgagcatg
    48481 aaaattcgcg gtgagcaccc gaacgagatc gccggggcag caaccgcgct actggaaaac
    48541 gcagcgccgt tcccgcgccc ggattatctg tttgctgata tcgtcggtac tggcggtgac
    48601 ggcagcaaca gtatcaatat ttctaccgcc agtgcgtttg tcgccgcggc ctgtgggctg
    48661 aaagtggcga aacacggtaa ccgcagcgtc tccagtaaat ccggctcgtc ggatctgctg
    48721 gcggcattcg gtattaatct tgatatgaac gccaataaat cgcgccaggc gctggacgag
    48781 ttaggcgtat gtttcctctt tgcgccgaaa tatcacaccg gattccgcca cgcgatgccg
    48841 gttcgccagc aactgaaaac ccgcaccctg ttcaatgtgc tggggccatt gattaacccg
    48901 gcgcatccgc cgctggcgtt aattggtgtt tatagtccgg aactggtgct gccgattgcc
    48961 gaaaccttgc gcgtgctggg gtatcaacgc gcggcggtgg tgcacagcgg cgggatggat
    49021 gaagtttcat tacacgcgcc gacaatcgtt gccgagctgc atgacggcaa aattaagagc
    49081 tatcaattga ccgctgaaga ttttggcctg actccctacc accaggagca actggcaggc
    49141 ggaacacctg aagaaaaccg tgacatttta acacgcttgt tacaaggtaa aggcgacgcc
    49201 gcccatgaag cagccgtcgc tgcgaacgtc gccatgttaa tgcgcctgca tggccatgaa
    49261 gatctgcaag ccaatgcgca aaccgttctt gaggtactgc gcagtggttc cgcttacgac
    49321 agagttaccg cgctggcggc acgagggtaa atgatgcaaa ccgttttagc gaaaatcgtc
    49381 gcagacaagg cgatttgggt agaaacccgc aaacagcagc aaccgctggc cagttttcag
    49441 aatgaggttc agccgagcac acgacatttt tatgatgcgc tacagggtgc acgcacggcg
    49501 tttattctgg agtgtaaaaa agcgtcgccg tcaaaaggcg tgatccgtga tgatttcgat
    49561 ccggcacgca ttgccgccat ttataaacat tacgcttcgg caatttcggt gctgactgat
    49621 gagaaatatt ttcaggggag ctttgatttc ctcctcatcg tcagccaaat cgccccgcag
    49681 ccgattttat gtaaagactt cattatcgac ccttaccaga tctatctggc gcgctattac
    49741 caggccgatg cctgcttatt aatgctttca gtactggatg acgaccaata tcgccagctt
    49801 gcagccgtcg ctcacagtct ggagatgggg gtgctgaccg aagtcagtaa tgaagaggaa
    49861 ctggagcgcg ccattgcatt aggggcaaag gtcgttggca tcaacaaccg cgatctgcgt
    49921 gatttgtcga ttgatctcaa ccgtacccgc gagcttgcgc cgaaactggg gcacaacgta
    49981 acggtaatca gcgaatccgg catcaatact tacgctcagg tgcgcgagtt aagccacttc
    50041 gctaacggtt ttctgattgg ttcggcgttg atggcccatg acgatttgca cgccgccgtg
    50101 cgccgggtgt tgctgggtga gaataaagta tgtggcctga cgcgtgggca agatgctaaa
    50161 gcagcttatg acgcgggcgc gatttacggt gggttgattt ttgttgcgac atcaccgcgt
    50221 tgcgtcaacg ttgaacaggc gcaggaagtg atggctgcgg caccgttgca gtatgttggc
    50281 gtgttccgca atcacgatat tgccgatgtg gtggacaaag ctaaggtgtt atcgctggcg
    50341 gcagtgcaac tgcatggtaa tgaagatcag ctgtatatcg atacgctgcg tgaagctctg
    50401 ccagcacatg ttgccatctg gaaagcatta agcgtcggtg aaaccctgcc cgcccgcgag
    50461 tttcagcacg ttgataaata tgttttagac aacggccagg gtggaagcgg gcaacgtttt
    50521 gactggtcac tattaaatgg tcaatcgctt ggcaacgttc tgctggcggg gggcttaggc
    50581 gcagataact gcgtggaagc ggcacaaacc ggctgcgccg gacttgattt taattctgct
    50641 gtagagtcgc aaccgggcat caaagacgca cgtcttttgg cctcggtttt ccagacgctg
    50701 cgcgcatatt aaggaaagga acaatgacaa cattacttaa cccctatttt ggtgagtttg
    50761 gcggcatgta cgtgccacaa atcctgatgc ctgctctgcg ccagctggaa gaagcttttg
    50821 tcagcgcgca aaaagatcct gaatttcaag cccagttcaa cgacctgctg aaaaactatg
    50881 ccgggcgtcc aaccgcactg accaaatgcc agaacattac agccgggacg aacaccacgc
    50941 tgtatctcaa gcgtgaagat ttgctgcacg gcggcgcaca taaaaccaac caggtgctgg
    51001 ggcaggcgtt gctggcgaag cggatgggta aaaccgaaat catcgccgaa accggggccg
    51061 gtcagcatgg cgtggcgtcg gcccttgcca gcgccctgct cggcctgaaa tgccgtattt
    51121 atatgggtgc caaagacgtt gaacgccagt cgcctaacgt ttttcgtatg cgcttaatgg
    51181 gtgcggaagt gatcccggta catagcggtt ccgccacgct gaaagatgcc tgtaatgagg
    51241 cgctacgcga ctggtccggc agttatgaaa ccgcgcacta tatgctgggc accgcggctg
    51301 gcccgcatcc ttatccgacc attgtccgtg agtttcagcg gatgattggc gaagaaacca
    51361 aagcgcagat tctggaaaga gaaggtcgcc tgccggatgc cgttatcgcc tgtgttggcg
    51421 gcggttcgaa tgccatcggc atgtttgctg atttcatcaa tgaaaccaac gtcggcctga
    51481 ttggtgtgga gcctggtggt cacggtatcg aaactggcga gcacggcgca ccgctaaaac
    51541 atggtcgcgt gggcatttat ttcggtatga aagcgccgat gatgcaaacc gaagacgggc
    51601 agattgaaga atcttactcc atctccgccg gactggattt cccgtctgtc ggtccacaac
    51661 acgcgtatct taacagcact ggacgcgctg attacgtgtc tattaccgat gatgaagccc
    51721 ttgaagcctt caaaacgctg tgcctgcacg aagggatcat cccggcgctg gaatcctccc
    51781 acgccctggc ccatgcgctg aaaatgatgc gcgaaaaccc ggataaagag cagctactgg
    51841 tggttaacct ttccggtcgc ggcgataaag acatcttcac cgttcacgat attttgaaag
    51901 cacgagggga aatctgatgg aacgctacga atctctgttt gcccagttga aggagcgcaa
    51961 agaaggcgca ttcgttcctt tcgtcacgct cggtgatccg ggcattgagc agtcattgaa
    52021 aattatcgat acgctaattg aagccggtgc tgacgcgctg gagttaggta tccccttctc
    52081 cgacccactg gcggatggcc cgaccattca aaacgccact ctgcgcgcct ttgcggcagg
    52141 tgtgactccg gcacaatgtt ttgaaatgct ggcactgatt cgccagaaac acccgaccat
    52201 tcccattggc ctgttgatgt atgccaatct ggtgtttaac aaaggcattg atgagtttta
    52261 tgcccagtgc gagaaagtcg gcgtcgattc ggtgctggtt gccgatgtgc cagttgaaga
    52321 gtccgcgccc ttccgccagg ccgcgttgcg ccataatgtc gcacctatct tcatctgccc
    52381 gccaaatgcc gatgacgacc tgctgcgcca gatagcctct tacggtcgtg gttacacata
    52441 tttgctgtca cgagcgggcg tgaccggcgc agaaaaccgc gccgcgttac ccctcaatca
    52501 tctggttgcg aagctgaaag agtacaacgc tgcacctcca ttgcaggggt ttggtatttc
    52561 cgccccggat caggtaaaag cagcgattga tgcaggagct gcgggcgcga tttctggttc
    52621 ggccattgtt aaaatcatcg agcaacatat taatgagcca gagaaaatgc tggcggcact
    52681 gaaagttttt gtacaaccga tgaaagcggc gacgcgcagt taatcccaca gccgccagtt
    52741 ccgctggcgg cattttaact ttctttaatg aagccggaaa aatcctaaat tcatttaata
    52801 tttatctttt taccgtttcg cttaccccgg tcgaacgtca acttacgtca tttttccgcc
    52861 caacagtaat ataatcaaac aaattaatcc cgcaacataa caccagtaaa atcaataatt
    52921 ttctctaagt cacatattcc tcaggtaatt gttaatatat ccagaatgtt cctcaaaata
    52981 tattttccct ctatcttctc gttgcgctta atttgactaa ttctcattag cgactaattt
    53041 taatgagtgt cgacacacaa cactcatatt aatgaaacaa tgcaacgcaa cgggagaaat
    53101 aacatggccg aacatcgtgg tagttcagga aatttcgccg aagaccgtga gaaggcatcc
    53161 gacgcaggcc gtaaaggcgg tcagcatagc ggcggtaatt ttaaaaatga tccgcaacgc
    53221 gcatctgaag cgggtaaaaa aggcggtcaa caaagcggtg gtaataaatc aggcaaatcc
    53281 tgattcaccg ttaattatta atatcagctg ataaagataa ttgcatctgc gggccgtatg
    53341 gctcgcagtg atttcactgg agaaaaatat gaatatgaag accattgaag atgtatttat
    53401 tcaactgctt tcagatacct acagcgcaga aaaacaatta acccgggcac tggcaaaact
    53461 cgcaagagca acatcaaatg aaaaattaag tcaggctttt catgcgcacc tcgaggaaac
    53521 tcatggacag attgaacgaa ttgatcaagt tgtggaatca gaatcaaatc tgaaaattaa
    53581 gcgcatgaaa tgtgtggcaa tggaaggtct tattgaagaa gctaatgagg tcatcgaaag
    53641 taccgagaaa aacgaagtgc gtgatgccgc actgattgcc gcagcacaga aagtcgagca
    53701 ttatgagatt gccagttacg ggacattagc gacgctggct gaacaattag gttaccgtaa
    53761 agcagcgaag cttctgaaag aaaccctgga agaagaaaag gccaccgaca tcaaactgac
    53821 tgatctggcc cttaataacg taaataagaa agccgaaaat aaagcctgaa atatgaattt
    53881 taacttttag tcattttata aagaggacat tttcatgaat cgtattgaac attatcatga
    53941 ctggttacgt gacgcccacg caatggaaaa gcaagccgaa tctatgcttg aatccatggc
    54001 cagccgtata gataattatc ctgaactacg cgctcgtatt gaacaacatc ttagtgaaac
    54061 caaaaaccag attgttcaac tggaaactat tcttgatcgt aatgacattt cacgttcagt
    54121 cattaaagat tccatgagta aaatggctgc gcttgggcag tcaatcggtg gtatattccc
    54181 ttctgatgaa atagttaaag gctctattgg cggatatgtc ttcgagcaat ttgaaatcac
    54241 ctgttacacc tcactattag cagcagcaaa aaatgccggt gatacagtct caattccgat
    54301 catcgaagcg attttaaatg aggaaaagca aatggccgac tggctgattc agcatattcc
    54361 gcaaacaact gagaaatttt taattcgttc tgaaactgat ggcgtagaag cgaagaaata
    54421 atacccttta taccatgtcc ttattgaccc cgtatattac ggggtcgttt ttgtgcggaa
    54481 ttaaaaacga tatcctgctg agaacataaa cacccacgga tcgaggcgta cgctatcgtg
    54541 ttgctgtgca ccgcctagct tataattggc ggtggtatcg atatccatgt accacactga
    54601 catgttaacc aaccagtcac ggttaatcag ataatcaacc cccacctgcc cggcagctcc
    54661 ccaggaatct ttcagactga gatcggaaag ccccgcctct ttgccatgat cgttaaatcc
    54721 attatcaaag aaggtggtgt agttaatacc ggccccaacg taaggacgga atttgctgct
    54781 ggcatcacca aaataccact gcgccatcag tgttggcggc agatggtgaa cggttgcaat
    54841 atcgccggtc gcccgggtgc cgattttatg gcggaacggc gtcgctgcca gtaattccac
    54901 accaatgttg tcggtcgcca tataagtaaa cgtcaagccc agttgagtgt tattggtcac
    54961 gctgaatcca cccagacttc ctaacgtacc accagcacct tctgttggac gtacggttgc
    55021 agaacctgca cgcataaaaa attcgcctgc ttcatgcgca aaggcactgc cagaaagaag
    55081 agttgttact gccaaagccg ccactgttaa ctttttcata tccgctccgt cgttatggtt
    55141 ataaaaacgt gacgaatata cctacattgg agtaacaaat gatcctacat agatcacatt
    55201 acatatgata attcaccatt tattgatctg gattaattta gaagttgcat tgagaatcct
    55261 ggttaatgtg atttaaatca atttttaaat attttctcta cgcaaatatt aactcgatcg
    55321 ccacttgccg cggtttgccc acgcctgaag gccagcaatt acctccgctt tacaaagata
    55381 tgcttttcca tacaagacct gatgctgcca cccgaggctt aaccagggta caattgcccg
    55441 ctaattaaca cctgcataaa actcaaggag agtgcatgtc tatcacggcg cagtccgtat
    55501 accgtgacac cggaaatttc ttccgtaatc aatttatgac cattctgttg gtatcgttgc
    55561 tatgtgcgtt tatcacagtg gtgttagggc atgttttctc acccagtgat gcacagcttg
    55621 cgcagctcaa tgacggcgtg cccgttagcg gcagtagtgg gttgttcgac ctggttcaga
    55681 atatgtcacc ggaacagcaa caaattttgc tgcaggcttc agcggcgtcc actttttcag
    55741 aattaatcgg taacgccatt ctcgccggag gcgtaatatt aattatccag ctggtgtctg
    55801 cgggtcagag agtcagtgcg ctgcgagcta ttggtgccag tgcgccgata ttgcccaagt
    55861 tatttattct gatttttctg actacccttt tagtacagat tggcatcatg ctggtggtcg
    55921 ttccgggaat tatcatggcc attttactgg ccctggcacc ggtgatgctg gtacaggaca
    55981 aaatgggcat ttttgcctcg atgcgtagca gtatgcggct gacttgggcg aatatgcgtc
    56041 tggtggcacc cgcagtactg agctggttgc tggcaaaaac actgttgctg ctttttgcct
    56101 cttcttttgc cgcattaacc ccggaaattg gtgccgtact ggcgaacacc ttgagcaacc
    56161 tgatttctgc catattgctc atctatctgt tccgcctgta tatgttgatt cgccaataac
    56221 cttaagcatt tgattacgga atcgtaaaat gaagcagttt cttgattttt taccgctggt
    56281 tgtctttttc gcgttttaca agatttatga catctatgcg gctactgcag cgctgatcgt
    56341 cgccacggcg attgtgctta tctatagctg ggttcgcttt cgtaaggttg agaagatggc
    56401 cctgatcact tttgttctgg tggtcgtctt cggtggcttg acgctgttct tccacaatga
    56461 tgagtttatt aaatggaagg ttacagtcat ttatgctctg tttgcgggag ccctgttagt
    56521 cagccaatgg gtgatgaaaa agccgctaat tcagcggatg ctgagtaaag aactcacgct
    56581 gccgcaaccg gtatggtcga agctaaatct ggcctgggct gttttcttta tcctttgcgg
    56641 tctggcaaac atctacatcg cattctggct gccgcaaaat atttgggtca actttaaagt
    56701 ctttggcctg accgccctta ccttaatctt cacattgtta agcggtatct atatctaccg
    56761 ccacatgccg caggaagata aatcctaacc agactgccag tccttcgggg ctggcgctgt
    56821 atctcctctg ttctcctcag gaaaatcata gtagcatcgc gcctgtgatt ttccttttaa
    56881 gtcggtttta ccatgtctac aacacataac gtccctcagg gcgatcttgt tttacgtact
    56941 ttagccatgc ccgccgatac caatgccaat ggtgacatct ttggtggttg gttaatgtca
    57001 caaatggata ttggcggcgc tattctggca aaagaaattg cccacggtcg cgtagtgact
    57061 gtgcgggttg aaggaatgac tttcttacgg ccggttgcgg tcggcgatgt ggtgtgctgc
    57121 tatgcacgct gtgtccagaa agggacgaca tcggtcagca ttaatattga agtatgggtg
    57181 aaaaaagtag cgtctgaacc aatcgggcaa cgctataaag cgacagaagc attatttaag
    57241 tatgtagcgg ttgatcctga aggaaaacct cgcgccttac ctgttgagta atagtcaaaa
    57301 gcctccggtc ggaggctttt gactttctgc ttactgaatt tcggtggtgc cgttaatttt
    57361 aaacaggata ttcaccacaa tcccactgcc tggcttaccc ggctcataac gccatctgcg
    57421 catcgcattt ttcacctcac gctcaaacat gttcgcaggc ttggctgaga ggatttgtac
    57481 gttatccacg cgaccatccg gcgtgacgtc aaatttaact ttaacctgcc cttcaatgcg
    57541 caatgcctgt gctcgtgccg gatactgcgg ctgattacgg cttaatgcgc gtggtcctga
    57601 agccacactg gtaaccggct tgctggttgc agccgttgcc gtacttgatg tggggcgcgc
    57661 gggtgccgta ttttcaaacg gtgatgccgg acgcgactct acgggtttga catcgcgttt
    57721 tggctgctcc tgtacctttt tcaccggctt cggttttggc ttcggctttt caatgaccac
    57781 cggtgcttct tttggcggtt cggggatcgg ctcaggttcc ggttctggct ctaccaccgg
    57841 ctccggtggc ggctgaacgg cttgtggcgg ttcgagatca gcaggcgcaa ccatcgtgac
    57901 agaaattggt tgcgcaggcg caggtagttc aataacctga tgtaccgagg tatagagcag
    57961 ccccgccaca acagcaccat gaatgcagac cgaaagtaac gtcggccagg ggaagcggcg
    58021 aggtaaatca agggttattg aagtcataat catttcagta gaaaaactag gcctcgattt
    58081 taaatgcaaa tagcaatcat attcaataag gcaagacgat caggatcagt gaaaatgtca
    58141 tttttggcgt aattaccctt actttatgca tgaccttaac aaagacatca tctttccgtt
    58201 gcagatcttc gccctttcaa ataacgtact ttacaacttt cccgaacaag gagttgtgcc
    58261 cgtgttgtat gttatttacg ctcaagacaa agctgatagc ctcgaaaaac gcctttccgt
    58321 tcgtccggca catttagcac gtttacagtt actgcatgat gaaggtcgtt tgttgacagc
    58381 tggtccaatg ccagcggtag acagtaacga tccaggggct gcgggattta ctggctcaac
    58441 cgttattgct gaatttgaat cgctggaagc agctcaggcc tgggccgatg ctgacccgta
    58501 cgtagcggca ggagtctatg aacacgtttc agtgaaacca tttaagaaag tgttctgag
//
LOCUS       DAOFMX010000015        58547 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.015, whole genome shotgun sequence.
ACCESSION   DAOFMX010000015 DAOFMX010000000
VERSION     DAOFMX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.015"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            120..2654
                     /gene="mrcB"
                     /locus_tag="QBA20_001065"
     CDS             120..2654
                     /gene="mrcB"
                     /locus_tag="QBA20_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDQ6291692.1"
                     /translation="MAGNDREPIGRKGKLTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2874..5117
                     /gene="fhuA"
                     /locus_tag="QBA20_001066"
     CDS             2874..5117
                     /gene="fhuA"
                     /locus_tag="QBA20_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDQ6291693.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5168..5965
                     /gene="fhuC"
                     /locus_tag="QBA20_001067"
     CDS             5168..5965
                     /gene="fhuC"
                     /locus_tag="QBA20_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDQ6291694.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5965..6855
                     /gene="fhuD"
                     /locus_tag="QBA20_001068"
     CDS             5965..6855
                     /gene="fhuD"
                     /locus_tag="QBA20_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDQ6291695.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELMTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6852..8834
                     /gene="fhuB"
                     /locus_tag="QBA20_001069"
     CDS             6852..8834
                     /gene="fhuB"
                     /locus_tag="QBA20_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDQ6291696.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8869..10149)
                     /gene="hemL"
                     /locus_tag="QBA20_001070"
     CDS             complement(8869..10149)
                     /gene="hemL"
                     /locus_tag="QBA20_001070"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDQ6291697.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10374..11795
                     /gene="clcA"
                     /locus_tag="QBA20_001071"
     CDS             10374..11795
                     /gene="clcA"
                     /locus_tag="QBA20_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDQ6291698.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11819..11884
                     /locus_tag="QBA20_001072"
     CDS             11819..11884
                     /locus_tag="QBA20_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291699.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            11877..12221
                     /gene="erpA"
                     /locus_tag="QBA20_001073"
     CDS             11877..12221
                     /gene="erpA"
                     /locus_tag="QBA20_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDQ6291700.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12268..12891)
                     /locus_tag="QBA20_001074"
     CDS             complement(12268..12891)
                     /locus_tag="QBA20_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDQ6291701.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12929..13729)
                     /gene="btuF"
                     /locus_tag="QBA20_001075"
     CDS             complement(12929..13729)
                     /gene="btuF"
                     /locus_tag="QBA20_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDQ6291702.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13722..14420)
                     /gene="mtnN"
                     /locus_tag="QBA20_001076"
     CDS             complement(13722..14420)
                     /gene="mtnN"
                     /locus_tag="QBA20_001076"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDQ6291703.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14504..16021
                     /gene="dgt"
                     /locus_tag="QBA20_001077"
     CDS             14504..16021
                     /gene="dgt"
                     /locus_tag="QBA20_001077"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDQ6291704.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16151..17575
                     /gene="degP"
                     /locus_tag="QBA20_001078"
     CDS             16151..17575
                     /gene="degP"
                     /locus_tag="QBA20_001078"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDQ6291705.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            17730..18886
                     /gene="cdaR"
                     /locus_tag="QBA20_001079"
                     /pseudo
     CDS             17730..18886
                     /gene="cdaR"
                     /locus_tag="QBA20_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(18975..19361)
                     /locus_tag="QBA20_001080"
     CDS             complement(18975..19361)
                     /locus_tag="QBA20_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDQ6291706.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19675..20499)
                     /gene="dapD"
                     /locus_tag="QBA20_001081"
     CDS             complement(19675..20499)
                     /gene="dapD"
                     /locus_tag="QBA20_001081"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDQ6291707.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20530..23202)
                     /gene="glnD"
                     /locus_tag="QBA20_001082"
     CDS             complement(20530..23202)
                     /gene="glnD"
                     /locus_tag="QBA20_001082"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDQ6291708.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVHQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23264..24058)
                     /gene="map"
                     /locus_tag="QBA20_001083"
     CDS             complement(23264..24058)
                     /gene="map"
                     /locus_tag="QBA20_001083"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDQ6291709.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24426..25151
                     /gene="rpsB"
                     /locus_tag="QBA20_001084"
     CDS             24426..25151
                     /gene="rpsB"
                     /locus_tag="QBA20_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDQ6291710.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25409..26260
                     /gene="tsf"
                     /locus_tag="QBA20_001085"
     CDS             25409..26260
                     /gene="tsf"
                     /locus_tag="QBA20_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDQ6291711.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26407..27132
                     /gene="pyrH"
                     /locus_tag="QBA20_001086"
     CDS             26407..27132
                     /gene="pyrH"
                     /locus_tag="QBA20_001086"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDQ6291712.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27424..27981
                     /gene="frr"
                     /locus_tag="QBA20_001087"
     CDS             27424..27981
                     /gene="frr"
                     /locus_tag="QBA20_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDQ6291713.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28073..29269
                     /gene="ispC"
                     /locus_tag="QBA20_001088"
     CDS             28073..29269
                     /gene="ispC"
                     /locus_tag="QBA20_001088"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDQ6291714.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            29458..30216
                     /gene="ispU"
                     /locus_tag="QBA20_001089"
                     /gene_synonym="uppS"
     CDS             29458..30216
                     /gene="ispU"
                     /locus_tag="QBA20_001089"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDQ6291715.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30229..31086
                     /gene="cdsA"
                     /locus_tag="QBA20_001090"
     CDS             30229..31086
                     /gene="cdsA"
                     /locus_tag="QBA20_001090"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDQ6291716.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31098..32450
                     /gene="rseP"
                     /locus_tag="QBA20_001091"
     CDS             31098..32450
                     /gene="rseP"
                     /locus_tag="QBA20_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDQ6291717.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32480..34912
                     /gene="bamA"
                     /locus_tag="QBA20_001092"
     CDS             32480..34912
                     /gene="bamA"
                     /locus_tag="QBA20_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDQ6291718.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            35034..35519
                     /gene="skp"
                     /locus_tag="QBA20_001093"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             35034..35519
                     /gene="skp"
                     /locus_tag="QBA20_001093"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDQ6291719.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35523..36548
                     /gene="lpxD"
                     /locus_tag="QBA20_001094"
     CDS             35523..36548
                     /gene="lpxD"
                     /locus_tag="QBA20_001094"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDQ6291720.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQSNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36653..37108
                     /gene="fabZ"
                     /locus_tag="QBA20_001095"
     CDS             36653..37108
                     /gene="fabZ"
                     /locus_tag="QBA20_001095"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDQ6291721.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37112..37900
                     /gene="lpxA"
                     /locus_tag="QBA20_001096"
     CDS             37112..37900
                     /gene="lpxA"
                     /locus_tag="QBA20_001096"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDQ6291722.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37900..39048
                     /gene="lpxB"
                     /locus_tag="QBA20_001097"
     CDS             37900..39048
                     /gene="lpxB"
                     /locus_tag="QBA20_001097"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDQ6291723.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            39045..39641
                     /gene="rnhB"
                     /locus_tag="QBA20_001098"
     CDS             39045..39641
                     /gene="rnhB"
                     /locus_tag="QBA20_001098"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDQ6291724.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            39678..43160
                     /gene="dnaE"
                     /locus_tag="QBA20_001099"
     CDS             39678..43160
                     /gene="dnaE"
                     /locus_tag="QBA20_001099"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDQ6291725.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPSLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            43173..44132
                     /gene="accA"
                     /locus_tag="QBA20_001100"
     CDS             43173..44132
                     /gene="accA"
                     /locus_tag="QBA20_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDQ6291726.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44231..46372
                     /gene="ldcC"
                     /locus_tag="QBA20_001101"
     CDS             44231..46372
                     /gene="ldcC"
                     /locus_tag="QBA20_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDQ6291727.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            46429..46818
                     /locus_tag="QBA20_001102"
     CDS             46429..46818
                     /locus_tag="QBA20_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDQ6291728.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            46883..48181
                     /gene="tilS"
                     /locus_tag="QBA20_001103"
     CDS             46883..48181
                     /gene="tilS"
                     /locus_tag="QBA20_001103"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDQ6291729.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(48230..48490)
                     /gene="rof"
                     /locus_tag="QBA20_001104"
     CDS             complement(48230..48490)
                     /gene="rof"
                     /locus_tag="QBA20_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDQ6291730.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(48477..48677)
                     /locus_tag="QBA20_001105"
     CDS             complement(48477..48677)
                     /locus_tag="QBA20_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDQ6291731.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            48843..49388
                     /locus_tag="QBA20_001106"
     CDS             48843..49388
                     /locus_tag="QBA20_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDQ6291732.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            49385..49807
                     /gene="arfB"
                     /locus_tag="QBA20_001107"
     CDS             49385..49807
                     /gene="arfB"
                     /locus_tag="QBA20_001107"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDQ6291733.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            49821..50531
                     /gene="nlpE"
                     /locus_tag="QBA20_001108"
                     /gene_synonym="cutF"
     CDS             49821..50531
                     /gene="nlpE"
                     /locus_tag="QBA20_001108"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDQ6291734.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(50686..51510)
                     /locus_tag="QBA20_001109"
     CDS             complement(50686..51510)
                     /locus_tag="QBA20_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HDQ6291735.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(51563..53281)
                     /gene="proS"
                     /locus_tag="QBA20_001110"
     CDS             complement(51563..53281)
                     /gene="proS"
                     /locus_tag="QBA20_001110"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDQ6291736.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKELRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(53392..54099)
                     /gene="tsaA"
                     /locus_tag="QBA20_001111"
     CDS             complement(53392..54099)
                     /gene="tsaA"
                     /locus_tag="QBA20_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDQ6291737.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(54096..54500)
                     /gene="rcsF"
                     /locus_tag="QBA20_001112"
     CDS             complement(54096..54500)
                     /gene="rcsF"
                     /locus_tag="QBA20_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDQ6291738.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(54618..55433)
                     /gene="metQ"
                     /locus_tag="QBA20_001113"
     CDS             complement(54618..55433)
                     /gene="metQ"
                     /locus_tag="QBA20_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDQ6291739.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LIAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(55473..56126)
                     /gene="metI"
                     /locus_tag="QBA20_001114"
     CDS             complement(55473..56126)
                     /gene="metI"
                     /locus_tag="QBA20_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDQ6291740.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(56119..57150)
                     /gene="metN"
                     /locus_tag="QBA20_001115"
     CDS             complement(56119..57150)
                     /gene="metN"
                     /locus_tag="QBA20_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDQ6291741.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            57338..57913
                     /gene="gmhB"
                     /locus_tag="QBA20_001116"
     CDS             57338..57913
                     /gene="gmhB"
                     /locus_tag="QBA20_001116"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDQ6291742.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            58277..>58547
                     /locus_tag="QBA20_001117"
     rRNA            58277..>58547
                     /locus_tag="QBA20_001117"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        13912 a        14414 c        16107 g        14114 t
ORIGIN      
        1 cctctccctc aagggagagg ggaccgatcg agcgcaaatt ttgagagata tcttcttctg
       61 tcttgtaaca gaagaacaga aaatcgggct tttgcgcctg aatattgcgg agaaaaagca
      121 tggccgggaa tgaccgcgag ccaattggac gcaaagggaa actgacgcgt ccggtcaaac
      181 aaaaggtaag ccgtcgtcgt tacgaagatg acgatgatta cgacgattat gatgactatg
      241 aggatgaaga accgatgccg cgcaaaggta agggcaaagg caaagggcgt aagcctcgtg
      301 gcaaacgcgg ctggctatgg ctactgctaa aactggctat cgtttttgcc gtgctgatcg
      361 ccatttacgg cgtttatctc gatcaaaaaa ttcgtagccg tattgatggc aaggtctggc
      421 aactgcccgc ggcagtttat ggccgaatgg tcaaccttga accagatatg accatcagca
      481 agaacgaaat ggtgaagctg ctggaggcaa cacagtatcg tcaggtgtcg aaaatgaccc
      541 gtcctggcga atttaccgtg caggccaaca gcattgagat gatccgccgt ccgtttgatt
      601 tcccggacag taaagaaggg caggtgcgtg cgcgtctgac ctttgatggt gatcatctgg
      661 cgaccatcgt caatatggag aacaaccgtc agttcggttt cttccgtctt gatccgcgtc
      721 tgatcaccat gatctcctcg ccaaacggtg agcagcgttt gtttgtgccg cgcagtggtt
      781 tccctgattt gctggtggat actttgctgg cgacagaaga ccgtcatttt tacgagcatg
      841 atggaatcag tctctactcc attgggcgtg cggtgctggc aaacctgacc gcaggtcgca
      901 cggtgcaggg tgcgagtacg ctgacgcaac agctggtgaa aaacctgttc ctctccagcg
      961 agcgttctta ctggcgtaaa gcgaacgaag cttacatggc gctgatcatg gacgcgcgtt
     1021 acagcaaaga ccgtattctt gagctgtata tgaacgaggt gtatctcggt cagagcggcg
     1081 acaacgaaat ccgcggcttc ccgctggcaa gcttgtatta ctttggtcgc ccggtagaag
     1141 agctaagcct cgaccagcag gcgctgttag tcggtatggt gaaaggggcg tccatctaca
     1201 acccgtggcg taacccgaaa ctggcgctgg agcgacgtaa tctggtgctg cgtctgctgc
     1261 aacagcaaca gattattgat caagaactct atgacatgtt gagtgcccgt ccgctggggg
     1321 ttcagccgcg tggtggggtg atctctcctc agccagcctt tatgcagctg gtgcgtcagg
     1381 agttgcaggc aaaactgggc gataaggtaa aagatctctc cggcgtgaag atcttcacta
     1441 cctttgactc ggtggcccag gacgcggcag aaaaagccgc cgtggaaggc attccggcac
     1501 tgaagaaaca gcgtaagttg agcgatcttg aaactgcgat tgtggtcgtc gaccgcttta
     1561 gtggtgaagt tcgtgcgatg gtcggaggtt ctgagccgca gtttgcgggc tacaaccgtg
     1621 cgatgcaggc gcgtcgttcg attggttccc ttgcaaaacc agcgacttat ctgacggctt
     1681 taagccagcc gaaaatctat cgcctgaaca cgtggattgc cgatgcgcca atcgcgctgc
     1741 gtcagccgaa tggccaggtc tggtcaccgc agaacgatga ccgtcgttat agcgaaagcg
     1801 gcagagtgat gctggtggat gcgttgacac gttcgatgaa cgtgccgacg gtaaatctgg
     1861 ggatggcgct ggggctgcct gcggttacgg agacctggat caaactgggc gtaccgaaag
     1921 atcagttgca tccggttccg gcaatgctgc tgggggcgtt gaacttaacg ccaatcgaag
     1981 tggcgcaggc attccagacc atcgccagcg gtggtaaccg tgcaccgctt tctgcactgc
     2041 gttcggtaat cgcggaagat ggtaaggtgc tgtatcagag cttcccgcag gcagaacgcg
     2101 ctgttccggc gcaggcggcg tatctgacac tatggaccat gcagcaggtg gtacaacgtg
     2161 gtacgggtcg tcagcttggg gcgaaatacc cgaacctgca tctggcaggg aaaacaggga
     2221 ctaccaacaa taacgtagat acctggtttg caggcattga cggcagcacg gtgaccatca
     2281 cctgggtagg ccgtgataac aaccagccga ccaaactgta tggagccagc ggggcaatgt
     2341 cgatttatca gcgttatctg gctaaccaga cgccaacgcc gctgaatctt gttccgccag
     2401 aagatattgc agatatgggc gtggactacg acggcaactt tgtttgcagc ggtggcatgc
     2461 gtgtcttgcc ggtctggacc agcgatccgc aatcgctgtg ccagcagagc gagatgcagc
     2521 agcagccgtc aggcaatccg tttgatcagt cttctcagcc gcagcaacag ccgcaacagc
     2581 aacctgctca gcaagagcag aaagacagcg acggtgtagc cggttggatc aaggatatgt
     2641 ttggtagtaa ttaacatcta agcgtgaaat accggatggc gagttgccat ccggtaaaat
     2701 aacatcccat ctaagatatt aaccctttct tttcatctgg ttgtttatta acccttcagg
     2761 aacgctcaga ttgcgtaccg cttgcgaacc cgccagcgtt tcgaatatta tcttatcttt
     2821 ataataatca ttctcgttta cgttattatt cactttacat cagagatata ccaatggcgc
     2881 gttccaaaac tgctcagcca aaacactcac tgcgtaaaat cgcagttgta gtagccacag
     2941 cggttagcgg catgtctgtt tatgcacagg cagcggttga accgaaagaa gacactatca
     3001 ccgttaccgc tgcacctgcg ccgcaagaaa gcgcatgggg gcctgctgca actattgcgg
     3061 cgcggcagtc agctaccggc actaagaccg atacgccgat tcaaaaagtg ccacagtcta
     3121 tttctgttgt gaccgccgaa gagatggcgc tgcatcagcc gaagtcggta aaagaagcgc
     3181 ttagctacac gccgggtgtc tctgttggta cgcgtggcgc atccaacacc tatgaccacc
     3241 tgatcattcg cggttttgcg gcagaaggcc aaagccaaaa taactatctg aatggcctga
     3301 agttgcaggg caacttctat aacgatgcgg tcattgaccc gtatatgctg gaacgcgctg
     3361 aaattatgcg tggcccggtt tccgtgcttt acggtaaaag cagtcctggc ggtctgttga
     3421 atatggtcag caagcgtccg accaccgaac cgctgaaaga agttcagttt aaagccggta
     3481 ctgacagcct gttccagact ggttttgact ttagcgatgc gttggatgat gacggtgttt
     3541 actcttatcg cctgaccggt cttgcgcgtt ctgccaatgc ccagcagaaa gggtcagaag
     3601 agcagcgtta tgctattgca ccggcgttca cctggcgtcc ggatgataaa accaatttca
     3661 ccttcctttc ttacttccag aacgagccgg aaaccggtta ttacggctgg ttgccgaaag
     3721 agggaaccgt tgagccgctg ccgaacggta agcgtctgcc gacagacttt aatgaagggg
     3781 cgaagaacaa cacctattct cgtaatgaga agatggtcgg ctacagcttc gatcacgaat
     3841 ttaacgacac ctttactgtg cgtcagaacc tgcgctttgc tgaaaacaaa acctcgcaaa
     3901 acagcgttta tggttacggc gtctgctccg atccggcgaa tgcttacagc aaacagtgtg
     3961 cggcattagc gccagcggat aaaggccatt atctggcacg taaatacgtc gttgatgatg
     4021 agaagctgca aaacttctcc gttgataccc agttgcagag caagtttgcc actggcgata
     4081 tcgaccacac cctgctgacc ggtgtcgact ttatgcgtat gcgtaatgac atcaacgcct
     4141 ggtttggtta cgacgactct gtgccactgc tcaatctgta caatccggtg aatcccgatt
     4201 tcgacttcaa tgccaaagat ccggcaaact ccggccctta ccgcattctg aataaacaga
     4261 aacaaacggg cgtttatgtt caggatcagg cgcagtggga taaagtgctg gtcaccctag
     4321 gcggtcgtta tgactgggca gatcaagaat ctcttaaccg cgttgctggg acgaccgata
     4381 aacgtgatga caaacagttt acctggcgtg gtggtgttaa ctacctgttt gataatggtg
     4441 taacacctta cttcagctat agcgaatcgt ttgaaccttc ttcgcaagtt gggaaggatg
     4501 gtaatatttt cgcaccgtct aaaggtaagc agtatgaagt cggcgtgaaa tatgtaccgg
     4561 aagatcgtcc gattgtagtt actggtgccg tgtataatct cactaaaacc aacaacctga
     4621 tggcggaccc tgagggttcc ttcttctcgg ttgaaggtgg cgagatccgc gcacgtggcg
     4681 tagaaatcga agcgaaagcg gcgctgtcgg cgagtgttaa cgtagtcggt tcttatactt
     4741 acaccgatgc ggaatacacc accgatacta cctataaagg caatacgcct gcacaggtgc
     4801 caaaacacat ggcttcgctg tgggctgact ataccttctt tgacggtccg ctttcaggtc
     4861 tgacgctggg caccggtggt cgttatactg gctccagcta tggtgatccg gctaactcct
     4921 ttaaagtggg aagttatacg gtcgtggatg cgttagtgcg ttatgatctg gcgcgagtcg
     4981 gcatggcggg ctccaacgtg gcgctgcatg ttaacaacct gttcgatcgt gaatacgtcg
     5041 ccagctgctt taacacttat ggctgcttct ggggcgcaga acgtcaggtc gttgcaaccg
     5101 caacctttcg tttctaattt ctcttttggg gcacggattt ccgtgcccat ttcacaagtt
     5161 ggctgttatg caggaataca cgaatcattc cgataccact tttgcactgc gtaatatctc
     5221 ctttcgtgtg cccgggcgca cgcttttgca tccgctgtcg ttaacctttc ctgccgggaa
     5281 agtgaccggt ctgattggtc acaacggttc tggtaaatcc actctgctca aaatgcttgg
     5341 tcgtcatcag cctccgtcag aaggggagat tcttcttgat gcccagccgc tggaaagctg
     5401 gagcagcaaa gcgtttgccc gcaaagtggc ttatttgccg cagcagcttc ctccggcaga
     5461 agggatgacc gtgcgtgaac tggtggcgat tggtcgttac ccgtggcatg gcgcgctggg
     5521 gcgctttggt gcggcagatc gcgaaaaggt cgaggaagct atctcgctgg ttggcttaaa
     5581 accgctggcg catcggctgg tcgatagtct ctctggcggc gaacgtcagc gggcgtggat
     5641 cgccatgctg gtggcgcagg atagccgttg tctgttgctc gacgaaccga cttcagcgct
     5701 ggatatcgcc caccaggttg atgtgctggc gctggtgcac cgtttaagtc aggaacgtgg
     5761 cctgacggtc attgccgtgt tgcacgatat caatatggcg gcacgctact gtgattatct
     5821 ggtcgccctg cgcagtggtg aaatgattgc tcagggaacg cctgcggaaa ttatgcgcgg
     5881 cgaaaccctc gaaatgattt atggcatccc gatgggtatt ttgccgcatc cggcgggtgc
     5941 tgcacctgtg agttttgttt attgatgagc ggcttacctc ttatttcgcg ccgtcgactg
     6001 ttaacggcga tggcgctttc tccgttgtta tggcagatga ataccgccca cgcggcggct
     6061 attgacccca atcgtattgt ggcgctggag tggttgccgg tggaattact gctggcgctc
     6121 ggcatcgtgc cttacggcgt ggcggatacc atcaactatc gcctgtgggt cagcgaacca
     6181 ccattgccgg actcagtgat tgacgtcggt ttgcgtacag aacctaacct tgaactgatg
     6241 accgaaatga aaccgtcgtt tatggtctgg tcggcaggat atggcccttc atcagaaatg
     6301 ctggcacgta ttgcgccggg tcgcggattt aacttcagtg acggcaaaca tccgttggcg
     6361 atggcgcgta aatcgctgac ggaaatggca gatttactta acctgcaaag cgcagcggaa
     6421 acgcatttag cgcaatacga agactttatc cgcagcatga aaccccgctt tgtgaagcgt
     6481 ggtgcacgcc cgttactgct gacgacgctt atcgatccgc gccatatgct ggtcttcggt
     6541 ccaaacagct tgttccagga aattctcgat gagtacggca tcccaaatgc ctggcaaggg
     6601 gaaaccaact tctggggcag taccgccgtc agtatcgatc gtctggcagc gtataaagac
     6661 gttgatgtgc tctgttttga tcacgacaac agcaaagaca tggatgcgct aatggcaacc
     6721 ccgctgtggc aggccatgcc gtttgtccgt acagggcgct ttcagcgcgt acccgcagtc
     6781 tggttttatg gtgcaacgct ttcggcaatg cactttgtgc gtgttctgga taacgccatc
     6841 ggaggtaaag cgtgagtaaa cgaattgcgc ttttcccggc gttattgctg gcgctgttag
     6901 tgattgtcgc tacggcgctc acctggatga acttctcaca ggcgctgccg cgcagtcagt
     6961 gggcgcaggc cgcctggtcg ccggatattg acgtcatcga gcagatgatt tttcactaca
     7021 gcttgttgcc gcgtctggcg atttcgctgc tggtgggcgc gggcctgggg ctggtgggcg
     7081 tgctgtttca gcaagtactg cgtaacccgc tggcggaacc gacgacgttg ggcgttgcaa
     7141 caggcgcgca actggggatt accgtcacca cgctctgggc gatccctagc gcgatggcga
     7201 gccagtttgc tgcgctggca ggggcttgcg ttgttggctt aattgttttt ggcgtcgcgt
     7261 gggggaaacg gctgtcgccg gtaacgctga ttttcgcggg gttggtagtg agcctttatt
     7321 gcggcgcaat caatcagtta ctggttatct tccatcatga ccagttgcaa agcatgtttc
     7381 tgtggagcac tggaacgctg acgcaaaccg actggggcgg tgttgagcgt ttatggccgc
     7441 agttgctggg cggtgtgatg ctgacgttgc tgctacttcg tccgttaacc ctgatggggc
     7501 ttgatgatgg cgtggcgcgc aatctcgggc tggccttgtc gcttgcgcgt ctggcggcgc
     7561 tgtcgctggc gattgtcatc agtgcgctgc tggtgaacgc tgtggggatt atcggcttta
     7621 tcgggttgtt cgcgccgctg ctggcaaaaa tgctgggggc gcggcgtctg ctgccacgac
     7681 tgatgctggc gtcgttgatt ggtgcgctga tcctctggct ttccgatcaa atcatcctct
     7741 ggctgactcg cgtgtggatg gaagtgtcca ccggttcggt cactgcgttg atcggtgcgc
     7801 cgctgctact gtggctgctg ccgcgtttac gcagcattag cgcgccggat atgaaggtta
     7861 acgatcgtgt cgcgactgaa cgccaacatg tgctggcgtt tgccctcgcg ggcggcgtgc
     7921 tgttgttgat ggctgtggtg gtggcgctgt cgtttggtcg tgatgcgcac ggctggacgt
     7981 gggcgagcgg ggcgttgctc gaggatttaa tgccctggcg ctggccgcga attatggcgg
     8041 cgctgtttgc gggcgtcatg ctggcggtag cgggctgtat tattcagcga ctgaccggca
     8101 acccgatggc aagcccggaa gtgctgggga ttagctccgg cgcggcgttt ggtgtggtgt
     8161 taatgctgtt tctggtaccg ggtaatgcct ttggctggtt gttacccgca ggcagtctcg
     8221 gggcggcggt gacgctgttg atcattatga tcgccgccgg acgcggtgga ttttccccac
     8281 accgcatgtt actggcgggg atggcgttaa gtaccgcgtt caccatgctt ttgatgatgt
     8341 tgcaggcaag tggtgacccg cgaatggcgc aagtgctgac ctggatttcc ggttcgacct
     8401 acaacgcgac cgatgctcag gtctggcgca ccggaattgt gatggtgatt ttgctggcga
     8461 ttactccgct gtgccgccgc tggctgacca ttttaccgct gggtggtgat accgcgcggg
     8521 cggtagggat ggcgctgacg ccgacgcgaa ttgcgctgct gctgttagcg gcttgcctga
     8581 cggcgaccgc gacgatgact attggaccgt tgagttttgt tggtttaatg gcaccgcata
     8641 ttgcgcggat gatgggcttt cgacggacga tgccacacat cgtaatttcg gcgctggtgg
     8701 gtggtttact gctggtgttc gctgactggt gtgggcggat ggtgctgttt ccattccaga
     8761 tcccggcggg gctgctgtcg acctttatcg gtgcgccata ttttatctat ttgctgagaa
     8821 agcagagccg ttaatagaat gcctcgcccg gtgggcgagg caaaattatc acaacttcgc
     8881 gaacacccga cgtgcagcat cgatggtgtt attgatatct tccatgctgt gcgccacaga
     8941 cataaagccc gcttcaaacg ctgacggtgc caggtaaaca ccttcatcca gcatcatatg
     9001 gaagaaacgc ttaaaacgct cgacgtcaca ggccatcaca tcctgatagc acgtcacgga
     9061 ctcggcgtcg gtaaagaaga taccgaacat accaccgacg tggttaacga ccagcggaat
     9121 tcccgcttct tctgccgctt ccagcagacc ttctgccaga cgtgttgtca gctcatccag
     9181 cgtttcgtga atgcccggct gcgcgacttc attcagacag gcgaaacccg ctgccatcgc
     9241 aatcgggtta ccggaaagcg tacccgcctg atagaccgga cctgtcgggg ccagcgcatc
     9301 cattacatca cgacgaccac cgaatgcgcc taccggcatt ccaccgccga tgatttttcc
     9361 gaggcaggtt aaatctggca ctacggcgaa ataatcctgt gcgccagcta gcgctacgcg
     9421 gaaaccggtc atcacttcat cgatgatcag caacgcgcca aattcgtcgc acagcgcacg
     9481 cagacctggc aggaactctg gcagcggcgg aacacagttc atattgcctg ccaccggctc
     9541 gacgataata caggcaatct cttgcgggta ttgctcaaac gcggcgcgta cagaggccag
     9601 atcgttataa gtacaggtta aggtatgttt ggcgaaatct gccggaacgc ccggcgagtt
     9661 tggctggcct aacgtgagtg cgccagaacc ggctttcacc agcaggcagt cagcgtgacc
     9721 gtggtaacaa ccttcaaatt taatgatttt gtcgcgaccg gtaaaaccac gggccaggcg
     9781 gatggcgctc atcgtcgcct cggtgccgga gttcaccatg cgcaccatat ccatggtcgg
     9841 gaccagttcg gtcaccagtt gcgccatttt cacttccatt tcggtcggtg caccaaagct
     9901 taaaccacgc tcggcggctt caatcacggc attgcggatc gccggatggt tatggcccag
     9961 caccatcggc ccccaggaac cgacataatc gatataggct ttgccatcaa catcgtacag
    10021 ataagcgccg tccgcttttt cgataaacag tggagtgccg cccacgccag taaaggcgcg
    10081 aacaggggag ttcacaccgc cagggatcag ctcgcgcgct gcgctgtaaa gattttcaga
    10141 cttactcatg gagggttcct gattcgtaga aaaagtgaat ggctgctatt ccatgttatt
    10201 cataacaagt taaatactcg tcaaacatca ggctgcttgt actggtgcaa tcctgatttc
    10261 ttagagtata aaagttttgt gcatttgaaa cattacgctt tgcaaaggat tttcatggaa
    10321 cgtgcgagta aaatgccgtc atcttatttg tatgaccaat aagtgatcat tggatgaaaa
    10381 ctgatactcc ctctttagaa acaccgcagg ccgcgcgcct gcgacgcaga caactgattc
    10441 gccaacttct tgagcgcgat aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg
    10501 gcacgcttgt cgggctggct gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc
    10561 aacgtatggg ggcgctggta catactgctg ataattatcc gctactgtta accgtcgctt
    10621 ttctctgttc ggcggtgctg gcgatgttcg gctacttttt ggtgcgcaaa tacgcgcccg
    10681 aagcaggggg atcggggatc ccggaaattg aaggggcgct ggaagatcaa cgtcctgttc
    10741 gctggtggcg tgtattgccg gtgaaattct ttggcgggct ggggacactc ggcggaggca
    10801 tggggctagg gcgcgaagga ccaaccgtgc agatcggcgg caatatcagt cgcatggtgc
    10861 ttgatatttt ccgcctgaaa ggtgatgaag ctcgccatac gctgctggca accggtgctg
    10921 ctgcggggct ggctgcggcc tttaacgcgc cgctggcggg tattttgttt attatcgaag
    10981 agatgcgtcc gcagtttcgc tatacgttaa tttcgattaa agcggtattt attggcgtca
    11041 ttatgtcgac cattatgtac cggattttta atcatgaagt tgcgttgatt gacgtcggta
    11101 aactttctga tgcgccgctt aatacgctgt ggctttatct gatcctcggt attatttttg
    11161 gcattttcgg ccctattttt aataaatggg tgctggggat gcaggatttg ctgcaccgtg
    11221 tgcacggcgg caatattacc aaatgggtac tgatgggggg tgcgattggc ggtctgtgtg
    11281 gattgctggg gtttgtggca ccagcaacgt cgggcggcgg ttttaacctg attcctatcg
    11341 ctaccgcggg gaatttcagc atgggaatgc tggtgtttat cttcgtcgct cgggtcatta
    11401 ccaccttact ctgcttctct tccggcgcgc cgggcggtat ttttgccccg atgctggcgc
    11461 tgggtactgt gctgggaacc gctttcggaa tggttgccgt tgagctgttt ccgcaatatc
    11521 acctggaggc ggggacgttt gctattgccg gaatgggggc attactggcg gcatctattc
    11581 gcgcgccgtt aacggggatc attctggttc tggagatgac cgataactac cagctcattt
    11641 tgccaatgat tattaccggt cttggcgcaa cactattagc gcaatttacc ggcgggaaac
    11701 ctctatactc ggcgattctt gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc
    11761 gaagcaaggc cgcatcagcc agcgagaata cttgaacgaa ataccagggt attagataat
    11821 ggcgattatt attgggttag aatttgcccg attgccgatg tcgtttggag caaaatatga
    11881 gtgatgacgt agcactgccg ctggagttta ccgacgcagc agccaacaaa gttaaaagcc
    11941 tgatcgctga cgaagataac ccgaatctga aattacgcgt gtatatcacc ggtggcggtt
    12001 gcagcggctt ccagtatggt ttcacctttg atgatcaggt gaacgaaggc gatatgacca
    12061 tcgaaaaaca gggcgttggc ctggtggttg atccgatgag cctgcaatat ctggtcggcg
    12121 gttccgttga ctacaccgaa ggactggaag gttctcgttt catcgtgacc aacccgaacg
    12181 cgaaaagcac ctgcggttgc ggttcttcct ttagcatcta atctgttgtt catgtgccgg
    12241 ataaactatc cggcacatct tctctcttca acgcccattc tcatccagcg caaacgtcgg
    12301 tagcttaaga tgccaacgaa tagccgccag ccgaatcaat agcgtcacga ccatgcccat
    12361 catactggct gtttccagtg gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc
    12421 gccgataata caggcagttg cgtagatttc tgtacgtaaa atcatgggga tttcgcggac
    12481 cagtacatca cgaatgatcc cgccgccaac gccagtaatg acgcccatac aaaccgcgat
    12541 taacggaccg gcttccgcat taaaggcttt attcacgcca atgccgacaa acaccgccag
    12601 accaacggcg tccagcaccg gcaacatcca ttttggtaag cgtcttggct ggcgcaccag
    12661 cacgatggtc agcatgctgg tgaccatggc aacgaccaga tcggtgggat ctttcaccca
    12721 aaataccggg ccgtgatcca gcgccatgtc gcgaattgtt ccgtcgccta ctgcggtaac
    12781 cacgcccagt accagaacac caaaagggtc catacgcaat tttccggcta acaaaacgcc
    12841 gtagatggca aataccgctg tgccgactat atccagccaa tagacgagca ttgttcaatc
    12901 cccactgagc acctgaaaag gtcaggcact aatctacctg tgaaagcgca ttacagagct
    12961 gttgtgctgc gaggataata cgtgggcttg cacgttcaaa ccagtcactc gtgagaggaa
    13021 taacgggaat tttgagctgt tcaccccagt attgtttgat tttaggaatt tggtccggtc
    13081 cgcctgtaat gacaatagcc tgtggcgagc gtgctaacac ctgttcgcgg ctaacttgcg
    13141 gccagggaac ccggctgtct ttaaagatgt tttctccgcc acaaacttcg agtacctggt
    13201 tctgaatcga ctcttttcca ctggtaaatg gcggattaat gccgaattgc agaaaaacac
    13261 gttttttagg tttatcagca tattgcgctt tcaattgcac gtactgatcc agcagggatt
    13321 gcgcggcttg ttcggccttg tctggttgcg gactccaggg ggccagttga cgtaacgcat
    13381 tggcaatttg ttcaatgctt gtcgcatcga cccacatcac ttttattccc agcgaagcca
    13441 gctggtcaac ctgccgctcg gcattacctc cacgccaggc aatcaccaga tcgggtttca
    13501 gcgcgacaat gcgttccaga ttcatcccct gccaggtgga aacctgctca atcttttgcg
    13561 cttgtggagg atagtcggaa tagctgctga ccccaaccgg cgtgatcccg gcggcaaagg
    13621 caagttcagt gttggcggga gaaagcgtga tgacgcgcgg cgcggcgttg agccacagtg
    13681 gcgcgagaaa agacagggcg accagcgccc tgaacagtga tttagccatg tgccagtttc
    13741 tgcaccagtg actcaaccat caggctggac tgtttagcgg caacagccag gaactcatcg
    13801 aagctaagat gagactgttg atcggccacg tcggagatgg cgcgtacgac aacaaacggg
    13861 acgttgaaat tgtggcagac atgggcgatt gccgtcgctt ccatctctac agcaatggcc
    13921 tgtgggaagt tgtggcggat tttcgccaga ccaacagaac cgttgatgaa agcgtcgccg
    13981 ctaacaatca ggccacgtac agcgttaaga ttcagttcgg caatgcaggc ctcagcggca
    14041 gcgatcagtt tatcgtcagc tttaaagcct gccggacagc ctggtaactg accgtattca
    14101 taaccaaatg ccgtgacatc cgcgtcgtga taacgcgctt cgtccgagac aacgatatcg
    14161 cccactttca gcgttggtgc caggccaccg gcagaaccgg tgttaataat cacatctggc
    14221 ttgcagtgtt ccaacagcag agtggcaccc agcgccgcag cgactttacc gatgcccgat
    14281 ttcagaagcg caacctcggt tccattcagt tggccggtat agatttcgca accgccgaga
    14341 ctgatagttt gacggttttc gattttgtca cgcagcagcg taacttcttc ttccattgca
    14401 ccaatgatgc cgattttcat agatttactc gcgataagcc cgatttgaag gcatagttta
    14461 ccatgcgctt acggggaagc gtatttctca cgcgggagag gacatggcac agattgattt
    14521 ccgaaaaaaa ataaactggc atcgtcgtta ccgttcaccg cagggcgtta aaaccgaaca
    14581 tgagatcctg cggatcttcg agagcgatcg cgggcgtatc atcaactctc cggcaattcg
    14641 tcgtctgcaa caaaagaccc aggtttttcc actggagcgc aatgccgccg tgcgcacgcg
    14701 tcttacccac tcgatggaag tccagcaggt gggacgctac atcgccaaag aaattttaag
    14761 ccgtctgaaa gagcttaaat tgctggaagc atacggcctg gatgaactga ccggcccctt
    14821 tgaaagcatt gttgagatgt catgcctgat gcacgatatc ggcaatccgc cgtttggtca
    14881 ttttggcgaa gcagcgataa atgactggtt tcgccaacgt ttgcacccgg aagatgccga
    14941 aagccagcct ctgactgacg atcgctgcag cgtggcggca ctacgtttac gggacgggga
    15001 agaaccgctt aacgagctgc ggcgcaagat tcgtcaggac ttatgtcatt ttgaggggaa
    15061 tgcacaaggc attcgtctgg tgcatacatt gatgcggatg aatctcactt gggcacaggt
    15121 tggcggtatt ttaaaatata cccgtccggc gtggtggcgt ggcgaaacgc ctgagacaca
    15181 tcactattta atgaaaaagc cgggttatta tctttctgaa gaagcctata ttgcccgctt
    15241 gcgtaaagaa cttaatttgg cgctttacag tcgttttcca ttaacgtgga ttatggaagc
    15301 tgccgacgac atctcctatt gtgtggcaga ccttgaagat gcggtagaga aaagaatatt
    15361 taccgttgag cagctttatc atcatttgca cgaagcgtgg ggccagcatg agaaaggttc
    15421 gctcttttcg ctggtggttg aaaatgcctg ggaaaaatca cgctcaaata gtttaagccg
    15481 cagtacggaa gatcagtttt ttatgtattt acgggtaaac accctaaata aactggtacc
    15541 ctacgcggca caacgattta ttgataatct gcctgcgatt ttcgccggaa cgtttaatca
    15601 tgcattattg gaagatgcca gcgaatgcag cgatcttctt aagctatata aaaatgtcgc
    15661 tgtaaaacat gtgtttagcc atccggatgt cgagcagctt gaattgcagg gctatcgggt
    15721 cattagcgga ttattagaga tttatcgtcc tttattaagc ctgtcgttat cagactttac
    15781 tgaactggta gaaaaagaac gggtgaaacg tttccctatt gagtcgcgct tattccacaa
    15841 actctcgacg cgccatcggc tggcctatgt cgaggctgtc agtaaattac cgtcagattc
    15901 tcctgagttt ccgctatggg aatattatta ccgttgccgc ctgctgcagg attatatcag
    15961 cggtatgacc gacctctatg cgtgggatga ataccgacgt ctgatggccg tagaacaata
    16021 accaggcttt tgtaaagacg aacaataaat ttttaccttt tgcagaaact ttagttcgga
    16081 acttcgggct ataaaacgaa tctgaagaac acagcaattt tgcgttatct gttaatcgag
    16141 actgaaatac atgaaaaaaa ccacattagc actgagtgca ctggctctga gtttaggttt
    16201 ggcgttatct ccgctctctg caacggcggc tgagacttct tcagcaacga cagcccagca
    16261 gatgccaagc cttgcaccga tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt
    16321 agaaggtagc acaaccgtta atacgccgcg tatgccgcgt aatttccagc agttcttcgg
    16381 tgatgattct ccgttctgcc aggaaggttc tccgttccag agctctccgt tctgccaggg
    16441 gggccagggc ggtaatggcg gcggccagca acagaaattc atggcgctgg gttccggcgt
    16501 catcattgat gccgataaag gctatgtcgt caccaacaac cacgttgttg ataacgcgac
    16561 ggtgattaaa gtccagctga gcgatggtcg taagttcgac gcgaagatgg ttggcaaaga
    16621 tccgcgctct gatatcgcgc tgatccaaat ccagaacccg aaaaaactga ccgcaattaa
    16681 gatggcggat tctgatgcac tgcgcgtggg tgattacacc gtagcgattg gtaacccgtt
    16741 tggtctgggc gagacggtaa cttccgggat tgtctctgcg ctggggcgta gcggcctgaa
    16801 tgctgaaaac tacgaaaact tcatccagac cgatgcagcg atcaaccgtg gtaactccgg
    16861 tggtgcgctg gttaacctga acggcgaact gatcggtatc aacaccgcga tcctcgcacc
    16921 ggacggcggc aacatcggta tcggttttgc tatcccgagt aacatggtga aaaacctgac
    16981 ctcgcagatg gtggaatacg gccaggtgaa acgcggtgag ctgggtatta tggggactga
    17041 gctgaattcc gaactggcga aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag
    17101 ccaggttctg cctaattcct ccgctgcaaa agcgggcatt aaagcgggtg atgtgatcac
    17161 ctcactgaac ggtaagccga tcagcagctt tgccgcactg cgtgctcagg tgggtactat
    17221 gccggtgggc agcaaactga ccctgggctt actgcgcgac ggtaagcagg ttaacgtgaa
    17281 cctggaactg cagcagagca gccagaatca ggttgattcc agctccatct tcaacggcat
    17341 tgaaggcgct gagatgagca acaaaggcaa agatcagggc gtggtagtga acaacgtgaa
    17401 gacgggcact ccggctgcgc agatcggcct gaagaaaggt gatgtgatta ttggtgcgaa
    17461 ccagcaggca gtgaaaaaca tcgctgaact gcgtaaagtt ctcgacagca aaccgtctgt
    17521 gctggcactg aacattcagc gcggcgacag caccatctat ctgttaatgc agtaatctcc
    17581 ctcaacccct tcctgaatac gggaaggggt tctccttaca atctgtgaac ttcgccacaa
    17641 ctccatacat cttcatcatc ctttgggcat ttgcacaatg ccgtacgtta cgtacttcct
    17701 tatgttaagc cgtgcataac ggaggactta tggctggctg gcatcttgat accaaaatgg
    17761 cgcaggatat cgtggcacgt accatgcgca tcatcgatac caatatcaac gtaatggatg
    17821 cccgcgggcg aattatcggc agcggcgatc gtgagcgtat tggtgaattg cacgaaggtg
    17881 cattgctggt actttcacag ggacgagtcg tcgatatcga tgacgcggta gcacgtcatc
    17941 tgcacggtgt gcggcagggg attaatctac cgttacggct ggaaggtgaa attgtcggcg
    18001 taattggcct gacaggtgaa ccagagaatc tgcgtaaata tggcgaactg gtctgcatga
    18061 cggctgaaat gatgctggaa cagtcgcggt tgatgcactt gttggcgcag gatagccgtt
    18121 tgcgggaaga actggtgatg aacctgattc aggcagagga gaatactccc gcacttctga
    18181 atgggcgcaa cggctgggga tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt
    18241 cgacagcggt cagcttggcg tggacagcgc aatggcggag ttacaacaac tgcaaaacgc
    18301 gctgactacg cccgagcgta ataatctggt ggcgattgtc tcgctaaccg aaatggtggt
    18361 gttgaaaccg gcgttgaact cttttgggcg ctgggatgca gaagatcatc gtaagcgagt
    18421 tgaacaactg attacccgca tgaaagagta cggccagctg cgttttcgcg tttcactggg
    18481 caactatttt accggtcctg gcagtattgc ccgatcctat cgtacggcga aaacgacgat
    18541 ggtggtgggt aaacagcgga tgccagaaag tcgctgctat ttttatcagg atctgatgtt
    18601 acctgtgtta ctcgacagtt tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct
    18661 ggcgcggctg aaagcgatgg acaataacgg cttgctgcga cgaacgctgg cggcgtggtt
    18721 tcgccacaat gtgcaaccgc tggcaacgtc aaaggcgttg tttattcatc gtaataccct
    18781 ggagtatcgg cttaatcgta tatcggaact gaccgggctt gatttgggca attttgatga
    18841 caggttgctg ctgtatgtgg cgttacaact ggatgaagag cggtagatta tgcgttaagg
    18901 ttgaggcggc gctggctcat tcccctcagc ccgaagggga gaggggaatg cccgagttaa
    18961 ccgtcaattc gggattattt acgcgttagt ttttccagat cggcttcaat ctcgctgatc
    19021 ttattggtta cgactgactc caggtgacgt aagtcgtcga ggatcttacg cttaagatca
    19081 acttcgctgc ggtcacgctg gcagatttga tcaagctcat caatgatata ccgtagattc
    19141 gggctgattt cctggacttc tttataaccc tgacccacac catcagcgac gaccgtttta
    19201 cgctgacgcg gatatttaaa cttaacgctc ttggcgaaaa actcgccttt gtctttctgg
    19261 aaatagattt tcagaatatc gttgttggct tcctgccgga ggctgtaacg atcaatttct
    19321 tcaggattgg taatacccag acttttcaga ttgtcgtaca tagcgttacc tcaaaatgag
    19381 tcagtaaatt gtgcttattt tagcatttgg cttggcccgc ccggctcgac tgtgatcgca
    19441 gaaagccttt ttcttccaga cttctatcat ggcgtaaaaa tcaaaaaatt acctgcttta
    19501 ttgtggcgat attccatggg atggattagc acatttatct tcgttttgcg aattacatga
    19561 cagtccgaaa caagatgaaa aagtatttaa aaaaggggga ggaagaatga gtaggacgaa
    19621 ttattctgag ccagaaagca tacgggcagc atcatgctgc ccgtatatgt actcttaatc
    19681 gatggtacgc agcagttcgt taatgccgac tttgccgcga gttttcgcgt caactttctt
    19741 aacgataacc gcacagtaga ggctgtattt gccatctttt gacggcagat tacctgaaac
    19801 aaccacagac cccgccggaa cgcgaccgta gtggatttcg ccggtttcac ggtcgtaaat
    19861 acgggtgctc tgaccaatgt atacgcccat agagatgacg gaaccttctt cgacaatcac
    19921 cccttcaacc acttcagagc gtgcgccaat gaagcagtta tcttcaataa tggtcgggtt
    19981 agcctgcagc ggctccagca cgccgccgat gcccacgcca ccggaaaggt ggacgttttt
    20041 accaatctgc gcacaagaac cgacggtcgc ccaggtatca accatggtgc cttcatcaac
    20101 atatgcgccg atgttaacgt aagacggcat cagcacggtg ttacgggcaa taaacgcacc
    20161 ctgacgtacc gccgctggtg gcacaacgcg gaagccttct ttctggaaac gtgcttcgtc
    20221 gtagtcggcg aatttcatcg gcactttgtc gaagtagcgg ctttctgccc cttcgatcac
    20281 ctgattatca ttaatacgga aagagagcag caccgctttt ttcaaccact gatgcgtcac
    20341 ccactgaccg tcaatttttt ccgctacacg cagtgcgccg gaatccagca gggcgatcac
    20401 ctgatttacc gcttcgcggg taacggtgtc tgcattggct ggcgtgatct cggcacggcg
    20461 ttcaaaagcg gtttcaataa tgttctgtaa ctgctgcatt gttaaactct tttcatatca
    20521 gtaaacacat caccctttat cgtttggatt gagggcctct gtcaaccgct gatgcacttc
    20581 ctgctgcaac tcgttattaa gcgcacgccg gtcagcggtg gcaattatga ataaatcttc
    20641 tactcgctcg ccaatggttg taattcgggc accatgaagc gaaattccca gatcggcaaa
    20701 aattttcccg actcgcgcca gcagtccagg ttggtcgagg gcgatcagtt cgaggaacga
    20761 tttgcggtcg gtatgggtcg gcaaaaacgt tacttcggtt tcaacagtaa aatggcgtaa
    20821 tttggcgggt tggcgacggg gctgcggtgg ctgccagcta ctttgcgtca gcacctgctc
    20881 cagaccaaac cgaataacct catggcgatc tgcggacagc gggctgccat cgggttccag
    20941 cacgataaag gtatccatcg ccataccgtc gcgagtggtg aaaatttgtg cgtcgtgaac
    21001 acttaaattg cggcggtcta attcggcaca gacggcggca aacagatagg ggcggtccgg
    21061 gctccagata aaaatctcgg tgcctccacg cgtagcctgc gggctaagca ataccagcgg
    21121 tttgcttaaa tcatgctgta ataaatggcg ggcatgccag gccagttgat ttgggctatg
    21181 gcggacaaaa tagttagcac gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat
    21241 gttatccatg cgcagtagtg ccagtgcctg gagttggtga tggcgaaccc gttcgcgcat
    21301 atccggcgtg ttttgcattc cgcgtcgtag ctgcttttcg gtggcaaaat agagttcgcg
    21361 caacagactt tgcttccagc tattccacag tgtttcgttg gtggcgcaaa tgtcagccac
    21421 tgtcaggcat accagatagc gcagacgatt ttccgtttga acttcttcgg caaactgctt
    21481 gatgacttca gggtcctgaa tatcgcggcg ttgtgctgtc accgacatca gcaagtgctg
    21541 gtgaaccagc caggcaacca gctgtgtttc gcgtgagttc agcccgtgga gttctgcaaa
    21601 atgcactaca tcctgagcac cgagaatgga gtggtcgccg ccgcgtcctt tggcgatatc
    21661 gtgaaacagc gcggcgatga aaatcagctc agttgacggc aggcgcggcc agacgtccac
    21721 acacaacgga tggcgctggc gcgtttcttc actggcaaaa ctctccagtt tcagcatcac
    21781 gcggatagta tgttcatcca ccgtgtaggc gtggaacaga tcaaactgca tctgcccgac
    21841 gatatgcgac cattgcggca tatacgcgcc gagcacgcta tggcgatgca ttggcaatag
    21901 tccgcgccgc accgctccgg ggtgacgcag aatgctcaaa aacagttttc gtgcttgcgg
    21961 aatattacac agcggttgtt gcagatggcg acgggcatgg cgtaactggc gcagcgtggt
    22021 ggagtaaatg ccggtgatcg cactgttgcg caccatggtg tagaacatac gcaagatggc
    22081 ttccggctgg cgcataaata gtgtttcatc acgcaggtcg attagcgtac cgcgtagctg
    22141 aaactcatcg tcgagtggac gtggtttttc gtcggcggga agggcgagga tggcttcatc
    22201 gaacagttgc agcagcatct ggttgagttc actgacgcgg cgtgtaacgc ggaagtaatc
    22261 cttcatcatc cgctcgaccg gttcgttgcc ttcaccactg taattcagac gctgagcgac
    22321 gctaagttgg cgatcgaata acagacgatt atcgtaacgg ctgacgacca gatgcagggc
    22381 aaagcgaata cgccacagta tatgcagaca ttcgtttaat tccgcccgct ccgctgaggt
    22441 taagaagcca aacccgacca tttcatccag cgatgttgcg ccaaaatgac ggcgggccac
    22501 ccattgcaga gtgtgaatat cgcgcaagcc gccagggcta cttttgatgt ctggttcaag
    22561 gttgtagctg gtgccatggt aacgctgatg gcgctggttc tgttcttcaa ctttcgctgc
    22621 gtagaacttg tcggaaggcc agaatccttc gctaaaaata tgtttttgca gttcgaggaa
    22681 gagcgcaaca tcgccaatta ataagcggga ttcgattaaa ttggtggcga cggttaaatc
    22741 cgataaccct tccaacatgc actcttcaag cgtgcgcaca ctatgaccga cttccagctt
    22801 tacatcccag agcagcgtta acagctcgcc cactttttgc gcctgatcgt ccgggagctt
    22861 tttacggctt aaaatcagta aatcgatgtc agaaagtgga tgcagctcgc cacgaccgta
    22921 gccaccgacg gcaaccaatg ccaggtcggc aatctggctg aatcccgctt caatccataa
    22981 tcgttgcagg agctggtcta tgaactcggt acgtgcctca atcaactgtt ctgcagagat
    23041 cccattgtca aaggcatcac ccagccaacg ctggaaagta tcgatatggg cttttatccc
    23101 acagacggtt aattcatcac ggggccaggc gcatggattt tgcggttgac cgggcagggt
    23161 ggagagagcg gtgtttgcgt actgttctgg aagggtattc attgtgcgcc acccataaga
    23221 ttaaattatc gcattaaaaa agccggcatt cgccggcttc atcttattcg tcgtgcgaga
    23281 ttatcgccgg gatggtgtca tccttgcgta gcgtcaaaat ttcgcagccg ttatcagtca
    23341 ccacaatagt atgctcatat tgtgcagaca agctgcgatc tttggttttt accgtccagc
    23401 catctttcat ggtgcggatc tcttttttac ccgcgttgac cattggctca atggtgaacg
    23461 tcatccccgg tttcagcacg acgttggttt cacgggagtc atagtgcagc acctgcggtt
    23521 cttcatggaa gccgcgacca ataccgtgtc cgcaatattc acgaacgacg gagaagcctt
    23581 ctgcttcgac aaatttctga atcgccgcac cgatttcgcg cagattaatg cctggtttta
    23641 ccatgcgtag cgccaggtac aggctttctt gcgtgatgcg gcacaggcgt tcgcccatga
    23701 tggtcggctt accgacgata aacattttcg aggtatcgcc gtggaaacca tctttgatta
    23761 cggtgacatc aatgttaacg atatcgccat ctttcagcag cttagcatcg tccgggatac
    23821 cgtggcacac cacttcatta atagagatgc aaacggattt cggatagccg tgatagccga
    23881 ggcaggcaga aaccgcgtgt tgttcattaa caatgtaatc attacagatg cgatccagct
    23941 cgccggtgct gacgcccggt ttaacatacg gttcgatcat ctccagcact tcggcagcca
    24001 gtcggccagc gacgcgcatt ttttcgatat cttctggggt cttgattgag atagccatta
    24061 attctgtcca tcagcgtcgg tgataccgac aatatatatg taagtgccgt caatggtatc
    24121 acacccgggc aaattgagaa tcattctgaa tttcgccaaa cgtgccactg aaggttttct
    24181 ataatagaaa attcgacgtc tgatgctgta cacagcgcca acaattattg gtgtccacga
    24241 cgtatttgtg gtataaagcg cgccggactt ccgatccatt tcgtatacac agactggacg
    24301 gaagcgacaa tctcactttg tgtaacaaca cacacgtatc ggcacatatt ccggggtgcc
    24361 ctttggggtc ggtaatatgg gatacgtgga ggcataaccc caacttttat atagaggttt
    24421 taatcatggc aactgtttcc atgcgcgaca tgctcaaggc tggtgttcac ttcggtcacc
    24481 agacccgtta ctggaacccg aaaatgaagc cgttcatctt cggtgcgcgt aacaaagttc
    24541 acatcatcaa ccttgagaaa actgtaccga tgttcaacga agctctggct gaactgaaca
    24601 agattgtttc tcgcaaaggt aaaatccttt tcgttggtac taaacgcgct gcaagcgaag
    24661 cggtgaaaga cgctgctctg agctgcgacc agttcttcgt gaaccatcgc tggctgggcg
    24721 gtatgctgac taactggaaa accgttcgtc agtccatcaa acgtctgaaa gacctggaaa
    24781 ctcagtctca ggacggtact ttcgacaagc tgaccaagaa agaagcgctg atgcgcactc
    24841 gtgagctgga gaaactggaa aacagcctgg gcggtatcaa agacatgggc ggtctgccgg
    24901 acgctctgtt tgtaatcgat gctgaccacg aacacattgc tatcaaagaa gcaaacaact
    24961 tgggtattcc ggtatttgct atcgttgata ccaactctga tccggacggt gttgacttcg
    25021 ttatcccggg taacgacgac gcaatccgtg ctgtgaccct gtacctgggc gctgttgctg
    25081 caaccgtacg tgaaggccgt tctcaggatc tggcttccca ggcggaagaa agcttcgtag
    25141 aagctgagta ataaggcttg ataactcccc caaaatagtt cgagttgcag aaaggcggca
    25201 agctcgagaa ttcccgggag cttacatcag taagtgaccg ggatgagcga gcgaagacaa
    25261 cgcatctgcg gcgcgaaata tgaaggggga gagcccttat agaccaggta gtacacgttt
    25321 ggttaggggg cctgcatatg gccccctttt tcacttttat atctgtgcgg tttaatgccg
    25381 ggcagatcac atctccgagg attttagaat ggctgaaatt accgcatccc tggtaaaaga
    25441 gctgcgtgag cgtactggcg caggcatgat ggattgcaaa aaagcactga ctgaagctaa
    25501 cggcgacatc gagctggcaa tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa
    25561 aaaagcaggc aacgttgctg ctgacggcgt gatcaaaacc aaaatcgacg gcaactacgg
    25621 catcattctg gaagttaact gccagactga cttcgttgca aaagacgctg gtttccaggc
    25681 gttcgcagac aaagttctgg acgcagctgt tgctggcaaa atcactgacg ttgaagttct
    25741 gaaagcacag ttcgaagaag aacgtgttgc gctggtagcg aaaattggtg aaaacatcaa
    25801 cattcgccgc gttgctgcgc tggaaggcga cgttctgggt tcttatcagc acggtgcgcg
    25861 tatcggcgtt ctggttgctg ctaaaggcgc tgacgaagag ctggttaaac acatcgctat
    25921 gcacgttgct gcaagcaagc cagaattcat caaaccagaa gacgtatccg ctgaagtggt
    25981 agaaaaagaa taccaggtac agctggatat cgcgatgcag tctggtaagc cgaaagaaat
    26041 cgcagagaaa atggttgaag gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg
    26101 tcagccgttc gttatggaac caagcaaaac tgttggtcag ctgctgaaag agcataacgc
    26161 tgaagtgact ggcttcatcc gtttcgaagt gggtgaaggc atcgagaaag ttgagactga
    26221 ctttgcagca gaagttgctg cgatgtccaa gcagtcttaa ttatcaaaaa ggagccgcct
    26281 gagggcggct tctttttgtg cccatcttgt aaattcagct aacccttgtg gggctgcgct
    26341 gaaaagcgac gtacaatgtc gctagtatta attcatttca atcgttgaca gtctcaggaa
    26401 agaaacatgg ctaccaatgc aaaacccgtc tataaacgca ttctgcttaa gttgagtggc
    26461 gaagctctgc agggcactga aggcttcggt attgatgcaa gcatactgga tcgtatggct
    26521 caggaaatca aagaactggt tgaactgggt attcaggttg gtgtggtgat tggtgggggt
    26581 aacctgttcc gtggcgctgg tctggcgaaa gcgggtatga accgcgttgt gggcgaccac
    26641 atggggatgc tggcgaccgt aatgaacggc ttggcaatgc gtgatgcact gcaccgcgcc
    26701 tatgtgaacg ctcgtctgat gtccgctatt ccattgaatg gcgtgtgcga tagctacagc
    26761 tgggcagaag ctatcagcct gttgcgcaac aaccgtgtgg tgatcctctc cgccggtaca
    26821 ggtaacccgt tctttaccac cgactcagca gcttgcctgc gtggtatcga aattgaagcc
    26881 gatgtggtgc tgaaagcaac caaagttgac ggcgtgttta ccgctgatcc ggcgaaagat
    26941 ccaaccgcaa ccatgtacga gcaactgact tacagcgaag tgctggaaaa agagctgaaa
    27001 gtcatggacc tggcggcctt cacgctggct cgtgaccata aattaccgat tcgtgttttc
    27061 aatatgaaca aaccgggtgc gctgcgccgt gtggtaatgg gtgaaaaaga agggacttta
    27121 atcacggaat aattcccgtg atggataaat aagggtaaga ttccgcgtaa gtatcgcggg
    27181 ggcgtaagtc tggttataag gcgttattgt tgcaggcagt ttggtcacgg ccagcgcgca
    27241 gcaaccggag cgtacaaaag tacgtgagga tggcgagcac tgctcggggc caaaatggca
    27301 aataaaatag cctaataatc cagacgatta cccgtaatat gtttaatcag ggctatactt
    27361 agcacacttc cactgtgtgt gactgtctgg tctgactgag acaagttttc aaggattcgt
    27421 aacgtgatta gcgatatcag aaaagatgct gaagtacgca tggacaaatg cgtagaagcg
    27481 ttcaaaaccc aaatcagcaa aatacgcacg ggtcgtgctt ctcccagcct gctggatggc
    27541 attgtcgtgg aatattacgg cacgccgacg ccgctgcgtc agctggcaag cgtaacggta
    27601 gaagattccc gtactctgaa aatcaacgtg tttgatcgtt caatgtctcc ggccgttgaa
    27661 aaagcgatta tggcctccga tctcggcctg aacccgaact ctgcgggtag cgacatccgt
    27721 gttccgctgc cgccgctgac ggaagaacgt cgtaaagatc tgaccaaaat cgttcgtggt
    27781 gaagcagaac aagcgcgtgt tgcagtacgt aatgtgcgtc gtgacgcgaa cgacaaagtg
    27841 aaagcactgt tgaaagataa agagatcagc gaagacgacg atcgccgttc tcaggacgat
    27901 gtacagaaac tgactgatgc tgcaatcaag aaaattgaag cggcgctggc agacaaagaa
    27961 gcagaactga tgcagttctg atttcttgaa cgacaaaaac gccgctcagt agatccttgc
    28021 ggatcggctg gcggcgtttt gctttttatt ctgtctcaac tctggatgtt tcatgaagca
    28081 actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg tggtgcgcca
    28141 taatcccgaa cacttccgcg tagttgcgct ggtggcaggc aaaaatgtca ctcgcatggt
    28201 agaacagtgc ctggaattct ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa
    28261 acttcttaaa acgatgctac agcaacaggg gagccgcacc gaagtcttaa gtgggcaaca
    28321 agccgcttgc gatatggcag cgcttgagga ggttgatcag gtgatggcag ccattgttgg
    28381 tgctgctggg ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc
    28441 caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa agcagagcaa
    28501 agcgcaattg ttaccggtcg atagcgaaca taacgctatt tttcagagtt taccgcaacc
    28561 tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt ccattttact
    28621 taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa caatgacgcc
    28681 cgatcaagct tgccgtcatc cgaactggtc gatgggacgt aaaatttctg tcgattcggc
    28741 taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta acgccagcgc
    28801 cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg tgcgctatca
    28861 ggacggcagt gttctggcgc agctggggga accggatatg cgtacgccaa ttgcccacac
    28921 catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg ctcgattttt gcaaactaag
    28981 tgcgttgaca tttgccgcac cggattatga gcgttatcca tgcctgaaac tggcgatgga
    29041 ggcgttcgaa caaggccagg cagcgacgac agcattgaat gccgcaaacg aaatcaccgt
    29101 tgctgctttt cttgcgcaac aaatccgctt tacggatatc gctgcgttga atttatccgt
    29161 actggaaaaa atggatatgc gcgaaccaca atgtgtggac gatgtgttat ctgttgatgc
    29221 gaacgcgagt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgag gataatccgg
    29281 ctacagagag tcgcgctatt tgttagcgta gggcttcagt gatatagtct gcgccatctg
    29341 atcgtaagta gttggcttta taaggtcagg tatgccgtgg ttttacacgg cttttttttg
    29401 tataggcttc agtattcctg agtaccgtaa accctgtcag ggaataaaaa acgcgtgatg
    29461 ttgtctgcta ctcaaccact tagcgaaaaa ttgccagcgc atggctgccg tcatgttgcg
    29521 atcattatgg acggcaatgg tcgctgggca aaaaagcaag ggaagattcg tgcctttggg
    29581 cataaagccg gggcaaaatc cgtccgccgg gctgtctctt ttgcggccaa caacggtatt
    29641 gaggcgttaa cgctgtatgc ctttagtagt gaaaactgga accgaccagc gcaggaagtc
    29701 agtgcgttaa tggaactgtt tgtgtgggcg ctcgatagcg aagtaaaaag tctgcaccga
    29761 cataacgtgc gtctgcgtat tattggcgat accagtcgtt ttaactcgcg tttgcaagaa
    29821 cgtattcgta aatctgaagc gctaacagcc ggaaataccg gtctgacgct gaatattgcg
    29881 gcgaactacg gtggacgttg ggatatagtc cagggagtca ggcaactggc tgaaaaggtg
    29941 cagcaaggaa acctgcaacc agatcagata gatgaagaga tgttaaacca gcatgtctgt
    30001 atgcatgaac tggcccctgt agatttagta attaggactg ggggagagca tcgcattagt
    30061 aactttttgc tttggcaaat tgcctatgcc gaactttact ttacagatgt tctctggccc
    30121 gatttcgatg aacaagactt tgaaggggcg ttaaatgcct ttgctaatcg agagcgtcgt
    30181 ttcggcggca ccgagcccgg tgatgaaaca gcctgatggg ggtcgctttt gctgaagtat
    30241 cgcctgatat ctgcttttgt gttaataccc gtcgtcatcg cggcgttgtt tctgttgccg
    30301 ccggtggggt tcgccattgt aacgctggtg gtctgcatgc tggcagcgtg ggaatgggga
    30361 cagcttagcg gttttaccac tcgttcgcag cgagtatggt tggcggtgtt atgcgggtta
    30421 ttgttggcgc tgatgctttt tctgttgccg gaatatcacc gaaatattca tcaaccgctg
    30481 gttgaaatct cactttgggc ttcgttgggt tggtggattg tcgcgctatt gctggtgctg
    30541 ttttacccag gttccgcagc aatctggcgt aactctaaaa cattgcgcct tatttttggc
    30601 gtgctaacca ttgttccctt cttctggggc atgctggcgt tacgggcctg gcactatgac
    30661 gagaatcatt acagtggcgc aatatggctg ctctatgtca tgatcctggt atggggcgct
    30721 gactccggcg catatatgtt tggcaaattg tttggtaaac ataagctggc accgaaggtt
    30781 tctccgggta aaacctggca aggctttata ggtggactcg ctactgcagc ggtaatctca
    30841 tggggttatg gcatgtgggc gaatctcgac gtcgctcccg tcaccttact catttgctct
    30901 attgtcgcag cgttagcctc agtgctcggc gatctgaccg agagtatgtt taagcgtgaa
    30961 gcaggaatta aggacagcgg tcatttaatt ccaggacacg gtggtatttt agatcgtatt
    31021 gatagcctga cggctgcggt accggtcttt gcttgcttgt tgttactggt attcaggacg
    31081 ctttaacgga aggtaatatg ctgagttttc tctgggattt ggcttcgttc atcgttgcac
    31141 tgggtgtact tatcaccgtg catgaatttg gtcatttctg ggttgcccgg cgttgtggtg
    31201 ttcgcgttga gcgtttctca atagggtttg gtaaggcgct ctggcggcga actgataagc
    31261 tcggcaccga atatgttatc gccctgatcc cgttgggcgg ttatgtcaaa atgctggatg
    31321 agcgcgcaga accggtcgtt ccggaactcc gccaccatgc cttcaataat aaatctgtcg
    31381 gccaacgagc ggcgattatt gccgcaggtc cggttgcaaa cttcattttt gctatctttg
    31441 cctactggct ggtttttatt attggtgtgc ctggcgtacg tccggtggtt ggcgaaatag
    31501 cagccaattc gatagctgcg gaagcacaaa ttgcaccagg tacggaacta aaagccgtag
    31561 atggtatcga aacgcctgat tgggatgccg tgcgtttgca gttggtcgat aaaattggcg
    31621 atgaaagcac caccattaca gtagcgccat ttggcagcga ccaacggcgg gatgtaaagc
    31681 tcgatttacg tcactgggcg tttgagcctg ataaagaaga tccggtatct tcgctgggga
    31741 ttcgtcctcg tgggccgcaa attgaacctg tactggaaaa tgtgcagcca aactcggcgg
    31801 caagcaaggc aggtttgcaa gcaggcgaca ggatcgttaa agtcgatggt cagcccttaa
    31861 cgcagtgggt gacctttgtg atgcttgtcc gggataaccc gggtaaatcc ttagcgttag
    31921 aaatcgaaag gcaggggagt cccttgtctt tgacattaat cccggggagt aaaccgggta
    31981 atggtaaagc gattggtttt gtcggtattg agccgaaagt cattcctttg ccagatgagt
    32041 ataaagttgt acgccagtat gggccgttca acgccatcgt cgaagccacg gacaaaacgt
    32101 ggcagctgat gaagctgacg gtcagtatgc tgggaaaatt gatcaccggt gatgtgaaac
    32161 tgaacaacct cagtgggccg atctctatcg ccaagggggc tgggatgaca gcggaactcg
    32221 gggtagttta ttacctgccg tttcttgcgc ttattagcgt gaacttaggg ataattaacc
    32281 tgtttccgtt gcccgtactt gacggggggc atctgctgtt ccttgcgatc gaaaagatca
    32341 agggcggacc ggtatccgag cgggttcaag acttttgtta tcgcattggc tcgattctgc
    32401 tggtgctgtt aatggggctt gcacttttca atgatttctc tcggttatga gagttagtta
    32461 ggaagaacgc ataataacga tggcgatgaa aaagttgctc atagcgtcgc tgctgtttag
    32521 cagcgccacc gtatacggtg ctgaagggtt cgtagtgaaa gatattcatt tcgaaggcct
    32581 tcagcgtgtc gccgttggtg cggccctcct cagtatgccg gtgcgtacag gcgacacggt
    32641 taatgatgaa gatatcagta ataccattcg cgctctgttt gctaccggca actttgagga
    32701 tgttcgcgtc cttcgtgatg gtgataccct tctggttcag gtaaaagaac gtccgaccat
    32761 tgccagcatt actttctccg gtaacaaatc ggtgaaagat gacatgctga agcaaaacct
    32821 cgaggcttct ggtgtgcgtg tgggcgaatc cctcgatcgc accaccattg ccgatatcga
    32881 gaaaggtctg gaagacttct actacagcgt cggtaaatat agcgccagcg ttaaagctgt
    32941 cgtgaccccg ctgccgcgca accgtgttga cctaaaactg gtgttccagg aaggtgtgtc
    33001 agctgaaatc cagcaaatta acattgttgg taaccatgct ttcaccaccg acgaactgat
    33061 ctctcatttc caactgcgtg acgaagtgcc gtggtggaat gtggtaggcg atcgtaaata
    33121 ccagaaacag aaactggcgg gcgatcttga aaccctgcgc agctactatc tggaccgcgg
    33181 ttatgcccgt ttcaacatcg actctaccca ggtcagtctg acgccagata aaaaaggtat
    33241 ttacgtcacg gtgaacatca ccgaaggcga tcagtacaag ctttctggcg ttgaagtgag
    33301 cggcaacctt gccgggcact ccgctgaaat tgagcagctg actaagatcg agccgggcga
    33361 gctgtataac ggcactaaag tgaccaaaat ggaagatgac attaaaaagc ttctcggtcg
    33421 ctatggttat gcctatccgc gcgtacagtc gatgcctgaa attaacgatg ccgacaaaac
    33481 cgttaaatta cgcgtgaacg ttgatgtggg taaccgtttc tacgtgcgta agatccgttt
    33541 tgaaggtaac gatacctcga aagatgccgt cctgcgtcgc gaaatgcgtc agatggaagg
    33601 tgcatggttg gggagcgatc tggtcgatca gggtaaggag cgtctgaatc gtctgggctt
    33661 ctttgaaact gtcgataccg atacccaacg tgttccgggt agcccggacc aggttgatgt
    33721 agtctacaag gtgaaagaac gtaacactgg tagcttcaac ttcgggattg gttacggtac
    33781 tgaaagtggc gtgagcttcc aggctggcgt gcaacaggat aactggttag gtacaggtta
    33841 tgctgttggt atcaacggga ccaaaaacga ttaccagacc tatgctgaac tgtcggtaac
    33901 caacccgtac ttcaccgtag atggcgtaag cctcggtggt cgtctcttct ataatgactt
    33961 ccaggcagat gacgccgacc tgtccgacta taccaacaag agttatggta cagacgtgac
    34021 gttgggcttc ccgattaacg aatataactc gctgcgtgca ggtctgggtt atgtacataa
    34081 ctccctgtcc aacatgcagc ctcaggttgc gatgtggcgt tatctgtact ctatgggtga
    34141 acatccgagc acctctgatc aggataacag cttcaaaacg gacgacttca cgttcaacta
    34201 tggttggacc tataacaagc ttgaccgtgg ttacttcccg acagatggtt cacgtgtcaa
    34261 cctgaccggt aaagtgacca ttcctggatc ggataacgaa tactacaaag tgacgttaga
    34321 cacggcgact tatgtgccga tcgatgacga tcacaaatgg gttgttctgg gtcgtacccg
    34381 ctggggttat ggtgatggtt taggcggcaa agagatgccg ttctacgaga acttctatgc
    34441 cggtggttcc agcaccgtgc gtggcttcca gtccaatacc attggtccga aagcagttta
    34501 cttcccgcat caggccagta attatgatcc ggactatgat tacgaatgtg cgactcagga
    34561 cggcgcgaaa gacctgtgta aatcggatga tgctgtaggc ggtaacgcca tggcggttgc
    34621 cagcctcgag ttcatcaccc cgacgccgtt tattagcgat aagtatgcta actcggttcg
    34681 tacttcattc ttctgggata tgggtaccgt ttgggataca aactgggatt ccagccaata
    34741 ttctggatat ccggactata gtgatccaag caatatccgt atgtctgcgg gtatcgcatt
    34801 acaatggatg tccccattgg ggccgttggt gttctcctac gcccagccgt tcaaaaagta
    34861 cgatggagac aaggcagaac agttccagtt taacatcggt aaaacctggt aagtgttctc
    34921 cacaaaggaa tgtagtggta gtgtagcgat gactttaggc gatcaatata agatcgccgg
    34981 gccacgcaaa gaactgcacc ctccggtgca aatgggatgg taaggagttt attgtgaaaa
    35041 agtggttatt agctgcaggt cttggtttag cactggcaac ttctgctcag gcggctgaca
    35101 aaattgcaat cgtcaatatg ggcagcctgt tccagcaggt agcgcagaaa accggtgttt
    35161 ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga actgcagcgt atggaaaccg
    35221 atctgcaggc taaaatgaaa aaactgcagt ccatgaaagc gggcagcgat cgcaccaaac
    35281 tggaaaaaga cgtgatggct cagcgccaga cttttgctca gaaagcgcag gcttttgagc
    35341 aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact ggttactcgt atccagactg
    35401 ctgtgaaatc cgttgccaac agccaggata tcgatctggt tgttgatgca aacgccgttg
    35461 cttacaacag cagcgatgta aaagacatca ctgccgacgt actgaaacag gttaaataag
    35521 taatgccttc aattcgactg gctgatttag cgcagcagtt ggatgcagaa ctacacggtg
    35581 atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc tgcacaaaca ggtcacatta
    35641 cgttcatggt taacccaaaa taccgtgagc atttaggctt gtgccaggcg tccgcggttg
    35701 tcatgaccca ggacgatctt cctttcgcga aaagtgccgc gctggtagtg aagaatccct
    35761 acctgactta cgcgcgcatg gcgcaaattt tagataccac gccgcagccc gcgcagaaca
    35821 ttgcacccag tgcggtgatc gacgcgacgg cgaagctggg taacaacgta tcgattggcg
    35881 ctaacgcggt gattgagtcc ggcgttgaac tgggcgataa cgtgattatc ggtgccggtt
    35941 gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg tctctgggcg aacgtaacca
    36001 tttaccatga gatccagatc ggtcagaatt gcctgatcca gtccggaaca gtggtaggcg
    36061 cagacggctt tggttatgcc aacgatcgtg gtaactgggt gaagatccca cagattggtc
    36121 gcgtaattat tggcgatcgc gtggagatcg gtgcctgcac aaccatcgat cgcggcgcgc
    36181 tggatgacac tgttattggc aatggcgtga tcattgataa ccagtgccag attgcacata
    36241 acgtcgtgat tggcgacaat acggcggttg ccggtggcgt cattatggcg ggcagcctga
    36301 aaattggtcg ttactgcatg atcggcggag ccagcgtaat caacgggcat atggaaatat
    36361 gcgacaaagt gacggttacg ggcatgggta tggtgatgcg tcccatcact gaaccaggcg
    36421 tctattcctc aggcattccg ctgcaatcca acaaagtctg gcgcaaaacc gctgcactgg
    36481 tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct tgagcgcaag gttaatcaac
    36541 aagactaacg ttccatcttt tgttcgccaa actttacggc ctgtctcatt cttacgattg
    36601 cggcaggccg tgttattatt gtcgtttctt atattttgac aggaagagta tcttgactac
    36661 taacactcat actctgcaga ttgaagagat tttagaactt ctgccgcacc gtttcccgtt
    36721 cttactggtg gatcgcgtgc tggattttga agaaggtcgt tttctgcgcg cagtaaaaaa
    36781 tgtctctgtc aatgagccat tcttccaggg ccatttccct ggaaaaccga ttttcccggg
    36841 tgtgctgatt ctggaagcaa tggcacaggc aacaggtatt ctggcgttta aaagcgtagg
    36901 aaaactggaa ccgggtgagc tgtactactt cgctggtatt gacgaagcgc gcttcaagcg
    36961 cccggtcgtg cctggcgatc aaatgatcat ggaagtcact ttcgaaaaaa cgcgccgcgg
    37021 cctgacccgt tttaaagggg ttgctctggt cgatggtaaa gtagtttgcg aagcaacgat
    37081 gatgtgtgct cgtagccggg aggcctgata cgtgattgat aaatccgcct ttgtgcatcc
    37141 aaccgccatt gtggaagagg gcgcgtcaat tggcgcgaac gcacacattg gtcctttttg
    37201 tatcgttgga ccccatgtcg aaattggtga gggtaccgta ctgaaatctc acgttgtcgt
    37261 gaatggtcat actaaaattg gccgcgataa tgagatttat cagtttgcct ccatcggcga
    37321 agttaaccag gatttgaaat atgctggcga accgacccgt gtggaaatcg gcgatcgtaa
    37381 ccgcattcgc gaaagcgtca ccattcatcg tggcacagtc cagggcggtg gattgacgaa
    37441 ggtgggcagc gacaacttac tgatgatcaa cgcgcacatt gcgcacgatt gtacggtagg
    37501 taaccgctgt attctcgcca acaacgcaac gctggcgggt cacgtatcgg ttgacgactt
    37561 cgcgatcatc ggcggcatga ccgcagtcca tcagttctgc atcattggtg cgcatgtgat
    37621 ggttggcggc tgctccggtg tggcgcagga cgtccctcct tatgtcattg cgcagggtaa
    37681 ccacgcaacg ccgttcggtg tcaatatcga agggctgaag cgccgcggat tcagccgtga
    37741 ggcgattacc gctatccgca atgcgtataa gctaatttat cgtagcggta aaacgctcga
    37801 tgaagtgaaa ccggaaattg ctgaactggc ggaaacatat ccggaagtga aagcctttac
    37861 cgatttcttt gcacgctcaa cgcgcggtct gattcgttaa tgactgacca gcgtccatta
    37921 acgattgccc tggtcgccgg agaaacctcc ggcgatatcc tgggggccgg tttaatccgc
    37981 gctctgaaag aacgtgtgcc caacgcccgc tttgttggtg ttgccgggcc acgaatgcag
    38041 gctgaaggct gcgaagcctg gtacgaaatg gaagaactgg cggtgatggg cattgttgaa
    38101 gtgctcggtc gtctgcgtcg cttactgcat attcgtgccg atctgacaaa gcgttttggc
    38161 gaactgaagc cagatgtttt tgttggtatt gatgcgcccg acttcaatat tactcttgaa
    38221 ggtaacctca aaaagcaggg tatcaaaacc attcattacg tcagtccatc cgtctgggcg
    38281 tggcgacaga aacgcgtttt caaaataggc agagccaccg atctggtgct cgcatttctg
    38341 cctttcgaaa aagcgtttta tgacaaatac aatgtaccgt gccgctttat cggtcatacc
    38401 atggctgatg ccatgccgtt agatccagat aaaaatggtg cccgtgatgt gctggggatc
    38461 ccttacgatg cccactgtct ggcattgttg ccgggcagcc gtggtgcaga agtcgaaatg
    38521 cttagtgccg atttcttgaa aactgcccag cttttgcgcc agacatatcc ggatctcgaa
    38581 atcgtggtgc cgctggtgaa tgccaaacgc cgcgagcagt ttgaacgcat caaagctgaa
    38641 gtcgcgccag acctgtcggt tcatttgctg gatggaatgg gccgtgaggc gatggtcgcc
    38701 agcgatgcgg cactactggc gtcggggacg gcagccctgg agtgtatgct ggcgaaatgc
    38761 ccgatggtgg tgggatatcg catgaagcct tttaccttct ggttggcgaa gcggctggtg
    38821 aaaactgatt atgtctcgct gccaaatctg ctggcgggca gagagttagt caaagagtta
    38881 ttgcaggaag agtgtgagcc gcaaaaactg gctgcggcgc tgttaccgct gttggcgaac
    38941 gggaaaacca gccacgcgat gcacgatacc ttccgtgaac tgcatcagca gatccgctgc
    39001 aatgccgatg agcaggcggc acaagccgtt ctggagttag cacaatgatc gaatttgttt
    39061 atccgcacac gcagctggtt gcgggtgtgg atgaagtcgg acgcgggccg ttagttggcg
    39121 cggtcgtcac cgctgcggtg atccttgacc cggcgcgccc gattgccggg ctgaatgatt
    39181 ccaaaaagct gagcgaaaaa cgccgtctgg cgctctatga agagatcaaa gagaaagcgt
    39241 tgagctggag tctgggccgc gcggaacccc acgaaatcga cgagctgaac attcttcatg
    39301 cgaccatgct ggcgatgcag cgtgccgtcg ctgggctgca tattgcgccg gaatatgtgt
    39361 tgattgatgg taaccgctgc ccgaaattac cgatgcctgc gatggctgtg gtgaaagggg
    39421 atagccgcgt accagaaatc agtgccgcgt ctatcctggc gaaagtgacg cgtgacgccg
    39481 aaatggcggc gctggatatt gttttcccgc aatatggttt tgcccaacat aaagggtatc
    39541 caaccgcttt tcatctggaa aaactggctg aatatggcgc gactgaacat catcgtcgca
    39601 gctttgggcc tgtcaaacgc gcactgggac ttgcgtcctg attcttgtgt cgagattaag
    39661 taaaccggaa tctgaagatg tctgaaccac gtttcgtaca cctgcgggtg cacagcgact
    39721 actcgatgat cgatggcttg gccaaaaccg caccgctggt aaaaaaggcg gcggcgttgg
    39781 gtatgccagc actggcgatc accgatttca ccaacctttg cggtctggtg aagttctacg
    39841 gagcgggaca tggcgcaggg attaagccta tcgtcggggc agattttaat gtccagtgcg
    39901 acctgctggg tgatgagtta acccacctga cggtactggc ggcgaacaat accggctatc
    39961 agaatctggc gttgctgatc tcaaaagcgt atcagcgcgg gtacggtgcc gccgggccga
    40021 tcatcgatcg cgactggctt atcgaattaa acgaagggtt gatccttctt tccggcgggc
    40081 gcatgggcga cgtcggacgc agtcttttgc gtggtaacag cgcgctggta gatgagtgtg
    40141 tcgcgtttta tgaagaacac ttcccggatc gctattttct cgagctgatc cgcaccggca
    40201 ggccggatga agaaagctat ctgcacgcgg cggtggaact ggcggaagcg cgcggtttgc
    40261 ccgtcgtggc gactaacgat gtgcgcttta tcgacagcag cgactttgac gcacacgaaa
    40321 tccgcgtcgc gatccacgac ggctttaccc tcgacgatcc taaacgcccg cgtaactatt
    40381 cgccgcagca atatatgcgt agcgaagagg agatgtgtga gctgtttgcc gacatcccag
    40441 aagcccttgc caacaccgtt gagatcgcca aacgctgtaa cgtaactatg cgtcttggtg
    40501 aatacttcct gccgcagttc ccgaccgggg acatgagcac cgaagattat ctggtcaagc
    40561 gtgcaaaaga aggcctggaa gagcgtctgg catttttatt ccctgacgag gaagaacgtc
    40621 ttaaacgccg cccggaatat gacgaacgtc tggagactga acttcaggtt atcaaccaga
    40681 tgggcttccc gggctacttc ctcatcgtta tggaatttat ccagtggtcg aaagataacg
    40741 gcgtaccggt agggccaggc cgtggctccg gtgcgggttc actggtggcc tacgcgctga
    40801 aaatcaccga cctcgatccg ctggaatttg acctgctgtt cgaacgtttc cttaacccgg
    40861 aacgtgtctc catgcctgac ttcgacgttg acttctgtat ggagaaacgc gatcaggtta
    40921 tcgaacacgt ggcggacatg tacggtcgtg atgcggtatc gcagattatc accttcggta
    40981 cgatggcggc gaaagcggtg atccgcgacg taggccgcgt gctggggcat ccgtacggct
    41041 ttgtcgaccg tatctcgaaa ctgatcccgc ccgatccggg gatgacgctg gcgaaagcgt
    41101 ttgaagccga gccgcagctg ccggaaatct acgaagcgga tgaagaagtt aaggcgctga
    41161 tcgacatggc gcgcaaactg gaaggggtca cccgtaacgc cggtaagcac gccggtgggg
    41221 tggttatcgc gccgaccaaa attaccgatt ttgcgccgct ttattgcgat gaagagggca
    41281 aacatccggt cacccagttt gataaaagcg acgttgaata cgccggactg gtgaagttcg
    41341 acttccttgg tttgcgtacg ctcaccatca tcaactgggc gctggagatg atcaacaagc
    41401 ggcgggcgaa gaatggcgag ccatcgctgg atatcgccgc gatcccgctg gatgacaaga
    41461 aaagcttcga catgctgcaa cgctcggaaa ccacggcggt attccagctt gaatcgcgcg
    41521 gcatgaagga cctgatcaag cgtctgcaac ctgactgctt cgaagatatg atcgcactgg
    41581 tggcactgtt ccgccctggt ccgttgcagt cagggatggt ggataacttt atcgaccgta
    41641 aacatggtcg tgaagagatc tcctatccgg acgtgcagtg gcagcatgaa agcctgaaat
    41701 cggtactgga gccaacctac ggcattatct tgtatcagga acaggtcatg cagattgccc
    41761 aggtgctttc tggttatacc ctcggtggcg cggatatgct gcgccgtgcg atgggtaaga
    41821 aaaagccgga agagatggct aagcagcgtt ctgtatttgc tgaaggtgca gaaaagaacg
    41881 gaatcaacgc cgaactggcg atgaaaatct tcgacctggt ggagaaattc gcgggttacg
    41941 gatttaacaa atcgcactct gcggcctatg ctttggtgtc atatcaaacg ttatggctga
    42001 aagcgcacta tccggcggag tttatggcgg cggtaatgac cgccgatatg gacaacaccg
    42061 agaaggtggt gggcctggtg gatgagtgct ggcggatggg gctgaaaatc ctgccaccag
    42121 atataaactc cggtctttac catttccacg tcaacgacga cggcgaaatc gtgtatggta
    42181 ttggcgccat caaaggggta ggtgaaggtc cgattgaggc catcatcgaa gcccgtaata
    42241 aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg taccgacacc aaaaagttaa
    42301 accggcgggt gctggaaaaa ctgatcatgt ccggggcgtt tgaccgtctt gggccacacc
    42361 gcgcggcgct gatgaactcg ctgggtgatg cgttaaaagc ggcagatcaa cacgcgaaag
    42421 cggaagctat cggtcaggcc gatatgttcg gcgtgctggc cgaagagccg gaacaaattg
    42481 aacaatccta cgccagctgc caaccgtggc cggagcaggt ggtattagat ggggaacgtg
    42541 aaacgttagg cctgtacctg accggacacc ctatcaacca gtatttaaaa gagattgagc
    42601 gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac agaacgtggt aaagtcatca
    42661 cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac caagcgcggc aatcgtatcg
    42721 gtatctgcac gctggatgac cgttccgggc ggctggaagt gatgttgttt actgacgccc
    42781 tggataaata ccagcaattg ctggaaaaag accgcatact tatcgtcagc ggacaggtca
    42841 gctttgatga cttcagcggt gggcttaaaa tgaccgctcg cgaagtgatg gatattgacg
    42901 aagcccggga aaaatatgct cgcgggcttg ctatctcgct gacggacagg caaattgatg
    42961 accagctttt aaaccgactc cgtcagtctc tggaacccca ccgctctggg acaattccag
    43021 tacatctcta ctatcagagg gcggatgcac gcgcgcggtt gcgttttggc gcgacgtggc
    43081 gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg cctcattggt tcggagcagg
    43141 tggaactgga gtttgactaa tacaggaata ctatgagtct gaatttcctt gattttgaac
    43201 agccgattgc agagctggaa gcgaaaatcg attctctgac tgcggttagc cgtcaggatg
    43261 agaaactgga tattaacatc gatgaagaag tgcatcgtct gcgtgaaaaa agcgtagaac
    43321 tgacgcgtaa aatcttcgcc gatctcggtg catggcagat tgcgcaactg gcacgccatc
    43381 cgcagcgtcc ttataccctg gattacgttc gcctggcatt tgatgaattt gacgaactgg
    43441 ctggcgaccg tgcgtatgca gacgataaag ctatcgtcgg tggtatcgcc cgtctcgatg
    43501 gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga aaccaaagaa aaaattcgcc
    43561 gtaactttgg tatgccagcg ccagaaggtt accgcaaagc actgcgtctg atgcaaatgg
    43621 ctgaacgctt taagatgcct atcatcacct ttatcgacac cccgggggct tatcctggcg
    43681 tgggcgcaga agagcgtggt cagtctgaag ccattgcacg caacctgcgt gaaatgtctc
    43741 gccttggcgt accggtagtt tgtacggtta tcggtgaagg tggttctggc ggtgcgctgg
    43801 cgattggcgt gggcgataaa gtgaatatgc tgcaatacag cacctattcc gttatctcgc
    43861 cggaaggttg tgcgtccatt ctgtggaaga gcgccgacaa agcgccgctg gcggctgaag
    43921 cgatgggtat catcgctccg cgtctgaaag agctgaaact gatcgactcc atcatcccgg
    43981 aaccgctggg cggcgctcac cgtaacccgg aagcgatggc ggcatcgttg aaagcgcaac
    44041 tgctggcgga tctggccgat ctcgatgtgt taagcactga agatttaaaa aatcgtcgtt
    44101 atcagcgcct gatgagctac ggttacgcgt aattcgcaaa agttctgaaa aagggtcact
    44161 tcggtggccc ttttttatcg ccacggtttg agcaggctat gattaaggaa ggattttcca
    44221 ggaggaacac atgaacatca ttgccattat gggaccgcat ggcgtcttct ataaagatga
    44281 gcccatcaaa gaactggagt cggcgctggt ggcgcaaggc tttcagatta tctggccaca
    44341 aaacagcgtt gatttgctga aatttatcga acataaccct cgaatttgcg gcgtgatttt
    44401 tgactgggat gagtacagtc tcgatttatg cagcgatatc aatcaactta atgaatatct
    44461 cccgctttat gccttcatca acacccactc gacgatggat gtcagcgtgc aggatatgcg
    44521 gatggcgctc tggttttttg aatatgcgct ggggcaggcg gaagatatcg ccattcgtat
    44581 gcgtcagtac accaacgaat atcttgataa cattacgccg ccgttcacga aagccttgtt
    44641 tacctacgtc aaagagcgga agtacacctt ttgtacgccg gggcatatgg gcggcaccgc
    44701 atatcaaaaa agcccggttg gctgtctgtt ttatgatttt ttcggcggga atacccttaa
    44761 ggctgatgtc tctatttcgg tcaccgagct tggttcgttg cttgaccaca ccgggccaca
    44821 cctggaagcg gaagagtaca tcgcgcggac ttttggcgcg gaacagagtt atatcgttac
    44881 caacggaaca tcgacgtcga acaaaattgt gggtatgtac gccgcgccat ccggcagtac
    44941 gctgttgatc gaccgcaact gccataaatc actggcgcat ctgttgatga tgaacgatgt
    45001 agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg attctggggg ggatcccgcg
    45061 ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct gccactacgc aagcacaatg
    45121 gccggttcat gcggtgatca ccaactccac ctatgatggt ttgctctaca acaccgactg
    45181 gatcaaacag acgctggatg tcccgtcgat ccacttcgat tccgcctggg tgccgtatac
    45241 tcattttcat ccaatctacc agggtaaaag tggtatgagc ggcgagcgtg ttgcgggaaa
    45301 agttttcttc gaaacgcaat cgacccacaa aatgctggcg gcgttatcgc aggcttcact
    45361 gatccacatt aaaggcgagt atgacgaaga ggcgtttaac gaagccttta tgatgcatac
    45421 caccacctcg cccagttatc ccattgttgc ttcggttgag acggcggcgg cgatgctgcg
    45481 tggtaatccg ggcaaacggc tgattaaccg ttcggtggag cgggcgctgc attttcgcaa
    45541 agaggtccag cggctgcggg aagagtctga cggttggttt ttcgatatct ggcaaccgcc
    45601 gcaggtggat gaagccgaat gcaggcccgt tgcgcctggc gaacaatggc acggctttaa
    45661 cgatgcggat gccgatcata tgtttctcga tccggttaaa gtcactattt tgacaccggg
    45721 gatggacgaa cagggcaata tgagtgagga ggggatcccg gcggcgctgg tagcaaaatt
    45781 cctcgacgaa cgtgggatcg tagtagagaa aaccggccct tataacctgc tgtttctctt
    45841 tagtattggc atcgataaaa ccaaagcaat gggattattg cgtgggttga cggaatttaa
    45901 gcgctcttac gatctcaacc tgcggatcaa aaatatgctg cccgatctct atgctgaaga
    45961 tcccgatttc taccgcaata tgcgtattca ggatctggcg caagggatcc ataagctgat
    46021 tcgtaaacac gatctttccg gtttgatgtt gcgggcattc gatactttgc cggaaatgat
    46081 catgacgcca catcaggcat ggcagagaca gattaaaggt gaagtagaaa ccattgcgct
    46141 ggaacaactg gtcggtagag tatcggcaaa tatgatcctg ccttatccac cgggcgtacc
    46201 gctgttgatg ccaggagaaa tgctgaccaa agagagccgc acggtactcg attttctact
    46261 gatgctttgt tccgtagggc aacattaccc cggttttgaa acggatattc acggcgcgaa
    46321 acaggacgaa gacggcgttt accgcgtacg agtcctaaaa atggcgggat aacttgccag
    46381 agcggcttcc gggcgagtaa cgtgctgtta acaaataaag gagacgttat gctgggttta
    46441 aaacaggttc accatattgc gattattgcg acggattatg cggtgagcaa agctttctac
    46501 tgcgatattc ttggtttcac gctgcaaagc gaagtctatc gcgaagcgcg cgactcgtgg
    46561 aaaggggatt tggcgcttaa tgggcaatat gtgattgagc ttttctcttt tccgttcccg
    46621 ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc atctggcttt tagcgttgat
    46681 gacatcgatg cggcagtggc gcaccttgaa agccataacg tgaagtgtga agccatccgt
    46741 gtcgatccat acacgcaaaa acgcttcacc ttctttaacg atccggacgg gctgccgttg
    46801 gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc ccggtaacgt gccgggcatt
    46861 gctactgtaa aatcgcacca tcatgacact cacgctcaat agacaacttc tcacctcacg
    46921 ccagattctg gtggccttta gcggcgggct tgactccacc gttctgctgc atcagttggt
    46981 gcagtggcgg acggaaaatc cgggcgtcac tctgcgcgct attcatgtgc atcacggttt
    47041 aagtgccaat gccgatgcct gggttacgca ttgcgaaaac gtctgccaac agtggcaggt
    47101 gccgctggtg gtcgaacgcg tacaacttgc gcaagagggg ctggggattg aggcccaggc
    47161 gcggcaggca cgttatcagg catttgcccg taccctattg cccggcgaag tgctggtcac
    47221 cgcgcaacat ctcgacgatc aatgtgaaac ctttctgctg gcgctaaaac gcggtagcgg
    47281 ccctgccggg ctttcggcta tggcggaagt ttcggagttt gctggaacac ggcttatccg
    47341 cccgttgctt gcccgcacgc ggggggaact ggagcagtgg gcattggcgc atggtttacg
    47401 ctggattgaa gacgaaagta atcaggacga ctcatacgat cgtaactttc tgcgcctgcg
    47461 cgtagtgccg ttattgcagc agcgttggcc gcattttgcg gaagcaacgg cccgcagcgc
    47521 cacactttgt gctgaacaag agagtctgtt ggatgaactg ctggcggatg atttagcaca
    47581 ctgccaaacg tcgcagggga cgctgcagat tgcgccaatg ctggcgatga gtgatgcccg
    47641 tcgcgcggcg attattcgcc gctggctggc agggcagaat gcaccgatgc cttcccgcga
    47701 cgcgctggtg cgcatctggc aggaagtggc gctggcgcgg gaagatgctt ccccctgttt
    47761 acgtttgggc gcctttgaaa tccgccgcta tcagtcgcaa ctgtggtgga ttaaatccgt
    47821 caccgggcaa agcgaaacca ttgtgctgtg gcagacgtgg ctacaaccgc tggaattacc
    47881 ggcggggctg ggaaccgtac agcttactgc gggaggcgat attcgccctc cgcgtgcaga
    47941 cgaagcggtc agcgtgcgtt tcaaagcgcc aggattgctg catattgtcg ggcgcaacgg
    48001 cggacgtaag ctgaagaaaa tctggcaaga gttaggcgtg ccgccgtggc tacgtgacac
    48061 cacgccactg ctgttttatg gagaaacgct gattgcggcg gcaggggtat ttgtgacgca
    48121 agaaggtgtg gctgaaggtg agaatggcgt aagttttgtc tggcagaaaa cgcttagtta
    48181 agtgaaagcc ggataagacg catcaaacgt cgcatccggc gaaagtcaat caggattcgc
    48241 ttaccaccac cgtaccgatt tcagggtggc taaagctggt aattttatcc agacgcagct
    48301 cgcgggtttc gccagcggcc tcgacgacca gatactccac atttttgcgg gagactaaat
    48361 cactggcttt tgcctgcaat ttttcgccat ctttcagctc aagtgtcagc attaaatgat
    48421 gctggcaggc gagctcaaga ttatcgtaat catcacaatt aattggttga tacgtatcat
    48481 tcattgacat aatcgctcac cagtaagttt gccgcagcgt atgctgcttt ttccctgaca
    48541 gcctcagaaa gggcgtcgtc ggcagccatt tcattcagca ctttcaaaac gcagcccagc
    48601 gcgtccggaa cgtatcctaa gtctccgctg gcgatttccg cgtaccgctt gcgtattaac
    48661 tcacaatatt tttccacatg ccctcctgtc agcactctga cttaaccgtg gatgcaagtc
    48721 taagcctacg aagataaact ctgtttcgca aggtgactat accacactca tttctgcaat
    48781 atcagcgccg caactgcacg tattccgtta caatggcctc ctgattcgaa aggagttttc
    48841 ttatggcgct taaagcgaca atttataaag cgacggttaa tgtggccgat ctcgaccgca
    48901 accagtttct cgatgcctct ctgacgctgg cgcgccaccc ttcagaaacc caggagcgta
    48961 tgatgctgcg cttgctggcg tggctgaaat atgctgatga acgtctgcaa tttacccgtg
    49021 gtttgtgtgc cgatgatgag ccggaagcgt ggctgcgtaa cgatcacctg ggcattgatt
    49081 tgtggattga gctggggctg ccggatgagc gacgaattaa gaaagcctgc acccaggccg
    49141 cagaagtggc gctgtttacc tataatagtc gggcggcgca aatctggtgg cagcaaaatc
    49201 agagcaaatg tgtgcagttt gccaatcttt ccgtctggta tctggacgat gaacaactgg
    49261 cgaaagtaag cgcctttgcc gatcgtacca tgacgctgca ggcaacgatt caggatggtg
    49321 ttatctggtt atcggatgat aagaataatc tggaagttaa cttaaccgcc tggcaacaac
    49381 cttcatgatt gtgatttccc gacatgttgc tattcccgat ggtgagcttg agatcaccgc
    49441 cattcgtgcg cagggcgctg gcgggcagca tgttaataag acctcaacgg ctattcatct
    49501 gcgttttgac attcgggcgt ccagcctgcc agagtattac aaagagcgtc tgctcgccgc
    49561 cagccatcat ttgatcagca gtgatggcgt gattgtcatt aaggcacagg aataccgcag
    49621 tcaggaactg aaccgcgaag ccgctctggc ccggctggtg gcagtgatta aagatttaac
    49681 aaaagaacaa aaagcccgac gacccacgcg gcccacccgt gcatcgaaag agcgcaggct
    49741 ggcatcgaaa gcacaaaaat caagcgtgaa ggcgatgcgc ggcaaagtac gcagcggtcg
    49801 ggaataaaaa gaaggaatgg atggtgaaaa aagcgatagt gacagcgatg gctgtaatca
    49861 gcctctttac tctgatggga tgtaataatc gggccgaagt cgatacgctt tctccggcgc
    49921 aggctgccga actgaaaccg atgccgcaaa gttggcgcgg cgtgctaccg tgtgccgatt
    49981 gcgaaggaat cgaaatctct ctgttcctcg aaaaagacgg aacatgggtg atgaatgagc
    50041 gttatctcgg tgctcgtgaa gaaccttcct ccttcgcttc ctacggtaca tgggcgcgaa
    50101 ccgctgacaa gctggtatta accgacagca aaggtgaaaa gtcatattat cgggcgaaag
    50161 gcgatgcgct ggagatgctc gatcgtgaag gcaatccaat tgaatcgcag ttcaactata
    50221 cgctggaacc ggcacaatcc agtttaccta tgacgccgat gaccctgcga ggcatgtatt
    50281 tttatatggc tgatgcggcg accttcactg attgcgcgac cggaaaacgt ttcatggtag
    50341 cgaataacgc agagctggag cgtagctacc tggctgcgcg cggtcacagt gaaaaaccgg
    50401 tgttactgtc agtagaaggt cactttacgc ttgaggctaa tccggatact ggtgcgccaa
    50461 ccaaagtatt agcgcccgat acggcgggta aattttaccc gaataaggat tgcagtagtt
    50521 tggggcagta aacctgctta aggcagaaac aaacgcaaaa ttgcctgatg cgctgcgctt
    50581 atcaggccta cagcatataa tgcaacattt tgaattagca cgattttgta ggccggataa
    50641 ggcgtttacg ccgcatccgg cattttgtgc acgaggctgg ctttatcact gagtcagccc
    50701 aacaaagcga cccgccttga tataaatccc aggcttcagg tgcccaacat actgtaactg
    50761 cgtttctggc ttaaacgcag agacatcacc aatacgcata tgcatcagat cggcagggct
    50821 aatccagccc gattgcgcca gtgtaagtgg atgcccggat ttggcaaatg catcagtgac
    50881 aaattccgaa caaaaccacg actttttgtc tccttcgcac acactgctta actgcgcttt
    50941 cgccaggtcg ctgacgcact gttggcgaaa atcctcggag aacggattca gtgagcacat
    51001 ctggcgagtc accataaagg gaataaattc gacaatgccg cgatagttat aaccgctatc
    51061 tttgatttta ttggcaaacg cggtgatatc tgtggcttgt tgcggggtaa gatccgggac
    51121 tcgtaagacg aaaagcttat cactatgctt catcgctttt ttaagggaaa caatctggac
    51181 gccagcgcct gtcgcttctg caacgttatt atcacccaga aagattgcaa cgtgactcac
    51241 agaggaagtg ctgaagacgc ggattccaaa tgaggttacc ccaaggcttg aggagaacag
    51301 caaatcgccg ggtttgagat caggtgccgt tatttctttt attgattgtt cggtgaaaga
    51361 gctttgatgc tggaatttga cagcccatgt ttttgcctcg gcatctactg ctgtggctga
    51421 tgggtctggc tggctgatat caacggtaca agccgaaagt aaaagaaagc tggggagaag
    51481 caggcggcag tacgcctttg gtttatccat tttatacaat ccatgtaaaa aagggccctg
    51541 aaattcagga ccctttctgg catcagcctt taatctgttt caccagatat tcgacgatgt
    51601 caccagtctt aattaactgt ttctcgccgt tgcgacgata tttatattcg atatcgtcgt
    51661 tgtcgaggtt acggtcgccc agcacaatag tgtgcggaat accgatcagt tccatatcag
    51721 caaacatcac gcccggacgc tctttgcggt catccagcag cacttcgata ccttgtgcac
    51781 gcagttcgct gtacagtttc tcagcaagct cttgtacgcg gaaggatttg tgcatgttca
    51841 tcggcagaat cgccacctgg aacggcgcga tagcgtcagg ccatacgatg ccgcgttcgt
    51901 cgtagttctg ctcaatcgcc gcagctacca cacgcgttac cccgataccg tagcaaccca
    51961 tcgtcaggat ttggttacgg ccatcttcac cctgtacgga ggctttcagt gcttcggagt
    52021 acttggtacc cagctggaag atgtgaccaa cttcgatacc acgtttgatc agcagcgtac
    52081 cctggccatc cgggcttgga tcgccagcca ccacgttacg gatatctgca acttccgggg
    52141 tagcgacatc gcgatcccag ttgataccga agtagtgttt accatcgatg ttagcaccag
    52201 cagcgaaatc actcatcgcc gcaacggtac ggtcaatcac caccggaatc ggcatgttta
    52261 ccggacccag tgaacccgga ccggctttaa ccacagcacg aatttcttct tcggtcgcga
    52321 aagtcagcgg gcttgcaacc tgcggcagtt tttctgcttt aacttcgttc agctcgtgat
    52381 caccgcgcac cagcagcgca accagcggga agctactgcc ttcaaccgct ttaaccagca
    52441 gagtcttaac cgttttctca atcggcagat tgaactgttc aaccagttcc gcgatggttt
    52501 tcgcgttcgg cgtatcaacc agcgtcattt cctgggtagc agcagcgcgc agttctttcg
    52561 gcgcgatagc ttctgccagt tcaatgttcg ctgcatagtc agaggtgtcg gagaagacca
    52621 catcgtcttc accgctctgc gccagcacct ggaattcgtg agaggcgctg ccgccgatag
    52681 aaccggtgtc ggcctgtacg gcgcggaaat ccagccccat gcggctgaag attttgctgt
    52741 aggccgcata cattgcatcg taggtttcct gcagggattc ctgagaagta tggaaagagt
    52801 aagcatcttt catcaggaat tcgcgggaac gcatgacgcc gaaacgcgga cgcacttcgt
    52861 cgcggaactt ggtctggatc tgatagaagt tcagcggcag ctgtttgtaa gagctaagct
    52921 cgttacgaat caggtcagtg ataacttctt catgagttgg gccgagtacg aacggacgct
    52981 cgccacggtc aacaaaacgc agcagttccg gaccgtactg ttcccaacga ccactctctt
    53041 gccacaaatc ggctggctga accaccggca tcgacacctc gatcgcaccg gcgttgttca
    53101 tctcttcacg cacgatgttt tcgacttttt tcagaacgcg cacgccggtc ggcagccagg
    53161 tatataaccc ggaggccagc ttgcggatca tcccggcgcg cagcatcagc tgatggctga
    53221 tcacctcggc gtcggcaggt gtctccttga gagtggagag caggtattgg ctagtacgca
    53281 tgttgttacg gttccagttg gaaggtagaa caggctcaag gcgagcctgg gacaaaaaaa
    53341 gtgatttagt ttaccagtgc aaaagaaatg tcaaaagaga agggcgtgaa tttaacgcgg
    53401 ttccagcgca aagacttcaa aacctgcgtc ggtgacgcgc cagcgaacgt taaaatcatg
    53461 tagccagacg gcataggttt tgcccgtttc ctcaccttta cgataggccg ggcgcgggtc
    53521 ctgcgccagt acttcgcgga taaacagcgt taactgcgga taacgcttct ccagcgtcaa
    53581 aagctgcttt tcgacctctg cggtaaaact caccgccatc tctgcagctg gcgcactttg
    53641 cgcatagctg gcactggcat cgggaagcga ttcggcaaag gggagatacg gtttgatatc
    53701 cactaccggc gtaccatcga ccagatccag actgccgagc ttcagaatca cgctgccttt
    53761 atggcaaaca acctctttca gctctaccag cgacatgcca attgggttag ggcggaaagt
    53821 agagcgtgtt gcgaaaaccc ccattctggc gttaccgccg aggcgcggtg gacgcacagt
    53881 cggacgccag ccgccttcca tcgtttgatg aaagacgaaa aggatccata aatggctgaa
    53941 tgcttccagg ccgcgaacgg cgtcggcctg gttgtaggaa gcaatgagat gcagttcgcc
    54001 gttggcgctt tttaccagac ctggctggcg cggaacggcg aatttttctt tatagggcga
    54061 gcgaataacg cctatttgct cgaactggaa actgctcatt tcgccgtaat gttaagcgca
    54121 gagccgatac atacagcctg acgatagcag cccggcgtac cgctggtgac ttcgcagcta
    54181 tgcagtaata cagcattggc tttcatttta gaggcattga tttgcatctg cttacgtgcg
    54241 gttggaatgc tcggcggaga gtcctgatta gaggcctggc aagagtcgcc actgacttca
    54301 ccgagatcgc ggaacggttt gccgactaat tcttctgcat tggtataaat tcggaccggc
    54361 gtggcgcgcg gcgctttcgg ttttgcaggc tccgctttcg gctggggtgc agtgctttga
    54421 acgggttcga caggggatct gcttaacatg gaacagccgc ttagcatgag tgctactaaa
    54481 cagatcggta aagcacgcat agtatttcct caatgtatga tcaaaacgtc aatattgaat
    54541 caggagcttg taaaaatgac aagacgggca agcgcccgtc ctgaatgata ttacaaattg
    54601 tggaaacagc ctaaaaatta ccagccttta acagcgccgc cgttaaacac tttgtttgct
    54661 gcttcgtaaa cttcatcaga ctgataagcc tggacgaatt tcttcacgtt ctcggcgtct
    54721 ttgttatctt cacgcgttac gatcaggttt acgtatgggg agtctttatc ttcgacaaag
    54781 ataccgtctt tcgccggagt caggccaatc tggctggcat aggtggtatt gataactgcc
    54841 agagcgattt gcgcgtcgtc cagagaacgc ggcagttgcg gtgcttccag ttcaacaatt
    54901 ttcagatttt ttgggttctc aacaacatca agaacggtcg gcagcaggcc aacgccatct
    54961 ttcagtttga tcaagcccac tttttgcagc agcagcagtg aacgaccaag attagttgga
    55021 tcgtttggca cggcaacctg cgaaccatcc tgcagttcat ccagtgattt gattttcttg
    55081 gagtaaccag caatcggata aacaaaagtg ttgcctactg cgatcagttt gtagccacga
    55141 tctttcagtt gctgatcaag gtacggttta tgctggaagg cgttggcgtc gatatcgcct
    55201 ttgctcaatg cttcgtttgg cagaacatag tcgttgaagg ttaccagctc aacgtccagg
    55261 ccatatttgt ctttcgcaac tttctgcgcg acttctgcta cctgctgttc ggcaccaaca
    55321 atcacgccga ctttaatgtg gtttggatct ttttcatcct gaccgcagcc taccagtgcc
    55381 agtgatccaa tcagggctcc cactgccgca aaggttttga atttgaacgc cataccttat
    55441 tccttcttca attatttatg ttgtgttgaa cgttacttgc gagtgacagc ccggacgatg
    55501 cggtcgcctg cgaactgaat taaataaacc agaatgacca gcaataccag taccgtattc
    55561 atcaccgtcg cgttgtagcc gatgtagcca tactgatagc caatctgacc taaaccaccg
    55621 gcaccgactg caccacccat cgcggaataa ccgaccaggg taatcagggt gatagttgcc
    55681 gcattcacca gacccggcaa cgcttccggt aacagcactt tacagacgat ctgcattggc
    55741 gtggcaccca ttgcgcggga agcttcaatt aacccggttg ggatctccag cagagcgttc
    55801 tcgaccatac gggcaataaa cggtgctgca ccaacggtta acggaacaat tgctgcctgc
    55861 aaaccaatcg atgtaccgac aataacgcgg gtaaacggaa tcatccatac cagcaagata
    55921 atgaacggga tggaacggaa aatgttcaca atcgcagaaa cggtacgata cagcttcgcg
    55981 ttagcaataa tttgccccgg acgcgtgaca taaagcagaa cgccaaccgg cagaccaatc
    56041 acaaacccaa aaaatccgga tacgaaggtc attgccagcg tttcccatac gccacgaacc
    56101 agcagccaca tcatcggctc agacataacc cagtacctct acttttacat ggtgttcctg
    56161 cagccaggca atggcggctt gcgtatcttg ttgtgtgccg tgcatttcag tcagcatgat
    56221 gccgaattta acgccaccgg cgtagtccat ctgcgcgcta ataatgttgt tgttgacgtt
    56281 gaaacgacgc gcggtttcag aaagcagtgg ggcatcgacc gattgaccgg taaactccag
    56341 acgcagcatc ggcacgcagt cagtaaatgg ctccgtttgc agacgttcct ggtaatcttc
    56401 cgggatatcc agatgcaggg tcgactgaat aaacttctgc gccagcggcg ttttcggatg
    56461 cgagaacact tcacttaccg tgtcctgctc gatcagttcg ccattgctga tgaccgccac
    56521 gcaatcacaa atacgcttca caacgtccat ttcgtgggtg atcaacagaa tcgtcaaacc
    56581 cagacggcgg ttaatgtctt tcagcagttc gagaatagaa cgtgtcgttg ccgggtccag
    56641 cgcgctggtg gcttcatcac acagcaatac tttgggattg ctggctaatg cacgggcaat
    56701 cgccacacgt tgtttctgcc caccggaaag attcgacggg tagctatcat gcttatcgcc
    56761 aagaccaacc aatgacagca attccgtcac gcgacgtttg atctcgtctt tcggtgtgtt
    56821 gtccagctcc agcggcagag ccacgttgcc aaaaacagta cgcgaagaga gcaggttaaa
    56881 atgctggaaa atcataccaa tctggcggcg agctttggtc aactcggatt ctgacagcgt
    56941 ggtcaattcc tggccatcga ccagcacgct accctcggtt gggcgctcca gcaggtttac
    57001 acaacgtata agcgtactct tacccgcgcc tgaggcaccg ataacgccat aaatttgtcc
    57061 agctggcaca tgcaggctga cgttgttcaa cgcctggatg gtgcgggtgc cctggtggaa
    57121 cactttggtg atattcgaaa gttttatcat tgattattta ttatcgtcat taagttagtc
    57181 gtagcatctc gaatgcctga aacgggcaac gccgtcaatg aaatggatgt taaggcatcc
    57241 agacgtctaa atcaatcagg tttatgcgaa gagcactttc ttgcaggtcg aaacatgcga
    57301 tactagcgtc ccatgcctta ttaaggagct ataaaaggtg gcgaagagcg tacccgcaat
    57361 ttttcttgac cgtgatggca ccattaatgt cgatcacggc tatgtccatg agatcgacaa
    57421 ctttgaattt atcgacggtg ttattgacgc catgcgcgag ctaaaaaaaa tgggctttgc
    57481 gctggtggta gtaaccaacc agtctggcat tgctcgcggt aaatttaccg aagcacagtt
    57541 tgaaacgctg accgagtgga tggactggtc gctggcggac cgagatgtcg atctggatgg
    57601 tatctattat tgcccgcatc atccgcaggg gagtgttgaa gagtttcgcc aggtctgcga
    57661 ttgccgcaaa ccacatccgg ggatgctttt gtcagcacgc gattatttgc atattgatat
    57721 ggccgcttct tatatggtgg gcgataaatt agaagatatg caggcagcgg ttgcggcgaa
    57781 cgtgggaaca aaagtgctgg tgcgtacggg taaacctatt acacctgaag cagaaaacgc
    57841 ggcagattgg gtgttaaata gcctggcaga cctgccgcaa gcgataaaaa agcagcaaaa
    57901 accggcgcaa tgattaaaag atgaacggtt gaaataaaaa tgcatttttc cgcttgtctt
    57961 cctgagccga ctccctataa tgcgcctcca tcgacacggc ggatgtgaat cacttcacac
    58021 aaacagccgg ttcggttgaa gagaaaaaat cctgaaattc agggttgact ctgaaagagg
    58081 aaagtgtgat atacgccacc tcgcgacagt gagctgaaag ctgcgtcgca actgctcttt
    58141 aacaatttat cagacaatct gtgtgggcac tcgaagatac ggattcttaa cgtcgcaaga
    58201 cgataaatga ataccaagtc tcaagagtga acacgtaatt cattacgaag tttaattctt
    58261 tgagcatcaa acttttaaat tgaagagttt gatcatggct cagattgaac gctggcggca
    58321 ggcctaacac atgcaagtcg aacggtaaca ggaagcgctt gctgcttcgc tgacgagtgg
    58381 cggacgggtg agtaatgtct gggaaactgc ctgatggagg gggataacta ctggaaacgg
    58441 tagctaatac cgcataacgt cgcaagacca aagaggggga ccttcgggcc tcttgccatc
    58501 ggatgtgccc agatgggatt agcttgttgg tggggtaacg gctcacc
//
LOCUS       DAOFMX010000016        58059 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.016, whole genome shotgun sequence.
ACCESSION   DAOFMX010000016 DAOFMX010000000
VERSION     DAOFMX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.016"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(24..278)
                     /locus_tag="QBA20_001118"
     CDS             complement(24..278)
                     /locus_tag="QBA20_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDQ6291743.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="QBA20_001119"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="QBA20_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDQ6291744.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="QBA20_001120"
     CDS             1228..2694
                     /locus_tag="QBA20_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDQ6291745.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="QBA20_001121"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="QBA20_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDQ6291746.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="QBA20_001122"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="QBA20_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDQ6291747.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="QBA20_001123"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="QBA20_001123"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDQ6291748.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="QBA20_001124"
     CDS             5709..7265
                     /locus_tag="QBA20_001124"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDQ6291749.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="QBA20_001125"
     CDS             7347..9161
                     /locus_tag="QBA20_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDQ6291750.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGHITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HILQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="QBA20_001126"
     CDS             9162..10256
                     /locus_tag="QBA20_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDQ6291751.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="QBA20_001127"
     CDS             10256..11281
                     /locus_tag="QBA20_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6291752.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="QBA20_001128"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="QBA20_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDQ6291753.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHIVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="QBA20_001129"
     CDS             complement(12876..13220)
                     /locus_tag="QBA20_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291754.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="QBA20_001130"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="QBA20_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDQ6291755.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="QBA20_001131"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="QBA20_001131"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDQ6291756.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="QBA20_001132"
     CDS             15616..17376
                     /locus_tag="QBA20_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDQ6291757.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCHE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="QBA20_001133"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="QBA20_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDQ6291758.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="QBA20_001134"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="QBA20_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDQ6291759.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="QBA20_001135"
     CDS             19113..19340
                     /locus_tag="QBA20_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDQ6291760.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="QBA20_001136"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="QBA20_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDQ6291761.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="QBA20_001137"
     tRNA            21195..21271
                     /locus_tag="QBA20_001137"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="QBA20_001138"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="QBA20_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HDQ6291762.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="QBA20_001139"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="QBA20_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDQ6291763.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="QBA20_001140"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="QBA20_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDQ6291764.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="QBA20_001141"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="QBA20_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDQ6291765.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="QBA20_001142"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="QBA20_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDQ6291766.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="QBA20_001143"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="QBA20_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDQ6291767.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="QBA20_001144"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="QBA20_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDQ6291768.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="QBA20_001145"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="QBA20_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDQ6291769.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="QBA20_001146"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="QBA20_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDQ6291770.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="QBA20_001147"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="QBA20_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDQ6291771.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="QBA20_001148"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="QBA20_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDQ6291772.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="QBA20_001149"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="QBA20_001149"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDQ6291773.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="QBA20_001150"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="QBA20_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDQ6291774.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="QBA20_001151"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="QBA20_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDQ6291775.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="QBA20_001152"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="QBA20_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HDQ6291776.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="QBA20_001153"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="QBA20_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDQ6291777.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="QBA20_001154"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="QBA20_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDQ6291778.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="QBA20_001155"
     CDS             37391..37555
                     /locus_tag="QBA20_001155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291779.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="QBA20_001156"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="QBA20_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDQ6291780.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38328..39359)
                     /locus_tag="QBA20_001157"
     CDS             complement(38328..39359)
                     /locus_tag="QBA20_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HDQ6291781.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39593..39952)
                     /locus_tag="QBA20_001158"
                     /pseudo
     CDS             complement(39593..39952)
                     /locus_tag="QBA20_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40170..41813)
                     /gene="yojI"
                     /locus_tag="QBA20_001159"
     CDS             complement(40170..41813)
                     /gene="yojI"
                     /locus_tag="QBA20_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDQ6291782.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41889..42539)
                     /gene="alkB"
                     /locus_tag="QBA20_001160"
     CDS             complement(41889..42539)
                     /gene="alkB"
                     /locus_tag="QBA20_001160"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDQ6291783.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42539..43603)
                     /gene="ada"
                     /locus_tag="QBA20_001161"
     CDS             complement(42539..43603)
                     /gene="ada"
                     /locus_tag="QBA20_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDQ6291784.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43677..44732)
                     /gene="apbE"
                     /locus_tag="QBA20_001162"
     CDS             complement(43677..44732)
                     /gene="apbE"
                     /locus_tag="QBA20_001162"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDQ6291785.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44844..45965)
                     /gene="ompC"
                     /locus_tag="QBA20_001163"
     CDS             complement(44844..45965)
                     /gene="ompC"
                     /locus_tag="QBA20_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDQ6291786.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46703..49375
                     /gene="rcsD"
                     /locus_tag="QBA20_001164"
     CDS             46703..49375
                     /gene="rcsD"
                     /locus_tag="QBA20_001164"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDQ6291787.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49392..50042
                     /gene="rcsB"
                     /locus_tag="QBA20_001165"
     CDS             49392..50042
                     /gene="rcsB"
                     /locus_tag="QBA20_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDQ6291788.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50242..53091)
                     /gene="rcsC"
                     /locus_tag="QBA20_001166"
     CDS             complement(50242..53091)
                     /gene="rcsC"
                     /locus_tag="QBA20_001166"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDQ6291789.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53366..54142)
                     /locus_tag="QBA20_001167"
     CDS             complement(53366..54142)
                     /locus_tag="QBA20_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDQ6291790.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54147..55796)
                     /locus_tag="QBA20_001168"
     CDS             complement(54147..55796)
                     /locus_tag="QBA20_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDQ6291791.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55797..56356)
                     /locus_tag="QBA20_001169"
                     /pseudo
     CDS             complement(55797..56356)
                     /locus_tag="QBA20_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56515..>58059)
                     /locus_tag="QBA20_001170"
     CDS             complement(56515..>58059)
                     /locus_tag="QBA20_001170"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDQ6291792.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14198 a        15664 c        14267 g        13930 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca tataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 tcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacattgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc acgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacacct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag tgagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatccg gcataccatg ccggatgtgg cacatcatta caacgcaata
    38341 tccgccactt ctttttgaac gggttgcggt ttcaactgcg gtttcggcgt actatctgct
    38401 gcttgcggtt tgtcgtattt cagccccagc acttcgctgg tgtactgcaa ctcgcgttct
    38461 gttgccgcta catcaccgtt cagcttgcgt ccataagacg gaacgatcgc tttcaacgta
    38521 gcctgccatt gcgggctgga aacgcgatcg ccaaatactt tttccagtag attcaacata
    38581 atcggcgcgg cggttgacgc ccctggcgat gcccccagca gcgccgcaat ggtcccttgc
    38641 tggtcactga ccacttcggt acccagacgc agtacgccac ctttctcggc atcacgcttg
    38701 ataatctgca cacgctgccc cgcttgccac aaacgccagt cctctttttt cgcttgcgga
    38761 tagtactctt tcaacgcttc aaaacgatcc tcttcactca acatcacctg actcaccaga
    38821 tatttcacca gatcgaaatt atccagcccg acgtgcatca tcggcatcac gttagaggtg
    38881 gtggtggaac tcatcagatc ccacaatgaa ccgtttttga ggaatttggt tgagaaggtg
    38941 gcaaatggcc caaacagcac tacgcgttta ccgtccagaa cacgggtatc gatatgcgga
    39001 accgacatcg gtggtgcgcc aacggatgct ttaccgtaaa ctttcgccag atggcgatta
    39061 accacgtctg gattttccga aacaaggaac tgtccgccca ccgggaaacc ggcgtagtct
    39121 ttcgcttccg gaatccccga ttcctgtaac agcttcagcg ccgcaccgcc cgcgccgata
    39181 aagacaaatt tggcgcggat gttctgtgca gtgccgtttt tcagatcggc aacggtaacg
    39241 gtccaggtgt tatcgtcatt acgctttagg gcgcggactt cgctgctgag ttgcagcgag
    39301 aagttagatt tcttctgtaa ggaagcaatt aactggcggg tgatctcacc atagttcaca
    39361 tcagtgccaa tttctgttgc ggatcgcgcc cttccatcac taacggtgcc cactctttga
    39421 tctgcgcgtg atcttcagag taacgcatac cgcgaaacag cgagctttgt tgcaacgcgg
    39481 cgtaacgggc gcgcaggaaa ttaacgttat cctcgcccca gacaaagctc atatgcggaa
    39541 cggtattgat aaatgaacgc ggagtacgca gcacgccgcg ctcgacctgg tgcgcccaga
    39601 actggcggga aatctgaaat gcttcgttaa tggcgactgc tttttcaata ctgatgctgc
    39661 catcggcgtt ttgcggggtg tagttcagtt ccatcagtgc agaatgcccg gttccggcgt
    39721 tgttctagcc gttcgaactc tcctgcgcgc ccccctccag gcgctccacc atggtcatcg
    39781 accattcagg ctccagctcg cgtcaatagg tccccaacgt ggcgctcata atgccgccgc
    39841 caatcaacag tacatcagtt tcctgctcct cggacgcttt cgcttttgcc gccatcgaaa
    39901 cggcattaag ccccacggcc atcgagaaga gcatggcagt cacttttttc atcttgttaa
    39961 tgccttactt ttagtcgctt tattgcaggt gagatttgcg cggcatcaac ggtaacactt
    40021 aagtaacaac atttaaataa tgtttataaa ttatattcta attttagaaa cattataatt
    40081 tcgttgatta attatagggt tattagaaat gaagggattt ttagcatatt gacctgacgg
    40141 cagcagtccg ccaggtcagc gtcgtaatat tatgccgtcc gggcaacggc atcacgcgaa
    40201 gcggcatcgc gctcttcgcc cgtcagctcg ctaagttgcc cattgcgcat ttccagcagg
    40261 cggtcggcgt ggataaagta atgatcatca tgactgatag cgaaaatagt tttacccatc
    40321 tcctgcatca gcggcagcaa cacctgataa aactcacgac ggaagtgtgg atcctgatcc
    40381 accgcccatt catccagcag gataatatcg cgttcttctg ccagcgccag caacagcgcc
    40441 acgcgttttt tctgcccttt tgataacttc aggttaacaa tacgcccgtt gcttaactca
    40501 agcttatgag ccattttcag ctgcgccagc cacttctcaa ccagttgcgg gttagcgggt
    40561 tgcccctccg gccccagcag ttgatcaaac agccagacat cggtaaacac tgccgaaaac
    40621 agtttgcgat aatcttccgg ttgttcgccg ctgacaggtt tgccatccag caagatttcg
    40681 ccgctttgtg gctgatacaa gcccgtcaac aacatcgcca gcgtcgattt tccgctaccg
    40741 ttaccgccaa tcagaaacag cagctcgcca cgtttgatgg tgagattaat cggaccaacg
    40801 gaaaacgcgt tatcctgata agcaaacgtc acgttacgca gctccagcgt ttgccagttg
    40861 ggaaacgcct gcgggcgcgg aaactcagct ttgaaaggcg cgagcgcgaa tttgttcagc
    40921 ttgttaaacg ccacctgcgc cgtcagcagc gtcggcaatg cgccaaccgc cgaaagcagc
    40981 ggcgtacgca ggaataacag cgtcaacgaa taggtcgcag caacgttggt atcagcccaa
    41041 ccgagactgt tcgccatcca gaacaccagg ccgatagcgc ccagcatcat gatgtttgac
    41101 cagttcacgg cactaagatg gaaggtgtct gcgcgaataa tatgatggcg atactcttgc
    41161 gcatcgggga tgtagaggtt gttaaacaca tactcggcgc gttcccggtt cagagtcagc
    41221 tctttgcgcc cttcaagtac agtttgaaaa tccgtgtaca gcttgtcttc ggtttcacgt
    41281 agggtcgcca tatgtttgta cacccgcgcc accagcacaa aaccgcccca gatggtgatc
    41341 gccatccaga tagccgttac cagcaacatt ttgcccgaca gcatccacag atacgccgat
    41401 gaaccgatag tgagaatgat cccctgcccc agttccggca gacgcacaaa agcaatggtg
    41461 atattgcgca catcgctggt taatcccgcc agcaacgagg cgctaccgag ttgttcaatg
    41521 cgctcgacgt gggtatccag aatccgcttg ataaattcac tacgcagtcg gtaaacgaag
    41581 tgatgcccca aagtggtgag cgccagttgc gatccgagag tgactgccat caacagcagc
    41641 aataatccca gaaactccgg taacaccagc agactggtat ccgccgtttc gataaggcgc
    41701 tgattgataa aagcaattaa gccaatgcct aatgccgcac tggcgaggct tagcgccatc
    41761 acactgataa atggccagcg atactgccgc cagacaagta caagaagttc catgcagaaa
    41821 acccggacaa tgaattacag cccgcagttt aaacatcttc gcgcgcacag caataataat
    41881 tcttattttt attctttttt acctgcctga cggaatgtca ggttgtagcg gcagtcggtg
    41941 gtgagtggat gaaaccccgc tttcaacggt tgaataccgt gataaaacag ccgcgattca
    42001 ccgccccata ccaccacatc gccatgttcc aacaacaaac gtttgagcgg atcattacgt
    42061 ttcaggccac caaattgaaa aatcgcgggt aagcccagag aaacagaaac aattggcgcg
    42121 cgcagatccg gttcgtcttt atcctgatgc agcgacagtt tcgcgccagg agcgtagcgg
    42181 ttgataagac aagcatctgg ctggaaatct ggatagcccg ccgccgtagc cgcacgttga
    42241 cataaatcat gaaagctttg tggcatggcg ggccacggtt tatttgtttg cggatcaatg
    42301 ggcgaataga gataaccttg ccgatgggtc gtccagccca gatgcccaca gttggtcatc
    42361 gccaccgaca tggtatatcc gcctggagcg accatctggc gaaacggcga ctggctggca
    42421 acgtcattaa tatcgcggat tagttgctcc gcagcattaa aagcaaaacg ccgtaaaatt
    42481 accgcaccag ccgccagtgg ctcttgccac ggttcagcat cggcaaacag atccaacatt
    42541 acctctcctc attttcagct tcgcggcgca gcagttgcgc tttacgcgac acgccccagc
    42601 ggtaaccgga aagtgtgcca tcaccacgga ccactcgatg acagggtatt acgatagcca
    42661 gcttgttggc ggcacaggcg ctggcaacgg cccgtaccgc tttcggtttg ccgatggcgt
    42721 tagccagttg ctgataactg acggtttcac cacaaggtat cgtgcgcagt gcctgccaga
    42781 cttgttgctg aaaagcagtg ccgcgaatat ccagcggtaa cgtcagcggc gtatcgcgtt
    42841 gattgaggct ggcgatcact tcacgcacat gttgctgaaa cgtcagatcg gcaggcgcgt
    42901 tgtcggcagc gggaaacatc tgctgcaact cgctgattaa tgtcgcgtca tcatcgccca
    42961 gcaatatcgc gcaaatcccc cgctcgcttt ctgccaccag gcaacgaccc agctcacaat
    43021 cagccagcgc gtaacgcacc gccagatttt cgccaccgtg acggaattgt ttagccgtca
    43081 tgcccagcgt ttcatcggct ttgcggtagt aactgctgct gtcggggaat ccggcgttaa
    43141 gaatagacgt cgtcacgctc tcccctttcg ccagcgattc gcgcaaacgg cgagcgcgcc
    43201 aggcctgttg ccaggcttta ggcgtcattc cggtagtagc tttaaacaac cgatgtagat
    43261 gaaatggact catcgccacc tggtcggcta aggcttccag cgttacaggc gtttcctgtt
    43321 ccagcagtcg acacgcgtgg gtgattttat ccaaccgatg ttgccgggga ttggctttgt
    43381 ctggctgaca acgtttgcag gggcgaaatc cggcggcgag tgcctcgctg gcatttgcgt
    43441 agaaggagac attttcccgc aaggcatgtc tggcgcggca agaaggacgg caaaagatac
    43501 ccgtggtgcg cacggcgaaa acgaattcgc cgtcggcatt cgggtcgcgg gctaagacag
    43561 attgccagcg ttgatcgtca gttaagcatg tggcgttttt cataatcagc tccctggtta
    43621 aggatagcct ttagtctgcc cggtcaccat cacgcaaaaa ccaacaatct tgcgctttaa
    43681 tttttttcgc tgacaaggaa gcttttaaac tgtggtgaca tccaggtttt aaagctatcg
    43741 ccttctttgg tgatcatata aaccgccagc ccttcccggc gaacaacctc tttggctttc
    43801 tccggcccga gtaccatcaa gccagtatcc caggcatcgg cttccagcgc cgtcggagca
    43861 atcaccgtca cggataccag attgtgttcg atgggacgcc cggtttgcgg atcgataaca
    43921 tgggaaagac gtttgccgtc cagttcgtaa tagttacggt agctgccaga ggtgctaatc
    43981 ccatgaccgt tgatatccac cacagcctga accgcgtttt ctttatcggt tggtttttga
    44041 atcgctaccc gccacggctg gccttcaccg ttcataccac ggctgttcag cgcgccgccc
    44101 accgacacca gatagcggga aatcccttcc tgctccatca agcgtgccag gtgatccgcc
    44161 gcataacctt caccgacggt ggagagatcg acatataaat ccggcaggtc tttttgcaga
    44221 tattgctgat gcgactgatt gatgaccgtc aggtgctgta agccggtttt ggctttcatc
    44281 gcatcgatct gttcctggct cggaatttga accggctgtt gttccgggcc aaagccccac
    44341 agattcacca gcggcccgac ggtaatatcc atcgcaccat cggtccttgc cccaatgcgc
    44401 agcgaggtgg tgacgatatc ggccatcgct tcacttaccg gccacggcga caaactttgc
    44461 gagtcgttaa agcgcatcag cgcggaatct tttttatagg tcgaaagcag ctgatcgtcg
    44521 gcgtccagct gggtctgaat cttttcttta agttcggcac tgcgtttggc atcaatgccc
    44581 gggatgctgg cacgccagaa ggtacccatg gttttgcctt caagaacggt aacttcagta
    44641 gcgtgggttt tggcgggttg tggtttttga tcgcaaccaa caaagaagag cgcggcaacc
    44701 agcagtgcca cgcgggtaaa gcttatttcc atacgtgatt atcctcatgc gaacggtcgc
    44761 aagagtacac caaaaaactg tgtttgtacg ctgaaaacaa tgaaaaaagg gcccgcaggc
    44821 cctttgttcg atatccatcg agattagaac tggtaaacca gacccagagc tacgatgtta
    44881 tcagtgttga tgccagcgtc acgagtgaac tggttgtcgt ccagcaggtt gattttgtag
    44941 tcaacgtagg tggacatgtt tttgttgaag tagtaggtcg caccaacatc aacatatttc
    45001 aggatatctt cgtcgtcgta gttacgacca ttgatgacac ccaggttttt acctttagac
    45061 tgcaggtaag ccagggacgg acgcagaccg aagtcgaact ggtactgagc aacagcttcg
    45121 aagttctgtg ctttgttcgc ccaacccaag ttgcctacgc gagttgcgtt gtaggtctgg
    45181 gtgtactgag cagctaggta gatgttgttc gcgtcgtatt tcagaccacc ggtgtaggtt
    45241 tcagcgcggt cgccattacc gatgtaacgc tcatcataac ggttcagacc gaagttctgg
    45301 tcatcagtac gtttggagct ggaaactgca gcaccgatac cgaagccttc gtaatcataa
    45361 gtaatagaac cgccaacgcc gtcaccgttc tgacgcagag cttcacgacc gttgttggtc
    45421 atgccttcac cttccgggct accgtttttg ccctgatact gaacagcgaa gttcagaccg
    45481 tcaaccagac caaagaagtc ggtgctacgg taggttgcga agccgttacc acgctgctgc
    45541 atgaagttgt cagaaccgta agtgtcacca ccgaattctg gcagtacgtc ggtccaggaa
    45601 gttacgtcgt aaactacgcc gtagttacga ccgtagtcga aagaacctac atcctggaat
    45661 ttcagacctg cgaatgccac acgggtccag gagttgtttt cgttttcagc gctgttgccc
    45721 tggatctgat attcccactg gccgtaaccg gtcagctggt cagtaacctg agtttcacct
    45781 ttgaagccaa gacgcatgta ggtctggtcg ccatctacag acttgtcgtc agagaaatag
    45841 tgcaggccgt ctactttacc gtacagatct aatttgttgc cgtctttgtt gtaaacttca
    45901 gcagcgtttg ctgcgcctgc taccagcaga gctgggacca ggagggacag tactttaact
    45961 ttcatgttat taaccctctg ttatatgcct ttatttgctt ttttatgcca ctgcatactg
    46021 attaaccctc attaatcagt cggcaagtcc attctcccca aaaatgcaga ataatccaac
    46081 acgaatatga tactaaaact tttaagatgt ttcatttatc gctatagatg tttcaaaatg
    46141 taaatacaag ggaacttttt aagattattg cggaatggcg aaataagcac ctaacatcaa
    46201 gcaataataa ttcaaggtta aaatcaataa cttattctta agtatttgac agcactgaat
    46261 gtcaaaacaa aaccttcact cgcaactaga ataactcccg ctatcatcat taactttatt
    46321 tattaccgtc attcatttct gaatgtctgt ttacccctat ttcaaccgga tgcctcgcat
    46381 tcggtttttt tacccttctt tacacacttt tcattattct gtgctaccac agaaaaacta
    46441 taacgcttgt taactatttc acaaataatt aacatcagca taatttccag caatctttgt
    46501 ttatttgcaa ttaatttcgt tgggcttttt gtaggttatt tgtacagcaa aatggcgctt
    46561 gtacatctat ttccccccat acaggatgat aaatatcacg ggagaataga gaatcatcaa
    46621 tcaggtaaga gtctggaatt tcacactgta ccctttatac tgccctatca cttcgcgaag
    46681 ttttaacagg tcataaacac gaatgcgtca gaaagagaca acggccacga cccgcttttc
    46741 actcctaccg gggagcatta cccgcttctt tttactgttg atcattgtgt tactggtgac
    46801 gatgggggta atggtacaaa gtgccgttaa cgcttggctg aaagataaaa gttaccagat
    46861 tgtcgacatt acccacgcaa tccaaaagcg cgtcgatacc tggcgttacg tgaccaggca
    46921 gatctacgac aacattgccg cgacgacctc cccctcctcc ggcgaaggtt tacaagagac
    46981 gcgcctgaaa caggatgtct actatctgga gaaaccacgc cgcaaaacgg aagcgttaat
    47041 ctttggctct cacgacaact caacgcttga gatgactcag cggatgtcca cttatctgga
    47101 tacattgtgg ggtgcagaaa atgtaccgtg gtcgatgtat tacctgaatg gtcaggataa
    47161 cagtctggtg ctgatctcaa ccctacccct taaagatctc acctccggat ttaaagaatc
    47221 gaccgtcagt gacattgttg attcacgtcg tgcagaaatg ttacaacagg ccaacgccct
    47281 cgatgaacgc gaaagttttt ctaacatgcg ccgcctagcc tggcagaacg gtcattactt
    47341 taccttgcgt accacgttca accagccggg acatctggca acggtcgtgg cttttgatct
    47401 gccgattaat gatttgatcc caccgggtat gccgctggac agtttccgcc ttgagccaga
    47461 cgcgacggca acgggaaaca atgataatga gaaagaaggg acggatagcg tcagtatcca
    47521 ctttaacagt acgaagattg aaatctcctc ggcactcaac tctaccgata tgcgcctggt
    47581 ctggcaggtt ccttatggca ccttattgct ggatacgttg caaaacattc tgctgccact
    47641 gctgctgaac atcggtttgc tggcgctggc gttatttggc tataccacat tccgccattt
    47701 ctccagccgc agtacagaaa gcgtccccag cacggcggtc aataacgaat tgcgcatttt
    47761 acgggcaatc aatgaagaga tagtctcact gttgccgctc ggcctgctgg ttcacgatca
    47821 ggaatcgaac cgcactgtca taagtaacaa aattgccgat catttgctgc cgcatctgaa
    47881 tctgcaaaac atcaccacca tggcggaaca gcatcagggg attattcagg cgacgatcaa
    47941 taacgagctg tatgagatcc gcatgttccg cagccaggtc gcgccgcgca cacaaatttt
    48001 cattattcgc gatcaggatc gcgaagtgct ggtaaacaag aaactcaagc aggcgcagcg
    48061 tctgtatgag aaaaaccagc aggggcggat gacctttatg aaaaacattg gcgatgcgct
    48121 gaaagaaccc gcacagtccc tggcggagag tgcggctaaa ctcaacgccc cggaaagcaa
    48181 acaactggcg aatcaggcag atgtgctggt gcggctggtc gaagaaatac agttagcgaa
    48241 catgcttgcg gatgatagct ggaaaagtga gacggtgctg ttctccgtgc aggatttaat
    48301 tgatgaagtt gtgccttcag tgttgcctgc catcaagcgt aaaggtctgc aactgctgat
    48361 taacaatcat ctgaaagcac acgatatgcg ccgcggcgat cgcgatgcct tacgacgtat
    48421 tttgctgcta ctgatgcaat atgccgtgac ctcaacgcaa ttgggaaaaa tcacccttga
    48481 ggttgatcag gatgagtcct ctgaagaccg cctgacattc cgcattctgg acacgggaga
    48541 aggcgtaagt attcatgaaa tggataattt gcacttcccg tttatcaacc agacccaaaa
    48601 cgatcgttat ggaaaggcgg acccgctggc attctggctg agcgatcaac tggcacgtaa
    48661 actgggcggt catttaaaca tcaaaacgcg ggatgggctt ggtacacgct actctgtgca
    48721 tatcaaaatg ctcgccgctg acccggaagt tgaagaggaa gaagagcgtt tactggatga
    48781 tgtctgcgta atggtggatg ttacttcggc agaaattcgg aatattgtca ctcgcctgtt
    48841 agaaaattgg ggtgcaacct gtatcacacc cgatgaaaga ttaattagtc aagattatga
    48901 tatcttttta acggataatc cgtctaatct tactgcctct ggcttgcttt taagcgatga
    48961 tgagtctggc gtacgagaaa ttgggcctgg tcaattgtgc gtcaacttca atatgagcaa
    49021 cgctatgcag gaagcggtct tacaattaat tgaagtgcaa ctggcacagg aagaggtgac
    49081 agaatcgcct ctgggcggag atgaaaatgc gcaactccat gccagcggct attatgcgct
    49141 ctttgtagac acagtaccgg atgatgttaa gaggctgtat actgaagcag caaccagtga
    49201 ctttgctgcg ttagcccaaa cggctcatcg tcttaaaggc gtatttgcca tgctaaatct
    49261 ggtacccggc aagcagttat gtgaaacgct ggaacatctg attcgtgaga aggatgttcc
    49321 aggaatagaa aaatacatca gcgacattga cagttatgtc aagagcttgc tgtagcaagg
    49381 tagcctatta catgaacaat atgaacgtaa ttattgccga tgaccatccg atagtcttgt
    49441 tcggtattcg caaatcactt gagcaaattg agtgggtgaa tgttgtcggc gaatttgaag
    49501 actctacagc actgatcaac aacctgccga aactggatgc gcatgtgttg attaccgatc
    49561 tctccatgcc tggcgataag tacggcgatg gcattacctt aatcaagtac atcaagcgcc
    49621 atttcccaag cctgtcgatc attgttctga ctatgaacaa caacccggcg attcttagtg
    49681 cggtattgga tctggatatc gaagggatcg tgctgaaaca aggtgcaccg accgatctgc
    49741 cgaaagctct cgccgcgctg cagaaaggga agaaatttac cccggaaagc gtttctcgcc
    49801 tgttggaaaa aatcagtgct ggtggttacg gtgacaagcg tctctcgcca aaagagagtg
    49861 aagttctgcg cctgtttgcg gaaggcttcc tagtgaccga gatcgctaaa aagctgaacc
    49921 gcagtattaa aaccatcagt agccagaaga aatctgcgat gatgaagctg ggcgtcgaga
    49981 acgatatcgc cctgctgaat tatctctctt ctgtgacctt aagtccggca gataaagact
    50041 aatcatctgt aggccagata agacgcgtta gtgtcttatc tggcatttgc accgattgcc
    50101 ggatgcggcg taaacgcctt atccggccta cgattcccat tatttcaaca gattacatta
    50161 aagtaggcca gataagacgc gtcagtgtct tatctggcat ttgcactgaa tgccggatgc
    50221 ggcgtaaacg ccttatccgt cctacgaatc ccgcgatttc ctgaccctct cggcatatac
    50281 cgtcagcgtc tgttttatca catccagcgt taccggcttc gagaggcagc tgtccatacc
    50341 ggactccaga caccgctgct tctcttcagc caacgcatta gcagttactc cgattaccgg
    50401 caacgtcagt cccaactgac gaatgcgttg cgtcaagcgg taaccatcca tatttggcat
    50461 gttgacgtcg ctaagcacga tatcaatatg attcttacta agtacattaa gcgcatcgac
    50521 gccatcattc gcggttttac attgatagcc caacgatccc aactgatctg ccagcagacg
    50581 tcggttaatc ggatgatcat ccacgaccag aatcatcata tcgtcgttat cgctgaccgc
    50641 tttgtccgtc gacggcagag cgttagcagg atcgtcgctc tccatctcga tcaaataaat
    50701 acgcgccaac aatgccggta gctcatgcgg agcagccaca ctgtgtaccc actcccctgg
    50761 cgctttctcc agcggaatac caatatggcg acgacagaag gtcactaccg ctctgccctg
    50821 ccatttttta ctcactacct cgtcagtgat taacacatct tcgggagtcg gttcctgccc
    50881 ttcgtatgtt gtaacgacga tgccgctgcg ctgcaaactg gtttccagga actgacagag
    50941 cgacgcattg cggaccgcca gccagcagcg tttaccactc aacccttcca cgcctttttt
    51001 ctgcgggtac tgagcgccgt acaacggaat acgcacggta aactggctgc ccattcccgg
    51061 ttctgaatct accgagatat cgccgtccat catgctgatc agtttttcac aaatcgccag
    51121 acccagaccg gtcccctgga agtttcgctg tacgcccgtt ccaacctgga agaagggatc
    51181 aaacaagcgc accacttctt tcgccggaat ccccacgccg gtatcgcgaa cacggataga
    51241 gagataatcg ccatcagcgc gaacatgcaa aactatacag ccggtatcgg tgaattttat
    51301 ggcgttactc aacaggttgg agatgacctg ctgtaaacgc atcgggtcgc catttaaggc
    51361 cactggcaca tccggttcaa taaagcagta caagcctaac tgcttgcgta ccaccagcgg
    51421 taaatagttg gcggtgatgt ggttcatcac ttcacgcggt gaaaactcac gcggttcgat
    51481 cttcaactgt tccgattcaa tcttcgagaa atcgagaata tcgctgataa ttttcaacaa
    51541 caggctggaa gagttgttca ttgccgtcac cagacgatcg acgcctttcg gtaactcttt
    51601 ggtttgcaac agatccaggt taccgataat gccatacagc ggcgttcgca gctcatgact
    51661 gacggtggca aggaacatcg attttgactg gctcgcctgt tccgctgctt gtgccatctc
    51721 ctgcaacgac tcttccatct tcacgcgcga agaaacatcc accagcacac aaatggccac
    51781 gttttcatta cgatagcgcg aatggacgaa gctgatttgc agattggtat tgttgctggt
    51841 caggacatca acaaaattga cctgctgccc acagataatt tgcgtcagtc gttggcggtc
    51901 ctcatgcgta agcatattga gataggtatg cgccagttcg ttacttaaaa tattgacgcc
    51961 atcagcggta cgcaaaatgc agatacccac tggcgcggag gcgacaatct tgcgattgaa
    52021 ctgctcatgt tcttccagtc gcagggcgtc gctttccgcc ggaatgaaaa tacgtcgctc
    52081 gtacatccgc gcgagagtaa acaatgcagc tccggcaagc acattcagca aaattgcgtt
    52141 aaggatcaac atgcgaatgc gttccagcac cttatcaacc ggcaccgaat acacgatgct
    52201 tagcgatgag ggtggcagat ttttcttcag caccagctcc cggaaccctt ccgtatagcc
    52261 aaaccaggag cgttcctgca tccagcgagg atcgccctta attttacttt ctggtccggt
    52321 aagcgaaatc agggtatgac cattttcatc aagaatggta acccccatcg gcaacgtacc
    52381 cggtaagaaa aagttctcca tccggatggt ctgctcgaca cccaaaagcg cctgcaaccg
    52441 gttcgccaga taaactggcg tcaacgcgta aaaatacccg acgcaagggc gcggaccttc
    52501 gctgatccag tagaggttac tgccgctatc atcttgtggt gcatttcgat atttattgat
    52561 gcgttcatgc aacgctttca acgcggtatc gcgttccact ggcatatcac gcagaccgaa
    52621 attggccatg cagaggttat cactgccgat taaaaatacc cggttgagat cgtaagccgc
    52681 agaaaaatta tcgcgccagt agcgcataaa ccacgccaat gactccagag aacctcgcca
    52741 ggtgttactc attgcggaac aatcggagtc ggcaaacagc ggttcaaacg caggcacatc
    52801 cgcctgcgtt tctcgtccac gcggggaaag cacaccgttt tctgccgata agcgattttc
    52861 ggcgatgtac ttaagctctt tcatcacatc agaggtgcgt tgaataaagc gctgagcctg
    52921 atcggaactc agattaaatt cctgacgaat ttccgattct cgctgatgta acgcattaac
    52981 gatgtaaaaa acggatgaaa aagcaatcaa cagccagagc actaacgcca atgctctgaa
    53041 catgtagcgc gaggctttca gggttgtacg aaaagaagca aggtatttca aaggggagaa
    53101 gctccgcctc aggtgaccga tggagtgtgg ttaaggtagc ggtaaaagcg tgttaccgca
    53161 atgttctgtc accattgcaa taatgcgtat cgcttagaaa ccattgcctt aaccaggaaa
    53221 gccggagtag atggacagtt ctcagtacga aaaataagaa atgaccaatc tgccgacgct
    53281 tgttaatggt tatatcctgg cgtcggcaga tcgcccagca gtcttatctg aaaaaagaga
    53341 gagccaggtt gtgtgaaggg atggatcacc agtcaaaact tttcaccagc gtcagctcgc
    53401 cagcattacg catcggtaca ataaatgttt cctgtttctc attgaccgat ccttcatcgg
    53461 tgatcagcgt cagttgggcg gtcgtcaatt ccgtttcgct gcgcccacca tagtagttga
    53521 tatacacctg atagcgcccg tgaactggtg ctggcatggc gaaaatctcg ggtccgtacc
    53581 ccgtcgtgac atccatatcc agtgcaccac tgtttttcag cacggtgtta ccgtaccagg
    53641 cgtgttcgcc atcgggcgtg acaacatgga gatcgagatc ggtattgtcc gtatcccacg
    53701 agagaaccag ccgtaaacgt gcacgaatag ttcctgtacc cggcgttgag taaaactgca
    53761 ttttttgccg gctttgccca tccgggctga tgacctgcac gctgttgctg ccttcagtga
    53821 aaatataagg gcgtgcaaat gaaccatcgg attcaattct ctgcggcata ctggcaccgt
    53881 tgaccaccag ccggccctgc tgaactttac ccgccgcagc ataattcttt attttgccgc
    53941 ggatctgcgc tgagatattt tgatcatctg ccatgttgac ggacgacgcc gggtaattga
    54001 tcgattgtga aaaactggca tcttcgccct ctgccggatg ccagccagag agcggtgcgt
    54061 caatctcgac ttcctgcaca ccttcactgt gagcgacagg cgaaagtgcc accagtaaca
    54121 acggaagaaa aatctttcgc atagcattag tccattaata attgtcgggt cagcgtttcg
    54181 atatagttct catcaagccc ggtgggataa ccatcaaagg ccaagtgcag atactcatgg
    54241 gttaaatcca gccgatcctg aagcgtaaag aagttacgaa taaacagccg tttctgctgc
    54301 cgatcggtat aggggaaacc tgagactaaa cgacagaccg caaacacgtc tggttcgttg
    54361 taccccgtct cgccttgtaa tatacgccgc cactgcggca tttttttcgc cagccaggct
    54421 ttcgctttgg gtaataactg gcaggttgag cgtggagctc cccagcggct aaggctgttg
    54481 tcgggataag caaacgcgag tatctggtcg taacgctccc cctgccccgc ctgggccgtg
    54541 gcttgccgcc aggaaagtgt cccttcggta gcgcgactgc catgataatg aaccggatcg
    54601 cctgcataga tgagctcctg cgtccaggcg gtcatcgttc gcgcccccgt ggtcgctggc
    54661 gaagcagaaa cacgctgcgt ggcgctgctg tcggggatgg tcaggcaatc accttcccga
    54721 ttggcgtttt gctgtaaaaa agtacgtatc gccacagtca tggctttcgc tgcctcaggt
    54781 ggcgtacttt tcgcctcgcg atccagaact ctggtaacgt actcttcacg atcgagatgc
    54841 gactgcaatt tcagtttgcc cttttcgctc agtaaggtcg tctcaccgtg gctgacaaac
    54901 gtaatgtggt taccattagt aaacgtcaca cgatagcgcc cattaagtac gctgggtttt
    54961 actgccgtcg tactcttttc cgctgtgatt ttcttcagcg gatagcgggc aaacagctcc
    55021 acctctacac attgcccact ggcaacctgc gtcggcactg gcaaaacccg attaagcacc
    55081 gtcgcataac gggttagtac cgttttgctg gtgccactgc cagtaaccca cagcggtgta
    55141 ccatcggtta accagccagc aaaaccaccc tgacggattt cctgtttatc atcggcaaac
    55201 cagctccagg ttttcacccg tacccgacta ccgagcatgg atgcgacacc gattttcgcc
    55261 tcgtccagca ccacatccag taagacttcc tgagctttgt tttgcgcagg taacgtcgct
    55321 aatgaatcaa gcagcgattt aaccgttacc gaggtttctg gcttcagcgt tgtaagagac
    55381 gctaaccacg caggtgcctg acgctgttgc cagtattgcc cccacacatc tgcgccaata
    55441 tgcaaccgct gcggcgcaaa atagagccca caggaacgta ccagcgcggt atcgcgcgtt
    55501 atgctctccc cagcctgaca acaataaacc tcttccgggg agtttccgcg acactggtag
    55561 ggctgctccg gttgatgggt atcctccagc caggcgtaaa catacagttt ccacaaactc
    55621 cctaacggcg tggtgagcga gtcaggtaat gctgagactt tggtcacgcc tgatgacgac
    55681 agctgataca gttgatcgtg ctgcgcaccg cgcagcacca gttgtaacgg cgtctcctcc
    55741 gcaagcgttg gcagagtaac caacgccaat agccagacga ttctgcgcca gttcacttat
    55801 ttcacctgca tcccggtcca ttcactccct gccgcgacac tttgctgcgc aggtgcatag
    55861 gaacgcacat aacgagcagg aggcagaacg aattgccctt tttgcgagaa gcgcagcaaa
    55921 tggcggaaag tgaccgttcc cgtcagttct ttcaccggca ccatataggc cagttcgccc
    55981 atttcattac gcgctttttc cagcaattgc ccctgctgtt tcgcggcgtt gggtttattg
    56041 actgaaatgc cccatgttgt gcgctcaacg tcggctcccg gcgggagcgg tacttccacc
    56101 tgaccatagc gcagaactgc atcctgctcg ctggtaagcg taatttcatc gagatacagc
    56161 gcatcgctgt caatctcatt gctggtcacc ggctgcagag taaagctcat ctcttcttca
    56221 ccggggataa gccgatacaa ctggcgttca acggtcaccg gaatgttact ttgttgagcc
    56281 gtttttgccg gttcacgcca gcggacctgc acatttgcgg cgataattcg tcaccaaaag
    56341 agagaatgtc cggcacaccc taaccaaccc aacgccagtc ttcgccccct ccagttaact
    56401 tatgtttagc ccatgcgccc gcaggcgcag gcaaaacaac tggaggcatt gtcgccatat
    56461 atttcgccag ccagttcatg gccagcgcgc gctcaatagt ggattgctca gcggtcaaac
    56521 cattaaaata gcggccgtct gggtagcgtc accaccgcta ttgagcagca caaccgtgtg
    56581 cgccagcggc tgattgctgt ttgccgcttt atcccacgca tattgctgaa cctgtggcat
    56641 caccgtggac ttcaactgcg cttttttcag caacgtcatg gttaagacgt ttgccaccgc
    56701 atctgccagt ggagattccg gtgtatcgag gatcaggctg tcattgatat cgcggatgtc
    56761 ttcctcagag aaatcttcat ctttagttcc gcgccgggcg atagcttcat cccccccttt
    56821 caacaacgtt ttgcacggca gattcatctc ctgtgcccac gccagcacca gcgcccgatg
    56881 caataacggc atgttatccg cctgctcggc ataactgtcg agcatatgct gccagtattc
    56941 cggttgttgc gttacgccga gcgcctggct ggcctgccag tcggcgtacc atgcccatgc
    57001 cgtaaggaag gcgtcaccat tgccatcttc accccaccag gtaaagcgcg ctccgggccc
    57061 cgccagttgc atcagccgca gacggttatc ctgaatcatc tgacgaatgt cgttagcggc
    57121 ggcactttga tgatcggcaa gcgaacgcca ggcgagactg agcgggatca gacggctacc
    57181 ggtgttgatc accccacccc acggttcatc gactaacgca tcaagattgt tgcggaaaat
    57241 ctcctgcggc gtttcactac tttgtagccg gatattactc gcctgctcgg gcaacatcag
    57301 cgcgttctcg ccaccgccga gcatgacatt tttctgctgt tcaacgggcc agctgttgtc
    57361 cacaaaagac agttttgtgc taatgctgtc ctgcacttgc ccattttgtt gcagttctgc
    57421 acttaacaag ccagattgct gaatattctg cgtcagcgaa atataattcg cccctttgtg
    57481 cagcgtcagc gtctggcgca tctcagcgcc tgcaaattta gtcaccagcg ccaccggttc
    57541 gttatcctgc tgactgaaga taaacagtcc tgccgccggt ttgtcgccca cgcgatacac
    57601 cgttggcata ctccacttca tgtagagatt tttttccgaa cgcagataag cacgcccctg
    57661 cccgaccagc ccgtcgccgt tcatcccacg cgcggtgata cgccagcggg ttaacgaatc
    57721 aggcatcagg aacgtgaaat acgctttgcc ttgtttatcg gttgtgagtg acggcatcca
    57781 tgccgcggta tccacctctt cacgccgtgg acgttcaagc atttttactc gccgctcgct
    57841 gcggttagtc gcgccaggcg caaccggctc gctggagagc gcctggtcat agctgataaa
    57901 cgacagactg gagctggtac gcacattgtt acgccccagc ggatagaaaa atttgccgat
    57961 attcggcgcg atttctggtt gcagcgcgta gatcatttca tcgaccacgc ctaccgttag
    58021 ctgcgcagaa acaggtttac ctttcagcga cgaggttaa
//
LOCUS       DAOFMX010000017        57829 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.017, whole genome shotgun sequence.
ACCESSION   DAOFMX010000017 DAOFMX010000000
VERSION     DAOFMX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.017"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..84
                     /locus_tag="QBA20_001171"
     CDS             <1..84
                     /locus_tag="QBA20_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDQ6291793.1"
                     /translation="RKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            804..1232
                     /gene="uspC"
                     /locus_tag="QBA20_001172"
     CDS             804..1232
                     /gene="uspC"
                     /locus_tag="QBA20_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDQ6291794.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1239..2653)
                     /gene="otsA"
                     /locus_tag="QBA20_001173"
                     /pseudo
     CDS             complement(1239..2653)
                     /gene="otsA"
                     /locus_tag="QBA20_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2628..3428)
                     /gene="otsB"
                     /locus_tag="QBA20_001174"
     CDS             complement(2628..3428)
                     /gene="otsB"
                     /locus_tag="QBA20_001174"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDQ6291795.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3595..4581)
                     /gene="araH"
                     /locus_tag="QBA20_001175"
     CDS             complement(3595..4581)
                     /gene="araH"
                     /locus_tag="QBA20_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDQ6291796.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4596..6110)
                     /gene="araG"
                     /locus_tag="QBA20_001176"
     CDS             complement(4596..6110)
                     /gene="araG"
                     /locus_tag="QBA20_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDQ6291797.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6180..7169)
                     /gene="araF"
                     /locus_tag="QBA20_001177"
                     /pseudo
     CDS             complement(6180..7169)
                     /gene="araF"
                     /locus_tag="QBA20_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7966..8469
                     /locus_tag="QBA20_001178"
     CDS             7966..8469
                     /locus_tag="QBA20_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDQ6291798.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8549..8800)
                     /locus_tag="QBA20_001179"
     CDS             complement(8549..8800)
                     /locus_tag="QBA20_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDQ6291799.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8915..9001)
                     /gene="azuC"
                     /locus_tag="QBA20_001180"
     CDS             complement(8915..9001)
                     /gene="azuC"
                     /locus_tag="QBA20_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDQ6291800.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9264..9587
                     /locus_tag="QBA20_001181"
     CDS             9264..9587
                     /locus_tag="QBA20_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDQ6291801.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9758..10255
                     /gene="ftnA"
                     /locus_tag="QBA20_001182"
     CDS             9758..10255
                     /gene="ftnA"
                     /locus_tag="QBA20_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDQ6291802.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10292..10531)
                     /locus_tag="QBA20_001183"
     CDS             complement(10292..10531)
                     /locus_tag="QBA20_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDQ6291803.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10721..11932
                     /gene="tyrP"
                     /locus_tag="QBA20_001184"
     CDS             10721..11932
                     /gene="tyrP"
                     /locus_tag="QBA20_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDQ6291804.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11994..12659)
                     /gene="yecA"
                     /locus_tag="QBA20_001185"
     CDS             complement(11994..12659)
                     /gene="yecA"
                     /locus_tag="QBA20_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDQ6291805.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12855..12941)
                     /locus_tag="QBA20_001186"
     tRNA            complement(12855..12941)
                     /locus_tag="QBA20_001186"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12905..12907),aa:Leu,seq:taa)
     gene            complement(12954..13027)
                     /locus_tag="QBA20_001187"
     tRNA            complement(12954..13027)
                     /locus_tag="QBA20_001187"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12993..12995),aa:Cys,seq:gca)
     gene            complement(13082..13157)
                     /locus_tag="QBA20_001188"
     tRNA            complement(13082..13157)
                     /locus_tag="QBA20_001188"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13122..13124),aa:Gly,seq:gcc)
     gene            complement(13309..13857)
                     /gene="pgsA"
                     /locus_tag="QBA20_001189"
     CDS             complement(13309..13857)
                     /gene="pgsA"
                     /locus_tag="QBA20_001189"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDQ6291806.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13914..15746)
                     /gene="uvrC"
                     /locus_tag="QBA20_001190"
     CDS             complement(13914..15746)
                     /gene="uvrC"
                     /locus_tag="QBA20_001190"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDQ6291807.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15743..16399)
                     /gene="uvrY"
                     /locus_tag="QBA20_001191"
     CDS             complement(15743..16399)
                     /gene="uvrY"
                     /locus_tag="QBA20_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDQ6291808.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16695..16871
                     /locus_tag="QBA20_001192"
     CDS             16695..16871
                     /locus_tag="QBA20_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291809.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16858..17082
                     /locus_tag="QBA20_001193"
     CDS             16858..17082
                     /locus_tag="QBA20_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDQ6291810.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17115..17735)
                     /gene="sdiA"
                     /locus_tag="QBA20_001194"
                     /pseudo
     CDS             complement(17115..17735)
                     /gene="sdiA"
                     /locus_tag="QBA20_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17965..18717)
                     /gene="yecC"
                     /locus_tag="QBA20_001195"
     CDS             complement(17965..18717)
                     /gene="yecC"
                     /locus_tag="QBA20_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDQ6291811.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18714..19382)
                     /gene="tcyL"
                     /locus_tag="QBA20_001196"
     CDS             complement(18714..19382)
                     /gene="tcyL"
                     /locus_tag="QBA20_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDQ6291812.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19397..20383)
                     /gene="dcyD"
                     /locus_tag="QBA20_001197"
     CDS             complement(19397..20383)
                     /gene="dcyD"
                     /locus_tag="QBA20_001197"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDQ6291813.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20488..21288)
                     /gene="tcyJ"
                     /locus_tag="QBA20_001198"
     CDS             complement(20488..21288)
                     /gene="tcyJ"
                     /locus_tag="QBA20_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6291814.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21376..21927)
                     /gene="fliZ"
                     /locus_tag="QBA20_001199"
     CDS             complement(21376..21927)
                     /gene="fliZ"
                     /locus_tag="QBA20_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDQ6291815.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21973..22692)
                     /gene="fliA"
                     /locus_tag="QBA20_001200"
     CDS             complement(21973..22692)
                     /gene="fliA"
                     /locus_tag="QBA20_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDQ6291816.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22857..24509)
                     /gene="fliC"
                     /locus_tag="QBA20_001201"
     CDS             complement(22857..24509)
                     /gene="fliC"
                     /locus_tag="QBA20_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HDQ6291817.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24775..26181
                     /gene="fliD"
                     /locus_tag="QBA20_001202"
     CDS             24775..26181
                     /gene="fliD"
                     /locus_tag="QBA20_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDQ6291818.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26206..26616
                     /gene="fliS"
                     /locus_tag="QBA20_001203"
     CDS             26206..26616
                     /gene="fliS"
                     /locus_tag="QBA20_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDQ6291819.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            26616..26981
                     /gene="fliT"
                     /locus_tag="QBA20_001204"
     CDS             26616..26981
                     /gene="fliT"
                     /locus_tag="QBA20_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDQ6291820.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            27059..28546
                     /gene="amyA"
                     /locus_tag="QBA20_001205"
     CDS             27059..28546
                     /gene="amyA"
                     /locus_tag="QBA20_001205"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDQ6291821.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28580..28993)
                     /locus_tag="QBA20_001206"
     CDS             complement(28580..28993)
                     /locus_tag="QBA20_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ6291822.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29180..30385
                     /gene="yedE"
                     /locus_tag="QBA20_001207"
     CDS             29180..30385
                     /gene="yedE"
                     /locus_tag="QBA20_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDQ6291823.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            30382..30615
                     /gene="yedF"
                     /locus_tag="QBA20_001208"
     CDS             30382..30615
                     /gene="yedF"
                     /locus_tag="QBA20_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDQ6291824.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30722..31392
                     /locus_tag="QBA20_001209"
                     /pseudo
     CDS             30722..31392
                     /locus_tag="QBA20_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(31432..32640)
                     /locus_tag="QBA20_001210"
                     /pseudo
     CDS             complement(31432..32640)
                     /locus_tag="QBA20_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            32708..33323
                     /gene="tnpA"
                     /locus_tag="QBA20_001211"
                     /pseudo
     CDS             32708..33323
                     /gene="tnpA"
                     /locus_tag="QBA20_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            33651..34733
                     /locus_tag="QBA20_001212"
     CDS             33651..34733
                     /locus_tag="QBA20_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HDQ6291825.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(35222..36020)
                     /locus_tag="QBA20_001213"
                     /pseudo
     CDS             complement(35222..36020)
                     /locus_tag="QBA20_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(36030..36581)
                     /gene="ybcL"
                     /locus_tag="QBA20_001214"
     CDS             complement(36030..36581)
                     /gene="ybcL"
                     /locus_tag="QBA20_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HDQ6291826.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(36750..37082)
                     /gene="emrE"
                     /locus_tag="QBA20_001215"
     CDS             complement(36750..37082)
                     /gene="emrE"
                     /locus_tag="QBA20_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HDQ6291827.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(37427..37741)
                     /gene="fliE"
                     /locus_tag="QBA20_001216"
     CDS             complement(37427..37741)
                     /gene="fliE"
                     /locus_tag="QBA20_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDQ6291828.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            37955..39620
                     /gene="fliF"
                     /locus_tag="QBA20_001217"
                     /pseudo
     CDS             37955..39620
                     /gene="fliF"
                     /locus_tag="QBA20_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            39613..40608
                     /gene="fliG"
                     /locus_tag="QBA20_001218"
                     /pseudo
     CDS             39613..40608
                     /gene="fliG"
                     /locus_tag="QBA20_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
     gene            40601..41287
                     /gene="fliH"
                     /locus_tag="QBA20_001219"
     CDS             40601..41287
                     /gene="fliH"
                     /locus_tag="QBA20_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDQ6291829.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            41287..42660
                     /gene="fliI"
                     /locus_tag="QBA20_001220"
     CDS             41287..42660
                     /gene="fliI"
                     /locus_tag="QBA20_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDQ6291830.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            42682..43023
                     /gene="fliJ"
                     /locus_tag="QBA20_001221"
                     /pseudo
     CDS             42682..43023
                     /gene="fliJ"
                     /locus_tag="QBA20_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            43020..44147
                     /gene="fliK"
                     /locus_tag="QBA20_001222"
     CDS             43020..44147
                     /gene="fliK"
                     /locus_tag="QBA20_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDQ6291831.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSVPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            44252..44716
                     /gene="fliL"
                     /locus_tag="QBA20_001223"
     CDS             44252..44716
                     /gene="fliL"
                     /locus_tag="QBA20_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDQ6291832.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            44721..45725
                     /gene="fliM"
                     /locus_tag="QBA20_001224"
     CDS             44721..45725
                     /gene="fliM"
                     /locus_tag="QBA20_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDQ6291833.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            45722..46135
                     /gene="fliN"
                     /locus_tag="QBA20_001225"
     CDS             45722..46135
                     /gene="fliN"
                     /locus_tag="QBA20_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDQ6291834.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            46138..46503
                     /gene="fliO"
                     /locus_tag="QBA20_001226"
     CDS             46138..46503
                     /gene="fliO"
                     /locus_tag="QBA20_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDQ6291835.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            46503..47240
                     /gene="fliP"
                     /locus_tag="QBA20_001227"
     CDS             46503..47240
                     /gene="fliP"
                     /locus_tag="QBA20_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDQ6291836.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            47250..47519
                     /gene="fliQ"
                     /locus_tag="QBA20_001228"
     CDS             47250..47519
                     /gene="fliQ"
                     /locus_tag="QBA20_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDQ6291837.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            47528..48313
                     /gene="fliR"
                     /locus_tag="QBA20_001229"
     CDS             47528..48313
                     /gene="fliR"
                     /locus_tag="QBA20_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDQ6291838.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            48598..49221
                     /gene="rcsA"
                     /locus_tag="QBA20_001230"
     CDS             48598..49221
                     /gene="rcsA"
                     /locus_tag="QBA20_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDQ6291839.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(49265..49453)
                     /gene="dsrB"
                     /locus_tag="QBA20_001231"
     CDS             complement(49265..49453)
                     /gene="dsrB"
                     /locus_tag="QBA20_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDQ6291840.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            49616..49843
                     /gene="yodD"
                     /locus_tag="QBA20_001232"
     CDS             49616..49843
                     /gene="yodD"
                     /locus_tag="QBA20_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDQ6291841.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            50141..50956
                     /locus_tag="QBA20_001233"
                     /pseudo
     CDS             50141..50956
                     /locus_tag="QBA20_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(50953..52647)
                     /gene="yedQ"
                     /locus_tag="QBA20_001234"
                     /pseudo
     CDS             complement(50953..52647)
                     /gene="yedQ"
                     /locus_tag="QBA20_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(52818..53000)
                     /locus_tag="QBA20_001235"
     CDS             complement(52818..53000)
                     /locus_tag="QBA20_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDQ6291842.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(53079..53996)
                     /locus_tag="QBA20_001236"
     CDS             complement(53079..53996)
                     /locus_tag="QBA20_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDQ6291843.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            54169..55089
                     /gene="yedA"
                     /locus_tag="QBA20_001237"
     CDS             54169..55089
                     /gene="yedA"
                     /locus_tag="QBA20_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDQ6291844.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(55078..55548)
                     /gene="vsr"
                     /locus_tag="QBA20_001238"
     CDS             complement(55078..55548)
                     /gene="vsr"
                     /locus_tag="QBA20_001238"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDQ6291845.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(55529..56947)
                     /gene="dcm"
                     /locus_tag="QBA20_001239"
     CDS             complement(55529..56947)
                     /gene="dcm"
                     /locus_tag="QBA20_001239"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDQ6291846.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEYHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(57014..57554)
                     /locus_tag="QBA20_001240"
                     /pseudo
     CDS             complement(57014..57554)
                     /locus_tag="QBA20_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(57594..>57829)
                     /locus_tag="QBA20_001241"
     CDS             complement(57594..>57829)
                     /locus_tag="QBA20_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291847.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT        14549 a        14791 c        13924 g        14565 t
ORIGIN      
        1 cggaagtcac tgtcgttctc aaaatcggtg gagctgcatg acaaggtcat cgggcattat
       61 ctgaacataa aacactatca gtaagttgga gtcattacca aaataattag cattagaata
      121 gttgcgataa gctgcaataa gcagagccac ctttttggtt taataagtcc ttacaaatag
      181 aagggggtct ttacacttat ctaagatttt tcctaaatcg acgcaactgt actcgtcact
      241 acacgcacat acaacggagg ggggctgcga ttttcaataa tgtgtgatgc agatcacaca
      301 aaacactcaa ttacttaaca taaatgtata agtcatactt ttgttttggg tgtatttcaa
      361 tctgttaaaa agtttttcgc tacgctagca agcaaaaatg aaacaggaat aatcgaaatg
      421 ggatgttgcg cacggttaaa ataactcacc gtaaataatc atctgctata aataatcact
      481 ttcatgcaat accagataag ctatttttaa acagacactt accgcacaac aaactaatta
      541 aatacaaaga aaggcaacaa tcaacttaat gttaataaaa tgtttttcat atattaacca
      601 cttgtttgat gcggggaatc agtacattat tttcttataa ttacagttga aatcatatga
      661 tcaccgagag tgattgatta attgtcttta ctaataatca gactaatatt tacctgtttg
      721 actgagttgg gattgcgtcg tttctccatt aggagtaaag ctttaatgtc acctgaagtt
      781 cacagcagaa taaggaacag gttattagct atagcaatat tcttgtcgct gttgcggtta
      841 caccggaaag tcagcaactg ctggcaaaag cggtatctat cgccaggcca gtaaagggac
      901 acatcagttt aattactctc gcttccaacc cggaaatgta caatcaatta gccgcgccga
      961 tgcttgaaga tttacgcagc gtgatgcaag aagaaacgca aagctttctt gataagttaa
     1021 ttcaggatgc ggggtatccc gttgacaaga cgtttattgc ctacggtgag ttaagcgaac
     1081 atattctgga agtatgtcac aaacatcatt tcaatttggt gatttgcggt aatcacaatc
     1141 acagtttctt ttcgcgagca tcctgctcgg cgaaaagagt gattgcctca agtgaggtcg
     1201 atgtgctgtt agttccactt acgggagatt aaccgctcct acgcaagctt tggaaaggta
     1261 gcaactttat cgcgctgctg gctttccgcg cttcgcggaa ctatctgctt taggtcgcta
     1321 atgaagcact cctgccagtg gttaatatcg tttttcacga taacgtccag catttctgca
     1381 tgacgggaaa tacgttccgc cagcgacata gtcaatgcac gatccagcgc agctgcaact
     1441 tcgtcacgat cgtaggggtt aacaattaac gccgacgtta actcgtttgc cgctcccgca
     1501 aattgcgaaa gaacaagaac gcccgggttg gctgggtcct gagcagcaac atactctttt
     1561 gctaccaggt tcatcccgtc acgcagtggc gtcactaagc ccacgtcaga gtagcggaat
     1621 attttcatca gtaatttacg gtcaaaatgc tgattcaaat aataaagcgg cgtccagcct
     1681 aattgcccgt atttaccatt aattcgtcca gcttcatttt cgagctgatg acgaatatcc
     1741 tggtaggctt gcacatcacc acgcgacgtt ggtgcaatct gggtataacg aattttacca
     1801 tgatgctgcg gatatttttc cagcaatgcc tcataggcga gaaaacgctc cggcaaacct
     1861 ttggaataat ccagccgttc gacagaaaag atattttgta cgtttttcaa ttccgcttta
     1921 agttgcgcca gttttggcgg cagtggcccg gcagcctgtt tggctatttc tttcggttca
     1981 atgccgatcg ggtagacttc tgttcgaaat gcttttcccc aggctgtatg gctttttgcg
     2041 ctacgtgtcg tgacgcgggt caggttagaa aggcaatcca ggaacgccag acgatcgttt
     2101 tctgtctgta aacccagcaa atcataatca caaagctgtt caagcaaggt gtcatatgtc
     2161 ggcagcgcgt tgaaaatttc cggtgtcggg aaaggaatat gcaagaaaaa accaatgcga
     2221 ttattcaccc cacgtttgcg taattcatgc gcaaagggca acaggtgata atcgtggatc
     2281 cagataatgt catcgtcttg caacagcggc agtaatttat ctgccagcaa cgcatttacg
     2341 cgtagatagc cgtcccaggc aggacgctga aattgcacca gatcgagccg ataatgaaaa
     2401 gcgggccaga gaacggcatt ggagaattgg ttgtagtatt cgtcaaggtc ctgttcgctg
     2461 aggttaaaag aggcccacgt aatgttacct tttttagcgg ctgatcctca ttccctgttt
     2521 caccactcca gccaaaccac agtccgcctg cggctttcag tgcccccagt atgccaacgg
     2581 caaggccacc ggcactggcg gcgtgctcgt ctggtggtgc aatccggtta gatactacga
     2641 ctaaacgact catagtcatc actcctgtta ttttctcttt tttgttgtaa tgcggcggtt
     2701 atcatttcaa gccagctcca gacatccggc acacccgcca gtcgccatga tgcctgagtt
     2761 gcacctgtgc caatttttac tgacattccg cccagtcggt taacgactgc gaagccagat
     2821 tcatcggtta aatcatcgcc cagaaatacg ggcgttcgcc cgataaaagg agcttcctgc
     2881 ataaaggctg caatcgcttc acctttactg gtacctctcg gtttgatctc gacaacacac
     2941 tttccctgct gtaacgccat ttgtggccag atctgagtaa tacgttgcgc taatgtcatt
     3001 aatgcgtctt catgctgcgg agcctgacga taatgcagcg caaaagccat ccctttcgcc
     3061 tccagctccg cgccgggata ctgagcgatg accgtatgca gttgcacgct aatatcacgc
     3121 gcaatcgcat ccggcagatg aacgatatgt gttttaccat tgatgtcacg gcgctccgcc
     3181 ccatgcacgc ccgctaacgg gaagcgataa ggttttgcca gtgcgtcaag ctccaccatt
     3241 gagcgccctg atatcaatgc caatgcacca tcacttgcgg ttgccagtag ctgtagtcct
     3301 tgcagaatat tgtcaggcac gacgacctga tcgggatgcg gtttgatttc cgccagcgtt
     3361 ccatcaagat caaaaaacca ggcatatttc gcggatagtt caggggtttc ggttaacggt
     3421 tctgtcaccc ggctctcctc cttctctttt cttattgtgt tgcgcagacc gcgctttaca
     3481 gacatgtaag tatagacagt gtgacggagg tcgccatttg caacgaagaa cagccaaccg
     3541 ttgtggcggc tggctgtggt gggaaaaaac gttaaattgt tgtggaaaaa agcatcagac
     3601 agtgcgcttc gctttttgct tgtaacggtc gaagatcacc gctgccagca ggattaagcc
     3661 gcgaaccacg tactgcgcga aaggagaaat attaagcagg ttcatggcgt tttccacggt
     3721 gcctaaaatt aagatacccg ccaccacata tgagattttt ccgatgccac ctttcagaga
     3781 aacgccacct aaaacgcagg cggagataac aataagctca taaccaatcg acgtcattgg
     3841 ctgcccacta gtcatacgtg aagccagaat aattccggct atcgcagata ccaggccaga
     3901 gagaacaaag ataataattt tggtgcgaac aaccggtaca cccgccagac gcgcagcctc
     3961 ttcgttcccg ccaatcgcca gggtattacg accaaaggtg gttttattca gcagcaaacc
     4021 aaagataatc agacacgcga cggtgagcca gattggcgca ggcagaccga accagttagc
     4081 gtaaccaagg gcaaagaagc tttcatcttc gataccgacc gctttaccgt ctgaaatgat
     4141 gtacgccaga cctcgaacaa tctgcatcgt tgccagtgtc gtgatcagag catttatttt
     4201 cagtttggcg ataacaaagc cattgaccag gccacagaga acgcccagca acaaacccgc
     4261 tgccacgcca atccacaggc tttcagtcag gttgataacc accgccgtgg tgacacccgc
     4321 acaggcaatt acggaggcga cagaaaggtc aaagtcaccg gaagcaaggc agaacaacat
     4381 gccacacgcc accatccccg acatggaaat tgccaggccc aaccctttca tattaatgaa
     4441 ggtggcaaaa tttgggacaa aaatggcaca ggcgataaag agcaccgcaa acaccaccag
     4501 catgccgtac tgatcccaga tacgcccgaa gctgaatgac gacttaggtg cgccagaccc
     4561 cgatgtagaa acagaagaca tcatactctc cttactcagg caactgcctg gctgacttta
     4621 ggcatcgcaa ggctcagtgc ctgacgctca tctgcctgct cgtgtaacaa ttcaccggcg
     4681 atttcgcctt cccgcatcac cacaatccgg tcggcaacgc cgaggacttc aggtaagtcg
     4741 ctggaggcaa acagcaccgc cacgccctgc gccgccagcg cataaattac gttatatatt
     4801 tcgtgcttag cgccaacatc aatgccgcgc gtaggttcat ccagcaaaat gaccttcatc
     4861 tcttccgata accagcggcc cagaatggct ttttgctgat ttccgcctga gagattcatg
     4921 atcagttgct ccgcgcccgg cgttttgatg ttgagcgaac gaatgtgctg atcggcattg
     4981 ttttcttccc aactgttgtt gattacacaa ccgccgagca catgtttgcg tctggcactg
     5041 atgttgatat tgtcgcgaac ggagtgcacg ggaataatgc cttccgcttt gcgagcttcc
     5101 gggcagagca tcatgcctgc ggcaatggcg tggctcggtt tacgaatatc gatcggctgt
     5161 tggtcgatat aaacctgacc ggcggtgatt tgcgtcccgc caaacaagcc tttcattaat
     5221 tcgctacgcc ccgcccctac cagaccaaac agcccaacaa tttcaccact gcgaaccgcc
     5281 agacttattg gcgtacgcac gcctagtgct ttcacagcat caagacgcag gcgctcctcg
     5341 ccataactac gaggttgcca gccgtagata tcgccaatgt cgcgcccgac catcgcctgc
     5401 accagcgcgt cgtggtcaac ctgctgcata tcggtaaagg ttttgacata acgtccatct
     5461 ttaaagacag tgatggcatc gctgagggca aatatttctt ccatacggtg agaaacgtat
     5521 aagatcaccc gcccctcttt tcgcagttca cgaataacgc ggaaaagatt gccgatttcg
     5581 cgggcagaaa gggagctggt tggctcatca aaggcgataa ttttagcgtt acgcgccagc
     5641 gctttggcga tttcaaccat ctgccactga ccaatggcga gatatttcag cggcgtgtcc
     5701 gggtcaatat ccataccaag atgtttaagt tgtaaacccg cctcataatt cagcaatgag
     5761 cgattcacaa tgccgccttt atgcggcagc tggccgagat agatgttttc cgcgacggtc
     5821 atttccggca cgagatgcag ttcctggtaa ataatcgcca cacccgcgtt aagtgctgcg
     5881 gtcgtgtcgg aaaaggacat ttcctgccca ttaatcacta cagaacccgt ggttggcgca
     5941 tagttgccgc tgaggatttt taagagagtt gattttcctg cgccattttc acccatcaac
     6001 gcatgaacct gaccggcata gcagtcaaaa ctaatatccg tcagcgcctt aacgccggga
     6061 aacgttttac cgatgccgcg aaatgagaga tacggggtag actgttgcat aacgtctccg
     6121 tgaatcactg gtcgttcaca ccccctctgc atcatgcaga ggggaatttt tccggcaaat
     6181 tacttaccgc ctaaaccttt tttctccagt tcttctttaa agttgtcacg cgtgatcagt
     6241 accacgtctg taacttcggt aaattttggc gtttcaacgt cttttgctac ccagttgtaa
     6301 agcatttcgc tggatttata gccatgtacg tccgggcttg gcagcaggga accgtagaag
     6361 ccggttgcct gtgctttaga cagttcgctt actgcatcca caccgttaat accaataccg
     6421 atgatatcgg ctgctttaaa gccctgacct tcggttgcac gtacgccgcc aagtacggtg
     6481 ctgtcgttca taccgacgat cagccaatgt ttaacttccg gatgttgaac cagcattgag
     6541 ttggcagcgt caaatgcccc cgggatgtcg ttagatttgg taggtacctg ataaatttgt
     6601 ttttccggga atcctgcagc tttcagtgcg tccatagaac ccgttgtacg gcggcgggcg
     6661 gtatccagtt cgttggcggt aatcgccatc actgcgcttt ctttgacatc ccagccacgt
     6721 ttctgcatct ctttatacag ttcctagccc tgacgttcgc caattttagt cgccgccatc
     6781 atcaccagag gaacggtatc cattggctta cctttggcgt taacaaactg gtcatccact
     6841 gcaatgactt tcatatcgta gccacgcgct ttcgcgacga tggcagagcc gagtttcggg
     6901 tccggagtac aaataacgaa accttttgcg ccactggctg ccaggctgtc gatcgcgttc
     6961 aatgtttttt cgccatccgg cacggcaatc ttaataacct caaaccctaa atccttcccg
     7021 gctttatcgg caaacttcca ttcggtctgg aaccacggct cttccggttg cttcaccaga
     7081 aaaccgagct tcaggttctc cgccatagcg gattgtgaca taacggctgc cagaccaatg
     7141 gctgccaggg ctttagtaaa tttgtgcatg gttctctcca gctttagtgt cgttttgtgt
     7201 agtgcaaaaa cgaatgacat tcgttaaatt aatcggaaaa caaagcatta ccttttatct
     7261 aaaagataag tgaccgtgtt gacatagttt tagcgagaaa ttaattctct ataggagagc
     7321 aatatcacat cgcagaatta cagtgagaac gtgcataaat ttagcgggaa aagacataag
     7381 gaaaagccaa tttgtcagac aaattgttga atgcacagca gattaatcca taagattagc
     7441 ctggaaatcc ttgttgtctt cggtacccat gcgggatgtc ttctttttaa ccagtcaata
     7501 ggccgcatta cctggcgttg agtttttgaa atggtgtaat aaccacaact caaagatgtg
     7561 gaaaatgcgc gtcattcatt tcgccattaa ttatcactgt gctcattaat taacagaaca
     7621 cgtataatga gagccatctc gcaaaaatga aaaaacgttt tataaaatca tcacttcatc
     7681 atgaattcaa attcatcgat taatatcaat aagatacaaa aagcactatc attaaaattc
     7741 attgcattta cattgatttc accaatgaaa tgtaaaaata tataaacttg atgatttaag
     7801 cattttctta taaccgttca gacgttattc ttatttcaga tcatcgtcag aattgactcc
     7861 acgatcacat ttcggaccgg cagaaaggaa ttattctgca aacagtaatt atggtgtttt
     7921 gatttatctt gcacctctcc acttctggat ataaggatat taggtatggc aaccgctgga
     7981 atgcttctca aactcaactc tcaaatgaac cgcgagtttt acgcatccaa tctctacctt
     8041 cacctgagta actggtgttc tgaacagagt ctgaacggca ccgccacttt ccttcgcgcc
     8101 caggcacaga gtaatgtgac ccaaatgatg cgcatgttta actttatgaa gagtgtcggc
     8161 gctaccccca tcgttaaagc cattgatgtt ccaggtgaaa aactgaactc tctggaagaa
     8221 ctgttccaaa aaacgatgga agaatacgag caacgttcaa gcactctggc gcagttagcc
     8281 gatgaagcga aagaactgaa tgatgattca accgtcaatt tcctgcgcga tctggaaaaa
     8341 gaacagcagc atgatggcct gttgctgcaa accattcttg atgaagtgcg cagtgcgaaa
     8401 cttgcgggta tgtgccctgt gcagaccgac caacatgttc tgaatgtcgt gtcacaccag
     8461 ctgcattgat catacatcgg cgctaataca ttgcgccgat gaagtttttg agaaaccgct
     8521 gcctcatctg tttgaagcag cggttttttt aatgggattc accctgtggg gtaaacttga
     8581 gttcaatcag cgcgatggct ttttggattg cccgcatggt gaccgggtct gcggcagcgg
     8641 gatgatgagt aaagtcgata ttcttcagct gactggacat tttttcacga acttcaacgg
     8701 gcgcgattac atcgagaaca tccagaattt gtttgataac caactggcaa gctaccacat
     8761 cagaaaccag ttcctgatcg gcattcagcg gctgggacat cgtaaactcc tgatagcatt
     8821 ttgaaagccg ttatagtagc ggcttcacat cttcagcgat agtcacatcc accgtcatca
     8881 ggacacaaaa aaacctgccg gagcaggttt tttgttatcg gaacatattg cctggcggta
     8941 cgtctttgaa cgtcttgcaa tagttattga acatactttt caggattttg cgcagtttca
     9001 tcgcggcact ccgaccattt gttatacagg tgttattgtc tttgcgctta taatatgaca
     9061 accatcacaa aaatcaatct ttatgtgata caaatcacat aaatacccct ttaatgttat
     9121 aaaaatgata atcaaaaaac agccccccta tttctgacac ctacagatgg caagaaatag
     9181 cgcctgccag gcgtcttttc cggccattgt cgcagcgctg tgacgcgtaa aatagtgctt
     9241 tctcttactc ttctggctgg accatgagac ttctgattct gactctttca ttaataacgc
     9301 tggcggggtg tacggttact cgccaggccc atgtgagcga agttgacgcg gcaacaggta
     9361 ttgtacggtt ggtttatgac caggcttttt tgcagcatgc ccataccgat cgttatgtca
     9421 gtcgcgggat cgccgatcgc gcatgccagc aggaaggcta tacccacgcg atcccctttg
     9481 gtcagccagt aggcaactgt agcctttttg cgggttcctt atgtttgaat acggaattca
     9541 ctttgtcata tcagtgccat cattccgcct tccctgtctt tctctaaata ttttaatcag
     9601 cgaggggatc ttcgctgatt aaagaaatag cggtaatgcg ttttattccc attcgtattt
     9661 ttaataattg aagtttatat tttacctttt gcaaataata aaataacaaa ttatagtgac
     9721 gccacagcaa caaatataac ctttgtggag cactatcatg ctgaaaccag aaatgattga
     9781 aaaacttaat gagcagatga acctggaact gtactcttca ctgctttatc agcaaatgag
     9841 cgcctggtgc agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc gtcacgccca
     9901 ggaagagatg acgcatatgc agcgtctgtt tgattacctg actgataccg gcaatttacc
     9961 gcgtattaat accgttgaat ctccgtttgc tgaatattcc tcacttgatg aattattcca
    10021 ggaaacctat aaacacgaac aattaatcac ccagaaaatt aacgaactgg ctcatgctgc
    10081 aatgaccaat caggactacc caacatttaa tttcctgcag tggtatgttt ctgagcagca
    10141 tgaagaagag aaactgttca aatcgattat tgataaatta agcctggcag gcaaaagcgg
    10201 cgaaggtctg tattttatcg acaaagaact ctctaccctc gacacacaaa actaatgctc
    10261 tccggcggca ggtttctgcc gccgtttcaa atcagtgacg gcaaatctta ctctgatcgg
    10321 tcgaaaaacc ttcttctgaa ggtataaatt tgccttttgc tgccagaaac gctaccagct
    10381 ctcccgctgt caccccttct gccgagcacg tgtgaaaacg tgcctgttca ccaaaacgcg
    10441 ctttaatcgc agcttccaga ctggcatgcg tatattgctc gcctgattca atcatcatat
    10501 ttaacacttc atgaccgtga atagagtcca tcgttcctcc tcaaaaaaag cctagcgtag
    10561 cgattaccgc ttatgaagac tttgcgccag cgcaggactg aatgctttta ttgtacattt
    10621 atatttacac aatatgtaac gtcggtttga cgaagcagcc gttatgcctt aacctgcgcc
    10681 gcagatatca ctcataaaga tcgtcaggac agaagaaagc gtgaaaaaca gaaccctggg
    10741 aagtgttttt atcgtggcgg gaaccacaat tggcgcaggc atgctggcaa tgccgctggc
    10801 tgcggccggt gttggtttta gcgttacgtt aatcttgttg attgggcttt gggcgttgat
    10861 gtgctacacg gcgctattac tgctggaggt gtaccagcat gttccggcag ataccggtct
    10921 gggcacgctg gcaaaacgct atctgggacg ctacggtcaa tggctgacgg gcttcagtat
    10981 gatgttctta atgtatgctc tgactgcagc atacatcagc ggtgccggtg aattgttggc
    11041 ctccagcatc agcgactgga caggtatttc aatgtcggca accgctggcg tgctgttgtt
    11101 cacttttgtt gccggtggcg tggtttgtgt tggaacatcg ctggtcgatt tatttaaccg
    11161 ttttctgttc agcgccaaaa ttatttttct ggtggtgatg ctggtattgc tgctaccgca
    11221 tattcacaaa gtgaatcttt taaccctgcc gttgcaacag gggctggcac tgtctgcaat
    11281 cccggtgatt tttacgtcgt ttggttttca cggtagcgtg ccgagtattg tcagctatat
    11341 ggatggcaac attcgtaagc tacgctgggt gtttataatc ggtagtgcca tccccctggt
    11401 ggcatatatt ttctggcagg tggcgacgct tggcagcatt gattcaacaa cctttatggg
    11461 attgctggct aatcatgctg gattaaacgg gctgttacag gcgttacgcg aaatggtggc
    11521 ctctccgcat gttgagctgg cggtgcattt atttgctgat ttagccctcg ccacgtcatt
    11581 tctcggcgtt gcgttaggct tatttgatta tctggctgat ttgtttcagc gttcaaatac
    11641 cgttggtgga cgattgcaaa ctggtgcaat tacctttctg ccgccgttgg cgtttgcact
    11701 gttttatcca cgaggatttg tgatggcgct gggttacgcc ggtgtggcgc tggcggtact
    11761 ggcattgatt atcccttcac tattgacctg gcaaagcaga aagcacaatc ctcaggcggg
    11821 ttaccgggtc aaaggtggtc gtccggcgct ggtggtggtg tttctctgtg gtattgctgt
    11881 gattggcgtg caatttttga ttgcggcagg gttgttacca gaagtggggt gatcagatag
    11941 cctcaaattc cttattgggt gccagaatta acgctgacac ccaatgtgac ctcttaatgc
    12001 aggcagcact gcttaaattt cttaccactg ccgcacggac aaggatcgtt acgccccggt
    12061 ttctcttctg ctttgatcgg ttgctgaaca gctttttcct gcggatgcgc catccagtat
    12121 gcatgtaaat caagcgccgc cagtcgaatg gcatctacgc tctcttcaaa cgcttctggc
    12181 gacattttct ctacccgctc gaagttttcc tcagtaccgt gcagcgcaat cgcctccagc
    12241 gctggtttta aagaatcggg caacgttgac cagtcagaaa gtgccacgcc ccgcatatag
    12301 ccaaagcacc actcctcaac aatcgtcagc tcgctgccat caacttctcg caagccgaat
    12361 aacggctcaa actgctccgg gaattcgttc agacgctctg cggtatcggc catatgttga
    12421 aaagccaggt tcataaagcg cgtcatctct ttctctgacg cccaacgcgg cacatagtca
    12481 gccccacccc acacggcaac cagccactgt tctggttcaa tctcttgcgg agaactcaac
    12541 accgccgtca ataaaccgtc cagctccgcc acatcaagga tggcgtggtc agtgttgtat
    12601 ttggtcagaa tatcgtccag ccattccaac tcactttcgt ttaacggtcc cgttttcata
    12661 cgcttttcct tgtggatctc aactcgccag cacctatctt acatgccggt ccgtatcaga
    12721 gatacttttt gagtggcttt gccggtgatt aaaaattaag gagggtgtaa cgacaagttg
    12781 caggcacaaa aaaaccaccc gaaggtggtt tcacgacact gcttattact ttgattttat
    12841 tcttatcttt cccatggtac ccggagcggg acttgaaccc gcacagcgcg aacgccgagg
    12901 gattttaaat cccttgtgtc taccgattcc accatccggg ctcgggaaga aagtggaggc
    12961 gcgttccgga gtcgaaccgg actagacgga tttgcaatcc gctacataac cgctttgtta
    13021 acgcgccaaa ttcttcaggc ctttcagcca gacatccgct tgacgccgat gtcttttaaa
    13081 ctggagcggg aaacgagact cgaactcgag accccgacct tggcaaggtc gtgctctacc
    13141 aactgagcta ttcccgcatt catcaagcaa tcagttaatc acttgatttt attatcgtct
    13201 ggcaatcagt gccgccgttc gatgcgttgc attctactta cctggcgcga tgagtcaacg
    13261 atatttttca ccacttttga tcgtttgctg aaaattacgc cgaaacgatc actgatcaag
    13321 caaatctgca cgcgcagcgc tcaaatattg caacattgac cacagagtca gtaccgcagc
    13381 cacaaagaaa agtgcaatac cgacgtactc aacccaaatg ttcggacgcc acagcagcca
    13441 ggccaacgcc accatctggg cagtggtttt cactttccca atccaggaga cggccacgct
    13501 actgcgttta cccaactccg ccatccattc gcgtagcgca gaaataataa tttcacgggc
    13561 gatcatcgtt gccgccggta atgtcaccca ccagctgtga taatgctcgg ttaccagcac
    13621 catggcgata gccacgagaa ctttatctgc cacagggtca aggaaagcac caaaccgggt
    13681 actctggttc cagcggcgtg ccagaaaacc atcgaaccag tcagtcaccg ccgcgacgca
    13741 gaaaatgagc gcggcggcaa acggcgacca ggtgacaggc agataaaaga ccaatacaaa
    13801 gaatgggata aggatgacac ggaacagtgt aagcaacgta gggatattaa attgcataat
    13861 gacgggtaac tatctgttgt cagtaagatt acccctatgt tgctacagag acatcaatgt
    13921 ttcaacgacc agaagatctt ttctgccaga ccttgcgaaa tacccggcac ttttgcaatt
    13981 tcctcgacgc tggcgttacg taaaccttgc aaaccgccca tatatttcaa caacatttgc
    14041 cgacgttttg gcccgacgcc ttcaatggtt tccagggaac tggtattttt gaccttcgcc
    14101 cgttttttac ggtgcccgcc aatcgcgtga tcatgtgatt catcgcgaat atgctggata
    14161 acatgcagcg cgggtgaatc tggcggcaaa ctaaatccct caccttccgg ctcaaagaac
    14221 agcgtttcca gtccagcctt acgatctgct cctttggcaa cgccaagtag cagcggatga
    14281 tttttatccc atgagacatc cagttcggcg aagacatttt tcgcctgcgc aagctggcct
    14341 ttgccgccgt cgataaggat cacatccggg atcttactgt cgtcaatggc tttaccataa
    14401 cgccgacgca gcacctgatt catcgccgca taatcatcgc ccggcgtgat gcctgtaatg
    14461 ttatagcgcc gatactccgc acgcagcggg ccgttagcat caaacaccac acaggaagcg
    14521 acggtttgtt cgcccatggt atggctgatg tcaaagcact ccatccgctt cacttccggc
    14581 aatttcaaca cgctggcaag cgcggtcagt cgctggtgaa cggtagattg ctgcgaaagt
    14641 ttgctggtta aggccgtcgc cgcattggtg cgcgcgagtt tcagataacg cgccctatcg
    14701 ccgcgaggtt tggtttgaac attaatcttg cgtcccgcca gttctgaaag ggaatcggcg
    14761 agcagcgttt tatcgctaag attaaaatcg agcaggatct cacccggtaa ggtgcgcatc
    14821 tggctgcctt gtaaatagaa ctgacctacg aaggtttcta ccacctcgct cagttccgta
    14881 ccgccaggca ctttcgggaa atagctgcgg ctgccgagca ctttgccctg acgaatgaac
    14941 aatacgtgga cacaagccat gcccgcatcg aacgccacac caataacgtc aaggtcgtcg
    15001 ccggtattgg aaacaaactg tttttcggtg acgcgtcgca ccgcctgaat ttggtcgcga
    15061 atacatgccg cttcttcaaa ctccagattc tggctggcag tttccatacg gctaatgagt
    15121 tgcgtaagca cctgatcatc tttgccagac aaaaacaggc gcacatactc gacctgctga
    15181 gcgtattctt cttcactcac cagtccttca acgcacggtc ccagacagcg ccctatctgg
    15241 tattgcagac acggacgcga gcgattgcga taaacactat tttcgcactg gcgaatgggg
    15301 aaaatctttt gcagtagcgc cagtgtttca cgtacggcat agccattcgg gaacgggccg
    15361 aaatattcac ctttggcatg ctttgcacca cgatgcatcg ccagacgcgg gtgggtatcg
    15421 ccactcagga agataaaagg ataagattta tcatcgcgta gcaaaacgtt gtaacgcggc
    15481 tgatagagtt tgatgtagtt gtgttccagc aacagcgctt cggtttctgt atgcgtaacc
    15541 gttacatcaa tttgctggat ctgggcgacc agcgcttcgg ttttgcgcga agcgaggttg
    15601 ctgcggaaat agctggaaag ccgttttttc aggtctttcg ctttgccgac atagataacc
    15661 gtaccaccag catcgtacat gcgataaacg cctggctggc tggttacggt ttttaaaaac
    15721 gcttttgcgt caaactgatc actcactgac ttgataatgt ctccgcatta cacagaccat
    15781 ggcgaattgc caggtgagtc agctcaacat cgccatgaat gtttagttta ctgaacatac
    15841 gatagcggta gctgttcacc gttttcggac tgagattgag ctgttctgag atctcattga
    15901 ccttctggcc cttagtgatc atcagcataa tctgcaattc acgttcagac aaactggcaa
    15961 atgggctttc tgttttttct ggttcgatct ggcttaacgc catttgttga gcgatatcag
    16021 aagcaatgta acgctgccct gaatagacag aacgaatcgc actcacgact tcctgcggag
    16081 ccgcgccttt gctgaggtag ccagcagcac ctgcctgcat gactttcgct ggtaaagggt
    16141 tttctgtatg gaccgtaagc atgatgattt taacatcagc tgtggaacgc gcgattttac
    16201 gcgtcgcctc aagaccgcca atgcccggca tactcatgtc cattagcacc acgtcaacgg
    16261 catttgtccg gcaccactta acggcgtctt caccgcacga tgcctctccg acgactttta
    16321 tacccttaat atcttccaga atgcgtcgta tccctgcgcg caccagttcg tggtcatcaa
    16381 caagtagaac gttgatcaaa ggaatatctc cagaaatagg gataacgcta ctgatagtta
    16441 gtcattcgta tattagcggt ttttattgca actttgaaac gttaaaaatg gtgggtattc
    16501 gatttttctc tcgtttttgt caattcgatt gtccacagtt atggtcacgc ccgtcaccaa
    16561 cggcttaccg gatcgtttca cagtaagccc tacgatgaaa tgtgacaaaa attgacttta
    16621 ttcagcaaaa atgaaaatca gcccgctgaa tatgtaacat taattaaccc gaacaatacg
    16681 ggcaaaaaac attcatgata aaaatattta ttggtcatta tattaacgta ttttatagca
    16741 ctgccgatat cacgctcaaa aaacaaccac tgctattttt agcaaagctt atggtatact
    16801 ccgccgcctt aacatttttc accacaaatt ttcattgcaa catgacgagg aaaataaatg
    16861 agtacgcctg atttttctac tgccgagaat aatcaagaac tggcaaatga agtctcctgc
    16921 ctgaaagcga tgctgacgct gatgctgcag gcgatgggac aagctgacgc gggccgcgtg
    16981 atgttaaaga tggaaaaaca gcttgcgctg atcgaagacg aaacccaggc tgcagtattt
    17041 tccaaaacgg ttaagcaaat taaacaagcc taccgtcagt aatataaaac cggctgagag
    17101 cgtgcctttc agccggttat tgacaaaatc atcgctatct ctgctgatgt cttcccttcc
    17161 gccgtccatt tcagaatttc tttttcgcgc ttgctgaaat tcatctctgg cgtcatcact
    17221 atttcatcat ttaaacgcat cagagccatc agactttcgc gcaccagtaa ctgcattttt
    17281 aattgcagtt catcactaag aatgggtatt tcgcgcgtgc tgcaacggga aaaggacaaa
    17341 aagcccagcg cccgatttgg cagcattaaa tactgagtga caccgcggcg taaaccatgt
    17401 gcgtgcgcgg cttcccataa tggctgtgct tcgctgaata agtcatcatt ccacattaaa
    17461 tggccctgac taaagttttc agggttcagc acaggatcaa ttgcgagaaa attttttgcc
    17521 tgataataac taacccacga ctcagggtaa ttggtgtaga aagccacttt aggtcgcgtg
    17581 aatggtaccg ggtggcgaac acataacgaa tagtaatcgt actccagctg ctgagcctga
    17641 agctctattt catggtaggc ctcttctgcg gcctccatcc tctgaaaacg caacagcatc
    17701 gtgcgacgcc agctgaaaaa atccgtatcc tgcatagtaa accgcaacgc ccctgagagt
    17761 gagtatcatt tataatgata attcaaacta tcacataaat aagatttata tataatttat
    17821 attattcagg caatgaatta cttttgcaag ccatcgcatt ctcttatgtt attaatgagt
    17881 tatgctgatt tgttaagcag ttttatcacg cttgaaatgg cgtcccgccc cgacaggtga
    17941 atcgtcgggg ctgatttttt cttattattg cagcagaagc ttctcgagga actggcgggt
    18001 gcgaggctgc tggggatcgg caaataacgc ttttgctgcc ccctgctcga ctatccgccc
    18061 ctggtccata aagatcgccc ggtccgcaac atcccgggca aagctcattt cgtgtgtcac
    18121 aatcaccatc gtgcgctttt cctgcgccag ctggcggatg gtgttcagga cttcgcccac
    18181 cagctctgga tccagcgccg acgttggctc gtcaaacaaa atcacctcag gacgcattgc
    18241 cagtgcacgc gcaatcgcaa cacgctgctg ttgaccgcca gacaaacgac gtggatagct
    18301 ggtttcttta ccggccagcc caactttcgc cagcagctcg cgagcgcgcg ccgtggcctc
    18361 ttctttcggt tcgcctttga cgatcaccgg cccttcaata atgttttcca gcaccgtacg
    18421 atgcggaaac aaattaaagt tctggaagac aaacccgacg tgctgacgca actggcgaat
    18481 cagcgatttt tgctgactta atgaacgtgc agtatcaata gtgatatcgc caacggtgat
    18541 cgttcccgct tccggttgtt ccagcagatt tatgctacgt agcaatgtgg ttttgccgga
    18601 accactcgga ccgataattg ccaccacttc gccaggcttt acctcgaggt cgataccgtg
    18661 cagcaccgtc tgaccgtgga attttttcac caggttctta acttcaatgg cactcatttt
    18721 ggttctctct cctggcgatt aagttgattc tcaaaatggt tctgcaacgt cgataacaca
    18781 gtcgccatga tccagtagat gagcgaagcc gccaggtaca tggtgaacac ctccagcgtg
    18841 cgcgaggtaa tcaactgcgc ctgacggaac agctccggca cctggattgt cgcggccagc
    18901 gacgtatctt ttaccagact gataaagctg ttcgacagcg gcggcaacgc cacccgcgcc
    18961 gcctgtggca aaatcgcgcg acgcatggtc tgccacggcg tcataccaat actggcagcg
    19021 gcttcccact gacctttatc aatagaagaa atggcggcac gcagcgtttc ggcggcatag
    19081 gcggcagtgt tcagcgacag accaatcatt gctgacggaa tgggatctaa ttcgatacca
    19141 aactgcggca ggccgtaata gatcataaac agttgtgcga tgagcggcgt accacgaaaa
    19201 atagagatat aaaaacgcgc cagccagcgc acgggccaga ttggcgacag gcgcatcagc
    19261 gcgagaataa acccgagtag taagccaaaa aacatgccgc cgatactgag ttgcagggta
    19321 tacccagccc ctttcaacag gaacggcaag gaatcaataa ccagttgtat actttcttgc
    19381 atgagcgttc ccggagctaa acgtggggat gataggcgaa cagcgcaggc gcgccgccgg
    19441 tatgaataaa cagaatcggc ccttcatctt tgaagcgttt ctgactgata ccgtcaatca
    19501 gccccgccat cgcttttccg gtatacacag gatcaagcag aatgccttcc agacgcgcca
    19561 gcaatttcac tgcttccatg ccttcgtcgt tcggcacgcc gtagccaggt gcaaaatagt
    19621 catcccagag taaaatttcc gctgatgcgg tcagctccag ttctttcgca atcgcctgtt
    19681 gtaggttaac cactttcggc aattgatcgg caacggaacg cgacacggtc acgccaatca
    19741 gttcgctttc aggcatcagg tgttccagcc caacagccag tccggcgtga gttccggcac
    19801 tgcccgatgc gactaccacc gacgaaatat taaccgcccc ttcacactgt tgcgcgattt
    19861 ccagcgcact ctccacataa cctagcgcgc caagagcatt agaaccgcca accggaatga
    19921 catacgggcg aaagccttgt gcttcgactc gcgtcgccag ttcttccagt tgggcattgg
    19981 gatcggtcag tgcgtcgcac atttcaatct gggtattgaa cagatccagc aacaaacgat
    20041 tgccgttggt taaatagttt tctgcggttg tgccaatagg attttccagc agcgccacgc
    20101 agtgcagacc gagtttcgcc gcgactgcgg cagtctggcg cacatggtta gactggatcg
    20161 cgccggcagt aatcagcgta tcggcacctt cgcgcagggc atctgccgcg agaaattcca
    20221 gcttacgtaa tttattgccg cccattgcca tgggggtgac ctcatcccgt ttgatgaaaa
    20281 tttcccgtcc aagataatca gaaaagcgcg gcagatattc gagcggcgtt ggcgcgccga
    20341 taaactccaa ccgtggaaaa cgggttaaat tatgcagtgg cataacagcc tccgatgtgt
    20401 gttgttgtga ttttcttatt atgcacgctg aaaacgcgta aataaaaaag gcgctagtga
    20461 aagcgccctt ttttgtcatt atgctgatta tttggtcaca tcagcaccaa accatttttc
    20521 ggaaagagct tgcagagtgc cgtctttttg catttccgca attgcatcat tcaccgcttt
    20581 cagcaggtct tcatttcctt tacgcagcgc cacgccagac tcctgacggg agaatgcttc
    20641 accggttact gccagcgtat cgttggtttt cttcaccaga tccagcgccg ccagacgatc
    20701 aacgaggatc gcatcgatac gccctacgcg cagatcctga tatttggtcg ggtcatcatc
    20761 ataggtacgc acatcgacac cctgaacatt ctgccgcagc cactcttcat agttggtgcc
    20821 cagaccgaca cccacttttt tgcctttcag atcagcagcc gttttaatgg tgccttcgtt
    20881 accttttttc accagcgcct gaataccgga aatggtgtaa ggggtagaga aatcgtattt
    20941 tttcttgcgc tcgtcagaaa tggtgacctg attaatcacc acatcaatgc gtttagagtc
    21001 cagcgacgcc agcataccgt cccatttggt cggttttagt gacgcctcaa cgccaagatg
    21061 ttttgccagc tgttgggcaa attccacttc aaaaccggtt aatttgccgt catccccctg
    21121 aaaactgaac ggcggataag tgccttccag ccctaccagc agcgtgccgc tctctttgac
    21181 tttattaagc agaccttcat ctgcaaaact tttaacgctc atgcccgcaa ccagcgccac
    21241 ggccatcaca cccatcaatg cctgacgtcc cagatgtgct aatttcatat tcaccccgaa
    21301 tgttgttatg tctgtttgca gtgtagagcc attgtttgta aacacaaaaa caactccgct
    21361 acatcttatt cttatttaat atatatcaga agcaggcagg ctggaggatt tctgcacaag
    21421 tccggtacag gtttggcgct gatagtgttg atacttccgt aatgcgatgc ggtaattgtt
    21481 ggtgctggtg gtgggcagcc acggggcgag gatttcatca aggaaaccgt cctgcagctg
    21541 atcgagggaa atattttgct catgtagatg attacccaaa cggcgcagac gaacgacata
    21601 ttcacgcacc gtacctgggc tcatttcagt ttgctcaaaa agaaatttct taaagccgat
    21661 aatatcgaaa aaatcactct gcgttttgca atgtaaatca ccgcaaaatc ggcacaatgc
    21721 cgcccatgat tgttgttccg tttgccagcc tttttcatca agcagcgtgt ccaggcggga
    21781 aatctcgatt ttattcacta ttttgccgtc gcgaactaag gtaatgcgat cgagtaattt
    21841 acggcaatgc gcgcaatggg tctggctgtg tttaaagtct ttaagatagc ggcttaatgg
    21901 ccgtcttttc aggtgctgca ccatcattaa gaactcctgg tagtcaaagt taaagtgcgg
    21961 catttactga cgttataact tacccagttt agtgcgtaac cgtttaatgg cctggctgtg
    22021 taactgactg acccgcgatt ccccgacctc cagcaccgcg ccaatctctt tgagattcag
    22081 ctcttcctgg taatagaggg ttaataccag tttttcgcgc tccggcaacg tttcgatggc
    22141 ttccatcacc cgctggcgca gattactgtc cagcagttgt tgtagcgggt tttctcgctg
    22201 atgatcatca gtaaccagtt cgatgctatc gccgtgctct tcacgccact catcgtagga
    22261 gaagagctgg ctgttattgg tgtcgagcag catttgacga taatcggcaa tatcgatccc
    22321 taaacgttcc gctacctcag tttccgtggc gttgcggcca agttcctgct ccagttgccc
    22381 tattgcctgt gccacttcac gcgcgttgcg tcgcacgctg cgcggcaccc agtcacggct
    22441 gcgaagttca tccagcatag cgccacggat acgctgcact gcgtaagttg taaatgccgt
    22501 tccttgtagg gcgtcatagc gttcgacagc attaagtaac ccaatgccgc ccgcctgtag
    22561 cagatcgtca agttccacgc tcgcgggcag tcgaacctgc aggcgcaatg cttcgtgacg
    22621 caccagcggg acataacgct gccacagcga gtgtttatcc attacacctt cagcggtata
    22681 gagtgaattc acgataaaca gccctgcgtt atatgagtta tcggcatgat tatccgtttc
    22741 tgcagggttt ttaatcggac gattagtggg tgaaatgagg ggttatttgg gggttacgca
    22801 aaaagccgaa tgtaaaaaac cccgccggtg gcggggtttg agaggcattc agacgattaa
    22861 ccctgcagca gagacagaac ctgctgcggt acctggttgg cttttgccag cacggagtta
    22921 ccggcctgct ggataatctg cgctttcgac atgttggaca cttcggtcgc atagtcggcg
    22981 tcctgaatac gggactgcgc ttcagacagg ttggtagtgg tgttgttcag gttggtgact
    23041 gcggaatcca aacggttttg caccgcaccc agggaggagc ggaatttgtc gatggagctg
    23101 atggcttcat ccagagcttt caggggatcg gctgtcgtag ctgcttttgt ttcagcatct
    23161 gtcgtcaatt taccatcagc atctttataa actgccttac cgctgccgtt agtcactgaa
    23221 ccatcatcct gtaggtaaaa atctacagta tcagatgtgg tcaacgcctc gttgttatca
    23281 acataaaccg aatcaacacc ttttgttaca gcgccagttg tgccgttgac gctataacct
    23341 gcagtagtga aatcaacatt gtttcccgag gttgatacag ctttatcgaa agctgcctga
    23401 tcaacttttg cacctgttac tgaataagta atagtgtccg gcgtagtact attaccggta
    23461 aaggagatag aggtaccgcc aatgtcaatg gttgtcgctt tggtattatt ggctgcaagt
    23521 gtggacagac tagcctcagt caaagaagca ccagagccgt ttttcgttaa gcgcccagtt
    23581 gtatcaatgt agagtttatc tccattgctt gccgtaattt taccatcact gctgatctga
    23641 acatcctgtg cagaaccatc gatagtaata gtgcccttag cggtatcacc aactcccggg
    23701 gtcaaatatt tctgaacatc ggcagctgaa gccgttgtgg tatcaaaaga gtatgactta
    23761 cttgcactgt catacttgta attcgttgca ctagccgccg ttccgaagcc gttattaatg
    23821 gttgcctgaa cagtatcacc atcactaact ccagccagca aatcagacgc tttagcagca
    23881 tcgtaacccg cactcacagt atatttgttg ccgaccactg ttgcattagc agctaccaag
    23941 tcagctttag atgcagcagt attagccaca gccccgccac cattcacatt aaacccattc
    24001 agccccagcg tatcagagtc aattttcttc agatcaatag tgatagtctg gccgtcattc
    24061 gcaccaacct gaattttcat cgaaccgtct tttgccagta cgttcacgcc gttgaactgg
    24121 gtctgaccgg atacgcggtc aatttcgtcg agacgggatt tgatttcgtc ctgaatggag
    24181 tccagatccg aatcagagtt agtcccggta gaagcctgaa ccgtcagctc acggatacgc
    24241 tgtaagttgt tgttgatttc ggacagcgcg ccttcagtgg tctgtgcaac agaaataccg
    24301 tcgttggcgt tacgtgcagc ctgagtcagg cctttaatgt tagaagtaaa acggttagca
    24361 atcgcctgac ccgcggcgtc atccttcgcg ctgttaatac gcaagccaga agacagacgc
    24421 tcgatagaac tcgacagcgc agactggttc ttgttgatat tattttgagt gatcagcgag
    24481 aggctgttgg tattaatgac ttgtgccatg attcgttatc ctatattgca agtcgttgat
    24541 tacgttttgg gtttccaccc gtcggctcaa tcgccgtcaa ccctgttatc gtctgtcgta
    24601 aaacaacctt tagaattttt ttcacaaaca gccatttttt gttagtcgac gaaatactct
    24661 tttctctgcc ccttattccc gctattaaaa aaaacaatta aacgtaaact ttgcgcaatt
    24721 caggccgata accccagtat tcgttttacg tgtcgaaaga taaaaggaaa tcgcatggca
    24781 agtatttcat cgctgggagt cgggtcaggt ctggatttaa gttcaatcct tgatagcctc
    24841 accgccgcgc aaaaagcgac gctaacccct atttcaaatc agcaatcgtc gtttaccgct
    24901 aaacttagcg cctacggtac gctgaaaagc gcgctgacga ctttccagac cgccaatact
    24961 gcattgtcta aagccgatct tttttccgcc accagcacca ccagcagcac caccgcgttc
    25021 agtgccacca ccgcgggtaa cgccatcgcc gggaaataca ccatcagcgt cacccatctg
    25081 gcgcaggcgc aaaccctgac cacgcgcacc accagagatg atacgaaaac ggcgatcgcc
    25141 accagcgaca gcaaactcac cattcaacaa ggcgacgaca aagatccgat taccattgat
    25201 atcagcgcgg ctaactcatc gttaagcggg atccgtgatg ccatcaacaa cgcaaaagcg
    25261 ggcgtaagtg cgagcatcat taacgtgggt aacggtgaat atcgtctgtc agtcacatca
    25321 aatgacaccg gccttgataa tgcgatgaca ctctcggtca gcggtgatga tgcgctacaa
    25381 agttttatgg gctatgacgc cagtgccagc agcaacggta tggaggtctc ggtcgccgcc
    25441 cagaatgcgc agctgacggt caacaacgtc gccatcgaga acagcagcaa cactatcagc
    25501 gacgcgctgg aaaacatcac cctgaacctg aacgatgtca ccacgggcaa ccagacgcta
    25561 accatcactc aggacacctc caaagcgcaa acggcgatta aagactgggt gaatgcctat
    25621 aactcgctaa tagatacctt cagcagcctg accaaataca ccgccgtaga tgcgggagct
    25681 gatagccaga gttctagcaa tggcgcactg ctcggcgact ccacgctgcg gacgattcag
    25741 acgcagttga agtcgatgct gagtaatacc gtcagttctt ccagctataa aacgctggcg
    25801 cagattggta tcacgaccga tcccagcgat ggcaaactgg aactggatgc cgacaaactc
    25861 accgctgcac tgaaaaaaga tgccagcggc gtaggtgcat tgattgttgg cgatggtaaa
    25921 aaaaccggca tcacaaccac catcggcagc aacctgacca gttggctttc gacaacgggc
    25981 attattaaag ccgctaccga tggcgttagt aaaaccctga ataagttaac taaagactac
    26041 aacgccgcca gcgatcgcat tgatgcgcag gtcgcgcgct acaaagaaca atttacccaa
    26101 ctggacgttt taatgacctc gttaaacagc accagcagct acttaacgca gcagttcgaa
    26161 aacaacagta attccaagta atcattattc atcgggagac aggtcatgta cgcggcaaaa
    26221 ggcacccagg cctatgcaca aattggcgtc gaaagcaccg taatgagcgc cagccaacag
    26281 cagttggtca ccatgctatt tgatggagtg ctgagcgcac tggttagagc gcgactgttt
    26341 atgcaggaca acaatcagca aggcaaaggc gtctctttgt caaaagcgat caacatcatt
    26401 gagaacggac tgcgggtgag tcttgatgaa gagagcaaag acgaattaac ccaaaactta
    26461 attgctcttt atagctatat ggtcaggcgt ttgctgcaag ccaatttacg caacgatgtc
    26521 tccgcagtcg aagaggtgga agcattaatg cgtaatattg ccgatgcctg gaaagagtct
    26581 ttactctccc cttcttcgat tcaggaccca gtctgatgaa caatgcaccg catttgtatt
    26641 tcgcctggca acaactcgtc gaaaaaagcc agctcatgtt acgcctggca acggaagaac
    26701 aatgggacga actcatcgct agcgaaatgg cgtatgtgaa tgcggtgcag gagatcgcac
    26761 acctgactga agaggttgcc ccgtccacta cgatgcagga gcagctccgc ccgatgctgc
    26821 acctgattct cgacaacgaa agcaaggtaa agcagttatt acagattcgg atggatgaac
    26881 tggcgaaact ggtcggtcag tcatcggtgc aaaaatcggt gttaagtgcc tatggcgatc
    26941 agggcggctt tgtgctggct ccgcaggata acctcttttg aatcttaatg agccgatggc
    27001 tcgcgaataa tccgattacg gctacgcttc taatattccc cttgaatgga gtcgaagaat
    27061 gcgtaatccc acgctgttac aatgttttca ctggtattac ccggaaggcg gtaagctctg
    27121 gcctgaactg gccgagcgcg cagacggttt taatgatatt ggtatcaata tggtctggtt
    27181 gccgcccgcc tataaaggcg catcgggcgg ctattcggtc ggctacgact cctatgattt
    27241 atttgatcta ggtgagtttg atcagaaagg cagcatccct actaaatatg gtgataaagc
    27301 acaactgctg gccgccattg acgccctgaa acgtaatgac attgcggtgc tgctggacgt
    27361 ggtagtcaac cacaaaatgg gcgcggatga aaaagaagct attcgcgtgc agcgtgtaaa
    27421 tgctgatgac cgtacgcaaa ttgacgaaga aatcattgag tgtgaaggct ggacgcgtta
    27481 caccttcccc gctcgtgccg ggcaatactc gcagtttatc tgggatttca aatgttttag
    27541 cggtatcgac catatcgaac accctgatga agatggcatt tttaaaattg ttaacgacta
    27601 taccggagaa ggctggaacg atcaggttga tgatgaactc ggtaatttcg attatctgat
    27661 gggtgagaat atcgattttc gcaatcatgc cgtgacggaa gagattaaat actgggcgcg
    27721 ctgggtgatg gaacaaacgc aatgcgacgg ctttcgtctt gatgcggtca aacatattcc
    27781 ggcatggttt tataaagagt ggatcgaaca cgtacaggaa gttgcgccaa agccgctgtt
    27841 tattgtggct gaatactggt cgcatgaagt cgataaactg caaacgtata ttgatcaggt
    27901 ggaaggcaaa accatgctgt ttgatgcgcc gctgcagatg aaattccatg aagcatcgcg
    27961 catgggtcgc gactacgaca tgacgcagat cttcactggg acgctggtgg aagccgatcc
    28021 ttttcacgcc gtaaccctcg ttgccaacca cgacacccaa ccgttgcaag ccctcgaagc
    28081 accggtcgaa ccgtggttta aaccgctggc gtatgcctta attttgttgc gggaaaatgg
    28141 cgttccttcg gtgttctatc cggacctcta cggtgcacat tacgaagatg tcggtggtga
    28201 cgggcaaacc tatccgatag atatgccaat aatcgaacag cttgatgagt taattctcgc
    28261 ccgtcagcgt ttcgcccacg gtgtacagac attatttttc gaccatccga actgcattgc
    28321 ctttagccgc agtggcaccg acgaatatcc cggctgcgtg gtggtcatgt cgaacggaga
    28381 tgatggcgaa aaaaccattc atctgggaga gaattacggc aataaaacct ggcgtgattt
    28441 tctggggaac cggcaagaga gcgtagtgac agacgaaaac ggcgaagcga ccttcttttg
    28501 caacggcggc agcgtcagcg tgtgggttat cgaagaggtg atttaaatcc atccccggcg
    28561 gcaagccggg gagatttcat tacggcagtg gcgtcggtaa cggcgcttta tccagcgcgg
    28621 cggcacactc tgccgtcggg cgatccacac gctccatcgt cataccatcg tactcaatgg
    28681 tgttaccgtc acgctcaacc tcgtacagct cacgttttac ggtaacgtta gtgagatcat
    28741 cagacatcaa tgtcaactta cccggcacgg caatcccccg ctgccactga cggcaatcca
    28801 aggtatcgcc ctctttagtg acaatcaggc tgcctatcgc ttccggactg accagcgcac
    28861 gttgcggccc tttggtctgc cagtagcccg ccagccagtc cggcgcgggg gttttcacga
    28921 cattgttata gttttcgact tcggcacagc ccgccagcaa catgagcgcg cctgcaattg
    28981 cgagtttttt catcattcgt cctgcatgag aagaaaagag gattgtggca ttaaagctct
    29041 gatgtcgcca gcctttccag gttcagagag aaatttgatc taactattat ttttgtgaat
    29101 tattaccccg aaaataaatt tgcatggtgt atgattcgcg gctcttgttt caaaccttct
    29161 gagttcagag gctacattca tgtcatggca gcaattcaaa cacgcctggt tgattaaatt
    29221 ctgggcaccc atccctgcgg tcatcgcggc gggtattctc tccacttact attttggcat
    29281 tactggcacc ttttgggctg tcacgggtga atttacccgt tggggcggtc agctcctgca
    29341 gctgtttggc gtccatgctg aagagtgggg ttattttaaa attatccatc tggaaggatc
    29401 gccattaacc cgcatcgacg gcatgatgat cctcggtatg tttggcggct gctttgctgc
    29461 tgcgctgtgg gccaacaatg tcaaactacg catgccacgc agccgtatcc gcattatgca
    29521 ggccatcatt ggcggcatta tcgccggttt tggcgcgcgt ctggcaatgg gctgtaacct
    29581 ggcggcgttc tttaccggca ttcctcagtt ctcgctgcat gcctggttct ttgccatcgc
    29641 cactgccatt ggttcatggt ttggcgcgcg ctttaccctg ctgcccatct tccgtattcc
    29701 cgtgaaaatg cagaaagttt ctgccgcctc accgctgacg caaaaaccgg atcaggcgcg
    29761 gcgtcgtttt cgtctcggga tgctggtctt tttcggcatg ctgggctggg cgctgctcac
    29821 ggcgatgaac caaccaaaac tggggctggc aatgctgttt ggcgtcggct ttggtttact
    29881 gattgaacgt gcgcaaatct gctttacttc ggcgttccgc gatatgtgga tcaccggacg
    29941 tacccatatg gcgaaagcaa tcattatcgg tatggcggtg agtgccatcg ggatcttcag
    30001 ttacgtacag ttaggcgttg aacctaaaat catgtgggcg ggaccaaatg cggtaattgg
    30061 tggtttactt tttggttttg gcatcgtgct ggcaggcggc tgcgaaaccg gctggatgta
    30121 ccgcgcggta gaaggccagg tgcactactg gtgggtcggt ctgggcaacg tgatcggctc
    30181 aacgattctg gcgtattact gggatgattt cgctccggcg ctggccaccg actgggacaa
    30241 aatcaacctg ctgaaaactt ttggtccgat gggaggcctg ctggtgacat atttgctgct
    30301 gtttgctgcg ctgatgttga ttatcggctg ggaaaaacgc ttcttccgcc gtgaggcacc
    30361 gcagactgct aaggagatcg catgaaaaat atcgttcctg attaccgcct tgatatggtg
    30421 ggtgaaccct gcccttatcc ggcagtcgca acccttgagg cgatgccgca attaaaaaaa
    30481 ggtgagatcc tggaagtggt gagcgactgt ccgcagtcga tcaataatat tccactggat
    30541 gcgcgtaatc acggctacac ggtgctggat attcagcaag acgggccgac cattcgttat
    30601 ttaattcaga agtaatccct cattcccgct gtggcccaca gcgggaacat cgttacctct
    30661 gacacataga ttttacccgc cccctctcct attcttttca gtatgatctc aggaggttgc
    30721 tatgtgtgga cgctttgccc aatcccaaac gcgtgaggat taccttgcgc ttctcgcgga
    30781 agatattgaa cgcgatattc cctacgatcc cgaacccatt ggcagataca atgtcgcgcc
    30841 gggaaccaaa gttcttctgc tcagtgaacg tgatgaacac cttcatctgg atccggtttt
    30901 ctggggatat gctcccggat ggtgggataa accgccgctg attaacgccc gcgtagaaac
    30961 tgcctcaacc agtcgcatgt ttaaaccgct ctggcaacat ggtcgggcaa tctgttttgc
    31021 cgatggctgg tttgagtgga aaaaagaagg cgacaaaaaa agccttattt tatctatcgc
    31081 gctgacggac aacctgtttt tatggccgcg ataggtagca taccgtttga gcgtggtgac
    31141 gaagccgaag gatttttgat cgtcactgcg gcggcagatc aaggtctggt agatattcat
    31201 gaccgccgcc cgctggttct gtcgccagaa gctgcacggg aatggatgcg gcaagacatt
    31261 ggtggtaaag aagcctcaga aatcgctacc agaagctgtg tgccagcaaa ccagttcacc
    31321 tggcaccctg tgtcgcgcac ggtgggtaat gtcaaaaacc agggcgcgga gttaattcaa
    31381 cctgtttgtt gacatcctcc acgccctgaa tgacgaggac ccctgctacg ttcaggctgt
    31441 cgcctgaatc atttcggtgg gttcctgctt caacgagcgg cctgactgca ccatctctcc
    31501 acaggcaaga acggcgtgcc ccgccgctaa aatgttacga gctccattta catcggcgtt
    31561 cgctgtatat ccacatacct ggcatctgaa tttactttgt gacaggcgat tttctttcgc
    31621 tgtatgacca cagcacgcgc aacgctggct tgtgtacgct ggcggaacag caagcacctg
    31681 accgccacgc cagagctgct tgtactcaag ctggcggcgc atttcatacc agccctgatc
    31741 cagtatcgaa cggtttaaac ctgattttgc ccggacattg cgccccggct ggctgaccgt
    31801 acccgctgct gactttgaca tgttgctgac cttcaaatcc tcaatgacta tcattacgtg
    31861 gtttttgctg acggtcgttg tgactttgtg aaggtagtcc ctgcggatat ttgcgataca
    31921 ggaatgcagt cgctgtattt tgcgtttctg cttctgccag ttgttgctga atttgacctt
    31981 gcggcttaac tgtcgctgaa gtctcgccag cttcttctgg tttttctgaa aactgtttac
    32041 aggctcaaag actgtgccat ctgacagcga ggcaagttta gccacgccag catccagccc
    32101 gaccattgat gctgaagggt gaaccggagt tgatacttca ctttctgcct gaatactgat
    32161 gtaccactta ccgcaggact ggctgacagt gacatttttc acaacacccg tgacctgacg
    32221 gctgttccgg tagcgcatcc agccaagttt tggcagaaaa atacggctgt tttcctgagc
    32281 gagcttaacg ccctgcggat agcggaatgc atcattctgt caccgctttt tgaatcaggg
    32341 gaacgccgag cgcttctgga aaaagttttt gcaggcctgc tcagggtctt tcagtgactg
    32401 ttgcaatggc tgtgacggag catctttaat ccattgcgtt tcagtggcat ttttccactc
    32461 aaccaatcag gaagccattt tcgcgtaagg gatgtctttg ttaccggcct tatgattctc
    32521 attctgacgt gccagagtac gactgaaaac gaaacgacat gcgcctgcga agcgcctcat
    32581 ttcacgcgcc tgttgaccac cgggtcttaa ccggaatttg aatacctgta gtcgtttcac
    32641 agggcaaatt atactttggt ctgtgaaaaa aactgatatc cgacgcggaa ggcactgtgt
    32701 tttcctgatg catgctcacc gggtctttgt taccaagtac cgacgccaga tttttgacca
    32761 tgacgcgaca gaaaaactac gcacttactt ttcaaatgta tgtgcttatt ttgaagctga
    32821 actggttgaa atggatggcg aaccagatca cgtccatctg ttaattaact atccccccaa
    32881 actggcgata tccagtctgg taaacaaaca gcctcaaagg cgtatcgggt aggttactgc
    32941 gacgggatcg accagatatt gctgccaggt attaccgcaa agatgttttg tggcgtcccg
    33001 gctattttgc cagtagctgc ggaggtgcgc caatatccgt catccgccaa tacattgaac
    33061 aacagcaaac acctggtcag atgaaaaaac gagccttata tccccgccct gaaggacggg
    33121 cttttacggt gcactggata agcctggaga ttatctacac ctgcatggac atcattgtac
    33181 tatgccccgt acatgcccca tcacatcctc aacatcaaag tattagagcc ttgcaaacca
    33241 ttatgcgtct cagtttgtct catctatctt acgcaaaaaa aatagaacta atatcaagcg
    33301 gcgctatatt gcgtattaaa taaaaccacc tcaattacat taataaatct aaataaatat
    33361 catgcaaata atttccatgg ttatttttag gctatttaaa ttaaaaaaat aaattatgag
    33421 tcaatcataa ataacaacct ttaatataat ttgactacca aatttatatt cgttcacaaa
    33481 atagtcatgc attgtgtgca atagaaacag ttcagataaa aatagggatc tacttcacaa
    33541 tttaaatgag aaactaaaac ttacatcttg aaataatctc attgattgga tgaatatttg
    33601 tagtgcagtg acatcatttt taaataatag ttcaaataaa agggctcacg atgaaaaaat
    33661 taacagtggc aatttctgct gtagctgcat cagtactgat ggcgatgtct gctcaggcag
    33721 cagagattta caataaagac agtaacaagc tggatctgta cggaaaagtt aatgctaagc
    33781 actacttctc ttctaacgat gcagatgatg gcgacacaac ttatgttcgt ctgggcttca
    33841 aaggcgaaac tcaaatcaac gatcaactga ctggtttcgg tcagtgggaa tatgaattca
    33901 aaggcaaccg tgctgaatct caaggttctt ccaaagataa aacccgtctt gccttcgctg
    33961 gcctgaaatt cggtgactac ggctccatcg attatggccg taactacggt gtagcatacg
    34021 atatcggtgc atggaccgac gttctgccag aattcggtgg cgatacctgg acccaaacag
    34081 atgtgttcat gactggtcgc accacaggtg ttgcaaccta tcgtaacaac gacttctttg
    34141 gtctggttga tggtctgaac tttgctgctc agtatcaggg taaaaatgac cgcactgacg
    34201 taactgaagc taacggtgat ggtttcggtt tctccactac ttatgagtat gaaggattcg
    34261 gtgtaggtgc aacctatgct aaatctgacc gcactaatga tcaggttatc tacggtaaca
    34321 acagcctgaa tgcatctggt caaaatgctg aagtatgggc agctggtctg aaatatgatg
    34381 cgaacaacat ctatctggct accacctatt ctgaaaccca gaacatgact gtttttggta
    34441 ataaccatat tgccaacaaa gcacaaaact tcgaagtagt tgcacaatat cagttcgact
    34501 tcggtctgcg tccgtccgtt gcttacctgc aatctaaagg aaaagacttg ggtgcgtggg
    34561 gtgatcagga cctgattgaa tatattgatg taggtgcaac ctattacttc aacaaaaata
    34621 tgtccacttt tgttgattac aaaatcaacc tgattgataa gagcgatttc acgaaagcat
    34681 ctggcgttgc taccgatgat atcgttgctg taggattggt ttaccagttc taatctgatt
    34741 actcaaagat atgttgtggg aggctttgcc tccccaacat ataagtggcg ctctcaagcc
    34801 actttcctta gaagcactat cttgcttctg actatataaa ttacccttta ttttctggga
    34861 cgtttcaacg ccccattttt aataatactc agtgaacaat taacgtatca attagattta
    34921 ataacaacga tatccgtacc taacctgata tctaatgtca taagcatccc tttaattatc
    34981 ccctcaacct cttgtaaatt tcccactctt gagctccaca tagcccaaac gccggatgca
    35041 aaaaaaatca cacgtgcatc tctgaaattg tgatggagga agtgagtatc aattatatgt
    35101 gcttataaac gttcccgatt ggcacagaag tcaccggatt gttcaggctc caataggttg
    35161 agtatgtcgt atttattttc caaaataaaa atcgatgctg ttgctcctta ataaaatact
    35221 tttactctct ggacgctttt cttagctctc ttggtgttat attaaaacga ttatggaacc
    35281 tttcagtaaa acgagaagga cacttgtaac cattttccct ggcaatctcg ctgatgggtt
    35341 ttactgtcgt ttgtatagca ggacaacgca ttatttaacc tcacgtcgtc caatatactt
    35401 tgaaaactta ttccctcgct tgccagacgg cgatgcaatg tggaaacaga aatgtagaga
    35461 tatctcgcta ccttgtttgc tgtccatttt gttctaggtt cggatagcat taggttataa
    35521 caacgactta ttaatgattg tttactatct gataaaagta aacaattaac atgatgtgct
    35581 cctaatgaaa gtagaacgcc cattgctaag tgttcctgaa tttgcgttga gaagcctcgg
    35641 gaaatagatg tttttagttg ctcccaacaa tatattaact cggggttctg aggtaagcag
    35701 aaacctgtcg tgttacgaat ttgatcagtt atcgcataaa atttttgaaa cttttcaatt
    35761 agatcaatgg gataataaag catttctgca agataaagac ctgcttctgg ataattcaca
    35821 atataaaatt catatccaca gggaaataat attatctgat tattatcaac agttagacta
    35881 tgcgtctccc aattgataac tttttttccc tgacggatac gacataaagc tggcataaga
    35941 ggcttaaccc tgtgaatctc atgatgttta tgcatccgta tttcttcaat ctttaggtta
    36001 gacttatctc ttgccagcat actcttaccc tattttatct cataaactgg tgttatctca
    36061 gcggttgcaa ttttattggc attaagcata taaccaacta acgctccgct ggaattagaa
    36121 tccacaggaa tcttatcagt ttttagagcc catactttaa actggtaatg atggggttta
    36181 tctcctttgg gaggacatgc tccaccaaat cctgcataac caaaatcatt tcggccttga
    36241 acagcaccag tcggaagttt tgtaccatca cgtcttcctg catcagtggg caaatatgtt
    36301 acagttgctg gaatattagc aacagtccag tgccaccatc cactgcctgt aggagcatct
    36361 ggatcataca cagttacggc aaagcttttg gtgccttcag gagcaccaga ccaggttaat
    36421 gagggcgatg tattaccacc ttcacaccca aatccagaaa atacatgaga cgttgtaagt
    36481 tgctctcctg tttttatttc attactagtg acctgaaatg ctgcggcctg cgcagaaaat
    36541 gttatgaatg ccaatacact tgaaacgata agttttttca taaaaacctc attgttgtga
    36601 cctatcgtta ttttatttga tgtgccttga tctcattatg aataaaggcg caatgatcgc
    36661 gcaaatgcaa tcataattat aaaggtgccc cgaacattag ccataatatg cacaaatcag
    36721 attcctgtta tctcaaggag aattattttt taatgcggtg cacttcgtga caacaagtta
    36781 atgactaaca caccggcaca aatcaacatc atgcctatga tagctggcag gtccagtcgt
    36841 tgaccaaaaa atccccagga aagcaaacta atcagaacga taccgactcc tgaccagata
    36901 gcataagcaa tccctgtagg tatataagcc agcgtctgag ctaataacca gaatgatgca
    36961 caatagcaaa taattgtgcc aacagagggc cataaccgtg taaacccctc tgaaaacttc
    37021 attaaggttg taccaatgac ctctgcaagt attgcgccac caagataaat ataagggttc
    37081 atagcatatt ctttcctgtt catactggag agaattgtac tacagtttga acacaactca
    37141 ccgttttcat cctggtgtat gtgtatccaa ggggctctgg cgtggcaaga gataaaggca
    37201 ggaacaataa tgatcagaag gaaccccagg ctaacgaatg agttcactct tcctgggagc
    37261 aaagcgaaga caaaacatgt tatcgcgttt acggatcgtg gatggcagaa aacaaccagg
    37321 atcaggtatc cattttaaac cagaaattga gtgagtttgc cccatccatg ccccacgctg
    37381 tgggatcgga tataattaaa caagcatagt caacaggtta cacaacctac acctgcatgg
    37441 acatcacttc ctgatacgcc gccaccagct tattacgcac ctgaatcccc atttgcatag
    37501 aaactgaggc tttttgcata tcggtcatca catcgtttag cgccacgccg ggttcaccga
    37561 gggtgaattt ttctgcctgc gtgcgggcga ctgtttgtgt atcgcttatg cgatcgagcg
    37621 cggcgtgcag ctgcccggca aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg
    37681 cactcatcgc cgtagcctgt aactggctga taaccccttc aatcccctgt atcgctgaca
    37741 ttttcgtctc ccggataatt tctggtaaca aagcctacca gtaagtcaat aagacaaagg
    37801 cgctaaatag caacaaaaaa actggtttat tggcggatag aaaaaacgaa agcacaaata
    37861 atgggagcgt caatttttcg agtttgctga cccgagagtg agtcttgttc cactttgcca
    37921 ataacgccgt ccataatcag ccacgaggtg cgcgatgaat gcgactgcag cccagacaaa
    37981 atctcttgag tggcttaatc gcctgcgtgc gaatccgaaa attccattga ttgttaccgg
    38041 ttccgcggca gtggcggtca tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg
    38101 cacattattc agcaatcttt ccgatcagga tggtggcgca attgtcagcc aactgacgca
    38161 aatgaatatt ccttaccgct tcagcgaagc cagcggcgct attgaggttc cggcagataa
    38221 agttcacgaa ctgcgcctgc gcctggcgca acaaggtttg cccaaaggcg gcgcggtcgg
    38281 tttcgaactg ctcgatcagg aaaagtttgg tatcagccag ttcagcgaac aggtgaatta
    38341 tcagcgggcg ctggaaggcg agctttctcg taccatcgaa actatcggcc cggtaaaagg
    38401 ggcgcgcgta catctggcaa tgccgaaacc gtctttattc gtccgtgaac aaaaatcccc
    38461 ttctgcatcg gtgacggtaa atctgttacc cggccgcgca ctcgatgaag ggcaaattag
    38521 cgctattgta catctggttt ccagcgcggt tgctggtctg ccgcctggaa acgtcacgct
    38581 ggtggatcag ggcgggcatc tgttaaccca gtccaatacc agcgggcgcg atcttaatga
    38641 cgctcagttg aaatatgcca gcgatgtcga aggccgtatt cagcagcgta ttgaagcgat
    38701 cctgtcacct attgttggta acggtaatat tcacgcctag gtcacggcgc aactggactt
    38761 cgccagtaaa gaacaaacgg aagaacagta tcgccctaac ggtgatgaat ctcatgcggc
    38821 gcttcgttca cgccagctta atgagagcga gcaaagcggt tccggttatc cgggcggcgt
    38881 accgggggcg ttgtcgaatc aaccggcacc tgcgaataac gcgccaatca gcacgcctcc
    38941 gacaaatcaa aataaccgcc agcagcaggc gagcaccacc agcaatagtg ggccgcgtag
    39001 cacacagcgg aatgaaacca gtaactacga agtcgatcgc accattcgtc ataccaaaat
    39061 gaacgtgggc gatgtgcaac gtctgtcagt cgcggtagtg gtgaattaca aaaccttgcc
    39121 agacggtaaa ccattgcctc tcagcaacga acagatgaag caaattgaag ctctgacccg
    39181 cgaggcgatg ggcttttctg aaaaacgcgg tgattcgctc aatgttgtta actcgccgtt
    39241 caatagcagt gacgaaagcg gcggagcact gccgttttgg caacagcaag tgtttatcga
    39301 tcagttgctg gctgccggtc gctggttgct ggttgctggt actgctggtg gcgtggttgc
    39361 tgtggcggaa agcggtacgc ccgcagctaa cacgtcgcgc cgaggcggtg aagactgtgc
    39421 agcaacaggc gcaggcccac gaggaagtgg aagatgcggt ggaagtccgt ctgagcaaag
    39481 acgaacaact ccaacaacgg cgcgctaacc aacgactggg ggcagaagtc atgagccagc
    39541 gtatccttga aatgtctgat aacgatccgc gcgtggtggc gctggtcatt cgccagtgga
    39601 taaataacga tcatgagtaa cctgacaggc accgataaaa gcgtcatcct gctgatgacc
    39661 attggcgaag accgggcggc agaggtgttc aagcacctct cccagcgcga agtgcaaacc
    39721 ctgagcgctg caatggcgaa cgtcacgcag atctccaaca agcagctaac cgatgtgctg
    39781 gcggagtttg agcaagaagc tgaacagttt gctgcactga atatcaacgc caacgattat
    39841 ctgcgttcgg tattggtcaa agctctgggt gaagaacgtg ccgccagcct gctggaagat
    39901 attctcgaaa cccgcgatac cgccagcggt attgaaacgc tcaactttat ggagccgcag
    39961 agcgccgccg atctgattcg cgatgagcat ccgcaaatta tcgccaccat tctggtgcat
    40021 ctgaagcgcg cctaggccgc cgatattctg gcgttgttcg atgaacgtct gcgccacgac
    40081 gtgatgttgc gtatcgccac ctttggcggc gtgcagccag ccgcgctggc cgaactgacc
    40141 gaagtactga atggcttgct cgacggtcag aatctcaagc gcagcaaaat gggcggcgtg
    40201 agaacggcag ccgaaattat caacctgatg aaaacgcagc aggaagaagc cgttattacc
    40261 gccgtgcgtg aattcgacgg cgagctggcg cagaaaatca tcgacgagat gttcctgttc
    40321 gagaatctgg tggatgtcga tgatcgcagc attcagcgtc tgttgcagga agtggattcc
    40381 gaatcgctgc tgatcgcgct gaaaggggcc gagcagccac tgcgcgagaa attcctgcgc
    40441 aatatgtcgc agcgtgccgc cgatattctg cgcgacgatc tcgccaaccg tggtccggtg
    40501 cgtctgtcgc aggtcgaaaa cgaacagaaa gcgattctgc tgattgtgcg ccgtcttgcc
    40561 gaaactggcg agatggtgat tggcagcggc gaggatacct atgtctgata atctgccgtg
    40621 gaaaacctgg acgccggacg atctcgcgcc accaccggca gagtttgtgc caatggtcga
    40681 gtcggaagaa accattattg aagaggccga acccagcctt gagcagcaac tggcgcaact
    40741 gcaaatgcag gcccacgagc agggttatca ggcggggatt gccgaaggtc gccagcaagg
    40801 tcacgaacag ggctatcagg aaggactggc ccaggggctg gaacaaggtc tggcagaggc
    40861 gaaggctcaa caagcgccaa ttcatgcccg gatgcagcaa ctggtcagcg aatttcaaac
    40921 tacccttgat gcgcttgata gtgtgatagc ttcgcgcctg atgcagatgg cgctggaggc
    40981 agcacgtcag gtgatcggtc agacaccaac ggtggataac tcggcactga tcaaacagat
    41041 ccaacagttg ttgcagcaag aaccgttatt cagcgggaaa ccacagctgc gcgtacaccc
    41101 ggatgatctg caacgtgtgg atgatatgct cggcgcaact ttaagtttgc atggctggcg
    41161 cttgcggggc gatcccaccc tccaccctgg cggatgtaaa gtctccgccg atgaaggcga
    41221 tctcgacgcc agtgtcgcca ctcgctggca agaactctgc cgtctggcag caccaggagt
    41281 ggtgtaatga ccacgcgcct gactcgctgg ctaaccacgc tggataactt tgaagccaaa
    41341 atggcgcagt tgcctgcggt acgtcgctac gggcgattaa cccgcgctac cgggctggtg
    41401 ctggaagcca ccggattaca attgccgctc ggcgcaacct gtgtcattga gcgccagaac
    41461 ggcagcgaaa cgcacgaagt agaaagcgaa gtcgttggct ttaacggtca acggctgttt
    41521 ttaatgccgc tggaggaagt ggaaggtgtc ctgcccggcg cgcgtgtata tgccaaaaac
    41581 atttcggcag aagggctgca aagcggcaag cagttgccgc tcggtccggc gttattaggt
    41641 cgtgttctgg acggtagcgg taaaccgctc gatggcctgc ccttccccga tacgacggaa
    41701 accggtgcgc tgattacccc gccatttaac ccgttgcaac gtacaccgat tgaacatgtg
    41761 ctggacaccg gcgtgcgccc aatcaatgcc ctgcttaccg tcggacgcgg gcagcgtatg
    41821 gggctgtttg ccgggtcggg cgttggtaaa agtgtgctgc tggggatgat ggcacgctac
    41881 acccgcgccg atgtcattgt cgtgggtttg attggtgaac gtgggcgcga agtaaaagat
    41941 tttattgaga atatcctcgg tgccgaagga cgtgcacgct cagtggtgat tgccgctccg
    42001 gcggatgttt ctccgctcct gcgaatgcaa ggtgccgcct atgccacgcg cattgccgaa
    42061 gattttcgcg atcgtggtca gcatgtgttg ctgattatgg actccctcac ccgctacgcg
    42121 atggcccagc gtgaaattgc gctggcgatt ggcgaaccac ctgccactaa aggttatcca
    42181 ccgtcggtgt ttgccaaatt accggcactg gtcgagcgtg ccggtaatgg cattagcggc
    42241 ggcggctcga ttaccgcgtt ttataccgtg ctcaccgaag gcgatgacca gcaggacccc
    42301 attgctgact ccgcgcgggc aatcctcgac ggtcacattg tgctgtctcg ccgactggcg
    42361 gaagccgggc actatccggc tatcgatatt gaagcgtcga tcagtcgcgc aatgacggcg
    42421 ttgatcagtg agcaacatta cgcgcgagtg cgcaccttca aacagctgtt gtcgagtttt
    42481 cagcgtaacc gcgatctggt tagcgtcggc gcgtatgcca aaggcagcga tccgatgctc
    42541 gataaagcca tcgccctgtg gccgcagctg gagggctatt tgcaacaagg catttttgaa
    42601 cgcgcggact gggaagcgtc tctccagggg ctggagcgta ttttcccgac agtgtcataa
    42661 cccaggagat aacggcagat ggcggaagaa cagctcaaaa tgttgatcga ttatcagaat
    42721 gaatatcgca ataacctcaa tagcgatatg agtgccggga tgaccagcaa ccgctggatc
    42781 aactatcagc agtttatcca gacgctggaa aaagccatta ctcagcatcg ccagcaactt
    42841 aatcagtgga cgcagaaagt tgacattgcc ctgaacagct ggcgagaaaa gaaacaacgt
    42901 ttgcaggcct ggcagacact gcaggaacgg caatccacgg cggcactgct tgcagaaaac
    42961 cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg ccgccatgag gaaacctgaa
    43021 tgattcgctt agcgcccttg attaccgccg acgttgacac caccacattg cctggcggca
    43081 aagccagcga tgctgcacaa gattttctca cgttgttgag cgaagcatta gcaggcgaga
    43141 caacaactga caaagcggcc ccccagttgc tggtagcaac agataagccc acgacaaaag
    43201 gcgagccgct ggtcagcgat attcttgccg atgcgcaaca agcggattta ctgatccctg
    43261 tggatgaaac acttcctgtc atcaacgacg aacaatccac atcaacaccg ttaaccaccg
    43321 ctcagacgat gacgttggct gcggtggctg acaaaaatac gacaaaagac gaaaaagcgg
    43381 atgatcttaa tgaagacgtc accgcaagtc tgagcgccct ttttgcgatg ttgccgggtt
    43441 ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt gttaccggca gagaaaccaa
    43501 cgctcttcac aaaactgact tctgcgcaac tcactacagc acagcctgat gacgcccccg
    43561 gcacaccagc tcagccatta acaccgctgg tagcagaagc ccagagtaaa gcggaagtca
    43621 tcagcacgcc ttcgccggtg accgctgacg ccagcccgct aatcactcca caccagacac
    43681 agccactgcc caccgtcgcc gcgcctgttt tgagtgtacc gctgggttct cacgaatggc
    43741 aacaatcatt gagccagcat atttcgctgt tcacccgcca ggggcaacaa agtgcagagt
    43801 tgcgtctgca cccacaggat ttaggtgaag tgcaaatttc cctcaaagtg gatgataacc
    43861 aggcgcagat ccagatgatt tcaccgcatc agcacgtacg cgccgccctg gaagcagcgc
    43921 tgccggtact gcgtacgcag ctggccgaaa gtggcattca gttagggcaa agcaacatta
    43981 gtggcgaaag ctttagtggt cagcagcagg ccgcttccca gcaacagcaa agccaacgca
    44041 cagcaaacca tgaacctctg gcgggggaag acgacgatac gcttccggtt cccgtctctt
    44101 tacaagggcg tgtaacaggt aacagcggcg ttgatatttt cgcctaacgt cagaggtagc
    44161 accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag acgcaggata attagccgat
    44221 aagcagtagc gacacaggaa gaccgcaaca catgactgat tacgcgataa gcaagaaaag
    44281 caagcgatcg ctttggatcc cgattctggt attcattacc ctcgcggcct gtgccagcgc
    44341 aggttacagc tactggcatt cgcatcaggt tgccgctgac gacaaagcgc agcaacgcgt
    44401 cgtgccctcc ccggtcttct atgcgctgga tacctttacg gtcaatttgg gcgatgcgga
    44461 tcgcgtgctt tatatcggca taaccctgcg actgaaagat gaagcaaccc gctcgcggct
    44521 gagtgagtat ttgccggaag tccgtagtcg cttgctgtta ctgttttcac gtcaggatgc
    44581 tgccgtactg gcgacagaag aaggcaagaa aaacctgatt gccgagatta aaaccacact
    44641 ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc accgacgtgc tgtataccgc
    44701 ttttattctg cgataacgac atgggcgata gtattctttc tcaagctgaa attgatgcgc
    44761 tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc cagtgttagc ggcgaaagtg
    44821 acattcgtcc gtacgatccg aatacccaac gacgggttgt gcgcgaacgt ttgcaggcgc
    44881 tggaaatcat taatgagcgc tttgcccgcc attttcgtat ggggctgttc aacctgctgc
    44941 gccgtagccc ggacattacc gtcggggcca tccgcattca gccgtaccat gaatttgccc
    45001 gcaacctgcc ggtgccgacc aacctgaacc taatccacct gaaaccgcta cgcggcacag
    45061 ggctggtagt gttctcgccg agtctggtgt ttatcgccgt ggataacctg tttggcggcg
    45121 atggacgctt cccgaccaaa gtggaaggtc gcgagtttac ccatactgaa cagcgcgtca
    45181 tcaaccgtat gttgaagctg gcgctggaag gctatagcga cgcctggaaa gcgattaatc
    45241 cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgga atttaccaat atcaccacct
    45301 cgccgaacga cattgtggtt aacacgccgt tccatgtgga gattggcaac ctgaccggcg
    45361 aatttaatat ctgcctgcca ttcagcatga tcgagccgct acgggaattg ttggttaacc
    45421 cgccgctgga aaactcgcgt aatgaagatc agaactggcg cgataacctg gtgcgccagg
    45481 tgcagcattc acagctggaa ctggtggcca actttgccga tatctcgcta cgcctgtcgc
    45541 agattttaaa actgaaaccc ggcgacgtcc tgccgataga aaaacccgat cgcatcatcg
    45601 cccatgttga cggcgtcccg gttctgacca gtcagtacgg tactctcaac ggtcagtatg
    45661 cgttacggat agaacatttg attaacccga ttttaaattc tctgaacgag gaacagccca
    45721 aatgagtgac atgaataatc cggccgatga caacaacggc gcaatggacg atctgtgggc
    45781 tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc gctgccgacg cggtgttcca
    45841 gcaatttggc ggtggtgatg tcagcggaac gttgcaggat atcgacctga ttatggatat
    45901 tccggtcaag ctgaccgtcg agctgggccg tacgcggatg accatcaaag agctgttgcg
    45961 tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg ggcgaaccgc tggatattct
    46021 gatcaacggt tatttaatcg cccagggcga agtggtggtt gttgccgata aatatggcgt
    46081 gcggatcacc gatatcatta ccccgtccga gcggatgcgc cgcctgagcc gttagtgatg
    46141 aataaccatg ctactgtgca atcttccgcg ccggtttctg ctgagccact gctgcaggtg
    46201 agcggcgcac tcatcgccat tattgccctg atcctcgctg ctgcctggct ggtaaaacgg
    46261 ctgggatttg cccctaaacg cactggcgtt aacggtctga aaattagcgc cagtgcttcg
    46321 ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag atgcacggct ggtgctcggc
    46381 gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc cttcagcacc aacggaagag
    46441 ataccgcaga ccgattttca gtcggtcatg aaaaatttgc ttaagcgtgg cgggagatcc
    46501 tgatgcgtcg tttgttttct gtcgcaccgg tccttctctg gctgattacg cccctcgcct
    46561 tcgcgcaact gccgggtatc accagccagc cgctacctgg cggtggacaa agctggtcgc
    46621 tcccggtgca gacgctggtg ttcatcacct cgttgacgtt cattccagcg attttactga
    46681 tgatgaccag tttcacccgc atcattattg tttttggttt attgcgtaac gcgctgggaa
    46741 cgccctccgc gccacctaac caggtattgc tggggctggc actgtttttg acctttttta
    46801 ttatgtcgcc agtgatcaac aaaatttatg tcgatgcgta ccagccattc agcgaagaga
    46861 aaatctcaat gcaggaggcg ctggaaaaag gggcgcagcc gctgcgtgag tttatgctgc
    46921 gtcagacccg cgaggcagat ttagggttgt ttgccagact ggcgaatacc ggcccgttgc
    46981 aggggcctga agctgtgccg atgcgcattt tgctcccggc ctacgtgacc agcgagttga
    47041 aaaccgcatt tcagataggc ttcacgattt tcatcccttt tttgattatc gacctggtga
    47101 ttgccagcgt gttgatggca ttggggatga tgatggttcc ccccgccacc attgctctgc
    47161 cctttaaact gatgctgttt gtactggtgg atggctggca attgctggtc ggttcgctgg
    47221 cgcagagctt ttacagctag agaggcaaaa tgacacctga atcggtcatg atgatgggaa
    47281 ctgaagcgat gaaagtcgcg ctggcactgg cagccccgct gttgctggta gcgctggtca
    47341 cgggccttat catcagtatt ttgcaggcag ccacgcagat taacgaaatg acgctgtcgt
    47401 ttattccgaa aatcatcgcc gtatttatcg ccatcattat tgccggaccg tggatgctca
    47461 atctgttgct ggattacgtc cgcaccttgt tcactaacct gccgtatatc atcgggtagc
    47521 ccgtacaatg atgcaggaga caagcgatca atggctttcc tggttaagcc tgtacttctg
    47581 gccgttactg cgcgtgctgg cgctcatttc taccgcgcca attctgagcg aacgcagcgt
    47641 gccgaaacgg gtaaaactgg gtctggcaat gatgatcacg ttcgccattg ccccatcatt
    47701 acctgccaac gatgttcctg ttttttcgtt ctttgctctg tggctggccg tacagcagat
    47761 cctgatcggc attgcgcttg gttttaccat gcaatttgcc tttgccgctg tgcgaaccgc
    47821 tggcgaaatt atcggtctgc aaatggggct gtcatttgcg acatttgtcg atcctggcag
    47881 ccatctcaat atgcccgttt tagcgcgcat catggatatg ctggcattac tgctgttcct
    47941 gacatttaac ggtcatttat ggttgatttc actgctggtc gatacctttc acaccctgcc
    48001 gattggtagc gaaccgttga acagcaatgc gtttctggca cccaccaaag cagggagttt
    48061 gattttcctt aacgggctga tgctggcgtt accgctcatt actctgttgc tgaccctgaa
    48121 tctggcatta ggtttactga atcgtatggc cccgcaatta tccatttttg ttattggatt
    48181 tccattaact ctgactgtcg gcatctcttt aatggcggca ttaatgccgt taattgcgcc
    48241 tttttgcgaa catttattca gtgaaatttt taatttgctg gctgatatta ttagtgaatt
    48301 accattaata taattccgta acgtttatca tgctacccta aggattatcc gaaaaataat
    48361 atctgcgaac atcttccagg atactcctgt agcgaaatat ttgttttaag ctcactcaca
    48421 tatcgcaaca tttactttaa gacaattcca ggcaaattat acaacacttt acgggatagt
    48481 aagtccgcct gaaaaattgc gagagtggcg cattaggtga cccatgttgt tccgtttagt
    48541 catgatgaaa tattcaggta aggggaatta tcgttacgca ttgagtgagg gtatgccatg
    48601 tcaacgatta ttatggattt atgtagttac acccgactag gtttaaccgg gtatctgttg
    48661 agtagagggg ttaaaaaaag agaaatcaac gacattgaaa ccgttgatga ccttgccata
    48721 gcttgtgatt cacagcgtcc ttcagtggtg tttattaatg aggactgttt catccacgat
    48781 gcttctaaca gtcagcgcat caagcacatc attaatcaac atcccaatac gttatttatc
    48841 gtttttatgg caattgccaa tgttcatttt gatgaatatc tattggtcag aaaaaattta
    48901 ttgatcagtt ctaaatcgat taaaccggaa tctctcgacg atatccttgg cgatattctg
    48961 aaaaaagaga caacgataac ctcgttttta aatatgccga cgttatcatt gagccgaacc
    49021 gaatcgagta tgttgcgaat gtggatggca ggtcagggaa ccattcaaat ctccgaccaa
    49081 atgaatatca aagccaagac cgtttcatcg cataaaggta atattaaacg taagatcaaa
    49141 acgcataata aacaggttat ctaccatgtc gtccgactga cggataatgt gactaatggt
    49201 atttttgtca acatgcgcta acacattctg actggtggtt tcccaccagt caggctgaat
    49261 aagattactc tgctttctca acaaagatac cgtcctgatg ccctgcttca ttaaagaacc
    49321 agatgccgag cgggtagtct tccagcgcaa ccaggtacat tgtgccttca ctaaactcct
    49381 caactgccag taccactcca ggacgacgcg gaccgccatc cgttttgact gttacccgat
    49441 cattcacctt catcgttttc ctcctgtggc tttgtgccag tgtagaacaa tttcgttttt
    49501 ctggcagcgc caagcgcgcg cgagtgctga ttttctcgac ggtctatact taagagatgc
    49561 caggcggact taacgactgg cggcaacaac agagtaacgg ttgcgaggaa agatgatgaa
    49621 aaccgcaaaa gtgtacagcg ataccgcaaa acgtgaggtc agcgtcgatg tcgatgccct
    49681 gctggcggcg atcaatgaaa ttagcgaaag cgaagttcat cgcagccaga acgattctga
    49741 acacgttagc gtcgatggac gtgaatatca tacatggcgt gaattggcgg atgccttcga
    49801 actggatatt catgacttca gcgtctctga agtgaatcgt tgaatgcaca ataaaaaaat
    49861 cccgaccctg agggtgtcgg gatgaaactt gcttaagcaa gaagcactta aaaaattcgt
    49921 tacaccagga aatctgatgt gttcatcacc ttatctgcaa tttttttcgc tgacaagaaa
    49981 atattcgcgc tatgaatgat taacgctact tatgaataga aatgtgacgc gcgtcacccc
    50041 ttggtggctc tggtgtcaac gggatcgctc ccgaaaaaat catctttgat tatttgtgct
    50101 gttttgagaa tcatcctggt aaggggagta ctgcgcgacc atgttttcaa ttcaacaacc
    50161 actactggtt tttagcgatc ttgatggcac cctgctggac agtcatagtt atgactggca
    50221 accggcagcc ccctggctca gccgtttaca cgaagccaat attcccgtca ttctctgtag
    50281 cagtaaaact ttagcggaaa tgctgtactt gcaaaaaatg ttggggctac aaggcttacc
    50341 gctgattgca gagaatggcg cagtgatcca gcttgctgaa caatggcagg atatagacgg
    50401 ttttccacgc atcatctcag gtattagcca tggcgaaatc tgccaggttt taaatacgct
    50461 acgcgagaaa gaacatttta aattcacgac ttttgatgat gtcgacgatg caaccattgc
    50521 cgaatggacg ggattaagcc gtagctaggc ggcgctgacg cagctgcatg aggcgtcggt
    50581 aacgctaatc tggcgcgaca gtgacgagcg tatggcacaa tttatcgctc gtctgaacga
    50641 actgggcttg cagtttatgc agggtgcgcg cttctggcac gtactggatg cctctgccgg
    50701 aaaagatcag gctgccaact ggattatcgc gacctatcaa caattgtcag gcagacgccc
    50761 aaccacactt ggcctgggcg atgggccaaa cgatgcgcct ttactggagg taatggatta
    50821 cgcggtgatt gtgaaagggc taaaccgtga aggggtacat ctgcatgatg aggatccggc
    50881 ccgcgtctgg cgaacgcagc gtgaaggacc ggaaggctgg cgtgaagggc tggaccattt
    50941 tttctccgcc cgttaagcgt tatcgctcgc gaatacccga ttgcgcccgg cctgttttgc
    51001 gaggtaaagc cgacggtcgg ccaatgactg gagttgttca aaatcataat caccggtttc
    51061 ctcgctgcta cttaccccca gcgaggcact gatgcgtatc gttgtactct tggcgatcag
    51121 catctctttt tcatttaact tcaggcgaat acgttctgcg acctccgcag cctgcgtcag
    51181 attcgcgcct ggcagaatca cacaaaactc ctcaccaccg acccgcccgg caacgtcctg
    51241 cgcacgcaag gaactgctaa ttaatccggc agcatgagaa agaacacggt cgcccgcctg
    51301 atgaccaaag cggtcattaa tcgctttaaa atggtcaaga tcgacctgaa tgacagaaaa
    51361 aggatgttgg tgcgtctgac acaatttagc gagcggacgg gctttttcga acagtgcgcc
    51421 acggttatat aaacgcgtta aggtgtcgtg ccacgcctgc cactgcaacg agctttgcag
    51481 aacatacatg ttgctgacca tccggcgaat cacataccag gagaggagta acatggtggt
    51541 aaagagcgcc cacagcaggg ttaatgcaat gctgatactg ccgaaatcgc cgcgcacgcc
    51601 ttcgcttagc gtatggacgc gcgccagcac accgtcgaaa tggtccagac gttcccagct
    51661 aacatagcga ctgtccatac gaatgccgcc ccgcgtgtca tgttccattg cctgcgccag
    51721 caaggccagt tcacgaggat caaaaatatt ccctgttgga tggtcaggat tggaagaggt
    51781 caaaaatctc agcttactgt catagagctg atactcacca tcgaggtttt tatcgatggc
    51841 gtttcttaaa aattgctgca tagtacgcac gggaatactc atccccagca cgccatacca
    51901 gtagttatta ctgtctaccg gaacactgac ggtaacctgc ggttcagtat tgctggcgtg
    51961 ttccggttgc gaagtgaacc agcgtaccgc gcggtgacga ttttctcgtt gcgaatggcc
    52021 gataaaccag ggttgggtga catagccgta ataacgcgtt ggtacattgc gcgtaaacaa
    52081 ggtcggctgc gtcgaaacgt aaaatccagc acgcgagaca tacatcgcct gttcaaccat
    52141 cgacgaggag ttgtgcgcca gtcgcagcaa gtaaccaact tccagtgcag cggtaatttc
    52201 attgtcgagg ctttcatttt cgcgagacag gaaattcccc tcgctgacta atgcatccga
    52261 tacgccattg actggcaggg tgcgccgtcg gttgagttcg atttgccagg cgtgctcgtc
    52321 gcgatgctgt tcgaactcgg ttacggcatt acgcagagag gtgaaatcca gtggcgccac
    52381 gagagcttcg cgcatgccat tacgcaaaaa gatcagtttg tcgacgttat actgcaaatg
    52441 cttatcgagc gcgttggcta cgttttccag atgattacgc tagctggaga tataggcatc
    52501 ttccagcacc accacttcac gccaggtgag caaggtggaa aaaagcaata ccacgataaa
    52561 gcagagatta acgacatgac cgggccccag gcggcgtgcg agttttttca accagctctg
    52621 gttttccatt tttgtctcgt gctgcaccct gacgactccc aacctgcttt tttatgattc
    52681 tggcataacc cggcaatagc gtaccgcaaa acagcgataa ttttataatg gtggcatgat
    52741 aagacgcgac tatcattcat tgcaggcatt ggaacaccag gccggatgtg atccggcctg
    52801 gtgttaatga taccctttca aacttcttcc gcagaacgac tcccctcccc cggaacaaga
    52861 tcggtttcat gaaaagcttc ccgcttgacc ccgtaaccgt cataccaacg acactcaacc
    52921 ataccgctgg agtatccagt gacaatcatc cgcgggccgc cctctttaac cgtaacttcc
    52981 tcactaacca taaagctcat actcgcctcc ttttttcgag tgaaacttgt tcaccttagt
    53041 tgaagatggc gaattttgca taatagccag tgcgagtatt aatgtgcctg accgcgcatt
    53101 ttcgctactg ctttcactcc cagcaccacg atgccgccga tgataaatcc aagaatcaga
    53161 tttaaaacag tcggtaatat catcgccacc actgcacttt gctgcccggc gatatgttca
    53221 atagcgtgat gcagcggcgc aataccatgt accacaatcc cgccgccgac gaggaacatc
    53281 gccagcgtgc cgacaatcga taatgctttc atcagccaag gcgcgataat caataaccct
    53341 ttacctaatg cctgcatcag cgcgctagat ttttccgcca gccaataccc taggtcatca
    53401 atcttaacga taacccctac cagaccgtag acgcccacgg tcactaccag cgcgatgcct
    53461 gaaagcacca gcacctgatt aagcaacggc gcctctgcca caatccccag cgtgatggcg
    53521 acgatttccg cagacaaaat aaaatcggta cgaatcgccc ctttaatttt gtccttttca
    53581 aacttcagcg gatcctgcgc cgccagcttc tccagacgct gctggctctg cgctggatct
    53641 tctttatgtt tacgcgcctc cagcatatgc agcactttct ctaccccttc aaagcagaga
    53701 aacgcgccac caatcatcaa cagtggcgta atcgcccacg ggataaacgc actgatgatc
    53761 agcgccagcg gcaccagaat cactttattg atcagcgatc ctttcgccac gccccagacc
    53821 acgggaagtt cccggttggc ccgcacgcct gaaacttgtt gcgcattgag cgataaatca
    53881 tcccctaata caccggcggt tttcttcgcc gccagtttgc ccatcacaga gatatcgtcc
    53941 agcagtgtgg cgatatcatc gagcaacgtc agtaaactac tcccggccaa aagcattccc
    54001 tctcattttt attgttgaag ggtgtaagta tgaagcaaaa acggcagctc tgatacttgc
    54061 ttcacaggtc aacatttatt taacaatcaa gtgcaattat atctctcgcc agcagaatga
    54121 ttttcacgtt tactatgaac caccttttta tttccgctgt gagggattat gcgtttccgc
    54181 cagttgttac cgctttttgg cgcgctgttt gcgttgtata tcatttgggg ctcaacctat
    54241 tttgtcattc ggattggcgt gggaagctgg cctccgttaa tgatggcggg cgttcgattc
    54301 caggcagccg gtattttatt gctggcattt ttgctactgc gcggacacaa acttcccccg
    54361 ctacgtccgc tgctcaatgc cgcgctgatt ggcctgttat tgctggctgt cggtaatggc
    54421 atggtgacgg ttgccgaaca tcaaaatgtt ccttccggca tcgccgccgt agtgattgca
    54481 accgtgcccc tctttaccct gtgcttcagt cgcctgtttg gcattaaaac gcgcaaactg
    54541 gaatgggtgg gtattgccat tgggcttgcc ggaatcatca tgctcaatag cggtggaaat
    54601 ttaagcggca atccgtgggg cgcgattctg attttaatcg gctcgattag ctgggcgttt
    54661 ggctcagttt atggctcgcg cattacctta cctgtaggga tgatggcggg tgcgattgag
    54721 atgctggcgg caggcgtggt gttaatgatc gcatcgatga ttgcgggtga aaaactgacg
    54781 gcgctccctt ccctgtcagg cttccttgcg gtcggctatc tggcgctgtt tggttcgatt
    54841 atcgccatta acgcttatat gtatttaatc cgtaatgtca gtccggctct cgccaccagc
    54901 tacgcctacg ttaacccggt ggtcgcggtc ttgctgggta cgggactggg tggcgaaacg
    54961 ctgtcgaaga ttgaatggct ggcgctcggc gtaattgttt tcgcggtggt actggtcacg
    55021 ttgggaaaat atctcttccc ggccaaaccc gttgtcgcgc cagttattca ggatgcatca
    55081 agcgagtaaa tgaatcccct gcgtgtcgat ctgcgcgctg gcaccttcgc cgcagatcca
    55141 ctcttccaga cgctcggtaa gcgccgcatc cgtcagcttc tcgcgcctac gtaacgcaca
    55201 ctcccagaca atcagtacgc gccagccgag ctcctgcaag cgactgatat cgcggcgatc
    55261 gcgttcaaca tttttaccta tcttctccag ccagaactca gttcgagtcg caggcacttt
    55321 aaacagatag cagtgatgat gatgccagaa gcagccgtgg gtaaatatca cgcagcgata
    55381 ttcatcaacg acaaaatccg ggcgtccggg cagactggcg tcctgaacgc gaaacgcaag
    55441 gccctgcccg gttaacaaac tggcgaggcg cttctcaatc gccgtatcac gcgtggcaat
    55501 cgcgcgcata tttttgctgc gggtggcctt atcgtgaacg tcgaccatgt tgtgcctctt
    55561 gctgacgcaa cgccaccgcc tgtttgattt ttggctcaag cagttttgcc accgcggcaa
    55621 agaccggcac gaccaccgag ttgccgaact ggcgataggc ctgagtgtcc gaaaccggaa
    55681 tgcggaattt cgcttctccc ggcgcttcaa aacccattaa gcgcgcgcat tcacgaggcg
    55741 ttaaccgacg tggacgatgt tgctgattca gcggatcgtc aaagtctttc tcacccgtgg
    55801 ccatatccca accgcgatcg attaaaattt ccgcgccatc tttgtaataa cgagcagaca
    55861 gcgtacgggt gacgctttgc ggattgttcg gataaaccat tccataaccg aagccgttac
    55921 cgcgcgcctg atgttttttc gcatatcgat agaggtactt ccacagcacc ggcgtcagga
    55981 tatatttcgc ctcaaccatc gggtccagca gctgcgccag cgtcactcgc tgtgcaggga
    56041 aacattcgct gatatcacgc agagtaaaat cggctttcag attcagatcg cgacgaaaac
    56101 ccaccagcac gatgcgttca cggtgctgcg gcaggaaatg tttaccatcg atgattttcg
    56161 gatcgtccgg cccgttatct tctgcatcgg caacgtcata gcccagttcg tccagcgtct
    56221 gcatgatgat gcggaacgtt tttcccttat cgtgactctt caggtttttg acgttttcga
    56281 gcacaaacat cgccggacga cgcgcgtcga taatgcgcac cacatcaaag aacagcgtgc
    56341 cctgggtatc gcaggcaaaa ccgtgcgccc gcccgagcga gttctttttc gatacgccag
    56401 ccagcgaaaa tggctgacaa gggaaaccgg ccagtaaaac atcgtgttca ggaatgtgtt
    56461 gacgaatatg ttccgccgcc gcctcatcac tcacgccttc ttgatgactg agggtgatgt
    56521 cgcggatatc ttcattaaaa tgatgcgtcg ccgggtcgca ataatggttg gctttataag
    56581 tgcgtaccgc atgtttgttc cattcgctgg taaacacgca ctgtccacca atcgattcaa
    56641 aaccacgacg gatgccgcca attccggcaa atagatcgat aaagcgaaac gcataatgcg
    56701 gatgatattc cggtggtttg ggtaacaacg tttgcagatg ggcaaactct ttctcactta
    56761 aacggtgcca tgccgagtca ttcgccagcg cacgttttaa aattgccgcg ctccagtgat
    56821 tctcacccac accattaagc tgcgccacca gcattttaac atcataaatt tgcagcagtt
    56881 tctccagcat tgcctgtgcg gcattcccgg tgctgtatga atcggttact gatatatttt
    56941 cctgcataga tttaaccggc catctgagat ggccgacagg ttaacataat tactgccgtt
    57001 tagggccacg cgctcagcca gcgggtgaaa acgcatctat caccttgtga tcgatacctt
    57061 cattctcccc cgccagttcg gcaccgagct tcgccatcaa acagcgccaa gctcaatgcc
    57121 cctgaaacgg caaatacgcg cgccaggcca atcgccccca gggcttccag ccgatccgca
    57181 tcctggacga ttttagcttc cgttgttaag ggggcgattt gcgcgctgaa actgtgagcg
    57241 gcaatggcat gacaaacggc ctcaattttc tccgccggaa attgcacgaa ctcttcacgg
    57301 agcaggcgac gcgtctcttc tgccgccagg attgaagaac gctgccgttg cggatgattt
    57361 ttcgccaggc tcacgatatc gtgaaaataa caggcggtta aaatcaccag catatcgaca
    57421 tcgtcatctg ccgccagttt ctgtgccgta gcccagacac ggcgaaaatg acacaggtca
    57481 tgcgccgcgt cctgatgttg atgatggttt ttcaaccagt tctcgaactg tgcctgccag
    57541 tgttgtaact ccatacgcgc tccgttgcca aagagtcgca accttagcag tttttattca
    57601 tagcgtctgc aacgtgcggg acgaggtcgg gtgtaccatg caatgccgcc taacaaggca
    57661 ccaaccaggg ccataaacag aaatccgacc aatgctccta accacttccc cgcggtagat
    57721 cctaaatcac tttcaaatcc cgacacgggg gcatcgggga tccggctcat cacccagatg
    57781 tatcgcgcca ttgcccagac gaaatagcca caccatgtat agaaagccc
//
LOCUS       DAOFMX010000018        55442 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.018, whole genome shotgun sequence.
ACCESSION   DAOFMX010000018 DAOFMX010000000
VERSION     DAOFMX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.018"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="QBA20_001242"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="QBA20_001242"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDQ6291848.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="QBA20_001243"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="QBA20_001243"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDQ6291849.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="QBA20_001244"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="QBA20_001244"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDQ6291850.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="QBA20_001245"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="QBA20_001245"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDQ6291851.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="QBA20_001246"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="QBA20_001246"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDQ6291852.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="QBA20_001247"
     CDS             7826..9559
                     /locus_tag="QBA20_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDQ6291853.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="QBA20_001248"
     CDS             9708..10559
                     /locus_tag="QBA20_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6291854.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="QBA20_001249"
     CDS             complement(10546..10887)
                     /locus_tag="QBA20_001249"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDQ6291855.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="QBA20_001250"
     CDS             complement(10889..11767)
                     /locus_tag="QBA20_001250"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDQ6291856.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="QBA20_001251"
     CDS             complement(11733..14030)
                     /locus_tag="QBA20_001251"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDQ6291857.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="QBA20_001252"
     CDS             complement(14081..14401)
                     /locus_tag="QBA20_001252"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDQ6291858.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="QBA20_001253"
     CDS             complement(14416..15495)
                     /locus_tag="QBA20_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDQ6291859.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="QBA20_001254"
     CDS             15804..16907
                     /locus_tag="QBA20_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDQ6291860.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="QBA20_001255"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="QBA20_001255"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDQ6291861.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="QBA20_001256"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="QBA20_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="QBA20_001257"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="QBA20_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20983
                     /locus_tag="QBA20_001258"
     CDS             20366..20983
                     /locus_tag="QBA20_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDQ6291862.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="QBA20_001259"
     CDS             complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="QBA20_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDQ6291863.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22009..24189)
                     /gene="katG"
                     /locus_tag="QBA20_001260"
     CDS             complement(22009..24189)
                     /gene="katG"
                     /locus_tag="QBA20_001260"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDQ6291864.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24518..25408)
                     /gene="metF"
                     /locus_tag="QBA20_001261"
     CDS             complement(24518..25408)
                     /gene="metF"
                     /locus_tag="QBA20_001261"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDQ6291865.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25757..28189)
                     /gene="metL"
                     /locus_tag="QBA20_001262"
     CDS             complement(25757..28189)
                     /gene="metL"
                     /locus_tag="QBA20_001262"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDQ6291866.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28192..29352)
                     /gene="metB"
                     /locus_tag="QBA20_001263"
     CDS             complement(28192..29352)
                     /gene="metB"
                     /locus_tag="QBA20_001263"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDQ6291867.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29629..29946
                     /gene="metJ"
                     /locus_tag="QBA20_001264"
     CDS             29629..29946
                     /gene="metJ"
                     /locus_tag="QBA20_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDQ6291868.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30130..30738
                     /locus_tag="QBA20_001265"
     CDS             30130..30738
                     /locus_tag="QBA20_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDQ6291869.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="QBA20_001266"
     CDS             complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="QBA20_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDQ6291870.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31214..33412
                     /gene="priA"
                     /locus_tag="QBA20_001267"
     CDS             31214..33412
                     /gene="priA"
                     /locus_tag="QBA20_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDQ6291871.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33568..34593
                     /gene="cytR"
                     /locus_tag="QBA20_001268"
     CDS             33568..34593
                     /gene="cytR"
                     /locus_tag="QBA20_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDQ6291872.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34685..35644
                     /gene="ftsN"
                     /locus_tag="QBA20_001269"
     CDS             34685..35644
                     /gene="ftsN"
                     /locus_tag="QBA20_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDQ6291873.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35737..36267
                     /gene="hslV"
                     /locus_tag="QBA20_001270"
     CDS             35737..36267
                     /gene="hslV"
                     /locus_tag="QBA20_001270"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDQ6291874.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36277..37608
                     /gene="hslU"
                     /locus_tag="QBA20_001271"
     CDS             36277..37608
                     /gene="hslU"
                     /locus_tag="QBA20_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDQ6291875.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37675..38601
                     /gene="menA"
                     /locus_tag="QBA20_001272"
     CDS             37675..38601
                     /gene="menA"
                     /locus_tag="QBA20_001272"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDQ6291876.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38694..39179
                     /gene="rraA"
                     /locus_tag="QBA20_001273"
     CDS             38694..39179
                     /gene="rraA"
                     /locus_tag="QBA20_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDQ6291877.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="QBA20_001274"
     CDS             complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="QBA20_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDQ6291878.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39935..40794
                     /gene="glpF"
                     /locus_tag="QBA20_001275"
                     /pseudo
     CDS             39935..40794
                     /gene="glpF"
                     /locus_tag="QBA20_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40834..42324
                     /gene="glpK"
                     /locus_tag="QBA20_001276"
     CDS             40834..42324
                     /gene="glpK"
                     /locus_tag="QBA20_001276"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDQ6291879.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42554..43564
                     /gene="glpX"
                     /locus_tag="QBA20_001277"
     CDS             42554..43564
                     /gene="glpX"
                     /locus_tag="QBA20_001277"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDQ6291880.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43661..44407
                     /gene="fpr"
                     /locus_tag="QBA20_001278"
     CDS             43661..44407
                     /gene="fpr"
                     /locus_tag="QBA20_001278"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDQ6291881.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44412..44840)
                     /gene="uspD"
                     /locus_tag="QBA20_001279"
     CDS             complement(44412..44840)
                     /gene="uspD"
                     /locus_tag="QBA20_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDQ6291882.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44867..45166)
                     /locus_tag="QBA20_001280"
     CDS             complement(44867..45166)
                     /locus_tag="QBA20_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDQ6291883.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45378..45818)
                     /locus_tag="QBA20_001281"
                     /pseudo
     CDS             complement(45378..45818)
                     /locus_tag="QBA20_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45919..46518
                     /locus_tag="QBA20_001282"
     CDS             45919..46518
                     /locus_tag="QBA20_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDQ6291884.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46626..47393
                     /gene="tpiA"
                     /locus_tag="QBA20_001283"
     CDS             46626..47393
                     /gene="tpiA"
                     /locus_tag="QBA20_001283"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDQ6291885.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47448..48203)
                     /gene="cdh"
                     /locus_tag="QBA20_001284"
     CDS             complement(47448..48203)
                     /gene="cdh"
                     /locus_tag="QBA20_001284"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDQ6291886.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLSINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48310..49299)
                     /gene="sbp"
                     /locus_tag="QBA20_001285"
     CDS             complement(48310..49299)
                     /gene="sbp"
                     /locus_tag="QBA20_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDQ6291887.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49619..50581)
                     /gene="pfkA"
                     /locus_tag="QBA20_001286"
     CDS             complement(49619..50581)
                     /gene="pfkA"
                     /locus_tag="QBA20_001286"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDQ6291888.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50762..51664)
                     /gene="fieF"
                     /locus_tag="QBA20_001287"
     CDS             complement(50762..51664)
                     /gene="fieF"
                     /locus_tag="QBA20_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDQ6291889.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51812..52312)
                     /gene="cpxP"
                     /locus_tag="QBA20_001288"
     CDS             complement(51812..52312)
                     /gene="cpxP"
                     /locus_tag="QBA20_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDQ6291890.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52462..53160
                     /gene="cpxR"
                     /locus_tag="QBA20_001289"
     CDS             52462..53160
                     /gene="cpxR"
                     /locus_tag="QBA20_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDQ6291891.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53157..54530
                     /gene="cpxA"
                     /locus_tag="QBA20_001290"
     CDS             53157..54530
                     /gene="cpxA"
                     /locus_tag="QBA20_001290"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDQ6291892.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54636..55310)
                     /gene="yiiM"
                     /locus_tag="QBA20_001291"
     CDS             complement(54636..55310)
                     /gene="yiiM"
                     /locus_tag="QBA20_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDQ6291893.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13502 a        15023 c        14168 g        12749 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcatc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccagtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgt caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttatgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccagtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgaaggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgcgctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggttatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatccg gcgtatatgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg
    20581 tgtatgttcc gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact
    20641 ggttcacgaa gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg
    20701 cccggacagc aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca
    20761 caacaaaaca cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc
    20821 ctggcgatta gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga
    20881 cggtacgccg ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt
    20941 tagctggcgg atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga
    21001 agaaaaaatt cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc
    21061 cagtgccagc acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag
    21121 cagccaccag gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat
    21181 actggcaggc aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag
    21241 aatcgcactg taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg
    21301 caccagtaaa gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa
    21361 cgataataaa tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc
    21421 cccccaacta acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag
    21481 ttgccacggc tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg
    21541 cccacgtcgc aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat
    21601 ggtatagctc aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag
    21661 accaaccatc ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg
    21721 gcgcattccg cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca
    21781 gcgtaaggcg gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca
    21841 gctataactc cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt
    21901 tcctgcggca gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta
    21961 aagaaacttc agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc
    22021 gaaacggtcg aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc
    22081 gtgggcatcg ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa
    22141 taccagatcc gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc
    22201 gaacagctct ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac
    22261 gaagaagtca ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc
    22321 gaagttggca cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag
    22381 cgtcagttgc tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc
    22441 gcgatagtta cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt
    22501 ctgatcctga cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc
    22561 ggcgcttgcg gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga
    22621 ggctttacca gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc
    22681 gttcacatcc cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg
    22741 tttgtcgcca ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac
    22801 agacagacca gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt
    22861 gtagatcggc tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc
    22921 gatgtagcga gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa
    22981 ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg
    23041 atcaaaacgc agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc
    23101 gaacggatcc gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg
    23161 actgcgggtc tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg
    23221 ggtcggcgtc tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc
    23281 gctgccgtaa gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc
    23341 aggacctacg tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc
    23401 aatcagcgcc acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc
    23461 tgctgccgca gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag
    23521 gcccatctcg gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag
    23581 ccaggctttt tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc
    23641 ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat
    23701 aaacaggtcg gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg
    23761 acgcgcttta tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg
    23821 accacgaccc gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc
    23881 catacgaata aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc
    23941 tgtcagcagg gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt
    24001 gcggtagtca aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc
    24061 aacacggagt tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc
    24121 gtgaccgccc tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga
    24181 cgtgctcatc aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag
    24241 aagttataat tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa
    24301 agagatgtag atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa
    24361 atgctttata aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg
    24421 ctgacaacct tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt
    24481 tgtgatcact gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca
    24541 gcgtatggca aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt
    24601 tcactccttc acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt
    24661 tgcgggtttc ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac
    24721 gcacgttggt catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc
    24781 ccgggatgat ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc
    24841 tttcgacatc gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt
    24901 tcagattaag caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg
    24961 ccacggatat atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca
    25021 tttctggctt accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac
    25081 cgttattcca gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag
    25141 taagatgcgg tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg
    25201 tacggtcgcg ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc
    25261 tgctaaggcg atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga
    25321 aaaactcgaa cgaaacatta atctgcccct ggacttctgc caggctctga ttcagggcat
    25381 cccgctggct ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg
    25441 ttgtgttgtg cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa
    25501 gggcgtcaac cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact
    25561 tcaggatggt aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac
    25621 taaccatgta cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc
    25681 tatgataatt caatatattt cattagaaac catagacccg aaaacatgag taccgggcat
    25741 tattaaattt ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc
    25801 cggcggtgac gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc
    25861 agcggctttc gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt
    25921 cacgcaccgc ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc
    25981 gcagcaccag ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt
    26041 catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg
    26101 attccacacg tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca
    26161 gcttgcgcat tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct
    26221 gctgccacgc ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca
    26281 gccaggagag cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga
    26341 tcagatcgcg cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc
    26401 agtgacgccc ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg
    26461 ctcccgccag tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact
    26521 gatcagcaag ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg
    26581 cgcgcatcca caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg
    26641 ctaacgcgcg gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca
    26701 cgcctgccag cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga
    26761 acagttccag ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt
    26821 tttctgcgcg gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc
    26881 gcagtactgc caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc
    26941 ctttcagttg ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga
    27001 ccatcgccac cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt
    27061 cgaggatttt cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc
    27121 gatcgttatg cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga
    27181 tctctttatg cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga
    27241 catcatcgtg gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc
    27301 gcgtggaacc ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag
    27361 aaactggctg taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg
    27421 cctcatccag acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac
    27481 tgtatacccc ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga
    27541 tttgtgtcgc ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt
    27601 tacggctgat aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt
    27661 gcagcaacgg gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta
    27721 aaaactcacg ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca
    27781 tcaggcgcgc cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa
    27841 taccactgtc gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc
    27901 tatcggcttc ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg
    27961 tttgttgaac ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca
    28021 actggttagt ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact
    28081 ccgccataat gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa
    28141 atttatgcag ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt
    28201 gtttgcagcc cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc
    28261 ggtggagata cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat
    28321 gcctgcatgt gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc
    28381 cgccagcgta aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc
    28441 cagttcaaaa ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc
    28501 ctgattttcc ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata
    28561 tttcacaatc gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa
    28621 cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca
    28681 ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc
    28741 agagtgaccg ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag
    28801 cggattttgt aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc
    28861 ccttgccaga tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct
    28921 ttctaccagt accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc
    28981 ttgatcaaca aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata
    29041 gctaccgccg tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt
    29101 cgttaccagg tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc
    29161 cagttctgcc agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc
    29221 atgcgcgcgc ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg
    29281 gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg
    29341 tttacgcgtc atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt
    29401 aaacattgtg ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt
    29461 gagcaaatcc caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca
    29521 cgatgtcacg gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa
    29581 attgtggggt ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg
    29641 agcggcgaat atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag
    29701 attacggttt ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt
    29761 caggtgaaca acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat
    29821 gcctttaccg ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa
    29881 atcccggaag cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa
    29941 tactaaagcg gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc
    30001 ctacgtcata ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac
    30061 gccgcatccg gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga
    30121 cgtttaacta tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt
    30181 gcctggcagc cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa
    30241 gcgatccaac tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac
    30301 aaaaagccct acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg
    30361 attgcccatg gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt
    30421 gaacaacagc aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc
    30481 agcttctcat ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag
    30541 aacgcgctgg gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac
    30601 ccgctggtcc aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg
    30661 gtagtttcac cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg
    30721 ccgctgtttt cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct
    30781 tttttcggaa atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc
    30841 aacacggcca ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct
    30901 gcacacgtcg aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca
    30961 agagcagcta gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc
    31021 tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag
    31081 tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg
    31141 tatgcaaact gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag
    31201 tcaggatgat gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt
    31261 tgactatctg ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt
    31321 tggcaaacag caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc
    31381 gctcactgag ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc
    31441 cgtctggcga ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct
    31501 gtttcatgcc ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg
    31561 gtactggttt gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc
    31621 caagcaacaa caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc
    31681 cacgctcgaa tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt
    31741 agcaagtgaa acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg
    31801 gttgcgattg aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac
    31861 tttttctgcc tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag
    31921 tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg
    31981 cctgacaccg caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct
    32041 gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga
    32101 ggcggcgatt gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt
    32161 gattgtcatt gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca
    32221 tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc
    32281 cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg
    32341 cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg
    32401 tcagaaggtg caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca
    32461 ggccaacaat caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg
    32521 ccacgactgc ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca
    32581 ggcgcaacag catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg
    32641 cccttcctgc ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca
    32701 gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag
    32761 ccgcaaaggg gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt
    32821 gatcggcaca caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt
    32881 actggatgtg gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca
    32941 gctttacacc caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct
    33001 gcaaacgcac catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc
    33061 ctttgccgaa caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca
    33121 tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact
    33181 gcgtaatctg atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt
    33241 tccggcactg gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc
    33301 ttcccgcgtg cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc
    33361 ggattcccgc aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta
    33421 cgatgcgagg cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac
    33481 cgttttcacg cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac
    33541 gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc
    33601 atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg
    33661 aatcccgata aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg
    33721 ggttatttac cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg
    33781 gtgattgtcc cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt
    33841 acggcggcaa atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag
    33901 gaaaaaacct ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt
    33961 tcaaggctgc cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg
    34021 gcgaacgaat ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc
    34081 gcatttgatg cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc
    34141 ggtcccgaag agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt
    34201 cgctgcggaa ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc
    34261 ggaagcaaag caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc
    34321 catagcgatg tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc
    34381 ccggaagacc tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg
    34441 ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg
    34501 cttgatcaaa tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt
    34561 atcatccggg gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg
    34621 gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac
    34681 gatagtggca caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa
    34741 gagcacctca cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat
    34801 tgctgccgcc gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa
    34861 agaagagtcc gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc
    34921 agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc
    34981 cacagaacct tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg
    35041 tcagcttctt gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt
    35101 tccgtggaat gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc
    35161 gcagcaatta gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg
    35221 gcagcagcag acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa
    35281 accagcttct actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc
    35341 gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac
    35401 ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga
    35461 acaggcagag acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac
    35521 caacaatggc tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag
    35581 caccctcaat cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg
    35641 ttgaaaccct caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg
    35701 gtttgtactc tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc
    35761 cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg
    35821 aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg
    35881 ggcggtactg cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat
    35941 cagggccatc tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg
    36001 ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc
    36061 ggtaacggtg acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct
    36121 tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt
    36181 gctgaaaagg cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc
    36241 atcgaagaat taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa
    36301 atcgtcagcg aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg
    36361 attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg
    36421 accccgaaaa atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt
    36481 cgtctggcta agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa
    36541 gtgggctacg tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg
    36601 aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa
    36661 gaacgtattc tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag
    36721 cagcaggaac cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt
    36781 gatgacaaag aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct
    36841 cctccgggca tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc
    36901 cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa
    36961 gaagcggcga aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag
    37021 cagcacggga tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc
    37081 ggtccggatg tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc
    37141 accgtttcca ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc
    37201 gcgttccaga ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc
    37261 cgcgttgaac tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat
    37321 gcctctatca ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt
    37381 accgactccg gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa
    37441 aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac
    37501 gacgccagcg atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat
    37561 ctggatgcgt tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc
    37621 attttcatca ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact
    37681 gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc
    37741 ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat
    37801 ccgctggtcg ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc
    37861 gccaatgatt acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta
    37921 cgcggcatgc aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc
    37981 gtcgtgctca tctgtctctc cgggctggca ctggttgcag tggcatgcca tacactggcc
    38041 gattttgtcg gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc
    38101 gtgggcaatc gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt
    38161 ggctggttga gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg
    38221 atccttccgg cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg
    38281 cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa
    38341 gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg
    38401 tttaatctct tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta
    38461 ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg
    38521 ctggaacgta ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc
    38581 ctaagccagt gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca
    38641 gcccacatag cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat
    38701 acgatacttc cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt
    38761 tctccaactt tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg
    38821 aggacaacgg gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg
    38881 tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag
    38941 tacaaaatga atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg
    39001 aagagttgga tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag
    39061 gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc
    39121 atctttatgc cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat
    39181 aagaaaggca ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa
    39241 attacgcgaa agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga
    39301 cgttcctgcc agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg
    39361 cgctgatgct gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct
    39421 tcgatttcca tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt
    39481 ttctcaaaca cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat
    39541 aaaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag
    39601 ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt
    39661 gccacacttt tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa
    39721 tttgttgcgc gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg
    39781 cgataacgct cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg
    39841 ctcgttaacg ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg
    39901 catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg
    39961 ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt
    40021 tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg
    40081 actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc
    40141 cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt
    40201 tatcgtttca caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttagtt
    40261 tacgggcttt actacaattt atttttcgac ttcgagcaga ctcatcacat tgttcgcggc
    40321 agcgttgaaa gtgttgatct ggctggcact ttctctactt accctaatcc tcatatcaat
    40381 tttgtgcagg ctttcgcagt tgagatggtg attaccgcta ttctgatggg gctgatcctg
    40441 gcgttaacgg acgatggcaa cggtgtacca cgcggccctt tggctccctt gctgattggt
    40501 ctactgattg cggtcattag cgcatctatg ggcccattga caggttttgc catgaaccca
    40561 gcgcgtgact tcggtccgaa agtctttgcc tagctggcgg gctggggcaa tgtcgccttt
    40621 accggcggca gagacattcc ttacttcctg gtgccgcttt tcagccctat cgttggcgcg
    40681 attgtaggtg catttgccta ccgcaaactg attggtcgcc atttgccttg cgatatctgt
    40741 gttgtggaag aaaaggaaac cacaactcct tcagaacaaa aagcttcgct gtaatatgac
    40801 tacgggacaa ttaaacatga ctgaaaaaaa tatatcgttg cgctcgacca gggcaccacc
    40861 agctcccgcg cggtcgtaat ggatcacgat gccaatatca ttagcgtgtc gcagcgcgaa
    40921 tttgagcaaa tctacccaaa accaggttgg gtagaacacg acccaatgga aatctgggcc
    40981 acccaaagct ccacgctggt agaagtgctg gcgaaagccg atatcagttc cgatcaaatt
    41041 gcagctatcg gtattacgaa ccagcgtgaa accactattg tctgggaaaa agaaaccggc
    41101 aagcctatct ataacgccat tgtctggcag tgccgtcgta ccgcagaaat ctgcgagcat
    41161 ttaaaacgtg acggtttaga agattatatc cgcagcaata ccggtctggt gattgacccg
    41221 tacttttctg gcaccaaagt gaagtggatc ctcgaccatg tggaaggctc tcgcgagcgt
    41281 gcacgtcgtg gtgaattgct gtttggtacg gttgatacgt ggcttatctg gaaaatgact
    41341 cagggccgtg tccatgtgac cgattacacc aacgcctctc gtaccatgtt gttcaacatc
    41401 cataccctgg actgggacga caaaatgctg gaagtgctgg atattccgcg cgagatgctg
    41461 ccagaagtgc gtcgttcttc cgaagtatac ggtcagacta acattggcgg caaaggcggc
    41521 acgcgtattc caatctccgg gatcgccggt gaccagcagg ccgcgctgtt tggtcagttg
    41581 tgcgtgaaag aagggatggc gaagaacacc tatggcactg gctgctttat gctgatgaac
    41641 actggcgaga aagcggtgaa atcagaaaac ggcctgctga ccaccatcgc ctgcggcccg
    41701 actggcgaag tgaactatgc gttggaaggt gcggtgttta tggcaggcgc atccattcag
    41761 tggctgcgcg atgagatgaa gttgattaac gacgcctacg attccgaata tttcgccacc
    41821 aaagtgcaaa acaccaatgg tgtgtatgtg gttccggcat ttaccgggct gggtgcgccg
    41881 tactgggacc cgtatgcgcg cggggcgatt ttcggtctga ctcgtggggt gaacgctaac
    41941 cacattatac gcgcgacgct ggagtctatt gcttatcaga cgcgtgacgt gctggaagcg
    42001 atgcaggccg actctggtat ccgtctgcac gccctgcgcg tggatggtgg cgcagtagca
    42061 aacaatttcc tgatgcagtt ccagtccgat attctcggca cccgcgttga gcgcccggaa
    42121 gtgcgcgaag tcaccgcatt gggtgcggcc tatctcgcag gcctggcggt tggcttctgg
    42181 cagaacctcg acgagctgca agagaaagcg gtgattgagc gcgagttccg tccaggcatc
    42241 gaaaccactg agcgtaatta ccgttacgca ggctggaaaa aagcggtcaa acgcgcgatg
    42301 gcgtgggaag aacacgacga gtaatgtcag gcacctgaat cgattgcctg atgctgatcg
    42361 cctgatgcga cgcttacgcg tcttatcagg cctacgagcc aatgccattt tgtgtaggcg
    42421 ggataaggcg ttcacgccgc atccggcatc acgcagactc tgaaaccgtg cgggggcaac
    42481 cccgcacaca tcaataatcc cacccttccc ctgtgctaca cttcgcgcca ttccttactg
    42541 cttagagttt gctatgagac gagaacttgc catcgaattt tcccgcgtca ccgaatctgc
    42601 ggcgctggct ggctacaaat ggttaggacg cggcgataaa aacaccgcgg acggcgcggc
    42661 ggtaaacgcc atgcgtatta tgctcaacca ggtcaacatt gacggcacca tcgtcattgg
    42721 tgaaggtgaa atcgacgaag caccgatgct ctacattggt gaaaaagtcg gtactggtcg
    42781 cggcgacgcg gtagatattg ctgttgatcc gattgaaggc acgcgcatga cggcgatggg
    42841 ccaggctaac gcgctggcgg tgctggcagt aggcgataaa ggctgcttcc tcaatgcgcc
    42901 ggatatgtat atggagaagc tgattgtcgg gccgggagcc aaaggcacca ttgatctgaa
    42961 cctgccgctg gcggataacc tgcgcaatgt agcggcggcg ctcggcaaac cgttgagcga
    43021 actgacggta acgattctgg ctaaaccacg ccacgatgcc gttatcgctg aaatgcagca
    43081 actcggcgta cgcgtatttg ctattccgga cggcgacgtt gcggcctcaa ttctcacctg
    43141 tatgccagac agcgaagttg acgtgctgta cggtattggt ggcgcgccgg aaggcgtggt
    43201 ttctgcggcg gtgatccgcg cattagatgg cgacatgaac ggtcgtctgc tggcgcgtca
    43261 tgacgtcaaa ggcgacaacg aagagaatcg tcgcattggc gagcaggagc tggcacgctg
    43321 caaagcgatg ggcatcgaag ccggtaaagt attgcgtctg ggcgatatgg cgcgcagcga
    43381 taacgtcatc ttctctgcca ccggtattac caaaggcgat ctgctggaag gtattagccg
    43441 caaaggcaat atcgcgacta ccgaaacgct gctgatccgc ggcaagtcac gcaccattcg
    43501 ccgcattcag tccatccact atctggatcg caaagacccg gaaatgcagg tgcacattct
    43561 ctgagtgatt tgatcgattg agccttccag tccttcggga ctggaatttt tttgttcgga
    43621 gaacgaagat aaggcaagtc aatcaaaaca ggagaaaaac atggctgatt gggtaacagg
    43681 caaagtcact aaagtgcaga actggaccga cgccctgttt agtctcaccg ttcacgcccc
    43741 cgtgcttccg tttaccgccg ggcaatttac caagcttggc cttgaaatcg acggcgaacg
    43801 cgtccagcgc gcctactcct atgttaactc gcccgataat cccgatctgg agttttacct
    43861 ggtcaccgtc cccgatggca aattaagccc acgattggcg gcactgaaac cgggcgatga
    43921 agtgcaggtg gttagcgaag cagctggctt ctttgttctg gatgaagtac cggactgcga
    43981 aacgctatgg atgctggcaa ccggtacagc gattggccct tatttatcga ttctgcaact
    44041 aggcaaagat ttagatcgct tcaaaaatct ggtcctggtt cacgccgcac gttatgccgc
    44101 cgacttaagc tatttgccac tgatgcaaga actggaaaaa cgctacgaag gcaaactgcg
    44161 cattcagacg gtggtcagtc gggaaacggc agcggggtcg ctcaccggac ggataccggc
    44221 attaattgaa agtggggaac tggaaagcgc gattggcctg ccgatgaata aagaaaccag
    44281 ccatgtgatg ctgtgcggca atccacagat ggtgcgcgat acacaacagt tgctgaaaga
    44341 gacccggcag atgacgaaac atttacgtcg ccgaccgggc catatgacag cggagcatta
    44401 ctggtaagcg gttacttatc gataaacggc acgatgagca aatccgcact catcttattg
    44461 atcatcccgc gatatgccgg catcaaacgg ttgataaatg agtgatgatg accacagaca
    44521 aggaggtcgc actgctcttt ttgcataatt tccagcagtg tttccggcat ttctccgcgt
    44581 tcaatacgca gttttgtctt cggccattga atatttttcg tcagtttata cagcttgtta
    44641 tccgacttat tcttcaacaa ttgaagaata tcttctgttg cagggaagta gatacccgga
    44701 tacaactcgc ttaagccatc atcaatatga attaacgtca ggtgagcgtc attatgtctg
    44761 gcgagctcca gggctttatt caccagtaag gcatcttctt cattcccgga aattgccacg
    44821 ccaatgtgtt tataagccat gtttaactcc ttctaaagca actccatcaa gctaacgaac
    44881 gcagtgataa ctcaaaaata atcatctcgg cctggcatga gaaagtgaag gccgcatcaa
    44941 gctcaacttg cccctccaga tccttaatct tataggccac gctggcccct tcatctgcag
    45001 aggatgcagc ggcggcaagc tctttcagtt tggtcataaa agactctgct tcttcgcggg
    45061 tggcaaaaaa gcgcgaaaac ttactggtac agttgtcgtt atcaatcacc gtaccgatat
    45121 caatcgcaca tcctttagta ctgcatttat ctacgacatc tttcataggg cacctctgta
    45181 tattcgcctc tctgtcgatt acgacttaag aataatgcgc cgttaaggga agaacaaaca
    45241 agtaatgatc ttatggatta ccattttttt tgatattgct taaggtgaag agttcagaag
    45301 gattttccgg gtattgtcat ttgcatcgtg gaaataacaa tcccggaaaa aaatagcagt
    45361 aagagatata ttaaaggtta attacttgat ttattgtcgg ctttatactt cacatcctga
    45421 gtatctttac catatttatt ttcgccttgt gtgccaacaa acgcaccaag atcgataagc
    45481 atcatcacca gaatcaacgt cgggacaaaa cgccccaccg cccattgcca gacacccggt
    45541 aaaatcgctc agttacccgc cagcagcatc cacgccacaa tcatcagaaa tgcccatgcg
    45601 ccggaacgcc cgcgatcatg caagcgctta acagttactg ccgctgttgg ccagagcaag
    45661 cacacaaggc aaaacgccgc ggtctgaata tcgagtagat tcttacccgc cagtgagaaa
    45721 agcaccagca tgcctgcgaa ccacaggcct atccaaatcc agaaatcacg gcgtccaata
    45781 cgccctttaa atgagaataa ccattgctgt atggtcatgt aagttccttg atggttgtct
    45841 tttccaggat tctacccttt tgacaagggc gacagatatc gttttaatcg gagccagtca
    45901 taacaaaagg tactgtcaat gaagccaggg tgtacgctgt tttttctctt atgttctgca
    45961 ttaaccgtta caacaacggc gcatgcacaa acaccagata cggcaacgac cgcgccttat
    46021 ctgctggctg gagcccctac tttcgatctc tccatcagcc agtttcgaga agactttaac
    46081 agccagaatc ccagcctgcc actgaacgaa tttcgtgcca tcgacagcag tcccgacaaa
    46141 gccaatctca ctcgtgctgc aagtaaaatt aacgagaact tgtatgcttc tacagcgctg
    46201 gagcgcggta ccttaaaaat caaaagcatt caaatgacct ggctacccat ccaggggcca
    46261 gagcaaaaag cagcgaaagc gaaagctcag gaatacatgg cagcagtgat ccgcacactc
    46321 accccattaa tgaccaaaac acaaagccag aaaaaactgc agtcgctact aacggcgggg
    46381 aaaaacaaac gttattacac cgagacagaa ggtgcactgc gttatgttgt cgcggacaac
    46441 ggcgaaaagg ggctgacctt cgctgttgaa ccgattaagc tggcgctatc tgaatcgctt
    46501 gaaggtttga ataaatgaca aaaagcaaag cctttgtgcc gatgaatctc tatactgttt
    46561 cacagacctg ctgccctgcg gggcggccat cttcctttat tcgcttataa gcgtggagaa
    46621 ttaaaatgcg acatccttta gtgatgggta actggaaact gaacggcagc cgccacatgg
    46681 ttcacgagct ggtttctaac ctgcgtaaag agctggcagg tgttgctggc tgtgcggttg
    46741 caatcgcacc accggaaatg tacatcgata tggcgaagcg cgaagctgaa ggcagccaca
    46801 tcatgctggg tgcgcaaaac gtggacctga acctgtccgg cgcattcacc ggtgaaactt
    46861 ctgccgctat gctgaaagac atcggcgctc agtacatcat catcggccac tctgaacgtc
    46921 gtacttacca caaagagtct gacgaactga ttgcgaaaaa attcgcggtg ctgaaagagc
    46981 agggtctgac tccggttctg tgcatcggtg aaaccgaagc tgaaaacgaa gcgggaaaaa
    47041 ctgaagaagt ttgcgcacgt cagatcgacg cggtactgaa aactcagggt gctgcggcat
    47101 tcgaaggtgc ggttatcgct tacgaacctg tatgggcaat cggtactggc aaatctgcaa
    47161 ctccggctca ggcacaggct gttcacaaat tcatccgtga ccacatcgct aaagttgacg
    47221 ctaacatcgc tgaacaagtg atcattcagt acggcggctc tgtaaacgcg tctaacgctg
    47281 cagaactgtt tgctcagcca gacatcgacg gcgcgctggt tggcggtgct tctctgaaag
    47341 ctgacgcttt cgcagtgatc gttaaagctg cagaagcggc taaacaggct taagtctgac
    47401 aggtgccgga tttcatatcc ggcacttact ttccttaact cctcgcctta acgcaaaatc
    47461 tcacactcat gatcctggat ttcctcggct gaagcacggt tcagcgtcag tagatttcgt
    47521 tgtgtcgcca gcaacacaaa cgaattatca ctctgccgta ccatcgccag cccgtagctt
    47581 cccatatgtt cccgcgcctc aggcacttct tctgcaagca tcataaatgg gctgcgttgt
    47641 accagttcgc tttccgttac ccgacgcgcc aggtattcat gcccgcgcaa accacctggc
    47701 agtggcaacc agcggctgct gatgttcgcc agatttttat ccagttgttc gcgcacatca
    47761 ggacgaatac aagagatatg aatatgaaaa tggttttgcg tacgcccggt gcgggagttg
    47821 atcgacaaag aaaccgcgcg atcgggaacc ggctggccgt attttttgct cataaaatca
    47881 cgcgcctgcc aggccaacca aaagaagttc ggcgttgaag gatcggtcaa caaaggactt
    47941 tcagtaccgt taatacgata cgttggcatc aacagatatt gcagtggacc attaagatct
    48001 tttaaaacca cgtatccggc attgggtttg acttccgcac atggcgaagg attttgattt
    48061 tgctgctgat tgggcaaaca ttcctcaagg acaatcttac gtaatgtatc cgactcttca
    48121 ccggttaatt tccagtaacc aataccggca gccacaacgg cgataactat catcaccaaa
    48181 aaaagaagac ccgctttttt catctttttt tccctgtacc tcaaagagat gcaagggtaa
    48241 cgcaaaatcg tgacaaataa aaaacccggt cgcgagaaac caccgggttt gataattatc
    48301 ctagggaaat cagcgtttgc tgatctgatc gaacgtaccg ccgttagcaa aatgctcttt
    48361 ttgcgctttc gtccagccgc cgaactcttc atcaatggtg aataacttca gctttggaaa
    48421 cgcattttcg tactttttcg ctacttcagc gtcgcgcgga cggtagtagt ttttcgcggc
    48481 aatttcctga ccttctggcg agtagagata tttcaggtag gcttccgcca cctctttggt
    48541 gccttttttc tcgaccactt tatcgaccac cgacaccgtt ggctctgcga ggatagactc
    48601 actcggcgtg acgatttcga atttatcttt ccccagttca ttcgctgcca gcagagcttc
    48661 gttttcccag gcgatcagta catcgccaat cccgcgctcg acaaaggtgt tagtggagcc
    48721 gcgcgcgcca gaatccagaa cttcgacgtt tttatacagt gcccgaacaa aatcctgtgc
    48781 ttttgcctga tcgttgttgt tgtgatgcag cgcgtagccc caggcagcca ggtagttcca
    48841 gcgtgcgcca ccagagcttt tcggattagg cgtgatcacc gaaacacccg gtttaatcag
    48901 atcgttccag tcatggatct gcttcggatt gcccttacgc accaggaaaa caatggtgga
    48961 agtgtacggt gcggagttat ccggcagacg tttgatccac tctttatcaa tccgcccgcg
    49021 ttccgcaatc gcgtccacgt cataggccag agccagcgtg acaacatcag cttcaatacc
    49081 gttgattacc gacgtcgctt gtttacctga accaccgtgt gactgacgaa tcaccacgtt
    49141 atcaccagtt tgctgtttcc agtgggcgct gaatgccttg ttgtactgtt cgtacaattc
    49201 gcgcgttgga tcatatgaaa cgttaagaag ctgaatatcc tttgccataa cgctggttgc
    49261 cgccagcaaa aatgttaacc ctacgcccca cttgttcatc gcccgactct cttatgttgt
    49321 gttgtgatga gcaaagcgtg ccagaaggtt aaccaaacat taaagaataa aaaaagattg
    49381 gctataactt gcgggtatat gttgagggat taaaaaggcg gaagaaatcc gcctcatatt
    49441 gctgacaaag tgcgctttgt ccatgccgga tgcggcgcga acgccttatc cggcctacaa
    49501 aagtttgcaa attcaataaa ttgcagaatt catgtaggcc tgataagcga agcgcatcag
    49561 gcatttttgc ttctgtcatc ggtttcaggc taaaggaatc tgcctttttc cgaaatcatt
    49621 aatacagttt tttcgcgcag tccagccagt cacctttgaa cggacgcttc atgttttcga
    49681 tagcgtcgat gatgtcgtgg tgaaccagct gttcgttctg gatacctaca caacgaccgc
    49741 cgtaacctgc cagcagcaga tcgatagcgt aagcgcccat acgggaagcc agaatacggt
    49801 cgtaaggcac cggagaacca ccgcgctgga tgtggcccag cacagttgcg cgggtttcac
    49861 gaccggtttc tttctcgatg aaatgcgcca gttcgtcaac atcacacata tgttcggtaa
    49921 tcgccacgat cgcgtgtttt ttacctttcg cgatacccgc tttgatttcg tttaccaggt
    49981 cttcacggct gaattcaact tccggaacta caacgaattc acagccaccg gcaatggccg
    50041 cagccagggt cagatcgcca caataacggc ccatcacttc caccacggaa atacgctggt
    50101 gagaagaaga ggtgtcacgc agacggtcga tcgcttctac aacggtgctc agcgcagtga
    50161 agaaaccgat agtgtagtca gtgcctttga tgtcgttgtc gatagtgccc ggcagaccga
    50221 tgcacgggaa gcccatttcg gtcagacgca ttgcacccat gtaggaaccg tcaccgccga
    50281 taaccaccag cgcgtcgata ccacgttttt tcaggttttc gatagccacg gcgcggatgt
    50341 tctcgtcgcg gaattccggg aaacgcgcag aaccgaggaa cgtaccgcca cggttgatca
    50401 tgtcagacac gctgtaacgg tctagctgta ccatacggtc ttcatacaga cccagatagc
    50461 cgtcataaat acccattact tccagacctt ctgtcagcgc agaacgaaca accccgcgaa
    50521 ttgcggcgtt catgcctggc gcatcaccgc cgcttgtcaa cacacctatt ttcttaatca
    50581 tgactacctc tgaactttgg aatgcaaaat gaaatctgtt gccggaagtc ttcttgcaca
    50641 tcgaagtgat ccaacgaatg tgcaaatagt ataacaatca cttcctgctg aattgattca
    50701 ggtcaggcca aatggcggta ttttatacac aaaatgcggg tctggctctc ttttatactg
    50761 attatgaaag catagaccgt ttaccctccc tgggtacgac ggaacagggg tcctgatgga
    50821 taatcacatc cgatcccgga aaacgccgta aaatagcctg ctctacctga tccgccacca
    50881 tatgtgcctg aaccaaaggc agagagtctt ccatttccaa atgaatctga ataaagcggg
    50941 tcggccctga ctgccgcgtg cgaagatcgt gagcgccgct aacacccggc caggaagtca
    51001 cgatatcaat aatttcttgc cgttcttcat caggcaatgc gcgatccagt aatgactgta
    51061 ccgcctcata tcccatgcgt aacgcgctat ataaaatata gatgccgatt cccaatgcaa
    51121 acagagcatc ggcgcgatgc cagccgtacc aagacaaccc cagcgccagc agaattgcgc
    51181 cgttcatcat aacatcagac tggtaatgta gcatatcagc ccgcaccgcc tggctttgcg
    51241 tccggcgcac cacccaccgc tgaaacgaga caaggataat cgtacaaatt agcgccacaa
    51301 ttgtcacgat aaccccgacg cctggatctg tcatcggtgt tggagatacc agatgttgaa
    51361 tacccgtcaa aaacaggaat agtgccgaac cggagataaa catactttgc gccagcgccg
    51421 cgagggactc agctttaccg tgaccaaacg agtgattatc gtcggcaggt tgcagggaat
    51481 atcgcaccac cagtaaattc gtcaacgacg cgccgatatc caccagcgaa tccaccagcg
    51541 cggcgagaat actcaccgac ccggtatacc accatgcaaa aattttaatc agcaatagca
    51601 acgaagccat cgccgtcgca gcaatcgccg cccgactgac cagccgtcca taagattgat
    51661 tcataaatac tcccgctatc aactgacgct agtataacgg aagcaaatca tctgcaatgc
    51721 attaagcagc aggcaaattg aggataaaaa aaaccccaca tcatgtgggg gaagacaggg
    51781 atggtgtcta tggcaaggaa aacagggttt actactggga acgtgagttg ctactactca
    51841 atagcttcaa cgatgaactt ttttgccatt gcgtcacgtc acgcaactgc tccattcgtt
    51901 gttgatgttt ctcgtttaaa accgcttgct gctccggcgt taacaggcga tacatttggt
    51961 tgcggacttt tgccatctca acctgacgag caatttgctc attcgccatt ttttctgcct
    52021 gtgcgcgcac agcgttttca tcaaaatttt ctgcggtgac aaggcgatgc attgtctcca
    52081 gttcgctaac attaacagga ggctgttcgt gccgggcctg ttgcataaga tctcgcatct
    52141 gctgacgctg atgttcggtt aaacttatgc cgtcgaacat atggctctgc gtactgcgct
    52201 gcgtaagttc ttcacccgga tgccagttat cgcctgaacc gacttcagca gcgtggctta
    52261 atgaactgac tgccagcgtt gaggccatga cggcagcggt aactatgcgc atcatttgct
    52321 cccaaaatct ttctgtcgcg attcaacgat agagagttta cgattcaggc tgcaaacatg
    52381 cgtcaggggg tgtaaaacaa cgtaaagtca tggattagcg acgtctgatg acgtaatttc
    52441 tgcctcggag gtatttaaac aatgaataaa atcctgttag ttgatgatga ccgagagctg
    52501 acttccctat taaaggagct gctcgagatg gaaggcttca acgtgattgt tgcccacgat
    52561 ggggaacagg cgcttgatct tctggacgac agcattgatt tacttttgct tgacgtaatg
    52621 atgccgaaga aaaatggtat cgacacatta aaagcacttc gccagacaca ccagacgcct
    52681 gtcattatgt tgacggcgcg cggcagtgaa cttgatcgcg ttctcggcct tgagctgggc
    52741 gcagatgact atctcccgaa accgtttaat gatcgtgagc tggtggcacg tattcgcgca
    52801 atcctgcgcc gttcgcactg gagcgagcaa cagcaaaaca acgacaacgg ttcaccgaca
    52861 ctggaagttg atgccttagt gctgaatcca ggccgtcagg aagccagctt cgacgggcaa
    52921 acgctggagt taaccggtac tgagtttacc ctgctctatt tgctggcaca gcatctgggt
    52981 caggtggttt cccgtgaaca tttaagccag gaagtgttgg gcaaacgcct gacgcctttc
    53041 gaccgcgcta ttgatatgca catttccaac ctgcgtcgta aactgccgga tcgtaaagat
    53101 ggtcacccgt ggtttaaaac cttgcgtggt cgcggctatc tgatggtttc tgcttcatga
    53161 taggcagctt aaccgcgcgc atcttcgcca tcttctggct gacgctggcg ctggtgttga
    53221 tgttggtttt gatgttaccc aagctcgatt cacgccagat gaccgagctt ctggatagcg
    53281 aacagcgtca ggggctgatg attgagcagc atgtcgaagc ggagctggcg aacgatccgc
    53341 ccaacgattt aatgtggtgg cggcgtctgt tccgggcgat tgataagtgg gcaccgccag
    53401 gacagcgttt gttattggtg accaccgaag gccgcgtgat cggcgctgaa cgcagcgaaa
    53461 tgcagatcat tcgtaacttt attggtcagg ccgataacgc cgatcatccg cagaagaaaa
    53521 agtatggccg cgtggaactg gtcggtccgt tctccgtgcg tgatggcgaa gataattacc
    53581 aactttatct gattcgtccg gccagtagtt ctcaatccga tttcattaac ttactgtttg
    53641 accgcccgct attactgctg attgtcacca tgttggtcag tacgccgctg ctgttgtggt
    53701 tggcctggag tctggcaaaa ccggcgcgta agctgaaaaa cgctgccgat gaagttgccc
    53761 agggaaactt acgccagcac ccggaactgg aagcggggcc acaggaattc cttgccgcag
    53821 gtgccagttt taaccagatg gtcaccgcgc tggagcgcat gatgacctct cagcagcgtc
    53881 tgctttctga tatctctcac gagctgcgca ccccgctgac gcgtctgcaa ctgggtacgg
    53941 cgttactgcg ccgtcgtagc ggtgaaagca aggaactcga gcgtattgaa accgaagcgc
    54001 aacgtctgga cagcatgatc aacgatctgt tggtgatgtc acgtaatcag caaaaaaacg
    54061 cgctggttag cgaaaccatc aaagccaacc agttgtggag tgaagtgctg gataacgcgg
    54121 cgttcgaagc cgagcaaatg ggcaagtcgt tgacagttaa cttcccgcct gggccgtggc
    54181 cgctgtacgg caatccgaac gccctggaaa gtgcgctgga aaacattgtt cgtaatgctc
    54241 tgcgttattc ccatacgaag attgaagtgg gctttgcggt agataaagac ggtatcacca
    54301 ttacggtgga cgacgatggt cctggcgtta gcccggaaga tcgcgaacag attttccgtc
    54361 cgttctatcg gaccgatgaa gcacgcgatc gtgaatctgg cggtacagga ttggggctgg
    54421 cgattgttga aaccgccatt cagcagcatc gtggctgggt gaaggcagaa gacagcccgc
    54481 tgggcggttt acggctggtg atttggttgc cgctgtataa gcggagttaa actccgcatt
    54541 tgtaggcagg ataaggcgtt tacgccgcat ccggcatttg agcaggatgc ctgatgcgac
    54601 gctgatagcg tcttatcagg cctacactcc gggggttatt ttccccacaa ccggcgggaa
    54661 aaatcttcga tcttgccgct cagtcggcgc ttttgcatcg tccgcgtcca gcttttcgat
    54721 aacccggcgg cggagagtaa acggtgatac tgatcgtcat caaacggcat atgccatgcg
    54781 atggcggcag cctcctgcac ggtcacatcg ctgacacggg aaaccaactc cagcggcgcg
    54841 tccgcagaca ctttccctgg cgctatcacg ctatacaacc accccacctt acctgtgttt
    54901 tgcatcaact gcgctatatc gctgatatca aaatggtaat tgagtttata gcaaggcgag
    54961 cgcggctggc tgacctgaat taatgcctct ccccagcgga aaatatcgcc catatgaaca
    55021 ttactttccg tgagaccgtc ggttgagaga ttttcgccaa acgcaggcgc aacaaacaac
    55081 tccgcctgtt ccggaaattc ccgcacccag tagagatagt gctcacgagg ataatgacac
    55141 agcgctctgt ctggcccgcc gtgaaccttt ttctccgcct gctcgtcacc ttccagcccc
    55201 agctccgtca gcatcaactc accatcaacc tggattttgg caattgcgct gggtttgcct
    55261 tcgggataag cctgaatctt gcctgtgtat acatcaaccg gatatcgcat ctgttccctc
    55321 tccacgagaa atttccccat tagaccatat ttattttatg tcagccgtgc tgttttcgcg
    55381 gtaagtatcc aggcaggaac tcaatagcaa accggacgaa cgttagttca tccggctgtg
    55441 gg
//
LOCUS       DAOFMX010000019        53204 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.019, whole genome shotgun sequence.
ACCESSION   DAOFMX010000019 DAOFMX010000000
VERSION     DAOFMX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.019"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(11..322)
                     /locus_tag="QBA20_001292"
     CDS             complement(11..322)
                     /locus_tag="QBA20_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDQ6291894.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="QBA20_001293"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="QBA20_001293"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="QBA20_001294"
     CDS             701..1054
                     /locus_tag="QBA20_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HDQ6291895.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="QBA20_001295"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="QBA20_001295"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDQ6291896.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="QBA20_001296"
     CDS             complement(2810..3280)
                     /locus_tag="QBA20_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDQ6291897.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="QBA20_001297"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="QBA20_001297"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDQ6291898.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="QBA20_001298"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="QBA20_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDQ6291899.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="QBA20_001299"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="QBA20_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDQ6291900.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="QBA20_001300"
     CDS             complement(5283..8432)
                     /locus_tag="QBA20_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDQ6291901.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="QBA20_001301"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="QBA20_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDQ6291902.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="QBA20_001302"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="QBA20_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDQ6291903.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="QBA20_001303"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="QBA20_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDQ6291904.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="QBA20_001304"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="QBA20_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDQ6291905.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="QBA20_001305"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="QBA20_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDQ6291906.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="QBA20_001306"
     CDS             14911..15288
                     /locus_tag="QBA20_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDQ6291907.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="QBA20_001307"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="QBA20_001307"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDQ6291908.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="QBA20_001308"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="QBA20_001308"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDQ6291909.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="QBA20_001308"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="QBA20_001309"
     CDS             18099..18428
                     /locus_tag="QBA20_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDQ6291910.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="QBA20_001310"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="QBA20_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDQ6291911.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="QBA20_001311"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="QBA20_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDQ6291912.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="QBA20_001312"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="QBA20_001312"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDQ6291913.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="QBA20_001313"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="QBA20_001313"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDQ6291914.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="QBA20_001314"
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="QBA20_001314"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDQ6291915.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="QBA20_001315"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="QBA20_001315"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDQ6291916.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="QBA20_001316"
     CDS             complement(25481..27157)
                     /locus_tag="QBA20_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDQ6291917.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="QBA20_001317"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="QBA20_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDQ6291918.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="QBA20_001318"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="QBA20_001318"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDQ6291919.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="QBA20_001319"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="QBA20_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDQ6291920.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="QBA20_001320"
     CDS             complement(31205..31999)
                     /locus_tag="QBA20_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDQ6291921.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="QBA20_001321"
     CDS             32137..32478
                     /locus_tag="QBA20_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDQ6291922.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYF
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="QBA20_001322"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="QBA20_001322"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDQ6291923.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="QBA20_001323"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="QBA20_001323"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDQ6291924.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="QBA20_001324"
     CDS             36394..37686
                     /locus_tag="QBA20_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ6291925.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALGLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38218
                     /gene="cueR"
                     /locus_tag="QBA20_001325"
     CDS             37811..38218
                     /gene="cueR"
                     /locus_tag="QBA20_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDQ6291926.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="QBA20_001326"
     CDS             complement(38219..38677)
                     /locus_tag="QBA20_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDQ6291927.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="QBA20_001327"
     CDS             complement(38674..39591)
                     /locus_tag="QBA20_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDQ6291928.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="QBA20_001328"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="QBA20_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDQ6291929.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="QBA20_001329"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="QBA20_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDQ6291930.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="QBA20_001330"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="QBA20_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDQ6291931.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="QBA20_001331"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="QBA20_001331"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDQ6291932.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="QBA20_001332"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="QBA20_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="QBA20_001333"
     CDS             43550..44236
                     /locus_tag="QBA20_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6291933.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46642
                     /locus_tag="QBA20_001334"
                     /pseudo
     CDS             44233..46642
                     /locus_tag="QBA20_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47354..48448)
                     /gene="mnmH"
                     /locus_tag="QBA20_001335"
     CDS             complement(47354..48448)
                     /gene="mnmH"
                     /locus_tag="QBA20_001335"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDQ6291934.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48517..49443)
                     /gene="allS"
                     /locus_tag="QBA20_001336"
     CDS             complement(48517..49443)
                     /gene="allS"
                     /locus_tag="QBA20_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDQ6291935.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49673..50155
                     /gene="allA"
                     /locus_tag="QBA20_001337"
     CDS             49673..50155
                     /gene="allA"
                     /locus_tag="QBA20_001337"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDQ6291936.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50233..51048
                     /gene="allR"
                     /locus_tag="QBA20_001338"
     CDS             50233..51048
                     /gene="allR"
                     /locus_tag="QBA20_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDQ6291937.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51138..52919
                     /gene="gcl"
                     /locus_tag="QBA20_001339"
     CDS             51138..52919
                     /gene="gcl"
                     /locus_tag="QBA20_001339"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDQ6291938.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52932..>53204
                     /locus_tag="QBA20_001340"
     CDS             52932..>53204
                     /locus_tag="QBA20_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDQ6291939.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12825 a        13691 c        14016 g        12672 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgtggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctggacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgatgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatccct aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt ttgcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tacggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgaccat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct gggtttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagta caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct ctggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtag cgatgaatca ttactgtcgc agtcgctacg atctggtggc gatcctcaaa
    45061 acgctggggg cagggcgagc gcaactgcgt aagctaatcg tcggtcagtg gttgatggtg
    45121 ctgacgcttt cagccgttac cggtggggcc ataggcctgt tgttcgaaaa cgtgttgatg
    45181 gtgctgctca agcccgttct gcctgctgca ctaccgccag ccagcctctg gccgtggctg
    45241 tgggcgcttg gcaccatgac ggtcatctcg ctgctggtgg ggctacgacc ataccgcttg
    45301 ttgctggcaa cgcagccttt acgcgtatta cgtaatgatg tggtcgcgaa cgtctggccg
    45361 ctgaagtttt atctgccgat tgtcagtgtg gtggttgtgc tgctgctcgc cggattaatg
    45421 ggtggcagca tgctgctttg ggcggtgctg gcgggcgcgg tagtactggc tttgctgtgc
    45481 ggtgtgctgg gctggatgct gctgaatgta cttcgccgca tgacgctgaa atcgctgcct
    45541 ctgcgcctgg cggttagccg cctgttacgt cagccgtggt caacgttaag ccagctttcg
    45601 gcattttcgc tctcctttat gctgctggca ctgctgctgg tgctgcgtgg cgatctgctc
    45661 gaccgctggc agcagcagct accgccagaa agcccgaact actttttaat taacatcgcc
    45721 acagaacagg tagcaccgct aaaagcgttc ctcgcggaac atcatatagt cccggaatca
    45781 ttttatccgg tagtgcgggc gcggctgacg gcgattaatg ataagccgac agaaggtaat
    45841 gaagatgagg cgcttaatcg cgaactcaat cttacctggc aaaatacgcg gcccgatcat
    45901 aatccgattg tcgccggtaa ctggccgcca aaagccgatg aagtgtcgat ggaagagggg
    45961 ctggcgaaac gcttaaacgt tgccctcggt gataccgtga cttttatggg cgatacccag
    46021 gagttccgcg ctaaagtgac cagcctgcgc aaagtggact gggaaagtct gcggcctaat
    46081 ttctatttta ttttccctga aggggcatta gacgggcaac cgcagagctg gcttaccagt
    46141 ttccgctggg agaatggcaa cggcatgttg acgcaactca accgccagtt cccgaccatt
    46201 agcctgttag acattggcgc gattttaaaa caggtcggtc aggtgctgga gcaggtaagt
    46261 cgggcgctgg aggtgatggt ggtgctggtc accgcctgcg gtatgttgct gttgctggca
    46321 caggtgcagg tgggaatgcg ccagcgtcat caggagctgg tggtatggcg cacactcggt
    46381 gcggggaaaa aactgctgcg cacgacattg tggtgtgatg ttcgccatgc ttgggtttgt
    46441 ttccggcctg gtggccgcaa ttggtgcgga aacggcactg gcagtgttgc aggcgaaagt
    46501 gtttgatttc ccgtgggagc cagactggcg attgtggatt gttctgccgt gcagcggtgc
    46561 gctgctgctg tcgcttttcg gcggctggct gggtgcgcga ctggttaagg gtaaggcgct
    46621 gttcaggcag tttgtggggt gatgacaggt gttagattgc ctgatgcgct acgcttatca
    46681 ggcctacgaa gagcgtgcaa tatattgatt ttgcaagatt gtgtaggccg gataaggcgt
    46741 ttacgccgca tctggcatga acagtgtgca ttttggcaat aaatatgaaa ccaactgtca
    46801 tgtcggtttt tttaaaggcc gaaggaggta aaccttcggc cttagctacg tttcctcagt
    46861 tattcaataa actgacggat cagttccagc agcgcgatta gcgccttaag aacgatgaga
    46921 actttatcga gaaaagtttt catccgttgc tcccttaagg gagaaagaca atcacagctt
    46981 acccttacgt tgcctataac tatgattttc ttcatgtgct agatgaggac tgtctctctc
    47041 caaattagag caccagaatc agcaccacct gtatcctgac caggacttaa aagtactttt
    47101 ccgcactgcg gtaatccctt gaggcagtat cactactagc gtcgggattg tatcgccaac
    47161 atagctgttt gaatatgtag gcatcctgac ttataattca cttgctagat gaggactgtt
    47221 taatttctgc ccggaaccac caagtcttgg gcggaaaaaa taaaaaaacc gactgtcatg
    47281 tcggtttttt atatctgtat tctggtgcaa ccattttgtt ctcacgacaa tcgccgtttc
    47341 agaataccgc ccatcactgc gccttaaccc ataccgccac ttccgcccac tcaccgcgaa
    47401 agacaacttt ttccgctttt ttctcaagct gatagcgata cattgggtcg taatattctt
    47461 taagtaacgg caccagccag gccagatgac cgtcggtgct gccggtggtg agttgcgttg
    47521 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    47581 taatcgccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    47641 cataagcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcattc aggcgttcaa
    47701 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    47761 gacattccgg caggtgattc gaaccgatca tccggctttc atcttccagc acccacaggc
    47821 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    47881 cctggctaag ttgtggttgt aacgtgcgac caaacgccga accgcgatga cgcgccagcc
    47941 cttccagatc aacaccgttt ggctgttgtt gcactaatag cgttttaccg ctgccggtac
    48001 aaccgccaat cagcactatc ggtttttgtg ccagttcaat ggtcgcctga atcgcggtct
    48061 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct tcatgcagcc
    48121 agcgttgcac aatatgtgaa cgttgaccgc cacgggcgca gcagagaata ccttgcggat
    48181 tttgcaggca cgctgcccgc caggcgtaca tacgctgctg acgaatttca cccgccacca
    48241 gtttatgccc cagcgccagc gctgcgtctg agccttgctg tttatagcag gtgccaacgg
    48301 aggcgcgttc atcgttatcc attaacggca gattgatagc ggcgggcatt gcgccttgct
    48361 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    48421 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    48481 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggg tgccgaaaat
    48541 ttctaaaaat ccgctgattt ccggcctgtg ttgggtaaac aaggtcacaa tatcttctac
    48601 cgctttgcag ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    48661 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    48721 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    48781 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg tgccggcgct
    48841 gtcttcaata ttgaccgccg gaaagcgacg caactgcgct tctgttagcg gctcttcaac
    48901 gttcgccagc ggatgatccg ccgccatgac aaatcgccat tgcaccgatc ctaagggatc
    48961 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    49021 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    49081 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    49141 caggttgttg atgacaatat tcacctggcg ttccacgcca tcattcacct gttgcagctc
    49201 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    49261 agccgctgtc aacgttacgc tacgagtcgt acggaaaaac agcgctactc cggtattctc
    49321 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    49381 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    49441 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    49501 tcagtatttt tacatgataa tcctgttcaa tttgtggact aaatctagtt ttggaaaaac
    49561 attccaactt ttgtattgat gctgttctct taaggtttta gattgcctgt tattgaaacc
    49621 aagctgaccg gtcggcggtg gttgaacgga attatgttac aaggacaaaa agatgaaact
    49681 tcaggtatta ccgttaagtc aggaagcctt tagtgcttat ggcgacgtaa tcgaaacgca
    49741 gcaacgggat tttttccata ttaacaatgg cctggtggag cgttaccacg atttggcgct
    49801 ggttgagatt cttgagcaag accgtacgct tatcagcatt aaccgcgcgc aaccggcgaa
    49861 tctgccgctg accattcacg aactcgaacg tcatccgctg ggcactcagg cctttatccc
    49921 aatgaaaggt gaagtgtttg tggtggtcgt ggcgttaggc gacgacaaac cagacctgtc
    49981 aacgctgcgg gcgtttatca ccaacggtga acagggggtg aattaccatc gtaacgtctg
    50041 gcatcaccca cttttcgcct ggcagcgcgt caccgatttt ctgaccatcg atcgcggcgg
    50101 cagtgacaac tgtgatgttg aaagtattcc tgaacaggaa ctctgttttg cgtgacgcct
    50161 gcaaccgact tgcataagat aaactaattg ttcattgttt atgctcactt gtaggtcgga
    50221 gttaacgtag gtatgacgga agttagacgg cgcggcaggc caggacaggc ggagcctgtg
    50281 gcacagaagg gcgcacaggc gttagagcgg ggaattgcga ttctgcaata tctggaaaaa
    50341 agtgggggaa gttcgtcggt tagcgatatt tctctcaatc tggatttgcc gctctccacg
    50401 acctttcgct tgctgaaggt tttacaagca gcggattttg tctatcagga cagtcaatta
    50461 ggctggtggc atataggatt aggtgtcttt aacgtcggtg cggcgtacat ccataaccgc
    50521 gatgtcctct ccgtcgccgg gccgtttatg cgccgcctga tgttactttc cggcgaaacg
    50581 gtcaatgtcg cgatccgtaa cggcaatgaa gcggtattaa ttggtcagtt agagtgtaaa
    50641 tcgatggtca ggatgtgtgc gccactgggc agtcgtctgc cgctgcatgc ttccggtgcg
    50701 ggcaaagcgc tgctttatcc gctggcggaa gaggagttga tgagcatcat tctgcaaacc
    50761 ggtttgcagc agtttacgcc aactacgctt gtggatatgc ccaccttgct gaaggacctg
    50821 gaacaagcgc gtgaactggg ctataccgta gataaagaag agcatgttgt aggtctgaat
    50881 tgcatagctt cagcaattta cgatgatgtc ggtagagttg ttgccgctat ctccatctcc
    50941 gggccttcat caagactgac agaagatcgt tttgtcagtc agggtgagct ggtcagagac
    51001 accgcccgcg atatcagcac ggcgttggga ctgaaagcac atccatagtg tctgtcgcat
    51061 cccgctctgc ggagcgggtt tttttgacaa aatttgaaag ttggaaaatt tttccaatta
    51121 atagaggtag gaataaaatg gcaaaaatga gagccgttga cgcggcaatg tatgtgctgg
    51181 agaaagaagg tatcaccacc gccttcggcg ttccgggagc tgcaattaat ccgttctact
    51241 cagcgatgcg taagcacggc ggtattcgtc acattctggc gcgtcatgtg gaaggtgttt
    51301 cgcacatggc ggaaggttat acccgcgcaa cggcagggaa tatcggcgta tgtctgggga
    51361 cttccggtcc tgcgggcacg gacatgatca ccgcgctcta ttccgcttct gctgattcca
    51421 ttcctattct gtgcattacc ggccaggcac cgcgcgcccg tctgcataaa gaagattttc
    51481 aggccgtaga tattgaagca attgctaaac cggtcagcaa aatggcggtt acagttcgtg
    51541 aagcggcgct ggtgcctcgc gtgctgcaac aggcatttca cctgatgcgt tctggtcgtc
    51601 cgggtccggt actggtggat ttaccgttcg acgttcaggt tgcggaaatc gagtttgatc
    51661 ctgacatgta cgaaccgctg ccggtctaca aacctgctgc cagccgtatg cagatcgaaa
    51721 aagctgtaga aatgttaatc caggccgaac gtccggtgat tgttgccggg ggcggggtaa
    51781 tcaatgctga cgcagctgca ctgttacaac agtttgctga actgaccagc gttccggtga
    51841 tcccaacgct gatgggctgg ggctgtatcc cggacgatca tgaactgatg gccgggatgg
    51901 tcggtctgca aaccgcgcat cgttacggta acgcaacgtt gctggcgtcc gacatggtgt
    51961 ttggtatcgg taaccgtttt gctaaccgtc ataccggttc ggtagagaaa tacaccgaag
    52021 ggcgcaaaat cgttcatatt gatattgagc cgacgcaaat tggtcgcgtg ctgtgtccgg
    52081 atctcggcat tgtctctgat gctaaagcgg cgctgacact gctggttgaa gtggcgcagg
    52141 agatgcaaaa agcgggtcgt ctgccgtgtc gtaaagaatg ggtcgccgac tgccagcagc
    52201 gtaaacgcac tttgctgcgc aaaacccact tcgacaacgt gccggtgaaa ccgcagcgcg
    52261 tgtatgaaga gatgaacaaa gcctttggtc gcgatgtttg ttatgtcacc actattggtc
    52321 tgtcacaaat cgctgcggca caaatgctgc atgtctttaa agaccgccac tggatcaact
    52381 gtggtcaggc tggtccgtta ggctggacga ttccggcggc gctgggggtt tgtgccgctg
    52441 atccgaaacg caatgtggtg gcgatttctg gcgactttga cttccagttc ctgattgaag
    52501 agttagccgt tggcgcgcag ttcaacattc cgtacatcca tgtgctggtg aataacgcct
    52561 atctcggcct gattcgtcag tcacaacgcg cttttgacat ggactactgc gtgcaactcg
    52621 ctttcgagaa tatcaactcc agtgaagtga atggctacgg tgttgaccac gtaaaagtag
    52681 cggaaggttt aggttgtaaa gctattcggg tcttcaaacc ggaagatatt gcgccagcct
    52741 ttgaacaggc gaaagcctta atggcgcaat atcgggtacc ggtagtcgtg gaagttattc
    52801 tcgagcgtgt gaccaatatt tcgatgggca gcgaactgga taacgtcatg gaatttgaag
    52861 atatcgccga taacgcagcg gatgcaccga ctgaaacctg cttcatgcac tatgaataag
    52921 ggagataaat gatgttacgt ttctctgcta atttatcgat gttatttggt gaatatgatt
    52981 ttctcgcccg ttttgagaaa gctgcgcagt gtggttttcg cggcgttgaa tttatgtttc
    53041 cttatgacta cgacattgaa gaattaaaac aggtactggc gagtaataaa ctcgaacata
    53101 cgctgcacaa tttaccggca ggtgactggg cggcgggcga gcgcggtatt gcctgtattc
    53161 ctggccgtga agaagagttt cgggatggcg tagcagcagc gatt
//
LOCUS       DAOFMX010000020        49828 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.020, whole genome shotgun sequence.
ACCESSION   DAOFMX010000020 DAOFMX010000000
VERSION     DAOFMX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.020"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..1222
                     /locus_tag="QBA20_001341"
     CDS             <1..1222
                     /locus_tag="QBA20_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDQ6291940.1"
                     /translation="ELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENHP
                     VITGHECAEVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMII
                     PEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIA
                     LLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYV
                     NTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNF
                     KVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETV
                     LNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKY
                     LLAQFGVDIGEAAQ"
     gene            1219..2184
                     /gene="yggF"
                     /locus_tag="QBA20_001342"
     CDS             1219..2184
                     /gene="yggF"
                     /locus_tag="QBA20_001342"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDQ6291941.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMHTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2206..2715
                     /gene="fumE"
                     /locus_tag="QBA20_001343"
     CDS             2206..2715
                     /gene="fumE"
                     /locus_tag="QBA20_001343"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDQ6291942.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2712..3425
                     /locus_tag="QBA20_001344"
     CDS             2712..3425
                     /locus_tag="QBA20_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDQ6291943.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3397..4074)
                     /locus_tag="QBA20_001345"
     CDS             complement(3397..4074)
                     /locus_tag="QBA20_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDQ6291944.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4068..4745)
                     /locus_tag="QBA20_001346"
     CDS             complement(4068..4745)
                     /locus_tag="QBA20_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDQ6291945.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4733..5116)
                     /locus_tag="QBA20_001347"
     CDS             complement(4733..5116)
                     /locus_tag="QBA20_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDQ6291946.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5132..5440)
                     /locus_tag="QBA20_001348"
     CDS             complement(5132..5440)
                     /locus_tag="QBA20_001348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291947.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5441..6019)
                     /locus_tag="QBA20_001349"
     CDS             complement(5441..6019)
                     /locus_tag="QBA20_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDQ6291948.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6042..6473)
                     /locus_tag="QBA20_001350"
                     /pseudo
     CDS             complement(6042..6473)
                     /locus_tag="QBA20_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6845..7864
                     /gene="epd"
                     /locus_tag="QBA20_001351"
     CDS             6845..7864
                     /gene="epd"
                     /locus_tag="QBA20_001351"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDQ6291949.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLFLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7914..9077
                     /gene="pgk"
                     /locus_tag="QBA20_001352"
     CDS             7914..9077
                     /gene="pgk"
                     /locus_tag="QBA20_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDQ6291950.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9292..10371
                     /gene="fbaA"
                     /locus_tag="QBA20_001353"
     CDS             9292..10371
                     /gene="fbaA"
                     /locus_tag="QBA20_001353"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDQ6291951.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10562..11422
                     /gene="mscS"
                     /locus_tag="QBA20_001354"
     CDS             10562..11422
                     /gene="mscS"
                     /locus_tag="QBA20_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDQ6291952.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11561..12196
                     /gene="argO"
                     /locus_tag="QBA20_001355"
     CDS             11561..12196
                     /gene="argO"
                     /locus_tag="QBA20_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDQ6291953.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12289..13029
                     /locus_tag="QBA20_001356"
     CDS             12289..13029
                     /locus_tag="QBA20_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDQ6291954.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13196..14146
                     /locus_tag="QBA20_001357"
     CDS             13196..14146
                     /locus_tag="QBA20_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6291955.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14088..15566)
                     /gene="scpC"
                     /locus_tag="QBA20_001358"
     CDS             complement(14088..15566)
                     /gene="scpC"
                     /locus_tag="QBA20_001358"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDQ6291956.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15590..16375)
                     /gene="scpB"
                     /locus_tag="QBA20_001359"
     CDS             complement(15590..16375)
                     /gene="scpB"
                     /locus_tag="QBA20_001359"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDQ6291957.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16386..17381)
                     /gene="meaB"
                     /locus_tag="QBA20_001360"
     CDS             complement(16386..17381)
                     /gene="meaB"
                     /locus_tag="QBA20_001360"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDQ6291958.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17374..19518)
                     /gene="scpA"
                     /locus_tag="QBA20_001361"
     CDS             complement(17374..19518)
                     /gene="scpA"
                     /locus_tag="QBA20_001361"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDQ6291959.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19722..20615)
                     /gene="argP"
                     /locus_tag="QBA20_001362"
     CDS             complement(19722..20615)
                     /gene="argP"
                     /locus_tag="QBA20_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDQ6291960.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20998..21657
                     /gene="rpiA"
                     /locus_tag="QBA20_001363"
     CDS             20998..21657
                     /gene="rpiA"
                     /locus_tag="QBA20_001363"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDQ6291961.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21913..23145
                     /gene="serA"
                     /locus_tag="QBA20_001364"
     CDS             21913..23145
                     /gene="serA"
                     /locus_tag="QBA20_001364"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDQ6291962.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23374..23433
                     /gene="ibsC"
                     /locus_tag="QBA20_001365"
     CDS             23374..23433
                     /gene="ibsC"
                     /locus_tag="QBA20_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDQ6291963.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23766..23825
                     /locus_tag="QBA20_001366"
     CDS             23766..23825
                     /locus_tag="QBA20_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDQ6291964.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23926..24528)
                     /locus_tag="QBA20_001367"
     CDS             complement(23926..24528)
                     /locus_tag="QBA20_001367"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDQ6291965.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24549..24732)
                     /gene="ssrS"
                     /locus_tag="QBA20_001368"
     ncRNA           complement(24549..24732)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="QBA20_001368"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24774..25103)
                     /gene="zapA"
                     /locus_tag="QBA20_001369"
     CDS             complement(24774..25103)
                     /gene="zapA"
                     /locus_tag="QBA20_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDQ6291966.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25271..25849
                     /gene="ygfB"
                     /locus_tag="QBA20_001370"
     CDS             25271..25849
                     /gene="ygfB"
                     /locus_tag="QBA20_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDQ6291967.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25875..27200
                     /gene="pepP"
                     /locus_tag="QBA20_001371"
     CDS             25875..27200
                     /gene="pepP"
                     /locus_tag="QBA20_001371"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDQ6291968.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27197..28375
                     /gene="ubiH"
                     /locus_tag="QBA20_001372"
                     /gene_synonym="visB"
     CDS             27197..28375
                     /gene="ubiH"
                     /locus_tag="QBA20_001372"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDQ6291969.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28398..29600
                     /gene="ubiI"
                     /locus_tag="QBA20_001373"
                     /gene_synonym="visC"
     CDS             28398..29600
                     /gene="ubiI"
                     /locus_tag="QBA20_001373"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDQ6291970.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29674..29757)
                     /locus_tag="QBA20_001374"
     CDS             complement(29674..29757)
                     /locus_tag="QBA20_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291971.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30048..31142
                     /gene="gcvT"
                     /locus_tag="QBA20_001375"
     CDS             30048..31142
                     /gene="gcvT"
                     /locus_tag="QBA20_001375"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDQ6291972.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTWEFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31166..31555
                     /gene="gcvH"
                     /locus_tag="QBA20_001376"
     CDS             31166..31555
                     /gene="gcvH"
                     /locus_tag="QBA20_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDQ6291973.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31674..34547
                     /gene="gcvP"
                     /locus_tag="QBA20_001377"
     CDS             31674..34547
                     /gene="gcvP"
                     /locus_tag="QBA20_001377"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDQ6291974.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34814..35557
                     /locus_tag="QBA20_001378"
     CDS             34814..35557
                     /locus_tag="QBA20_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ6291975.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35614..37047)
                     /gene="bglA"
                     /locus_tag="QBA20_001379"
     CDS             complement(35614..37047)
                     /gene="bglA"
                     /locus_tag="QBA20_001379"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDQ6291976.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37092..37402
                     /locus_tag="QBA20_001380"
                     /pseudo
     CDS             37092..37402
                     /locus_tag="QBA20_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37566..38225
                     /locus_tag="QBA20_001381"
     CDS             37566..38225
                     /locus_tag="QBA20_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDQ6291977.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38421..39401)
                     /gene="ygfZ"
                     /locus_tag="QBA20_001382"
     CDS             complement(38421..39401)
                     /gene="ygfZ"
                     /locus_tag="QBA20_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDQ6291978.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39644..39910
                     /gene="sdhE"
                     /locus_tag="QBA20_001383"
     CDS             39644..39910
                     /gene="sdhE"
                     /locus_tag="QBA20_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDQ6291979.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39891..40298
                     /locus_tag="QBA20_001384"
     CDS             39891..40298
                     /locus_tag="QBA20_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HDQ6291980.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40338..40859)
                     /gene="fldB"
                     /locus_tag="QBA20_001385"
     CDS             complement(40338..40859)
                     /gene="fldB"
                     /locus_tag="QBA20_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDQ6291981.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40971..41867
                     /gene="xerD"
                     /locus_tag="QBA20_001386"
     CDS             40971..41867
                     /gene="xerD"
                     /locus_tag="QBA20_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDQ6291982.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41892..42602
                     /gene="dsbC"
                     /locus_tag="QBA20_001387"
     CDS             41892..42602
                     /gene="dsbC"
                     /locus_tag="QBA20_001387"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDQ6291983.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42608..44341
                     /gene="recJ"
                     /locus_tag="QBA20_001388"
     CDS             42608..44341
                     /gene="recJ"
                     /locus_tag="QBA20_001388"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDQ6291984.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44432..45530
                     /gene="prfB"
                     /locus_tag="QBA20_001389"
     CDS             join(44432..44506,44508..45530)
                     /gene="prfB"
                     /locus_tag="QBA20_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDQ6291985.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45540..47057
                     /gene="lysS"
                     /locus_tag="QBA20_001390"
     CDS             45540..47057
                     /gene="lysS"
                     /locus_tag="QBA20_001390"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDQ6291986.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47100..47648)
                     /gene="idi"
                     /locus_tag="QBA20_001391"
     CDS             complement(47100..47648)
                     /gene="idi"
                     /locus_tag="QBA20_001391"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDQ6291987.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47703..47774
                     /locus_tag="QBA20_001392"
     CDS             47703..47774
                     /locus_tag="QBA20_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291988.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47771..47896)
                     /locus_tag="QBA20_001393"
     CDS             complement(47771..47896)
                     /locus_tag="QBA20_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6291989.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47898..49346)
                     /gene="uacT"
                     /locus_tag="QBA20_001394"
     CDS             complement(47898..49346)
                     /gene="uacT"
                     /locus_tag="QBA20_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDQ6291990.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12051 a        12460 c        13402 g        11915 t
ORIGIN      
        1 tgaactgcca gaaattaccg ataatgaact gttagtgagt gtaatttctg acagcgtctg
       61 tttatcgacc tggaaagcgg cgttactcgg tagtgaacat aaacgcgtac ccgacgattt
      121 agaaaatcat ccggtcatta ccgggcatga atgtgccgag gttattgtcg aagtgggtaa
      181 aaatctcact ggcaaatata aaaaaggcca gcgttttgta ttgcaaccgg cgatggggtt
      241 accaagcgga tattcagcgg gctacagcta cgaatatttt ggcggcaatg ccacttatat
      301 gattattccc gaaatcgcca ttaatttggg ctgcgtatta ccgtatcacg gctcttattt
      361 tgctgcggcg tcgctggcag agcctatgtg ctgcattatt ggtgcttatc atgccaatta
      421 tcacaccacg caatatgttt atgagcatcg catgggcgtc aaacctggcg gcaatattgc
      481 actgctggcg tgtgcgggtc cgatgggcat tggcgctatc gattacgcca ttaacggtgg
      541 catacaaccg tcacgggtgg tggtggtcga tatcgacgac aaacgcctgg cgcaggtgca
      601 gaagctgctg ccggtggatc tggcggccag taaaggcatt gagctggtgt atgtgaatac
      661 caaagggatg agcgatccta tccagatgct gcgggcgctg acaggagatg tcgggttcga
      721 tgacattttt gtttatgcgg cggtgcctgc tgtcgttgag atggctgacg aattactggc
      781 ggaagatggc tgtctgaact tctttgccgg gctgacggat aaaaacttca aagtgccgtt
      841 taatttctac aacgtccatt acaacagcac gcacgtagtc ggtacatccg gcggttcaac
      901 ggacgacatg aaagaggcga ttgcccttag cgccacagga cagttacagc cgtcgtttat
      961 ggtgacccat attggtggcc tggatgcggt gccagaaacc gtgctcaatc tgccggatat
     1021 ccctggcggt aaaaaactca tttataacgg cgtgaccatg ccgctcactg ccattgccga
     1081 ttttgccgaa aaaggcaaaa ccgatccgct gtttaaagag ttggcgcggc tggttgagga
     1141 aacgcacggc atctggaatg aacaggccga gaaatatctg ctggcacaat ttggcgttga
     1201 tatcggggag gccgcgcaat gatgtccctg gcgtggccat tatttcgcgt tacggaacag
     1261 gcagcgttgg ctgcctggcc gcaaaccgga tgtggcgaca aaaataaaat tgatggcctg
     1321 gcggtcaccg cgatgcgcca ggcattaaac gacgtcgctt ttcgtggacg agtggttatc
     1381 ggcgaaggag aaattgacca tgcgccgatg ctgtggattg gcgaagaggt gggcaaaggc
     1441 gatgggccag aagtcgatat cgcggttgat cccattgaag gcacgcggat ggtggcgatg
     1501 gggcagagca atgcgctggc ggtcatggct ttcgctcctc gagatagcct gttacatgcg
     1561 cccgatatgt atatgaaaaa gctggttgtt aatcgactgg ctgccggggc gattgatctg
     1621 tctttgcctc tggcggacaa cttgcgtaac gtggcgcggg cgttaggtaa accgctggac
     1681 aagctgcgca tggttacgct ggataaaccg cgtctgagtg ccgcgattga agaagcaaca
     1741 cagctgggcg tgaaggtttt tgccctgccg gatggcgatg tcgccgccag cgtgctgacc
     1801 tgctggcagg ataatcctta cgatgtgatg cacaccatcg gcggtgcgcc agaaggcgtg
     1861 atttctgcct gtgccgttaa agcgttaggc ggggatatgc aggccgagct gattgacttt
     1921 tgtcaggcta aaggggatta cacggaaaat cggcagattg cagagcagga gcgcaagcgt
     1981 tgtaaggcaa tgggcgttga cgttaaccgt gtgtactcgc tcgatgaact ggtgaggggg
     2041 aacgatatcc tctttagcgc tacgggcgtg acgggcggcg agttggtgaa cggtatccaa
     2101 cagacggcga atggggtgcg gacgcagaca ttactgatcg gcggcgcgga ccaaacgtgt
     2161 aatataatag actccctgca ttgatggtga tctatcggaa gaccaatggc gacgctgaca
     2221 gaagatgatg tgcttgagca actggatgca caggacaatt tattttcatt tatgaaaact
     2281 gcgcattcta ttttgctcca ggggatacgc cagtttctgc cgtcgctgtt tgtcgataac
     2341 gatgaagaga tcgtcgaata tgcagtgaag ccgttactcg cccaaagcgg cccgcttgac
     2401 gatattgatg ttgcgctgcg tttgatttat gcgctgggga aaatggataa atggctgtat
     2461 gccgatatca cgcacttttc ccagtactgg cattacctga acgaacagga tgaaacgccc
     2521 ggatttgccg atgatataac ctgggatttt atcagcaatg tcaacagtat aactcgcaat
     2581 gcgacgctct acgatgcgtt aaaagcgatg aagttcgccg atttcgccgt ctggtcagag
     2641 gcgcgtttta gcggaatggt caaaacggcg ctgacgctgg cagtaacgac caccttaaag
     2701 gaattaacgc cgtgaaaatt gaattaacgg tgaatgggct gaacgtgcaa gcgcagtacc
     2761 atgatgaaga gatagaacgc gtgcataaac tgttgctgcg tatgctggcg gccttacaga
     2821 cggtaaatcc gcagcggcgt acggtggttt ttctttgcgc tccgccgggg acgggtaaat
     2881 ctaccctaac caccttctgg gaatatctcg cgcaacaaga cccggagctg cccgctattc
     2941 aggcgctccc gatggatggt tttcaccatt acaatagctg gctggacgcg catcaattgc
     3001 gccccttcaa aggcgcacca gagacatttg acgttgcgaa actggcggaa aatctgcgcc
     3061 aggtggtgga aggggattgt acgtggccgc agtacgatcg acaaaagcat gatcctgttg
     3121 aagatgcgtt gcacgttacc gcaccactcg tcatcgtcga aggaaactgg ttgttgctgg
     3181 acgacgagaa gtggtgccag cttgcgcaat tttgtgattt ctcgatcttt atcaacgcgc
     3241 cagctgctgc gttgcgggag cgtctggtgg ggcgcaagct ggcgggaggg gtatcgctgg
     3301 cagatgcaga agccttttat gaccgtaccg acgggccgaa tgttcgccgg gtgctggaag
     3361 agagtctgcc tgcaaatttg accttaatga tgaccgctac gggtgaatat cgtctggtgg
     3421 attaacgttc ctgattacac catcttccag ccgcaccaca cgagagaaat gacgccgggt
     3481 aaatgcggcg tcatgactga ttgctaccac gctcgtgcct cgctggcggc acttctccag
     3541 ccagctttca aacacgctca accaattttc gtcaaaatcc cgactcggct cgtccagcag
     3601 taatagtggc ggagagagtg cttccaggca ggcgacggcg accatccgtc gctgcgcact
     3661 atgcagatcc aacgggtgcg agttggcagt gtctgctaac tgacaacatt gcagggcagc
     3721 atgggtacgt tgagtgattt catccgccgg gcatttttgc agtttcaaac caaatgcgat
     3781 ctcgtcagcg acggtgctgt gaaaaagttg gttttccgct tcctgaaaca gtacgccaac
     3841 ctttgccgcg cgctggcggt ttttcaggtt tttcatcgcc tgttgttgca gcataaccgt
     3901 accggctgtg ggcgtaagaa gacctgccat tactcgtaac agcgtcgatt tccctgcgcc
     3961 attgtcaccg gtcagcgcca accattcacc ttgtttgagt tgcagagaaa tgtcgcaaag
     4021 acaatccgtt gccgcgccag gccagcgata agatatctta tttaacgtta acatagtggc
     4081 tggagcgctc cgttttgcaa ttgccacgct tgctggcagt agttcaggaa aggtgtgcga
     4141 tgacgttcaa aaagaataat cagcgaacca cgttcaaacg cccagtgttg taagcgctgc
     4201 aacagcattt cgctggcctg cggcgtcagg cggctaaagg cttcatcaag aattaagagc
     4261 tttggctgca tcgcgatggc gcaggcgatg atcacgcgct gtgtctctcc accggagagt
     4321 gttgccgggt gacgatggcg caacggctgg cactcggtca gagtcagggc ggcatcaatc
     4381 cgcgccataa tctctgcttc agcaagacac agattttctg gcccaaaggc aacttcttct
     4441 tccacgctaa aggtacagcc ggagagctgc aaatagggcg attgctggac gagctggatg
     4501 gtggcggatt gttcattaag cgggagatgc ccaatgggcg taccgaggag cgtccccgtg
     4561 cctgttatct ctcttggcag aaagtccgga taccagcccg ccatcagctg tgccagcgta
     4621 cttttgccac tgccgttgtc accaaagatg gcgaccatcc ccggtttgac atagtgaaag
     4681 tcataacaga acggaggatg cgttgagtgt gtcgggcaat aacggaactg ttctaacgta
     4741 accatatcca cgctccgaat tctgtcagca tcagcaagat catggtgtaa cgcgctacgc
     4801 gttgtagggt gctatcggct ggtgcccaca acgttgttcg tcggttgtgg atccgaaatg
     4861 cccgcatatc cagcgccgca ccacggacag ccagatcgtt aagcgcattg tgtgttagcg
     4921 gaataattaa cgcgggcata gcacgtaaac gctgatacca gccctcatcc agcggcacgc
     4981 cgcgggcacg ctgcgcttca tgaataatcg ctaattgccg ttttaactgc tcaacgacca
     5041 ataacggccc ggcaaacaga taagcgacgc ctggcggcag gcgagaagca aacagagcgc
     5101 gaataaaaca ttgcacgggg acgtactgca ttcacaactg tgaggttgaa acaatcgcca
     5161 gaatcagcaa ccagagcgta atagcatgcg accaccgttc tggcgagcgt ggcgtgccgc
     5221 ttagccattc tggtagccag ccaccgtgta ccagccacag cccggctccc agcgagaaca
     5281 tcagccagac gacatatttt gcccgccgcc gcgtggcctt tagcgcaata aggcagaaaa
     5341 aggtcgccgc gctatagatc ggcaaaatgg tctgcgcggg caggataagc gtggtgcagg
     5401 ccgccaatgc ccacagtgtt aatgaggtaa atggatgcat ttagcgcacg gcagccattg
     5461 ccggaaaatg gcgcgtggtg cgaatcggca gctggcgcag taaaagccag acaatcaccg
     5521 ctgtaagaat tttatcgacc aggttcgcgc ctatcaccgt aatcgccacc gactccacca
     5581 ggttttgccc catcgagtgc atccaggcaa cgaacaaatc ggctccgcta ccggtgacac
     5641 cgccgaacag cgcagtacgc agcggtacag cgacgaccgt taccgcaagc gtaataatca
     5701 cgccgctgac aacgactttt ggcagggtgc gaaaccaacc cgctcgcgcc agccaaccgg
     5761 agaccaggcc gatgaccatc gcgacgggtg caaatgcggc ggcgatcggg tcggtcagca
     5821 atccccagag taaattggtc agtaagccgg tcagcattcc gatcaccgga ccaagcagta
     5881 ccgcgctaat aagtgtgccg atggagtcga gaaatatagg taatttgacc atgctggcca
     5941 gttgcccgcc gatcatattg atggcgatag atataacgat caacaccagt gcctgactgg
     6001 aaaagtggct acgcgccata agaaaacctc tttgaaagta gttatttaac gggggaaata
     6061 tgcaactcgt cgtaaccaga aagcgcgcaa ggttggcggc taaacgccag ttcctcaagg
     6121 ctaccgatca ccagttcaag cgtggtccgc acggtacagc cgggcatcat atgaccgcca
     6181 agcatcgtgc cgtgtggatc ggaaacgcag agatgaagat gctcaccact ctgttccagc
     6241 gtgccgttta gtgagatcac ttcgaatgta ccgttcagga gcgtggtgcc ttcttgcccg
     6301 gcatagcgca aggcgacgtc agtcaggcta ccggtacagc ctgcaatcta ggcggcatga
     6361 agttgatgtt gctgtgcaaa agcgtgtagc tgggaaaaaa cttcctgccc ggggaggagg
     6421 cgcagtgcgt aaaagcgtgc ggaggaagaa tatgagtagg gcaatgtcat cattggagta
     6481 ctccgaaact cggacgtatt gcggataata tcgacgctgc atcagtcata ttctgatata
     6541 agtcagggtt tgtgacggca caacgaaaag attttgctac gcttattcgt gattctgcaa
     6601 agtggaataa attccacagg ggcggggctg aaaacgtgac gtaagtcaga taattgcttt
     6661 ctttgactgg acaatcattc cttttattcc acgtttcgct tatcctagct gaagcgtttc
     6721 agtcgattaa atgttcgaca attaaccaat cagtcgcagt ttgcgacagg taaggtttcc
     6781 ccggacgatt tgctggatta ctctgtctgg caaatttgct cagggaaaac cttgcaggag
     6841 atctatgacc gtacgcgtag cgataaatgg cttcggtcgc atcgggcgta atgtggttcg
     6901 tgctttgtat gaatccggac gccgggcgga aattaccgtg gtggcaatca acgaactagc
     6961 ggatgctgcg ggcatggcgc atttgttgaa atatgacacc agccatggcc gttttgcatg
     7021 ggaagtacga caggaacgcg atcaactttt tgttggtgat gacgccatcc gcgtattgca
     7081 tgaacgttca ctgcaatcgc tcccctggcg tgaacttggc gttgatgtag tcctcgactg
     7141 caccggcgta tatggctccc gcgagcatgg cgaagcgcat attgccgccg gggccaaaaa
     7201 agtgctcttt tcacatcctg gcagtaacga tctcgacgcg accgttgttt acggcgtcaa
     7261 tcaggatcaa cttcgtgcgg aacaccgcat cgtttctaac gcttcctgta ccacgaattg
     7321 cataattccc gtcatcaaat tgttagatga tgcgtacggt attgagtccg gcactgtgac
     7381 cacaattcac tccgccatgc acgatcaaca ggttattgat gcataccatc ctgacctgcg
     7441 tcgcacccgg gcagccagcc agtcgatcat tccggtcgat actaaactgg ccgctggtat
     7501 cacacgattt tttccgcaat ttaacgatcg ctttgaagcg attgcggtac gtgtgccaac
     7561 cataaatgtg acggcaatcg atttaagcgt gacggtgaag aaacctgtaa aagccaatga
     7621 agtcaacctg tttctgcaaa aagcagcaca aggtgcattt catggtatag ttgactatac
     7681 ggaattgccg ttggtctctg tagattttaa ccacgatccg cacagtgcca ttgtcgatgg
     7741 cacccaaacc cgggtcagtg gcgcacacct gatcaaaacg ttggtctggt gcgataacga
     7801 atggggcttt gctaaccgaa tgctcgacac gacgttagct atggctactg ttgctttcag
     7861 gtaagacgca agcagcgtct gcaaaacttt tagaatcaac gagaggattc accatgtctg
     7921 taattaagat gaccgatctg gatcttgctg ggaaacgtgt atttatccgt gcggatctga
     7981 acgtaccagt aaaagacggg aaagtaacca gcgacgcgcg tatccgtgct tctctgccga
     8041 ccatcgaact ggccctgaaa caaggcgcaa aagtgatggt aacttcccac ctgggtcgtc
     8101 ctaccgaagg cgagtacaac gaagaattct ctctgctgcc ggttgttaac tacctgaaag
     8161 acaaactgtc taacccggtt cgtctggtta aagattacct cgacggcgtt gacgttgctg
     8221 aaggtgaact ggttgttctg gaaaacgttc gcttcaacaa aggcgagaag aaagacgacg
     8281 aaaccctgtc caaaaaatac gctgcactgt gtgacgtgtt cgtaatggac gcattcggta
     8341 ctgctcaccg cgcgcaggct tctactcacg gtatcggtaa attcgctgac gttgcgtgcg
     8401 caggcccgct gctggcagct gaactggacg cgctgggtaa agcactgaaa gaacctgctc
     8461 gcccgatggt ggctatcgtt ggtggttcta aagtatctac caaactgacc gttctggact
     8521 ccctgtctaa aatcgctgac cagctgattg ttggtggtgg tatcgctaac acctttatcg
     8581 cggcacaagg ccacgatgtg ggtaaatccc tgtacgaagc tgacctggtt gacgaagcta
     8641 aacgtctgct gaccacctgc aacatcccgg ttccgtctga tgttcgcgta gcaaccgagt
     8701 tctctgaaac tgcaccggct accctgaaat ctgttaacga tgtgaaagct gacgagcaga
     8761 tcctggatat cggtgatgct tccgctcagg aactggctga aatcctgaag aatgcgaaaa
     8821 ccattctgtg gaacggtccg gttggcgtgt tcgaattccc gaacttccgc aaaggtactg
     8881 aaatcgtggc taacgctatc gcagacagcg aagcgttctc catcgctggc ggcggcgaca
     8941 ctctggcagc aatcgacctg ttcggcattg ctgacaaaat ctcctacatc tccactggcg
     9001 gcggcgcatt cctcgaattc gtggaaggta aagtactgcc tgcagtagcg atgctcgaag
     9061 agcgcgctaa gaagtaaaaa atcacagggc agggaaacct gcacttgttt cagcgcgctt
     9121 ttagagcacg cattttttca tactctaaat aattcgagtt gcaggaaggc gacaagagag
     9181 tgaatcccca ggagcttaca taagtaagtg actggggtga gcgaacgcag acgcagcaca
     9241 tgcaacttga agtattatga gtataaggcc cgacgataca ggacaagaga catgtctaag
     9301 atttttgatt tcgtaaaacc tggcgtaatc actggtgatg acgtacagaa agttttccag
     9361 gtagcaaaag aaaacaactt cgcactgcca gcagtaaact gcgtcggtac tgactccatc
     9421 aacgccgtac tggaaactgc tgctaaagtt aaagcgccgg ttatcgttca gttctccaac
     9481 ggtggtgctt cctttatcgc tggtaaaggc gtgaaatctg acgttccgca gggtgctgct
     9541 atcctgggcg cgatctctgg tgcgcatcac gttcaccaga tggctgaaca ttatggtgtt
     9601 ccggttatcc tgcacactga ccactgcgcg aagaaactgc tgccgtggat cgacggtctg
     9661 ctggacgcgg gtgaaaaaca cttcgcagct accggtaagc cgctgttctc ttctcacatg
     9721 atcgacctgt ctgaagaatc tctgcaagag aacatcgaaa tctgctctaa atacctggag
     9781 cgtatgtcca aaatcggcat gactctggaa atcgaactgg gttgcaccgg tggtgaagaa
     9841 gacggcgtgg acaacagcca catggacgct tctgcactgt acacccagcc ggaagacgtt
     9901 gattacgcat acaccgagct gagcaaaatc agcccgcgtt tcaccatcgc tgcgtccttc
     9961 ggtaacgtac acggtgttta caagccgggt aacgtggttc tgactccgac catcctgcgt
    10021 gattctcagg aatatgtttc taagaaacac aacctgccgc acaacagcct gaacttcgta
    10081 ttccacggtg gttccggttc tactgctcag gaaatcaaag actccgtaag ctacggcgta
    10141 gtgaaaatga acatcgatac cgatacccaa tgggcaacct gggaaggcgt tctgaactac
    10201 tacaaagcga acgaagctta tctgcagggt cagctgggta acccgaaagg cgaagatcag
    10261 ccgaacaaga aatactacga tccgcgcgta tggctgcgtg ccggtcagac ttcgatgatc
    10321 gctcgtctgg aaaaagcatt ccaggaactg aacgcgatcg acgttctgta agtcattcct
    10381 ttctgcttat ctcaaggccc gctctgcggg tctttttttc gccaaaagca aggtgattca
    10441 gatgagaaat ctgtgatcta tttggcaaaa ttatgcttta ttgtttaccc ttgtcagact
    10501 gcccgtcata aggcggcgga gtgtatttct ccattttgag tcagttgaaa aggaatattg
    10561 aatggaagat ttgaatgttg tcgatagcat aaacggcgcg ggaagctggc tggtagctaa
    10621 ccaggcgctg ctgctaagtt atgcagtaaa catcgtggcg gcactcgcga tcatcatcgt
    10681 tggtttgatt atcgcgcgga tgatttccaa cgcggtgaat cgcctgatga tctcccgtaa
    10741 aatcgatgcc actgttgctg attttctttc tgcattagtc cgttacggta ttatcgcctt
    10801 tacgctaatc gctgccctgg gacgcgtggg tgtacaaaca gcgtcagtca ttgctgtact
    10861 cggtgccgca ggcttagctg ttggtctggc tttgcagggg tcactttcta acctggccgc
    10921 tggcgtgtta cttgtcatgt tccgcccgtt ccgtgccgga gaatatgttg acctcggcgg
    10981 cgtagccggt actgtgctga gtgtgcagat tttctccacc accatgcgta ctgcagacgg
    11041 taaaattatc gttattccga acggtaaaat tattgccgga aatattatta acttctcccg
    11101 cgagccagtt cgccgtaacg aatttattat tggcgtggcg tatgattccg atatcgatca
    11161 ggttaagcag atcctgacca atattatcca gtctgaagat cgcattttga aagatcgcga
    11221 aatgactgtg cgcctgaacg aacttggtgc atcgtcgatt aatttcgtgg tccgcgtctg
    11281 gagcaacagc ggcgatctgc aaaacgtgta ctgggatgtg ctggagcgta ttaaacgtga
    11341 atttgatgcc gccggtatca gcttcccgta cccgcaaatg gatgtgaact ttaagcgggt
    11401 gaaagaagac aaagctgcgt aatcaacgct gaggccagat aatactctct ggcctctctc
    11461 ttattagtta ttctgattgc caattaatat tatcaatttc cgctaataac aatcccgcga
    11521 tatagtctct gcatcagata cttaattcgg aatatccaac gtgttttctt attactttca
    11581 aggtcttgct cttggggcag ctatgattct gccgctcggc ccacaaaatg cttttgtgat
    11641 gaatcagggc attcgtcgtc agtaccacat tatgattgcc ttactttgcg ctatcagcga
    11701 tttggtcctg atttgcgcgg ggatttttgg tggtagcgcg ttattgatgc agtcgccgtg
    11761 gttgctagcg ctggtcacct ggggcggcgt agtcttcttg ctctggtatg gttttggcgc
    11821 ttttaaaaca gcgatgagta gtaatattga gttagctagt gccgaagtcc tgaagcaagg
    11881 cagatggaaa attatcgcca ccatgttggc agtgacctgg ctgaatccgc atgtttacct
    11941 ggatactttt gttgtactgg gcagccttgg cgggcagctt gacgtcgaac caaaacgttg
    12001 gtttgcgctc gggacaatta gcgcctcttt cctgtggttc tttggtctgg ctattctcgc
    12061 agcctggctg gcaccgcgcc tgcgcacggc aaaatcacag cgcattatca atctggtagt
    12121 gggatgtgtc atgtggttta tagccttgca gctggcgaga gacggtattg ctcatgcaca
    12181 agccttgttc agttaggcgc tatctgatgg aaaaataaaa cagaggcgct aagcttgcct
    12241 ccagaggtcc tgaattttgg gcaagtcaat taacacggag agactaacgt gaagttcaaa
    12301 gttatcgccc tggcggcatt aatgggtatt agcgggatgg cagcgcaggc taacgaattg
    12361 ccggatggac cgcatattgt cacctccggt acggcaagtg tggatgcggt gccagacatt
    12421 gccactcttg cgattgaagt taacgtggcc gcgaaggatg ccgctacagc caagaaacag
    12481 gcagatgagc gcgtcgcaca atacatttcc ttccttgaac tcaatcagat cgcgaaaaaa
    12541 gatatcagct cagcgaattt acgcacccag ccagattatg actatcagga tggtaaaagt
    12601 atcctgaaag gctaccgagc tgtgagaacg gtggaagtca cgctccgtca gttagacaaa
    12661 ctgaattcct tgctggatgg cgcgctgaag gcgggtctta acgaaattcg ttctgtgtcg
    12721 ctgggcgtgg cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc gattgataac
    12781 gcgattcatc aggcgcagga actggcgaac ggctttcatc gtaaactggg gccggtatat
    12841 agcgtgcgct accatgtttc caactatcag cccagcccaa tggtgcggat gatgaaagcc
    12901 gatgccgcgc cggtgtctgc ccaggaaact tacgagcagg ccgctattca gtttgatgat
    12961 caggtcgatg tggtcttcca gttagaacct gtggatcaac aacccgctaa aacacctgca
    13021 gcacaataat acttgtagcc cgacgtattc gtcgggctaa tgcctttacc cgatattgcc
    13081 tcctttttct gtaattttct gtaaaaccat gacgtaatca cctatctttg ctcgcggttt
    13141 catcaaggat aggctgactg aaacggttaa gcggacaagg atgcggggag gggggatgga
    13201 catttttatt tcgaaaaaga tgcgcaattt cattttatta gcgcaaacca ataatattgc
    13261 ccgggcggca gaaaaaatcc atatgacggc ttcgccattt ggtaaaagca ttgccgcgct
    13321 ggaagaacaa attggctata cgctatttac ccgcaaagat aataatatca gcctcaataa
    13381 agccggtcag gaactgtatc aaaaactgtt cccggtttat caacgacttt ctgcgattga
    13441 taatgaaatc cataactcag ggcgtcgttc acgagaaatt gtgattggca tagataatac
    13501 ttatccgaca attattttcg atcagttgat tagtctcgga gataagtatg aaggcgtgac
    13561 cgcccagcca gttgagttca gcgaaaacgg cgttatcgat aatctttttg accgtcagtt
    13621 agattttatt atctccccac agcatgtatc tgcacgagtt caggaactgg aaaacctgac
    13681 tatcagtgaa ttaccaccgc tgcgattggg ctttctcgtt tctcgccgtt atgaagagcg
    13741 tcaagagcaa gagctgcttc aggagctgcc ctggctacaa atgcgctttc aaaaccgggc
    13801 taacttcgaa gcaatgattg atgcgaatat gcgaccttgt gggatcaatc ccaccattat
    13861 ttatcgtccc tacagtttta tggccaaaat cagcgccgtg gagcgcggac attttctgac
    13921 ggtgatcccg cattttgcct ggcgactggt gaatccggca acgctgaaat attttgatgc
    13981 gccgcacagg ccgatgtata tgcaggaata tctttattca atcagaaatc atcgttatac
    14041 cgccacgatc ttcagcatat tgctgaagat cgtgacggga caaaccatta acccagcatc
    14101 gagccggttg caattaaatt acggtgtaag tcgaagacgt ggctaagatc gtggtgaata
    14161 tgtccgccgg gcgcattttc cagatagcga tgcagataat cccgatacat aggatgtgca
    14221 caattatcaa tgatagtgcg ggcgcgttga agcggggaaa gaccgcgcag atcggcgatc
    14281 ccttgttcgg tgatgatcac tttgacgctg tgttcgctgt gatcaacatg gctgcacatt
    14341 ggcacgacgg ttgagatctt gccttcttta gcaatcgacg gagccataaa gatcgacaga
    14401 tacgcgttgc gttcaaaatc accgctgccg ccgatgccgt tcatcagatc gaccccggct
    14461 acgtgcgttg aatttgcatg cccgtaaata tcaaactcca ggccgacgtt cagagcgatg
    14521 acgcccagac gacggatgat ttccgggtta ttggaaatct cctgcggacg caacacaatg
    14581 cggctggcaa agtaatccat attgtcgtaa atcttgcgca gggaatcggc cgagattgtc
    14641 aggctggagg cgctggcccc gctgattttg ccggtttcca gtaaatgcac caccgattcc
    14701 tgtagcactt ccgaatacat catgaacgga ggaatttccg ggttttcccc cagacgtgcc
    14761 attaccgcat tattgatatt gcccacgcca ctttgcagcg gcagaaattc cggcggaata
    14821 cgcccatgcg ccatttcctg caataagaac gtgaccacgt tatcggcaat ctgctggcac
    14881 atgggatttt gcttatccag catattaccg gcgtcgggca agttggtttc cacgacggcg
    14941 acaatctttt tcggatcgat ttgcacatag cgggtaccga cgcgatccat tgcatggaag
    15001 atcgacacgc tattgcgccg cggtggcgcg ccaggaatca caatatctgc cagttctgca
    15061 acgcgcggat cgtgatagtg attaagttca atgatcactt tcttcgctcg cagcagccag
    15121 gtcggcgcat taccgatccc gctggttaac cagactcgac catccggtgc cagtgccgat
    15181 gcttcaatga cggcaacatc aatgtcgccg aagaaaccgt aattgaccat ttgcgccact
    15241 tcgctcaaat gcaggtcaac gaaactcacc gcgccctgat tgatcttttt acgtaaactg
    15301 gacgatgttt gatatggcgc acgccaggaa acagcatcgg cgtcagaaag tacatcgtca
    15361 gcggcggcgc tgattgacgc ccccgtcagc aggcgaattt gatacggctt tttggcctca
    15421 tgctgttcgt tagctctgcg ggcaatcgcg gcgggtaggg ctttcggcga acccgctggg
    15481 gtaaagccgc taaatgccac catgtcgtta tgctggataa tttctgccgc ttcatcggcg
    15541 gtcatccttg tccactgagt ttccattttt actccttcgt tcgcagggat taatgaccaa
    15601 cgaaattagg tttacgtttt tcgaggaaag cgttcatccc ttcctggtaa tcttcgctgt
    15661 cataaaccgc gcggcgcatc ccctgaatac gttcaaattc atcggagttc atggtgtgtg
    15721 cttcgcccag tacacgcagc tcttctttga taacggcaat ggctaacggc gctttctcag
    15781 agatgtggtg cgccatttgt aaggtgaaat cttccagttc ttccacttcc acaacatggt
    15841 tgaggatgcc gacagccagc gcgcgctggg cggtgattgg cgaagcggta aaaatcagct
    15901 ctttgacaat gtggaagccc gcgtcgcggg tcaggttgtg aatgccgacc aggttatacg
    15961 ggacgccgag gtttacaggc gtcattgaga aggttgaggt actggcggcg atgatcagat
    16021 cggaactcat gatcatttca aatgcgccac cccaaacact accttccacc atcgaaatga
    16081 tcggtttcgg gaatttttgg atcatgcggg tgatttgacg caatggatca tcataggaga
    16141 gcggatcgcg accgccagac ggcagttcgt gaatatcgtg acctgcggag aagactttgg
    16201 atccacttgg cgcccgcaaa atgatacagc gaatttccgg ccggttgaga tcgcttaacg
    16261 cctgcataag atcatcaata aagactttac ttaaggcatt aagttttcgt ccatagttaa
    16321 actcaatgac cgccactttg ttgatagtgg caacgttcac atactgataa gacataaaaa
    16381 ttcctttaat caaaatattg cgtctggata aattcactga gctgccgcag gccggtgcgc
    16441 ggtgagagcg tattgttttt gaccgctaaa agcgtctggc ggtaatagcg atcgaaatct
    16501 tcattcgcga acaggtgatt cagtacttct tcttcggtct gcttacgcag ccattccacc
    16561 gattgttgtt gccgcacttg ttgtaaacga ccactggcag ttagcgcggt tttgaagtcg
    16621 atgatggcgt gccagatctc atcgattcca cgtttttcca gtgcgctaca agtcagaccc
    16681 cgtggctgcc attcgtcgta tttacgtcgc agaatatgca gggcactctc gtacatatgc
    16741 cgggcaatgg cgacattggt atggttatcg ccatcgtctt tgttgataac gagcagatca
    16801 gccacttcca tcagcccttt tttaatgccc tgcagatcat cgccgccacc ggcaatttgc
    16861 aacgagataa aacagtccac catgcgggcg acttccgttt ccgactgcgc gacgccaacc
    16921 gtttcgacaa tcactacgtc ataacccgcc gcttcgcata acagcattaa ttcccgtgct
    16981 cgctgactgg caccgcccag atgaccggag gagggtaccg ggcgaataaa cgccgcctcg
    17041 gcacgcgcca ggtcattcat gcgggtttta tccccgagaa tgctaccgcc agtgaccggg
    17101 ctgctgggat cgaccgcaat aacagcaacc tttaatccct cacgaatcaa caacatgcca
    17161 aaggcttcaa gaaaggtact tttccccgcg ccgggtgtgc cggtaacgcc cagtcgcagg
    17221 gcgttaccac agaacggcat aatagcgtca agcagctgtg tacttagcgc ctgatgacgt
    17281 gggtgacggc tttccaccag cgtcatggct tgggcgagcg tggcacgctc accctgacgt
    17341 aagcggcgaa tactttctgc cagcgtggct tcattaatca tgatgctggc ttatcagatt
    17401 cagtacgtcg cgcacactgt cgagcatagg tgtacctgga ccataaatcg ccgccacgcc
    17461 gcgctcttgc aggaaggcgt aatcctgcgg cggaatgacg ccacccgcga ccacgcagat
    17521 atcttcgcgt ccccattttt tcagcgcttc gaccagttcc gggatcagcg ttttatgacc
    17581 ggcagccagt gaggatgcgc ccactacgtg aacgtcgttt tctacggcca ggcgggcgat
    17641 ctcttcaggt gtagagaaca tcgggcttaa atctacgtcg aaaccgagat cggaataggc
    17701 gctggcgatc actttcgcgc cgcgatcgtg tccatcctgg cccatcttag cgatcagaat
    17761 gcgcgggcga cgaccattgt cggcaaggaa ctgctccgtt tgcgcaacaa tggcatcgaa
    17821 ctcggaggcc gatttctcag actgatgata gctttgcgca atcacgccgg taacacactg
    17881 gcttggcacc agataacggt cgaaagcgac ttccagcgca tcggaaattt cacccagggt
    17941 ggcgcgaacg cgagcggcat taacagcggc agccagcagg ttttcgttat gctgtgcggc
    18001 gtgagtcagg gcgttcaacg cggcggttac ggcggcatca tcacgggtgg cgcgaatgcg
    18061 ttccagcgaa gcaatttgct cgttacgcac catcacgttg tcgatctcaa gtacatcggt
    18121 ttcgtcttcg tgatccagtt tgtacttgtt gacaccaacg atgacacgct tgccctggtc
    18181 gatcagcgac tgttcgcgcg ctgaggcctc ttcgatcatt cgttttggca gacctgcttc
    18241 gatcgctttc gccatgccac cggcttcgtc gatctgttgg ataatagctc tggcttgttt
    18301 gacgatttga tcggtcagcg actcaatgta ataggatccg gccagtggat cgacggtgtg
    18361 gcagagttct gattcttcct ggatgatgat ctgggtgttg cgggcaatgc gtgctgagaa
    18421 atcggtaggc aaaccaagcg cttcgtcaaa ggcgttggta tgcagtgact gagtaccgcc
    18481 cagcgtcgca gccagtgctt caatggtggt gcggataacg ttgttatacg gatcctgttc
    18541 agtcaggctc cagcctgagg tctggcagtg ggtacgcagc gccagtgatt tcggatcctg
    18601 tgcgccaaat ccactgaccg cttcgctcca taaataacgt gccgcacgca acatggcgac
    18661 gttcataaac agatccatgc cgataccgaa gaataacgac aggcgaggag cgaagtcatc
    18721 aattttcagt ccggcagaga ttgctgcttt gatgtactca atcccatcgg cgagcgtaaa
    18781 tgctacctgc tgcacgcagt tggcacccgc ttcccccatg tggtaaccgc tgatactgat
    18841 ggtattaaat cgcggcatgt tgccggaaca ccaggcgatg atgtcggcga taatgcgcat
    18901 tgacggtttt ggtgggtaaa tataggtgtt gcggcagagg tactctttga gaatatcgtt
    18961 ttgaatggtg ccggtcagtt tatcaggtgt aacaccttgc tcttctgcgg cgacgatata
    19021 aaacgccagt actggtagca ctgcgccatt catggtcatc gaaaccgaca ttttatccag
    19081 cgggatctgg tcgaacagga ctttcatatc ttccacggtg tcgatagcga cgcccgcttt
    19141 gccgacgtca cccgccacgc gtgggttatc ggagtcgtag ccacggtggg tggcaaggtc
    19201 aaacgcaacg gaaagacctt tttgcccggc ggccaggtta cggcgataaa aagcgttgga
    19261 ctcttttgct gttgaaaaac cagcatactg acggatggtc cacggttggg cggtatacat
    19321 agtggcacgc gggccacgaa cgtagggcgg caaaccagga agggtacctg tcacctccag
    19381 attatcgaga tcggcttcgg tatacagcgg cttgatggcg atcccttccg cggtttgctg
    19441 aaccagcgag tcgacagttt tctcccgacg gctcaattcc ttgttggcaa gctgttgcca
    19501 ctcctgctcg ttagacatta tttgctccgt aaaacgcaat caaaatgagg gtatttgcgc
    19561 ttacggtgag ctttttacgt gggtttgtcg ccagcaaaaa aaatgcattc agacgtcgtt
    19621 ttttgccgcc tgagatttgt ctattttgtg atgtgattca aggaatacaa aaagaagtga
    19681 ggacaacgcc tgatatgtgc tatcaggcat tatttgatgg attaatcctg acgaaggact
    19741 ttgtgaccat aatcgagtaa cgcatcagtg actttacgca tcatgcggct ttcaggagca
    19801 aagcggtgcc agtagagcat ccgtcgttga aatagcccag gcgttaagtc aatcagttca
    19861 ccgctggcca gctctttctc gatttgcagg tgcgggatca tacagcaggt ggtgccctgg
    19921 cgagcaagtt gtacgaacgc ttctgaagaa ttaacgatat ggcagggtac gctgccagga
    19981 ggaagatcga agttttgctg caaaaaggcc tggtgcatat cgtcaagatg gtcaaacgcg
    20041 accactggcg ctttcaataa tgccgaacgc gttacgccgt taggaaaata tttttcggca
    20101 aacggttttg agctgacgaa cagatagtcg agcgcaccga gtttatcgac aagacaactc
    20161 ggcagcgcct gatgttgaat actcaccgcg ccgaccactt cgccgcggcg cagacgttcc
    20221 tgagtacggg tttcatcttc tacctgcaag ttgaggcgga taggcgaatc agccaacaca
    20281 ggagccagtg ccggaagcaa ccacgtcgcc agactgtcgg cgttgaccgc cagtgaaagc
    20341 agcagcggag tcgaaccggt ttgttcatcg cccagccact cttcttccag caactccacc
    20401 tggcgcagca gtgccagcag tttttgccct tgttccgtcg ggcgcggcgg tacggtacgc
    20461 accaacagcg gctgcccgaa catattttcc agttgcttaa tgcgctgtga gacggctgat
    20521 tgtgtaatgc acagcttttg tgccgcgcgc tcaaatcctc gttcacgtat caccgcatcc
    20581 agtgcctgta atgttctgta gtccgggcgt ttcattgctg ttgtctgctc ctggtaaatg
    20641 ctttttcctg cactatgaca caattttgtg tcaggttgca tataccgaag ccgtcggcgg
    20701 gtaacatttg tatcaatggt taaacgcgca gcttaaaaga tcagggattt gcgctattgc
    20761 atttcgctta gcgacttcgc gccctggtta tgttgaatgg cgtggcgtta ttgcctctgt
    20821 tcgcaccgtg gagcggcata cccagaccgt tgtatgcttt atttgcgggg cgaaagggga
    20881 tgcccgccat tgcgcgatat tttatggatg agttaaccac ccggcttgcc aacggggtct
    20941 gaatcgcttt ttttgtatat aatgcgtgtg aaatttcata ccacaggcga aacgatcatg
    21001 acgcaggatg aattgaaaaa agcagtagga tgggcggcac ttcagtatgt tcagcccggt
    21061 accattgttg gtgtaggtac aggttccacc gccgcacact ttattgacgc gctcggcacg
    21121 atgaaaggcc agattgaagg ggccgtttcc agttcagatg cttccactga aaaactgaaa
    21181 agcctcggca ttcacgtttt tgatctcaac gaagtcgaca gccttggcat ctacgttgat
    21241 ggcgcagatg aaatcaacgg ccacatgcaa atgatcaaag gcggcggcgc ggctctgacc
    21301 cgtgaaaaaa tcattgcttc ggttgcggaa aaatttatct gtattgccga cgcctccaag
    21361 caggttgata ttctgggtaa attcccgctg ccagtagaag ttatcccgat ggcacgtagt
    21421 gcagtggcgc gccagctggt gaaactgggc ggtcgtccgg aataccgtca gggcgtggtg
    21481 accgataatg gcaacgtgat cctcgacgtc cacggcatgg aaatccttga cccgatagcg
    21541 atggaaaacg ccataaatgc gattcctggc gtggtgactg ttggcttgtt tgctaaccgt
    21601 ggcgcggacg ttgcgctgat tggcacacct gacggtgtca aaaccattgt gaaatgatct
    21661 gacgggggaa cctcccccgt taaaaaaatt ctcttcatta aatttggtga catgtgtcac
    21721 gcttttacca ggcaattgtc gattgctcta aataaatcct ctaaaccagc atattcatcc
    21781 aagaattacc tttgcgtgat atttcctcaa catcgcgacg caaacgttca tattgccgca
    21841 atattatttt ttgatatgtt gaaaggcgga tgcaaatccg cacacaacat ttcaaaagac
    21901 aggattgggt aaatggcaaa ggtatcgctg gagaaagaca agattaagtt tctgctggta
    21961 gaaggcgtgc accaaaaggc gctggaaagc cttcgtgcag ctggttacac caacatcgaa
    22021 tttcacaaag gcgcgctgga tgatgaacaa ttaaaagaat ccatccgcga tgcccacttc
    22081 atcggcctgc gatcccgtac ccatctgact gaagacgtga tcaacgccgc agaaaaactg
    22141 gtcgctattg gctgtttctg tatcggaaca aaccaggttg atctggatgc ggcggcaaag
    22201 cgcgggatcc cggtatttaa cgcaccgttc tcaaatacgc gctctgttgc ggagctggtg
    22261 attggcgaac tgctgctgct attgcgcggc gtgccggaag ccaatgctaa agcgcaccgt
    22321 ggcgtgtgga acaaactggc ggcgggttct tttgaagcgc gcggcaaaaa gctgggtatc
    22381 atcggctacg gtcatattgg tacgcaattg ggcattctgg ctgaatcgct gggaatgtat
    22441 gtttactttt atgatattga aaacaaactg ccgctgggca acgccactca ggtacagcat
    22501 ctttctgacc tgctgaatat gagcgatgtg gtgagtctgc atgtaccaga gaatccgtcc
    22561 accaaaaata tgatgggcgc gaaagagatt tcgctaatga agcccggctc gctgctgatt
    22621 aatgcctcgc gcggtactgt ggtggatatt ccggcgctgt gtgatgcgct ggcgagcaaa
    22681 catctggcgg gggcggcaat cgacgtattc ccgacggaac cggcgaccaa tagcgatcca
    22741 tttacctctc cactgtgtga attcgacaac gtccttctga cgccacacat tggcggttcg
    22801 actcaggaag cgcaggagaa tatcggcttg gaagttgcgg gtaaattgat caagtattct
    22861 gacaatggct caacgctctc tgcggtgaac ttcccggaag tctcgctgcc actgcatggt
    22921 ggtcgtcgtc tgatgcacat ccacgaaaac cgtccgggcg tgctaactgc gctgaacaaa
    22981 atcttcgccg agcagggcgt caacatcgcc gcgcaatatc tacaaacttc cgcccagatg
    23041 ggttatgtag ttattgatat tgaagctgac gaagacgttg ccgaaaaagc gctgcaggca
    23101 atgaaagcta ttccgggtac cattcgcgcc cgtctgctgt actaattccc cttctctgaa
    23161 aattaacggg caggtcactg acttgcccgt ttttttatcc cttctccaca ccgttgccgc
    23221 gttgcgcatt ctactttgcg agtctgctcc agaaaataaa tcaaagtcag atcaatgcgt
    23281 tgtgtttatt taatggggat ttacgatggc gggacagcat ggggctgtaa cggtaaagcc
    23341 ctcaccgaag cgagggctta aaggagaagg gttatgatgc gacttgtcat catactgatt
    23401 gtactgttac tgataagttt cggagcttat taacagtcag tctcaaggga ggagtcatcc
    23461 tcccttaccc ttactcacta aattaggtca aagaatcaac gatgtcaatc agggcgatgc
    23521 ggggtgcatc gccctgaacc gaactttttg atagccactt catcgcccac gtttactgcc
    23581 gtaatagatc ctttttccca aaaattatct ctgttttgca tggggttttt cgagcacgct
    23641 cgaagaaaag atttaaaggt agatcaatgc gttgtggtta tttaaagaga gcttacgata
    23701 gctgggcagc atggggctgt aacggtaaag ccctcaccga agcgagggct tgaaggagaa
    23761 gggttatgat gcgacttgcc atcatactga ttgtactgtt actcataagt ttcccagctt
    23821 attaacagtc agtcacaggg gaggagcaat cctcccttac ccttactcac taaattaggt
    23881 taaagaatca acgatgtcaa tcagggcgat gcaggtgtat cgcccttacc actcccagac
    23941 tttcgacggt gtaaccaccg caggaagagg gatatcccac tcttcaacgg ggagtttttc
    24001 caccaactga caatcatgcg cataacccac cggttgcgtt ttatagtgct gccagttttg
    24061 taaggtccga tcataaaaac caccgcccat tcccaggcgc tgaccgtact catcaaaggc
    24121 gaccagcggt gtgatcagca cgtctaatcg ggaaaggggt agcacgtcac gcacatccag
    24181 ttttggctca tggatcttca acctgttcat caccagttcg ctttgcggat ggtaattcag
    24241 gaacagcaaa ttaccggcac taaagggatg caaaactgga agatatacgc gcttaccggc
    24301 gcgccagagt tgttctatta gcggctgggt gtcgagttca ccatcaaaag agaggaatac
    24361 agcgaccgta tgcgccatca ccaccggggg ataagtcatc atccgggtag cggcttgttg
    24421 acccatttcc agctgttgtt ccggcgttaa cgcacgacga cgttgccgaa tcattttgcg
    24481 gatttcttgt cgggataatg tcagtgggag ctctggtagt tgcgtcatgg ctggtgccag
    24541 ataagaaggg aatctccgag atgccgccgc aggctgtaac ccttgaaccc ttggttcaag
    24601 gtgaatgtgt cgtcgcagtt ttaaggcttc tcggacgaac cgagcatgct caccaaccgc
    24661 ggagcgccac attcttgtgg tatgaaatat cggctcaggg gactggcccg cttgcgaaca
    24721 tctcagagaa attttgtctt cacggttact ctaccacagt aaaccgaaaa gtgtcattca
    24781 aagttttggt tagttttttc ggtgatgcga ccttgttcaa gtaacgcttg ttctatggtc
    24841 tgctgcagca tccgaatacg ctgttccata cttgccgcgt agtcacgagt ctttgctttt
    24901 tcttgcgcta actcatagct gatattcaat gcggcaatga agaccaactg ttcagtattt
    24961 gtgactctag tgcgttcttt cagatcttgc aaccgttggt tcagatcgtc cgctgcctga
    25021 ttcaacgcat ccctttggtc aggcgggcag ttcacacgca gtgaacggcc aaaaatttgg
    25081 atatcgacgg gttgtgcaga catgccacct tcctgctgat tgactgcgct gccttcgtct
    25141 ttagacccat ggtctgcgaa ggggcgacac tatagctacc ctgatgagaa gagacaagcc
    25201 cttttctggt ccaccagggc ccaaagtggt agcatatcat gaatattcct ccctttgacg
    25261 acgaatgctt atgtctatac agaacgaaat gcctggttac aacgaaatga accagtatct
    25321 gaaccaacaa gggacgggtc tgacccctgc cgagatgcat ggtttaatca gtgggatgat
    25381 atgtggcggt aacgatgaca gctcatggct gccgctactt cacgacctga cgaacgaagg
    25441 catggctttc ggtcatgagc tggcacaggc actgcgtaaa atgcactctg ccaccagcga
    25501 tgccctgcag gatgacggct tcctttttca gctttatctg cctgatggcg atgatgtcag
    25561 cgttttcgat cgggctgatg cactggcagg ttgggtcaat cacttcctgc ttggtcttgg
    25621 cgttacgcaa ccgaagctgg acaaagtgac cggcgaaacc ggtgaagcca tcgacgatct
    25681 gcgtaacatc gcgcagctgg gctacgacga agacgaagat caggaagagc ttgaaatgtc
    25741 gcttgaagag atcatcgaat acgttcgtgt tgccgcgctg ttatgccacg acacctttac
    25801 tcatccgcaa ccgaccgcgc cagaagtaca aaaaccgact ctacactaaa aacaaaaaac
    25861 gtaaggagag tgttatgagt gagatatccc ggcaagagtt tcagcgtcgc cgtcaggccc
    25921 tggtggagca aatgcaaccc ggcagcgccg cgctgatttt tgctgcacca gaagtaacac
    25981 gtagcgccga cagcgaatac ccctatcgtc agaacagtga cttctggtac ttcaccggct
    26041 ttaacgaacc ggaagcggtg ctggtgctga ttaaaagcga tgacactcat aaccacagcg
    26101 ttctgtttaa ccgcgttcgc gacctgacgg cggagatctg gtttggccgt cgcttaggcc
    26161 aggatgccgc gccagagaaa ctgggcgttg accgcgcact ggcattcagc gaaatcaatc
    26221 agcaacttta tcaactactt aacggcctgg atgtggttta ccatgcccag ggcgaatatg
    26281 catatgctga tgaaatcgtg aacagtgcgc tggaaaaact gcgtaaaggt tcgcggcaaa
    26341 atctcaccgc accggcaacg atgatcgact ggcgtcctgt tgttcatgaa atgcgcctgt
    26401 tcaaatcgcc agaagagatt gccgtactcc gccgcgcggg agaaatcacc gccatggcac
    26461 atacacgggc gatggaaaaa tgccgtccgg gaatgttcga gtaccatctg gaaggcgaaa
    26521 ttcaccacga atttaaccgc cacggtgcgc gctatccttc ctataacacc attgtaggca
    26581 gcggtgaaaa cggctgcatt ctgcactaca ccgaaaacga gagtgaactg cgcgacggtg
    26641 acctggtgtt gattgacgcg ggttgtgaat acaaaggtta cgcgggcgat attacccgca
    26701 ccttcccggt caacggcaaa ttcacccagg cccagcgtga aatctacgac attgtgctgg
    26761 agtctctcga aaccagcctg cgcctgtatc gtccgggaac ttccattctg gaagtcactg
    26821 gtgaagtggt gcgcatcatg gttagcggcc tggtaaaact cggcatcctg aaaggtaatg
    26881 ttgatgaact gatcgctcag aacgcccatc gtcctttctt tatgcatggc cttagccact
    26941 ggttaggact ggatgtccat gacgttggcg tttatggtca ggatcgctcg cgcattctgg
    27001 aaccggggat ggtactgacc gtagagcctg gtctgtatat cgcgccggat gcggatgtgc
    27061 cagaacaata tcgcggtatc ggcattcgca ttgaagacga cattgtgatt accgaaaccg
    27121 gcaacgaaaa cctcaccgcc agcgtggtga aaaagccgga agaaatcgaa gcgttgatgg
    27181 ctgctgcgag aaagcaatga gcgtaatcat cgtcggtggc ggcatggcgg gcgcgacgct
    27241 ggcgctggct atttcccggt taagtcacgg ggcgctgccg gtacatttga ttgaagcgac
    27301 tgcgccagag tcacatgctc atccgggctt tgatggacga gcgatagcgc tggcggcggg
    27361 tacctgtcag caactggcgc gcatcggcgt ctggcaatct ctggctgatt gcgcaaccgc
    27421 catcactacc gtgcatgtca gcgatcgtgg tcatgctgga ttcgtcaccc tcgccgcaga
    27481 agattaccaa ctggcggcgc tggggcaggt tgtcgaattg cataatgtcg ggcaacggct
    27541 gtttgcattg ctgcgaaaag cacctggcgt aatgctgcat tgccccgatc gcgtagctaa
    27601 cgttgcccgt actcagagtc atgttgaagt gacgctggag agcggcgaga tgctgacggg
    27661 ccgcgtgctg atagccgctg atggcaccca ttcagcgtta gccaccgcgt gcggcgttga
    27721 ttggcagcag gagccttacg aacaactggc agtgattgcc aacgttgcta cttctgttgc
    27781 gcacgaaggc cgcgcttttg aacgcttcac gcaacatggc ccgctggcga tgttgccgat
    27841 gtctgacgga cgctgttcgc tggtctggtg tcatccactg gaacggcgcg aagaggtgct
    27901 gtcgtggagc gacgagaagt tttgccgtga actccagtct gcctttggct ggcgactggg
    27961 gcaaattacc cacgccggta aacgcagtgc ttatccgctg gcattaaccc gcgccgccag
    28021 accgattacc catcgcactg tgctggtggg caatgcggcg caaactctgc acccgattgc
    28081 cgggcaaggg tttaacctcg gtatgcgaga tgtcatgagc cttgcggaaa ccctgactca
    28141 ggcgcatgag cgcggagaag acatcggtga ttacggcata ttgtgccgct atcagcagcg
    28201 tcgacagagc gatcgcgaag caaccatcgg cgtcacggac agcctggtac atctttttgc
    28261 caaccgttgg gcaccgctgg ttgtcgggcg caacatcgga ctgatgacga tggaattatt
    28321 caccccggca cgcgatgtgc tggcgcagcg caccctcggt tgggtggcgc gttgaaggcg
    28381 gttttaagga ataaacaatg caaagtgttg atgtagccat tgttggcggc ggcatggtgg
    28441 ggctggcggt tgcctgcggc ttacagggga gcggcttacg cgttgccgta ctggagcagc
    28501 gcgtaccgga acctctggcg gtagatgcac taccacaact gcgcgtttcg gctatcaatg
    28561 ccgccagcga aaaattactc acccgtcttg gcgtctggca ggacattctc gctcgtaggg
    28621 ccagttgtta tcacggcatg gaagtgtggg acaaagacag ttttggtcac atctcgtttg
    28681 acgatcaaag catgggctat agccatcttg ggcacatcgt tgaaaactca gtgattcact
    28741 acgcgctgtg gaacaaagcg cagcagtcgt cagatattac tctgttggcc cccacagaat
    28801 tacagcaggt cgcctgggga gaaaatgaaa ccttcctgac gctgaaagat ggcagcatgt
    28861 taacggcgcg tctggtgatt ggcgcggacg gtgctaattc ctggttgcgc aacaaagcag
    28921 atattccgct gactttctgg gattatcagc atcacgcgct ggtagcgacc attcgcacgg
    28981 aagaaccgca tgatgcggtg gcgcggcagg ttttccatgg cgaaggcatt ctggcctttt
    29041 taccgcttag cgatccgcat ctttgctcga ttgtctggtc actgtcgcca gaggaagcgc
    29101 agcggatgca gcaggcaagt gaagacgaat ttaatcgcgc gttaaatatc gcttttgata
    29161 atcgccttgg cttatgcaag gttgagagcg cgcgtcaggt gttcccgctg acggggcgtt
    29221 atgcgcgcca gtttgccgca caccgtctgg cgctggtggg cgacgccgcg cataccattc
    29281 acccactggc ggggcagggc gttaatctcg gatttatgga cgccgcagag ctgattgctg
    29341 aactgaaacg gttgcatcgt cagggtaaag acatcgggca gtacatttac ctgcgccgct
    29401 atgaacgtag ccgcaagcac agcgcggcgc tgatgctggc tggtatgcag ggttttcgcg
    29461 atctcttttc cggcgctaat ccggcgaaaa aactgctgcg tgatattggt ttgaaactgg
    29521 ccgacacgct tcctggcgtt aagccgcaac ttatccgcca ggcaatggga ttaaacgatt
    29581 tgcctgaatg gctgcgttaa aaatttctcc tctgttgttt atttgatacc catcacactt
    29641 tcatctcccg gttttttcgc cgggagattt tcctcatttg aaataaacta atttcacctc
    29701 cgttttcgca ttatattttc taataccatg attttttgat ttagtgtttt ttgacatttt
    29761 tttagctctt aatattgtct tattcaaatt gactttctca tcacatcatc tttgtataga
    29821 aattggtgta ttttttggtt ttttattctg tcgcgatttt tgcatttttt aaccataagc
    29881 taatgtgatg atcaatttta ccttatggtt aacagtctgt ttcggtggta agttcaggca
    29941 aaagagaacg attgcgttgg ggaccgggag tggctccgat gctgggtttt gtggtgataa
    30001 tttcaccatg aaaaagttgt cagccccgct tattcaatga ggacaagatg gcacaacaga
    30061 ctcctttgta cgaacaacac acgctttgcg gcgctcgcat ggtggatttc cacggctgga
    30121 tgatgccgct gcattacggt tcgcaaatcg acgaacatca tgcggtacgt accgatgccg
    30181 gaatgtttga tgtgtcgcat atgaccatcg tcgatctccg cggcagccgc acctgggagt
    30241 ttctgcgtta tctgctggcg aacgatgtgg cgaagctcac caaaagcggc aaagcccttt
    30301 actcggggat gttgaatgcc tctggcggtg tgatagatga tctcatcgtc tactacttta
    30361 ctgaagattt cttccgcctc gttgttaact ccgccacccg cgaaaaagac ctctcctgga
    30421 ttacccaaca cgctgaacct ttcggcatcg aaattaccgt tcgtgatgac ctttccatga
    30481 ttgccgtgca agggccgaat gctcaggcaa aagctgccac actgtttaat gatgctcagc
    30541 gtcaggcggt ggaagggatg aaaccgttct ttggcgtgca ggtgggcgat ctgtttattg
    30601 ccaccactgg ttataccggt gaagcgggct atgaaattgc gctgcccaat gaaaaagcgg
    30661 ccgatttctg gcgtgcgctg gtggaagcgg gcgttaagcc ctgcggtctg ggggcgcgtg
    30721 acacgctgcg tctggaagcg ggcatgaatc tttatggtca ggagatggac gaaactattt
    30781 ctcctttagc cgccaacatg ggctggacta tcgcctggga accgacagat cgtgacttta
    30841 tcggtcgtga agccctggaa gtgcagcgtg agcatggtac agaaaaactg gttggtctgg
    30901 tgatgaccga aaaaggcgtg ctgcgtaatg aactgccggt acgctttacc gatgcgcagg
    30961 gcaaccagca tgaaggcatt atcaccagcg gtactttctc cccgacgctg ggttacagca
    31021 ttgcgctggc gcgcgtgccg gaaggtattg gcgaaacggc gattgtgcaa attcgcaacc
    31081 gtgaaatgcc ggttaaagtg acaaaacctg tttttgtgcg taacggcaaa gccgtcgcgt
    31141 gatttacttt tttggagatt gattgatgag caacgtacca gcagaactga aatacagcaa
    31201 agaacacgaa tggctgcgta aagaagccga cggcacttac accgttggta ttaccgaaca
    31261 tgctcaggag ctgttaggcg atatggtgtt tgttgacctg ccggaagtgg gcgcaacggt
    31321 tagcgcgggc gatgactgcg cggttgccga atcggtaaaa gcggcgtcag acatttatgc
    31381 gccagtaagc ggtgaaatcg tggcggtaaa cgacgcactg agcgattccc cggaactggt
    31441 gaacagcgaa ccgtatgcag gcggctggat ctttaaaatc aaagccagcg atgaaagcga
    31501 actggaatca ctgctggatg cgaccgcata cgaagcattg ttagaagatg agtaacggct
    31561 ttattcctct tctgcgggag aggatcaggg tgaggaaaat ttatgcctca ccctcactct
    31621 cttcgtaagg agagaggttc acaattcact gcacgtttca ggaaccatcg ctcatgacac
    31681 agacgttaag ccagcttgaa aacagcggcg cttttattga acgccatatc ggaccggacg
    31741 ccgcgcaaca gcaagaaatg ctgaatgccg ttggtgcaca atcgttaaac gcgctgaccg
    31801 gccagattgt gccgaaagat attcagcttg cgacaccacc gcaggttggc gcaccggcga
    31861 ccgaatacgc cgcactggca gaactcaagg ctattgccag tcgcaataaa cgcttcacgt
    31921 cttacatcgg catgggttac accgccgtgc agctaccgcc ggttatcctg cgtaacatgc
    31981 tggaaaatcc gggctggtat accgcgtaca ctccgtatca gcctgaagtc tcccagggcc
    32041 gccttgaagc actgctcaac ttccagcagg taacgctgga tttgactgga ctggatatgg
    32101 cctctgcttc tcttctggac gaggccaccg ctgccgccga agcaatggcg atggcgaaac
    32161 gcgtcagcaa actgaaaaat gccaaccgct tcttcgtggc ttccaatgtg catccgcaaa
    32221 cgctggatgt ggtccgtact cgtgctgaaa cctttggttt tgaagtgatt gtcgatgacg
    32281 cgcaaaaagt gctcgaccat caggacgtct tcggcgtgct gttacagcag gtaggcacta
    32341 ccggtgaaat tcacgactac actgcgctta ttagcgaact gaaatcacgc aaaattgtgg
    32401 tcagcgttgc cgccgatatt atggcgctgg tgctgttaac tgcgccgggt aaacagggcg
    32461 cggatattgt ttttggttcg gcgcaacgct tcggcgtgcc gatgggctac ggtggcccac
    32521 acgcggcatt ctttgcggcg aaagatgaat acaaacgctc aatgccgggc cgtattatcg
    32581 gtgtatcgaa agatgcagct ggcaataccg cgctgcgcat ggcgatgcag actcgcgagc
    32641 aacatattcg tcgtgagaaa gcgaactcca acatttgtac ttcccaggta ctgctggcaa
    32701 acatcgccag cctgtatgcc gtttatcacg gcccggttgg cctgaaacgt atcgctaacc
    32761 gcattcaccg tctgaccgat attctggcgg cgggcctgca acaaaaaggt ctgaaactgc
    32821 gccatgcgca ctatttcgac accttgtgtg tggaagtggc cgacaaagcg ggcgtactga
    32881 cgcgtgccga agcggctgaa atcaacctgc gtagcgatat cctgaacgcc gtggggatca
    32941 cccttgatga aaccaccacg cgtgaaaacg taatgcagct tttcagtgtg ctgctgggcg
    33001 ataaccacgg gctggagatc gacacgctgg acaaagacgt ggctcacgac agccgctcta
    33061 tccagcctgc gatgctgcgc gacgacgaaa tcctcaccca tccggtgttt aatcgctatc
    33121 acagcgaaac cgaaatgatg cgctatatgc actcgctgga gcgtaaagat ctggcgctga
    33181 atcaggcgat gatcccgctg ggttcctgca ccatgaaact gaacgccgcc gccgagatga
    33241 tcccaatcac ttggacggaa tttgccgaac tgcacccgtt ttgcccgccg gaacaggctg
    33301 aaggttatca gcagatgatt gcgcagctgg ctgactggct ggtgaaactg accggttacg
    33361 acgccgtttg tatgcagccg aactccggcg cacagggcga atacgcgggc ctgctggcga
    33421 ttcgtcatta tcatgaaagc cgcaacgaag ggcatcgcga tatctgcctg atcccggctt
    33481 ctgcgcacgg aactaacccc gcttctgcac atatggcagg aatgcaggtg gtggttgtgg
    33541 cgtgtgataa aaacggcaac atcgatctga ctgatctgcg cgcgaaagcg gaacaggcgg
    33601 gcgataacct ctcctgtatc atggtgactt atccttctac ccacggcgtg tatgaagaaa
    33661 cgatccgtga agtgtgtgaa gtcgtgcatc agttcggcgg tcaggtttac cttgatggcg
    33721 cgaacatgaa cgcccaggtt ggcatcacct cgccgggctt tattggcgcg gacgtttcgc
    33781 acctcaacct gcataaaact ttctgcattc cgcacggcgg tggtggtccg ggtatgggac
    33841 cgatcggtgt gaaagcgcat ctggcaccgt ttgtaccggg tcatagcgtg gtgcaaatcg
    33901 aaggcatgtt aacccgtcag ggcgcagttt ctgcggcacc gttcggtagc gcctctatcc
    33961 tgccaatcag ctggatgtac atccgcatga tgggcgcaga agggctgaaa aaagcaagcc
    34021 aggtggcaat cctcaacgcc aactatattg ccagccgcct gcaggatgcc ttcccggtgc
    34081 tgtataccgg tcgcgacggt cgcgtggcgc acgaatgtat tctcgatatt cgcccgctga
    34141 aagaagaaac cggcatcagc gagctggata ttgccaagcg cctgatcgac tacggtttcc
    34201 acgcgccgac gatgtcgttc ccggtggcgg gtacgctgat ggttgaaccg actgaatctg
    34261 aaagcaaagt ggaactggat cgctttatcg acgcgatgct ggctatccgc gcagaaattg
    34321 accaggtgaa agccggtgtc tggccgctgg aagataaccc gctggtgaac gcgccgcaca
    34381 ttcagagcga actggtcgcc gagtgggcgc atccgtacag ccgtgaagtt gcggtattcc
    34441 cggcaggcgt ggcagacaaa tactggccga cggtgaaacg tctggatgat gtttacggcg
    34501 accgtaacct gttctgctcc tgcgtaccga ttagcgaata ccagtaattc actgattcga
    34561 gtattttcta aaggcgcttc ggcgcctttt tagtcagatg acaaagtaca aaagtgctca
    34621 gacagtcccc tcgccccttt ggggagaggg ttagggtgag gggaacaggc cggcactggc
    34681 gcgaatattt accctcaccc cgaccctctc cctgaaaggg cgagggggaa aagcgtgcca
    34741 acattgaaga ttgagccagt ttgttagcaa tctcaaagat acgtcaacga attaattttt
    34801 ctcggaaaaa caaatggcta tagcacttgt gaccggtggc agtcgcggca tcgggcgggc
    34861 aactgcatta ctgttggcgc aagaagggta tacggtggcg gttaattatc agcaaaacct
    34921 ccacgcggcg caggaagtga tgaacttaat aatgcaagcc ggtggcaaag cattcgtgct
    34981 ccaggcggat atcagcgacg aaaaccaggt cgttgcgatg tttacagcaa tcgatcagca
    35041 cgatgaaccg ctagcagcgc tggtcaataa cgccgggatc ttgtttaccc agtgcaccgt
    35101 tgaaaacctt accgcagagc gaatcaaccg agtactttcc accaacgtga cgggatattt
    35161 tctctgctgc cgcgaggcgg taaaacgcat ggcgcttaaa aatggtggca gtggcggcgc
    35221 tatcgtcaat gtctcttcgg tggcctcacg gttgggttcg ccaggggaat atgttgatta
    35281 cgcggcatcg aaaggggcga ttgattcgtt aaccaccgga ctatcgctgg aagtcgccgc
    35341 gcaggggatc cgcgttaact gcgtgcggcc agggtttatt tataccgaaa tgcacgccag
    35401 cggcggcgag cctggacgcg tcgatcgcgt taagtcgaac atccccatgc agcgtggtgg
    35461 acaggcagaa gaggtcgcgc aggccattgt ctggctacta agtgataaag cctcttacgt
    35521 cacgggaagt tttatcgatt tggcgggcgg gaaataaaac agggaagttg tctgaccgga
    35581 tgcaacaagt attgcatccg gtacttcatc gacttaaagc ttctcgccgt tgctggcaat
    35641 cacctctttg taccagttaa agctcttctt acgtgaacgc gacatatcgc cagtaccgtc
    35701 gtcatgttta ttcacataga taaagccgta gcgtttgctg tactgcccgg tagtgaatga
    35761 cacgcagtcg atgcagcccc acggtgtgta gcccatcaga tccacaccat cgtaagtcac
    35821 tgcttttttc atctcttcaa tatgggcgcg caggtagtca atgcggtagt cgtcgttgat
    35881 gctgccatct tcttccactt tgtcgtaagc gccaaaaccg ttttcgacaa taaacagcgg
    35941 cctctgataa cgctcataca gttcgcaaag ggcatagcgc agacctactg gatcaatctg
    36001 ccagccccag tcagacgctt taacatacgg gttcggtacg ctgccttcaa aaccagagat
    36061 cgcatcgccg gtgccgcctt cggccttcac tgcgttggtc atgtaatagc tgaaaccaag
    36121 ataatcgcag gtgccttcgc gcagtacatc gagatcgccg tcttccattt tgatattaaa
    36181 tccgcgacgt tcccactcgt tcaatacata ggacgggtaa tagccgcata gctgcacatc
    36241 ggtaaagacg tagcgttcgc gcatcgactc ctgagcgaac atcacatcgt ccgggttaca
    36301 tgagtaggga tagagcggca ccatcgccag catacagccg actttcatct ccgggttaat
    36361 gcgacgcgcg gctttcactg ccagggcgct ggcgacaaac tggtgatgca gcacctggta
    36421 catcgtctct tccgggttgt catgctcggt atacaccacg ccggagcagc agtaaccaaa
    36481 cagcggcgca cgccagttac gctggttgtt tatttcgttg aaggtcatcc agtatttgac
    36541 tttatgcttg tagcgttcga ataccacttc cgcgaaacgg acaaagaaat caacgaattt
    36601 acggttggtc cagctaccgt attgctgcac cagatgcagc ggcatttcaa agtgggagag
    36661 ggttatcacc ggttcgatgt tgtatttcag cagttcatcg aacagagagt cgtaaaactt
    36721 cagcccttct tcgtttggct gagcttcatc accttttgga aaaatacgcg tccaggcgat
    36781 ggaagtccga aaacatttga agcccatttc ggcaaatagc ttgatgtctt ccttgtagtg
    36841 accataaaaa tcaacggctt catggtttgg atagtatttt cctggcaaga cttctttggt
    36901 gatttcgcgc ggcacgccgt gtgcgccacc ggtcagaacg tcacaaatgc tcggtccttt
    36961 gccgcctttg ttccagccgc cttcgacctg atgagcggca acggcgccac cccataagaa
    37021 atctttcggt aaggtgagtt ttttcactat cataggctcc agatcagtca attcttacag
    37081 gaaatagtac catgcagcca aacgacatca cttttttcaa cgtttccagg atgacattct
    37141 ggctgggcgt aaaaccatca ccatccgcga cgagtctgaa tcgcacttca aaacgggtga
    37201 tgtgcttcgt gtcggacgtt ttgaagatga cggttatttt tgcacgattg aagtcaccgc
    37261 aacctcaacc gtaacgctgg atacactgac ggaaaaacat gcagagcagg aaaatatgac
    37321 cctgcctgaa ctgaaaaagg tcattgctga catctatccc ggtcagacac aattttatat
    37381 gattgaattt aaatgtcttt aaattgaact cacgcctgtt agttgaaatt tcgcatttaa
    37441 tttcatgatt tttaggagat tagtctcaaa tcagaattta ttttagctaa caggtgttca
    37501 ctggaactat tctcagttac gctggagagg tatacatgtg cgtgtacgtt tctctggagt
    37561 aagttatggt tcagaagccc ctcattaagc agggatattc gctggcagag gaaattgcca
    37621 acagcgtcag tcacggcatt gggctggtgt ttggtatcgt tgggctggtg ttgctactgg
    37681 ttcaggcggt ggatctcaat gccagcgcca cagcgataac cagttacagc ctctatggcg
    37741 gcagtatgat cctgctgttc ctcgcttcga cgctctatca cgccattcct catcaacggg
    37801 caaaaatgtg gctgaagaaa tttgaccatt gcgctattta cctgttgatt gccggaacct
    37861 acacgccgtt tttgctggtg gggctggatt ctccgttagc gcgcgggttg atgattgtta
    37921 tctggagcct agcattgctg gggattctgt tcaaactgac catcgcgcac cgattcaaaa
    37981 ttttatctct ggtgacttat ctggcgatgg gctggctgtc gctggtggta atttatgaaa
    38041 tggcagttaa gctcgcggcg ggcagcgtta ccttactggc ggtaggcggc gtggtttatt
    38101 cgctcggggt gattttctac gtctgcaaac gcattccata caaccatgcc atctggcacg
    38161 gcttcgtgct cggcggtagt gtgtgccact ttctggcgat ctatttgtat attgggcagg
    38221 cgtaatgggt atgcctttgt aggcctgata agacgcgtca gcgtcgcatc aggcatcgga
    38281 gcgcaaccgc cggatgcggc gtgaacgcct tatccgatct acgggcctcg tccctgttgt
    38341 gataaaggca tcaaatccca gacatgataa gacgcgtcag cgtcgcatca ggcgataaat
    38401 gcctgatgcg atacagagat ttactcttcg agcgaatacg gcagcggctc gatatgcagt
    38461 gtattcgcat cgtcgcgtac gcggaagatg ctgtccggtt ccatatcgtt attcatgacg
    38521 acctgtacca cgacctgacc atcttccagt tttaccgcag ccagcaccgt accggtacga
    38581 cgccagttct cgcccatttt cagctctaag tcttcaccag cttccggcag tcggctggca
    38641 ctacctgcca gcaaccagag cgcgcgtttg ttggcaccac ggaattttgc tcgcgccacc
    38701 atctcttgtc cggtataaca gcctttctta aagctgatac cgcccagcgc ctggagattg
    38761 gtcgcctgtg ggataaactg cccgctgttg gcggcatcaa tcaccgggaa accagcttca
    38821 atgtttaatg ccagccactg ttggctattg ttcagttccg cttcaccgcg cagtttatcg
    38881 gtcagcatat tagcagtagc ttcatcggtt acgatcagga aacgttctgc cgggtgttca
    38941 aaccatagca aagtggtcgc gccttctttg actacctgtt tttctttcga aggcagttcg
    39001 ctaaagagat ttgccagcgc ggcgcgcgcc tgaaaaccgg caacaccaag cagcacacgc
    39061 tcgtcgtctg gcgcgatggt cactttagag aataccgcat attttttcag ttcagtcagc
    39121 tgcggttcac gcacgctgcg ccgttcaatc catgcaaagc catcgccgtc gcggaacaga
    39181 cgtaaattgc tccacatttt acctttggcg tcgcaatggg cggcgagcag gtgctgatct
    39241 tctgccatct ggctgacatc tgctgtcacc tgaccctgca tatatttttc gctgtccgcg
    39301 ccagtaatgg tggcaagcgc ccagtcatca agcgtcatca gcgtcagcgg taaacgggca
    39361 gaagccgtag gctgacgtgg aggaaaaggt gtaaaagcca taatagagtc ctgagttgct
    39421 taacggcgta ttaatgacta atggtaaaag agctatgagg caatgcaagt gctttcacta
    39481 cgcgatctgt gccttctgcg aggcgactgc caaaaaaatg aagcccttga attgttgtga
    39541 attaatttcg cgatcgcgat ggtgtttact gatattcccg taaaagaagt cgaaaaacac
    39601 gttacaatag ccgggtactg gattttcgtg agacaggaag aagatggaca ttaacaacaa
    39661 agcccgaatt cattgggcat gccgccgtgg tatgcgcgaa ctcgatattt caatcatgcc
    39721 gtttttcgaa catgagtacg acagcttaag cgatgacgaa aaacgcatct ttattcgtct
    39781 gctggaatgt gacgatccgg acctgtttaa ctggctgatg aatcacggta aaccggccga
    39841 tgcagaactg gaaataatgg tccgactcat ccagacacgg aaccgggaac gtggtcctgt
    39901 ggcaatctga tttgcgcgtc tcctggcgcg cacagtggct ttccttgctg attcatgggc
    39961 tggttgccgc tgttatttta ctcatgccct ggccgctcag ttacaccccg ttatggatgg
    40021 tgttactttc gctggtggta tttgattgcg ttcgcagcca gcggtgtatt aatgctcgcc
    40081 agggggaaat tcgcttgttg atggacgggc gtttgcgttg gcaagggcag gagtggagca
    40141 tcgtcaaagc accgtggatg attaagagcg gcatgatgct gcgtttacgt tctgatggcg
    40201 gtaaacggca acatttatgg ctggcagccg acagcatgga cgaagccgaa tggcgggatt
    40261 tacggcggat tttgttgcaa caagagacgc aaagataaag atgacggcaa agcatatgcg
    40321 ctaagccgtc agtgagatca ggcgtaatgc tctgccattt cgttgaggat ttgctcgcac
    40381 cagctctgaa tacgctcgtc gctaaggtca tactggttag tttcatccag cgccagaccc
    40441 acgaacagtt gcccgtcagc aatcaccggt ttcgggctgg taaattcata tccttccgtt
    40501 ggccagtagc cgacgaactt cacgcctttg gtcgagagtt tgtcatgcag cataccgagc
    40561 gcatcgagga accactcgcc gtatcccagt tgatcgccaa gcccatacag cgcaataatt
    40621 ttaccttcaa ggttcaggtc gtcgagctga tcccagacgg cttcccagtc ttcctggatt
    40681 tcaccaaaat cccaggtcgg gatacccaga atgagcacat cgtactgctc cattaatttc
    40741 ggggagtcgt ccttgaggtt atgtaaggtc accagttctg ggccgataat atcgcggatt
    40801 ttttctgccg ccatttcggt gtaacaggtg ctggaaccgt aaaaaagacc catattcata
    40861 acgtaagctt ctcgattctg ctgtggcttt tgcagctagt gtatcagaat cgctttagtc
    40921 acggcacaat aaggcataat ggatcaaatg agtgaccata aggggcgcaa gtgaaacagg
    40981 agctggcacg catcgagcag tttcttgatg ctctgtggct ggaaaaaaat ctggctgaaa
    41041 atacgttgaa cgcttaccgt cgcgatctgt caatgatggt ggagtggttg catcaccgcg
    41101 ggttgacgct ggcgacggcg caaagtgacg atttgcaggc attactggca gaacggctgg
    41161 agggcgggta taaagccacc agctcagcgc gtttgctgag tgcagtgcgc cgattgttcc
    41221 agtatcttta tcgcgaaaag tttcgtgaag acgatcccag tgcgcatctc gcttcaccga
    41281 aattgcccca gcgtttgcca aaagatttaa gcgaagcgca ggtcgaacgt ttattacagg
    41341 cgccattaat tgatcagcca ctggagctac gcgataaagc catgcttgaa gtgttgtatg
    41401 ctaccgggct gcgtgtctct gaactggtcg ggctgacaat gagtgatatc agcctgcgtc
    41461 agggcgtagt gcgggtcatt ggtaaaggca acaaagagcg cctggtgccg ttaggggaag
    41521 aggcggttta ctggctggaa acctatctgg aacatgggcg tccgtggctg ctgaatggtg
    41581 tgtcaattga cgtgttgttt cccagccagc gtgcgcagca gatgacgcga cagaccttct
    41641 ggcaccgcat taaacattat gccgtgctgg cgggtatcga cagcgaaaag ctgtcaccgc
    41701 atgtgttgcg tcacgctttt gccactcatt tattaaatca tggtgcggat ttacgcgtgg
    41761 tgcagatgct actgggccac agcgatcttt ccaccacgca aatttatacg catgtcgcta
    41821 ccgagcgtct gcggcaactt catcaacagc atcacccgcg ggcgtgatgt ctgaaaagaa
    41881 cgggaagatt tatgaagaaa ggttttatgt tgtttacttt gttagcggcg ttttcaggct
    41941 ttgctcaggc tgatgacgcg gcaattcaac aaacgttagc caaaatgggc atcaaaagca
    42001 gcgatattca gcccgcgccc gtagctggca tgaagacagt tctgactaac agcggtgtgt
    42061 tgtacatcac cgatgatggt aaacatatca ttcaggggcc aatgtatgac gttagtggca
    42121 cggctccggt caatgtcacc aataagatgc tgttaaagca gttgaatgcg cttgaaaaag
    42181 agatgatcgt ttataaagcg ccgcaggaaa aacacgtcat caccgtgttt actgatatta
    42241 cctgtggtta ctgccacaaa ctgcatgagc aaatggcaga ctataacgcg ctggggatca
    42301 ccgtgcgtta tcttgctttc ccgcgccagg ggctggacag cgatgcagag aaagaaatga
    42361 aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga tgatgtgatg gcaggtaaaa
    42421 gcgtcgcacc agccagctgc gacgtggata ttgccgacca ttacgcactt ggcgtccagc
    42481 ttggcgttag cggtactccg gcagttgtgc tgagcaatgg cacacttgtt ccgggttacc
    42541 agccgccgaa agacatgaaa gaattcctcg acgaacacca aaaaatgacc agcggtaaat
    42601 aattcgcgtg aaacaacaga tacaacttcg tcgccgtgaa gtcgatgaaa cggcagactt
    42661 gcccgctgaa ttgcctccct tgctgcgccg tttatacgcc agccggggag tacgcagtgc
    42721 gcaagaactg gaacgcagtg ttaaaggtat gttgccctgg cagcaactaa gcggtgtcga
    42781 aaaggccgtt gagatccttt acaacgcttt tcgcgaagga acgcggatta ttgtggtcgg
    42841 tgatttcgac gccgacggcg cgaccagcac ggctctaagc gtgctggcga tgcgctcgct
    42901 tggttgcagc aatatcgact acctggtacc aaaccgtttc gaagacggtt acggcttaag
    42961 cccggaagtg gtcgatcagg cccatgcccg tggcgcgcag ttaattgtca cggtggataa
    43021 cggtatttcc tcccatgcgg gagttgagca cgctcgctcg ttgggcatcc cggttattgt
    43081 taccgatcac catttgccgg gcgacacatt acccgcagcg gaagcgatca ttaaccctaa
    43141 cttgcgcgac tgtaatttcc cgtcgaaatc actggcaggc gtgggtgtgg cgttttatct
    43201 gatgctggcg ctgcgcacct ttttgcgcga tcagggctgg tttgatgagc gcggcatcgc
    43261 aattcctaac ctggcagaac tgctggatct ggtagcactg gggacagtag cggacgtcgt
    43321 gccgctggac gctaataatc gcattctgac ctggcagggg atgagtcgca tccgtgccgg
    43381 aaagtgccgt ccagggatta aagcgctgct ggaagtggca aatcgcgatg cacaaaaact
    43441 agccgccagc gatttaggtt ttgcgctggg accgcgtctc aatgctgccg ggcgactgga
    43501 tgatatgtcc gttggtgtgg cgctgttgct gtgcgacaac atcggcgaag cgcgcgtgct
    43561 ggcaaatgaa ctcgatgcgc taaaccagac gcgaaaagag atcgaacaag gaatgcaggt
    43621 tgaagccctg accctgtgcg agaaactgga gcgcagccgt gacacgctac ccggcgggct
    43681 ggcaatgtat caccccgaat ggcatcaggg cgttgtcggt attctggctt cgcgtatcaa
    43741 agagcgtttt caccgtccgg ttatcgcctt tgcgccagca ggtgacggta cgctgaaagg
    43801 ttccggtcgc tccattcagg ggctgcatat gcgtgatgcg ctggagcgat tagacacact
    43861 ctaccccggc atgatgctca agtttggcgg tcatgcgatg gcggcgggtt tgtcgctgga
    43921 agaggataaa ttcgaactct ttcaacaacg gtttggcgaa ctggttactg agtggctggc
    43981 cccttcgcta ttgcaaggcg aagtggtgtc agacggtccg ttaagcccgg ccgaaatgac
    44041 catggaagtg gcgcagctgc tgcgcgatgc tggcccgtgg gggcagatgt tcccggagcc
    44101 gctgtttgac ggtcatttcc gtctgctgca acagcggctg gtgggcgaac gtcatttgaa
    44161 ggtgatggtc gaaccggtcg gcggcggtcc actgctggat ggtattgctt ttaatgtcga
    44221 taccgctctc tggccggata acggcgtgcg cgaagtgcaa ctggcttata agctcgatat
    44281 caacgagttt cgcggcaacc gcagcctgca aattatcatc gacaatatct ggccaattta
    44341 gcgtcatctt ctctataaaa aagagcgtgg attgggtaca atcccgctct tatcaccgca
    44401 ttttgactag ctcaataaaa gaaatcagac catgtttgaa attaatccgg taaataatcg
    44461 cattcaggac ctcacggaac gctccgacgt tcttaggggg tatctttgac tacgacgcca
    44521 agaaagagcg tctggaagaa gtaaacgccg agctggaaca gccggatgtc tggaacgaac
    44581 ccgaacgcgc acaggcgctg ggtaaagagc gttcctccct cgaagccgtt gtcgacaccc
    44641 tcgaccaaat gaaacagggg ctggaagatg tttctggtct gctggaactg gctgtagaag
    44701 ctgacgacga agaaaccttt aacgaagccg ttgctgaact cgacgccctg gaagaaaaac
    44761 tggcgcagct tgagttccgc cgtatgttct ctggcgaata tgacagcgcc gactgttacc
    44821 tcgatattca ggcggggtct ggcggtacgg aagcacagga ctgggcgagc atgcttgagc
    44881 gtatgtatct gcgctgggca gaatcgcgtg gtttcaaaac tgaaatcatc gaagagtcgg
    44941 aaggtgaagt ggcgggtatt aaatccgtga cgatcaaaat ctccggcgat tacgcttacg
    45001 gctggctgcg tacagaaact ggcgttcacc gcctggtgcg taaaagcccg tttgactccg
    45061 gcggtcgtcg ccacacgtcg ttcagctctg cgtttgttta tccggaagtt gatgatgata
    45121 ttgatatcga aatcaacccg gcggatctgc gcattgacgt ttatcgcgcg tccggcgcgg
    45181 gcggtcagca cgttaaccgt accgaatctg cggtgcgtat tacccacatc ccgaccggga
    45241 tcgtgaccca gtgccagaac gaccgttccc agcacaagaa caaagatcag gccatgaagc
    45301 agatgaaagc gaagctttat gaactggaga tgcagaagaa aaatgccgag aaacaggcga
    45361 tggaagataa caaatctgac atcggctggg gcagccagat tcgttcttat gtccttgatg
    45421 actcccgcat taaagatctg cgcaccgggg tagaaacccg caacacgcag gccgtgctgg
    45481 acggcagcct ggatcaattt atcgaagcaa gtttgaaagc agggttatga ggaaccaaca
    45541 tgtctgaaca acacgcacag ggcgctgacg cggtagtcga tcttaacaat gaactgaaaa
    45601 cgcgtcgtga gaagctggcg aacctgcgtg agcagggaat tgccttcccg aacgatttcc
    45661 gtcgcgatca tacctctgac caattgcacg cagaattcga cggtaaagag aacgaagaac
    45721 tggaagcgct gaacatcgaa gtcgccgttg ctggccgcat gatgacccgc cgtattatgg
    45781 gtaaagcgtc tttcgttacc ctgcaggatg tgggtggccg cattcagctg tacgttgccc
    45841 gtgacgatct gccggaaggc gtttacaacg agcagttcaa aaaatgggac ctcggcgaca
    45901 tcctcggcgc gaaaggtaaa ctgttcaaaa ccaaaaccgg cgaactgtcc atccactgta
    45961 ccgagctgcg tctgctgacc aaagcactgc gtccgctgcc ggataaattc cacggcttgc
    46021 aggatcagga agcgcgctat cgtcagcgtt atctggatct catctctaac gatgaatccc
    46081 gtaacacctt taaagtacgc tcgcagatcc tctctggtat tcgccagttc atggtgaacc
    46141 gcggctttat ggaagttgaa acgccgatga tgcaggtgat cccaggcggt gccgctgcgc
    46201 gtccgtttat cacccaccat aacgcgctgg atctcgacat gtacctgcgt atcgcgccgg
    46261 aactgtacct caagcgtctg gtggtcggtg gcttcgagcg tgtattcgaa atcaaccgta
    46321 acttccgtaa cgaaggtatt tccgtacgtc ataacccaga gttcaccatg atggaactct
    46381 acatggctta cgcagattac aaagatctga tcgagctgac cgaatcgctg ttccgtactc
    46441 tggcacagga tattctcggt aagacggaag tgacctacgg cgacgtgacg ctggacttcg
    46501 gtaaaccgtt cgaaaaactg accatgcgtg aagcgatcaa gaaatatcgc ccggaaaccg
    46561 acatggcgga tctggataac ttcgactctg cgaaagcgat tgctgaatct atcggtatcc
    46621 acgttgagaa gagctggggt ctgggccgta tcgttaccga gatcttcgaa gaagtggcag
    46681 aagcacatct gattcagccg accttcatta ctgaatatcc ggcagaagtt tctccgctgg
    46741 cgcgtcgtaa cgacattaac ccggaaatca cagaccgctt tgagttcttc atcggtggtc
    46801 gtgaaatcgg taacggcttt agcgagctga atgacgcgga agatcaggcg caacgcttcc
    46861 tggatcaggt tgccgcgaaa gacgcaggtg acgacgaagc gatgttctac gatgaagatt
    46921 acgttaccgc actggaacat ggcttaccgc cgacagcagg tctggggatt ggcatcgacc
    46981 gtatggtaat gctgttcacc aacagccata ccatccgcga cgttattctg ttcccggcga
    47041 tgcgtccggt gaaataagca ttacgttatg ctcacaaccc cggcaaatgt cggggttttt
    47101 tatttaagct gggtaaatgc agataatcgt attctggctt cgcgatttgt cgcctgcatc
    47161 accatccacg gactgaacgc ccacggcgtg gcatcaatac cgcgtaatac atcagctaaa
    47221 tcacaccatt gataatccat cacttcatca tcgttgatct gtaacgcact ggtggtgcgt
    47281 gcggcaaata ccggacacac ttcattttcc acaatgcctc tcggatcggt ggcgcggtag
    47341 cgaaagtcag gatagataga ttcaggaggc gtaatttcca cgccaagctc ataacggcaa
    47401 cggcggatca ccgcgtcttc gctgctttct cccagttgtg ggtgcccaca aaccgagtta
    47461 gtccacacgc caggccacgc ttttttgctc agtgcgcggc gggtaactaa taattgcccc
    47521 ttggcattaa acagccaact ggagaacgcg agatgtaaga gggtgtctgc cgtgtgtgcg
    47581 gcatactttt ccagcgtacc cgtgggaact ccctgtgcat tcaataaaat gacgtgttcc
    47641 gtttgcataa tttctcacat gtaattctga tcactcatca ttatagataa ttgaagagtg
    47701 aaatgattaa ccaagagagc gtttatcgac aaccgcccgt tttgagcgga tgccgacagg
    47761 taaaaaccat ttaaaaaagt agcatcatat atttgaagcg actttcaatg gccattgcaa
    47821 cacagtcaga aatgacagga atagagagag taaaaagcaa caacagactg aatatagcgc
    47881 gcattaaaaa attcatatta ttctccatgc tcatttttct tcagcttgcg catcagaagc
    47941 cacatacgta ctgtcctgac ttctaaatct ttatcagaga cggattcctt caccaggtca
    48001 gcatgatgct gatagccatt aaagaagacg ttcagcacaa cggcgctaag ggttgcgagc
    48061 ataatgccgc tatgtagcag cggttgcagt acggccggta acttagaaaa gaaatcgtga
    48121 gagagcgtcg gagtcatgcc aacgccgaga ctgatcgcca caatatagag gttgtaacgg
    48181 ttggtggtgt agttacagcg cgacagaatt cgaatccctg tcgccagtac catgccgaac
    48241 atcactagac cagcgccgcc cagcacaaat tgcggaatgg aggctaccag caccgccatt
    48301 tttggcacca tgccaaacag gattaatata attcccgaag aaatacacac ccagcggcta
    48361 tgaacgcgca tcacgctaac caggccaacg ttttgagaaa atgacgtgtg ggggaagctg
    48421 ttaaacgtgc cgcctatcat tgtccctacg ccatcgacac gcagcccgca aataatatcg
    48481 tgtgaagaga gtttacgacc gactatttca cccagtgcca ggaacatccc catcgactcg
    48541 ataaacacga tgattaacac ggcagtcatg gtcagaatgg aaacgggatc gaaaatcggc
    48601 ataccaaacg acatcggggt gacaatcgca aaccacgagg catcatgtag cccggataaa
    48661 ttgacttcat tcatcatcca cgaaagtaaa aagccaaata caatccccag taatacggcg
    48721 acgttggaca taaatccttt cgcatagcga gtaatgagca agataaaaat taagacggca
    48781 aaggagatac ctaaataaac gggattacca tattgcggat tccctttacc tccagcggcc
    48841 cagtcaatac ccacctgaat gatgctaagc ccaatagaag taataaccac accggtaacc
    48901 agtggcggga ataaaggcat caagcgacca ataagtggcg ctaataatgt ggtgataaaa
    48961 cccgcggcga tagtggcacc aaatatcccc agcaggccga tatccgggtt catcccaatg
    49021 gctatcattg gtgttacagc agcaaaggtc accgacataa tcaccggcag gcggatcccc
    49081 ataaagcggc cgataccgat acattgcaat aatgtgacga tcccgcagca aaagagatcc
    49141 gagctaatga gcatcgcaat agcatctttt gagaggccca gtcggtcacc aatcataaga
    49201 ggaacagcga ctgcacctgc gtacatgaca aggacgtgtt gcagaccgag tatgatcagc
    49261 tttcctggtg ataatatgcg gtcaacctca tcagttgggc ggtcttgccc agaagatgag
    49321 ggaagttggg aatctatggc gctcatcagt gtttctcctt tcgcccgcca gaataaaaag
    49381 caaccgttta cgttaatgaa actgtctaaa cacatgctcg tggagtgcca gtttttgcga
    49441 ggaaggggag ttagagacag gaattgcgga ggcaatcaca taatcgtgct taaaccacga
    49501 aaatgacaag agaacctgac aatggaatta tctgtaattt tctcatattg atatttcatt
    49561 aatcattctg gcatgtcgca ctctcgcatt taatcgtttt tatctggata gcgctctttt
    49621 gatcggcaat gttgaaataa tgaaagcggc atagattgag gggcaataat ttgaactcag
    49681 tcgcaaaatt ataattaaaa cggtcatgct acctaatata tgcccgttta ttgcatcgcg
    49741 aaatttgaga agacataact cattaaatga aagggatgca aatgaataag tttatcgctg
    49801 ctgaagctgc ggaatgtata ggctgtca
//
LOCUS       DAOFMX010000021        49556 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.021, whole genome shotgun sequence.
ACCESSION   DAOFMX010000021 DAOFMX010000000
VERSION     DAOFMX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.021"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            105..257
                     /gene="hokA"
                     /locus_tag="QBA20_001395"
     CDS             105..257
                     /gene="hokA"
                     /locus_tag="QBA20_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HDQ6291991.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(444..656)
                     /gene="cspA"
                     /locus_tag="QBA20_001396"
     CDS             complement(444..656)
                     /gene="cspA"
                     /locus_tag="QBA20_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDQ6291992.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(937..1227)
                     /locus_tag="QBA20_001397"
                     /pseudo
     CDS             complement(937..1227)
                     /locus_tag="QBA20_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1661..2371
                     /locus_tag="QBA20_001398"
     CDS             1661..2371
                     /locus_tag="QBA20_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDQ6291993.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2421..3395)
                     /gene="ghrB"
                     /locus_tag="QBA20_001399"
     CDS             complement(2421..3395)
                     /gene="ghrB"
                     /locus_tag="QBA20_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDQ6291994.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3499..4149)
                     /locus_tag="QBA20_001400"
     CDS             complement(3499..4149)
                     /locus_tag="QBA20_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDQ6291995.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4312..6645
                     /gene="bisC"
                     /locus_tag="QBA20_001401"
     CDS             4312..6645
                     /gene="bisC"
                     /locus_tag="QBA20_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDQ6291996.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6614..7054)
                     /locus_tag="QBA20_001402"
     CDS             complement(6614..7054)
                     /locus_tag="QBA20_001402"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDQ6291997.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7051..7614)
                     /gene="tag"
                     /locus_tag="QBA20_001403"
     CDS             complement(7051..7614)
                     /gene="tag"
                     /locus_tag="QBA20_001403"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDQ6291998.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7772..8470
                     /locus_tag="QBA20_001404"
     CDS             7772..8470
                     /locus_tag="QBA20_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ6291999.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8699..9907
                     /locus_tag="QBA20_001405"
     CDS             8699..9907
                     /locus_tag="QBA20_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6292000.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10231..11922
                     /gene="eptB"
                     /locus_tag="QBA20_001406"
     CDS             10231..11922
                     /gene="eptB"
                     /locus_tag="QBA20_001406"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDQ6292001.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12014..12090
                     /locus_tag="QBA20_001407"
     tRNA            12014..12090
                     /locus_tag="QBA20_001407"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12048..12050,aa:Pro,seq:cgg)
     gene            12834..14441
                     /gene="dppA"
                     /locus_tag="QBA20_001408"
     CDS             12834..14441
                     /gene="dppA"
                     /locus_tag="QBA20_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDQ6292002.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14749..15768
                     /gene="dppB"
                     /locus_tag="QBA20_001409"
     CDS             14749..15768
                     /gene="dppB"
                     /locus_tag="QBA20_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDQ6292003.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15778..16680
                     /gene="dppC"
                     /locus_tag="QBA20_001410"
     CDS             15778..16680
                     /gene="dppC"
                     /locus_tag="QBA20_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDQ6292004.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16691..17674
                     /gene="dppD"
                     /locus_tag="QBA20_001411"
     CDS             16691..17674
                     /gene="dppD"
                     /locus_tag="QBA20_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDQ6292005.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17671..18675
                     /gene="dppF"
                     /locus_tag="QBA20_001412"
     CDS             17671..18675
                     /gene="dppF"
                     /locus_tag="QBA20_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDQ6292006.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18705..19976)
                     /locus_tag="QBA20_001413"
     CDS             complement(18705..19976)
                     /locus_tag="QBA20_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDQ6292007.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20452..20559
                     /locus_tag="QBA20_001414"
     CDS             20452..20559
                     /locus_tag="QBA20_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDQ6292008.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20935..21042
                     /gene="ldrD"
                     /locus_tag="QBA20_001415"
     CDS             20935..21042
                     /gene="ldrD"
                     /locus_tag="QBA20_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HDQ6292009.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21418..21525
                     /locus_tag="QBA20_001416"
     CDS             21418..21525
                     /locus_tag="QBA20_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDQ6292010.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21901..22008
                     /locus_tag="QBA20_001417"
     CDS             21901..22008
                     /locus_tag="QBA20_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDQ6292011.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(22095..23774)
                     /gene="bcsG"
                     /locus_tag="QBA20_001418"
     CDS             complement(22095..23774)
                     /gene="bcsG"
                     /locus_tag="QBA20_001418"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDQ6292012.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(23771..23962)
                     /gene="bcsF"
                     /locus_tag="QBA20_001419"
     CDS             complement(23771..23962)
                     /gene="bcsF"
                     /locus_tag="QBA20_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDQ6292013.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(23959..25530)
                     /gene="bcsE"
                     /locus_tag="QBA20_001420"
     CDS             complement(23959..25530)
                     /gene="bcsE"
                     /locus_tag="QBA20_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDQ6292014.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            25803..25991
                     /locus_tag="QBA20_001421"
     CDS             25803..25991
                     /locus_tag="QBA20_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDQ6292015.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            26003..26755
                     /gene="bcsQ"
                     /locus_tag="QBA20_001422"
     CDS             26003..26755
                     /gene="bcsQ"
                     /locus_tag="QBA20_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDQ6292016.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            26752..27111
                     /locus_tag="QBA20_001423"
     CDS             26752..27111
                     /locus_tag="QBA20_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HDQ6292017.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEPPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            27108..29369
                     /gene="bcsA"
                     /locus_tag="QBA20_001424"
                     /pseudo
     CDS             27108..29369
                     /gene="bcsA"
                     /locus_tag="QBA20_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            29380..31722
                     /gene="bcsB"
                     /locus_tag="QBA20_001425"
     CDS             29380..31722
                     /gene="bcsB"
                     /locus_tag="QBA20_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDQ6292018.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRRLNPDNE"
     gene            31729..32835
                     /gene="bcsZ"
                     /locus_tag="QBA20_001426"
                     /pseudo
     CDS             31729..32835
                     /gene="bcsZ"
                     /locus_tag="QBA20_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
     gene            32894..33070
                     /locus_tag="QBA20_001427"
                     /pseudo
     CDS             32894..33070
                     /locus_tag="QBA20_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            33398..35146
                     /gene="bcsC"
                     /locus_tag="QBA20_001428"
                     /pseudo
     CDS             33398..35146
                     /gene="bcsC"
                     /locus_tag="QBA20_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            35228..37216
                     /gene="hmsP"
                     /locus_tag="QBA20_001429"
     CDS             35228..37216
                     /gene="hmsP"
                     /locus_tag="QBA20_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDQ6292019.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            37402..38688
                     /gene="dctA"
                     /locus_tag="QBA20_001430"
     CDS             37402..38688
                     /gene="dctA"
                     /locus_tag="QBA20_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDQ6292020.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            38909..40405
                     /locus_tag="QBA20_001431"
     CDS             38909..40405
                     /locus_tag="QBA20_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDQ6292021.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(40501..41430)
                     /gene="kdgK"
                     /locus_tag="QBA20_001432"
     CDS             complement(40501..41430)
                     /gene="kdgK"
                     /locus_tag="QBA20_001432"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDQ6292022.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            41662..42429
                     /gene="pdeH"
                     /locus_tag="QBA20_001433"
     CDS             41662..42429
                     /gene="pdeH"
                     /locus_tag="QBA20_001433"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDQ6292023.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            42499..44559
                     /locus_tag="QBA20_001434"
     CDS             42499..44559
                     /locus_tag="QBA20_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDQ6292024.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(44741..46063)
                     /locus_tag="QBA20_001435"
     CDS             complement(44741..46063)
                     /locus_tag="QBA20_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDQ6292025.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(46474..47487)
                     /gene="yhjD"
                     /locus_tag="QBA20_001436"
     CDS             complement(46474..47487)
                     /gene="yhjD"
                     /locus_tag="QBA20_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDQ6292026.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(47536..48435)
                     /locus_tag="QBA20_001437"
     CDS             complement(47536..48435)
                     /locus_tag="QBA20_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6292027.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            48955..>49556
                     /locus_tag="QBA20_001438"
     CDS             48955..>49556
                     /locus_tag="QBA20_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDQ6292028.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT        11859 a        12385 c        13389 g        11923 t
ORIGIN      
        1 taactgtcca actttttggg gtcagtacaa agtgaggcta ccctatgcct gaacaccata
       61 aggttagcct cttactcgtt ggaaatcaac acagggggct gggaatgccg cagaaatata
      121 gattactttc tttaatagtg atttgtttca cgcttttatt tttcacctgg atgataagag
      181 attcactgtg tgaattgcat attaaacagg agagttatga gctggcggcg tttttagcct
      241 acaaattgaa agagtaagag tcttcggcgg gaaattattc ccgccttact tacggcgttg
      301 cgcattctca ttgcacccat atttattctt cacaaaaata ataatagatt ttattacgcg
      361 atcgattatt tatttcctga aaaaaaataa aaaaatcccc gccaaatggc agggatctta
      421 gattctgtgc ttttaagcag agattacagg ctggttacgt taccagctgc cgggccttta
      481 gcgccgcttt cgatggtgaa ggacactttc tgaccttcgt ccagagattt gtaaccatcg
      541 ttctggatag cagagaagtg tacgaacaca tctttagagc catcgtcagg agtgatgaag
      601 ccgaagcctt tgtcagcgtt gaaccatttt acgataccag tcattttacc ggacatagtg
      661 tattaccttt aataattaag tgtgcctttc ggcgatatgg cgtgctttac agattttgaa
      721 gcgttaaagg aatgtgcact acgaggggta tcaacgataa ctcttgaagg gacttgcctt
      781 actacactgc tttaatggtc tgtacgtcaa accgttgatg tgcattaagc cactcattgg
      841 cgggtgatgc aacaattatt tttcatattt atgattaatc ggttggaaaa cggtcctgtc
      901 atcaggaccg taaacagcaa taaagtggat aaaagtctat ttcatcaact gcttacttaa
      961 tgccgggttg gcttgaatca aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg
     1021 tctggattcc cattccttta ccatggcgac tgatacgccc aaaacccggg cgaaatcatc
     1081 gatttttaat cctgtccctt ttcataactg ctcaatttcg gtacaggacg ttgttctgtg
     1141 cgtcagggta attttctgcg tttcatcttt aaaaacaatc tgttccaggc tgctcaacag
     1201 ctcatgcatt ggatctttat attccattga aaactcctca aatcacactg cgggatcgtg
     1261 aattgcatcg aagctcatta agaatagtcg ggaaaagcag acacagcggg gtgaagtcag
     1321 ggcatgctct ggctatgatt cgattcagcc tgtcatttag cgtgacttat agccacctaa
     1381 cgatctgata aatgccgata tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc
     1441 taaccttctg cgaaggcgta aatctctggc tacaaaaaag gcaacggcac tatcattttt
     1501 taacaatgaa acaacaaatt aaccttgttt cattttctcc atgattttac caggaagatt
     1561 acgtagaaag ggtatcctgc aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt
     1621 gtgtcgttgt gcgctttttt tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg
     1681 ctctcgctgg tttgcgcctc ttgcggcgtt attaatggta gttagcctga gtgggtgttt
     1741 tgataaagaa ggcgatcagc gtaaagcgtt tatcgacttc ctgcaaaata cagtgatgcg
     1801 tagcggtgaa cgtctaccaa ccctgactgc cgatcagaaa aaacagtttg gtccttttgt
     1861 ctctgattac gcgattctgt atggttattc tcagcaggtg aatcaggcga tggattccgg
     1921 tctgcgcccg gtcgtagaca gcgttaacgc aattcgcgta ccgcaggatt acgttacgca
     1981 aagcggtccg ctgcgtgaaa tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa
     2041 tgcgaagttg caggccgatg ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc
     2101 ggtatttgat caggcattca ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt
     2161 gattccggcg gcacaaactt tcacgcaaca actggtgatg gttggagact acattgctca
     2221 gcaaggtact caggtgagct ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc
     2281 aagtgaatat aacaaactga ttgcgccatt accggcacaa catcaggcgt ttaatcaggc
     2341 ctggactaca gcagttactg caacccaata aagagtaaaa gcccgagcga ttatcgggct
     2401 ttacttcacg cagtcgcagc ttagtccgcg acgtgaggat tcacacagtt cttctcaacc
     2461 tttccttgta acgcatcaat caaattatcc acggcacagg cggccatgcc ataacgcgtc
     2521 tcatgggtgg cagatccaat atgcggtact gcgacgacgt tggccattga gagcaacggc
     2581 gaatctacgg acagtggctc ttgttcgaag acatccagcc cggcggcgtg aatttcgcct
     2641 ttctgcaatg ctgcgatcag tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg
     2701 aaaatggcgg aggatttcat tttggcgaat tgttctgcgc caaacagatg atgcgtctca
     2761 tcagttaacg gcaggatcag gcaaacgaaa tctgactctt gtaacagagt atccaaatcg
     2821 cagtagcggg cgttgaagcg ttcttctgct tctttatggt ggcggcgcgc gttatagagg
     2881 atgggcatgt tgaagccaaa gtgcacacgt tgtgccagcg ccatgccgat ccgtcccatc
     2941 ccgacaatgc ccagtgtttt atggtgaacg tcagtgccgt accagtccgg gcctatgctc
     3001 gcggtccatt cgcctgcttt tacccgttct gctacctcca caacccgacg agcggtagac
     3061 aacaccagcg ccatcagcgt atcggcgacg gtttctgtta atacggttgg cgtgtgcatc
     3121 agcagaattt ttcgggcggt aagcgcatcg acatcaaaat tgtcatagcc gacggagatc
     3181 gttgatgtgg cacgcagttt cggcattttt tccagcaatg cggcatctac attctcgttt
     3241 gaacccagta aaccttcagc ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg
     3301 ctgaggtttg ccacctggtg aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca
     3361 ggtaaggctt tgtagaggat aacggacggc ttcatgcttc tctccattca ctgataaggc
     3421 gatcacctta gcctgaaata tgatgaagga aaactgaaga atgccaggtg atttctgcat
     3481 cacctggcat gaaagggatt acagcgggct taaggtaatt tctacacggc ggttttgcgc
     3541 cttaccttct gcggtgctgt tgctggcgat tgggttagcc gggccaaggc cctgagtacg
     3601 gatgcggctg gcgtccacgc cctgggtgat caacgcgctg gcaacggaat ccgcacgctg
     3661 ctgggagaga cgcatgttca ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt
     3721 aaccgccgtt ttcggatact ctttcagcac cattgccacg ccggtcaggg tgttagcgcc
     3781 tgccggtttc aggggagcgc tgctgctgtc gaaggtcaca ttgttcggca tattgaggat
     3841 aatgttatcc ccgctgcggg ttacgctaac accagtgccg cgcattttgt cacgcagctt
     3901 cgcttcctgc acatccatgt aataacccac gccgccgccc agagctgcgc ctgctgctgc
     3961 gccaatcagc gcgcctttac cgcgatcttt cttcgaagaa gagagcgcac caatacccgc
     4021 gcccacgaga gagcccagac ctgcgccgat agcagatttg cctgcttcgc gttcgccggt
     4081 gtaagggtta gttgtgcagc cagataccgc cagagcgcca ctcactacgg cggcaagtaa
     4141 gataaacacg tttcttcatt gttaatcctt aataaccttt ttattctttg tcacgggttc
     4201 cgtggcggga gattatgccg cgtgaacatg aagattattc ctgggaatac tcggaaattt
     4261 gtaagtaata tttaactgct caatacatct aacctttcag gagtcttcgg tttggccaac
     4321 tcatcctcac gatattccgt tctgactgcc gcccactggg ggcccatgct ggttgaaacc
     4381 gacggcgaaa ccgtgtttag ctcgcgtggc gcgttagcca caggaatgga aaactccttg
     4441 cagagcgcgg ttcgcgacca ggttcacagc aatacgcggg tacgatttcc aatggtgcgc
     4501 aaaggctttc tcgcgtcacc ggaaaacccg caaggcattc gtgggcagga tgaatttgtt
     4561 cgcgtgagtt gggatgaggc gctggatctt attcaccaac aacataaacg cattcgtgag
     4621 gcttatggtc cggcatcgat ttttgctggt tcctacggct ggcgttcaaa cggcgtgctg
     4681 cataaggcct cgacattatt acaacgctat atggcgctgg caggcggtta taccgggcat
     4741 ctgggggatt attcgaccgg tgcggcacag gcgatcatgc cgtatgtcgt aggtggcagc
     4801 gaagtttatc aacagcaaac cagttggccg ctggtgctgg aacatagcga tgtcgtggtg
     4861 ttgtggagtg ctaacccact caatacgctg aaaattgcgt ggaatgcatc cgatgagcag
     4921 gggctttctt acttttccgc actgcgtgac agtgggaaaa agctgatctg cattgatcca
     4981 atgcgatcgg aaaccgtcga tttctttggc gataaaatgg agtgggtggc accgcacatg
     5041 ggcaccgatg ttgcgctgat gctggggatc gcccatacgc tggtggaaaa tggttggcac
     5101 gacgaagcgt ttctggcgcg ttgcaccaca ggttatgccg tcttcgcctc ttatttgctg
     5161 ggcgagagtg acggaatagc gaaaaacgcc gaatgggcgg cagagatttg tggtgttggc
     5221 gcagcgaaaa tccgcgagct ggcggctatt ttccaccaaa ataccaccat gctgatggca
     5281 ggctggggaa tgcagcgcca acagtttggt gagcaaaaac actggatgat cgtcacgctg
     5341 gcagcaatgt tggggcaaat cggcacaccc ggcggcggtt ttggtctttc ttaccatttt
     5401 gccaatggtg gtaaccccac gcggcgttct gcggtgctct cttccatgca gggcagcttg
     5461 ccgggtggct gcgatgcggt ggataaaatc cctgttgccc gcattgttga agcactggaa
     5521 aaccctggtg gcgcatatca acacaacggt atgaaccgac atttcccgga tattcgtttt
     5581 atctggtggg cgggcggtgc caactttact catcatcagg ataccaatcg tctgatccgt
     5641 gcctggcaaa aaccggagct ggtggtgatc tctgaatgct tctggacggc ggcggcgaaa
     5701 cacgcggata ttgttctgcc tgcgactacc tcttttgagc gtaatgatct caccatgacc
     5761 ggtgattaca gtaatcagca tctggtgccg atgaagcaag tggtgccgcc acgctatgaa
     5821 gcgcgtaatg attttgatgt ttttgccgag ttgagtgaac gttgggagaa gggcggctat
     5881 gcgcggttta cggaaggaaa aagtcagctg caatggctgg aaacgtttta taacgttgcc
     5941 cggcagcgcg gggcaagcca gcaggttgaa ttgccgccat ttgctgagtt ctggcaagcc
     6001 aaccagttaa ttgagatgcc ggaaaacccg gacagcgagc ggtttattcg cttcgctgat
     6061 ttttgccgcg atccgctggc gcatccgtta aaaaccgcca gcggcaagat tgaaatcttc
     6121 tcacagcgta ttgccgatta cggttacccg gattgccctg ggcatccaat gtggctggag
     6181 ccggacgaat ggcagggcaa tgccgaacca gaacagttgc aggtactttc tgcccatccg
     6241 gcgcaccgcc tgcacagcca gctgaattac agttctctgc gcgaattgta cgcggtagca
     6301 aaccgtgagc ctgtcaccat tcatcctgac gatgctcagg cgcgcggcat aaccgaaggg
     6361 gatatggtgc gggtgtggaa ctcccgtggg cagatccttg ccggagcggt cattagcgag
     6421 ggaattaaac ctggcgtgat ttgcattcac gaaggggcat ggccggatct ggatttaacc
     6481 gctgacggta tttgtaaaaa cggcgcagtg aacgtgctga ccaaagatct ccccagctcg
     6541 cggctgggga atggctgtgc gggtaatacg acgctggcat ggctggaaaa atacaacggt
     6601 ccggaactga cacttacagc gtttgaacca ccggccagct cataatccag gtgggtagtt
     6661 gggtttcatc ctgccatgcg caatcgacaa tgtgaaaacc ctgcgcacgg taaaaatcta
     6721 tcgccggttg atttttttga taaacctcca gcatcagatg gggatagcgc tgctgcacat
     6781 actgcatcag cgccttacca ataccgcgtc tgacggcctt cggtgcgaca aacattgctg
     6841 ccagaaatcg gccttccata atgctgacaa aaccgagaag cttaccgtcc tcttcccaga
     6901 cccagttttg cgcgttggca agataggcat cccgcaccag cggaatgcag tcacgccagt
     6961 aattcgcttt tataaaggga tgcccccagg ttgtactttc cagccacagt tcgaggatcg
     7021 cggggagttc tgaacgttgc gcttcccgaa tcatggttta tttcccagat agcaacagca
     7081 gccaaccaca tgatcattca ccagcccaca tgcctgcata aaggagtaac agattgtggt
     7141 gccgacaaac ttaaaaccac gttttttcag tgccttagat agggcgtcgg aggcggacgt
     7201 agatgtggga atttcgctca acgttgtggc ttgtgtcacc tgtggctgat gatttacaaa
     7261 cgaccagaca aagtcgggaa acggttcgcc gttctgttcc atttgcaggt acgcccgcgc
     7321 attaccaata attgcctgaa ttttccctcg atggcggata atcccggcgt cctgtaccag
     7381 tctttcgaca tcctcttcct gcattgctgc gaccttcacc ggatcgaact gatgaaagca
     7441 ggcgcgatag ttttcgcgtt ttttgaggac ggtgatccac gataacccag cctgctgccc
     7501 ttcaaagcag atcatttcga acagtttttt actgtcagtt tcaggcacgc cccactcatt
     7561 atcatggtag gcaatataaa gcgggccctg actcacccag ccgcaacgtt ccatactttc
     7621 cctcgctatg tacccgatcg taaaaatttc agccgctttt cttgacgcat cggtgaaaaa
     7681 gtcaatattt aatacagggc tactatgccc gatatccttc atacaatgac aataatattg
     7741 ccgaattcgg ctcttccctg gagtcgtgtt aatgatcata aaaaaaagcg gtggtcgctg
     7801 gcagctaagc ctgctggcga gcgtggtaat cagtgccttt tttctcaaca cagcttacgc
     7861 ctggcaacaa gaatatatcg ttgatacgca acccggacat tccacagagc gttacacctg
     7921 ggatagtgat catcaacctg attacaacga tattttgtcg caacgtattc aaagtagcca
     7981 aagggcgctg ggactggaag tcaatctggc ggaagaaact cctgtggatg tgaccagcag
     8041 tatgagtatg ggctggaatt ttcctttata tgaacaggtt acaaccggcc cggtcgcggc
     8101 attacattac gatggcacaa ccacctcgat gtataacgag tttggcgaca gtactaccac
     8161 gctgaccgat ccgttatggc atgccagcgt gagtagctta ggctggcgtg ttgactcccg
     8221 gcttggcgat ctccgaccct gggcgcaaat cagctataac cagcaatttg gcgagaatat
     8281 ctggaaggcg caatcaggcc tgagccggat gacggcgaca aaccagaacg gcaactggct
     8341 ggatgtcacc gtaggcgctg atatgttgct caatcaaaat attgccgcct atgctgcgct
     8401 aactcaggca gaaaatacca ctaataatag cgactatctg tatacgatgg gggttagcgc
     8461 cagattttaa cgtaacagtc acaattgaaa ccattgaata acaacagttg tggcgatagt
     8521 gggtgctaac ttaccaaata ataaatttgg tgaataattg tcgcgtcatt cattcctgaa
     8581 ctaaggcatt tcattccgtt ctgatggcat ttcatgccgt ttttccccaa gcataaaatg
     8641 cacttcgtta tggttgtcgg cagagatttt tcctttttat tactgcagga atactgccat
     8701 gacaccttca aattatcagc gtacccgctg gctgacactc atcggtacta tcattaccca
     8761 gtttgcgctg gggtcggttt atacctggag cctgtttaat ggcgcgcttt ctgccaagct
     8821 ggatgcgccg gtaagccagg tcgctttctc tttcggcttg ttaagtctgg ggctggcaat
     8881 ttcgtcttct gttgcgggca aattgcagga acgttttggc gttaaacgcg tcaccgtggc
     8941 ttccggcatt ttgctgggat taggcttctt cctgaccgcg cattccaaca acctgatgat
     9001 gctgtggtta agcgccggtg tgctggtcgg tctggcggat ggcgcgggtt acctgctgac
     9061 gctctctaac tgcgtgaagt ggttcccgga gcgtaaaggt ctgatctccg cgttcgctat
     9121 cggttcttat ggtctgggta gcctgggttt caaatttatc gacacgcagc tgctcgaaac
     9181 ggtgggtctg gaaaaaacct ttgtgatttg gggagcgatt gcgctggtga tgattgtttt
     9241 cggcgcaacg ttaatgaaag acgcaccaaa acaggaagtg aaaaccagca atggtgtggt
     9301 ggagaaagat tacacgctgg cagagtcgat gcgtaaaccg cagtactgga tgttagcggt
     9361 aatgttcctg actgcctgca tgagcggcct gtacgtgatt ggggtagcga aagatatcgc
     9421 ccaaagtctg gcacaccttg atgtggtttc cgcagccaat gcagtaacgg ttatttccat
     9481 cgctaacctt tcaggtcgtc tggtgctggg cattctgtct gacaaaattg cccgtatccg
     9541 tgttatcacc attggtcagg tgatttcgct ggtgggtatg gcggccctgc tgtttgcacc
     9601 attaaatgca gtgacgttct ttgcagcgat tgcctgcgtg gcatttaact ttggcggcac
     9661 tattaccgtc tttccatcac tggtcagtga gttcttcggc ctcaataacc tggcgaaaaa
     9721 ctacggtgtg atttacctcg gtttcggtat cggtagcatt tttggttcga ttatcgcctc
     9781 actgtttggc ggtttctatg tgaccttcta cgtgattttc gccctgctga ttctgtcatt
     9841 ggcgctttct acgacgattc gtcagccaga gcagaaaatg ttgcgtgagg cgcatggctc
     9901 cctttaatgc tcagccgcga gctactgggc ttcagcttcc gtgtgcatca aacgcatcaa
     9961 cgaaatgtat aaacaatgac tcgtaaaatt tgggccagtt aactggccct ttttactgct
    10021 ttttgtaacg ctaaagtctc ttttcaaact tgcatttttg taaatttgtg cttcatgcac
    10081 actctttccc cacacttttt cgctttgctg tggtctactt attcgcgcgt gtagatttta
    10141 cttatctgac tacctccgca cttttcccct gccgggcctg aaaagccgct aagcagggtg
    10201 ttatcacctg tttgtccagg gtttgtttgc atgagataca tcaaatcgat tacacagcag
    10261 aagctgagct ttttgcttgc aatctatatt ggccttttta tgaatggcgc ggttttttat
    10321 cgccgcttcg gcagctatgc gcacgatttt accgtctgga aaggcgtttc tgctgttgtt
    10381 gaactggccg ccaccgtact ggtgaccttc tttttactac gtcttctatc gctgtttggc
    10441 cgccgcagct ggcgtattct ggcgtcgctg gtggtgctct tttccgcagg tgccagctat
    10501 tacatgacct tccttaatgt ggtcattggt tatggcatca tcgcttccgt catgaccacc
    10561 gatatcgacc tgtcaaaaga agttgttggt ctgaacttta ttctctggtt aatcgccgtt
    10621 agtgcattgc ctcttatcct tatctggaat aaccgctgtc gctacacctt gctccgacaa
    10681 ctgcgaaccc cagggcagcg tattcgcagc ctggtggtcg tcgtactggc gggtattatg
    10741 gtttgggcac cgattcgttt gctggatatc cagcagaaga aagtggagag ggcgaccggc
    10801 gttgatttgc cgagttatgg cggtgtcgta gcgaactctt atctgccatc aaactggctt
    10861 tctgcgttgg ggctgtatgc ctgggcgcgg gtcgatgaat cttccgataa taattcattg
    10921 cttaatccgg cgaagaagtt tacataccag gctccacaaa acgttgatga tacttatgtc
    10981 gtgtttatca tcggtgaaac cacgcgttgg gaccatatgg gtattttcgg ctatgagcgt
    11041 aataccacgc cgaaactggc ccaggagaaa aatctggcgg cgttccgtgg ttactcctgt
    11101 gataccgcaa ccaaactctc actgcgttgc atgtttgtac gtcagggggg cgcggaagat
    11161 aatccgcagc gcacattaaa agaacagaac attttcgcgg ttctgaagca gttaggattc
    11221 agttctgacc tctacgctat gcagagcgaa atgtggttct acagcaacac gatggcagac
    11281 aacattgctt atcgtgagca gattggtgcg gagccacgta atcgtggcaa gccggtagat
    11341 gatatgttgc tggtagacga aatgcagcaa tcgctagggc gcaacccgga tggtaagcat
    11401 ctgatcattc tgcataccaa aggttcgcat tttaactaca cccagcgtta tccgcgtagc
    11461 ttcgcgcagt ggaagccgga atgtattggt gttgatagcg gctgtaccaa agcgcagatg
    11521 atcaactcct atgacaactc ggtgacctat gtggatcact ttatctccag cgtgattgat
    11581 caggttcgcg ataagaaagc gattgtgttc tacgcagctg accacggtga gtcaattaat
    11641 gaacgcgagc acctgcacgg cacgccgcgt gaactggcac cgccggagca gttccgcgta
    11701 ccgatgatgg tctggatgtc agataaatat ctggaaaatc cggccaatgc gcaggcgttt
    11761 gcgcagctga aaaaagaagc cgacatgaaa gtgccacgcc gtcacgtaga gctgtacgac
    11821 accatcatgg gttgtcttgg ctatacttca ccggatggtg gaattaacga aaacaacaac
    11881 tggtgtcaca tcccgcagac aaaagtggca gcggctaact aagcggcctg ctgactttct
    11941 cgccgatcaa aaggcatttt gctattaagg gattgacgag ggcgtatctg cgcagtaaga
    12001 tgcgccccgc attcggtgat tggcgcagcc tggtagcgca cttcgttcgg gacgaagggg
    12061 tcggaggttc gaatcctcta tcaccgacca aattcgaaaa gcctgctcaa cgagcaggtt
    12121 tttttgcatt tgtagctcat gaggatgaga acctccgggg gcagggaggc tcgactcgac
    12181 gtaggcctga taagacgcgc cagcgtcgca tcaggcaacg gcaccgcact tattgtcgga
    12241 tgcggcgcga ccgccttatt cgaccaacac accacacatt ttcctgccaa ctttgcgccc
    12301 catatctcac tatccattaa cggatttgtg acagaaacca ttgtcttttt ttatatttgc
    12361 ttaaatcttt tttgcgttac ttatggctgg cgctggcatt ttccgctgtg cgttttagcg
    12421 ttcatggtat taatcgctca gatatccacc ttttcctcag atttttttca ttaaatgcat
    12481 aaaacttaat caccaaatgt tgctaaatat taagctgatt gttctggtta tgttggagta
    12541 gcacaaattt ctctgctgca aatagctgtt tgaagttttt ttaatctttg tttgtggatt
    12601 ctcaccgttg gtgtaaatcc cggttggctg tattgacgtt ttcacattct gttgacagat
    12661 tgtaggtcac gaggggcatt ttatggagga tccgcactgt tacactgatg ttaattagta
    12721 cggcatcccc acctcataac gttgacccga ccgggcaaaa aacaaaaaag gtcaggcagc
    12781 gacaacccac tgcaaagggt taaaacaaca aacatcacaa ttggagcaga ataatgcgta
    12841 tttccttgaa aaagtcaggg atgctgaagc ttggtctcag cctggtggct atgaccgtcg
    12901 cagcaagtgt tcaggctaaa actctggttt attgctcaga aggatctccg gaagggttta
    12961 acccgcagct gtttacctcc ggcaccacct atgacgcctc ttccgtcccg ctttataacc
    13021 gcctggttga atttaaaatc ggcaccaccg aagtgatccc aggcctcgct gaaaagtggg
    13081 aagtcagcga agacggtaaa acctatacct tccatctgcg taaaggtgtg aagtggcacg
    13141 acaataaaga attcaaaccg acgcgcgaaa tgaacgccga tgacgtggtg ttctcgttcg
    13201 atcgtcagaa aaacgcgcaa aacccgtacc ataaagtttc tggcggcagc tacgaatact
    13261 tcgaaggtat gggcttgccg gagctgatca gcgaagtgaa aaaggtggac gataacaccg
    13321 ttcagtttgt gctgactcga ccggaagcgc cgttcctcgc tgacctggca atggacttcg
    13381 cctctattct gtcaaaagaa tatgctgacg cgatgatgaa agccggtaca ccggaaaaac
    13441 tggacctcaa cccaatcgga accggtccgt tccagttaca gcagtaccaa aaagattccc
    13501 gtatccgcta caaagcgttt gatggctact ggggcaccaa accgcagatc gatacgctgg
    13561 tcttctctat tacccctgat gcttccgtgc gttacgcgaa attgcagaag aatgaatgcc
    13621 aggtgatgcc gtacccgaac ccggcagaca tcgctcgcat gaagcaggat aaatccatca
    13681 acctgatgga aatgccgggg ctgaacgtcg gctacctctc gtataacgtg cagaaaaaac
    13741 cgctggatga cgtgaaagtt cgccaagcac taacctacgc ggtgaacaaa gacgcgatca
    13801 tcaaagcggt ttatcagggc gcgggtgtat cagcgaaaaa cctgatcccg ccaaccatgt
    13861 ggggctataa cgacgacgtt caggattaca cctacgatcc tgaaaaagcg aaagccttgc
    13921 tgaaagaagc gggtctggaa aaaggtttct ccatcgacct gtgggcgatg ccggtacaac
    13981 gtccgtataa cccgaacgct cgccgcatgg cggagatgat tcaggcagac tgggcgaaag
    14041 tgggcgtgca ggccaaaatc gtcacctacg aatggggtga gtacctcaag cgtgcgaaag
    14101 atggcgaaca tcagacggta atgatgggct ggactggcga taacggggac ccggataact
    14161 tcttcgccac cctgttcagc tgcgccgcct ctgaacaagg ttctaactac tcaaaatggt
    14221 gctacaaacc gtttgaagat ctgattcaac cggcgcgtgc taccgacgac cataacaaac
    14281 gcgttgaact gtacaagcaa gcgcaggtgg tgatgcacga tcaggctccg gcactgatca
    14341 tcgctcactc caccgtgttt gaaccggtac gcaaagaagt caaaggctat gtggttgatc
    14401 cattaggcaa acatcacttc gaaaacgtct ctatcgaata attaaaagcc atacaagact
    14461 gatggcaaag gcaaaactgc ctgatgtgct ccgcttatca ggcctacgaa aattctgcaa
    14521 tgtattgaat ttgcacgatt ttgtaggccg gataaggcgt taacgccgca tccggcataa
    14581 acaaagcgca ctttgtcaac aatctgtata cccggtggcg ctgtgcctct gcgtgcagcg
    14641 ccatccggca gcaatacttc attctctgtc cgacaagggc gggaattgat ttgtgagcaa
    14701 tacagacacg cagttccagg ctgcgagtca ctatagagaa tccgggttat gttgcagttt
    14761 attctccgac gtttgggact cgtcatcccc acgtttatcg gtattaccct tctcacattt
    14821 gcctttgtcc acatgatccc gggcgatccg gtgatgatca tggcaggcga acgtgggatc
    14881 tcccctgagc gtcacgtgca gctgctggct gaactcggct tagataaacc gatgtggcag
    14941 cagtatctcc attacatttg gggcgtaatg cacggcgatc taggcatttc aatgaaaagc
    15001 cgaattccag tatgggaaga gttcgtgccg cgctttcagg ccacgctgga acttggcgtc
    15061 tgcgcgatga tttttgctac ggcagtcggt attccggttg gtgtgctggc tgcggttaaa
    15121 cgcggttcca ttttcgatca cactgcagtt ggcctggcgc tgaccggcta ctcgatgccg
    15181 atcttctggt ggggcatgat gctgatcatg ctggtttcgg tgcactggaa cctgacgccc
    15241 gtctccggtc gcgtgagcga tatggtgttc ctcgatgact ccaatccgtt aaccggtttt
    15301 atgctaatcg acaccgccat ctggggtgaa gacggcaact ttatcgatgc cgtcgcccat
    15361 atgatcttgc ccgccattgt gctgggtact attccgctag cggtcattgt gcgtatgaca
    15421 cgctcctcga tgctggaagt gctgggcgag gattacatcc gcaccgcgcg cgccaaaggg
    15481 ctaacccgca tgcgggtgat tatcgtccat gcgctgcgta acgcgatgct gccggtagtg
    15541 accgttatcg gcctccaggt gggaacattg ctggcggggg cgattctgac cgaaaccatc
    15601 ttctcgtggc ccggtctggg gcgctggttg attgacgcac tgcaacgccg cgactatccg
    15661 gtagtgcagg gcggcgtatt gctggtggcg acgatgatta tcctcgtcaa cttgctggtc
    15721 gatctgctgt acggcgtggt gaacccgcgt attcgtcata agaagtaagg ggacatcatg
    15781 tcacaggtta ctgaaaataa agtgattagc gcaccggtgc cgatgacccc gttacaggag
    15841 ttctggcact attttaaacg caacaaaggc gcggtcgtcg ggctggttta cgtcgccatc
    15901 gtgctgttca tcgcgatctt tgccaactgg attgcgcctt ataacccggc ggatcagttc
    15961 cgcgatgcgc tgctcgcccc gccagcctgg caggaaggcg gcagtatggc acacttgctg
    16021 ggcaccgatg acgtcggtcg tgatgtgctg tcgcggctga tgtacggcgc gcgtttatcg
    16081 ctgctggttg gctgtctggt ggtggtgttg tcgctgatta tgggcgttat tctcggcctg
    16141 atcgccggtt acttcggcgg cctggttgat aacattatca tgcgcgtggt cgatatcatg
    16201 ctggcgctgc caagtctgct gttggcactg gtgctggtgg cgattttcgg cccgtcgatt
    16261 ggtaacgccg cgctggcgct gaccttcgtt gccttgccgc actatgtgcg cttaacccgc
    16321 gccgccgtgc tggtggaggt gaatcgcgat tacgtcaccg cctctcgcgt ggcaggtgcc
    16381 ggggcgatgc gccagatgtt tattaacatc ttcccgaact gccttgcgcc gctgattgtt
    16441 caggcgtcgc tcggtttctc taacgccatt ctcgatatgg ctgctcttgg tttcctcggc
    16501 atgggggcac agccgccaac gcctgagtgg ggcaccatgc tctccgacgt gttgcagttc
    16561 gcgcaaagcg cctggtgggt cgtgaccttc cctggtctgg cgatcctgtt gacggtgctg
    16621 gcatttaacc tgatgggtga cggtctgcgt gacgcgctcg atcccaaact aaagcagtaa
    16681 gaggttcgag atggcgttat taaatgtaga taaattatcg gtgcatttcg gcgacgaaag
    16741 cgcaccgttc cgcgccgtag accgcatcag ctacagcgta aaacagggcg aagtggtcgg
    16801 gattgtgggt gagtccggct ccggtaagtc ggtcagttca ctggcgatta tggggctgat
    16861 tgattatccg ggccgcgtaa tggcagaaaa actggagttt aacggccagg atttgcagcg
    16921 tatctcagaa aaagagcgcc gcaacctggt gggtgccgaa gtggcgatga tcttccagga
    16981 cccgatgacc agccttaacc cgtgctacac cgtgggtttc cagattatgg aagcgattaa
    17041 agtgcatcag ggcggcaaca aaagtacccg ccgtcagcgg gcgattgacc tgctgaatca
    17101 ggtcggtatt cccgatccgg cttcgcgtct ggatgtttac ccgcatcagc tttccggcgg
    17161 catgagccag cgcgtgatga tcgccatggc gattgcctgt cggccaaaac tgctgattgc
    17221 cgatgaaccg accaccgcgc tggacgtgac cattcaggcg caaatcatcg aactactgct
    17281 ggagctacag cagaaagaga acatggcgct ggtgttaatt acccatgacc tggcgctggt
    17341 ggcggaagct gcgcataaaa tcattgtgat gtatgcaggt caggtagtag aaacgggcga
    17401 tgcacacgcc atcttccatg cgccgcgtca cccgtatact caggcattgc tgcgtgcgct
    17461 gccagaattt gctcaggaca aagaacgtct ggcgtcgttg ccaggtgttg ttcccggcaa
    17521 gtatgaccgc ccgaacggct gcctgcttaa cccgcgctgc ccctatgcca ctgacagatg
    17581 tcgcgctgaa gaaccggcgc tgaatatgct cgctgatggg cgtcagtcca aatgccatta
    17641 cccacttgat gatgccggga ggcccacact atgagtacgc aagaggccac ctcgcaacaa
    17701 ccgctgttgc aggctatcga cctgaaaaaa cattatccgg tgaagaaagg catgttcgtg
    17761 ccggaacgtc tggtgaaggc gctggacggc gtttcgttta accttgaacg tggcaaaacg
    17821 ctggcagtag tgggcgaatc tggctgcggt aaatcgaccc tcggtcggtt gctgacgatg
    17881 atcgaaacgc ccaccggcgg cgagctgtat tatcaggggc aggatctgct caagcacgat
    17941 ccgcaggcgc agaagctgcg tcggcagaaa atccagatcg tcttccagaa tccctacggt
    18001 tcgctgaatc cgcgtaagaa agttgggcaa attctcgaag agccgctgct tattaacacc
    18061 agcttaagca aagaacagcg tcgggaaaaa gccctgtcga tgatggcaaa agtcggcctg
    18121 aaaaccgagc actatgaccg ctatccgcac atgttttccg gcggacaacg tcagcgtatc
    18181 gctattgccc gtggtctgat gctcgacccg gatgtggtga ttgccgatga gccggtttct
    18241 gcgctggacg tctcagtgcg tgcgcaggtg ctgaatctga tgatggattt gcagcaggag
    18301 ttggggcttt cttatgtctt tatctcccac gacctgtcgg ttgtggagca cattgctgat
    18361 gaagtgatgg tgatgtacct aggccgctgc gtggagaaag gaacgaagga ccaaatcttc
    18421 aataacccgc gccatccgta cacccaggcg ctgctctccg cgacgccacg cctgaacccg
    18481 gacgatcgtc gtgaacgcat caaactcacc ggtgaactgc caagcccgct gaatcctccg
    18541 ccgggttgcg ccttcaacgc ccgctgtcgt cggcgcttcg gcccctgcac ccagttgcag
    18601 ccgcagctaa aagactacgg aggtcaactg gtagcttgtt ttgctgttga tcaggatgaa
    18661 aatccgcagc gttaactcac aaaaccgggg ccatgcgctc cggtttactc aatcagcttc
    18721 aacagtggcg acaagcatag caaaatgccg taaagcagaa tcagccaggc tttaaatccg
    18781 cgaagttttt ccagttgtgc gactttatag atcaggaaaa acggaatgag gcacgacaca
    18841 atgccatata acgggcttcc caactgaaag aacaccagca ctgatacacg aaacgaaacc
    18901 caaatcgtca acgtaatgac gataaaagcg cagatcgcca gagtcagcat gcgtgagtta
    18961 attttcttgg tatcaataat tcggcttaac agattgagaa taatgccttt aatagcctcg
    19021 tggaaaccga gataaatgcc aaagaatgcg gtcagtacgg caaagatatt cagcaccgta
    19081 gaggtgatat gaatgatatg tccagggatc acctgcgcgg ccagtgccag tgccgagata
    19141 ttttgttcaa aggcagaaac ggcttcttcg tggctaattg agaaggtaaa cgaaaaggca
    19201 aaaaacagga tcaccgcgat gagcgtgata taactaattc ggtgggtacg cagcgcgagc
    19261 cgggttgcca gtactttatc tgcttcccgt ttacgatagg caatattcat tgggttaagt
    19321 acctgaataa ataccgcaga aaagaagcaa aatggaatgg taagcaggac atcgcggaaa
    19381 aagacggagg cttgcgggaa ggcggttata ttggcgaaat tccagtgcgg gatcatcgca
    19441 aaaccgaaca cgacaataat ccctactttg accaccacca ttggcccaga aatcttaaac
    19501 agtaatcgtt caccaccaga cgcaatcgct atcagtacgg cgaaaatagc gactttataa
    19561 agtagagatt gtgaaagatc ggcatcggtt aaaccgaagg ttttcaggta cgaggcgctg
    19621 tcgaaaacca cggagagaga gtagataaaa atcccgtgga taatcatcaa aaagtagata
    19681 accccgagga aaattcccca gttcttcccc aggtaatgac tgataatatc ggtgtagtca
    19741 ttacaggaat cgctttcaga aagggttttt aaataaatgt cctgcactac ccaggtgaca
    19801 ggataagcaa tgatcgctgc ggtaataaat acccaaattc ccttcaagcc aatttgtact
    19861 ggcatcagca cggttccggc accaatcgcc atgcctatgc ataataaaac ccagccgaag
    19921 tcgtagcgtg taaacggcaa tttctgattg tgtttcgata gtgtgttgtg ctgcataaag
    19981 tcctgtccag aaagaagtcg gcctcgcaaa aatgaataaa cccagtttct cttgcgcaac
    20041 aggtggagaa gtcgaatacg cagtatgtaa aaaatgaggc aacagattca ctggggaggt
    20101 cgcgaggagg cgcaattcta caaaattgcg catttcacta ccagtctgga gtcagcccgg
    20161 atatgaactg caactattgc aaataaagaa attaagctgg aaggcatcgc gcaatgagga
    20221 agattgccgg gtgctgacag taaaaagcaa tcgtagaatc agcgtcaagc cggaaggatt
    20281 ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg cattggttgc tggagagaga
    20341 aaacccccgc acgttgcagg tatgtacctg acaacataac gggggctaat cttgaaccta
    20401 gaccactcaa gaatagccgc gaaacgttgt cattacaaca caggcggcta tatgacgctc
    20461 gcagagctgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggcattctt
    20521 gccagtatga tcgtgaactg gctgaacaag cggaagtagc gtgtcatgtg ggcgtcaggc
    20581 tgccgtaatg gcaatttgcg cccggaccag gccgcagggg tgaaactctg cggccttttt
    20641 cgtaagaaaa atgcaacggt tgcaagcgga taatattctg cggaatgcgc agcacagagt
    20701 gaagatgaga cggattccgg aacagaaaaa cagtcgtaga atcagcgtca agccggaagg
    20761 attccggtga ggcgcaatgt tgcgggggct taatccctgg tggcattggt tgctggagag
    20821 agaaaacccc cgcacgttgc aggtatgcac ctgacaacac cacgggggcc attcttgcat
    20881 ccaaacaata caagaatagc cgcgaaacgt tgtcattaca atacaggcgg ctatatgacg
    20941 ttcgcagaac tgggcatggc cttctggcat gatttagcgg ctccggtcat tgctggtatt
    21001 cttgccagta tgatcgtgaa ctggctgaac aagcggaagt aacgtgccat gcgggcgtca
    21061 ggctgccgta atggcaattt gcgcccggac caggccgcag gggtgaaact ctgcggcctt
    21121 tttcgtaaga aaaatgcaac ggttgcaagc ggataatatt ctgcggaatg cgcagcacag
    21181 agtgaagacg tgacggattc cggaacagaa aaacagtcgt agaatcagcg tcaagccgga
    21241 agggttccgg tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt ggttgctgga
    21301 gagagaaaac ccccgcacgt tgcaggtatg cacctgacaa catcacgggg gccattcttg
    21361 aacctgaaca actcaagaat agccgcgaaa cgttgtcatt acaacacagg cggctatatg
    21421 acgctcgcag aactgggcat ggccttctgg catgatttag cggctccggt cattgctggc
    21481 attcttgcca gtatgatcgt gaactggctg aacaagcgga agtaacgtgt catgcgggcg
    21541 tcaggctgcc gtaatggcaa tttgcgcccg gaccaggccg caggggtgaa actctgcggc
    21601 ctttttcgta agaaaaatgc aacgattgca cgcggataat attctgcgga atgcgcagca
    21661 cagagtgaag acgtgacgga ttccggaaca gaaaaacagt cgtagaatca gcggcaagcc
    21721 ggaaaggttc cggtgaggcg caatgttgcg ggggctttat ccctggtggc attggttgct
    21781 ggagagagaa aacccccgca cgttgcaggt atgcacctga caacatcacg ggggctaatc
    21841 ttgaacccaa acaactcaag aatagccgcg aaacgttggc attacaacac aggcggctat
    21901 atgacgctcg cagaactggg catggccttc tggcatgatt tagcggctcc ggtcattgct
    21961 ggcattcttg ccagtatgat cgtgaactgg ctgaataagc ggaagtaacg tgtcatgcgg
    22021 gcgtcaggct gccgtaatgg caatttgcgc ccggatcagg ccgcagaggt taaactctgc
    22081 ggcctttttc gtgcttactg cgggtaaggc acccagtcgc cgccgttcag gcgaacgtac
    22141 ggtttatctt ggtattgaat aactactgca tttgagttct cggagaccgg tgctgtttgt
    22201 ggcaacccac tggtgagttt tttccagtca acattgtctt cggtgaaaat cttgccatca
    22261 agaacgcgaa ccaccagatc ggagatagcc aggaagctgc tcggttggtc aatgacaatc
    22321 ggtgccccct gatgtggtgc cttcatgccg aagaatttca ccccaacggg gacgtctgtg
    22381 atagacgggc tagggatatc acgtaggcca gatacctgca ttctgtcgcc cttcagcgcg
    22441 ccgccgtgtt ccggcaccac gaccaccatc actttacgac ccgatttctc cagttcagta
    22501 aagaaggcgt ccagttcatc aaagaatttc tgcgcccgcg ctttgtaatc cgctgttttg
    22561 ctgacccccg gataatggtt gccgtcatgc agtggaagcg tgttgtagaa cgtggcacta
    22621 cggctgtttt tatctttttc ggtaacgtcc agccagcggt taagcacggc ggtatcgtca
    22681 taaaccggcg aaccatcaaa gcccagcaaa ataaccggca gatttgtttg atccatcaat
    22741 tcagtctgca tgccgccatt ttcgcgaact tctttcaaaa aaccgccgaa ctggccgtta
    22801 tgtcccatca tcaggtgctg ggtaaagccc agtttcgaaa ggttatcaaa cagatagcag
    22861 tcgttatttg ccggttgata cagattagtg tgcgaagtct gcccgcagct ggcgcgtagt
    22921 aaacggatcg ccgccgggcc actgtaggag gtggcggagt taaagttctt gaaatcaata
    22981 tcgaaatgcg accacagtgg atgcgacatc aacccggcgg cttctatatc cgaccaggaa
    23041 agcgaacaga tgttaatcac cagtagttca aatggctgag catcagcggg cagcgaagac
    23101 gggaaggtcg atttacgttt cgcctccgcg ttatagaaat tattcagcca ggcgttaagg
    23161 ttcgccgttg ttggcggtgc agtttgtgcg ggcatatcac ccactaccgg tgcgccaccc
    23221 gtcgccgcaa cggttgccgc tgcgttacca cccgtcgttg ttacagtggt cgtcggttgt
    23281 ccggctggcc acaaggagaa acttggtccc gccagggtaa gtacgttcag ccatagcagt
    23341 atggcaacca caaaaacggt aatgcgaatc cattgtgaca ggaataacca ggccactaat
    23401 aaaacaaaaa tggccccaat catctgccag ttaataaagc gtgtgacaag gtcgattaaa
    23461 taatcggtac tgaaccccgc cacctgcgaa ccctggctca ttatgctttc cgggccaggc
    23521 aaccagatgt catgccagaa caaagcaaag ccgatcggca gggcaatcca gtggcgcaag
    23581 cgatgcaggc tgtaacgcgg aatgggcatc agcagaaacg cggcaaacac caaattgagg
    23641 agcgggtgga agttaagata tcccgcccac aacaggccaa acttaaccag aaaatagaag
    23701 ttccagccgg aaaggccgcg ccagtattgc cagagggaag aaggcatggc ggtattttgc
    23761 gtaaattgag tcattttttg gttgccctgg ctttttccgt gcggcgtaac gtcccggccg
    23821 gtttaacata acgaggttta gcaaagaaca aacgtaaggt gtcgcgaatg cgtcgcaaag
    23881 agtgccgcgc cagatagccc agcgggaaaa atatcagtgc gcaaacgata ataatttcaa
    23941 tgatatcgct gatggtcatc atgatgagcg ctccacagca tcatctaaca gtcgcatggg
    24001 ttctggtatt cgtcgccagt ggcgaccatc gtgctcggca ttgatgaccg gtttagaact
    24061 ttgcgttaaa ggcagcggca ttccccattg ttctggggca agcaggcgca tctgcaccag
    24121 ctcggcactg atttgatcat cttcaaacca gaccatacgg tttgagaaaa tgtcgccagt
    24181 aggcaatggg aaaatatgat tcaacgcggt atccagatcg ttaatccgac agaatgagag
    24241 aaacagcacc agccgattac cgccaatggt catgatatcg ccggtacggt tagggcgaca
    24301 tagcgtcagg gcttgctcaa cgcggatacc cggtaccgga cgtagggcaa ccagaacgcc
    24361 tttaccgtgg gcaggcaaga gagggttatt catcatgttg ttgacggcat tacagaacac
    24421 atcccacttc tggaaaccac gcagtttgag cggctgggtc attgacagca aggtagtgat
    24481 atcttccggt acatagcgac taaacttctg cccttgcacg ctttcgatca tcgtcagaca
    24541 acgggagagt ggcgcattcc acggaataac catatttgca ccgcaggcca ataacaaacg
    24601 ttcatcggtg gcgcgcaggc tagcggtatt ttcccgcacg aggattttca tcgcactacc
    24661 gcgctggcga cgcagggtat gaatgctgcg ggccagtggc tcgatttgcg cattttgctg
    24721 taaagaaaag accaccgtcg ccgcctgagc ggtacgggct tcattgaaca ggacttcgtt
    24781 attgttgaac agttgccagt gttccgatag cggcggcgca ccttccagta cagcaacatt
    24841 actcagaatg cgtttttcgt cgctgcgtgg ttggatctcc gcctcttcgc tttgaactaa
    24901 tgtccagata ccattttgtt gctgaacgct aagctgctga cgggcgctga cccctttttc
    24961 gttgcaccag aaggcaatat ccagcaaatg ttggtcaccc tgaaaacgca aactggcaag
    25021 accaaaaagt gaacggtatt cctcaagcaa caatgaaaat tgtttatcgt tattatttcc
    25081 gggattaatt accaaaagcg aacaatggtt taacctgctc catttattca ttttatccaa
    25141 ccatgagcga agccgctcgg caggaatatt ttgccatgcg ttatttgcgc aaacaagaat
    25201 aaaaaggtaa ttatggggat caatagaaca ctgcaaatca cgggccaaat agtatagacc
    25261 tttttcatga tttagcattg aaaataattt tattttttcc ggcccttgag aatcatctaa
    25321 ttgaaagatt ttcgccggat cgctgtccat gctaatgacc gcgacgtgtg cggtttcagc
    25381 ctgggatgca attgtttgat tcgccagact gatagcatct tcatggcgat cgacgttaaa
    25441 ccaccagacg ccgcctgctg gcatatgtcg cagctcatcc cataatgatg agataccgat
    25501 agagaataca gggtccacaa tgtccctcat gtcaccattt tgtctgatta tcagtttact
    25561 agcgaaagct aagaaataaa cctaccattg aaattaaaac ttatcagatt tagcatgtga
    25621 atcaatttag aaggtcacaa gacgtgtgat tttagtcttt tcgtttttaa ctttttctgt
    25681 tgcgtaacgg aaagtcaaaa agtgagcaaa ttcccgtctc gccgtaaaca ataaaccggc
    25741 gaaatgctca cggttagaat tgtctcatga atggtacggt tatttcatag ggatcaagca
    25801 aaatgaataa caatgaacca gatactctgc ctgatcccgc gataggctat atcttccaga
    25861 atgatattgt ggcgttaaag caggcatttt cactgcctga tattgattat gccgatattt
    25921 cccaacgcga acagttggcc gcggcattaa aacgctggcc gttgctggca gagtttgcgc
    25981 aacaaaagta ggggattggt gaatggccgt actgggattg cagggggtgc ggggaggcgt
    26041 ggggacaaca accatcaccg ccgcattagc ctggtcatta caaatgttgg gagaaaatgt
    26101 cctggtggtc gatgcctgcc cggacaactt gttgcgcctg tcatttaatg ttgattttac
    26161 ccaccgtcag ggctgggcca gagcgatgct ggatggccag gactggcgtg acgctgggtt
    26221 gcgctacacc tcgcagctcg atttgctgcc ttttggtcag ttatccattg aagaacaaga
    26281 aaatccacag cactggcaaa cccggctgag cgatatttgc tccggcttac agcaactaaa
    26341 agccagcggg cgttaccagt ggattttaat cgacttaccg cgtgatgcct cgcagataac
    26401 ccaccagctg ctgagtttgt gcgagcactc gctggcaatc gtcaatgtgg atgccaactg
    26461 ccatatccga ctgcatcagc aagcgctgcc ggatggcgca catattttga ttaatgactt
    26521 ccgtattggc agtcaggttc aggacgatat ttaccagctt tggttgcaaa gccagcgccg
    26581 attactgccg atgctcattc atcgtgatga agcgatggct gaatgcctgg cggctaagca
    26641 accagtaggt gaatatcgca gtgatgcgct ggcggctgaa gagatactga cgctggcgaa
    26701 ctggtgcctg ttgaactact ccgggctgaa aacgccagtc gggagtgcat catgagtatc
    26761 ctgacccggt ggttgcttat cccgccggtc aacgcgcggc ttatcgggcg ttatcgcgat
    26821 tatcgtcgtc acggtgcgtc ggctttcagc gcgacgctcg gctgtttctg gatgatcctg
    26881 gcctggattt ttattccgct ggagcccccg cgctggcagc gtattcgcgc agaacataaa
    26941 aacctgtata cgcatatcaa cgcctcgcgt ccgcgtccgc tggacccggt ccgttatctc
    27001 attcaaacat gctggttatt gatcggtgca tcgcgcaaag aaacgccgaa accgcgcagc
    27061 gggcattttc aggtctgcaa aatattcgtg gacgttacca tcaatggatg aacgagctgc
    27121 ctgagcgcgt tagccataaa acacagcatc tggatgaaaa aaaagagctc ggtcatttga
    27181 gtgccggggc gcggcggttg atcctcggta tcatcgtcac cttctcgctg attctggcgt
    27241 taatctgcgt tactcagccg tttaacccgc tggcgcagtt tatcttcctg atgctgctgt
    27301 ggggggtagc gctgatcgta cggcggatgc cggggcgctt ctcggcgcta atgttgattg
    27361 tgctgtcgct gaccgtttct tgccgttata tctggtggcg ttacacctct acgctgaact
    27421 gggacgatcc ggtcagcctg gtgtgcgggc ttattctgct cttcgctgaa acgtacgcgt
    27481 ggattgtgct ggtgctcggc tacttccagg tagtatggcc gctgaatcgt cagccggtgc
    27541 cattgccgaa ggatatgtcg ctgtggccgt cagtggatat ctttgtcccg acttacaacg
    27601 aagatctcaa tgtggtgaaa aataccattt acgcctcgct gggtatcgac tggccgaaag
    27661 acaaactgaa catctggatc ctcgatgacg gcggcaggga agagtttcgc cagtttgcgc
    27721 aaaacgtggg ggtgaaatat atcgcccgca ccactcatga acatgcgaaa gcgggcaaca
    27781 tcaacaatgc gctgaaatat gccaaaggcg agttcgtgtc gattttcgac tgcgaccacg
    27841 taccaacgcg atcgttcctg caaatgacca tgggctggtt cctgaaagag aaacagctgg
    27901 cgatgatgca gactccacac cacttcttct cgccggaccc gtttgaacgc aacctgggac
    27961 gttttcgtaa aacgccgaac gaaggcacgc tgttctatgg tctggtgcag gatggcaacg
    28021 atatgtggga cgccactttc ttctgcggtt cctgtgcggt gattcgtcgt aagccgctgg
    28081 atgaaattgg cggcattgct gtcgaaaccg tgactgaaga tgcgcatact tctctgcgtt
    28141 tgcaccgtcg tggctatacc tccgcgtata tgcgtattcc gcaggcggcg gggctggcga
    28201 ccgaaagtct gtcggcgcat atcggtcagc gtattcgctg ggcgcgcggg atggtataaa
    28261 tcttccgtct cgataacccg ctcaccggta aaggactgaa gtttgctcag cggctgtgct
    28321 acgtcaacgc catgttccac ttcttgtcgg gcattccacg gctgatcttc ctgactgcgc
    28381 cgctggcgtt cctgctgctt catgcctaca tcatctatgc gccagcgttg atgatcgccc
    28441 tattcgtgct gccgcatatg atccatgcca gcctgaccaa ctcgaagatc cagggcaaat
    28501 atcgccactc tttctggagt gaaatctacg aaacggtgct ggcgtggtat atcgcaccac
    28561 cgacgctggt ggcgctgatt aacccgcaca aaggcaaatt taacgtcacc gccaaaggtg
    28621 gactggtgga agaagagtac gtcgactggg tgatctcgcg gccctacatc ttccttgttc
    28681 tactcaacct ggtgggcgtt gcggtaggca tctggcgcta cttctatggc ccgccaaccg
    28741 agatgctcac cgtggtcgtc agtatggtgt gggtgttcta caacctgatt gttcttggcg
    28801 gcgcagttgc ggtatcggta gaaagcaaac aggtacgccg atcgaaccgc gtggagatga
    28861 cgatgcccgc ggcaattgcc cgcgaagatg gtcacctctt ctcgtgtacc gttcaggatt
    28921 tctccgacgg tggtttgggg atcaagatca acggtcaggc gcagattctg gaagggcaga
    28981 aagtgaatct gttgcttaaa cgcggtcagc aggaatacgt cttcccgacc caggtggcgc
    29041 gcgtgatggg taatgaagtt gggctgaaat taatgccgct caccacccag caacatatcg
    29101 attttgtgca gtgtacgttt gcccgtgcgg atacatgggc gctctggcag gacagctacc
    29161 cggaagataa gccgctggaa agtctgctgg atattctgaa gctcggcttc cgtggctacc
    29221 gccatctggc ggagtttgcg ccttcttcgg tgaagggcat attccgtgtg ctgacttctc
    29281 tggtttcctg ggttgtatcg tttattccgc gccgcccgga gcggagcgaa acggcacaac
    29341 catcggatca ggctttggct caacaatgat gataacgcga tgaaaagaaa actattctgg
    29401 atttgtgcag tggctatggg gatgagtgcg ttcccctctt tcatgacgca ggcgacgcca
    29461 gcaacgcaac cactgatcaa tgctgagcca gctgtagccg cccagacgga acaaaatccg
    29521 caggtggggc aagtgatgtc aggcgagcag ggagctgatg cccccatcgt ggcgcagaac
    29581 ggtccttcgc gtgatgtgaa gctgaccttt gcgcaaatcg cgccgccgcc gggcagcatg
    29641 gtgctacgtg gtattaaccc gaacggcagc attgagtttg gtatgcgcag cgatgaagtg
    29701 gtgacgaagg cgatgctcaa cttcgaatac acgccatcgc catcgttact gcctgtccag
    29761 tcgcagttaa aggtttatct caatgatgag ctgatgggcg tgctgccagt gaccaaagaa
    29821 cagttgggta aaaaaacgct ggcgcaaatg cccattaatc cactgtttat taccgacttc
    29881 aaccgtgtgc ggctggagtt tgtcggccat tatcaggacg tgtgcgaaaa cccggccagc
    29941 accacgcttt ggctggatgt tgggcggagc agtggactgg atctgaccta tcagaccctg
    30001 aatgtgaaga atgacctgtc acacttcccg gtgccattct ttgacccgcg cgataaccgt
    30061 actaacacct tgccgatggt ctttgcgggt gcgccggatg ttgggctgca acaagcatct
    30121 gccattgtcg cctcgtggtt tggttcgcgt tctggctggc gtgggcagaa cttcccggtg
    30181 ctctataacc aattgccgga tcgcaatgcc attgtctttg caaccaacga caaacggccg
    30241 gacttcctgc gcgatcatcc ggcggtaaaa gccccggtga ttgagatgat taaccatccg
    30301 cagaatcctt acgtcaaact gctggtggtg tttggtcgtg acgacaaaga cctgttgcag
    30361 gcagcgaaag gtatcgctca gggtaacatt ctgttccgtg gtgaaagcgt ggtagtgaat
    30421 gaagtgaaac cgctgctacc gcgtaagccg tacgatgcgc cgaactgggt acgtaccgat
    30481 cgtccggtca catttggcga actgaaaacc tatgaagaac agttacaatc cagcggcctt
    30541 gagccagcag cgattaacgt ttcgctaaac ctgccgccgg atctctacct gatgcgcagt
    30601 accggcattg atatggatat taattaccgc tacaccatgc cgccggtgaa agacagttcg
    30661 cggatggata tcagcctgaa taaccagttc ctgcaatcct tcaacctgag cagcaaacag
    30721 gaggcgaacc gtctgctgct gcgtattccg gtattacaag gtttgctgga tggcaaaaca
    30781 gatgtctcta ttccggcgct gaaactgggc gcgaccaacc agctgcgctt cgactttgag
    30841 tatatgaacc cgatgccagg cggttcggta gataactgta ttaccttcca gccggtgcag
    30901 aatcatgtgg tgattggtga cgattccacc atcgacttct cgaagtatta ccacttcatc
    30961 ccgatgccgg atctacgcgc ctttgctaac gcgggcttcc cattcagccg gatggcggat
    31021 ctgtcgcaaa ccatcaccgt gatgccgaaa gcgcctaacg aagcacagat ggaaacgttg
    31081 ctgaatactg ttggttttat cggcgcacag acgggcttcc cggcgattaa tctgacggtg
    31141 accgatgatg gcagcaccat tcagggcaaa gatgccgaca tcatgatcat cggtggtatc
    31201 ccggacaaac tgaaagacga taagcagatc gacctattgg tgcaggcgac cgaaagctgg
    31261 gtgaaaacac cgatgcgcca gaccccgttc cccggcattg tgccggacga gagcgatcgc
    31321 gcggcagaaa cccagtcaac tctgacctct tccggtgcga tggcggcggt gattggcttc
    31381 cagtcgccgt ataacgacca gcgcagcgtg attgcgctgt tggcagatag cccacgcggt
    31441 tatgaaatgc ttaacgatgc ggtgaacgat agcggcaaac gcgccaccat gttcggttcg
    31501 gtcgcggtga tccgcgagtc cggtatcaac agcctacgtg ttggcgacgt ttattacgta
    31561 ggtcatctgc cgtggttcga gcgcttgtgg tatgcgctgg caaaccatcc gattctgctg
    31621 gcggtgctgg cgactatcag tgtgatattg ctggcatggg tactgtggcg tctgctgcga
    31681 attattagta gtcgtcgtcg tcttaacccg gataacgagt aattgaagat gaatgtgttg
    31741 cgtagtggaa tcgtgacgat gctgttgttg gctgccttta gtgttcaggc agcctgtacc
    31801 tggcctgcct aggagcagtt taaaaaggat tacatcagtc aggaagggcg cgtcatcgac
    31861 cccagcgacg cgcgcaaaat caccacctcc gaagggcaaa gttacggtat gttctttgcc
    31921 ctggcggcta acgaccgtgc agctttcgat aatattctcg actggacgca gaacaatctc
    31981 gctcagggtt ctttaaaaga acgtttgccc gcctggctgt ggggcaagaa agagaacagt
    32041 aagtgggaag tgctggacag caattcggcc tccgatggtg atgtctggat ggcctggtcg
    32101 ttgctggagg cggggcgttt gtggaaagag cagcgttata ccgacatcgg cagcgcgttg
    32161 ctaaaacgta tcgcgcggga ggaagtggtg acggtgcctg ggctgggttc catgttgtta
    32221 ccgggcaaag tgggttttgc tgaggataac agctggcgtt ttaaccccag ctacatgccg
    32281 ccgacgctgg cgcagtattt cacccgcttt ggcgcgccgt ggaccacgct gcgcgaaacc
    32341 aatcaacatt tattgctgga aaccgccccg aaaggctttt cgccagactg ggtgcgctat
    32401 gagaaagaca aaggctggca gctaaaagcc gaaaaaacat tgatcagcag ctacgacgct
    32461 atccgcgttt acatgtgggt aggcatgatg cctgacagcg atccgcaaaa agcgcggatg
    32521 ctcaaccggt ttaaaccgat ggcgacattc actgagaaaa acggttatcc gccggaaaaa
    32581 gtggatgtgg ctacggggaa agcgcagggt aaaggaccgg tcggtttttc tgccgccatg
    32641 ctgccttttt tacaaaaccg cgatgcgcag gcagttcagc gccagcgcgt ggccgataac
    32701 tttcccggca gcgataccta ttacaactat gtgctgaccc tgtttggaca aggctgggat
    32761 caacaccgtt tccgcttctc gacaaaaggt gagttattac ctgactgggg ccaggaatgc
    32821 gcaaattcac actaaacata ttcacgcttt ccctcggtct ggccgtcatg ccgatggtcg
    32881 aggcagcacc aaccatcagc agaccctgcg gatggacagc ggcaacacta atgccgtgcg
    32941 cgggctggcg aatatttacc gccagcagtc gccagaaaaa gctgaagcgt ttatcgcctc
    33001 gctctctgcc agccagcggc gcagcattga tgatatcgaa cgcagcctgc aaaacgaccg
    33061 tctggcacag caggcagagg tactggaaaa ccagggcaaa tgggcgcagg cggcagcact
    33121 tcagcggcaa cgactggcgc tgggccccgg cagcgtatgg attacttacc gactttcgca
    33181 ggatctctgg caggccggac aacgcagcca ggccgatacg ttaatgcgca atctggcgca
    33241 gcagaagccg aacaacccgg agcaggttta cgcttacggg ctgtacctct caggtcacaa
    33301 ccaggacaga gcggcgctgg cgcatatcaa cagcctgccg cgcgcgcagt ggaacagcaa
    33361 tattcaggag ctggttaatc gactgcaaag cgatcaggtg ctggaaaccg ctaatcgcct
    33421 gcgagaaagc ggcaaagagg ctgaagcgga agcgatgctg cgtcagcaac caccttccac
    33481 gcgtattgac ctcacgctgg ctgactgggc gtaacaacga cgtgattaca ccgccgcccg
    33541 cactgcatat cagaatgtcc tgacgcggga gccaactaac gccgatgcca ttcttggtct
    33601 gacggaagtg gatattgctg ccggtgacaa agcggcggca cgtagccagc tggcgaaact
    33661 gcctgccacc gataacacct cgctgaacac tcagcggcgc gtggcgctgg cacaggcgca
    33721 gcttggagat accgcagcgg cgcagcagac gtttaataag ttgatcccgc aggcaaaatc
    33781 tcagccaccg tcgatggaaa gcgcgatggt actgcgcgac ggtgcgaagt ttgaagcgca
    33841 ggcgggcgat ccaaagcagg cgctggaaac ctacaaagac gccatggtcg catccggtgt
    33901 gactacgacg cgtccgcagg ataacgacac ctttacccga ctgacccgta acgacgagaa
    33961 agatgactgg ctgaaacgcg gcgtgcgcag cgatgcggcg gacctctatc gccagcagga
    34021 tcttaacgtc acccttgagc acgattactg gggttcgagc ggcaccggtg gttactccga
    34081 tctgaaagcg cacactacca tgttgcaggt ggatgcgccg tattctgacg ggcggatgtt
    34141 ctttcgcagt gatttcgtca atatgaacgt cggcagtttc tccactaatg ccgatggcaa
    34201 atgggatgac aactggggca cctgtacatt acaggactgt agcggcaacc gcagccagtc
    34261 ggattccggt gccagcgtgg cggtcggctg gcgaaatgac gtctggagct gggatatcgg
    34321 taccacgccg atgggcttca acgtggtgga tgtggtcggc ggcatcagtt acagcgatga
    34381 tatcgggccg ctgggttaca ccgttaacgc ccaccgtcgg cccatctcca gttctttgct
    34441 ggcctttggt gggcaaaaag actccccgag caataccggg aaaaaatggg gtggcgtacg
    34501 tgccgacggt gtggggctaa gtctgagcta cgataaaggt gaagtaaacg gcgtctgggc
    34561 atcgcttagt ggcgaccagt taaccggtaa aaatgtcgaa gataactggc gcgtgcgctg
    34621 gatgacgggc tattactata aggtcattaa ccagaacaat cgccgcgtca caatcggcct
    34681 gaacaacatg atctggcatt acgacaaaga tctgagtggc tactcactcg gtcagggcgg
    34741 ttactacagt ccgcaggaat acctgtcgtt tgccataccg gtgatgtggc gggagcgcac
    34801 ggaaaactgg tcgtgggagc tgggggcgtc tggctcgtgg tcgcattcac gcaccaaaac
    34861 catgccgcgt tatccgctga tgaatctgat cccgaccgac tggcaggaag aagctgcgcg
    34921 gcaatccaac gatggcggca gcagtcaggg cttcggctac acggcgcggg cattacttga
    34981 acgacgtgtt acttccaact ggtttgttgg tacggcaatt gatatccagc aggcgaaaga
    35041 ttacgcaccc agccatttcc tgctctacgt acgttattcc gccgccggat ggcagggtga
    35101 catggattta ccgccgcagc cgctgatacc ttacgccgac tggtaagttt tcagatagcg
    35161 cctctcttaa tgccgctgcg atcgggtata ctcgggcggc aatctgggat ttccgggggg
    35221 agacaatttg cgcgtaagtc gctcgttaac aatcaagcag atggcaatgg tggcagccgt
    35281 tgtcctggtg ttcgttttta ttttttgcac cgttttgctg ttccatctgg tccagcagaa
    35341 tcgctataac acggctacgc aactggaaag cattgctcgc tctgtccgcg aacccttatc
    35401 ttcagctatt ttgaaaggcg atattcccga agcggaagct attcttgcca gcattaaacc
    35461 ggcaggcgtg gtcagccgtg ccgatgtagt gctgcctaac cagttccagg cgctgcgtaa
    35521 aagttttatt ccagagcgtc cggtgccggt aatggttact cgcctgtttg agctaccggt
    35581 tcaaatctcg ctgggcgtct actcgctcga acgtccggca aatccgcagc caatagccta
    35641 tctggtgcta caggcggatt ccttccgtat gtataagttc gtgatgagca ctctctcaac
    35701 gttagtgacc atttacttac ttttgtcgct tatcctgacc gtcgccatca gctggtgcat
    35761 taaccgcctg attttgcatc cgttacgcaa tattgctcgc gaacttaacg ccatcccagc
    35821 ccaggagctt gttggtcacc aactggcatt accgcgtctg catcaggacg atgaaatcgg
    35881 tatgttggtg cgcagttaca acctcaacca gcaattgctg cagcgccatt atgaagaaca
    35941 gaacgaaaat gcgatgcgct tcccggtgtc ggatttgccg aacaaagcct tgctgatgga
    36001 gatgctggag caggttgtcg cgcgtaaaca aaccaccgcg ctgatgatca tcacctgtga
    36061 aaccctgcgt gatactgcgg gcgtgctgaa agaggcgcaa cgagaaattc tgctgctgac
    36121 gctggtggaa aaactcaaat cggtactgtc gccacgtatg atcctcgcgc agattagcgg
    36181 ttatgacttt gctgtcattg ccaacggtgt acaggaaccg tggcacgcaa tcactttggg
    36241 tcagcaagta ctcactatca tgagcgagcg cctgccgatt gaacgtattc aactccgtcc
    36301 gcactgtagc attggcgtgg cgatgttcta cggcgatctc accgccgaac agctttacag
    36361 tcgcgctatt tctgcggcat ttaccgctcg ccataaaggc aagaatcaga ttcagttctt
    36421 tgatccgcag cagatggaag cagcccagaa gcggttgacg gaagagagcg atatccttaa
    36481 tgcactggaa aatcatcagt ttgcaatttg gttacagcca caggtcgaga tgaccagcgg
    36541 taaactggtc agtgcggaag tgttactgcg tatccagcaa ccggatggca gttgggacct
    36601 gccggatggc ttaatcgatc gcattgagtg ctgtgggctg atggttaccg tcggtcactg
    36661 ggtgctggaa gagtcctgtc gattgcttgc agcctggcaa gagcgcggca ttatgctgcc
    36721 cttgtcggta aacctctctg cgctgcaact gatgcacccg aatatggtgg cggatatgct
    36781 ggaactgtta acccgctatc gcattcagcc gggaacactg attctggaag tgacagaaag
    36841 ccgacgtatt gacgaccctc atgctgcggt ggcaatcctc cgtccgctgc gcaatgccgg
    36901 agttcgggtg gcgctggatg atttcggcat gggctacgca gggctgcgtc agctgcagca
    36961 tatgaaatcg ttgccaatcg acgtactgaa aatcgacaaa atgtttgttg aaggcttgcc
    37021 ggaagatagc agcatgattg ctgcaattat catgctggcg cagagcctga acttacaaat
    37081 gattgccgaa ggcgtggaga ctgaagcaca acgagactgg ctggcaaaag cgggcgttgg
    37141 tattgcccag ggcttccttt ttgctcgccc actccctatt gaaatcttcg aagagagtta
    37201 cctggaagaa aagtagctac cccaaactga ttacaaaact ttaaaaagtg ctggtttgtg
    37261 cgagccagct caaacttttt aacctttttg tttcaattat gatccaggta catttctgtg
    37321 atgttgtctg ggtgttattt taaggccgca ggtacccccc cataacctta caagacctgt
    37381 ggttttacta aaggacaccc tatgaaaacc tctctgttta aaagccttta ctttcaggtc
    37441 ctgacagcga tagccattgg tattctcctt ggccatttct atcctgaaat aggcgagcaa
    37501 atgaaaccgc ttggcgacgg cttcgttaag ctcattaaga tgatcatcgc tcctgtcatc
    37561 ttttgtaccg tcgtaacggg cattgcgggc atggaaagca tgaaggcggt cggtcgtacc
    37621 ggcgcagtcg cactgcttta ctttgaaatt gtcagtacca tcgcgctgat tattggtctt
    37681 atcatcgtta acgtcgtgca gcctggtgcc ggaatgaacg tcgatccggc aacgcttgat
    37741 gcgaaagcgg tagcggttta cgccgatcag gcgaaagacc agggcattgt cgccttcatt
    37801 atggatgtca tcccggcgag cgtcattggc gcatttgcca gcggtaacat tctgcaggtg
    37861 ctgctgtttg ccgtactgtt tggttttgcg ctccaccgtc tgggcagcaa aggccaactg
    37921 atttttaacg tgatcgaaag tttctcgcag gtcatcttcg gcatcatcaa tatgatcatg
    37981 cgtctggcac ctattggtgc gttcggggca atggcgttta ccatcggtaa atacggcgtc
    38041 ggcacactgg tgcaactggg gcagctgatt atctgtttct acattacctg tatcctgttt
    38101 gtggtgctgg tattgggttc aatcgctaaa gcgactggtt tcagtatctt caaatttatc
    38161 cgctacatcc gtgaagaact gctgattgta ctggggactt catcttccga gtcggcgctg
    38221 ccgcgtatgc tcgacaagat ggagaaactc ggctgccgta aatcggtggt ggggctggtc
    38281 atcccgacag gctactcgtt taaccttgat ggcacatcga tatacctgac aatggcggcg
    38341 gtgtttatcg cccaggccac taacagtcag atggatatcg tccaccaaat cacgctgtta
    38401 atcgtgttgc tgctttcttc taaaggggcg gcaggggtaa cgggtagtgg ctttatcgtg
    38461 ctggcggcga cgctctctgc ggtgggccat ttgccggtag cgggtctggc gctgatcctc
    38521 ggtatcgacc gctttatgtc agaagctcgt gcgctaacta acctggtcgg taacggcgta
    38581 gcgaccattg tcgttgctaa gtgggtgaaa gaactggacc acaaaaaact ggacgatgtg
    38641 ctgaataatc gtgcgccgga tggcaaaacg cacgaattat cctcttaatc tcaccactta
    38701 tgcccgcact cccttccccg agtgcgggtg tttgcgcata attgacccct gtgcctgaac
    38761 ttaccacctg ggtgagttca ggtttatttc acttcttccg tgacattttc atgttcttgc
    38821 ggtctaacac gaagtgtttt taacgtcatt tagactgacc gacaacgggc agttttttta
    38881 ttaccaggat agttgatcag gggttcacat gcagggcata aaaattcgac ttttagcggg
    38941 cggtttgctg atgatggcca ctgctggcta tgtgcaggca gatgcgctcc agcctgatcc
    39001 agcatggcaa caggggacgc tttccaacgg tttacagtgg caagtgctga ccacccccca
    39061 gcgtcccagc gatcgtgttg aaattcgcct gctggttaat accggttcgc tcgccgaaag
    39121 tacacaacag agcggttaca gtcacgccat ccctcgtatt gcgctaacgc aaagcggtgg
    39181 ccttgacgca gcacaggcgc gttcattgtg gcagcagggg atcgacccta aacgcccgat
    39241 gccgccggta attgtctctt atgacaccac gctgtttaat ctgagtttgc ccaataaccg
    39301 taacgatttg ctgaaagaag cgctctctta tctggcaaat gccactggca aattgaccat
    39361 cacaccagaa accatcaacc acgcgctgca aagtcaggac atggtggcaa cctggcctgc
    39421 cgatactaaa gagggctggt ggcgctatcg tctgaaaggg tcaaccttgt taggtcacga
    39481 tcctgccgat ccgctgaaac aacccgttga agcggaaaaa attaaagatt tctatcagaa
    39541 atggtacacc ccggatgcaa tgacgctact ggtggtggga aacgtggatg cgcgctcggt
    39601 tgtcgaccaa atcaacaaaa cgtttggcga actgaaaggc aaacgtgaaa cgccagctcc
    39661 ggtgccgacg ctttctccgc tgcgtgcgga agcggtgagt attatgactg acgcggtgcg
    39721 tcaggaccgg ttatctatca tgtgggatac gccgtggcag ccgattcgtg aatcagccgc
    39781 actgctgcgc tactggcgtg cggacctggc ccgcgaggcg ctgttctggc atgttcagca
    39841 agcgttaagc gccagtgaca gcaaagacat cggtcttgga tttgactgcc gtgtgctgta
    39901 tctgcgtgcg cagtgtgcca tcaacatcga atcaccaaac gacaagctga acagcaacct
    39961 taatctggtg gcgcgtgaac tggcgaaggt tcgcgataaa ggtctgccgg aagaagagtt
    40021 caatgcgtta gtggcgcaaa agaaactgga gctgcagaaa ctgtttgccg cctatgcacg
    40081 ggctgatacc gatattctga tgggccagcg gatgcgttcg ttgcaaaatc aggtcgtcga
    40141 tatcgcgccg gagcagtatc agaaactgcg gcaggatttc cttaacagcc tgactgtgga
    40201 gatgttaaat caggatctgc gtcagcagtt gtcgaatgat atggcgttaa tactgctgca
    40261 gccgaaaggc gagccggaat ttaacatgaa agcgttgcag gcggcctggg atcaaatcat
    40321 ggccccatct accgccgctg cgaccacctc tgtcgccacg gatgacgtac atcctgaagt
    40381 gacggatatt ccacctgcac agtaatgcct gatggtgatg agttagcaca accatcaggt
    40441 atttatggat gagctggata gaggggtaac actttatcca gccttttgca tatgctgcgt
    40501 ttacgctggc atcgcctcac gcgggataat cgcgccgcga tactgaataa cggtacttgc
    40561 ggtcaggtgc ccgcgtttcg ccgcgttttc cgcactgccg cccgtcaaac gtaccgccag
    40621 ataaccggcg ctgaaagagt cgccagctgc ggtggtatcg atcacttttt ctttcggcag
    40681 tttcaccgcc ggaacatcaa ctaacgcttc accagcaatg gacaccaggc aagaatccgc
    40741 cccgcgtttc accaccactt ctttcacgcc cgcgttatgg gtgcgcgcaa tgacgtcttc
    40801 caccggctgt tgaccccaca gcgcgtcttc gtcgtccagc gtcaggaagg cgatatccgt
    40861 gcattccagc atttgctggt acacctgctg tgtctcttct ttgctggccc acaggcgcgg
    40921 acgatagtta ttgtcgaaaa tcacttttcc gcctttggcg cggcattcgc gcagcaggga
    40981 aagcagcttt tcgcggctgg tcgggcttaa gatcgccagg ctaatcccgc tcaggtagag
    41041 ataatcgaaa ttcgccagct cttcgcaaat cgccgcagac tgctcgctcg ccagccagaa
    41101 tttggcggcg gcttcgttac gccagtagta gaacgtacgt tcgccggtgc tgtcggtttc
    41161 aatgtagtaa aggcccggaa gacggttttc catccgttgg gtcagggaag tatcaacgtt
    41221 ctcgccgtgc caggcgtcca gcatctgctg gctaaaactg tccgttccca gcgccgttac
    41281 gtaatgaacg gttaatgccg caggatcgac ctgacgggcg atatagacgg aagtgttcag
    41341 ggtatcgccg ccgaaaccgc gcttaacgtc cgcgcctttc tcggaaagct caatcatgca
    41401 ttcgccaatc acggcaatct ttttggacat agtcgtgaac ctgatctgta aaaaattagc
    41461 gttagtttgc gctgtggtgg tttgctggtc aaccatatta aaacagtgtt ccattatttt
    41521 tttgagccag aacgtattcc tgaaagattc cgttgtggag tgaggaaatt taccccggcc
    41581 aggcggtaaa gttctgccct tacgcgccga taatctttgt cgagtccggg cagcatcact
    41641 tttaaacaca ggacatcttt gatgataagg caggttatcc agcgaataag caaccctgaa
    41701 gcaagcatcg agagcttgca ggaacggcgt ttttggttgc agtgtgagcg tgcttacacc
    41761 tggcagccga tctatcaaac atgcgggcgg ttaatggccg tggagctatt aacggtggtc
    41821 acgcatccct tgaacccttc gcaacgcctg ccgccggatc gctattttac tgaaatcacc
    41881 gtcagccatc ggatggaggt tgtgaaagag cagattgatt tgctggcgca aaaagccgac
    41941 ttctttgtag agcacggcct gctggcatcg gtcaatattg atggccctac gctcatcgcc
    42001 ctgcgtcagc aaccaaaaat cctgcgccag attgagcgtc ttccctggct gcgtttcgaa
    42061 ctggtggagc atatccgtct gccgaaagat tcaacctttg cctcgatgtg tgaatttggc
    42121 ccgctgtggc tggatgattt tggtaccggg atggcaaatt tctctgcact aagtgaagtg
    42181 cgttatgact acatcaaaat cgcgcgagaa ctgtttgtga tgctgcgtca gtcgccggaa
    42241 ggccgcacac tcttttctca gcttttacat ctaatgaatc gctattgtcg cggggtgatt
    42301 gtcgagggcg tagaaacacc ggaagagtgg cgtgatgttc agaactcgcc cgcattcgcc
    42361 gcacaaggct ggtttctttc acgcccggca ccgatggaaa cgctgaatac ggcggttctg
    42421 gcgctataag ctgcctcatt ttccgccggg ctggactatc tttaggactg gcacaggaag
    42481 aatatgagga agcgaacgat gagcaaggca ggcaaaataa ccgctgcgat ttcaggggct
    42541 ttcttgttgt tgattgtcgt ggcgatcatt ttgattgcaa cttttgactg gaatcgactc
    42601 aaaccgacca tcaaccagaa agtctctgcg gagttgaatc gtccgttcgc tatccgtggc
    42661 aatctgggcg tggtgtggga gcggcaaaaa caagaaactg gctggcgcag ctgggtgccg
    42721 tggcctcatg tacatgcaga agacatcatt cttggtaatc caccggatat tcccgaagtc
    42781 acgatggtgc atttgccgcg agtagaggca acgctggcac cgctggcgct gctgaccaaa
    42841 acggtctggc tgccgtggat caagctcgaa aagcccgacg cgcgcctgat tcgcctctct
    42901 gaaaagaaca ataactggac gtttaatctt gccaacgatg acaacaaaga cgcgaatgca
    42961 aagccgtcgg catggtcgtt tcggctggat aatattcttt tcgatcaagg gcggatcgcc
    43021 attgatgaca aagtaagcaa agcggatctg gaaatttttg ttgatccgtt aggcaagccg
    43081 ctgccattca gcgaagttac tggatcgaaa ggtaaagcgg ataaagaaaa ggtgggcgat
    43141 tacatttttg gcctgaaggc gcagggacgt tataacggtg aaccgctcac gggtacggga
    43201 aaaataggcg gtatgctggc gctgcgtggc gaagggacgc cgtttccggt acaggctgat
    43261 ttccgttcag gtaatacccg tgttgctttt gatggcgtcg tgaatgaccc aatgaagatg
    43321 ggcggtgtcg atttacggct taaattttct ggcgattcac tgggtgatct ctatgaactg
    43381 acgggcgttc tgctgcccga taccccgccg tttgaaacgg atggtcggct ggtagcgaaa
    43441 atcgacactg aaaaatcgtc ggtctttgat tatcgcggtt ttaatgggcg aattggcgat
    43501 agcgatatcc acggttctct ggtctacacc accggaaagc cacgaccaaa actggaaggt
    43561 gatgtcgagt cgcggcaatt gcggctggcg gacctgggac cgttgattgg cgttgattcc
    43621 gggaaagggg cagaaaagtc gaaacggtct gaacagaaga agggcgaaaa aagcgttcag
    43681 cctgcgggca aagtgctgcc ttatgaccgc ttcgaaaccg ataaatggga cgttatggat
    43741 gccgatgttc gcttcaaagg gcggcgcatt gagcatggca gtagcctgcc gattagcgat
    43801 ctttctactc atatcatcct caaaaatgct gacctgcgcc tgcaaccgct gaaatttggc
    43861 atggcgggcg gcagcattgc ggcgaatatt catctggaag gcgataaaaa gccgatgcag
    43921 gggcgggcag atattcaggc tcgtcgactg aaactgaaag aactgatgcc cgatgtggaa
    43981 ctgatgcaga agacgctggg ggaaatgaac ggtgacgcgg aactacgcgg tagcggtaac
    44041 tcggtggcgg cgcttttagg caacagtaac ggcaacctga aactgttgat gaatgacggg
    44101 ctggtgagcc gcaacctgat ggagattgtt gggctgaatg tcggcaacta cattgtcggt
    44161 gcgatatttg gtgacgatga ggtgcgggtg aactgcgcgg cggcgaatct gaatattgcc
    44221 aacggcgtgg cgcgcccgca gatttttgct ttcgatactg agaacgcgtt gattaatgtt
    44281 accggcacgg caagttttgc ttcggaacag ctggatttga ctattgatcc ggagagtaaa
    44341 gggattcgga ttatcacact gcgttcgccg ctgtatgtgc gtgggacgtt taaaaatccg
    44401 caggctgggg tgaaagccgg accgctgatt gcccgtggtg ctgttgctgc ggcactggca
    44461 acgctggtaa caccggcggc ggcgttactg gcactgatct caccttccga aggggaggct
    44521 aatcagtgtc ggacgatatt gtcgcagatg aagaagtgat gataattgcc ggatgtctga
    44581 catccggcaa ttaaacagat ttagattttt ggcaaagtgt gctttgttca tgccggatgc
    44641 ggcgtgaacg ccttatccgg cctacaaatc cttgtgaatt caatatattg tatggaaatt
    44701 cagtcctgat aagcgtagcg catcaggctt ttcacactta ttacaacgac tgatgtcgcg
    44761 tctcatgggt cagcagcagg gcgattaacg tcaagccagc catcgccgcc agatataacc
    44821 ccaccgcacc taacccgtag ttagcctgca accaggctgc gatatacggc gcaacggaag
    44881 ccccgagaat cgacgatacg ttgtaagaga acgatgctcc ggtgtaacgc acttctgtcg
    44941 gaaacagctc tggtaacagc gcacccatcg gcccgaaggt cagccccatc agacttaacc
    45001 ccagcagcag gaaggcaaaa accagaatcg ggttgccaga accgagcagc gggttaaagg
    45061 cgaacagcgc gaacaggatg atcagcgtgg tgatgatcac catgctttta cggcgaccaa
    45121 aggcatcagc cagtaatccg gcgactggca ccatcacgcc aaaaccaata actgccatca
    45181 tcagcatcca caacacttcg ttacgcggca ggccaagccc aactggcgcg gcggcggtac
    45241 tgaaggtcat agagtagacc gtcatgatgt aaaacagcgt ataggttgcc agcataatga
    45301 acgtacccag tacggttacg cgaacatgtt tggtcagcag cgtacccagc gggatcttca
    45361 cctgtttttt cgctttagcg actttctcaa acaccggcga ctcatgcagc gacacgcgaa
    45421 catacaggcc gataatgacc agcaccgccg agaagataaa tggcacacgc cagccccagc
    45481 tcataaactg ctcatcggtc agtagccagg aaagcagcaa gaaagtgccg ttggcaaaga
    45541 agaagccaat cggtgcgcct agctgtggga aggagccata cagtgcacgt ttgcgcggtg
    45601 gggcgttttc agtcgccagc agcgccgcgc cgccccattc accgcctaag cccagaccct
    45661 gaccaaatcg agccagcgcc agcagcagtg gggcgaaaat accaatcgtg gcatagcccg
    45721 gcagcagacc aatcaccacg gtcgaaatcc ccatcgttag caacgaggcg accagcgtcg
    45781 ctttacgccc aacgcgatcg ccaaaatgac caaaaacggc agagccaatg gggcgcgcga
    45841 cgaaggcgat ggcgaaggtg gcgagcgact gtagcgttgc tgccgttgga tcgccctgcg
    45901 ggaagaagat atgcggaaac acaataacgg ccgcagtggc gtaaatgtaa aagtcgaaga
    45961 actcaatggc cgtgccaatg agagaggcga caaggacttt attacgcgag ttgatcggcg
    46021 tgtattcttg ttcgtggtcg agtgttgtgg ctgttgcttg cataatcttt tcttattttt
    46081 tggctaacga atagccatat tacgcacagc aaaagtgata tttcaatgtg taacaaatcg
    46141 cggaaatggt caaaaaccag gcaaaaaggg gataatgttt aggccagtga tttttcttct
    46201 atgaaatgct tcacataatt ttataaatag tggataaaac tggtttcagg ttaaataaaa
    46261 gttaccaact ggatttatat gctggggtta tgagttggag tgaaattgtt tgctcccaaa
    46321 tagatggctg aagaggctct ggggaagagt ttcaagaggt tatgtgcgca ttatagccgg
    46381 atgcgatgct ggcgcatctt atccggccta cggggagcca ggccgaataa aatcgtatgc
    46441 cggataaggc gtttacgccg catccggcct attttaaggc tgcgttttcc ctggcattcg
    46501 cgggtcgtct ttatattcgg cggtggcaat ccacgccgcg caaaacagcg tcaaacgggc
    46561 gaagaagtag aaaaatgcca tcagccccag cacggaacca aatgctgcgc cagaggggga
    46621 tttcatcagc gatggcaggg tgtaggtcat cacgatttta atcacttcaa aaccaatagc
    46681 agccagaaat gttccgcgaa tcagggcttt tttgcgcgga cggtggcgcg gcaaccgcca
    46741 gaagatccag aagaaaagca gatagttggc gaagatggaa atcgccaggc caatcaatcg
    46801 ccacgtcggt ttcagccact caatgctgtt caggtgcagg gcgctaataa tcatttgctg
    46861 cgccgaaccg gcaaccgagg tgatcgaaag cgtcacaatc agcgcaatca acaaaccaat
    46921 cagtgaaata aaatcacgca gatattttac ccagaacttc tcctgatctt gcggcgagcg
    46981 ttcccagaca tcgcgcgact gggcacgaat cgcttcacgc aggttaccca tccagttgat
    47041 gccggaataa agtgccaccg ccaggccgac aagccctaca gtcgtacgct gctgaacggc
    47101 ggtgttgatg gtgtttttca acgtggcggc tagtgtcgga tcgctgatgt tttgcagaat
    47161 tttgtcgaag atatcctgta gcaacatcgg atgggaggcc agcacaaagc cccctgcggc
    47221 aaacgacacc atcaaaatcg ggatcatcga caaaaatgag aaataggtga tagccgcacc
    47281 aaactggtta cccagccgat cgttaaagcg ttctgtcgcg cgaataaggt gcgcaataac
    47341 cggttggcgt tggacttttt cggcagtggt ggtgacggtt tccagcgcct ggctaacttt
    47401 gccgcctttc tcgctattat ccaactgctt aatcggctcg tgctccagat cctgggtggg
    47461 acgtttgatc tcgttttcct gcgtcatccg ctcttttgtc cttctgtcgt ggtcattctt
    47521 aaaaagtata gtcggtcagt ccaggtgctc tttcattacg ccagccagcc actgcataaa
    47581 caggtttaca cgccgggaaa gctcccgacg ctgcgggtaa accagcgaaa gggagagcgg
    47641 ctcggcacgg tagcctggca acacttcaat aagccgcccg gcacgcaggg cttcgcgcac
    47701 ggcaatgcgc gggatctgaa taatccccag accggcaaga ccggcggcga gatagttttc
    47761 gctactgttt accgtcaaca tgccgccaga cttaaaccac tggacgccat taacgctggc
    47821 aacctcaaaa cctaacggat gtacacccag gtgcggtgta taacgcacta ttgcgtgtga
    47881 agtcagatca tcggggcttt gcggataacc aaagcgcgtc agatagtgcg gactggcaca
    47941 gttgaccatg gttagtttgc cgaggggacg ggcgataacg ccatcttccg gtaacgcgcc
    48001 agtgcgtatc acgcaatcaa aaccatcatg aagaatatct accggacggt tatggctact
    48061 cagttccagc tcaattcccg gatgctgata gagaaattcc gacagacgcg gcagtaacag
    48121 gtttttcgcg attcctgacg ggatgtcgat gcgtaattta ccgctgatac tggtggcatc
    48181 ctgttgaaac agaccgtcca gttcgctgag attactcaac acatcttttg cccgttgata
    48241 ataggtcatg ccttctggcg tgagtttgac ccgtcgcgta gtgcgctgga gaagctgggt
    48301 gccaagttga tgttccagtg cctgtatctg gcgcgaaaca cttccctttg gcaaagcaaa
    48361 gaaatccgcc gcgcgggaaa aactttccag ctccgcgact ttgatgaaca actgcattgc
    48421 gtgaatttta tccatttctt tgaccgattg ttgttcacag cgaaacagta acgcgcatat
    48481 cgcattgttt attgtttttt ctaacatcta atgccgggtt tcacaatgtt gtcatgacga
    48541 atgtggcgat ttctcagcag gaaaagggcg gaatagcttt atgagaaagg tgaatcccct
    48601 taaacagagc gtatttttca gtgattccct ttaaagtgaa tagaggacgt attggctatt
    48661 tgtgcttatt tttgtctcat tgccatttgt tattttggct tgtagtcgcg gaatttcatt
    48721 tttaattgca tgcgtactaa ccaccaatat aaggaaatcc tcaaaattgt tttaaatagt
    48781 gccctcgaca tgtaatttca ggcgtttagc tgtaactatt tatatcggta actattttct
    48841 acgcgtcatc tctgtagaat ggccaccgcc aaatggcgaa ttcttatttt ttctcgcggg
    48901 taataccctt ccactactta agtcttttta acgtattcag gtggagattc agttatgcaa
    48961 atagtcatgt ttgacaggca gtcaatattt attcatggaa taaaaatcag tttacagcag
    49021 cgtattccag gagtgagtat tcagggggtc agtcaggcag acgagttatg gcaaaagctg
    49081 gaaagttacc ctgaagcctt agttatgctc gatggcgatc aggatggtga gttttgctac
    49141 tggttgctgc aaaaaaccgt ggtgcagttt cctgaggtta aggtgttaat cacggcgacg
    49201 gattgcaata aacggtggtt acaggaagtt atccatttta atgtgctggc cattgtgcct
    49261 cgcgattcaa ccgtcgagac atttgcgttg gcggtaaata gtgcggcgat ggggatgatg
    49321 tttctgcctg gagactggcg gacgacgccg gaaaaggaca ttaaagatct caaatcgtta
    49381 agcgcccgcc agcgggagat tttaaccatg ttagcggcgg gcgaatcaaa taaggagatc
    49441 ggcagagcgc tgaatatcag taccggaacg gtaaaagcgc atctggaatc cctgtatcgc
    49501 cgtctggaag tgaaaaatcg cacccaggcg gcaatgatgt taaatatctc ctcctg
//
LOCUS       DAOFMX010000022        48337 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.022, whole genome shotgun sequence.
ACCESSION   DAOFMX010000022 DAOFMX010000000
VERSION     DAOFMX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.022"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            187..480
                     /locus_tag="QBA20_001439"
     CDS             187..480
                     /locus_tag="QBA20_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDQ6292029.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="QBA20_001440"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="QBA20_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDQ6292030.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="QBA20_001441"
     CDS             complement(1922..2374)
                     /locus_tag="QBA20_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDQ6292031.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="QBA20_001442"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="QBA20_001442"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDQ6292032.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="QBA20_001443"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="QBA20_001443"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDQ6292033.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="QBA20_001444"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="QBA20_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDQ6292034.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="QBA20_001445"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="QBA20_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDQ6292035.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="QBA20_001446"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="QBA20_001446"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDQ6292036.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="QBA20_001447"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="QBA20_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDQ6292037.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="QBA20_001448"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="QBA20_001448"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDQ6292038.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="QBA20_001449"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="QBA20_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDQ6292039.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="QBA20_001450"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="QBA20_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDQ6292040.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="QBA20_001451"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="QBA20_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDQ6292041.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="QBA20_001452"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="QBA20_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDQ6292042.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="QBA20_001453"
     CDS             complement(15085..15582)
                     /locus_tag="QBA20_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDQ6292043.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="QBA20_001454"
     CDS             complement(15579..15941)
                     /locus_tag="QBA20_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDQ6292044.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="QBA20_001455"
     CDS             complement(15931..16278)
                     /locus_tag="QBA20_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDQ6292045.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="QBA20_001456"
     CDS             16387..16800
                     /locus_tag="QBA20_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292046.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="QBA20_001457"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="QBA20_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="QBA20_001458"
     CDS             complement(18324..20039)
                     /locus_tag="QBA20_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDQ6292047.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="QBA20_001459"
     CDS             20206..21099
                     /locus_tag="QBA20_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDQ6292048.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="QBA20_001460"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="QBA20_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="QBA20_001461"
     CDS             complement(21910..23526)
                     /locus_tag="QBA20_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDQ6292049.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLYVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="QBA20_001462"
     CDS             23821..24495
                     /locus_tag="QBA20_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDQ6292050.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="QBA20_001463"
     CDS             complement(24534..26195)
                     /locus_tag="QBA20_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDQ6292051.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLVLRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="QBA20_001464"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="QBA20_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDQ6292052.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="QBA20_001465"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="QBA20_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDQ6292053.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27338..27410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27649..27981
                     /locus_tag="QBA20_001466"
     CDS             27649..27981
                     /locus_tag="QBA20_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDQ6292054.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29227)
                     /locus_tag="QBA20_001467"
     CDS             complement(27983..29227)
                     /locus_tag="QBA20_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDQ6292055.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTNTWTPDSQWLVFDVRPSG
                     ASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYD
                     FHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQ
                     LDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEE
                     GWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRG
                     VVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPR
                     KLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVF
                     SPDGQWLAWMEGGQLWITETDR"
     gene            29328..30428
                     /gene="cbrA"
                     /locus_tag="QBA20_001468"
     CDS             29328..30428
                     /gene="cbrA"
                     /locus_tag="QBA20_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDQ6292056.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30389..31726)
                     /locus_tag="QBA20_001469"
     CDS             complement(30389..31726)
                     /locus_tag="QBA20_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6292057.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31801..32949)
                     /gene="dgoD"
                     /locus_tag="QBA20_001470"
                     /pseudo
     CDS             complement(31801..32949)
                     /gene="dgoD"
                     /locus_tag="QBA20_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32946..33563)
                     /gene="dgoA"
                     /locus_tag="QBA20_001471"
     CDS             complement(32946..33563)
                     /gene="dgoA"
                     /locus_tag="QBA20_001471"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDQ6292058.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33547..34425)
                     /gene="dgoK"
                     /locus_tag="QBA20_001472"
     CDS             complement(33547..34425)
                     /gene="dgoK"
                     /locus_tag="QBA20_001472"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDQ6292059.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34422..35111)
                     /gene="dgoR"
                     /locus_tag="QBA20_001473"
     CDS             complement(34422..35111)
                     /gene="dgoR"
                     /locus_tag="QBA20_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDQ6292060.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35389..36045
                     /locus_tag="QBA20_001474"
     CDS             35389..36045
                     /locus_tag="QBA20_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292061.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGCSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36091..36903)
                     /gene="yidA"
                     /locus_tag="QBA20_001475"
     CDS             complement(36091..36903)
                     /gene="yidA"
                     /locus_tag="QBA20_001475"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDQ6292062.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37017..37415)
                     /locus_tag="QBA20_001476"
     CDS             complement(37017..37415)
                     /locus_tag="QBA20_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDQ6292063.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37655..40069)
                     /gene="gyrB"
                     /locus_tag="QBA20_001477"
     CDS             complement(37655..40069)
                     /gene="gyrB"
                     /locus_tag="QBA20_001477"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDQ6292064.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40098..41171)
                     /gene="recF"
                     /locus_tag="QBA20_001478"
     CDS             complement(40098..41171)
                     /gene="recF"
                     /locus_tag="QBA20_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDQ6292065.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41171..42271)
                     /gene="dnaN"
                     /locus_tag="QBA20_001479"
     CDS             complement(41171..42271)
                     /gene="dnaN"
                     /locus_tag="QBA20_001479"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDQ6292066.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42276..43679)
                     /gene="dnaA"
                     /locus_tag="QBA20_001480"
     CDS             complement(42276..43679)
                     /gene="dnaA"
                     /locus_tag="QBA20_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDQ6292067.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43976..44056
                     /locus_tag="QBA20_001481"
     CDS             43976..44056
                     /locus_tag="QBA20_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292068.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44286..44426
                     /gene="rpmH"
                     /locus_tag="QBA20_001482"
     CDS             44286..44426
                     /gene="rpmH"
                     /locus_tag="QBA20_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDQ6292069.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44443..44802
                     /gene="rnpA"
                     /locus_tag="QBA20_001483"
     CDS             44443..44802
                     /gene="rnpA"
                     /locus_tag="QBA20_001483"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDQ6292070.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44766..45023
                     /gene="yidD"
                     /locus_tag="QBA20_001484"
     CDS             44766..45023
                     /gene="yidD"
                     /locus_tag="QBA20_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDQ6292071.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45026..46672
                     /gene="yidC"
                     /locus_tag="QBA20_001485"
     CDS             45026..46672
                     /gene="yidC"
                     /locus_tag="QBA20_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDQ6292072.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46778..48142
                     /gene="mnmE"
                     /locus_tag="QBA20_001486"
     CDS             46778..48142
                     /gene="mnmE"
                     /locus_tag="QBA20_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDQ6292073.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11989 a        13061 c        12220 g        11067 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaacct ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 gcggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttgg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcaaaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat tcccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatacaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcacg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtaccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtattggtat
    29161 tggtattggt attggtattg gtattggtga gcaggtgatt acggggagca aaggttatct
    29221 gtttcatggc gcggtcctga tacaaaacgg ttcccgccat catacttcac aaggacttca
    29281 ctttcagaca atagtctttt gccatcgtga tgaatgtgag tgaaaagatg gaacatttcg
    29341 acgtggcgat tattggcctc ggcccggcag ggtcggcgtt ggcacgaaag ttagccggca
    29401 aaatgcaggt gatcgcgctg gataaaaagc accagtgcgg tactgaaggt ttcagcaaac
    29461 cttgcggcgg tctgctggca ccggacgcgc agcgatcttt tattcgcgat ggactgacgc
    29521 ttcctgtcga tgtgatcgcc aatccacaga ttttcagcgt caaaactgtc gacgtcgccg
    29581 catcgctcac gcgtaactac cagcgaagct atatcaatat taatcgccat gctttcgact
    29641 tgtggatgaa atcgctgatc cccgccagcg ttgaggttta tcacgatagc ctgtgccgaa
    29701 aaatctggcg tgaggatgat aaatggcatg tcatttttcg tgcagacggc tgggagcaac
    29761 atattaccgc ccgctatctg gtcggtgccg atggcgcaaa ctcgatggtg cggcgacatc
    29821 tctacccgga tcatcaaatc cgtaaatatg tcgctatcca gcagtggttc gcagagaaac
    29881 atccggtgcc gttctactcc tgcatctttg ataatgcgat aactgactgt tactcatgga
    29941 gtatcagcaa agatggttat tttatctttg gcggtgccta tccaatgaaa gacggtcaga
    30001 cgcgtttcac gacgctgaaa gagaaaatga gcgcctttca gttccagttt ggcaaagcgg
    30061 tgaaaagtga gaaatgcacg gtgttgtttc cctcacgctg gcaggatttt gtctgtggta
    30121 aggacaacgc ctttctgatt ggtgaagcgg cgggatttat cagcgccagc tcgctggagg
    30181 ggattagcta tgcgctggat agcgcagaga ttctgcgtgc ggtgttcctg aaacagccgg
    30241 agaagagcaa cgccgcttac tggcgcgcca cacacaaact gcgtttaaaa ctcttcggca
    30301 agatagtaaa aagccgatgc ctgaccgcac cggctttaag aaagtggatt atgcgcagtg
    30361 gtgtggcgca tattccacag ttgaaagatt atccaacgcg cttcacatca cccaccagca
    30421 ggatgtaaga gagcgcgcca atcaacgcga cggcggagat ataaaccagt gcaggcgcga
    30481 aaccgtaacc ctgcgccagg taccccacca ccagcggaac ggtgatgccg cccagaccac
    30541 cggcgaagtt aaacacgccg ccggttaaac caatcaggcg catcggtgcc agagaagaga
    30601 ccagcgacca ggtaatcgaa gcaaaaccgt tgccgaagaa tgccagcgcc atcaggcaca
    30661 taatcatcat cggatcgttg gtgtagttag cgcccataat gcaggtggag atcagcaagc
    30721 cgcagataat cggcgtttta cgcgcaaagc ccagtgaaaa gcccttacgt accagcagat
    30781 ccgcgaccca gccagagagc aggacgccga caaacgccgc gaggaatggc accgtggtca
    30841 taaagcctgc tttcagcgcc gtgattcctt tttcctgggt taaatagttc gggaaccagg
    30901 ttaagaaaaa ccacagtgca gaagccaccg caaattgccc aagataaacg ccgatcagtt
    30961 tacgatggaa caccagtttc cagtctttgg ctgttaacgg ctgacgcgcc tctttcttca
    31021 ccggcgcatc accatccacc agaccgccgc catcacgaat gtaatccagt tcagctttgc
    31081 tgataccttt ggtcaggcgc ggcggctgat aaaccttaaa ccaaatcagc gaccagataa
    31141 tgccgatacc accagtgaca atgaacaccc agtgccagct caacatctcc tgaatccaga
    31201 tcagcagcgg agtcaaaaac gccagaccga caaactgacc agacgtataa aaaccaacgg
    31261 cggaagcgcg ttcatgttcc gggaaccagc tggtcaccat ccggttattg gtcgggaaag
    31321 cgggcgcttc gaaaataccg gttatcgcac gcagaccaat taatgacatt aatcccgtgg
    31381 caaagccttg gaataaagtc accactgacc aaccaaatat cgcaataaaa taagtcacgc
    31441 gagaacctac gcgatctaaa aaccaaccgc cggggatctg gcatagcgta taaagccagg
    31501 cgaaggccga aaatacatag cccatttccg ctttcgtaat gccgaactct tcctgaatat
    31561 gggcggaagc cacggccagg ttagcgcggt caacataaca aatgaccacc gtaataaaga
    31621 tcatcaccag cgtcagataa cgccgacgcc ccggctttgc tgcattaacg ggaatatcca
    31681 tagcgagctt tctccagatt ttgggcatag cgaagccgct caccatgccc tgtaatttac
    31741 agagggttat atttttgtat tgctgtttta gtgcccgatg aggggcttac gtggcaggaa
    31801 ttaccactct gctacgctgt tatcttcatg acgccagagc ggattacgcc agtccggggc
    31861 atttttactg aactcaataa ctttagcttc gtcgatttcc acacctaagc ccggtttcgt
    31921 taacggttta aagaagccgc caaccatgct gaagtcttct ttgtttttca caaagtcgag
    31981 taactccgcg cctttgttgt aatgaatgcc catactttgt tactgaagta ccgcgttata
    32041 ggaaacaaag tcgatatgca ggcaagccgc cagtgcaatc ggtccgaacg gacagtgcgg
    32101 cgcaagggtc acatcatagg cttctgccat tccagcaatt ttgtagcatt cggtaatacc
    32161 gcctgcatgg gagagatccg gttgcagaat cgaaataccg cctgcctcca gcacgcgttt
    32221 aaaatcgaaa cgtgagaaca tgcgctcacc cgccgccagt ggaatatgcg tttgtgccgc
    32281 caatttcggg tagtattcgg cttgttccgc cagcaccggc tcttcaataa acagcgggcg
    32341 atacggctcc agttctttaa tcagcacttt cgccattggc gcgctgacgc gaccgtggaa
    32401 atcaagacca aactcaatct gattgccaaa agcttcacga atttgtgcca cggtgttgac
    32461 tgccgcatct accgcgcggg agttatcaat tagccccagt tcttcacaac cgttcagttt
    32521 gaaggtatcg aagccgattt cgcgcagggt tttaatgccg tcgataacat ccgccggacg
    32581 atcgccgccg acccaactgt aggctttaat tttgtcgcga accaggccgc ccatcagttg
    32641 ccagactggc gcattcagca ctttgccttt gatatcccat aacgcctggt cgatcccggc
    32701 gatggcgctc atcaggattg gaccgccacg ataaaatccg gcgcgataca tcacttgcca
    32761 taagtcgttg atgcgcgaag gatcctgacc aatcaaatag tcacccagct cgtgaactgc
    32821 cgcttccacc gtacgggcgc ggccttcaat cacgggctcg ccccaaccga ccacgccttc
    32881 atcggtttca attttcagga acatccagcg gggaggtaaa cgatacgtgg taattttggt
    32941 gattttcatt gcactgcctc tcgatacgcc ttaacaaatg ctgctgcctg ctgcgcggtg
    33001 cgttctacgg actgcccggc gcgataaaga tcgctgccta agcccgcccc aacacaacct
    33061 gcgtctatcc actgcgccag gttttctggc gtcacgccgc caacggcaaa gactgcgatg
    33121 tccggtggta ataccgcttt taacgctttg atgtattgcg gaccaaaagc cgatgacgga
    33181 aatattttca gcgcctgcgc gcccgcttcg agcgcggtaa aggcttcggt cgccgtcgcg
    33241 cagccggggc aaacggtcat gccgtagccc accgcgcggc ggatcacttc actatggata
    33301 ttgggcgtaa cgatgagctg acagcccatc ctggcgagcg catcgacctg ttcaggtttc
    33361 agtaccgtac ctgcgccaat caacgccttg tcgccatacg catcaacgat ggcgggaatg
    33421 ctctgctccc attgtgggga attcagcggg atttcaaccg cgtcgaaccc ggcgtcaatc
    33481 accgcgccaa catgcgccag cgcctcgtcg ggcgtaatgc cgcgcaaaat ggcgatcagc
    33541 gggagtttag tttgccactg catgagcgat gctccttata ccagcctgaa atgccgtgtc
    33601 gcccgccacc gccgccacgt cgcaacccat cgcctgaaag gcttgctggt agcgcgcggt
    33661 cagcgatgtt ccggcgacaa gggtgatggc gtgttgatgg gtcacatagt cgcgcatact
    33721 ggcaacctct gcgccaatca acaaaccaga gagaaattcg ctgacctgtt cgcggggaag
    33781 tgttcccagc acatgcgagg cgcgaacttc aaaaagctgc ggcaatatgg cgggcgcatt
    33841 aaggccgcgc tcaaggccag ccgcgaaggc atcggcagag ttttcctgcg gcggcaaacc
    33901 tgcgccaatc aatgagtgat ttaacagtaa atgatgtaat tcaccggtca tcacggtgcg
    33961 aaaatcgttg atttgctggc tatcggcctg cacccatttg caatgcgtac cgggcataac
    34021 ataaagagag gaaggagcca gagctcgcgc gccgatcaat tgtgtttctt cgccgcgcat
    34081 cacattgtgg ttatcgtcat gagagacgca taatccggga ataatccaga tattgtcgcc
    34141 aacagacgtt aattgttcgc caatagacga aaaacgggca ggaacagata aatacggagc
    34201 aactttccag ccgacattgc tgccgaccat tcctgccatc accactggcg tattttcttc
    34261 acgccagtcg gtcgtgactt ctgctaacac cgcagccgga gattttccgt tcaggcgcgt
    34321 gacgcctgct tctgattgcc tgctctccag gcagtggtcg ccctgataaa gccaggcgcg
    34381 cagattggtc gatccccagt caattgcgat gtagcgagct gtcatgtgat ttcctttaac
    34441 cttcgtgtcg agctggcaat catggtaagc gccgcctgct ctgccgcatc gccgtcctga
    34501 tgccgtatcg catcgaacag cgccttatgt tcctggagcg tttgcggcat gttggcctca
    34561 tcgcccatcc aggttcgttc aaaaaccgcc cgctgcagcg aactgatcgc aatgctaagt
    34621 tgctgtaaca ccgggttatg caccgactgc agcaccgcct cgtggtagcg aatatccgct
    34681 tcgttaaacg cttcgcggtc ctgattgttg gcaatcatct cgttcagcgc cgattcaatc
    34741 tgcgccagat cgctggaggt cgcgcgctct gctgcccaac gggcaatcgc cggttccacc
    34801 agatttcgca cttcgctcat ggcactgata agccgtgggt cgtagtcgtt ttccagcacc
    34861 cattgcagta cgtcagtgtc gaggtaattc cactggttac gcggtgccac aaacgccccg
    34921 cgataacgtt tcatttcaat cagccgcttc gccatcagcg aacggaacac ctcacggatg
    34981 atgttgcgcg aggttgcaaa ctcttcacag agctccgcct cagccggaag tggcgagcca
    35041 ggtacgtatt tgccgtgaac gatctgttta cccagcgtaa tgacaatgcg atcggtttta
    35101 ttgagagtca tggagagtcc ttgtgctctg tatgttcttc tctactttac cccgatcgat
    35161 gcataacgcg gcaactttgt agtaccagcg tgatgacgtt cgcgtttgcc gcgcgcgtaa
    35221 tgtagtacaa acttatattg ttgtgctaca atttagatca aaaaaagaac aatacataaa
    35281 aaaatgacat gcgtcgggca gaaatctgaa aagggatatc aggcgctaaa caggagggaa
    35341 agaagagtat gctttcaacg gcttagctac tcgtttaaag gattaatcat gaagttgaat
    35401 tttaagggat tttttaaggc tgccggttta ttcccactgg cgctgatgct ttcaggctgt
    35461 atctcgtatg ctctggtttc ccataccgca aagggttgtt caggaaagta tcaatcgcag
    35521 tcagacacca tcactgggct atcgcaggca aaagatagta atggaacaaa aggctatgtt
    35581 tttgtagggg aatcgctgga ttaccttatc actgatggtg ccgatgacat cgttaagatg
    35641 ctcaatgatc cagcacttaa ccggcacaat attcaggttg ccgatgacgc aagatttgtt
    35701 ttaaatgcgg ggaaaaagaa atttaccggc acaatatcgc tttactacta ctggaataac
    35761 gaagaagaaa aggcactggc aacgcattat ggttttgcct gtggtgttca acactgtacc
    35821 aggtcactgg aaaacctaaa aggcacaatc catgagaaaa ataaaaacat ggattactca
    35881 aaggtgatgg cgttctacca tccatttaaa gtgcgatttt atgaatacta ttcacccaga
    35941 ggcattccgg atggtgtttc cgcagcatta ctgccagtga ctgttacact ggacatcatt
    36001 actgcaccgc tgcaatttct ggttgtatat gcagtaaacc aataatcagt aagcgggcaa
    36061 acgcgtttat gctgtttgcc cgcccacaga ttaattcagc acatacttct caatagcaaa
    36121 cgccacgcca tcttcaaggt tggattttgt gacaaagttc gccacttctt tcaccgaagg
    36181 aatagcgtta tccatcgcca caccgacgcc tgcatattca atcattgcga tgtcgttttc
    36241 ctgatcgcca atcgccatga tttcttccgg tttaatacct aacacgtcgg ccagtgattt
    36301 cacacccgta cctttgttaa cgcgtttatc gaggatttcg aggaagtacg gcgcactttt
    36361 cagcacggta tatttctctt tcacttcctg cggaatacgc gcgatagcct ggtcgaggat
    36421 ggcgggttca tcaatcatca tcactttcag gaactgggta ttggggtcca ttttctccgc
    36481 ttcgcagaac accagcggaa ttgtggcaac gaaggattca tgcaccgtgt agtagctgat
    36541 atcgcggtta gcggtgtaca gcgtggtgcg gtccagggcg tggaaatgag aaccgacttc
    36601 gcgagagagt ttttccagga aacgatagtc gtcatagctg agagcggttt gcgccacggt
    36661 gctaccatca gcggccttct gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg
    36721 ctgttccata tgcagctctt tcaggtagtt gtgcacacct gcatacggac gacccgtcgt
    36781 tagcacgaca ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga
    36841 aatggtgtga tcgggcagca gaagggtgcc atccatatcg atagcaatga gtttgatagc
    36901 catgagttcc caaggtagat tagttcctga cccatgctaa cgcgattccg ctcaaaaatc
    36961 agtacaacac ccgagggaaa aggggatgca acgcgggttg catgctcccc atttgcttag
    37021 cggaagagtt tccctttcag cagttccatg cctgcggaaa gcagatcgtt attggcttgt
    37081 gctgacactt caccttgcgg tgagagcgca tcaataatct tcggcaattg ctctgccagt
    37141 aaactggaag ccgtactggt atccacgcca agtttttgcc cgagatcgga caccgcattt
    37201 gtgccgagcg ccgactccag ttgctcgccg ctaactgatt gatttctctg ttgattactc
    37261 aaccaggttg agagaatggc ccctaagccg ccactttgca gtttttccag cagcacctga
    37321 atgccaccct gctcctcaac ccaacttaaa atcgcctgat atttccccgc atcgcctttc
    37381 agaaaggcac cgacaacttc atcaaaaagc cccatgataa tcacctgtaa agcgttacgt
    37441 gttgacccaa aaagtataga tctgcgggat gataattgcg gattgcagaa ataaaaaggg
    37501 cggagatgat ctccgccctt ttcttatagc ttcttgccgg atgcggcgtg aacgccttat
    37561 ccggcctaca aaatcatgca aaatcaatat attgcaggag tctcgtagga ctaataagcg
    37621 tagcgcatca ggcacgctcg catggttaac gccattaaat atcaatattc gccgctttca
    37681 gggcgttctc ttcaataaac gcacgacgcg gttctacggc gtcgcccatc agcgtggtga
    37741 acaactggtc agcggcaatc gcatccttaa cggtaacgcg cagcatacga cggctttccg
    37801 ggtccatggt ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct
    37861 ggatggagag gccacgacgg gactctttca ccagccagtc cagcgcctgc tcgaagctgg
    37921 ctaccggctg acgacgctcg ccacgttcga taaacgcatc ttcttccagc aagccacgca
    37981 gtttctcacc cagcgtgcag atacgacgat attcgccacc ggtgataaac tcgtgatcca
    38041 gcggatagtc agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt
    38101 gttcggcatt ggtgtggaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca
    38161 gttcgctgac cagcgcgttc acccagcggg taacggtctg ctcatcagaa aggtcagctt
    38221 ccgtcaacgt cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca
    38281 tgcggttgat cattttctgc gtcgcgttgt actcagatac cagtttctct aacgcttcgc
    38341 cagccaatgc cggtgcactg gcgttggtgt gcagcgttgc gccgtccagc gcgatagaga
    38401 tctggtactg atccatcgct tcgtcgtctt taatgtactg ttcctgcttg cctttcttca
    38461 ctttgtacag cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct
    38521 gacgatagaa gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg
    38581 tcatgatgat gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac
    38641 cacagccaag cgcggtgata agcgtcgcca cttcctgaga agagagcatc ttatcgaagc
    38701 gcgctttctc gacgttaagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt
    38761 tacgcccctg cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcggaaa
    38821 gcgccggatc gcgttcctgg cagtctgcca gtttgcccgg caggccagct aagtcgagcg
    38881 cacctttacg gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat
    38941 cgataatttt gccaaccacg attttcgcat cagttgggtt ttccagcagg tattccgcca
    39001 gcagttcgtt catctgctgt tcaaccgccg atttcacctc agaagaaacc agtttgtctt
    39061 tggtctggga ggagaatttc gggtccggca cttttacgga aacgaccgca atcaggcctt
    39121 cacgcgcatc gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca
    39181 tgtaggcatt cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt
    39241 cacgctgcgg aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact
    39301 gcagcgccac ttcaacgcca ataccgtctt tttcggtgga gaagtagaag atatttgggt
    39361 ggatcggcgt tttgttcttg ttcagatatt caacgaacgc tttgatgccg ccttcatagt
    39421 ggaagtggtc ttctttgccg tcacgcttgt cgcgcagacg aatggaaacg ccggagttga
    39481 ggaacgacaa ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg
    39541 tgaaggtttc gaggctgggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg
    39601 taaccgccag cggagcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct
    39661 cgcgctgaat aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc
    39721 cgtgcagacc gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa
    39781 cggtcatgat cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc
    39841 cgcgcccgtc atcctgtaca gagacagagt tatcggcgtg aatggtgacg ataatttctt
    39901 tacagtgacc cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt
    39961 gcagaccggt gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat
    40021 ccagcccttt caggactttg atactggagg agtcataaga attcgacatc aacgtttctc
    40081 gctcatttat acttgggtta atctgttatt ttaccctttt ccacggtaaa catcttcgaa
    40141 ttttcgtccg acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat
    40201 tgcgtcgctt ttaagcggct ggcaagcaac ccgcgacgct catcatcaag ctcagaggca
    40261 aaatcatcta tcaggtagag acaccgccgc ccgctttcac gagtgaggaa ctctccttgc
    40321 gccagacgta aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc
    40381 ggcgcaccgt cggcgcgaat gcgtaagtcc gctttatgcg ggccatgcgc ggtgtaggtt
    40441 agctggcgat cgcgttcaaa attacgttcc agcacctcag cgtactctgt ctctttctcc
    40501 cagccgcgct ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaggtatca
    40561 tccatatcgg ccgcgatacc ggcgctatac tccgcgcgcc aggtgctgat ttgctccgcc
    40621 agcgggatca gttctttatc ccacgggcgt agctgttcgt aacgagtcac ctggcgcagc
    40681 gccgcattgc gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccaggt
    40741 tcgttgtgaa agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt
    40801 aaagtaaacc cttctggcgt tatcaactgc atcggcatca ggtgcgccag ttccgcgact
    40861 ttatgcccgt ccgtaccgtc aatacggact ttgctgtcgc cctgtttgtc tttggttaag
    40921 ccaatcgccg tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa tgcctcctgc
    40981 tcatggcgaa tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta
    41041 tagatggctt ccagcacgct ggttttgcca ctgccgttgg cacctaccag aaagttaaag
    41101 ccgggagata aagcgagatc cgcggtttca atattgcgga aatcgcggat caacaagcgg
    41161 gtgagggaca ttacagtctc attggcatga caacataggc agccgactgt gatgcggcat
    41221 cttcaatctg cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca
    41281 gcgcgttcag aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc
    41341 tataggtaac gtcgaggatc tcttccgctt cttcctgttc cgggttgttg gcagtaattt
    41401 tcagctggtt ttcgctgaca tacaggcgca caccgcggaa tttctcgtta gagagaatcg
    41461 ccgcgcgagc aaacgcctgc ttgagcagat cgcagcctgc ttccagatgt ttgtccgggt
    41521 tcttcggcag aacgcgacgg taatccggga agcgaccatc caccagtttc gaggtgaaaa
    41581 taaagtcgcc aacgtgggca cgaatgttat tgctgccaat ctgcacacgc agcagattgt
    41641 cgccgccgtc gagcatacgc atcagttcaa tcacgccttt acgcggtacg atcaccgaat
    41701 ggcttggcaa agattgacca attggcattg aacagaccgc cagacggtgg ccgtcggttg
    41761 ccacggtgcg cagttcttca ccttcggttt caaacagcat accgtttaag taatagcgaa
    41821 catcctgatg cgccatcgaa aactgggttg cttcaatcag acgcttcatc gtcgcctgcg
    41881 gcagggtaaa ttcgacttcg ctctgccagt catcgaggtt cgggaaatcc gccgctggca
    41941 gggtagacag cgaaaaacgg ctacgcccgg agcgcaccag catccgttca ccttccagct
    42001 gcacggcaat ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg
    42061 gaacggtcgt cgctcctggc tcgtgtggct gaaccagcgc aacgcgtgcc accatttcca
    42121 tctcgagatc agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac
    42181 cgagaatcgg tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta
    42241 ataaatgctc acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa
    42301 tttgaaaaat cttctttgat atcgtggctc tcttcacgca actgctcgat cttacggcag
    42361 gcatgaagca ccgtcgtgtg gtcacgccca ccaaacgcat cgccaatctc tggcagactg
    42421 tggttagtca gctctttcgc tagcgccatc gccatctggc gcggacgcgc caccgagcgg
    42481 gatcgacgct tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc
    42541 tgaatattgt cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca
    42601 cgcacgaagt cgatggtgat cgcccgtccg gtaaagttgg cgttagcaat aacgcggttc
    42661 agcgcccctt ccagctcacg tacgttagat cgtagacgct tggcgataaa gaacgccact
    42721 tcacccggca aacgaatgtc attttcgtcg gcctttttca tcaggatcgc cacacgggtt
    42781 tccagctctg gcggttcgat cgccacagtc agtccccaac cgaagcggga tttcaaacga
    42841 tcctcaacgc cgttgatctc tttcggatag cgatccgagg tgagaatgat ctgttgatta
    42901 ccttccagca gggcgttgaa ggtgtggaaa aactcttcct gagatcgttc tttattagca
    42961 aaaaactgaa tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct
    43021 tcgatcgcgt tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata
    43081 taaaccactt tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg
    43141 tgagttttac ccagacccgt gccgccataa aggaacaacg ggttataggc accgccaggg
    43201 ttatccgcca cctggcaagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag
    43261 ttatcaaacg tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggggccggg
    43321 acgttatccc aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc
    43381 tgtgcagggg ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg
    43441 gtgccgactt caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca
    43501 ttgatattat taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac
    43561 agggccagcg tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct
    43621 gtggctggta actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg
    43681 gcggactcca ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga
    43741 cgtacgtcga aaagaccctg cttgagggtg acgcacgaac cgctgtctgc ggttatatgc
    43801 ccgatcaaga tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga
    43861 tcttctgttt ctcacagatt ttccctattt atccacagga ctttccagaa ctcgctaagt
    43921 gtaaacgatc ctgccgcgag gcgggcacga tttacgccgc atattggaaa atttaatgac
    43981 catagacaaa aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac
    44041 ccatcagccc gtataatcct ccacccggcg cgccatgctg gtttccactg gtgtgaggtc
    44101 gtacattttc cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact
    44161 ccggtgtgta caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc
    44221 gtttcatttt tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa
    44281 tcgccatgaa acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc
    44341 gtgctcgtat ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc
    44401 gcgctcgtct gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt
    44461 cccagggagt tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa
    44521 cgggctggca cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt
    44581 atcggtctta cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa
    44641 cgcctgacgc gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg
    44701 gtggtggcga aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa
    44761 aaattatggc gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct
    44821 atcaacgcct gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa
    44881 gctacggaat tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga
    44941 aacgcgtatt aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac
    45001 catttgatac cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt
    45061 gctgttcgtg tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca
    45121 ggcccaacag accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt
    45181 accggccagt ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat
    45241 caacacccgt ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa
    45301 ctctacccag ccgttccagc tgttggaaac ttcaccgcag tttatttatc aggcacagag
    45361 cggtctgacc ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa
    45421 cgttgaaaaa gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac
    45481 gtataccgac gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta
    45541 cgctgtcaac gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctcctc
    45601 gtttggtcag ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa
    45661 cttcgcactg cacaccttcc gtggcgcggc gtactccacg cctgacgaga agtatgagaa
    45721 atacaagttc gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg
    45781 ggtggcgatg ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg acggtaccaa
    45841 caacttctat accgctaatc tgggtaacgg catcgtcgct atcggctata aatctcagcc
    45901 ggtactggtt cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga
    45961 aatccaggac aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg
    46021 gttgtggttc atctctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg
    46081 taactggggc ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct
    46141 gaccaaagcg cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc
    46201 aatgcgtgag cgtctgggcg atgacaaaca gcgtatcagt caggaaatga tggcgctgta
    46261 caaagctgag aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat
    46321 cttcctggcg ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc
    46381 actgtggatc cacgacctgt cggcacagga cccgtactac atcctgccga tcctgatggg
    46441 cgtaacgatg ttcttcattc agaagatgtc gccgaccact gtgaccgacc cgatgcagca
    46501 gaagatcatg acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg
    46561 tctggtactg tactatatcg tcagcaacct ggtaaccatt attcagcagc agctgattta
    46621 ccgtggtctg gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga
    46681 gttttcgcta aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa
    46741 gcaccgcgca tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt
    46801 agcccaggcc acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa
    46861 agcccgtgaa gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta
    46921 tcttccgttt aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc
    46981 tggcccgaac tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt
    47041 gatcctcgac ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc
    47101 tggtgagttt tccgaacgcg cgtttcttaa cgataaactt gacttagccc aggccgaggc
    47161 gattgccgat cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct
    47221 gcaaggcgca ttctccgcac gggttaatca tctggtagaa gccctcaccc acttgcgcat
    47281 ttacgtcgaa gcggcaattg atttccctga tgaagagatc gatttcctct ccgacggcaa
    47341 aattgaagcc cagcttaatg acgtgatggc cgatctcgat gcagtgcgtg ctgaagcacg
    47401 tcagggtagt ttgttgcgcg aagggatgaa agtagtgatt gccggacgtc ctaatgccgg
    47461 taaatcgagc ctgttaaacg cgctggcggg ccgtgaagcg gcaatcgtaa ccgatatcgc
    47521 cggaaccacg cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc cgctgcatat
    47581 catcgacact gccgggctac gtgaggccag tgacgaagtg gaacgtattg gtatcgagcg
    47641 cgcgtggcag gaaattgaac aggccgaccg cgtgctgttt atggtcgatg gcaccacgac
    47701 agacgccgtt gatccggcag agatctggcc ggaatttatc gcccgtctgc ccgcgaaact
    47761 gccgatcacc gtggtacgca ataaagccga tatcaccggc gaaacgctgg gaatgagtga
    47821 agtgaacggt cacgcgttaa ttcgtctctc ggcaaggact ggtgaaggcg tggacgtgct
    47881 gcgtaaccat ctcaaacaga gcatgggctt tgacaccaat atggaaggcg gcttcctggc
    47941 gcgccgtcgc cacttacagg cgctggaaca ggcagcggag catttgcagc aaggtaaagc
    48001 gcaactgttg ggagcctggg caggtgaact gctggcggaa gagttgcgcc tggcacagca
    48061 gaacttaagc gaaatcaccg gggaatttac ttcagacgac ctgctggggc ggattttctc
    48121 cagcttctgt attggtaagt aaccgcgctt acgaagccgt attctgactg tcagatgcgg
    48181 cttcgcttca ttgttagcac tcctgttatg cctcaaccct tttttaaaca ttaaaattct
    48241 tacgtaattt ataatcttta aaaaaagcat ttaatactgc tccccgaacg attgtgattc
    48301 gattcacatt taaacaattt cagaatagac aaaaatt
//
LOCUS       DAOFMX010000023        48071 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.023, whole genome shotgun sequence.
ACCESSION   DAOFMX010000023 DAOFMX010000000
VERSION     DAOFMX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.023"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..213)
                     /locus_tag="QBA20_001487"
     CDS             complement(<1..213)
                     /locus_tag="QBA20_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HDQ6292074.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSS"
     gene            708..1262
                     /gene="pinE"
                     /locus_tag="QBA20_001488"
                     /pseudo
     CDS             708..1262
                     /gene="pinE"
                     /locus_tag="QBA20_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(1601..2449)
                     /locus_tag="QBA20_001489"
     CDS             complement(1601..2449)
                     /locus_tag="QBA20_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDQ6292075.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            2658..3293
                     /gene="yfhb"
                     /locus_tag="QBA20_001490"
     CDS             2658..3293
                     /gene="yfhb"
                     /locus_tag="QBA20_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDQ6292076.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            3318..3854
                     /gene="tadA"
                     /locus_tag="QBA20_001491"
     CDS             3318..3854
                     /gene="tadA"
                     /locus_tag="QBA20_001491"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDQ6292077.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(3851..5407)
                     /gene="mltF"
                     /locus_tag="QBA20_001492"
     CDS             complement(3851..5407)
                     /gene="mltF"
                     /locus_tag="QBA20_001492"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDQ6292078.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            5666..9553
                     /gene="purL"
                     /locus_tag="QBA20_001493"
                     /gene_synonym="purI"
     CDS             5666..9553
                     /gene="purL"
                     /locus_tag="QBA20_001493"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDQ6292079.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10129..11556
                     /gene="qseE"
                     /locus_tag="QBA20_001494"
     CDS             10129..11556
                     /gene="qseE"
                     /locus_tag="QBA20_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDQ6292080.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            11721..12434
                     /gene="qseG"
                     /locus_tag="QBA20_001495"
     CDS             11721..12434
                     /gene="qseG"
                     /locus_tag="QBA20_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDQ6292081.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            12424..13758
                     /gene="glrR"
                     /locus_tag="QBA20_001496"
     CDS             12424..13758
                     /gene="glrR"
                     /locus_tag="QBA20_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDQ6292082.1"
                     /translation="MSHKPAHLLLVDDDLGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            13819..14157
                     /gene="glnB"
                     /locus_tag="QBA20_001497"
     CDS             13819..14157
                     /gene="glnB"
                     /locus_tag="QBA20_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDQ6292083.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(14202..15392)
                     /gene="hmpA"
                     /locus_tag="QBA20_001498"
     CDS             complement(14202..15392)
                     /gene="hmpA"
                     /locus_tag="QBA20_001498"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDQ6292084.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGLVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            15719..16972
                     /gene="glyA"
                     /locus_tag="QBA20_001499"
     CDS             15719..16972
                     /gene="glyA"
                     /locus_tag="QBA20_001499"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDQ6292085.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(17170..18363)
                     /locus_tag="QBA20_001500"
     CDS             complement(17170..18363)
                     /locus_tag="QBA20_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDQ6292086.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            18481..18654
                     /locus_tag="QBA20_001501"
     CDS             18481..18654
                     /locus_tag="QBA20_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292087.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            18661..21762
                     /locus_tag="QBA20_001502"
     CDS             18661..21762
                     /locus_tag="QBA20_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDQ6292088.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKCDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            21859..22842
                     /locus_tag="QBA20_001503"
     CDS             21859..22842
                     /locus_tag="QBA20_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDQ6292089.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            22865..24376
                     /locus_tag="QBA20_001504"
     CDS             22865..24376
                     /locus_tag="QBA20_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDQ6292090.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            24401..25399
                     /locus_tag="QBA20_001505"
     CDS             24401..25399
                     /locus_tag="QBA20_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6292091.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            25432..26526
                     /locus_tag="QBA20_001506"
     CDS             25432..26526
                     /locus_tag="QBA20_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDQ6292092.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            26538..27410
                     /locus_tag="QBA20_001507"
     CDS             26538..27410
                     /locus_tag="QBA20_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDQ6292093.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(27458..27880)
                     /locus_tag="QBA20_001508"
     CDS             complement(27458..27880)
                     /locus_tag="QBA20_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDQ6292094.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(27977..29184)
                     /locus_tag="QBA20_001509"
                     /pseudo
     CDS             complement(27977..29184)
                     /locus_tag="QBA20_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(29194..30006)
                     /gene="hcaB"
                     /locus_tag="QBA20_001510"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(29194..30006)
                     /gene="hcaB"
                     /locus_tag="QBA20_001510"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDQ6292095.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(30003..30323)
                     /locus_tag="QBA20_001511"
     CDS             complement(30003..30323)
                     /locus_tag="QBA20_001511"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDQ6292096.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(30323..30841)
                     /locus_tag="QBA20_001512"
     CDS             complement(30323..30841)
                     /locus_tag="QBA20_001512"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDQ6292097.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(30838..32199)
                     /locus_tag="QBA20_001513"
     CDS             complement(30838..32199)
                     /locus_tag="QBA20_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDQ6292098.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            32335..33225
                     /gene="hcaR"
                     /locus_tag="QBA20_001514"
     CDS             32335..33225
                     /gene="hcaR"
                     /locus_tag="QBA20_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDQ6292099.1"
                     /translation="MELRHLRYFIAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            33385..34524
                     /locus_tag="QBA20_001515"
     CDS             33385..34524
                     /locus_tag="QBA20_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDQ6292100.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(34516..35796)
                     /gene="csiE"
                     /locus_tag="QBA20_001516"
     CDS             complement(34516..35796)
                     /gene="csiE"
                     /locus_tag="QBA20_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDQ6292101.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(35987..36841)
                     /locus_tag="QBA20_001517"
     CDS             complement(35987..36841)
                     /locus_tag="QBA20_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ6292102.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(36986..37789)
                     /gene="suhB"
                     /locus_tag="QBA20_001518"
     CDS             complement(36986..37789)
                     /gene="suhB"
                     /locus_tag="QBA20_001518"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDQ6292103.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            37908..38648
                     /gene="trmJ"
                     /locus_tag="QBA20_001519"
     CDS             37908..38648
                     /gene="trmJ"
                     /locus_tag="QBA20_001519"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDQ6292104.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            38918..39406
                     /gene="iscR"
                     /locus_tag="QBA20_001520"
     CDS             38918..39406
                     /gene="iscR"
                     /locus_tag="QBA20_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDQ6292105.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDVIDVKL
                     RA"
     gene            39518..40732
                     /gene="iscS"
                     /locus_tag="QBA20_001521"
     CDS             39518..40732
                     /gene="iscS"
                     /locus_tag="QBA20_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDQ6292106.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            40760..41146
                     /gene="iscU"
                     /locus_tag="QBA20_001522"
     CDS             40760..41146
                     /gene="iscU"
                     /locus_tag="QBA20_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDQ6292107.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            41163..41486
                     /gene="iscA"
                     /locus_tag="QBA20_001523"
     CDS             41163..41486
                     /gene="iscA"
                     /locus_tag="QBA20_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDQ6292108.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            41582..42097
                     /gene="hscB"
                     /locus_tag="QBA20_001524"
     CDS             41582..42097
                     /gene="hscB"
                     /locus_tag="QBA20_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDQ6292109.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            42114..43964
                     /gene="hscA"
                     /locus_tag="QBA20_001525"
     CDS             42114..43964
                     /gene="hscA"
                     /locus_tag="QBA20_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDQ6292110.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            43966..44301
                     /gene="fdx"
                     /locus_tag="QBA20_001526"
     CDS             43966..44301
                     /gene="fdx"
                     /locus_tag="QBA20_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDQ6292111.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            44313..44513
                     /gene="iscX"
                     /locus_tag="QBA20_001527"
     CDS             44313..44513
                     /gene="iscX"
                     /locus_tag="QBA20_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDQ6292112.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            44572..45855
                     /gene="pepB"
                     /locus_tag="QBA20_001528"
     CDS             44572..45855
                     /gene="pepB"
                     /locus_tag="QBA20_001528"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDQ6292113.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            45997..46773
                     /gene="sseB"
                     /locus_tag="QBA20_001529"
     CDS             45997..46773
                     /gene="sseB"
                     /locus_tag="QBA20_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDQ6292114.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(47592..>48071)
                     /gene="sseA"
                     /locus_tag="QBA20_001530"
     CDS             complement(47592..>48071)
                     /gene="sseA"
                     /locus_tag="QBA20_001530"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDQ6292115.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT        11396 a        12037 c        13100 g        11538 t
ORIGIN      
        1 cgaggaaagc gccaaggctc gcaggtccag tttatgcccc ttagaggtaa gacatgcacg
       61 aagtcttctt accgcctcag tacgctgttc ttccggtaac ccctgtttct cccaatcatc
      121 ccattcggac aaataatggt tgggggatgt aattgtgctg atatgtggaa ataaacagga
      181 tgaaatattt ctgagcatat taatttctcg catatggata tggaattaga ttgtgaataa
      241 attgtattta atgtatctct cctaatgagg agggtgtttc agaatatttg ccataaggga
      301 atgagtgtta tatcatgctt tctaccttgg taagtatatt tgcttttttc gtatatttgg
      361 ttcatctttt tttgatgttt tacatattca caatgcagag gatttatacc agaagacatt
      421 agtcttgagt aagatactac aacatctcca actattttag ccagcctgac tacttgactt
      481 ggtaatattt gttgctaata atcatgtata aaataagctt atatacatag taacttcagc
      541 aaaggattta gtaatgatcc aaatcatatc tcaatgtact gtttttttct ttaaaaaaac
      601 agtacattct attttataat gactgtgtat tcccatggaa ggagatcacc caattctccc
      661 ccccccccga cttttgacgc agtgtcttgt taacaggggg atttaccatg cttattggct
      721 atgtacgcgt atcaacaaat gaccagaata ccgatctaca acgtaatgcg ttgaactgtg
      781 caggatgcga gctgattttt gaagacaaga taagtggtac aaagtccgaa agaccggggc
      841 tgaaaaaact gctcaagaca ttatcggcag gcgacacact ggttgtctgg aagctggatc
      901 ggctggggcg cagtatgcgg catcttgtcg tgctggtgga ggagttgcgc gaacgaggca
      961 tcaactttcg tagtctgact gattcaattg ataccagcac accaatggga cgctttttct
     1021 tccatgtgat gggtgccctg gctgaaatgg aacgtgaact gattgttgaa cgaacaaaag
     1081 caggactgga agctgctcgc gcacagggac gaattggcgg tcgccgtccc aaactttcac
     1141 cagaataatg ggcgcaggcc gggcgactaa ttgcatcagg agtttctcgc cagaaggtgg
     1201 cgatcatcta tgatgttggc gaatcgactt tgtataagag gtttccagcc agggttatat
     1261 aaaaccgcag acacggcgta tacaagaacg tgctacggtt gactgaaaag ttaatgttgt
     1321 atgtcacagg ccttttgcgc cgtaaagccc cgtcctttag ggcgcggagg acgttaaaga
     1381 aagtggtgtt ctttgtgttg ctgaacttat gcaaaatttt tttgcaccat aaatccaaca
     1441 acacattcaa taacacattg ctgtatcact atgaaaataa atgtgttttt atttgatttc
     1501 acacatatca caattgatgc attttttagt gattaacagc gccataagga aactctcaaa
     1561 acaacacaaa ccgggcgggc attatacgcg cattctttac tcagaccagt tttttcacca
     1621 gcgcttcact atgacgaata cgttctggtg ccagttccag atcctgctga atcagcgcaa
     1681 tgaacagcaa atctgttaac attccctgag cgtgacaagc agagattgaa gcactgtttg
     1741 tcgcttgttc ttcggcaatg gtatacaggc aatgagaagc acgctgttgc agggcattcg
     1801 gagtaaagcc ggtaatcgcc agcacttttc cgcccactcg cagcatctca tctgccgcca
     1861 ggtttaactc gcgtcgtaca ccggtgtagg aaatggctaa taacagatcg tcaggggacg
     1921 acgcctgtac tgttgcgagt agcgcatgca tatcgcgcag tgcggcagca ttgaagccaa
     1981 tcttcatcag cttccaggca aagttttgcg ccaccagacc cgaagcgcca ataccggtca
     2041 gaattatccg ccgcgcagag cgcaacattg ttacgcattc atgcagtttc tcttcactat
     2101 taacgtttag cgttgcgtac atagcggcag tattttcttt aatcagtttt tcgccgacca
     2161 gccgtaacgg atcatcaccg cggatttggt tatgaatggg cacggagggc gattccggct
     2221 ggcttgccag cgcttcactc aacgccaact taagcgccgg aaaaccttta tagccgagtt
     2281 tttgcgcgaa cttcacgacg ctggactgac tgactccggc ttcgttggcc agttgctgag
     2341 agcttaaatg gcgcgccgta tcaggttgta gcagcagata atccgccagt tttttatcac
     2401 tctgggcaag cccttggtaa cgctgacgga tacgaagtaa gccgttcata tctttcctcg
     2461 cgatgaaata gtggcgacaa caggcaatat gagacacacg cgagtgaatt ttatattcca
     2521 ttgccgctaa ttgccatgaa ttaaaaattc cagttagcca tgatgcggtc gattgttgcc
     2581 ggaaaagtga tgatctatct gttaggctat gtagtagcga aaattaatca ggatgtttca
     2641 gtccagagga gtatggtttg gcaactcacg agcgtcgtgt ggtgtttttt gacttagatg
     2701 gaacattgca tcagcaggat atgttcggca gttttctgcg ctatttactg cgtcgccaac
     2761 cgctgaatgc gttacttgtc ctgccgttgt taccgattat agccattgcg ttattgataa
     2821 aaggtcgtgc ggcacgctgg ccgatgagtc tgcttctgtg ggggtgcact tttggtcaca
     2881 gcgaagcacg tttacagacg ttgcaggccg atttcgtgcg ctggtttcgc gacaatgtta
     2941 ccgcctttcc gctggttcag gagcgattaa ccacctacct gttaagttcc gatgctgata
     3001 tctggttgat taccggctct ccgcagccgc tggttgaagc ggtttatttc gatacgccct
     3061 ggctgccgcg ggttaatctt atcgccagcc aaattcagcg tggctatggt ggttgggtat
     3121 tgacgatgcg ttgtctggga catgaaaagg tcgcacaact ggagcgcaaa atcggcacgc
     3181 cgctgcggct gtacagtggc tatagcgaca gtaatcagga caatccgctg ctttatttct
     3241 gtcagcatcg ttggcgagta accccgcgcg gtgaactcca gcaactggaa tagagtaaag
     3301 catagcgtcc gtgtataatg cgccgcgctt ttataaccgg agttttcttt ttgtctgaag
     3361 tcgaatttag ccacgaatat tggatgcgtc acgcgctgac gctggcgaaa cgtgcctggg
     3421 atgagcggga agtgccggtc ggcgcggtat tagtgcataa caatcgggta atcggcgaag
     3481 gctggaaccg cccgattggt cgccatgatc ccaccgcaca tgcagaaatc atggccctgc
     3541 ggcagggtgg tctggtgatg caaaattatc gtctgatcga cgccacgttg tatgtcacgc
     3601 ttgaaccatg tgtaatgtgt gccggagcga tgatccacag tcgcattggt cgcgtggtct
     3661 ttggtgcgcg tgacgcgaaa actggcgctg cgggatcttt aatggatgtg ctgcatcatc
     3721 cgggtatgaa tcaccgagtg gaaattacgg aaggaatact ggcggatgag tgcgcggcgt
     3781 tgctcagtga cttctttcgc atgcgccgcc aggaaattaa agcgcagaaa aaagcgcaat
     3841 cctcgacgga ttaattttgt ttctcttcac tccctttcct ggaaaacagc agagagggag
     3901 aatgggtcaa atagtttgat gacgactggg aaagaaacga gagaaaagga aatttctctt
     3961 tgcccaactc cgtaggcgat accgccggat aatcctgcgc cagttgcatc gccgcttctg
     4021 tagcctgctt ctctttctct tgcagataac ccaccaggct aatctgatac ttacgaatat
     4081 tttcgacata agcgtaggct tcatgcccac gagcgtagcc gtaagtcagc ttgctgtaat
     4141 agggtttctg gctaagtaaa ggcagacgct gttttacgtc agcccaactg tcaggattcc
     4201 ctttggtttt tgccgtcagg gcgcgggcat ccagcatatg cgcatagccc atattgtacg
     4261 cagcgagggc aaaccagatc cgttcgttct ccggcacact ttccggcact ttactcatca
     4321 tatcctgcaa ataacgcacg ccaccgctga tgctctgttc ggcatcggta cgatccgtaa
     4381 tgccgaggct ttgcgcggta tttttggtta acatcatcat gccgcgcaca cccgtcggtg
     4441 aagtggcctg tgcatcccag tgcgattcct gataagcaat agcggccagc aaacgccagt
     4501 caatttcttc ggcgtatttc tcaaacaggg gctttaactg cggcagtacc gcatcgacgg
     4561 cgcgtaaaaa tgtgcgcgta tcgacgtaat caaaatcatc gccatgcccc aggtatttct
     4621 cttcaatgcg tgccagcgta ccgtcttcat tcatttcgtt gaagaagtcg agcagggcgg
     4681 cggaaagggt attatcgcca tctaacgggc taaaccaagt caccggttgt tcatcggtga
     4741 tatcgagcgc tacggcgagc tccgggtgaa cgcgctgaaa caggctgatg gcgacagaat
     4801 cagcaatggt gtaatcgagt tttccttcga tgacatcttc cattaattcc gcagagcctt
     4861 ttttgtcgtc taccttccag cttaattccg ggaattttgt ttctttcagg gtctggagat
     4921 cgttaaccac cacatgaccc ggtgcaacgg tgagttgctc cgccgtcagg ttgcccagcg
     4981 tacgtgggcg atactgaccc actttataaa ccagttgttg tgacacggaa taataggtag
     5041 ggccaggctg ataatttttt acccgctcac tgttatagac cagtcctgcc gccagcaggt
     5101 cggcgttacc attatcaagg tcgtcaaaca gctggctgat attctgccgc acggtcactt
     5161 tcagttttac gccgaggtaa tcggcaaact gtttcgccag ttcgtaatct aggccaaaag
     5221 gtttcccgtt gatttcgtta taagtcaggg gagtatgaat ggtgctcaca cgcaactctc
     5281 cccgcgcttg aatggcggcg atacggttgt cggctttacc aaaccaggga atggatggcc
     5341 agagagcgac cgcgagcagc agtgccagaa tgccgatgaa cagataatta atctttaatt
     5401 ttttcaatta gttaattctc tgtgtcgtgc gcgtcccagc ttgaaaaaac gtaataatag
     5461 tgaaaggttt actcataaat gagcggcatt ttgcgtaaac ctgcgccaga tggcaactta
     5521 ttacagccat tggcggcacg cgttgctaat tcacgatggt gattttattt ccacgcaaac
     5581 ggtttcgtca gcgcatcaga ttctttataa tgacgcccgt ttcccccccc ttgggtacac
     5641 cgaaagctta gaagacgaga gacttatgat ggaaattctg cgtggttcgc ctgcactgtc
     5701 ggcattccga atcaacaaac tgctggcacg ttttcaggct gccaggctcc cggttcacaa
     5761 tatttacgcc gagtatgtcc attttgctga cctcaatgcg tcgttaaacg atgatgagca
     5821 cgcacaactt gaacgcctgc tgaaatatgg tccggcactc gccagccacg ccccgcaagg
     5881 caaactcctg ctggtgaccc cgcgtcctgg caccatctct ccctggtctt cgaaagcgac
     5941 cgatattgcc cataactgcg ggctacaaca ggtaaaccgc cttgagcgcg gcgttgctta
     6001 ctatatagaa gccggtacgc tgaccaatga acaatggcag caggttaccg ctgaactgca
     6061 cgaccgcatg atggaaacgg tcttttttga tttagatgat gcagagcagt tgtttgccca
     6121 ccatcaaccg actccggtca caagcgttga tttgctgggg cagggccgtc aggcgctgat
     6181 cgacgctaac ctgcgtcttg gcttggctct ggcggaagat gaaattgact atctgcagga
     6241 tgctttcaca aagcttggtc gtaacccgaa cgacatcgag ctgtatatgt tcgctcaggc
     6301 gaactctgag cactgccgcc acaaaatttt taacgccgac tggattatcg acggtgaaca
     6361 acagccgaaa tcgctgttca ggatgatcaa aaatactttc gaaaccacgc cagatcacgt
     6421 tctctctgct tataaagata acgccgccgt aatggaaggt tctgaagtgg gccgctactt
     6481 tgctgaccac gaaacgggcc gctacaattt ccatcaggaa ccggcgcaca tcctgatgaa
     6541 agtcgaaact cacaaccacc cgacggcgat ttctccgtgg ccgggcgcgg cgaccggttc
     6601 cggcggtgaa atccgcgatg aaggtgccac cgggcgcggc gcgaagccga aagctggttt
     6661 ggttggtttc tccgtatcca acctgcgaat tcctggcttc gaacagccgt gggaagaaga
     6721 tttcggtaag cctgagcgca ttgttaccgc gctggacatc atgaccgaag gcccgctggg
     6781 cggtgcggcg tttaacaacg aatttggtcg tccggcactg aacggctact tccgtactta
     6841 tgaagaaaaa gtgaacagcc acaatggcga agagctgcgc ggttatcaca aaccgatcat
     6901 gctggcgggc gggatcggca acattcgcgc cgatcacgta caaaaaggcg agatcaacgt
     6961 cggtgcgaag ctggtcgttc tcggcggccc ggcaatgaac atcggtcttg gcggcggcgc
     7021 ggcgtcttcc atggcgtctg gtcagtctga tgccgacctc gactttgctt ccgtacagcg
     7081 cgacaacccg gagatggagc gtcgttgcca ggaagtgatc gaccgttgct ggcagcttgg
     7141 tgatgctaac ccaattctgt ttatccacga cgtcggtgcg ggcggtcttt ctaacgcgat
     7201 gccggagctg gtgagcgacg gcggacgcgg cggtaaattt gaactgcgcg atattctaag
     7261 cgacgaaccg ggcatgagcc cgctggaaat ctggtgtaac gaatcccagg aacgctacgt
     7321 gctggcggtt gctgccgatc aattaccgct gtttgacgaa ctgtgtaagc gtgagcgcgc
     7381 accctacgcg gtgattggtg aagcgaccga agaactgcat ctttctctgc acgatcgtca
     7441 ttttgataat cagccgatcg atctgccgct ggacgtcctg cttggtaaaa cgccgaagat
     7501 gacccgcgat gtacaaacgc tgaaagcgaa gggcgatgcg ctggtccgtg aagggatcac
     7561 cattgcagac gcggtgaaac gcgtactgca tctgccgacc gtggcggaga aaactttcct
     7621 ggtgaccatc ggtgaccgca gcgtaaccgg catggtggcg cgcgatcaga tggtggggcc
     7681 gtggcaggtg ccggtcgcta actgcgcggt cactaccgcc agcctcgaca gctactacgg
     7741 tgaagcgatg gcgattggcg agcgtgcgcc ggttgcgctg ctggatttcg ccgcctctgc
     7801 ccgtctggcg gtcggtgaag cgttaaccaa tattgccgca acacagattg gcgatatcaa
     7861 acgcattaaa ctttccgcca actggatggc ggcggcaggc caccctggtg aagatgcggg
     7921 cctgtatgaa gccgttaaag ccgtgggtga agaactttgt ccggcgctgg gcctgacgat
     7981 cccggtgggt aaagactcta tgtcgatgaa aacccgctgg caggaaggta acgaagagcg
     8041 cgaaatgacg tcgccgctgt cgctggtgat ttctgcattt gcccgcgtgg aagatgtacg
     8101 tcacaccatc acgccgcagc tttgtaccga agataacgca ctgctgctga ttgatttggg
     8161 caaaggcaat aacgcgctgg gcgcaacggc gctggcgcag gtttatcgtc agcttggcga
     8221 caaaccggca gatgtacgcg atgttgtcca actgaaaggc ttctatgacg cgattcaggc
     8281 gctggttgca cagcgtaagc tgctggcgta tcacgaccgc tctgatggag gcctgctggt
     8341 aacgctggcg gaaatggcct ttgcaggtca ttgtggcatt gacgcggata tcgcctctct
     8401 gggtgacgat cgcctggcgg cgttgtttaa cgaagaactg ggtgcggtga ttcaggttcg
     8461 tgccgctgac cgtgaagcgg tcgagtccgt actggcacag catgggcttg cggattgcgt
     8521 ccattatgtt gggcaggcgg tttccggtga ccgttttgtg attaccgcca acgggcagac
     8581 tgtattcagc gaaagccgca ctacgttgcg tgtctggtgg gcagaaacta cctggcagat
     8641 gcagcgcctg cgtgacaacc cggagtgtgc cgatcaggag catcaggcga aatctaacga
     8701 cgccgatccg ggcctgaatg taaaactgtc gttcgatatc aacgaagatg tggcagcacc
     8761 gtatattgcc actggcgcac gtccgaaagt tgccgtactg cgtgagcagg gcgtgaactc
     8821 gcatgttgaa atggcggcag ctttccaacg tgggggcttt gatgctatcg acgtgcatat
     8881 gagcgacctg ctgactggac gtacgggact ggaggatttt cacgccctgg tcgcgtgcgg
     8941 aggtttctcc tacggtgatg tgctgggtgc cggtgaaggt tgggcgaagt ctatcctgtt
     9001 caatgaccgt gtacgcgatg agtttgcaac cttcttccat cgtccgcaaa cgctggccct
     9061 gggggtatgt aacggttgcc agatgatgtc taacctgcgt gaactgatcc cggggagtga
     9121 gttgtggcca cgttttgtgc gtaacacctc cgatcgcttt gaagcgcgtt tcagcctggt
     9181 tgaagtaacc caaagcccgt ctctgctgtt gcaggggatg gtgggctcgc aaatgccgat
     9241 tgctgtctct catggtgaag ggcgcgtgga agtgcgtgat gcggcgcatc tggcggcact
     9301 ggaaagcaaa gggctggtgg cactgcgcta tgtcgataac ttcggcaaag tcactgaaac
     9361 ctacccggct aacccgaacg gttcaccgaa cggtattacg gcggttacga ctgaaagtgg
     9421 tcgagtcacc attatgatgc cgcacccgga acgtgttttc cgtactgtca gcaactcctg
     9481 gcatccggaa aactggggcg aggatggccc atggatgcgc attttccgca atgcgcgtaa
     9541 gcagttgggg taagtcatca gttcattggt tttgcagccc ggatgcggca tttacgccgc
     9601 atccggcatt aagcaccaac cactatcata agctcccaac tccgggagct tttttgtgtc
     9661 tgtaaatcac gacaatgggt ggtttaccgt gtcgctttct ggcgacactt aaatgattga
     9721 ttttattgta atctaataag tttatcttaa gttgtctctt tttagcgaca cagtggctga
     9781 tttacattca aatagcgaag aagccataaa tccctcaaag caacgtataa tcagtacgtt
     9841 accaaactat tttctttatt ggcacagtta ctgcataata gtaagcagtg gctcattcac
     9901 cgacttatgt cagccccttc gggacgtgct acataaaatt cgaatgacgc acaacaaggt
     9961 gcctgccgtc caacttctga tatcagcgta gctatatcaa ccatcgggcg aaacgtcgag
    10021 ttaggcaccg ccttattcca taacaaagcc gggtaattcc cggctttgtt gtatctgaac
    10081 ttcccctcgg ttagcatcag gctattcgcg tctgacgaga gtaacacctt gaaacgctgg
    10141 cccgtttttc cccgctcatt acgacaactg gtaatgctgg catttttgct aattctacta
    10201 cccctgttgg tgctggcatg gcaagcctgg caaagcctga atgcgcttag cgatcaggcg
    10261 gcgctggtta accgcactac gcttattgat gcccggcgta gtgaagcgat gaccaacgcg
    10321 gcgctggaga tggagcgtag ctaccgtcag tattgcgtgc tggacgaccc tacgcttgcg
    10381 aaggtttatc aaagccagcg caagcgttac agcgaaatgc tcgatgccca cgcaggcgta
    10441 ctgccagacg ataaacttta ccaggcattg cgtcaggatt tgaacaatct ggcccaactt
    10501 cagtgtaaca acagtggtcc cgatgctgcc gccgccgcgc gtcttgaagc ctttgccagt
    10561 gccaataccg aaatggtgca ggccacgcgc acagtggtgt tctctcgtgg gcagcaactt
    10621 cagcgtgaaa tcgccgaacg tgggcaatat tttggttggc aatcgctggt gctatttctg
    10681 gtgagtctgg tgatggtgct acttttcacg cggatgatta tcgggccggt gaaaaatatc
    10741 gagcgcatga tcaaccgtct gggggaaggg cgttctctgg gcaatagcgt ttcgttctgt
    10801 ggaccgagcg agttacgctc ggttgggcaa cgtattcttt ggttaagtga gcgcctgtca
    10861 tggctggaat cccaacgcca tcaattttta agacatttat ctcatgaatt aaaaacgcca
    10921 ctggcgagta tgcgcgaggg cactgaatta ctggctgatc aggttgtcgg gccgcttacg
    10981 ccagagcaaa aagaggtggt gagcattctt gatagcagca gctgcaattt gcaaaaactg
    11041 atcgaacaac tgcttgatta caaccgtaaa caggcggaca gtgcggtgga actggagaat
    11101 gttgaattag caccgctggt ggagacagtg gtttctgctc atagcctgcc cgcacgggct
    11161 aaaatgatgc ataccgacgt cgatctcaaa gcaacagctt gcctggcgga gccaatgctg
    11221 ctgatgagcg tactggataa tctttactcc aatgcggtgc actacggggc tgaatccggt
    11281 aacatttgcc ttcgcagcag tttacatggt gcgcgggttt atattgatgt catcaataca
    11341 ggcacgccca ttccgcaaga ggaacgcgcc atgatcttcg accccttttt tcagggaagc
    11401 caccagcgaa aaggggcggt gaagggcagc ggtctgggat taagcattgc cagggattgt
    11461 attcgccgta tgcaagggga actgtatctg gtcgacgaga gcgggcaaga cgtttgtttc
    11521 cgcattgaat taccgtcgtc gaaaaacacg aaataatgct aacagcctat cctatcaggc
    11581 tattttattt gtcagttcgg ccccgggtag tgctcgaaat cttcacgtac gatatgtacg
    11641 ccccggtttc tccgcgctgt ccatgtccag actgtctgca acaattacgc ccactgggat
    11701 aggctttaag tctggtgaat atgcgacaca tttttcaacg attattgccc cgacggctgt
    11761 ggctggcagg gctgccatgt ctggcattgc tgggttgcgt gcagaatcac aataagccag
    11821 ccattgatac gccagcagaa gaaaaaattc cggtttatca actggctgac tatctttcta
    11881 ctgaatgtag cgacatctgg gcgctgcaag gtaaatcaac cgaaaccaat ccgctttact
    11941 ggctgcgggc gatggattgt gctgatcgtt taatgcctgc gcagtcgcgt cagcaagctc
    12001 gccaatatga cgacggcagc tggcaaaata cctttaaaca agggattttg ctcgccgacg
    12061 ccaaaattac gccgtatgag cgccgccagc tggtcgcgcg tattgaagcg ttaagtaccg
    12121 agatcccggc acaggttcgc ccactgtatc aactctggcg cgacggtcag gcgctgcaac
    12181 tgcaactggc ggaagagcgc caacgttaca gtaaactcca gcagtccagc gacagcgagc
    12241 tggatacatt gcgccagcaa catcacgttc tacagcagca actggaactc accacccgca
    12301 agctggaaaa cctgaccgat attgaacgcc agctctcgac ccgcaaaccg gctggaaatt
    12361 tctcgccgga tacgccgcac gagagtgaaa agcccgcgcc atccacccat gaggtcactc
    12421 ctgatgagcc ataaacctgc gcatttatta ttggtcgatg acgatctggg attgctgaaa
    12481 ctgcttggcc tgcgcctgac cagcgaaggc tacagtgtgg tcacggcgga aagtggcgct
    12541 gaaggattac gggtactgaa tcgcgaaaaa gtagatttag ttatcagcga cctgcggatg
    12601 gatgaaatgg acggtatgca gctatttgct gaaatccaga aagtgcagcc gggaatgcca
    12661 gtaattattc ttactgcgca tggttctatc cccgatgccg ttgctgcaac acagcagggc
    12721 gtttttagtt tcctcaccaa gcctgtcgat aaagatgcgc tatatcaggc aattgacgat
    12781 gcgctggagc aatccgcgcc agccaccgat gaacgctggc gcgaggcaat tgtcacccgc
    12841 agcccgctga tgctgcgttt gctggaacag gcgcggctgg tggcgcaatc agacgtcagc
    12901 gttttgatta acggtcagag cggcaccggg aaagagattt tcgcccaggc tatccacaac
    12961 gccagcccgc gcaacagcaa accatttatt gctattaact gtggcgcatt gcccgagcaa
    13021 ttgctggagt cggagctgtt tggtcatgcg cgtggcgcgt ttactggcgc tgtcagcaat
    13081 cgcgaaggtt tattccaggc ggcggaaggc ggaacgctat ttctcgatga aattggcgat
    13141 atgcctgcgc cgttacaggt caaactgctg cgcgtgttgc aggagcgtaa agtgcgcccg
    13201 ctgggcagta accgcgatat tgatatcaat gtgcggatta tttctgccac tcaccgtgat
    13261 ctgtcaaaag cgatggcgcg cggggaattc cgcgaagatc tctattaccg cctcaacgtt
    13321 gtcagcctga aaattccggc actggcggag cgcacagaag acattccgct actggcaaat
    13381 cacctgttgc gccaggcggc agagcgacat aaaccgtttg tccgcgcgtt ctctaccgat
    13441 gcgatgaaac gcctgatgac cgcgagctgg ccgggtaatg tgcgccagtt ggtcaacgtg
    13501 attgaacagt gcgtggcgct gacctcatct ccggtgatta gtgatgcgct ggtggagcag
    13561 gcgctggagg gtgaaaatac ggcgctgcca acctttgttg aggcacgtaa tcattttgaa
    13621 ctcaactatt tgcgtaagct gctgcaaatc accaaaggca acgtcaccca cgcggcgaga
    13681 atggcggggc gcaaccggac agaattttat aaactgcttt cccgacacga gctggatgca
    13741 aacgatttca aggaatgaat tggcgttatg tgttacgttt agcagatcaa aagacaggca
    13801 accttttcaa ggaatagcat gaaaaagatt gatgcgatta taaaaccctt caagctggac
    13861 gatgtccgcg aagcactggc cgaagtcggt attaccggca tgacggtgac cgaagtgaaa
    13921 ggctttggtc gccagaaagg ccataccgag ctgtaccgcg gcgcggagta tatggtggat
    13981 tttctgccga aagtgaaaat tgagattgtc gtaccggacg acattgtcga tacctgtgtc
    14041 gataccatta ttcgcacggc gcaaaccggc aaaatcggtg acggtaaaat cttcgtcttt
    14101 gacgtggctc gggtcattcg catccgtacc ggtgaagagg acgacgcggc aatttaaacc
    14161 gcgtcaaggg ttgccggatg tttccatccg gcaacatcaa attacagcac cttatgcggg
    14221 ccaaagcatt cgtaatgaat gttttcctgc ttcacgccca gatccactaa ctgtttcgcg
    14281 gtaaactgca tgaagccaac cgggccgcag agatagaact gcattgtcgg atcgctgaac
    14341 gcaccttcca gtttgctcaa atccatcaga ccttcgctat caaactgacc tttagcgcga
    14401 tcggcttcgc tcggctgacg ataccaggtg tgcgcggtaa agcgcggcag tgactgcccc
    14461 agttccttaa cttcatcagc aaaggcgtga acttcgccat tttctgccgc atggaaccag
    14521 ttcacttgtg ctgtgtggcc tgcttttgcc agcgtgtcga gcattgccag cattggcgtt
    14581 tgaccaacac cggtagagat taacgtcact ggtgtgtcat ctgcgacagc cataaagaaa
    14641 tcacctgccg gagcgaccag tttcacgaca tcgccaacat tggcgtgatt gtgcaaccag
    14701 ttggatacca gcccaccctc ttcgcgtttc accgcgatac gatagccttt gccatccggt
    14761 ttgcgagtca aagagtactg acgaatttcc tgatgcggga aaccttccgg cttcagccag
    14821 acgccgagat attgccccgg acggtattct gccactgcgc caccgtcgac cggctccagt
    14881 tcgaagctgg tgataagcgc gctgcgcggt gttttagcca caatgcggaa atcgcgagta
    14941 ccttcccaac caccggcttt gctggcgttt tcgttataga tttccgcctc gcgattgata
    15001 aatacattag ccagtacacc ataggcttta ccccacgcgt ccagcacttc ctgccccggg
    15061 ctgaacattt cgtccagcgt tgccaacagg tgttcaccga cgatgttgta ctgttccggt
    15121 ttgatctgga agctggtgtg cttctgcgcg attttttcta ccgctggcag cagcgcaggc
    15181 aggttttcaa tattactggc gtaggcggca atagcgttaa acagggcttc acgttgatcg
    15241 ccattacgct ggttactcat gttaaaaatt tctttgagtt ctgggttatg agtaaacata
    15301 cggtcgtaga aatgggcggt taactttggc cccgtttcca ccagtaaagg gatggtggct
    15361 tttactgtag cgatggtttg agcgtcaagc atatggtctt ccttttttgc atcttaattg
    15421 atgtatctca aatgcatctt ataaaaaata gccctgcaat gtaaatggtt ctttggtgtt
    15481 tttcagaaag aatgtgatga agtgaaaaat ttgcatcaca aacctgaaaa gaaatccgtt
    15541 tccggttgca agctctttat tctccaaagc cttgcgtagc ctgaaggtaa tcgtttgcgt
    15601 aaattccttt gtcaagacct gttatcgcac aatgattcgg ttatactgtt cgccgttgtc
    15661 caacaggacc gcctataaag gccaaaaatt ttattgttag ctgagtcagg agatgcggat
    15721 gttaaagcgt gaaatgaaca ttgccgatta tgatgccgaa ctgtggcagg ctatggagca
    15781 ggaaaaagta cgtcaggaag agcacatcga actgatcgcc tccgaaaact acaccagccc
    15841 gcgcgtaatg caggcgcagg gttctcagct gaccaacaaa tatgctgaag gttatccggg
    15901 caaacgctac tacggcggtt gcgagtatgt tgatatcgtt gaacaactgg cgatcgatcg
    15961 tgcgaaagaa ctgttcggcg ctgactacgc taacgtccag ccgcactccg gctcccaggc
    16021 taactttgcg gtctacaccg cgctgctgga accaggtgat accgttctgg gtatgaacct
    16081 ggcgcatggc ggtcacctga ctcacggttc tccggttaac ttctccggta aactgtacaa
    16141 catcgttcct tacggtatcg atgctaccgg tcatatcgat tacgccgatc tggaaaaaca
    16201 agccaaagaa cacaagccga aaatgattat cggtggtttc tctgcatatt ccggcgtggt
    16261 tgactgggcg aaaatgcgtg aaatcgctga cagcatcggt gcttatctgt tcgttgatat
    16321 ggcgcacgtt gcgggcctgg ttgctgctgg cgtctacccg aacccggttc ctcatgctca
    16381 cgttgttact accaccactc acaaaaccct ggcgggtccg cgcggcggcc tgatcttggc
    16441 gaaaggtggt agcgaagagc tgtacaaaaa actgaactct gccgttttcc ctggtggtca
    16501 gggcggtccg ttgatgcacg taatcgccgg taaagcggtt gctctgaaag aagcgatgga
    16561 gcctgagttc aaaacttacc agcagcaggt cgcgaaaaac gctaaagcga tggtagaagt
    16621 gttcctcgag cgcggctaca aagtggtttc cggcggcact gataaccacc tgttcctggt
    16681 tgatctggtt gataaaaacc tgaccggtaa agaagcagac gccgctctgg gccgtgctaa
    16741 catcaccgtc aacaaaaaca gcgtaccgaa cgatccgaag agcccgtttg tgacctccgg
    16801 tattcgtgta ggtactccgg cgattacccg tcgcggcttt aaagaagccg aagcgaaaga
    16861 actggctggc tggatgtgtg acgtgctgga cagcatcaat gatgaagccg ttatcgagcg
    16921 catcaaaggt aaagttctcg acatctgcgc acgttacccg gtttacgcat aagcgaaacg
    16981 gtgatttgct gacaatgtgc tcgttgttca tgttggatgc ggcatgaaca ccttctcagg
    17041 cctacaaaat cttgcaaatt caatatattg caatctccgt gtaggcctga taagcgtagc
    17101 gcatcaggca atttttcgtt tatgatcatc aaggcttcct tcgggaagcc tttctacgtt
    17161 atcgcgccat caaatctgtc gtaactgcgc ctcaacatac aaatagccaa ttcccagcac
    17221 ctgttgtgcg cggcttaatt gcccgaagcc aatttgcgtc gctttcacgc tcggtccttc
    17281 gtcgcggtcg aacgggttaa atcctgtctg gcgaataata gtattaagcc agttctcacc
    17341 aaaggcacaa ctgcgaccgt acaaccaaat ctgattaata ttgaggatgt tgaggaagtt
    17401 atacagactc aaaccaatgg cattggcaga gcgatcaacc cagctggtga tccacggttc
    17461 tccactctgc caggcagcga ttaactgcgc ggttgtcagt ttttcaggct caagctgagt
    17521 attaaccggt tgtgatttta gccatacccg cgcctgtttt tttaatgcac ttaacgaggc
    17581 gacggtttcc aggctgccgt aacgtccaca gtcgcagacg acgccatccg gattaacgat
    17641 ggtgtgacca atctgtccgc taccatacaa actgccgcga taaatttgct cgttaatcac
    17701 gaacgacgag ccaatgccgt aatcaacgtt gatcacgcag aaatcccgtt cctgcgaatt
    17761 attttgccat ttctccgcca gcgccagcat cacgcagtca ttatcgacca gcacccgaat
    17821 gcccagcttc tcttccagca gatacttcac ctcaactggc gttgtccacg gcgcttgcgg
    17881 catggtttgc gatacgccgg tcaccggatc aacctgcccg tggattgcca gcgccagatt
    17941 gatggtgcgg tccggccaca atttacggtg gcgatgccag catttttcga tttcggacag
    18001 cagcgcctgc ggcgtcggta catcaatctg taaatactca aattcgcctt tcggacttaa
    18061 acaagcatta gcaacctgac actcaatact ggtgggcgtc acgttcaggc acagcgtcca
    18121 gtcaccttcc ggcgcaatca gccatgtacc gctactatgc ccgcgcgtct ggctttcatc
    18181 gtcaatattc accacccgtt tttcgctttc cagttcctgc aaaatattac tgactgccgg
    18241 aatcgaaatt tgcgccagcc gggccagcgt tgatttattg gcgcgctttt gccggtacag
    18301 cagttccaga atcacgcatt tgttatggtg gcgaatctgt tgattgttaa tgcaggctct
    18361 catcttcact aaaccttgtt tagttaattg cgtgattatt ccgcgaaaac attgccagat
    18421 tgttcaatac actgccacaa atcttttaat tcagtatgtc ttgttaatat tgagggcacc
    18481 atgactccag taaaagtgtg gcaagagcgc gttgagatcc cgacctatga aaccgggccg
    18541 caggatatac atcccatgtt cctggaaaat cgcgtttatc agggatcgtc cggcgcggtt
    18601 tatccctacg gcgtgaccga tacgctgagc gagcagaaaa ccctgaaatc ctagcaggcg
    18661 gtgtggctgg aaaacgacta catcaaagtg atgatcctgc cggaactggg cggtcgggtg
    18721 catcgcgcat gggataaagt gaaacagcgc gattttgtct atcacaatga agtcattaaa
    18781 cctgcgctgg tggggatgct gggaccgtgg atctccggcg ggattgagtt taactggccg
    18841 caacaccatc gcccgaccac ctttatgccc gttgatttca ccctcgaagc ccatgaagac
    18901 ggtgcacaga cggtgtgggt cggcgaaacg gagccgatgc atggtttaca ggtgatgaca
    18961 ggtttcaccc tgcgccctga ccgggcggca ctggaaatcg ccagccgcgt ctataacggc
    19021 aacgccacgc cgcgtcattt cttgtggtgg gccaacccgg cagtgaaagg gggtgaaggg
    19081 catcagagcg ttttcccacc ggatgtgact gcggtgtttg atcacggcaa acgggccgtc
    19141 tccgctttcc ccatcgccac cggcacttac tacaaagtgg actactccgc cggagtggac
    19201 atttctcgct ataaaaatgt gcctgttcca acctcatata tggccgaaaa atcgcagtac
    19261 gattttgtcg gcgcgtggtg tcacgatgaa gatggcggtt tgctgcacgt tgccaaccac
    19321 catattgcgc caggtaaaaa acagtggagt tggggacaca gtgaatttgg ccaggcgtgg
    19381 gataagagtc tgaccgacaa taacggcccg tatatcgaac tgatgaccgg tatttttgcc
    19441 gataaccagc ctgattttac ctggcttgat gcttacgaag agaagcgttt cgagcagtat
    19501 ttcctgcctt atcattctct gggcatggtg caaaatgcct cccgcgatgc ggtgataaaa
    19561 ctccagcgta gtgagcgggg gattgagtgg gggctgtatg ccatctctcc gttgaacgga
    19621 taccgtctgg cgatccgcga aatcggcaaa tgcaacgcgt tgctcgatga tgccgtggcc
    19681 ctgacaccag cgaccgccat ccagggcgtg ttgcacggta tcaatcctga cagactgacc
    19741 attgaactct ccgatgccga cggcaatatt gtactgagtt atcaggaaca tcagccgcag
    19801 gagttgccgt tgccggacgt cgccaaagcg ccactggcag cacaagacat taccagtaca
    19861 gatgaagcct ggtttatcgg tcagcatctg gagcaatatc atcacgccag ccgttcaccg
    19921 ttcgattact acctgcgcgg cgtggcgctg gacccgctgg attatcgctg taacctggcg
    19981 ctggcgatgc tggaatataa ccgcgcagat ttcccgcaag cggtggcgta tgccagtcag
    20041 gcgctgaaac gcgcacatgc gctgaacaaa aacccgcagt gtggacaagc aagtttgatt
    20101 cgcgccagtg cttacgaacg tcagggacaa tatcaacaag ccgaagagga tttctggcgt
    20161 gcggtctgga gcggcaatag taaagcaggc ggctattatg gtctggcacg actggcggcg
    20221 cgtaatgata acttcgacgc gggtctggat ttttgccaac aaagtcttcg tgcctgccca
    20281 accaatcagg aagtgctttg cttgcataat ctgctgctgg tgttaagtgg tcgtcaggac
    20341 aacgcgcgtt tgcagcgcga gaaactgctg cgtgattatc cgctgaacgc cactctgtgg
    20401 tggctgaact ggttcgatgg tcgtagcgaa tcagccctcg cgcagtggcg cggtctgtgt
    20461 cagggacgcg acgttaacgc tctgatgacc gccgggcaac tgattaactg gggaatgccc
    20521 accctggcgg cagagatgct gaacgcactg gactgccagc gcacgctgcc gctttacctg
    20581 caagccagct tgctgccgaa agccgaacgt ggcgaactgg tcgcaaaagc cattgatgtc
    20641 ttcccgcagt ttgtccgttt cccgaatacg ctggaagaag tggcggcgct ggagagtatt
    20701 gaagagtgct ggtttgctcg ccatttactg gcctgcttct actacaacaa acgtagctac
    20761 aacaaagcca ttgccttatg gcaacgttgc gtagagatgt cgccggagtt tgccgacggc
    20821 tggcgcgggt tagcgatcca tgcgtggaat aagaaacacg attatgagct ggccgcgcgt
    20881 tatcttgata atgcttatca gcttgcgccg caggatgcac gtctgctttt cgaacgggat
    20941 ttgctggata agttaagtgg agccacaccg gagaaacgac tggcacgtct ggaaaataat
    21001 ctggaaattg cgctgaaatg cgacgacatg accgcagaac tgctcaattt gtggcatctc
    21061 acgggtcagg cagacaaagc ggcggacatt ctcgccacgc gtaaattcca cccgtgggaa
    21121 ggcggggaag ggaaggtcac cagtcagttt atcctcaacc agttattacg cgcctggcag
    21181 catcttgatg ccagagagcc gcagcaggcc agcgaactgc ttcatgccgc gctgtattat
    21241 ccggagaatt taagcgaagg ccgtttaccg gggcaaactg ataacgacat ctggttctgg
    21301 caggcgatat gcgccaacgc gcagggcgat gaaactgaag cgatgcgttg tttacgtctg
    21361 gcggcgaccg gcgatcgcac cattaacatc cacagttatt acaacgatca gccggttgat
    21421 tatctcttct ggcaaggaat ggcgctgcga ctgctgggtg aacagcaaac cgcacagcaa
    21481 ctgtttagtg aaatgaaaca gtgggcgcaa gagatggcga aaaccagtat tgaggcggat
    21541 ttctttgctg tttcacaacc tgacctgtta tcgctgtatg gcgatttaca acagcagcat
    21601 aaagaaaaat gcctgatggt ggcgatgctg gcgtccgcgg gactcgggga ggttgcgcaa
    21661 tatgagtctg ctcgcgctga attgacggcg attaatccgg cctggccgaa agcggcatta
    21721 ttcaccactg tgatgccttt tatttttaac tacgttcact aaaccctatt aactgtaata
    21781 tttgagcggc acgcgctgcc gctcatcaca aaataattag aataagccgt tgttaataaa
    21841 aaccctacag gaggcactat gcctaaaaaa atgagaacaa cccgtaatct attattaatg
    21901 gctacgctat taggtagcgc actatttgcc agggctgcgg ataaagaaat gaccattggt
    21961 gcaatatacc ttgataccca gggatattac gctggagtgc gtcagggcgt tcaggatgcg
    22021 gcaaaagatt cttcagtaca ggtacagtta attgaaacta acgcccaggg ggatatttcg
    22081 aaagaaagta cctttgttga taccctcgtg gcgcgtaatg tcgatgccat tattttatcg
    22141 gcagtgtctg aaaatggcag tagccgtacc gttcgtcgcg ccagtgaagc gggtattccg
    22201 gtgatttgct acaacacctg tattaatcaa aagggtgtcg ataaatatgt ctcggcgtat
    22261 ctggtcggcg atccactgga atttggtaaa aaactgggta acgctgccgc cgattatttt
    22321 attgccaata aaattgacca gccgaaaatt gccgtcatca attgcgaagc ctttgaagtt
    22381 tgtgtgcagc gacgtaaagg atttgaagaa gtattaaaat cccgcgttcc cggcgcgcaa
    22441 attgtcgcta accaggaagg gactgtttta gataaagcga tttccgttgg tgaaaaactg
    22501 attatctcca cgccggatct caacgccatt atgggggagt cgggcggtgc gacactcggc
    22561 gcggtaaaag cggtacgtaa tcaaaatcag gccggaaaaa ttgctgtttt cggttcggat
    22621 atgacaaccg aaattgctca ggagctggaa aacaatcagg tgctgaaagc ggtagtggat
    22681 atttccggta agaaaatggg caatgctgtt ttcgcgcaaa cattgaaggt tatcaataaa
    22741 caagccgacg gtgaaaaagt gattcaggtg ccaatcgatc tctataccaa aacggaagat
    22801 ggtaaacagt ggctggcaac gcacgttgat ggtctgccct aatcgctgat tcgcagaggt
    22861 ccctatgttc acggcaacag aggcagtccc ggtagcaaaa gtggtggcag gaaataagcg
    22921 ttaccccggc gtcgttgcgc tggataacgt taacttcacg ctcaataaag gcgaagttcg
    22981 tgcgctgtta ggtaaaaacg gcgcgggcaa atcgactctc attcgaatgc ttaccggcag
    23041 cgaacgcccg gatagcggtg atatctggat tggcgagacg cgactggaag gtgacgaagc
    23101 tacgttgact cgccgtgccg ctgaactggg ggttcgtgcg gtttatcagg aattaagtct
    23161 ggtggaaggg ctgacggtgg cagaaaacct ctgccttggt caatggcctc gccgcaacgg
    23221 catgattgat tacctgcaaa tggcgcagga tgcccaacgt tgcttacagg cgctgggcgt
    23281 tgacgttagt cctgaacaac ttgtttcaac gctaagcccg gcgcaaaagc aactggtgga
    23341 aattgcacgg gtgatgaagg gcgagccgcg cgtggtcatt cttgatgaac ccaccagctc
    23401 gcttgccagt gcagaagttg aactggtgat cagtgcggtg aaaaagatgt cggcactggg
    23461 cgtggcggtg atttatgtca gccaccggat ggaagaaatc cgccgcattg cctcctatgc
    23521 caccgttatg cgcgatggtc aggtggcggg cgatgtgatg ctcgaaaaca cctccacgca
    23581 tcatattgtg tcgctaatgc tagggcgtga tcacgttgat attgcgccgg ttgcccctca
    23641 ggaaattgtg gatcaggccg tactggaagt ccgtgcgtta cgccataagc ccaagctgga
    23701 ggatatcagc tttacgctac gtcgtggcga agtgcttggc attgctggcc tgctgggggc
    23761 agggcgcagt gaattgctga aggcgattgt tgggctggag acgtatgaac agggcgaaat
    23821 tgttatcaac ggcgagaaaa tcatgcgccc cgattacggc gacatgctga aacgcggcat
    23881 tggctatacg ccagaaaacc gcaaagaagc ggggatcatt ccctggctag gcgttgacga
    23941 aaatacagtg ctgaccaatc ggcaaaaaat cagcaccaac ggtgtgctgc aatggtccac
    24001 catccgccgc ctgaccgaag aggtgatgca gcggatgacg gtcaaggctg ccagtagcga
    24061 aacacccatc ggcacgcttt ctggtggcaa tcagcaaaaa gtggtgatcg gtcgttgggt
    24121 ctatgccgcc agccagattt tgttgctcga cgagccaacg cgcggcgtcg atatcgaagc
    24181 caaacagcag atttaccgta ttgtccgtga gctggctgcc gaaggaaaaa gcgtggtgtt
    24241 tatctccagt gaagtggagg aattgccgct ggtgtgtgac cgcatcctgt tattacagca
    24301 cggcacgttc tcgcaggagt ttcactcgcc ggtcaatgtg gatgagctga tgtccgccat
    24361 tctgtctgtg cactgatgaa ttttaccgag gaacctgaag atgtatgctt cgtcattacc
    24421 attgccgcag ggcaagagcg tctcgctcaa acaatttgtc agtcgccata ttaatgagat
    24481 cggtttgctg gtggtgattg ccattcttta tctggtcttc tccctgaacg cgcctggctt
    24541 tatctcattg aataaccaga tgaacgtgct gcgcgatgcc gccaccattg ggattgccgc
    24601 ctgggcgatg acgctgatta ttatctccgg tgaaattgat gtcagcgttg ggccgatggt
    24661 ggcttttgtc tcggtgtgcc tggcattttt gctgcaattt gaagttccgt tggcgattgc
    24721 ttgtctgctg gtgttgctgt taggcgcgct gatgggaacg ctcgccgggg tgctgcgcgg
    24781 cgtgtttaac gtaccaagtt tcgttgccac actggggctg tggagcgctc tgcgcggaat
    24841 ggggctgttt atgaccaacg cactgccagt gccgattgac gaaaacgagg tgctggactg
    24901 gctgggcgga caatttctcg gtgtgccagt atccgcgctg atcatgatgg tgttgtttgc
    24961 gctgtttgtg ttcattagcc gaaaaaccgc cttcgggcgc tcggtttttg ccgttggcgg
    25021 taatgccacg gcggcgcagt tgtgcggtat taacgttcgt cgagtgcgca ttcttatctt
    25081 taccctttcg ggattattag cggcggtgac cggcattttg ttggcggcac gcctcggttc
    25141 cggtaacgca ggtgccgcaa acggtctgga gtttgacgtc atcgccgcgg tcgtggtcgg
    25201 cggtacggca ctttccggcg gtcgcggttc cctgttcggt acattgcttg gtgtgctggt
    25261 gattacgcta atcggtaacg gtctggtgct gctcggtatt aactcctttt tccagcaggt
    25321 ggtacgcggc gtcatcatcg tggtggcggt gctggcgaat atcttgctga cccagcgaag
    25381 cagtaaagcg aaacgctaaa ccttaacgcc cctggcgcgg gtgcggcagg gatgaaaatt
    25441 aatcgtattg ttaatgaggg atttatgaaa acgatgctgg ctgcttattt actaggaaat
    25501 tcaaccgtcg atctgcggga agttgcggtg ccgacgccgg ggattaacca ggtactgatc
    25561 aaaatgaaat cctccgggat ttgcggaagc gatgtccact atatctatca tcaacaccgt
    25621 gccacggcgg cggcacccga taaaccgtta taccagggat ttatcaacgg tcatgaaccg
    25681 tgcgggcaga ttgtggcgat ggggcaaggc tgccgccatt ttaaagaggg cgaccgcgtg
    25741 ctggtgtatc acatttctgg ctgtggtttt tgcccgaact gccgccgcgg ctttcctatc
    25801 tcttgtactg gcaaaggaaa agcggcttac ggctggcagc gtgacggcgg tcatgccgaa
    25861 tatctgctgg cggaagaaaa agatctgatc ctcctgccgg atgcgctgag ctacgaagat
    25921 ggtgcgttta tcagttgcgg cgttggcacg gcctatgaag ggattttgcg cggcgaagtt
    25981 tccggcagcg ataacgtgct ggtggtcggt ctggggccgg tcggcatgat ggcgatgatg
    26041 ctggcgaaag gtcgcggtgc aaaacggatc atcggcgttg atatgctgcc ggaacgtctg
    26101 gcgatggcaa aacagttagg ggggatggat cacggctatt tagcaaccac cgaaggtctg
    26161 ccgcagataa ttgctgaact tacccacggt ggcgcggatg ttgcgctcga ttgttccggt
    26221 aatgccgcag gtcgcttgct ggcactgcaa tccactgctg actgggggcg ggtggtttac
    26281 attggtgaaa ccggaaaagt ggaattcgag gtcagcgctg atctgatgca ccatcaacgg
    26341 cggattatcg gctcctgggt gaccagtctg ttccatatgg aaaaatgcgc ccacgatttg
    26401 actgactgga agctgtggcc gcgtaacgcc attacccatc gcttctcgct agaacaggca
    26461 ggtgatgcct atgtgctgat ggcgagcggc aaatgcggga aagttgtgat taacttcccg
    26521 gattaaggag ttagcggatg accatctata ccttatccca cgggtcgcta aagctggacg
    26581 tttctgacca gggcggcgta atcgaaggct tctggcgcga tacgacgccg ctcttacgtc
    26641 ctggtaaaaa aagcggtgtg gcaaccgatg cctcatgttt tccgctggtg ccatttgcaa
    26701 accgggtgag cggcaaccgt tttgtctggc aggggcgtga gtatcaactg caaccgaatg
    26761 ttgagtggga tgcgcactat ctacacggcg atggctggct gggcgagtgg caatgtgtct
    26821 cgcacagcga tgatagtttg tgtctggtgt atgaacatcg cagcggtgtc tatcactatc
    26881 gggtaagtca ggcgtttcat ttaacggcgg atacgctgac ggtgacgctc tctgtcacca
    26941 atcaaggggc agagacgctg ccatttggta ccggctggca tccttatttt ccgttgtcac
    27001 cacaaacgcg gattcaggcg caggcgagcg gttactggct ggaacgggag cagtggctgg
    27061 cgggtgagtt ttgcgagcag ctaccgcagg aactggattt taaccagctc gcgccgttgc
    27121 cgcgtcagtg ggtgaacaat ggttttgccg gatggaatgg tcaggcccgc atcgagcagc
    27181 cgcaggaagg ttatgccatc atcatggaaa cgacgccacc tgcaccgtgt tatttcatct
    27241 ttgtttccga ccctgcgttt gataaaggat atgcgtttga tttcttctgt ctggaaccga
    27301 tgagccatgc gccggatgat catcatcgcc cggaaggggg tgatctcatt gcgctagcac
    27361 caggggaatc aacaacctca gagatgtcgt tgcgggtaga gtggctgtaa tttataaaat
    27421 gccggataag gcgttcacgc cgcatccggc atctttccta acgccgatcg agagaaattg
    27481 cccccggtcc ggtaattgcc aatagcaaga acgcgccagc gatactgaca ttcttccaga
    27541 aattaatcat atttggccca accgcatcgc cggtcatatc ccagtaatgg tgaccaatca
    27601 ccgccgtacc cagcgtgtag aaaataaaca gcaccgccag cggacgggtg aaaaagccaa
    27661 gcacgattaa tatcgcggcg ggcacttcca taactaccgc aataatcgct gccagcatcg
    27721 gcattggcgc gcccaacgag gccatatatt ggaccgtacc gtcaaagccc atcattttgg
    27781 gaaaaccaaa aataatgaaa attaagacca cggcgatacg ggcaattaat aacaaaacgg
    27841 ggcgggcagc tccaaaatca aaataacgta gtgtgttcat aaccaatccc tttgcaagat
    27901 aatgtgattt aaacgtaata cacactcttg cacctcgcca cgccccaggc attgagatat
    27961 tcgctaatta tcctgattac agtgatttaa gcgcgatgtt ctcatctatc agcagtttcg
    28021 catcaaacgt tttgccgctc tggatccatt tgcgaattgg gcgaatctca cggccctgat
    28081 tcagcgtgac cgcaccgata agcacgccgt tttgcagatt aaaccaaatc gccttctgag
    28141 tttccgggtt gccacgacaa agccagtcat cgccacgcat atcgccaata aactgtaagt
    28201 tatcactgta ctgatcgctc cagaaccacg gcggcggcag tagcggtagc ggtagcccta
    28261 acattgcggc agcggcaatt tgcgcctggt tattggcgtt ttcccagctt tcgcagcggt
    28321 gtagtgcacc attatcaaga cgagtgattg ccacatcgcc accggcaaag atcgcgggat
    28381 cgcaggtgcg gcaagcctca tcaatgacaa tgccattggc agtatcaagg ttggcctcgc
    28441 gagccagttg ctcgttggcg ctgataccaa taccgtaaat caccacatca gcctgaagcg
    28501 tttccccact ttgcagcgtc agttctactt tttcaccatc gaccacatgt tcaatggcat
    28561 tattgagcag aatgcgcaca ccagcctgct gatggcgctg taaaagatag cgttgcacgg
    28621 gcggtggtgc attacggccc atgacggttg ccgccagttc aatcactgtc accttacatc
    28681 tgcgctgcgc tgcgtggcgc tggcagccag ttccagacca atagttccgg caccgacaat
    28741 cacgactgac cgttcgggct gcagaacttc tcgcagtctg gcggcatcac cggcatggtg
    28801 tagggtaaag cagcgttctc ccagtgcatc aagcaacggc agcggtcgag ctgccgcgcc
    28861 ggttgctata aaaagctgat cccagtgcca gctttcgccg ttggttaaca ctaactctcg
    28921 tgtgtcgcga cccagcgttt tgatggttac accggaatgc agatgaacat tgttttcctg
    28981 ccaccagtta gcgggtaaca cctgctgtaa ctgcggggaa tcttccagca acatggattt
    29041 cgagagtgga ggtcgttcat aaggaagatg tcgctcatcg gaaaacagat gcagctcacc
    29101 ggtgaaccct tgctggcgta gcgaggccgc agccattgcc gccgcttgcc cgccaccgac
    29161 aataatgatc gttttttctt tcatcgtgct tccttaaaga tccagcccag ccgctacgtg
    29221 gcgaatgccg cgaatcgcta aacccgcatc agcgttgatc atcacaccgc ttaatgcgcg
    29281 attattgcgc cgcgatgcca acatcacata cggccccgta aaatccgccg gttgcgggaa
    29341 aaattgcagc ggtaaaatgg cggcaatttt ctccggcgtc agagactgca ttatcgaggt
    29401 ttcactttgc ccgagcgcct gtgggccgcg caggtcgctg gccataccac acgggccgac
    29461 gccattcacc cgcactttcg gtgccagttc ataagccagt tggcgaataa gtccggttgc
    29521 ggcatgttta ctggtggtgt acagcgggcc accgccgcca ggataccagg cggcatttga
    29581 cagtgtgaaa atcatgctgc cttcactggc gattaacgcc ggagcgcagg cttttgcgcc
    29641 cagcaggtaa ccgagtacgt tgacgttaaa cagctcgtgg aagccggttt cgagcgtctc
    29701 tgcgggagta ttaaccagtg aggcattgtg atcccagatg cctgcattgc cgataaaaca
    29761 atccagcttg ccggaacgag tcaggatctg atcgaccgcg cgttgataat cggcataaca
    29821 ggtcacgtta ccttccaccg ccagaatatg ttcgccaaat cgctgacgca gactggcgac
    29881 ttttgccgcc gacagttcca gcgtggcaac ttgcgcgcct tcttcgataa atcgctcgac
    29941 cagcgccagc cctaatcccg atccgccgcc ggtaataaaa atggactcgt tatgcagatc
    30001 gctcatggct gcgcctccgg taagtcgatg aaaatgtcac caccttcaac gtgtactgga
    30061 taagtggtga gcggatcggt ggcgggcagg cataacgctt tccccgtttt caggcaaaaa
    30121 ctggcggcgt gtagcgggca ctccaccgtg gcgtcatctt ccagataccc ttctgacatc
    30181 gacgcattac catggctgca acgatcgtta atggcataaa actcgccgcc cacgttgaac
    30241 aaggcgatga cgggcgaggt atctatccgg agagcctcac cctccggcac atccgcaacg
    30301 ggacacgcat aaattcgatt catcagaaca gtacactcag gttatgggaa gtgatcaccg
    30361 cttgatccag gacgatatcc cgttccagca agcgccagtt gtcatcttcc agacggcgaa
    30421 ctttgtcgaa acgcgttcca acatagaatg tttcatcgcg ctctttttgt gcccgataaa
    30481 gtagataatt caccctcacg gcaaatacgt ttgggatgtc ggtttcgctt acctggcagt
    30541 tgctgattaa gtgacgagtg cgtgacggcg gctcttctgc ccaggcaatg cccgtttcca
    30601 gacgggcgat tcgccgctcc agctggtctt tggtgtcatt gaaaatccag gtcgtcggcg
    30661 gctggacgcc tttgcgacgg tcgcgtgttt gcgcgttaac cgtggtgcgc atggtgtaac
    30721 gaatctcttc gtcgagctgt gccaaccagt cacgaaattt ccagtcatcc agcagactgg
    30781 cttcgtgaaa gagaaactgg ctaatgcggt gatgtaactc cagtgaaact tgcgcactca
    30841 tttcatcacc tcctgctggt aagcggcggt tttatcgagc acttcctgcc agctttcgct
    30901 actcagaaga tcggcccagc gttggtacat tccacgagcg gcagtttctg aaaagatata
    30961 gttagtaatg ccaggaatgc cgtcgtcgcg gcgcttttcc tgaccaagcc ccatttccag
    31021 acacagtttg ctgttgcggg cgcggtggcc tttaagcaat ttctggattt cacaccagtt
    31081 ctccgagtca tcctgctcga gaaaaccagc aggaccaaaa gcacgagtgg cgctgttttc
    31141 aaaagcggct ttaacttcat cggaggcggc tttgtcagta atacagaacg cccacacttc
    31201 aacttgatca gggccgcgcg gatgccagac gcggagtgtg gcagtgccgt ttagccatga
    31261 aagcgtgggg aatatattgt tatgacccgc caggcgcagg gcgcgaactt cacctaaacg
    31321 ttgttctgct tcggcatagg tttcgcggta atagcttgaa actgcgccat cgacccagac
    31381 attagcatcc ggtttttcag taaagaagaa accgctaccg tgaccgtcct gaccaaattg
    31441 cagcgcatct ttggcggttt cccacaccgg gcgggcggtt tgtccatcac cgaggcgctt
    31501 atcgctgcca tcatctttcg cccctaatac ctgaacggca gaagcatggc tgaacagagc
    31561 atgatactgg tcactggcga actgctctgc cgggaatttc cagttacagt tgatcaccca
    31621 cttttgtacg ccgccgacaa tttcggtgcc gccttcgcga cgatccagca tgccatccag
    31681 ataccaggca atgtcaccca ggtaatcatg caggcccggt gcgctggtat cccagttgcc
    31741 aaaaatcagt cctttataac tctccacaca aggaacttcg tttagccccc agtgggattt
    31801 acacaaccct tgtgggtagg cgcgaggttc cagcggtaca tcgatcaact cgccgttaat
    31861 gccataagac cagccgtgat acgggcaggt aaaggcgcga gtgttgccac aatctgcata
    31921 actcacacgc atggcccggt ggcggcattg gttgagaaac gccttgatgc tgccgtcttt
    31981 ctggcgcacc acgacaactg catcttctcc catgtaggtg ttaaagaaat caccgggttt
    32041 tgggatctgg ctttcgtggg cgagaaatag ccagcaacga ccgaaaatac gctcaagctc
    32101 cagttggtaa atgtccgggt cggtataaat acgcggagtg acccgaccgt tttgggtgtc
    32161 aatcagttgg taaatgttca aatctgaggg tgtggtcata ttttttaatc ctgaaagata
    32221 agatgtattg accttctatt tttgtgccat aaggatgttg ctggttggtt gctaatatgt
    32281 gaaatagtta atttctacca gctgagacgt tttttcgata acaaggggaa ggtgatggaa
    32341 ctacggcatt tacgctattt catcgcagtg gcgcaggcac tgaactttac ccgtgcggcg
    32401 gaaaaactgc atacctcaca gccttcgtta agcagccaga tccgcgatct tgaaaactgt
    32461 gtcggtgttc cgttactggt gagggataag cgcaaagtcg cgttgacggc ggcgggagaa
    32521 tgttttctcc aggacgcgct ggcaatcctc gaacaagcgg aaaatgccaa attacgggcg
    32581 cggaaaattg ttcaggaaga cagacaatta accattggct ttgtgccatc ggcggaagtg
    32641 aatttattgc caaaagtatt accgatgttt cgtctcagac agccagacac cttgattgag
    32701 ctggtgagtt taatcaccac gcaacaggag gaaaaaattc gccgtggtga actcgacgtc
    32761 ggcttaatgc gccatcctgt ttatagcccg gagattgatt atctggagct ttttgacgaa
    32821 ccattagtgg tggtgttacc ggttgatcac cctttagcac atgaaaaaga gatcaccgcc
    32881 gcgcaactgg atggcgtgaa tttcgtcagt accgatccgg cgtattccgg ttcgcttgcg
    32941 ccgatcgtta aagcgtggtt tgcgcaagaa aacagccagc caaatatcgt ccaggtggca
    33001 acgaatattc tggtgaccat gaatctggtg ggcatggggc tgggcgtcac tttgataccc
    33061 ggttatatga ataattttaa taccgggcag gttgtttttc gtcccattgc cggaaatgtg
    33121 ccttccattg ctttactgat ggcgtggaag aaaggggaga tgaagctggc attacgcgat
    33181 ttcatcgcca ttgtgcagga acgtttggca agcgtaacgg cataattacg cattattttt
    33241 gcaacatgcg tcgcggatcg cagtttttta gtgcttgtga actggcgtta cagacatcgg
    33301 gaagtaggct cgaatattct tatttttcaa cttgcaccaa cattcgatta accccagact
    33361 aacgcctcct gacgggaggg actcatggtt ttgcaatcca cgcgctggtt ggcgctcggc
    33421 tatttcacat acttttttag ttacggcatt tttttacctt tctggagcgt ctggcttaaa
    33481 gggattggtt taacgccaga aaccatcggc ctgttattgg gggcaggtct ggttgcccgt
    33541 tttctcggga gtttgctcat cgcgccccgc gtcagcgatc cttcccgcct gatttccgcc
    33601 ttgcgcgtgc tggcactgct gccacttctc tttgctgtcg ccttctgggc gggggcgcac
    33661 gtagcgtggc tgatgctggt gatgattggc tttaacctct ttttctcacc gctggtaccg
    33721 ttgaccgatg cactggcgaa tacgtggcaa aagcagttcc cgcttgatta cggcaaagtg
    33781 cgactgtggg gctcggtggc gtttgtcatt ggctcggcgc tgacgggcaa actggtcact
    33841 atgtttgatt atcgggtgat cctcgtgctg ttgacgttgg gcgtggcatc catgctgctc
    33901 ggctttctca tccgtccgac gattcagcca caaggggcaa gccgccagca ggagagcacc
    33961 ggttggtctg cgtggttggc gctggttcgc cagaactggc gctttctggc ctgcgtttgt
    34021 ttattgcagg gggcacatgc ggcctattac ggttttagcg ccatttactg gcaggcagct
    34081 ggctactcgg cctcggcggt ggggtatttg tggtcgctgg gcgtggtggc ggaagtcatt
    34141 atctttgcgc tgagtaataa acttttccgc cgttgtagtg ctcgcgatat gctgttgatc
    34201 tcggcgattt gcggcgtagt gcgctggggc attatgggag caactacggc gttgccgtgg
    34261 ttgatagtgg tgcaaattct gcattgcggc accttcacgg tctgccacct ggccgccatg
    34321 cgttatattg ctgctcgcca ggttagcgaa gtcatccgtt tacaggcggt ttactctgcc
    34381 gtcgcgatgg gcggcagtat cgctatcatg accgttttcg ccggtttcct gtatcaatat
    34441 ctgggccacg gcgtgttctg ggtaatggcg ctggtggcgc ttccggcaat gtttttgcgc
    34501 ccgaaagttg ttccctcatg ctgattcgag cattttgcgg atctgctcct gttgatgtgt
    34561 tgtcagcgtc aggtccgtat agatcagcgg tggtgaaaag agcggtaacg gcatggtata
    34621 aggcgcaata atcagtgccg cgccacgcgg agcgcctgtc tgcaaaaatg cctttaccga
    34681 catatgctta atgttgagcg gcagtaacgt tagttcgcgt aactgctgct caatctgcgc
    34741 ttctcgctcg ctatcattcc cggtcagtag aataatctgt ttttcatgca gatcgttttc
    34801 ctgcattagc caggcaccga aaatcaccgc gaccagacca ctttcctcat cagaaagatg
    34861 gacgccgtat tcactttcaa atccggccag cgccgcgcgg gtggtgcgta ccaggcgtgg
    34921 atacagtctg gcgaactctt ccggcagggt attatcaata ccgatggcaa acaaactgcg
    34981 atttaacgcc tgggcgaggt ggatataaag ctgatcacat aacccctgtt catcataaaa
    35041 acgaacattt cccagctcac gaaaatggtt taccagacgt ttgatagact gacgcaattg
    35101 tctgtcccgc tgatgcgcat cccgcagtgg atcgggaacc cgcaacatgg aaaaaagtaa
    35161 tgccataaac agtggctcat caggcggtac atgctggagc gcccgacgct gccagtggcg
    35221 tccaatttcc tgcgctacct gaaattcaag gcaggattcc gcccagcgac gttggagcgg
    35281 attaaactga ggcgtaattc cagcgtggtg ctgcagcaaa caatattgca gatacagaca
    35341 caggaaatga atatcgcgcg attcaaagcg tttttgcagc cgtcgggagc agagattcac
    35401 taacgcttgt agattggtat cgtcgtaaaa gttacgcgca attccgcgcc gcttcagttc
    35461 actcttcagg gcgggggtga attggctggt aataaatgac ggacacagac ggaaaccacg
    35521 tcgtagccag tgaaataaac acaaacgttg gttaagcact gtaccttcaa cccggtagct
    35581 accgtcataa ccagtcgtca gtgtgagttg atgatagcgc aggatctccc gtcctgtttc
    35641 gctgatatca agactggcaa tatcatcatc cacaccatta agctcgctga aggttgccat
    35701 ggtggcggtc aaccccggct gaaagagcgt cagcaagatc tggcagcggc gctggggagc
    35761 cgaaaggaca gatggtggag caagcgtagg catcatctgt agatctccca taagcataat
    35821 tgataagaat agctaaagtt tgctcaggga aatggcgaag ggaatgctaa tcatcagaaa
    35881 tgttggcaag cgtcacagaa tttttctcat tttaaactgt tctgaccagt ggattagcat
    35941 gtcttaaggt ggttttgtga atgattatcg tgctagcgat catgggttag ttctgcggat
    36001 tcaacgcgct aaggataaag tccaccatct gttcgcgata aacattgccg tgacgatcgg
    36061 aaaaagcatc aatgtgttcg ccatccggga ttaggatcaa ccgttttggc tcttttgcca
    36121 ggctatacaa cttttcgctg tgctgccatg ggataacgtg atcggcttta ccgtgaatga
    36181 gtaaaagcgg gatcgggctg acgctggcga tgtaattttc gccgctgtaa ctctcatcaa
    36241 gtaagtagcc actgccgggg atcatctggt tggcgatggt tgcataagag gcaaatgtgg
    36301 agtcgaggat caccgcacgt atgccttcac gatcaccctg accaataaca gcaagaatat
    36361 tcgccccgcc aatactctgc ccgaacagca ccagctgttg tgggtttaca tcactgcgat
    36421 ggcgcaccac attgatggcg ctttgcgtat cgtccagcaa tccggcctgg gacggagtgc
    36481 cttttgattt accaaacccg cgataatcaa acataaaaac gttgaaatta cgctcaggta
    36541 accaactgac cagcggccag tgggcggaca tatttccggc attgccgtga gcatgaatga
    36601 tggttgcgat ggcgttgtca gcagggcccg tcgaagaagg aataaaccag ccttgcagac
    36661 gagtaccgtc cttagccgta aattcgacgg attccgccga ccaggggtcg ggaccgtaaa
    36721 ttttatcgtc aggataatag aagaagttga tggcaacgcg cggcactaaa taaacgcaga
    36781 aggcaacaac aaagagaata aacagaattt tgggaacgcg tttgttcact ggcagtgcca
    36841 taacctttcc ttgcgacttc tttatcatcc attaatcggg gaaagatggt gggagagtga
    36901 cgaaagaaag taaagcggtg aggaagtatt tcggaatagc gcctgaaagg cgagggcggg
    36961 tgagtgatat cacccgcctg agtcattaat gcttcagagc gtcgcttaac tcgtcacgca
    37021 tgttcgccag tatggctttc acaacacgcg ggttaccagc aacgatgtta ccggtcagca
    37081 tatagttatg accaccggtg aagtcgctga cgatgccgcc cgcttcacga accagcagct
    37141 cgcctgccgc gaaatcccac ggacgcagac cgatttcaaa gaaaccgtca acacgacccg
    37201 cagcgacgta agccagatcc agcgccgcag aaccggtacg acggaagtct gcacattcgt
    37261 tgaacagctt gccgacgatg ttgatgtagg tagtggcgta ctgttttgct ttgaacggga
    37321 agccggtcgc cagaatggta ccgtcgagat cgcgagcggt gctaccgcgc agacggtagc
    37381 cgttcagctg tgcgccttga ccgcgagtgg cggtgaacag ttcgttacgc ataggatcgt
    37441 ataccacagc aacttcggtg cggcctttga tacgaacggc gatagatacc gcgaagtgcg
    37501 gcagacgttt gataaagttg gtagtaccat ccagtggatc gataacccat tgaacatcct
    37561 gatcagtacc ttcaagttca ccgctttctt cggtgatgat ggtgtgctgt gggtaagatt
    37621 tacgaatcgt gtcgataatc accgcttcgg cagctttatc tacgttggtc acgaaatcgt
    37681 tactgccttt ctggctcgct tctacagcgt ccggggtttc atagtttttg gcaattaaat
    37741 tacccgcctt gcgcgctgcg cgcacggcga tgttcagcat cggatgcatc ggtctctctc
    37801 actggatgtt aaagaacggg aaaacggcgc agagtatagc agcgagatcg gcatctgtct
    37861 tcgttttatg ataagatgcg tgaataatct ttagatgaag aaagacaatg ctgcaaaata
    37921 ttcgaattgt gctggtggag acgtcacaca ccggcaatat gggttctgtt gcccgtgcca
    37981 tgaaaacaat gggattaacc aatctgtggc tggttaatcc actggtgaaa cccgactccc
    38041 aggcgattgc cctggctgca ggggccagcg atgtgattgg taatgctcac atcgtcgata
    38101 cgctcgacga agcgttagcc ggttgtagcc tggtggtggg caccagcgca cgctcccgca
    38161 cgctgccgtg gccgatgctc gacccgcgcg aatgcggtct gaaaagcgtc gctgaagcgg
    38221 caaatacccc ggtggcgctg gtgtttggtc gtgagcgcgt cggcttgacc aatgaagagt
    38281 tgcagaaatg ccattatcat gtcgcgattg cggcaaaccc ggaatacagt tcgctgaacc
    38341 tggcaatggc ggttcaggtt attgcctatg aagtgcgtat ggcctggctg gcaactcagg
    38401 aaaatggcga gcaggtcgag catgaagaga cgccgtatcc gctggtcgat gatctggagc
    38461 gtttttatgg tcatctggaa caaacgctgc tggcaaccgg ttttatccgt gaaaaccatc
    38521 cggggcaggt gatgaataaa ttgcgccgtc tgtttacccg tgcgcgcccg gaaagccagg
    38581 aattgaatat cctgcgcggg attctggctt ctattgagca gcagaataaa ggtaacaagg
    38641 ccgaataaca gtggttgcct gatgcgacgc ttaacgcgtc ttatcaggcc tacaatgtac
    38701 agaatcgtag gtcggataag gtgttcacac cgcatccgac aacaggtaca aacgccacga
    38761 taaaaaaatg gcactgaagg ttaaataccc gactaaatca gtcaagtaaa tagttgacca
    38821 atttactcgg gaatgtcaga cttgaccctg ctatgcaata cccccacttt tacaataaaa
    38881 aaccccgggc aggggcgagt ttgaggtgaa gtaagacatg agactgacat ctaaagggcg
    38941 ctatgccgtg accgcaatgc ttgacgttgc gctcaactct gaagcgggcc cggtaccgtt
    39001 ggctgatatt tccgaacgtc agggaatttc cctttcttat ctggaacaac tgttttcccg
    39061 tctgcgtaaa aatggtctgg tttccagcgt acgtggacca ggcggtggtt atctgttagg
    39121 caaagatgcc agcagcatcg ccgttggcga agtgattagc gccgttgacg aatctgtaga
    39181 tgccacccgt tgtcagggta aaggcggctg ccagggcggc gataaatgcc tgacccacgc
    39241 gctgtggcgt gatttgagcg accgtctcac cggttttctc aacaacatta ctttaggcga
    39301 actggttaat aaccaggaag tgctggatgt gtctggtcgt cagcatactc acgacgcgcc
    39361 acgcacccgc acacaagacg tgatcgacgt taagttacgc gcttaataaa aagaattcag
    39421 aatcaggccg gagtgctaaa tactccgtaa acacggtcgt acatccagcc ggtagcctga
    39481 ttccttgcat tgagtgatgt acggagttta tagagcaatg aaattaccga tttatctcga
    39541 ctactccgca accacgccgg tggacccgcg tgttgccgag aaaatgatgc agttcatgac
    39601 gatggacgga acctttggta acccggcctc tcgttctcac cgtttcggct ggcaggctga
    39661 agaagcggta gatatcgccc gtaatcagat tgccgatctg gtcggcgctg atccgcgtga
    39721 aatcgtcttt acctctggtg caaccgaatc tgacaacctg gcgatcaaag gtgcagccaa
    39781 cttttatcag aaaaaaggca agcacatcat caccagcaaa accgaacaca aagcggtact
    39841 ggatacttgc cgtcagctgg agcgcgaagg tttcgaagtc acctacctgg caccgcagcg
    39901 taacggtatt atcgacctga aagaactcga agcggcgatg cgtgacgaca ccatccttgt
    39961 ttccatcatg cacgtaaata acgaaatcgg cgtggtgcag gatatcgcgg ctatcggcga
    40021 aatgtgccgt gctcgtggca ttatctatca cgttgatgca acccagagcg tgggcaaact
    40081 gccaatcgac ctgagccagt tgaaagttga cctgatgtct ttctccggtc acaaaatcta
    40141 tggcccgaaa ggtatcggtg cgctgtatgt gcgtcgtaaa ccgcgtgtac gcatcgaagc
    40201 gcaaatgcac ggcggcggtc acgagcgcgg tatgcgttcc ggcactctgc ctgttcacca
    40261 gatcgtcgga atgggcgaag cctatcgcat cgcaaaagaa gagatggcga ccgagatgga
    40321 acgtctgcgc ggcctgcgta accgtctgtg gaacggcatc aaagatatcg aagaagttta
    40381 cctgaacggt gacctggaac acggtgcgcc gaacattctc aacgtcagct tcaactacgt
    40441 tgaaggtgag tcgctgatta tggcgctgaa agacctcgca gtttcttcag gttccgcctg
    40501 tacgtcagca agcctcgaac cgtcctacgt gctgcgcgcg ctggggctga acgacgagct
    40561 ggcacatagc tctatccgtt tctctttagg tcgttttact actgaagaag agatcgacta
    40621 caccatcgag ttagttcgta aatccatcgg tcgtctgcgt gacctttctc cgctgtggga
    40681 aatgtacaag cagggcgtgg atctgaacag catcgaatgg gctcatcatt aatcggtatc
    40741 ggaatcagga gaatttataa tggcttacag cgaaaaagtt atcgaccatt acgagaatcc
    40801 gcgtaacgtg ggttcctttg ataacaacga cgagaacgtc ggcagcggca tggtgggtgc
    40861 accggcctgt ggcgacgtaa tgaagttgca gattaaagtc aacgatgaag gtatcattga
    40921 agacgcgcgt tttaaaactt acggctgcgg ttccgctatc gcttccagct ccctggtcac
    40981 cgaatgggtg aaagggaagt ctctcgacga agcgcaggcg atcaaaaaca ccgatattgc
    41041 tgaagaactt gaactgccgc cggtgaaaat tcactgttct attctggcag aagacgcgat
    41101 caaagccgcc attgcggact ataaaagcaa acgtgaagca aaataagagt tgaggtttgg
    41161 ttatgtcgat tacactgagc gacagtgcag cagcgcgagt aaataccttt ctggctaacc
    41221 gcggcaaagg gtttggcctg cgtctgggcg tgagaacctc cggatgttca ggtatggctt
    41281 atgtactgga atttgttgac gaaccgacgc cggaagacat cgtgtttgaa gacaaaggcg
    41341 tgaaagtcgt ggtcgatggc aaaagcctgc aatttctgga cggtacgcag ctggacttcg
    41401 taaaagaagg cctgaatgaa gggtttaaat tcaccaaccc gaacgttaaa gatgagtgtg
    41461 gttgcggcga aagcttccac gtttgatgcg cataccgata accccaccgt ggtcgcctgc
    41521 gcgtggggtt tgttttacct gattcgccgt tatcgcggcg gatcgcagcc ctgagaatgt
    41581 tatggattac ttcaccctct ttggcttgcc tgcccgttat caactcgata cccaggcact
    41641 gagcctgcgt tttcaggatc tacaacgtca gtatcatcct gataaattcg ccagcggaag
    41701 cctggcggaa caactcgccg ccgtacagca atctgcaacc attaaccagg cctggcaaac
    41761 gctgcgtcat ccgttaatgc gcgcggaata tttgctttct ttacacggtt ttgatctcgc
    41821 cagcgagcag catactgtgc gcgacaccgc gttcctgatg gaacagttgg agctgcgcga
    41881 agagctggac gagatcgaac aggcgaaaga tgaagcgcgg ctggaaagct ttatcaaacg
    41941 tgtgaaaaag atgtttgata cccgccatca gttgatggtt gaacagttag acaacgaggc
    42001 gtgggacgcg gcggcggata cggtgcgtaa gctgcgtttt ctcgataaac tgcggagcag
    42061 tgccgaacaa ctcgaagaaa aactgctcga tttttaattt ctggaagcta aacatggcct
    42121 tattacaaat tagtgaacct ggtttgagtg ctgcgccgca tcagcgtcgt ctggcggctg
    42181 gtattgacct gggcacaacc aactcgctgg tggcgacagt gcgcagcggt caggccgaaa
    42241 cgttagccga ccatgaaggc cgtcacctgc tgccatctgt tgttcactat caacagcaag
    42301 ggcattcggt gggttatgac gcgcgtacta acgcagcgct tgacaccgcc aacaccatta
    42361 gttctgttaa acgcctgatg gggcgctcgc tggctgatat ccagcaacgc tatccgcatc
    42421 tgccttatca attccaggcc agcgaaaacg gcctgccgat gattgaaacg gcggcggggc
    42481 tactgaaccc ggtgcgcgtt tctgcggaca tcctcaaagc actggcggcg cgggcaactg
    42541 aagccctggc aggcgagctg gatggtgtag ttatcaccgt tccggcgtac tttgacgatg
    42601 cccagcgtca gggcaccaaa gacgcggcgc gtctggcggg gttgcatgtt ctgcgcttac
    42661 ttaacgaacc gactgctgcg gctatcgcct acgggctgga ttccggtcag gaaggcgtga
    42721 tcgctgttta tgacctcggt ggcgggacgt ttgatatttc cattctgcgc ttaagtcgcg
    42781 gcgtgtttga agtgctggca accggcggtg attccgcgct cggcggcgat gatttcgacc
    42841 atctgctggc ggattacatt cgcgagcagg cggacattcc tgatcgtagc gataaccgcg
    42901 ttcagcgtga actgctggat gccaccattg cagccaaaat cgcgctgagc gatgcggact
    42961 ccgtgaccgt taacgttgcg ggctggcagg gcgaaatcag ccgtgaacaa ttcaatgaac
    43021 tgatcgcgcc actggtaaaa cgaaccttac tggcttgtcg tcgcgcgctg aaagacgcgg
    43081 gtgtagaagc tgacgaagtg ctggaagtgg tgatggtggg cggttctact cgcgtgccgc
    43141 tggtgcgtga acgggtaggc gaatttttcg gtcgtccacc gctgacttcc atcgacccgg
    43201 ataaagtcgt cgctattggc gcggcgattc aggcggatat tctggtgggt aacaagccag
    43261 acagcgaaat gctgctgctt gatgtgatcc cactgtcgct gggcctcgaa acgatgggcg
    43321 gcctggtgga gaaagtgatt ccgcgtaata ccactattcc ggtggcccgc gctcaggatt
    43381 tcaccacctt taaagatggt cagacggcga tgtctatcca tgtaatgcag ggtgagcgcg
    43441 aactggtgca ggactgccgc tcactggcgc gttttgcgct gcgtggtatt ccggcgctac
    43501 cggctggcgg tgcgcatatt cgcgtgacgt tccaggtcga tgccgacggt cttttgagcg
    43561 tgacggcgat ggagaaatcc accggcgttg aggcgtctat tcaggtcaaa ccgtcttacg
    43621 gtctgaccga tagcgaaatc gcttcgatga tcaaagactc aatgagctat gccgagcagg
    43681 acgtaaaagc ccgtatgctg gcagaacaaa aagtagaagc ggcgcgtgtg ctggaaagtc
    43741 tgcacggcgc gctggctgct gatgccgcgc tgttaagcgc cgcagagcgt caggtcattg
    43801 acaatgctgc cgctcacctg agtgaagtgg cgcagggcga tgatgttgac gccatcgaac
    43861 aagcgattaa aaacgtagac aaacaaaccc aggatttcgc cgctcgccgc atggaccagt
    43921 cggttcgtcg tgcgctgaaa ggccattccg tggacgaggt ttaatatgcc aaagattgtt
    43981 attttgcctc atcaggacct ctgccctgat ggcgctgttc tggaagctaa tagcggtgaa
    44041 accattctcg acgcagcgct gcgtaacggt attgagattg aacacgcctg tgaaaaatcc
    44101 tgtgcttgca ccacctgcca ctgcatcgtt cgtgaaggtt ttgactcact gccggaaagc
    44161 tcagagcagg aagacgacat gctggacaaa gcctggggac tggagccgga aagccgttta
    44221 agctgccagg cgcgcgtcac cgacgaagat ttagtggtcg aaatcccgcg ttacactatc
    44281 aaccatgcgc gtgagcatta acagaggtta gtatgggact taagtggacc gatagccgcg
    44341 aaattggcga agcactgtac gatgcgtatc ccgatcttga tccgaaaacg gttcgattca
    44401 ccgatatgca tcagtggatt tgcgatctgg aagatttcga cgacgacccg caggcatcca
    44461 acgagaaaat cctcgaagcg attttgttag tctggctgga cgaagccgag tgaatgaacg
    44521 ggctgcctgg gggtagcccg ttttgctgtt aaacgaataa ggataactaa aatgacagaa
    44581 gcgatgaaga ttaccctctc tacccaacct gccgacgcgc gctggggaga aaaagcaact
    44641 tacagcatta ataatgacgg cattaccctg catttgaacg gggcagacga tctggggctg
    44701 atccagcgtg cggcccgcaa gattgacggt ctgggcatca agcatgttca gttaagcggt
    44761 gaaggctggg atgcggatcg ctgctgggca ttctggcaag gttataaagg cccgaaaggc
    44821 acgcgtaaag tggagtggcc ggatctggac gatgcccagc gccaggaact ggataaccgc
    44881 ctgatgatca tcgactgggt gcgtgacacc atcaacgcac cggcagaaga attgggacca
    44941 tcgcaactgg cacagcgtgc tgttgatctg atcagcaacg tcgcgggcga tcgtgtgact
    45001 tatcggatca ccaaaggcga agatctgcgt gagcaaggtt atatggggct gcacactgtc
    45061 gggcgcggtt cagaacgttc tccggtattg ctggcgctgg attacaaccc aactggcgat
    45121 aaagaagcac cggtgtacgc gtgcctggta ggtaaaggta tcacttttga ctccggcggc
    45181 tacagcatca aacagactgc gtttatggac tcgatgaagt cggacatggg cggcgcggca
    45241 acggttaccg gggcgctggc atttgccatt atgcgcggac tgaacaagcg cgtgaagctg
    45301 ttcctctgct gtgcggataa cctgattagc ggcaatgcgt tcaagctggg cgatatcatc
    45361 acctatcgca acggtaaaaa agttgaagtg atgaacactg atgccgaagg gcgtctggtg
    45421 cttgccgatg gtctgattga tgccagtgcg cagaaaccgg aaatgatcat tgatgcggcg
    45481 accctcaccg gggcggcgaa aactgcgctg ggtaatgatt atcacgcgct gttcagtttt
    45541 gacgatgcgc ttgccggtcg tttactggcg agtgcctcac aagagaacga accattctgg
    45601 cgtctgccgc tggcggaatt ccaccgcagc cagctgccgt ctaactttgc cgaactgaac
    45661 aataccggaa gcgcggcgta tccggcaggc gcgagcacgg cggcgggctt cctgtcgcac
    45721 tttgttgaga actatcagca aggctggctg catatcgact gctcggcgac ttaccgtaaa
    45781 gcgccggttg aacagtggtc tgcgggcgct acgggacttg gtgtgcgcac gatagctaat
    45841 ctgttaacgg cgtaaactaa tatgccggat gcggcgtaaa cgccttatcc ggcctacaaa
    45901 ttctgcaaat tcaacaaatt gcgaatccct tgtaggcctg ataagcgtag cgcatcaggc
    45961 atttgcgccc cgaacgttga atgaattgtg gttcttatgt ccgaaacaaa aaacgaactt
    46021 gaagacctgc tggaaaaagc agcaaccgaa ccggcgcacc gcccggcctt tttccgtact
    46081 ctactggaat ccaccgtctg ggtgcctggt acggcggcgc agggcgaggc tgtggttgaa
    46141 gatagtgcgc ttgatttaca gcactgggaa aaagaagacg gcaccagcgt cattcctttt
    46201 ttcacctcgt tagaagcgct tcaacaggcg gttgaagacg aacaggcatt tgtcgtaatg
    46261 cccgttcgca cattgtttga gatgaccctt ggcgaaacgc tcttccttaa tgccaaactg
    46321 ccgaccggta aagaatttat gccgcgtgaa atcagtttgt tgattggtga agagggaaat
    46381 ccgctgagca gccaggaaat cctggaaggc ggtgaatcgc tgatattatc ggaagtcgca
    46441 gagccgccag cacaaatgat tgattcactc accaccttat ttaaaaccat taagccggtg
    46501 aagcgtgctt ttatttgttc aattaaagag aacgaagagg cacagcctaa tttacttatt
    46561 ggcattgaag ccgatggtga tatcgaagaa attattcagg cgacgggaag tgtagcgacc
    46621 gatacattac ctggcgatga accaatcgat atttgtcagg tgaaaaaagg ggaaaaagga
    46681 attagccact ttattaccga acatattgcg ccattctatg aacgtcgctg gggtggtttt
    46741 ttgcgtgact ttaaacaaaa ccggataatc taaaatatgc cgccctaaag ggcggcattc
    46801 gggcttactt gctgctctga aggttgttaa ccaccagtaa attgtcgccg gagagactcc
    46861 ggttattatt tacttgggaa aacagcgcac cagaaacaat aagtacgata cagaagcacc
    46921 gtatcttcat tgtgcggtcc atgcgaagac cgcatcacga cagcaccact ggcgatattg
    46981 ccgcgatagc aagcgctgag acgcgcttgc tctaacggtc gcggaacatc ctttccttaa
    47041 gccagcgccc gcttgcgaga cggcggaaag ggccgcagtg gataatacac taacagaaca
    47101 ataataactc tcttttgctt gacaaaaaga gagttactgg cgagtattgt ctccgctgag
    47161 acgcgcttgc attactacgg taatacaacc tgctggaaag gccgcgaacc ggaccagcaa
    47221 caaataaccg cctgattttg gcggtttttt gttttttaaa ggaaagtaaa tttacatgtt
    47281 taagtaatat cttttaccgg ttagaatttt taattttgag caggatttgt ttatatttgt
    47341 ctgaattcgg actaataagc caggttattt tattaccgaa cacggcggaa agggccggtg
    47401 tcgataatac gctaaataaa caataattac tctcttttgc ttgacaaaaa gagagttact
    47461 ggtgagtatt gttttgctgt gaagcacctg cgttgctcat gtaacacaag tcaacctgct
    47521 ggaaaggccg cgaaccagac cagcaataaa aaaacgccaa attcggcggt tttttattgc
    47581 ctgtaaaata cttatttcac tggctcaacc ggtaaatctg cccgcgcgcc ccattcactc
    47641 catgcgccgt cgtacagttt cacgtttggc acatccagcg tcgcgagtgc taacaaaacc
    47701 acggctgccg ttacaccaga gccgcagctg acgataatcg gtttgtcata gctgacgccg
    47761 cgaccaaaaa atatcgcatc cagttcatcg gtcgttttta gttcgccttc gcgcaccagt
    47821 tccgtccacg gaacgttcaa tgcaccggga atatgtccgc gacgtaaacc tgggcgaggt
    47881 tcatcaactt ctgcgttaaa acgtgcagcc gggcgggcat caataatttg cgccgtattt
    47941 tcatggcttg ccaataacac atcggttact ttcaccactg cttcaggatt aaacgcggcg
    48001 ttaaactctc cttccggcag ctctactgca ccttcttcta atagcaggtc atcgcgctgc
    48061 cagcctgcaa g
//
LOCUS       DAOFMX010000024        47955 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.024, whole genome shotgun sequence.
ACCESSION   DAOFMX010000024 DAOFMX010000000
VERSION     DAOFMX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.024"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="QBA20_001531"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="QBA20_001531"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDQ6292116.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="QBA20_001532"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="QBA20_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDQ6292117.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="QBA20_001533"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="QBA20_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDQ6292118.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="QBA20_001534"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="QBA20_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDQ6292119.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="QBA20_001535"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="QBA20_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDQ6292120.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="QBA20_001536"
     CDS             5377..5835
                     /locus_tag="QBA20_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDQ6292121.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="QBA20_001537"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="QBA20_001537"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDQ6292122.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="QBA20_001538"
     tRNA            6887..6971
                     /locus_tag="QBA20_001538"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="QBA20_001539"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="QBA20_001539"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="QBA20_001540"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="QBA20_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDQ6292123.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="QBA20_001541"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="QBA20_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDQ6292124.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="QBA20_001542"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="QBA20_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDQ6292125.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="QBA20_001543"
     CDS             complement(10252..13995)
                     /locus_tag="QBA20_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDQ6292126.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="QBA20_001544"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="QBA20_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDQ6292127.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="QBA20_001545"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="QBA20_001545"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDQ6292128.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="QBA20_001546"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="QBA20_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDQ6292129.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPNLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="QBA20_001547"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="QBA20_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDQ6292130.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="QBA20_001548"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="QBA20_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDQ6292131.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="QBA20_001549"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="QBA20_001549"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDQ6292132.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="QBA20_001550"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="QBA20_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDQ6292133.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="QBA20_001551"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="QBA20_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDQ6292134.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="QBA20_001552"
     CDS             23392..23499
                     /locus_tag="QBA20_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDQ6292135.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="QBA20_001553"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="QBA20_001553"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDQ6292136.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="QBA20_001554"
     CDS             complement(24538..25347)
                     /locus_tag="QBA20_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDQ6292137.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="QBA20_001555"
     CDS             complement(25351..25743)
                     /locus_tag="QBA20_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDQ6292138.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="QBA20_001556"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="QBA20_001556"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDQ6292139.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="QBA20_001557"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="QBA20_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDQ6292140.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="QBA20_001558"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="QBA20_001558"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDQ6292141.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="QBA20_001559"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="QBA20_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDQ6292142.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="QBA20_001560"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="QBA20_001560"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDQ6292143.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="QBA20_001561"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="QBA20_001561"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDQ6292144.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="QBA20_001562"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="QBA20_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDQ6292145.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="QBA20_001563"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="QBA20_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDQ6292146.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="QBA20_001564"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="QBA20_001564"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDQ6292147.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="QBA20_001565"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="QBA20_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDQ6292148.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="QBA20_001566"
     CDS             36790..39657
                     /locus_tag="QBA20_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ6292149.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="QBA20_001567"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="QBA20_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDQ6292150.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="QBA20_001568"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="QBA20_001568"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDQ6292151.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="QBA20_001569"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="QBA20_001569"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDQ6292152.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="QBA20_001570"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="QBA20_001570"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDQ6292153.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="QBA20_001571"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="QBA20_001571"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDQ6292154.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="QBA20_001572"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="QBA20_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12034 a        12346 c        11746 g        11829 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgccgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcagcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggca cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc gcggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccaa gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagaggcagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcattcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct gtagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggt ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccaatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatcgt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacactgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccgcgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgcttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggagg
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgcctgttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtcgg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga attagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgccaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccga acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacagtta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaagtcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tggtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccgg
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgcattaat aaaat
//
LOCUS       DAOFMX010000025        45838 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.025, whole genome shotgun sequence.
ACCESSION   DAOFMX010000025 DAOFMX010000000
VERSION     DAOFMX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.025"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            95..2248
                     /locus_tag="QBA20_001573"
     CDS             95..2248
                     /locus_tag="QBA20_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HDQ6292155.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="QBA20_001574"
     CDS             2301..4031
                     /locus_tag="QBA20_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292156.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="QBA20_001575"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="QBA20_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDQ6292157.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="QBA20_001576"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="QBA20_001576"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDQ6292158.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="QBA20_001577"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="QBA20_001577"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDQ6292159.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="QBA20_001578"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="QBA20_001578"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDQ6292160.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="QBA20_001579"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="QBA20_001579"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDQ6292161.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="QBA20_001580"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="QBA20_001580"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDQ6292162.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="QBA20_001581"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="QBA20_001581"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDQ6292163.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="QBA20_001582"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="QBA20_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDQ6292164.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="QBA20_001583"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="QBA20_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDQ6292165.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="QBA20_001584"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="QBA20_001584"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDQ6292166.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="QBA20_001585"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="QBA20_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDQ6292167.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="QBA20_001586"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="QBA20_001586"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDQ6292168.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="QBA20_001587"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="QBA20_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDQ6292169.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="QBA20_001588"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="QBA20_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDQ6292170.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="QBA20_001589"
     CDS             16289..16828
                     /locus_tag="QBA20_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDQ6292171.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /gene="tsx"
                     /locus_tag="QBA20_001590"
     CDS             17126..18010
                     /gene="tsx"
                     /locus_tag="QBA20_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDQ6292172.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /locus_tag="QBA20_001591"
     CDS             complement(18187..18534)
                     /locus_tag="QBA20_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDQ6292173.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="QBA20_001592"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="QBA20_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDQ6292174.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEEPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..21492)
                     /gene="secD"
                     /locus_tag="QBA20_001593"
     CDS             complement(19645..21492)
                     /gene="secD"
                     /locus_tag="QBA20_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDQ6292175.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="QBA20_001594"
     CDS             complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="QBA20_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDQ6292176.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="QBA20_001595"
     CDS             complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="QBA20_001595"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDQ6292177.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23057..24127)
                     /gene="queA"
                     /locus_tag="QBA20_001596"
     CDS             complement(23057..24127)
                     /gene="queA"
                     /locus_tag="QBA20_001596"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDQ6292178.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24220..24793
                     /gene="acpH"
                     /locus_tag="QBA20_001597"
                     /pseudo
     CDS             24220..24793
                     /gene="acpH"
                     /locus_tag="QBA20_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="QBA20_001598"
     CDS             complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="QBA20_001598"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDQ6292179.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26771..28144)
                     /gene="proY"
                     /locus_tag="QBA20_001599"
     CDS             complement(26771..28144)
                     /gene="proY"
                     /locus_tag="QBA20_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDQ6292180.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="QBA20_001600"
     CDS             complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="QBA20_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDQ6292181.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="QBA20_001601"
     CDS             complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="QBA20_001601"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDQ6292182.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="QBA20_001602"
     CDS             complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="QBA20_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDQ6292183.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32178..33380
                     /gene="sbcD"
                     /locus_tag="QBA20_001603"
     CDS             32178..33380
                     /gene="sbcD"
                     /locus_tag="QBA20_001603"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDQ6292184.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSCEQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33377..36520
                     /gene="sbcC"
                     /locus_tag="QBA20_001604"
     CDS             33377..36520
                     /gene="sbcC"
                     /locus_tag="QBA20_001604"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDQ6292185.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEKLTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36646..37827
                     /gene="araJ"
                     /locus_tag="QBA20_001605"
     CDS             36646..37827
                     /gene="araJ"
                     /locus_tag="QBA20_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDQ6292186.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37970..38878)
                     /gene="mak"
                     /locus_tag="QBA20_001606"
     CDS             complement(37970..38878)
                     /gene="mak"
                     /locus_tag="QBA20_001606"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDQ6292187.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39003..39914
                     /gene="rdgC"
                     /locus_tag="QBA20_001607"
     CDS             39003..39914
                     /gene="rdgC"
                     /locus_tag="QBA20_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDQ6292188.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39992..40447)
                     /locus_tag="QBA20_001608"
     CDS             complement(39992..40447)
                     /locus_tag="QBA20_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292189.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40444..40779)
                     /locus_tag="QBA20_001609"
                     /pseudo
     CDS             complement(40444..40779)
                     /locus_tag="QBA20_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="QBA20_001610"
     CDS             complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="QBA20_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDQ6292190.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41290..41967)
                     /locus_tag="QBA20_001611"
     CDS             complement(41290..41967)
                     /locus_tag="QBA20_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDQ6292191.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42228..42416)
                     /gene="yaiA"
                     /locus_tag="QBA20_001612"
     CDS             complement(42228..42416)
                     /gene="yaiA"
                     /locus_tag="QBA20_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDQ6292192.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="QBA20_001613"
     CDS             complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="QBA20_001613"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDQ6292193.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43173..43631)
                     /locus_tag="QBA20_001614"
     CDS             complement(43173..43631)
                     /locus_tag="QBA20_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDQ6292194.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43751..44560
                     /gene="proC"
                     /locus_tag="QBA20_001615"
     CDS             43751..44560
                     /gene="proC"
                     /locus_tag="QBA20_001615"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDQ6292195.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="QBA20_001616"
                     /gene_synonym="dgcC"
     CDS             complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="QBA20_001616"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDQ6292196.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11912 a        11952 c        11216 g        10758 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatctacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttatga tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgtgcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc aacaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggctccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaattttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggttcttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    21181 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    21241 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    21301 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    21361 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    21421 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    21481 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    21541 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    21601 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    21661 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    21721 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21781 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21841 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21901 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21961 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    22021 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    22081 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    22141 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    22201 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt
    22261 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    22321 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    22381 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    22441 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    22501 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    22561 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    22621 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    22681 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    22741 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22801 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22861 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22921 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22981 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    23041 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    23101 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    23161 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    23221 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    23281 agatttgggt atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc
    23341 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    23401 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    23461 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    23521 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    23581 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    23641 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    23701 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    23761 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23821 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23881 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23941 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    24001 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    24061 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    24121 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    24181 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    24241 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    24301 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    24361 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    24421 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    24481 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    24541 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    24601 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    24661 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    24721 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24781 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24841 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24901 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24961 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    25021 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    25081 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    25141 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    25201 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    25261 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    25321 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    25381 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    25441 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    25501 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    25561 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    25621 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    25681 tcattcacca gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag
    25741 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg
    25801 tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg
    25861 tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa
    25921 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    25981 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc
    26041 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    26101 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    26161 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    26221 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca
    26281 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    26341 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    26401 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
    26461 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    26521 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    26581 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    26641 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    26701 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    26761 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    26821 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    26881 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    26941 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    27001 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    27061 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    27121 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    27181 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    27241 cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg
    27301 caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct
    27361 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
    27421 gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg
    27481 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    27541 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    27601 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    27661 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    27721 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    27781 gcaccgtcgg gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    27841 caataaggat ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt
    27901 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    27961 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    28021 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    28081 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt
    28141 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    28201 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    28261 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    28321 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    28381 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    28441 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    28501 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca
    28561 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    28621 caaagaattc tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga
    28681 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    28741 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga
    28801 acagcgccag gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat
    28861 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    28921 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    28981 aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    29041 tcgcgacaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    29101 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    29161 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    29221 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    29281 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    29341 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    29401 cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc gaccattgga gggaaaataa
    29461 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    29521 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    29581 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    29641 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    29701 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    29761 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    29821 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    29881 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    29941 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    30001 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    30061 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    30121 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    30181 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    30241 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    30301 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    30361 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    30421 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    30481 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    30541 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    30601 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    30661 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    30721 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    30781 tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag
    30841 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    30901 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    30961 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    31021 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
    31081 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
    31141 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    31201 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    31261 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    31321 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    31381 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    31441 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    31501 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    31561 gatgtcgggt cgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa
    31621 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg
    31681 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    31741 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    31801 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    31861 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    31921 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    31981 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    32041 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    32101 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt
    32161 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    32221 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    32281 cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    32341 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    32401 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    32461 atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    32521 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc
    32581 gtgacattat ttccagccag gcggggctta acggtattga aaaacagcag catttactgg
    32641 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    32701 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg
    32761 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    32821 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    32881 ttcgctattg cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg
    32941 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    33001 taactcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    33061 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    33121 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    33181 tattgctggt acgtcggagt tgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    33241 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac
    33301 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg
    33361 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    33421 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    33481 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    33541 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    33601 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    33661 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga
    33721 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    33781 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    33841 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagaa
    33901 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    33961 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc
    34021 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    34081 aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    34141 gcagcaagaa gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa
    34201 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    34261 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga
    34321 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    34381 gaagcagtca gcagaattac agcagcagca acaaagccta aatacctggt tacaggaaca
    34441 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    34501 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    34561 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    34621 agcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    34681 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    34741 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    34801 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    34861 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc
    34921 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    34981 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    35041 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    35101 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    35161 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    35221 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    35281 tcaacagcaa attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact
    35341 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    35401 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    35461 accgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    35521 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    35581 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga
    35641 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    35701 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    35761 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    35821 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    35881 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    35941 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    36001 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    36061 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    36121 ccggctgcac gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    36181 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    36241 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    36301 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    36361 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    36421 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    36481 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    36541 tacagagggg tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    36601 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    36661 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg
    36721 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    36781 tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    36841 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    36901 acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat
    36961 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    37021 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    37081 ctgggaacgt atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt
    37141 aatattgcgg tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa
    37201 ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc
    37261 gccacgatgt ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg
    37321 atgtttattt ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta
    37381 gggatggtgc tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc
    37441 attgcagcag tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc
    37501 ggcatgaaaa caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt
    37561 tcagcacctc tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc
    37621 gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg
    37681 atgctgacgc tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt
    37741 gctgcgatgt catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt
    37801 ccggtgctgg cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg
    37861 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa
    37921 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    37981 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg
    38041 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata
    38101 aacggtctac attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca
    38161 cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta
    38221 ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca
    38281 aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt
    38341 taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta
    38401 gcggattgtg tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga
    38461 atgccacgcc cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg
    38521 ctgccgcgcc atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca
    38581 cttcccgctg caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg
    38641 aattgacatt cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta
    38701 ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa
    38761 tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg
    38821 catcgcccag tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg
    38881 tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc
    38941 aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta
    39001 tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg
    39061 cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca
    39121 tggcgaagat gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg
    39181 ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga
    39241 ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga
    39301 agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt
    39361 tcagccgttt tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg
    39421 actgcgccag tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt
    39481 cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg
    39541 ttcgctccgg tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt
    39601 tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca
    39661 ccaatcacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca
    39721 ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc
    39781 gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca
    39841 tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg
    39901 aagcacaacg ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta
    39961 tccggcgtac gaagccacac caggcatata attattcgct acggcgagca ataattttta
    40021 gcgcagcaat attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc
    40081 tgtcagcagc acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta
    40141 acagctccgc ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat
    40201 ccgtcaggta aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg
    40261 caataaagtc tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg
    40321 gtaaatcggt acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca
    40381 ggttgatcgt tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt
    40441 gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc
    40501 ggttttcact aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga
    40561 tggatcctct tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc
    40621 cagcgaccga tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt
    40681 gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc
    40741 atcatctgat ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact
    40801 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
    40861 aggaaatatt attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg
    40921 ggaaggcgag gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat
    40981 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
    41041 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    41101 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    41161 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    41221 actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt
    41281 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
    41341 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    41401 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt
    41461 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    41521 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    41581 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    41641 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    41701 ctgattatcg agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac
    41761 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    41821 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    41881 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    41941 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    42001 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    42061 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg
    42121 ttattgttta atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc
    42181 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcacta tttgtcaggg
    42241 tcctcatagc gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac
    42301 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    42361 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    42421 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    42481 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    42541 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    42601 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    42661 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    42721 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc
    42781 tgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    42841 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
    42901 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    42961 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    43021 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    43081 aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata
    43141 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    43201 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    43261 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
    43321 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc
    43381 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    43441 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
    43501 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    43561 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    43621 caaattgtca taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt
    43681 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    43741 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    43801 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc
    43861 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    43921 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
    43981 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    44041 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    44101 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    44161 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    44221 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
    44281 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    44341 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
    44401 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    44461 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    44521 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    44581 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    44641 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    44701 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    44761 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    44821 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    44881 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    44941 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    45001 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga
    45061 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    45121 acccagccaa acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    45181 ggagcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    45241 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    45301 cacagaatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    45361 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    45421 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
    45481 agccaccacc agcccggcgg cgggtgcgaa accagcgttg aagcaatcgg taagaacatg
    45541 ccagccaggc caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
    45601 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa
    45661 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
    45721 acggggctaa aggcagaagt atgaaatttc gggtgagcca ctttcgtagc tcacccgtta
    45781 gcactatctg aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctg
//
LOCUS       DAOFMX010000026        44591 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.026, whole genome shotgun sequence.
ACCESSION   DAOFMX010000026 DAOFMX010000000
VERSION     DAOFMX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.026"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..358
                     /locus_tag="QBA20_001617"
     CDS             <1..358
                     /locus_tag="QBA20_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDQ6292197.1"
                     /translation="NYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILK
                     NELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNV
                     SSGQIMSRVKIVVMYN"
     gene            complement(396..1295)
                     /gene="nhaR"
                     /locus_tag="QBA20_001618"
     CDS             complement(396..1295)
                     /gene="nhaR"
                     /locus_tag="QBA20_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDQ6292198.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVTDA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1361..2527)
                     /gene="nhaA"
                     /locus_tag="QBA20_001619"
     CDS             complement(1361..2527)
                     /gene="nhaA"
                     /locus_tag="QBA20_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDQ6292199.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3113..3265
                     /gene="mokC"
                     /locus_tag="QBA20_001620"
     CDS             3113..3265
                     /gene="mokC"
                     /locus_tag="QBA20_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDQ6292200.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3369..4499)
                     /gene="dnaJ"
                     /locus_tag="QBA20_001621"
     CDS             complement(3369..4499)
                     /gene="dnaJ"
                     /locus_tag="QBA20_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDQ6292201.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4588..6504)
                     /gene="dnaK"
                     /locus_tag="QBA20_001622"
     CDS             complement(4588..6504)
                     /gene="dnaK"
                     /locus_tag="QBA20_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDQ6292202.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6881..7285
                     /locus_tag="QBA20_001623"
     CDS             6881..7285
                     /locus_tag="QBA20_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDQ6292203.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7311..8024
                     /gene="msyB"
                     /locus_tag="QBA20_001624"
     CDS             7311..8024
                     /gene="msyB"
                     /locus_tag="QBA20_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDQ6292204.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8173..8739
                     /gene="satP"
                     /locus_tag="QBA20_001625"
     CDS             8173..8739
                     /gene="satP"
                     /locus_tag="QBA20_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDQ6292205.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8774..9361)
                     /gene="mog"
                     /locus_tag="QBA20_001626"
     CDS             complement(8774..9361)
                     /gene="mog"
                     /locus_tag="QBA20_001626"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDQ6292206.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9476..10429)
                     /gene="tal"
                     /locus_tag="QBA20_001627"
     CDS             complement(9476..10429)
                     /gene="tal"
                     /locus_tag="QBA20_001627"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDQ6292207.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10708..12138
                     /locus_tag="QBA20_001628"
                     /pseudo
     CDS             10708..12138
                     /locus_tag="QBA20_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12208..12984
                     /gene="yaaA"
                     /locus_tag="QBA20_001629"
     CDS             12208..12984
                     /gene="yaaA"
                     /locus_tag="QBA20_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDQ6292208.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13137..13433)
                     /locus_tag="QBA20_001630"
     CDS             complement(13137..13433)
                     /locus_tag="QBA20_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDQ6292209.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13647..14933)
                     /gene="thrC"
                     /locus_tag="QBA20_001631"
     CDS             complement(13647..14933)
                     /gene="thrC"
                     /locus_tag="QBA20_001631"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDQ6292210.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14934..15866)
                     /gene="thrB"
                     /locus_tag="QBA20_001632"
     CDS             complement(14934..15866)
                     /gene="thrB"
                     /locus_tag="QBA20_001632"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDQ6292211.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15868..18330)
                     /gene="thrA"
                     /locus_tag="QBA20_001633"
     CDS             complement(15868..18330)
                     /gene="thrA"
                     /locus_tag="QBA20_001633"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDQ6292212.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18356..18481)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18411..18482)
                     /gene="thrL"
                     /locus_tag="QBA20_001634"
     CDS             complement(18411..18482)
                     /gene="thrL"
                     /locus_tag="QBA20_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDQ6292213.1"
                     /translation="MKRISTTITTTITITITTGNGAG"
     gene            complement(18696..19382)
                     /locus_tag="QBA20_001635"
     CDS             complement(18696..19382)
                     /locus_tag="QBA20_001635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDQ6292214.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19782..19922)
                     /locus_tag="QBA20_001636"
     CDS             complement(19782..19922)
                     /locus_tag="QBA20_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292215.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDSTVSKTAVILTILT
                     AT"
     gene            20018..20734
                     /gene="arcA"
                     /locus_tag="QBA20_001637"
     CDS             20018..20734
                     /gene="arcA"
                     /locus_tag="QBA20_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDQ6292216.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20793..22145)
                     /gene="creD"
                     /locus_tag="QBA20_001638"
     CDS             complement(20793..22145)
                     /gene="creD"
                     /locus_tag="QBA20_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDQ6292217.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22203..23627)
                     /gene="creC"
                     /locus_tag="QBA20_001639"
     CDS             complement(22203..23627)
                     /gene="creC"
                     /locus_tag="QBA20_001639"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDQ6292218.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23627..24316)
                     /gene="creB"
                     /locus_tag="QBA20_001640"
                     /pseudo
     CDS             complement(23627..24316)
                     /gene="creB"
                     /locus_tag="QBA20_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
     gene            complement(24329..24802)
                     /gene="creA"
                     /locus_tag="QBA20_001641"
     CDS             complement(24329..24802)
                     /gene="creA"
                     /locus_tag="QBA20_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDQ6292219.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25013..25882
                     /gene="robA"
                     /locus_tag="QBA20_001642"
     CDS             25013..25882
                     /gene="robA"
                     /locus_tag="QBA20_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDQ6292220.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25879..26526)
                     /gene="gpmB"
                     /locus_tag="QBA20_001643"
     CDS             complement(25879..26526)
                     /gene="gpmB"
                     /locus_tag="QBA20_001643"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDQ6292221.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26578..27098
                     /gene="yjjX"
                     /locus_tag="QBA20_001644"
                     /pseudo
     CDS             26578..27098
                     /gene="yjjX"
                     /locus_tag="QBA20_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27183..27509)
                     /gene="trpR"
                     /locus_tag="QBA20_001645"
     CDS             complement(27183..27509)
                     /gene="trpR"
                     /locus_tag="QBA20_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDQ6292222.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27599..29536)
                     /gene="sltY"
                     /locus_tag="QBA20_001646"
     CDS             complement(27599..29536)
                     /gene="sltY"
                     /locus_tag="QBA20_001646"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDQ6292223.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGHSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29747..31414
                     /gene="ettA"
                     /locus_tag="QBA20_001647"
     CDS             29747..31414
                     /gene="ettA"
                     /locus_tag="QBA20_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDQ6292224.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31521..31688)
                     /locus_tag="QBA20_001648"
     CDS             complement(31521..31688)
                     /locus_tag="QBA20_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292225.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31722..32954)
                     /gene="nadR"
                     /locus_tag="QBA20_001649"
     CDS             complement(31722..32954)
                     /gene="nadR"
                     /locus_tag="QBA20_001649"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDQ6292226.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32975..34357)
                     /gene="radA"
                     /locus_tag="QBA20_001650"
     CDS             complement(32975..34357)
                     /gene="radA"
                     /locus_tag="QBA20_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDQ6292227.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34406..35374)
                     /gene="serB"
                     /locus_tag="QBA20_001651"
     CDS             complement(34406..35374)
                     /gene="serB"
                     /locus_tag="QBA20_001651"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDQ6292228.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLLLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35480..36124
                     /locus_tag="QBA20_001652"
     CDS             35480..36124
                     /locus_tag="QBA20_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDQ6292229.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36152..37167
                     /gene="lplA"
                     /locus_tag="QBA20_001653"
                     /pseudo
     CDS             36152..37167
                     /gene="lplA"
                     /locus_tag="QBA20_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37199..37462
                     /locus_tag="QBA20_001654"
     CDS             37199..37462
                     /locus_tag="QBA20_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6292230.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37623..38342)
                     /gene="deoD"
                     /locus_tag="QBA20_001655"
     CDS             complement(37623..38342)
                     /gene="deoD"
                     /locus_tag="QBA20_001655"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDQ6292231.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38399..39622)
                     /gene="deoB"
                     /locus_tag="QBA20_001656"
     CDS             complement(38399..39622)
                     /gene="deoB"
                     /locus_tag="QBA20_001656"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDQ6292232.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39674..40996)
                     /gene="deoA"
                     /locus_tag="QBA20_001657"
     CDS             complement(39674..40996)
                     /gene="deoA"
                     /locus_tag="QBA20_001657"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDQ6292233.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41123..41902)
                     /gene="deoC"
                     /locus_tag="QBA20_001658"
     CDS             complement(41123..41902)
                     /gene="deoC"
                     /locus_tag="QBA20_001658"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDQ6292234.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42160..43701
                     /locus_tag="QBA20_001659"
     CDS             42160..43701
                     /locus_tag="QBA20_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDQ6292235.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43673..44536
                     /locus_tag="QBA20_001660"
     CDS             43673..44536
                     /locus_tag="QBA20_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDQ6292236.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT        10667 a        12063 c        11123 g        10738 t
ORIGIN      
        1 aaattatcag tttcatcata taagatttga ctgcatccag ggcgagcaag gtgaattagt
       61 ttttgaaccc agtgacgttg aatattactt tgaaccagtg acgatacagg agtcaggaac
      121 cagcatatta aaaaatgagt tagaaaacag tgaggacggt gcgggtcaaa taggctttca
      181 actctccaat gatggtacga atgaaatcca gtatggtaaa agcaattact actcttttca
      241 gcatccccat gaaggaagta atcagattcc tctatttatt cgcccgcgta catatggaaa
      301 caatgtctca agcgggcaaa taatgagccg agtcaaaatc gtggtaatgt acaactaatc
      361 tatctccccc accttaactt tgggggagct gccgattaac gcaccgctgg actaaaaagc
      421 gcagaataat ccgtattgca gattcgctgt accgccgggt gctgaatcat ccgctcagca
      481 aaaatagcat ggtactcttc catcacattc tcgacgcgac caatttctac gacagtttta
      541 tcggcataaa agtcatatgc ataaagcgtt ggggcaacga agattgcatt gtgcatcgca
      601 ccaaaagctt tcatcaaagc ggcatcatca aactcgccga ggatttctac gtttaatccc
      661 tgggagttaa accagttaag caatttgcgc cctaacattg aacgtcgccc aggaatcaaa
      721 agtcgccgtt cttccagaca agccgggaac ggtttttctg gtggtggatt tgtacaccag
      781 aaactcacgc cacattcgcc aattctcacg gagaacaggc cttcctgctg cgtagagtct
      841 atcggacagt cagaaatgat catatccagt ttatgctgac ttaattgctc cagcagcatt
      901 tcgtgggtgg attcgaagca gcgaagatga atgggctcgc cttctaccac tgcggcgtta
      961 agtacgctac tgaccaggcg tttggaaagt gcatcagtca cgccaacgtc aaacaataaa
     1021 ttggattctt tgcgatagtt cacaatatcc agcatttcct ggcttaaggt gaacatttta
     1081 tcggcatagc gatagaccag ttctcccagc tcgctgggtt cgagaccacg tcccttgcgt
     1141 ttaaataatt tgccttgcag gcgctcttcc agcgctcgaa tctgtccggt aatggtttgt
     1201 ggagttaaat aaagcgcctc cgctgcgcca accacggaac cttctttata gacatgccag
     1261 aagtaataca agtggttgta attgatatga gacatgctca tttctctccc tgataacaat
     1321 gaaaagggag ccgtttatgg ctccccggta aaccgtcctg tcaaactgat ggacgcaaac
     1381 gaacgcgtaa ccagctgtat ccaattaccg ccgaagagat agaaccgacc aggataccga
     1441 gtttcgccca gttaatcagt tctggatcta cgctaccaaa ggccaggctg gcaataaaga
     1501 tagacatagt aaaaccgata ccgcacagga tccccaccac cataatttgc tgataagtcg
     1561 ttccctcagg cagatgcgcc agtttcaaac gcagcgccaa ccagcagaac agactaatcc
     1621 ccagcggttt gccaatcagc aagccagcga tgatccccaa tggcagaatg gaggtcaagc
     1681 catccagcgt gacgccttgc agtgaaacgc cagcattagc aaatgcaaac agcggcaaaa
     1741 tcagatacgc cacccacggg tgcaacacat gctccagtcg cttcgctgga gaacgcccat
     1801 gcttctcttt caaaggaata aagaagccga caattacccc cgccagagtt gcgtgaaccc
     1861 ccgatttcaa caccgcagtc cacaacacca cgccaacaag aatatagacg cccgtgcggc
     1921 gtacaccaca cagattcaat accgcgagta ccgcaattgc tacagccgcg acgccaagag
     1981 aggccatcga taagtcatta gtgtagaaca atgcgatgat aatgatggcc ccaagatcgt
     2041 cgataatagc cagagccatc aaaaagatct tcagcactaa cggaacacga cttcccaaca
     2101 gcgccagtac accaagtgca aaagcaatgt cagtagccgc cgggatcgcc cacccttcgc
     2161 gggtaatcgg atcggcatag ttaaaagcca gatagagtaa tgccggcaca atcatcccac
     2221 caatagcggc gataactgga aatgcggcct ggcgtaagct ggctagcgat ccttgcatca
     2281 gttcacgttt aacttccaga ccgactaaca ggaaaaatac cgccatcagc gcgtcattta
     2341 tccataacag catgtttttg ttgatttcga gtgaaccaac ccggagctga accggcgtct
     2401 ccagaaagtc gtgataccat ccactggttg cgccgctgtt ggccataatc atcgccagga
     2461 tagcggcaat gataagaata atgcctcccg aggcatcact gctaaagaat cgatgcagat
     2521 gtttcacttt ttatttctct ttcaggtgaa tagatcgttt ttactatttt accccgcacg
     2581 aatcatcgtt aaaatcagat tatagtgtag gaataattcg ttttttacga gccattgaag
     2641 aatttgcgcc atcagcggtg agatgcgttt ttgacggctg taagcgctca cgagtgaccc
     2701 taagcccaga taatcatagc ttaaggtgag gattcctgag ggattaatta ggaaaagtaa
     2761 taattaattt gttgtcagtc ggcgatgctt gttaggccaa tatttcaaaa ataatccagc
     2821 ttgtcgtcaa taattgcgat gaagagataa attaaagtta aataattaaa tcattaaaaa
     2881 attacgccac aaaaatgcga ggcgtcttca ggattagaat cgataaaaca aaatgggtta
     2941 aagagagcga tataatagcg gcgggtgctt gaggctgtct gtctcaggca ttagctgaac
     3001 ggcagataga gaaaagcccc gagtgatatt ttaccatcaa cccgaggcct cctatatgct
     3061 gaacacatgt agagtgcctc ttactgaccg taaggtcaag gagaagagag caatgaagca
     3121 gcataaggcg atgattgtcg ccctgatcgt catctgtatc accgccgtag tggcggcgct
     3181 ggtaacgaga aaagacctct gtgaggttca catccgaact ggccagacgg aggttgctgt
     3241 tttcacggct tacgaatccg agtaagagca acggcgggga gtgatccccg ccactttaca
     3301 ggtgctcgca tatcttcaac gcaccctatt tttacccagg cctgcccacg ggcaggcttt
     3361 tggggaggtt agcgggtcag gtcgtcaaaa aacttcttca caccatcaaa gaagctcttt
     3421 gagcgcgggc tgttgtgctc gccggttggg ccaccgaagc tttcttgcag ctcttgcagc
     3481 agctgtttct gcttttcgtt caggcctacc ggtgtttcga cgacaacgcg gcacagcaaa
     3541 tcaccctgtg cgccaccgcg gacagacttg acgcctttac cgcgcatacg gaatagctta
     3601 ccggtctggg tttcgccagg cactttcagt ttgacgcgac catcaagggt cggtacttcg
     3661 atttcgccac ccagcgccgc catagcgaag ttgatcggga cttcgcaata caggttgttg
     3721 ccttcacgct cgaaaatcgg gtgctgttta acctgaacct gaacgtacag atcgcctgcc
     3781 ggtgcgccat gctcgcccgc ttcaccttcg cccgcaagac ggatgcggtc tccagtgtcc
     3841 acccctgccg ggattttaac ggacagcgtt ttgctgcgct caacacgacc atgaccatga
     3901 catttgttgc acggatcttt gatcagcgta ccgcggccct gacagtgtgg acaggtctgc
     3961 tgtacagcga agaatccctg gcgcatctgc acctgaccag aaccatgaca ggtcggacag
     4021 gtctgcggct gtgtacctgg ttttgcaccg ctaccgtggc aaacgtcaca ctcttccaga
     4081 gtcggaatgc ggatctcttt ggtcacgcca cgtacagctt cttcgagggt gagctccatg
     4141 ttatagcgta aatcagcacc gcgcgccgca cgttgacgac cacgtccgcc gccaaaaata
     4201 tcgccgaaaa cgtcaccaaa aatatcgctg aagtctgcgc cgccgccaaa accaccgccg
     4261 cccatgcctc cttgctcaaa cgcagcatga ccatactgat cgtatgccgc acgtttttgc
     4321 gagtcggtca gaacttcata agcttccttg atctctttaa atttcgcctc ggcctctttg
     4381 tcaccctggt tacggtccgg gtggtatttc atggccaggc gtttgtaggc ctttctgatt
     4441 tcacgctctt ccgctgtttt ggaaacgcct aaaatctcgt aataatcttg cttagccatc
     4501 ttttttaaat tgcccctaga tgaatgcacg ggcggagagg aaattcccct tcgcccgtgt
     4561 cagtataatt acccgtttat agggcgatta ttttttgtct ttgacttctt caaattcagc
     4621 gtcgacaaca tcgtcatctt tcgcgttgtt tgcagaagca tcagcaccgg cagtctgctg
     4681 ctgggcatgt tgctgctggg cgatttccat cagtttctgg gaaacctgtg ccagttcctg
     4741 cattttcgct tcgatagcgg ctttgtcttc acctttcaga gcagtttcca gtgcagtcag
     4801 cgcagactcg atagcagttt tgtcgtcagc cggcagtttg tcgcctgctt cttcaacctg
     4861 cttacgggtg ctgtgcagca gatggtcgcc ctggttgcga gtctgtacca gctcttcaaa
     4921 cttacggtca gcttcggcgt tagcttctgc gtcgcgtacc attttctgga tttcatcttc
     4981 gttcagacca gaagaagcct tgatggtgat cttctgctct ttaccgctgt ttttatcttt
     5041 cgcggaaacg tgcaggatac cgtcagcatc gatatcgaag gtaacttcga tctgcggcat
     5101 gccgcgcggt gccgggttga taccatctag gttgaactga cccagagatt tgttatcagc
     5161 cgcacgttta cgttcaccct gcagcacatg gatggttacc gcagactggt tgtcttcagc
     5221 ggtagagaac acctggctgt gcttggtcgg gatagtggtg tttttcgcga tcagcgtcgt
     5281 catcacaccg cccatggttt cgatacccag agacagcggg gtaacgtcca gcagcagtac
     5341 gtcttttacg tcgccagtca gaacaccacc ctgaacagca gcaccgattg ctacagcttc
     5401 gtccgggtta acgtctttac gcggctcttt accaaagaac tcagcaactt tcttctgaac
     5461 cattggcata cgagtctgac caccaacgag gataacgtcg tcgatatcag atacggacag
     5521 gccagcatcc tgcagtgcaa ctttcagcgg ctcaatggaa cggtttacca gatcttcaac
     5581 caggctttcc agtttcgcac gagtcacttt gatgttcatg tgtttcggac cggtcgcgtc
     5641 tgcagtgatg tatggcaggt taacgtcggt ctgctgagcg gaagacagtt cgattttcgc
     5701 tttttctgcc gcttctttca ggcgctgcat tgccagcgga tcgttgcgca ggtcaatgcc
     5761 ctgatctttc ttgaattctt caaccagata gttgatcaga cggctgtcga agtcttcacc
     5821 acccaggtgg gtatcaccgt tggttgccag aacttcgaag gttttttcgc cgtcaacttc
     5881 gtcgatttcg ataatagaaa tatcgaaagt accacccccc aggtcataaa ccgcgatagt
     5941 acggttgcca gtgcctttgt ccagaccgta agccagcgca gctgcggtcg gttcgttgat
     6001 gatacgtttt acttccagac cagcgatacg gcctgcgtct ttggttgcct gacgctgagc
     6061 atcgttaaag tatgccggta cggtgataac agcttcagtt accggttcac ccaggtaatc
     6121 ttcagcggtt ttcttcattt ttttcagcac ttcagcagaa atctgcggcg gtgccatttt
     6181 ctggccttta acttcgaccc atgcgtcgcc gttatcagca gcaataattt tgaacggcat
     6241 gatggaaaca tcacgctgta cttcttcgtc ctggaagcgg cggccaatca ggcgtttaat
     6301 cgcaaacagg gtgttttgcg ggttcgtcac tgcctgacgt ttagccggct gaccaaccag
     6361 agtttcacca tcctgggtat aggcaatgat agaaggcgtg gtgcgatcgc cttcggcgtt
     6421 ctccagcacg cgaggaatgg tgccatccat aatcgctaca caagagttgg tagtacccag
     6481 gtcgatacca attattttac ccatctaaac gtctccacta tatattcggt catcatgtgg
     6541 ttgtgagtct gtaatagggg cgaaacgtct ggtttcaact gcccaatttc atttttttgc
     6601 agactcactc actgcggttg actactaaat ggggtcgtaa cccacgtcat caagggggag
     6661 atgcaaaaat tttttgtgct tttgcgcaga aatttcgcga tatggcagga caagacgtaa
     6721 aattgacagc aattttattc atataaagaa tgaatcgcag ccaaggcgtt catcaaaaaa
     6781 ttgtaataaa aagaaaagat tacgtgcctg aatcttctct ttatcagcag taaactagtg
     6841 ggtattcatc cccctacctc ttcccactaa gagaatcctt atgaaatccg tttttacgat
     6901 ttccgccagc ctggcgatta gcctgatgct gtgctgcacg gcgcaggcaa acgaccataa
     6961 actcctcggc gtcattgcaa tgccgcgtaa cgaaaccaac gatctggcgc tgaaacttcc
     7021 tgtttgtcgc attgtgaaac gcatacaact ctctgccgac catggcgatt tacagttaag
     7081 tggtgcatcg gtttatttca aagccgcccg tagcgccagt cagagcctga atattccttc
     7141 agaaataaaa gaagggcaaa ccactgactg gatcaacatt aacagcgata atgacaataa
     7201 acgctgcgtc tcaaaaatca ccttttccgg tcatacggtg aactcatcgg atatggccac
     7261 gctgaaaatt atcggcgacg actaacgcca gaatatagaa gccacaaaaa atgaatgtta
     7321 attacctgaa tgattcagat ctggattttc ttcagcattg tagtgaggaa cagttggcaa
     7381 atttcgcccg attgctcacc cataatgaaa aaggcaaaac tcgcctctcc agcatactga
     7441 tgcgcaacga actgtttaaa tcgatggaag ggcatcccga gcaacatcgc cgcaactggc
     7501 agctgattgc cggagaatta cagcattttg gtggcgatag tatcgccaac aaactgcgcg
     7561 gacacggtaa attgtatcgg gccattttgc tcgatgtttc aaagcgattg aagctgaaag
     7621 ccgacaaaga gatgtctacg tttgaaattg agcaacagtt actggaacaa tttctgcgta
     7681 atacctggaa gaaaatggac gaggaacata agcaggagtt tctgcacgcg gtcgatgcca
     7741 gggtgaatga gctggaagag ctgctgccgc tgctgatgaa agacaaatta ttggcaaaag
     7801 gtgtgtcgca tttgctttcc agccaactga cccgcatttt acgcacccac gcagcaatga
     7861 gcgtacttgg gcatggtttg ctgcgcggcg cggggctggg aggccctgtg ggcgcagcat
     7921 taaatggggt taaagcggtc agcggcagca cctatcgcgt gacgattcca gccgtactgc
     7981 aaatcgcctg cctgcgccgg atggttagcg ccactcaggt ctgataccgg aaaccgggga
     8041 aaattttcat tttcccctca aaagatcgta gacactgccc cactggctga ttattatgcc
     8101 gcgccctgaa aacactacag ttattcaggg aaattatttc accattcatt cgatgatgat
     8161 ttttgaggaa ttatgggcaa cactaagttg gctaatccgg caccgctggg cctgatgggc
     8221 ttcggcatga ccaccattct gcttaacctg cacaacgtgg gttatttcgc tctggacggt
     8281 attattcttg ccatgggcat tttctacggc ggcatcgcgc aaatttttgc tggtctgctg
     8341 gagtacaaaa aaggcaacac tttcggttta accgcattca cctcttacgg ttctttctgg
     8401 ctgacgctgg ttgcgattct gctgatgccg aaactgggtc tgaccgatgc gccaaatgca
     8461 cagttccttg gtgtctacct gggtctgtgg ggcgtattta cgctgtttat gttcttcggc
     8521 actctgaaag gcgcgcgcgt cctgcaattc gttttcttta gcctgaccgt gctgtttgcc
     8581 ctgctggcga tcggtaacat tgccggtaat gccgcgatca tccactttgc cggctggatt
     8641 ggtctgatct gcggtgccag cgcaatctat ctggcgatgg gtgaagtgct gaacgagcag
     8701 tttggtcgca ccgttctgcc gattggtgaa tcccactaat tgtcttgaga tccccccgct
     8761 cggggggatt tttttattcg ctaacgtcgc gtcttgcact cttcggtctg aatgcagcaa
     8821 ccacttctgg tgccgtttca acgtatggcc cttccagcaa ctgaatgcag tacggtacgc
     8881 tggcaaaaat accatgcacc acaacgttac cctcagcgtc cttcacacct tccagcgtct
     8941 ctttaataga cttcggctga ccgggtaagt taaggatcag cgcctgtttg cgaatcacgc
     9001 ccacctgacg cgaaaggatc gcagttggta caaaatgcag gctgatctgg cgcatctgtt
     9061 caccaaagcc aggcatctcg cggtccgcta ctgccagcgt cgcatcgggc gttacgtcac
     9121 gacgcgccgg gccagttccg cccgtggtga gcaccagatg gcaactcatt tcatccacca
     9181 gctcacacaa cgtttgctcg atgatcgcct gctcatcggg gattaagcgg gtttccagtt
     9241 caaacggcgt ggttagcgcc gatgtcagcc attcttccag cgctggaatg cctttatcct
     9301 gataaacgcc gctggatgcg cgatcagaga tggaaactaa gccaatacgt aaagtattca
     9361 tcaagatttt tccgtttaaa caatctcgtt aaacagaatg atacactgcg aagggagtga
     9421 cagacaggct tcagaagagg tagcgtgacc gacttcccgg tcacgctaag aatgattaca
     9481 gcagatcgcc gatcattttt tccagttttt cctggtcaat agcaaactta cggatacctt
     9541 ccgccagttt atctactgcc attggatcct ggttgtgctg ccacaggaac tcggactcag
     9601 tgatacgcgc cggacgcgct ttcacttcgc cggtgtaaga cagtttacgt tcgatagccc
     9661 cttcgctctc cgccagctct ttcagcagtg ccggtgcgat ggtcagacgg tcacagcctg
     9721 ccagttccag aatttcgccg atgttacgga agcttgcgcc cataaccacg gtttcataac
     9781 cgtgctcttt gtagtactgg tagatttcag atacagaaac cacgcccgga tcttctgccg
     9841 gagcgtactc tttcttatcg gtattcgctt tgtaccagtc aagaatacgg ccaacaaacg
     9901 gcgagatcag gaacacgccc gcctccgcac aagcacgagc ctgagcgaag gagaacagca
     9961 gggtcaggtt acagttgata ccttcttttt ccagctgttc tgcagcacgg ataccctgcc
    10021 aggtagaagc cagtttgatc agaatacgat cgttgctaat acctgcatcg ttgtagagtt
    10081 tgatcaggcg ttttgctttc gcaattgacg cttcggtgtc ataggaaaga cgcgcatcaa
    10141 cttcagttga gatacggccc ggaaccagtt tcaggatttc cagaccaata tttactgcca
    10201 gtttgtcggt cgcgtccacg atctgctgcg cgcgatcgtt gctctgctgt ttcgcccagg
    10261 cgacagcatc atcaatcaac ttacggtatt ccggaatctg cgctgcgtta agaatgagag
    10321 aagggttggt tgtggcatcc tgcggttgat acagcttcat tgccgcgatg tccccagtgt
    10381 cggccactac ggtggtgtac tgacgaaggg aggtcaattt gtccgtcatg atagtatttc
    10441 tctttaaaca gcttgttagg gggatgtaac cggtctgccc tgatgatatc acgacgcaac
    10501 gtcagcgcaa ccgccgacaa gacggttatc gccaggcgag actgtttcgg atttctgaca
    10561 ctgcgttgat atcgcccacc atttttatac aaaacctcat gtatgctacg cagaagttat
    10621 caagtacctc gtagcgtata tacttcttaa acaattggta acgtttacac aggaaagtca
    10681 tcgcgaccgg caataagagg gatatgcatg ccagattttt tctcctttat taacagcgtc
    10741 ctttggggat cggtaatgat ttacctgctc ttcggcgcag gttgttggtt cacttttcgc
    10801 accggatttg tgcagtttcg ctacatccgc cagtttggca aaagtcttaa aaatagcatt
    10861 catccacagc caggcggttt aacctcattt cagtcattgt gtaccagtct tgcggcgcgc
    10921 gtgggtagcg gcaacctggc cggcgttgcg ctggcaatta ccgccggtgg accaggtgcc
    10981 gtcttttgga tgtgggttgc tgcgtttatc ggcatggcga cctcgtttgc cgaatgttcc
    11041 cttgcacaac tttataaaga acgtgacgtc aatgggcagt ttcgtggcgg accggcatgg
    11101 tatatggcgc gcgggctggg gatgcgctgg atgggcgttc tgttcgccgt ctttttgctc
    11161 atcgcctacg gcataatttt cagcggagtt caggcgaacg ccgttgcccg agccctgagt
    11221 ttttcttttg attttcctcc gctggtgacg ggcattattc tcgctgtctt tgctctgctg
    11281 gcaatcactc gcggtcttca tggcgtcgcc cggctcatgc aggggtttgt cccgttgatg
    11341 gcgataatct gggtactgac cagcctggtg atttgcgtaa taaatatcgg gcaacttccc
    11401 cacgtcattt ggtctatttt tgagagtgct tttggctggc aggaagcggc aggcggcgcg
    11461 gcgggatata ccttaagcca ggcgattact aacggttttc agcgcagtat gttttccaat
    11521 gaggtgggaa tgggatcgac gccaaacgcg gcagcggcag ctgcatcctg gcctccgcat
    11581 ccggcagcac aagggattgt ccagatgatt ggcattttta tcgacaccct ggtcatctgt
    11641 acggcaagcg ccatgctgat attactggcg ggtaacggca caacctacat gccgctggaa
    11701 ggtattcagc ttatccagaa ggcgatgcgg gtgctaatgg gttcctaggg tgctgaattt
    11761 gttaccctcg tggttattct gtttgccttc agctccatcg ttgccaacta catttatgcc
    11821 gaaaacaatc tcttcttttt acgcctgaac aaccctaaag cgatctggtg tttgcggatc
    11881 tgcaccttcg caacggtcat cggcggcacc ttgctaagcc ttccgctgat gtggcaactg
    11941 gcagatatca taatggcctg catggctatt accaatttga ccgccatttt actgctctcg
    12001 cctgtggttc ataccattgc cagtgattat ctacgccagc gtaaactcgg cgtgcgcccg
    12061 gtgtttgatc cgttgcgtta tccggatatc ggtcgccagc tttctccgga cgcgtgggat
    12121 gatgtttcgc aggagtaatc acaactatcg atcaactcat tctctttttt tgctaaagtc
    12181 ggcataaatt tcctgcaagg actggatatg ctgattctta tttcacctgc gaaaacgctt
    12241 gattaccaaa gcccgttgac caccacgcgc tatacgctgc cggagctgtt agacaattcc
    12301 cagcagttga tccatgaagc gcggaaactg acgcctccgc agattagcac gctgatgcgc
    12361 atcagcgaca aactggcggg tatcaacgcc gctcgctttc atgactggca gccagatttc
    12421 acgccggcga atgcccgcca ggcgattctg gcgtttaaag gtgatgtcta caccggcttg
    12481 caggccgaaa ccttcagcga agacgatttc gattttgccc aacagcattt gcgaatgctt
    12541 tccggcttgt atggcgtact gcgcccgctc gatttaatgc agccttatcg tctggaaatg
    12601 gggatccgtc ttgagaatgc ccgagggaaa gatctgtatc aattctgggg agatatcatc
    12661 accaacaagc tgaacgaggc gctcgcagca caaggcgata atgtggtgat taatctggcg
    12721 tcagatgagt attttaaatc ggtgaagccg aagaaattga atgccgagat tatcaagccg
    12781 gtgttcctcg atgagaagaa cggcaaattt aagatcatca gcttctacgc taagaaagca
    12841 cgcggtctga tgagtcgttt cattattgaa aatcggctga ccaaaccaga gcaactgact
    12901 ggttttaata gcgaaggtta cttctttgat gaagattcct ccagcaatgg cgaactggtg
    12961 tttaaacgct acgagcagcg ttaagtttgt agtcaatgcc ggatgcggcg tgaacgcctt
    13021 atccggccta caaaagcatg cagattcaat atattgcaga attaacgtag gcctgataag
    13081 acgcgccagc gtcgcatcag gcgctgaatg ccggattgtt acccggcatt tgtcatttag
    13141 cggtgatgtt tgcctggacc atgaccgccg tgatgatcat gaggagcttt cttatggtgg
    13201 cgcggcggtg gcggcggtcc gtgtgggtgc cagcgattgc ctcgccattc ataatgttgt
    13261 ttccaccagc cgtggtcgcg ccagtgacct ccatcccagt aatagccacg attatcacga
    13321 tcgcctatct gtaattttac tgacgggact aacgtaattt ccgcagcctg tgctgccatg
    13381 ggagcgacca gaaccaggga aagtgcgagt acgatagatt gcatcttttt cacaggttac
    13441 tccttttatc attgcccgtt atgggtcgat atccggagat tacgcgatga gaacgacggt
    13501 tgttattctc cgcaatccta atctctaagt taccgcattt attgataatt tctccttttt
    13561 ccctgtcgtt tttctccata atttcttcat aaaaaagccg ggctgcataa aagcaaaccc
    13621 ggcctgattg agataatgaa tagattttac tgatgattca tcatcaattt acgcaacgca
    13681 gaaaaatcgg cgggcagatt atgtgaaagc aagggtaaat cagcacgttc tgccagctct
    13741 tttggcagat ccaacgtttc accgagaatc gcttccacgc tctctttaaa tttcgccgga
    13801 tgcgcggtgc cgaggaacaa gccatattcg cctggattca actggtcacg cagcgcacga
    13861 tacgctacgg cagcgtgcgg ctccgaggtg tagcccagtt cttttaactc acgcattgtc
    13921 tgttgcgtgg tttcatcatc cacggctgca taacccagct ctttcagttg ccagattttg
    13981 cggcggaaca actcttccac acgcggccag ttgttcggct gactcacgtc catcgcgttg
    14041 gataacgtcg cctgagtcgc tttgggtgac cactgaccgt cgtgcaggaa acgtggcacg
    14101 gtatcgttca cgttggtcgc agcaataaaa cgtttcaccg gcagaccgaa tgacttcgcc
    14161 agcagacccg ccgtcaaatc gccgaagttt ccgcttggca ccgagacaac cagctggttg
    14221 cgcgcctcct gcggcagctg cgcaacagct tcaaagtagt agcaaatctg cgccagcaaa
    14281 cggctgatgt taatcgagtt agccgagttt aaccccagcg ccactttcag ttcttcatca
    14341 tcaaacgcct gcttcaccag cgcctgacag gcatcgaaat cgccgtcgat ggcaacagtt
    14401 tcgatattgc cgcccaacgt acagaacagt ttttcttgca gtggactgat tttgcctcgt
    14461 ggatagagga taaccacttt cacattcggc aaaccgtaga aagcatgagc cactgcagct
    14521 ccggtatcac cggaggtcgc ggtcagaatg gtcactggct tatcatccgc aatatgggtc
    14581 agcatttgtg ccataaagcg accgccgaaa tctttaaatg ccagcgttgg cccgtggaac
    14641 aattccagac aaccgacatc gctttcaaca ttggcgaccg gagccgggaa ggcaaacgcc
    14701 gcgcgcacgc gctcttccag gatttcctgt gggatttcat caccaataaa cgccgagagg
    14761 atcttcgcac tgcgggtgac aaaatccagc ttcagcatct catcaatttc agtcaggctg
    14821 aattccggca ggtcgtgcgg aaaaaacagc ccctgatttt tgcccaaccc ctgggttacg
    14881 gcttgcgcaa agctgacctg ctcgttgtga tctttcagat tgtagagttt catttagttt
    14941 tccagtactc gtgcgcccgc cgtatccagc cggcaaatat gaacaaaacc ttcctgattt
    15001 tgcaggtagt tcttacccaa ccagtcggca acgcgctggg cggtatccgg cttgtcacat
    15061 agagcgaaca aggtcgggcc ggagccggag ataccgctca ctaccgcgcc gatttccgcg
    15121 accgcctgcc gcgcctgccg gaagccaggc agtaaccgtt cacggtaggg ttcagcgata
    15181 acatctttca tcagcttcgc ggcaagctca ggctgacggg aatagcaggc gtgaatgaag
    15241 ccagccagat gtcgcccgtg cgcaatgcaa tcctggcggc gatactgcgc cggtaaaata
    15301 gcccgggctt ctgccgtcga gactttaatt cccggatacg ccagcaccca cagccactca
    15361 tcaaaccctg gcacttgctg gctgatgatg tcgttttctt cgatcatcaa ctgcatacca
    15421 ccaagaaaac acggtgccac gttgtcgtaa tgaatgctgc cggagatacg tccttccagc
    15481 tcgcccatca aagccagcaa acgagtgtca ttaagcggct tgccgcagtg ttcattcatc
    15541 gccatcagcg ccgcgaccac cgaacaggcg ctggagccta agcccgaacc gatcggcata
    15601 ttcttttcca gagtcatcgc cactggaatt tgcttgccaa gctcctggca aaaacgctcc
    15661 cagcactgat aaacgatatt ttcccgtggt tctgacggca gcttatcggc aaagcgtccg
    15721 aggttgttga gactgaatgt ctctgccgcc tcaaccgtga ctacatctcc gagcaatgca
    15781 ccatcaacgg gtgccaccgc cgccccgagc acatcaaacc cgacgctcat attggcactg
    15841 gaagccgggg cataaacttt aaccatgtca gactcctaac ttccatgaga gggtacgtag
    15901 cagatcggca aagacaccgg cagcggtaac gtcattgccc gcaccatatc cgcgcagcac
    15961 caacggcagc ggctgataat agtggctata aaaggccagg gcgttttcgc catttttcac
    16021 tttgaacagc ggatcattcc catccacttc ggcaatcttc acgcggcaga caccatcttc
    16081 atcaatattg ccaacatagc gcaaaacttt tccttcatca cgggccttcg ccacgcgcgc
    16141 ggcaaagaga tcgtcgagct gtgacagatt cgccataaaa gcggcaacat caccctcggc
    16201 gttaaactct gcgggcagca caggttcaat ttcaatatcc gccagctcca gttcacgtcc
    16261 cgtttcacga gcgagaatca atagtttacg cgccacatcc ataccagaaa gatcatctcg
    16321 cgggtccggt tcggtataac ccatttcccg cgccagcgtg gtcgcctcgg agaaactcat
    16381 gccttcgtct aacttgccga agatataaga aagcgaacct gaaagaatgc cggagaactt
    16441 caccaattca tcacctgcat tgagcagatt ttgcaggttc tcaataaccg gtaatccagc
    16501 cccaacgttg gtgtcataga ggaatttacg ccgcgatttt tccgccgcat aacgcaactg
    16561 atgatagtaa tccatcgacg aggtgttggc ctttttgttc ggcgtgacaa cgtggaaacc
    16621 ttcgcgcagg aagtcggcat attgatccgc cactgcctgg ctggaggtgc agtcaacaat
    16681 gaccgggttc agcagatgat attctttcac gaggcgaatt aagcgcccga gattaaacgg
    16741 ctctttggct tgtgcaagtt cttcctgcca gttttccaga ttaaggccat gcacactagt
    16801 gagcagagcc ttcgagttgg caacgccgca gacacgtaag tcgatatgtt tattcttcag
    16861 ccagctttgc tgacgcttca gttgctccag cagcgcaccg ccaacgccac cgacgccaat
    16921 cacaaacact tcgataacct gatcggtatt gaacagcatc tgatgagtaa cgcgcacgcc
    16981 agtggtcgca tcatcgttat ttaccacgac agagattgag cgttcagaag atccctgagc
    17041 aatggcgaca atgttgatat tggcgcgggc cagcgcggca aagaatttcg ccgagatccc
    17101 acgcaaggtg cgcataccat cacctaccac cgagataatg gccagccgtt ccgtcaccgc
    17161 cagcggctcc agtaagcctt ctttcagttc caggtagaac tcttcctgca ttgcccgttc
    17221 agctcgcaca cagtcgcttt gtggaacgca gaaactgata ctgtattcgg aagatgattg
    17281 cgtaatcagc accacggaaa tacgggcgcg tgacattgca gcaaagacgc gcgccgccat
    17341 gccaaccatc cctttcatcc ccgggccgga aacgctgaac attgccatgt tattcagatt
    17401 ggaaatgccc ttgaccggta attcgtcttc atcacggctg gcaccaatga gcgtacctgg
    17461 tgcttgagga tttccggtat ttttaatcag gcaagggatc tggaactggg cgatgggggt
    17521 aatggtgcgg gggtgaagaa ctttagcgcc gaagtaggaa agctccatcg cttcctggta
    17581 ggacatcgac ttcaacaacc tcgcatcggg cacctgacgc gggtcgcagg tatagacccc
    17641 gtcaacgtcc gtccaaatct cgcaacaatc ggcgcgtaaa caggcagcca gcaccgccgc
    17701 ggagtagtcg gaaccgttac gtccaagcac caccagttcg cctttttcat taccggcggt
    17761 gaaacctgcc atcaacacca tgtgatcagc cggaatgcgg cttgccgcaa tacggcgggt
    17821 ggactcagca atatcgacgg tagattcgag gtaatgcccc actgccagca gtttttcgac
    17881 cggatcgata acggtaacgt tgtgaccacg cgcttctaac acgccggcca taatggcgat
    17941 cgacattttc tcgccacggc aaatcagcgc agcgttgatg ctatccgggc actgccccaa
    18001 caaactaatg ccatgcagga catgttttat ttgggcaaat tcctgatcga cgaaagtttt
    18061 caattgcgcc agcgggaacc ccggctgggc ggcggcgagt cccgtcaaaa gttcggcaaa
    18121 aatacgttcg gcatcgctga tattgggtaa agcatcctgg ccgctaatgg ttttttcaat
    18181 catcgccacc aggtggttgg tgattttggc gggggcagag aggacggtgg ccacctgccc
    18241 ctgcctggca ttgctttcca gaatatcggc aacacgcaga aaacgttctg catttgccac
    18301 tgatgtaccg ccgaacttca acactcgcat ggttgttacc tcgttacctt tggtcgaaaa
    18361 aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt cctgtacgcg tcagcccgca
    18421 ccgttacctg tggtaatggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc
    18481 atggatgttg tgtactctgt aatttttatc tgtctgtgcg ctatgcctat attggttaaa
    18541 gtatttagtg acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga
    18601 agctgctatc agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag
    18661 aatgaaaagc tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata
    18721 tcatgcagca aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgc
    18781 tgtaaccagt cgaccagttt tatgtcatct gccactgcca gagtcgtcag caatgccatg
    18841 actcgttcgc gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgccggt
    18901 tgttgtatta atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc
    18961 gaaggataat ccgccaccat cggcacacca gtaagcacgt cagccaacgc taactcttcg
    19021 ttagtcagcc cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat
    19081 ttttcctcta acagcggcac cagttctact ggcgtggcgt agtaatgata tttcgcccga
    19141 ctacgcgcag tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg
    19201 aaaactttaa tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc
    19261 tccaggtgtg cctgactatc gacaatccgc agttcgctaa aacccatcgt tttcattgcc
    19321 cgcgccgctg ccccaatatt ttctgctctg gcgggtgcga ccaggataat cgttatacgc
    19381 atattgccac tcttcttgac caaataaccg cgaacagggt gatcactgtc aacttattac
    19441 gcggtgcgaa tttacaaatt cttaacgtaa gccgcagaaa aagcccttta cttagcttaa
    19501 aaaaggctaa actattttct gactgcacta acggtttaat tgttaaaaaa tgctacatat
    19561 ccttctgttt acttaggata attttataaa aaataaatct cgacaattgg attcaccacg
    19621 tttattagtt gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat
    19681 ttagatacga tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa
    19741 cgaagcgtag ttttattggg tgtccggccc ctcttagcct gttatgttgc tgttaaaatg
    19801 gttaggatga cagccgtttt tgacactgtc gagtcctgag ggaaagtacc cacgaccaag
    19861 ctaatgatgt tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc
    19921 atgttacgcc gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact
    19981 tcctgtttcg atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat
    20041 cgttgaagac gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta
    20101 tgatgttttc gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat
    20161 caacctggtg atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga
    20221 actgcgcgag caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga
    20281 taaaattctc ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg
    20341 tgaactgacg attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag
    20401 cgaagaacgt cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag
    20461 ccgttcgttg atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc
    20521 catgcttcac ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac tgctgaagaa
    20581 aatgaccggc cgtgagctga aaccgcacga ccgtactgta gacgtgacga tccgccgtat
    20641 tcgtaaacat ttcgaatcta cgccggatac gccggaaatc atcgccacca ttcacggtga
    20701 aggttatcgc ttctgcggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaacg
    20761 gcgcttttta gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc
    20821 gttgtaactt ctttactggc tttcattttc ggcagtgaaa acgcatacca gtctatatta
    20881 cgagtcacaa acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc
    20941 gcgctatcgg cagagttgag cagtccccac atcactccat ccagcaacaa cagcgcgagg
    21001 gtaaacaaca tgctattgcg ccagcctttc aataccgcct gcaaataaat accgttcatt
    21061 aacgccccaa tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc
    21121 gccagcaaga gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg
    21181 tgtaaacgtt gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc
    21241 agcagaatgg cgtacttagt cgcccggtcc gttaattggt attgatcggc tggcgtcgtc
    21301 actgcgacgc taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc
    21361 tcaccgagat tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta
    21421 gcttcccgtt tggctggtag aaaatcacct aaaaaactgg gatgcggcca gttgctggtt
    21481 aaggtcattt cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa
    21541 ttcagtgcca tattcagctg caggttctgc ttacgccagt ccccttcggg taaagggata
    21601 tgcacgccct gcccgccttg ctctaacccg gtaccgggtt caatggtcag tgccgttccg
    21661 ttaacttcag gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata
    21721 aagggcttgc ctaagatgat atttggcgcg ttgagctcgc taagacgcga aacatcgaaa
    21781 tcggctttta gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt
    21841 tcttccacgt tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa
    21901 cttcgtttcc gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga
    21961 atagcgataa gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct
    22021 tccacatcgc tacggtaatc ggcacgttcg acaatcactt gccgaatcag cattatcgga
    22081 atcaacaaca gcaatactgc accaaacaag gtggtcattt tccagaacag gggggatttc
    22141 aacatagtct tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga
    22201 agctatgtga agtgacggtg aagtcgtagc gaggccagca cgccaccttc ctgcacgttg
    22261 cgcagcgtga cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga
    22321 ccgctgcttt tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac
    22381 agcgcgtagt caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga
    22441 tccacttcgg cgcttagcgt tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc
    22501 agtaaattcc ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg
    22561 ggcataacat gcaaagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg
    22621 cggcgaaata atgccgccac atcaacagca gtcagaacga cttcctgacg attctccagt
    22681 cttgcctggc gtagtaacgt ttccaccagc gcctgcattc gcgcattttg tgtcagaatg
    22741 ttgtcagtaa aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg
    22801 ccacgaatcg ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca
    22861 atatagtttt tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc
    22921 agctcgctac taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca
    22981 tagcgagtga gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc
    23041 agtgcaatcc ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg
    23101 accggagcca tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg
    23161 cccataatcg gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac
    23221 gtgctgcgcg caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa
    23281 tcctgtccaa cggctttatt tgccgaatcg aacaatactt tgccatgcgc atcggtcata
    23341 tagacatgat attcattgcg cactttgtta atgccaccaa tattggcgcg aaacgggcga
    23401 tgttgtagct gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa
    23461 tcgggacgcg ccagctccgc aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct
    23521 cttcgcacgc ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc
    23581 gccaccagta aaaaatagcc cagcagcaac cgcatgccga tacgcattac aagcccctca
    23641 ggctatatcc catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca
    23701 gcttggcacg cagcgtttta atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat
    23761 cttcccacac gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta
    23821 acgtcttcag caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga
    23881 tctgcgccgc gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgtcg
    23941 agaacttctt cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa
    24001 acggtttagc cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt
    24061 cacttcgggc cgtcaggaac agtacaggta acgccggatg gagcgccagt aactggcggc
    24121 acaattcaaa gccactgata tcaggcaaac caacgtcgag aatcatgacg tcgggaacct
    24181 gctggcgagc tttatccagc accggcaaac ctcactcaaa gacctcgacg gcaaaacctt
    24241 cctgctgcaa catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga
    24301 ccgtttcccg ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc
    24361 taatcgcgtt tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg
    24421 cgttgcgttt ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc
    24481 gttttttgaa tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa
    24541 tcggcccgac ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc
    24601 cccctttaat accaccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg
    24661 gatcgtcaaa ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt
    24721 cgaccgaacc aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca
    24781 ggatcaaatg cttgtatttc atattgttac catttttatc tattacacga gcaattagtt
    24841 cgtcacggta aataacatat tgattaagaa caaaatctca attcttttat ttccgtcaag
    24901 ccctaaaaca tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat
    24961 tatccaatta cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca
    25021 ggccggcatt attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc
    25081 gctcgacaat gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa
    25141 agatgtcact ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc
    25201 ggtcgcacta cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga
    25261 ctctcaacag acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta
    25321 ccgccgttct cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt
    25381 cacaatgcca gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca
    25441 gagctactcc tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt
    25501 ctggcacgat tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga
    25561 aacgcgtccg agtcaggata aagacgacga acaagaggta ttctatacca ccgcgttagc
    25621 ccaggatcag gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga
    25681 atatgtgatg tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt
    25741 atacggaacg tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg atattgagcg
    25801 atactacccg gcagaagacg ccaaagcggg agatcgccca attaatctgc gctgtgaact
    25861 gctgattcct atccgccgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga
    25921 gatgtcccct gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc
    25981 cacgcgcgaa atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc
    26041 gagaatcgta ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct
    26101 tccctgcggt aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg
    26161 cattgactcg ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca
    26221 gttctcttct tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa
    26281 ttcacgcagg cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc
    26341 cgtacgccgg gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc
    26401 acgggttgcc acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg
    26461 gccctgaata cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg
    26521 taacatgctt tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg
    26581 caccaagttg tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt
    26641 cacgagatct tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta
    26701 ccggaacaac cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat
    26761 gcccgccgtt tacttccaga ggctgatttt tgggtggcga ttgaagctgg cattgatggc
    26821 gacagcactt tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg
    26881 gcaactttgc cgttacctgc ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt
    26941 cccgtgagtc gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg
    27001 tgtttaccgc cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc
    27061 tgagtccgtt tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg
    27121 catccgacat tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa
    27181 aatcaatcgc ttttcagcaa cacctcttcc agccactgac gcagctccac gggcgcggct
    27241 ttcaggctgt tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac
    27301 tcacgctgac tcatttcgcc acgcaacagc tcttcgacaa tacgcacgcg agtccccaac
    27361 gcttcgcgct catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg
    27421 taggcattct taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc
    27481 gctgctgaat agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa
    27541 aatgactccc ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc
    27601 agtaacgacg tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc
    27661 ggtagtaagc atcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa
    27721 tactctcgac aaatgccaca gcgtcgatac gcccggcgct gtggccaagc caggttcgca
    27781 cccgtccagg tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct
    27841 gataaacata ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt
    27901 gcccagggct gctataacca ggaatagaga acatcttcac cgtatgggtc gctgtaccag
    27961 gcataatctg catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc
    28021 tctcctgacg ggcaatcgcc atcgcatagc tttgcgggat ctctttccca ctggtgtaac
    28081 gtttgaaaag atcgttgtaa gccagcggga atcgctcttc cagatgatcc caaagcttcc
    28141 cggcgatcgt tgcctgaacg ctaagatccc accattggtt attgaaagca taccgagcca
    28201 gttgagcctg ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg
    28261 tattatcgag attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca
    28321 gggtgctgtc aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc
    28381 gttgtgctgc aaccatcggg tagaaaccac gctgttgtat gagttgatgc aaaatctctt
    28441 tcgcttcagc ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat
    28501 ctttctcttt tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc
    28561 cggtgccaag cgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa
    28621 tggcgtcatc gcgccatttc gcctgctcat cggtgacatc attgcccatc aaacgccagg
    28681 cgacgatatc gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg
    28741 acgggatcat cagccgtgca ttttcggcat cctgccgcgc cacactggca aacgccaccg
    28801 ccgccatttg acgggtaaaa tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat
    28861 tagggttgtt cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct
    28921 gccctgccag cactgttacc aggcctgtgt tacccgcttt catcgccaga cggatacgct
    28981 ctaaatacgc cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac
    29041 aggcgttagg ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt
    29101 cactctgccc ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg
    29161 gcttttccgg gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga
    29221 cgaaacgaga ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag
    29281 tgaccgtcac cgccggttga ttcatcaggt catcggtgat ctggcggtat tccaggtagg
    29341 gataaagcgg ataatccttc agtccaggca tcatttgttc gaccacatcc atctgtcgat
    29401 tatcccaggc ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc
    29461 gcgccacgct gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt
    29521 gtttggcttt ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc
    29581 gcgtaataga tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt
    29641 acttttactg cgaaggtgat cgaccaactg ggattaggcg gggaaacagg ctacacttcg
    29701 cctttgaaaa ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt
    29761 ttataccatg catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat
    29821 ctctctgagt ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa
    29881 gtccaccctg ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc
    29941 gcagccagac atcaagattg gttatctgcc gcaggaaccg cagctgaacc cggaacacac
    30001 cgtgcgtgag tccattgaag aagcggtttc agaagtggtt aacgccctga aacgcctgga
    30061 tgaagtgtat gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca
    30121 aggccgtctg gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga
    30181 gcgtgcggcg gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg
    30241 tggtgagcgt cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct
    30301 gctcgacgaa ccgaccaacc acctggatgc cgaatccgtg gcgtggctgg aacgcttcct
    30361 gcacgacttc gaaggcaccg tggtggcgat tactcacgac cgttacttcc tcgataacgt
    30421 tgcaggctgg atcctcgaac tggaccgcgg tgaaggtatt ccgtgggaag gtaactactc
    30481 ctcctggctg gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg
    30541 tcgtaagtcg atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc
    30601 gaaaggtaaa gcacgtctgg cacgctttga agagctgaac agcaccgaat accagaaacg
    30661 taacgaaacc aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga
    30721 agtcagcaac ctgcgtaaat cctacggcga tcgcctgctg attgatgacc tgagcttctc
    30781 gatcccgaaa ggggcgatcg tcgggatcat cggtccgaac ggcgcgggta aatcgaccct
    30841 gttccgtatg atctccggtc aggaacagcc ggacagcggc accatcactt tgggtgaaac
    30901 ggtgaaactg gcatcggttg atcagttccg tgactcaatg gataacagca aaaccgtttg
    30961 ggaagaagtt tccggcgggc tggatattat gaagatcggc aacaccgaga tgccaagccg
    31021 cgcctacgtt ggccgcttta actttaaagg ggttgatcag ggtaaacgtg ttggcgaact
    31081 ttccggtggt gagcgcggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat
    31141 gctgctgctc gacgaaccaa ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa
    31201 cgccctgctg gagttcccgg gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga
    31261 ccgtatcgcc acgcacatcc tggactacca ggatgaaggt aaagttgagt tcttcgaagg
    31321 taactttact gagtacgaag agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc
    31381 gaagcgtatc aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga
    31441 acgccttatc cggcctacaa aaccagcaat ttcaatacgt tgtaaaactg taggcctgat
    31501 aagacgcggt aagcgtcgca tcaggcattg agcaccgaat gccggatgcg gcgtgaacac
    31561 cttttccggt ctacaacagc cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt
    31621 tttttcaaac cttgtttaat ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa
    31681 aaactcattt tatacctcgc ctttgagcag tttcgtcacg gttatccttg ctcccccatc
    31741 atctcccgca ccagctccac gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc
    31801 cgcacgaatt cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg
    31861 cgatccaccg aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc
    31921 agcaggatca ccagatcgaa acggtattca tcaatcagcg cctgcacgaa cggatgctca
    31981 cgcccttcgt actttttgca gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc
    32041 actttattgg cgtatttcac tgcaaaatca atgtattgtg cgtggcccaa cgcgatttta
    32101 tcgtagtcgg aatactgcaa tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg
    32161 cggccatatt cccacgcact ggtggtgttg aagatattgg caagtttgtt taccagggtg
    32221 gatttaccgc tcgactcgcc gccaaggatc gccacggtac gcacaaaaaa cggcttcact
    32281 tcggtaggaa tatattccca gtagcggaac gggttttcgc ggatctgcgc accgctgata
    32341 ctcataaagg tacgtttcgg atcgaccagc accgtctcga tccccagatg ttccatatac
    32401 tgtggcgcat cggcttcttc cgaggtgtag atcaggtccg gctggatccc cttttcagcc
    32461 ataaactttt tgatgccgtt gctccacaca tcccagccat gcggatacgg ctccatgccc
    32521 tcttcgttga aagcatgaat acggatattt ttctgatatt taaaggtttg caatagccag
    32581 cgcagacgat ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg
    32641 tcgcgggtat cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc
    32701 tggataaggt agatatgtcc ggtatgcagc gggtagaact taccgaatac gacaccaatg
    32761 gttttcttct gccggggaaa ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc
    32821 gcgctggggc ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg
    32881 ctggcatcag ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa
    32941 tcaaatgacg acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg
    33001 cgtcggagag ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt
    33061 tagccgccgg aacaatcgcc cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct
    33121 ggccactggg caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat
    33181 cctgtggcag cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg
    33241 cgctggtttc ggttaccttc acgccgccga ccacgttcac aaacacatcc tgatcggcca
    33301 tttgcagacc accgtgacgg tgcaagacag ccagcaggat tgccagacgg ttttgttcca
    33361 gccccactgc cacgcggcgc ggattcgcca ttatcgagtg atcgaccagc gcctgaatct
    33421 ccaccagcaa cggacgcgtt ccttcccaca ccaccatcac ggagctgccg gaggtcactt
    33481 catctccgcg acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgttcgg
    33541 tcatcgcgaa gacgcccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg
    33601 tgcgaaaacg ggagtcggca tcgccatcca gaagcaccga acagtcgata cagtgttcca
    33661 gcactttcgg gccagccagc gaaccatctt tggttacgtg tccaaccatg acaatcgcca
    33721 caccgcgcgt tttggcgaaa cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc
    33781 taccaggcga tgattgtaca tccgccatat gcatcacctg gatcgagtca attaccatca
    33841 gcttcggttg ctcttcttcg gcaatcaggc agatctgctc gatgctggtt tccgacaaca
    33901 tattgagatt gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg
    33961 actcttcgcc ggtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca
    34021 gcaacagcgt ggatttcccc gcaccagggt taccgccaat cagaatggca cttcctggca
    34081 ccacgccgcc tcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct
    34141 cttcaaggct gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc
    34201 cgctgagacg ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg
    34261 tgttccaggc atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt
    34321 cattacaaac aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc
    34381 cgcccgcagg atgacgggca ggcaattact tctgattcag gctgccagag aggatgcaga
    34441 atacccccat caggtcagcg tgacggatgg tgacttccgc cttttcattc acttttggct
    34501 tggcatggta ggcaatcccc agccctgctg ctttgatcat cggcaggtca ttagctccat
    34561 cgccaatcgc cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgagtcagag
    34621 ttttcgcttt gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt
    34681 ccatgatctc cagttcattg gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt
    34741 cagcaaagaa agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct
    34801 tgagcaccag ctgcgttaag cctggcatca gcagcagatt ttcacgcacc tgttgcagaa
    34861 tattggcgtc agcgcctttc agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga
    34921 gttcgccgcg catcgcccgt tcggttacct ccgccaccat ctcgcccgtt ccggccagct
    34981 tggcaatttc atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca
    35041 aacccggcgt gcgcaggtgc gggattttcc ccagcggggc gacatccagc tgcgcttcgt
    35101 gggccaggcg tgtagcccgt gcggtgagtg aacctgccag acgaatcacc tgataatctt
    35161 ccacgcacca ggcggcaaca atcaccatcg ccgcacccag tttgctctgg tattgggtca
    35221 gacgctgttt atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat
    35281 ccagtggcat cacttcatca ccacttaatg aaagaggcag acccggccat aaagagacat
    35341 cttcaggcag gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag
    35401 cagggaaaat aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca
    35461 aattttcaaa gggtggaaga tggctcgcac aaaactgaaa ttccggctac atcgggcagt
    35521 gattgtcctg ttctgtcttg ccttgttagt ggcactgatg cagggggcgt catggtttag
    35581 tcaaaaccac cagcgacagc gtaatccaca gctggaagaa ctggcccgca ccctggcgcg
    35641 tcaggtgacg ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat
    35701 tcaggcgatc ctcgatcagt taacagatga aagccgtatc ctcgacgcgg gtgtgtatga
    35761 cgaacaaggc gatcttatcg cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc
    35821 gctcgacggt aaaaaagcag gcggctattt taaccagcag attgtcgagc caattgcggg
    35881 taaaaacgga ccgctcggct atctgcgcct gacactcgac acccatacgc tcgccaccga
    35941 agcccaacag gtggataaca ccactaacat tttacgcctg atgttgctgc tctcactggc
    36001 aatcggcgta gtgctgaccc gcacgctgct acagggtaaa cgcacccgct ggcagcaatc
    36061 gcccttcctg ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga
    36121 gtgacccatt actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga
    36181 ctcttacgac ccgtggttta acctggcggt ggaagagtgt atttttcgcc aaatgcccgc
    36241 cacgcagcgc gttctgtttc tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca
    36301 gaacccgtgg aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg
    36361 gcgcagtggc ggtggcggcg cggtgttcca cgatctcggc aatacctgct ttacctttat
    36421 ggctggcaag ccggagtacg ataaaactat ctccacgtcg attgtgctca atgcgctgaa
    36481 cgcgctcggc gtcagcgccg aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga
    36541 aggcgaccgc aaagtctcag gctcggccta tcgcgaaacc aaagatcgcg gcttccacca
    36601 cggcaccttg ctactcaatg ccgacctcag ccgcctggca aactatctca atccggataa
    36661 aaagaaactg gcggcgaaag gcattacttc agtgcgttcc cgcgtgacca acctcaccga
    36721 gctgctgccg gggctcacca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc
    36781 cattatggcg agcacgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca
    36841 aacttcgccg aaacctttgc ccgtcagagt agctgagagt ggaacttcgg tcaggctccg
    36901 gcattctcgc atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac
    36961 gttgaaaaag gccatatcac ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg
    37021 ctggaagccc tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa
    37081 gagtgcgaag cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg
    37141 acgtggatag cgggggcggt aaggtaactc gctgattaaa atatccaagg aaggaaatat
    37201 gatcgatctg gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc
    37261 ctggctagcc gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt
    37321 aggtatctcg attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat
    37381 caaaagcaaa atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg
    37441 gatgacggct gaacataaat gaatagattc gtgaatagat acttgaatgt tttttatttt
    37501 ctatccatat gaaaaataat agtaaatcta taaatcagtt gctattgatg aatagatact
    37561 aaaaagccgg agcagtcccc cactccggct taacacgaca atcgcctttc agcgaaacac
    37621 aattactctt tatcgcccag cagaacggat tccagtgcga ttttgatcat gtcgttgaag
    37681 gtggtctgac gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg
    37741 cagatggtca gggctttcgc gccaaactct gcagccacgc cgtagatacc agccgcttcc
    37801 atttccacgc cgaggatgcc gtatttttcc atcacgtcga acatttcgcc gtccggagag
    37861 tagaacaggt cagcggagaa caggttaccc acgcgagcat caacgcccag tgctttagct
    37921 gcatctactg cgttacgcac catgtcaaag tcagcgatag cggcaaagtc atggtcttta
    37981 aaacggatgc ggttaacttt ggaatcggtg caggccccca taccgataac gacgtcgcgc
    38041 agttttacgt gcggcagaac tgcgccacag gaaccaacgc ggataatttt cttcacgccg
    38101 aaatcggtga tcagttcttt ggtgtagatg gagcaggacg ggatacccat accgtgaccc
    38161 attacggaaa ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg
    38221 ttcacttcac gggcatcttc aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg
    38281 cctggcatca aaactacgtc agcgaaatcg cccatttctg cattaatgtg tggggtagcc
    38341 attgttttat cctttttgtg acataacaaa ggcggagtca acgctccgcc caaatccatc
    38401 agaacatggc tttgccatat tccatatcag aagtaccaaa atattttgcc agagtctggc
    38461 cgatatccgc gaaggtttca cggtgaccca gtgagcccgg ttttactttc gggccatata
    38521 ccagtaccgg aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt
    38581 ggtcagcggt gaggatcagg atgtcgtcat cgcgcagcag agacatcagc tccggcagac
    38641 ggcggtcgaa cagttccaga cccgcagcat aaccggcgac gtcgcggcgg tggccccagg
    38701 aagagtcgaa gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct tctttcatct
    38761 ctttgatggt ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt ttggtgatac
    38821 cgcagttggc gtagatgtct gcaattttac cgacagaaac cacctggccg tgtttttcat
    38881 caaccagttt ctgcagcacg gtcggtgctg gcggctcaac agccaagtcg tgacggttac
    38941 cggtacgctg gaagttacca gctttgtcac cgataaacgg acgagcgata acacgaccga
    39001 tattgtagcc gccgttggtc agctcttcac gggcgatttc gcacagttcg tagagtttat
    39061 ccagaccgaa agtttcttca tggcaggcaa tctggaacac ggagtcagcg gaggtataga
    39121 aaatcggctt gccggttttc atgtgctctt cgcccagttg atccagaatg accgtaccgg
    39181 aagagtggca gttaccgagg taacccggca gattagcgcg ttcgaccagt ttatccagca
    39241 gctcttgcgg gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac agaaccggga
    39301 caccggcgat ttcccagtga ccagacgggg tatctttacc ggatgacatt tcgtgcgccc
    39361 atgcgtacgc gccgataact tcagcgttgc cgtccattcc cgccggaatg aaaccggtag
    39421 aaccttcgtg tgctttcgcc agccccagac gggtcagatt tggcagattg agcggccctt
    39481 tacgaccatt atcagcttcg cctttggcac aagcttctgc gatatgaccc agggtgtcag
    39541 ccccgacgtc accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat gagtccagca
    39601 ccataataaa tgcacgtttc atatgttctc cgtactcagt gcttcaaatt taaatgatca
    39661 gatcagtata ccgttattcg ctgatacggc gatagacagt tggtgtgctt tccggtgcgt
    39721 tatcggcaag tttaattgcc gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt
    39781 ctttcgcgag gataaccgcc agcggacgct gaccgtctac ctggtcgccc agacgcgcca
    39841 tatcagtaaa gccaacgctg taatcgatgg tgtcagatgc ctgacgacga ccgccgccca
    39901 ttgcaaccac tgccatcccc agcgcgcggg tatccatttc actgacaaaa ccttcggtat
    39961 cagcatagac tgctttcgtc agcatcgctg tcggcagata cttcgcgtag ttctcaacga
    40021 agtcggtcgg gcctttttgt gccgctacca tacgaccaaa gacttctgcc gctttaccgt
    40081 tgtccagcac cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg
    40141 agatcagcat ctccacgcac agcgccatcg tgacatcaaa cagacgcggg ttacgatact
    40201 cacccgtcag gaactgcacc gcttcacgaa cttcaaccgc gttacctgca ctggaggcca
    40261 gtacctgatt catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc
    40321 caacaatcgc ttcggcaagg gcttcagaga gttcgtaggt cggcataaac gcgccgctac
    40381 ccactttcac gtccatcacc agcgcgtcca gaccttccgc cagtttcttc gccaggatcg
    40441 aggcagtgat cagcgggatg gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac
    40501 gtttatcagc cggagccagt gaactggtct gaccgataat cgccacgccg acgtctttaa
    40561 taatttcgcg gaaacggttg tcatccggga aaatgtcgaa gccagggatg gattccagtt
    40621 tgtcgagcgt accgccagta tgaccgaggc cgcgaccgga gatcatcgga atatagccgc
    40681 cgcaggctgc gaccatcggc cccaacatca gcgaagtgac atcgccgacg ccgccggtcg
    40741 agtgtttatc aacaatcggg ccattcagat gcaggctttt ccagtcgaga actgttcctg
    40801 aatctcgcat cgccatggtc agcgagacac gctcaggcat tgtcatatcg tggaagaaaa
    40861 tggtcatcgc gagggcggca atctgccctt cggaaatagt gttgtcgcga ataccgttga
    40921 taaagaaacg aatttcttca tcgctcagcg catgaccatc acgtttttta cgaataattt
    40981 cttgtgcgag aaacaaggta acctcccgag gaaaagagta tgaggcggct tttgccggat
    41041 gcgtctacgc cttatccggc ctacgggtag atacgtctcg taggcctgat aagcgcagcg
    41101 catcaggcgt aaagcatctt acttagtagc tgctggcgct cttaccgtcg ccgtggccca
    41161 gcgctttcag caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag
    41221 cccagtcagc accgaacagt tcatctgcga tggcgagata tttctgcgca tcttccgcag
    41281 tacgcacgcc gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcacggatca
    41341 cttccatcat gatgcgagcg ctttccggcg tcgcgttcac agccacttta ccggtagagg
    41401 ttttgatgaa gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt
    41461 cgtctttcag ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag
    41521 cctctttaca ggctttcacc aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc
    41581 ggtacgggaa caccacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg
    41641 ccagcgcgat ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga
    41701 tttccggggt gccctgctct ttcagagttt tgcgagcaat cgggataaaa cgaggataga
    41761 tacagatagc ggcggtattg ccgaccggag ttttggcctg atgacacaga gcaattactt
    41821 tctcgtcggt gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc
    41881 tgcttgcttt cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg
    41941 cgagtttgtt agtattctaa catctactcc gcaacacact tcgatacaca tcacaattaa
    42001 ggaaatctac ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc
    42061 aaatgaatgc gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct
    42121 tacaatggcg catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat
    42181 gcactgcaac aacgttgcca gcaaattgtc accagcccag tgcttagccc ggagcagaag
    42241 cgccattttc tggcactgga agcagaaaac aatctgcctt acccacagct tcctgccgaa
    42301 gcccgccgcg cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac
    42361 aaaccgcgct atgtcttacc cgattacgct cgttttctgg cgaacggttc cgaatggctg
    42421 gagctggaag gcgcgaaaga tcttgatgac gcactctctc tgctgaccat tctttaccac
    42481 cacgtaccgt cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa
    42541 ccgtatgtta gaattctaac acaaaacgag atcgatgttc gaataaaacg tttctggcgt
    42601 tacctcgaca gaaccctgcc agatgccttt atgcacgcca atatcggccc gtctgattcg
    42661 cccattaccc gcgcgatctt acgtgcagac gcgcaggttt caccgaacct gacctttatc
    42721 tacgatcctg aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa
    42781 tgtagcaaac cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc
    42841 tacgggattg tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta
    42901 cgccttaacc tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc
    42961 actctaccgc actactgcca gcagcagatc gccatcatcg atgcgcggtg tgaattcctc
    43021 tatcaacaat cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct
    43081 gaacgttttg tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt
    43141 gagaaagaag ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat
    43201 cgcatcagcg cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa
    43261 aaacgcgcca tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc
    43321 gcgcgtttgc cgtatggcga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg
    43381 catcatgctt attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa
    43441 cgtaacccgc aggcactggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa
    43501 tttaccgcca atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg
    43561 tcggatttag aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa
    43621 gaagccgcac gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag
    43681 cagatgcgct ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg
    43741 tctggctctg tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac
    43801 gatgggacgt tgcaatgact gtggggagtg cgtgccacag tgtccgcatc aggcgttgca
    43861 gattgttgac ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct
    43921 gaagatgtgt ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct
    43981 tagccatgtg cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagc
    44041 cacgacccaa ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact
    44101 gcgccatctc acctgtctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa
    44161 attactcccg gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca
    44221 tcaacaatta accggacgcg ataatcaaca gattaagcgc agcatctatt tgctggcaga
    44281 gcgcggcaag ctggcggaac tgcgtttgct gatgatccca ggccaggtgg attatttgca
    44341 acacatcgaa gaactggcgg cgtttatcaa gggacttggt gatgttccgg ttcgcctgaa
    44401 cgcgtttcat gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga
    44461 agacgttgag ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc
    44521 ggcgctctat ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcctg
    44581 ataagacgcg t
//
LOCUS       DAOFMX010000027        43425 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.027, whole genome shotgun sequence.
ACCESSION   DAOFMX010000027 DAOFMX010000000
VERSION     DAOFMX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.027"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            169..1272
                     /gene="proB"
                     /locus_tag="QBA20_001661"
     CDS             169..1272
                     /gene="proB"
                     /locus_tag="QBA20_001661"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDQ6292237.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1284..2537
                     /gene="proA"
                     /locus_tag="QBA20_001662"
     CDS             1284..2537
                     /gene="proA"
                     /locus_tag="QBA20_001662"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDQ6292238.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2652..2727
                     /locus_tag="QBA20_001663"
     tRNA            2652..2727
                     /locus_tag="QBA20_001663"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2685..2687,aa:Thr,seq:cgt)
     gene            complement(2742..3905)
                     /locus_tag="QBA20_001664"
     CDS             complement(2742..3905)
                     /locus_tag="QBA20_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6292239.1"
                     /translation="MSLFRRSEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDMTFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
     gene            complement(4132..4437)
                     /locus_tag="QBA20_001665"
     CDS             complement(4132..4437)
                     /locus_tag="QBA20_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292240.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            complement(4437..4799)
                     /locus_tag="QBA20_001666"
     CDS             complement(4437..4799)
                     /locus_tag="QBA20_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292241.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIDWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            complement(4790..5326)
                     /gene="yfdR"
                     /locus_tag="QBA20_001667"
     CDS             complement(4790..5326)
                     /gene="yfdR"
                     /locus_tag="QBA20_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDQ6292242.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQYAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPQVPGHA
                     YGMFMERFNELSELRKCA"
     gene            5780..5971
                     /locus_tag="QBA20_001668"
     CDS             5780..5971
                     /locus_tag="QBA20_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292243.1"
                     /translation="MTKEEKILYLFQLSVKTHTAYQTAAMTSDKNYSTSENPIDDISK
                     LYDKFEALLDKKFAEAGLE"
     gene            complement(6489..7181)
                     /locus_tag="QBA20_001669"
     CDS             complement(6489..7181)
                     /locus_tag="QBA20_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6292244.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSAHKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            7279..7539
                     /locus_tag="QBA20_001670"
     CDS             7279..7539
                     /locus_tag="QBA20_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6292245.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEVSEMQILYPARFQSSQNQNGFKPQEQEVNRG"
     gene            7532..8083
                     /gene="ymfL"
                     /locus_tag="QBA20_001671"
     CDS             7532..8083
                     /gene="ymfL"
                     /locus_tag="QBA20_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HDQ6292246.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            8259..8438
                     /locus_tag="QBA20_001672"
     CDS             8259..8438
                     /locus_tag="QBA20_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDQ6292247.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            8428..9369
                     /locus_tag="QBA20_001673"
     CDS             8428..9369
                     /locus_tag="QBA20_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6292248.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEVEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTDEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            9366..9860
                     /locus_tag="QBA20_001674"
     CDS             9366..9860
                     /locus_tag="QBA20_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDQ6292249.1"
                     /translation="MSMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTHRHFPRLTERAQDPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAVSGDEWYLSGN
                     YVGA"
     gene            9860..10513
                     /locus_tag="QBA20_001675"
     CDS             9860..10513
                     /locus_tag="QBA20_001675"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HDQ6292250.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPKDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLVQVRQQAEKLVREMAA"
     gene            10510..10836
                     /locus_tag="QBA20_001676"
     CDS             10510..10836
                     /locus_tag="QBA20_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDQ6292251.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRAAYWLH
                     ERGLKV"
     gene            10833..11222
                     /locus_tag="QBA20_001677"
     CDS             10833..11222
                     /locus_tag="QBA20_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDQ6292252.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            11242..12039
                     /locus_tag="QBA20_001678"
     CDS             11242..12039
                     /locus_tag="QBA20_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDQ6292253.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVSRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTPKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            12047..13036
                     /locus_tag="QBA20_001679"
     CDS             12047..13036
                     /locus_tag="QBA20_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDQ6292254.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVVFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            13054..13404
                     /locus_tag="QBA20_001680"
     CDS             13054..13404
                     /locus_tag="QBA20_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDQ6292255.1"
                     /translation="MRDMYEVLDRWGAWAAADNSGVDWQPVAAGFKGLLPHGKKTRQQ
                     CDDDEGIMIDSCVARMRKYKPEEYELIIAHFVIGISLRSIAKKRKCSDGSIRKQLQAA
                     IGVIEGILLIIKPL"
     gene            complement(13408..13992)
                     /locus_tag="QBA20_001681"
     CDS             complement(13408..13992)
                     /locus_tag="QBA20_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292256.1"
                     /translation="MPILSVFSHLGKPFGYLFIKGISGKAAYDWVAPILLTSITAVLF
                     ILLKIPVKDLFDDNGFIKSIVSFISNLPGFYIAALAAIATFNRAEIDLPLISNERNAS
                     IEIKVTKENGKVVNSEEVLTRRLFLCMLFSFLTTLSIVIIILNAIFSPLINVYQNSFV
                     LMVYTVVFTFLVWQLLVSTFFGLYYLGNRIHMNY"
     gene            complement(14000..15022)
                     /locus_tag="QBA20_001682"
     CDS             complement(14000..15022)
                     /locus_tag="QBA20_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292257.1"
                     /translation="MAKLNASERLVTHHSLTIDTKFRTKATQEVKAQCICPVPEMYML
                     APLIVKQKGLVHSYDSGNIVVTLQDVQLYPLLPDNSPTHIVLLINSVDKNGSTTVVKN
                     INTNERVEIQPKYEQGEGYEVSTYVVISLNGNKRTYDMICTSTPGVSTARLNSFLDKI
                     LFEVAKDNEDLFTAKHPTNVISATSKKEVKIRYKPIFEFTGMLDKELFNKISQKGLSD
                     VILVKDQFGTINAPDVNSPYIPTESTLKLLPNHGDNVIGWIKNVASHFNKKMNGGYDK
                     LKVKFQDPETNKPRQVDFKTSNINLNNLEKTFIKKSIIDNFNSRLKDSYVKIELEFVV
                     KMIDLM"
     gene            15308..15634
                     /locus_tag="QBA20_001683"
     CDS             15308..15634
                     /locus_tag="QBA20_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDQ6292258.1"
                     /translation="MPHNPNTWLELFQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGSSKE"
     gene            15638..16114
                     /locus_tag="QBA20_001684"
     CDS             15638..16114
                     /locus_tag="QBA20_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HDQ6292259.1"
                     /translation="MQALNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPRSQDSVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKEAGGVVNEVEL"
     gene            16098..16490
                     /locus_tag="QBA20_001685"
     CDS             16098..16490
                     /locus_tag="QBA20_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDQ6292260.1"
                     /translation="MKLSYKLIIAAFLLTVVCSFIWSTNHYYSKYQHEKKRADEAVQN
                     AKSATAITNNVLQSLQIVNTVLEANQHAKQQIALESQRTQADIKMAFANDDCAARPVP
                     ARAAERLRRYADSLRTSFGGTTTSESDR"
     gene            16714..17625
                     /locus_tag="QBA20_001686"
     CDS             16714..17625
                     /locus_tag="QBA20_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDQ6292261.1"
                     /translation="MIVCFYNLYRSSCWMNFMQNVQPLSLVTTLVILRSNGESKSLGT
                     GFFYQHSNGFIFLATNYHVITGISPSERGVKPFIGDSIDIQLRDKTGKLFKHTMPLFK
                     DGSPLWLEHPTDKEADIILMPLPQELFVNTGVKVVSKTTTLPNVQINPSSPVVMIGYP
                     HGYHDTTHYLPIWKTGSLASEPDIDFDGKKLIVVDISAFPGMSGSPAFYVTHSGYLTK
                     EGNMIMDMGMKIHFLGVYASMQMLNSDLFLEQVVSAAEYKVSHSESLQLGHVWKAELL
                     EEIAATSSPIATTPSQFQSNSKFLFKY"
     gene            17701..18051
                     /locus_tag="QBA20_001687"
     CDS             17701..18051
                     /locus_tag="QBA20_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDQ6292262.1"
                     /translation="MPPRTPKACRVRGCRSTTTDPSGYCKQHKGEGWKQYKPGQSRHQ
                     RGYGSKWDVIRARVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADRNLQSLC
                     WPCHKAKTARERLK"
     gene            18177..18671
                     /locus_tag="QBA20_001688"
     CDS             18177..18671
                     /locus_tag="QBA20_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDQ6292263.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            18668..20401
                     /locus_tag="QBA20_001689"
     CDS             18668..20401
                     /locus_tag="QBA20_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDQ6292264.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     SRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTDPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFANMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDLVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            20413..20595
                     /locus_tag="QBA20_001690"
     CDS             20413..20595
                     /locus_tag="QBA20_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292265.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            20595..21836
                     /locus_tag="QBA20_001691"
                     /pseudo
     CDS             20595..21836
                     /locus_tag="QBA20_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            21814..22464
                     /locus_tag="QBA20_001692"
     CDS             21814..22464
                     /locus_tag="QBA20_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDQ6292266.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            22479..23684
                     /locus_tag="QBA20_001693"
     CDS             22479..23684
                     /locus_tag="QBA20_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDQ6292267.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEELDRNILNLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTSGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDTEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            23734..23934
                     /locus_tag="QBA20_001694"
     CDS             23734..23934
                     /locus_tag="QBA20_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292268.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            23937..24260
                     /locus_tag="QBA20_001695"
     CDS             23937..24260
                     /locus_tag="QBA20_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDQ6292269.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTDVEKMELPMSFNWLVGPY
                     RYFPQ"
     gene            24257..24667
                     /locus_tag="QBA20_001696"
     CDS             24257..24667
                     /locus_tag="QBA20_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDQ6292270.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VAFRTWAKVVQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGNNDDFLFAR"
     gene            24642..25148
                     /locus_tag="QBA20_001697"
     CDS             24642..25148
                     /locus_tag="QBA20_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292271.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            25145..25705
                     /locus_tag="QBA20_001698"
     CDS             25145..25705
                     /locus_tag="QBA20_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292272.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLDPGNGPDGDIEHHTEIPLPS"
     gene            25714..25884
                     /locus_tag="QBA20_001699"
     CDS             25714..25884
                     /locus_tag="QBA20_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HDQ6292273.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            25868..27364
                     /locus_tag="QBA20_001700"
     CDS             25868..27364
                     /locus_tag="QBA20_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDQ6292274.1"
                     /translation="MTISFNTIPSNTLVPLFYAEMDNQAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATV
                     TLTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIKDAINAVPTLPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKTGTLSELVNAG
                     DQFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNSYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            27364..27720
                     /locus_tag="QBA20_001701"
     CDS             27364..27720
                     /locus_tag="QBA20_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDQ6292275.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            27720..27989
                     /locus_tag="QBA20_001702"
     CDS             27720..27989
                     /locus_tag="QBA20_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDQ6292276.1"
                     /translation="MKELELKKPIIAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            28131..29966
                     /locus_tag="QBA20_001703"
     CDS             28131..29966
                     /locus_tag="QBA20_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDQ6292277.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKVMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYPAVDPAQSNNASDVTGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            30027..31355
                     /locus_tag="QBA20_001704"
     CDS             30027..31355
                     /locus_tag="QBA20_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HDQ6292278.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKVTFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSGAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGPSTTPAQSTGWPFVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            31352..32431
                     /locus_tag="QBA20_001705"
     CDS             31352..32431
                     /locus_tag="QBA20_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HDQ6292279.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGVERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            32431..32979
                     /locus_tag="QBA20_001706"
     CDS             32431..32979
                     /locus_tag="QBA20_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HDQ6292280.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     IEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPKARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            32979..33404
                     /locus_tag="QBA20_001707"
     CDS             32979..33404
                     /locus_tag="QBA20_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HDQ6292281.1"
                     /translation="MELWLTVNGKRTCASAPLDPLIRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            33391..34449
                     /locus_tag="QBA20_001708"
     CDS             33391..34449
                     /locus_tag="QBA20_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDQ6292282.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGEADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIAGSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHKVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            34440..35024
                     /locus_tag="QBA20_001709"
     CDS             34440..35024
                     /locus_tag="QBA20_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDQ6292283.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSARDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPVATNTTWMTCGD
                     PCDSALRFWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            35028..35678
                     /locus_tag="QBA20_001710"
     CDS             35028..35678
                     /locus_tag="QBA20_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292284.1"
                     /translation="MHRIDTKTAKKDKFVAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            35650..36084
                     /locus_tag="QBA20_001711"
     CDS             35650..36084
                     /locus_tag="QBA20_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ6292285.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(36857..37890)
                     /locus_tag="QBA20_001712"
                     /pseudo
     CDS             complement(36857..37890)
                     /locus_tag="QBA20_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            38341..39531
                     /locus_tag="QBA20_001713"
     CDS             38341..39531
                     /locus_tag="QBA20_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HDQ6292286.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDRLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLETGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(39921..41381)
                     /gene="gtrII"
                     /locus_tag="QBA20_001714"
     CDS             complement(39921..41381)
                     /gene="gtrII"
                     /locus_tag="QBA20_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HDQ6292287.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILIFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IHGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(41378..42307)
                     /locus_tag="QBA20_001715"
     CDS             complement(41378..42307)
                     /locus_tag="QBA20_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDQ6292288.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(42304..42666)
                     /locus_tag="QBA20_001716"
     CDS             complement(42304..42666)
                     /locus_tag="QBA20_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDQ6292289.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(42928..>43425)
                     /locus_tag="QBA20_001717"
     CDS             complement(42928..>43425)
                     /locus_tag="QBA20_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDQ6292290.1"
                     /translation="EWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLH
                     KRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWASWL
                     VQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEENVV
                     YLNAR"
BASE COUNT        11061 a        10048 c        11413 g        10903 t
ORIGIN      
        1 gtggtaattt attaaatctg taataaaagc gtaaacaact gccgctacgc ttgctgatcc
       61 cgcgcaacaa aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta
      121 aaacgttcgt ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc
      181 cagacgctgg tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac
      241 cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg
      301 attgttattg tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa
      361 ctgccagcga ctatcgcctc gaaacaactg ctggcggcgg tagggcagag tcgactgatt
      421 caactgtggg aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc
      481 cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg
      541 ctcgataaca atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt
      601 aaggtcggcg ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa
      661 ctgttgctgc tgaccgatca aaaaggtttg tataccgctg atccgcgcag aaatccgcag
      721 gcagaactga ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac
      781 agcgtttcag gcctcggaac tggcggcatg agtactaaat tgcaggccgc tgacgtggct
      841 tgccgtgcgg gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat
      901 gtgatggaag gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac
      961 cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca
     1021 actgccgcca ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact
     1081 ggcaatttct cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc
     1141 cacggtgtca gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa
     1201 gaaattgatg caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg
     1261 attacccgtt aaggagctgg ccgatgctgg aacaaatggg cattgccgcg aagcaagcct
     1321 cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg
     1381 atgaactgga agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg
     1441 cgcgagccaa tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc
     1501 tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg
     1561 taatcgatgg cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg
     1621 gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt
     1681 gcctgaaaac cggtaatgcg gtgatcctgc gtggtggtaa agaaacctgt cgcactaacg
     1741 cggcaacggt ggcggtgatt caggacgccc tgaaatcctg tggcttaccg gcgggtgccg
     1801 tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat
     1861 acatcgacat gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt
     1921 cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg
     1981 cagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat
     2041 gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat
     2101 taagcaaaca aatggcggaa agcggcgtga cattacacgc agatgcagct gcgctgacac
     2161 agttgcaggc aggccctgcg aaggtggtgg cggttaaagc cgaagagtat gacgatgagt
     2221 ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata
     2281 ttcgtgaaca cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc
     2341 agcgttttgt taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta
     2401 ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg
     2461 cgcgtggccc aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt
     2521 acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc
     2581 cattgacgca tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat
     2641 gtcctttcag ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg
     2701 taggttcgac tcctattatc ggcaccattt aaatcaataa gttacacatc attagtacct
     2761 tccttatttt ttgactggga caaatttggg accgatgggt tcaggatcga gtctatttgc
     2821 cgtgcatgtt cggtaaggtg attaggtgcg aggtgagcat atcgacgaac catttcgata
     2881 gactcccagc ctcccatttc ctgtaacact gacaacggga ctccggcttg aaccagccaa
     2941 cttgcccagg tgtgtctcaa gtcgtgaaat ctgaaatcat caatacctgc tcgtctcagc
     3001 gccgctttcc aggctgtgtt tgcgtcatac cgcatcttcc tgaccgttgg cgctttcgtt
     3061 ccgtctggtt tggtacagct ttccttgtaa acaaataccc aacggtgatg attcccgatt
     3121 tgttttttca atacgcgaca tgcagtatca ttcagcgcaa cgccaattgc gcggtttgat
     3181 ttactctctt ccgggtttat ccatgccacc cggcgctgca tatctatttg ttgccattca
     3241 aggttgatga tgttcgagcg tcttaagcct gttgccagtg caaattcaac aacagacttt
     3301 aatggctccg gacattcatc aatcagcctt tgtgcttcat ggggctccag ccagcggatc
     3361 cgtttattct ttggttgagg cactttaata attggtgcct tatccagcat tttccattca
     3421 cgctctgcgg ctcttagtag ggcctttata aatgaaagat gcgtagcctt cgttgcaacg
     3481 gacgctggtt ttggcgtgta ttctggaaca ggtttccctt tttttctgca tgcttctgcc
     3541 ctgagtttcc agttttcctc atgacgccgg ttcgtcattt tctgcattgc tgaataaatt
     3601 tttgattcag tgatgtctct tagttgcatc cctgcgaaat gttgaagcca gaatccgatc
     3661 cggcttttgt catcgtccag tgatttctta tgtgctttct cttcgagcca cctgacacac
     3721 gcttcctcaa acgtcatatc aggtatttca ccaagtttgc tgacccgcca tgcttcagcc
     3781 tttagcttgt catggagctc tgtcgcctgc cttttgtcct ttgttccaag agactgttta
     3841 aatcttttac cgttcggcaa tgtgaaactg gcgtaccata tttcacttct gcggaagagt
     3901 gacattttct ttcctctgtt atgccatcac ccgcgctcac ctggacagta tgcagcggag
     3961 actgaagagc cgcaatgcag gcttgtcgtg ttgtgaggta aggagattta ttcttagtgg
     4021 gatctttgcg tgttgcctga agacgccctg tgcgtatcca gttaatggca gtcggtctgg
     4081 atatcttgag aaaatgacag gcctcatcga gtgtgaggct gtatggctcc attatttcac
     4141 ctcttgttgt gacattgttg aaaaatggat accagctcgt tgctgccaga cgatccaacc
     4201 gagagtcata tcccatgcca tgtattcgtt atcgccgttt tttgctctcc gacgatctac
     4261 taagtcaccg aaacgctttt ccatgaataa ttcataagct tcgcgttcat ctggttctac
     4321 ttccagagat aggagtgcga tttcataagc acggcgctca atatcgtctc gcacgtcaag
     4381 gctgctgata cgctctttaa tttctttaat cagttctttg tcggtaaaag tggtcattat
     4441 gctccagcct ccggtgcttt tggcattact gcccagtgag tgatattgac gttttcaagg
     4501 tccccgacct gaaatgtcca ctgccattct ccggtttctt tttgtcccca ggtgtaccag
     4561 agagaacgcc agtcaattag ccagccttct ccgttagcat cgaataacaa aacactttca
     4621 tttgctggtg gcagttcagt tgacactggt attactttgt tttcctgtgc tgcacattta
     4681 gcttcaagcg catcgaattt acgcaccagg tattcagcat ctgtttcatt cactttcaga
     4741 tctcgcggta cacatctccc acgaagaaac ccttccattt cgaaaacatt catgcgcatt
     4801 tgcgtaactc cgataactcg ttaaaacgtt ccataaacat cccgtaggca tggcccggta
     4861 cctgtggaat cactttgaac atctctgttg ccgggatacc ttccagtaca ggccagaaag
     4921 agccatcatc aagcccaaga tcgcggcgtt cggttgccag cataatgaga tcggcatatt
     4981 tcactggcgt gctcataaca ggaggtaacc cgtatttctc acggattacg gcgtctattt
     5041 tttcttccat ccgtttatag tcaggaagaa gtcgtttcag tggtgcgggg atgtcctggc
     5101 aatatgcttc tgttgcatca tgcattaaag cttcaaaagc aaattcctgc ggcaccagct
     5161 ggctgcaaag caccgcatat tgggcgacac tgtagaagtg tgaaagatgt cctgcaaagc
     5221 gacagatatt tgaaagggaa accgcgatat cgttaataac gatgtcgtct ttatttatcc
     5281 tgtcataata aaaatgcttc ccggaaaaag ttttaataaa tgacattttg ttctccacgt
     5341 atatgcgctg caccgcgctg aattcgggta aaaggaagcc ctcaccgtcc ggcgattatt
     5401 gagtcaatta catttccata aatgcccccg taggggcggt tagtttctcc acaaaacaga
     5461 gaagaacacc tgcggtggca gccgctcgga tggattgggt tatgagcccg tcgtccggtg
     5521 atgctcttct ctgttttgta aaaaggacgg taccagccgg aagcaatggt acaaactggt
     5581 accgccagga ctacacacag cataaagttg tggtgccggg tgcctcccgg tgcctggcga
     5641 aggttgcaca ccagacgggt gggtatccac agaaggtcga ctgtcagcct caaccttaac
     5701 ccgcgtgcgc tgagccgcat tcaccacttc aggctcggtg gtatcttggt gttttcatat
     5761 agccaagaag gaaatagtta tgaccaaaga agaaaaaatt ctttatttat tccaactatc
     5821 ggttaagact cacactgcat atcagactgc tgccatgaca tcagataaaa attacagtac
     5881 gtcagaaaac ccgatagacg acataagcaa gctttacgat aagttcgaag cactactcga
     5941 taaaaagttt gctgaggctg ggcttgagtg attgttgaat aatcgacaaa acccaactta
     6001 aattttcgtc agtgggctcg atgccatgtg cggtgagctc acttttcaaa actccaagta
     6061 attcagagct gattttcaat atatctgctt gatttctaac tattcccact ttttcctccc
     6121 ttggtctacg cgcggtcatg ttttacgccc aaacgacttc acagttattg tttaaaatct
     6181 ggactttcat ttcatacacc tgctttaaca tgagtgcctg gtggcacaac atgactcaac
     6241 gaatcatcct ggacttcata tgccccaggc ggctacttcg tgggcgtcct gcctgttcgt
     6301 ttttgacatt tactgactgc ttacgacaca tgcaccgtgt tgcaaccaga ttttgttgta
     6361 atcctgtagt tggtctggaa caaaagataa aattaaattg cgagatatgc aagtgatatt
     6421 tgcgatatat gcaaatttat aggtaataaa aagccacctt tcggtggccg atggatggga
     6481 tattgaggtt aattatgtct cttaagggtt tgcgactgac tgattaagac ctttccaaag
     6541 accatgaatc ggtgttcgtt ttcgctagta attccccatt cacggtaaat ctggttatca
     6601 gaaatcacca gcagtttgtc aggaatcatt tgaagtcttt taacatatat tttgtcatca
     6661 aaaccaaaga catatatacc atcaccatca aactgattga tgctgacatc aacgaagatg
     6721 agatctcctg gctcaatggt tggacacata ctgtccccac gaacgttgat aactttgatg
     6781 tgattggctg gtcgtccgcc gaacattgat acagcattat cagttctgta ttcgatggca
     6841 tgaatcacat caatgacatc accgccctgg ataaggccat ttcccgcact tgcactgata
     6901 tccagcattt caatacggaa cacatccttc acctgcgcaa catcctcatt attactgttt
     6961 ttatatacag tattactttt gtgggcagag gtaaagagat cagcaatatc aacacctaag
     7021 ctcttggcaa tattactcag tgtttgttcg gtaaattgtt tttgcttacc cgtttctaag
     7081 cgcgagatgt tcgccgcatc tactcctatt gcttcagcga gatcggcgat tttcatgttc
     7141 ttcgcttggc gaagttgtct gactcggttt cctatgttca tgcgtttatt acatttcttt
     7201 attgcgtgat aagcaaatca acttgcgcaa aataattgcg tgaaataaca tgcataacgc
     7261 gcaatatttg gagggcatat gcaatcacca ttacgaaatg tgcgtaaggc gcatggtttc
     7321 actttgcagc atgttgctgc gggtgttcaa gtcaatccag cgacgttgag tcgtattgag
     7381 agactggagc agattccatc tatcgagctt gcagaacgat tagccaattt ttttaagggt
     7441 gaagtcagcg aaatgcagat tctttatccg gcacgttttc aatctagcca aaaccagaat
     7501 gggtttaaac cacaggaaca ggaggtgaac cgtgggtaag catcactgga aagtagaaaa
     7561 acagcctgag tggtacgtga aagctgtcag aaaaactatc gcagcgttgc cgggggggta
     7621 cgctgaagct gctgactggc tggatgtaac agagaacgca ttatttaacc gccttcgtgc
     7681 cgatggcgat cagattttcc cgctgggatg ggcaatggtt ttacagcgtg ctggtggcac
     7741 tcacttcatt gctgatgctg tggcgcagtc tgcaaatggc gtctttgtgt ctcttcctga
     7801 cgtcgaggat gtggacaacg ccgatattaa ccagcgtctg ctggaagtca ttgaacagat
     7861 cggcagttat tcaaaacaga ttcgttcagc aattgaagac ggtgtagtgg aaccgcatga
     7921 gaagacagca attaacgacg agctgtatct ctcaatttcg aagctgcagg agcatgcagc
     7981 actggtctac aaaatctttt gcatttcaga aagtaatgac gcccgcgagt gtgcagctcc
     8041 gggcgccgtg gcgtgtcgtg actgtggaga aactaacgca tgaacagttt aacaacacac
     8101 taccgtcgct cgcaactgat tgcgcttcct gtaccgggtg gaaaagcgaa ggtggagtat
     8161 tgctatgcag tgaatgtacc aggtgacagg gaaattgtaa cccacagctt tgcagagtgg
     8221 gctgtgggtg atttcaaccg gcagaaggag acagtccttt gcgacaagtt aaccgctggt
     8281 tcaaagatca ctacggagtg cccgtcagag tcattcgttg ggagccggaa acacaacgag
     8341 ttatctacct ccgtgaaggc tatgagcatg agtgcttcag cccgctcgaa cagtttcgtc
     8401 gtaaattcag ggaaatagag gtcggtcatg agcactaaat taaccggcta tgtatgggat
     8461 ggttgcgcag cgtcaggcat gaaattatcc agcgtggcaa ttatggcccg cctggctgat
     8521 ttcagtaatg acgaaggtgt gtgctggcca tcaattgaaa ccattgcccg tcagattggc
     8581 gcggggatga gtaccgtcag gacggctatc gcacggctgg aagtagaagg ctggttaacg
     8641 cgtaaggcgc gtcgccaggg taaccgcaat gcgtcgaatg tttatcagct taacgttgcg
     8701 aagcttcagg cagcggcatt ttctcaattg tcagattctg acccgtcaaa atctgacgca
     8761 tcaaaatctg acccgtcaaa atttgatgcg tcgaaatcag gcaaaaaagc gggttttcac
     8821 ccgtcagaat ctggcgggga tccgtcagta aaatcaaaac atgatccgtc agataaaaaa
     8881 ccttctcgtc cggacgcttc gcaaccggac acgcagacgg atgaacagga ttttttaact
     8941 cgccatcctg atgcggttgt attcagccct aaaaagcgcc agtgggggac gcaggatgat
     9001 ttgacctgcg cacagtggct ctggaaaaaa atcatcgccc tgtacgagca ggctgccgaa
     9061 tgtgacggcg aggtggttcg tcccaaagaa ccgaactgga cagcctgggc aaacgaaatt
     9121 cgcctgatgt gtgtgcagga tggtcgtact cacaaacaaa tctgcgagat gtacagccgc
     9181 gtcagccgcg atccgttctg gtgccgtaac gtgctcagcc cgtcgaagct gcgggaaaaa
     9241 tgggatgagc tttccctgcg cttatcgccg tccgtcagca cgtacacaga aaaacgcgaa
     9301 gacccgtact tcaaagccag ttacgacaat gtggactaca gccagatccc ggcaggattc
     9361 agggggtgag catgagtctt ttgaatgacg ttcagaaatt cattgaagcc catccgggct
     9421 gtacttccgg agacattgcg gatgcttttg caggttactc acggcagcgc gttctgcagt
     9481 cagcaagcaa gttacgtcag agtgggcgtg tggctcaccg ttgtgaagga gatacacaca
     9541 gacatttccc gcgcctgact gagagagcgc aggatccgga accacaacca gttcgtgaaa
     9601 ccagacctgt gcgcaatttc tatgtcggca ctaacgaccc gcgggtgatt ttgtgcctga
     9661 cccgccaggc ggaagaactg gagtccaggg gcttataccg tcgtgctgca acggtgtgga
     9721 tggcggcatt ccgtgaaagc cactcccagc cagaacgaaa caattttctg gcgcgtcgtg
     9781 aacggtgttt acggaaaagc agcaagcgcg ctgtatcagg tgatgagtgg tatctgtcag
     9841 ggaattacgt gggggcttaa tgagtaataa atattgtcag gcactggtgg aactgcggaa
     9901 caaaccagcc catgaactga aggaagtggg cgatcagtgg cgcacgccag ataacatttt
     9961 ctggggaatt aacaccctgt ttggtccgtt tgttctggat ctgttcactg acggtgataa
    10021 cgccaaatgt gccgcgtatt acacggcgga agacaacgcg ctggcgcatg actggtcaga
    10081 acgtcttgcg gagcttaaag gtgctgcctt tggtaatccc ccatacagcc gcgccagtca
    10141 gcatgagggg caatacatca ccggcatgcg ttacatcatg aagcatgcca gtgccatgcg
    10201 tgataaaggc gggcgctatg ttttcctgat caaagctgcc accagcgaag tgtggtggcc
    10261 gaaagacgca gaccatattg cttttattcg cgggcgtatt ggttttgaac tgcctgcctg
    10321 gtttatcccg aaggatgaga agcaggtgcc gacaggcgct ttcttcgctg gtgctattgc
    10381 tgttttcgac aagacctgga agggaccggc aatcagctac atcgggcgcg atgaacttga
    10441 ggcatgtggt gaagcctttc tggtgcaggt tcgccagcag gcggaaaaac tggtcaggga
    10501 gatggcggca tgacgacgtt aactcaatgc cagcagcagg tgctggatat gctgatttct
    10561 taccagaaag agcgtggctt tccgccaacc aatcaggagg tggcaaccat gctgggatac
    10621 cgttcagtga atgcagcggt agagcatctt cgtgcactgg agaaaaaagg cgtcatcacg
    10681 ataaagcgtg gcgtggcccg ggggataacg cttcataccg cggtgaagga cgacgacagc
    10741 gaggcggtcg ggattatccg cgcactgctt gccggtgagg aaaacgccag gctgcgtgca
    10801 gcctactggt tacatgagag aggcctgaaa gtatgaagct gatcctgcct ttcccgccca
    10861 gcgtgaacac gtactggcga caccccaaca aaggggcgtt tgctggtaag agtctgataa
    10921 gcgcggcggg gcgaaaattc cagagcgcgg cgtgcgcagc aatagttgag cagttacgtc
    10981 gtctgccgaa accaacgtcg gcacctgctt cagtggagat cgtgttgttt cctccggata
    11041 accggatccg cgatctggac aactataaca aggcgctgtt tgacgccctg acccacgcgg
    11101 gtgtgtggga agacgacagt caggtgaaaa gaatgctggt ggagtgggga ccggttatcc
    11161 cggaagggaa ggtcgagatc actatcagta agtacgagaa aacggcgggt gcagccgcct
    11221 gatcaagagg agaaacgaag tatgaataat ctgatggtca ttgatggtat tgaagttcgt
    11281 cgtgatgctt atgggcgtta cagcctgaac gatctgcaca gggctgctgg ttctctggat
    11341 aagcataagc ctgcattctg gctccgcaat gagcaaaccg aacgtttaat aagcgagttg
    11401 cagatttgca actcggtcaa tatagagcca gttaacgtta gtcgtggcgg aaataaccag
    11461 gggacgtatg tctgcaaaga actggtgtat gcctatgcaa tgtggatcag cccgtcattc
    11521 catctgaagg tgatccgtac tttcgatatg gtaaccagcg caccggaaaa attatccggg
    11581 caggctgctg acaagatgca ggctggcgtg attctgctgg actttatgcg ccgggagtta
    11641 aacctgtcta actcatctgt gcttggggcc tgtcagaaac tccaggaggc tgttggctta
    11701 ccgaatctgg caccgcgcta tgccattgat gctcctgctg acgcgcctga tggctcaagc
    11761 cgccccacgc tgtcgctgag tgcactgctg aaacagtatg gtatccgcct gacggctaat
    11821 caggcatatc accagatggc gaagctgggg atcgttgaac aacgcgaacg atacagccgt
    11881 accgcgatta acaacatcaa aaaattctgg tcgctgacac cgaaaggctg catgttcggc
    11941 aagaacatca ccagtcccgc aaatccgcgc gagacgcagc cgcacttctt cgaatcccga
    12001 ttccctgagc tgttaaagct gctcgatacc gttcattgag gtgaccgtga gagcactact
    12061 gacccctgaa attgccccgc gtatggggat cgtattgttc aggccaggtt cagagctgat
    12121 gcccctgttt atgcaggggc gtgtcctgct ggagcctgag ccggaacgtt attcatcttt
    12181 cgccagtggt gccgttccgg ctgcatcaca accgctggcg gatgatcctg ccgttcgggc
    12241 cgtgttccgc aatgaggcag tgatccgtcg tgctggtggc gtggaatgtc ttgaaagctg
    12301 gttacttcgt gaaaaaggct gccagtggcc tcattccgac tggcacagcg agaacatgac
    12361 cacaatgcga cacgctccgg gcgcaattcg tctgtgctgg cactgcgata accagctgcg
    12421 cgatcagttc acggaacggc tggaatcaat ggcaacggat aactgtgccc gctgggtgtt
    12481 gtctgtcgtg cgtcgggatc tcggttttga tgatagtcac gttgtgacaa tgccggaact
    12541 gtgctggtgg ctggttcgta atgacctggc tgatgcctta ccggaaagtg cagcccgtaa
    12601 ggcactgaga ttaccgaagc ctgttgtgcc gtctgttacc cgggaaagtg accttgtgcc
    12661 ttcggttcct gccaccagca tcatccagga taaggcgaaa aaggtgctgg cgctgaaagt
    12721 ggatccggag tcgccggagt cttttatgtt acgcccaaaa cgtcgccgct gggttaatga
    12781 aaagtacacg cgctgggtta agacgcagcc gtgtgcatgt tgtggaaagc ctgctgatga
    12841 tccccaccac ctgataggcc acggtcaggg gggaatgggt acaaaagcgc atgatctctt
    12901 tgtgttgcct ttgtgcagaa agcatcatga cgagctgcat gcggataccg tggtatttga
    12961 agagaagtat ggctcccagc tggagctgat atttcgtttt atcgatcgtg cgctggcaat
    13021 tggcgtgctg gcctgatttt gtggagaagg ttgatgcgtg atatgtatga agtattggac
    13081 cgctggggag catgggctgc agcagataac agtggtgtgg actggcagcc tgttgctgca
    13141 gggtttaaag gtttactacc tcatggaaag aaaacacgcc aacaatgcga tgatgatgaa
    13201 ggaatcatga ttgacagctg tgttgcgcga atgcggaaat ataagccaga agaatatgaa
    13261 ctgatcattg ctcactttgt tatcggtatt tcactccgtt caatagcaaa gaaacggaaa
    13321 tgctctgacg ggtcaatcag aaaacaactt caagcagcaa taggagtgat tgaaggtatt
    13381 ttgttgatta ttaagcccct gtagggctta ataattcata tgaattctgt ttccgagata
    13441 atataatcca aagaaagttg atactaagag ttgccaaacg aggaaagtaa atacaacagt
    13501 ataaaccatt aaaacaaaac tgttttgata gacattgatt aatggtgaaa atatagcatt
    13561 aagtattatt ataacaatgc ttaatgttgt taaaaaagag aatagcatgc aaagaaataa
    13621 tcgtctggtc agaacctcct ctgaatttac tacttttcca ttttcttttg ttacttttat
    13681 ttcaatagag gcatttcttt cattgctaat caatggtaaa tcaatctcag ctctattgaa
    13741 agttgctatg gcggccagag cggcaatata aaaccctggt aggttagaga taaatgaaac
    13801 gattgattta atgaaaccat tgtcatcaaa taaatctttg acagggattt ttaatagaat
    13861 gaaaagtact gctgtgatag aagtaagtag aattggtgct acccaatcat atgcagcttt
    13921 gccgctaata cctttaataa aaagatatcc aaacggcttg cctagatgag agaaaactga
    13981 aagtattggc ataataacct cacatcaaat ctatcatttt cacaacaaac tctagctcaa
    14041 ttttaacata tgaatccttc aggcgcgaat taaagttatc gataatgctt ttcttaataa
    14101 atgttttttc taaattatta agattgatgt ttgaggtttt gaaatcaacc tgccttggtt
    14161 tatttgtttc aggatcttgg aatttaacct ttaatttatc ataaccacca ttcatttttt
    14221 tattgaaatg agatgctaca tttttaatcc atccaataac attgtcacca tgatttggaa
    14281 gtaattttaa tgtactttca gtcggaatgt aaggtgagtt aacatcagga gcattaattg
    14341 ttccgaattg atccttgact aatatgacat ctgataatcc tttttggctt attttattaa
    14401 aaagctcctt gtctaacatt cctgtaaatt caaatattgg tttgtatcgt atttttactt
    14461 ctttttttga tgttgcagaa ataacgttag ttggatgttt tgcggtaaat aagtcttcat
    14521 tgtctttcgc cacttcaaag agaattttgt ctaaaaaact atttaaccgg gccgtagata
    14581 cacctggggt agatgtacaa atcatatcat aggttctttt atttccattt aatgatatga
    14641 caacataggt agaaacttca tagccttcac cttgttcata ttttggttgg atttcaacgc
    14701 gttcattagt atttatattt tttacaacgg tagtacttcc atttttatca acactattaa
    14761 taagaaggac aatatgcgtg ggtgagttat ctggaagtaa aggataaagt tgtacatctt
    14821 ggagagtaac tacaatattc ccagaatcat atgaatgaac gagtcccttt tgtttaacaa
    14881 tcaatggggc aagcatgtac atttccggaa caggacatat acactgggct tttacctctt
    14941 gtgttgcctt ggttctaaac tttgtgtcaa tggtcagact atgatgagta acgagtcttt
    15001 cgctagcatt taattttgcc atgaaatcat ccgattatga tttttcgatt tatttgttaa
    15061 tcttacaaaa acgctaacgc gtacgcaaaa attattgtat cgtgttaaga gtggttactt
    15121 cgccacacag cttaaacccg ccgtcgagcg ggttttgtcg tttctggggc tggggattcg
    15181 ttggtcctgg tctattccgc agttatccat tggttcggct tctttgacgt ttccgcttct
    15241 aatttgctgt acatgatgtt ccctcaattt gcacctgctg tatcagcgag gtgagagata
    15301 actacaaatg cctcataacc caaatacctg gctggagttg ttccagagct ggtggcgtgg
    15361 agacacaccg ctgggcgcag tgattatgtc gatcgttatg gctggcttgc gcattgccta
    15421 ttttggcggt ggtggtggct ggaaacgaaa aacgctcgag attttgctct gtggtgctct
    15481 gacgctgact tttgcatccg ctcttgagta tgtcggatgg cctaaatctc tttctgttgc
    15541 cattggtggc ggtgttgggc tgattggtgt cgatgcaatt cgtggggctg caatgcgagt
    15601 aatcggtaac aaatttggta gctcgaagga gtaatttatg caggcactaa attcccagcg
    15661 taaagctttc ttggatatgg ttgcatggtc agaaggaacg gataacgggc gacaaccgac
    15721 acgtaaccac ggttatgatg tcattgtcgg tggtgaactg tttactgatt actccgatca
    15781 tcctcgcaaa cttgtcacgc taaacccgaa actcaaatca acagcagccg gacgttacca
    15841 gcttctttcc cgttggtggg atgcctaccg caagcagcta gggctgaaag acttctctcc
    15901 cagaagccag gactccgtgg cattacagca gattaaagag cgtggcgctt taccgatgat
    15961 tgatcgcggt gatatccgtc aggcaatcga ccgttgcagc aatatctggg cttcactgcc
    16021 gggcgctggt tatggtcagt atgaacataa aatcggtgac ctgattgcca ggtttaaaga
    16081 ggctggtggg gtggtaaatg aagttgagct ataagctgat tatcgctgct ttccttttaa
    16141 ctgtcgtctg ttctttcatc tggtcaacca accactacta cagcaaatat cagcacgaaa
    16201 agaaacgtgc tgatgaggct gtacaaaatg ccaaatcggc aactgccatt actaataacg
    16261 tcctgcaatc actacaaatc gtcaatacag tactggaggc taaccagcat gcaaaacagc
    16321 agatcgcact ggagtcacag agaacccagg ctgatatcaa aatggctttt gcgaatgatg
    16381 attgcgctgc ccgtcctgtt cctgctcgtg cagctgaacg gctgcgccgg tacgcggaca
    16441 gtttacgtac cagtttcggc ggtaccacta ctagcgaatc tgaccgctga aactccgcaa
    16501 ccagctatcc ccgacccgct gacatggagt gggagcctgg atttgaacgt aagcctgttg
    16561 tcggcgctgg cgtcttgtaa tagggataag ttagacatta gaaagagtga gcacctgctt
    16621 tcggagaaaa actctgctga aaagtgaggt tatatagcta gatcggaatt ggcgattcat
    16681 atatcggaaa tagttggtta agaattttct ggaatgattg tatgctttta caacctatat
    16741 cgaagttcat gttggatgaa ttttatgcaa aacgtacagc cgctatctct ggtaactact
    16801 ttagtaattt tgaggtctaa tggagagtca aagtcgttag gtactgggtt cttttatcaa
    16861 catagcaatg gtttcatttt ccttgcaact aactatcatg taataactgg aatcagccca
    16921 tccgaaaggg gtgtaaagcc atttataggc gatagtattg atattcagct gagagataaa
    16981 accggaaaac tattcaaaca tacgatgcct ctgtttaaag atggttcacc gctgtggttg
    17041 gagcacccca cagataaaga ggcagacatt atcttgatgc ctttgcctca agagttgttt
    17101 gtaaacacag gagtaaaagt agtctcaaaa acaacgacac tgcctaatgt ccagataaac
    17161 ccctcatctc ctgttgtaat gattggatat cctcatggtt atcatgacac gactcactac
    17221 cttccaatat ggaaaacagg aagcttagcg agtgagccag atatcgattt cgatgggaag
    17281 aaattaatag ttgtcgatat ttctgctttc cctggaatgt caggctctcc ggcattctat
    17341 gtcacacaca gtggttatct tacaaaagaa gggaatatga tcatggatat gggaatgaaa
    17401 attcatttcc ttggcgttta tgcaagtatg caaatgctaa acagtgatct tttcttagaa
    17461 caagtcgtta gtgctgcaga atacaaagtg tcgcatagtg aatctttgca actagggcat
    17521 gtctggaaag ctgagttact tgaagagata gctgcgacat ctagtccgat tgcaaccaca
    17581 ccatctcaat ttcaaagtaa tagcaaattc ttatttaagt actgagatac gtctacacat
    17641 aaaccgcctg ttggaggttt ttatgcatat cgtgcgagca gtatcagaga gaatatttca
    17701 atgccaccac gaacaccaaa agcctgccgt gttcgcggct gccgatcgac aaccacagac
    17761 ccgtcaggtt attgcaagca acacaaaggc gaaggctgga agcaatacaa gccaggacaa
    17821 tcccgtcatc agcgcggtta tggttcgaag tgggacgtta tccgcgcgcg tgtgctgaag
    17881 cgtgacaaag gcctgtgtca gttgtgtttg cgtgccggtg tggtgcgcga ggcgaaaacc
    17941 gttgaccaca tcattcctaa agcgcatggc ggcactgatg ccgaccgtaa tctgcagagt
    18001 ctgtgctggc cgtgtcataa ggcgaagacg gcccgtgaac ggttgaagtg ataataattc
    18061 tcaactgctt gaggggaggg gcgggtcaaa tccctgcggc ctgacgtctt ccggactgcc
    18121 cgccccatcg tttttttata cccgcgaaaa atgaaattta accaggagtg ccgcatatgg
    18181 ctggaacggc ggggcgttcc gggcgtcgcc ccaagccaac ggcgcgcaag gcgctggccg
    18241 gaaaccccgg caagcgagcc ctgaacaaag atgaacctgt ttttacgccc atcaaaggtg
    18301 ttgagccacc ggagtggttc gctgaagaag atctccctct cgccacgatc atgtggcaac
    18361 tgacaaccaa agaactctgc ggtcagggcc tgctgtgcgt gactgacctg gcggtacttg
    18421 agcggtggtg cgtggcctat gagttctggc gacgtgccgt gaaaaatatt gccatacagg
    18481 gcaacaccat caccggtgca atgggcggca tggtcaaaaa tccggagctg accgccaaaa
    18541 aagaacagga gtccgagatg agcagcacgg gtgcaatgct cggactcgac cccagcagcc
    18601 gccagcgtct gattggcctg gcggggcaga agaaagccac taacccgttt ctgaaaatca
    18661 tcgagtcatg agccggaaat cttaccccaa cgtaaatgct gccaatcagt atgcccgtga
    18721 tgtcgtgcgc ggaaagattg tggcctgcca gtttgtgatt caggcctgcc agcgccatct
    18781 tgatgacctg atggcggaaa aaagtaagtc gtttcgttac cgcttcgaca aagacctggc
    18841 tgaacgggcc gcgaaattta ttcagctgtt gccacacacc aagggggagt gggcattcaa
    18901 acggatgccc atcacgctgg agccgtggca gctctttgtg atctgctgcg cgtttggctg
    18961 ggtcaataaa ggctcccggc tgcgccgctt ccgtgaggtg tataccgaaa tcccccgtaa
    19021 gaacggcaaa tcggcaatct ctgccggtgt cgccctgtat tgttttgcct gtgataacga
    19081 gtttggcgcg gaagtgtatt ccggtgccac gacagagaaa caggcgtggg aagtctttcg
    19141 cccggctcga ctgatgtgta aacgcacacc catgctgacg gaagcgttcg ggattgaggt
    19201 taacgcctca aacatgaacc gtccggagga tggcgcgcgg tttgaaccgc tgatcggtaa
    19261 ccccggtgat ggttcatcac cccactgtgc ggtggtggat gaatatcacg agcatgccac
    19321 cgatgcgctt tataccacaa tgcttaccgg gatgggggcg cgacgtcagc cactgatgtg
    19381 ggccatcacc accgccgggt acaacattga ggggccgtgc tacgacaaac ggcgggaagt
    19441 catcgagatg ctcaacggct cggtgcctaa cgatgaactg ttcgggatca tctataccgt
    19501 tgatgaaggt gacgactgga ccgacccgca ggtgctggaa aaagccaatc caaatattgg
    19561 cgtgtcggtt tatcgcgaat ttttgttaag tcagcagcag cgtgcgaaaa ataacgcccg
    19621 tctggcaaac gtctttaaaa caaaacacct caatatctgg gtgtcggcgc gttcggcgta
    19681 tttcaacctg gtgagctggc agagctgcga ggataaatca ctgactcttg agcagttcga
    19741 ggggcagccg tgcattctgg cctttgacct ggcgcgtaag ctggatatga acagcatggc
    19801 gcgactttat acccgcgaga ttgacggtaa aacgcattac tacagtgtgg ccccgcgttt
    19861 ctgggtaccg tatgacacgg tgtatagcgt cgagaaaaat gaagatcgcc ggacagccga
    19921 acgctttcag aaatgggtgg aaatgggcgt tctgaccgtt accgatggtg cagaggtgga
    19981 ttatcgctac atcctcgaag aggccaaagc ggcgaacaaa atcagcccgg tcagtgagtc
    20041 acccatcgac cctttcggag cgaccgggct gtcacatgac cttgctgatg aagacctgaa
    20101 tcccgttact atcgtccaga acttcgccaa tatgtccgac ccgatgaaag agctggaggc
    20161 agcgattgaa tcgggacgct ttcatcatga cggcaatccc atcatgacct ggtgtatcgg
    20221 caatgtggtc ggcaaaaaca tgccaggtaa cgatgattta gtgaagcccg tcaaagagca
    20281 ggcggaaaac aaaatcgatg gtgcagttgc gctgattatg gcggttggca gagccatgct
    20341 gtacgagaaa gaagacacgc tgtctgacca cattgagtcc tatgggatcc gctcgcttta
    20401 actgaggtaa ttatgatcat gctgattctc gcgcctctgg tgggcgtgct gggggcgctt
    20461 ttgctggcgt atggtgcctg gctgatttat cccccggcgg ggtttgttgt tgccggggcg
    20521 ttgtgtctgt tctggtcgtg gctggtggcg cgatatctcg accgtacaca gtcgtctgtc
    20581 ggcggaggta aatagtgttc ttttcgggat tatttcaacg aaaaagtgac gcaccggtga
    20641 ccacgccagc agagctggcg gatgctatcg ggttgtccta cgacacctat accggaaagc
    20701 agatcagcag ccagcgggcc atgcgactga cggcggtttt ttcctgtgtc agggtgctgg
    20761 cggagtcggt cgggatgttg ccctgcaacc tgtatcacct gaacggcagt ctgaagcaga
    20821 gagccgctgg cgaacgtctg cataagctga tctccacgca tcccaatggc tatatgacgc
    20881 cgcaggagtt ctgggagctg gtggtcacct gtctgtgcct gcggggaaac ttttacgcct
    20941 acaaagtgaa agcatttggc gaagtggctg aactgctgcc cgtcgatccc ggctgtgtgg
    21001 taccgaagct taacagtagc tgggagccgg tctatcaggt cacattcccg gatggctcca
    21061 cggatgtact gagctaggag gatatctggc atgtgcgcac gctgacgctg gacggactgg
    21121 tggggctgaa tcccatcgcc tatgcccgcg aggcaatatc gctggcggca gcgaccgaag
    21181 agcacggggc cagactgttc agcaatggcg cggtgacgtc gggtgtgttg cgtacagagc
    21241 agacgctgtc agatcaggct tatgagcgcc tgaagaaaga ttttgaggag cgtcacaccg
    21301 ggcttggcaa tgctcaccgc ccgatgatcc ttgagatggg gctggactgg aagtcgatgg
    21361 cgctgaacgc cgaggacagc cagttcctgg aaacccgcaa gtttcagctt gaagaaatct
    21421 gtcgtctgtt ccgggtgccg ttgcacatgg tgcagaacac cgatcgcgcc accttcaaca
    21481 atatcgaaga gctggggctg ggatttatca actattcact ggtgccgtat ctgacccgca
    21541 tcgaacagcg gatcaacacc ggactggtac gaaaaagtaa gcagggcgtt tattacgcca
    21601 aatttaacgc cggggcgtta ctgcgcgggg atatgaagtc ccgttttgaa gcctacgcca
    21661 ccgggatcaa ctggggaatt tactctccca atgactgccg cgacctggaa gatatgaatc
    21721 cgcgtcccgg tggtgatgtc tatctcacac cgatgaacat gaccacgaaa ccctccgatg
    21781 gcagtaaagc cggtaagcag aaggataacg ccaatgcaga cgaaacaacg tcttgatgta
    21841 ccgctgagtc tgaaatctgt cagtgactcc ggtgagtttg aagggtatgg ctccgtcttt
    21901 ggtgtaaagg acagccacga tgatgtggtg atgtccgggg catttgctgc ttccctgcgg
    21961 gcgtggagtg acagaaaagc gttacctgcg ctgctctggc agcaccgcat ggatgaaccc
    22021 atcggtgttt acaccgaaat gaaggaagac gatgtcgggc tttacgtcag gggacggttg
    22081 cttattgatg atgatcccct cgcaaaacgc gcacatgcac acatgaaggc cggttcgtta
    22141 accggccttt ctattgggta cgtcctgaaa gactgggaat acgaccggag caaagaagcc
    22201 tttctgctga aagaaatcga cctctgggaa gtcagcctgg tgacgttccc gtctaacgac
    22261 gaggcgcgga tcagcgacgt caagaacgca ctggcccgcg gggaaatccc cgaacagaaa
    22321 aaaatcgaaa gagtcctgcg tgatgtcgga ctctcccgta cccaggccaa agcattcatg
    22381 gccgggggct atggcgcact gtccctgcgc gacgctgagg atgtgggctc tgcactgaat
    22441 gcactgaaaa atctgaactt ctaatcagga gaaatacgat ggcggttgat attaaagatg
    22501 tcgaacaggt cgcgcaggag ctgcagcaga agtttgacga cttcaaagca aagaacgaca
    22561 agcgcgtgga tgcgattgag caggaaaaag gcaagcttgc cgggcaggtg gaaaccctga
    22621 acgggaaact cagcgagctg gaaaatctca aaagcgacct tgaaaaagag ctgcttgagc
    22681 tgaaacgtcc ggctggtgga gcgcaaaata aactggccac cgagcataaa gaggcgtttg
    22741 tgggcttcct gcgtaaaggc cgtgaagacg gtctgcgcga tctggagcgt aaggcattgc
    22801 aggtgggtac cgatgaagac ggtggctacg ccgtgccgga agaactggat cgcaacattc
    22861 ttaacctgct gaaagatgaa gtggtgatgc gtcaggaagc cacggtgatc accgttggcg
    22921 gttccgacta caaaaaactg gtgaatctgg gcggtacggc ttccggatgg gtgggggaaa
    22981 cggatacgcg atcccagact gccacctcca gactggagct gattgaacct ctcatggggg
    23041 aaatttacgg caacccgcag gctacccaga aaatgctgga cgatgccttc ttcaacgtgg
    23101 aggcctggat caacagcgag ctggcaaccg aatttgccga acaggaagaa attgccttta
    23161 cctcaggcga tggcaccaag aagccgaaag ggttcctggc gtatgaatcc actgatgaaa
    23221 ccgacaaggt ccgggcgttc ggcaaacttc agcatattgt atccggcgaa gcgaccgcgg
    23281 tgaccgcaga cgccattatc aaactgattt acacgctgcg taaggcacac cgcactggcg
    23341 cgaagttcat gatgaacaac aacagcctgt ttgccatccg tctgctgaaa gacaccgagg
    23401 gtaactatct gtggcgtcct gggctggaac tggggcagcc atcctctctg gcgggttacg
    23461 gtatcgctga aaacgagcag atgccggata ttgccgcgga tgcgaaagcc attgcatttg
    23521 gtaacttcaa acggggttac accatcgttg accgtatcgg tacccgcatt cttcgcgatc
    23581 cgtacaccaa taaaccgttt gtcggttttt ataccaccaa gcgcaccggc ggcatgctgg
    23641 tcgattcgca ggccatcaaa ctgctgaaga ttgctgcggc gtaatcactc aggggcgcgg
    23701 aaccgcgccc cctgttctga cgggtgaaga atcatgatcc tgaaacaaga tctgaaatgg
    23761 tcaccggacg gtatgcgtgt tgaggtcatt cgggccggtg agtatgacga cggggcgctt
    23821 cctgcccggg tgcaggagat tgcacttcag gccgggttag cagagcgcgg aaccagtgca
    23881 aaaagcagta aagcgacaaa agagaaaaaa gccacgacca gtaaagaggg ctgagtatgc
    23941 ttctgacaat ggaagagatt aaagcccaac tccggctgga tgaggatttc gatgctgatg
    24001 accgccatct gcaactgctg gcctgtgcgg cgcaaaagcg gacggaaacg tatctgaacc
    24061 ggaagctcta tgctccggat gaaaccattc cggacagcga tccggacggg ctgcacctgc
    24121 cggatgatat tcgtctgggg atgctgatgc ttatcagcca tttttacgaa aatcgctcgt
    24181 cggttacaga cgttgagaaa atggagttgc caatgagctt taactggctt gtcggcccgt
    24241 acaggtattt cccgcaatga aaattcgtca ggcgcagacc agcgcaacct acattctgcc
    24301 ggaccccggt gaactgaata aacgcgtcct gatccgccag cgggtggata tgcccgcgga
    24361 taactttggc gtggagcctc aatacccggt tgcgttccgg acatgggcga aggttgtcca
    24421 gaccagtgcc accacctggc aggaaaccgc gcagaccggg gacgccatca cccattacat
    24481 caccattcgt taccgccggg ggatcaccgc tgattatgag gtggtctgcg gtgacagtgt
    24541 gtaccgggtg aaacgtcagc gcgatctgaa cggggcgcgg cgctttctgc tgctggagtg
    24601 tacggagctg ggcgaatgta ggcagagtca cggaggcaac aatgacgact tcctttttgc
    24661 acgttgattt tcagcagcct gcggagatgc gctttaaccg cgcccgtgtc cggcgggcgt
    24721 ttgtcacgat tggtcagcgt catatgcgtg atgcccgtcg gctggtgatg cgccgtgcgc
    24781 ggtcggcacc gggtgaaaac cccggttatc agaccggacg cctggctcgt tcgattggtt
    24841 acatggtacc cagagccagt aaacatcgcc ctggttttat ggcacgtata gcccctaacc
    24901 agcgtaatgg agagggaaac cgccgtatca ccggtgattt ttatccggct tttttgttct
    24961 atggcgtgag gcgaggggca aagcgtcgtc gcagccatca tcgtggtgca tccggtggca
    25021 gcggctggcg actggctcca cgtaataact tcatggtgga aactcttgaa aagaaccgca
    25081 gctggacacg ctattttctg gcgcgggaat tgcgtaaatc actgaagccg gagcgacgac
    25141 acagatgaaa ctgacgcctg ttattgctgc gctgcgtgcc cgctgcccgt attttgaaaa
    25201 ccgggtggca ggcgcggcac agttcaaaaa tctgccggag gtcggaaagc tgagactccc
    25261 ggcggcgtat gtggtaccgg gggatgattc tccgggagaa aacaaaagcc agaccgacta
    25321 ctggcaggag ctgaaagagg gcttctccgt ggttgtcata ctgagtaacg ggcgtgatga
    25381 gcgcggtcag tttgcttcgt atgatgtggt ggacgatgtc cggcagatgc tctttaaggc
    25441 tctgctgggc tggaacccgg aagcgtgcgg taacccgatt acctatgacg gcggcacgct
    25501 gctggatctg aatcgtcatg agctgattta tcagttcgat ttttcggtca tcagcgagct
    25561 gaccgaagac gatacccgcc agcaggatga cctgaacagt ctggatgaac tgcgaacgct
    25621 ggcgattgat gttgattatc tcgatcccgg taacgggcct gacggcgata tcgaacatca
    25681 caccgaaata ccccttcctt cctgaggatc atcatgtttg tgaaacctgt taaagggcgg
    25741 tcagtgcctg accctgcccg cggcgacctt ttgcccgccg aagggcgaaa tgttgatgag
    25801 aacaactact ggctgcgccg tgaagcagcg ggtgatatcc ggcgcgtgaa taaaaaggtg
    25861 aacaccgatg acgataagct ttaacaccat tccgtcgaat acgctggttc cgctgtttta
    25921 tgcggaaatg gataaccagg ctgcgaatac tgcacaggac agcggagcat cgctgctgat
    25981 tggtcatgcc aataacggtg cagagattgt tgccaacagt ctggtgctga tgccgtcggc
    26041 agactatgca cgccagattt gtggtgcggg aagtcagctg gcgcgtatgg tcgaggctta
    26101 tcgccagacc gacccgtttg gtgagctgta tgtgattgcc gttccggaag ccacaggcgc
    26161 ggcggcaacg gttacgctga cggtgaccgg ggaagcaacc gaaagcggca cggtgaatgt
    26221 ctatgtggga cgtacccgcg tgcaggctcc ggtgaccaac ggcgataacg tcacgacgat
    26281 tgccagcagt atcaaagatg ccatcaatgc cgttccgact ctgccgttta cagcttcatc
    26341 ttcggctggc gtggtcacac tgaccgcgcg tcataagggg ctttgcggga atgaaattcc
    26401 tgtcagcctc aattactacg gcttcggtgg gggcgaagtg ctgccagcgg gcgtacagat
    26461 tgccgtggcg acggggaccg ccggaacggg cgctccggtt ctcaccggcg cggtggctgc
    26521 aatggcggat gagccgtttg attatatcgg tctgccgttc aacgacacgg cctccgttaa
    26581 cacgctggtg accgagatga acgataccag cggtcgctgg agctatgcgc gtcagctgta
    26641 tggtcatgtg tatacggcaa agaccggcac actgtcagaa ctggtgaacg caggtgacca
    26701 gtttaaccag cagcacatca ccctggcggg gtacgaaaaa gagacccaga cgcctgccga
    26761 cgagctggcg gcaagccgta ccgcccgcgc agcggtgttt atccgcaacg atccggcacg
    26821 tcccacgcag accggtgagc tggtgggtat gctgcctgcg ccgaagggga aacggttcac
    26881 gatgaccgag cagcagaccc tgctgtctca tggcgtggca acggcgtatg tcgaaagcgg
    26941 ggtgctgcgc attcagcgtg atgtcaccac gtacaggaaa aattcttacg gggttgcgga
    27001 taacagctac ctcgacagcg agacgctgca taccagtgcg tatgtactgc gcaaactgaa
    27061 atccgtcatt accagtaagt acgggcgtca caagcttgcc agcgacggta cccgctttgg
    27121 tcccggtcag gcgattgtca ccccggcggt aatcaaaggg gaactgctgg caacctaccg
    27181 tcagcttgag cgtgcgggga tcgtggaaaa ctacgaactg tttaagcagt acctggttgt
    27241 ggagcgtgat gccagcgatc cgaaccgcct gaacacgctg ttcccgcctg actatgttaa
    27301 ccagttgcgt gtttttgccg tggttaacca gttccgtctt cagtattcag aggagtctgc
    27361 ataatggccc gtatcggggg aacctgttat ttcaaaattg acggtcagca gctatcgctg
    27421 accggcggca ttgaggtgcc catgaacagg acggtcaatg atgacatcat cggcctggac
    27481 ggttcagtgg accgcaagga aactcaccgt gcgccttatg tcaaagggac attcaaggtg
    27541 ccgaagaatt ttccggtgag taaaatcacc tcgtctgatg agatgaccat cactgccgag
    27601 ctggcgaacg gtcaggtcta tgtattgtcg tccgcctggc tgcacggaga agcgaaccat
    27661 aatgccgaag aaggcacggt tgatcttgag ttccacggtg aagaagggga ttaccagtaa
    27721 tgaaagagct tgagttaaag aaaccgatta tcgctcatgg tgagacactc tccgtactgg
    27781 agtttgatga acccaccggg aaggatgtcc gcgagctggg gtatccctac cagatgaatc
    27841 aggatgagtc cgtcagactt ctggcgcatg tggtgtcgaa atacattgtg cggctggcga
    27901 aagtgccgca aagctctgtc gaccagatgt ctccggcaga cctgaatgca gcggcgtggc
    27961 ttgtggctgg ttttttcctc caggcctgac ggctgaatac ctcactgatc gcttctttga
    28021 ctgcgccagc tactggcgca ttaatccctt cgaattgctg aatatgccga tcagtgaaat
    28081 tcccttgctg gtcagtcagg caaacaggat agagcaggag aaacgcacac atggctgaat
    28141 ttgagcttaa ggcgttgatc accggtgtcg acaggctttc tcccgcgctg tcgaaaatgc
    28201 aaaagaaaat ccggggattt aaacgccagg cggaagaagc gtcacagggt gggctggcgc
    28261 ttggtggcgg actggcagct ggtctgacgc tttccctgaa atcttatgcc gatcaggaaa
    28321 acgccgccac cgggctgaaa gtcgccatga tggatgcgaa cggcgaggtt ggaaagagct
    28381 ttcaggacat caataaactg gctattggac tgggtaacca gctacccggt acaacggctg
    28441 atttccagaa catgatgcag atgctggtgc gtcaggggat cccggcagaa aacattctgg
    28501 gtggtgtggg taaagcgaca gcttatcttg cggtacaact gaaaaaaaca ccggaagcgg
    28561 ctgctgagtt tgctgcaaag atgcaggatg ctaccggaac ggcgtcagaa gacatgatgg
    28621 ggctgttcga cactatccag aaggcgtttt atctgggcgt tgacgatacc aacatgttgt
    28681 ccttcttcac taaaaccagt tctgttctga agatggtgaa caaggacggt cttcaggctg
    28741 cacagagcct tgcccccatc agcgtcatga tggatcagat ggggatgaac ggggagtcgg
    28801 caggtaatgc actgcgaaaa gttatccagt ccggattaag tgttaagaaa atcagggacg
    28861 ttaataaagt tatggcccgc cagaaactcg gggtacagct cgattttact gacggcaaag
    28921 gaagttttgg cggtcttgat aacatgttca ggcaactggc aaagctgcga aaactgaccg
    28981 acgttaagcg aacaggtgta cttaaggcaa tatttggtga tgatgccgaa acccttcagg
    29041 tggtcaatgc actaatcgat aaaggaaagg atggctacga tcagatccag cagaagatga
    29101 ataaacaggc cagcctgaat aaacgtgttc aggcacagct tggtacgctg tccaacctgt
    29161 gggaggcaat gacagggacc gcaactaacg gccttgcggc tattggcggc gcattttctg
    29221 gtgacgctaa aaatatcacg caatggctgg gggagttggg ggagaaattc acgaagtttg
    29281 cggatgaaaa tccccgggtt attcgcggcg tcgtcgggct tgctgccggt cttgcgattc
    29341 tgaaactggg attgatgggt gttggcggtg ccatcagtat tgtcagcagg atcatgtcga
    29401 tgacgccgat tggcatgatt gcgacggcga tagccctggc tgcgggatta attatcacta
    29461 actgggatgt tgtcggacct tattttaaga aactctggga aaccattggt ccttattttg
    29521 aggctggctg ggaactcctt aagaaagttt ttgcctggtc gccgctgggg atggtgatca
    29581 ataactgggg gccggttgtt aagtggtttc aggatatgtg ggacaagctg aagccaatta
    29641 ttgagtggtt taccgacagt tccggtgaca cggtcgatgc cattaactct gcgcagtggg
    29701 gcgcgggtgc ttatgatgct tatgggacgg gaataccggc gcggggatac acaccttatc
    29761 cggcggtaga tccggctcag tcaaacaacg cctccgatgt cacaggcccg aaccccttca
    29821 tgattaacaa agcttctgcg ccaaaagttg atggtgagat caaggtctct tttgtgaatt
    29881 cgcctccggg tatgcgggtt atggaaacgc gatccagcgg ttttgatgtc agccatgatg
    29941 ttggctatac gcgctttggc aggtaatgaa aaattaatct gttaatgagt cccactccgg
    30001 tgggattttt tatgtacgga gtttatatga cgtggaaaga cagacttcag gacgcgtcat
    30061 ttcgcggtgt gccgtttaag gttgaagaag aaagtgcggg aaccggtcgt cgtgtggaaa
    30121 cgcacgaata cccgaaccgc gacaaaccct ataccgaaga tctgggaaaa gtcactttcc
    30181 gcccgtccat cacggcttat gtggtgggag atgactgctt tgaccagcgc gatcgcctga
    30241 ttgacgcgct gaataaaccc ggtcccggca cgcttgtcca tccgacatac ggtgagctga
    30301 aagtctgtgt tgacggggag gttcgggtca gcacatcgaa gagtgaaggg cgtattgtcc
    30361 gctttgacct gaagtttgtc gaagcgggag aactctctta ccccacatca ggtgcggcga
    30421 cggcgcagac gctgatgtca tcctgttctg cactggatga ctgcatcagt gacagtttca
    30481 gtggtttcag tatcgatggc gtggcagatt ttgtgcagaa cgacgtcgtc ggtaatgcca
    30541 gcacaatgct tgggtatgtt tctggtgcga tgaaagtggt ggattctgcc gtatcggatg
    30601 ccgccaggct gttgcagggg gatatctcgg tacttctgcc gccgccatcg tcaggcaaaa
    30661 atttcgttga gcaggtgcag aaaatgtggc gtaccgggaa acgcctttat ggtaacgcca
    30721 gcgacctggt caccatgatc aaaacgcttt ccggtgtcag cctcggcagc gatctgcaac
    30781 cgcgcggcgt ctggaaaacg gacagtaaaa ccaccgccac ggcgacgcag cagcgtaacg
    30841 tggttgccag cacccttcgt acgaccgcaa tcagcgaagc ggcgtatgcc gtcacacgat
    30901 tgcctgcgcc cacaacttcc gcggtgatgc agaatgccac agtggggccg tcaacaacac
    30961 ccgcgcagag caccggctgg ccttttgtca cgcatccggc actgaacaat gcaccggcgg
    31021 tgaaaaacac ggttgacctg ccaacgtggg aagaactgac cgacattcgc gacacactga
    31081 atacggcaat tgataaggag ttgtcccgta caaccagtga tgcgctgttt ctggcgctgc
    31141 gccgggtgaa agcagatctg aatgcggata tcaacacgcg ccttgaacag tctgcacgga
    31201 tcattcagcg cacgccggat gaggttttac ccgcgctggt gctggcggcg acctggtttg
    31261 ataacgcggc gcgtgacgcg gacattatcc ggcgtaatgc cattacgcat cccggctttg
    31321 tgccggtgat ccctctgaag gtgccagtgc aatgaacgat aacgtcacgc tacgggtaaa
    31381 tggccgggag tggaatggct ggacatcggt gcgcatcggt gccggtgttg aacgactggc
    31441 gcgggatttc agtgtggaga tcacccgcca gtggccggga gatgagggta tcaccacgct
    31501 tcagccgcgc attaaaaacg gttcaaaagt ggaggtgctg attggtgatg agctggtgat
    31561 caccggctgg gtggaggcga cgcccgttcg ttacgatgcc cgttcggtca gcaccggtat
    31621 tgccggacgc agtctgaccg ctgacctgat tgactgtgca gccgaaccga cacagtttaa
    31681 cggacgatcg ctggtacaga ttgcgcaggc gcttgctgcg cctttcggca ttgaggtggt
    31741 gaacagcggt gcgccgtcgg gtgttattcc tgatgtccag cctgatcacg gtgaaacggt
    31801 gattgaggta atcaacaaaa tactcggtca gcagcaggcg ctggcttatg acgacccgca
    31861 cggcaggctg gtgattggcg gtattggctc aacgcgggca cataccgcgc tggtacttgg
    31921 ggaaaacatc ctttcctgtg atacggagaa gagtatccgg gagcggtttt ctgtttacca
    31981 ggtggcgggg cagcgtgccg gaaacgacga tgatttcggt gaggccacca ccaccgcgct
    32041 gcgggcccgc acagaggacg catttattgc ccgttaccgt ccgatgtata tcaggcagac
    32101 agggcaggct acgggggcag gctgtattgc gcgtgctgac tttgaagccc ggcaacgggc
    32161 ggcgcggacg gatgaaacca cctatgtggt gcagggctgg cgacagggta acggtacgct
    32221 gtggcagccc aaccagcggg tgattgtctt tgatccggtc tgtggtttcg acaataccga
    32281 actgcttgtt tcggaagtca cgtttactca ggaccagaac ggcaccctga cggaaatccg
    32341 tgtcggcccg cctgatgctt atctgcctga acccgaagcc cccggcgcgc ggaaaaagaa
    32401 aaaagcaaga gtacaggagg acccgttctg atgaggacga ttgaagccat gcagcgacaa
    32461 ctcctcggcc tgattgggcg ggcagtggtg aaaagcatca gtgccgccac gaaatgtcag
    32521 accgtggatg tgtccctgat tgccggtgaa cccaaagccg ggattgaaca tcttgaaccc
    32581 tacggtttta ccgcaagggc aaacagcggt gcggaagcgg tggtgttgtt tccggatggc
    32641 gaccgttctc atgcggtggt tgttacggtg tcggaccggc gctaccgcct gaaagggctg
    32701 cagacgggtg aggtggctgt ctatgacgat caggggcagt ctgtgacgct gacccgggag
    32761 gggatcgtgg tggacggtgc aggtaaaacg atcacgtttc gcaatgcgcc taaggcacgt
    32821 tttgaaatgg acctggaagt gacaggacag gtgaaagacc tgtgcgactc cggcggcacc
    32881 accatgtcag cgatgcggct tgcctataac gggcatcgtc acagagagaa cggtcaaggc
    32941 agtaacaccg acaaacctga taaagcgatg gaggcatgat ggaactgtgg ctgacggtga
    33001 acggtaaacg cacctgcgcc agcgcaccgc tggatccgct gatccgcgcc gtggtgattt
    33061 ccctgtttac ctggcggcgt gcggagcctg atgacaacgc cgacgtcccg atgggatggt
    33121 ggggggatac ctggcctgcg gtacagaatg accgttacgg ctcccgactg tggctgcttc
    33181 agcgcagcaa actgaccaat cagctggtgc agacggtaag ggggtatatc cgcgaatgcc
    33241 tgcaatggat gattgatgac ggcgtggtgt cccgtattga tctggatatc cgccgcaccg
    33301 ggattaatga actgggtaac agtatcactc tctggcgtcg tgacggaccg gtaatgattt
    33361 cttttgatga tctgtggagt gcgataacgc atggcggaca gtgaatttca gcgcccgacg
    33421 ctggcagaaa atatcagtat gctccgtaac gatttattcg ccaggctgga cgtcagcgac
    33481 acgctccggc gcatggatga agacgtgcgg gcaaaggtgt atgcggcggc gctgcatacg
    33541 gtttacggtt acatcgatta tctggcaatg aacatgctgc ctgacctgtg cgatgagtcc
    33601 tggctggcgc gacatgctgc gatgaaacgg tgtccgcgca agggggccac ggctgccagc
    33661 gggtatatgc gctgggaagg tgtcagtgat ggcctgaagg tgaccgccgg gagtgttatt
    33721 cagcgcgatg acctggttca gtacacggca actgccgatg caaccagctc cggtggtgtc
    33781 ctgcgcgtgc cgatcgcctg ctcaagtgca ggtgcggtcg gtaacgctga cgacggtacg
    33841 gcattaatcc tggtcacgcc ggtgaatggt ctgccgtctt ctggcgaggc agataccctg
    33901 acaggcggat ttgatactga agagctggaa acgtggcgcg cccgcgtcat tgagcggtat
    33961 tactggacgc ctcagggcgg ggctgacggg gactatgtcg tctgggctaa agaagtgccc
    34021 ggcattaccc gcgcatggac ataccgacac tggatgggaa cggggactgt cggtgtgatg
    34081 attgccggca gtgacctgat taatcccatt ccggaagaat caacggaaac ggcggcaaga
    34141 caacatatcg ggccactggc tccggtggca ggctctgatt tgtatgtatt caggccggtg
    34201 gcgcataaag tggattttca tatccgtgtg acgccggaca caccggaaat acgggctgcc
    34261 atcaccgccg agttgcgttc gttcctgctg cgtgatggtt atccgcaggg agaactgaag
    34321 gtgtcacgta tcagtgaagc gatttccggt gcgaacgggg aatacagcca tcagttgctt
    34381 gcaccggcgg acaatatctc cattgcaaaa aatgaactgg cggtactggg gacgatttca
    34441 tggacgtgac aaacgatgat tacatccgtc tgttgtcggc actgttgcca cccggtccgg
    34501 cgtggtcagc cagagatccg gcgattgccg gtgcggcacc gtcattaacc cgcgttcatc
    34561 agcgtgcgga tgccctgatg cgggagctgg atccgcgcac caccactgaa ctgataaacc
    34621 gctgggagcg tctgtgcggt ctgccggatg aatgtattcc cgcagggaca cagacccttc
    34681 gccagcgtca gcaacgactg gatgcgaagg ttaacctggc gggcggcatc aatgaggatt
    34741 tttaccttgc acagcttgct gccctgggca gaccagacgc taccatcacg cgatacgaca
    34801 aaagcacgtt cacctgctca tctgcctgta ctgacgcggt gaatgcgccg gaatggcggt
    34861 attactggca ggtcaacatg ccagtcgcca ccaacaccac ctggatgaca tgtggcgatc
    34921 cctgtgattc cgcgctgcgc ttctgggggg acaccgttgt cgagtgtgtg cttaacaaac
    34981 tctgcccgtc gcatacctat gtgattttta aatatccgga gtaatccatg catcgtatag
    35041 acacgaaaac cgcgaagaag gataagttcg tcgcgggtaa gaacggtttt acccgtggta
    35101 acccccagac tggcacgcct gccaccgatc tggatgatga ctactttgac atgttgcagg
    35161 aggagctttg cagcgtggtg gaggcatccg gtgccagcct ggagaagggg cggcacgatc
    35221 agttgcttac cgcacttcgc gcgctgctgt taagccgcaa gaatccgttt ggcgatatca
    35281 aatcggatgg cacggtgaaa acggctctcg aaaaccttgg tttgggagaa gcagctaaaa
    35341 ggaatgtagg tacaggggcg aatcagatac ctgatatgag cctgttcgcg tcaattaata
    35401 ccgtaacggc tgctgcgcaa aaatttccgt ctggattaat tttacagtgc ggtcagttga
    35461 atggtgcccc gaatgtatct tcaacatacg ggatgaggtt cccgatgacg ttctcaagag
    35521 taattgctgt cgtagttaca ttgaacgtta ctggcgcggc tgggcagccg actgtatcgg
    35581 cgacaagtgt ccagaacact ggatttaata ttactgtgtc gcccggttca ggatacggtt
    35641 catctgctga tgcgtattac attgcaatgg gatattaaca aaatgtcata tttttattct
    35701 gcatcgacaa acggatttta ttcgactgaa tttcacggca ccaatattcc tgatgatgca
    35761 gtggaaatct cggaatcaga gtggaaaaca ctgattaatg cacagagtgt aacaaaaatg
    35821 attacctgtg gtgagaacgg tcatcctgtc attgttgacc gtccttctcc aacaccagaa
    35881 caattagcct taataaatga tgaaaagaaa tctgcactga tagcagaggc aacgaatgta
    35941 atagctccgc ttcaggatgc ggttgattta ggtatggcaa cagatgatga aacgaaactg
    36001 ttactggcat ggaaaaaata tcgggtgctg cttatgcgtg ttaatgtagt aaaacccgag
    36061 tggcctatgc atccaaataa atagacttgt taatctagga taattgttgg aataatactc
    36121 atttttcagt aggttcaggt agcctgggta taatgctcac ttctacaatg atttcaactt
    36181 attttattta attgatatta cgaaacttaa ctcatacata aacaatatga ctcaatactg
    36241 tcctaaatag ctgcgcagaa aagtgctcca ctttcgctca gcccttgctg ggcgaagcat
    36301 caggaaggcg ttctcagcta ggttgtgtta tatctggctt gagttttgtt gcccatacgc
    36361 aacacattac ccatctgtaa acgacaagtg tggcagaatt aggtgcagct atttagacta
    36421 tatcgacgca gtaacgataa tagatatcga aactgtacta gatattagct acttatggtt
    36481 gccgggggga gtcttttgag cactggtgtt atcaatcggc ttcaaataat tcaccaataa
    36541 attatattgc gatagctcat caccttccag gttgtttata gccagtttat cagcgtcacc
    36601 acctactgtt tatgctttta tttctttgat taataagtat atgatatttt aattcatctg
    36661 gtgtatttct ttctcatgtg ttggtcgact gaatatctgt tcagtgaagg ggtaatttta
    36721 tgtttgcact tcgaacattc ccttcagttg ctaggaaaga aaaccgtaac atttgcgcat
    36781 taattttaac agtataatct gagcataaat atgcgctata tatctataaa aaggcgcaat
    36841 taattgcgcc tttttatcat tttatcaaaa atggcccacc gactgtcctc gatagccaat
    36901 ggccaatttt atctgataca tatacacaaa tacaggtata tatgattgag cagataggat
    36961 ttggaatagt cacgctgaag cctataattg ctaaagccat agggataaat aatttggtga
    37021 tatactccat tccacatatg ttaagagtat ttcttccagc tcttacaata aagtcatttc
    37081 cacaaattat ttttgctatg aaaatattaa agattatcaa gcctaccgtg gtgataatag
    37141 tattaatagc ttgaatataa ctacttgata ttatgtctgc attgaatatt gagataatct
    37201 taaaaagtaa aggtttctgg ttaagtagtt gatacgcact cattatagcg gtaattgaga
    37261 agacaatgaa accaatttta gattttccaa aaaacctgtc acgtgttaat tctaaaaaca
    37321 tacatctacc gagaggcaat aaccaccagt atgccatagc ggagtctatg ttccaaaacc
    37381 attgaggatc tagtaatggg ttatgattga gcactgtttg tgacaacatg aacgataaca
    37441 gtgatatgat taaaatcact atattgttct tgactatttc tctaaagatt gcatcaattg
    37501 ctattataac aaaaaggcag tttataaacc atattgttcc tacgaattgg ttatttctaa
    37561 caccatatat tatatcataa atgtggttgt atatataatc aatcgtctca cctgagttaa
    37621 ttgtgtttat tattaatata gatatggaat cattaatctg taaaactttg acttgatata
    37681 atcgaaaata gataagtcat tttttttgat agtaaaaaaa ccagcagcga aaaagaatag
    37741 tgggacgtga taactaaaaa caaaaggata caatttgcca gcccccaacc caaggtgacc
    37801 taaatatata gcaaaaatac cgagaaattt taaagcatca acccaatcat gtcttatttt
    37861 tctgttgctg tcgattttgt tattatacat ttaaagtagg ctctccagtt ttgtgtgatt
    37921 tttaaacact cattgcaaat atgaatgcct gaaagtttat gattattaat caacgagttc
    37981 aatattaatt tattattttt caaagtcgca ctatataggc atttacttcc gcagtaccac
    38041 cgatagtatc gatatcaaat cagcctgcgg ccgcgataac tttcgtaaca ttctgccagc
    38101 ggctttgttg aataaatcag gttcaagata atcgtctctg catcaggtta gagtttcagg
    38161 caatacatcc tctttctggc atacctgcca ctctagcacc atggtcatag cctttacgat
    38221 ctgcatactc accagaccag agcccgctcc cttttaggct gaggaatccc cagaaaagaa
    38281 gacaggcata gagttttgtg atctactgat tgcgctaaca aattcaatga gatcacctct
    38341 atgcctgccc gtaaagtatg ccagactttt ttccgtaatg ctttagcttc cacgcatcag
    38401 tatcgacaaa atgccattat tgattctgct gctgctttag tcggtggcgc atctctttct
    38461 cttaccagca ttggccggca tttacccggt cctgctcgcg tgaaagataa aataaaacgc
    38521 gttgaccgtc tgttgggtaa taaacgactt cataatgata tccctctgat attcaaaaat
    38581 attacatcca tgatgacaaa taaactgtcg tggtgtgtta tcgctgttga ctggagcggt
    38641 tacccttttc aggaatatca tgtccttcgt gccagcctcc tctgcgacgg tcgctctatc
    38701 cccctgatga gtcaggtctt cccgtcaaaa aaaaataacg aggcggtgga aatcgctttt
    38761 ctggatgcgc tttccggggc catttctccc cggactcgtg ttgttattgt tactgatgct
    38821 ggttttcaaa gtgcatggtt ccgtcatatc aaatcacagg gatgggattt tattggtcgt
    38881 atcagggggg tagtgaagtt caggctggat agtgacaaag ataagtggct ggacataaaa
    38941 atgtgcaggg ggagttcaga ggcaaaatac ctggagacgg gaaccctggc ccggaaaaaa
    39001 cgctcgcagt gtgaaggaca tttttatctt tataaacatt cacctaaagg cagaaaaagc
    39061 aggcgtgcca gagggaggcc gggcttacca acaacggaaa aagaacaaaa agccgcaggc
    39121 agagaaccat ggttaatatt cagcaataca gctgagttta atgccaaaaa aatcatgaaa
    39181 ctgtacagtc gaagaatgca gatagagcaa aactttcggg atgaaaaaag tgaacgcttt
    39241 ggttttggcc ttcgggcgag cagaagtcgg aggggagagc gttttctggt attaagtcta
    39301 ctggttacac tggccagtat cgtactctgg ctacttggtt atcattttga aaataaagga
    39361 tttcatttaa agtatcaggc taatagtctc aaaaaacgaa gagtattgtc ttttctgacg
    39421 ctggctgaaa acgtcctgag atttaaccct gaacttctac gacgagcaca acctgaaaag
    39481 atattgtgcc ggttggccag cacttaccga agtatggtgc tggcttactg atgaaaattt
    39541 tctggggatc cctcagcctt tcggggagga atgagggctc tgatattctt ttgccgtagt
    39601 tcatcgtgaa accgtcggat gtcatttggc ctgagtgacc catgcctccc aaaagcagag
    39661 tcgctagtgt tatgtaatta ggcggttaaa cttcaattct cagtgttaag tttgggtaaa
    39721 cgcttgtaaa tggtattgct aattttttaa ttgactaata ttagaaacgg ttagtgtgat
    39781 tgtatataga tatttgagtg taaggtattt aaaggggcat tcagcccctc cgtctatttg
    39841 tgattgcatc cattgatgtg tgtattattt tgagcttgaa aaagggaggc gctctttaat
    39901 aataaataaa tctatcaaag ctaaatatta aatggaagcc accctttttt ctctgatagt
    39961 tttattatca ccaaaccatt tatttcagct atcgaatctg gattcggcca ggaaggtaaa
    40021 ctcttcacaa ttggataaat cttctctatt tgttctttat ttgctggttt gcaattacag
    40081 attccatagt aattcataaa tgcaaccatc ctaaaatagt tgccaccatc ccaccaaaaa
    40141 aaagattttc caaaagtgtc tgcgctcatg gagagcatat ttcctgagtc gtttgaacca
    40201 tgtatatata tatattttcc gttaagggaa atgccttttg tttgagatgt gtatgatatt
    40261 tctttagcta aagagatatc tttctgtctt gcgagagtat cattcaaaaa tagatttttg
    40321 gatgttgaaa cgccattaaa tataataaat aaaaaaaaca tgcagtttag gtattttata
    40381 gagcgaaaat tgcttaagca agaaaacaaa attactgcta ctataggcat taaaacaaac
    40441 aggcgaggtg gcgcccctga gccaacaaca acgataaata taaccggaag tatataaatg
    40501 aaaatgattg atgaaatcaa ataaattgat cttgttttta atttatatgt tagggtaaat
    40561 atcagaatta ataaaggaat cagtaaccac ttgtataggt ttaaaccagt gggaggattg
    40621 ttatagaagt tatatatatt atctatggct gatttaacaa gccatttaat atcgcttgca
    40681 ttttgtatat aatttgagat gtactcgttc gattcaagcg aaaaataatg cttgatagct
    40741 ttggttaata gcaggtaaat taaggtagat aaagctatga gggatagaga taaacatgaa
    40801 ctataaaaag attcacgaat atttttctca tttcgtatta tcgaatttat ctgcaaccca
    40861 atgacgaatg caataatata tgttacgaat gtttggtaga ttgccattga aagaatggac
    40921 agtactatac cagaaaatat cacaatccta tttttttgcg agtgaaaaat aattgctgat
    40981 atcgctgcca gtagaaaagc aattcccaca gtatcagctt ggttaagaaa ctctaattga
    41041 taggagatct gagggaaggt aataaatact aacataccta tcaataattc ataagattct
    41101 agctttagcg atctgcagat tataaatgct gaaatgatga taaaagataa ggctattaat
    41161 ggtgttatat aaagtgaaaa aggctcaggg agaaagtaat gtcgcaaaaa agtgtgaaac
    41221 caccgcccaa gcgtgattgt ttgataaaaa ttatttgtaa actcgccatc aatattaagt
    41281 gttccatcgt acaaccaccc ccaataacaa taaattgata ttgcaaaaca tgatatgaag
    41341 gcaaggagat ttgcattttt aaataaatta attttaatca tgggttacct cgatgagatt
    41401 tttttatgat gtatttgggt ctatttttta cttcaatata gattctacct atatattctc
    41461 cgagaacacc aatcccgatc agttgcactc cacccaagaa aagtattgat acaagcaggg
    41521 agggataccc gcgtactggg ttaccaaaga caagggtgtc tataatcatc catgcaccat
    41581 ataaaaatga aatgcttgca acaaacaagc ctatataagt ccatacgcga agaggaaagg
    41641 ttgaaaaact tgtgatacct tccagtgcca gattccataa tttccagcca ttaaattttg
    41701 agatgccagc aacacgctct gcgcgtacat attcaacgac atccgtctga ccacccaccc
    41761 agctcagtat gcctttcatg aaaagattgc gctcaggcaa cagtttaatg ttctccacaa
    41821 cctcacgaga catgagtcga aaatctccga cattttcctc gatctttggg gtgcttattt
    41881 tgttgtgtaa tttatagaac cactcagctg tcttacgttt cagtcgtcca tcagttgagc
    41941 ggtctgaacg tttagcaagc accatgtcag cacctgcctg ccacttttca ataagatgag
    42001 ggataacctc aattgggtct tgcaggtcga catcaatagg aattacagca tcgccgcttg
    42061 catggtctaa ccctgcaaat aaggctggtt ctttaccaaa gttgcgtgta aatgacagcg
    42121 gaacaactag cgggtctgaa acagccagcg cgttaataat tgactctgtg gcatctttac
    42181 ttccgtcatt tatgaataca atttctactt catatggctt caactcttgg aattcacgta
    42241 ccgttttata gaaaacaggt atcgcttctt cttcattgaa gacaggaacg acaagagata
    42301 ttttcatttc gcatccctaa agacaatgaa ctttgaatag acgaaaccgc acaccagact
    42361 gatggcggag aaggtgataa gagtgactat tggaggaagt gagcatttat cagctgccca
    42421 accaacaatc acactcaata ttcccataaa tcccacgtat aacatgtagc gcatcgctgt
    42481 agtcgatgct ttgaatgtga atcttgcatt cgcgaagaag ctaaagctca cagccactac
    42541 gaaacctgtg aagtttgcca gagcctgact ggtatgtgcg gcatagatac atacaccaaa
    42601 aaccacccag tgtataagtg tgttcagcac acctatagat gtgtacttta caaataactt
    42661 taacatttat ttaatcaatg agctctgaaa ggcatgaagt ctatcatcca agtctcaatt
    42721 gatcgatact tgctatgtct gatgagacaa aactgagaca cataaggcct cacaatggct
    42781 tgcaaggctt tacatgtttt gatgtggtgg gacgtgtgag cgcagtgttg atgggataat
    42841 cctttgaatt acaagcggat tcttataatt cgtaatgcga aggtcgtagg ttcgactcct
    42901 attatcggca ccatttaaat caataagtta cctcgcattt aagtaaacca cgttctcctc
    42961 ttgtgccgta tttgtgccat tgcgacttat aatcgcatcg attttgcttg cgtgctcggt
    43021 gagatgcccg gctgaaaggt gggcgtatct ttgaaccatt tcgagagttt cccatcctcc
    43081 catctcttta agtgcaagaa gagagacacc ggactgaacc agccagcttg cccaggtatg
    43141 cctcagatca tggaagcgga agttgctaat gcctgcccgc tttaacgctc ccttccatgc
    43201 cttgttgctg tcggttctca tcttccttac cgctgctgtt tttgttccgt cgcttcggta
    43261 ggcaggtttg gtgtggacaa atacccatct cttatggagc ccctgctgtt ttcttaatat
    43321 ctggcatgcg gtttcgttaa gaggaactcc gatcgcattg ccagcttttg tttcatcggg
    43381 gtgcatccat gccattttct tatccagatc gacctgtgac cactc
//
LOCUS       DAOFMX010000028        42886 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.028, whole genome shotgun sequence.
ACCESSION   DAOFMX010000028 DAOFMX010000000
VERSION     DAOFMX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.028"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            4..942
                     /gene="psuG"
                     /locus_tag="QBA20_001718"
     CDS             4..942
                     /gene="psuG"
                     /locus_tag="QBA20_001718"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDQ6292291.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            1036..2286
                     /locus_tag="QBA20_001719"
     CDS             1036..2286
                     /locus_tag="QBA20_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDQ6292292.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2357..3051)
                     /gene="yeiL"
                     /locus_tag="QBA20_001720"
                     /pseudo
     CDS             complement(2357..3051)
                     /gene="yeiL"
                     /locus_tag="QBA20_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            3182..4123
                     /gene="rihB"
                     /locus_tag="QBA20_001721"
     CDS             3182..4123
                     /gene="rihB"
                     /locus_tag="QBA20_001721"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDQ6292293.1"
                     /translation="MEKRKIILDCELGHDDAIAMMMAAKHSAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            4223..5473
                     /locus_tag="QBA20_001722"
     CDS             4223..5473
                     /locus_tag="QBA20_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDQ6292294.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTAQVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIARLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(5529..6617)
                     /locus_tag="QBA20_001723"
     CDS             complement(5529..6617)
                     /locus_tag="QBA20_001723"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDQ6292295.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(6620..7477)
                     /gene="nfo"
                     /locus_tag="QBA20_001724"
     CDS             complement(6620..7477)
                     /gene="nfo"
                     /locus_tag="QBA20_001724"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDQ6292296.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(7551..8600)
                     /locus_tag="QBA20_001725"
     CDS             complement(7551..8600)
                     /locus_tag="QBA20_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDQ6292297.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            8699..9580
                     /locus_tag="QBA20_001726"
     CDS             8699..9580
                     /locus_tag="QBA20_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6292298.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            9786..11255
                     /gene="lysP"
                     /locus_tag="QBA20_001727"
     CDS             9786..11255
                     /gene="lysP"
                     /locus_tag="QBA20_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDQ6292299.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            11547..13538
                     /gene="cirA"
                     /locus_tag="QBA20_001728"
     CDS             11547..13538
                     /gene="cirA"
                     /locus_tag="QBA20_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HDQ6292300.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(13570..14405)
                     /gene="yeiG"
                     /locus_tag="QBA20_001729"
                     /pseudo
     CDS             complement(13570..14405)
                     /gene="yeiG"
                     /locus_tag="QBA20_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            14663..15331
                     /gene="folE"
                     /locus_tag="QBA20_001730"
     CDS             14663..15331
                     /gene="folE"
                     /locus_tag="QBA20_001730"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDQ6292301.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            15348..16424
                     /locus_tag="QBA20_001731"
     CDS             15348..16424
                     /locus_tag="QBA20_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDQ6292302.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            16566..17606
                     /gene="galS"
                     /locus_tag="QBA20_001732"
     CDS             16566..17606
                     /gene="galS"
                     /locus_tag="QBA20_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDQ6292303.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            17886..18884
                     /gene="mglB"
                     /locus_tag="QBA20_001733"
     CDS             17886..18884
                     /gene="mglB"
                     /locus_tag="QBA20_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDQ6292304.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            18945..20465
                     /gene="mglA"
                     /locus_tag="QBA20_001734"
     CDS             18945..20465
                     /gene="mglA"
                     /locus_tag="QBA20_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDQ6292305.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            20481..21491
                     /gene="mglC"
                     /locus_tag="QBA20_001735"
     CDS             20481..21491
                     /gene="mglC"
                     /locus_tag="QBA20_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDQ6292306.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(21527..22739)
                     /gene="preA"
                     /locus_tag="QBA20_001736"
                     /pseudo
     CDS             complement(21527..22739)
                     /gene="preA"
                     /locus_tag="QBA20_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(22733..23971)
                     /gene="preT"
                     /locus_tag="QBA20_001737"
     CDS             complement(22733..23971)
                     /gene="preT"
                     /locus_tag="QBA20_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDQ6292307.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGGKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(24165..24404)
                     /locus_tag="QBA20_001738"
     CDS             complement(24165..24404)
                     /locus_tag="QBA20_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDQ6292308.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(24407..25126)
                     /gene="sanA"
                     /locus_tag="QBA20_001739"
     CDS             complement(24407..25126)
                     /gene="sanA"
                     /locus_tag="QBA20_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDQ6292309.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(25276..26160)
                     /gene="cdd"
                     /locus_tag="QBA20_001740"
     CDS             complement(25276..26160)
                     /gene="cdd"
                     /locus_tag="QBA20_001740"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDQ6292310.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(26290..26985)
                     /locus_tag="QBA20_001741"
     CDS             complement(26290..26985)
                     /locus_tag="QBA20_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDQ6292311.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(26982..27380)
                     /locus_tag="QBA20_001742"
     CDS             complement(26982..27380)
                     /locus_tag="QBA20_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDQ6292312.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            27619..28566
                     /gene="dusC"
                     /locus_tag="QBA20_001743"
     CDS             27619..28566
                     /gene="dusC"
                     /locus_tag="QBA20_001743"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDQ6292313.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(28937..29020)
                     /gene="yohP"
                     /locus_tag="QBA20_001744"
     CDS             complement(28937..29020)
                     /gene="yohP"
                     /locus_tag="QBA20_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDQ6292314.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            29244..30680
                     /gene="mdtQ"
                     /locus_tag="QBA20_001745"
     CDS             29244..30680
                     /gene="mdtQ"
                     /locus_tag="QBA20_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDQ6292315.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            30733..31494
                     /locus_tag="QBA20_001746"
                     /pseudo
     CDS             30733..31494
                     /locus_tag="QBA20_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(31611..32189)
                     /locus_tag="QBA20_001747"
     CDS             complement(31611..32189)
                     /locus_tag="QBA20_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ6292316.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            32359..32946
                     /locus_tag="QBA20_001748"
     CDS             32359..32946
                     /locus_tag="QBA20_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDQ6292317.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            33120..34052
                     /gene="pbpG"
                     /locus_tag="QBA20_001749"
     CDS             33120..34052
                     /gene="pbpG"
                     /locus_tag="QBA20_001749"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDQ6292318.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(34090..35805)
                     /gene="dld"
                     /locus_tag="QBA20_001750"
     CDS             complement(34090..35805)
                     /gene="dld"
                     /locus_tag="QBA20_001750"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDQ6292319.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            36001..38298
                     /gene="bglX"
                     /locus_tag="QBA20_001751"
     CDS             36001..38298
                     /gene="bglX"
                     /locus_tag="QBA20_001751"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDQ6292320.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            38550..39467
                     /gene="osmF"
                     /locus_tag="QBA20_001752"
     CDS             38550..39467
                     /gene="osmF"
                     /locus_tag="QBA20_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDQ6292321.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            39474..40631
                     /gene="yehY"
                     /locus_tag="QBA20_001753"
     CDS             39474..40631
                     /gene="yehY"
                     /locus_tag="QBA20_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDQ6292322.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            40624..41550
                     /gene="yehX"
                     /locus_tag="QBA20_001754"
     CDS             40624..41550
                     /gene="yehX"
                     /locus_tag="QBA20_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDQ6292323.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            41555..42286
                     /gene="yehW"
                     /locus_tag="QBA20_001755"
     CDS             41555..42286
                     /gene="yehW"
                     /locus_tag="QBA20_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDQ6292324.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGVGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(42267..42374)
                     /locus_tag="QBA20_001756"
     CDS             complement(42267..42374)
                     /locus_tag="QBA20_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDQ6292325.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(42434..42886)
                     /locus_tag="QBA20_001757"
                     /pseudo
     CDS             complement(42434..42886)
                     /locus_tag="QBA20_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        10306 a        10609 c        11457 g        10514 t
ORIGIN      
        1 agaatgtctg aattaaaaat ctcccctgaa ttattacaaa tttctccgga agtgcaggac
       61 gctttaaaaa acaaaaaacc ggttgtggcg ctggaatcga ccattatttc tcacgggatg
      121 ccgttcccac aaaatgccca gaccgcaatt gaagtagaag aaactattcg taaacagggc
      181 gcagtacctg ccactatcgc cattattggc ggcgtgatga aagtgggttt aagcaaagaa
      241 aaaattgaat tactgggtcg tgaagggcat aacgtgacca aagttagtcg tcgcgattta
      301 ccttttgttg ttgccgccgg aaaaaatggc gcaaccactg tggcttcaac gatgattatt
      361 gcggcgcttg ccggaattaa agtatttgcc accgggggaa ttggtggtgt gcatcgcggg
      421 gcggaacata ccttcgatat ttctgccgat ttgcaagaac tggcaaatac taatgtcacc
      481 gttgtttgtg ccggggcgaa atctattctc gatttaggat taaccactga gtatttagaa
      541 accttcggtg tgccgttaat tggctatcag actaaagcgc tgcctgcgtt tttctgccgt
      601 accagctcgt ttgacgtcag cattcgtctc gacagcgcca gtgaaattgc ccgtgcaatg
      661 gcggtgaaat ggcaaagcgg gctgaacggt ggcctcgtgg tagcgaaccc gatcccggaa
      721 cagtttgcga tgccggagga atctatcaat gcagccatag atcaagccgt cgccgaagcc
      781 gaagagcagg gcgttattgg taaagaaagt acaccgttcc tgctggcgcg cgttgctgag
      841 ctgaccggcg gtgacagcct gaaatccaat atccagctgg tgttcaacaa cgccattctg
      901 gcgagtgaaa ttgccaaaga ataccagcgt ctcgcgggtt aatttctgac gggcagaaag
      961 ccgtcgcccg tactcatcaa gcataccaaa acctggcgct gacgccgggt ttactggcat
     1021 gaagggaaaa tcattatgga tataatgaga agtgttgtgg ggatggtggt gttactggca
     1081 atagcatttc tgttgtcagt gaataaaaag agcatcagtt tgcgcacggt tggagccgca
     1141 ctgctgctgc aaatcgctat tggtggcatc atgctctact tcccaccggg aaaatgggca
     1201 gtagaacagg cggcattagg cgttcataaa gtgatgtctt acagtgatgc cggtagcgcc
     1261 ttcatttttg gttcgctggt tgggccgaaa atggatgtcc tgtttgacgg tgcgggtttt
     1321 atcttcgcct ttcgcgtact tccggcgatt attttcgtta ctgcgctcat cagtctgctg
     1381 tactacattg gcgtgatggg gctgctgatt cgcatccttg gcagcatttt ccagaaagcc
     1441 ctcaacatca gcaaaatcga atcttttgtt gcggttacca ctattttcct cgggcaaaat
     1501 gagatcccgg cgatcgttaa accgtttatc gatcgcgtga atcgcaacga gttgtttacc
     1561 gcaatttgta gcgggatggc gtccattgct ggttcggtga tgattggtta tgccggaatg
     1621 ggcgtaccaa ttgactacct gttagcggca tcgctgatgg cgatccccgg cggtattttg
     1681 tttgcacgta ttcttagccc ggcaaccgag ccttcgcagg tcacatttga aaatctgtcg
     1741 ttcagcgaaa cgccgccaaa aagctttatc gaagcggcgg cgagcggtgc gatgaccggg
     1801 ctgaaaatcg ccgctggtgt agcgacggtg gtaatggcgt ttgtcgcaat tattgcgctg
     1861 atcaacggca ttatcggcgg aattggcggc tggtttggtt tcgccaatgc ctctctggaa
     1921 agtatttttg gctatgtgct ggcaccgctg gcgtggatta tgggtgtgga ctggagtgat
     1981 gccaaccttg caggtagcct gattgggcag aaactggcga ttaacgaatt cgtcgcttac
     2041 ctgagtttct ccccatacct gcaaacgggc ggcacgctgg aagtgaaaac cgtggcgatt
     2101 atatcctttg cgctttgtgg ttttgctaac tttggttcta tcggtgttgt cgttggcgca
     2161 ttttcggcta tttcgccaaa acgtgctcca gaaatcgccc agcttggttt acgggcgctg
     2221 gcagcagcaa cgctttccaa cctgatgagt gcgactattg ccgggttctt tattggtctg
     2281 gcgtaatgct cgatttgtag accggataaa acgtattaag cgttgcatcc ggcaatcgcg
     2341 ctattgcaaa taatttttac tgcatcatcc cggagaattt attctccggg tccatctcca
     2401 gcgccagtcc tgacaactgc tttctgtttt taattagata tcctttcttt taattaataa
     2461 accgtcgtga atgaattgcg cgagaacata taaaagatgt cgataagaaa cgcctaaata
     2521 ctctgccgct tgcgtatgct tttcgtgata aagatcacct tcttgtgaga gtaatataaa
     2581 tgctgccagg cgattcacta acggaaatga ttgattctga gttaaagaaa caatattacg
     2641 ataattttta tgacttaagg tgacgcaaag ttttcgtaaa aatagggtgt cgtttaataa
     2701 cagcggacgg taatgtttca tagggagcgc aaggcaccaa cactcttcaa tagcctgcac
     2761 cgcacgcggt tcatggtctt tgtcgattaa ctcaatctcg ccaataaaac agggagcggc
     2821 aaagaaatcg atcagcgaca cgcgaccatt ggcaagcgtg gcgtaaagcc tggcgcggcc
     2881 tcgcgtcaga taaaacagcc atgacggctg ttgcccttcc tgcacaatgt agtcacgcgc
     2941 taaaaaatga aacagccgcg tatcggctga aacatccgtt gaaaagcaat ccttaaacgc
     3001 agattcactc atcaattgct gcttaaggtc attattgtgg atttctttca cgatgtttat
     3061 ccgctatgag atttctcata tttagcatac gtacgctgtt gttaagattc aatcattggc
     3121 ccggtcgaat tttctttgct gtagaaaaaa ataacgcaac tggaaacaga ggaaataaac
     3181 aatggaaaag agaaaaatta ttctggattg tgagctgggt catgatgatg ctattgcaat
     3241 gatgatggca gcgaaacatt cggcaataga tttattaggc atcactattg tagcgggtaa
     3301 tcagacgctt gataaaacat taattaatgg cctgaatgtt tgccagaaac tggagattaa
     3361 tgttccggtt tatgcgggga tgccgcaacc cattatgcgt aaacaaatcg ttgccgataa
     3421 tattcacggt gaaaccggac tggatggccc ggtattcgag ccgctgaccc gccaggcaga
     3481 aagtacccat gcggtgaaat atatcatcga taccctgatg gcaagcgatg gcgatatcac
     3541 tctggtgccg gttggtccgc tttcaaatat cgcggtggca atgcgtatgc aacccgcaat
     3601 cctgcccaaa atccgtgaaa ttgtgctaat gggcggcgct tacggtacag gcaacttcac
     3661 cccatctgcc gagttcaata tctttgccga cccggaagcc gcccgcgtgg tgttcacctc
     3721 cggcgttcca ttagtgatga tgggcctcga tcttaccaac cagaccgttt gcaccccgga
     3781 tgtaattgct cggatggaaa gggtaggcgg ccctgccgga gagctgttca gcgacatcat
     3841 gaacttcact ctcaaaacgc agttcgaata ctacggcctg gctggcggcc cggtgcatga
     3901 cgccacctgc attggttatc tgatcaaccc tgatggcatt aaaacccagg atatgtacgt
     3961 cgaagtggac gtcaacagcg gcccttgcta cgggcgtacg gtctgcgacg aactgggcgt
     4021 tcttggcaag cccgctaata ccaaagtcgg catcactatt gataccgact ggttctgggg
     4081 cttagtcgaa gagtgcgtgc gcggctacat caaaacccat taagtgttga taacaagccg
     4141 ggacagtatc gcaagatgca ggcccggcta tttctgatgg gcagaaagcc gtcgcccaaa
     4201 atgacatgaa gagataatca ctatggatgt catgagaagt gttctgggaa tggtggtact
     4261 gctggcaatt gcgtttttac tgtcagtaaa caagaagaag atcagcctgc gtaccgttgg
     4321 cgcggcgtta gtgttacagg tcgtgattgg cgggattatg ctttggttac cgccagggcg
     4381 ttgggtcgca gaaaaagtcg cttttggcgt ccacaaagtg atggcgtaca gcgacgcagg
     4441 tagcgcattt atcttcggtt ctctggtcgg accgaaaatg gataccttat ttgatggcgc
     4501 aggatttatc tttggtttca gggtattacc ggcaattatc ttcgtcactg cacaggtgag
     4561 tattctctac tacatcggtg tgatggggat cttaattcgc attctcggcg gtatcttcca
     4621 gaaagcttta aatatcagca agatcgagtc attcgtcgcg gtcaccacca ttttcctcgg
     4681 gcaaaacgaa attccggcga tcgtcaaacc ctttatcgat cgtctgaatc gcaatgaatt
     4741 atttacagcg atttgtagtg gcatggcctc gattgctggt tcgacaatga ttggttacgc
     4801 cgcactgggc gtacctgtgg aatatttgct ggcggcatcg ttaatggcga tccctggcgg
     4861 gatcttgttt gcccgcctgt taagcccggc tacggaatct tcgcaggttt cttttaataa
     4921 cctctctttc accgaaacac cgccaaaaag cattattgaa gccgatgcga caggggcaat
     4981 gaccgggctg aaaatcgccg ccggtgtagc gacagttgtt atggcatttg tcgccatcat
     5041 tgcgttaatt aacggtatta tcggcggcgt tggcggctgg tttggttttg aacatgcctc
     5101 gctggagtcc attttaggtt acctgctggc cccacttgcg tgggtaatgg gtgttgactg
     5161 gagtgatgca aatcttgccg ggagtttgat tggacagaaa ctggcaataa atgaatttgt
     5221 cgcttatctc aatttctcac cctatctgca aacggctggc actctggatg ctaaaaccgt
     5281 ggcgattatt tccttcgcgt tgtgcggttt cgctaacttt ggttctatcg gggtggtggt
     5341 gggggcgttt tctgcggttg cgccacaccg tgcgccggaa atcgcccggc ttggtttacg
     5401 cgcgctggcg gcggcgacac tttctaatct gatgagtgcc accattgccg ggttctttat
     5461 tggtttagcg tagtgtgcaa tttatgagcc ggattagatg cggtcaagcg tcgaatccgg
     5521 caacattgtt acaccattgg cactaatgac agcgcgttat cggcagacag ggtagggttg
     5581 tttaagctgc cgctggcgcg tgaaattgcc gcgcaggcca tcgcaaaacg ggcgctgtca
     5641 cggaaattgt ttccctccag aaagctatat accagacccg ccataaaacc atcgtcagca
     5701 ccaaaactgt ctaccgtcgt atgcgctggc acagtcagca aaaattgttc accatccttt
     5761 tcgctgcaat aaactgactc atcgggcaaa taaacaaaca gttgctgaac accttgctga
     5821 tgcaatgcat tcactgcggc attacggtca gcatcgctgg tgatcgcctg tccccataaa
     5881 atttccagct ccggtaaagt gggtttcagg gtgtgaatat gcgccagcca gtgtttgatt
     5941 ttgcccgctt tgaattctga aacggtatcg acaaacaccg ggatctcatc agcgagggta
     6001 aagacccatt ccagcgcctc ggctgtcagg ttgcaatcag ccagtaccac gcccgcatga
     6061 cgaagtaaat cgcgcgaccc gttcagtagt tgcggtgtca actgttccag cagatgggta
     6121 tcgttaattg ccagcacggt ttgatcgtct cgattggcaa ttgccagata cgtcgatgtg
     6181 ctttgaccat gcaaacgaac gcagccggag acattcacgc cggcacggcg cgtttcttcc
     6241 aggagcattt cgccataaaa gtcatcgcca atcactgaaa gcaaatggac gtcacggcct
     6301 aacagcgcca gattgtgggc gatattgcgt ccaacgccgc ctgccgaaca atgaattgta
     6361 ccgggatgag aagccgcttg cgggtaatgg atatccgcca tcccgcgaat atccatattg
     6421 attgtcccca ccactacgca gtattcctgc tcggtgagaa tgtaaccttt gtctttaatc
     6481 cggcctttgc gcattaaatc cataatatgc gccgcaacgc gcgaacgact gatttgcagc
     6541 atgtccgcaa tttcgttctg ctgaatcagc gggttacgcc gtaaaattgc aaggatctcc
     6601 ttttcccggt tattcatctt caggctaccg ctttttcagt ttgttgcgct ttcagccagg
     6661 cgatctcttc tgcccagata tccgggttga tggtttcaag gatcagcgga atgccgtcga
     6721 aacggtcgtt ctgcataatc cagcggaacg catcatgacc gatattgcct tcaccgaggc
     6781 tatgatggcg gtcaacgcgg ctgccaaagg tacttttcgc atcgttaagg tgcatcccgc
     6841 gcagatactt aaagccgaca atacgggcaa aatcagcgaa cgttttttcg cattcggctg
     6901 gagtacgcaa atcatacccg gcagcgaaag catggcaggt atcaatgcag acgccgacgc
     6961 gggatttatc ttccacgccg tcgataatcg ccgcgagatg ttcgaattta aaccctaagt
     7021 tactgccctg accggcggtg ttttcaatca ccgctgttac accttgagtt ttatccagcg
     7081 caatgttgat ggattcggca atacgcgcaa ggcaatcctc ttctgaaatc tgcatcagat
     7141 ggctgccagg gtggaagttg agcaaagaaa gccccagttg ttcgcaacgc tgcatttcat
     7201 ctataaaggc atcgcgcgat ttttccagag cttcagtgac cggatgtccg aggttgatca
     7261 gataactgtc gtggggaaga atttgcgccg atgtgtagtg atatttttca caggcggctt
     7321 tgaattcatc gatggtttgc gtcgtgagcg gtgcggcacg ccactgacgt tggtttttgg
     7381 tgaacaaggc aaacgcggtt gcgtcgattt cggcggcgcg aattgcggca tttgccagac
     7441 cgccagcagc actaacgtgc gctccaatgt atttcatgcg aggactcctg ttaaacccgc
     7501 tggaggaaaa cggtaatgat agcgggttaa caggagcaag aagtagtggt ttatgcgatg
     7561 acgctttgaa tcacatagtt aatcgcacca ccaccaacaa tcagccaggc aaacagtacc
     7621 agtgccatca gcagaggttt cgccccagct tttttcagcg cgctgacgtg agtggtcaga
     7681 cccagcgccg ccatcgccat tgccagcagg aaggtatcca gcgttaccag catgttcacc
     7741 acgctctgcg gtaacaggtg gaacgagtta aagatggcaa ctacgatgaa caagatggca
     7801 aaccacggaa tagtgatttt gcttttctcg ccgctgttcg ccccagacag ctgtttaaca
     7861 cgcgccgcca gcaggatgag gaacggagcc agcatcatca cgcgcagcat tttggaaata
     7921 actgctgcgt tttctgcatc cgggctgatg gcatgacctg ccgccaccac ctgcgccact
     7981 tcgtgcacag tagaaccaat gtagataccg aaagtttccg gactaaacca ttgagacatc
     8041 agcggatata tcgccgggta gaggaaaatc gcgacggtcc cgaagataac aacggttgca
     8101 acagccacgg ttactttact ggcttccgct ttcactaccg gctcagtcgc cagtaccgcg
     8161 gcagcaccac agatactgct accggcaccg atcaaccaac tggtgtgctt atccagacca
     8221 aacactttct gccccaggaa gcaagccagc aggaaggtac tggacagcgt cagcacgtca
     8281 atgatgatcc cactgatacc gacatcggca atttgcgaga acgtcagacg gaagccataa
     8341 agaatgatac ccagacgtaa taaatattgc ttggcaaaca gcacaccacc gtcacagctt
     8401 ttccagatgt gcggatagat ggtgttgcct aaaaccatcc ccaacaagat tgcgagggtg
     8461 agggcactaa acccggcacc cgcaaccgcg ggaatggaac caccccacag ggcgaccccg
     8521 gtgataactg cactcagggc taaccccgga ataaaatgcc acagtgtacg atgttgtttc
     8581 tgtaaggtga tattcgtcat aaccctctcc tttaaccggg cataaggtta cgactaactg
     8641 gtttaaaaat aaaattgatt atatatttat aattaatctt tataagtggt aagcgactat
     8701 gcacatcacc ctccggcagt tggaagtttt tgcagaagta ttgaaaagtg gatcaaccac
     8761 ccaggcgtcg gtgatgctgg cgttgtcgca atcagcagtg agcgcagcct tgactgacct
     8821 ggaagggcag cttggcgttc aactgtttga tcgcgtgggg aaaagactgg ttgttaatga
     8881 acacgggcgg ctgctctatc cgcgagcgtt ggcactgctt gaacaggcgg ttgaaatcga
     8941 acaactgttt cgcgaagaca acggcgcgat tcgtatctat gccagtagta ccatcggtaa
     9001 ctacattctg cctgcagtta tcgcccgtta tcgccatgat tatccgcagt tgccgattga
     9061 acttagcgtt gggaacagcc aggacgtgat gcaagcggtg ctggatttcc gcgttgatat
     9121 tggctttatt gaaggaccgt gccacagcac tgaaatcatt tctgaaccgt ggctggaaga
     9181 cgagctggtg gttttcgccg cgccgacttc gccgttggct cgtggtccgg tcaccttaga
     9241 acagctggcc gctgcgccgt ggatcctgcg tgaacgcggt tccggcacgc gggatattgt
     9301 cgattatctg ttgctgtcac atttaccgaa gtttgagatg gcgatggaat taggtaactc
     9361 cgaggcaatc aaacatgcgg tgcgtcatgg gttgggaatt agttgcctgt cgcgacgtgt
     9421 gattgaagat caattgcagg caggcacatt aagtgaagtt gcggtccctc tgccgcgcct
     9481 gatgcgtacg ttgtggcgta tacatcatcg gcaaaaacac ctttccaacg cgctacggcg
     9541 ctttctggac tattgcgatc ccgcaaatgt gccgcgttaa gttgctgtac aagaacatgc
     9601 tggtgctgtg tcgatttcgt gacgcagcgc cttcagcatg cattcgccag aaaagagatt
     9661 ggctgcttta cttataatcc ctgggcgatc atgaaggtgt cttataaccg tgtatttctg
     9721 ccggaaggat tgccaatcgt ctgctacaat cgcgccatca tttttaagat ggatagcatt
     9781 tttgtatggg ttccgaaact aaaactacag aagcgccggg cttacgccgt gaattaaagg
     9841 cgcgtcacct gacgatgatt gccattggcg gttccatcgg tacaggtctt tttgttgcct
     9901 ctggcgcaac gatttctcag gcaggtccgg gcggggcatt gctctcgtat atgctgattg
     9961 gcctgatggt ttacttcctg atgaccagtc tcggtgaatt ggctgcatat atgccggttt
    10021 ccggttcgtt tgccacttac ggtcagaact atgttgaaga aggctttggc ttcgcgctgg
    10081 gctggaacta ctggtacaac tgggcggtga ctattgccgt tgacctggtt gcagcacagc
    10141 tggtcatgag ctggtggttc ccggatacac cgggctggat ctggagtgcg ttgttcctcg
    10201 gcgttatctt cctgttgaac tacatctctg ttcgcggctt tggtgaagcg gaatactggt
    10261 tctcactgat caaagtcacg acagttattg tctttatcat cgttggcgtg ctgatgatta
    10321 tcggtatctt caaaggcgcg cagcctgcgg gctggagcaa ctggacaatc ggcgaagcgc
    10381 cgtttgctgg tggttttgcg gcgatgatcg gcgtagctat gattgtcggc ttctctttcc
    10441 agggaaccga gctgatcggt attgctgcag gcgagtccga agatccggcg aaaaacattc
    10501 cacgcgcggt acgtcaggta ttctggcgaa tcctgttgtt ctatgtgttc gcgatcctga
    10561 ttatcagcct gattattccg tataccgatc cgagcctgct gcgtaacgat gttaaagaca
    10621 tcagcgtcag tccgttcacc ctggtgttcc agcacgcggg tctgctctct gcggcggcgg
    10681 tgatgaacgc agttattctg acggcggtgc tgtcagcagg taactccggt atgtatgcgt
    10741 ctactcgtat gctgtacacc ctggcgtgtg acggtaaagc gccgcgcatt ttcgctaaat
    10801 tgtcgcgtgg tggcgtgccg cgtaatgcgc tgtatgcgac gacggtgatt gccggtttgt
    10861 gcttcctgac ctccatgttt ggcaaccaga cggtatacct gtggctgctg aacacctccg
    10921 ggatgacggg ttttatcgcc tggctgggga ttgccattag ccactatcgc ttccgtcgcg
    10981 gttacgtatt gcagggacac gacattaacg atctgccgta ccgttcaggt ttcttcccac
    11041 tggggccgat cttcgcattc attctgtgtc tgattatcac tttgggccag aactacgaag
    11101 cgttcctgaa agataccatt gactggggcg gcgtagcggc aacgtatatt ggtatcccgc
    11161 tgttcctgat tatttggttc ggctacaagc tgattaaagg aactcacttc gtacgctaca
    11221 gcgaaatgaa gttcccgcag aacgataaga aataagtttc ctcccttcct tgctaagccc
    11281 tctcaaccga gagggctttt tcaattccat ttccctgaca aatcatgcgg atataaaatt
    11341 taacatttgg attgataatt gttatcgttt gcattatcgt tacgacgcaa tcaaaaaagg
    11401 ctgacaaatc agaggctgtt ccggctttct gggatgatca cctgcataca aataagtcca
    11461 ccgcgatgct gccgtacgca aggggacgta aagaagatgt gagcgatacc cattttattt
    11521 tcgtagttac ctcatggaga tatggaatgt ttaggttgaa ccctttcgta cgggtcgggc
    11581 tgtgtttgtc cgctatttct tgtgcatggc ctgtgttagc ggtcgatgat gatggcgaaa
    11641 cgatggttgt cactgcatct tccgtggaac aaaatcttaa agatgcaccc gccagtatca
    11701 gcgtcattac ccaggaagac ctgcagcgaa aaccggtaca gaatctgaag gatgtcctca
    11761 aagaagtgcc tggcgtacaa ctgacgaacg aaggggataa ccgtaagggc gttagtattc
    11821 gtggtctgga cagcagctac accctgattc ttgtcgacgg taaacgcgtt aactcccgca
    11881 atgccgtctt ccgccacaat gatttcgatc tgaactggat cccggtcgat tccatcgaac
    11941 gtattgaagt ggttcgtggc ccgatgtcgt cgctatacgg ctccgatgcg ctcggcggtg
    12001 tagtgaatat catcaccaaa aaaatcggtc agaaatggtc gggtaccgtt accgtcgata
    12061 ccaccattca ggaacatcgc gatcgcggtg atacctataa cggtcagttc tttaccagtg
    12121 gaccattaat tgatggtgtg ctgggaatga aagcttacgg cagcctggca aaacgtgaaa
    12181 aggatgaccc gcaaaactca acgaccaccg ataccggaga aacgccgcgt attgaaggat
    12241 tctccagccg cgacggcaat gtcgaatttg cctggacacc gaatcaaaat cacgatttta
    12301 ctgccggata cggtttcgac cgtcaggatc gtgattccga ttcgctggac aaaaaccgcc
    12361 tggaacgcca gaactactgc gtcagccata atgggcgttg ggattacggc accagcgaac
    12421 tgaaatccta cggtgagaaa gtcgagaaca aaaaccctgg caacagcagc ccgataactt
    12481 ccgaaagcaa tacggtcgac ggcaaataca cgttgccgct gacggcgatt aatcagtttc
    12541 tcacggttgg cggtgaatgg cgtcacgaca aacttagcga tgcggtgaac ctgaccgggg
    12601 gaaccagctc caaaacgtct gccagccagt acgcgctgtt tgtggaagat gaatggcgga
    12661 tcttcgagcc gctggcgctg acgaccggcg tgcgtatgga cgatcacgaa acctacggtg
    12721 aacactggag tccgcgtgcc tacatggttt ataacgccac cgacaccgta acggtgaaag
    12781 ggggctgggc gacggcattt aaagcacctt ctctgttgca acttagccct gactggacga
    12841 gcaattcctg ccgtggcgca tgtaagattg tgggtagccc ggatctgaaa ccagaaacca
    12901 gcgaaagttg ggagctgggg ctttactaca tgggtgaaga aggctggctg gaagggggtg
    12961 aatccagcgt tatcgttttc cgtaacgatg tgaaagatcg tatcagcatc agccgtacgt
    13021 ctgacgtcaa cgctgcaccg ggctaccaaa actttgttgg ttttgagacg ggcgctaacg
    13081 gacggcgcat accggtattt agctactaca gcgttaacaa agctcgtatt cagggcgtgg
    13141 aaaccgaact gaaaattccg ttcaacgatg aatggaaact gtcgatcaac tacacctaca
    13201 acgatggtcg tgatgtcagc aacggcgaaa acaaaccgct atccgatctg ccgttccata
    13261 ctgctaacgg tacgctggac tggaaaccgc tggcgctgga agactggtca ttctatgttt
    13321 ctggtcacta taccgggcag aaacgcgccg acagcgcgac ggctaaaaca ccgggcggtt
    13381 ataccatctg gaataccggc gcggcctggc aggtgactaa agacgtcaaa ctgcgcgcag
    13441 gcgtgctgaa ccttggcgac aaggatctca gtcgtgacga ctacagctat aacgaagacg
    13501 gacgtcgtta ctttatggca gtggattatc gcttctgatg agaagatgcc cggcgaaccg
    13561 ggcggacttt cacttcagta aatactgcgc atggaagcgc aggtgatcct ctataaaaga
    13621 ggcgatgaag tagtaactgt gatcatatcc cggctgaata cgcagcgtca tcggccacgc
    13681 tttctggcgc gcggcttctg ccaataccgc aggttgcaac tggtcggcaa gaaactgatc
    13741 attatcgccc tgatcgataa gcgtcgggat cgcatcctgc gcgttactgg catacatcag
    13801 cgcgcaacta tcccattcca gcatgcattt ttgtcctcac ctaaataggt gctaaacgct
    13861 ttgattcccc acgggacgct gcacggattc acaatgggcg caaaggccga aacgctggtg
    13921 tatttacccg gatttttcag cgccataatc agcgcaccgt gaccacccat tgagtgaccg
    13981 ctaatggcgc agcggtcgct gacattaaat tgcgactgaa ccagcgccgg taattcatcg
    14041 cgcagataat catacatccg gtaatgcgtc gcccacggcg gttgcgtggc attaagataa
    14101 aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg caaccttttc gccgcgcggg
    14161 ctggtgtctg gcatcaccag tacaatcccc agttccgccg ctacccgctg ggcacccgcc
    14221 ttggtggtga agttctcgtc attgcaggtt aatccggaaa gccagtacag cactggtggc
    14281 ggggtgtgat cacgaggggg tgggagaaag atactgaacg tcatcgggca gtttaaggta
    14341 ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac agcggtgctc ttcgagcatt
    14401 tccatgcgtg gctccttgtt gtgttgtttg caatttcttc atcataccgt ttattcaatg
    14461 tgctgtgagt aactttcact tccgtatttg cataacgatg ttttaacatc tgctgatgaa
    14521 aggcagcggc aattacaata attatcgctg tgaatactgg attatgtgcg ccgcctcacg
    14581 cacaataatc aagctgtaaa tcagcttaat aactttgccc ccacgcaggg cggaggcgtc
    14641 acacctgcag gagaaatcat aaatgccatc actcagtaaa gaagcggccc tggttcatga
    14701 agcgttagtt gcgagaggac tggaaacacc gctgcgcccg cccgtgcatg aaatggataa
    14761 cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa atcatgcagc tgctgaatct
    14821 cgacctggct gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa tgtatgtcga
    14881 tgaaattttc tccggtctgg attacgccaa tttcccgaaa atcaccctca ttgaaaacaa
    14941 aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact ctgaccagta cctgtgaaca
    15001 ccattttgtt accatcgatg gcaaagcgac ggtggcttat atcccgaaag attcggtgat
    15061 cggtctgtca aaaattaacc gtatcgtgca gttctttgcc cagcgtccgc aggtgcagga
    15121 acgtctgacg cagcaaattc ttattgcgct acaaacgctg ctgggcacca ataacgtggc
    15181 tgtctcgatc gacgcggtgc attactgcgt gaaggcgcgt ggcatccgcg atgcaaccag
    15241 tgccacgaca acgacctctc ttggtggatt gttcaaatcc agtcagaata cgcgccacga
    15301 gtttctgcgc gctgtgcgcc atcacaactg attaaaaggc aggaaccatg gagcgcaacg
    15361 tcacgctcga ttttgttcgc ggcgtcgcca ttctggggat cctgctatta aacatcagcg
    15421 cctttgggct accaaaggcg gcttatctca atcccgcctg gtacgatgct attacgccgc
    15481 aggatgcatg gacctgggca tttctcgatc tcatcggcca ggtgaaattc ctcacgcttt
    15541 ttgcgctgct gtttggtgct ggcctgcaaa tgttgctgcc ccgtggcaga ctctggatcc
    15601 agtcgcggtt aacgctgtta gtcttgttgg gctttattca cggtttattg ttctgggacg
    15661 gcgatattct gctggcttac gggctggtgg ggcttggcgt tttgctgtta ttggggctga
    15721 tttccgacag ccagaccagc cgcgcctgga cgccggatgc atcggctatt ttgtatgaaa
    15781 aatactggaa gcttcacggc ggcgttgaag cgatcagtaa tcgtgccgat ggtgttggca
    15841 acagtttact ggcactgggc gcacagtatg gctggcaact ggcagggatg atgctcattg
    15901 gtgcggcgct gatgcgcagc ggctggctga aagggcagtt cagcttacgt cactatcgtc
    15961 gtactggttt tgtgctggtg gcgattgggg tgaccattaa ccttcctgcc atcgccctgc
    16021 aatggcagct ggactgggcg tatcgctggt gtgccttctt acttcaaatg ccgcgggaac
    16081 tgagtgcgcc gtttcaggcg attggctatg cgtcgctgtt ttatggcttc tggccgcaat
    16141 tgagccgctt taagctggtg cttgcgatcg cctgcgtcgg acggatggcg ctgaccaact
    16201 atctattgca aacgctgatt tgtaccacgc ttttttacca cctcggtttg tttatgcatt
    16261 ttgaccgcct ggagctgctg gcgtttgtta ttccggtatg gctggcgaat atcctcttct
    16321 ctgttatctg gctgcgttac ttccgccagg ggccggtgga atggctctgg cgtcagttaa
    16381 ctttgcgtgc tgccggaccg gcaatatcta aaacatcaag ataacgatct ggattacatt
    16441 cgttaacaaa acggctgtaa ccgtttccat tgctgtgact cgattcacga agtcctgtat
    16501 tcagtgctga caaaatagcc gccagcaagc agtcatttac tgcaatctca taacaggtag
    16561 tgaatatgat caccattcgt gatgtagcgc gtcaggctgg cgtctctgtg gcaacggttt
    16621 cccgggtgct caataacagc acgctggtca gtgccgacac gcgtgaagca gtaatgaaag
    16681 ccgtgagtga gctggattat cggccaaacg ccaatgccca ggcgctggca actcaggtta
    16741 gcgacaccat tggcgtggtg gtgatggacg tttctgatgc gtttttcggc gcgctggtaa
    16801 aagcggtgga tctggtcgcc cagcagcatc agaaatacgt gctaatcggc aatagctatc
    16861 atgaagcgga aaaagagcgt cacgccattg aggtgttaat tcgccagcgt tgtaatgcgt
    16921 tgattgttca ctcaaaagca ttgagtgacg atgaactggc gcaatttatg gataacattc
    16981 ccggtatggt gttaatcaac cgcgttgtgc cggggtacgc ccatcgttgc gtttgcctgg
    17041 ataatctcag cggtgcccga atggcgacgc gcatgttgct gaataacggt catcaacgta
    17101 ttggttatct ttcttccagc cacggcattg aagatgacgc catgcgtaaa gcaggctgga
    17161 tgagtgcgtt gaaagagcag gatattattc cgccggaaag ctggattggc actggtacgc
    17221 cggacatgcc gggcggtgag gcggcgatgg ttgaactgct ggggcgcaat ctacaactta
    17281 ccgctgtatt tgcttataac gacaatatgg ccgctggcgc actgacagca ttaaaagata
    17341 atggcattgc gattccgtta catctctcaa tcatcggttt cgatgatatt cccatcgccc
    17401 gttacaccga cccgcaatta acgaccgtgc gttatcccat tgcttcaatg gctaaattag
    17461 ccaccgaact ggccttgcag ggggcagcag gcaatattga tcctcgtgcc agccactgtt
    17521 ttatgccgac gttagtgcgt cgccattctg tcgcaacgcg ccagaatgcg gcggcgatca
    17581 ctaactcaac aaatcaggcg atgtaaccgc tttcaatctg tgagtgattt cacagtatct
    17641 taacaatgtg atagctatga ttgcaccgtt ttaatgttgt aacccgtatg taacagtgaa
    17701 taatcacttt caccgaggta atagcgtcat aacaacaatt aaagccgttt tctggagcgt
    17761 taccgggcat ggaagaacga attttaaaaa gtgagcttcg gcgttcagta acacttcatt
    17821 aactctactg ccccgccgag catttatctc aagcactacc ctgcataaga aaaaccggag
    17881 ataccatgaa taagaaggtg ttaaccctgt ctgctgtgat ggccagcatg ttattcggtg
    17941 ccgctgcaca cgctgctgat actcgcattg gtgtaacaat ctataagtac gacgataact
    18001 ttatgtctgt agtgcgcaag gctattgagc aagatgcgaa agccgcgcca gatgttcagc
    18061 tgctgatgaa tgattctcag aatgaccagt ccaagcagaa cgatcagatc gacgtattgc
    18121 tggcgaaagg ggtgaaggca ctggcaatca acctggttga cccggcagct gcgggtacgg
    18181 tgattgagaa agcgcgtggg caaaacgtgc cggtggtttt cttcaacaaa gaaccgtctc
    18241 gtaaggcgct ggatagctac gacaaagcct actacgttgg cactgactcc aaagagtccg
    18301 gcattattca gggcgatttg attgctaaac actgggcggc gaatcagggt tgggatctga
    18361 acaaagacgg tcagattcag ttcgtactgc tgaaaggtga accgggccat ccggatgcag
    18421 aagcacgtac cacttacgtg attaaagaat tgaacgataa aggcatcaaa actgaacagt
    18481 tacagttaga taccgcaatg tgggacaccg ctcaggcgaa agataagatg gacgcctggc
    18541 tgtctggccc gaacgccaac aaaatcgaag tggttatcgc caacaacgat gcgatggcaa
    18601 tgggcgcggt agaagcactg aaagcacaca acaagtccag cattccggtg tttggcgtcg
    18661 atgcgctgcc agaagcgctg gcgctggtga aatccggtgc actggcgggc acagtactga
    18721 acgatgctaa caaccaggcg aaagcgacct ttgatctggc gaaaaacctg gccgatggca
    18781 aaggtgcggc tgatggcacc aactggaaaa tcgacaacaa agtggtccgc gtaccttatg
    18841 ttggcgtaga taaagacaac ttggctgaat tcagcaagaa ataagactga ttgggcgcag
    18901 tcgtttactg cgcccttatt gtatggccaa caaggtaaaa ttatatggtc agctcaacga
    18961 ctccgtcttc cggggaatac ttgttggaaa tgagcggtat caacaagtcc tttcctggtg
    19021 ttaaggcact tgataacgtt aatttaaaag tccggccaca ttctatccat gcattaatgg
    19081 gggaaaacgg tgcaggaaaa tcgacattat taaaatgcct gtttggtatt tatcaaaaag
    19141 actccggcac cattttattc cagggtaaag agatcgattt ccattccgcc aaagaggcac
    19201 tggaaaatgg tatttcgatg gtacaccagg agttaaacct ggtattacaa cgttcggtga
    19261 tggataacat gtggctgggg cgatatccca ccaaaggcat gtttgtcgat caggacaaaa
    19321 tgtaccgcga aaccaaagcg atttttgatg aactggatat tgatatcgat ccgcgtgcgc
    19381 gcgtcggcac attatccgtt tcgcaaatgc agatgatcga aatcgccaaa gcgttttcct
    19441 ataacgcgaa aattgtgatt atggatgaac cgacttcttc gttaaccgaa aaagaggtca
    19501 atcatctgtt cactattatt cgtaaattaa aagagcgcgg ctgcggtatt gtttatatct
    19561 cgcataaaat ggaagaaatc ttccagttat gtgatgaagt taccgtattg cgcgacggtc
    19621 agtggatcgc caccgaaccg ctggcaggac tgacgatgga caagatcatc gccatgatgg
    19681 ttgggcgttc tcttaatcag cgttttcctg acaaagaaaa caagccgggc gaagtcatcc
    19741 tcgaggtgcg taacctaacg tcactgcgcc agccgtcgat tcgcgatgtc tcgtttgatc
    19801 tgcataaagg ggagatcctc ggtattgccg ggctggtggg ggcgaaacgt accgatattg
    19861 ttgagacgtt atttggtatt cgcgagaaat cggctggcac cattacgttg cacggcaaac
    19921 agatcaataa ccataatgcc aacgaagcca taaaccacgg atttgcactg gtaactgagg
    19981 agcgccgctc aacgggaatt tatgcctatc tggatattgg ttttaactcg ttaatttcca
    20041 atattcgcaa ctacaaaaat aaagttggtt tactggataa ctcgcggatg aaaagcgata
    20101 cccagtgggt gattgattcg atgcgggtaa aaacgccggg tcatcggacg caaattggtt
    20161 cgctctccgg tggtaatcag caaaaggtga ttattggtcg ctggctacta acgcaaccag
    20221 aaatattaat gctcgatgaa ccgacgcgcg gtattgatgt cggggcgaag tttgaaattt
    20281 atcagttaat tgccgaactg gcgaagaaag gcaaggggat tattattatc tcctctgaaa
    20341 tgcctgagtt gttagggata acagaccgta ttctggtcat gagcaatggt ctcgtttccg
    20401 gaattgtcga tacaaaaaca acaacgcaaa acgaaattct gcgtcttgcg tctttgcacc
    20461 tttaagatta ggggcttccc atgagtgcgt taaataagaa aagttttctt acttacctga
    20521 aagagggcgg tatttacgtc gttcttttag ttttgctggc gattattatt ttccaggacc
    20581 caacattttt aagtctgttg aacttaagta atattctcac ccagtcatcg gtgcgtatta
    20641 ttatcgcgct cggtgtggca gggttaattg tcacccaggg gaccgatctt tctgctggtc
    20701 gtcaggtagg gctggcggca gtggtggctg cgacattatt gcagtccatg gataacgcca
    20761 acaaagtgtt cccggaaatg gcgacgatgc cgattgcgct ggttattctg attgtctgtg
    20821 ccattggtgc ggtgatcggt ttgatcaacg gtctgattat cgcttatctc aacgtgacgc
    20881 cgttcattac cacgctcggc acgatgatca tcgtctatgg catcaactcg ctctattacg
    20941 actttgtcgg ggcgtcgcca atttctggtt ttgacagtgg cttctctacc tttgctcagg
    21001 gctttgtcgc gctggggagt ttccgtctct cttacatcac cttctacgcg ttgattgcgg
    21061 tggcgttcgt ctgggtgttg tggaacaaaa cccgcttcgg taagaacatt tttgccattg
    21121 gcggtaaccc ggaagcggca aaaatatctg gtgtcaacgt cggcctgaac ctgctgatga
    21181 tctacgcgtt gtctggcgtg ttctatgcct ttggcgggat gttagaagcc ggacgtatcg
    21241 gctctgccac caacaacctc ggctttatgt atgagctgga tgctatcgcg gcgtgcgtgg
    21301 taggcggtgt atcgttcagc ggcggtgtgg ggacggtgat tggcgtggtg accggggtaa
    21361 ttatttttac cgtcatcaac tatggcctga cgtatatcgg cgtaaaccca tactggcagt
    21421 acatcatcaa aggggcgatt attatcttcg ccgtagcgct ggattcactg aaatacgcgc
    21481 gtaagaagtg atgtgattgc ggatattgcc ggatgcgtct ctggttttac aacgttaccg
    21541 ggtgttcttt ctcgcctttc ttaaacttca cttccccgag ctcaatacaa cccaccgggc
    21601 agacgtgacc acaaagcaga caacccacac atttctcggt attacaatgc ggtgtgcggg
    21661 ttttctcgct ccattccatc gcctggtgac cgccgtcgta acaggaaata taacagcgtc
    21721 cacagccaac acatttgtca agattgatac ggggatagac aatataactg cggtctaaat
    21781 cttccgccgg gacaatattg ttattcgcca gacctaccat ctcctgcaac gaatcaaatc
    21841 cctgatcggc gagataatgc gataacccgc tcgccatatc ttctacatgc gatagccgta
    21901 ctgcataatg ccggtggtga cctgtaacgt tgctgcgccg agcaataaaa actccgccgc
    21961 atcttcctag gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg
    22021 ggtgcgcatt tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc
    22081 ggaaatactc gatttaccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt
    22141 ggtgatggat ttaacggtgt taattgccgc aatgccatct gcgccgccgc gcttcgccgc
    22201 cagcgccact tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt
    22261 ggaaccccgt ttcactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc
    22321 gctacccatc gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc
    22381 gccagctgct cccattgctg ctcattttcc cccatgatcg aagcgatcaa tactttgtcc
    22441 gggtaatctt ccttcagccg acgcagggcg gtcagattct cttccaacgg atgttcagca
    22501 atctgctcca tatttttgaa gccgataaaa ccggtatctt ctttcaccag atgatcaaaa
    22561 cgcggcgaga cttcgttggc gataaaaaag ccgatcgttt taaacaccac accgccccaa
    22621 cctgtgtcgt aggctttggc acacatctca tagcagttgc ctaccggcga agaagagagg
    22681 cagaacgggt tgggaaagtt cacgccgcaa aaagtaatcg aaagatcttt cgttaacatg
    22741 agcaagctcc ctctaaatag tgatgaatcg cctcggcggc ttctttcccg gttttcacgg
    22801 catagaccac ggttttgcca ccctcaacaa tatcgccagc agcaaagact tgcggatcgc
    22861 gggtctgata attttgtgtt ttgatggtgt tacgctgctg ctctaactcc gcaaaggcat
    22921 ccagtctggc atgctgaccg acggcgagaa taattttatc tgccgccatc gtcagttcgc
    22981 ctgataaccg tacatgctta aacgtgactt tattgccttc cacggctact ggcgtgaatc
    23041 catcgatgat cgaaacgccc agctcccggg cgctggcaaa ctctttttcg cttgccggaa
    23101 actcatctaa ctcttcacgt gctacgcaag ttaccgcctg acagccgaga actttcagcg
    23161 tgctggctac gtccatcgcg acatcaccgc cgccgataat taatgcgctt tgtggaatgc
    23221 tgatatcgcc ttgcgcctgt cgtacacgtt gcaggaagtc gacggcaatc tcaacgtcgc
    23281 tatgctcaaa cagcggtagc ccggaaccgc ttgataaccc cacggtgacc agtaccgcgc
    23341 ggttttccgc tttcagttgc tccagggtga gtgttttacc gatttcgtta ttgcacttaa
    23401 tggtcacgcc cattttttca atacgggcga tctctgcatc cagcactgat tgcggtaacc
    23461 ggaattgcgg aataccgtta cgcaaccagc caccggggtg cgcttctttc tcataaatcg
    23521 tgacgtcata accctggttt gtcagtgtca cactggcctg caatccggca ggacctgcgc
    23581 caataatcgc gactttgccg agcgttttag taccgggctg ataaatttcc atcccggttt
    23641 gttgttcaaa atcagtaaca aaacgctgta agcggccgat atcaatggga gcatcaacac
    23701 cggcacgggt acaaccgctt tggcataatt tctccgtcgg gcacactctg gcacaaacgg
    23761 caccgagggc attattttcg cgaattgtct cggcagcgcc tttaaaatta cgaaagtaga
    23821 ttgagcgaat aaatttcccc ggatcggtct gtgccgggca agcctgacta cagggagcgt
    23881 cgtgacataa taaacagcga gaggcttctt taatcgccag taacggcgta aaagccggag
    23941 tgagttcatc cagataattt tgttgcggca tgtgatcttc cttaatgcta tgagtaatta
    24001 tggcgaacgc taaataaata ttgcgtgctg atattaaggc tatgcgcgtg gggaaattta
    24061 agctgtgaaa ggattcacaa atagctgcgg ttcagaatgt tatatccgca gcttattgag
    24121 tgaagtaata attattctca ataattaaca gaaaagagaa aatattaacg atagaaaata
    24181 agataaacaa tcatgccgat agataaaatt ccgaagccaa tatatgccac catataagcg
    24241 aagaagagtc cgggattttt tgctcgccag acatacaccg gttccggtgc atttttaacc
    24301 cgtttatcaa tcgcgcctgc gcgccagcct ttccatgctt cgcggaaaca aaatatcggg
    24361 ataaggaaaa aaacggcaac gacaaaaaac tgctgtacat ccataactac tttccttgtt
    24421 tcttttgtaa ttcaagtaac tgttcgggtg taacggcggg atacccctgc gcatcttccg
    24481 gcacctggtg catagccgga atagggacca gcggccctaa aaaacgcggt tcacgtttaa
    24541 aaatataaag gtcagccagc gcaccgaaac gggcggcaaa ttcgcgaata cgtactgaca
    24601 gcatatcttt cggtgacggt acggcataac actgagcctg aatccccata tgcagcgcaa
    24661 taaataatgc tcgctcacag tggaaacgtt gggtgataat aatgaaatca ttagtatcga
    24721 aaactttgcg tgtacgcacg atggaatcca gcgtacgaaa gcctgcgtaa tcgagaacaa
    24781 tatctgatgg gtcgacacca gcagcgatta aatctttgcg catggtcatc ggctcgttat
    24841 aactttgcaa tgcgttatcg ccgctcagta atagataatt taccttaccg ctgttatagg
    24901 cattaatcgc tccttgaatg cggtagcgat aatactgatt aataacgccg gtacgataat
    24961 attttgctgt gccgagcacc acgccgacct ggcggtaggg gagatcctgc aattcgtcgt
    25021 agatataagg cgcggttttc cagctcatcc agcgatcgag gccgagcaca gtcaacagca
    25081 gcaagccgat caggactaac aggctgagga acacgcgctt taacatgcag gagactcgag
    25141 ctaaggaaaa tttccatcag gctactttac ccgctggttt tgcgcaagaa acggcaaact
    25201 gtttgccggg aatttcgaca ccaggagagg gctggcaagc cggacaaggc gttcacgccg
    25261 catccggcac caggcttaag cgagaagcac gcggtcgata ctgtgacagc cgagagcttt
    25321 cagcgttgcg gaggtggcat cccactgaat caacggcgca tcggcttttt ctgccagaac
    25381 cgcgcgctgg atatccgggt aatcataacc cttgagattc aacagaatta acgccccctg
    25441 caacggcggc agagtcgggt tgaatgcggc gttttcagcg tagctgccgc tgaaaatacg
    25501 accgtcttta cattccagcg cgacaccgct tggtgactta ctgtaaggca tgtgcgaacg
    25561 gtttgccgct gcaatcgccg cctgagaaag cgcatcacca gtcagcgcat agccgtgatc
    25621 ctgctcgtcc atcagcagcg ttttaatctc cagatctttc ggcccaaagg catccggcag
    25681 atagtcacgc agcgcgtgtg cctcgcggcc cggcaaatga atacgcagat ccagaccgct
    25741 gttcagttca ttcataaact gacggcagtg accacaaggc gtatagttaa cggtgatggc
    25801 tgcaagcgct ttttcaccac tcaaccaagc gtggctgatt gcgctttgtt cggcatgaac
    25861 ggtttgctgc attgttgcac caataaattc catattggca ccaaaatacc aggttccgct
    25921 cacaccgcgc gcaattgcgc caacattaaa attcgacaat ggcgtacgcg cacaggccgc
    25981 cgccagcgga agtagtgcga atgccagcgc gtcttcgtcc agtcccgttg cgctcttcag
    26041 cgatgagact tgctccccgg tcaacaaagc ggggaagtac ttgtctgcca gaataggttc
    26101 cagtgcagat tgcaagttat ccgcaagttg ggcaaaagcg gtttgaaaac gtggatgcat
    26161 ggcgttgcct cataatcatg taatgggata acagtttacg aacccgttat ggctttatat
    26221 gtgatctgaa tctcattaat tatgcaacac gtgaaactta catcaaaaat gcgcgacgta
    26281 tcgcaaattt tagcccatta ctgccagaat aatcgggaaa aggaacggtg cgatcagcga
    26341 agtaattatc ccgcataaca ccagcgctag cgaactaaat gcaccttcct gataatccag
    26401 ctcggcgcag cgcgccgtac cgagggcgtg cgaggcagtc cccattgcca gaccgcgcgc
    26461 agctttggta cgaatacgca tcgcattaag caatgtatgg ccaaataccg cgccgaggat
    26521 gccgacgaaa atcacgcaaa ctgcgctgat tgccggaata ccgccaatac tgccgccaac
    26581 cgccattgca ataggcgtgg tgactgattt cggcaggatt gacgcggcga tttccggtga
    26641 agcgcccatc aacaatgcca cggaagtccc cgtcaccatc gcaaccacgc tgccgataaa
    26701 acagatggtg atgatcgatt tccagcgcgc gcggatctgg tgtagctgct catataaagg
    26761 ataggccagc gcgacgaccg ccggttgcag cagatcgttt agcacttcgc tacctttaaa
    26821 gtaactgtcg taagagatgc cggtcagcat taaaaaagga atgatcacca ccattgctac
    26881 cagcaacggg ttaagcaggg gaaacttata ccgtgccgcc agtttgcggg cggcaaaaaa
    26941 gacaatcaac gtcagcggta atgaccacca gatattcgcc atcattcttc tgatcctttc
    27001 tgacctacga ctttacgttc accgtgtacc agttgtgaac tccagctcac caccagaaaa
    27061 accaccagcg tactgacagc acaggaaacc actaccgggc caaactgcgc gcggagcaaa
    27121 tcaaaatatt gcatgacgcc tacgccaatc ggcacaaaca atagcgccat atagcgaatc
    27181 agtacgtagc accccggatt gacccatttc gccggaagaa tttgcaaggc cagcaggaca
    27241 aacaggatct gcatcccgat gatgctgccc ggaatggtta ccggtagcag ggaagcaatg
    27301 aaaatgcctg catacaggca ggcataaatc aggatgaaag cgcgtaaata ttgccagata
    27361 atgttcagtg tcttactcat aatcaacacc ccagatgaga tggattcatc atacaattaa
    27421 ttcgtaaaac gtgctaccga tcacattaag aaatcagcgc ttattttttc gctacccggt
    27481 attacgcaat gagcagcgaa ctcaggcata catcaagcat agagagggat tgcagtttgc
    27541 agcaatccct ctttgcagac ctttaatccg ctgaccatag gcgctaacat agcgcctcat
    27601 ttttttgcgg gtgatgatat gcgtgtgtta ctggcaccga tggagggagt gcttgactct
    27661 ctggtgcgtg aattgctgac cgaagttaac gactacgatc tgtgcatcac cgagtttgtc
    27721 cgcgtggtgg atcaactgct gccggtaaaa gtctttcatc gcatttgccc tgagctacaa
    27781 aacgccagcc ggacaccatc tggtacgctg gtgcgcgtgc agttgttagg tcagttccca
    27841 caatggctgg cagagaacgc cgcccgtgcg gtggagttag gtttctgggg cgtggatctc
    27901 aattgcggct gcccgtcgaa aacggttaac ggtagcggcg gcggggcgac gttactcaaa
    27961 gatcctgaac tcatctacca gggggcaaaa gcgatgcgtg aagctgtacc ggcgcatttg
    28021 cctgtcagcg tgaaagtgcg tctgggctgg gacagcggtg agaagaaatt tgaaatcgcc
    28081 gatgcggttc aacaggctgg cgctacggag ctggtggtgc atgggcggac gaaagagcag
    28141 ggttaccgcg cggagcatat tgactggcag gcgattggcg agattcgcca gcggctgaat
    28201 attccggtga ttgccaacgg tgaaatctgg gactggcaga gcgcgcaaca atgcatggcg
    28261 atcagcggct gcgacgcagt gatgattggt cgcggggcgc tcaatattcc caacctgagc
    28321 cgggtggtaa aatataacga accgcgaatg ccgtggccgg aggtggttgc tttgctgcaa
    28381 aaatataccc gtctggaaaa gcagggcgat accgggttat atcacgttgc gcggattaaa
    28441 cagtggttga gttatttgcg taaagaatac gatgaagcaa cggaattatt tcagcatgtt
    28501 cgggtgttga ataactcccc tgatattgca agggctattc aggcaattga tatcgagaaa
    28561 ctctaaaaac catagtgatg tttttgacaa tgtgttagct ctttatcaat aggccgcacg
    28621 gttgtttggt ggcgtcaaaa tcgccgaggc gttccctgaa ggccggggca gcccacatgg
    28681 atgtgggctg agggcgcgtt ttacagggat gttacctcgc gcccgacccg gtagccgtaa
    28741 gggataagcc gagggcaccg cgcagcggcg attttgttcg ccagagcccg ggggggcagg
    28801 gggcggcggc gattggtcgc cccctgcgcg ctccttgcgc cagtgattat atgaagcaat
    28861 gcacatgaca ggagcgcaac aggatgagag actgaaatat tctcgttatt ttcggatctg
    28921 atgacattaa ttaattttaa aatatcatct taaatacgcc agtcaccacc agtagcccaa
    28981 tcaggaaaat aatcaataca gcccagagta taattttcat tggcgttcct ttatttgtga
    29041 gtgctgaata agtataggaa aaaacgaagt cctttgccgt tatccataaa cgattaagat
    29101 aaatcggaag atatcattta cttagtctat atttttctgg gtatgtcgtt ttattgctac
    29161 tgatgtattg ccacgcgaaa ctaaaaaata tatcgcaaca cacggtaatc tccgcgcacc
    29221 gtttcttacc tgattattcc cccatgaatc gtgattcctt ttatccagcc atcgcctgtt
    29281 ttccgctgtt actgatgctg gccgggtgtg cgcctatgca tgaaacccgc caggcgttaa
    29341 gccagcaaac gcccgctgca caagttgaca ccgcattacc cacggcgctg aaaaatggtt
    29401 ggccagacag ccaatggtgg ctggagtatc acgataatca gctcacttca ttaattaaca
    29461 atgcactaca gaacgcaccg gatatgcagg tggctgaaca gcgcattcag ctagctgaag
    29521 cccaggcgaa ggcagttgcc actcaggatg gtccgcagat cgacttttcg gcggatatgg
    29581 agcggcaaaa aatgtcggca gaagggctaa tggggccgtt tgctctgaac gatccggccg
    29641 caggtacgac cggtccgtgg tacaccaacg gaacttttgg cttaacggcg ggctggcatc
    29701 tcgatatctg gggaaagaat cgggcggagg ttactgcccg cctgggtacg gttaaagcac
    29761 gggcggcgga acgcgagcaa acccgccaat tgctggctgg cagcgtagcc cgcctgtact
    29821 gggagtggca aacccaggcg gcgttaaaca cggtcttgca gcaaatagaa aaagagcaga
    29881 acaccattat cgcgaccgat cgccagctat atcagaacgg gattacttct tcagttgacg
    29941 gtgtggaaac cgatattaat gccagcaaaa cccggcagca gctcaacgat gtcgcgggga
    30001 aaatgaaaat tattgaggca cggttaaatg cactcacaaa tcaccagact aagtcattga
    30061 agcttaaacc ggtcgcgttg ccgaaagtgg caagccagct tcctgatgaa ctggggtact
    30121 ccttactggc ccggcgggca gatttgcagg cggcgcactg gtacgttgag tcatcgttaa
    30181 gcactattga tgcggcaaaa gcggcatttt atcctgacat caacctgatg gccttcctgc
    30241 aacaggatgc gttgcactta agcgatctgt tccgtcattc cgcgcagcaa atgggcgtta
    30301 cggcaggcct gacgctacct attttcgata gtggtcgtct taacgccaat ctcgatatcg
    30361 caaaagccga aagcaacttg tctatcgcca gctacaacaa agcggtggtt gaagcagtga
    30421 atgacgtggc gcgggcagcc agtcaggttc agacactggc ggagaaaaac cagcatcagg
    30481 cgcaaattga gcgcgatgcc ttgcgtgtgg taggtcttgc gcaggcccgc tttaacgcgg
    30541 gcatcattgc tggttcccgc gtcagcgaag ccagaatccc ggcgctgcgt gagcgggcca
    30601 atggcctgtt attgcaaggg cagtggctgg atgcctccat tcgactcacc ggtgcgctgg
    30661 gcgggggtta caaacgctga tgatatgcgc cttctatact taacgtttat tcagcgttaa
    30721 gtggagaact cgatggcata ggtcgcgata attaccgctt ccgattcggg gatcggcaaa
    30781 gagtgcgcgt tattactggc gcagcagggg tttgatattg gcattacctg gcactcagat
    30841 gaagaagggg caaaagatac cgcgcgtgag gtggttaccc acggcgtacg tgcggagatc
    30901 gtgcaactgg atctcggcaa tctgccagaa ggtgcgcagg cgctggagac actcatccag
    30961 cgatttgggc gtattgatgt gctggtgaat aatgcgggtg caatgaccaa agcgccgttt
    31021 cttgatatgg cttttgatga gtggcgcaag atttttaccg ttgatgttga tggtgcattt
    31081 ttatgctcgc aaattgcggc tcgtcagatg gtgaaataag ggcagggcgg tcgcatcatc
    31141 aacattacgt cggtacatga acatacgccg ctgccggatg ccagcgccta cacagccgct
    31201 aaacatgcgc tcggcgggtt aaccaaaacg atggcgctgg agttggtcag gcataagatt
    31261 ttggtgaacg cagtcgcgcc tggggcgata gccacgccaa tgaacagcat ggatgatagc
    31321 gacgtgaagc ccgacgcgga gccttcgatt cctctacggc gttttggcgc aacgcatgag
    31381 attgccagcc tggtggtgtg gctttgttcg gagggcgcaa attacacgac agggcagtcg
    31441 ttgatagtgg atggcggctt tatgttggcg aatccgcagt tcaacccaga atagcgcagt
    31501 gaggtgcgtg gatatttcaa tgggttaggc cagataagac accagcgtcg catcaggcat
    31561 ggaacacccg gtgccggatg tggagtgaat gcttatctgt ggcaactgct ttacgcctga
    31621 ttatccggct ttttcttccc gcgccgtttc agccaccagc gcacgcccac caccagaacc
    31681 acgaccagaa tcaaccagac ccagtgcttc aaatgctggt cgagattgtg caaccacggc
    31741 gcaatcacct gaccaccagc gtaaccaata gtggtaaaaa tcaacgccca ggcaaatgcg
    31801 ccgagaatat tcagcggcag aaagattttc ggcggcagct ggctggcacc aatcagcgtc
    31861 gggccaatca cccgaaagcc atacataaag cgcgtaccaa tgacaaacag atacggatgg
    31921 cgttggataa gtttctgtgc ccgctcaatt ttatcctgat gtttcgagaa acggcgtaac
    31981 agcttgccgc caaaccgccg cccgcacaga tagagcacct ggtcgccaat catgccacca
    32041 agcgccacag aaagtaccac cagtgggaac tttaatagcc cctgatgtgc cgcaacgcct
    32101 cccagcaaag tcacggtttc accttccgcc aggctaccga taaccagcgc ggcataacca
    32161 tattgtgaga tgagtgtatt gagatccata ggccagtttg tctccctttg gttgcctgta
    32221 agcatacacc ctgtgaatca ttcacgctga aaaaggacat acatcagacg gtgcaaatta
    32281 catggatgaa caaaaaataa tcacaagctg gcttatactt gaactgtcag gttcatgaac
    32341 tcacaacaag ggggcggtat gagccatgtc tggggactgt tttcccatcc cgatcgtgaa
    32401 atgcaggtga ttaatcgcga aaacgaaacg atttctcacc attacaccca ccacgttttg
    32461 ctgatggcgg cgattccggt gatttgcgcc ttcattggca ctacacagat tggctggaat
    32521 tttggcgatg gcactatcct gaagttatcc tggtttaccg gactggcgct ggctgtcttg
    32581 ttttacggcg ttatgctggc tggggttgcg gtgatggggc gggtcatctg gtggatggcg
    32641 cgcaattacc cgcagcgtcc gtctcttgcg cactgcatgg tcttcgcggg ctacgttgca
    32701 actccgctgt ttttaagcgg tctggtggcg ctttatccac tggtctggtt gtgtgcgctg
    32761 gtcggcacgg tggcgctgtt ttacaccggc tacctgcttt accttggtat tccgagcttc
    32821 ctgaatatca acaaagagga aggccttagc ttctccagtt cgacgctggc gattggcgtg
    32881 ctggtactgg aagtgctgct caccctaacc gttatactat ggggctacgg ttatcgtttg
    32941 ttctagaaag ttctttgacg ttgcattgct ggcgtaaatg tcagcaatgc agcatttact
    33001 cacgattctc ctttgacgat cgcacttttt gctcgttatg atggcgtcgc tagcctcagt
    33061 aaatccttac tccggcggtg cgcaacccgt gcgcgtgaac cactatctga atgctcatca
    33121 tgccgaaatt tcgagtttct ttatttagct tggccctgat gctggctgtg ccttttgcac
    33181 cgcaggccgt tgcgaaaacg gcagccgcta ccaccgcttc acaaccggaa attgcctccg
    33241 gtagcgcgat gattgttgat ctgaatacca acaaagtgat ctattcgaac cacccggatc
    33301 tggtgcgtcc gattgcgtct atcagcaaat taatgaccgc gatggttgtg ctggatgcac
    33361 gactgccgct ggatgaaaaa ctaaaagtgg atatcagcca gacgccggag atgaaagggg
    33421 tctattcgcg cgtacgactg aatagcgaaa tcagccgtaa agatatgctg ttgctggcgc
    33481 tgatgtcttc agaaaaccgc gcggcggcaa gccttgcgca ccattatccc ggtggttaca
    33541 aagcctttat taaggcaatg aatgcgaaag cgaaatcgct cggaatgaac aacacgcgct
    33601 ttgttgaacc taccggattg tcggtgcata acgtttcaac tgcccgtgac ttaaccaaac
    33661 tgctcattgc cagcaaacaa tatccgttga tcgggcagtt aagtactacc cgggaagata
    33721 tggcaacctt ctctaacccg acgtatacgc tgccgttccg caatactaat catctggtgt
    33781 atcgcgataa ctggaatatt cagttaacca aaaccggctt taccaatgcg gcgggccatt
    33841 gtctggtgat gcgtacggtt atcaataata aaccggtggc gctggtagtg atggacgcgt
    33901 ttggcaaata tacccatttt gccgatgcca gccgcctgcg tacctggatt gaaaccggta
    33961 aagtgatgcc tgtgccggca gcagcgttga gctataaaaa acaaaaagcc gcccaaatgg
    34021 cggcggcggg gcagacggca cagaacgatt aagtgaattc ggatggcgat actctgccat
    34081 ccgtaatttt tactccactt cctgccagtt tttccgttta ctggttttac cgatccccgg
    34141 attcatgcta ttggtcggat cattctcgcg atagaacttc tgcaacgtct ccggtgcttt
    34201 atacaaatga ccgacgttat gctcggcagg gtactgcgcg ccgcgctgct gtagcagttc
    34261 cagcatctgt tcttttaacg catgcacatc cacgcctttt ttcactatgt aatcctgatg
    34321 gaagacatag cacataaaat ggccgtaata gagcttgtgc accagctggc tgtcgatctc
    34381 cggcggtaaa tgctcatacc actcggtgtc gttacgccgc agagcgatat ccagtgccag
    34441 aatgtcttcg acttcatcgg aatgcaccgc ctgataacga attgctgcgc ccgcagcggc
    34501 gaaacggtgt aaaaacgctt tgctaccttc ctccggcgta cagacaaaga aatcgccctc
    34561 ggcctgtttg aaataatcca ccagccacga tttggcttca ccgacgccat cgcctgccat
    34621 ttttaacagc agatgatgct cgtatttatc gcgccagttt ttcatgcgcg gcggtaaatg
    34681 gctggggaac aggtgaccga atttttgcat tgcacggtca gtaaaatgcg gacggaagaa
    34741 tttcactttc tccagcatcg catcggtgcg tcccttgaga ttaaagaaga acggcatctt
    34801 gtcggtgcct aacttatcaa tcatcaggaa ggtgtctttg ccgtattttt ccgcaatatc
    34861 gtagatatcc cggtgcatat attccccggc aaccggcaga ttttcgaagt tagccagaat
    34921 atgacggcgg atttcggtca gcacttccgg ctggttggtg ccgatataaa acacctgctg
    34981 atttttttcc gcttcgaagg tatcaagacg taccgcaaag acggccagct tcccggcgca
    35041 accagaagat tcaaataacc gatccggatc ggcgttataa cgtgcgggcg tgtcggcttc
    35101 aatatcacga acgcggtgga catagtcata atcgtgggcg tgacgaccat cgtgacgcac
    35161 atcgtcatct ttgatgcgat cgtcatccag cttgctaagg atctgctccg gcgtttcgcc
    35221 cagatcaatc cccagatggt tcaccagcgt cagtttgcca tcttcatcta tacgcgcgaa
    35281 taacgacatt tcggtatacg ccgggccacg ttgcaccagc gagccgccgg agttgttaca
    35341 aataccgccg atgaccgatg cgcctataca cgatgatcca atcaccgaat gcggttcgcg
    35401 tcccagcggt ttgagggctt tttccagcga atagagcgtg gtgcccggat aggccagcac
    35461 ctgttcgccc ttgccgagaa cgtgcagctt gtcgagacgc agggtgctga tgatcacgat
    35521 atcgcgatca tagtcgttac cgtttggcgt cgatccttcg gtcaggcctg tattggcagc
    35581 ctgcatcaga ataattttgt cggcggtgac gcaggctttc agcacccgcc acaattccag
    35641 tagtgagcca gggaaaacga cagccagcgc gtcgccctga ccagaacgga agcccttgcg
    35701 atagcgggcc gtttttgcgg gatcggtgag caggtgtgaa tgaccgacca gacgggcaag
    35761 ttcattcaaa aaggctttat tatcagttgt tgtcatggaa gacattttcc actccttgtg
    35821 gtggcgaaaa aatatcacaa actagaatag cgccttgtat gacaattcag cgagtgaata
    35881 ttttgaaaat tcagacaata aactgagcat tctttttctc tctccatcat gcttatggca
    35941 aactacggtt tttgcaccat tttcgctgcc acgtcgggca acaaaggaag aaaaatccat
    36001 atgaaatggc tatgttcagt aggaatcgcg gtgagtctgg tcctgcagcc agcactggcg
    36061 gatgatttat tcggtaacca tccattaacg cctgaagcgc gggatgcgtt cgtcaccgat
    36121 ctgctcaaga aaatgacggt tgatgagaaa attggtcagc tgcgcttaat cagcgtaggc
    36181 ccggataatc cgaaagaggc gatccgcgag atgatcaaag acggtcaggt tggggcgatt
    36241 ttcaacaccg taacccgtca ggatatccgc gccatgcagg atcaggtgat ggaattaagc
    36301 cgcctgaaaa ttcctctttt ctttgcttac gacgtgctgc acggtcagcg cacggtgttc
    36361 ccgattagcc tcggtctggc ctcgtctttt aacctcgatg cggtgaaaac ggtcgggcgt
    36421 gtctctgctt atgaagcggc agatgatggc ctgaatatga cctgggcacc gatggtcgat
    36481 gtctcgcgcg atccgcgctg gggacgtgct tccgaaggtt ttggcgaaga tacgtatctc
    36541 acctcaataa tgggtaaaac catggtggaa gcgatgcagg gtaaaagccc ggcagatcgt
    36601 tactcggtga tgaccagcgt caaacacttt gccgcatacg gcgcggtaga aggcggtaaa
    36661 gagtacaaca ccgtcgatat gagtccgcag cgcctgttta atgattatat gccgccgtac
    36721 aaagcggggc tggacgcagg cagcggcgcg gtgatggtgg cgctgaactc gctgaacggc
    36781 acgccagcca cctccgattc ctggctgctg aaagatgttc tgcgcgacca gtggggcttt
    36841 aaaggcatca ccgtttccga tcacggtgca atcaaagagc tgattaaaca tggcacggcg
    36901 gcagacccgg aagatgcggt gcgcgtggcg ctgaaatccg gaatcaacat gagtatgagc
    36961 gacgagtact actcgaagta tctgcctggg ttgattaaat ccggcaaagt gaccatggca
    37021 gagctggacg atgctgcccg ccatgtactg aacgttaaat atgatatggg gttgtttaac
    37081 gacccataca gccatttggg gccgaaagag tctgacccgg tggataccaa tgccgaaagc
    37141 cgcctgcacc gtaaagaagc gcgtgaagtg gcgcgcgaaa gcctggtgtt gctgaaaaac
    37201 cgtctcgaaa cgttaccgct gaaaaaatcg gccaccattg cggtggttgg gccactggcg
    37261 gacagtaaac gtgacgtgat gggcagctgg tccgcagccg gtgttgccga tcaatccgtg
    37321 accgtactga ccgggattaa aaattccgtc ggtgaaaacg gtaaagtgct gtatgccaaa
    37381 ggggcgaacg ttaccagtga caaaggcatt atcgatttcc tgaatcagta tgaagaagcg
    37441 gtcaaagtcg atccgcgttc gccgcaagag atgattgatg aagcggtgca gacggcgaaa
    37501 caatctgatg tggtggtggc tgtagtcggt gaagcacagg ggatggcgca cgaagcctcc
    37561 agccggaccg atatcactat tccgcaaggc caacgtgact tgattgcggc gctgaaagcc
    37621 accggtaaac cgctggtgct ggtgctgatg aacgggcgtc cgctggcgct ggtgaaagaa
    37681 gatcagcagg ctgatgcgat tctggaaacc tggtttgcgg ggactgaagg cggtaatgca
    37741 attgccgatg tattgtttgg cgattacaac ccgtccggca agctgccaat ttccttcccg
    37801 cgttctgtcg ggcagatccc ggtgtactac agccatctga acaccggtcg tccgtataat
    37861 gccgacaagc cgaacaaata cacttcgcgt tattttgatg aagctaacgg agcgctttat
    37921 ccgttcggct atggtctgag ctataccact ttcaccgtct ctgatgtgaa actttctgcg
    37981 ccgaccatga agcgtgacgg caaagtgacg gccagcgtgc aggtgacgaa caccggtaag
    38041 cgcgaggggg ccacggtagt gcagatgtac ttgcaggatg tgacggcttc catgagtcgc
    38101 cctgtgaaac agctgaaagg ctttgagaaa atcaccctga aaccgggcga aactcagact
    38161 gtcagcttcc cgatcgatat tgaggcgctg aagttctgga atcaacagat gaaatatgac
    38221 gccgagcctg gcaagttcaa tgtatttatc ggcactgatt ccgcacgcgt taagaaaggc
    38281 gagtttgagt tgctgtaatg ctttagtaag ggcgcgacgt ttgcgccctt tgcctgatgt
    38341 tcatatctta acttgctgaa ttttcgccct ttgtaggccg gataaggcgc tcgcgccgca
    38401 tccggcagga aacgccggac cgaatatgcc tcttaagtat ttatctgatt ctgcaactga
    38461 atccttccgc tcaggctaac tctgtcatta tcaactatgc ttttctctta attcgctgaa
    38521 aacaacagta aaagaatgag gaaagcatca tgccactctt aaagctctgg gcaggttcac
    38581 tggttatgtt ggcagccgtg agcctgccgc tacaggcggc ttcccccgtt aaagtcggtt
    38641 caaaaatcga taccgaaggc gcgctgctcg gcaacatcat cttgcaggta ctcgaaagcc
    38701 acggcgtacc gacggtgaat aaagtgcaac tcggaacgac tcctgtgggg cgcggggcga
    38761 ttacttccgg tgaactggat atctatccgg aatataccgg caatggcgct ttcttcttta
    38821 aagatgaaaa cgatgcagcg tggaaaaatg ccgggcaggg ctacgagaaa gtcaaaaagc
    38881 tcgatgcgga gcacaacaag ttaatctggc tgacgcccgc gcctgcaaat aacacctgga
    38941 ctatcgccgt gcgtcaggat gtggcagaga aaaacaaact cacttcgctt gccgacctga
    39001 gtcgttatct gcaagagggc ggcaccttca aactggcagc ctcggcagag tttatcgaac
    39061 gcgccgatgc gttacccacg tttgaaaaag cctatggctt taagctcggt caggatcagt
    39121 tgctgtcact ggctggtggc gacacggcgg tgacgattaa agccgctgcc cagcaaacct
    39181 ctggcgttaa tgccgcaatg gcttacggca ctgacggtcc ggtcgcggcg ctggggctgc
    39241 aaaccttaag cgatccgcaa ggcgtgcaac ctatctacgc gcctgcacca gtggtgcgtg
    39301 aatcggtgtt gaaggagtat ccgcaaatgg cacagtggct acagccagtc ttcgccagcc
    39361 tcgatgcaaa aacattgcag caactgaatg ccagcattgc agtggaagga ctggatgcca
    39421 aaaaagtggc tgccgactac ctgaaacaaa aagggtgggc gaagtaattt cccgtgactt
    39481 atctacgtat taatcctgtt ctggcgctgc tgttgttgct gacggcaatc gcagcggcgc
    39541 tgccgtttat cagttacgcg cctaatcgtt tagtctcggg tgaagggctt catctctggc
    39601 aactgtggcc gcaaacgatc tggatgctgg tgggcgttgg ttgcgcctgg ctgacagcct
    39661 gttttgtccc cgctaaaaaa ggcagcattt ttgcactcat tctggcgcaa ttcgtcttcg
    39721 tattgttggt gtggggagct ggaaaggcgg cgacccaact ggcgcaaaat ggcagtgcgc
    39781 tggcgcgtac cagcctcggc agaggtttct ggctggctgc ggcgctggca ttgctggcct
    39841 gtagcgatgc catccgccga atctccacgc atccgctgtg gcgctggttg ttgcatatgc
    39901 agattgccat tattccgctg tggttgctgt actccggcac gcttaacgat ctctcactaa
    39961 tgaaagaata cgccaaccgt caggatgtgt ttgacgacgc gctggcacaa catctgacgt
    40021 tgctgtttgg tgcggtgctg cctgcgttag tgattggtgt gccgttgggc atctggtgct
    40081 acttttccac tgcgcggcag ggggcaattt tttctctgct caatgtcatt cagaccgtgc
    40141 cttcggtggc gctctttggc ctgttgattg cgccgcttgc cgcgctggtt acggcctttc
    40201 cgtggctggg gaagctcggc atagcaggaa ccggaatgac acccgcactg attgcgctgg
    40261 tgctctatgc cttgctgccg ctggtgcgag gcgtggtagt cggtttgaac cagatcccgc
    40321 gcgatgtgct ggagagcgcc agagcgatgg ggatgagcgg ggcgcagcgt ttcctgcatg
    40381 ttcagttacc gctggcgtta ctggtatttc tgcgcagcct gcgggtggtg atggtgcaaa
    40441 ctgtaggtat ggcggtgatt gcggcgttaa tcggcgcagg cggttttggt gcgctggttt
    40501 tccaggggct gctaagcagc gccattgatt tagtgttgct gggggtgatc ccggtaattg
    40561 ttctggcggt gcttaccgac gcgctgttcg atttgcttat cgcactgctg aaggtgaaac
    40621 gtaatgattg aatttagcca tgtcagcaaa ctgttcggcg cacaaaaagc cgttaacgat
    40681 ctcaatctca attttcagga agggagtttt tcggtgctga ttggcacatc tggctccggc
    40741 aaatccacca ccctgaaaat gattaaccgc ctggtggagc atgacagcgg agtgatccgc
    40801 tttgccggag aagaaattcg ctcgctgcca gtactggagt tgcgccgccg gatgggctat
    40861 gccattcaat ctattggcct gttcccccac tggagcgtgg cgcaaaacat cgctaccgtg
    40921 ccgcaattac aaaaatggtc gcgggcgcgg attgacgatc gtatcgacga attaatggcg
    40981 ctactggggc tggagtcaaa tttgcgtgag cgttatccgc atcagctttc cggtggtcag
    41041 cagcaacgtg tgggagtggc gcgtgcactg gctgccgatc cgcaagtctt actgatggat
    41101 gaaccttttg gcgcactgga cccggtaacg cgcggcgcgt tgcaacaaga gatgacgcgc
    41161 attcaccgtt tgctggggcg cactattgtg ctggttactc atgatattga tgaggcgcta
    41221 cggctggcag aacatctggt attgatggat cacggtgaag tggtgcagca ggggaatccg
    41281 ctgacgatgc tgactcgtcc ggcgaatgat tttgtccgcc agttttttgg acgtagtgaa
    41341 ctgggggtgc acctgctttc gttacgtagc gtggcggatt acgtgcgtcg cgaagaacgg
    41401 gcagaaggtg aggcactggc agaagagatg acgctacgcg atgcgctctc cctgtttgtc
    41461 gcgcggggat gcgaggtgct gccggtggtg aacacgcagg gccagccttg cggcacgctg
    41521 cattttcagg atctgctggt ggaggcgtaa gcgtatgaag atgttgcgcg atccgctgtt
    41581 ctggctcatt gctctttttg tggcgctgat tttctggctg ccttacagcc agccgctgtt
    41641 tgctgccttg ttcccacaac tgccacgacc cgtttatcag caagaaagtt ttgcagctct
    41701 ggcactggct catttctggc tggtgggaat ttcgagtttg tttgcggtga tcattggcac
    41761 aggtgtcgga attgctgtta ctcgcccgtg gggcgcggaa tttcgcccac tggtggaaac
    41821 tattgccgcc gttggacaga cttttccgcc tgtcgcagtg ctggcgattg ccgttccggt
    41881 gatcggcttt ggtctgcaac cagcgattat cgccttgatc ctttacggtg tgctgcccat
    41941 cctgcaggtg acacttgccg ggctgggagc gattgatccc agcgtgacag aagttgcgaa
    42001 aggtatggga atgagtcgag gtcagcgact gcgtaaggtc gagctaccgc tggcggctcc
    42061 ggtgattctg gcgggcgtgc gaacttcggt gattatcaac attggtacgg cgacgatcgc
    42121 ctcaacggta ggggccagta cgctgggtac gcccatcatc atcgggctta gcggatttaa
    42181 taccgcgtat gtgatccagg gggcgttact ggtggcactg gcggcgatca tcgcagaccg
    42241 cctgtttgaa aggctggtgc aggcgcttag ccagcacgca aaataaaggt ataacctgcg
    42301 agcatgacgc caccaattcc gcctaacgcc ataaacagga acagggcgat gaccccaatt
    42361 ttagctatgc gcataatgca ctccttatgt taacgaaagg attgtacagt aaagcgcatt
    42421 tgttaacgaa tcattaaata ccgagtggga aaatatcatg gccttgttct tgccaactgg
    42481 tgagttgctg ctgttgggcg gaggttcgat tttcaccgca ccacaccagc aatgtacggc
    42541 cttcgaatag ttcagggcgt agttgattga gcgagtgggc gagcgaggac atcaatgcgc
    42601 catccttgtt gactggcaat ccagccctcc agccacagac gggtggtatc ctgaatattc
    42661 cagccaacca ccagcgcatc tttaccctgt tttttacgtg ccgaagccag acaaatggcg
    42721 atgtagttga tcagtacgcc gtcgaggatc gccagcagcg cctggagagt cggttgttgg
    42781 cactgaagcc gtcggcgcag aggaataaac agatgtgtgg tgagtgtctg ggcggggtaa
    42841 tcctgaccgc gctctttgat ccacgttcgc aggctatgta gattgc
//
LOCUS       DAOFMX010000029        42871 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.029, whole genome shotgun sequence.
ACCESSION   DAOFMX010000029 DAOFMX010000000
VERSION     DAOFMX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.029"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="QBA20_001758"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="QBA20_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDQ6292326.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="QBA20_001759"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="QBA20_001759"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDQ6292327.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="QBA20_001760"
     CDS             complement(3736..4092)
                     /locus_tag="QBA20_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDQ6292328.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="QBA20_001761"
     CDS             complement(4096..4512)
                     /locus_tag="QBA20_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDQ6292329.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="QBA20_001762"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="QBA20_001762"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDQ6292330.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="QBA20_001763"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="QBA20_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="QBA20_001764"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="QBA20_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDQ6292331.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="QBA20_001765"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="QBA20_001765"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDQ6292332.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="QBA20_001766"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="QBA20_001766"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDQ6292333.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="QBA20_001767"
     CDS             10521..11504
                     /locus_tag="QBA20_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDQ6292334.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="QBA20_001768"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="QBA20_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDQ6292335.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="QBA20_001769"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="QBA20_001769"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDQ6292336.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="QBA20_001770"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="QBA20_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="QBA20_001771"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="QBA20_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDQ6292337.1"
                     /translation="MGKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="QBA20_001772"
     CDS             complement(15306..15515)
                     /locus_tag="QBA20_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDQ6292338.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="QBA20_001773"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="QBA20_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDQ6292339.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSIAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="QBA20_001774"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="QBA20_001774"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDQ6292340.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="QBA20_001775"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="QBA20_001775"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDQ6292341.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="QBA20_001776"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="QBA20_001776"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDQ6292342.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="QBA20_001777"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="QBA20_001777"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDQ6292343.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="QBA20_001778"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="QBA20_001778"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDQ6292344.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="QBA20_001779"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="QBA20_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="QBA20_001780"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="QBA20_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDQ6292345.1"
                     /translation="MKMNKSLIALCLSAELLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSRTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="QBA20_001781"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="QBA20_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDQ6292346.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="QBA20_001782"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="QBA20_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDQ6292347.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSSGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="QBA20_001783"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="QBA20_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDQ6292348.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="QBA20_001784"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="QBA20_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDQ6292349.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="QBA20_001785"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="QBA20_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDQ6292350.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32407..33882
                     /gene="xylE"
                     /locus_tag="QBA20_001786"
     CDS             32407..33882
                     /gene="xylE"
                     /locus_tag="QBA20_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HDQ6292351.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33926..34336)
                     /gene="psiE"
                     /locus_tag="QBA20_001787"
     CDS             complement(33926..34336)
                     /gene="psiE"
                     /locus_tag="QBA20_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDQ6292352.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34462..34605)
                     /locus_tag="QBA20_001788"
     CDS             complement(34462..34605)
                     /locus_tag="QBA20_001788"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292353.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34606..34884
                     /locus_tag="QBA20_001789"
     CDS             34606..34884
                     /locus_tag="QBA20_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292354.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34931..37027)
                     /locus_tag="QBA20_001790"
     CDS             complement(34931..37027)
                     /locus_tag="QBA20_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDQ6292355.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37027..37764)
                     /locus_tag="QBA20_001791"
     CDS             complement(37027..37764)
                     /locus_tag="QBA20_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDQ6292356.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37761..38399)
                     /locus_tag="QBA20_001792"
     CDS             complement(37761..38399)
                     /locus_tag="QBA20_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDQ6292357.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38513..38755)
                     /locus_tag="QBA20_001793"
     CDS             complement(38513..38755)
                     /locus_tag="QBA20_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292358.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="QBA20_001794"
     CDS             complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="QBA20_001794"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDQ6292359.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKIFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41391..42740
                     /gene="lysC"
                     /locus_tag="QBA20_001795"
     CDS             41391..42740
                     /gene="lysC"
                     /locus_tag="QBA20_001795"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDQ6292360.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10673 a        11026 c        10523 g        10649 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcaaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc cattggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa taatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gacaaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat gggaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgctatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaacagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 taataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagtggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgct
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg ggcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttctaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taactctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ctggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg
    32161 ttaatattcc catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa
    32221 ttctctttcg tgctctgagt cacggcaata gtattgtttt catcaatttt tgataattat
    32281 cacaattaag atcacagaaa agacattacg taaacgcatt gtaaaaaatg ataattgcct
    32341 taactgcctg acaattccaa catcaatgca ctgataaaag atcagaatgg tctaaggcag
    32401 gtctgaatga atacccagta taattccagt tatatatttt cgattacctt agtcgctaca
    32461 ttaggtggtt tattatttgg ctacgacacc gccgttattt ccggtactgt tgagtcactc
    32521 aataccgtct ttgttgctcc acaaaactta agtgaatccg ctgccaactc cctgttagga
    32581 ttttgcgtag ccagcgctct gattggttgc atcatcggcg gtgccctcgg tggttattgc
    32641 agtaaccgct tcggccgtcg tgattcactt aagatttctg ctgtcctgtt ttttatttct
    32701 ggtgtaggtt ctgcctggcc agaacttggt tttacctcta taaacccgga caacacagta
    32761 cctatttatc tggcaggtta tgtcccggaa tttgttattt atcgtattat tggcggtatt
    32821 ggcgttggtt tagcctcaat gctttcacca atgtatattg cggaactggc tccagctcat
    32881 attcgcggaa aactggtttc atttaaccag tttgcgatta ttttcgggca acttttagtt
    32941 tactgcgtaa actattttat tgcccgttcc ggtgatgcca gctggctgaa tactgacggc
    33001 aggcgttata tgtttgcctc ggaatgtatc cctgcactgc tgttcttaat gctgctgtat
    33061 accgtgccag aaagtcctcg ctggctgatg tcacgcggca agcaagaaca gacggaaagt
    33121 atcctgcgca aaattatggg caacacgctt gcaactcagg cagtacagga aattaaacac
    33181 tccctggatc atggccgcaa aaccggtggt cgtctgctga tgtttggcgt gggcgtgatt
    33241 gtaatcggcg ttatgctctc catcttccag caatttgtcg gcatcaatgt ggtgctgtac
    33301 tacgcgccgg aagtgttcaa aacgctgggg gccagcacgg atatcgcgct gttgcagacc
    33361 attattgtcg gagttatcaa cctcaccttt accgtactgg caattatgac tgtggataaa
    33421 tttggtcgta agccactgca aattatcggc gcactcggaa tggcaatcgg tatgtttagc
    33481 ctcggtaccg cgttttacac tcaggcatcg ggtattgtgg cgctactgtc gatgctgttc
    33541 tatgttgccg cctttgccat gtcctggggt ccggtatgct gggtactgct gtcggaaatc
    33601 ttcccgaatg ctattcgtgg taaagcgctg gcaatcgcgg tggcggccca gtggctggcg
    33661 aactacttcg tctcctggac cttcccgatg atggacaaaa actcctggct ggtggcccat
    33721 ttccacaacg gtttctccta ctggatttac ggttgtatgg gcgttctggc agcactgttt
    33781 atgtggaaat ttgtcccgga aaccaaaggt aaaacccttg aggagctgga agcgctctgg
    33841 gaaccggaaa cgaagaaaac acaacaaact gctacgctgt aatcttcctg tccagcacgc
    33901 cgcgccattt cggcgtgctg tctttttact cccgcttcag ccgtttcgaa ttacacagcc
    33961 acagggtgat caccagtaac aggatcgcag ccgagtagat caacacatcc agtggcgatt
    34021 tatgatcgac gatgatcaag cgcacaatgg cggtgatccc aatatagaca aagtaacgta
    34081 gcgggaagtg aaaaccggac tgaaagtact tcacaatcag cgcgataaat tcgaaataga
    34141 gaaagtaaac caccagtcct tctaccagct catatttgct ggtttgttct ggcgcgaaca
    34201 gcacatcagc cagatgcacc gtttctttgc cgaggaagac aaccaaaatc aggccgaggc
    34261 acagcaggcc aagattgagt acggtctgca aaatggtgga gataaactcc acgcgcggac
    34321 gagagagtga cgtcatagca gtacctcctt cacagtggcg agcttcctgt ataacgcaaa
    34381 aatgtgacgg agatctatat tttttgtgta tgttttgttc aacctggatt tggagcgcgc
    34441 ctccaccaaa gccggatata cctacccgta ggccggataa gccgcgcctg gcggcgcatc
    34501 cggcaagaca aaccgcacca aagccggtaa ttcaaaaatc acccattcag gtgattccca
    34561 gccaccagca atgcgcgaaa aatcgacaat gttcctctgg aacaaatgaa acgtaacctg
    34621 ataaaggtgg taaaaatgaa gccttatttt gctgctttga tgttatcagt ctctgttctt
    34681 cctgcttatg ctggtccgct cggtactgct gataaagccg acctaccgca aagcaatgtc
    34741 tccagcccaa tgatggccca gtcgctcagg caaccagacc tgcaaccgat atcgacggac
    34801 aggaagacag aatgttttcg cctttacaca ccagatcgta aaccgggggt gaactgcgtt
    34861 cctgatggct caacaggtca ttaagaatga gggtgccagt gagtgcgaaa actggcacct
    34921 ttcccatgac ttagcggaaa tttacgctac ggtcgctggt catgtcatac aactggaact
    34981 tacgcccaag ttgctgacca ccgtcacgcg tcagcggcgt ccagccaatc gccgcacggc
    35041 tgcgtgtcgg gccagacgag aagagatcca acggtaccga gacatacacg cctttggtga
    35101 agtccccttc gccgtactcc tctttcgaaa cgttggtgat cgtggcatag ccacccacca
    35161 ccacgccgct gtcaaagcgt ttagcgatct ccagcgtgcc gcctttatcc cctgccagat
    35221 actgcccgac gctggctttc accaacacgt cctgggcgaa agatggcgtc cagtaggcgg
    35281 tcagatgtcc ggttttcacg ctgtagtcgg tgaatttcat catatctttt gcactacgcc
    35341 agtcgcgctg tttaacgtag ttggcatcca gaccaaacgc ccagttactg tccagcggac
    35401 gatacaacac ttcggccccc gcaccgccaa acatggtttc gagataacct ccatagacct
    35461 gaccgtagaa gccgttgccc agatgctgga agtagttggc ttgcaggtta ttcacataga
    35521 catcgttctg cacatactcg cgcacatggg tacgaacgcg cggtaagtgc gagtcctgcg
    35581 gtggattggt gtagttaaac ttgtcgtagt tgttggcaag atttgcaaac aggctgccgg
    35641 tggtcagtag atggtccgtc agccacaaat ctgccgttcc catcacgccc agctgataca
    35701 tgtaaaagtt ttccgggcca ccgaccgact ggttcagcac cggatcgata tggaaatcaa
    35761 agcgtgattt gtcgatatac cagccctgct cggtggactt cggaaccact ggctcgacgc
    35821 gtttgtgcgc cagcgtcgtt tcgtggccca acggctctcc ggcgagatga tttttcaggc
    35881 tggcgacatc ggtttccgtc gtcacttgcg gcatgttaag gcgattttcc gtaatgcgga
    35941 tcgttttgat cccatccggc agatcgttca tcacgatccg attagcacgg atgatccctt
    36001 cgcgcgaatc acgatatttc acctgctcgc cggtaacata cagcgtatcg cctttcgcct
    36061 ggatctgcgg atcggcaagt ccggcgttgt atttcagcaa cgttaactga ttcgccacca
    36121 ccgaatgctg caaaatggca tcctgcggct gcggttgata ttgcgggcgg gcgttatcga
    36181 tgtaggacgg gcgcagatcg ttaaagttgg tgcgcagcgt gacgccaaac ataaaggtgt
    36241 tgccacgttc atagctaagg ttaacgtcgg cccaatcggt aacgcgataa atcgccccga
    36301 cgttaaactt acttttttgc tccagcttcc cggcaaaatc ctgctgataa ttattacctt
    36361 catactccag tttcagacgc agcggttgcc agggcgtctg gtattccacg ccgccaaaca
    36421 gtgaggcagg accgtggaac atctggctac cgtcgataga tcccgcctgt ttgtagctgt
    36481 tatcgcgata gcaatattta tcactggctg aacagagcgg attttttaca ttaccgctgg
    36541 tgcccagata tccccagccc aggccgagcg taaaatcgaa cggcccccag gctttgctgg
    36601 caacaagata ttccgcatca aacagccccg taccgccgat atcccgcgca ccaaccgcca
    36661 cttgcggcag ccagtaactc tcttcccaca aacgcagttt gagatcgaag gctttatctt
    36721 tatacgtttg atcgccagag aacgcttcga cgctgctgta ctgccgggtg cgcacgtcgg
    36781 tgtagcgcag cgttgtttcc agccacggga agagttgcac tgaagctgag taataacggt
    36841 actgatcgtt atcgcgatag ttcagactca actccccttc ccgtgccata cgcgcggtgg
    36901 gagtttgtaa taatcctacg ccaccgaaat ccgactgcga cggaccaatg ggcgcaggat
    36961 atgtttcgcc gtagcaagct gtgctaatgc ccagcgccag taagctaagc agatgtcttt
    37021 ttttcattat tggggtatcc gctgcgtcag agtctgaaga atgtcggcgt taagggcatc
    37081 aggcgtctca ctccagacgg aatctgcgag gccaacataa ataatgctgc cgggcatcgg
    37141 ctctacgtga cgcttgttcc agtaagccac cggcgctttt tgcgtgcgtc cgtccgggta
    37201 aacgacccac gcgtagctgc gatccgcacc gctgagcagg ctctggtcag agagatagct
    37261 cgccacgtcg cgacctggag tgaatggctg attgccagga tggctgataa gcccgaacaa
    37321 cgtgaccgtg gacggcggtg gtccgaccca cagcgtgtag ttgccctgca acggcgggtt
    37381 accgcgttcg gcaacgcgga cgatatcggg atcaagattg attttttgcc tgcctgtcac
    37441 tttcaatgcc tggatttgct ggcgtaaggc gttaatggcg gcggcatcgt cggtgctgga
    37501 atctgcgccc tgttctgcca gccgtgtcag caacgcctgc tgctgacgca atgctgccgc
    37561 cgttgccagc tcttcgctaa tcaccgcacc aggccaccag ctgtttgcca gtcgaggttg
    37621 ccctaccaga tcgattaaat gctctgcgcc cgttagcgtt ttggcctcac tgctgccatt
    37681 gctgaacacc ttgacggttc ctgccgcgaa gaccgatgac gctcccacac tcaaaagcaa
    37741 cgcgacaata gtttgtttaa tcattgtttt gccgccttga tgagagtggt tttcaccggg
    37801 aaataatccg cgccgagata ctgttccgac tggcgaattt gcccttcgct gtcgatccag
    37861 tagcggttat gccagcgcgc ctggtcggtg gagacttctt cgtccagcac gcgaaccagg
    37921 gtttcatcgc tgccgacctt gacggtatcg gtgccatccc atttgaagac tgagcgtgcg
    37981 gtggcgtagc gtacctgctg gtactcggtc cagcccatcg tgcgcgtcca tgttgcacca
    38041 tcaacaattt gtgcgggttt aatcagcggg tcggcggcaa ggttattcac ttcaatcagg
    38101 ttgtcgccgc caagcaaagt cttcaccaga cgaccatgtt gtgtgacaag agtggcttga
    38161 tcctgagtga cccatttttg ttgtccgtct tcagcgaagg ccagcaccac aaacaattgc
    38221 ggcccgccat taagctgcat gtactggctg gcgtagggca tattttgaat atcatcgtcc
    38281 gtcagctgta cgcctggcgt gccgaacaga ctgtcccaca gtgaattgcc cagctcttta
    38341 gtcgtggctg aacaggcctg taatagcagg cagataagaa tgagtgcagg tcgcttcacg
    38401 actcttctcc agaggggcag aataaccaca ccggagtgtg gttatggtaa atcccttcat
    38461 acttcaagcc acagatgcaa tgactcaaat tattttgggg atacatactt tattactggg
    38521 tactggtggt cgtggttgtg atggtcccgg tattagaacc atcaccgcca ccggttgccg
    38581 cgagcgcgac acccaccgcc gagcttacgg tgctgacgct ggtcgcggag gaactccccg
    38641 ccgaaaccga cgttgctgcc gactctgccg cttcgcccac ctgtacaggt gcagcccacg
    38701 cagaagtcgc cgcaagcgca gatatggcaa aaatgccata cagaactttt ttcataacaa
    38761 tttcccttca ttgaatgaat ggagatttac ccaaaaacat ttcgggcggg atcgagtata
    38821 cacaactaaa gcatgcgaat tacggcgcgg ggaattagcg atggagtttt aggataattg
    38881 gaatgaatat caaatataag tttcaactta tatttaaata acaataaaaa tcataagctt
    38941 aaaataaaaa caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc
    39001 tattttagga ttaacctcat ggtatgattt ccgttaaatc acagacaagg ggatttatct
    39061 gataaaacaa tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact
    39121 atgcattaat gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta
    39181 tccggcctac atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg
    39241 gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc
    39301 tggcagaata cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa
    39361 gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc
    39421 caggctgaac ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa
    39481 cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc
    39541 ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt
    39601 agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa
    39661 atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt
    39721 agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg
    39781 gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg
    39841 catatactgg tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca
    39901 gatgccaatc agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg
    39961 cttgtccatt gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag
    40021 aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa
    40081 ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag
    40141 cgccgcaaaa tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg
    40201 cgcgctatgg gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa
    40261 cagcgtggtt tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt
    40321 agaaacaaag tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta
    40381 cgggccgagg tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt
    40441 ataacccttc cactcaccgg agataatcgc ttccgagaag attttcatct tctccagcac
    40501 cgcgttgact tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt
    40561 acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa
    40621 catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag
    40681 cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc
    40741 ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt
    40801 catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt
    40861 tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag
    40921 tcacagaaaa atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc
    40981 gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg
    41041 accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag
    41101 cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata
    41161 acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta
    41221 caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg
    41281 gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg
    41341 ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa
    41401 ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg
    41461 ctgatattgt gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta
    41521 tcactaatct gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac
    41581 tcgacgctat tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta
    41641 tccgtgaaga gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc
    41701 tggcaacgtc tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc
    41761 tgctgtttgt cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta
    41821 aagtgatgcg taccaacgac cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg
    41881 aactggccgc gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc acccagggat
    41941 ttatcggtag cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata
    42001 cggcagcctt gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc
    42061 cgggcatcta caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg
    42121 cgtttgccga agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt
    42181 tgctacccgc agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg
    42241 caggtggtac gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc
    42301 ttcgtcgcaa tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct
    42361 tcctcgcgga agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca
    42421 cgtcagaagt gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata
    42481 cgttgctgac gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaggtggaag
    42541 aaggtctggc gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca
    42601 aagaggtatt cggcgtactg gaaccgttca acattcgcat gatttgttac ggcgcatcca
    42661 gccataacct gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg caaaaactgc
    42721 atagtaattt gtttgagtaa atactgtatg gcctggaagc tatatttcgg gccgtattga
    42781 ttttcttgtc actatgaggt agcctgagtt taacggacac tccttcctga aatagaatgg
    42841 catcagaagg agctaataat gagcagaaaa a
//
LOCUS       DAOFMX010000030        40606 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.030, whole genome shotgun sequence.
ACCESSION   DAOFMX010000030 DAOFMX010000000
VERSION     DAOFMX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.030"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..188)
                     /locus_tag="QBA20_001796"
     CDS             complement(<1..188)
                     /locus_tag="QBA20_001796"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HDQ6292361.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKG"
     gene            complement(329..2362)
                     /gene="metG"
                     /locus_tag="QBA20_001797"
     CDS             complement(329..2362)
                     /gene="metG"
                     /locus_tag="QBA20_001797"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDQ6292362.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2494..3603
                     /gene="apbC"
                     /locus_tag="QBA20_001798"
     CDS             2494..3603
                     /gene="apbC"
                     /locus_tag="QBA20_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDQ6292363.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3865..4146
                     /locus_tag="QBA20_001799"
     CDS             3865..4146
                     /locus_tag="QBA20_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDQ6292364.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4439..4963
                     /locus_tag="QBA20_001800"
     CDS             4439..4963
                     /locus_tag="QBA20_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6292365.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            5044..5718
                     /locus_tag="QBA20_001801"
     CDS             5044..5718
                     /locus_tag="QBA20_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDQ6292366.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5734..8214
                     /locus_tag="QBA20_001802"
     CDS             5734..8214
                     /locus_tag="QBA20_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ6292367.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKALGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8230..9263
                     /locus_tag="QBA20_001803"
                     /pseudo
     CDS             8230..9263
                     /locus_tag="QBA20_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9345..9683)
                     /gene="rcnB"
                     /locus_tag="QBA20_001804"
     CDS             complement(9345..9683)
                     /gene="rcnB"
                     /locus_tag="QBA20_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDQ6292368.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9902..10741)
                     /gene="rcnA"
                     /locus_tag="QBA20_001805"
                     /pseudo
     CDS             complement(9902..10741)
                     /gene="rcnA"
                     /locus_tag="QBA20_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            10863..11135
                     /gene="rcnR"
                     /locus_tag="QBA20_001806"
                     /pseudo
     CDS             10863..11135
                     /gene="rcnR"
                     /locus_tag="QBA20_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      11227..11323
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11358..12146
                     /gene="thiM"
                     /locus_tag="QBA20_001807"
     CDS             11358..12146
                     /gene="thiM"
                     /locus_tag="QBA20_001807"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDQ6292369.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12143..12943
                     /gene="thiD"
                     /locus_tag="QBA20_001808"
     CDS             12143..12943
                     /gene="thiD"
                     /locus_tag="QBA20_001808"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDQ6292370.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13008..13829
                     /locus_tag="QBA20_001809"
     CDS             13008..13829
                     /locus_tag="QBA20_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDQ6292371.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            13877..14623
                     /locus_tag="QBA20_001810"
     CDS             13877..14623
                     /locus_tag="QBA20_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDQ6292372.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14597..15562)
                     /locus_tag="QBA20_001811"
     CDS             complement(14597..15562)
                     /locus_tag="QBA20_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDQ6292373.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15559..16562)
                     /locus_tag="QBA20_001812"
                     /pseudo
     CDS             complement(15559..16562)
                     /locus_tag="QBA20_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16559..17836)
                     /locus_tag="QBA20_001813"
     CDS             complement(16559..17836)
                     /locus_tag="QBA20_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6292374.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18093..19145
                     /gene="fbaB"
                     /locus_tag="QBA20_001814"
     CDS             18093..19145
                     /gene="fbaB"
                     /locus_tag="QBA20_001814"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDQ6292375.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            19453..20307
                     /gene="gatY"
                     /locus_tag="QBA20_001815"
     CDS             19453..20307
                     /gene="gatY"
                     /locus_tag="QBA20_001815"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDQ6292376.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            20336..21598
                     /gene="gatZ"
                     /locus_tag="QBA20_001816"
     CDS             20336..21598
                     /gene="gatZ"
                     /locus_tag="QBA20_001816"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDQ6292377.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            21608..22060
                     /gene="gatA"
                     /locus_tag="QBA20_001817"
     CDS             21608..22060
                     /gene="gatA"
                     /locus_tag="QBA20_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDQ6292378.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            22091..22375
                     /gene="gatB"
                     /locus_tag="QBA20_001818"
     CDS             22091..22375
                     /gene="gatB"
                     /locus_tag="QBA20_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDQ6292379.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            22379..23734
                     /locus_tag="QBA20_001819"
     CDS             22379..23734
                     /locus_tag="QBA20_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDQ6292380.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            23782..24822
                     /gene="gatD"
                     /locus_tag="QBA20_001820"
     CDS             23782..24822
                     /gene="gatD"
                     /locus_tag="QBA20_001820"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDQ6292381.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24928..25701
                     /locus_tag="QBA20_001821"
     CDS             24928..25701
                     /locus_tag="QBA20_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ6292382.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(25783..26682)
                     /gene="yegS"
                     /locus_tag="QBA20_001822"
     CDS             complement(25783..26682)
                     /gene="yegS"
                     /locus_tag="QBA20_001822"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDQ6292383.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            27088..27405
                     /locus_tag="QBA20_001823"
     CDS             27088..27405
                     /locus_tag="QBA20_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292384.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(27747..29108)
                     /gene="yegQ"
                     /locus_tag="QBA20_001824"
     CDS             complement(27747..29108)
                     /gene="yegQ"
                     /locus_tag="QBA20_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDQ6292385.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29255..29586)
                     /locus_tag="QBA20_001825"
                     /pseudo
     CDS             complement(29255..29586)
                     /locus_tag="QBA20_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
     gene            complement(29777..30499)
                     /gene="baeR"
                     /locus_tag="QBA20_001826"
     CDS             complement(29777..30499)
                     /gene="baeR"
                     /locus_tag="QBA20_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDQ6292386.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(30496..31899)
                     /gene="baeS"
                     /locus_tag="QBA20_001827"
     CDS             complement(30496..31899)
                     /gene="baeS"
                     /locus_tag="QBA20_001827"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDQ6292387.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(31896..33311)
                     /locus_tag="QBA20_001828"
     CDS             complement(31896..33311)
                     /locus_tag="QBA20_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6292388.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(33312..36377)
                     /gene="mdtC"
                     /locus_tag="QBA20_001829"
     CDS             complement(33312..36377)
                     /gene="mdtC"
                     /locus_tag="QBA20_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDQ6292389.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(36378..39500)
                     /gene="mdtB"
                     /locus_tag="QBA20_001830"
                     /pseudo
     CDS             complement(36378..39500)
                     /gene="mdtB"
                     /locus_tag="QBA20_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(39500..40528)
                     /gene="mdtA"
                     /locus_tag="QBA20_001831"
                     /pseudo
     CDS             complement(39500..40528)
                     /gene="mdtA"
                     /locus_tag="QBA20_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
BASE COUNT        10232 a         9968 c        10496 g         9910 t
ORIGIN      
        1 cccttcttca ccttctccgc aatcagcttc agttccgctt tttccgccgc cgcagcatct
       61 gaaaaacttt ttatctgact ttgccctttg gtgccaactt ttccccaact gatatgcaac
      121 tcgtttccct gttgctccac cgtccagaat ttatgtgatt tttcgtcctg atagataaag
      181 tgtctcattc tgcatccctg taattttatc cagttgaatg cagatgctac cagtatttat
      241 acgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata aagaaaaggc
      301 gctgtcgatg cagcgccttg aagggggatt atttcacctg atgacccggt ttagcaccgg
      361 catccgggct tagcaggaaa atatctttcc cgccaggacc ggcagccatc accatgcctt
      421 cagagatacc gaagcgcatt ttacgtggtg ccaggttagc caccataatg gtgtgacgac
      481 caatcagtgc ctgcggatcc gggtaagcag aacgaatacc ggagaagaca ttgcgttttt
      541 caccgccgag atccagcgtc aggcgcagca gtttgtcaga accttcaaca aactctgcgt
      601 tttcgatcag cgccacgcgc aggtcaactt tggcgaagtc gtcaaaggtg atggtttcct
      661 gaatcggatc atctgccagt gggccagtaa ctggcgcggt agtggctttc acttcttctt
      721 tagaggcttc caccagagct tcaacctgtt tcatatcgat gcggttatac agcgccttga
      781 acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc agttccgtat
      841 tgaggaaggc ttctgcacgc tcggtcagtt tcggcagtac cggcttcagg taagtcatca
      901 gcacgcggaa caggttgatg cccattgagc aaatcgcctg caggtcagca tcgcgacctt
      961 cctgttttgc gactacccac ggagcctgct cgtcgacata gcggttagcc aggtcagcca
     1021 gtgccatgat ttcgcgcact gctttaccaa attcacggct ttcccacgct tcaccaatca
     1081 cttcagcggc atcggtaaag gttttgtaca gctcagggtc agccagctcg cttgccagca
     1141 caccgtcaaa acgcttgttg ataaagcccg cattacggga ggccaggtta accactttgt
     1201 tcacgatatc ggcattcaca cgctgaacga aatcttccag gttgagatcg atatcatcaa
     1261 tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca aaatgattca
     1321 gccaggtgct ggctttaata aaggtgccgc gagacttgga catcttcgcg ccgttcaccg
     1381 tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct tccagcatgg
     1441 caggccagaa caggctgtgg aagtaaacga tatctttacc gatgaagtgg tacagctcgg
     1501 cggtggagtc tttcttccag tattcatcga agcttacgct gtcgccgcgc ttgtcgcaca
     1561 gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag aaatatttgc
     1621 ccggtgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata tcccactgtt
     1681 gcaggccgga ttcaaaccac tcctgcattt tgtttgccac ctgctcctgc aacgcaccgc
     1741 tgcgggtcca tgcctgcagc atttcgctga aagagggcag atcaaagaag aagtgttcag
     1801 aatcacgcat cactggtgta gcaccagaaa ccaccgattt cggctcgatc aattcagtcg
     1861 ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg gatttacatt
     1921 tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc ggatcgtaca
     1981 gctgagagat ggtgcggttt ttaataaaac cgttttcttt caggcgagag tagataagtt
     2041 ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag ctgatgttaa
     2101 agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc tccggggtga
     2161 taccgagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg gcgcagatga
     2221 agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc tggatgtgct
     2281 ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac gtcaccagaa
     2341 ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa ggggttagat
     2401 agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt aaactatcag
     2461 ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca ggccaaatca
     2521 ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca cccaaccctg
     2581 aagcataacc tcaccacgct gaaagcgttg caccatgtcg cctggatgga cgacacgttg
     2641 catgtggaac tggttatgcc gtttgtctgg catagcgcgt tcgaagagtt aaaagagcaa
     2701 tgtagcgccg aactgctgcg catcaccggg gcgaaagcta tcgactggaa actgtcgcat
     2761 aacatagcca ctctgaaacg cgtgaagaat cagccgggaa ttaacggtgt gaagaacatt
     2821 atcgcggtga gttccggtaa aggcggcgtg ggtaaatcgt ccacggcggt aaacctggcg
     2881 ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat ctacggccca
     2941 tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga cggtacccac
     3001 atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct ggtcactgac
     3061 gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca gatgttgcag
     3121 gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg caccggtgac
     3181 attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt caccaccccg
     3241 caggacatcg cgttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa agtcgaggtt
     3301 ccggtgctcg gtattgtcga gaacatgagt gtgcatattt gcagtaactg tggtcaccat
     3361 gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca tacacagctg
     3421 ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg cacgccaacg
     3481 gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc tgaccgcgtt
     3541 gcagctcagc tctactggca gggtgaagtc attcctggcg agatttcctt ccgcgcggtc
     3601 taacgcctat taaccattct ggttattctt catacaccgc agagatgtta tattgatgcg
     3661 gtgtatttgt ttttgccgca ttaatgaaca cttatttaat actgcgaata ttctgcggac
     3721 cttcgtccga aactctacaa agtcgagtga tcgtacaggc aatgcttcat tatcatcctt
     3781 cgtgctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat ccaggcataa
     3841 cctttactac agggataacg ttcaatgaat aagtattggc tgtcaggaat tatttttctt
     3901 gcctatggat tggcttctcc cgcagtctct tcagagactg ccacattaac gattaatggc
     3961 aggatcagtc ccccaacatg cagtatggct atggttaata gtcaaccaca acagcattgt
     4021 ggtcagttaa cgtataatgt tgacacccgt catcaggttt cttcgcccgt caaaggtgtt
     4081 acgacagaag tggttgttgc aggtagcgat agcaaacgtc gaatcgtgtt aaatcgctac
     4141 gattaatttg gcaacctgat tttaattgca tttagaaaga cgaaatcatt tatataaatg
     4201 atttcgtctt ttttattgat atcaacaata ccatttacat aaatctacat taattaatag
     4261 atgtgataaa tatttaatat tcttaagtat ttgttgtatt tatattgcat tggttaattt
     4321 gttttaaatc aataaaacaa tatgtttaca ttgcggcagc ttttttgatg tgagaaattt
     4381 gtttccaaga gatacagact cttaacaacc aaaaataaat tttattaagg aacatattat
     4441 gaaacgttca attattgctg ccgctgtctt ttcttctttt tttatgagcg ctggagtatt
     4501 tgctgcagac gttgataccg gaacattaac cattaaagga aatattgcag aatctccgtg
     4561 taaattcgaa gcgggtggtg attcagtaag tattaatatg ccgactgtac caaccactgt
     4621 ctttgaaggt aaagctaaat attctaccta tgatgatgca gtcggtgtaa ccagcagcat
     4681 gttaaaaatt agctgcccga aagaagttgc tggtgtaaaa ctctcgttga tcacaaacga
     4741 caaaataacc ggtaacgata aggcgatagc cagtagcaac gataccgtag gtgataacag
     4801 cgatgtccta gatgtttctg caccttttaa cattgagagt tataaaacag cggaaggtca
     4861 atatgctatt ccgtttaaag caaaatacct gaaactgaca gataactcag tgcaatcagg
     4921 tgatgtgtta tcttctctgg ttatgcgtgt ggcgcaggat taataaataa aattgcaagt
     4981 ttctccaggt aataaacatg gccgctatcc ccatggcggc tttttaattt aagaggcatt
     5041 aagatgaaag gattattatc tttactcatt ttttctatgg tccttcctgc acatgccgga
     5101 attgttatct acgggacgcg cattatttac ccggcagaaa ataaagaagt tatggtgcag
     5161 ttgatgaacc agggaaatcg ttcttcgctg ctgcaggcgt ggattgatga tggcgatacg
     5221 tcgttaccac cagaaaaaat tcaggttcct ttcatgttaa cgccaccagt ggcaaaaata
     5281 ggggcaaatt ccgggcagca agtaaaaatc aaaattatgc cgaataaact gcccactaat
     5341 aaagaaagca ttttttatct gaatgttctg tatattccac caaatagtcc agagcaagaa
     5401 ggcaagaatg cactgaagtt tgcgatgcaa aacagaatta agttgtttta ccggccagcg
     5461 ggtattgctc cggtaaataa agcgaccttt aaaaagttac tggtaaatcg cagtggcaat
     5521 ggattggtga taaaaaatga ctcagctaat tgggtgacga tttcggatgt caaagctaat
     5581 aatgttaaag tcaattatga aactattatg attgccccct tagaaagtca gagtgttaat
     5641 atcaaaagta ataatgcaaa taactggtat ctgaccatta tcaatgacca cggcaactat
     5701 attagtgaca aaatttaatt gcaggagctg cctatgttga gaatgacccc acttgcatca
     5761 gcaatcgtag cgttattgct cggcattgaa gcttatgcag ctgaagaaac ctttgatacc
     5821 cattttatga taggtggaat gaaagaccag caggttgcaa atattcgtct tgatgataat
     5881 caacccttac cggggcagta tgacatcgat atttatgtca ataagcaatg gcgcgggaaa
     5941 tatgagatta ttgttaaaga caacccgcaa gaaacatgtt tatcaagaga agttatcaag
     6001 cggttaggca ttaatagcga taacttcgcc agcggtaagc aatgtttaac atttgagcaa
     6061 cttgttcagg gtgggagcta tagctgggat atcggggttt ttcgtctcga tttcagtgtc
     6121 ccgcaggctt gggtggaaga actggaaagt ggctatgttc caccggaaaa ctgggagcgg
     6181 ggtattaatg cgttttatac ttcttattat gtgagtcagt attacagcga ctataaagcg
     6241 ttgggtaaca acaagagtac atatgtacgt tttaacagcg ggttaaattt actggagtgg
     6301 caactgcatt ctgatgccag tttcagtaaa acaaataaca atccaggggt gtggaaaagc
     6361 aataccctgt atctggaacg tggatttgcc caatttctcg gcacgcttcg cgtgggtgat
     6421 atgtacacat caagcgatat ttttgattct gttcgcttca gcggtgtgcg gttgtttcgt
     6481 gatatgcaga tgttgcctaa ctcgaaacaa aattttacgc cacgggtgca ggggattgct
     6541 cagagtaacg cgctggtaac tattgaacag aatggttttg tggtttatca gaaagaggtt
     6601 cctcctggcc cgtttgcgat tacagatttg cagttggccg gtggtggagc agatcttgat
     6661 gttagcgtga aagaggcgga cggctcggta accacctatc tggtgcctta tgcagcggtg
     6721 ccaaatatgc tgcaacccgg cgtgtcgaaa tatgattttg cggcgggccg tagccatatt
     6781 gaaggggcga gcaagcaaag tgattttgtc caggcaggtt atcagtatgg ttttaataac
     6841 ttattgacgc tgtatggcgg cacgatggtt gctaataatt actatgcgtt taccctcgga
     6901 acgggttgga acacgcgcat tggcgcaatt tccgtcgatg ccacgaagtc gcatagtaaa
     6961 caagacaacg gcgatgtgtt tgacgggcaa agttatcaaa ttgcctacaa caaatttgtg
     7021 agccaaacgt cgacgcgttt tggtctggcg gcctggcgtt attcgtcgcg tgattaccgg
     7081 acatttaacg atcacgtttg ggcaaacaat aaagataatt atcgccgtga tgaaaacgat
     7141 atctatgaca ttgccgatta ttaccagaac gattttggcc gtaaaaatag cttttctgcc
     7201 aatatgagcc agtcattgcc agaaggctgg ggttctgtgt ccttaagtac gttatggcga
     7261 gattactggg ggcgtagcgg cagcagtaag gattatcagt tgagttattc caataactgg
     7321 cggcggataa gctataccct cgcggcaagc caggcgtatg gcgagaatca tcatgaagag
     7381 aaacgtttta atatttttat atcaattccc tgtgattggg gtgatgacgt tacgacgcct
     7441 cgtcggcaaa tatatatgtc taactcaacg acgtttgatg atcagggttt tgcctcaaat
     7501 aatacgggat tatcaggaac agtagggagt cgggatcagt tcaattatgg tgtcaacctg
     7561 agtcatcaac atcagggaaa tgaaacgaca gctggggcga atttgacctg gaacgcgccg
     7621 gttgcgacag tgaatggcag ttatagtcag tcgagtactt atcgacagac tggagccagt
     7681 gtttcagggg gcattgtcgc ctggtcgggt ggcgttaatc tggcgaaccg tctttccgaa
     7741 acgtttgctg tgatgaatgc gccaggaatt aaagatgctt atgtcaatgg gcaaaaatat
     7801 cgcacaacaa accgtaatgg agtggtggta tacgacggaa tgacacctta tcgggaaaat
     7861 cacctgatgc tggatgtgtc gcaaagcgat agcgaagcag aattacgtgg caaccggaaa
     7921 attgccgccc cttatcgcgg cgcggttgta ctggttaatt ttgataccga tcagcgcaag
     7981 ccctggttta taaaagcgtt aagagcggat ggtcaaccat taacgtttgg ttatgaagtc
     8041 aatgatatcc atggtcataa tattggcgtt gtcggccagg gaagccagtt atttattcgc
     8101 accaatgaaa taccgccatc ggttaatgta gcaattgata agcaacaagg actttcatgc
     8161 acaatcacct tcggtaaaga gattgatgaa agtagaaatt atatttgcca gtaatagtca
     8221 ggtggttcta tggaaattcg cataatgcta tttatattaa tgatgatggt tatgcctgtg
     8281 agctatgcgg catgttatag tgagttatct gttcagcaca atttggttgt tcaggggaat
     8341 tttgcactta ctcaaacaca aatagcgaca tatgagcata attttaatga ttcgtcatgc
     8401 gtgagtacaa atactatcac ccctatgagc ccatcggata ttattgttgg actttataac
     8461 gataccatta aattaaattt acattttgaa tggaccaata aaaacaacat cacgttgtca
     8521 aataatcaga gcagtttcac cagtggttat tcggtaacgg tgacacctgc ggccagtaat
     8581 gcaaaagtga atatttctgc ggggggcggt ggttcagtga tgattaatgg tgttgcgaca
     8641 ttatccagtg cttcatcatc gacacgcggg agtgccgcag tacaatttct actgtgttta
     8701 ttaggtggca agtcatgggg tgcatgtgta aatagctaca gaaatgcatt ggcacaaaat
     8761 gcaggtgtct attccttcaa tctgacattg tcatacaacc cgataaccac aacctgcaaa
     8821 caggacgatt tattaattac tttagacagt attcccgttt cacaattacc agccacaggt
     8881 aacaaagcaa caataaatag taaaaaaagg gatattattc tgcgttgtaa aaatttatta
     8941 ggtcaacaaa atcaaacatc acggaaaatg caggtgtatt tatcaagttc tgacttgtta
     9001 accaacagca acacaatact gaaaggtgcg gaagataatg cgtaggattt attcttgaaa
     9061 gtaatggttc gccagtcaca cttttaaata tcactaacag cagtaaagga tacacaaatt
     9121 taaaagaaat tgcggcaaag tcaaaactta cagatacaac ggtttcaatt ccgataacag
     9181 ccagttacta cgtctacgat acaaacaaag ttaaatctgg cgcactggag gcaaccgcat
     9241 taatcaacgt gaaatacgac taataacaag caaaacgagc aagtggctaa taataaaaaa
     9301 taacaaggtg tctgcatttc cccctcatga tgagggggct ttttttagcg atgataaaaa
     9361 atctcaccgt cgtaggcttt aatgatttta ccgtcggtgt cgctgatcaa cacgtacgcg
     9421 ccacccatat aggtccagtg cgtcccggca tcaggcgcgg gcaggttacg taactgccac
     9481 tgcttaatgt tgtactcatc ggtgcggtac atctctggga cggtatcacc tggtttgaaa
     9541 tgcttaaagt cagcaatgaa actacttaac tcatacttag aaattcccga ggtattggtc
     9601 gaacccgcgg tggctggtgc ggcccaggcg gcactggtaa ccagcaaaag cacacccagc
     9661 aacatcttat ttttaatggt cataaactct ccactttggg gatctgtaat atcagtcgca
     9721 tatctttcct gtcatacaat ataactgaca agcgatattt tgttagtgat cccatgattt
     9781 cgtaaaaagt gtaaatgaaa ggatgcccta aaagcatcct tagcgagcag agagtcagcg
     9841 gaaatagctg atttaagaat catcctgggc tgaagataac atgcaagcct aaaggattga
     9901 gttatcgcat tatgcccatg aagccatgta cgcccatata cacaccgact aagccaatca
     9961 acagactgga aaaatagggg gcgcgtttag cgagagtgtt aaatccgctc cagcgttttg
    10021 cgacctgctg aacgctgatt gctgcgccaa cgcctacggt gacaagcgtt aacgccaggc
    10081 caatgctgaa actgacgacc agtgttgcgc ccagtgtcag ggctttcaac tgaatgcata
    10141 tcaacagcac ggtaattgct gccgggcagg ggataaagcc accggttaag ccaaataaca
    10201 aaatttgcca gttgatgacc tctctaccat caaagcgtcg tttaatgtca ttggcatggg
    10261 ctcgtgcatg ggcatcctga tactcgccat gttcgtgatg gtgatgatgt ccttggtcgt
    10321 ggtgatgttc gtggtcttga tgatgttcgt gatcgtgatg atgctcataa tcatgcccgt
    10381 gcatattctc cagccagttg cgttcgccgc gctaggtacg ccagaacatc cagaacgcgg
    10441 tgccaataat gatcactgcg gaaatcagct ggagccacgg ttctgctgat tgagcagtaa
    10501 agcgcttact gatcaccatc ccgccaaagg caatcaacca gaccaccgcg gtatgcgaaa
    10561 tggttgctgc cagtccgagc atcaccgcct gtttaatggt gcctttgatg gcgatgataa
    10621 acgccgccat catcgttttt gagtgccctg gttccaggcc atgaagcgca ccaagtaaga
    10681 tggcgctggg gatgaagaac caggcgtttc cttgctgaag aagagttgta aattcggtca
    10741 tgataataat tcttagtatt aattcggcaa tctgattcta ctccccccca gtacctgata
    10801 ctaccccccc agtagattaa tagtgctatg atttttcata ttcttgttaa ccaggtgttg
    10861 ccatgtctca tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg
    10921 gccaggtcgt ggcgctcaag aaaatgctcg acgagccgca cgaatgcgct gcagttttac
    10981 aacagattgc tgctatccgt ggagcggtaa acggtctgat gcgggaagtg attaaaggtc
    11041 atctgacgga acacatcgtt cactaggggg atgagctaaa acgtgaagaa gatctggatg
    11101 tcgttctgaa ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc
    11161 cgtttttcct cgtttacaac gggtgcgctg gacattacca tcctcctctg cgatttatca
    11221 tcgcaaccaa acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc
    11281 tgatctggat aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt
    11341 tatggcagga gcaaactatg caagtcgacc tgctgagttc agcgcaatct gcgcacgcgt
    11401 tacacctttt tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa
    11461 cctttaccgc caataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg
    11521 aagaggccag tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga
    11581 cgcagccacg cgcacaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac
    11641 gctggacgct tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg
    11701 aacttttatc ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag
    11761 ctggcattgc taatggcgga cggggagtgg ataccactga cgccgcagct aacgcgatac
    11821 ccgctgcaca aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg
    11881 attatgttac cgatggacat cgtatcattg gtattcacgg tggcgatccg ttaatgacca
    11941 aagtggtagg aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag
    12001 gcgatacgct ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagaacgcg
    12061 cagtcgccag aagcgagggg ccaggcagtt ttgttccaca tttccttgat gcgctctggc
    12121 aattgacgca ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga
    12181 tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta
    12241 tggttgctca gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta
    12301 tcgcattgag cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat
    12361 cgataccact aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg
    12421 gttgcaacgt tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg
    12481 cgacccgctg ctttcacctt cggcggttgc tacgctgcgc agtcgattat tgccacaggt
    12541 ttcattaata acgccaaact tgcccgaagc tgccgccttg ctcgacgcgc cacacgcgcg
    12601 caccgaacag gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt
    12661 gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga
    12721 gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa aacacccacg gcactggttg
    12781 tacactctct gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt
    12841 acaggaggca aaaatctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg
    12901 tcacggtatt ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg
    12961 ctttggttat tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa
    13021 ttaccagtcg aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg
    13081 caatttatcc gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact
    13141 acatccactt ctacggttat cgcccggtta aatctttcgc tattcgcatt cctgccagtt
    13201 acaccattca cggattagat gtttcgcgct ggcaggagcg tatcgactgg cagcgggtgg
    13261 caaaaatgcg cgacaacgat atccgcttac agtttgcttt tattaaggct acggaaggcg
    13321 aaaagctggt ggacccctat ttttcgcgta actggcaatt aagccgcgaa aatggcctgc
    13381 tgcgcggggc gtatcattat ttctccccgt cggtatccgc ttcagttcag gcgcgattat
    13441 ttctgcaaac ggtggatttc tcacaaggcg atttccctgc cgtgctggac gtagaagaac
    13501 ggggaaaatt atcggcaaaa gaattacgca agcgggtaag tcagtggcta aaaatggtcg
    13561 aaaaacgcac gggaaaaaag ccgattattt actcaggagc cgttttttat cacaccaatc
    13621 tggcgggcta tttcaatgaa tatccgtggt gggtggctca ctattatcaa cgtcgtccgg
    13681 acaatgacgg catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca
    13741 tcaatggtcc ggtggatttt aatgtgttta atggcacggg gatgagttgc aggcattcgt
    13801 tgatgggatt aaagaaacgc cttaaataag aagcagtgat atactcccac cagactccct
    13861 ggcaggaaaa agacaaatgg aacaagcgca tacccagctg attgcccaac tgaacgagcg
    13921 tattttagcg gcggataaca cgccgcttta tattaagttt gccgaaacgg taaaaaatgc
    13981 cgtgcgcagc ggggtgctgg agcatggcaa tattttgccc ggtgagcgtg atttaagtca
    14041 gttaaccggc gtttcgcgca ttacggtgcg caaggcgatg caggcgctgg aagaagaggg
    14101 cgtggtgacg cgttcgcgcg gttacggcac gcagatcaac aacatcttcg aatactcgtt
    14161 gaaagaagcg agaggttttt ctcagcaggt ggtattgcgc gggaaaaagc ccgatacgct
    14221 atgggttaat aagcgtgtcg tgaaatgtcc cgaagaagtt gcgcagcagc tggcggtcga
    14281 agcgggaagt gatgtctttt tgcttaagcg tattcgctat gtcgatgaag aagcggtatc
    14341 gattgaggaa tcgtgggttc cggcgcattt aattcatgat gttgatgcca tcggaatttc
    14401 tctttatgac tatttccgca gccagcacat ttacccacaa cgtacgcgtt cccgcgttag
    14461 cgcccggatg ccggatgccg agtttcagtc acatattcag ttagatagca aaataccggt
    14521 gctggtgatc aagcaagtgg cgcttgacca acagcaacgg cctattgagt acagcattag
    14581 ccactgtcgc agcgatctat acgtttttgt gtgcgaggag tagttcctcg cgcgttggcg
    14641 cacaatcacc gccccgatgc ccgacaaccc acgacgccac tgcattgccc agtaatacgg
    14701 catccgccag tggcagacca gaggccagcc cggcaagtac gccacctgca tgactgtcgc
    14761 ccgccccaat ggtgtctaca acttgcgttg gaaatgccgg aatgcagcca gaagcgtcgt
    14821 tgctgaaata ccatgcgcct tctttatcga gacgaacgat caacggcgcg gcaaatttct
    14881 cctgccattg cttgccaagt gttgttatct ctgcggataa agcaaaacgt tcggcggcaa
    14941 tctcagcctc ttgacgattg agtgacacta aaggtcgaca ggccatgatc cgcgccagta
    15001 atgcgtccgg gatatcgcca atacgtgggc aaaaatcgat aaacggcgtc acgtcttgca
    15061 gcttttccag ccattccact aacaattcgc cgcagggcga ggccagttga taaccggaaa
    15121 aatagagcag gttgccaggc gcaacggtta atcgtgccaa ccattggcga ttccattgat
    15181 tttccacacc gctgaatgac ataaaagtgc gttcgccatc cggctcaacc agtgccagac
    15241 accagccgtt atcgctttcg gcgttatcga tcaggctgat taacccctct ttcgccatcc
    15301 ggttgcgaat aatctccgcc cacacgccct gaccgagcgg caaggcatta cccgcttcga
    15361 tgccgaggcg ctttaacgcc acggcaatat tcagtgcgca gccgccaacg ttaacgctct
    15421 gctgtttcag ttcgatatcg cacccacgcc aggggagggc ataagcgtcg gcgatcacgt
    15481 caatgaccgc cgcgccgacg accatcaccg gctgacgcgt ggttaattca ggcagcagtg
    15541 tgtgtaatcg agcgccgctc ataccgcctc ccgttgttga cgatatttcg ccagcgctgt
    15601 ggcatagcgg ttgaaatcaa gctgatttac cgcatccagc tccgccttta acgcaggatc
    15661 gacagcgtta atgccatgca acgcgccgca aatcgccgtc gccatagcac ctatggtgtc
    15721 tgtgtcgccg ccaagattgg cgcacaggac ggcgcagcgg tttgggtcgg tttgtgccag
    15781 ttcaactagc gcaatggcgc acggaacgga ctcaatagtg ctggtgcctg cgccaacgac
    15841 ctggtaaagc tgttcgctgg cggattcggt gccgtcggca ttacgcacaa ttttcagcgc
    15901 aatctccaga cgtgccgcca gtgaggcgct gaacgtggtg gtgcgttttt gctgtgcatg
    15961 tcgcgcaatt gaaggcaatg aatcaacaat cgctgaccag cgttctccgt caatggcacg
    16021 agaaatcgcc catgcgatga ctaccgcgcc tgcaaccgcc agatcggatt tatgggtcgg
    16081 gctggacgcc agcgccacat catcaataaa ggaatcaaca tcacgcgctg gaagcaaaca
    16141 acctaatggc gagacgcgca tcgctgcgcc gttggttacg ccgttgtttt ccagttcagc
    16201 aacgggttta ccgtcgcgaa tggcgttaag cgcaatcttc gaggtcggac ctagtacgtt
    16261 tttgttaaag gcgtcgaaac gcaacgccca gtcgagaata ttacgcccaa tcagattcgg
    16321 atcgatcttg ccttcacatt ccagtaacgc atccgccaga cacaggccat cgaggtatcg
    16381 tcggtgaatt cggcgcggtt aaaataacag gccgcgttat tctcctttgg tccaggcaga
    16441 aaacggtcaa tccagccaaa gtgtgcttta acgcggctgc gtggccaaag ctcggagggc
    16501 atccccatcg catcccctaa cgcctgccca taaagagcac cgagaatacg ttctgttttc
    16561 atttaacttc cccttgtgtc aacgcaatat ctcgatcatc gaccttgata gccgtaattt
    16621 cgttgtcgga ttcgcggaaa aaaatcatga acagcacggc gataatggca atcatcaccg
    16681 cgccgaaagt ccacatcccg gaccagttga aagtcagtcc gtttaccggt tcctgataag
    16741 cgaacatctt ttccatcatc acaccgccaa gacgatagcc gagcaaactg ccgaagccct
    16801 ggcagcagag cgtgatcagc ccctgcgcag cggtacgcat atgcacgggg gcttttttat
    16861 cgacatagat gtaagcggta acgtagtaaa aatcgtaact tacgccgtga agcaaaatac
    16921 cgaggaacag taacgcgtag gtgaaatatt catccgcact accgtaaata aagaagccat
    16981 agcagatcgc agcggtgacc agaccaagca ataatacctt tttgatacca aagcgtttag
    17041 tgaaaaacgg caatgccagc ataaagaaga tttcagagaa ctggccgagc gtcatccagc
    17101 cggtggcgtt tttcatgcca acttcggtca gataaccgtt ggcaaagatg taatagaacg
    17161 ccagtggcat cgcaaacagg aatgaacaga agaaaaagac gaggaagttt ttatcgcgca
    17221 gcaggatcag cgcatccagg ccgagcatga ctttaatgtc cattttgccg gtgctttttg
    17281 gcggcgtgtc gggcaggaaa aacgcaaaca caccgagcag agcagaactt ccggcggtaa
    17341 tcagcagcgg gatgttagtc ggtgagatat cggcataccc cagcatttgc ggcaagaaac
    17401 cacatgccag accggaggca atccagccga tagtgcccat cacacgaatg cgtgggaaat
    17461 cacgctccac atccggcacg ttggcaaaag cgatgctgtt agtcagcgca atggtcggca
    17521 tataggttag cgagtaggcc agcagtaacg gaaagaaccc ggcaaaagtg gtctgttgcg
    17581 cagcgaaata catcagcaca gcaccagcga acatcaatac cgccagcact ttctgcgccg
    17641 agaaaaagcg gtcagtgatg gagccaacca gaattggcga caggatcgcc gcaatggctg
    17701 tgcaggcata cgaccagcca atttctccgg cactaaaacc gcttttactt aaccacaacc
    17761 acaatggcac aaaccacgcg ccccagataa accattcaac aaacatcatg aacgacagct
    17821 ttgctgttgt tttcatcttt taattccttc atgacaggta atgtaaggta cgcgatgaca
    17881 ataccacata ataatgcctt ttgaatacct ttgcggtgaa aacttgaccg cgctaacatt
    17941 ttttctgatg aatcgagcca acagaaaacg ctgaaaaaac atccaaaaga tggaaaaact
    18001 cgcccgtaac gactaggatt atgattgccc gcaaaaggcg ggccaggaca atccatagcc
    18061 gatatccaat cggaatttac gggagcatag taatgacaga tattgcgcag ttgcttggca
    18121 aagacgccga caacctttta cagcaccgtt gtatgactat tccttctgac cagctttatc
    18181 tccccggaca tgactacgta gaccgcgtga tgattgacaa taatcgcccg ccagcggtgt
    18241 tacgtaatat gcagacgttg tacaacaccg ggcgtctggc tggcacagga tatctttcta
    18301 ttctgccggt tgaccagggc gttgagcact ctgccggagc ttcatttgct gctaacccgc
    18361 tctactttga cccgaaaaac attgttgaac tggcgatcga agcgggctgt aactgtgtgg
    18421 catcaactta cggcgtgttg gtgtcggtat cgcggcgcta tgcgcatcgc attccatttc
    18481 tcgtcaaact taatcacaac gagacgctaa gttacccgaa tacctacgat caaacgctgt
    18541 atgccagcgt ggagcaggcc ttcaacatgg gcgcggtggc ggttggtgcg actatctatt
    18601 ttggctcgga agagtcacgt cgccagattg aagaaatttc tgcggccttt gaacgcgcgc
    18661 acgagctggg catggtgaca gtgctgtggg cctatttgcg aaactccgcc tttaagaaag
    18721 atggcgttga ttaccatgtt tccgccgacc tgactggtca ggcgaaccat ctggcggcaa
    18781 ccataggtgc agatatcgtc aaacaaaaaa tggcggaaaa taacggcggc tataaagcga
    18841 ttaattacgg ttataccgac gatcgcgtgt acagcaagtt aaccagcgaa aacccgattg
    18901 atctggtgcg ttatcagtta gctaactgct atatgggccg ggctgggttg ataaactccg
    18961 gcggtgctgc aggcggtgaa actgacctca gcgatgcagt gcgtactgcg gttatcaaca
    19021 aacgcgcagg cggaatgggg ctgattcttg gacgtaaggc gttcaagaaa tcgatggctg
    19081 acggcgtgaa actgattaat gccgtgcagg acgtttatct cgatagcaaa attactattg
    19141 cctgatacgc ctcatacgaa cagcaccacc ccgtaggacg gataagacgt ttacactgca
    19201 tccggcaacc tatgcctgat gcgacgcgga agcgtcttat catgcctaca tagcactgcc
    19261 acgtatgttt acaccgcatc cggcataaaa acacgcgcac tttgctacgg cttccctatc
    19321 gggaggccgt ttttttgcct ttcactcctc gaacaatttt catattgttg tttttgtgat
    19381 cattatctcg acatttaaaa gcaaataatt tcattatatt ttgaaatcga aagcaaacga
    19441 caagatatga aaatgtacgt ggtatcgaca aagcagatgc tgaacaacgc acagcgcggc
    19501 ggttatgcgg ttccggcatt caatattcac aatctcgaaa cgatgcaagt ggtggtggaa
    19561 accgctgcca acctgcatgc gccggtcatc atcgccggaa cgcctggcac atttactcat
    19621 gctggtacag aaaatctgtt agcgctggtc agcgcgatgg cgaagcaata tcaccatcca
    19681 ctggcaattc atctcgacca tcacacgaaa tttgacgata tcgctcagaa ggttcgttct
    19741 ggcgtgcgct cagtcatgat tgacgcctcg catttgcctt ttgcgcaaaa tatttcacgg
    19801 gtcaaagagg tggtggattt ttgccatcgc tttgatgtca gcgtcgaggc ggagctgggg
    19861 caacttggcg gccaggaaga tgatgtgcaa gtcaatgaag tcgatgcgtt gtacaccaac
    19921 cccgcacagg cgcgtgaatt tgccgaggca accgggattg attccctggc ggtcgccatc
    19981 ggcacggctc acggtatgta tgccagcgca ccggtgcttg atttttctcg actggagaac
    20041 attcgccagt gggtgaactt accgctggtg ctgcatggcg cgtcaggatt atcgactaag
    20101 gatattcagc aaaccatcaa actggggata tgcaaaatca acgttgcaac ggagctgaaa
    20161 aatgccttct tgcagtcgtt aaaaaattac ctgaccgagc accccgaagc gaccgatccc
    20221 cgggattatt tgcagtcggc taaatccgca atgcgcgatg tggtgagcaa agtgattgcc
    20281 gattgtggct gcgagggcag ggcataacgc acctgccatt taacaaggaa aaaacatgaa
    20341 aacgttaatt gcccggcata aagctggtga acatatcggc atatgttcag tctgttctgc
    20401 ccatccgttg gttatcgaag cggcgctggc atttgatcgc aacagcacgc gcaaagtgct
    20461 gattgaagca acgtcaaacc aggtcaatca atttggcggt tataccggaa tgacaccggc
    20521 agactttcgc gaatttgttt ttacgattgc cgataaagtt gggtttgcac gcgaacgcat
    20581 tattctcggc ggcgatcatc tgggaccaaa ctgctggcag caagaaaatg cggatgcggc
    20641 gatggaaaaa tccgtcgagc tggtaaaagc atatgttcgt gctggcttca gtaaaattca
    20701 tcttgatgcg tcaatgtcct gcgcggggga taccataccg ttagcaccag aaacggttgc
    20761 ggaacgagct gctgtgcttt gctttgctgc ggaaagtgtg gcgacagatt gccagcgtga
    20821 gcaactgagc tatgtcattg gcactgaagt tccggttccg ggcggtgagg ccagcgccat
    20881 tcagtcagta cacatcaccc atgttgaaga tgccgccaat actttacgta cgcatcaaaa
    20941 ggcctttatt gcccgtgggc tgacagaggc gttaacacgt gtgattgcca tcgtggtgca
    21001 gccgggtgtg gaatttgatc acagcaatat tatccattat cagccgcagg aagcgcaggc
    21061 gctggcgcaa tggatagaaa acacccgaat ggtttatgaa gcacattcta ccgattacca
    21121 gacccggacg gcttattggg aattagtccg cgatcacttt gcaatattga aagtcggtcc
    21181 cgcattaacc tttgctttac gcgaggcgat atttgcactg gcacaaattg agcaggaact
    21241 tatcgccccc gaaaatcgca gcggttgcct ggcggtaatt gaagaagtga tgctcgacga
    21301 accgcaatac tggaaaaaat attatcgcac gggttttaac gattcattac tggatattcg
    21361 ttacagcctg tcggatcgta ttcgttatta ctggccgcat agtcggatta aaaatagcgt
    21421 tgaaacgatg atggtgaatc tgcaaggcgt ggacatccca ctgggcatga ttagtcagta
    21481 tcttcccaaa caatttgaac gcattcagtc cggggaatta tcagcaatgc cgcatcagct
    21541 tattatgaat aaaatttatg atgttttgcg cgcctatcgc tacggctgtg cggaataagg
    21601 acggtatatg actaacctgt ttgttcgtag cggaatttct tttgtcgatc gtagcgaagt
    21661 tttaacctat atcggtaatg agatgctcgc gaaaggtgtg gttcatgata cctggccaca
    21721 ggcattaatt gccagagaag cagaattccc taccgggata atgcttgagc agcacgccat
    21781 tgcaataccg cattgtgagg cgattcatgc taagtcgtca gccatttatc tgttaaggcc
    21841 aacaaataaa gttcattttc agcaagcgga tgatgataac gacgtggcgg tatcgttggt
    21901 tattgcgttg attgtggaaa atccgcagca gcaattgaaa cttttacgct gtttatttgg
    21961 caagttacaa cagcccgata tcgtcgagac actaattact cttcctgaaa ctcagttaaa
    22021 ggaatacttc acaaagtatg ttttagattc agacgaataa atccctctgt aacaataaaa
    22081 aggactattt atgaaacgca agattattgt cgcttgcgga ggcgcggttg cgacctctac
    22141 gatggcggcg gaagaaatta aagagttgtg tcagagtcat aatattcctg ttgaattaat
    22201 ccagtgtcgg gttaatgaaa tagaaaccta tatggatggt gtgcatttga tatgtaccac
    22261 tgccagggtg gatcgtagtt ttggcgatat tccgttagtt cacggcatgc cttttgtttc
    22321 tggtgtcggt atcgaagcat tacaaaataa aattctgact atcttacagg ggtgacctat
    22381 gttttcagaa gtcatgcgtt atattctcga cctcggccct acagtgatgc tgccggttgt
    22441 catcattatt ttttctaaaa tattaggcat gaaggcaggc gattgcttta aagcgggtct
    22501 gcatatcggg attggctttg ttggcattgg ccttgtgatt ggcttaatgc tggattccat
    22561 tggtccggcg gcgaaagcga tggcggaaaa tttcgacctg aatctgcatg tggtcgatgt
    22621 cggctggccg ggctcttcac caatgacctg ggcgtcgcaa attgcgctgg tggcgattcc
    22681 gattgcgata ttggttaacg tggcgatgtt actgacccgt atgacgcggg tggtaaatgt
    22741 tgatatctgg aatatctggc atatgacctt caccggcgcg ttgctgcacc tggcaaccgg
    22801 ttcatggatg atagggatgg cgggtgtggt aattcacgcg gcgtttgttt ataagctctg
    22861 cgactggttt gcccgcgata cccgaaattt ctttgagctg gaaggtattg ctattccgca
    22921 cggtacgtcg gcgtatatgg ggccgattgc ggtgctggtc gatgctatca tcgagaaaat
    22981 cccaggcgtt aaccgaatta aatttagcgc cgacgatatt cagcgcaaat ttggcccttt
    23041 tggcgaacct gtcaccgtgg gttttgtgat ggggctgatt atcggcatcc tcgcgggtta
    23101 cgatgtcaaa ggtgtattgc agctggcggt aaaaacggcg gcagttatgc tgctgatgcc
    23161 acggatgatt aaacccatca tggatggttt aacgcccatc gctaagcagg cccgtagtcg
    23221 tttacaggcg aagttcggcg gtcaggagtt cctgattggc ctggatcccg cattactgct
    23281 ggggcatacg gcggtggtat cggcaagcct gatttttatt ccgctcacca ttttaattgc
    23341 tgtttgtgtt ccgggtaatc aggtactgcc gtttggcgat cttgccacta tcggcttctt
    23401 cgtggcgatg gcggtcgcgg tgcatcgtgg aaatctgttc cgcaccttaa tctcgggtgt
    23461 catcattatg agcatcaccc tgtggatcgc gacgcaaact attggtttgc acacccaacc
    23521 ggcggctaat gctggggcgt taaaagccgg gggtatggtg gcttcaatgg atcagggcgg
    23581 ttctcccatt acctggttac tgattcaggt tttctccccg caaaatattc ccggtttcat
    23641 tattatcggc gcaatttatc tgaccggtat tttcatgacc tggcgtagag cgcgtggttt
    23701 tactaaacaa gagaaagccg ttctcgcaga ataattttta cctgaggggg aggttatccc
    23761 ccttaacaat caggagtttt tatgaaatca gtagtgaatg atactgatgg tatcgtgcgc
    23821 gttgcaaaaa gcgtcattcc tgaaattaaa catcaggatg aggtgcgggt aaaaattgcc
    23881 agctcgggct tatgtggttc cgatttaccc aggatattta aaaatggtgc acattattat
    23941 ccaataacgt taggccatga atttagcggc tatattgatg cggtgggatc cggtgttgat
    24001 gatttacacc ctggcgatgc ggttgcctgt gtgccgttat taccctgttt tacttgtcca
    24061 gagtgtttga aagggtttta ttcccagtgc gcaaaatatg attttattgg ctcgcggcgt
    24121 gatggtggat ttgctgaata tattgtcgtt aagcgaaaaa atgtctttgc tctacccacg
    24181 gatatgccta ttgaggatgg ggcttttatt gagccgatta ccgttggcct gcatgctttt
    24241 catttagcgc aaggttgtga gaataaaaac gttattatta ttggtgccgg aaccattggc
    24301 ctgctggcta ttcagtgcgc tgtcgcgctg ggagcaaaga gtgtgacggc gatcgacatt
    24361 agctcagaaa aactggcact ggcaaaatct ttcggtgcga tgcaaacatt taacagtagc
    24421 gaaatgagcg cgccgcaaat gcagagcgtt ttacgcgaac tgcgctttaa tcagcttatc
    24481 ctcgagacgg ctggcgtacc gcaaactgtc gaactggcgg tagagattgc cggtcctcat
    24541 gcccaactgg cgctggtggg cacgttgcat caggatctgc atttaacatc ggcaacgttt
    24601 ggcaaaatat tgcgtaaaga gctgacggtt atcggcagtt ggatgaacta ttccagccct
    24661 tggccggggc aggagtggga aacggcgagc cggttgctga cagaacgtaa gttaagcctg
    24721 gagccattaa tcgctcaccg tggaagcttt gaaagcttca cccaggcggt gcgtgacatc
    24781 gctcgtaatg ccatgccggg caaagtgttg ctcattccat gaaaccgcgg gccagcgtga
    24841 tgctggcccg gtattgtgca aaacagatca ttcaccaatg gtcccccttc gtttacacta
    24901 gccacaattg aatatgggta aatgacgatg aattcattcg agcgaaggaa taagatcatc
    24961 caattagtga atgaacaggg aaccgtgctt gttcaggatc tggcgggagt atttgctgcc
    25021 tcggaagcga caatccgtgc cgatttgcgc tttctcgaac aaaaaggcgt ggttacgcgc
    25081 tttcatggcg gtgcggcgaa aataatgtcc ggtaatagtg aaaccgagac ccaggaagtc
    25141 gggtttaaag agcgatttca gctcgccagc gcgccaaaaa acagaatagc gcaggcggca
    25201 gtcaaaatga tccatgaagg gatgaccgtt attctcgaca gcggaagtac cacaatgctt
    25261 atcgccgaag gattaatgac cgctaaaaat atcacggtga ttaccaacag tctcccggca
    25321 gcgtttgccc tttccgaaaa taaagacatt actctggtcg tctgtggtgg cacggtccgc
    25381 cataaaacgc gctcgatgca tggttccatt gccgagcgtt cattgcagga tattaatgcc
    25441 gatttaatgt ttgtcggtgc tgatggtatt gatgcggtta atggtattac gacctttaat
    25501 gaaggttatt cgattagcgg ggcgatggtc accgccgcaa ataaagtgat tgccgttctc
    25561 gattcatcga aatttaatcg tcgcggtttt aatcaggtac tgccaatcga aaaaattgac
    25621 attattgtta ccgatgatgc tgtgtcagag gtggataaat tggcattgca gaagacacgg
    25681 gtaaaattaa ttacggtgta gtattgttct gtatgccgga taaggtgttt acaccgcatc
    25741 cggcattacg gattctccct ggttcaagag agaatagttt gattaacgca ataatggaca
    25801 atctggtggt aatcgacaac gcaacgccgc cggaagtatt tcaatatgga agttttgccc
    25861 actcaacggt tcgccatcaa gattaaaagt gatttcgtgt ggggcttgta tatcaaacca
    25921 cgacgaagcg ccttcgataa tattcgggtt atcttcgtca gattttaagg ttgatacgag
    25981 agccggaaga atttcatcgc cggtaaaaat gcgcagttgc agcaagccat cgttaattaa
    26041 cgcgttcggg cacaattgtt gaccgccacc ggcctgacgt ccgttaccaa taccaatgac
    26101 cagggcgtca ccttgccagt gaaagttttc accgcggatt tcacaacggt ccggttgcag
    26161 agtatccatg cgcattaagc catgaatgat gtaagagacg ccacccagcg cggcttttaa
    26221 tttttccggc gtttctgtgg taatacgcgt cccaaatccg cctgtcgcca tattaataaa
    26281 acaggtttgt ttgttgacct gcgccatatc tatcgcaatg gcattaccgg caattgccag
    26341 tttcagcgcc ttatccagtg cctcaggaat ccctacactg gtggcaaaat cattggcagt
    26401 tcctaatggc aaaattccca gcgcgggtat gtcatccccc tcacactgaa tcaacgccgt
    26461 agaaacttca ttaatggtgc catcgccacc accggcaatc accgttgcga cgcccaactt
    26521 ccgggcctcc tctacaaatc gtgcggcatc acctttctcc caggtgaccc gcacatggat
    26581 cgtcattcct tcctcacgca acagcataat tgcttcgcgc aagggtagat tgtcagtact
    26641 tttaccatta agaatcagta agctggcggg aaattctgcc atgatcgtat gtgcctttat
    26701 gattggtctg tagatagtgt agagcagaaa acaaaaggtg gagtcagaac aggatgaaag
    26761 tcggaggata aaaagtcagt tcaagtatgg aggattcagt ggttaccacc aggagcttgc
    26821 aatggggaca gcgaatacta acggctgcca ccgataaatt tcaaaaaaga gcatatacct
    26881 aatattcaac taaacagtgg catcttcaat ataatatatt aaagccccca tggagttacc
    26941 ctgaagggcc tcaatgtccg taattcctac ttatgtagga aatgttgtac agaacattta
    27001 ttataatcct attcaattat aataatcatg ccattattat atttaaacac tagagagtgt
    27061 cgttggtatt taatggggga aggtgagatg aaaaagatag ctgctatatc attaattagt
    27121 atttttctta tatctggttg tgctgtgcat aatgatgaaa caagtatcgg taaatttggt
    27181 cttgcatata aaagtaatat tcagcgtaaa ctcgataacc aatactacac cgaagccgaa
    27241 gcttctttag ccaggggcag aatatctggt gcagaaaata tagtaaaaaa tgatgcagcc
    27301 catttctgtg ttactcaggg caaaaaaatg cagatagttg acctgaagac agaaggtgca
    27361 ggattacatg gcgtcgctcg tctgacattc aaatgtggag agtgagaata tttttttgta
    27421 agcgtcaaac atgcgcgttc tggctgtgcg tagccggaac ctgggcgagc acgataccgt
    27481 tacgtgaaag gcattgtgct atgaaggggg attctatcga tgtggtcaat ggaagacggt
    27541 tatcagggat caatccttct ttacagctta tgtacaaaaa ataagcccgt gtaagggaga
    27601 ttacacaggc taaggaggtg gttcctggta cagctagcat tttatggatt atgtttttca
    27661 gcgaaacgga tgataacctt aataaatgca gctgtatgtg atcggtttct aagaattttc
    27721 catccgggaa aaataatcga aattaatcac ttaccgtggg ggttacgcgt ggtttccccg
    27781 gagaaattac gcattagcag cgcgtaattt agctcaagat cctgcgggac cgggagccac
    27841 acagtataac catcgcctgg tgcgaccggc atagcttcgc ctttggcgtt ttccatgtgc
    27901 tcaagggtaa agttaatgtt gccttgcggc gtcatcagct caagactgtc gccaacggag
    27961 aatttatttt tcaccgctac cgctgcgagg tcccccttgc gctcaccggt aaactcacca
    28021 acaaactgct ggcggtcaga aactgaataa ccgtattcgt agttctgata atcgtcgtga
    28081 gtatgacgac gcaggaaacc ttcggtatag ccacgatgcg ccagaccttc cagagtttcc
    28141 agcaggctgg tatcgaacgg ttttcccgca gcggcgtcat cgatagcttt gcggtaaacc
    28201 tgtgcggtgc gtgcgcaata gtagaaagat ttggtacgac cttcgatttt cagcgaatgc
    28261 acgcccattt tggtcaggcg ttctacatgg gcgatggcgc gcagatcttt cgagttcatg
    28321 atgtaagtgc cgtgctcatc ttcaaacgcg gtcatatact cgcccggacg ctgggcttct
    28381 tcgatcataa acactttgtc ggttggcgcg ccgataccca gcgtcggctc aacattttgt
    28441 accggaatcg gctcgtactt gtgtacgatg ttgccaacgt catcttcttt cccttcctgg
    28501 acgttgtact cccagcggca ggcgttggtg caggtgccct ggttcgggtc gcgcttgttg
    28561 atatagccag agagcaggca gcgaccggag taggccatgc acagtgcgcc gtgaacgaag
    28621 atctcgatct ccatatccgg cacctgatta tggatctctt caatctcttc cagcgacagc
    28681 tcgcgagaga ggatcacgcg ggtcagtccc atttgctgcc agaatttcac cgtcgcccag
    28741 ttcacggcgt tagcctgcac cgagaggtgg atcggcattt cagggaagtg ctcacgcacc
    28801 agcataatca gccctggatc ggacataatc agcgcatccg gccccatttc caccaccggt
    28861 ttcaggtcac ggataaaggt tttcagcttg gcgttgtgcg gtgcaatgtt gaccacgaca
    28921 taaaactttt tccccagcgc gtgggcttca ttgatgccga gctgaagatt ttcgtggttg
    28981 aattcgttgt tgcgcacacg tagggagtaa cgcggctggc ccgcataaac agcatctgcg
    29041 ccataagcga aagcgtaacg catatttttc agcgttcccg ccggggaaag gagttccggt
    29101 ttaaacataa ttctctcgtt ctgatgacag gtcagatccg cttcacctga tgaagcggtt
    29161 aggggaggcc cccactttaa gggcgggcat tgtagcgctg taggggggat ctggtaaagc
    29221 gccggacgcc aacaacccac gcccggcatc gtaattacgt attgtctttc accgtctggc
    29281 ttgtgccatt ggctttaacg gaggcaattc cggtttcacg agattgcgcg gtggcgtaca
    29341 tctggctgga gccgataatt tgatgattag cggctttcag attgaaatag aatttgccgt
    29401 tgcttgccgt tttttctcat agcgctcttc ttgcgggctg ttgctacgca ccgacgcgat
    29461 gcccttttcc gcagaggctt ttgaggtata cagctcgctg gtgaggatag tctcaccatt
    29521 gcccgctttt agcacaaacc ggaactgatt atcactgctc ttactgagtt caaaccaacc
    29581 agccataatg actccttgat tgaaattgct tatcgaaaac agcatctaaa ggtgctgttt
    29641 actgattaaa gtatgagcga ttttctgatg cctgccaggg cggagagata atgtcagttt
    29701 gctgaccatg cgtggaataa tgttgccgga tcgggtctga acgccatctc cggctaacaa
    29761 agtaatgtcg ctaaaactaa acgatgcggc aggcgtcggc ttcccagcgg taaccaacgc
    29821 cgtaaacggc gcggataaat gactgttcgg cgtcgagaga ttccagcttg cggcgcagat
    29881 ttttaatgtg gctgtcaatg gtgcggtcgg ttactacgcg gtagtcgtca taaagatgat
    29941 tgagcaattg ctcgcgggag aacacttttc ctggttcgtg agagagcgtt ttaagcagac
    30001 gaaattccgc aggcgtcagg tcaagcattt taccgcgcca tgaagcctga aaacgacctt
    30061 cgtcgataat caacgggctt tcggcatcct gttgctgaag ttcgcgttgc ggtttacaac
    30121 ggcgcaaaat ggttttgaca cgcgctacca cttcccgtgg gctgtacggc ttgcagatgt
    30181 agtcatcggc gccaatctcc agccccagca ggcggtcgat ctcttcgatt tttgccgtca
    30241 ccatcacgat cggaacatcg gaaaaacgac gaatttcccg gcataatgtc aggccatcgg
    30301 tgccagggag catcagatcc aacaggatca gatccggtgg tgtctggcgc acatacgcca
    30361 gtacctgatc gccgtggctg ataagcgtcg gcgcatagct cgcagcacgc agataatcaa
    30421 tgagcaactg ccccagcttc ggttcatctt ccacgatcaa aatacgcggt gtgttttcgt
    30481 cgattggtaa ctcggtcata cttctctctg taaatcccgt tccagcggta actctactgt
    30541 aatgcttacc ccgccaaaag gcgaatgggc ggcaataatg cggccattat gggcttcaac
    30601 aatgttcagg caaatcgcca gccccagccc ggaaccgccg ctggcgcggt tgcgggaacc
    30661 ttcggtgcga taaaaacgtt caaacaattt ttgtagctga tcgtcactga cacctggcgc
    30721 actgtcggca aaggtcaggc gcaccgtttt gtcgtgctgc tcggcagaga ttttcaggct
    30781 gccgccgctg tcagtgtagc gcaggctgtt ttccagtaag ttattgaata actgcattaa
    30841 acggtcgcga tcgccaaata cggtgatact gtctggcagg gaaaattgca gtttcaggcc
    30901 acggctggcg aatcgttcgc gaaatgcgcc gcccgccact tccagcagtg ggatcaaatc
    30961 taccggtgct ttttgatagg cgagagcgcc ttcatcagac atcgacaact gatgaagatc
    31021 gtcaaccagt ttggtcagtg taccgacctc cgcctgtaaa gacgccaccg tctccggcgt
    31081 gaatttgcgc acgccatcct gaatggcttc cagttcaccg cgcagcaccg ctaatggcgt
    31141 acgcagttcg tgagaaatat cggccataaa atcgcgccgc atttgctggt ttttctccag
    31201 cgtgctggcg agctggttga agtcttgcgc cagtttgccc agttcatctt cactggtggg
    31261 cgttacgcgg gtagtgaaat cgcccgccgc cagtttgtgc gtgccatcga caagtcgttt
    31321 taccggtgcc agcaaaccgc gcgccagcag aaaggtggcg agcgccgcga gtaacgttgc
    31381 cagggcgacg atcagccagc tggtttgccg ctgttgttta tcgaaattga tatcagtatt
    31441 gcgggtcagc cgctcaacag gggaggcaat caccgcgccc acttccgcac cgttgaccag
    31501 aatgggtcgc cgtgtaccgt caggtggaat cggcgctcgc ggaccaacca gtactttgtt
    31561 gttttgatcg accacccaga actgggtacg ccagccgtgc ggcggcatac ccgggccggg
    31621 tttatcttcc gaattatcgt gttcaaatga acgcaggatc tgaaagacaa agcgatcgtt
    31681 gttgcgcagg aagcgccagt taccatgctg cgcatactgc tcgccaagcg catcacttaa
    31741 cagctgtaat cgctgttcat tgccgtgttt gatgtaatca ataaagccac gctcaaaact
    31801 gatacgcacc gcccagtgca tactgatcag caagacaatg caggtggcga aaatcgccag
    31861 aaacagtttg ccggtaatac cgggtcgcca gaacttcatt gcgcgctcct ttttcgccgc
    31921 gaaatagcta cattttgatg cgtatcgttc ggcactctgg caaagatgaa cgccggaagg
    31981 gcgatgataa acgccatgct aagccaggtg tacataaaga cggtttgtgt ggtgccgctg
    32041 tcgacgctga tatgctgcga accaaaaagc cccagcaaca gcccggcgat ggtgacgccg
    32101 atactcatcg acaattgcat aatcatcgac agcaggctgt ttccgctgct cgccagattg
    32161 tccgggagat ctttcagcgt cagggtgttc atggaggaga aacgggtcga gttgaccatc
    32221 ccttgtaaaa acaggacgaa cggcaaaacg tagtaccagc ccaacagagc ggtagtcata
    32281 aacaacaggg tgaccagcga cagacccagc gtggtcgcta ccagtacccg acgataacca
    32341 aagcgattca ccacctgtac cacaattcgc ttcattccca tgctgccaag caccatcggg
    32401 atcatcatca gtccggcatg aaacggcgag aaaccgaggc caatttgcag gaaaaccggt
    32461 gtcataaagg gcaacatgcc actgccaata cgtccggcaa agctccccgc caggcccagc
    32521 gaaaaggtac gagtgcggaa cagtttcaga ctgaacaggg cgcggttgtt atttctggcg
    32581 tgcagcagat aaagcaccag tgccaccacg ccaactgcga ccaggccagc aatcgccagc
    32641 ggcgataaac ctgtaccttt actgccgtcc agcgccaggg ttaataccgc catgccaacc
    32701 gccagcaata aaaagccgga aagatcaaag cgccgcgtct gcatggtgta gttcggcatt
    32761 aacatcaatg tggcgatcgc accgataatc cccaccggaa tgttgatcaa aaagatccag
    32821 tgccacgacg cgtattctac cagcagaccg ccgagcgccg gaccgagcag cggaccgacc
    32881 tgaccgggta acgtgacaaa ggtcatcgcc gccatatatt gctcgcgcgg tacgattttc
    32941 atcaccgtca acctgccgac cggcaccatc atcgcaccgc caacgccctg taacgcgcgt
    33001 gccagcaaca gttcgttcag cgtgccggaa agcgcgcaaa acagtgaacc gagagtaaac
    33061 agcacgatgg cggtaaagaa aatattgcgc acgccgactt tgtccgccag ccagccgctg
    33121 gcgggcagca tcaccgccac ggtcagcaca taagagacaa tgaccatgtg catatgcaac
    33181 ggactttccc cgaggctttg cgccattgag ggaagggcgg tgtttacgat ggtggtgtcc
    33241 agcgactgca taaagaagcc gaaagccaca atccataatt gccagcgggt gctgtcggga
    33301 agatctgtca tttactcggt taccgtttgt ttaggtttac gcgaaaaacg cagccgcaga
    33361 cggtcgaaaa agagatacac caccggcgtg gtatacagcg tcaggagctg gctcatcacc
    33421 agaccgccga caatggtgat ctccaggggt tgccgcagct ccgagccgtc accgcccgac
    33481 aataccagcg gcagcgcgcc aaacagcgcc gccagggtgg tcatcataat cgggcgaaaa
    33541 cgcagcagac aggcctggaa aatagcttcc tgcggcgtca ggttaccgtg ccgttgggct
    33601 tcaagcgcaa aatcgaccat cataatggcg tttttcttca cgatgccgat taatagcatg
    33661 atccctatca gggcgattag gctgaacggg gcattgaaca gctccagcgc caacagcgct
    33721 ccaacgcccg ccgagggcag ggtggagaga atcgtcagcg gatgtacgta actctcgtaa
    33781 aggataccca gcacgatata caccgtggcg atggcggcga taatcaggat cacctgcgag
    33841 ttcatcgtct cctggaaaac ctgcgccgtg ccggcaaaac tgccgcgcac cgtcgaaggc
    33901 acaccaagct gggtcattgc gcgatcgatc gccgcactgg cgtccgagag cgattttccg
    33961 gtcggcaggt tgaacgagat ggtcgaggcc gccgataatc cctgatgatt caccgatagt
    34021 ggggcattcg ccggttgcca tttagcgaaa tacgacagcg ggatcgcttt gccttcgtta
    34081 ttgataacga acattttttc cagcgcactg atgtcctggg tatagcgcgg atccacttcc
    34141 atcaccactt tatactggtt catcggctgg taaatggtcg agatttgccg ctgaccgaag
    34201 gcgttattta acagactgtt ggcggcctgt acgtcgattc ccagccgtgc catggtgtcg
    34261 cggtcgtaaa ccagattcat ctccgcgccg ttatcctgct gatcggagtt cacgtccgcc
    34321 agttccggca acgtcgccag ttttttgcgg attttcggct cccattcgcg cagtgccgcc
    34381 aggtcgtcgg ataacaacgt gtactggtag ctggcgttcg actgacgccc accaacgcga
    34441 atatcttgta ccgccatcag gaacagattc gcccccggtt ctttcgccag cttcacgcgc
    34501 agacggtcga taatttgctg cgccgtttcg ctgcgttcgt cgcgtggctt gagggtgata
    34561 aacatcatcc cactgttaac tcgtgaaccg ccggtaaagc cggtgacatt atccactgcc
    34621 ggatcgtcac ggataatttt catgaaatcc tgcaatttgc cgcgcatcgc ctgaaacgaa
    34681 atactctgat ccgcctgtat cccacccatc agtacgccgg tgtcctgctc cgggaagaag
    34741 gttttcggga tcgagatatt cagcgcaatg gtgccaagca gcaccacgcc caccagacgg
    34801 gtatgattga gcacccattt cagcgacttg ccgtagcctt gttgcagggc taccaacatg
    34861 cggccaaaac cacgcagtcg cttttgctca cgcggcttgc tggcttttag catccagccg
    34921 cacatcattg gcgttagcgt cagggaaacc agcaacgaaa taccaatggc gacagaaagc
    34981 gtcacggcaa actcgcgtaa cagtcggccc ggcaatccgc ccatcaacag cagcggcagg
    35041 aacaccgcca ccagtgacag actcatcgac agcaccgtaa agccgacttc gcgagtacct
    35101 tgcagcgcgg cctgtagtgg tttcatcccc gcttccagat gacgggcaat gttttccagc
    35161 accacaatgg cgtcatccac cacgaaacca gtagcaatgg tgagcgccat cagcgaaaga
    35221 ttattgagac tgaatccgca caggtacatc gccgcaaacg taccaatcag cgaaaccggc
    35281 accgcaacgg cgggaataat agtggcgcga cccgagcgca ggaataaaaa gaccaccaga
    35341 atcaccagcg ccaccgagat aatcagcgtt tgctcgactt cttccagcga ggcgcgaatg
    35401 gtgggggagc gatcctgggc aatttgcaga tcaatcgccg ccggaatggt ctcctgcaac
    35461 tctggtaatt ttgcccggat gctgtctacc gtctggataa tattggcttc cggcagtttg
    35521 cggatcatca gtaaaatagc cggtttggcg ttggtcatcc cggcgttgcg cacatcctgc
    35581 actgagtcag tcaccgtcgc cacatcgccc agacgaaccg cgccgccgtt gttgtagtga
    35641 ataatcagcg gctgatattc agcggcggtt ttcagttcat cgttggtctg gatctgccag
    35701 cggtgagtgc catcttccag cgccccctgc ggtttacgca cgttggcatt gctgatggca
    35761 gtgcgtacgt cgtccagcga cacgccctga ttaaacagcg cctgcggggt cagcccgacg
    35821 cgcacggcgg gcagcgagct gccaccgaca tcgacatcac cgacaccgtc gatttgcgaa
    35881 atcgtcggag ccagctgcgt cgaggcgaaa tcgtacagtt caccctgcga ataagtatcg
    35941 gatgtcagcg tgaggatcat aattggcgca tccgacgggt tcgctttgcg ataggtcggg
    36001 cggctgggca tcccgctggg cagcaaactt tgtgcagcgt tgatcgccgc ctgcacatca
    36061 cgcgctgcgc cgttgatatc ccggtcaaaa tcaaactgca aaataatgcg cgtgctgccg
    36121 agcgaactgc tggaggtcat ttcactaacc ccggcaatgc gcccaagtga gcgctccagc
    36181 ggcgtggcaa cggaagacgc cattgtctcc ggcgacgcac cgggcagcga ggcgctgacc
    36241 ataatcaccg gaaaatcgac ctgcggcagc ggggcgaccg gcagcatacg gaaacccagt
    36301 atgccgcaca gggtaatggc gaccgacagt aaaatcgtcg ccaccgggcg gtaaatgaag
    36361 agggcaaaaa acttcactta cgcctcctct tcatggcgag caaagcgact tttggtccac
    36421 aatgccaggc ggtcgaacag cagataaatc accggcgtgg tgaacagcgt cagcacctgg
    36481 ctgacaatca gaccgccgac catgccgata cctaacggac gacgcagttc cgcgccgacc
    36541 ccggtactca acatcagcgg cagcgcgcca agcagagccg ccagggtggt catcaggatc
    36601 ggacgaaaac gcaacagaca agcctggtag attgcatcgc gcggcgacat gccttgctcg
    36661 cgctcagcag ccagcgcgaa gtcgatcatc atgatggcgt tcttcttcac aataccgatc
    36721 agcaaaataa tgccgataat cgcaatcaca tccagttcgc taccggcaat cattaatgcc
    36781 agcagcgcgc cgacccctgc ggtgggtagc gtcgagagaa tggtgatcgg gtgaataaag
    36841 ctctcgtaca gaatgccgag cacgatatac atcgccacca ccgccgcgac aatcagccag
    36901 acagtgctgc ccagcgccga ctggaaggcg agggtgctgc cctagaattg cgtggtgata
    36961 tccaccggca gattcagcgt cttttcggtg tccataatcg cctgcaccgc atcgcccagc
    37021 gaatagttat ccggcacgtt aaaggagatg gttgttaccg ggaactgatc cagatggttg
    37081 atggaaagcg gcgcaaaacg ctgctcaatt ttggcaattg agcttaacgg caccacgcca
    37141 ccgtcgctgc tggtcaggcg aatggtatcc agcgccgcga ggcctggggt attttcggta
    37201 ttgtgctcca gcaccacgcg atactggtta gcctgagtat aaatagtgga aatcagtcgc
    37261 tgaccaaacg cgttgtacag ggcgttatcg acgtccgcca tgctgatccc cagacggctg
    37321 gcgctgtcgc gatcaacatt gacatacgcc accagccctt tgtcctgcca gtcgctggag
    37381 acatcagaaa gctgtggcag ttgctggagt ttttccatca actgtggcac ccaggtactg
    37441 agcgcatcca gtgacgtggc ctgcaaggta aactggtact gggtgcggct gacctgagta
    37501 tcgatggtca ggtcctgcgt tggttgcagg aagagatcga cgcccggcac tttatctacc
    37561 gccgtttgca gacgggcgat gactttttgc acccgatcat cacgttcatc caacggtttg
    37621 aggttgattt gtaaacgtgc actattcagc gacgggttag tgccatcaac gccaacaaat
    37681 gaggtcaggc tttgcactgc cggatcctgc aaaatcacgt ccgcgacctg gcgttgtcgc
    37741 tgggccatat tggcaaagga gctggattgc ggtgcctgca aagtgccctg aataatgccg
    37801 ttgtcctgta ccgggaagaa acctttcgga atgaacaccc acagcagcac gctaagcagc
    37861 agcgtgctga gtgccacgct taaggtcagc cacggatgat tcagcacttt cgccagtcca
    37921 cgaccatagg cggcgattat cctgtcgaac attttttccg aggcacggga gaagcggttc
    37981 tgtttacgca acgactcctg gctgagcatc cgcgcgcaca tcatcggtgt cagggtcagc
    38041 gacaccaccg ctgagatcaa aatcgctacc gccagggtaa tagcaaattc gcggaacagt
    38101 cgcccgacga tatcgcccat aaacagcagt gggatcaaca ccgcaatcag tgagaaggtc
    38161 agcgagataa tggtaaagcc gatttcacct gcgcccttga gcgccgccgc caacggtttt
    38221 tcgccttttt cgatatagcg ggaaatgttt tcgatcacca cgatggcgtc atcgaccacg
    38281 aatccggtgg cgatagttaa cgccatcagt gtcaggttat tgattgaaaa atcgagaaac
    38341 accataaccg cgaaagtgcc gattaacgac agcggtacag caacaccggg aatgatggtc
    38401 gccggaatat tgcgcaaaaa caggtagata atcatgacta ccagcgcgat agccatcatc
    38461 aattcaaact gagtatcatc gacggatgcg cggatattgg tggtgcgatc ggaaagcact
    38521 gtcaccttca ccgatttcgg cagactctca gtgagctgtg gcagcatctg ccgaatgctg
    38581 tcggcggtgg agataatgtt agcaccgggc tggcgctgaa cattcatcac aatggcctgt
    38641 tctttgttcg cccacgcgcc gagccagctg ttttctgcac cttgctctac agttgcgaca
    38701 tcgcccagac gaattggcgc gccgttctgg taggcgatga ttagctggcg atactcttcg
    38761 gcggattgca tctggtcgtt cgcggaaagc gtgaccgcac gggaagggcc gtcgaggcta
    38821 ccttttgccg agttaacgtt agcgccggta atggcggtgc gcacggtttc gctggtcagg
    38881 ccgagggcgg caatcgcctg agcgttaagt ttgacgcgaa cagccggacg ctgaccgccg
    38941 gaaagcgtca ccaggccgac gccggaaatc tgcgagattt tctgcgcgac gcgggtttcc
    39001 accatatctt ccacttgcgt catcggcatg gcggttgagg tgacggcgag cgtcatgatc
    39061 ggcggatctg ccgggttcac tttgctgtaa accggcgggt taggcagatc gctcggcaac
    39121 aagttggtcg cagcgttaat cgcggcctgc acttcctgct cggcgacatt gagcggtaat
    39181 gttagctgga actgcaaagt gataactgac gcaccgccgg aactttgcga cgacatctgt
    39241 ttcaggccag acatctgccc gaactggcgt tcgagcggcg cggtaacggc agaggtcatg
    39301 acatccgggc tggcacctgg gtagagcgtg accacctgaa tggtcggata gtccacttcc
    39361 ggcagcgccg aaacgggcag ggcgcgataa ccgataatcc cggcgagtaa gatcgccacc
    39421 atcagcagcg tggtggcgac aggacgcata ataaacaggc gcgacgggcc gcctgtgctg
    39481 ctcgggggta acacctgcat caggagcgtg ctcctttttt cgcgtattcg cggctggtgg
    39541 ctttctcttc cggagtggtg gcgctctggg cttccaccac ttccactttc gccccttcgg
    39601 tcaggcgatc aatgccgtct gtcaccacgc gatcgcccgc agaaatacct gcacggatca
    39661 ccactttctg actgtcctga atgcccggcg tcaccagatg tttgctgacc ttgttttcgc
    39721 tattcagcac ccagacaaaa tggccttcat tgcccatttg cagggcggct gttgggatca
    39781 ctacggcgtt ttgttcggtg tcgactaaca tgcgcgcgtt aacaaactga ttgggaaaca
    39841 gcgcatcatc ctgattatta aagcgtgctt tcactttaat cgtaccggtt gtggcatcga
    39901 tttggttatc gaggcttaac agcgtgcctt cacttaattt cttcgaattg gtgcgatccc
    39961 aggcttctac catcagcggt tttccggctt tttgcgcctg cactacagta gcgatatcgc
    40021 tttccggcag ggtaaagact aaatcgatag gatgcgtctg ggtgatcacc acaattccgg
    40081 tggtatcacc actggagatt tggttaccaa catcaacttg cttgagacca acgcgaccat
    40141 cgactggtgc ggtgatgcgg ctccagtcga gttgcaactg cgcgctggcg acgcttgctt
    40201 catcagcctt aatggtgcct tcggtttcac tgaccagcgc ctgttgggca tccagctcct
    40261 gacgggagac gagattggtt tttgccagtt gctgataacg cgccagatca cggcgggcgt
    40321 tagcaagcgt ggctttatct tttgccagtt ggccctgggt ttgtgctaat gcaactttga
    40381 actggctggg gtcaatttct gccagtaaat cgcctgcttt gacctgctgg ccttcctgga
    40441 aatgtaacgc catcagttga ccgtccacgc ggctgcgcac cgtaacagta ttagcggcgg
    40501 taatggtgcc aagcccggtg aggtaacgtg cccctattaa gttgtcaagc atgttatgac
    40561 ccctgcgggg tataaaaagt cccgtcgcgc atcatggcga acagaa
//
LOCUS       DAOFMX010000031        40597 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.031, whole genome shotgun sequence.
ACCESSION   DAOFMX010000031 DAOFMX010000000
VERSION     DAOFMX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.031"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(1..956)
                     /locus_tag="QBA20_001832"
                     /pseudo
     CDS             complement(1..956)
                     /locus_tag="QBA20_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="QBA20_001833"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="QBA20_001833"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDQ6292390.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="QBA20_001834"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="QBA20_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="QBA20_001835"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="QBA20_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDQ6292391.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="QBA20_001836"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="QBA20_001836"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDQ6292392.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="QBA20_001837"
     CDS             complement(5018..6346)
                     /locus_tag="QBA20_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDQ6292393.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8702)
                     /locus_tag="QBA20_001838"
     CDS             complement(6354..8702)
                     /locus_tag="QBA20_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDQ6292394.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="QBA20_001839"
     CDS             complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="QBA20_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDQ6292395.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="QBA20_001840"
     CDS             complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="QBA20_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDQ6292396.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="QBA20_001841"
     CDS             complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="QBA20_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDQ6292397.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="QBA20_001842"
     CDS             complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="QBA20_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDQ6292398.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="QBA20_001843"
     CDS             complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="QBA20_001843"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDQ6292399.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15317..16552
                     /gene="moeA"
                     /locus_tag="QBA20_001844"
     CDS             15317..16552
                     /gene="moeA"
                     /locus_tag="QBA20_001844"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDQ6292400.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16552..17301
                     /gene="moeB"
                     /locus_tag="QBA20_001845"
     CDS             16552..17301
                     /gene="moeB"
                     /locus_tag="QBA20_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDQ6292401.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="QBA20_001846"
     CDS             complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="QBA20_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDQ6292402.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18273..19172
                     /locus_tag="QBA20_001847"
     CDS             18273..19172
                     /locus_tag="QBA20_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDQ6292403.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19178..21610
                     /locus_tag="QBA20_001848"
     CDS             19178..21610
                     /locus_tag="QBA20_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDQ6292404.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21756..22571
                     /gene="ybiV"
                     /locus_tag="QBA20_001849"
     CDS             21756..22571
                     /gene="ybiV"
                     /locus_tag="QBA20_001849"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDQ6292405.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22723..23988
                     /locus_tag="QBA20_001850"
     CDS             22723..23988
                     /locus_tag="QBA20_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HDQ6292406.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24229..25821)
                     /locus_tag="QBA20_001851"
     CDS             complement(24229..25821)
                     /locus_tag="QBA20_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDQ6292407.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26040..26960
                     /gene="ldtB"
                     /locus_tag="QBA20_001852"
     CDS             26040..26960
                     /gene="ldtB"
                     /locus_tag="QBA20_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDQ6292408.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27018..28136)
                     /locus_tag="QBA20_001853"
     CDS             complement(27018..28136)
                     /locus_tag="QBA20_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDQ6292409.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="QBA20_001854"
     CDS             complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="QBA20_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDQ6292410.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28786..28914
                     /gene="mntS"
                     /locus_tag="QBA20_001855"
     CDS             28786..28914
                     /gene="mntS"
                     /locus_tag="QBA20_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDQ6292411.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29186..30769
                     /gene="opgE"
                     /locus_tag="QBA20_001856"
     CDS             29186..30769
                     /gene="opgE"
                     /locus_tag="QBA20_001856"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDQ6292412.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="QBA20_001857"
     CDS             complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="QBA20_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDQ6292413.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31385..31450)
                     /locus_tag="QBA20_001858"
     CDS             complement(31385..31450)
                     /locus_tag="QBA20_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292414.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31685..32572
                     /gene="rhtA"
                     /locus_tag="QBA20_001859"
     CDS             31685..32572
                     /gene="rhtA"
                     /locus_tag="QBA20_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDQ6292415.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32871..33374
                     /gene="dps"
                     /locus_tag="QBA20_001860"
                     /gene_synonym="pexB"
     CDS             32871..33374
                     /gene="dps"
                     /locus_tag="QBA20_001860"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDQ6292416.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33778..34524
                     /gene="glnH"
                     /locus_tag="QBA20_001861"
     CDS             33778..34524
                     /gene="glnH"
                     /locus_tag="QBA20_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDQ6292417.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34663..35322
                     /gene="glnP"
                     /locus_tag="QBA20_001862"
     CDS             34663..35322
                     /gene="glnP"
                     /locus_tag="QBA20_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDQ6292418.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35319..36041
                     /gene="glnQ"
                     /locus_tag="QBA20_001863"
     CDS             35319..36041
                     /gene="glnQ"
                     /locus_tag="QBA20_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDQ6292419.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36302..38526
                     /gene="ybiO"
                     /locus_tag="QBA20_001864"
                     /pseudo
     CDS             36302..38526
                     /gene="ybiO"
                     /locus_tag="QBA20_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38523..39449)
                     /gene="rlmF"
                     /locus_tag="QBA20_001865"
     CDS             complement(38523..39449)
                     /gene="rlmF"
                     /locus_tag="QBA20_001865"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDQ6292420.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39725..39985
                     /locus_tag="QBA20_001866"
     CDS             39725..39985
                     /locus_tag="QBA20_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ6292421.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40250..40579
                     /locus_tag="QBA20_001867"
     CDS             40250..40579
                     /locus_tag="QBA20_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDQ6292422.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     NASQLSN"
BASE COUNT        10115 a        10734 c         9871 g         9877 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctaggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattactgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accagattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctag tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttcacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgaatctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc
     8701 atgttaatac atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct
     8761 tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact
     8821 tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac
     8881 tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc
     8941 agtacggtca gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata
     9001 tccgctcgtg cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg
     9061 ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac
     9121 accacgatag aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg
     9181 gcaccaatac tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg
     9241 cgcaccaggc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca
     9301 atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac
     9361 agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac
     9421 aagcccagca acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc
     9481 gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca
     9541 aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg
     9601 tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat
     9661 gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta
     9721 cgtacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta
     9781 aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca
     9841 ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg
     9901 ggtaatcgcg catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa
     9961 ctttctccac cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca
    10021 ctaccgggat catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc
    10081 ctttcgccct cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg
    10141 taaagcgcgc catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt
    10201 gctgccaact gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa
    10261 ttaaaaacat ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac
    10321 tcaatcgatc cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa
    10381 atatcaccgc ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg
    10441 caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag
    10501 cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac
    10561 gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc
    10621 cgggcagcat gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca
    10681 accccagtaa tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa
    10741 atccgcgtct tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt
    10801 actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat
    10861 gatatcctgc gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc
    10921 ctgagccagg aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg
    10981 tggccagttt tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc
    11041 cgaccagcca gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc
    11101 tgccgcccgc tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa
    11161 ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag
    11221 cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg
    11281 atcgtaaggc cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt
    11341 agctggcgtt gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta
    11401 attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat
    11461 ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt tctccagcag
    11521 tgtggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc
    11581 cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc
    11641 tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc
    11701 cagttcatac ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag
    11761 cgctgtcggt gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa
    11821 cggctgtttg agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt
    11881 cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc
    11941 ttttaccgcc actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt
    12001 cacggtgtaa gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag
    12061 tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga
    12121 taacgtgtca ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac
    12181 cacatctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac
    12241 taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca
    12301 tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg
    12361 agacggctgc cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca
    12421 gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac
    12481 gcgtataagg atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt
    12541 gcccgagata catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg
    12601 agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct
    12661 gcccgcgaat agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat
    12721 tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg
    12781 ggtaacgcca ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg
    12841 cagccgcgtc tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac
    12901 cgatggtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc
    12961 ggcgcaatgc ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa
    13021 tttcgccgcc ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc
    13081 tgccagactc gcccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct
    13141 caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg
    13201 tgacaagatt acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg
    13261 ggggttcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta
    13321 accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt
    13381 gcggtgcatg aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca
    13441 ccagtacccg atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc
    13501 ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga
    13561 catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca
    13621 tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa
    13681 tggtttgtgc ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt
    13741 cttcacgact ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa
    13801 atactgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca
    13861 catggcgcat ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc
    13921 gcaacagcat tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg
    13981 ccagcgcagt cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc
    14041 gttgcagact aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg
    14101 caatattaag attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca
    14161 acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag
    14221 gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg
    14281 attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct
    14341 tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg
    14401 cggatgaaga cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag
    14461 catccggcac ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg
    14521 cctgtggacg tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg
    14581 cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc
    14641 tcgcgcgctg ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg
    14701 cgctccatgc catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc
    14761 gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc
    14821 gccaccgcac cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt
    14881 tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga
    14941 cgcaccgctt ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg
    15001 gtttcaacaa tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc
    15061 gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc
    15121 gtccattaag ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca
    15181 ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc
    15241 ccgaggtggc atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct
    15301 ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa
    15361 tgagatgctt tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg
    15421 ttttggtcgt attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa
    15481 ctccgctatg gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc
    15541 cgttgccggt aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg
    15601 cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga
    15661 gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa
    15721 tattcgccgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg
    15781 cctgactacc gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat
    15841 tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc
    15901 gctgggcgac ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca
    15961 gctgggttgc gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc
    16021 cgcatttatt gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt
    16081 gggtgaagcg gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa
    16141 gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg
    16201 cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt
    16261 gctggcaaaa ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac
    16321 agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg
    16381 caacgccgac ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag
    16441 ctcctttagt ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt
    16501 gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa
    16561 ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt
    16621 gacggtcagg aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc
    16681 tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc
    16741 gacacggttt cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg
    16801 caaccgaagg tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt
    16861 acgccagtca atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat
    16921 ctggtgctcg actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt
    16981 gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc
    17041 ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac
    17101 gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg
    17161 caggcaatgg aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc
    17221 gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg
    17281 tgtgaggtgt gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc
    17341 atcaggcatt tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg
    17401 ggcacagatt tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat
    17461 gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct
    17521 caaacttcgt cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca
    17581 gtggcagagt aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt
    17641 tgaaactcgc tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt
    17701 cggtcacagt ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa
    17761 catattccgc acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc
    17821 ccagcgtcgg aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca
    17881 ccggaacttt caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca
    17941 tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt
    18001 catgaagttg cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt
    18061 tagtggtcac acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg
    18121 aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac
    18181 tatcacggga aaactctctg aacagttgac gcacatcaat ctaactttca ttcgaaagta
    18241 ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga
    18301 cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc
    18361 gctggtgtca gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac
    18421 tgtgtctgga aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc
    18481 tgaatggttt gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg
    18541 actgctgtcc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca
    18601 tggcgaccgt tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt
    18661 cgggtggtga acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg
    18721 aggcaggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc
    18781 cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt
    18841 ttaaacagtg gaccgacggt aacgccgcca gggtactgga taacctgaaa aaactcgccg
    18901 cagcgggcaa aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa
    18961 cctctgtaaa agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt
    19021 ttctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg
    19081 ccccggaaaa accgcttgat gcgccagagc tgctcgattt tgcccagcag tatgcctgcc
    19141 agaaagggtt aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac
    19201 gctcagcgac cgcattaaag cgcacaaaaa tgcgctggtg catattgtga agccgccagt
    19261 ctgtaccgag cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat
    19321 cccggtacgt cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa
    19381 acacgatgag ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc cgatcttccc
    19441 ggaatatact gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc
    19501 tggctttgcg gtgagcgaag agaacaaacg tgttctgcat gaagtgtgcc cgtggtggcg
    19561 cggtcagacg gtacaggatc gctgctatgg catgtttacc gatgagcaaa aaggtctgct
    19621 ggcgaccggc atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt
    19681 caatttcccg ctgctgctgg aaaaagggct tgatggtctg cgtgagaaag tagcggaacg
    19741 tcgctcgcgc atcaatctga cggtgctgga agatttgcac ggcgaacagt tcctgaaagc
    19801 gattgatatc gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg
    19861 tgcaatggcc gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctgg cgatggcaga
    19921 aaactgcgat cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg
    19981 ttacttcatc cagttgattt tgcagattga atctaacggt cactcggtgt cgtttggtcg
    20041 tatggatcag tatctctacc cgtactatcg ccgcgacgtt gaactgaacc agacgctgga
    20101 tcgcgaacac gccatcgaat tgctgcatag ctgctggttg aaattgctgg aagtgaacaa
    20161 aatccgctcc ggctcccact caaaagcctc tgcgggaagt ccgctgtatc agaacgtcac
    20221 cattggcgga caaaatctgg ttgatggtca accaatggac gcggtgaatc cactctctta
    20281 cgcgatcctc gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta
    20341 ccatgcagga atgagtaacg atttcctcga cgcctgcgta catgtgatcc gctgcggctt
    20401 cgggatgccg gcattcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat
    20461 tgaaccgcag gacgcttatg actacgcagc gattggttgt atagaaaccg ccgtcggtgg
    20521 caaatggggc tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc
    20581 ggcgctggaa ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc
    20641 gttgtcggca ggtaacttca acaacttcga tgaagtgatg gacgcgtggg atacgcaaat
    20701 ccgttactac acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga
    20761 gaacgtgcac gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag
    20821 tatcaagcaa ggcggcgcga aatatgactg ggtttctggc ctgcaggtcg gtattgccaa
    20881 cctcggcaac agcctggcgg cagtgaagaa actggtgttt gaacagggcg cgattggtca
    20941 gcaacagctg gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg
    21001 tcagcggctg attaacggtg cgccgaagta cggcaacgac gatgataccg tcgatacgct
    21061 gctggctcgc gcttatcaga cctatatcga cgaactgaaa cagtaccata acccacgcta
    21121 cggtcgtggt ccggttggcg gcaactatta cgcgggtacg tcgtctatct ccgctaacgt
    21181 accgtttggc gcgcaaacta tggcaacgcc ggacgggcgt aaagcccaca ccccgctggc
    21241 agaaggcgct agtccggcct ctggtactga ccatctcggc cctactgcgg tcattggctc
    21301 agtgggtaaa ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa
    21361 tccggcaacg ctggagaacg aatctgacaa gcagaaactg atgatcctgc tgcgtacctt
    21421 ctttgaagtg cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct
    21481 ggaggcgaaa aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggttattc
    21541 cgccttcttc accgcgctct ctccagacgc tcaggacgat atcatcgccc gtactgaaca
    21601 tatgctgtaa tctcttcggc ccggctgcgg cgtcgccggg ctaaatcatt cacatcatca
    21661 atttcatcct tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac
    21721 tgttacggtt tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag
    21781 atatggacgg tacttttctt aacgacgcca aaacgtacaa ccaaccacgt tttatggcgc
    21841 aatatcagga actgaaaaag cgcggcatta agttcgttgt cgccagcggt aatcagtatt
    21901 accagcttat ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg
    21961 gcgcactggt ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat
    22021 cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc
    22081 tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac
    22141 actaccatcg cctgaaacct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt
    22201 tttcgctcaa cctgccggat gaacaaatcc cgttagtgat cgacaaacta cacatagcgc
    22261 tggatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg
    22321 gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa
    22381 atgtggtagc gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt
    22441 cctttgcgat gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg
    22501 ataataatca tgaaggcgcg ctgaatgtga ttcaggctgt gctggataac acatcccctt
    22561 ttaacagctg acctctctac cggagccatg cgctccggtt ttcccttctt tttacatcct
    22621 gtacatcaca tcaaatcatt aaacttgcat taacttattt ataacttgta ttgtcacatg
    22681 caaattagca tagtttcgaa tgaaagttaa tgcgaggtgg ttatggcttc tacttttacc
    22741 agcgacacat tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg
    22801 gcgcagcttg gcgatgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga
    22861 gtggctgaaa tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat
    22921 gccgatatca aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt
    22981 tgtgcggtga taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg
    23041 ctggactatc tgaaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc
    23101 ttcgggacgc tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa
    23161 atgcaggccc gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg
    23221 acatttgaaa gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac
    23281 cgtatccgcc gccttccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc
    23341 ggggcgctgg aacgctggct gcttccagcg tatcagcacg ttttcgccaa cgtctttaat
    23401 ggcaatctgg cgaaatacga tccctggcat gcggcacatc gtacggaagt tgaagagtac
    23461 acggtggaca acaccaccaa atgttccgtg tttcggacat tccagggctg gactgcgctc
    23521 tcggatatgc tgcctggtca ggggttgctg cacgtcgtgc ccattcctga agccatggcg
    23581 tacgtactgt tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg
    23641 cccggaagag tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc
    23701 agcattccaa aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc
    23761 gttgcccccg ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg
    23821 atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca
    23881 tcgccgggcg acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt
    23941 gccgacctca acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa
    24001 gtgaattgat gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg
    24061 ctttcttaat gcctgatgcg gcgttaacgc cttatccggc ctacaaagtc ctgcagattc
    24121 aatatattac aggtactgcg taggcctgat aaacgtagcg catcaggcta ttttgcattt
    24181 gtcatcagcc gtcgtcaccc cgacagtatc ggggtgacgg ataatcactt actcgatccc
    24241 tttactacgc aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt ccggggtgat
    24301 ttccagaatg cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa caaagatcag
    24361 cgtgccctga tacagttcca gcgccatgtt cagtgactcg atggattcca tatccaggtg
    24421 gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact taccaaacag
    24481 catccgccct ttttccccac cggaaagcac tttagctggc tttttgatgt cgtcctggct
    24541 gaacagcaaa cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt cctgcttcca
    24601 ctgactcatc cattcgaaca cggtcagatc attttcaaac tcatattcgt ggtcctgggc
    24661 gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg gttgcagatc
    24721 gcccaccagc gttttcagca acgttgattt accgacgccg ttggtaccca gtaccgcaag
    24781 tttttcaccg acctccagca gcaggttgag atttttaaac agtggaccgt tatcaaaccc
    24841 tttggtcaga ccttccactt ccagcgcgtt acggaacagt ttcttatcct gttcaaaacg
    24901 gataaacggg ttctgacggc tggaggcttt cacctcttcc agtttgattt tatcaatctg
    24961 gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc ggctaacgaa
    25021 agattgcaac tcagcaatct gcgctttctt cttggcgtta tcggccagca gacgttcacg
    25081 cgcctgggtc gccgccgtca tgtactcatc gtagttaccc ggataaacgc gcagctcgcc
    25141 gtaatccaga tccgccatgt gggtacagac catgttaagg aagtgacggt cgtgcgagat
    25201 gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccagcgaa tggtgtcgat
    25261 gtcgaggttg ttggtcggtt cgtcgagcag gagaatatcc ggctcagcaa acagcgcctg
    25321 cgccagaagc acacgcagct tccagccagg agcaacttca ctcatcgggc cgtagtgctg
    25381 ttccactgga attcccacgc caagcaacag ttcaccggcg cgagcttccg cagagtaacc
    25441 gtccatttcg ccgtatttca cttccagatc ggccacttta tagccgtctt cttcactcat
    25501 ttccggcaaa gcatagatgc ggtcgcgctc ctgcttcact tcccacaact ctttatgccc
    25561 catgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct gacgcagctt
    25621 accaatgcgc tcgttgggat cgagggaaac gttacccagc gtcggctcaa ggtcgccgcc
    25681 gagaatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc cgtaacggtt
    25741 gccgccgccg aatttgacgg aaatgttttc aaacaacggc ttactgccga actgcatggt
    25801 gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa ccgcgcatta
    25861 tgccataagt ctgaaagaag atcctgctcc tcgcttaagg tctacactca agccaaaggc
    25921 aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac ttataatgcg
    25981 cttccaatac tctaatattc tcaacccaat ggcctgccag gcacaaaatc tcgcttaaca
    26041 tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc ttttgcagta
    26101 ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt ggtcagaatc
    26161 aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc gcggagtacc
    26221 agatggggct ttccaatatg atggaagcga acccgggtgt ggataccttc ctgccgaaag
    26281 gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt catgaaggca
    26341 tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaagggacc aacaccgtta
    26401 tcgtgctgcc gatcggcatt ggtcagttag gcaaagatac gcctatcaac tggaccacca
    26461 aagttgagca taagaaagca ggcccgacct ggacgccgac cgccaaaatg cacgcagagt
    26521 accgcgctgc gggcgaaccg ctaccggctg tcgttccggc aggtccggat aacccgatgg
    26581 gtctgtatgc actctacatc ggtcgcctgt atgctatcca tggcaccaac gccaacttcg
    26641 gtatcggcct gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac atcaaattcc
    26701 tgttcgagaa agtaccggtc ggtacccgcg tacagtttat tgatgagccg gtaaaagcga
    26761 ccaccgagcc agacggcagc cgttatattg aagtccataa cccgctgtct accaccgaag
    26821 cccagtttga aggtcaggaa atcgtgccaa ttaccctgac gaagagcgtg cagacagtga
    26881 ccggtcagcc agatgttgac caggttgttc ttgatgaagc gatcaaaaat cgttccggga
    26941 tgccggttcg cctgaattaa tcgtcaaaac gtaaaacaaa ggcggactca taatccgcct
    27001 tttttatttg ccagacctta ccaggccggg atcataacta acaaaacgta tcccaccagc
    27061 gccgcccaca acagcatcgg tattgaatag agatggaaac gccaccagat gcggcgatcg
    27121 ttcgccatac gtagcgcaat caaatttgcc agcgatcctg gtaataaacc aaagcccccc
    27181 acgtttaccg cccatgccag taataaagac ggaggtacat aattgagcag caaaatggta
    27241 ctcggcacgt tactgatcac ctgcgataaa ccgactgccg ttaaccataa cccgggttca
    27301 gataaatgac tcacgttacc caacacgcct tgcaacgctg gaagctgggt cagtaaatgg
    27361 acgtcgataa acatcgccat aaacaccagc agcaacgtcc agtccacact caacaccacg
    27421 cgacgcgcca gcagcacaaa gcccgccgcc acaatcacca gtccccacag ctcttgtttg
    27481 aactccagcg ccgtcagaaa gacgatatac agccccagac aattccacac cagccgcggt
    27541 ttccactccg gtgtttgcac ccccgtatgg tattgcagcg cctttccagg gaaacaacac
    27601 cagcacaaca gcaggagtgt cagcatcatt gcgccagcca tcggtgccat ttgggcaata
    27661 aatccggcaa acgaaagacc agaacgtccc cagataagaa tattttgcgg gttgccaatt
    27721 ggcgtcagta gcgaaccagc gttgactgcc agcgcctcaa aaataatcag ccgattaacc
    27781 gggatctcac acagtctttt tagcgtgata gtcagcggaa caacaataaa cagcgcgaca
    27841 tcgttggtca gaaaggtaga aagcagcgcc gccgccagca ccataaacat cgccagccga
    27901 cgctccgtag caaagcggcg caccattttg cgccccagca catcaaaata accgcttaac
    27961 tccacacctt tggtcagcag catcaggccg cttaaggtga tgatggtgtg ccagtcgata
    28021 gcagcaggcc aggatttcgg tgcaaagggc acgaaaaagc ttaatccgat accaacaaga
    28081 attaataact gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag gctcatttgg
    28141 caccgtgttt ctgggtaaac aaacgaaaag cgtcgagcgt ctcttcacta acatggtgct
    28201 ccatgccttc cgcgtcgcga cgggcgattt ccggactgac gcccaacacc aacaagaaat
    28261 tttcgactat ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct tctgccgtta
    28321 aaaacacgcc acgccagggg atcatttcaa tcagccccat ggttgccagc cgcttaagca
    28381 ttttagccac cgtcggttgc gaaactccca gacgagcagc catgtccacc tgacgagctt
    28441 cccccacttc cctgatcaag tcagaaatca gctcaacgta atcatcaata agctcgcgcc
    28501 gatgcgcctc tctgacctgg cggaaccctt caacgtgctc ttccacgttc actaactgcg
    28561 tcactttttt tgctattggc gtacctgcgc gacgactcat tgtgcttcct cattatggtg
    28621 acgcatacat agcgttctca tttcgtgcgc acattgtaaa ccagagttgc gaaggtacaa
    28681 aaaattaacg ttttagcaat agctatataa tatagcctgt gctatatctg tatgtaatgc
    28741 aatcatccct caaggatcga cgggattagc aagtcaggag gtcttatgaa tgagttcaag
    28801 aggtgtatgc gcgtgtttag tcattctccc tttaaagtac ggttaatgct gctctctatg
    28861 ttgtgcgata tggtcaacaa caaaccgcag caagataaac cttcagataa atagcggcgt
    28921 cgcggtacgc cgcttcactc ctgcttttat gcaggcataa cgcgttttgg tctgaaaaaa
    28981 accacttttt gtcggatttg caatcccctt cgcaaaagat ttgttcgtca gtagttgacc
    29041 tgaacggcgg ctcgctctat cttcttgcag ccctgcgtat attgcggctc gcggatgcgg
    29101 acccctttcc cctcttcacg cactcttgca ggtattgacc cttgacgcca gggtaagcac
    29161 atggcgtttg ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc ttgttacccg
    29221 tagccgggta tttagcccgt ggactgcgtt ctacttttta cagtcgctat taattaacct
    29281 cggcttaggt taccccttca gtttgctcta caccgctgcg tttacggcta ttttgctttt
    29341 gctatggcga acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt cgctggtggc
    29401 ggcttgttat ttcccttttg ctcaggccta cggcgcgcct aatttcaata cattgctggc
    29461 attgcactcc accaatatgg aagagtcgac cgaaatcctg acgatttttc cgtggtacag
    29521 ctacctggtc ggcttattta tttttgcgct cggcgtaata gcaatcaggc gaaaaaaaga
    29581 gaatgaaaaa gcgcgctgga ataccttcga cagcctgtgt ctggtattca gtgtggcgac
    29641 attttttgtt gctcccgtgc aaaacctggc ctggggtggc gtatttaaac tgaaagatac
    29701 tggctatccg gtatttcgtt ttgctaagga tgtcatcgtc aataataacg aggtgattga
    29761 agagcaagaa cggatggcaa aactttccgg aatgaaagat acctggacgg tcactgccgt
    29821 taagccgaag tatcagacct atgtggtagt gatcggtgaa agcgcgcgtc gcgatgccct
    29881 cggtgccttt ggcggtcact gggacaatac cccgtttgcc agcagcgtta acggtttgat
    29941 atttgctgac tacattgctg ccagtggctc cacgcagaaa tcgcttggct taacgctcaa
    30001 tcgcgtagtc gatggcaaac cacagtttca ggataacttt gtcaccctgg caaatcgcgc
    30061 gggcttccag acctggtggt tttccaacca gggccaaatc ggcgaatacg atactgctat
    30121 cgccagcatc gccaaacgtg cagatgaagt gtacttcctg aaagaaggta attttgaagc
    30181 agataaaaac acgaaagacg aagcgttact ggatatgacc gctcaagtgc tggcgcaaga
    30241 gcactcgcaa ccgcagctga ttgttctgca tctgatgggc tcgcatccgc aggcctgcga
    30301 caggacacaa ggcaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt gctatctcta
    30361 taccatgacg caaacggacg atttactgcg caagctgtac gatcagttac gcaacagcgg
    30421 cagcagcttc tctctggttt acttttctga ccacggtctg gcctttaaag agcgcggcaa
    30481 agacgtgcaa taccttgccc atgatgataa atatcagcaa aatttccagg tgccttttat
    30541 ggtcatttcc agcggcgata aagcgcatcg ggtgattaaa gcccgccgct cagccaatga
    30601 cttcttaggc tttttctcgc agtggacggg aattaaagcg aaggaaataa atatcaaata
    30661 cccgtttata tctgagaaga aagccgggcc gatatacatc accaacttcc agttacagaa
    30721 ggtggattac aaccatctcg gaaccgatat tttcgacccg aaaccttaaa aacaaaaatc
    30781 cgccccgaga ggcggatttt tatatcacca aagtgattag aagcggtaac caacaccggc
    30841 aatccaggtg cctacgtcaa cgctacgaat acggctctgc tcgtaagaga agtccagagc
    30901 aacgttttcc atcgggttga actgcagacc agcaccgtag gagaaaccgt agtcgctggt
    30961 gtcgtgtttg taggtcgggt attcagtggt ctggaattta ccataaccca cacccactac
    31021 accgtagatg cttgcccagt cgttaatgcg gtaagccgga ccagcagtga tgccgtagta
    31081 ctggtttttg ttgtagtcac cagagcttgc agtacggctt ttctcggtgt aagtgaaaga
    31141 accgatcaca cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc
    31201 cattttgttc atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt
    31261 cgcagctacg gaagtacctg cggtgaaagc cagaactgcg gccagtgctg aaagacatgc
    31321 aatttttttc ataaccacct caaatgtgat tcaaataagt cctaagtttt aaatatatca
    31381 aaaattaatg ggaaactctt cgcgatttgt gatgtctaac gggccatttc atgtaacaga
    31441 acgtttccat acaccgctat ccatctaaat ttaaatcact ttttcagaga actgcgtaag
    31501 tattacgcat gttttccctg tcattcatcc agattattcc taatcaccag actaatgatt
    31561 ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat tttttcaaca aatgctcaac
    31621 cagcattggg tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga
    31681 aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca tattgctcgt
    31741 tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtgag
    31801 cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt
    31861 taagccatgg cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt
    31921 ttcgctgggt gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat
    31981 tgcggtggcg ctggagttca ccggaccact ggcggtggca ctgttctctt ctcgtcgccc
    32041 ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg
    32101 gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg
    32161 ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt
    32221 agcaattggt tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg
    32281 tgaagcactc tggcactggt cggttattcc attgggtctg gctgtcgcta ttctctcgac
    32341 cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg
    32401 tacgctgatg agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga
    32461 aacactgaca cccatacagc tgctggcgct cggcgctatc atcgccgctt caatggggtc
    32521 tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat
    32581 ttctgtatgg ttatgcataa ccatgcagaa tttctcgcta cttttcctct acaccgtatt
    32641 tatatatcga attatgcaaa agcatattta ttccgaaaat tcctggcgag cagataaata
    32701 agaattgttc ttatcaatat atctaactca ttgaatcttt attagttttg tttttcacgc
    32761 ttgttaccac tattagtgtg ataggaacag ccagaatagc ggaacacata gccggtgcta
    32821 tacttagtct cgttaattac tgggacataa catcaagagg atatgaaatt atgagtaccg
    32881 ctaaattagt taaatcaaaa gcgaccaatc tgctttatac ccgcaacgat gtctccgaca
    32941 gcgagaaaaa agcaacagta gagttgctga atcgccaggt tatccagttt attgatcttt
    33001 ctttgattac caaacaagcg cactggaaca tgcgcggcgc taacttcatt gccgtacatg
    33061 aaatgctgga tggcttccgc accgcactga tcgatcatct ggataccatg gcagaacgtg
    33121 ccgtgcagct gggcggtgta gctctgggga ccactcaagt tatcaacagc aaaaccccgc
    33181 tgaaaagtta cccgctggac atccacaacg ttcaggatca cctgaaagaa ctggctgacc
    33241 gttacgcaat cgtcgctaat gacgtacgca aagcgattgg cgaagcgaaa gatgacgaca
    33301 ccgcagatat cctgaccgcc gcgtctcgcg acctggataa attcctgtgg tttatcgagt
    33361 ctaacatcga ataaatccat cgctgatggt gcagaacttt agtacccgat aaaagcggct
    33421 tcctgacagg aggccgtttt gttttgcagc ccacctcaac gcacttattt agtgcatcca
    33481 tctgctatcc ccagctgatt aagtaaattt tttgtatcca catcatcaca caatcgttac
    33541 ataaagattg ttttttcatc aggttttacg ctaaataatc actgtgttga gtgcacaatt
    33601 ttagcgcacc agattggtgc cccagaatgg tgcatcttca gggtattgcc ctataaatcg
    33661 tgcatcacgt ttttgccgca tctcgaaaaa tcaatgagtt gcaaaattgg cacgattttt
    33721 tcatatatgt gaatgtcacg caggggatcg tcccgtggat agaaaaaagg aaatgctatg
    33781 aagtctgtat taaaagtttc actggctgca ctgaccctgg cttttgcggt ttcttctcat
    33841 gccgcggata aaaaattagt tgtcgcgacg gataccgcct tcgttccgtt tgaatttaaa
    33901 cagggcgata aatatgtggg ctttgacgtt gatctgtggg ctgccatcgc taaagagctg
    33961 aagctggatt acgaactgaa gccgatggat ttcagtggga tcattccggc actgcaaacc
    34021 aaaaacgtcg atctggcgct ggcgggcatt accatcaccg acgagcgtaa aaaagcgatc
    34081 gatttctctg acggctacta caaaagcggc ctgttagtga tggtgaaagc taacaataac
    34141 gatgtgaaaa gcgtgaaaga tctcgacggg aaagtggttg ctgtgaagag cggtactggc
    34201 tccgttgatt acgcgaaagc aaacatcaaa actaaagatc tgcgtcagtt cccgaacatc
    34261 gataacgcct atatggaact gggcaccaac cgcgcagacg ccgttctgca cgatacgcca
    34321 aacattctgt acttcatcaa aaccgccggt aacggtcagt tcaaagcggt aggtgactct
    34381 ctggaagcgc agcaatacgg tattgcgttc ccgaaaggta gcgacgagct gcgtgacaaa
    34441 gtcaacggcg cgttgaaaac cctgcgcgag aacggaactt acaacgaaat ctacaaaaaa
    34501 tggttcagta ctgaaccgaa ataataacgc tacacctgta aagcgcactg gcagttccct
    34561 ctcccccctg gggagaggat tagggtgagg ggcgcaaacc cgctccgggg ccattaatta
    34621 ccctgaattt gattatttac accacggtaa caggaacaac atatgcagtt tgactggagt
    34681 gccatctggc ctgccattcc gcttctgatt gaaggtgcca aaatgaccct gtggatttcg
    34741 gtcctcggtc tggctggcgg tctggtaatc ggattgctgg caggttttgc acgcaccttc
    34801 ggtggttgga tagccaacca cgtcgcgctg gtctttattg aagtgatccg cggcacacct
    34861 atcgtcgtcc aggtgatgtt tatctatttc gccctgccga tggcgtttaa cgacttacgc
    34921 atcgacccat ttactgcggc ggtggtcacc atcatgatca actccggcgc gtacattgcg
    34981 gaaatcacgc gtggtgcggt gctgtctatc cacaaaggtt ttcgtgaagc aggactggcg
    35041 ctcggtcttt cacgttggga aaccattcgc tacgtcattt taccgctggc actgcgtcgt
    35101 atgctgccgc cgctgggtaa ccagtggatc atcagcatta aagacacctc gctgtttatt
    35161 gttatcggcg tggcggaact gacccgtcag gggcaagaaa ttattgccgg taacttccgc
    35221 gcccttgaga tctggagcgc cgtggcggtg ttctatctga ttattaccct ggtgctgagc
    35281 tttattctgc gtcgtctgga aagaaggatg aaaatcctgt gattgaattt aaaaacgtct
    35341 ccaagcactt tggcccaacc caggtgctgc acaatatcga tttgaacatt gcccagggcg
    35401 aagtcgtggt gattatcggg ccgtccggtt ccggtaaatc gaccctgctg cgctgcatca
    35461 acaaactgga agaaatcacc tccggcgatc tgattgtcga tggcctgaag gtcaacgatc
    35521 cgaaagttga cgagcgcctg attcgccagg aagcaggtat ggtgttccag cagttttacc
    35581 tcttcccgca tctgacggcg ctgggaaacg tcatgtttgg tccgctacgc gtgcgtggcg
    35641 cgaacaaaga agaggcggaa aaactggcgc gtgagctgct ggcgaaagtc ggtctggcag
    35701 aacgtgcaca tcactaccct tccgaacttt ctggtggtca acagcagcgt gtggcgattg
    35761 cccgcgcgct ggcggtgaag ccgaaaatga tgctgtttga tgaaccgact tccgctcttg
    35821 acccggaact gcgccatgaa gtgctgaagg ttatgcagga tctggctgaa gaagggatga
    35881 cgatggtgat cgtgacccac gaaatcggtt ttgccgagaa agtagcttcg cggctgatct
    35941 ttatcgacaa aggccggatt gcggaagatg gcaatccgca ggtgttgatc aagaacccgc
    36001 cgagccagcg cttgcaggaa tttttgcagc acgtctctta ataagacaca ttgcctgatg
    36061 cgctacgctt atcaggccta caggatatct ggcaacttat taaaattgca tgaacttgta
    36121 ggacggataa ggcgttcacg ccgcatccgg caaaaaagcc cgcacgttgt cagcaacctg
    36181 cttaatatcc cttcctccct ttcacccgaa agggaggcac accagattcc tctcatttaa
    36241 aatcgcccct cccccagcat ctatacttat ctttttgctc tgttttctca ctggaggagt
    36301 catgcggtgg atcctgttca tcctcttctg cctgctgggc gcacctgccc acgcggtatc
    36361 catacccggc gttacaacca caacgacaac ggactcaacg actgaaccgg ccccggaacc
    36421 ggatatcgaa caaaaaaagc ggcctatggc gcactggcgg atgtgctgga taacgacacc
    36481 tcgcgtaaag agttgatcga ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg
    36541 gtcccgaaga tcgtgccgcc gacactggtt gaagagcaaa ccgtgctgca aaaggtcacc
    36601 gaagtcagcc gccattatgg tgaagccctt tccgcccgct tcgggcaact ttatcgcaat
    36661 atcaccggct ccccgcataa gccatttaat ccacaaacct tcagcaatgc gctgagccat
    36721 ttttcaatgt tagcggtatt agtgtttggt ttttactggc tgattcgcct gtgcgcactg
    36781 ccgctatatc gcaaaatggg tcagtgggcg aggcaaaaaa atcgtgagcg cagcaactgg
    36841 ttgcagcttc cggcgatgat tattgggacg tttattatcg acctgctgtt actggccctg
    36901 acattatttg tcggccaggt attaagcgac aacctgaatg caggcagtcg caccatcgct
    36961 ttccaacaaa gtttgtttct caacgccttt gccctgattg aatttttcaa agccgtacta
    37021 cgcctgattt tttgcccaaa cgtggcggag ctgcgcccgt tcacgattca ggacgagagc
    37081 gcccgttact ggggtcgtcg cctgagctgg ttaagcagcc tgattggcta tggcctgatt
    37141 gtggccgtgc cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc
    37201 atcattatgc tgtgcatgac cgtctgggcg ttgtacctga tcttccgaaa taaaaaagag
    37261 attacccagc atatgctcaa ctttgcggag cattcgctgg cttttttcag cctgtttatc
    37321 cgcgcctttg cgctggtgtg gcactggctg gcaagcgcct attttatcat gctgtttttc
    37381 ttttcgttgt tcgatccggg caacagtctg aaatttatga tgggtgcaac ggtgcgcagc
    37441 ctggcgatta ttggtatcgc agcgtttgtt tccggtatgt tttcccgctg gctggcgaaa
    37501 accatcaccc tctcgccaca tactcagcgt aactatccgg agctacaaaa acggctgaat
    37561 ggctggcttt cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcagtaatg
    37621 ttgctgttga gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg
    37681 cagaaaaccg tagatatcct gatccgcatc gcactcattc ttttcttctc ggcggttggc
    37741 tggacggtgc tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg
    37801 ctacccagcg cccgtacgcg taccctgctg acgctgttcc gtaacgcgct ggcggtgatt
    37861 atcagtacca tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg
    37921 ctggcaggtg ccggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtaaaa
    37981 gatattatca ccggggtatt tattcagttt gaaaacggca tgaacactgg agatttggtg
    38041 actatcgggc cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc
    38101 caggataccg gggcgtatca catcattccg tggtcttcga taaccacctt tgctaacttc
    38161 gtccacggta ttggttcggt agtggcaaac tacgatgttg atcgccatga agatgctgat
    38221 aaagccaatc aggcactgaa agatgcggta acggaattga tggaaaacga agaaattcgc
    38281 gggctgatta ttggtgaacc gaactttgcc gggattgtcg gcttaagcaa taccgcgttt
    38341 acactgcatg tttcgttcac cacgctgcca ctcaaacagt ggacggtacg ctttgccctc
    38401 gacagccagg tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt gcagacttat
    38461 caggtgctgc ctgctccggg cgcgaccccg gctgaaccgc tgccgccggg ggagccaacg
    38521 ctttaacgtt ggcgattaac aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca
    38581 ataaagcgac tctgcttttg cccctgggcc atctcttttt ttaccacctt caccgcgccc
    38641 acgtcggtca gagcacgata caacggcggt aagttttcac cacgagatac cagcgaggta
    38701 aaccacatca cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt gataaagttg
    38761 acttcaccgc cttcacacca caactcctgt tgctgaccac caaagttcag tgcatcgtct
    38821 ttgttcagcc ccaggttacg gcgtttacgc tcactacctg cccgcgccgc agcggcggaa
    38881 tcgtggaatg gcgggttaca caaggtcgcg tcgtattgct cgtttttgtg gatgatgccg
    38941 ttaaaaatcg ccccactctc tttttgccga cgcagacgaa tggcgcggtt aagccccgga
    39001 ttagcactga taatcgcctg cgcactgctt aacgcctggc tgctggtttc actaccggta
    39061 aaacgccagc catattcatg tacgccaatc agcggataaa tacagttcgc accaacgccg
    39121 atatccagaa tgctggcatt cgctggaatt gttccgcttg cttcagccag taaatcggca
    39181 aggtgatgaa tataatccgc ccggcccggt actggtgggc agagaaaacc gtcggggata
    39241 tcccagttcg ctacggcgta aaaatgggcc agcaacgcct tattgagcgc cttcaccgcc
    39301 agcggattgg caaagtctac gctttgctcc cctgcgggtg taagcgtgag gaattgcctg
    39361 agttcaggat tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc
    39421 ggatgcaacc ccggtttctg ggcggacata gcattctcct ttaagcagcg gcgtaagata
    39481 cccgcataac gcgacagggt aaatatctgg ctgcgtttat cattccccaa aaccttaaca
    39541 tggctatggt ttatggcaat tcatcataaa aatcagcatt atgcatgtca ttgatttgtc
    39601 ttattttgta aaaatatatc gttcaaaaaa taagcatatc tctgtcaatt ttttgcgtca
    39661 tttgcatcgc gcaggcgacc tacacttatc aggcactacc cgttgtattt atgaggatgt
    39721 cattatgaaa aagtgcctca cactactgat tgccaccgtc ctgagcggaa tctctctcac
    39781 ggcttatgcc gcgcaaccga tgagtaacct ggacagcggt cagctacggc ccgccggtac
    39841 cgtttcggca actggcgcat caaacctaag cgatctggag gataaactgg cagaaaaagc
    39901 gcgcgaacaa ggcgcgaaag gttatgtcat taattccgca ggcggaaatg accagatgtt
    39961 aggtactgca accatctaca aataacccca ccaacgtaac tgcattcgcc tctggatgca
    40021 gttttcgcct gttttgtaat caaaaaacat caaatatgaa cgcaatgtaa ataaatgtat
    40081 ttctttttcg cgcaatgggt gatagaaaat cgcatcaagt gataatgctt atcaaaatta
    40141 ttatcacttg cacgaacact atcacgggat taacagtggc atcgcatccg cagagatgct
    40201 ttctcgtggc agtgaaaatt tcaacatata agaaaaagtc acctgcaaaa tggaaaacaa
    40261 tcgcaatttc cctgccagac aatttcattc gctcacgttc tttgccggtc tttgtattgg
    40321 tattacgcct gtggcacagg cactcgccgc cgaagggcaa gctaacgcgg atgacacgct
    40381 ggttgtcgaa gcatcaacgc cttcgcttta tgcgccacaa caatctgccg atccgaaatt
    40441 ctcgcgtccg gtagcggata ctacccgcac gatgacggtg atttctgaac aagtgattaa
    40501 agatcagggc gcaaccaacc ttaccgacgc gctcaaaaac gtccccggcg tgggtaatgc
    40561 cagtcagtta agcaactgac tggctctttt tcggggc
//
LOCUS       DAOFMX010000032        39762 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.032, whole genome shotgun sequence.
ACCESSION   DAOFMX010000032 DAOFMX010000000
VERSION     DAOFMX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.032"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..156
                     /locus_tag="QBA20_001868"
     CDS             <1..156
                     /locus_tag="QBA20_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ6292423.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            385..975
                     /gene="uidR"
                     /locus_tag="QBA20_001869"
     CDS             385..975
                     /gene="uidR"
                     /locus_tag="QBA20_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDQ6292424.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1363..3066
                     /gene="uidA"
                     /locus_tag="QBA20_001870"
     CDS             1363..3066
                     /gene="uidA"
                     /locus_tag="QBA20_001870"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDQ6292425.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3072..4097
                     /gene="uidB"
                     /locus_tag="QBA20_001871"
                     /pseudo
     CDS             3072..4097
                     /gene="uidB"
                     /locus_tag="QBA20_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4136..5401
                     /gene="uidC"
                     /locus_tag="QBA20_001872"
     CDS             4136..5401
                     /gene="uidC"
                     /locus_tag="QBA20_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDQ6292426.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5627..7135)
                     /locus_tag="QBA20_001873"
     CDS             complement(5627..7135)
                     /locus_tag="QBA20_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDQ6292427.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7236..8411)
                     /gene="manA"
                     /locus_tag="QBA20_001874"
     CDS             complement(7236..8411)
                     /gene="manA"
                     /locus_tag="QBA20_001874"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDQ6292428.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8610..10256
                     /gene="fumB"
                     /locus_tag="QBA20_001875"
     CDS             8610..10256
                     /gene="fumB"
                     /locus_tag="QBA20_001875"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDQ6292429.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10399..11802
                     /gene="fumC"
                     /locus_tag="QBA20_001876"
     CDS             10399..11802
                     /gene="fumC"
                     /locus_tag="QBA20_001876"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDQ6292430.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11799..12728)
                     /gene="tus"
                     /locus_tag="QBA20_001877"
     CDS             complement(11799..12728)
                     /gene="tus"
                     /locus_tag="QBA20_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDQ6292431.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12804..14105)
                     /gene="rstB"
                     /locus_tag="QBA20_001878"
     CDS             complement(12804..14105)
                     /gene="rstB"
                     /locus_tag="QBA20_001878"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDQ6292432.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVSPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14284..15603)
                     /locus_tag="QBA20_001879"
     CDS             complement(14284..15603)
                     /locus_tag="QBA20_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDQ6292433.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15828..16571)
                     /gene="rstA"
                     /locus_tag="QBA20_001880"
     CDS             complement(15828..16571)
                     /gene="rstA"
                     /locus_tag="QBA20_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDQ6292434.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16700..17035
                     /locus_tag="QBA20_001881"
     CDS             16700..17035
                     /locus_tag="QBA20_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDQ6292435.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASKRGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17032..17754)
                     /gene="folM"
                     /locus_tag="QBA20_001882"
     CDS             complement(17032..17754)
                     /gene="folM"
                     /locus_tag="QBA20_001882"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDQ6292436.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17791..19173)
                     /locus_tag="QBA20_001883"
     CDS             complement(17791..19173)
                     /locus_tag="QBA20_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ6292437.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19359..20303)
                     /locus_tag="QBA20_001884"
     CDS             complement(19359..20303)
                     /locus_tag="QBA20_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ6292438.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20827..22359
                     /gene="pntA"
                     /locus_tag="QBA20_001885"
     CDS             20827..22359
                     /gene="pntA"
                     /locus_tag="QBA20_001885"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDQ6292439.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22370..23758
                     /gene="pntB"
                     /locus_tag="QBA20_001886"
     CDS             22370..23758
                     /gene="pntB"
                     /locus_tag="QBA20_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDQ6292440.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23783..24817)
                     /gene="tqsA"
                     /locus_tag="QBA20_001887"
     CDS             complement(23783..24817)
                     /gene="tqsA"
                     /locus_tag="QBA20_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDQ6292441.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25228..25593
                     /gene="mdtJ"
                     /locus_tag="QBA20_001888"
     CDS             25228..25593
                     /gene="mdtJ"
                     /locus_tag="QBA20_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDQ6292442.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25580..25909
                     /gene="mdtI"
                     /locus_tag="QBA20_001889"
     CDS             25580..25909
                     /gene="mdtI"
                     /locus_tag="QBA20_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDQ6292443.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25948..26769)
                     /locus_tag="QBA20_001890"
     CDS             complement(25948..26769)
                     /locus_tag="QBA20_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDQ6292444.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26869..26952)
                     /locus_tag="QBA20_001891"
     CDS             complement(26869..26952)
                     /locus_tag="QBA20_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292445.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27045..27380)
                     /locus_tag="QBA20_001892"
     CDS             complement(27045..27380)
                     /locus_tag="QBA20_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDQ6292446.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27777..29030)
                     /locus_tag="QBA20_001893"
                     /pseudo
     CDS             complement(27777..29030)
                     /locus_tag="QBA20_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29137..30057
                     /locus_tag="QBA20_001894"
     CDS             29137..30057
                     /locus_tag="QBA20_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6292447.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAELAKEPFVFFDPHVGTGLYDDI
                     LGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDA
                     VSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30192..31412
                     /gene="mlc"
                     /locus_tag="QBA20_001895"
                     /gene_synonym="dgsA"
     CDS             30192..31412
                     /gene="mlc"
                     /locus_tag="QBA20_001895"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDQ6292448.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31537..32232
                     /gene="bioD"
                     /locus_tag="QBA20_001896"
     CDS             31537..32232
                     /gene="bioD"
                     /locus_tag="QBA20_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDQ6292449.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32185..33477)
                     /gene="clcB"
                     /locus_tag="QBA20_001897"
     CDS             complement(32185..33477)
                     /gene="clcB"
                     /locus_tag="QBA20_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDQ6292450.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33636..34250)
                     /gene="dmsD"
                     /locus_tag="QBA20_001898"
     CDS             complement(33636..34250)
                     /gene="dmsD"
                     /locus_tag="QBA20_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDQ6292451.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34293..35147)
                     /locus_tag="QBA20_001899"
     CDS             complement(34293..35147)
                     /locus_tag="QBA20_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDQ6292452.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35149..35766)
                     /gene="dmsB"
                     /locus_tag="QBA20_001900"
     CDS             complement(35149..35766)
                     /gene="dmsB"
                     /locus_tag="QBA20_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDQ6292453.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35777..37815)
                     /locus_tag="QBA20_001901"
                     /pseudo
     CDS             complement(35777..37815)
                     /locus_tag="QBA20_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37876..38985)
                     /locus_tag="QBA20_001902"
     CDS             complement(37876..38985)
                     /locus_tag="QBA20_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDQ6292454.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38968..>39762
                     /locus_tag="QBA20_001903"
     CDS             38968..>39762
                     /locus_tag="QBA20_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDQ6292455.1"
                     /translation="MTDINHDDTAYPSSSFSGSPYLYQQVYTSSPDTRLTQIKDLATP
                     QLVRRTNGRYTVRFRLKGATSPFLSVSTRSTDRRTATMRQRELAATAKAFLLDNPEVT
                     LQELTAHIRSMAEQFLTDASDDYWNGVEVATLVDEKSNLRELAATRALSVDQQRGIKL
                     ALEVLTAAQQRVDTGDTSGLIKLIDGGVSQLTDDNNHTDYSTIGDSTSILKNEQGDRP
                     AVFTQERQLLTPESRESSVLFQELVTSLLAEKVQTLKQSSFKDLSAS"
BASE COUNT        10122 a         9642 c        10177 g         9821 t
ORIGIN      
        1 attgaacaaa aaatgttaca gcacacgccg atcagacgtc tgggccaacc gcaagatatt
       61 gctaacgcgg cgctgttcct ttgctcgcct gcagccagct gggtaagcgg acaaattctc
      121 accgtctccg gtggtggggt acaggagctc aattaataca ctaacgaacc ggtaaacggt
      181 cgtatgtgtt gtttaccggg atagcgtgtg caattcatca acgtctctgc taaacaactg
      241 gcagccaaat cacggctatt ggttaaccaa ttgctgggtg aaaagtatac gaatagagtg
      301 taccttcgca ctattcaaca gcaatgatag gcgctcacct gacaacgcgg taaactagtt
      361 attcacgcta actataatgg tttaatgatg gataacatgc agactgaagc acaaccgaca
      421 cggacccgga tcctcaatgc tgccagagag attttttcag aaaatggatt tcacagtgcc
      481 tcgatgaaag ccatctgtaa atcttgcgcc attagtcctg ggacgctcta tcaccatttc
      541 atctccaaag aagccttgat tcaggcgatt atcttacagg accaggagag ggcgctggct
      601 cgtttccggg aaccgattga agggattcat tttgttgact atatggtcga gtctattgtc
      661 tctctcaccc atgaagcctt tgggcaacgg gcgctggtgg tcgaaattat ggcggaaggg
      721 atgcgtaacc cacaggtcgc cgccatgctt aaaaataagc atatgacgat cactgaattt
      781 gttgcccagc ggatgcgtga tgcccagcaa aaaggcgaga taagcccaga catcaacacg
      841 gcaatgactt cacgtttact gctggatctg acctacggtg tactggccga tatcgaagcg
      901 gaagacctgg cgcgtgaagc gtcgtttgct cagggattac gcgcgatgat tggcggtata
      961 ttaaccgcat cctgatcctc tcatttcgcg ggctggtgat aactgtgccc gcgtttcata
     1021 tcgtaatttc tctgtgcaaa aattatcctc cccgtgttcg gagaattccc ccccaaaata
     1081 ttcaccgtag ccatatgtca tgagagttta tcgttcccaa cactcttaaa cgaacgttcg
     1141 attgcttatt ttatggcttc tgtcaacgct gttttaaaga ttaatgcgat ccatatcacg
     1201 ctgtgggtat tgcagttttt ggttttttga tcgcggtgtc agttcttttt atttccattt
     1261 ctcttccatg ggtttctcac agataactgt gtgcaacaca gaattggtta actaatcaga
     1321 ttaaaggttg accagtattt ttatcttaat gaggagtccc ttatgttacg tcctgtagaa
     1381 accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa
     1441 aactgtggaa ttgatcagcg ttggtgcgaa agcgcgttac aagaaagccg ggcaattgca
     1501 gtgccaggca gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgtgggcaac
     1561 gtctggtatc agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg
     1621 cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag
     1681 catcagggcg gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa
     1741 agtgtacgta tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga
     1801 atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac
     1861 tatgccggga tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat
     1921 atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg
     1981 gtggccaatg gtgatatcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact
     2041 ggacaaggca ccagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa
     2101 ggttatctct atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg
     2161 ctgcgcgtcg gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa
     2221 ccgttctact ttactggctt tggtcgtcat gaagatgcgg atttgcgagg caaaggattc
     2281 gataacgtgc tgatggtgca cgatcacgca ttaatggact ggattggggc caactcctac
     2341 cgtacctcgc attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc
     2401 gtggtgattg atgaaactgc agctgtcggc tttaacctct ctttaggcat tggtttcgaa
     2461 gcgggcaaca agccgaaaga actgtacagc gaagaggctg tcaacgggga aactcagcag
     2521 gcgcacttac aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg
     2581 atgtggagta ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg
     2641 ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta
     2701 atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac
     2761 cgttattacg gttggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa
     2821 aaagaactgc tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc
     2881 gtggatacgt tagccggcct gcactcaatg tacaccgaca tgtggagtga agagtatcag
     2941 tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa
     3001 caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac
     3061 aattgaccta catgggcctc gggctttgct acagcctggt gaatattcct tatggttcac
     3121 ttgctaccgc gatgacccaa caaccacaat ctcgcgcccg tctgggcgcg gctcgtggga
     3181 ttgccgcttc attgaccttt gtatgcctgg catttctgat aggaccgagc attaagaact
     3241 ccagcccgga agagatggtg tcggtatacc atttctggac gattgtgctg gtgattgccg
     3301 gaatggtgct ttacttcatc tgcttcaaat cgacgcgtga aaatgtggta cgtatcgtgg
     3361 cgcagccgtc attgaagatc agtttgcaaa ccctgaaacg gaatcgcccg ctgtttatgt
     3421 tgtgcatcgg tgcgctgtgt gtgctgattt cgaccttcgc ggtcagcgcc tcgtcgttgt
     3481 tctacgtgcg ctatgtgtta aatgataccg ggctgttcac tgtgctggta ctggtgcaaa
     3541 acctggttgg tactgtggca tcggcaccgc ttgtgccggg gatggtcgcg aggatcggta
     3601 aaaagaatac cttcctgatt ggcgctttgc tgggaacctg cggttatctg ctattcttct
     3661 gggtttccgt ctggtcactg ccggtggcgt tggttgcgtt ggccatcgct tcaattggtc
     3721 agggcgttac tatgactgtg atgtgggcac tggaagctga taccgtagaa tacggtgaat
     3781 acctgaccgg cgtgcgaatt gaagggctca cctattcact attctcattt acccgtaaat
     3841 gcggtcaggc aatcggaggt tcaattcctg cctttatttt gggattaagc ggatatatcg
     3901 ccaatcaggt gtaaacgccg gaagttatta tgggcatccg cacatcaatt gccttagtac
     3961 cttgcggatt tatgctactg gcattcgtta ttatctggtt ttatccgctc acggataaaa
     4021 aattcaaaga aatcgtggtt gaaattgata atcgtaaaaa agtgcagcag caattaatca
     4081 gcgatatcac taattaatat tcaataaaaa taatcagaac atcaaaggtg caactatgag
     4141 aaaaatagtg gccatggccg ttatttgcct gacggctgcc tctggcctta cctctgctta
     4201 cgcggcgcaa ctggctgacg atgaagcggg actacgcatc agactgaaaa acgaattgcg
     4261 cagggctgat aagcccagtg ctggcgcggg aagagatatt tacgcatggg tacagggagg
     4321 attgctcgat ttcaatagtg gttattattc caatattgtt ggcgttgaag gcggagcgta
     4381 ttatgtttat aaattaggtg ctcgtgctga tatgagtacc cggtggtatc ttgatggtga
     4441 taaaagtttt ggctttgccc tgggggcagt aaaaataaaa cccagtgaaa atagcctgct
     4501 taaattaggt cgcttcggga cggattatag ttatggtagc ttaccttatc gtattccgtt
     4561 aatggttggc agttcgcaac gtacattacc gacagtttct gaaggagcat taggttattg
     4621 ggctttaacg ccaaatattg atctgtgggg aatgtggcgt tcacgagtat ttttatggac
     4681 tgattcaaca accggtattc gtgatgaagg ggtgtataac agccagacgg gaaaatacga
     4741 taaacatcgc gcacgttctt ttttagccgc cagttggcat gatgatacca gtcgctattc
     4801 tctgggggca tcggtacaga aagatgtttc caatcagata caaagtattc tcgagaaaag
     4861 cataccgctc gacccgaatt atacgttgaa aggggagttg ctcggctttt acgcgcagct
     4921 cgaaggttta agtcgtaata ccagccagcc caatgaaacg gcgttggtta gtggacaatt
     4981 gacctggaat gcgccgtggg gaagcgtatt tggcagtggt ggttatttgc gccatgcaat
     5041 gaatggtgcc gtggtggata ccgacattgg ctatcccttt tcattaagtc ttgatcgtaa
     5101 ccgtgaagga atgcagtcct ggcaattagg tgccaactac cgtgtaactc cgcaatttac
     5161 gctgacattt gcaccgattg tgactcgcgg ctatgaatcc agtaaacgag atgtgcggat
     5221 tgaaggcgcg ggtatcttag gtggtatgaa ctatcgggtc agcgaagggc cgttacaagg
     5281 gatgaatttc tttcttgctg ccgataaagg gcgggaaaag cgcgatggca gtacgctggg
     5341 cgatcgcctg aattactggg atgtgaaaat gagtattcag tatgacttta tgctgaagta
     5401 agaaataacg ccggagagaa aaacctccgg cgtttcagat tgttgacaaa gtgcgcgttt
     5461 tttatgccgg atgcggcgta aacgccttat ccagcctaca aaaactcata aattcaaagt
     5521 gttgcaggaa aaggtaggcc tgataagcgt agcgcatcag gcaatctctg gtttgttttc
     5581 agatgaaaac gccggagtga aaattctccg gcgttttggc cgtgaattac tgctgcggaa
     5641 ttgccggtac agccggaacg ttaagagctg gcatcgcaaa catgccaaca aaatcttcta
     5701 acgacatttt ctgcccattt aacgttatct gaccgttagc atattgcagg ctggtggtga
     5761 tggtattgtc ctgcaaggtg gtcagacgga acatctgccc cattgctgat gcaccttcaa
     5821 cttgctgttt cgccagtttt ttcgcttgat cttcctgata accttccagc ttcgctacct
     5881 gagtcatcaa ctcagttgcc atatccaccg gaatggtcag tttcgcatcc agagatttaa
     5941 ccgaacgatc tacttcctgc gccagcgttt gcggcgcttc tttagtcgtt gccggatctt
     6001 tcaggaacag cgacagattc agcgcacttt caccctgact gtttttccag cttagcggcg
     6061 cgatagtaat caccggatcg cctttcaaca tcagcggcag ggcgctaaag aaggcttccg
     6121 tcactttctc ctgataaagt tcgggattgt tggcaatttc tggctgtgcc agcagcgcct
     6181 gagtttgcgc gttatattgc tggctaaact gatgccaggc ttcaccatca atctggccga
     6241 cttttaaagt cagcttgccg ctgcccagat cctgattctg taccttcagg ctgtttagcg
     6301 agtaatccag ttggctattg atcgttttac cgtcattgac cagatccgat ttaccgctga
     6361 tctccatgcc ttccagcagt gccagttctt tgccttccac tgaaatggtc attttttcca
     6421 gtgacagttt ttggtttcct acgcgctcac caaaacttcc cagcgtgctg gaaccgtcgg
     6481 ttttcagatt attaaaggtc aactgcactt tctggttgta ttcgttaact gcgtctatcc
     6541 gaccactttg cgcctctccg gaaagggaga tggctttgcc gtctctgtca gcatttaact
     6601 ggaactcgcc gccgctaaag gcgacttttt catccttttg ctcgtaattc agtggattga
     6661 gcgaaatatc ggaactggaa tcaccgctgt aaccaatgcg cgagttaatc tcaaaaggcg
     6721 tttcaccttt tgccatatca aacagtggtt tgcttacttc gttattaacc agcgtggttt
     6781 gaattgatgc catcgacggg atcaggttca gttttttaag ctgggcaagc gggaagggac
     6841 catgatcaac cgattcgttg aagatgacgc tctgaccgct tttaatccac ggattttctt
     6901 tcccggcaat gggtttcacc aacagttgca actggctgct gaatacgccg cgatgatagt
     6961 tttgataact cacttccagg ttggattcag gagctgtcag tttgagttgc gcgttcgcct
     7021 gcgcgaccat gtcttcgaga tgggtttcaa tcttcttgcc tgtataccat gcgccgcctg
     7081 tccagactac gcctagcgca acaatgacgc ctaccgctac cagcgattta ttcataatga
     7141 ttatccataa aatgaaatca ggcggactgg ccgcctgaag gtgttataag cctttaataa
     7201 gcttagcaag agatgttaat tttttcagta agctcttaca gcttgttgta aacacgcgct
     7261 aaacggccgt ggcctttgac agtcaccggt gattcgttgg cggcaataaa cgctgattca
     7321 cccggtttaa gctgtaactg ctgagaacct ttccacaacg ttgcatcgcc ttcgacgcag
     7381 aacaaaatgg cggcactctg ctggctgatg gtggtttctt tatcactaag gtcatgcagc
     7441 gagaaggcaa aatcatccac aggaatcggg aagtccagtt ctgcaccttg tttcaccggc
     7501 tgggtcaaca actggttagc cggtttggct tcgaatttca cattggcaac cagttccgga
     7561 atatcaatgt atttaggcgt cagacctgca cgcagcacgt tatcggagtt cgccatcact
     7621 tccagcgcca cgccttgcag gtaagcgtgc ggtgtttcag cgaacaggaa catcgcttcg
     7681 ccaggattca atttcaccac attcagtaac agcggggaga acaggccgct gtcttccggg
     7741 taaaattcag aaattaaacg aatcgtttgc cacggttcac cctgctgact gtcgagcgcc
     7801 gattttaaaa tcgccagcgc gcgggatttt tcttcaccct gcatattcaa caggttggcg
     7861 aacagttcgc ttaaacgttc ggcatgtggc tgttgtaaaa agtgagcaat cgccggatgt
     7921 gcaccggcga ccggctggag tagggagaca atctcggaaa attcacgaaa cgcgttcatc
     7981 gcaaggaaag gcgtcagcgc aaaaaccagc tccggcttgt ggttaggatc tttatagtta
     8041 cgctcggcgg catccatcgg gatacctgcg gcattttctt tggcaaaacc gatttcagaa
     8101 ttgtgtttgt ttggatgaac ctgaatggag agtggctgtg ctgcgcataa tactttgaac
     8161 aggaaaggca gttcgccaaa gcgtttggca acggcctctc cgagcagagt cgatttatca
     8221 ctctcaatca catcacgcag tgaaacgata tctccggcgg cattctgcac tcgtgaactg
     8281 cttttcggat gtgcgcccat ccacagctcg gccatcggct ggctggacgg attttccata
     8341 ccataaagtt cagtcaacgc cgttttgcta ccccaggcat agttttgcac tgagttaatg
     8401 agtttttgca tgatcaatcc ctgttttaat gtggaaatta atcccgctat taaagcaaga
     8461 atcctacggg aagtaacctg gagccgcaaa aagtcgtact agtctcagtt tttgttaaaa
     8521 aactgtgtag gatattgtta ctcgctttta acagggcaac ggaacacccg cccagagcat
     8581 aaccaaacca ggcagtaagt gagagaacaa tgtcaaacaa accctttcat tatcagtctc
     8641 cttttccact caaaaaaaat gatactgagt attacctgct aaccagtgaa cacgttagcg
     8701 tatctgaatt tgaagggcag gagattttga aagtcgcacc cgaagcgtta actctgttgg
     8761 cgcgtcaggc gtttcatgat gcgtcgttta tgctgcgtcc ggctcaccaa caacaggtgg
     8821 ccgacattct gcgtgacccg gaggccagcg aaaatgataa atatgtggcg ctgcaattcc
     8881 tgcgtaactc cgacatcgcg gcgaaaggcg ttctgccaac ctgtcaggat accggcaccg
     8941 cgattattgt tggtaaaaaa gggcagcgtg tatggaccgg tggtggtgat gaagcggcgc
     9001 tggcgcgcgg tgtctataac acttatatcg aagataatct gcgctactcg caaaacgcgc
     9061 cgctggatat gtataaagaa gtgaataccg gcaccaatct gccagcgcag atcgatcttt
     9121 atgccgttga tggcgacgaa tacaaattcc tctgtatcgc aaaaggtggt ggttcggcaa
     9181 acaagacgta tctctatcag gaaaccaaag cgttactgac gccggggaaa ctgaaaaatt
     9241 acctggttga gaagatgcgc acgctgggta ccgcggcctg tcctccgtat catattgcgt
     9301 tcgttattgg tggaacttct gcagaaacga accttaaaac ggtgaaactg gcttccgcga
     9361 aatactatga tgaactgcca acggaaggga atgagcacgg tcaggcgttc cgcgatgtgg
     9421 aactggaaaa agaattactg atcgaagcgc aaaatctcgg tctgggtgcg cagtttggtg
     9481 gtaaatactt cgctcacgac atccgcgtga ttcgcctgcc acgtcacggc gcatcctgtc
     9541 cggttggtat gggtgtttcc tgctctgctg accgtaatat caaagcgaag atcaaccgtc
     9601 aggggatctg gatcgaaaaa ctggaacata atccaggtaa atatatccct gaagagctgc
     9661 gtaaagcggg agaaggcgaa gcggtgcgcg ttgaccttaa ccgtccgatg aaagagatcc
     9721 tcgcacagtt gtcgcagtat cccgtttcta cacgcttatc gcttaacggc acgattatcg
     9781 ttggtcgtga tattgctcac gccaaactga aagagcggat ggataacggt gaagggctgc
     9841 cgcaatacat taaagatcat ccgatttact acgcgggtcc ggccaaaacg ccggaaggtt
     9901 atgcctccgg ttctcttggc ccaacgaccg ccggacggat ggattcttat gtcgatcaac
     9961 tgcaagcgca gggcggaagt atgatcatgc tggcgaaagg caaccgcagc cagcaggtga
    10021 cggatgcctg taaaaaacac ggcggcttct accttggcag tatcggtggt ccggccgctg
    10081 tattggcgca gggaagtatt aagagcctgg aatgtgttga atatccggaa ctgggaatgg
    10141 aagccatctg gaaaattgaa gtggaagatt tcccggcgtt tatccttgtg gatgataaag
    10201 gaaatgactt cttccagcag atacaactca cacagtgcac tcgctgtgtg aaataaacag
    10261 agccgccctt cggggcggtt tttttacatg gcacgaaaga ccaaacattt gttatcaaat
    10321 agtaaataat gagtgagcta aaagttgctt aacgaaagca aaacagaaag aaaaaattaa
    10381 tcaggtgagg agcaggccat gaatacagta cgcagcgaaa aagattcgat gggggcgatt
    10441 gatgtcccgg cagataagct gtggggcgca caaactcaac gctcgctgga gcatttccgc
    10501 atttcgacgg agaaaatgcc cacctcactg attcatgcgc tggcgctaac caagcgcgca
    10561 gcggcaaaag ttaatgaaga tttagacttg ttgtctgaag agaaagcgag cgccattcgg
    10621 caggcggcgg atgaagtact ggcaggacag catgacgacg aattcccgct ggctatctgg
    10681 cagaccggct ccggcacgca aagtaacatg aacatgaacg aagtgctggc taaccgggcc
    10741 agtgaattac tcggcggtgt gcgcgggatg gaacgtaaag ttcaccctaa cgacgacgtg
    10801 aacaaaagcc aaagttccaa cgatgtcttt ccgacggcga tgcacgttgc ggcgctgctg
    10861 gcactgcgca agcaactcat tccgcagctt aaaaccctga cacagacgct gagtgaaaaa
    10921 tcgcgtgcat ttgccgatat cgtcaaaatc ggtcgaaccc acttgcagga cgccacgccg
    10981 ctaacactag ggcaggagat ttccggctgg gtagcgatgc tcgagcataa tctcaaacat
    11041 atcgaataca gcctgcctca cgtagcggaa ctggctcttg gcggtacagc ggtgggtact
    11101 ggactaaata cccatccgga gtatgcgcgt cgcgtagcag atgaactggc agtcattacc
    11161 tgtgcaccgt ttgttaccgc gccgaacaaa tttgaagcgc ttgcgacctg tgatgccctg
    11221 gttcaggcgc acggcgcgtt gaaagggttg gctgcgtcac tgatgaaaat cgccaatgat
    11281 gtccgctggc tggcctctgg cccgcgctgc ggaattggtg aaatctcaat cccggaaaat
    11341 gagccgggca gctcaatcat gccggggaaa gtgaacccaa cccagtgtga agcattaacc
    11401 atgctctgct gtcaggtaat ggggaacgac gtggcgatca acatgggtgg cgcttccggt
    11461 aactttgaac tgaacgtctt ccgtccgatg gtgatccata atttcctgca atcggtgcgc
    11521 ttgctggcag atggcatgga aagttttaac aaacactgcg cagtgggtat tgaaccgaat
    11581 cgtgagcgaa tcaatcaatt actcaatgaa tcgctgatgc tggtgactgc gcttaacacc
    11641 cacattggtt atgacaaagc cgcggagatc gccaaaaaag cgcataaaga agggctgacc
    11701 ttaaaagctg cggcccttgc gctggggtat cttagcgaag ccgagtttga cagctgggta
    11761 cggccagaac agatggtcgg cagtatgaaa gccgggcgtt aatctgcaac atacaggtgc
    11821 agccgtggaa tgatcaaacg aagcggctgc gcctgaggtt tataacggtg ctgcacattg
    11881 tcggcatcgt aatttaacaa ctcaccaacg tccggcacgc ccgcgccatt atcccgatta
    11941 atcagtgcaa tcagtggtgt agggcaggcg tgttggactt gtttttgatc tcctttgtac
    12001 cagacgcggg caatcggctg caccttcact ggacgtttga tttttaactt cgcgttctgt
    12061 ggcagggcag cgatatcctg atactctcgc tccagttttc tttgccactc ctcgcgcgtc
    12121 cacggtgcga cactgcgtgg tgatttcagg cttttttcca gctgtgccag gacttcatca
    12181 cgatgcaaat tcttaatgat atgtttatta gcccaaccaa agcgtaaagt ggcggggtcg
    12241 tgcagaacgg tgagcgtgcg gtaagcatta agggtgatca gccccggcaa atgacgatgc
    12301 acccattcaa aacgtgccgc ggtggggagt tctgattcaa ccgtgacgat atgctcgaac
    12361 gtggtcttga gtttattgat gtgctgaata tgactgacca acgctgcttg cgaaaggtta
    12421 tcgacctggt aacacaacac gccaggcaga cggacagcgg ccttgctgct gcgattttcg
    12481 gactgttgtt gaataaataa atggcggaaa tgacgcaacg ccagcgactg cgcgtcgttg
    12541 ccgagatgtt gttttacctc aatacgatta agcggattat gctcatcctc tttttttacc
    12601 tccggcaaag agaacacgcg ggcaaccaat agcttgtgtt gctcaagatg agcggcaaat
    12661 gtagccagct cttgttccat ctggcgaaag gtagtgttga gtcggtctac gagatcgtaa
    12721 cgcgccataa tattaaccac tttagttaca acatacttat tttatagcac agtcgtggtg
    12781 acttgaccat cgtgcgcgta gtgtcaggca gaggtaaatt gcgggatgtt atgccataac
    12841 ggccagctaa acgagaagcg agcaccaccc agttcgctga tgtcacaatt aaccgtaccg
    12901 cccattgcca gtgctattga gtggacaatt gccagcccca gcccgcagcc gccggttgag
    12961 cgatcccggc tgggatcgag gcgaacaaaa ggttcaaaga tatgttcgcg gttttctggg
    13021 gcaatccctg ggccatcatc ctcaacaatt aatgtcgctc tattccccga cagtagcagg
    13081 ctggtttcaa ccgttgaatg gcagtagcgc agggcgttat tgagcaaatt atccagcacg
    13141 cgctccatta agcgcatatc caacgccgca taatggcctt gcatgagcgt ttttatccgt
    13201 accgttttat cgggcgttac tgcctgaata tctgccagat gcgttgacag ccacaacggc
    13261 aggtctggtt cgctaagatg aagctcgttt tgtgggcgat cgagtcgggc ataagtcagc
    13321 agctcttcaa ttaaagcttc aagttgactg atatcacgat tcaacgcctg ggattcggcg
    13381 gcgctcaggt tatcgctcat ctccagtcga taacgcaggc gcactaacgg tgttcgcagt
    13441 tcgtgagcga taccgtcaat aagctgtttt ttgctggcaa ttaaggcgtt gatattgtcc
    13501 gccatctggt taaatgcgat accaagtcgt tcaaagctcg aaccctcatc aaagtggata
    13561 cgttcattga gatgcccatc gccaaatcgt tgcgccgctg cttccagttt taacatatcc
    13621 tgccagtgcg gacgcatcca gataaacacc ggaaaggcga gggaaatagc aataaaagcg
    13681 atcagggcga tatccagcaa tcgcatttga tggaggtaat aaagataagg aacaggacta
    13741 actgccagca cgtagtggct gcgcgggata cgctgcaaaa acgtgtactg atcgtccagg
    13801 gcgacaattt cgccgccacg cagtcggtgc atggaaatat catcaagatg gtatttactc
    13861 agtggctcga cacgcagatc gaaagagaga tttaaatcca tctctttcag cgtcttaccc
    13921 cagtcgtgtg gggggatctc tcgcaattcg ctgcgcatca gatacagcga actgttcatc
    13981 aaatcatcca gcgactgttt gcccgcgcgt tcggcggtaa atttgtacac cagcccaacc
    14041 agcagagaca tcacaaggaa gcagacaaac aataacaggt aaaactggat aaacaatttt
    14101 ttcattggca tcgcccgcaa cagaatacta ttcgtattaa ttcacgactg tggagcaatg
    14161 taaaccgcca actggataag accttgtttg gaagataatg tccggaacag acggatgtat
    14221 ccccggtgta ttcatattca gtaaattcaa ttttccaccg gggcttgttt attatgctaa
    14281 ggtttaattt aacgtgacgc tttgttgttc tttagtctct ctatccttaa gaagacgggc
    14341 tgatattaca ccggacatta ttgaaccatt cacattaagc gctgttcttg ccatatcaac
    14401 gatcggctct atggcaataa aaataactac taatgcgaca gggaaattaa gtgttgaaag
    14461 gacaatcagc gccgcgaatg tcccaccact acctacgcct gctaccccta ttgagccaag
    14521 cgcgatggca ggtaaaagag ttgcgaggaa ttgcaatgag aagggatcga tacccattgt
    14581 tggagcaatc atcgccacca tcacggcagg ataaatccca gcgcagccat tttgtcccat
    14641 gctggaacca aaagacgctg caatattagc aatcgtattt ggaaccccca atttttccaa
    14701 tgcagaaata gccagaggaa ttgaagccgc actactgcga gaaacaaacg caaaggtaag
    14761 tacttgccag acggtcttgt aatagttaac ggggttttta cctattaaca gcaaaatgcc
    14821 cgagtgaaca ataaacatca caaaaacggc cagataacag gcgacgatga acccgagcaa
    14881 attggtaaac tgtgaaagct gatacgttga gaagactttc gccattagtg ccattacccc
    14941 ataaggcgtc agcgcaatga caatacggac cattttcatt acccatattt gaatcgcgtt
    15001 gatccccgcg ctcaattttt cgccctctgc tggtgcatct ttctttactt ttaacagcgc
    15061 aataccagca agaattgtaa agatgacgat gccaataacc gaaacacttc ttgccccggt
    15121 cagatcaagg aagaggttgg ttgggatcaa tgacgtaatg agtcgcggta aagataccgc
    15181 agctgttttc gagacgtcat cgctggttaa agaggattgc atatgaccaa aggcgctggc
    15241 atcaaggcca attgcactgg caatagccag gccaactaaa cctgcaatca tgactaaaca
    15301 caacatacag gcaacaatag ttaaagacat cttgccaata ccagcgctat tttcgagctt
    15361 attaatcgct gaaagaatcg aaatgaatat gagtggaacg gcaacaagtt ttaataaatt
    15421 aacataaccg ttaccagcaa tattatacca ttcgataacg gtttcactgc ttgtctcagg
    15481 aatgaaatgc agggagatac caatgatagc ccccaggaaa atcgcgataa aaacactttt
    15541 ggaaagggat tcgctctttt gatccgtttt gaaaatcccc catgagcaga tcaaaaaaag
    15601 cacaatatat gtgaaagttg taaccgtttt ttaagcctct tgcctcattc agcacctgaa
    15661 atgccggaaa gagtttgttg taaaaaatct gcgccataag acgcagggac gaggcgatta
    15721 tagagaaatt ttttctcatt ttcatatcaa atgtaggggg tttggaattt gaataagctc
    15781 ttattaattg aattcgttaa ttgttctctt tttcagatga acaaatatta gtcatctgat
    15841 tgtttattgt cactatccca tgcatgaggc gcaaaaagat agcctttgtt acgcacagtt
    15901 ttaatgcgat aaggttctgc ggcgttatcg agcagttttt ttcttaaccg cgaaatagcc
    15961 acgtccacgc tacgatccag tccgtcataa ctgacgccgc gtaaattttt cagcaatgca
    16021 tcgcggtcca tgatttgccc ggcatgcgta gctagttccc acaataattc gaaatcagct
    16081 gtcgagagcg agatttcagt gttagccagg gtgactacgc ggttgatggg gtcgatagtt
    16141 aacgtgccga aatgcagggc tttgtaggga gtaagagacg tttcctgaag acctttggtc
    16201 agtgtggctt gctcattctg acgcaaatgc aaacgtaaac gcgctagcaa aacagcaggg
    16261 ggcgtcgttt tgagaatata gtcgcaggca cccatttcca gtgccaggat gtggttcata
    16321 tcgctatcga gagaggttag aagaacaatc ggtccagacc actttgcgcg taaatcacga
    16381 caaatggtca tgccgtcctt gcctggtagc atgatgtcga gtaacaccaa atccggattt
    16441 tctcgcaaaa tggtttcttc ggcctggtcg ccgcgcggtt ctacggtaac ctgcatatca
    16501 tgttttgcca ggtacgcggc aatcagtgaa ccgacttccg catcatcttc cacaaatacg
    16561 atagtgttca taacattcac ggtagatata aaaacgtcac aatacaccgc gctgttttta
    16621 cttactatca atcgtttcta aacaagtttt catcaggtat tctactgatg acttgttatg
    16681 gaagtgttaa ggtaaaaaga tggggctggt aatcaaagcc gcgctcggtg cgctggtagt
    16741 gctgttgatt ggtgttttag caaaaacgaa aaattattat atcgccgggc tgattccact
    16801 tttcccgacc tttgcgctta tcgcgcatta tattgttgcc agcaaacgcg gcattgaagc
    16861 cttacgcgca accatcattt ttagtatgtg gtcgattatt ccctattttg tttacctggt
    16921 gtcgctgtgg tatttcaccg gaatgatgcg cttgcccgcc gcttttgttg gttctgttgc
    16981 gtgttggggg ataagcgcat gggtgttgat tatatgctgg ataaagctgc attaacgcag
    17041 atgacgaccg ccatcaagtg ggaaactgcg tccggtgaca aagcaactgg taagtaagta
    17101 atcgaccagg tcgatcactt ctttctcgcc aggcgcggtt ttcatcagtg atttattcag
    17161 cgcctgttgt cgatattcgg catcatcatg ttcattaaac aggatcagcg atggcgcaat
    17221 agaattcact ttcacttccg gtgccagctt gcgggcaaac gagcgggtca tattatccag
    17281 tgccgctttg cttgcagcat acgcaacatg tttgtcgcta ccgcgctcca ccacataatc
    17341 ggtaaagtga atgatatcgc tggcggcgtg tccgtgccca cgcagtaatc tttccagcgc
    17401 atggttgagc aggtatgggg tattaacgtg gatctgcatc atgcaagcca gtacgtcggc
    17461 cagtggcgca cccggttttt ccgcaatcca cgcactggcg ttatgcaaaa tagcacgcag
    17521 accatgggtg gtttttagta cttcatcggc aaacgccatc acaccgtcgt tggtcgaaaa
    17581 atcagcctga atacactgcg cacctgcatt aatcagtcca tcaatggctg gatagtgtgt
    17641 ccgatagctg acaatcaccg gttgcttttg attaatgaaa tgccatgcga gggcgaggcc
    17701 gatgcgacga cctccgccag taattaatat tggcaagggc tgggttttac ccatcgttat
    17761 ctcctttgct atccaacgat gggatgaacg ttatcccacc agcatccagg tcgccggaac
    17821 tgaggcaatt aacagcatgc caatcagcac catttcctga cggttcagta cattatcatg
    17881 tgtatgcgtt ttgcgcgcat acaggaaaac cagcagcccc ggtgcataca gaacaacgga
    17941 taacagcaag tgcattggac cagaggcgta caataaccat aagccataaa tgcaggcacc
    18001 gacacctacc gctttatgca gtggacgggt tgcgattttc agcaagaacg cgccgaccag
    18061 gaaatagggc accagaatca tttctgaggc gatggtcagc aacgtgttgt aatcggaacc
    18121 ggtcagccag atgagtacca gacaaatttg cacgcagatg ttggtcaacc acagtgaggc
    18181 ggatggcgca gcttgtgcgt tctggcgcgc aaaaatgcgt gggaatgctt tatgagtggc
    18241 agccaggaac ggaacttctg ccgccatgat ggtccagctt aagtacgcgc cgcaaacgga
    18301 aacgatcaaa ccggcagcga tgatgatttc gccccatggt cccatcattt ccaccatcag
    18361 accggccatt gacgggttac gaatttcagc cagttcagga cgtgccacca cacccagcga
    18421 aagcagcgtt accagcaagt aaacgcccag agcggagaga accgccagca gtgtcgcttt
    18481 gccaacatca cgtttattac gcgcacgcgc agaaacaacc acggcacctt ccacaccaat
    18541 gaaaacccac agggtgatca gcatggtgtt tttcacctgt tcccaaacag gtacgccaag
    18601 tgcaagtccg gtgaagtcga gcttgaaggt atccagtttg aacatcatca tcgccagcac
    18661 aacaaacaga cccagcggca acaattttgc cagtgtcgcc accaggttaa tgctggcagc
    18721 ggtttgcacc ccgcgcagga tcaaaaagtg aacaatccac aataaagccg atgcaccgac
    18781 tatcgattgc caggtattac catcgccaaa caggcgcaat tccggcgtgt ccgtaaaaaa
    18841 gcttaacgcg gaaaaaacga tcaccagata ggagacgttg gcgatgaccg cgcacagcca
    18901 gtatccccat gcggaacaaa agccgattag ctcgccaaac ccttcgcggg cataggtaaa
    18961 gataccgcca tcaagttcgg gacgaatgcg cgtgaggatc agcatggcaa aggccagcaa
    19021 taaaatgcca gcgccagtaa taccccagcc gatgagcagt gctgccgggc tggcaactgc
    19081 cgccatattt tgcggcagac tgaaaacacc cgcgcccagc attgagctta ataccagcgc
    19141 ggtgagtgcg ctcagtccca gtttcttttc catcgatatc cagtgattaa aacgtgataa
    19201 gaagaataaa tattagggca aaacgcataa aaatggagtt tgccactgaa gcgcgggatt
    19261 ttacgaagag atgtgtctgc atgcaatggt cgcggggaaa tttgtgacta aaaatgcgaa
    19321 aggcggcatc aagccgcctt ataggagtaa ctgaatagtt atttatacag atctgcgctg
    19381 acggtcaggt tattaccacg tttctgccac tggcgggtga tgtggtaata cttagcacct
    19441 ttcttcgcgg cacgtttcgc aacctgatag gagacttcgg tcatgttgcc gtagttgcca
    19501 gagaatttga tgctgtcgaa cgggaccatc atcgctgctg tcgctttgtt cagttcttcg
    19561 actttagtgc catccgggag cgtgacggtg taacgcccgc cttttgatga ctgggtttca
    19621 aagaagcgac cgacttcaga acttggtgat gcggtagtcg caacacccgg gatctcaact
    19681 ttcttcgcgg cttcgccacc ggcagccaga gctgcacgtc ctgcttcgga atctgccggg
    19741 atcacatccg ggctctggac gatacgtttc ttagcatctt ttttatagat gaatgcagta
    19801 atacgctggt tgccgccctg gttggcatcg atttgacgaa cgatgtagaa agagtaggca
    19861 cctttcgctt ttgccgcttt ggtgatggca tcattgactt ctggctggct acgatagaag
    19921 ccctggacgg tgaccgtgtc aaacggttca atcagaacag cctgatcttt cggcagttcg
    19981 acaacaccgt taattacgcg attacttgtt tcttctgctt tttcagcatc ggctttatag
    20041 aggtcagcga ccacacgcca gttaccgctg ttaccaaaat cagaagtgtc gacaacataa
    20101 aaagaggcgg caccttcttt atctgcgcga cgagaaacgg ctttcaccgc ttcgccaata
    20161 gcattaaaac gaccggtaac cactacacgg tcaaaaggtt taaccgctgc cgcttgctcc
    20221 ggtgtcagtt ctgttgctgc gttaacggag aatgccgtag cagaaagcag tgccgacgcc
    20281 aggagggtgt tcttaagctt cataaaaata atccttcgcc ttgcgcaaac caggtactgg
    20341 tattgttatt aacgagaaac gtggctgatt attgcattta aacggtgtaa ctgtctgcgt
    20401 catttttcat atcacattcc ttaaaccaat tttaatcctg ctcaaatgac cgtctatgct
    20461 taaaaaacag ccgtatcagc atcattacta ctgaagcaac tgaattgtat aagttaattt
    20521 aatgttaagt agtgattcgt gccggggcga tgtctcgttt tacccgaccg tcgaagacaa
    20581 ttatcagtct ttatccggcg ttctaaggtg tttatcccac tatcacggct gaatcgttaa
    20641 tattttgcga gttcacgccg aaatactgat ttttggcgct agatcacagg cataattttc
    20701 agtacgttat agggcgtttg ttactaattt attttaacgg agtaacattt agctcgtaca
    20761 tgagcagctt gtgtggctcc tgacacaggc aaaccatcat caataaaacc gatggaaggg
    20821 aatatcatgc gaattggcat accaagagaa cggttaacca atgaaacccg tgttgcagca
    20881 acgccaaaaa cagtggaaca gctgctgaaa ctgggtttta ccgtcgcggt agagagcggc
    20941 gcgggtcaac tggcaagttt tgacgataaa gcgtttgtgc aagcgggcgc tgaaattgta
    21001 gaagggaata gcgtctggca gtcagagatc attctgaagg tcaatgcgcc gttagatgat
    21061 gaaattgcgt tactgaatcc cgggacaacg ctggtgagtt ttatctggcc tgcgcagaat
    21121 ccggaattaa tgcaaaaact tgcggaacgt aacgtgaccg tgatggcgat ggactctgtg
    21181 ccgcgtatct cacgcgcaca atcgctggac gcactaagct cgatggcgaa tatcgccggt
    21241 tatcgcgcca ttgttgaagc ggcacatgaa tttgggcgct tctttaccgg gcaaatcact
    21301 gcggccggga aagtgccacc ggcaaaagtg atggtgattg gtgccggtgt tgcaggtttg
    21361 gccgccattg gcgcagcaaa cagtctcggc gcgattgtgc gtgcattcga cacccgcccg
    21421 gaagtgaaag aacaagttca aagtatgggc gcggaattcc tcgagctgga ttttaaagag
    21481 gaagcgggca gcggcgatgg ctatgccaaa gtgatgtcgg acgcgttcat caaagcggaa
    21541 atggaactct ttgccgccca ggcaaaagag gtcgatatca ttgtcaccac cgcgcttatt
    21601 ccaggcaaac cagcgccgaa gctaattacc cgtgaaatgg ttgactccat gaaggcgggc
    21661 agtgtgattg tcgacctggc agcccaaaac ggtggcaact gtgaatacat cgtgccgggt
    21721 gaaatcttca gtacggaaaa tggtgtcaaa gtgattggtt ataccgatct tccgggccgt
    21781 ctgccgacgc aatcctcaca gctttacggt actaacctcg ttaatctgct gaaactgttg
    21841 tgcaaagaga aagacggcaa tatcactgtt gattttgatg atgtggtgat tcgcggcgtg
    21901 accgtgatcc gtgcgggcga aattacctgg ccggcaccgc cgattcaggt atcagctcag
    21961 ccgcaggcgg cacaaaaagc ggcaccggaa gtgaaaactg aggaaaaatg tgcctgctca
    22021 ccgtggcgta aatacgcgtt gatggcgctg gcaatcatcc tgttcggttg gctgtcaagc
    22081 gttgcgccga aagagtttct tgggcacttc accgttttcg cgctggcctg cgttgtcggt
    22141 tattacgtgg tgtggaatgt atcgcacgcg ctgcatacac cgttgatgtc ggttaccaac
    22201 gcgatttcag ggattattgt tgtcggagca ctgttgcaga ttggccaggg aggctgggtt
    22261 agcttcctta gttttatcgc ggtgcttata gccagcatta atattttcgg tggcttcacc
    22321 gtgactcagc gcatgctgaa aatgttccgc aaaaattaag gggtaacata tgtctggagg
    22381 attagttaca gctgcataca ttgttgccgc gatcctgttt atcttcagtc tggccggtct
    22441 ttcgaaacat gaaacgtctc gccagggtaa caacttcggt atcgccggga tggcgattgc
    22501 gttaatcgca accatttttg gaccggatac gggtaatgtt ggctggatct tgctggcgat
    22561 ggtcattggt ggggcaattg gtatccggct ggcgaagaaa gttgaaatga ccgaaatgcc
    22621 agaactggtg gcgatcctgc acagcttcgt gggtctggcg gcagtgctgg ttggctttaa
    22681 cagttatctg catcatgacg cgggaatggc accgattctg gtcaatattc acctgacgga
    22741 agtgttcctc ggtatcttca tcggggcggt aacgttcacg ggctcggtgg tggcgttcgg
    22801 caaactgtgt ggcaagattt cgtctaaacc gttgatgctg ccaaaccgtc acaaaatgaa
    22861 cctggcggct ctggtcgttt ccttcttgct gctgattgta tttgttcgca cggacagcgt
    22921 cggcctgcaa gtgctggcat tgctgataat gaccgccatt gcgctggtat tcggctggca
    22981 tttagtcgcc tccatcggtg gtgcagatat gccggtggtg gtgtcgatgc tgaactcgta
    23041 ctccggctgg gcggctgcgg ctgcgggctt tatgctcagc aacgacctgc tgattgtgac
    23101 cggtgcgctg gtcggttctt cgggggctat cctttcttac attatgtgta aggcgatgaa
    23161 ccgttccttt atcagcgtta ttgcgggtgg tttcggcacc gacggctctt ctactggcga
    23221 tgatcaggaa gtgggtgagc accgcgaaat caccgcagaa gagacagcgg aactgctgaa
    23281 aaactcccat tcagtgatca ttactccggg gtacggcatg gcagtcgcgc aggcgcaata
    23341 tcctgtcgct gaaattaccg agaaactccg cgctcgtggt attaatgtgc gtttcggtat
    23401 ccacccggtt gcggggcgtt tgcctggaca tatgaacgta ttgctggctg aagcaaaagt
    23461 accgtatgac atcgtgctgg aaatggacga gatcaacgat gactttgctg ataccgatac
    23521 cgtactggtg attggtgcta acgatacggt taacccggcg gcgcaggatg atccgaagag
    23581 tccgattgct ggtatgcctg tgctggaagt gtggaaagcg cagaacgtga ttgtctttaa
    23641 acgttcgatg aacactggct atgctggtgt gcaaaacccg ctgttcttca aggaaaacac
    23701 ccacatgctg tttggtgacg ccaaagccag cgtggatgca atcctgaaag ctctgtaacc
    23761 ctgacggcct ctgcggaggc cgtcactctt tattgagatc gcttaacaga acggcgatgc
    23821 tttgacctcc cgctgtttgt tcaagcgcaa ttttgacaat aattgtcaac ggcacggaaa
    23881 gcagcatacc caccggtcct aacaaccatc cccaaaaaat caacgacaaa aataccacca
    23941 atgtcgaaag ccccagccca cgtcccatga tgcgcggctc cagaatattg ccgaagacca
    24001 gattaatcag cagatatccc gccagcacca gcaacgcttc gtagaagcca ttaaacacca
    24061 gtacctgagc gatagggggg attgccgcga gaactgaacc aatattaggg atgtaattaa
    24121 gcgcaaaggc cagcaatccc cagacaaaag cgaagcgaac atcgagtgcg gcgagcatcg
    24181 cccaggcgac caggccggtg atgatgctga tggctgtttt cagcaccaga taatgagaaa
    24241 cactgtcaat cgcacgttga atcgccgcca tcccttcaac cggacgcgcc atcatttgct
    24301 gaaattttcc gggcaattgt ggcacttcga gcagcataaa cagcaccgtc agcagcaata
    24361 aaaatattga tgacatggca ttagataact gcgtcaataa gttggtgagc aacgtcatcg
    24421 ccgcgttcgg atcaatgtaa tgcgccagtt ggtcaactga gacgtcaatc cctacgcgtt
    24481 gcaacaacgg ctcaagagct tgcagcggcg tcataataga gttgcgatat tgcggtaacg
    24541 tccacgtcaa ctcgttgagc gcggaaccca gataagctaa tagcaacacc atcgccatca
    24601 cgatgattgt catcaaaatc gacactgcca gtacacgtgg cacacgccag cggaccatat
    24661 gttgcaccag cgggttaaga ataacagcaa taaataatgc gagaataaac ggcacgatga
    24721 tctcggcggc aaaacggata ccgcagagaa taatgaccag cattcccaac ataatgacga
    24781 tttttaggcc attgagcgtg atgatcggct ttgccatgct tgtccccgtt tttcttcctt
    24841 tgctaataat aatgtctttt tcgctccgtc atcataacga aaatcaaacc gctcaggtaa
    24901 agaagtgaaa atattttgag ttaattctta agctatgata caaatcaggc gtgttcaact
    24961 accgaggaca attatcatct gcgatgacga gaagcaacac tgcggataat tgtaatatta
    25021 tggacaattt gttcaggacg ttattttcta catttaccca tcttcgtacc tcatccacta
    25081 tcttgctcgt aggtgagcag cactggcgca acgcgcttta gtcctttctt ctgcctaagc
    25141 ttgccctatg cgcagggctt gaccacttta ctttggtttc gctgaattaa gcgaaaatta
    25201 aaatagttct cttgcaggag aaggacaatg tatatttatt ggattttatt aggtctggct
    25261 attgctacag aaattaccgg tacgctgtca atgaaatggg cgagcgtcag tgagggaaat
    25321 ggcggcttta ttttaatgct ggtgatgatt tctctgtcgt atatatttct ctctttcgcc
    25381 gttaaaaaaa tcgccttagg cgtagcttat gcgctgtggg aaggtatcgg tattttattt
    25441 attaccttgt ttagcgtttt gttattcgac gaaagtttat cgctgatgaa aattgccggg
    25501 ttaaccaccc tggtcgccgg gatagtgttg atcaaatcag gtacccgtaa agcgcgtaaa
    25561 cctgaactgg aggtgaacca tggcgcagtt tgaatgggtt cacgccgcct ggctggcatt
    25621 ggcaatcgtg ctggaaatcg ttgctaacgt ctttttgaaa ttttctgacg gctttcgtcg
    25681 caaaatattt ggcttgctct ccctggcggc ggtgctggcg gcctttagtg cgctttctca
    25741 ggccgttaaa gggatcgact tgtctgtcgt ttatgcattg tggggcgggt ttggtattgc
    25801 cgccacgtta gccgcaggtt ggatcttgtt tggtcaacgg ttaaatcgta aaggctggat
    25861 tggcctggtc ttgctgttgg ctggaatgat catggtgaaa cttgcctgat gaagacgctg
    25921 cccgcgctgt cgggcagcgt ttgaacatta tttttgcgac agttgatcca gcttgtcgcg
    25981 aaaaccggta acagaaatgg cccggttatc ggcgcgccag cgatctttcg cggcaggagc
    26041 cgaactttgc accccaatta attgccagcc gtcatcggta tgcaacatca gaggcgaacc
    26101 gctgtcaccc ggcaaggtat cgcactgatg tgacatcacc gacgtttgcg cccagccagt
    26161 cacttcacag ttttgatgac tgtataacgt atcgagatga tcttcagggt atcccgcctg
    26221 agtcacttta cgaccagccg attttaatgc ggcagtaagc gcggctttat ctccctcaaa
    26281 taacggcaac ggtgtaatgc cagaaggggg attacgtagc acaatcaatc cgaagtccca
    26341 cggtgcagct gcgggaggaa caatccaacc atccccatct gcttttaacc gctttcccag
    26401 tgtcggatca acgcggcctt ctatgtcgtg gatctcatag cgccaaagac ctttatttga
    26461 cacaaaacgc agcgctaccg ccttatccgc tttaccctta ggcggcgtca ataaacagtg
    26521 tcctgccgtt aatgccagat tgggtgcaat cagcgtcgcc gtacataaat tgccgctggc
    26581 cgtttccagt tgcccaaccg catcccacgg tgattgggtc gtgtcattca ctggcacacg
    26641 atcatcatga ccaaaaaaca gggtgctgac ctcatcgttt gccgatttgg caacgtctgg
    26701 tttatctgca aacacaaaag cagacgtcaa actaattgca cccaacacta cagcaatggt
    26761 tgtacgcata tcacactctg gtgggtaatt atgattatta aaagcaaacc ctcataaata
    26821 ctatagacgg gacgggatga aagtgggagt aaaatcagat aactacagtc aggaaagata
    26881 aaaacgggcg gcaatcagtg cgcataaaat aaggatgata agaatgaact caaagcgata
    26941 acggcccacc atactaccct ccgctaaagg cggtgctaaa cgttacccgc ttagcaccgt
    27001 aattttcagg cgcgaggggg cgcgccagca ttactgttga aaacttacgc tgcgggttgt
    27061 gcagcaggtt ttgccggttg ctgatggccg tgtttcccgg cgtgtttctt cgctgcctgc
    27121 gctttttgtt caggggcttt ctgttcagct ttcgtatttt tatgatgctt tttagccgcc
    27181 tgcgctttct gggcaggtgc tgctttatgc tgttttttat gatgtgtagt tttcgctggc
    27241 gctgctttgg tggtcgtcgc agtcggagca ggtgtggtcg cagtctctgc agcaaaggcg
    27301 gcagaagaca gacccatagc agcggcaaca accagagcta atactttttt cattgtcata
    27361 ccctcaattt gttttttcat ttaaccccac tgcggggccg ttgaaataac tatatccctg
    27421 ggaatggcag acttccgtga gtggttggtt tcagcgtgta acgatatgta caaacgctga
    27481 ataaattaca gcgttgataa taacgtattg tgacttaagg aaaatttagc taccaataat
    27541 agtagtcttg atcgggttaa ctattatact acttacaaag cggtagaata actgcgcatc
    27601 agtaataaat gacacacagc aaaatgaatc cgtttatttg ggtacttata atcctgatga
    27661 cgctagatgc gctgcgggaa ttggctggcg cttcgtctat tttaggatgg ctattaacgt
    27721 tggtttgagc aggcaataag tccggacggg tatttaccgc agtccggact tatttttcag
    27781 gcgtgcagac gacgatgcaa acgcgtcccg accagcagag caatgaccag catcagagca
    27841 ataaatgcgc cgacgccgtt ccagccatag ttatgccaga aaacaccacc cagcgtcccg
    27901 gcaatactcg accccagata gtaactgaac agatacagcg aggaggcctg gcctttagcg
    27961 cgttttgcgc gggggccgat ccagctgctg gctactgagt gggctgcgaa gaatccggct
    28021 gagaagagta acattccggc aaagatcagc cacagcgagc taaataaggt catcagtaaa
    28081 ccaaacggca taactcccgt cgaaaacaac atcaccggac cgcgcccata gcgggtggtc
    28141 atggttccgg ctttgggtga gctccatgta ccggtcaaat aagccagcga taataagcca
    28201 accacggcct gactgacatg ctagggggaa agcatcaacc gatagccgat gtaattaaac
    28261 agcgtgacga acgaccccat cagcaaaaag ccttctgcga acaataacgg taatccccgg
    28321 tcacgccagt gcagacgaaa gttgataaat aacgtcttag ggcgcagcga agtcgggcaa
    28381 aaatggcgtg attcagggag gattttccag aacatcaacg ccgaggccag cgcgaaacaa
    28441 ccgattgccg ccagagcaat tcgccagttg aaaaagtccg tgaagacacc actaattaag
    28501 cgtccgctca tgccgccaat tgagttgccg ctgatataca accccatcga aaaggccacg
    28561 aaactggggt ggatttcctc gctaagataa gccatgccaa cagctgccac gccacttaac
    28621 gaaagcccaa tcaaggcgcg cataatcaaa atgccgtgcc agctggtcat cattgtcgaa
    28681 agtaacgtac aaatggaggc caacagtagc gccgtgacca tcactggttt gcgaccaatg
    28741 gcatcggata gcgggccagt aaatagcaaa ccgatagcca acatcgccgt ggaaatggac
    28801 agtgaaatac tactgttcgc gggcgttaag ccaaactcct gcgaaagcac cggaaggata
    28861 ggctgcacac aatagagaag tgcaaaggtt gccagtccgg cagagaacag cgccagggtg
    28921 acgcgcataa attgcggcgt accgcgttta ataaattgat ttggctgaga aatgctttgc
    28981 ttgtcagtgt cgcttgccgg agcgccatca acagttgtag tacggctcac ttgaaatcct
    29041 tgctaaatat gcctgtggat caggcttata catagggtag gaaaatcgaa ttgttctgtc
    29101 taatatatta ataatctcaa ataagatgtt ttaaatatga atattgaact tcgtcatctg
    29161 cgttactttg ttgctgttgc ggaagagctg cattttgggc gcgcggctgc ccgcctgaat
    29221 atttcgcaac cgccgctaag tcagcagatt caggcactgg agcaacaaat cggtgcccga
    29281 ctgctggcac gaactaatcg cagtgtattg ctgacggcag caggaaaaca gtttcttgca
    29341 gatagtcggc aaatcctgtc tatggtggat gacgctgccg ctcgcgctga aagactgcat
    29401 cagggtgaag cgggggagtt gcgtattggt tttacttcgt ccgctccttt tattcgggcg
    29461 gtgtccgata cgttatcgct gtttcgccgt gattatcctg atgtccattt acaaacccgc
    29521 gaaatgaaca ctcgcgagca aatcgctccg ctcattgaag gaacgctgga tatgggattg
    29581 ctgcgtaaca cagcgttacc ggagacgctt gaacacgcag tcatcgtcca tgaaccgctt
    29641 atggcgatga tcccgtacga tcatcccctg gcaaataatc cgagtgtaac actggctgaa
    29701 ctggctgaac tggctgaact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg
    29761 cacgtcggga ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg
    29821 cccgtcatca ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt
    29881 ctgggtgttt caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg
    29941 gtgccgattg ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat
    30001 gaacaaagtc cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg
    30061 aaatttcagc gaaaaagccc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg
    30121 acgacacgta ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata
    30181 gggagtatgc ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca
    30241 acgcgggcgc ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt
    30301 cccgtctggc gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag
    30361 cacacctggt gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg
    30421 ggctggtggt tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga
    30481 ttttccttgc tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg
    30541 cgttaaaaga tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta
    30601 tccgccacca gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta
    30661 ttgatacgga aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc
    30721 cgctcggcga ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca
    30781 gcgcatggac gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc
    30841 aggtggttat cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg
    30901 caggcagcag tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct
    30961 gttattgcgg gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc
    31021 tggcacagct gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg
    31081 tggactcatt gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg
    31141 gggtgggcgc gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa
    31201 aaatactgat tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag
    31261 acagcatccg tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc
    31321 agttttctaa ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg
    31381 gttctttgtt gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg
    31441 cgctatctca atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct
    31501 agtttatatt tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc
    31561 ggtacagaca cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc
    31621 tcccagggaa aaagggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc
    31681 gaagggctgc gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct
    31741 tatgaagcag ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc
    31801 ccaatcaatt acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat
    31861 gtcgtggtag aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct
    31921 gaatgggtag tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc
    31981 attaaccatg cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc
    32041 tgggtggcta accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg
    32101 ggtaaaaaac ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag
    32161 cgtgaactgg ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga
    32221 gtcacggtgt aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg
    32281 gagtagctga tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg
    32341 cgtggttgcc gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc
    32401 gccatcaggg aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat
    32461 aaatagcgtc ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc
    32521 acacagttta caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa
    32581 ggattgtacg gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc
    32641 cacgatcaac ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc
    32701 acgatgacag gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc
    32761 tgtactgata atcagcgcat agtcacgagc ctgaaccgtc actgagagtt gtacgttgta
    32821 gagtaacgcg tcgctatgat taatcagatt gctaaccagc catgccacga tggcggaaat
    32881 aatcaccggg ccgagagagg ccaacatcat agtgccaaac agcacttcgg ctataaataa
    32941 actgccagca agcggggcac gataggccgc agccattccc gccgcggccc cacaggcgat
    33001 ccataatttc cattcctggc gtggcgtaaa acgttgggca aaacaggagg cggcaagggc
    33061 agctaaaaga atcatcgcac cttcgcgacc aattgcactg ccgctggtta ctaccagcag
    33121 cgaggcaagc gatttaacca ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc
    33181 ttccatgtaa tcggtcggcg catgagggcg ttgttgggta aatttctgcc agcccatcag
    33241 caacaaaccc gccgccagtc cgccgagcgc cggagttagc agccgtcgcc aggaggaaag
    33301 gtttgtcgct gcattgacca gactgccgga gtcattattg aggaacaacc actccagtag
    33361 cagcatcgca tgacgaaacc cggcaacggc aaagacagcg agaataccga cgactgttgc
    33421 gataagcaga cggcggaaca tagtgcgcag atccggatag atgtgtagat gatgcatgag
    33481 cagaaatggg caatgaaaca cgggcgacta ttttgcggca aaagaggcca accgcaaaag
    33541 cagtgcgtgc tttaagagat aaaaaaacgt gacattgtaa cgtattattg ccgaatcgcg
    33601 cgtaatcgtc accatccggc aatattacgg tgatcctatc gaaagagtgg tttaaccgcg
    33661 acaggaatca acagttgtga ttgccattgc gccagcgtta agcgagccag ttcacccagt
    33721 gcacggtaga aagggtgttc tgctttttcg ataaaaacat caagaaaacg tgttgaccac
    33781 ggaaaaaggt gccatgccag caattcttcg cattctgtct ggcgaccatt ctccgccaac
    33841 cacgcagcca tcaacagcag tgaaccaaaa tgatcttccg gttcgttttg cttcatttca
    33901 aactgaatgc ctttctcgcg catccactga cgaagtgcca atgttgaatc gccaaacagc
    33961 acagattcgc gatccagcca gaccgacccc cacggcggag acggcagtgc ccacgggccg
    34021 acaaacaaac gctgccaggc ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc
    34081 accagcggcg ctaatgacgc ttctggtaga ggccactgag tttcccagcc gtcactggtg
    34141 agtaccgcaa ccagaggtgc ggcttccgca ctctctggag cgtaataaaa cagcgcgccc
    34201 aacacgcgcg ccgcgacaga aaaattatct tgctgtgaaa aatgggtcat tcctaacatc
    34261 ctgaaatgtg cgggttgccc cgcaccaatt tgttaacctg caatagccat tcctactgtc
    34321 atgtgcaggc cataaaagag cacacgcccg atcatctcac caacaagtat gaggattagc
    34381 cccagaacaa gtccggcaac gtgaggttca cggcgacgaa tgagcggaca aagccagcat
    34441 ccaagacctg cgcataacaa taccacacgc cagacctgta aggaggcgta atctggtacc
    34501 agtgcgctgg cttgttgcac ggatgagtga atagacgcca gagacaaacc ttgcagcaca
    34561 atcacctcgg cacaagcgat cagcgccaga acgctaatga tggcaaatgg tgtggtatta
    34621 aatgtaacgc gggctgcccg cagaattgcg gcagcaagta ttggaccgct caacaatacc
    34681 gtcaggaaga aagccagcgt agtgtaaccg ttatgccagg ttggcacggt gtcgatttga
    34741 tacacgcagg tcatcatcca gacgaaaatg acgccgagcg ccatgctgac aagtaaccag
    34801 agtttcccca acgcttgcgg cattttaccg atgaccgcca ccagccacca tagtccgcca
    34861 acggcaaaaa atatggaacc ggcagcaatc tcgttactca gaccagaagc tccgattcga
    34921 ttcagcgagt taaacgctcg cagtggcgat cccaggtgca tgacagaggc aataaacccg
    34981 atgcccatca acagccagag gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca
    35041 tttttggcgg cgaaccagcc aattccgcta acaatcagcg cgcctactac gcattgaccc
    35101 aatacggtaa agataaccag tggccattca tgccatccat ttcccatttt acacttcctc
    35161 cggattagcc agataaccag tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat
    35221 aacgatattg ggttttgtga aatgcgcgcg cggcagtggc gcgacggcag ccagagtgcc
    35281 gtgcttctgg cgcagctctt caattggacc gaactcaagt gcacgcagcg ggcaggattc
    35341 gacgcatatg ggttgtttcc cctcggcgac gcgcgaataa cagccatcgc acttcgtcat
    35401 gtgccctttt tcagcattgt actgtggtgc gccgtacgga caagccatgt ggcagtagcg
    35461 gcagccgata caaacatctt catcaaccac cacaaagcca tcttcacgct tatgcattgc
    35521 gccgctgggg cagacttttg tacatgcggg gtcatcgcaa tggttacagg agatggagag
    35581 ataataagcg aacacgttct ggtgccagat gccattatct tcctgccagt cgccgccagc
    35641 atattcataa atacggcgga aactgacttc cgggccaaga tctttaaaat ctttgcacgc
    35701 cagttcgcag gttttacagc cagtacagcg gctggaatcg ataaaaaatc catattgtgt
    35761 ggtcatgggc tactccttaa accttttcga tctggacaag attgctgtgc gacgggtttc
    35821 cctttgccag cggtgaaggg cggtgagagg tcagaatatt gatactgccg ccgtgatcga
    35881 cccggtcacc aaacatatcc gctttaagcc acgcaccttg cccgatggcg gtaacgccag
    35941 gcagaatacg cggagtcact tttgcggcaa tcagcatttc tccattattg ttaaataccc
    36001 gcacggtatc gccatgacgg ataccgcgtg cctgagcatc aatggggttg atccacacct
    36061 cttgtgggca ggcctgctgt aacacatcaa tattgccgta gctggagtgg gtacgcgctt
    36121 tggtttggcg acttcactca gcatccagta gatgggtttg cgttcaaatt ttgctgaggt
    36181 tgcgggttgg gcgaggatca catagcccat attccctgca gattcatgag agataaggtc
    36241 ttcttgctct gttggcatca ggtcaggcaa caggatatcg caatacttag ccgaggccgt
    36301 catgaagtgg tcaatgccaa caatcatctc gcacttgctg tcatcctgaa gcacctcatg
    36361 ggtgtgattg atgtcgccat gttgattgat caatgtgtta ctggcgtagc accataaaaa
    36421 cttgatgggg acatccagtt tttcttttcc acgaacacca tcacgggtcg cggtcatttt
    36481 cgtaccatgg tcgatggcat ctgtccatgt aaaaacggaa atctgcgttt taacaggatt
    36541 ctcgagcatc gggaaccatt ctacccccag atcccagcta ccttcgcgta cacctgagtt
    36601 gccgccgttt atgccgacgt taccggtaag aacggaaagc atggcaatag cgcgggacgt
    36661 ttgttcgccg ttggagtgtc gttgtggccc ccaaccctga caaatataag caggttttgc
    36721 tgaaccgatc tctcgtgcca actggataat tttttctgcc gggatgctgg tgatttttgc
    36781 tgcccattcc ggcgttttag ctatgccgtc aggcccttcg cccagaatat aggctttata
    36841 atgcgcgtta cgtggtgcgt tggcgggcag cgttttttca tcgtaaccaa cacaatattt
    36901 gtcgagaaat ggctgatcga ccatgttttc agtaatcagt acccaggcaa tcgcacaggc
    36961 cagtgcgcca tcggtgccag ggcgaatggg cagccattca tcttcacgcc cggcagctgt
    37021 gtcgttatag cgtggatcga tgacgatcat gcgtgcgttt gaacgttcgc gggcttgctc
    37081 gacgtagtaa gtgacaccac cgccgctcat ccgcgtttct gccgggttat ttccgaacat
    37141 aacgaccagt ttcgtattgg cgatatcatc cgggctgttg ccatcattgg caccgaacat
    37201 ataactcatt gcggcactga tctgtgcggt actgtagctg ccatagcgac tgagaaaacc
    37261 cccgcaagag ttcatcagac ggtacgggac gttcgagttg gtgatatttc cgccatctac
    37321 gcctgttccg tacaggacat gtacagcctc attgccgtaa tctttcagga tccgccgaag
    37381 attatcactg atggtatcca gggcttcgtc ccaacttatc cgttcaaatt taccttcacc
    37441 gcgctcgccg acgcgcttca tgggatattt caacctgtca ggatgattca tccgtcggcg
    37501 gatagagcgc ccgcgtaaac atgcccgaac ctgatgatta ccgtaaacgt cgtcacctgt
    37561 cgtatcagac tccacccagt acacggtgtc atctttcaca tgcaaacgta gcagacagcg
    37621 gctcccgcag ttaacggtgc aggaactcca gaccgttttc tcttctaccg gagcctctgc
    37681 cgcccggacc atttgggaaa atggcagagt gaaagcactg cttgccagcg cgagactgcc
    37741 aagtgcggag gttttcatca gacttctatg gctgatttca gccttcatga gcgcctctgt
    37801 ggtatggatt ttcatcatta ctcacttatt gcttttcaaa caaaatgtca tgccagaatt
    37861 tatggttgta gtgggttata ttttttcgat ctcgaccaga ttagtgtgct gcgggtttcc
    37921 cttcgccagt ggtgaagggc gcagagtggt tagcgtattc acacatccgc catggtcgat
    37981 tttatcgcca gacatattgg gctcgtgcca ggctccctgg cccatagcgc taactccagg
    38041 gagaatgcgt ggtgttactt tggctggtag ccgaacttcg ccacgatggt taaacacccg
    38101 caccatatcg ccgttggcaa tcccacgttt ctgtgcatct atagggttga tccacacctc
    38161 ctgacggcag gctgccttca ggacatcaat gttgccgtag gtcgagtgag tacgggattt
    38221 gtaatggaaa ccaaacagtt gcagtgggaa ggttctacgt tcaggggagt cccagccttc
    38281 aaaggttgag gcataaactg gcagtgggct tatcacttca tctttttcca gttcccaggt
    38341 acgggcaatt tccgccagct tgctggaata aatttcaatc ttaccggaag gcgttttaag
    38401 tggatttgcc tcggggtcgt cacgaaatgc tttgtaggcg acaaaatggc cattgggatc
    38461 tttacgctta tagataccca tttttttcag ttcgtcgtaa gacggtaacg ccggatcttt
    38521 ggcaagcatt ttggcgtaca gatgttgtaa ccattgttcc tgcgtgcgac cttctgtgaa
    38581 cttttgatag acgtcaggtc caagacgttt cgcgacttca ctcaggatcc agtaaatcgg
    38641 tttacgttcg aatttttcgc tggtgactgg ctggaggaaa atgagatatc ccatgttacc
    38701 ggcgtagtcg ttaggaataa tatcttcctg ctcaacggtc atcaggtctg ccagcagaat
    38761 gtcggcatat tttgccgatg aggtcataaa gttttcgatg accacaatca tttcgcattt
    38821 cgattcgtcc tgcagaattt catgcgtttt gttgatgtca gaatgctgat taacgagggt
    38881 atttcccgcg tagttccaga tggactccac tgtcagacgg atggacggac ttgaagtagt
    38941 tgactcaacg ctacttaagg tcctgatatg actgacatta accatgacga tactgcatat
    39001 ccatccagtt ctttctctgg gtctccctat ctgtatcagc aagtctacac ctcaagtcct
    39061 gacacaaggc tgacacaaat caaggacctt gctacgccac agctagtccg gagaaccaac
    39121 gggcgttaca ctgtccgctt cagacttaaa ggagctacca gcccgtttct atcagtctca
    39181 acccgcagca cagaccggag gaccgctaca atgcgtcaga gagagctcgc agccacagct
    39241 aaggcattcc tactggacaa tcctgaagtc acactacagg agcttacagc gcacatacgg
    39301 tccatggctg agcagttcct gactgatgcc agtgatgact actggaatgg cgtagaggtg
    39361 gcgacactgg ttgacgagaa gtccaacctc agggaacttg cagcgacaag agcgttgagt
    39421 gtggaccagc agaggggcat taagttagcg ctggaagtcc tgaccgcagc acagcagaga
    39481 gtcgacactg gtgatacctc tggtctgatt aagctgattg atggtggcgt aagccagctt
    39541 actgatgata ataaccatac tgattactca actataggcg actctacgtc gattctgaag
    39601 aatgagcagg gggatagacc agcagttttc acacaggaac gtcagttact cactccagag
    39661 tcacgagaat catctgtact ttttcaggaa ttggtgacat cactgctggc tgagaaggtc
    39721 cagacactga aacaatccag ctttaaggac ttgtcagcgt cc
//
LOCUS       DAOFMX010000033        38786 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.033, whole genome shotgun sequence.
ACCESSION   DAOFMX010000033 DAOFMX010000000
VERSION     DAOFMX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.033"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="QBA20_001904"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="QBA20_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HDQ6292456.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..571
                     /gene="hisL"
                     /locus_tag="QBA20_001905"
     CDS             530..571
                     /gene="hisL"
                     /locus_tag="QBA20_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDQ6292457.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="QBA20_001906"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="QBA20_001906"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDQ6292458.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="QBA20_001907"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="QBA20_001907"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDQ6292459.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAARERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="QBA20_001908"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="QBA20_001908"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDQ6292460.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="QBA20_001909"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="QBA20_001909"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDQ6292461.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLCQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="QBA20_001910"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="QBA20_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDQ6292462.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="QBA20_001911"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="QBA20_001911"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDQ6292463.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="QBA20_001912"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="QBA20_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDQ6292464.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /locus_tag="QBA20_001913"
     CDS             7139..7750
                     /locus_tag="QBA20_001913"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDQ6292465.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="QBA20_001914"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="QBA20_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDQ6292466.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="QBA20_001915"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="QBA20_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="QBA20_001916"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="QBA20_001916"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDQ6292467.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12303)
                     /locus_tag="QBA20_001917"
     CDS             complement(11944..12303)
                     /locus_tag="QBA20_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292468.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12300..12806)
                     /locus_tag="QBA20_001918"
     CDS             complement(12300..12806)
                     /locus_tag="QBA20_001918"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292469.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12952..13515)
                     /locus_tag="QBA20_001919"
     CDS             complement(12952..13515)
                     /locus_tag="QBA20_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292470.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13722..14660)
                     /locus_tag="QBA20_001920"
                     /pseudo
     CDS             complement(13722..14660)
                     /locus_tag="QBA20_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14723..15097)
                     /locus_tag="QBA20_001921"
     CDS             complement(14723..15097)
                     /locus_tag="QBA20_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HDQ6292471.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15101..16249)
                     /gene="wzy"
                     /locus_tag="QBA20_001922"
     CDS             complement(15101..16249)
                     /gene="wzy"
                     /locus_tag="QBA20_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HDQ6292472.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16269..17174)
                     /gene="rfbG"
                     /locus_tag="QBA20_001923"
     CDS             complement(16269..17174)
                     /gene="rfbG"
                     /locus_tag="QBA20_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HDQ6292473.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17167..18057)
                     /locus_tag="QBA20_001924"
     CDS             complement(17167..18057)
                     /locus_tag="QBA20_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HDQ6292474.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18087..19343)
                     /gene="wzx"
                     /locus_tag="QBA20_001925"
     CDS             complement(18087..19343)
                     /gene="wzx"
                     /locus_tag="QBA20_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HDQ6292475.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19359..19904)
                     /gene="rfbC"
                     /locus_tag="QBA20_001926"
     CDS             complement(19359..19904)
                     /gene="rfbC"
                     /locus_tag="QBA20_001926"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDQ6292476.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19909..20787)
                     /gene="rfbA"
                     /locus_tag="QBA20_001927"
     CDS             complement(19909..20787)
                     /gene="rfbA"
                     /locus_tag="QBA20_001927"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDQ6292477.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20846..21745)
                     /gene="rfbD"
                     /locus_tag="QBA20_001928"
     CDS             complement(20846..21745)
                     /gene="rfbD"
                     /locus_tag="QBA20_001928"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDQ6292478.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21745..22830)
                     /gene="rfbB"
                     /locus_tag="QBA20_001929"
     CDS             complement(21745..22830)
                     /gene="rfbB"
                     /locus_tag="QBA20_001929"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDQ6292479.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23203..24096)
                     /gene="galF"
                     /locus_tag="QBA20_001930"
     CDS             complement(23203..24096)
                     /gene="galF"
                     /locus_tag="QBA20_001930"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDQ6292480.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24271..25665)
                     /gene="wcaM"
                     /locus_tag="QBA20_001931"
     CDS             complement(24271..25665)
                     /gene="wcaM"
                     /locus_tag="QBA20_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDQ6292481.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25676..26896)
                     /gene="wcaL"
                     /locus_tag="QBA20_001932"
     CDS             complement(25676..26896)
                     /gene="wcaL"
                     /locus_tag="QBA20_001932"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDQ6292482.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26893..28173)
                     /gene="wcaK"
                     /locus_tag="QBA20_001933"
     CDS             complement(26893..28173)
                     /gene="wcaK"
                     /locus_tag="QBA20_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDQ6292483.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28353..29831)
                     /gene="wzxC"
                     /locus_tag="QBA20_001934"
     CDS             complement(28353..29831)
                     /gene="wzxC"
                     /locus_tag="QBA20_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDQ6292484.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29833..31227)
                     /gene="wcaJ"
                     /locus_tag="QBA20_001935"
     CDS             complement(29833..31227)
                     /gene="wcaJ"
                     /locus_tag="QBA20_001935"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDQ6292485.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31282..32652)
                     /gene="cpsG"
                     /locus_tag="QBA20_001936"
                     /gene_synonym="manB"
     CDS             complement(31282..32652)
                     /gene="cpsG"
                     /locus_tag="QBA20_001936"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDQ6292486.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32845..34281)
                     /gene="cpsB"
                     /locus_tag="QBA20_001937"
                     /pseudo
     CDS             complement(32845..34281)
                     /gene="cpsB"
                     /locus_tag="QBA20_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
     gene            complement(34284..35507)
                     /gene="wcaI"
                     /locus_tag="QBA20_001938"
     CDS             complement(34284..35507)
                     /gene="wcaI"
                     /locus_tag="QBA20_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDQ6292487.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLVTAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35504..35983)
                     /gene="gmm"
                     /locus_tag="QBA20_001939"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35504..35983)
                     /gene="gmm"
                     /locus_tag="QBA20_001939"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDQ6292488.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35983..36951)
                     /gene="fcl"
                     /locus_tag="QBA20_001940"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35983..36951)
                     /gene="fcl"
                     /locus_tag="QBA20_001940"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDQ6292489.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36954..38075)
                     /gene="gmd"
                     /locus_tag="QBA20_001941"
     CDS             complement(36954..38075)
                     /gene="gmd"
                     /locus_tag="QBA20_001941"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDQ6292490.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38101..38649)
                     /gene="wcaF"
                     /locus_tag="QBA20_001942"
     CDS             complement(38101..38649)
                     /gene="wcaF"
                     /locus_tag="QBA20_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDQ6292491.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38665..>38786)
                     /locus_tag="QBA20_001943"
     CDS             complement(38665..>38786)
                     /locus_tag="QBA20_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDQ6292492.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10354 a         9714 c         8730 g         9988 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgagcgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgccc gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgtgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggaccaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgaggtatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaata
    12361 atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt
    12421 aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac
    12481 ctgtcacctt gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat
    12541 acccaaagta ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca
    12601 tcactgccaa ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt
    12661 cgagcttgtt ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat
    12721 atatttcctc cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa
    12781 taaatgccac caagaattgt tgccaagcta aaaataagaa atgtctgtat gtttctcttt
    12841 ttcgctatca atataacaaa tatgatggct ccatatatgg caccaaatag ttttacgcca
    12901 caagcaattc caataaaaaa accacataaa aaaattaatt cattttgtgt tttattgttt
    12961 tctgagcgaa tatatataag aatactggct gcagcaaaac aaagatagcg ttaaatcaat
    13021 atatgcataa ccaagatgat ctttaagtgg attgatagta aatagtagga ttgtagcaat
    13081 aatgcctgga taataagttt tcgtaatttc ccatgaacag ccaacaagtc cacaggctat
    13141 tacaaaaaaa cagatatttg ctaaaaactg agctaacctt acatctccta actgtaaacc
    13201 taaagccatt aaaagatctg cattttgcgg gaacagagga aaacgcaaat attgttcaac
    13261 aacaattgca tggtgctcaa ctaatgaacg agccaaaggt aagtgataca tagtatcatc
    13321 ccaatcaccg ggagctcgca gggacgataa caatgtaacc acaaaaatca aaacaagaaa
    13381 taaaataaca aacagcgtac tgaactcaat attatttatt gccagccatc taaattgctg
    13441 acgtttaata atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag caatcttatt
    13501 aaattgcccg accatgccca gaaagtaaat tatcaaaata tttaaagaca tccctatgca
    13561 taggcaataa aaaataaaga aaatttgtta agtcctgcca ccccctcctt aaaaaaagat
    13621 gacaatacaa tattaccaat gacaaaaaat gtcattccaa aagcaaaaca atacacaaca
    13681 tacaaataca acggcaattc aatcatagta aaatactcta tttacattat cagtttatag
    13741 tttttttcag aaagataaaa taatgccttc atttcacgtc tagttgttgt atttgaatgc
    13801 aaaataatgc cacatacaaa tgccattatt gacgataaca tgatggaggc agttaaaaag
    13861 gcagtcggaa atcttctcac caaacctgta tcaatataat caacaatgat gggcaatcct
    13921 aaaattatag acaaagcagc aagtaaaacg ccgataatac taaaaaagta tagtggccgt
    13981 tcaagcgagt aaagtttaat tattgttttc agaatcttga acccatcact ccaagttgat
    14041 agtttgctca cactcccttc agatcgctcg ccatattttg tattaacctc accatacttc
    14101 atacgtaatt ctaaagcatg aattgtaagc tcagtttcga tttcaaatcc ccttgataga
    14161 catggaaaag atttaacata gcgtctggaa aatacacgat accccgacaa catatcagta
    14221 aaattccctt taaagatagt ctgaacagta ccagtcaaca gtttatttcc tcagcgatgc
    14281 ccttttctgt atgtattttg atctccattc tcttgacgac atcctacaac catatccaat
    14341 tgatttttta tgagatggtt aatcatttct ggagcagaac ttgcatcata agtgtcatct
    14401 ccatcaacca ttagataaat atccgcatct acatctgaga acatacgtct tacgacattc
    14461 cccttgcctt tcagaggaac agagtgaact gttgctccag catcctctgc cacttgtgct
    14521 gtactgtcag tcgaattatt atcaaataca tatatagaag cagttggaat attcgttttg
    14581 aaatcattta tgacttttgc tattgccttc tgctcattat aacaaggaat aattacagcg
    14641 atattaaaag agtgatacat aagcccctca tataaactat ttagctaatg tgcaagtaat
    14701 tgtgttttta acaaatcgtt tactaacgaa aaatgaaata ttttgatgcg caatatccta
    14761 aaaccaaact aaaaagagaa aaggtgaaca atgtaaatat tggagataag gctaagagat
    14821 caaataaaac gccgaataaa taactcatcg cccccataaa aaatataaat ataaaatatc
    14881 tagccttggt tgcagatact ttaaatgagc atcttgcatt tgcaaaaaaa gaaaaggttg
    14941 ctgcacatat aaaaccaaca atgttacttc ggccttgacc aaaagctaaa ttattataac
    15001 acgcataaaa taccccccaa tgtaaagcag tattgacaat tccaattaaa aaataactaa
    15061 aaaaagaact ggtcaataac ttccctattt ttaacatcct ttattttgct ccagaagtga
    15121 ggttattact aatttggata ttttctatag aaaataccca taaaactaca acgcctataa
    15181 taaaaattcc catgggtgta aaaagaaatg gattagttat agaaacggta atactgaaaa
    15241 aaagtattaa cgacatcatt aatgatgttt ttgtactatt taaaaaatag cctttcaaaa
    15301 agataagcaa cataggaact aatgatataa atacaccaat aacgcctgtt ttctgaagta
    15361 tctcaagagg tactatttca atccttaatc gatctaatat aaatgaacca aatcctcttc
    15421 caaacaagaa cgtcgctaaa tcaacatttt tataataaaa atataaatca ttaaatctaa
    15481 cagagtcaga atcttcactg cgtgatccga ccatgtaaag cataattata aatagaacgc
    15541 tgcccaatat tattataata gtatttaaat atagcttatg taatttaaaa ctggctaata
    15601 acagtgataa tgttgtaaaa agtaatacac ctcttgattc tgtcaataat atcgcaagca
    15661 ctattatgag acattttaaa tattttttgt cgacaaaact gataatcgca ccgacaccaa
    15721 aaaagtagaa ggatttggaa aaaaaagccc catcgggacg aaaaaagaac tcgctagtca
    15781 atgataagtg ttcataaatt aaattaaaat ttaacaaacc gaaattcaac aaaattatgt
    15841 atgaaatata taacagtgat ccatataatg caaatgcatt aactatttta ataaaaatct
    15901 cacatgaaaa tacttttaaa gtatatctta gtgtaaatag atatataagt gacagcataa
    15961 aaaggtatgg ttgcacatca aacagtatat catttatttt attaccagct aataagccga
    16021 caaagaagcc ataaaaagga aataaaagca accatataaa aagactgaca catttttggg
    16081 tgattgaaaa attgaatgta actaaattaa aaattaaaag tataaaacta aatacaaata
    16141 ataaatatcg caaagggaat attcccaatg gctccagtaa tcttccacca ccaccaataa
    16201 tcagttcaat acataaaaat gttataaaaa ttttatttat attattcata ttcgtaaggt
    16261 gatgtttttt aaaatatgtt taaaatcaac attaataaaa tataaatttt atcagccttt
    16321 ttccgcctta acttccaaaa acttaaagcc aagatcaaat tgcctatata acctttaccc
    16381 aattgattat tcacaaagct atcatcagct aaggcattag ctattttagt tacttctttt
    16441 cgataccact tgtttctcac caaaccaagc cgtttgtatt ttgctttaaa actaatgttt
    16501 gctcccactt ggttgttttc atgctgtctg tacagcattg ttggttttct atcaatatac
    16561 cacgaataat ttctcgtccg cgcaaatgaa tataaaaacc aatcatgcag acagatatct
    16621 tgtgcagcat ttttattatt tatgatgaac ttcttaaatt caataagcgt ttcttttttt
    16681 ataacatatg tacaccccgg acctgctgct tcaaaaaaat aatcgaattg agtttgcgac
    16741 tgagccttat caactaaatt ttttcgccct gatggataat atgcaattac attactggaa
    16801 taacaattcg cattattaaa taccaagcag tcaattgcat tttttatttt atctttatac
    16861 caaatatcgt cctgatcact aaaacagata taatcatagt tttcaagatc gacatcttta
    16921 attaatctat aaaaattttt acctgccccc ccaaacttat tgacgcttgg aagtatattt
    16981 ataattgagg gatatgataa ttgaaattct tcacaaatat ttaatgtatt atcagttgat
    17041 aaatcatctg atataaaaat tgagatgtct acatcttttt gttcaataat actttttaat
    17101 tgttcctcaa tccagcattc gccattatac gtagccataa gtacagctac tcttttttga
    17161 tcagaactat gcattataac gaccgccccc agtaattcct cttattccat ccagcgtagc
    17221 caaaatatgt tttttcatat attcaatttt atttttttga gttgcgataa ttaaagtcgt
    17281 atgaattaaa gaaaaaacaa cttccctaat agagagtaaa agaggcacat gattttttct
    17341 aagcaaaaga aatgcatttc ttactcgata ataacgacgt actggcgagt gaattggtat
    17401 gttaatccca cagattttta ctgacttatc cccaatagca tgaaccattt ctatatcagg
    17461 aatgatatga actaatatgc catagtttaa gcatctaaga caccattctg tatcgacata
    17521 atcaataaaa agttcctcat ccatcattcc aacaatctca aaaacttctt ttgaaaccat
    17581 ggttccggat gagattgtta ctgaactttt aaacggtgtc tgtccttcag tgacatgaat
    17641 tttctctcga agaccatttt ttttgatatt acaaatagga taataatgtg acttatctct
    17701 atcaataaaa actgggccag ctatttttgc attttcatta tccatatagc aggaaagttt
    17761 tttgataaac tcattgcgaa tacggctatc ttgatcaaaa aaaattatct tctctgcccc
    17821 tttttcaaga gaatatcgaa tcccaatatt ttgtgctttt gctatacctt cattcctacc
    17881 aagacaaatt aaatgaactt tttcaatgtc tgctaattta tctttcaagg tgaaattagt
    17941 cctattatcg actactacaa cgtaacaatt ttgctccttt agttcagtga tcagtgcatt
    18001 cagattttta agttcgggat tatatgtcac aatgacagcg taaatattac tattcattat
    18061 ccaagtgact cagtaattgg ttaattttag ttttcatata cagaacacat tctagtataa
    18121 agccattgta ataacagagc atctaaaatt acacgtatgc tccaggatat tgcggcaccc
    18181 agtaacccat atttttcgac cataaaatac aaaagggcta aataaggaac caattcagca
    18241 caatgaatca aagcagtaat tttagatttt cctaatgcct ggattgcaga gaatggtatc
    18301 attgccagcg cattgaagaa aaaaccgatc aataacacat ttaatatatt aaaggaatta
    18361 atttgataat tctcaccaaa ccatattttc aatacaagcc ctgaatataa caagcctatg
    18421 attatcaccg gcaaacagat tagaaacatt aaaagcaatg atttatttac attacgttta
    18481 aattctttaa agtctttgat gttacttaac cttggaaaca ctgccctccc gattgctgca
    18541 ggtattattc ctaattttag aataacctcc gctggtgcag aataaaatgc aactttatct
    18601 gcccccataa tatttgacac tataaatcga tcaaaataca ccatgaccgg acttatgata
    18661 ttacttactg tcatccaacc gccaaagaaa aagagtcgtt taaaggtttt ataacaaaaa
    18721 tgaacccccg aaattttaat atcattcctg acataatagg cagagactaa aatcgaaata
    18781 actcttgcaa atattaaacc agcaaccgct gccgacaatg tagcactata aaaaacaaat
    18841 atggccggaa ttcccgcgat gcaagagctt gatatagatt tttgaatatt tacaatgcca
    18901 aatttttcat ccccctcaag aatggctgac cataattgat ttagaataaa taatggaatg
    18961 caaatagcca acagtttaaa tgctagttgt atatcactat gttcaacacc agaaattttt
    19021 agataattaa caattccatc agagaaaatc aataataaaa aagcaccaaa gcatgaaaat
    19081 agcaccaaaa aagatgtact tgttgaaatt accttggttc tttcatgatg attatcacga
    19141 tgaattgcaa tttctctaat aacagagcga gtcaggccta catcaaaaat tccagcatac
    19201 ccaacaagtg caattgcaat tgtataaata ccgaaacctt caggccccaa acctcgagcg
    19261 agaaatccta gcgaggggat agcaattaaa gttggtattg catagccaaa aaggttccag
    19321 acactatttt ttattatgct cattattaca tttctttatc atataaaata atcagcatct
    19381 acaaattttt gaccattaat atctttatca gaaagcaatg gattttgaac cggccattct
    19441 atatttatac ttttgtcgct ccataaaatc ccacgctctt gcatttttga atagaaatta
    19501 tttgttttgt agacaaattc tgcttcatca ctaagaacgc aaaacccatg agcgaatcct
    19561 tccggtatcc ataattgacg cttattctcc gccgacaaat ttacaccaac ccacttgcca
    19621 aatgttggtg atgatttccg aatatcaaca gctacgtcaa aaacttcacc gacaacacag
    19681 cgtaccagtt ttccttgagc ataaggttcc agctgatagt gcagtccgcg taacacaccc
    19741 ttagttgatt tagaatggtt atcctgaaca aactcaacct ttcgtcctac agcctcttcg
    19801 aaaactttct gattgaagct ttccataaaa aagccccgct catcaccaaa tacttttggt
    19861 tcaaaaatta gcacatcagg aatttcagtt ttaattacgt tcattttatt aataaccttt
    19921 aatcattttc agcagatact gaccataagc atttttcttc agcggctcgg ctaatacctt
    19981 cacttgctca gcatcaataa agcctttacg gtaagcaatt tcttccgggc aggaaacttt
    20041 cagcccctga cgctcttcaa ttgttgcaat gaagttgctt gcttcaataa gactttgatg
    20101 tgtccccgtg tccagccatg cataaccacg ccccatcata gcgacagaca aacgtccttg
    20161 gtccatataa atacggttaa tatcggtaat ttccagttcg ccacgtgcag atggtttaag
    20221 gtttttcgcc atttctacaa cgtcattgtc atagaaataa agcccagtaa ccgcatagtt
    20281 actttttggt tccagcggtt tttcttccag actaattgcc gtaccgttat catcaaattc
    20341 caccacaccg tagcgctcag gatcatttac gtgataggca aaaaccgttg cgccactctc
    20401 tctgttaaca gcggtatcca ttaatttcgg caggtcgtga ccatagaaga tattatcacc
    20461 aagtaccagc gcacaatcat caccacctat aaattcttca ccaatgataa atgcttgcgc
    20521 cagaccatcc ggactcggtt gtactttata ttgcagatta agcccccact ggcttccatc
    20581 accaagcaat tgttgaaaac gtggtgtatc ctgcggcgta ctgataatca gaatatctcg
    20641 aatacccgcc aacataagcg ttgaaagcgg ataataaatc atcggtttat cataaatcgg
    20701 tagcagttgt ttgctcacag ccattgtcac aggataaaga cgagtgccgg aaccaccagc
    20761 cagaataata cctttacgcg ttttcatttc accattcctt ttaattcatc tcgctctagc
    20821 atcatgagcg agatgcaata atttgttaaa ttgccgtggt cgtaaataat tcgttgagca
    20881 tacgtttcac gcccacctgc cagtcaggca agacaagcgc aaagttctgc tgaaattttt
    20941 cggtattgag gcgagaatta tggggacgac gtgctggtgt gggataggcc gttgtcggca
    21001 cggcattaag tttgttaaga gcaaggttga tccccgctct gcgcgcttct tcaaatacca
    21061 gagcggcata atcgtgccag gttgttgtac caccagcaac cagatggtac aaaccagcaa
    21121 cttctggttt atttgcggcc acgcgaatgg catgagcggt gcaatcagcc agtaattcag
    21181 cccccgttgg tgcgccgaat tgatcgttta ttacagccaa ctcttcgcgt tcttttgcca
    21241 ggcgcaacat cgttttggca aagttatttc ctttaccagc gtatacccag ctggtacgga
    21301 aaataagatg ctttgcgcaa tgttcttgta acgctttttc cccagctagc ttggtttcac
    21361 cgtagacatt tagcggtgct gttgcatccg tttccagcca tggcgtgtca ccgttgcctg
    21421 ggaaaacata atcagtggag tagtgtataa cccaggctcc gacttcatta gctgcttttg
    21481 caatcgcttc gacgcatgtc gcgttaagta attgcgcgaa attcggttct gattccgctt
    21541 tgtctactgc agtgtgagca gcagcattaa caataacatc ggggcgaatt tttttgacgg
    21601 tttcagccac accttcgggg ttgctgaaat ctccacaata atcagtggag tgaacatcga
    21661 gcgcaatcaa gttacccaga ggagccagag cacgctgcag ttcccaaccc acctgccctg
    21721 ttttgccgaa aagcaggata ttcattactg acggccctca tagttttgtt caatccacga
    21781 ctgataagca ccgcttttca cattctcaac ccaatttgta ttagccagat accattccac
    21841 cgttttacga atcccgctct caaacgtttc ttgcggtttc cagcccaatt cgcggctaat
    21901 tttatcggca tcaatggcgt aacggcggtc atgccccggg cgatcggcaa cataagtaat
    21961 ttgctcacga taagatttct ctttcggtac aatctcatcc aacaaatcac aaatagtgag
    22021 cactacgtcg atgtttttct tttcgttatg tccaccaatg ttataagttt cacccgcttt
    22081 accttcggtt acgacggtat ataacgcacg agcatgatcc tctacataca accagtcgcg
    22141 aatttgattc cctttaccat aaataggtaa tgccttacct tccagagcat tcagaataac
    22201 caatggaata agtttttccg ggaagtgata aggaccataa ttgttcgagc aattagtcac
    22261 aatggttggt aaaccataag tccgcttcca ggcacgtacc aaatgatcgc tggaagcttt
    22321 agacgcagaa tacgggctac ttggcgcgta agctgtcgtt tccgtaaata gcggtaacgc
    22381 ttcgttatta tttgcttcat ccggatgggg taagtcacca taaacttcat cagtagaaat
    22441 atgatgaaag cggaaacttt ttttcttttc atcattcaga gcagaccagt aattgcgcgc
    22501 cgcttctaaa agaacataag tccccacaat attggtttca ataaatgccg cagggcccgt
    22561 tattgaacgg tcaacgtggc tctctgccgc caggtgcatc accgcgtctg gctggtgctg
    22621 tgcgaaaata cgcgacatgg ctacagcatc gcagatatct gcatgctcaa aagcataacg
    22681 ttcagaatca gaaacatcag cgagtgattc caggttcccg gcgtatgtta atttatcgac
    22741 attaacaaca ctatcttgcg tattatttat tatgtgacga acaacagcag aaccaataaa
    22801 ccctgcgcca ccagtaacaa gtatcttcac ttttttattc catatagcta tagagcatgc
    22861 tgtgaaatag actgctccag atttgattaa tagaggtatt aatacacgct accgcccctg
    22921 gcttaacagc taccagtgca ctgcatgcat ctcaacgatg tggcgagcgt tagccactcg
    22981 cgccaaatcc gaaaaattca aacgttaatt gtcttaccaa acagccgtag aaacaaggaa
    23041 aatcctggaa aaatttgaat aatatgagct atgtaacttc ttgtttttat aggctttata
    23101 aaaacggcaa cggcaggaaa attcaatgag caaaacttat gccacgaatc ttcactgccg
    23161 ttataatttt cttatcaacc gttacatccg gtcagatttt cattattcgc ttaacagctt
    23221 ctcaatacct ttacggaact tcgccccttc tttcaggttg cgtaggccat acttcacaaa
    23281 cgcctgcata tagcccattt ttttgccgca gtcgtaactg tcgccggtca tcagcattgc
    23341 atcaacggat tgttttttcg ccagctcggc aatagcatca gtcagctgaa tacgtcccca
    23401 tgcaccaggc tgagtacgtt ccagttccgg ccaaatatcg gcagaaagca catagcgacc
    23461 tacggccatg atgtctgagt ccagcgtctg cggctgatcc ggtttttcga taaattcaac
    23521 aatgcggctg actttaccct cacggtccag cggctcttta gtctggatga cggagtattc
    23581 agagaggtca cccggcatac gttttgccag cacctggctg cggcccgttt cgttgaaacg
    23641 tgcaatcatg gcagcaaggt tgtaacgtag cgggtcggcg ctggcatcgt cgatcacaac
    23701 gtctggcagt accacgacaa atgggttgtc accaatggca ggtcgcgcac acaaaatgga
    23761 gtggcctaaa cctaaaggtt cgccctgacg cacgttcata atgatcacgc ccggcggaca
    23821 gatggactgt acttccgcca gcagttgacg cttcacgcgc tgctcaagga gtgattctaa
    23881 ctcataagag gtgtcgaagt ggttttcgac cgcgttcttg gacgcgtgag ttaccaggag
    23941 gatttctttg atccctgcag ccacaatctc gtcaacaatg tactgaatca ttggcttgtc
    24001 gacgattggt agcatctctt tgggtatcgc cttagtggca ggcaacatat gcatcccgag
    24061 acccgctaca ggaataactg cttttaaatt cgtcattatt tcatccacct gtaaaatggt
    24121 tgctgaatta tagcttgttc gatttttttc gccagcatca attaccctga attgattact
    24181 gaattacttg tgatgttacg ccgcttcgtt gtggattgca gtagcattgt tcctaagtat
    24241 ggctccattt ttccaggaat ggtcgcaaat ctactccctc cgttccggca atctaaagtt
    24301 aatcttttcc acattaacaa tatggtgatt aatcctgtcg atatcgacgg agctttgccc
    24361 tctttcattc accgcatgaa catttgcaag agacagcagt gtttcttttt tcgccataaa
    24421 gacgccgcga acgtctttgc gcatgtcgaa gttcatgctc aatgctggtc caactgagga
    24481 ttcctgcatc acattgatat ttcgcataaa aagatgttgc ggcttgttgt gtaactccag
    24541 cgaggcacgc ttcatctcaa tgttagttag cgacacaaag gagacggcat tcccggcgga
    24601 gatttggatg ccgcgcaatt tataagcaag atgggtatta tccagttgaa tattattcac
    24661 tcggaaattt tgcggtatcg agagatattt gcctttaatt accccatagc cgattaacat
    24721 cccggcgcta ttaatcattt caatattatc aatcacgaaa ttgtcacaac cgtaaatagc
    24781 gactgtcgcg ttatcaatgc ccgctttctt actgaaatcc ggcgtgatat tgcgggcatt
    24841 gatattacga ataacaaaat gtttaccatt ttcaacatgg atcaactgcc gacaatccga
    24901 tcccgtgata ttcgccacga caaagttttt cactgcctgg tcttccgggt agttgttatc
    24961 ataagtgctt cccgcaaggc ctatgccgat gccccagttg attttgccgt tggtacagtt
    25021 gatacgctcg atgacatggt cagatatcaa aatatcactg tcgttaattg ccacgttcca
    25081 ttcaatggcg tcgccctgta agtcgctgaa cttacagttg gtaatgttgg caccgataat
    25141 ctggttatga aatccctggc gtaagatggc gtaattagcg tggctgacag tgaggttatc
    25201 gatggtcagg ttgcgcatga cccgtttgtt tttgccgcca atataaatct gcattaccgg
    25261 gccaaagccg ctcatcgcca gccctttgat ggtgcagtca gaaccacgca catccagggt
    25321 aatgttatgc ataccgccgc cctctccccc tgtcacctgg ctgccgtcct gtaagacaaa
    25381 tcgccctctg ccgttgccgc gcaggcttcc aaggatgtgt aacgttttgc cgggagggat
    25441 gaagatgccg gtgttgatat tgtcacaaac gaatccggca ggcacgacga ccgtttgccc
    25501 ttcgctgaag gcttgtttaa atgaggcgat ccagtcatgt gggttgtagt cgttaatgtt
    25561 aacgctttgt cgggcgggaa gtgcgcgagc gaaaggggta tggaggaagg caagcgccga
    25621 gcttgccgtc aggaaggtgc gtcgggagag tgttttaaat ggcatacgtt ctcctctata
    25681 aagcctgcag caagctggcg agttctcgat taatcacctg ctggttaaaa tcgtgttcga
    25741 ctttttcgcg cgcacgtttg acgaccggag ccagttcgtc ggtatccagt tggctaaacg
    25801 ccgccagacg ttgtgccagt gcgcgagcat cgttctcagg cagcagccag ccggatttat
    25861 cggcctccac cagttcagga attccgctat gcagagtaga aaccaccgga ataccgaccg
    25921 ccatcgcttc cattagcgcc accggaatgc cttccatatc gccatccgcg cccgttaccg
    25981 atggcaacag gaagacatcc gcgtcgtcga gcatctcctt cacttcatgg ctcggtttaa
    26041 agcccggcat ctctaccaca tcttccagtt gatattgttc gatgagggtg cgcaggcgtc
    26101 gttcccacag gccaatgccg aggatgcgat agcgaaatgc cacgccctgc tctttcaact
    26161 gacggcaggc ttcaatcgcc acatgcaggc cttttttctc ggttaagcgt gcgacagata
    26221 tgatttccag cggcgttgcg ggcgctttca ccggacgcgg gctaaagcgc gtcatgtcca
    26281 cgcccatacg cgatacggcg attttttctc tggggcagcc cattttttgc agccttccgg
    26341 cccacagatc gcttatcggt aacatcaggt cgccacggcg aaacagttgt tgatattcgg
    26401 gagtgtagtg gttgagcact tcccgactgg agatatcaat accgtggaag atggtggcaa
    26461 ttttgccgcg aatgataccc agttcgcgta gttttgctgc ggttaccccc gccgggccaa
    26521 aatgagcgat gaacacatcg gcgcgaaacg gtgttgcgac ctgaccacaa atggcagaca
    26581 aaatcaggtt ccgcgactcg gcaccatagc gtttgaggtt gagcgcatgc caggtatttt
    26641 tacgatgaat gccacgtaag gtctggctgg cgcggtggcg cagtttcgct actttgcccg
    26701 caggttcgtc ctgtaaccag cgggttctgg cgacaaggtt gtatttcgtc catgccgcgt
    26761 gggtgttctc tgtatcgcct ttttgcaaag cgacaatctc cacttcaaag cccatatcaa
    26821 taaacgcggt aatttgattg aggacgaagg tttccgacga cagcgggaat ttcaataaga
    26881 agaagccgac cttcatttca cctccccgat gcgctcaagc acagactgca ccatctgcat
    26941 tcccgtctga cgttcgcgac tgacggcttc gttaaatcga gcgttcagca ccggaagctg
    27001 gcctaaggta tccgcaacca tcgcttgcag gctgccgtcc agtaaatgac gaatatcaat
    27061 cgccatctct ggcagtccca gctgctgcat aatcccggcg gatttatgtt catagttgat
    27121 ggcaatcgcc ggagtagcaa agttcattga gataatggcg gagtgcaggc gcgtaccgac
    27181 ggtgagttca caggctccga gaattttgcc catttccaga tcgttgagtt catccatcac
    27241 tacgtggtaa cgggcaggat cgctgatgtg ctggcgcaga ttgagcgcca ccatgcggtc
    27301 gtctttgtta tagctgtcga tacccgtaca ggtggagagc gcaatcacct gatagccttc
    27361 atcgagaatg cgattgacca ccccggcaaa ggctttttca tacgcttgtt gagtggtgcc
    27421 gagacgtttg tcgaacggtg ccagttcgcg cagggtaatt gccactgttt tctgttgtgc
    27481 ggcaacgtcc agccagtgtt gaacggcata gctggcggtg aagtcttcgg tgtggtgatc
    27541 gaccagccac gcggtatcga cgccatgttc cacttttgcg gtggtgatat ttctgcgttt
    27601 catcaaatca aggctgaccg attcgcgcag gatcagcgcg tcgcagtgac caaagacata
    27661 gttcgccagt tggttaaatt gctcatcctg gaaggggccg acgctgtgac caatcataaa
    27721 cagcggcttt ttcgccataa atgtgcaaag cgcatgttca aactgcggca cgccgtagag
    27781 atcgacaaaa aacgatccgc cgacctgaat aatggcgtcg taacctgaca gcaggcgcac
    27841 gaagtcggtg aatccctggg cgatggcgat attgcgcagc ttgccagtgt cggtgacgcg
    27901 tgagagcaat acctgatgct ggtagcggcg acggaggact tttttaatgc gcccgacaac
    27961 gcccgccgcg ctattgtgtt gtgtcatctg caggaacagc ggatcgccca ttaccgggcg
    28021 gttgagcagc caggaagaac tgaccggata gcggctcatc acgtccactt cggtatgtgg
    28081 attgaggatg ttgatggcat caagtaagcc gcgcaagatg gcgctgtcgc cacgattgcc
    28141 gcaagtgtgg ttgcccagaa taagtaattt cattttttcc tcataaattt gatgccaggt
    28201 gaggctgtgt ttgttgtgtt cgttgccgtt ttttgcctga tgcgacgctg gcgcgtctta
    28261 tcaggcctac aggtcccgag cacagaaccg taggacggat aaggcgtttt acgccgcatc
    28321 cggcaaccgt tgtcggaacc gaaaacagca attcaccccg cccgtaaaag cattttcatt
    28381 ttttcgctgc gacaaaactg acgcttcact tccaccacca gcggatggcg tgacagcaca
    28441 atcatcacca caaacgccag cacgcccgcg gctatttgca ccgccagcag catccccagc
    28501 gccagttgcc ctttcagcac aatgcccagc gcataactga ccaccagcgt cggcaacgag
    28561 agataaaacg gcagccacaa actcaggatg tactggcgat aactggaacc aagaaccggt
    28621 ttaatcatca cgaagtaact cagaatggtg ttgataatct gcaccagcag gaaaccgagc
    28681 gtgacgccga ttgcgcccgc catctgccca ccaataacaa tcgccggaat aaacagaaat
    28741 gttttgaata cgttgaattt aaagctgata tcgacccgcg ctttcgccat cagcagcgaa
    28801 ccaatcgggt tacccacgga gcgcagcaga cccacaatac acagcaattg cagcaccgga
    28861 ataatgctgt tccacttttc gccgaatacc agcggtacga agttattcga caccaccatc
    28921 agccccagca gcgccggaaa gttgataatc cccactatcg acagcagctt gtagaagtta
    28981 acacgcagct tttcggtgtc gtcctgaatt ttggcaaacg ccggaaacaa cacgcgggtg
    29041 atgattgggt tcagcttcat cggcggtacg acggccacgt tgtacgccag gttgtatccc
    29101 cctgccacgc ccgcgccaag aatacgcgcc agcaccagcg ttgaaaggtt ggtgttgaga
    29161 tagttgatga tgctgtccgc cgtcagccag gcaccaaaac gtaagttcgg tgccacagac
    29221 gccagcgaga agtgcagacc ggggcaataa attttgcgtc caaagtagcc aaacagcagt
    29281 gttctcaccg cactattgac cagataaccg aggatcgcgg tcatcgccag cggccagaaa
    29341 tgggcgctaa ctaccgtaaa agtgaagccc gccagcaccg cgctggtttc gatcatgccg
    29401 attttgttga actccagctc cttctgcatc aacgcgcgga actgttgccc gtgggggatc
    29461 accacaaacg ccagcgacaa tgttttaatc aacggtgcca ggtccgggtt attcagcacg
    29521 tcgccgatga catcactcaa caaaaacacc gccacacata ccacaagccc cagcccgacg
    29581 ttcagccagt acagcgtggt gagttcaagg tgactgattt cttttcgctg aataatcgag
    29641 ttagcgatac cgaagtcaga aagcgtatct gccagcgcga taatcaccag cgacacagtc
    29701 agcaggccga actggtggtt gtcgataatc cgcgccagca cggtcatctg caccagcccg
    29761 aggccgatga tgatcaccgt ggcaatagcc gaccacttcg cgccgctgat ggttttttca
    29821 cgtaagctca tatcaatatg ccgctttgtt aacgaagccc ttgaataccg tcaggaaaac
    29881 gattttgata tcgaaccaga cgctccattc gcggatgtac tcaaggtcga actcgacgcg
    29941 tttttccatt ttctccagcg tgtcggtttc gccgcgccag ccattaatct gcgcccagcc
    30001 ggtaatgccc ggtttcactt tatggcgcag catgtagcct tcaatgagct ggcgatactg
    30061 ttcgttatgc gctactgcgt gcggacgtgg accgacaatc gacatccccc cggtcagcac
    30121 attgataaac tgcggcaatt cgtccagcga ggtgcggcgc agaaagttcc ccactttagt
    30181 gacgcgcgga tcgttctgcg tcgcctgggt caccactttg tcgttctcca tcactttcat
    30241 ggaacggaac ttccacactt tgatcggctt gccatccata ccatagcgag tctggcggaa
    30301 aataaccggc cctggtgaac tgagtttcac cgccagcgca atacagcaca gcaccgggga
    30361 gatcagcagc agaataagcg tcgccagcac aatgtcttcc gcacgtttta gcaggcggtt
    30421 aacccccgaa agcggcgtgt catacagcgg caccaccgga acgccgttca tctcttcgag
    30481 acgtgaatgg agaatgttga aggtaaagac gtcggggatc agcagcaccg aacaggtggt
    30541 atccgccagt tgatggacca gttttttcac tcgtgcgccg tcgcacattt gcatcgcgat
    30601 atagacgtta tgaatcttgc ccgcttttgc atcctcgacc agctgttgca ggttacccgc
    30661 ccagtcgtta gaaacgccgc ccggtttcgg gtcgtggtaa acgcccacca cttcaaaccc
    30721 taaccacggc tgattacgga aactctccat cagcatttgt ccggcggcta aatcccccgc
    30781 tacggcgacc atccgcttgt tgtagccatg attacgcagc cagcccgccc caatgcgaat
    30841 acatgaacgg caaaccacca gtccgatgct ggtcagccca taccacgcca gccagatttt
    30901 cagttgcgtg tcgaaatcat tgttgaacgc caccagtccg gcgctgaaaa tcacgcttaa
    30961 ggtccagttt tgtagcaaca gggcaaattc tgtcgctgcc cgaacaccgc gccatgagcg
    31021 ataaaaatcg gtgatgccgc ccagcatctg gaacaccacc agcgtaatca gcgccaccaa
    31081 caggtgcatg tagaggaatg acagtccgct gacttcgcaa accagccata gcccggcaaa
    31141 catgatggtg atatctgaaa agcgttgcac catagagatt aacgatgcat tggttttcgc
    31201 tcgctcgcgc ttttttagat ttgtcatcgt tgttcctgtt attagcccct tacccgcagt
    31261 ggggtaaggg aagatccgac attactcgtt cagcaacgtc agcagagttc gcgttcgcgc
    31321 ttccatcagc ggcacatcac cgcgcgattc cacattcagg cgcaccaccg gttcggtatt
    31381 ggaggtgcgc aggttaaagc accagtcggc aaaggtcatg ctgatgccat cgctgcgatc
    31441 caccgccagc gcctcacggc taaaatgctg ttccacgcgg ttaatcgcct caacgggttg
    31501 cgccagtttg ctgttgatct caccgcttgc cggaaacgcc gccatccggt cgcgcaccag
    31561 ttcgcccagc gtttttcctt tcaggcacac cagttcggcg accagcagcc acgggatcat
    31621 gccgctgtcg cagtaagcga aatcacggaa gtaatggtgg gcgctcattt cgccaccgta
    31681 gatggcgtct tccttgcgca tccgttcttt aataaaggcg tgtccggttt tcgacattac
    31741 cggcgtgccg cctgcagcag tcaccacatc aatagtgttc caagagagac gcggatcatg
    31801 gatgatcttc gcgccgggat ttttttcgag gaacgcttct gccagcaggc cgacaatgta
    31861 gtagccctcg ataaactgcc ctttttcgtc aaacagaaaa cagcggtcaa aatcgccatc
    31921 aaaggcaatg cccatatccg cgccgtgttt gatgaccgca ttgcgggtgt cgtcgcgaca
    31981 ttccggcagc agcgggttag gaataccgtt ggggaaattg ccgtccggtg tgttgtgtac
    32041 tttgattaat tccacgggcg cgccgagggc tttaaagcgg gcttcaatgg cgtccaccac
    32101 cggacccgct gcgccattcc cggagttgat caccagcttg agcggcgtga gatttttgac
    32161 attgatataa ccgaacaggt gatcaacgta agcgtcacgc aggttgattt gctgatagcg
    32221 accgcgtttg gtttcatcga cgggaggaaa gtcgttggct tcagccagac gctggacgtc
    32281 gcgcagtccg gtatcaccgc tgatcgtgcg agccccctcg cgcaccagct tcatgccgtt
    32341 gtaatccatc ggattatggc tggcagtaac ttcaatgccg ccatccacgc cgagatggaa
    32401 cgtggcgaaa tagatctctt cggtgccgga cataccgata tccagcacat cgacgcccgc
    32461 atcctgtaaa ccttttgcca gcgccagttt taaggtttcg ctggtgagac ggacatcacc
    32521 gcctaacaca atggttttcg gtttgagaaa ttcgccatag gcgcgaccaa tgcgccaggc
    32581 gatatcttca ttcagttctt cgcctaattt cccgcgaata tcataggctt taaagcaggt
    32641 taattttttc atgtcgttac cctttttcag gcaatagttg gccctgaccg aaacggccaa
    32701 ttttattttt gtcgttattt acaccgcggt ttcgcattca ttgcctgatg cgacgctgcc
    32761 gcgtcttatc aggcctacaa aaccgaaccg taggtcggat aaggcgttca cgccgcatcc
    32821 gacaaacagc gcctgatgcg acgtttacac ccgtccgtag cgatccgcga agcgcaccac
    32881 atcatcctct tcgagataag agccggagcg cacttcaatt aaatcgagcg gaattttccc
    32941 cgggttttcc aggcagtgcg tcgcccccag cggaatataa atggactcgt tttcaccaag
    33001 cagtttgata tcaccatcaa tggtgacttt tgccgttccc gcgaccacta cccagtgttc
    33061 cgcgcggtga tggtgcatct gtaccgacaa accctcgccc ggtttcacgg tgatgcgttt
    33121 cacctggtag cggtcgcccg cgtcgataga gtcatatttg ccccacggac gatacacttc
    33181 gcgatgcacc cgatgctcat ggcgaccatc ggctttgatc tgctcgacca cttttttcac
    33241 atcctgtacc gcgttacggt cggcaatcag caccgcatct ttggtctgca ccactaccag
    33301 atctttcacc ccgacggtgg tgaccaggcc tgattcggca tacacatagc tgttttcagt
    33361 tttgtgatta atcacatcgc cgtggcaaac gttgccctcg gcggtgtggg cgctgatctc
    33421 ccataatgaa gaccaggaac caacatcgct ccagcccgca tccatcggca ccacaacagc
    33481 atctgccgta ggttccatga ccgcgtaatc caccgactct tccgggcagg cgagaaacgc
    33541 ttcttcatcc acacgaataa aatcgagatc cggatcgacg gcgctcatcg ctttttcgca
    33601 ggcatcgagg atatccgggc gatatttttt cagttcttcg agatagcgtc cggcgcggaa
    33661 caggaacatg ccgctgttcc agtaatattc gccgcttgct acataggcct aggcggtttc
    33721 cagattcggt ttttcgacaa actgcgccac ttcaaaggcc accgtatcct gctcacccac
    33781 agacacttca ccgcgacgaa tatagccata accggtttct ggtagatccg gcacaatgcc
    33841 aaaggtcacc agcttgcccg cttcggcata aggcatggca ttacgcacgg cggcacggaa
    33901 cgcgtcttca tcggcaatca catgatccgc cgccaatacc agcattaacg ggtcgctctc
    33961 cgggctatga cgttttgccg ccagcgccgc cagtgcaatg gcaggtgcag tgttacgccc
    34021 tgccggttcg agaataatgt tctcggtgag tttgttcagt tgacgcagct gttccgcgac
    34081 aataaagcgg tgctgctcat tgcaaatcac caccgggctt tcgcactcca cgccgttcag
    34141 gcggcagatg gtggtttgca gcatggtgag atcgcctttc aggcataaaa actgcttggg
    34201 gtaaagtacg cgggaaagcg gccataaacg gctaccggag ccacctgcca tcacaactgg
    34261 atagagtttc gactgcgcca taattatccc cgaatatcat ttataaattg acgtaacacg
    34321 ttctctttat cgagcgtgcg ttcggcatat tcacgtgcca ccgtgttatg tttgggcagc
    34381 aggagcgcct gacgaatccc cgccaccagc gcctcgaccg attccggttc tacgcaaacc
    34441 gcaatacccg gaaaggtttc gcaaagctgc cccagttctg tgtgggcctc tgcggtaatc
    34501 accgcgttac cgcctaccgc cagaatattg gtcagtttcg acggcaatac ggcatctgcc
    34561 gcgccgcgtt tttgcaccac cagatggcaa tcgcccatct tcagcagtgc aggtaaagcg
    34621 tcatacgatt gcagcgggaa aaattgcatg ttgcgcagtc cacgctgctg cgccattttt
    34681 tccagccgcg ctttgccgcc gccctgcccg acaatggcaa aaatcagcgg ttcatcgcgc
    34741 aggagatcgg cagcttcaat aacgttttcc agcccctgct tttcaccaat attgccggag
    34801 taaagaatga tttttttgtt atccggcagg ccaagctggt tacgaagggc atcaacatcg
    34861 gcgtctgcaa catgctgaaa acgggcaatt tccgaccagt tggggaagaa gatgacgtta
    34921 tccgccgcca cgcctttttc gatggcttta ttcatcatcg aacgcgaaat cgtggaaacg
    34981 ttatcgacgt tatgcagtcc gctacgttcg aacgccgtta ccagctgtgc cactttgccg
    35041 cctttgcctt ttccggcaag gcccagcccc agcatggcgt ccacttcgta atcctgaata
    35101 tgcagcacgg tacgcgcacc agagagcttc gccagcaggc gcattcccgg cgtgcaaaac
    35161 agcgttggca ctacgccaat aatgcgatcc ggcttccagc gacgttgcgc catcagtgga
    35221 aagaaactgc tgacggcaaa actgccgaga tgcaacaagc gtttcagggt gctcggctgt
    35281 tttggcacgt acagcgggca gcgccacacc gtggcagccc cttcttctcg tttgtagcgc
    35341 caggcggaat agttctcacc cacctgccat tgcgggtagt aaggcggtgc ggtaatgacc
    35401 cgcacctcat gaccttgtgc cgccaaccat tccaccatct cgccggtgta tttgccgatg
    35461 ccggttaact ccggcgagta gttaatgccg tagaccagta ttttcataat ccgggtactc
    35521 cggcacgctt ctcagcgaga aaataggctc ggctgttagc gtgaacatcg ttactggcga
    35581 gcagcgcgtc cggcgtcagc cagcggtaat cgtcatgctg ctcatccggc agtaacagct
    35641 cttcttccgc tactctgaag cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc
    35701 cggagaagtt atcgtcataa aaatgctgcc agacaccgta aaactggcct gctgttatcg
    35761 gcagacgcag ccccagttcc gccatcgtca gccgctcaaa tgcggcttcc agcgtttcgt
    35821 ctttctgcac gcgccctccc ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt
    35881 tgccaagcag aaactcgccg cgactgttct cgacaataaa gtcgagagag acaagcggtg
    35941 tggagcgcac tacagtggca aagtcttcct gacgtaaaaa catcattccc cccgaaagcg
    36001 gtcttgattc tcaaggaacc actggtaagt gctggcaagc cccgcttcca gtgagatttc
    36061 gtgataccag ccaagctgat gcaggcgcgt cacatccagc agtttgcgcg gcgtaccatc
    36121 cggtttgctg gcatcaaaaa ccacccgacc tttgtaaccc accactttgg cgatggtttg
    36181 cgccagctcg cggatagtgc agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg
    36241 ttgggtattc tccagccaga cttcatgcgc cagctccatg acatgaatgc tcgccgccgc
    36301 catatcatcg acgtgcagga attcacgcat cggtgtaccg ctgccccata ccaccacgtc
    36361 cggcgcattc tgtgccgtcg cctcgtggaa gcgacgcagc aatgctggta tcacatggga
    36421 gttactcggg tggaagttgt catgcggccc gtacaggttg gtcggcatga cagtgcggta
    36481 atcgcgtcca tactggcggt tgtaagattc gcacagtttg atcccggcga ttttggcaat
    36541 ggcgtaaggc tcgttggtcg gctccagcgt gccctgcaat aactcgcttt ctgccatcgg
    36601 ctgttttgcc agtttcgggt agatgcagga cgatccgaga aacatcagtt tgttcacgtc
    36661 gttctgatgc gcggcgtgaa tgatgttgct ctcaatcatc atgttctggt agatgaaatc
    36721 cgccggatag gtgttgttgg caacaatgcc gcccactttc gccgccgcca gatagacctg
    36781 gtcaatgcgt tcgctggcaa agaaatcatg cacggcgcgg ctgtccagca ggttcagttc
    36841 atcgcgggta cgtaatacca gctccacatc accgcgctgt tcgagctgcc gcttgatggc
    36901 agaaccgacc atcccacaat ggccagcgat aaaaatgcgt tgtttactca tgcttatgac
    36961 tccagcgcga tcgccacgtc gtagccgtga gatttcagca gagagtgttt tttcgccgct
    37021 tcgaggtcat tagccaccat ttcagacacc atctctctga gggtgatttc cggtttccag
    37081 cccagttttt cgtgcgcttt ggtcgggtcg ccgagcagcg tttcaacttc tgccggacgg
    37141 aagtaacgcg gatcaacggc gataatcaca tcacccggtt taacgcccgg cgcgtcatgc
    37201 ccggtgacgg aaaccacaat gcccttctct tcaacacccg tgccttcaaa gcgcagtttg
    37261 atgcccagct gtgctgccgc catttccaca aactgacgta cggagtactg aacgccggtc
    37321 gcgataacga aatcttccgg ctgttcctgt tgcagcatca tccactgcat ttttacgtag
    37381 tctttggcgt ggccccagtc acgcagggaa tccatattgc cgaggtacag gcacgactcc
    37441 agcccctggg cgatgttggc gattgcgcgg gtgattttgc gggtaacgaa ggtttcgccg
    37501 cggcgcgggg attcatggtt gaagagaatt ccgttacaag cgtacatgcc gtaggattcg
    37561 cggtagttaa cggtgatcca gtaggcgtac agtttggcga ccgcatacgg agatcgcggg
    37621 tagaacggcg tggtttcttt ctgcggaatt tcctgcacca gaccatacag ttcagaggtg
    37681 gaagcctgat agaaacgagt tttcttttcc agaccgagga agcggatcgc ctccagcagg
    37741 cgcagcgtac ccatcgcgtc gacgtcagcg gtatattctg gtgactcaaa agagaccgca
    37801 acgtggctca ttgcgcctag gttgtacact tcatccggct gtacttcacg caaaatgcgc
    37861 gtcaggttag aggtatcact caggtcgcca taatgcagat ggaatttcgg gttgcaggtg
    37921 tgcggatcct gataaatgtg atccacgcgc tcggtgttga atgacgatgc gcgacgctta
    37981 ataccatgca cctcgtaacc tttttccagc agaaactctg ccaggtaaga accgtcttgt
    38041 ccggttacac cggtgatgag agcgactttt gacatgtctt attcctctgt atttttgaat
    38101 ttattcagtt tcaacgcgtt cgcgtatcac cactgcgggg ttcccccggc aaaccacatt
    38161 tgccggaagc gatttaaaaa cactgcttcg tgcacccacg acggtgccgt cgccgattgt
    38221 gacgcctggg gcaacaaaga catcggttgc cagccagcat ttctcgccaa tcacaatagg
    38281 cgtggcgtta atggtgaaat gttgacttgc atggtcgtgg ctaccggtgc ataaataact
    38341 tttttgcgat atcaccgaat gtgcgccaat ggttatttca ccgagggtat ataaattgac
    38401 gtcatcgcca acccacgcgt aatcacctaa ggttaatttc cacggatagg taatttttac
    38461 tgacggacga ataactacgt tttttcctat ttttgctccg aataaacgta ataaaaaagc
    38521 ccgccagcga tacaatactt gtggcgacca ggcaaatatt gttgcctgta ctgcccacca
    38581 taattgcact ttaatagcgt tgccgccccg gaaccctttc ggcaccgaga aaccgcttaa
    38641 atcttgcatt ggttttcctt atattcagac tttgttatat aaggctttag tcttacctgt
    38701 cgtacgttgg cgtaaatgcc aggataattc tgcccagaag ccaggtacat gtaatatctg
    38761 ccgttggact tttttggcgt cagcac
//
LOCUS       DAOFMX010000034        38436 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.034, whole genome shotgun sequence.
ACCESSION   DAOFMX010000034 DAOFMX010000000
VERSION     DAOFMX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.034"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..74)
                     /locus_tag="QBA20_001944"
     CDS             complement(<1..74)
                     /locus_tag="QBA20_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HDQ6292493.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="QBA20_001945"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="QBA20_001945"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDQ6292494.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="QBA20_001946"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="QBA20_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDQ6292495.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGSGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="QBA20_001947"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="QBA20_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDQ6292496.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="QBA20_001948"
     CDS             2704..2940
                     /locus_tag="QBA20_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDQ6292497.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="QBA20_001949"
     CDS             2943..3800
                     /locus_tag="QBA20_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDQ6292498.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="QBA20_001950"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="QBA20_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="QBA20_001951"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="QBA20_001951"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDQ6292499.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="QBA20_001952"
     CDS             complement(6415..7311)
                     /locus_tag="QBA20_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDQ6292500.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="QBA20_001953"
     CDS             complement(7360..7599)
                     /locus_tag="QBA20_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDQ6292501.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /gene="nemR"
                     /locus_tag="QBA20_001954"
     CDS             7702..8301
                     /gene="nemR"
                     /locus_tag="QBA20_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDQ6292502.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="QBA20_001955"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="QBA20_001955"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDQ6292503.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="QBA20_001956"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="QBA20_001956"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDQ6292504.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="QBA20_001957"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="QBA20_001957"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDQ6292505.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..15224
                     /locus_tag="QBA20_001958"
                     /pseudo
     CDS             10766..15224
                     /locus_tag="QBA20_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15268..16476)
                     /locus_tag="QBA20_001959"
     CDS             complement(15268..16476)
                     /locus_tag="QBA20_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6292506.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16484..16984
                     /gene="tnpA"
                     /locus_tag="QBA20_001960"
     CDS             16484..16984
                     /gene="tnpA"
                     /locus_tag="QBA20_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDQ6292507.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="QBA20_001961"
     CDS             complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="QBA20_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDQ6292508.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17705..18532
                     /gene="mepH"
                     /locus_tag="QBA20_001962"
     CDS             17705..18532
                     /gene="mepH"
                     /locus_tag="QBA20_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDQ6292509.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18660..19241
                     /gene="sodB"
                     /locus_tag="QBA20_001963"
     CDS             18660..19241
                     /gene="sodB"
                     /locus_tag="QBA20_001963"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDQ6292510.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19387..20556)
                     /locus_tag="QBA20_001964"
     CDS             complement(19387..20556)
                     /locus_tag="QBA20_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6292511.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20722..20811)
                     /gene="ynhF"
                     /locus_tag="QBA20_001965"
     CDS             complement(20722..20811)
                     /gene="ynhF"
                     /locus_tag="QBA20_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDQ6292512.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            21110..22135
                     /gene="purR"
                     /locus_tag="QBA20_001966"
     CDS             21110..22135
                     /gene="purR"
                     /locus_tag="QBA20_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDQ6292513.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22132..23064)
                     /locus_tag="QBA20_001967"
     CDS             complement(22132..23064)
                     /locus_tag="QBA20_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6292514.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            23177..24388
                     /locus_tag="QBA20_001968"
     CDS             23177..24388
                     /locus_tag="QBA20_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDQ6292515.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24679..25827
                     /gene="cfa"
                     /locus_tag="QBA20_001969"
     CDS             24679..25827
                     /gene="cfa"
                     /locus_tag="QBA20_001969"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDQ6292516.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="QBA20_001970"
     CDS             complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="QBA20_001970"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDQ6292517.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26723..28096
                     /gene="mdtK"
                     /locus_tag="QBA20_001971"
     CDS             26723..28096
                     /gene="mdtK"
                     /locus_tag="QBA20_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDQ6292518.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIVHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28137..29393)
                     /locus_tag="QBA20_001972"
     CDS             complement(28137..29393)
                     /locus_tag="QBA20_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDQ6292519.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29701..29777
                     /locus_tag="QBA20_001973"
     tRNA            29701..29777
                     /locus_tag="QBA20_001973"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29735..29737,aa:Val,seq:gac)
     gene            29782..29858
                     /locus_tag="QBA20_001974"
     tRNA            29782..29858
                     /locus_tag="QBA20_001974"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29816..29818,aa:Val,seq:gac)
     gene            29966..30271
                     /locus_tag="QBA20_001975"
     CDS             29966..30271
                     /locus_tag="QBA20_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDQ6292520.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30397..32001
                     /locus_tag="QBA20_001976"
     CDS             30397..32001
                     /locus_tag="QBA20_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDQ6292521.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(32013..32825)
                     /locus_tag="QBA20_001977"
     CDS             complement(32013..32825)
                     /locus_tag="QBA20_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292522.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32829..33614)
                     /locus_tag="QBA20_001978"
     CDS             complement(32829..33614)
                     /locus_tag="QBA20_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDQ6292523.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33611..34279)
                     /locus_tag="QBA20_001979"
     CDS             complement(33611..34279)
                     /locus_tag="QBA20_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDQ6292524.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34343..34981)
                     /locus_tag="QBA20_001980"
     CDS             complement(34343..34981)
                     /locus_tag="QBA20_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDQ6292525.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34994..37095)
                     /locus_tag="QBA20_001981"
                     /pseudo
     CDS             complement(34994..37095)
                     /locus_tag="QBA20_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(37116..37742)
                     /locus_tag="QBA20_001982"
     CDS             complement(37116..37742)
                     /locus_tag="QBA20_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDQ6292526.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(38198..38407)
                     /gene="fumD"
                     /locus_tag="QBA20_001983"
     CDS             complement(38198..38407)
                     /gene="fumD"
                     /locus_tag="QBA20_001983"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HDQ6292527.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9259 a        10037 c         9411 g         9729 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccggct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgcaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tagcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccgac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctgggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcac gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccgcaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acagcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacggcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgtttgaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatggc gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctggcctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    15361 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    15421 tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg
    15481 ctggcttgtg tacgctggcg gaacagcaag cacctgaccg ccactccaga gctgcttgta
    15541 ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
    15601 ttttgcccgg acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt
    15661 gctgaccttc aaatcctcaa tgactatcat tgcgtggttt ttgctgacgg tcgttgtgac
    15721 tttgtgaagg tagtccctgc ggatatttgc gatacaggaa tgcagtcgct gtattttgcg
    15781 tttctgcttc tgccagttgt tgctgaattt gaccttccgg cttaactgtc gctgaagtct
    15841 cgccagcgtc ttctggtttt tttgaaaact gtttacaggc tcaaagactg tgccatctga
    15901 cagcgtggcg agtttagcca cgccagcatc cagcccgatc attgatgctg aagggtgaac
    15961 cggagttgat acttcacttt ctgtctgaat actgatgtac cacgtaccgc aggactggct
    16021 gacagtgaca tttttcacaa ctcccgtgac ctgacggcta ttccggtagc gcatccagcc
    16081 aagtttcggc agaaaaatac ggctgttttc ctgatcgagc ttaacgtcct gcgggtagcg
    16141 gaatgcagca ttctgtcccc gctttttgaa tcggggaaaa gcagcccgct tctggaagaa
    16201 gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc aatggctgtg agggcgaatc
    16261 tttaagccat tgcgtttcag tggcgttttt ccactcaacc agccaggaag ccattttccc
    16321 gtaagggatg tatttattac cggcctcatg attctcattc tgaagcgcca gagcacgatt
    16381 gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccaccggg
    16441 tcttaactgg aatttaaatg cctgtaatcg tttcacaggt caaatgatac tttggtctgt
    16501 gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct
    16561 ggtctttgtc accaaatacc gacgccagat ttttgaccat gacacgacag aaaaactacg
    16621 cacttacttt tcaaatgtat gtgctgattt tgcagctgaa ctggttgaaa tggatggcga
    16681 accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat ccagtctggt
    16741 aaacagcctc aaaggcatat cgaataggtt actgcgacga gatcgaccag atattgcagt
    16801 caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcgaagg
    16861 tgcgccaata tccgtcatcc gccagtacat tgaacaacag caaacacctg gtcaggtgga
    16921 aaaccgcgcc ttatatcccc gccctgaagg acgaggtttt acgacgcacc ggataaagga
    16981 ctgacggatg cccgttcgca tccgtcagta ttgcaggacg gattattccg cgtccggctc
    17041 ttcagacttg tatttagcgg cagtttcttt gatcagctgc tgcagttcgc cacgctgata
    17101 catttcgatc acgatatcac aaccgccgac cagctcgccg tcaacccaca gttgcgggaa
    17161 ggtcggccag ttagcatatt tcggcagttc cgcacgaatg tccggattct gcagaatatc
    17221 aacataggca aaacgttcgc cacatgcggc aagcgcctgg actgcctggg cagagaaacc
    17281 gcagctcggc agtttcggtg aacctttcat gtacagcagg atcgggtttt cagcaatctg
    17341 gcgttggatt ttttcgatag tggtgctcat tgtcttgctt cctcaaactt ctttacggca
    17401 gtaatctgac attgtagcgg gtcagtgcgg catcggaaaa taacattttc atcacgcttt
    17461 tgctatttta tccctttgct ctatttgttg catttcaaat attcgtttct tctctgatgc
    17521 gcggcaaact ggcgtattta taaccatttt tagctttcac tgctgcattt ttttgcgctc
    17581 gccaacgaaa cgtatttttt aacaataaaa gctattaact ttctcttctt ctatgcatta
    17641 gaatcatcaa gttttgtaaa tcagacgcag gcatgataga cctgccttaa cagagggacg
    17701 ctcagtggcg cggataaacc gtatttcgat cacgctctgt gctttacttt ttaccaccct
    17761 gcctttaacg cctatggccc atgcttcaaa gcaagccagg gagagttctg ctaccactca
    17821 tatcaccaaa aaagcagata aaaagaaaag cacggcaacc accaaaaaga cccagaaagc
    17881 cagccagaaa acagcgaaaa aagccgccag taaaagtatg accaaaagca aaaccgcttc
    17941 ttctgttaaa aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc
    18001 cgtcaataaa acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa
    18061 atcgcattgt gtgaaagtca aaaatgccgc gtcaggaacc cttgccgacg cgcacaaagc
    18121 gaaggtgcaa aaagccacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc
    18181 atatcgttgg ggcggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta
    18241 cgcttataaa gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct
    18301 gcgtgatgca ggacctatcg aacgtagtga gctgaaaaac ggcgacctgg tctttttccg
    18361 tactcaggga cgcggtacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat
    18421 tcagtcaccg cgcacaggtc aggaaattca aatcacttct ctcagtgaag actactggca
    18481 gcgccactat gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac
    18541 cctgttgtta cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct
    18601 atccatacgc acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa
    18661 tgtcattcga attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg
    18721 cggaaactat cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca
    18781 acctgattaa aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg
    18841 aaggtggcgt attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc
    18901 tggcaccgaa cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt
    18961 ttggcagctt tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt
    19021 ctggctggac ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta
    19081 acgcgggtac tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac
    19141 acgcttatta catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc
    19201 tggtgaactg ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag
    19261 cattgcctga tgcgcttcgc ttaccatgcc aacgcggatg aggcgttaac agcgcattcg
    19321 gcaagataaa tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt
    19381 gtttaattag ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac
    19441 cagcaataat gccagtcccg cgacaatcgc ccccgtcacc ggcacaaagc tgtatcccag
    19501 cccagcggaa attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc
    19561 accaatattg actgatgaag acagacctgg cgcttcactg gcgacatgca tcacgcgcat
    19621 ctgtaacgga ggtacaaccg caaaggttgc tgcgccccac accaccatgc taatagctgc
    19681 gccgaactca ttgcgggcca ggaacgggat tgccagcata atcaccatca gcagcaacaa
    19741 aaagcctttc aacgtgccgt taactgaacg atctgccagt ttgccgccga gatagttacc
    19801 gatagagaat ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg
    19861 ggtaatactt tgcagtaccg gagagatata ggtgtagagg gtaaacattg caccagctcc
    19921 cagtaccgtc gtcagcaatg cagacagcac ctgcggacgc attaataccg ccagctcttt
    19981 tttcacttca ggtcgtgccc ctgtaccacc tttaggtaat gagaagaaca gacttaccat
    20041 tgaaatcact cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc
    20101 caaccaggtc gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt
    20161 ggcaactgcg ctggcctgtt tatgttttgg caccacgctt gcggccacga ctgaacccaa
    20221 accaaaaaat gctccgtgat tcaggctggt caaaatacgt gaaagcatca gggtcatata
    20281 atccggcgcg atggcggaaa gaacgttgcc tagcgtgaaa attgccatca ggaaaatcaa
    20341 cgcactgcgg cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc ccaccattac
    20401 gccaactgca taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg
    20461 cgcaatgacg ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc
    20521 aatcgccagc gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc
    20581 attacgcgat attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata
    20641 acaaaaagag aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga
    20701 acgcgctccc tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ctacgatcaa
    20761 accgaggacg ataatcgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc
    20821 tcctttttta ttaccacaca aaaagtgata ttacgcattt ttacacactg tgatgaaaaa
    20881 atctcccgtc atttataatg ataagtgttt ttaccacttc cccttttcgt caagatcggc
    20941 caaaattcca cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg
    21001 tcgtttttcc ggcgtaccgc aacacttttg ttgtgcgtaa ggtgtgtaaa ggcaaacgtt
    21061 taccttgcga ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat
    21121 aaaagatgta gcgaaacgag caaacgtttc cactacaact gtgtcacacg tgatcaacaa
    21181 aacacgtttc gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca
    21241 ctactcccct agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt
    21301 gctggcgacc agcagcgaag cggcctattt tgccgagatc attgaagcag ttgaaaaaaa
    21361 ttgcttccag aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca
    21421 gcgggcttat ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc
    21481 tgagtaccca gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt
    21541 gatggactgg ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga
    21601 aggcggctac atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat
    21661 ccccggcccg ctggaacgta acaccggcgc aggccgcctt gccggtttta tgaaggcgat
    21721 ggaagaagcg atgatcaagg tgccggaaag ctggattgtg cagggtgact ttgaacctga
    21781 atccggttat cgcgccatgc agcaaatcct gtcgcagccg catcgcccta ctgccgtctt
    21841 ctgtggtggc gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggcctgcg
    21901 cgtcccgcag gatgtttcgc tgatcggtta tgataacgtg cgcaacgcgc gctattttac
    21961 gccggcgctg accacgatcc atcagccaaa agattcgctg ggtgaaacag cgttcaacat
    22021 gctgttggat cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg
    22081 tttgattgaa cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc
    22141 cgttgcggga gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgcc
    22201 gagatactca agcagccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca
    22261 acatgccgaa tccgggaagg ggttttccag ttcaagtgct acccacttcc cctcattaag
    22321 ccacggtttg gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt
    22381 tgccgatgat tcccagtcag gtacgacaag tcttttttgg ttatccagca accaggtaat
    22441 acgtttaggt agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt
    22501 atcatcgctg aacgggccat ccatcaacgc cagcgggtgg tgactggcaa caacgcaact
    22561 ccagcttagc atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt
    22621 tgcgccaatt gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac
    22681 ttcctgaaag acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg
    22741 ccgtgtacgt tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc
    22801 gttcgctatc tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc
    22861 ttctttgaga aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa
    22921 gagcggtacc gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac
    22981 acgatgcagc tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac
    23041 ttcgagtgaa tattctgacc acatagtctg cctgcaaaat ttttgaaacc agtcatcaaa
    23101 tattaccgtt tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga
    23161 gatttagaga atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag
    23221 cgtactcggt tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga
    23281 cctgcaaacg cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc
    23341 cgcagcccag cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt
    23401 aatcggcctg acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc
    23461 cacgctgctg gtattgcgtt ttgtacaggc tgtgggggtc tgcgccgcgg cggttatctg
    23521 gcaagcatta gtgacagatt attatccttc acagaaagtc aaccgtattt ttgcgaccat
    23581 catgccgctg gtgggtctgt ctccggcact ggctcccctg ttaggaagct ggctgctggt
    23641 ccatttttcc tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct
    23701 gcctattttc tggctcaaac ccacgacgaa ggcccgtaac aatagtcagg atggtctgac
    23761 ctttaccgac ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat acgcagcctg
    23821 ttcagccagt ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta
    23881 cagcccggca gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg
    23941 ttatggctgt cgcgccgcac tgcagaaatg gcaaggcaag cagttattac cgtggttgct
    24001 ggtgctgttt gctgtcagcg tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc
    24061 gctggtcgaa atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc
    24121 tattgttgtc gcccaggcgc tgcgtccctt cccacatgca accggtcgcg ccgcagcgtt
    24181 gcagaacact ctgcaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggcttat
    24241 tagtatcagc acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt
    24301 cgcgctgggt tacatgatgc aacgttgtga agaagttgac tgcccgaatc atggcaatgc
    24361 cgaagtcgct catagcgaat cacactgatc tatatcgata tacttatact taggctgcta
    24421 acaaaatttt gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct
    24481 acgataaata ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat
    24541 ttcggcaagg gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt
    24601 ctgcgagatt tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg
    24661 accagtgatg gagaaactat gagttcatcg tgtatagaag aagtcagtgt accggatgac
    24721 aactggtacc gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct
    24781 gccccggcgg atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaggaaggc
    24841 tctttggggt taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg
    24901 ttttttagca aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac
    24961 acgctgcgta ttgccggcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata
    25021 gtcggcaagg agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc
    25081 atgcaatatt cctgcgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg
    25141 aagctcaaaa tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt
    25201 ggctgcggct ggggcggact agcgcactac atggcatcta actatgacgt aagcgtggtc
    25261 ggcgtcacca tttctgccga acagcaaaaa atggctcagg aacgctgtaa aggcctggat
    25321 gtcaccattt tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct
    25381 gtggggatgt tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat
    25441 cgtaatttga aaccggaagg tatattcctg ctccatacta tcggttcgaa aaaaaccgat
    25501 ctgaatgttg atccctggat taataaatat atttttccga acggttgcct gccctctgta
    25561 cgccagattg ctcaatccag cgaaccccac tttgtgatgg aagactggca taacttcggt
    25621 gctgattacg atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa
    25681 attgcggata actacagtga acgctttaaa cggatgttta cctattatct gaatgcctgc
    25741 gcaggtgctt tccgcgcccg tgatattcag ctctggcagg tggtgttctc acgcggtgtt
    25801 gaaaacggcc ttcgagtcgc tcgctaaagg ctattctatc gccccctctt cgggggcgag
    25861 ttcagatcag gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg
    25921 ttctaccgta tctaccactg cctgagtttg tggatcgatt tcaatgttga cgcgtgcgcc
    25981 aagttttttc ttcccaagag tcgtgcgttc cagtgtttcc ggaattaaat ggacgcaaaa
    26041 acgcgttggc gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt
    26101 gtacaggata tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt
    26161 ttccgaggtt aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg
    26221 tccgccaatt tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt
    26281 taaatcgcca agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt
    26341 gccgttaatt tccgtcacgg tcaggcagca accgttatgc gccacggaag caccggtttc
    26401 caggccgtcc agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc
    26461 gtcaatcgac accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc
    26521 tgaaatcagt taagacattc tgttcagcac aatagcaggt ggaaaacgcc cttaccagtg
    26581 aaggggtaag aatggctatt ttttcactgg agaattaata aatcctcgct acaatagact
    26641 gaatttcccc tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata
    26701 actacaaata aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc
    26761 attagcaatc ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac
    26821 cgtgatggcg ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat
    26881 ctggcttccg gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc
    26941 gcaattaaat ggttccggtc gacgtgagcg cattgtgcat caggtgcgac aaggtttctg
    27001 gctggcaggt tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat
    27061 ccgctacatg gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc
    27121 gttgttgtgg ggcgcgccgg gatatctgtt cttccaggtt gcccgtaacc aatgtgaagg
    27181 tctggcaaaa accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacatccc
    27241 ggtgaactat atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg
    27301 tggcgtggct actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa
    27361 acgcgcccgc tccatgcgcg atattcgtaa cgaaaaaggc accgctaagc ccgagcctgc
    27421 ggttatgaaa cgactgattc aactcggttt gccgattgcg ctggcactgt tctttgaagt
    27481 gacactgttt gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg
    27541 acaccagatt gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc
    27601 agcggtaact atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac
    27661 cgctgcgcgg accgggctta tggtgggtgt ctgtatggca accctgacgg ccattttcac
    27721 ggtttcactg cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct
    27781 ggctgcgcat ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat
    27841 tggcagtggg attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac
    27901 agcttactgg gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt
    27961 tgaacctatg gggccagcag gcttctggat aggctttatt atcggcctga catcggcagc
    28021 cattatgatg atgctgcgta tgcggttcct gcaacgtatg ccgtcagcca tcattctgca
    28081 acgagcgtcc cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac
    28141 gcaggctgga gcgttgcgcc aatcccgtct tcgtctggct gtaatttcag agcgttacac
    28201 agagtttgcc gactggcatc tgttatctca acgcgtatat ccccgttaac ttcccctttc
    28261 acattgatga agtcaggtga aatttctttc gcactggttt gcaggcaaat cgctccctct
    28321 ccgcgcaggc gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt
    28381 tttccgccat tcagattgat gttaccaaca atgaaagtga cgctgtcacc ttttaccgta
    28441 ctgccgtcga gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagctct
    28501 gcgccataac caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca
    28561 accggtgcgc gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg
    28621 atcactgttc caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccgc
    28681 ccccccacat tcaccgacag gttttgcgtc ttaccggaac tgtagagctg aaggccatcc
    28741 gtccccagac gataaatgga tccattctca acaatagtgt cagagtcttc accaccgaga
    28801 ttattcatgg tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg
    28861 ccgccctgat taatctcaca attgctggct tctccacttg gatacaccaa caattcacca
    28921 ccttcatcta ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg
    28981 agaatgattt tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc
    29041 aacaaaccgc aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta
    29101 accgtagcga gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca
    29161 atggcaccgt cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc
    29221 ccaccggttg ccactttcaa ccagccagcc cccgtgacca cggtattttc ggctcttccg
    29281 ccagcttcaa caatcaactc gccttcagta agttgcgtcg cgccaaggac ctcgccggtc
    29341 ttaacaattt gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat
    29401 tcctttgctg catctgtgtg cctttattgc tacctaagtg taaaggctac ggaggattta
    29461 tccacgacag atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat
    29521 tgggttgtat cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa
    29581 tatagccaat ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt
    29641 gcctcttgcc acctttccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt
    29701 gcgttcatag ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag
    29761 tccaattgaa cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat
    29821 ggtgggggtc ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtctttt
    29881 cctttttccc tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt
    29941 atgcgttatc agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa
    30001 tggcccattt ggcgacgcaa tggctgagca gcttaaacca cttgctgagt cgattaatca
    30061 ggaacctggt tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg
    30121 gatctacctt ttcactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg
    30181 actgaaaaat ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact
    30241 tagtcaaatc aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc
    30301 ttttcatccc tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa
    30361 cactaaattc gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt
    30421 gccgggccta cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct
    30481 atttctatct tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa
    30541 aactcaaaaa tgatgctggc aaatattgcc agtattgaaa taccgccgat taattgtacg
    30601 tatctcgaat ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa
    30661 gaaaccttgc acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat
    30721 caatttttac gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa
    30781 tcatgccagg ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag
    30841 gatttaccca gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat
    30901 gaagaagaag caaccagaac gtattttccc agcccgtggt caggcctgat ggaagcaaag
    30961 gtcgatgcgt gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg
    31021 gcagtggcta ttcagcatgg ttcgttcatt gaagatgata aacaacacgt cgtttttcac
    31081 cgcgataacg caagtgaaaa gctaaatatt acgttgatgt cgcgcacggg tattttaccc
    31141 gaagccgatt tctattgccc tattccctac gagcccttac acatcgtcac cgatcaggca
    31201 ttaaatgctg agattcaaaa aggtgaagag ggccttttgg atcgggtatt tagattgata
    31261 gtagaggaaa tcaagtttgc tgatccagac tggagccaac gcatagcctt agagagcctg
    31321 aatgtcgatt cctttgctca agcctggttt gccgagcgca aacaacgcga cccatttgac
    31381 tgggcagaaa aaaatctcca ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc
    31441 tggcgttatg tcattctgcg cctgcatgaa gccgtacagg aaattgttcc tcatctgaat
    31501 gaacacgacc ataaacggtt cagtaaaggc cttgccaggg ttttcatcga taattatgcg
    31561 gcaatccctt cagagtctat tcgtcgacta ctggccttac gtgaagcggg gatcattcat
    31621 attctcgccc tcggtgaaga ctacgaaatg gaaattaatg agtcgcgcac cgtcctgaaa
    31681 acggaagaca acagctactc gtttgacgtt tttattgatg cccgcggaca gcgtccgctt
    31741 aaagtgaaag atattccttt ccctgggcta cgcgagcaat tacagaaaac aggggatgaa
    31801 atccctgatg ttggcgaaga ttatacgtta cagcaacccg aagatattcg tggacgcgta
    31861 gcgttcggcg cgttgccctg gttgatgcac gaccagcctt tcgttcaggg acttacggca
    31921 tgtgcagaaa ttggtgaggc gatggctcgg gcggtcgtaa agcctgcatc ccgtgctcgt
    31981 cggcgtcttt cgtttgatta aaggtaaagc tatcagcact caacaaactg atgctgttca
    32041 ttaagacgat agcggactcc ggttcgaata ttgttttcac cttcaatggc gacggcgaag
    32101 cgaactcttt cgccgtcatg atatgccagt gcggcgcttc cccctagccc gagaataatt
    32161 gagtcgacca cgcccgtgct cactaccgtt gaattttcac cgctggcaat gatgtgtaca
    32221 ttgtcgccgc tgttggcaat tctggcgttt gcgccaaagc tggcgatttg caccaggtca
    32281 ccattactgg cgacatgaca ccgttcaccc aatgtacaaa cgcgtactcg cattccggta
    32341 ttcgcaatcc gactactatc tccagcgctg cttatccgcg aagaattacc ggttacagca
    32401 acccgtgcgc gttcaccgga gctgccaatg tgactgttaa agccgacact tccaatccgc
    32461 acgctataac ccatactggc aatttgcgcg gcatacccag cggttgcgat tcgggcgtta
    32521 tcctcacaac agccgatgcg agcatgatcg tcgctgcggg caaactgact gcaaaatggt
    32581 atctctccgg ggtctgtggc cagtttttgc atagaggaaa cttcctgatg cgcgaagttt
    32641 tcatccgcca gccatttttt ccacgcatac tcaaccagac tctccgccca gtccgtatat
    32701 ccttcctcaa tcagtgcatg acgaatatcg gcataactac cgccgcgtgg gaaatggcgc
    32761 aggaaccagc gatacatcac cgctccgata cgccattctc gcaggtctgc tcgtgtaatg
    32821 atcattcgtt agtgccggtg atagccatcg accatgcttt taaaggtttc gtgtggcgta
    32881 cgccccgtgg tgcaaagata aagatgaccg aagataaaaa agaggcttat aaatgccaga
    32941 gcaaaatgcg cctgcaataa ccagtatctt acgccaggaa acacatctcc cacggcttgc
    33001 ggatagagac acaacagccc cgtcaatagt aacaacggca gcaatccata catgacacca
    33061 acataggcga cctgctgtaa gggattaaat ttagactgtg ttgttgccgg gaaaggatgt
    33121 tcttccccct gcataatgcc aaacaaataa aatcgcgttt gttttgccgc tcgttccagc
    33181 cacccctgac gacgaatgcg atagtggtga ccattatccc caacggcatt gatcagcaca
    33241 aagccgagcc agcatgccag taacaaaaat ccgcaaactt catgcaccgc aaccagactt
    33301 ttaaccgcag ttgcgcccac catcgcaaag tgatttatca gcccacttgc cagcaacaat
    33361 acaaagagta acgcattcga ccagtgccat aatctgaccg ctttgctgta aagataaacc
    33421 ttctcaccat gatcggtggc tgatttcttc acgccacgag cacgaagcaa ggcgtgcagc
    33481 cccagcacca accacatccc aaccagcaat actccggcaa tgatcaacca caccggccaa
    33541 aattcgggag tgaactgtgg cacatagttc gctaactggc tctgaaactg ttcagcatgt
    33601 tgcgacgggt tcatacattt tcctttttaa tcaaatgttg accgaaccgg cgatacagat
    33661 gcggttttcc ggcaccaggt agctgatatt gatagtattt attatcctgc aaccacgcct
    33721 gaatttctgg gctatcttcg cgaccaaaga tcaatgcgtg ttccggacag gcgctgacgc
    33781 aaatgggcgg aaagcctttt gccagtcggg actcagcgca gaaatcacat ttatctgcca
    33841 ctttagtgac agggttaagg tagcgaacct ggtacggaca agcgccaatg caataactgc
    33901 agccgatgca ctgcgatttt tctacccgca cgatcccctg ctcatcacgc cacgatgccc
    33961 ccgtcgggca tacgtcgatg cacggagcat cttcgcagtg ctgacatgac tggcgaaaaa
    34021 agtgatactg cgtttcgttg tcgttatcgg tgactggaat gtgtgctatc gataaacggc
    34081 ttccctgagc ggggacgtgg ttagttttac ggcatgcgcg ggcgcaaata ttacagccat
    34141 tgcaccgtga ctcgtcatga atcatggcat accgaacttc cttttcttgc ctcgtgttcg
    34201 ctaacagaga agacgcagaa ccagtaaaaa agattactgt tcccatcccc agaacaaatt
    34261 tgcgtcgagt gaaggacatc tgattatccc ctaatgcagt ggctgcaact gtccacccta
    34321 tccatgacat ctccctgaaa tttcaggggt tctgaaactg ctctacctcg aaccattcgc
    34381 ataagccata agccttgcca taggtcataa atcgctcgct aaacaagtac aacaccgccg
    34441 tcgatgactc cacctgccgt atatcggcat attcaggtgc aacatccatc gcggcgatcg
    34501 cggcttcaat ctgcgcttcc tggaagtaat atggcgcttg catcagcatg gcgactttgt
    34561 aaggacgagg atacgtctgg cactcaaaac gaacggcgtg tgcaatggcg cgacaaatgt
    34621 cctgctccac aacttgcaaa gacatcgccg cgtagttttc actcatggcc tgcgttgagt
    34681 aatagtaatc atcctgactt cccgtcatac aagcgatatc cgcgtagtct ccgttctgct
    34741 taatgctttc cagcaaaact gacagttcct cttctgcaac cgaatagggt ggcgagagaa
    34801 acacggtgcg cgccaccagc tgcccagccg cagaatgctg gcgaatgaac tcagccagga
    34861 tttcagcagg ttctggttcg gttaccgtat caacggggtg acgcatcccc tgcaaccatt
    34921 gccgcttcgc ctcgtcaact tcgctgactt tcgctaacgg caattcatcc tgatggttca
    34981 tcttacccat tccttacgca ggcagtagat tgtgtgctgc cagatcggcg gcaatatctt
    35041 ccagccccag acgctgcaat gtttcgcggg ttggacaacc aagctgtggg tcccagccca
    35101 tttctttgta gaacatcgtg agcgaagcat gcatatcgtc acgatccatt ttgtcagtac
    35161 cttcagtaaa gaccgggatc tgcggatcct tgtcgaaaac ccaggaacag ataagatcgt
    35221 gttcattacg catatctttg gtttgcatcg gttttaccgt gtaggcacga tgcaacgtaa
    35281 agatacgctc tgcagctaaa tctaattttt cctgagtcat ctcttcgccg gtgatcgctt
    35341 tgaagaattt ggcttcaagc gccagatcgc cccggtaatt acggcttttc agcggcgaaa
    35401 cggtcattgg ccagacccag ttgcacagag tgacggcgtt atgcaaacag acccgcaaca
    35461 gcgaccactt tgcatattta atttttgcgt cattgattgg cgtgtagttt ttggtttcat
    35521 cgtaagcatc ttcagaacca aaaagttctt tcgccacttc acgttgcagt ttcaatggca
    35581 agccggaacc aataaagttg atatgggtat gcgtcatgca gtcacggttg aacatacagt
    35641 taacaatgga accgacctgc gctgacgctt cattggcatg gtgaaccgga tagccaaacg
    35701 gcgaccagag tttatttttc gcgtagcccc agtactcttt acccaaattc cagcgttctg
    35761 cgatggcata tgaaccatca gccaggtgac tcagctcacc cacacgatgc gccagacggt
    35821 agtaaaaatc tttaatgaag ttaacgtcac ccgcttccag ttgatcccag cgaatttctg
    35881 catactcttc agctggcaga acacgcttga acacaccttt gctgtaacaa taagtaaagt
    35941 cgcgatgcaa ctgcccgtag ttacaccata ggccgtagtc atcgaacaaa ttcagaccaa
    36001 ccaggttacc aatcacacga ccatcgtctt tatcttcaaa atctttcggc ccgttcggga
    36061 agatggtggt atggacaaag tttgctacac aagtgttacc acctgtgctg ggcacgccaa
    36121 actctttcac ccgaggaata ttcatttggg tcatacaacg gatcgggcaa gagtggcagc
    36181 cgctcatttt tactgtgtat ttctctgccg ccggtcctaa gtcaaaaacg gatttatagg
    36241 tacgaaagcc gaccgtattc tgattgcctg gcggaatttc acccgtttca atcggaccac
    36301 cttcagccgc gccccaaaac agccctttac gtgctgtcca gcgtgacttg ggatctgaat
    36361 actctgccca cgattgtggc gtacttggca cgacatggtt gttattcgca ccaataagtt
    36421 cagtcatcat gtaatcattg agacgcttca tctcctgacg atcggcaatg ttgacccctt
    36481 tcgtcccttc aaccgcaatc gctttcaggt ttttcgaacc cattattgcg ccagttcccg
    36541 caccgccgct gtggttacgg ctattcaaca tgccagagag aggaataagg ttttccccag
    36601 cctgaccaat agccgccaca caggtttccg ggctggtcaa tcgacaaatt tcttccgtcg
    36661 ttgcgcgcgt cccttttccc cataagaaat cggctttttc caggctaact ttgtcatctt
    36721 taatcttcag ccataccggt gatttcgcct tcccttcgat aataatgacg tcgtatccag
    36781 cgaatttcat ttgcgctgca aaaaagccac ccatatgggc atcgcgacta aatttccttt
    36841 ggtaaaagta gaaagtgagg tgatatttac gcgagaacta cagggggcac cagatccagt
    36901 taatgggccg gtagcaaaga ctaatttatt cgcttcatcg aaaggtttcg tgcctagcgg
    36961 tacttcgtca tacataattt tgtagccgaa gcccatgcca ccgacaaaac ttttaaactt
    37021 actggaatct tcgagggtaa tatttcctgt cgtgagattg actcttaata tattacctgt
    37081 ccaaccgtta gccatgattt tttcctttgc aagattcaca cagtaatatc tttccactcg
    37141 ataattttta acgcccctgt tgggcaggcg tttgcgcatt caccgcataa cacacatttc
    37201 gaggattttt tactttcggt atttacggtg gccatcatcc acgggcatgc cgtagtacag
    37261 gcgctacagc caatacaacg tttatgatcg acggtaatac agccttcttt ctgctgccag
    37321 gtaatcgcac caatcgggca gacgttcatg cattgcggct ctttgcattg acggcaggtg
    37381 tccgcggtat agttgagatc gccatacagg ccgccgccag agccaacccc gttgtcgcca
    37441 aagaaataat tgcgatggat tttgatacgg gagaagaatg tccctactga gccatcgttg
    37501 aagttggtac aagagatttc acagcggtgg caacccgtgc atcgcgctcg ctgggtcacc
    37561 agcacccctt tgggtgtatt tatcaaccct actgtgccgc tatcaatatc ttcctgtttg
    37621 caacccaaaa gtgacagtaa cgcaggggca attgttagac cagcaaggcc ttttcctgat
    37681 atacgcaaga attctaatcg cgttaagcca atatctaata gtggacgatc aaccgggttc
    37741 atttatttcg catcctcttc gcaaacacag tgctgacaat tcagcagcct gatattaagc
    37801 attcaactaa tacgctgctg actctgtccc caacaccaga tattttccct aacccgatag
    37861 tgtatgagaa aagatttaac gccgataaac taatccttta tggttaggag aaaaaataat
    37921 gtgattattc tgagccctta acattgatcg ttatcaatta aaattacaaa gcaagaaatt
    37981 ataagcgaac tgatatttat tatcatttga aataaattta acttaagcga caatcatttg
    38041 tgacgttcat tgcagaaaat aaaaataaca ttatcgctat aaattaaaat acataccgaa
    38101 attcgtataa tttcagcagt tgttatttag aatgattaac tggaccgcaa ctgaagcaag
    38161 aatatcagga tgaagcttac cgcctcatcc tgaatggtca ttttaccagt gcgttaatga
    38221 cggtttccat cgcctgcttt tccagctcac tgcgctgaat gcgtttattg gctaatgctg
    38281 tacgtaatac gcctgcgata acaatatgct ttggttcctg ccccagatcg cgcatttcca
    38341 gcacaacttt accgacaact ctgcacattt ctcggtacag ttcatcctct tttgtccgat
    38401 ttcccattct ttatacctat ttatcatgcc aactat
//
LOCUS       DAOFMX010000035        37628 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.035, whole genome shotgun sequence.
ACCESSION   DAOFMX010000035 DAOFMX010000000
VERSION     DAOFMX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.035"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..176)
                     /locus_tag="QBA20_001984"
     CDS             complement(<1..176)
                     /locus_tag="QBA20_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDQ6292528.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="QBA20_001985"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="QBA20_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDQ6292529.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="QBA20_001986"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="QBA20_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDQ6292530.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="QBA20_001987"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="QBA20_001987"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDQ6292531.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="QBA20_001988"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="QBA20_001988"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDQ6292532.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="QBA20_001989"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="QBA20_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDQ6292533.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="QBA20_001990"
     CDS             complement(5180..5422)
                     /locus_tag="QBA20_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDQ6292534.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAHLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="QBA20_001991"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="QBA20_001991"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDQ6292535.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="QBA20_001992"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="QBA20_001992"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDQ6292536.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="QBA20_001993"
     CDS             complement(8339..9313)
                     /locus_tag="QBA20_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDQ6292537.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="QBA20_001994"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="QBA20_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDQ6292538.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="QBA20_001995"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="QBA20_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDQ6292539.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="QBA20_001996"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="QBA20_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDQ6292540.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="QBA20_001997"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="QBA20_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDQ6292541.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="QBA20_001998"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="QBA20_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDQ6292542.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="QBA20_001999"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="QBA20_001999"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDQ6292543.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="QBA20_002000"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="QBA20_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDQ6292544.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMYGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="QBA20_002001"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="QBA20_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDQ6292545.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="QBA20_002002"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="QBA20_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDQ6292546.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="QBA20_002003"
     CDS             23180..23383
                     /locus_tag="QBA20_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDQ6292547.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="QBA20_002004"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="QBA20_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDQ6292548.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="QBA20_002005"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="QBA20_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDQ6292549.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="QBA20_002006"
     CDS             26388..26660
                     /locus_tag="QBA20_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDQ6292550.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="QBA20_002007"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="QBA20_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDQ6292551.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="QBA20_002008"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="QBA20_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDQ6292552.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="QBA20_002009"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="QBA20_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDQ6292553.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="QBA20_002010"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="QBA20_002010"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDQ6292554.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="QBA20_002011"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="QBA20_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /locus_tag="QBA20_002012"
     CDS             complement(31907..32305)
                     /locus_tag="QBA20_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDQ6292555.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="QBA20_002013"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="QBA20_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDQ6292556.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33845..34273
                     /gene="rplM"
                     /locus_tag="QBA20_002014"
     CDS             33845..34273
                     /gene="rplM"
                     /locus_tag="QBA20_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDQ6292557.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34289..34681
                     /gene="rpsI"
                     /locus_tag="QBA20_002015"
     CDS             34289..34681
                     /gene="rpsI"
                     /locus_tag="QBA20_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDQ6292558.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35076..35714
                     /gene="sspA"
                     /locus_tag="QBA20_002016"
     CDS             35076..35714
                     /gene="sspA"
                     /locus_tag="QBA20_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDQ6292559.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35720..36217
                     /gene="sspB"
                     /locus_tag="QBA20_002017"
     CDS             35720..36217
                     /gene="sspB"
                     /locus_tag="QBA20_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDQ6292560.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="QBA20_002018"
     CDS             complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="QBA20_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDQ6292561.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9114 a         9418 c         9897 g         9199 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaaccggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatccg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagcgt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag tgagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgcgcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctcc ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatgtacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacttggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctagttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ccggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag ggcaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctgggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacggcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttcccaaaa
    33721 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    33781 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    33841 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    33901 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    33961 gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    34021 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    34081 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    34141 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    34201 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    34261 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    34321 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    34381 ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc
    34441 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    34501 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    34561 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    34621 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    34681 attggcttct gctacggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    34741 atggctaaat tctgaatcag cgtaaaaaat gggaagttgc tttttgctgc cgtctgaaag
    34801 acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag
    34861 cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa
    34921 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    34981 acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct ggtggctggt
    35041 agcagtaaaa attctgacta tacctagagg ttttcatggc tgtcgctgcc aacaaacgtt
    35101 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35161 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35221 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35281 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35341 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35401 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35461 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35521 tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35581 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35641 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35701 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35761 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35821 gacgcttcct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35881 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    35941 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36001 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36061 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36121 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36181 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36241 gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata ttcaccacga
    36301 aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa
    36361 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    36421 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    36481 gaataagact ggcaaatgac ataaacggcg cattgccaga gcccatcaca atggcacaaa
    36541 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    36601 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    36661 ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca aaaaacgcct
    36721 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    36781 ccgtcaaact tagtaacatc accaccacca gatgcagttc tgacttcatc agcccgacgt
    36841 gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa atggcgtaat
    36901 agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg atatttttat
    36961 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    37021 aagaggcgac cggaagctgg tagtggaaga agtatgtggc aattttcatt cccgcgacct
    37081 gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc gtggttgcga
    37141 taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga aataaggtga
    37201 ccatcagcaa catacccaga ccggaggcgc tggtaataaa ctgcgccatg atttggccga
    37261 tgacgtaagt tgccgacaga ataatatacg gcgagcgaat gagttttaac gggcggctta
    37321 gcaagctcac catcgcgcaa ctggctccta tgcgctccat gtagcgggca taaccgccca
    37381 ccgccataat cgacagcccc aatccggcaa tgcggttgga cagcatgcgc aggatttcat
    37441 tataaatgtc gacgatcaag tagccgctgc ttttggcggg atcgagtaca ccaccgaacc
    37501 ctaaccagac tccgcacatc atcaggaaga cacctgcggc agccagcacc acctgaggtt
    37561 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    37621 cgaacatt
//
LOCUS       DAOFMX010000036        36751 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.036, whole genome shotgun sequence.
ACCESSION   DAOFMX010000036 DAOFMX010000000
VERSION     DAOFMX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.036"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..246
                     /locus_tag="QBA20_002019"
     CDS             <1..246
                     /locus_tag="QBA20_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDQ6292562.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="QBA20_002020"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="QBA20_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDQ6292563.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="QBA20_002021"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="QBA20_002021"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDQ6292564.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="QBA20_002022"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="QBA20_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDQ6292565.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /locus_tag="QBA20_002023"
     CDS             complement(3257..3541)
                     /locus_tag="QBA20_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292566.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="QBA20_002024"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="QBA20_002024"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDQ6292567.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5570)
                     /locus_tag="QBA20_002025"
                     /pseudo
     CDS             complement(4442..5570)
                     /locus_tag="QBA20_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            5654..6190
                     /gene="rcdA"
                     /locus_tag="QBA20_002026"
     CDS             5654..6190
                     /gene="rcdA"
                     /locus_tag="QBA20_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDQ6292568.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6365..8050)
                     /locus_tag="QBA20_002027"
     CDS             complement(6365..8050)
                     /locus_tag="QBA20_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDQ6292569.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8320..8697
                     /locus_tag="QBA20_002028"
     CDS             8320..8697
                     /locus_tag="QBA20_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDQ6292570.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8727..8984)
                     /gene="grxA"
                     /locus_tag="QBA20_002029"
     CDS             complement(8727..8984)
                     /gene="grxA"
                     /locus_tag="QBA20_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDQ6292571.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9144..9431
                     /locus_tag="QBA20_002030"
     CDS             9144..9431
                     /locus_tag="QBA20_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDQ6292572.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9415..10137
                     /gene="nfsA"
                     /locus_tag="QBA20_002031"
     CDS             9415..10137
                     /gene="nfsA"
                     /locus_tag="QBA20_002031"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDQ6292573.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDFLHKQGWATR"
     gene            10198..11100
                     /gene="rimK"
                     /locus_tag="QBA20_002032"
     CDS             10198..11100
                     /gene="rimK"
                     /locus_tag="QBA20_002032"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDQ6292574.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11188..11664
                     /locus_tag="QBA20_002033"
     CDS             11188..11664
                     /locus_tag="QBA20_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDQ6292575.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12016..13128
                     /gene="potF"
                     /locus_tag="QBA20_002034"
     CDS             12016..13128
                     /gene="potF"
                     /locus_tag="QBA20_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDQ6292576.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13223..14356
                     /gene="potG"
                     /locus_tag="QBA20_002035"
     CDS             13223..14356
                     /gene="potG"
                     /locus_tag="QBA20_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDQ6292577.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14366..15319
                     /gene="potH"
                     /locus_tag="QBA20_002036"
     CDS             14366..15319
                     /gene="potH"
                     /locus_tag="QBA20_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDQ6292578.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15316..16161
                     /gene="potI"
                     /locus_tag="QBA20_002037"
     CDS             15316..16161
                     /gene="potI"
                     /locus_tag="QBA20_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDQ6292579.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16221..16709
                     /locus_tag="QBA20_002038"
     CDS             16221..16709
                     /locus_tag="QBA20_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDQ6292580.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16750..17877
                     /gene="rlmC"
                     /locus_tag="QBA20_002039"
     CDS             16750..17877
                     /gene="rlmC"
                     /locus_tag="QBA20_002039"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDQ6292581.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18052..18783)
                     /gene="artJ"
                     /locus_tag="QBA20_002040"
     CDS             complement(18052..18783)
                     /gene="artJ"
                     /locus_tag="QBA20_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDQ6292582.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19075..19743)
                     /gene="artM"
                     /locus_tag="QBA20_002041"
     CDS             complement(19075..19743)
                     /gene="artM"
                     /locus_tag="QBA20_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDQ6292583.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19743..20459)
                     /gene="artQ"
                     /locus_tag="QBA20_002042"
     CDS             complement(19743..20459)
                     /gene="artQ"
                     /locus_tag="QBA20_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDQ6292584.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20466..21197)
                     /gene="artJ"
                     /locus_tag="QBA20_002043"
     CDS             complement(20466..21197)
                     /gene="artJ"
                     /locus_tag="QBA20_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6292585.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21215..21943)
                     /gene="artP"
                     /locus_tag="QBA20_002044"
     CDS             complement(21215..21943)
                     /gene="artP"
                     /locus_tag="QBA20_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDQ6292586.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22160..22675)
                     /locus_tag="QBA20_002045"
     CDS             complement(22160..22675)
                     /locus_tag="QBA20_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ6292587.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23324..25093
                     /locus_tag="QBA20_002046"
     CDS             23324..25093
                     /locus_tag="QBA20_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDQ6292588.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25304..25627
                     /locus_tag="QBA20_002047"
     CDS             25304..25627
                     /locus_tag="QBA20_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDQ6292589.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25624..26454
                     /gene="amiD"
                     /locus_tag="QBA20_002048"
     CDS             25624..26454
                     /gene="amiD"
                     /locus_tag="QBA20_002048"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDQ6292590.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26451..27464)
                     /locus_tag="QBA20_002049"
     CDS             complement(26451..27464)
                     /locus_tag="QBA20_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDQ6292591.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27563..28993)
                     /locus_tag="QBA20_002050"
     CDS             complement(27563..28993)
                     /locus_tag="QBA20_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDQ6292592.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29004..30005)
                     /gene="ltaE"
                     /locus_tag="QBA20_002051"
     CDS             complement(29004..30005)
                     /gene="ltaE"
                     /locus_tag="QBA20_002051"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDQ6292593.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30042..31760)
                     /gene="poxB"
                     /locus_tag="QBA20_002052"
     CDS             complement(30042..31760)
                     /gene="poxB"
                     /locus_tag="QBA20_002052"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDQ6292594.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31893..32861)
                     /gene="hcr"
                     /locus_tag="QBA20_002053"
     CDS             complement(31893..32861)
                     /gene="hcr"
                     /locus_tag="QBA20_002053"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDQ6292595.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32873..34525)
                     /gene="hcp"
                     /locus_tag="QBA20_002054"
                     /gene_synonym="priS"
     CDS             complement(32873..34525)
                     /gene="hcp"
                     /locus_tag="QBA20_002054"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDQ6292596.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34669..35568)
                     /gene="lysO"
                     /locus_tag="QBA20_002055"
     CDS             complement(34669..35568)
                     /gene="lysO"
                     /locus_tag="QBA20_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDQ6292597.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36025..36720)
                     /gene="aqpZ"
                     /locus_tag="QBA20_002056"
     CDS             complement(36025..36720)
                     /gene="aqpZ"
                     /locus_tag="QBA20_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDQ6292598.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSAVVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9189 a         9135 c         9423 g         9004 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccaag cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 tttttgcccg taccgtcagg gattgcctga atggcgatat aaataggcgc gatacctacc
     5041 agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa aggccgtcag tgccatcccg
     5101 acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt gcatcatcgg caaaaccgtt
     5161 ttattcattt ctcgctcaac ggcggcacct tcaacgttta tcgccacttc cgcagaacca
     5221 aaacttgccc caaagacgcc gagaccaacg gcaaagagca ggggcgatgt cagccagagt
     5281 gccagactta atatcatcat cccgatcaat gcgcaggaca tcgtgaccag gatgacatta
     5341 cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa taccgctcat cgaaccgatc
     5401 gacagaccaa agagaacacc gcccatttca gcgatcgaga cagagagaat atcgcggata
     5461 gcaggcgtac gggttgccca ggacgccatt aacaggcctg gcaaaaagaa gaacataaac
     5521 agcgcccagg ttcggcgttt caatgcatta cgtgaagaat ttacggtcat agatcacgtc
     5581 aaaataagaa gagggaagac aacattagca aggttgtgta catttgtaca caattgcaga
     5641 cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg cgagaaaaaa ttatccaggc
     5701 cacgctggag gcggtgaaac tttacggaat acatgctgtt acgcaccgca aaattgctac
     5761 ccttgccggg gtaccgttgg gatcgatgac ctactatttt tcaggaattg atgagttgtt
     5821 gctggaggcg ttcagcagtt ttactgagat tatgtcccgg caatatcagg cattttttag
     5881 cgatgttagt gatgctcaag gcgcatgcca ggctatcacc gatatgatct acagctcaca
     5941 ggttgcaacg ccggataata tggagctgat gtaccagctc tacgcgctgg ctagccgaaa
     6001 accgctatta aaaacggtaa tgcaaaactg gatgcagcgc agtcagcaaa cgctcgaaca
     6061 atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt attgagggga tgacgctgca
     6121 ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt ttgaggatgg ttgagagggt
     6181 tgcagggtag tagataaatt ttagataaca aaaaacccat caaccttgaa ccgaaatggc
     6241 ggggttgatg ggctccacaa aatggggaca tcaaagaaaa gcagtggcac taattaagac
     6301 tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat ttcattttca gggcaacttc
     6361 agttttatcc taatcctggc cataccatga cgatgattgt ccctgccagc gtcagcagga
     6421 cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg gatgttactg cgagctgtat
     6481 cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc catcattgcg ccgaacaaca
     6541 gtgcgcggtt cattcgcaat acataagcac cgaacaagaa acagataacc acgggcacaa
     6601 gactgacaat taatccggca atcaacatct gaccgccaat cgcgcccagg ccgttattaa
     6661 taccgctacc ggcgctcaga ccaacgcctg ccataaacac catcaagccg aactctttca
     6721 ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg gtggttagca cgcataaagc
     6781 ccagcataat tccggcgaat aacaacccgg cagcgttccc catgccgaaa ctgaatgtgc
     6841 tgaactggaa ggtgatcatc ccgatcatca gcccaataac aaagaaggcg caaaatgcca
     6901 gcaggtcagt gacctggctg tgaatcgaga taaagccgat gcgatcggcg atggttttta
     6961 cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt gttaagcacg acgttgtcat
     7021 ctatcggcat ctcaatctgg ctacgaatga cgcggttaag gaagcaaccg tgatcggtca
     7081 acttcagttg tgcgagacgt ttacctacag cgttatggtt tttaacgacc acttcttcag
     7141 tgacgatacg catgtcgaga aggtcacgat cgaaaacttc tttaccgtta cggaagctgg
     7201 gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc tatttcatcg cccatttgta
     7261 gcacggcatc accgtctgga tttgccagaa tcccgttacg tcgaatacgt tcaatgtagc
     7321 agccggtttg tcgataaata cccagttcac gcagattttt gccgtcggtc caggccacca
     7381 gctccgggcc gacgcgatag gcgcggatca ccggtaaata aaccttacgg ttggcatcag
     7441 tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt ctgtaagtcc tgatgctgca
     7501 atttcggcaa gtaacgcgca ccaacaatca aactcaccag accgattaaa taggttaagg
     7561 catacccgag gctcagatta tccagtgcca gtgagagctg cctgctttcc atgccggaat
     7621 gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga cgtcatagag cctgctaaca
     7681 taccggccgt caggccaata tcccagccaa acagcttacc taaccctaag gcgatcacca
     7741 gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt tttcccatca cgaaaaaaaa
     7801 tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa aataaacagc ataaagccaa
     7861 gattaagcgc atcggtgtta atgctgaaat gttgttggcc taataacagc gatacgacta
     7921 aaacgccaat ggaattaccc agttggatcg aaccaagtcg taactttccg agacatagcc
     7981 caagcgcgag gaccacaaat aataacagaa tgtaattccc atttaacaat tcggcgacgt
     8041 ttatattcac ggaggctaac ttcttgttta ctagtaagct gttgaaagaa atggtaattt
     8101 acgataatgt gttttaccag aattcagggc gcagattcat tcagcgcacc taaacgatag
     8161 taaagtaaca atatatttta ctagtgtaat cacattaggt atcaacggct atatgaattg
     8221 cgttggccta tattagcatg gaatgcgaag cggctttatc ttactgaacg ccacactggc
     8281 gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag tgaaacataa acaacgttgg
     8341 gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt gcctttttct ggcgacgcac
     8401 atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg gcttgctgtt tttcattctt
     8461 cctggagcag ttgccagttt cttttcacag cgtagagaag tcctgaaacc tctatttggc
     8521 gcaatgctgg cggcaccctg ttcaatgctc attatgcggc tgtttttttc accgacgcgc
     8581 tcattctggc aagagctggc atggttacta agcgcggtgt tctggtgtgc gctgggggca
     8641 ctgtgtttct tatttatcag tagtttgttt aaaccacagc acagaaaaaa tcagtaaagc
     8701 cctcaacgcg agggcttgtc agacgatcag gcgtccagat tttctttcac ccatgcagca
     8761 aaatcggtat agccgccgat atgttgctga tcgacaaaaa tctgcggcac ggtttctacg
     8821 ggtttacctg ccttttgttg tagatcttct ttagtgatcc cttccgcacg aatatctaca
     8881 tactgatact gaaaatcatc gcgttcattg ctcaatttct cagccagatc ttttgcacgc
     8941 acacagtaag ggcaacccga acgaccaaaa ataacggttt gcattatttc tctcctcata
     9001 gatttatgcc tgtaatgatc acgctaaaat gtattcgctg aaagtaggtt taacctgttg
     9061 cattaattgc taaaagctat aactgttaaa cacaatacag tgaaaagttt tagactgaag
     9121 gctcactttg cagagggaag cgtatgcgcg cgatcggtaa attgcctaaa ggcgtgttga
     9181 tactggaatt tatcggaatg atgctactgg cggtggcgct gctgtcggta agcgactccc
     9241 tgtcgctgcc tgagccattt tctcggccag aagtgcagat tctgatgatt tttctcggtg
     9301 ttttgctcat gcttcccgct gcggtggtgg ttattcttca ggtggcaaaa cgtcttgccc
     9361 cacagctgat gaaccgtcca ccgcaatatt cacgttcaga aagagaaaaa gataatgacg
     9421 ccaaccattg aacttatttg tggccatcgc tccattcgcc atttcactga tgaacccatt
     9481 tccgaagcgc agtgtgaggc gattattaac agcgcccgtg cgacgtccag ttccagtttt
     9541 ttgcagtgta gtagcattat tcgcattacc gacaaagcat tacgtgaaga actggtgacg
     9601 ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt tctgggtgtt ctgtgccgac
     9661 tttaaccgcc atttacagat ctgtccggat gctcagctcg gcctggcgga acaactgttg
     9721 ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat taaccgcagc ggaatcgctg
     9781 ggattgggcg gggtatatat cggcggcctg cgcaataata ttgaagcggt gacgaaactg
     9841 ctgaaattac cgcagcatgt tctgccgctg tttgggctgt gccttggctg gcctgcggat
     9901 aatccggatc tcaagccgcg tttaccggcc tccattttgg tgcatgaaaa cagctatcaa
     9961 ccgctggata aagacgcact ggcgcagtat gacgagcaac tggcggaata ttacctcacc
    10021 cgtggcagca ataatcgctg ggatacctgg agcgatcata tccgccgaac aatcattaaa
    10081 gaaagccgcc catttattct ggattttttg cacaaacagg gttgggcgac gcgctaaaac
    10141 cgctacgtcg atgtatgata cgcgggcttt tgaccaggtc tgacagagag gtgcagggtg
    10201 aaaattgcca tattgtcccg ggatggaacg ctctattcgt gtaagcggct gcgtgaagcc
    10261 gctatacagc gcggtcacct ggttgaaatt cttgatccgc tttcttgcta catgaacata
    10321 aatcctgcgg cgtcttctat tcactacaaa ggccgcaagt taccccattt tgacgcagtg
    10381 atcccgcgta ttggcaccgc cattaccttt tatgggacgg cggcactgcg ccagttcgag
    10441 atgctgggga gctatccgct caatgagtcg gtcgccattg cccgggcgcg tgacaaattg
    10501 cgttccatgc aactgctggc gcgtcagggc atcgacctgc ctgtcacggg cattgcgcat
    10561 tcgccggatg ataccagcga tttaatcgac atggtcggtg gtgcgccgct ggtggtcaag
    10621 ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg agactcgtca ggcggcggaa
    10681 agcgtgattg acgctttccg cggtctgaac gcgcatattc tggtgcagga atatatcaaa
    10741 gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg atgaagtggt cgctgcgatt
    10801 gaacggcggg cgaaagaggg ctattttcgt tccaatttgc atcgtggcgg cgcggcaagc
    10861 gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa aagccgcgcg aacgatggcg
    10921 ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg ggccgttggt gatggaagtg
    10981 aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg gtatcgatat cgcgggtaaa
    11041 atgatccgct ggatcgaacg ccacgctacg acagaatatt gcctgaaaac gggtggttag
    11101 tcgcaatcac attactgatc atggttttgc ctgcgctttt tgcgtaagct gtgccggtct
    11161 ttttatcgaa agaggttgta caaaattatg acatcgctgg tcgttcctgg tctggatacg
    11221 ctgcgtcaat ggctcgatga cctggggatg agtttttttg aatgtgataa ctgtcaggct
    11281 ctgcatctgc cccatatgca gaatttcgac ggtgtctttg atgccaaaat cgatctgatc
    11341 gataacacga tcctgttttc tgccatggcg gaagtccgac cttcagccgt attgccgctg
    11401 gcggcggatt tatctgccat caatgccagt tcgctgaccg tgaaagcatt tcttgatatg
    11461 caggatgata atctgccaaa gctggtggtt tgccagtctt tatccgttat gcagggcgta
    11521 acctatgagc agtttgcatg gttcgtgcgt cagagcgaag agcagatttc gatggtcatt
    11581 cttgaagcta atgcccatca actgctgtta ccgactgacg atgaagggca aaacaacgtt
    11641 accgaaaact attttctcca ctgataattc ctttcgagca cgcagtcgct gccgcagtga
    11701 ctgcgcgctg caaaattatc tgctgttttt aaccttttct taaagattat ttcacttctc
    11761 ttgtgtcgat ttggctttat cacatagagc aaatacgcat aaaaatttgt taaataccgt
    11821 tttttaatcc gagctatagt ctcaaaccct ggctaaagtt attcttgcga tgcttctata
    11881 tagcgagcag tgctggccgg gagagagttc tcttttctta caccgcgccg ataaaaaaat
    11941 atgcacgttt attgcatatc tttcagtgtg acaacttttg ttcgtttgtt aacgaacttt
    12001 cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc tatcgggtct ggttgcgggt
    12061 gctctgatgg ccgtctctgt cggcacgctc gcggctgaac aaaaaacact ccacatttat
    12121 aactggtctg attatatcgc ccctgacacg gtggccaatt ttgaaaaaga aaccggtatt
    12181 aaagtcgtct acgatgtttt cgactctaac gaagtactgg aaggcaaatt aatggcgggt
    12241 agcaccggct ttgacttggt ggttccatct gccagctttc tagaacgcca gttgactgcg
    12301 ggagttttcc agccgctgga caaaagcaaa ttgccggagt ggaagaatct cgacccggag
    12361 ctgctgaagc tggtcgccaa acacgatccc gacaataaat ttgctatgcc ctatatgtgg
    12421 gcgacgaccg ggattggcta taacgttgat aaagttaaag ctgtgctggg cgaaaacgcg
    12481 cccgtcgata gctgggactt gatcctcaaa cctgaaaatc tggaaaaact gaaaagctgc
    12541 ggtgtctctt tcctggatgc gccagaagaa gtttttgcta ccgtgttgaa ttatctcggc
    12601 aaagatccca acagcactaa agcggatgat tacaccggac cggcaaccga tctgctgtta
    12661 aagctgcgcc cgaacattcg ttatttccat tcatctcaat acattaacga cctggcaaac
    12721 ggcgatattt gcgtcgctat cggctgggca ggtgatgtct ggcaggcgtc aaaccgcgcg
    12781 aaggaagcga agaatggagt gaatgtctcg ttctcgattc caaaagaagg ggcgatggcg
    12841 ttctttgatg tattcgccat gcctgcggat gccaaaaaca aagacgaagc ctatcagttc
    12901 ctgaattacc tgctgcgccc ggatgtagta gcgcatattt ccgaccatgt gttctatgtc
    12961 aacgccaata aagcagccac gccgctggtg agtgcggaag tccgtgagaa cccgggtatt
    13021 tatccgcctg cggatgttcg tgcgaagctg ttcactctga aagtgcagga cccgaaaatc
    13081 gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg gaaaataatc cgcagcctta
    13141 gatgccgggc gggcgcacca cacccgccgg caattcgcac catcatggtg cgcttgcaca
    13201 cattcaatgc cggagagcag ccgtgaatga cgctatccct cgcccgcagg cgaaaacccg
    13261 taaggcgctg acgccgctat tagaaatccg caacctcacc aaatcctacg atggtcaaca
    13321 tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa atcttcgcgc tgctgggcgc
    13381 atccggctgt ggcaagtcca cgctgctgcg tatgctggca ggtttcgaac aaccttctgc
    13441 cggacagata atgcttgatg gcgtcgattt gtcacaggtt ccgccttacc tgcgccccat
    13501 caatatgatg tttcagtctt acgcgctgtt tccgcatatg accgtggaac agaacatcgc
    13561 ttttggcctg aaacaggaca aactaccgaa agcggaaatt tccagtcggg tcaatgagat
    13621 gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa ccgcatcagc tttctggtgg
    13681 tcagcgacag cgtgtggccc tggcccgaag ccttgccaag cgcccgaaac tattactgct
    13741 cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac cggatgcagc ttgaagtggt
    13801 ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc acccacgatc aggaagaggc
    13861 gatgaccatg gcggggcgca tcgccattat gaatcgtggg aaatttgtcc agattggcga
    13921 accggaagag atctacgagc atccgactac ccgctatagc gccgaattta ttggctcggt
    13981 aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat ggcctggtgc ttgattcgcc
    14041 ggggctggtg catccactga aagtcgacgc ggatgcctcg gtggtcgata acgtgccggt
    14101 acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa gagccgcccg ccaatggttg
    14161 taacttcgcg gtgggggagg tgatacacat tgcctatctc ggcgatcttt cggtgtatca
    14221 cgttcgtctg aaaagtgggc agatgattag tgcccagcta caaaacgccc atcgtcatcg
    14281 taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc tgggaagtgg acagctgtgt
    14341 ggtgctgacg gtttaaggag caaagatgaa tacacttgaa cctgctgccc agtcgaaacc
    14401 gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg aagcatgggc gcaaactggt
    14461 cattgcgttg ccatatatct ggttgatctt gctgtttctg ctgccatttc tgattgtctt
    14521 taaaataagc ctggcggaga tggcgcgcgc tattccacct tataccgaac tgatggagtg
    14581 ggctgacggg caactgtcca tcactcttaa tctcggtaat tttctgcaac tgaccgacga
    14641 ttcgctctat ttcgatgctt atctccagtc gctacaggtg gctgcgattt cgacattttg
    14701 ctgtttactg atcggctatc cgctggcgtg ggcggtggcg cacagtaagc cttcgacccg
    14761 taatatttta ttactactgg tgatcctgcc gtcgtggacc tcgtttctga tccgcgttta
    14821 tgcttggatg ggaatattaa aaaacaacgg tgtgctgaat aattttctgc tgtggctggg
    14881 ggttatcgat caaccgctga ccattcttca taccaatctg gcagtttata ttggcattgt
    14941 ttacgcctac gtgccgttta tggtgctgcc gatttatacc gcgttgattc gtattgatta
    15001 ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg ctgaaaacgt tctttactgt
    15061 gatcgtgccg ctgactaaag gtgggattat tgccggatcg atgctggtgt ttatcccggc
    15121 tgtgggcgag tttgtgatcc cggaactgct cggtggcccg gacagcatca tgatcgggcg
    15181 cgtgctatgg caagagtttt tcaataaccg cgactggccg gtggcctcgg cggtagcgat
    15241 catcatgttg ctgctgctaa ttgtgccgat aatgtggttc cacaaacacc agcaaaaaag
    15301 cgtgggagaa cacggatgaa taatttaccg gtagttcgtt cgccctggcg gattgtgatt
    15361 ttgctgctgg gctttacctt tctctacgcg ccaatgctga tgctggttat ctattcgttt
    15421 aacagctcga agctggtgac ggtgtgggcc ggctggtcaa cgcgctggta tggtgagtta
    15481 ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc tgacaattgc tgcctgtgcg
    15541 gcaacggcgg cggcgatcct cgggactatt gcggcggtgg tgctggtgcg ctttggcagg
    15601 tttcgcggat caaatggctt tgcctttatg atcaccgcgc cgctggtaat gccagatgtc
    15661 atcacgggct tgtcgctgtt gttattattc gtcgcgcttg ctcatgccat tggctggcct
    15721 gcggaccgcg gtatgcttac catctggctg gcgcatgtca ctttctgtac ggcttatgtg
    15781 gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct cgatagaaga agccgcgatg
    15841 gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta cgctaccgat gatcatgccc
    15901 gcgatcattt ctggctggtt actggctttt actttgtcgc ttgatgatct ggtgatcgcc
    15961 agctttgttt ccgggccggg agccaccacg ttaccgatgc tggtcttttc cagcgtgcgg
    16021 atgggggtga atccggaaat caacgccctg gcaacattga tcctcggcgc ggtcggaatt
    16081 gtcggattta tcgcctggta tctgatggcg cgcgcagaaa aacagcggat acgcgacatc
    16141 caacgtgcaa gacgtggctg aagacactaa aatttgccaa cctggctaca taatgccgcg
    16201 catgtcgcgg cattgttttc atggaagacg aaacgttggg attttttaag aaaacatctt
    16261 catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt ggcggcgctc gccattatta
    16321 tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct gggcgtgcgc ggtattggcg
    16381 agttcattca tcgcagcgta caaacctgga gtttaacgct ggtcttttta agcagtctgg
    16441 tgctggtttt cattgagatc tggtgtgcgt tttcactggt gaaagggcgt cgctgggcgc
    16501 gctggctata tctgctgaca caaatcaccg ccgcaagtta cttgtgggcg gcttcgctgg
    16561 ggtacggtta tccggagctg ttcagcattc ccggtgaatc aaaacgtgaa atcttccata
    16621 gcctgatgct gcagaagctg ccggatatgc tcatcctgat gctgctgttc gttccctcga
    16681 ccagtcggcg gttcttccag ttgcaataat gtgtataatc gtcgcccctg atgatgtgaa
    16741 ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct gtcgttcctg tcagtggata
    16801 acgcagccga ttccagagca actctccgct aaaaccgccg atcttaaaaa tctgctcgcc
    16861 gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc cggaacaagg gtttcgtaat
    16921 aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac tgctcggtat gctgcatcgc
    16981 gatggcacgc cggaagacct ttgtgactgc ccgctttatc cagcctcatt tgcgcccgtt
    17041 tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa cgccctacaa cgtggcgcgt
    17101 aagcgtggcg aactgaaata cattctgctg actgaaagcc agagtgatgg cggcatgatg
    17161 ctgcgctttg tactgcgttc tgataccaag ctggcgcaac tgcgtaaggc gctgccgtgg
    17221 ttacacgaac aactaccgca gctgaaagtt attaccgtca atattcagcc ggtacatatg
    17281 gcgattatgg aaggggagac ggagatctac ctgaccgaac aacaggcact ggcggagcgt
    17341 tttaatgacg taccgctgtg gatccgtccg caaagtttct tccagactaa tccggcggtc
    17401 gccagccagt tgtacgccac cgcgcgcgac tgggtacgac agctgccggt taaacatatg
    17461 tgggatctgt tctgcggtgt ggggggcttt ggtttacact gcgcgacgcc tgacatgcag
    17521 ttaaccggga tcgaaattgc gccagaggcc attgcctgtg caaagcagtc agccgctgaa
    17581 ctgggcttaa ctcgtttaca atttcaggcg ctggactcca ctcagtttgc cactgctcag
    17641 ggggaggtgc cggagctggt gctggttaac ccgccgcgcc gcggcattgg taaaccgctg
    17701 tgtgattatc tttcaacgat ggcaccgcgt tttattatct actccagctg taacgcccaa
    17761 accatggcga aagatatccg cgaactgcca ggttaccgca tcgaacgggt acagcttttt
    17821 gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc tgctggtgaa gcaataaaaa
    17881 agccacaggt gcggcttcag attgctgatc aggtgcgctt tgttcatgcc ggatgcggcg
    17941 tgaacgcctt atccggccta caaaatcatg ctaaatcaat acattgcaga gttgatgtgg
    18001 gccggataag cgcagcgcat caggcaatct tacttttgtc atcagtctca attactgcgg
    18061 aaaccactgg tcattgattt tctgatatgt accgtcagct ttaattgctg ccagcgcgtt
    18121 attcagtttt tccagcagag ctttgttatc cggacgtaca gcgataccca ggccggtgcc
    18181 gaagtattgc ggatcggtca ctttttcagt cgcaacgccc agttgcggat tggttttcag
    18241 ccattcgttt accaccgcgg tgtcaccaaa taccccatca atacgaccat ttttcagatc
    18301 gataaaggca ttctgataac tgtcatagga gacagttttc acttccgggt gctgatcctg
    18361 aatatatttc tggtgcgtcg taccgttttc catcccaatg cgtttgcctt tcaggtcggc
    18421 aaacgttttg taggtatctt ttttggaaat cacgacggct gagttttcat agtagggtgt
    18481 ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc ataccggaga ttacggcgtc
    18541 atattttctg aatttcagtg acgggatcag gctgtcgaac gcgtgattag taaaagtaca
    18601 ttctgcctgc atttgtttgc acaaggcttt tgccagatcg atatcaaagc cgacaatctc
    18661 attattagca cctatagatt caaagggtgg ataggtggct gaaacgccaa aattgatttt
    18721 ctctgcggca gaagcaccga aagtaaagga agcaagtaaa gcggcaagaa ctaacttttt
    18781 catgatggaa ctcccgtctg tcaatcttat gatttttggc cgtgtctgcg gcatgggata
    18841 acaatgccat caagtgaatt tatatgcaat aaacatgatt aaataattta aatgaaataa
    18901 aaaaagacgg ataacttagt gagttgtccg tcttcattat aagaatttat gcactatgta
    18961 ggccggataa ggcgtccacg ccgcatccgg cacaggcacc gtgctgatgt ctgatgcgac
    19021 gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa tgtacgcagc tacattaatt
    19081 ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc atcatcagcg tcagcaggcc
    19141 gttgacgacc aggtaaataa tccctgccgc accgaacacc attacatcgt aggtgcgtcc
    19201 gtacaacaac tggctgtatc ccatcacttc catcagcgta atggtgtaag ccagagaggt
    19261 acttttgaac accagcacca cttcgttgga ataagaagag agcgagcgtt taaaggcata
    19321 cggcagcagg atcgccagcg tatctttttt gctcattccc agggcgctac aggactgcca
    19381 ctgaccttct gggatcgcac gaattgcacc gtaaaacagc tgcgtggtat acgccgcact
    19441 gttcagcgat aacgcaatca gcgcacataa ccacggttct gacaacaaat gccacagtgc
    19501 cggatactcc tgcaacgtcg gaaactggcc cggcccgtaa taaatcagga agatctgcac
    19561 cagcagcggc gtaccggtaa acagcgtgat ataaccccgc accagccaca ccagcaccgg
    19621 cgttttcagc gtcaggatga tggtaaaaat caatgccaga atcagtgcca caatcagcga
    19681 ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc agctcgggta aatactcaaa
    19741 cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt caatgcgttt gagaatgtac
    19801 tgactgagca gggtgatcac caggtaaatc gccgccgcca caatgtacca ggtaaatggt
    19861 tcctgggtac gagtagcaat gcttttggtt tgtaacatta aatcattcac actaatcaaa
    19921 ctgaccagcg cggtatcttt cagcagcacc agccactggt taccgaggcc aggcagcgca
    19981 tgacgccaca tctgcggcat caccagacgg aaaaagatag ccgatttcga cagccccagc
    20041 gcctgaccag attcccactg gcccaccggt acagctttca acgcgccgcg cagcgtttgc
    20101 gaggcatagg cggcatacag cagtgacaga gcgatgacac cacaaaggaa cggactcacg
    20161 tcgaagttct caatgtccat ctgcaccggg atctgcacga acccaagatt gatagtgaag
    20221 ccatccgaaa gcgtcagcag taactgcgag gagccaaaat agataaacag cactaccaga
    20281 atttctggca ggccacgcag aatggttacc agcgctgaac ctgcccacgc gacaggacgc
    20341 catttcgctg actcccatac cgcaaagaac atcgccagcg ccagcccgac aatcaatgca
    20401 caaacggcaa ggccgacggt catcccggcg gcgcttgcta aaggaaaaaa ttcattcatc
    20461 aggaattact tctggaacca tttgttgtag atggtttcgt aagtgccatc tttcttcact
    20521 ttttccagcg cagtgttgag tttctgctgc agttcagtgt tgccctgacg taccgcgatg
    20581 ccgaggccag taccgaaata atctttatcg gtcactttat cgcccactgc cgccagtttt
    20641 gggttatctt tcagccactc agtgaccact gcggtgtcac caaatacgct gtcgatacgc
    20701 ccgttttgca gatccagttt tgcgttctgg tagctgtcat acggaacggt ggtgatttcc
    20761 gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt tctgaacgcc gacttttttg
    20821 cctttcagct gatcaacact ggtgtatttg ccttgctgac ccacaaacag ggcagagttg
    20881 tcatagtacg gggtagtaaa cagtacctgc ttttcacgct ccggagtgat atccatgccc
    20941 gccatcactg cttctacgcg acggaatttc aggcttggga tcaggctgtc aaacgcctgg
    21001 ttagagaaag tacaggttgc atcaatctct ttacacagtg cctgtgccag gtcgacgtca
    21061 aaaccaacga tctggttgtt tgcatcaatc gattcaaacg gaggataggg ggcttcggta
    21121 gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa aacctgcaat taacgcggca
    21181 atcagaactt ttttcattgt tgttatcccg aatcttagtg agagagatag tttttaaatg
    21241 cttcggtttg cggctcggta aagcagctcg cgtcgccttg ttctacgata tgaccatttt
    21301 ccatatacac cactcggctg gcggttttac gcgccacttc aacttcgtgg gtgacgatca
    21361 cctgggtaat attcgtttct gccagctcac gaatgatgct gacgatttgt gccgtaattt
    21421 ccgggtccag tgcggcggtc ggttcatcga acagcagtac ctgcggttcc atcatcaacg
    21481 cacgggcaat agcaacacgc tgctgctgac caccagaaag atgcagcggg taacgatcgc
    21541 tataaggttt gagacgcaga cgttccagca gtttttctgc acgggccagc gcctgatctt
    21601 tactcaaccc cagtacacgg cagggcgctt caatcaggtt ttgctgcacg gtcagatgcg
    21661 gccacaggtt gtattgctga aacaccatgc caacgttacg acgcaaatcg cgaatcgctt
    21721 tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc aatgttgagc gtaccggagc
    21781 gcggcatctc aagcagattg agtacacgca gcagcgagct tttacccgcg ccgctggggc
    21841 caagtaacac cagcgtttcg ccctgtgggc aatccagcgt gatatcgaac agcgcctgat
    21901 gcgcgccgta gaagcaatta atgccgttta attgaatact cattgacact cgtatactgg
    21961 agtctgatag ctattgaggt cgaagatagt acctttgaca gaataattat gcaatattta
    22021 tgctttaaaa gttaaaagca aagcgcatta ttcaataaac atagcacaaa ataacggggg
    22081 cggtggtcgg tgagcataaa tgtcggcatt cctcacgaaa tgccggataa tttacggggt
    22141 ttattggttg atcaaggcgt tagcgattct cgatggactg acggagcgta cccgccgtgg
    22201 catgaacgct accgcctaag tagcgcacat cgtcgatgac ccaacactgg ccttcctgaa
    22261 tcattaacac ttcatcctgc cagccctggt caccctgttt gagatccacc cgcaatggaa
    22321 tgttacgggc atcacgatta gggatagtcg atgcactggc aacgtgggcg ctgtctggca
    22381 aggtggttcg actggagaac ggatcgctgc ttaacaattc acgatgacta ttatcgcggc
    22441 tggcatcgct aagttgggtt gccagtttgt cactcaaata ggggcgcagg gcggtaatgt
    22501 cattgctgcg atgttgaatg cggtagtcgt agaattgttg agccacactg tcaggccctc
    22561 cctggacaca ggaaccggtg cgggggccgt tatctttata agctggcgtg acggtggtac
    22621 aggcactcag cagcagtgca catggaataa gtagagatag tgaacggtag cgcataatca
    22681 tttccttatt agcttgctgt ctgcgtcaca ctacgaccaa acgctatcag attatttaat
    22741 acattgtttt taatacaatt ctgaaacaat ctgaacacct gattgggttt gagtccattg
    22801 ctggctaccc actcaaggtt gctcgtagct gacttactta aattacccaa acaatagaca
    22861 gtgtaaacca tttgtgaggt tgagcgtgtt atatggctga aatgatcatt taaggtaaat
    22921 atgatagttc tgaaatggtg gtataaatcc tcctgtagag gttaagagca tctgcaagaa
    22981 gccagtttca catttataac gcgtgataaa tgaatgcggt tattgtctaa tcattacaaa
    23041 atttttcaca tgcgataaga gatcctgcga ttaagatgat tgccaaaatt acgttgtatt
    23101 tttgaaaatg agcgaaattt gatagtccca gctttgactc tcgataatat tatttatgct
    23161 acaattgtgg gtaaagaatt taagctaatg aatttgagtg aagaataaat taaattgctt
    23221 cagtatttcg ttaattaata taaatacaaa aggcgttgtg cttttataat tataaagcgc
    23281 gaggagttcg gtggattatc gtttttcttg ggtgattttt aatatgataa acaatatatc
    23341 tgatcaagca tcatcatttc caggcacaca actaaatcaa agtgataatt tcctggattc
    23401 actgagggaa tttttcgcga tattaaattc cagtcgtaag ggtgagttgt ctacatggga
    23461 taccatctac ttgcatctga tattagcaat aaatgctgac agtgatctca taaaaaatga
    23521 tgtgttgctc gcggaaaata ttccatcagc gaattaccaa tttaatacat tttttagcaa
    23581 tactttcgag attgatgtta aaaaatatct gggtaaatca gaagataacg aagttgaaat
    23641 aaaagcgggt aatgaaagaa tctctattgg aatacgtaat gtatcgaatg gaaaacttga
    23701 aagacaacag tttcttttcc cactggacta tgagaataaa ttacaagaac aattagataa
    23761 gtattttacg attgaatcgc acccattatt atacaggtat accattggta gtaagattgc
    23821 gaatgtgatt tttgaaaagt tatacagtcg tattgacttt aacaaagaac aatatatatc
    23881 ctttataaag gacgcattta ttcattttta tgattatagc aggcgatatg ctattagtga
    23941 gaatatcgat aaagacgctg taacaaacaa tatcgcattg atgagcactt tttatgacag
    24001 tgataatacg tcaggtgagg ttttaaataa tgattttaca gaagaagagt catttgaaac
    24061 tgcgttagat gtagagcatg caatagtgct tggatttgct gatgataatt ttgaaacaaa
    24121 acctgttcat tatcaggatt tattaacacg atttagtgcc tttcaagata ccgtgtttaa
    24181 tctgtttcct gaaatgcact cttcgcatta ccacgatatt tgtagcgtaa gtgtggatat
    24241 gacaaaggga acgcaatgca tgattcactt gatggtgaat gaagaagttt tcatgtctct
    24301 gcctgttcca gttgccacga tggtacgtga agatgcaagt aatttagtca atcttaaaac
    24361 actactcaat gatggttgtt tcattaaata tagtcatttt aatgacgtgg cattaataaa
    24421 gcaaaacata agtaatctct atttatcaca cactgttgtg aatgaaagca tattgaaaaa
    24481 atgttgtttt gaaaacggca gtttaggtga tgttaaaata acaaatagca atgtaataaa
    24541 cagtgcattc aaaaatattt cgtttagatc tgttaaaatt aataacgtaa atactcattc
    24601 tttaaaattt attaactgca attttttcaa tgttgatatg ataagagtta atctcagcaa
    24661 atgtcttttt catgaatgta gtatgcatgg tgtcaagata aaaccctggt tgcctgtaaa
    24721 atggacaaaa gaattaataa gtgattattt gtatggctgt ttactatctc tgtactctat
    24781 ctgtgcaagg gatatatata atatgaacgc cgggaataac gtgaaggttg ctgcggatgc
    24841 tttccttgaa attattttct ctcttaaaaa taaatattgt attaaactat taagcgctca
    24901 ggatcgggcg tttatatacg agtttgcaag aatgatcttc gcttatatta atgacaaaag
    24961 cattgagatc ttattacttt cttgttttgc ggcggctgac caaaaagcaa tccagcggta
    25021 tagacctcag tcacaagatg gggaagactt taggagtcat ttacaatata agttgcctct
    25081 ttccgctcat taatgttgtt ggtgcaacat aataagacag gtaagcggta ccctataatg
    25141 aaaccaatat cgggttatgc aacggataat cgtaaagact taagtgggtg tttttgacac
    25201 aatgagatga tttcctaaac tgatcaaaac cttcttccca acatggcata tcagtccgat
    25261 aatgctttag agatcgaagg cttaacaaac aaggagatcg atcatgcaat tttctacaac
    25321 cccaactctg gaaggcctga ccatcgttga atattgcggt gtggtgaccg gcgaagcgat
    25381 tttaggtgcc aatattttcc gtgatttctt tgccggtatc cgcgatatcg ttggcggacg
    25441 ttccggtgcg tatgaaaaag aactgcgtaa agcacgggag atcgcctttg aggaattagg
    25501 ctcccaggcg cgggcgctgg gggccgatgc cgtagttggt attgatatcg actacgaaac
    25561 ggtcgggcaa aacggcagta tgctgatggt tagcgtcagc ggtacggcgg tgaaaacgcg
    25621 tcgatgagaa gagtcttctg gctggtcgct gccgctctgt tactggcagg gtgtgcaggc
    25681 gaaaaaggca ttgtcgagaa agagggatat cagcttgata cccgacacca ggcgcaggcg
    25741 gcgtatccgc gcattaaagt gctggtgatc cactacaccg cagatgattt tgatagctcg
    25801 ctggcgacac tgactgataa gcaggtcagc tcgcattatc tgatccctgc ggtgccaccg
    25861 cgatacaacg gtaaaccgcg catctggcaa ctggtgccgg aacaagaact ggcctggcat
    25921 gcggggatta gcgcctggcg cggggcaacg cgccttaacg acacctctat tggcattgag
    25981 ctggaaaacc gtggctggca aaaatcatcc ggagtgaaat attttgcccc gtttgaacag
    26041 gcacagattc aggcgcttat tccgctggcg aaagatatta ttgcccgtta tcacatcaag
    26101 ccggaaaacg tagtggcaca tgcggatatc gcaccgcagc gcaaagacga tccggggcca
    26161 ttatttccct ggcagcaact ggcgcagcag gggattggtg cctggccgga tgcgcagcgg
    26221 gtgaactttt accttgccgg gcgcgcgccg cacacaccgg tagataccgc gtcattgctg
    26281 gagcttttgg cgcgctacgg ttatgacgta aaacctgata tgacaccgcg cgagcagcgg
    26341 cgcgtgatta tggcattcca gatgcatttc cgcccgacgt tatataacgg cgaagcggat
    26401 gcagaaactc aggcgattgc cgaagcattg ctggagaaat acgggcagga ttagcgcggc
    26461 agttttccgt ggtcgcgtag ccaggcggca gttttctcga tgccttcatc cagggtgatg
    26521 accggctgat aacctaactc ttcctgcgca cgcgtaatat ccagcgtaaa gtcaaaattc
    26581 aacttggaga cgccgtagtg ggtcagcggc ggctcttttg ctgacttgcg gcctaaacgc
    26641 tccatgctgc gggcgatcat atccagcatc gggtaaggga cggaacgaat acgacagtca
    26701 atattcaact cgtcgatcag cttctgcacg atgctgcgca gtgtgcgatg ctcgccgttg
    26761 gtgatgttgt acacacgccc ggaaggtagc ttatcgcagg cttcctggct tgccagccac
    26821 attgcgtgca cggcattttc atagtaggtc atatcgacca gcgcactgcc gccatgcggt
    26881 aacagaatac tgccgtagtg gtgcatcata tgcgccagac ggggaataaa gactttatcg
    26941 tgcggtccga acagactttg tgggcgcaga atagtaaagc gcgtttgtgg attcgcctgc
    27001 gaaagcatat tgatcacttc ttcgctggct gctttgctgc gggcaaactc gttggcgaag
    27061 cggtgagggc gaaaatcttc tttaatattg cgatggtggt gataatcgaa gtacagggag
    27121 ggggaagaga tatgaataaa gttacgcaca ccccaggcga cagcccattc acccaggcgg
    27181 cgagtggcgc gaacgttagc cagatcgaaa gcctgttgtg tcccccaggg tgaggtaaag
    27241 ctggagcagt gccacagcgt atcaatgccc gcgagcatca ctttagcttg tgatgaaacc
    27301 agctcggtca gatccgtcgg aacaaactct gcgcccattt tttccagcaa tttacccatt
    27361 gcttcgttgc gaccgctcgc acgcacgctg atgcctttct ggcataaaaa ctctaccgcg
    27421 tttcgaccta agccgctggt ggcgccggta accagtacct tcatatcaat ccactgttgt
    27481 tgagaaaatg acgtgcgcat tcttccgtga tttcccccat gatgcaatgg gaaacatgaa
    27541 agaataacgc aggttttgtc gattaatctg tgctttgttc tgccagtctg gcgatttgtt
    27601 ttgccattcc gcgaaaaata aacagatgcg cggggatcat caataaccag taaaacagcc
    27661 ccggcatacc gtgcggatgc cagaaagcgc ggacatcgat agtacgatag tcacctttat
    27721 cttccagggt aaaacacagt cgtcccagcc ccggcgcttt catgccaaat aacaacgtaa
    27781 gttgcttttt cggttcaacg acaatcactt tccagctatc caccgcatcg ccggtctgta
    27841 aatattcgcg ctccgggcgg cctttcgcca gattatgacc gatcgcgcgg tccatcaacg
    27901 cccgtgtctg ccacaaaata ttgccaaaga aataacgctc tttaccgccg atttggttca
    27961 ccaccagcca taaagccgca aggctggcgg acgttttaac ggtaaacccc gcctgtttgg
    28021 cgaaataacc gtactccggt cgccagcggg caaaggcctg agcgtcgtag ccccagtcgc
    28081 tggagttgac cagtttttct tcctctttca acgtgctacg taccgcgtca tcgaaagcga
    28141 tcagccgttg tgggatgagt gcacgtagcg cggtatcatc tgccagcaga tcgtgtttca
    28201 gcccctgaat caacgccctg gcggtggtgg gcggtacgga agtaatcaca ttgagaaacc
    28261 acaccgaaat ccagcgggtg gggaggggga tggggatcaa ccagcggcgc ttaccgctca
    28321 ccgccataaa atgttcaaac tgttgctgat aactgagcac ctctggtccg gcggcttcga
    28381 agatgcggtg ttcgttggct ggatggtcta acagcgccac cagatagtgc agcaagtttt
    28441 ccagcgcgat gggcgtggtg cgtgaacgta cccagcgtgg cggcgttaac actggcaggt
    28501 tgtagaccat atcgcgcatg acttcgaacg ccgctgaacc tgcgccaacg ataattccgg
    28561 cccgaagttc ggtcactggt acattcgctt cacgaagaat gtccgccgta gcctgacgag
    28621 ctcgcagatg atccgactgc tcatgtggcg gggcctgcaa cgaactgaga aagattaatt
    28681 gcttaactgg tacttcacgt agcgcatcgc ggacgttgag agccagctgg cgctcctgag
    28741 cgataaaatc gccgccttcg cccatgctgt gcaccagaaa atagacggta tcgatatcct
    28801 gcaacagggc cggaaggtta tccggccagc tgagatcgac tttatggcaa ctgacgtttg
    28861 ccagttgcag ctttgcaagc ctgtcgacat gacgtgccgc cgctaggatc tgatgccctt
    28921 gctggctgag tgtgcgcacc agatgctgac caatgtagcc actggcaccg agaactaaaa
    28981 tgcgttgcgg cacgtatctc tccttaacgc gccaggaatg cgcgccagtg ggcggcgact
    29041 tccgccagtt gttcgcgcga gacgtcaaga tgcgtcacca ggcggacaat cggcgaggcg
    29101 ttaatcagca cgtttctcgc tttcatgtat tcgcctaacg cggcagcatt ttcttccccg
    29161 acgcgaacaa acagcatatt agtgtcctga cgcatcacat ccgcgcctgc ttcacgcagt
    29221 tgctccgcca tccaggcggc gttgtcgtga tcttcctgca accgtgcgac gttatttttc
    29281 agggcataca tcccggcggc agccagaatc ccggactgac gcatcccgcc accggccatt
    29341 ttccgccagc ggatggcacg tttaatgtaa tcacgattac cgacgagtaa tgaaccgact
    29401 ggcgtcccaa gacctttcga caggcaaatg gtgaacgaat cacaatattg cgtgatctct
    29461 ttcagttcgc agccgtaagc caccacggca ttaaagatgc gcgcaccgtc aacatgcagc
    29521 gccagattgc gctcgcgggt aaattcccat gcttctttca ggtattcacg cggcagcact
    29581 ttgccgttgt gggtgttttc cagactgagt aatttggtgc gggcgaaatg gatatcgtcg
    29641 ggtttgattt tcatcgccac tttatccagc ggtagcgtgc cgtcggcagc cgcgtcgatg
    29701 ggttgcggct gaatactgcc cagcaccgct gcgccaccgg cttcaaacag atagttatgc
    29761 gcggcctgac cgacaatata ctcttcgcca cgttcgcagt gactgagcag agcgaccagg
    29821 ttggcctgag tgccggtagg cagaaaaatg gcggcttctt taccggaaag ctctgctgca
    29881 tagtcctgca gagcattaac ggtagggtcg tctccgtaaa cgtcgtcccc aaccggggcg
    29941 gccatcatcg cttcgagcat ggcgcggctc ggtcgggtaa cggtatcact gcgtaaatca
    30001 atcatggcat gtccttatta tgacgggaaa tgccaccctt tttaccttag ccagttcgtt
    30061 ttcgccagtt cgatcacttc atcgccgcgt ccgctgataa ttgcgcgcaa catatacagg
    30121 ctaaaacctt tggcctgttc gagtttgatc tgcggtggaa tggctaattc ttctttggcg
    30181 accaccacat ccaccaacac cggaccgtcg atagagaagg cgcgttgcag ggcttcatca
    30241 acttcagagg ctttttctac acggataccc gtaatgccgc acgcttcggc aatgcgggca
    30301 aagttggtgt cgtgcagctc agtaccgtct gtcaggtaac ctccggcctt catctccatc
    30361 gccacaaagc ccagcacgct gttattaaag acgataattt tcaccggcag tttcatctgc
    30421 actactgaga ggaaatcgcc catcaacatg ctaaaaccgc catcgccgca catggcgacc
    30481 acctgacgtt ccggctcggt tgcctgcgca cccaacgcct gcggcatggc gttagccatc
    30541 gaaccgtggt taaacgaacc taacaggcga cgcttgccgt tcattttcag ataacgtgcc
    30601 gcccataccg ttggcgtgcc gacgtcacag gtaaagatgg cgtcatcggc ggcaaaatga
    30661 ctaatttgct gcgccagata ttgcgggtga atggctttct cgctcggttt agctaaatcg
    30721 tccagccctt tgcgggcatc gcggtaatct tccagcgctt tatccagaaa cttgcgatcg
    30781 gttttttctt ccaccagtgg cagcaatgcg cgcagggttg atttgatatc gccgaccagc
    30841 gccatatcta ccttgctatg cgcgccgatg ctggctggat taatatcaat ctgaataatt
    30901 ttggcatcgg tcgggtagaa cgcacggtag gggaactgag taccgagtag cactaacgtg
    30961 tcggcgttca tcatggtatg gaaacctgac gagaatccga ttaacccggt cattccaaca
    31021 tcatacggat tatcgtattc gacatgctct ttaccgcgta gggcatgaac aataggtgct
    31081 ttaattttcc cggcaaactc aactaactct ttatgcgccc ccgcgcagcc gctgccacac
    31141 atcagggcga tattgctgga ataacgcagc agttgcgcca gtttgcgtaa ctcttcttct
    31201 tccggcatca cgactggctg tggcgcatga taccagtgcg tggttgcccc ttctggcgca
    31261 ggttttaacg ccacgtcgcc cggtaataca acaacggaaa cgccacggtt aagcaccgct
    31321 ttgcgcatag caattgccag cacttgtggg atctgctccg ggctggaaac cagctcgcaa
    31381 tagtgactac attcgcggaa tagctcttgt gggtgggttt cctggaaata gccgctgcca
    31441 atttcgctgg agggaatatg agcggcaatc gccagtaccg gaacgttatt gcggtggcaa
    31501 tcgaataggc cgttgatcaa gtgcaggttg cctgggccgc acgatccggc acagaccgcc
    31561 agttctcctg aaagttgtgc ttcagcgcca gcggcaaacg ccgccacttc ttcatggcgg
    31621 gtggacatcc actcgatggt gcccatgcga ttaagactgt cgctaagacc gttcagagag
    31681 tcgcccgtga ctccccagat gcgtttcacg cctgccgatt cgagtgtttt ggcgatataa
    31741 gctgcaaccg tttgtttcat ggttctccat ctcctgaatg tgataacggt aacaagttta
    31801 gttcatctga cggaggggga agggatggga gagaaaggag gcactaacgg ttaaatagcc
    31861 cgatgaaagg aatatcatcg ggcataaggc gattatgcga gaaccaaatc cccctgcgga
    31921 tggcaggagc aggccagtac gtaaccttca gcgatttcgg cgtcggtcag cgtcattgta
    31981 ctgctcaccg tatattcacc ggaaaccact tttgtcttac agcagccgca aacacccgca
    32041 cggcaggcag cgacaaccgg aacgttattg ctttccagcg cctccagtag cgtggtgcca
    32101 accggggcgt aaaattctcg tgccggttgc agtttggtga atttcagacc gctggtcgcc
    32161 gcttctgcta ctggggtgaa gaatttctct ttaaagaaac gcgtcacgcc gagcgctttc
    32221 acttcctgct ctacccaatc catatacgga gccgggccgc aggtcatcac ggtacgtgaa
    32281 gctaagtcag gtacatctgc cagcagttcg cgagtgagac gaccagcgat aaagccttcg
    32341 gtaacgttat tttctgccac cagcgttacc ggatagttac gccactcatc ggcgaaaata
    32401 acatcctgcg gcgtacgcac gttgtagatc acccgcacat cggcctgtgg acggttcttc
    32461 gcaagccagc gacgcatcga cataatcggc gtgacgccgc agcctgccgc cagcaacagg
    32521 aatttatctt ctgctttatc gtcgcaggta aattccccca tcgcgtccga aagccagaga
    32581 taatcaccgc gttttacatc gcgcgtcagc cactgggagc cgacaccgtc atcaatccgc
    32641 cgcacggtca gggtgatata ttcactcacg cctggcgtgg aggaaatggt gtaagcacgc
    32701 agcgtttccg ctgagtttcg cacgctgacc agtgcatatt gcccggcgcg atatgggtag
    32761 tgatcgtggc aaatcaggga aatcgtccac acatccggcg tttcttgcgt aatgtgatga
    32821 acctgtatcc gccacgggca ttgattcgtt ggcatcgtca tcgacaaact ccttacgcgt
    32881 tcaacagttg cttcatgtct tcttcaacag tggtgataga acgcaggccg aatttctcgt
    32941 tcagcaccgc cagcaggtct ggtgtcagga aaccaggtgc agtcgggccg gtgacgatat
    33001 ttttcacgcc cagagaaagc agcgtcagca gaatgacaat cgctttctgt tcaaaccagg
    33061 agagcaccag cgacagcggc agatcgttca caccacagcc cagtttctct gccagagtga
    33121 cagccagaat aattgctgag taagcatcgt tacattgacc tgcatctacc agacgcggca
    33181 gaccttcgat atcgccaaac tcaagtttgt taaagcgata tttaccacag gcgagggtca
    33241 ggatcaggca gtcatccggc acgctggtgg cgaaatcggt gaagtagtgg cgctcgccgc
    33301 gtgcgccgtc acagccacca agcaggaaga tatgacgcag tttttcacgg ctaaccagat
    33361 caatcagcgt atcggctgca ccaagcagcg tctggcgacc aaagcccacg gtgatcaggt
    33421 gaggaatttc gctgtacggg aagcccgcca tttgttgcgc ctgggcgatg accgcagaga
    33481 aatcttcgcc atccagatga cgcacgccag gccagccaac gatgctgcgg gtccagatac
    33541 gatcgtcata agcgcctacg gttgggtcga tgatgcagtt cgaggtcatc acgatggggc
    33601 cagggaaacg agcgaactcc acttgctgat tctgccagcc gctgccgtag ttaccgacca
    33661 gatgcttgaa tttacgcagc tccgggtagc catgcgcagg cagcatttcg ccgtgggtgt
    33721 agacattaac gcccgtgcct tcggtctgtt ccagcaggtt gtagagatct ttgagatcgt
    33781 gaccggaaat cagaatgcat ttacccgccg tcgctttgac gttgacctgg gttggcgtcg
    33841 ggtgaccgta tttaccggtt tcgcctgcat ccagaatgct catcactttg aagttcatct
    33901 ggccgatttc cattgaacac tcaagaagcg cgttcatatc ggcaggccag gtccccagcc
    33961 acgccatgat tttatggtac tgggcataaa tatcgttgtc gtattgaccg agaacatgcg
    34021 cgtgttccat ataggccgcc gcacctttca ggccatacag gcacagcaga cgcaggccga
    34081 gaatgttttc gccaatcgcc gctttatctt tgttaggggt aaattctgct gcctgacgtt
    34141 gcagctcgcc gagatcatcg ctcaccagtt gcaggtcagc catcgggtta tcgacgcgcg
    34201 cgttggcatc tacagccagg cattgtgctt tcagcgcctc gcgcaaggca atcgcttcac
    34261 gagcgtagcc gacaatacgc ggagaatcga agttaacgtt ggtcagggtt gagaaaaagg
    34321 cacgtggcgc gaagctgtca acatcgtggt tgatgatgcc gtattcacgc gcttttaccg
    34381 cccaggcaga aagcccttgc agcgccgcga tgagtaaatc ctgaaggtca gaagtttccg
    34441 ccgttttacc acacatcccc tgcgcgtatg agcagccgtt ttctgccgga gtacggatag
    34501 tttgttcaca ttgcacacaa aacatgatca caccttttaa agttatattt aatatacatg
    34561 tttaagggta agacgcttaa cgcggggata aaagggattt ttcatgcaac tttaagggag
    34621 attgatttag cgcaattttg gcggcaggga actaccgcca gagaggtatt acgcagagaa
    34681 aaaggcgatg aggatcggca ctaacaggct aagaataaaa ccgtgaacaa ttgccgccgg
    34741 gaccatatcc agcccgccag tacgttgaag aacgggcagg gtgaaatcca ttgatgtggc
    34801 accgcataag cccagtgcag tagagcggct gcggcgaatc agcccaggga tcaacataat
    34861 agcaatcagt tcacgggcca gatcattaaa aaacgccgcg ctcccgatta ccggaccaaa
    34921 agattcggtc aataaaatac cggaaagaga ataccagccg aaaccggagg ccattgccag
    34981 cgcggtattg atggggagat caagaataaa ggcgttaatt aaaccaccaa ttaatgaact
    35041 gacaaccacc accacggcga caatcattcc ccggcgatta aggacaatct gctttaaggt
    35101 catgccatta ttgcgcaact gaataccaac gaggaaaagt agcaaaatta acgtgtattc
    35161 actggcttcg gtcgcgtgtt gtaagaaagc cagtccactc agaccaatgg caaaaccaat
    35221 cactactacg ccgcacagtt ttagcgactc cagcgccatc gcaatacgcg acgggagttt
    35281 ttcttgctga tggtggttgc gccacggcag gcctcgctcc agccacatca gggcggcaat
    35341 attacacagt aaaataacgg taatactgac ggcagaataa tgcagaatcg ccaacaggtt
    35401 actggcgagg ttatcgagaa acgccagact gatacccata aaaaagagaa taaggtaaac
    35461 catccagctt aatagctgat taatagcttt taacgcagct tgttggcgaa gcggaatgag
    35521 gtaacccaca atcaggggaa ccagaatgat taacagccca gaaaacatga aaatccagtc
    35581 cttgcaaaga agaagtcgaa atgcgcgatg acacactact gaaagcggaa gggcgagtaa
    35641 agttgcaatt aaaaggaaat gttatgcatc agagggggat ttttaatcct atctgagtgt
    35701 ttagcattgt tgcgctcctt tcatgtgcaa tgccatataa agccactggc ggagggagcg
    35761 cgcagggggc agccagtcgc cgccgccccc tgcacccccg ggctctggcg aacaaaatcg
    35821 ccgctgcgcg gtaccttcag cttatcccct tcggcaaccg ggtcgggcgc gaggtaacgt
    35881 cctgtaagac gcgccctcag cccacatcca tgtgggctgc cccggccttc ggggaacgct
    35941 tcagcgattt tgacgccacc aatacccgtg cggtttgtaa tgtcgaagat aaaacaacta
    36001 gggcagataa aatgccaatg ctaattaatc acgcttttcc agcagagtcc ggtaaatcag
    36061 accaccgata atgccgccga caattggcac cacccagaag aaccacagtt gttctaatgc
    36121 ccagccgccc tggaagatag caaccgcggt gctgcgcgcc gggttaacag aagtgttagt
    36181 caccggaata ctgattaagt gaatcagggt taaggccaga ccaatagcga tcggcgcaaa
    36241 acctgccgga gcgaatttgt cggttgcacc gtggatcact aacaggaagc ccgcactcaa
    36301 taccagttca actaccaccg cggaaagcat ggaataaccg cctggtgaat gctcgccata
    36361 accgttagaa gcaaaaccgc tggctgccgc gtcaaaaccc gttttaccac tggcaattaa
    36421 atacagcagc gccgctgcaa caataccgcc gacaacctgg gcaattacgt agccaacgac
    36481 ttcttttgcc ggaaaacgtc cgccagccca taaaccaata gtgaccgccg ggttaaaatg
    36541 accaccagaa atatgaccaa cagcgaaggc catcgtcaga acggtcaaac caaatgccag
    36601 cgccacgccg gcaaaaccaa tgcctaattc cgggaagcct gcggccagta cagcactacc
    36661 acagccacca aaaacaagcc agaaagtacc aaaacattca gctgctaatt ttctgaacat
    36721 atccacctca attaaaaatt gaccctgtga a
//
LOCUS       DAOFMX010000037        36520 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.037, whole genome shotgun sequence.
ACCESSION   DAOFMX010000037 DAOFMX010000000
VERSION     DAOFMX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.037"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(156..1391)
                     /locus_tag="QBA20_002057"
     CDS             complement(156..1391)
                     /locus_tag="QBA20_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDQ6292599.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="QBA20_002058"
     tRNA            complement(1538..1614)
                     /locus_tag="QBA20_002058"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="QBA20_002059"
     tRNA            complement(1657..1743)
                     /locus_tag="QBA20_002059"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="QBA20_002060"
     tRNA            complement(1764..1839)
                     /locus_tag="QBA20_002060"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="QBA20_002061"
     tRNA            complement(1898..1974)
                     /locus_tag="QBA20_002061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="QBA20_002062"
     CDS             complement(2077..3462)
                     /locus_tag="QBA20_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ6292600.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="QBA20_002063"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="QBA20_002063"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDQ6292601.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="QBA20_002064"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="QBA20_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDQ6292602.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="QBA20_002065"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="QBA20_002065"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDQ6292603.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="QBA20_002066"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="QBA20_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDQ6292604.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="QBA20_002067"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="QBA20_002067"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDQ6292605.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="QBA20_002068"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="QBA20_002068"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDQ6292606.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="QBA20_002069"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="QBA20_002069"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDQ6292607.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="QBA20_002070"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="QBA20_002070"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDQ6292608.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="QBA20_002071"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="QBA20_002071"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDQ6292609.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="QBA20_002072"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="QBA20_002072"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDQ6292610.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="QBA20_002073"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="QBA20_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDQ6292611.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="QBA20_002074"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="QBA20_002074"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDQ6292612.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="QBA20_002075"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="QBA20_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDQ6292613.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="QBA20_002076"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="QBA20_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDQ6292614.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="QBA20_002077"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="QBA20_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDQ6292615.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="QBA20_002078"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="QBA20_002078"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDQ6292616.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="QBA20_002079"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="QBA20_002079"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDQ6292617.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="QBA20_002080"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="QBA20_002080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDQ6292618.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="QBA20_002081"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="QBA20_002081"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDQ6292619.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="QBA20_002082"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="QBA20_002082"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDQ6292620.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="QBA20_002083"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="QBA20_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDQ6292621.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="QBA20_002084"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="QBA20_002084"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDQ6292622.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="QBA20_002085"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="QBA20_002085"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDQ6292623.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="QBA20_002086"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="QBA20_002086"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDQ6292624.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="QBA20_002087"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="QBA20_002087"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDQ6292625.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="QBA20_002088"
     CDS             complement(31267..32913)
                     /locus_tag="QBA20_002088"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDQ6292626.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="QBA20_002089"
     CDS             complement(32916..32966)
                     /locus_tag="QBA20_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292627.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="QBA20_002090"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="QBA20_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDQ6292628.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..35022
                     /locus_tag="QBA20_002091"
     CDS             33502..35022
                     /locus_tag="QBA20_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDQ6292629.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="QBA20_002092"
     CDS             complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="QBA20_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDQ6292630.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35504..36343
                     /gene="hdfR"
                     /locus_tag="QBA20_002093"
     CDS             35504..36343
                     /gene="hdfR"
                     /locus_tag="QBA20_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDQ6292631.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36439..36514)
                     /locus_tag="QBA20_002094"
     tRNA            complement(36439..36514)
                     /locus_tag="QBA20_002094"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36479..36481),aa:Trp,seq:cca)
BASE COUNT         8918 a         9998 c         9201 g         8403 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 gcgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct catcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acgggcgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg gaatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccgatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg
    34621 ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc cggcattttg
    34681 agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc
    34741 agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata
    34801 cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaagaaac gctgatccat
    34861 ctggggttat cgattcgtgc ctggcagcgg ttactgaaag ttgcacgaac cattgctgat
    34921 attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg
    34981 attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc
    35041 tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc
    35101 ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg
    35161 ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga gtttctcttc
    35221 ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc
    35281 aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg
    35341 tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa
    35401 ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca
    35461 aaacatttaa atcataacga caaataattt tgcggagagc actgtggata cggaattgtt
    35521 aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta
    35581 tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa
    35641 ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc
    35701 ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg cgcatacctc
    35761 acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca
    35821 gtggttggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg
    35881 aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat
    35941 taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctggggt atttcacttt
    36001 agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc tgcgactgga
    36061 gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagttcccat
    36121 tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac
    36181 ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac
    36241 aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat
    36301 tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatctgaat ggctggcaag
    36361 gatgccggta gaaggattta cttcggagag gattatttca gataaaaaaa atccttagct
    36421 ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg
    36481 gagaccggtg ctctaccaat tgaactacgc ccctaattag
//
LOCUS       DAOFMX010000038        34955 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.038, whole genome shotgun sequence.
ACCESSION   DAOFMX010000038 DAOFMX010000000
VERSION     DAOFMX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.038"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..815)
                     /locus_tag="QBA20_002095"
     CDS             complement(<1..815)
                     /locus_tag="QBA20_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HDQ6292632.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="QBA20_002096"
                     /pseudo
     CDS             907..993
                     /locus_tag="QBA20_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1398..1874)
                     /locus_tag="QBA20_002097"
     CDS             complement(1398..1874)
                     /locus_tag="QBA20_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDQ6292633.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="QBA20_002098"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="QBA20_002098"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDQ6292634.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QMAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="QBA20_002099"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="QBA20_002099"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDQ6292635.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="QBA20_002100"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="QBA20_002100"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDQ6292636.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="QBA20_002101"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="QBA20_002101"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDQ6292637.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="QBA20_002102"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="QBA20_002102"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDQ6292638.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="QBA20_002103"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="QBA20_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDQ6292639.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="QBA20_002104"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="QBA20_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDQ6292640.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="QBA20_002105"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="QBA20_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDQ6292641.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="QBA20_002106"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="QBA20_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDQ6292642.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGLTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="QBA20_002107"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="QBA20_002107"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDQ6292643.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="QBA20_002108"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="QBA20_002108"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDQ6292644.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="QBA20_002109"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="QBA20_002109"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDQ6292645.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="QBA20_002110"
     CDS             13849..14553
                     /locus_tag="QBA20_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDQ6292646.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="QBA20_002111"
     CDS             14758..15471
                     /locus_tag="QBA20_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDQ6292647.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISYGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="QBA20_002112"
     CDS             complement(15507..16463)
                     /locus_tag="QBA20_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDQ6292648.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="QBA20_002113"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="QBA20_002113"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDQ6292649.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="QBA20_002114"
     CDS             complement(17701..18462)
                     /locus_tag="QBA20_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDQ6292650.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="QBA20_002115"
     CDS             18595..19005
                     /locus_tag="QBA20_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDQ6292651.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="QBA20_002116"
     CDS             complement(18967..20073)
                     /locus_tag="QBA20_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6292652.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="QBA20_002117"
     CDS             complement(20084..21217)
                     /locus_tag="QBA20_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6292653.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="QBA20_002118"
     CDS             complement(21210..22946)
                     /locus_tag="QBA20_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6292654.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="QBA20_002119"
     CDS             complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="QBA20_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDQ6292655.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="QBA20_002120"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="QBA20_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDQ6292656.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26024
                     /gene="rhlE"
                     /locus_tag="QBA20_002121"
                     /pseudo
     CDS             24837..26024
                     /gene="rhlE"
                     /locus_tag="QBA20_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="QBA20_002122"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="QBA20_002122"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HDQ6292657.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="QBA20_002123"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="QBA20_002123"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDQ6292658.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="QBA20_002124"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="QBA20_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDQ6292659.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31158
                     /gene="hcxB"
                     /locus_tag="QBA20_002125"
                     /pseudo
     CDS             30114..31158
                     /gene="hcxB"
                     /locus_tag="QBA20_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
     gene            complement(31387..31647)
                     /locus_tag="QBA20_002126"
     CDS             complement(31387..31647)
                     /locus_tag="QBA20_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ6292660.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31912..32178)
                     /gene="ybiI"
                     /locus_tag="QBA20_002127"
     CDS             complement(31912..32178)
                     /gene="ybiI"
                     /locus_tag="QBA20_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDQ6292661.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32252..32929)
                     /gene="ybiX"
                     /locus_tag="QBA20_002128"
     CDS             complement(32252..32929)
                     /gene="ybiX"
                     /locus_tag="QBA20_002128"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDQ6292662.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32971..34955)
                     /locus_tag="QBA20_002129"
                     /pseudo
     CDS             complement(32971..34955)
                     /locus_tag="QBA20_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8285 a         8961 c         9441 g         8268 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccatctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga acccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttaccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggtttgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccccgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgta tggacgaaat
    15301 aagctccttg aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaac gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgcggt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgga aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgcgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcgc tggggattga gaaagcgcat cagcacggta ttgctgccgt ggcgctacat
    30421 aactcgcatc atatcggccg tatcggttac tgggcggagc agtgttcagc ggcggggttt
    30481 gtctctatcc actttgttag cgtggtcggt attccaatgg tcgcgccgtt ccacggtcgc
    30541 gacagccgct ttggcaccaa tcccttctgt gtggttttcc ctcgtaaaga taatttcccg
    30601 ctgttgcttg attacgccac cagcgccatt gcatttggca aaacccgcgt cgcctggcat
    30661 aaaggcgtcc ccgtgccgcc aggttgcctg attgacgtta acggcgtgcc gacgaccaat
    30721 ccggcggtaa tgcaggagtc gccgttgggg tcgctgttga cctttgccga acataaaggc
    30781 tacgcccttg cagcgatgtg tgaaattctt ggcggggcgc tttctggcgg taaaacgacg
    30841 catcaggaaa cgttgcaaac cagccccgat gccattctta actgcatgac cactatcatc
    30901 atcaacccgg aactgttcgg cgcgccggat tgtaacgcgc agaccgaagc ctttgccgag
    30961 tgggtgaaag cctcgccgca tgatgacgat aagccgattt tgctaccggg cgagtgggaa
    31021 gtgaacacgc gtcgcgaacg gcaggagcag gggattccat tggatgcggg aagctggcag
    31081 gccatttgtg atgcggcgcg gcagattggt atgtcggaag agacgttgca ggctttctgt
    31141 cagcagttag ccagctaaaa aaaagcccgt ccagtggcgg acgggcaaac aagggtaaca
    31201 taggatcaat gagggttaga gcatatgcgt ctgtcggcaa acagacaggg aaatacttgt
    31261 gctggacgta gcgtaaacgc ctgatccgtc caacggttcg gattttgtag gcctgataag
    31321 acgcggtagc gtcgcatcag gcatctgttg ccggatgcgg cataaacgcc ttatccgtcc
    31381 tacgaattac ttataaatta ccgcagtacc gcttaattta ttgttgttgg tggcggaagt
    31441 gatgctgtat ccactggcac cggctgccgc cgctttctca gccagtttcg cttccagggc
    31501 atcgagggtg gatgcgccat cggcagaaac cacgccgatt ttattcatgt tctgtgcctg
    31561 ggatgccgtt accggttccg ccgcgaacac gccaaatgac agagttgaaa gagccatagc
    31621 agcaacaaca gtattgatag ttttcataat taatctctcg caggtgattt ttgtataagt
    31681 gacgttgttt cgtcgatgtg atgagtatca cgttttttta cgagagataa aatcgaagag
    31741 aattgaccgc cttgttcaaa taaattgatt gatatctaac atactgataa accatttatt
    31801 tgttgtactt atggacttgt tttatgccgc gtggcagtca cagcagcgtt aaaggcacgg
    31861 aatgccacgc aaaatacaaa ttatactcgc tatgcatctc gccagttgta gttaacgtaa
    31921 ctgtctgtct ttcgaacctc tgcgattata tcctgtatac gctggttttt gtaaatcttt
    31981 ctcctgctga caatgaatac ataagcgcac gccaggaatg gcttcccgac gggcttgcgg
    32041 gatgggggca ccgcactctt cacattcata caggctttcg ccacgcggaa tttcaccccg
    32101 agcgcgggca atcgcatctt caattgtact gttgatctgt tcgttgacgg cgtcatcgtt
    32161 agcccaagcg gatgccatat cgtcctcccc atatcaatgc ttgtacagtt aagtgtagct
    32221 aatataggga cgaacctggg cagttcaagc gtcagatctc cgaccattcc cgcagcagat
    32281 tatgataaag attgagcagc gacaggatct cttcattttc accatagcgg cttttcagcg
    32341 actgaatatt tttgtccagt tcaaacagca tggcgcgctt tttatcatcg cggatcatcg
    32401 actggatcca cataaatgat gccactcgta cgccgcgggt tacgggtgtc acgcaatgca
    32461 ggctgctgga gggatacaac accagatcac ctgccgggag ttttacccga tgttgtccaa
    32521 aggtgtcatt aacgaccagt tcgccgccgt cgtagctttg tggatcgctt aaaaacagcg
    32581 tggcagaaag gtcagtgcgc atccagccgt tttgtggatg gctgcgtact gcgccatcca
    32641 catggaaacc ataggtttca ttgttctgat agcgattaaa cagcggcgtg gaaagggtac
    32701 gcggcaaggc agcggcaaag aacaatgcat gttggttaac cgcgttcagc acctcatttt
    32761 gcagtgcggc gtataacgcg ctgcgggtgt ctacttgctg attgttctta acttgtgcac
    32821 cttgtgctcc ggtggtgacg cgtccatcca cccattcggc ttgttccagt tgttcgcgaa
    32881 aatgggcgac gtcctgtggc gataacacgc cgggaatgtg gtacatcata caagttctcc
    32941 aaaaagtggg gcctgcgccc cacatctgaa tcagaaatgc atattggctg tgagcaagaa
    33001 ggttcttggc tcgcccgggt gataacggta gccgctcttg ttgattgagg cgacgtaatc
    33061 ggtatcaaac aggttgtaaa cgtttagctg gaagtcgaga ttgcgattaa cccgataccc
    33121 cagtttggca tcggcgaccc agtaaccttc tgtaaatgct ggcgttccca ccgcgccgtc
    33181 tgaaccttta tgtatactgc cgatatagcg tgcgcccgcg ccaacagaga tatcatcggt
    33241 tgcctgatat tggctccata aggtgaaggc gtgctccggg gtatacggca gcgatgagga
    33301 accatcctgg gcaacatctt tgccgttttt gatggttgct ttttgctggg tatagccgcc
    33361 aatcacctgc cacgcgggag tgatattccc tgccacggat atctcatagc cttcgacgcg
    33421 tttcttaccg tattgcgagt aagtcccgtc atcattttgc tcaacttcat tttcgatatc
    33481 agtacggaac agcgcggcgg tgagcaacag gcgtttatcc agaacctgcc atttggtgcc
    33541 aatctcgctg gtgttggctt tttgcggttt aaaatcggtg cggttggcac tgttaccact
    33601 gccagactgc gcaagggcga agttgttgcc gcccggaggc tgctgggaaa cggcatagtt
    33661 aatatagaca ttgccgtttt ccgtcaggtg gtacagcgcc ccggctttcc agttcaccag
    33721 attgcccgac ttggcggtgt cgacggtggt gactggagaa ccttttgcca caccagctgg
    33781 gcaggtgatg gcaccgcgtc cgctgccgcc gcaggcggtg gcactgtcat attcagtatg
    33841 ataattatcc agacggatcc cgccgttcag ctcaaaatca cgggtgattt gcagcgtatc
    33901 gaaggcgtaa attgcgaagg tatccgtctg accattggcg tttgcgccgt tgcgcgtcag
    33961 gccgccggga tgaattctgc tgtcaggatg ataaatattt accgccggta acgtcaccgg
    34021 attaacgcca tagttagtct gcgtttcacg ggtaaattcc acgccggtac tgacatcatg
    34081 accgatagaa ccggtataga acgtcgaggt caggttggtc tggttggtga gaattttatt
    34141 actcacatct ttggtattcg ccgtgcgtga ctaggtccag ctattcacat cgctggtggg
    34201 ctgagtaata ttcgacgccc cgcccataat cgccgtcatc aggtaatcct gctttacgcg
    34261 cgaccaacgg gtagtattgc gaatggtggt gttatcgttg atgtcgtgct caaaacgcat
    34321 ggtggcggtg tcggtggtcg aatcgtcgta atcggaatcc gtgccgtaaa agttatgagt
    34381 atcaactttt ccggaatgat tcagggccgc cgctcccgca gatggggcag aatagcccgg
    34441 caaaccgatg gtcggaatgc cgccgtctgg cgtgttgtgc tgagtgacat gcagataatt
    34501 aagatacaaa cgatttgctg taccaaggcc aaaagcgaca gaaggggcga cgccgtaacg
    34561 ctcatttttg actttgtcgc gtccggcatc gtgggttttt tcgcccatca cattcaggcg
    34621 caccgcggta gtatcaccaa tgacctgatt gacgtccagc gtgccgcggc ggaaccaggc
    34681 gctgccaata ctggcggagg cgtcaatgcc ggaatcattg cgcggctgct tgctgatcat
    34741 attgatcgag cctgtcggtg cgctgcgccc gtagtcggtg ccggacggcc ctttaatcac
    34801 ttcgacctgc tcggtattga aggtgtcgcg cgagacgctg ccgatatcgc gaataccatc
    34861 aatataaata ctgttagagg tatcggcacc acgcatataa atggcgtcgc cagtggtgga
    34921 gttaccgttc tcacccgcaa aaaacgcacc cacgc
//
LOCUS       DAOFMX010000039        34918 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.039, whole genome shotgun sequence.
ACCESSION   DAOFMX010000039 DAOFMX010000000
VERSION     DAOFMX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.039"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            872..3157
                     /gene="nrdA"
                     /locus_tag="QBA20_002130"
     CDS             872..3157
                     /gene="nrdA"
                     /locus_tag="QBA20_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDQ6292663.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3391..4521
                     /gene="nrdB"
                     /locus_tag="QBA20_002131"
     CDS             3391..4521
                     /gene="nrdB"
                     /locus_tag="QBA20_002131"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDQ6292664.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4521..4775
                     /gene="yfaE"
                     /locus_tag="QBA20_002132"
     CDS             4521..4775
                     /gene="yfaE"
                     /locus_tag="QBA20_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDQ6292665.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="QBA20_002133"
     CDS             complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="QBA20_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDQ6292666.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5658..5900
                     /locus_tag="QBA20_002134"
                     /pseudo
     CDS             5658..5900
                     /locus_tag="QBA20_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5942..7018)
                     /gene="glpQ"
                     /locus_tag="QBA20_002135"
     CDS             complement(5942..7018)
                     /gene="glpQ"
                     /locus_tag="QBA20_002135"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDQ6292667.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7023..8381)
                     /gene="glpT"
                     /locus_tag="QBA20_002136"
     CDS             complement(7023..8381)
                     /gene="glpT"
                     /locus_tag="QBA20_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDQ6292668.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8654..10282
                     /gene="glpA"
                     /locus_tag="QBA20_002137"
     CDS             8654..10282
                     /gene="glpA"
                     /locus_tag="QBA20_002137"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDQ6292669.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10272..11531
                     /gene="glpB"
                     /locus_tag="QBA20_002138"
     CDS             10272..11531
                     /gene="glpB"
                     /locus_tag="QBA20_002138"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDQ6292670.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLIDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11528..12718
                     /gene="glpC"
                     /locus_tag="QBA20_002139"
     CDS             11528..12718
                     /gene="glpC"
                     /locus_tag="QBA20_002139"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDQ6292671.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12911..13861
                     /locus_tag="QBA20_002140"
     CDS             12911..13861
                     /locus_tag="QBA20_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDQ6292672.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13902..14372)
                     /locus_tag="QBA20_002141"
     CDS             complement(13902..14372)
                     /locus_tag="QBA20_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDQ6292673.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14427..15629)
                     /locus_tag="QBA20_002142"
     CDS             complement(14427..15629)
                     /locus_tag="QBA20_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDQ6292674.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15716..16258)
                     /locus_tag="QBA20_002143"
     CDS             complement(15716..16258)
                     /locus_tag="QBA20_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292675.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16537..16962
                     /gene="nudI"
                     /locus_tag="QBA20_002144"
     CDS             16537..16962
                     /gene="nudI"
                     /locus_tag="QBA20_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDQ6292676.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(17001..17603)
                     /locus_tag="QBA20_002145"
     CDS             complement(17001..17603)
                     /locus_tag="QBA20_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDQ6292677.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17911..19050
                     /gene="arnB"
                     /locus_tag="QBA20_002146"
     CDS             17911..19050
                     /gene="arnB"
                     /locus_tag="QBA20_002146"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDQ6292678.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19054..20022
                     /gene="arnC"
                     /locus_tag="QBA20_002147"
     CDS             19054..20022
                     /gene="arnC"
                     /locus_tag="QBA20_002147"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDQ6292679.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20022..22004
                     /gene="arnA"
                     /locus_tag="QBA20_002148"
     CDS             20022..22004
                     /gene="arnA"
                     /locus_tag="QBA20_002148"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDQ6292680.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            22001..22891
                     /locus_tag="QBA20_002149"
     CDS             22001..22891
                     /locus_tag="QBA20_002149"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDQ6292681.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22891..24543
                     /gene="arnT"
                     /locus_tag="QBA20_002150"
     CDS             22891..24543
                     /gene="arnT"
                     /locus_tag="QBA20_002150"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDQ6292682.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24540..24875
                     /gene="arnE"
                     /locus_tag="QBA20_002151"
     CDS             24540..24875
                     /gene="arnE"
                     /locus_tag="QBA20_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDQ6292683.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24875..25261
                     /gene="arnF"
                     /locus_tag="QBA20_002152"
     CDS             24875..25261
                     /gene="arnF"
                     /locus_tag="QBA20_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDQ6292684.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25255..25527)
                     /gene="pmrD"
                     /locus_tag="QBA20_002153"
     CDS             complement(25255..25527)
                     /gene="pmrD"
                     /locus_tag="QBA20_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDQ6292685.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25632..26987)
                     /gene="menE"
                     /locus_tag="QBA20_002154"
     CDS             complement(25632..26987)
                     /gene="menE"
                     /locus_tag="QBA20_002154"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDQ6292686.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLPVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26984..27946)
                     /gene="menC"
                     /locus_tag="QBA20_002155"
     CDS             complement(26984..27946)
                     /gene="menC"
                     /locus_tag="QBA20_002155"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDQ6292687.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27946..28803)
                     /gene="menB"
                     /locus_tag="QBA20_002156"
     CDS             complement(27946..28803)
                     /gene="menB"
                     /locus_tag="QBA20_002156"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDQ6292688.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28818..29576)
                     /gene="menH"
                     /locus_tag="QBA20_002157"
     CDS             complement(28818..29576)
                     /gene="menH"
                     /locus_tag="QBA20_002157"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDQ6292689.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29573..31243)
                     /gene="menD"
                     /locus_tag="QBA20_002158"
     CDS             complement(29573..31243)
                     /gene="menD"
                     /locus_tag="QBA20_002158"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDQ6292690.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31332..32627)
                     /gene="menF"
                     /locus_tag="QBA20_002159"
     CDS             complement(31332..32627)
                     /gene="menF"
                     /locus_tag="QBA20_002159"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDQ6292691.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32706..33011)
                     /gene="elaB"
                     /locus_tag="QBA20_002160"
     CDS             complement(32706..33011)
                     /gene="elaB"
                     /locus_tag="QBA20_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDQ6292692.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33066..33527)
                     /locus_tag="QBA20_002161"
     CDS             complement(33066..33527)
                     /locus_tag="QBA20_002161"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ6292693.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33592..34509
                     /gene="rbn"
                     /locus_tag="QBA20_002162"
     CDS             33592..34509
                     /gene="rbn"
                     /locus_tag="QBA20_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDQ6292694.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLARDAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34700..34888
                     /locus_tag="QBA20_002163"
                     /pseudo
     CDS             34700..34888
                     /locus_tag="QBA20_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8537 a         9346 c         8652 g         8383 t
ORIGIN      
        1 atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg
       61 atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga gaaaagagat
      121 gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat ccgcatatta
      181 atctgaccat cattttatat aagacggcat atttaaacgc attatacaat atgggttatc
      241 tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt tgatgaataa
      301 tatcagtgcg tcataattca agttaataac cttaagggat accagttata tttaaactaa
      361 attaaagtaa tgaataattt tcttataata taaggtaaat taacaaaatg gcttcgcatt
      421 taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgcttat
      481 atattgacca caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc
      541 aatcattagc aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt
      601 tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg
      661 acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa
      721 gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc aaacataccc
      781 cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc
      841 ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg
      901 cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc
      961 agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta
     1021 tgacggtatc aagacctctg acatccacga aactattatc aaggctgccg cagacctgat
     1081 ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg
     1141 taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt
     1201 cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca
     1261 gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca
     1321 gctggaaggc aaatatctag tacagaaccg cgtgaccggc gaaatctacg aaagcgccca
     1381 gttcctttat attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct
     1441 gcaatacgtg aagcgttttt atgacgcggt ttccacattt aaaatttcgc tgccgacgcc
     1501 aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg
     1561 cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgtgaaat atgtttccca
     1621 gcgtgccggg atcggcatca acgccggacg tattcgtgcg ctgggcagcc cgattcgcgg
     1681 tggcgaagcg ttccataccg gctgtatccc gttctacaaa catttccaga cagcggtgaa
     1741 atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca
     1801 tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag gcaaccgcgt
     1861 gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgcc tgctgaaagg
     1921 tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc
     1981 cgatcaggaa gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa
     2041 gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg
     2101 tcgtatctat attcagaacg ttgaccactg taatacccat agtccgtttg atccgaccat
     2161 cgcgccagtt cgccagtcta acctgtgcct ggaaattgcc ctgccgacca aaccgctgaa
     2221 cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg
     2281 cgcaattaat agcctggatg aactggaaga gctggcgatt ctggcggttc gtgcacttga
     2341 cgcgctactg gattatcagg attacccgat cccggccgcc aaacgtggag cgatggggcg
     2401 tcgtacgctg ggtattggtg tgattaactt cgcttactat ctggcgaagc acggtaaacg
     2461 ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta
     2521 cctgctgaaa gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt ggtttaacga
     2581 aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggatgccat
     2641 cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg
     2701 cctgcgtaac tccacgcttt ctgccctgat gccgtccgag acctcttcgc agatctctaa
     2761 cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg
     2821 tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg
     2881 ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga
     2941 tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc
     3001 gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta
     3061 ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca
     3121 ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcgccgt
     3181 aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc cagcgtcgca
     3241 tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc tcggatttgt
     3301 aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa aatgccttat
     3361 ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc acagacgaaa
     3421 aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc tcgctacgat
     3481 cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt cttctggcgt
     3541 ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc ggagcacgaa
     3601 aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat tcagggtcgt
     3661 agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac ctgggtcgaa
     3721 acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat tcgtaatatc
     3781 gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat ccagaaacgt
     3841 gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta ctggcacctt
     3901 ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct gcgcgagctg
     3961 aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat ccgcttctac
     4021 gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg taacgccaag
     4081 attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca gcatatgctg
     4141 aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga agaatgtaag
     4201 caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg ggcggattat
     4261 ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca gtacgttgaa
     4321 tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca gacgcgctcc
     4381 aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt tgctccgcag
     4441 gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga caccgacgat
     4501 ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc acacaactgc
     4561 tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat gtggcggttg
     4621 agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc gcaggtcagg
     4681 tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg ccctgttgtt
     4741 gccgggcaaa aggcgatatt gaaatcgagt tgtgatcggg tactagcgcc agatacgacg
     4801 cttacgcgta ttatctggcc tacaacgatt acatggcgta gtaatacgct ttcacctgct
     4861 cccagtccgc tttagggatc ggctccagat atttttccag ctggcggaag tcatgattaa
     4921 tcgctttatc gcgacgcaag cggcgacgac ttttctccag atcaagaaaa ccagcttctg
     4981 ccttaccttc tgttttcaca tagatatggc gaacatagca acagccatgc tgacgattaa
     5041 cgctatgcat tttcttaaac gccagtgcca ctgctttcaa catggcttgc cgtacttcgt
     5101 cagaataagg cgatactgca tgccgggcat accagtcagc aatgctgatg aaccccgcca
     5161 tatcttcagt caccagcaac gcgcgccatt caccctcaat tttcaccgct tcaccaaaaa
     5221 cgatcttcgg cacgatgaca ccggcccgtt ccagttcttt aattaccgca acctcacgga
     5281 caatcgttgg tcggccgaac ggataacgta ctgaatgaaa cagatgatgc gtcatgcgct
     5341 ttacatacag ctttttgccg ttgcgctcga cgcattgcac cccgctcata ccattacggc
     5401 gatagttagg ctcttcaacc cagtcgccct ctgttgccca ccagtgatta aattcgtcgt
     5461 actttgctga aactgccata ccctatcgcc tgtcattttt attaacgaca atgactatag
     5521 gtggttacct gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttc ttttgccgct
     5581 acaggtgtga caccgctttt gcccttcatt tgcagcgcat agattccacc agccactacc
     5641 actaatagtg cgcccagcag ataaaatgtg aatttaatca gtcggataca gcgatgaatt
     5701 ttattcgaca gggattaggc atcgccttac aaccagagtt aacgctgaaa agcattgcag
     5761 gtgaattgtg ttccgttcct cacgaaccaa ccttctatcg acagatttcg ttgctgacta
     5821 aagaaaagcc ggtagaaggc agtccactgt ttttactaca aatgtgcatg gaacaattag
     5881 tggcgattgg aaaaatttga tatgagacag gatggcgcat gaacgccatc ctgcagtaac
     5941 attactcttt attaagaaat tttactgcct tatcagggaa atcagtaaac agcccattta
     6001 cacccgcttt gttatacaga gcatcatata actgattcac atcaggagtg tattcaggca
     6061 gtttatctga ccgcacggta taaggatgca ctaccagttt attctgctgg gcatcttgca
     6121 ccatgccagt gagtttgata ttacccggct gcgatgtctc ctcaatcaac atatggtaat
     6181 ccggaccaat accatctgca tattccgcca cctgtttcat ggctcccggc ttaaacatcc
     6241 agtcgtagtt gtaattaacc cagcttccat ccggctgttt ctgctgcgtt tcattccagt
     6301 cggtataggc aatcagctgt accagattga gctccatgcc cattttgggt tccagctcat
     6361 tcttaatacg cttcagctca tcagcatcaa aacattgcaa ataaacttta tcgtctttac
     6421 cggtgtaacc atatttcttc agcacttcca gcgtttttgc cgcaatatcc ttcccttcct
     6481 gatgatggaa ccacggcgct ttgatttctg gatagatacc gatatttttc ccggtagagt
     6541 gatttaaccc ctgaacaaat tcaatctctt cttcaaaggt gtgcacccgg aagtcggact
     6601 tacccattgg gaaacgcccc ggataagtct gtactttttt accgttttca atatcgaaac
     6661 cttcggtaaa tttcagcgac ttaatttcat ccagcgtgaa atctatcgcg tagtaacgac
     6721 cgtctttgcg cgcccgatcc gggaaacgat cggcaacatc agtaacacga tcgagataat
     6781 ggtcatgcag aacaaccaga tggtcgtctt tggtcatcac caaatcctgt tccagataat
     6841 ccgctccctg cgcatacgcc atcgcttttg ctggcagcgt atgctccggc aaatatccac
     6901 tggcaccgcg atgggcgatg actatttttt cgttgctgtc cgccgccatt gcactgcttc
     6961 ccatgactat agtgctcatc atgatcgcca tgctaaggtt tttcagcttc aatttcatgc
     7021 cattagcctc cgttgcgttt ttgcagtaat tgttcatggc gacgtttttc gccaatcatc
     7081 acaacaatca acaagataac cgccagaatg ctgccgccaa tcattaccat aaagccgcca
     7141 tcccagccga agaagtccac ggtgtagcca acaatcgcgc tcgccgccac cgaaccgccc
     7201 aggtaaccaa acagcccggt aaagcccgct gccgtacctg ccgctttttt cggtgccagt
     7261 tccagcgcat gcagaccgat cagcatcaca ggaccgtaga tcaggaagcc gataacaatc
     7321 atacaaatca tatcgacggt tgggttacct gccgggttca tccagtaaac gatagtcgcg
     7381 atggtcacca gtgtcataaa gaagacgccg gttgccccac ggttaccacg gaagactttg
     7441 tccgacatcc agccgcacag cagggtgccc ggaatacccg catactcata gaagaagtag
     7501 gcccaggagg atttatccag cgcgaaatgc ttaacctctt tcagataagt cggtgaccag
     7561 tcgaggatgc cgtaacgcag cagataaacg aacacgttgg cgatggcgat gtaccacagc
     7621 agtttgttcg gcagcacgta ctgcatgaag atttgcttcg cggtcagctc ctgttccgct
     7681 ttttcgttat agtcgtccgg ataatcattt ttgtactctt cgatcggcgg caagccacag
     7741 gattgcgggg tatcgcgcat catcgcaaag gcgaataatg ccaccagaat ggcgcagaaa
     7801 gcaggcatat agagcgccgc atgccagtca ttgaaccagg ccatccccag caggaacagc
     7861 agcggcggaa taccaccacc gacgttgtgc gcacagttcc acactgacac aatgccgcca
     7921 cgttctttct gcgaccacca gtgcaccata gtacgaccac acggcggcca ccccatcccc
     7981 tggaaccaac cgcagaggaa caacagtaca aacatcaccg caatgctcga cgtcgcccat
     8041 ggcacaaagc ccataaacaa catcactgcc gccgccagaa tcaaacctgc gggcaggaaa
     8101 acgcgcggat tcgagcgatc cgataccgaa cccatgatga atttcgaaaa tccataagca
     8161 atcgagatcc ccgaaagggc aaaacctaaa tcaccgcgtg agaatccctg ctcaaccaga
     8221 taaggcatag caagcgcaaa gttcttacga accaaatagt aagccgcata gccaaagaat
     8281 atccccagga aaatttgcca gcgcaatcga cgataagtcg gatcgatctc cgcggcaggt
     8341 aagcgcgctt tgtgtggcgc tggtttaaaa atactcaaca ttgatagcct ccgtggcccg
     8401 tggtcttatt tatgattaac agcctgattc agtgagagaa cctgccgttt cttgagttgc
     8461 cgcgatgtta agaaaacatt cataaattaa atgtgaattg ccgcacacat tattaaataa
     8521 gatttacaaa atgttcaaaa tgacgcatga aatcacgttt tactttcgaa tcatgagcga
     8581 atatgcgcga aatcaaacaa ttcatgtttt tactatggct aaatggtaaa aaacgaactt
     8641 cagagggata acaatgaaaa ctcgcgactc gcaatcaagt gacgtgatta tcattggcgg
     8701 cggcgcaacg ggagccggga ttgcccgcga ctgtgccctg cgcgggctgc gcgtgatttt
     8761 ggttgagcgc cacgacatcg caaccggtgc caccgggcgt aaccacggcc tgctgcacag
     8821 cggtgcgcgc tatgcagtaa ccgatgcgga atcggcccgc gaatgcatta gtgaaaacca
     8881 gatcctgaaa cgcattgcac gtcactgcgt tgaaccaacc aacggcctgt ttatcaccct
     8941 gccggaagat gacctctcat tccaggccac ttttattcgc gcctgcgaag aagcagggat
     9001 cagcgcagaa gctatagacc cgcagcaagc gcgcattatc gaacctgccg ttaacccggc
     9061 actgattggc gcggtgaaag ttccggatgg caccgttgat ccatttcgtc tgaccgcagc
     9121 aaacatgctg gatgccaaag aacacggtgc cgttatcctt accgctcatg aagtcacggg
     9181 gctgattcgt gaaggcgcga cggtgtgcgg tgttcgtgta cgtaaccatc tcaccggcga
     9241 aactcaggcc cttcatgcac ctgtcgtggt taatgccgct gggatctggg ggcaacacat
     9301 tgccgaatat gccgatctgc gcattcgcat gttcccggcg aaaggatcgc tgctgatcat
     9361 ggatcaccgc attaaccagc atgtgatcaa ccgctgccgt aaaccttccg acgccgatat
     9421 tctggtgcct ggcgatacca tttcgctgat tggtaccacc tctttacgta ttgattacaa
     9481 cgagattgac gataatcgag tgacggcaga agaggttgat attctgctgc gtgaagggga
     9541 aaaactggcc cccgtgatgg cgaaaacgcg cattttgcgg gcctattctg gcgtgcgccc
     9601 gctggttgcc agcgatgacg acccgagcgg acgtaacgtc agccgtggca tcgtgctgct
     9661 cgaccatgct gaacgcgatg gtctggacgg atttatcacc atcaccggtg gcaaactgat
     9721 gacctatcgg ctgatggctg aatgggctac cgacgcggta tgccgcaaac tgggcaacac
     9781 gcgcccctgt acgactgccg atctggcact gcctggttca caagaacccg ctgaagttac
     9841 cttgcgtaaa gtcatctccc tgcctgcccc gctgcgcggt tctgcggttt atcgtcatgg
     9901 cgatcgcacg cctgcctggc tgagcgaagg ccgtctacac cgtagcctgg tatgtgagtg
     9961 cgaagcggta actgcgggtg aagtgcagta cgcggtagaa aatttaaacg ttaatagcct
    10021 gctggattta cgccgtcgta cccgtgtggg gatgggcacc tgccagggcg aactctgcgc
    10081 ctgccgcgct gccggactgc tgcaacggtt taacgtcacg acgtccgcgc aatctatcga
    10141 gcaactttcc accttcctta acgaacgctg gaaaggcgtg caacccatcg cctggggaga
    10201 cgcactgcgc gaaagcgaat ttacccgctg ggtttatcag ggactgtgtg gtctggagaa
    10261 ggagcagaaa gatgcgcttt gatactgtca ttatgggcgg cggcctcgcc ggattactct
    10321 gtggcctgca actgcaaaaa cacggcctgc gctgtgccat tgtcacgcgt ggtcaaagcg
    10381 cgttgcattt ctcatccggt tcgctggatt tgctgagcca tctgccagat ggtcaacccg
    10441 tggcagacat tcacagtgga ctggaatctt tgcgtcagca ggcacctgca catccttact
    10501 ctcttctcgg gccacaacgc gtgctcgatc tcgcttgcca ggcgcaggca ttaatcgctg
    10561 aaagcggtgc gcaattgcag ggcagcgtag aactggctca ccagcggatt acgccgctcg
    10621 gcactttgcg tgcaacctgg ttaagttcgc cagaagttcc cgtctggccg ctacccgcga
    10681 agaagatatg tgtagtgggg ataagcgggc tgatagattt ccaggcgcat cttgcggcgg
    10741 cttcgttgcg cgaactggat ctttcggttg aaaccgcaga aatagagctg ccggaactgg
    10801 atgtcctgcg caataacgcc accgaatttc gcgcggtgaa tatcgcccgt ttccttgata
    10861 atgaagaaaa ctggccgctg ttacttgatg cgcttattcc tgtcgccaat acctgcgaaa
    10921 tgatcctgat gcccgcctgc ttcggcctgg ccgatgacaa actgtggcgt tggttgaatg
    10981 aaaaactacc ttgttcactg atgcttttgc caacgctgcc gccttccgtg ctgggcattc
    11041 gtctgcaaaa ccagttacag cgccagtttg tgcgccaagg tggagtgtgg atgccgggcg
    11101 atgaagtgaa aaaagtgacc tgtaaaaatg gcgtagtgaa tgaaatctgg acccgcaatc
    11161 acgccgatat tccgctacgt ccacgtttcg cggttctcgc cagcggcagt ttctttagtg
    11221 gcggactggt agcggaacgt aacggcattc gcgagccgat tctcggcctt gatgtgctac
    11281 aaaccgccac gcggggtgaa tggtataagg gcgatttttt tgcgccgcaa ccgtggcagc
    11341 agttcggtgt aaccactgat gagacgctac gcccgtcgca ggcagggcaa accattgaga
    11401 acctgtttgc catcggttcg gtgctgggcg gatttgatcc catcgcccag ggatgcggcg
    11461 gcggtgtttg tgccgtcagt gctttacatg ccgctcaaca gattgcccaa cgcgcaggag
    11521 gccaacaatg aacgacacca gcttcgaaaa ctgcattaag tgcaccgtct gcaccaccgc
    11581 ctgcccggtg agccgggtga atcccggtta tccagggcca aaacaagccg ggccggatgg
    11641 cgagcgtctg cgtttgaaag atggcgcact gtatgacgag gcgctgaaat attgcatcaa
    11701 ctgcaaacgt tgtgaagtcg cctgcccgtc cgatgtgaag attggcgata ttatccagcg
    11761 cgcgcgggcg aaatatgaca ccacgcgccc gtcgctgcgt aattttgtgt tgagtcatac
    11821 cgacctgatg ggtagcgttt ccacgccgtt cgcaccaatc gtcaacaccg ctacctcgct
    11881 gaaaccggtg cggcagctgc ttgatgcggc gttaaaaatc gatcatcgtc gcacgctacc
    11941 gaaatactcc ttcggcacgt tccgtcgctg gtatcgcagc atagcggctc agcaagcaca
    12001 atataaagac caggtcgctt tctttcacgg ctgcttcgtt aactacaacc atccgcagtt
    12061 aggtaaagat ttaattaaag tgctcaacgc aatgggtacc ggtgtacaac tgctcagcaa
    12121 agaaaaatgc tgcggcgtac cgctaatcgc caacggcttt accgataaag cacgcaaaca
    12181 ggcaattacg aatgtagagt cgatccgcga agctgtggga gtaaaaggca ttccggtgat
    12241 tgccacctcc tcaacctgta catttgccct gcgcgacgaa tacccggaag tgctgaatgt
    12301 cgacaacaaa ggcttgcgcg atcatatcga actggcaacc cgctggctgt ggcgcaagct
    12361 ggacgaaggc aaaacgttac cgctgaaacc gctgccgctg aaagtggttt atcacactcc
    12421 gtgccatatg gaaaaaatgg gctggacgct ctacaccctg gagctgttgc gtaacatccc
    12481 ggggcttgag ttaacggtgc tggattccca gtgctgcggt attgcgggta cttacggttt
    12541 caaaaaagag aactacccca cctcacaagc catcggcgca ccactgttcc gccagataga
    12601 agaaagcggc gcagatctgg tggtcaccga ctgcgaaacc tgtaaatggc agattgagat
    12661 gtccacaagt cttcgctgcg aacatccgat tacgctactg gcccaggcgc tggcttaaac
    12721 tcccttctga tgcccggtaa gcatgtggtt accgggcatt tttgcgtaca cgattccgtg
    12781 cccaatgtat gcgttgcaac gcagtgaaaa ttcctctgaa aacgtctcgc aaaggctgaa
    12841 actggcagat gtcaaaggcc tgggataacc gtaatgtcgc gtcatcataa atatcaggtg
    12901 acggacaacc atgaccgaat caacaacctc ctccccgcat gatgcggtat ttaaaacctt
    12961 tatattcaca cccgaaaccg cacgggattt tctcgaaata catttaccag aaccactgcg
    13021 caagctttgc aacctgcaaa ccttacgcct ggaacccact agttttattg aaaaaagttt
    13081 acgcgcttac tactcggatg ttttgtggtc cgtggaaacc agcgacggtg acggctatat
    13141 ctactgcgtg attgaacatc aaagctctgc agaaaagaat atggcttttc ggccaatgcg
    13201 ctatgccact gccgccatgc agagtcacct agataaaggc tatgacagag ttccgctggt
    13261 ggtgccattg ctgttttatc atggcgaaac atcgccctac ccgtactcac ttaactggct
    13321 ggatgagttt gacgatccgc aacttgcccg gcagttgtac accgaagctt ttccgttggt
    13381 ggatatcacc atcgtacctg acgatgagat catgcaacat cggcgtatag ctctgctgga
    13441 actgattcaa aagcatattc gcgaccacga tttaatcggc atggtcgaca ggatcaccac
    13501 gcttttggtt agaggcttca ctaatgacag ccagctacaa acactgttta attatctgct
    13561 gcaatgcggc gatacctccc gtttcacccg ttttattcag gagattgccg aacgttcacc
    13621 actacaaaag gagagattaa tgactattgc tgaacggcta cggcaggaag gacatcaaat
    13681 tggctggcaa gaaggtaaat tagtaggttt gcagcaaggt aaattagaag gtttgcagga
    13741 aggtatgcat gaacaagcca ttaaaattgc tttgcgcatg ctggaacagg gcattgatcg
    13801 tgaccaagtg ctcgcggcca cccagctaag cgaagccgat ctggcagcga ataaccacta
    13861 attaacacag gcccacagcc gatccccatg ggcctttgat atcaataact accttttatg
    13921 cgtggcccat ttttgcctga tttaaacatc gccagtcgtt gatccagggc atcgctgtag
    13981 agcatcgtgt caacgccaac agcgacaaag ttcgctcccc acgccaggca ttgctgcgcc
    14041 atatcaggag ccacagccag aaaaccagcc gctttacccg cagcacggat ccgccgaatg
    14101 ctggtttcaa taattcgctg cacttccggg tgcccggcgt tatccgggta gcccaacgac
    14161 gcagaaagat ccgctggtcc aataaacacg ccatcaatcc cttcgacgtc gaggatctcg
    14221 tccaggttat ccagtgccgt tttactttcc acctgcacta acagacaaag cgaatcgttg
    14281 acttgcgcca tgtaattctc aatgcgtccc cagcgcgcag cccgtgccac actggcccca
    14341 acaccacgct caccgtaggg agggttggac aaattttgcc ggagggggaa tgtgccggtg
    14401 cagtaaaccg gcacagagtg gcgaaatcag acggataaag tcatggactc aacaacctca
    14461 atccagccat gctcactagc gatgtcctgg ccgtttaacc aacggcgtag catattcagt
    14521 gccatcattg cgcacacttc ctgacggata gccaggctgt agcgtgtagt gctgaaacgc
    14581 acacgcagag cgaaagtgcc gtctggcgtg gctagcgcaa agttgagatg ctcgttctcg
    14641 aaacccgaaa cagccagtgc cagcccggca aaatggttgg cccgccgttc tgtaatccag
    14701 tgcgcagttt gcgccagggt ttcttcctgt gaaggaatca cttcacacgc cagcaatgga
    14761 gcacctgcgc gagaaagttg caaagccaat aaaccaccgg tgaattgctc gctcaacgtc
    14821 aggctgaact ggcggttttg caattcgcga ctgatctgcg cgggcagtcc ttcggtgcct
    14881 tcgaaaatca cgctctgtcc ggcaacacgt ttaacgtcca gccacagttt ttccatcgcc
    14941 tgttgctcgc ttgccggtcc ggtgagtttc agttcgatga tcggcattga ggagcgatag
    15001 cccattgtta cgcccggcgg cagttgtaga gtgtccaggc tttgtgccag atcgctttcc
    15061 gaacgaccaa aggtagttaa acgcagacaa accggcggct gtggtaaaga aaaacgctcg
    15121 cgcaggcgcg gcaggatttc gtgctcgacc atcaccttaa attctgacgg tacgccggga
    15181 gtaaagaaca tcaggcaacg attaagctgc acggcaaaac cacaggcggt gcctaccggg
    15241 ttattgataa attcagcact ggcaggcagc tccgcttgtt tacggttgct cggtgccatt
    15301 actcgtccac gttcgtggaa atagcgttcc atctctttga gccaggcttc atgcagcacc
    15361 aggccttcac cttttgctgt cgcagcggcg agtgcgctta aatcatcgct ggtcggtccc
    15421 agcccgccgt taacgatcag cacatcggcg tgctgactac gttcgcgaag aatggtgact
    15481 aagtcatcaa ggttatcccc caccgtattg cggcgagata atggcagccc ctgatgaaag
    15541 aaaaaatcgg ccagccaggc agcgttagtg tcaacgattt gcccgtataa cacttcatcc
    15601 ccggtggata gcatttccac ttttaacatg gttatctcct gcttttgtta tcgaatcact
    15661 ataacgcaaa gggagataag gcgtagtaag aaactgaatg ccgcgccgga agggatcaga
    15721 aactggcgtt aaccccaaca tacaggccgt cagccacagc gttgtcgcgg ttaccgtctt
    15781 tacccgacag attcaggtag cgataacccg cctcaatact gactggacgc ataatggtgt
    15841 aacgcgcgcc agcattcgct tcctcataac tttgaatacc gctggagagc gaatccggag
    15901 agtagtaata ctcgccaaac aaacggaagc tgttgccaat tttccactgc aaaccacctc
    15961 ctaccgctgc ggcataacct tcatcgccgt aattcgggtt ggtgtacacg ccttttccgc
    16021 caacggtcgc cattaacggc ccgagaggca gattcaaccc cagccccacg cccgcgacgt
    16081 caccgtcgtc gtcgttatgt gtccagttac cgcttaaagc caggcccgtc gattcagtcc
    16141 caaaaccgac accaatattg gtgtattctt tacccgcctg gccgctgatg ctcattgcat
    16201 tgaccgatgc agaaaccaac agcattccgg ccagacctgc tagcgcaatt tttttcatta
    16261 tcccctttcc cacaagcaat taatcaaaat tcccaaagct gcgggattgt actgcctggc
    16321 acagaggatt caatgagcga aaaaagggat ctagtcactt attgtaacta aatacttctt
    16381 aaataattaa acgccgaaaa aataaacaca ctttaccgat acacacaaaa ataataataa
    16441 gtgcagttat cattgcaaaa gagattactg tcactttcac atatttctga gttaccgtat
    16501 tctcatggtt tctttttatt tataaggaat acccctgtgc gacaacggac tattgtatgc
    16561 cctttgattc aaaatgatgg tgcttatttg ctgtgcaaaa tggccgacga tcgcggcgtt
    16621 ttccccggtc aatgggcgct ttcgggtggc ggcgtggagt ctggcgaacg aattgaagag
    16681 gcactacgcc gcgaaattcg cgaagaactg ggagaacagc tgcttttgac agaaatcacg
    16741 ccgtggacct tcagcgatga tattcgcacc aagacatatg cagatggtcg caaggaagag
    16801 atttatatga tttacctgat ttttgactgc gtttctgcca accgagaagt gaaaataaac
    16861 gaagagtttc aggactacgc gtgggtaaaa cctgaagatc tggtgcatta tgatttaaat
    16921 gttgccaccc gaaaaacgtt acgtttgaaa ggtcttcttt aacaatgatg acagccatca
    16981 ctggctgtca gattgaaaat ttagtggtta acgaattccc catccagata aactttgcct
    17041 ttttccacat gcatgactaa accatccaga taatcaggtt taaatatcgc atcacgctta
    17101 tttttagcaa tatatgtcag gcaatgatta tgggtgaaaa taacgatatt tttatcaggc
    17161 gctttgcttt gtaagtcctt aattgcacta taaatctcat taccgcactg aagaagtcgt
    17221 ttatctaccg tcagcttttt acccgcggaa aaccaggtcg ccgactgaat ggtccggacg
    17281 gtattactgg aataaagatc gaaatcaggg atgtcagcgc taaaagcgtt gcccagttca
    17341 cgggcatcct gggtaccttt aaccgtaata cctgttttat ctgacaagca ttgattggtt
    17401 gaacgatcgc aacgttcagc atggcgaaac aaaacgacaa ccgggtgctg ctgtgccagt
    17461 ctggccaatg ttttattgtc gatacgtggc aaaccattgc tactccaggc ggcatgagta
    17521 cccagtccgg caattgcagc gagcgccagt aaaatgatga aatatttttt tgacttcaat
    17581 gatgagcggc aaaaagctaa cacgtaaact ccacctatag acaagcgcaa ccagacaatt
    17641 accgtgaaat tgagcgacat ttctggcgat aattagcagt tggtgtaata ttaaaaatcc
    17701 tacgatgtcg gcaaaatgcc tcaaattttt gccaaatgca aagcctaaat aagaaaaaat
    17761 ataaaaattt caatatttac gtctaatatt agtttcttaa ggttaagtta atattctatc
    17821 cttaaaattt cgctccaaat ggcaaaatat acacaacact ctttatagca aatataagtg
    17881 gacaggtatt caatggcgga aggaaaagca atgtcagaat ttttgccttt ttcgcgacca
    17941 gcaatgggcg tggaggaact cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca
    18001 accggtccga agaatcaggc gcttgagcaa gccttttgcc agttgacggg aaatcagcat
    18061 gccatcgcgg tcagttcagc caccgccgga atgcatatca cgctaatggc gttggaaatt
    18121 ggcaagggcg atgaagtgat tacgccttcc ctgacctggg tttcaaccct caatatgatt
    18181 tccttgctgg gtgcaacgcc ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg
    18241 cctgaagcga tcgaatcagc cattacgcca cgcactaaag ccatcattcc ggtgcattat
    18301 gccggtgcgc cagcagatat tgacgccatt cgcgccattg gcgaacgtta cggcatcgca
    18361 gttatcgaag atgctgccca tgccgtcggt acgtattaca aagggcgaca tattggcgca
    18421 aaaggtaccg ctattttttc atttcatgcc attaaaaata ttacctgtgc tgaaggtggc
    18481 ctgattgtaa ctgataatga aaaccttgcc cgccagctac ggatgctgaa atttcacggt
    18541 ctgggtgtcg atgcctatga cagacaaacc tggggccgtg caccgcaggc tgaagtctta
    18601 acaccgggct ataagtacaa tctgaccgat attaacgccg cgattgccct gacacagtta
    18661 gtcaaattag agcacctcaa cacccgtcgg cgcgaaattg cccagcaata tcagcaagca
    18721 ctggcagctc tcccctttca gccattaagc cttcccgcct ggccgcacgt tcacgcctgg
    18781 catctgttta ttattcgtgt cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg
    18841 gaagcgttaa aagaaagagg cattggtacc gggttacatt tccgcgccgc tcacacacaa
    18901 aaatattatc gcgagcgttt tcccacgctg tcgttaccga ataccgaatg gaatagcgaa
    18961 cgcatctgtt cgttgccgct gttcccggat atgactaccg ccgatgccga ccgcgtcatc
    19021 acagcccttc agcaactcgc aggacaataa gccatgtttg aaatccaccc tgttaagaaa
    19081 gtctcggtgg ttattcccgt ttataacgag caggaaagct taccggaatt aatcaggcgc
    19141 accaccacag cctgtgaatc gttggggaaa gagtatgaga tcctgctgat tgatgacggc
    19201 agtagcgata attccgcgca tatgctggtc gaagcctcac aagcggagaa cagccatatt
    19261 gtgtctattt tgattaaccg caattacggg caacattcag cgattatggc gggattcagt
    19321 cacgttactg gcgacttaat tattaccctt gatgccgatc tccagaatcc gccagaagaa
    19381 atcccccgcc tggtggcaaa agccgatgaa ggttacgacg tggtagggac tgtacgccag
    19441 aaccgccagg acagctggtt tcgtaaaaca gcttcgaaga tgattaaccg gcttattcag
    19501 cgcaccactg gcaaagcgat gggtgactac ggttgtatgc tgcgcgccta tcgccgtcat
    19561 attgtcgatg cgatgttgca ctgccatgaa cgcagcacct ttatcccgat tctggcgaat
    19621 atcttcgccc gccgtgcaat tgaaattcca gtacatcatg ccgagcgtga gtttggtgaa
    19681 tccaaataca gttttatgcg cctgattaat ttgatgtacg acctggtgac ctgccttacc
    19741 acaacgccgc tacgtatgct gagtctgctc ggcagcatta ttgcgattgg aggttttagc
    19801 attgcggtgc tgctggtgat tttacgcctg accttcggac cacaatgggc ggcagaaggc
    19861 gtctttatgc tatttgccgt gctgtttact tttattggcg ctcagtttat cggcatggga
    19921 ttactcggtg aatatatcgg caggatctac accgatgtcc gcgcccgccc ccgctatttt
    19981 gttcagcaag ttatccgtcc atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg
    20041 cctaccacga tatgggatgc ctcggtattg aagctctgct ggctgccggt tacgaaatta
    20101 gcgccatttt tacccatacc gataatcccg gtgaaaaagc cttttatggt tcggtggctc
    20161 atctggcggc ggaaagagac attccggttt atgcgccgga taacgttaat catccgctgt
    20221 gggtggaacg cattgcccaa ctgtcaccag aggtgatttt ctctttttat tatcgccatc
    20281 ttatttgcga cgagattttt cagctcgctc ccgcaggtgc atttaatctg cacggttcgc
    20341 tgttaccaaa atatcgtggt cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg
    20401 aaacgggcgt tacattgcac cgaatggtaa aacgtgccga tgctggggcc attgtggcgc
    20461 aactgcgcgt tgccattgcg ccagacgata tcgctattac gctgcatcat aaattgtgcc
    20521 atgccgcgcg ccagctactg gaacagacat tacccgccat taaacacggt aatattctgg
    20581 aaatcgccca gcgcgaaaac gaagccacct gttttggtcg cagaacgccg gatgacagtt
    20641 tccttgaatg gcataaaccg gcatccgtac tgcacaacat ggtacgtgcc gttgccgatc
    20701 cgtggccggg tgccttcagc tatgttggca atcagaaatt caccgtctgg tcgtcgcgtg
    20761 ttcatcctca tgccagcaaa gcacagccgg ggagcgtgat ttctgttgcg ccactgctga
    20821 ttgcctgtgg cgatggcgcg ctggaaatcg tcaccggaca ggcgggcgac ggcattacta
    20881 tgcagggctc gcaattagcg cagatgctgg gcctggtgca aggttcacgc ttgaatagcc
    20941 agcctgcctg caccgcccga cgccgtaccc gggtactcat cctcggggtg aatggcttta
    21001 ttggcaacca tctgacagaa cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg
    21061 atattggcag cgatgcgata agccgttttc tgaatcatcc acattttcac tttgtcgaag
    21121 gcgatatcag tattcattcc gaatggattg agtatcacgt caaaaaatgt gatgtcgtct
    21181 tgccgctggt ggcgatagcc acgccgattg aatatacccg caacccgctg cgcgtatttg
    21241 aactcgattt tgaagagaat ctgcgcatta tccgctactg cgttaagtac cgtaagcgaa
    21301 tcatcttccc gtcaacttca gaagtttatg ggatgtgtag cgataaatac ttcgatgagg
    21361 accattctaa tttaatcgtc ggcccggtga ataaaccacg ctggatttat tcggtgtcaa
    21421 aacaattact tgatcgggtg atctgggcct atggcgaaaa agagggttta cagttcaccc
    21481 tcttcctccc gtttaactgg atgggaccac gactggataa ccttaatgca gcgcgaattg
    21541 gcagctcccg cgctattacg caactcattc tcaatctggt agaaggttca ccaattaagc
    21601 taattgatgg cggaaaacaa aaacgctgct ttactgatat ccgggacggt atcgaggcgt
    21661 tgtaccgcat tatcgaaaat gcgggaaatc gctgcgacgg cgaaattatc aacattggca
    21721 atcctgagaa cgaagcgagc attgaggaac tgggcgagat gctgctggcg agcttcgaaa
    21781 aacatccgct gcgccatcat ttcccaccgt ttgcgggctt tcgcgttgtc gaaagtagct
    21841 gctactacgg caaaggatat caggacgtag agcatcgtaa accgagcatc cgcaatgccc
    21901 accgctgcct ggactgggag ccgaaaattg atatgcagga aaccatcgac gaaacgctgg
    21961 atttcttcct gcgcaccgtt gatcttacgg ataaaccatc atgaccaaag taggcttacg
    22021 cattgatgtc gatacctttc gtggcacccg tgaaggcgtg ccgcgtctgc tggaaatctt
    22081 gagtaagcat aatattcagg ccagcatttt tttcagcgtc ggcccggaca atatgggccg
    22141 ccatctctgg cgactggtga agccacagtt tttgtggaag atgctgcgct caaacgcggc
    22201 atcgctttat ggctgggata ttttactggc aggtacggcc tggccaggta aagagattgg
    22261 tcatgccaat gccgatatca ttcgtgaagc ggctaaacat cacgaagtcg gtctgcacgc
    22321 ctgggatcac catgcctggc aagcccgtag cggtaactgg gatcggcaaa caatgatcga
    22381 cgatattgca cgcggtcttc gcactctgga agagattatc ggtcaaccgg taacctgttc
    22441 tgccgctgcg ggctggcgtg ccgaccagaa ggtgatcgaa gcaaaagaag cgttccattt
    22501 gcgctacaac agcgattgtc gtggggccat accgttccgt ccactgcttg aatcaggaaa
    22561 ccctggcact gcgcaaattc cggtgacctt acccacctgg gatgaagtga ttggtcggga
    22621 tgtgaaagca gaagatttta acggttggtt actcaaccgc atcctgcgag ataaaggcac
    22681 gccggtttat accattcatg cagaagttga aggctgcgct tatcagcata attttgtgga
    22741 tctcctcaaa cgcgcagctc aggaaggcgt gacattttgc cctttaagcg aactgttatc
    22801 agagacgttg ccgctcggac aagttgttcg cggaaatatt gccggacgtg aaggctggct
    22861 gggttgccaa caaattgcgg gtagtcgctg atgaaatcgg tacgttacct tatcggcatc
    22921 ttcgcgttta ttgcctgcta ttacctgtta ccgatcagca cgcgtctgct ctggcaaccc
    22981 gatgaaacgc gttatgcgga aatcagtcga gaaatgctgg catccggcga ctggattgtg
    23041 ccccatctgt tagggctacg ttatttcgaa aaacccattg ccggatactg gattaacagc
    23101 attgggcaat ggctatttgg cgcgaataac tttggtgtgc gggcaggcgt tatctttgcg
    23161 accctgttaa ctgccgcgct ggtgacctgg tttactctgc gcttatggcg cgataaacgt
    23221 ctggctctac tcgccacagt aatttatctc tcattgttta ttgtctatgc catcggcact
    23281 tatgccgtgc tcgatccgtt tattgcattc tggctggtgg cgggaatgtg cagcttctgg
    23341 ctggcaatgc aggcacagac gtggaaaggc aaaagcgcag gatttttact gctgggaatc
    23401 acctgcggca tgggggtgat gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc
    23461 gtgctgccat gggtagcgac gcaaaaacgc tggaaagatc tctttattta cggctggctg
    23521 gcggttatca gttgcgtact gacggttctc ccctggggac tggcgatagc gcagcgggag
    23581 cctgacttct ggcactattt tttctgggtt gagcatattc aacgctttgc actagatgat
    23641 gcccaacata gagctccgtt ctggtactac gtgccggtca tcattgccgg tagcctgccg
    23701 tggctgggat tactccccgg tgcactgtac acaggctgga aaaaccgcaa gcattccgca
    23761 accgtctatt tgttgagctg gacgataatg ccgctgctgt ttttctccgt cgctaaaggc
    23821 aaattgccca cctatattct ttcttgcttt gcacctctgg caatgctgtt ggcgcattac
    23881 gcgttgctgg cagcaaaaaa taatcctctg gcgctgcgga ttaatggctg gattaacatc
    23941 gcttttggcg tcactggcat tattgccaca tttgtgatct ccccctgggg accaatgaac
    24001 acgccggtgt ggcaaacctt cgagagctat aaagtctttt gtgcctggtc gattttttcg
    24061 ctatgggcat ttttcggctg gtacacctta acaaacgtcg aaaagacctg gtctttcgcc
    24121 gcgctttgcc cgctggggct ggcgttgctg gtaggatttt caattcctga cagagtcatg
    24181 gaaggaaaac atccgcaatt ttttgtcgag atgacacaag aatcacttca gccaagccgc
    24241 tatattctta ctgatagcgt cggtgttgcc gcaggtctgg catggagcct gcaacgcgat
    24301 gacatcatca tgtatcgcca gacaggtgag ttgaaatacg gccttaatta tccggatgcg
    24361 aaagggagat ttgtcagcgg tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag
    24421 gggattatta cactcgtgct ttcggttgac cgcgatgaag atatcaacag tctcgccatt
    24481 ccgcccgcag atgccatcgt tcgtcaggag cgtctggtgc tgattcagta tcgtcccaaa
    24541 tgatctggct aacattagtc tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga
    24601 aacaggcaac ctgctttgtg gcgataaaca aacggcgcaa acatatcgtg ctgtggctgg
    24661 gactggcgct ggcttgtctt ggtcttgcca tgatgctctg gctgctggtc ttgcagaacg
    24721 taccggtagg cattgcctac ccgatgttaa gtctgaattt tgtctgggtg acgctggctg
    24781 cagtaaaact gtggcacgaa ccggtatcgc cgcgtcactg gtgtggggtg gcgttcatta
    24841 ttggtggcat tgtgatcctc gggagtacgg tgtaatgggc ctgatatggg gattattcag
    24901 cgtcattatt gcctcagttg cgcaattaag cctgggtttt gcggcaagtc atctgccgcc
    24961 gatgacgcac ttatgggatt ttatcgcggc tctgttagct tttggcctgg atgcccggat
    25021 actgttactc gggctgctgg gttatctgct ttccgttttt tgttggtata aaacgctaca
    25081 taaacttgcg ctaagcaaag cctatgcctt gctcagtatg agttatgtgc tggtatggat
    25141 tgcttcaatg gtacttcccg gctgggaagg gactttttcg ctgaaagcac tactgggagt
    25201 agcctgtatt atgagcgggt tgatgctgat tttcctgccc acgacaaaac aacgttactg
    25261 agttttccct gccgctgtac actgccgttc ccactcatcg gggctataac tgcttgccga
    25321 gaggactttc accgtatgca gcttttcacg attaatacaa taaagcgcat tctgcaaagg
    25381 cgagagtaaa tcccccactt tcacggcgaa gtcactcttc acttcggcga tcatttttat
    25441 cgcgccgcca gcatcgcaga gcataagtac atgtctgtta tcttgtttat tgcagcacga
    25501 ttttttgtgc cagccattcc attgcattat cctgtttgct aagagttttc acatcaattt
    25561 acagattaac agataaactc tttatcaacg gcaatggaat ggcagaaatg agaagtgaaa
    25621 cggagggtta attattgctg acgttgcacc cactctttta gcgcctgacg tgaaatttta
    25681 ataccgccgt ttttcagctc cggcggtaaa gtcagccagc gcaccggttg ttgaaaacga
    25741 gccagcttat ctttcaccca ttcgccaaga tcaacggttt gctggtcata ctccaccacc
    25801 gctaccggcc gatgaccaaa ctccttgtcg gcaacggaaa cgataaacac ctgcaaaacc
    25861 gcaggatgtg cagcaataac acgctcgact tcttccggct gaataccctc accgccactg
    25921 aagaacagat tgtctaaacg tccgacaatg gtcagcttgc cattatgcat ctcaccgcga
    25981 tcgcgcgtag cgtaccagcc ttcgtcatta accagtgaaa ccagttgccc gttacgccag
    26041 taaccttctg ccatactggc agcccgcagc cacacttcat tattaacgat tttcacttcc
    26101 cgacccggca gcggcaaacc aacgtctgcc aggccgtcgg cttctttcgc acataccgtg
    26161 gaggcaaact cggtcagacc atagccgcaa aagcaacgaa tcccctgctc gcgcgcctgt
    26221 tccgtcaact cgaccgggat agccgcgccg ccaagtaaca ccgctttcag ggaaacggaa
    26281 ctgcggttaa ccggcaaacg ccagagttgt gttggcacca gtgaagcgtg agtacagcct
    26341 gccagcattt gctccaatgg ctgtttatca cgcaccgtca tccgcgcacc agcgtataac
    26401 cagcgccaca taattccctg accggagacg tgaaacagcg gtaaagagag caaccagtca
    26461 tcgtgatcgc caaacggaat cagcgataac acaccttgcg cactggcaag atgggcctga
    26521 taagtatgga cagcggcttt cggcaaaccg gtagaacccg aggtcaacgt cattgagcac
    26581 agacgcgtcg gctgccacgc agcggcatgt acgccttcaa ccagctgaat gtgcagcgac
    26641 gttaatgccg gaaacgtgtt ttccccatcc ggcaccagcg caaattgcag cgtcagattg
    26701 ggcagcaatt cttcaagcaa cggttgcggc agctgagggt tcacgggcaa cacccgcgcc
    26761 ccgcattgca gtaacgccag ccaggcgagc agcgtttgcg gcgtattcca cgcccgcaac
    26821 atcacgccgc tgccctcaac caccccctgc gccgcaaagc ccgtagctaa ctcatcgacg
    26881 cgagcacaaa gctcgcgcca gttgagttgc tcgtcattaa gacgtaaggc gatggcttct
    26941 ccccgcactt gccgccagtg acgccacggc cagtcagaga agatcataac aactgctcct
    27001 gtacatcagc gtccactaac ggcagtgggc tccccggcca gcgacgaacc tgctgcgcct
    27061 gcatcagatc cagcgtgtcc agtcccggaa tggtgtctgg cgttaaccag gcagcaatcc
    27121 gcgccagttg cgttagacct aagctcgact caatggaaga actgatcacc gccgtcagcc
    27181 ccagcgcatg cgccgcctgt acctgctcgc gtactttttc cagactgccc gtgagcgtgg
    27241 gtttgataac taccgcgcgc acgccctctt cagccacaaa ggcaaaatcc ggctcgcgca
    27301 ggctttcatc ccaggcaatg gcaatgccgg tttcacgggc aaacgctcgc gaatcatcgc
    27361 gggttttgca cggctcttcg agaaacgcga tgcggtggcg ataatccggg ttaacatatt
    27421 tggcaaactg ctgacctttc agcggtgtcc aggcgcgatt tgcgtcaaga cgcaaatgca
    27481 gatccggaat tgcctccagc aacagattca ccaccatgcc gtcgcgcacc gcttcgtaca
    27541 atcccacttt gaccttcgcc actttctcgc ccggcatatc ggcaagtttg aggatcagat
    27601 cgtccggatc gccattacac agcggtgccg cacggtagtt ggctgcttgc ggcaacgtat
    27661 ctgtcagttc tgccaatgca cagcttacgc caaaggccac ggaaggcatc tgcggtagct
    27721 cgcaatcgcc tgccagccag ttatttaccc aggcaagcag cacgctttgc gcctcttccc
    27781 agttttcctg gctgaagccc ggcagtgggg agatctcccc ccacccttcg cgctcgccct
    27841 cacgcaggca aacgtacagc ccgtcgcggg tttttaaccg cctgtcgcgc agaaccaccc
    27901 ccgcgtccat ggggatctgc cagcggtata cctgcgcgct acgcattacg gattccgttt
    27961 aaatttgctg aaatcaggct gacgtttctg gttgaaagcg ttgcgacctt cctgaccttc
    28021 ttccgtcatg tagaacagca tggtggcgtt gcccgccagc tcctgcagcc ccgcctgccc
    28081 gtcacagtcg gcgttcagcg cagctttcag gcagcgcagc gccatcgggc tgttttgcag
    28141 catttcgcgg caccaacgga cggtttcttt ttccagatcc gccagcggta ccacggtgtt
    28201 gacaaggccc atatccagcg cctgttttgc gtcgtactga cggcacagga accagatttc
    28261 tcgcgctttt ttctgcccga cgatacgagc catgtaggaa gcgccccagc cgccgtcgaa
    28321 ggaaccgact ttcgggccag tctgaccgaa gatggcatta tctgctgcga tagtcaggtc
    28381 gcacatcatg tgcagaacgt gaccaccgcc gatggagtag ccagccacca tcgcgacaac
    28441 cggtttcgga caggtacgga tctgacgctg gaagtccagc acgttcaggt gatgtacgcc
    28501 ggaatcatct ttatagccgc cgtaatcacc acgcactttc tggtcaccac cggagcagaa
    28561 cgctttatcg cctgcgccag tcagaatgat cacgccgata ttgtcgtcat aacgcgcatc
    28621 tgccagtgcc tggatcatct cttttaccgt cagaggacgg aaggcattgc gcacctgcgg
    28681 acgattaatg gtgatttttg cgataccgtc ggtggatttt tcataacgaa tgtcctcgaa
    28741 accttcggag cagtcgtgcc attcaaccgg tgcgtaaggc attgcttcat caggataaat
    28801 catattgtgt cctttagtca gaaacgcaag atctgcgcca gacttgcgat tacgccagcg
    28861 ggattttccc gatgcgcgtt atgtccggcg cgaggaatga catggcagtc ggcagccagt
    28921 tccgccgcca gggcgcggaa tttgctgtca cgttcaccac ataaataata aaacgcaaat
    28981 gtgcgggcgc taaggttagc acgtaaatca ggctggacgg cgagagaagt cgcctccagc
    29041 atggcggcaa gggttgcgcc attattgttg ctgcgcagcg ccaccagctc ccggcgttga
    29101 tcgtcattga gcgaggcaaa aacaggctgt tgataccagt cggcaaatac cgccgttaac
    29161 ggttctgtgc gaaaacgctg cgcccattgg cgatcggaac gctgacgttc cgcacgttgt
    29221 tcagcatttt gcagccccgg atgcccgcct tcgacaataa ccccacaaag ccccgccagc
    29281 ccctggcaag ccgccatcat cgccacccgt ccgccaagcg agtaccccac cagccagaag
    29341 tcaaggatgt tgtaactaac caaggtttta cgcagtaagt cggtgacatc atcaaatcca
    29401 tcgacgctaa tcgccgccga accaccatga cctaggagat caacatacaa ccgtgagtag
    29461 tcggcaaacg cctcgcccac ttcttgccat tcgtggcaat cgccggaaaa accgtgcaaa
    29521 aacaccagcc agggtaaacc tagttttccg tgttttgcct gcgcgtgcag gatcataaat
    29581 ggcttacctg cgccagaagt tgctggagcg tttgcgcacc atcggtgtcg ttaaccacca
    29641 tttcaatcac cgtggtggtt ggcgtgcgcc aggcgtcggc aaatgccgtt tcaagttcct
    29701 gccagttttg cggatgatga tatttcagct cgaacatcgc ggcggcgtgc tcaaaatgga
    29761 cgttttgcgg catcagatag aaacgctcac gctcgctttg cggcgttggc aacagcgaga
    29821 aaatttgccc gccgttgttg ttcaccacaa ttaataccag cggcgcagaa acctgacgca
    29881 ataacgccag cgcgttgaga tcgtaaagcg cggagagatc gcccacaatc gccagcgtcg
    29941 gtttgccgct tgcccgctga acgcctgcgg cggtcgaaag cagtccgtcg ataccgctgg
    30001 cccctcggtt gctgtacacc gggtaacctg ccggaagttg cgaaagcgca tcaatcagac
    30061 gtaccaccag gctgttacca acaaacaatt gcccctgttc aggcagatag tcgctgatgc
    30121 gatgcgccag ttgcgcttcg ccaaacgcat cacggcgggc aataaccgcc tgcattgcct
    30181 gttccgccag gcgcgggatt tcaacgcacc agggctggcg tttttctgcc ggatgcagct
    30241 ccagccagtc ggcaatattg gcaattaagc gacgtccgcg atggtgtgcc ggatcaagtc
    30301 gcccttcaat gtcatcaaca atccagtact cttctggttc acagcttgcc tgccattgca
    30361 ggagccgttt ccccgtcagg ctacttccca gttgcaccac aatttgcgcc tgctgcagct
    30421 cgctggtcgc tttggcattg cctaaccaaa gatcggcaca cggcagcggc tgcccggttt
    30481 gtgacagcac atcgccaatc agcggccagc caagagtttg cgcccacagg gcaacttttt
    30541 tgccctcttc cgcactcatg cgcccggcaa ccaccacgcc gcgcttttgt cgccagaaga
    30601 accagtcgcg ctgtttttca ctttccagac gaggcgcttc acgcagccac ggtttgtcgt
    30661 cctgccacca gtcacccaga cgctgttgcc agctaagccc ggtatcgtcc atttcgccat
    30721 acagcggttc agcaaacggg cagttgatat ggactccccc cgcatgaagc gtaccgagag
    30781 cgtggtcgat ggtagaaacc agccaacgtg cggggatatc ctgggtcggg cgcggcaatg
    30841 aaatactgtg cgtggggtga gaggcgaaca ttcccggctg gcgaattgcc tgattcgcgc
    30901 cgcagtcaat tagctccggc gggcgatcgg cggttaagag aatcagtttt tctccggtta
    30961 acccggcttc aatcagtgcc ggatagagat ttgccaccgc cgtgccggag gtcacaatca
    31021 ccgccaccgg ctgcttgctg actttcgcca gccccagcgc cagatgcccc aacccacgct
    31081 catcgaaatg ggtgtggtga atgaatgcgg aattctccgc cgccgctaac gttaacggtg
    31141 tagaacgcga gcctggggcg atacagatgt gtctgacgcc gtgacgcgtt aatgcttcca
    31201 gaatgaccgc cgcccagcgt cggttaaatg cgcttactga catgagtttg tccggtatca
    31261 atattgcggc taagtataag gagctacaaa aatagattat tgatatgaat cggtaatgat
    31321 gcgactcatt actattccat ttgtaataaa gtacgcagcc ctgccgcttt gttgtcgatt
    31381 tcctgccact cttgctcggg gtcggaacca cggacaatgc ccgcgccagc atataatcgc
    31441 acgacattgc cgctaatttt tgctgagcgc agggaaacgc agaattcgct ttgttgtaat
    31501 gagagatagc ccgcagaacc ggcgtaccat tctcgggtga acggttcgtg acgggcgata
    31561 aactgtcgcg ccagatcgcg cggtaagcca gcaactgctg ccgttggctg caactgatgt
    31621 aaacagatca catcatccgc tttgttgagt gaagtccaga tacagcggcg aagatgctgc
    31681 actttacgca gacgcagtac ctgcggcggt aaaacatcca gcgtctgggt atcggcctgt
    31741 aatcgttggc agatatcttc caccaccagc atgttctcgc gctggttttt atcatccgcc
    31801 atcagccact ctcctaactg ctgcgcctgc ttatcatcag gatgatttgc tactgttccc
    31861 gccagcgctt cagtacgcag cgctttgtca cgccgccgcc ataaccgttc cggtgaagag
    31921 ccaagaaaag cattttcgcc atcaaaggcc atgtaaaaat ggtagcaatt cagattcagt
    31981 cgacgactgg cagccatcat cgccgccgcg ttgaccggac ttgcgaaatg caggtcagtt
    32041 gcccgagcga gcaccacttt gtcgagctca ccttcggcga tggttttcgt tgccagttcg
    32101 attaattgcg tccagcccgt tttgtccggc cagtgttgtt ctcgcgtggt ggttaaatgt
    32161 aacccaggca agggcttgat actcaccagt gtggcgataa attcttttgc ctgaatcgca
    32221 tcgtgctgaa gggagctttc gctgaatagc gtcagccgca gcgtggcttt accgccacag
    32281 cgtcgccatt ccaggcgggg taaaagtaaa ttgccctgcg acgggtcaaa agcattcagc
    32341 ccccaaatgc gtaagtcagc gtgttccggg tgctggcgaa gaaaacgttg tgcctggtcc
    32401 aacgacgtaa aacgggtaat cgcgcccagg acggcagctt cttcatcacc attacgttgt
    32461 tgccagtaga attgcgggta cgtttgctga ctggccagcc agctcaaggc atcaaaagcg
    32521 tctttgagag ggaaaggaat atcgataacc cgaatgccgg gtgtcgccgg aatctcttgc
    32581 gacaaatggc gcagtagatt ttccagcgcc gtagtaagtg attgcacgcg gacctctccc
    32641 tgttaaaagc ctcacattat acggggtact acaaaaaaaa tgcagtaccc cggtgtaggg
    32701 aggttttaac ggcgtgccag caacagtcct agtaccagcc cgacggccgc gcccacacca
    32761 attccttgcc agggtttttc gtggacgtag tcatcagcac gataaacagc ctgcttcgcc
    32821 cgatagtaat aactgtctga agcctggcta acccgttttt tcacatcatc cagcgctttt
    32881 tctgcatgcg ctttcagctc aacatatttc tgatcggcgg gatcgccaga ggagcggagc
    32941 acctcttcca gtgtttcact aagcagcgtc aggtcgtcat cgatacgtgt atcaccaaac
    33001 tgattagaca ttctcgttct ccatttgcgt aaaacctgtc actaactata gacaagagtt
    33061 gttgattacg cctgaattac ctcgcgtgcc atcccaatgt gtgggatacc atcttcttca
    33121 tagacctccg tcacagggat aaaaccgaaa ctctggtaga agttttgcag atgcgcctgc
    33181 gccccgaggt aaacaggctt atcaggccag tgatgcgtac atgtttcgag tgttttactc
    33241 atcagctgct gccccacttt ttcaccacgc aacgcttcac tgacaatcac ccgacctata
    33301 acgaccggct caagatcatc atcacttttc agaatcctcg catacgccac cagttcatca
    33361 tttttccaac cgagaatatg gcgattatcc cccgtcaaat catcaccatc gatatcctga
    33421 taagggcaat tctgctcgac aacaaacacc gcgcaacgca gttgtaataa ggcataaagc
    33481 tgggaaaccg ataattcaga gtgatgcaga tcttgccatt caatcataat gctctccttt
    33541 tagatgtatg ccttatacta gccggaatat tttttgaaac gtgatgaact catggaatta
    33601 atttttttag gtacttcagc cggtgtgcca acccgcacgc gcaatgtcac ggcaatattg
    33661 cttaacctgc aacatccgac ccagagcgga ctttggttgt ttgactgcgg tgaaggcacc
    33721 cagcatcagc tactgcatac cgcctttaac cctggaaaac tggacaagat ttttatcagt
    33781 caccttcatg gcgatcatct ttttggttta cccggcttgc tgtgcagtcg ttctatgtca
    33841 ggcattatcc aacccttaac gatttatggt ccgcaaggta tccgtgaatt tgtggaaacc
    33901 gcgctgcgga ttagcggctc atggaccgat tatccgctgg aaattgtcga aattggcgct
    33961 ggcgaaattc tcgatgatgg cctgcgcaaa gtaaccgctt atccgctgga acacccactg
    34021 gaatgttatg gctatcgtat tgaagaacat gataaaccgg gtgcattaaa tgcccaggca
    34081 ttaaaagctg ctggcgtgcc gcctggccca ctgtttcagg aattaaaagc gggcaaaaca
    34141 atcacgctgg aagatggaag gcagattaac ggcgcagatt acttagctgc tccagtgcca
    34201 ggtaaagcgc tcgctatttt cggcgatacc ggcccctgcg atgccgcact tgacctggct
    34261 aaaggtgtcg atgtcatggt gcacgaagcg acgctggata taaccatgga agccaaagcc
    34321 aatagtcgcg gccatagctc tacacgccag gctgcgacac tagcccgtga tgctggagtc
    34381 ggcaagctaa tcattaccca cgtcagctcg cgctatgatg acaaaggttg tcaacacctg
    34441 ttacgtgaat gcaggtcaat tttcccggcg actgaactgg cgaatgattt caccgtgttt
    34501 aacgtttaac tctgcttatt attaaaaaca gggtgaaact tgccctgtta tagcaacccg
    34561 cgctacatat atccacccac acaggcatat tgagcgattt ttgatattta ttcatctcct
    34621 tacgcactat attcagagca ataagttgcc tcttttcatc ttgattcagc acaacctttg
    34681 caactaaaag gagcccatga tggttacagt tgtcagcaat tattgtcaat tatctcaaac
    34741 ccaactcagt cagacatttg cagaaaaatt tactgtgacc gaggaattac tgcagtcttt
    34801 aaaaaaaaca gcgttatccg gagatgaaga aagtattgag ttactgcata atattgcgtt
    34861 aggttatgat gaatttggaa aaaaagctgg gtaatgctgc caacttactg atttagtg
//
LOCUS       DAOFMX010000040        34717 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.040, whole genome shotgun sequence.
ACCESSION   DAOFMX010000040 DAOFMX010000000
VERSION     DAOFMX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.040"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..2028
                     /locus_tag="QBA20_002164"
     CDS             <1..2028
                     /locus_tag="QBA20_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HDQ6292695.1"
                     /translation="DFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGST
                     LSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSV
                     ALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPL
                     HPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAISASVFRNDDGGKGDMQQANWS
                     NPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQN
                     NTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDN
                     GGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRYSEQDGDQTSWQLSHTFYNDS
                     FSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSS
                     TLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSD
                     SVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYN
                     YIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSV
                     VKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2025..3113
                     /locus_tag="QBA20_002165"
     CDS             2025..3113
                     /locus_tag="QBA20_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HDQ6292696.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3201..4499)
                     /gene="exuT"
                     /locus_tag="QBA20_002166"
     CDS             complement(3201..4499)
                     /gene="exuT"
                     /locus_tag="QBA20_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDQ6292697.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            4983..6395
                     /gene="uxaC"
                     /locus_tag="QBA20_002167"
     CDS             4983..6395
                     /gene="uxaC"
                     /locus_tag="QBA20_002167"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDQ6292698.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLVQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6410..7897
                     /gene="uxaA"
                     /locus_tag="QBA20_002168"
     CDS             6410..7897
                     /gene="uxaA"
                     /locus_tag="QBA20_002168"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDQ6292699.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            7980..8531
                     /locus_tag="QBA20_002169"
     CDS             7980..8531
                     /locus_tag="QBA20_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDQ6292700.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8536..9780)
                     /gene="sstT"
                     /locus_tag="QBA20_002170"
     CDS             complement(8536..9780)
                     /gene="sstT"
                     /locus_tag="QBA20_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDQ6292701.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10376..11341)
                     /gene="alx"
                     /locus_tag="QBA20_002171"
     CDS             complement(10376..11341)
                     /gene="alx"
                     /locus_tag="QBA20_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDQ6292702.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11612..12610)
                     /locus_tag="QBA20_002172"
     CDS             complement(11612..12610)
                     /locus_tag="QBA20_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDQ6292703.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12689..13381)
                     /locus_tag="QBA20_002173"
     CDS             complement(12689..13381)
                     /locus_tag="QBA20_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDQ6292704.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13458..13961)
                     /locus_tag="QBA20_002174"
     CDS             complement(13458..13961)
                     /locus_tag="QBA20_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDQ6292705.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14046..15182
                     /gene="rlmG"
                     /locus_tag="QBA20_002175"
     CDS             14046..15182
                     /gene="rlmG"
                     /locus_tag="QBA20_002175"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDQ6292706.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15467..15781
                     /gene="higB"
                     /locus_tag="QBA20_002176"
     CDS             15467..15781
                     /gene="higB"
                     /locus_tag="QBA20_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HDQ6292707.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15778..16194
                     /gene="higA"
                     /locus_tag="QBA20_002177"
     CDS             15778..16194
                     /gene="higA"
                     /locus_tag="QBA20_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HDQ6292708.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16239..18257)
                     /gene="fadH"
                     /locus_tag="QBA20_002178"
     CDS             complement(16239..18257)
                     /gene="fadH"
                     /locus_tag="QBA20_002178"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDQ6292709.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGSAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18483..20834)
                     /gene="ygjK"
                     /locus_tag="QBA20_002179"
     CDS             complement(18483..20834)
                     /gene="ygjK"
                     /locus_tag="QBA20_002179"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDQ6292710.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20851..21270)
                     /locus_tag="QBA20_002180"
     CDS             complement(20851..21270)
                     /locus_tag="QBA20_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292711.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21248..21601)
                     /locus_tag="QBA20_002181"
     CDS             complement(21248..21601)
                     /locus_tag="QBA20_002181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292712.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21602..21922)
                     /locus_tag="QBA20_002182"
     CDS             complement(21602..21922)
                     /locus_tag="QBA20_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292713.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIKGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22056..23489)
                     /locus_tag="QBA20_002183"
     CDS             complement(22056..23489)
                     /locus_tag="QBA20_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDQ6292714.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23552..24001)
                     /locus_tag="QBA20_002184"
     CDS             complement(23552..24001)
                     /locus_tag="QBA20_002184"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDQ6292715.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(23998..27084)
                     /gene="ebgA"
                     /locus_tag="QBA20_002185"
     CDS             complement(23998..27084)
                     /gene="ebgA"
                     /locus_tag="QBA20_002185"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDQ6292716.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRMMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27267..28250)
                     /gene="ebgR"
                     /locus_tag="QBA20_002186"
     CDS             complement(27267..28250)
                     /gene="ebgR"
                     /locus_tag="QBA20_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDQ6292717.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28468..28799
                     /locus_tag="QBA20_002187"
                     /pseudo
     CDS             28468..28799
                     /locus_tag="QBA20_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28841..30220)
                     /gene="ygjG"
                     /locus_tag="QBA20_002188"
     CDS             complement(28841..30220)
                     /gene="ygjG"
                     /locus_tag="QBA20_002188"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDQ6292718.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30638..32158
                     /gene="aer"
                     /locus_tag="QBA20_002189"
                     /pseudo
     CDS             30638..32158
                     /gene="aer"
                     /locus_tag="QBA20_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32312..32935)
                     /gene="nfeR"
                     /locus_tag="QBA20_002190"
                     /gene_synonym="yqjI"
     CDS             complement(32312..32935)
                     /gene="nfeR"
                     /locus_tag="QBA20_002190"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDQ6292719.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33212..33957
                     /gene="nfeF"
                     /locus_tag="QBA20_002191"
                     /pseudo
     CDS             33212..33957
                     /gene="nfeF"
                     /locus_tag="QBA20_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34011..34086)
                     /locus_tag="QBA20_002192"
     tRNA            complement(34011..34086)
                     /locus_tag="QBA20_002192"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34051..34053),aa:Ile,seq:cat)
     gene            34211..34717
                     /gene="mug"
                     /locus_tag="QBA20_002193"
                     /pseudo
     CDS             34211..34717
                     /gene="mug"
                     /locus_tag="QBA20_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8462 a         8935 c         9134 g         8186 t
ORIGIN      
        1 gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac actatttaat
       61 gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga tgggatgcgt
      121 atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac cccagtaatg
      181 gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact tttgggatcg
      241 ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc aggcagctac
      301 agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct tgtgccgttt
      361 accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc aggtaaaact
      421 acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg cctgccatta
      481 catccgcaat atgagctcta cgcagggctg gcgaatgccg atgatgtgag tgctttcgag
      541 ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat cagcgccagc
      601 gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg gagtaatccg
      661 ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg tacaaccgac
      721 agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac agtttcactg
      781 aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa cactgatgat
      841 agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca gacaacagcg
      901 caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat gcgtgactgg
      961 attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga taacgacgac
     1021 aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag caataacaac
     1081 agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg cgatcaaacg
     1141 tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga acttggcgta
     1201 accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg ttgggatggg
     1261 caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa tcatgatcat
     1321 ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta tggcgttaat
     1381 ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt gaaaggcttc
     1441 tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc aggcagccga
     1501 actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt tcagtctggt
     1561 tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa catcattaac
     1621 ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg cgaagtgtct
     1681 gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt gaaaaaattc
     1741 ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg atttacactt
     1801 gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt ccttaagtgt
     1861 ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt tacttgtagt
     1921 gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt gaaacagcct
     1981 aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga aatcgattga
     2041 ttgcggcgat attgggcttg tttggcacgc tcactggcgt tcaggcagct cctgacgtga
     2101 ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc tggaaggatg
     2161 aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct gacctccaac
     2221 aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact gaacaacgca
     2281 gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta agtagcgatg
     2341 aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag gattctaata
     2401 gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag tttcttacgc
     2461 tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg aaggggaaag
     2521 ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc ctgaatacgc
     2581 tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg ccaagggtgc
     2641 agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa gatctgacca
     2701 tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat gaaatcatgc
     2761 tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct atctatcgtc
     2821 agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa atgtacaacc
     2881 cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg aacagcatta
     2941 acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg atgtgtgtgc
     3001 cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag gccgggtact
     3061 acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat taaccctgca
     3121 aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg cggcttcgtc
     3181 gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt ttcctgcgcc
     3241 acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc cagcaggtca
     3301 aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag tgcaccgaca
     3361 accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc gttcgccgtc
     3421 gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc agacagtgcc
     3481 tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt ggtgaacaga
     3541 ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat cttacgggaa
     3601 acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc accgaggata
     3661 caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc tttcaggtta
     3721 aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc ccaggccggt
     3781 tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag gatctgacca
     3841 acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt aataatatag
     3901 tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa aatcagccat
     3961 gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg ccagctgtgc
     4021 atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg
     4081 ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga gctggctttc
     4141 agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc aacagctagg
     4201 ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag cacagcaaac
     4261 attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac cggttgcatg
     4321 accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt
     4381 aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc
     4441 acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg
     4501 taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg
     4561 aagatgttag ttacgaccgg gtaaccgaaa aaaaaaccgg cacgccccga acgttgccat
     4621 gaaagtttcg tgctaattcg gcttccgtac cggttaaccc gattcatcta acaaatgaat
     4681 gaatcggcaa tttgcattcc gtcgacttat gatttgcgac ggcagaaaga taacttgtca
     4741 tacaacctta aaaggtgaga gccatcacaa atgtgcgaat atttgtaggg acattacctg
     4801 acgacagcga ggccagtact ggcgcggcct gcagcgagat ttaccacttt gagagtaatt
     4861 tttttaacta cgtttattga tctaactcac gaaaatatct tcggactctg gaaattggtg
     4921 tgataacttt gtcagcatcg caccataagc aagctagctc actcgttgag aggaagacga
     4981 aaatgactcc gtttatgact gaagatttcc tgttagatac cgaatttgcc cgccgtctgt
     5041 atcacgacta cgcaaaagac cagccgattt tcgattacca ttgccatttg ccgccgcagc
     5101 agattgcgga agactatcgt tttaaaaacc tgtatgacat ctggttgaag ggcgatcact
     5161 acaaatggcg cgctatgcgt accaacggtg tggctgagcg tctgtgtact ggtgatgcgt
     5221 ctgaccgtga aaaatttgac gcctgggcgg cgactgttcc gcacactatc ggcaacccgt
     5281 tataccactg gacgcacctc gaactgcgtc gtccgttcgg tatcactggc aaattgcttt
     5341 ctccgtcaac tgccgatgaa atctggaacg aatgtaacga actgctggtg caggataact
     5401 tctccgcgcg cggcatcatg cagcagatga acgtgaaaat ggtcggcacc accgatgacc
     5461 cgatcgattc tctggagcat cacgcagaga tcgccaaaga cggctctttc accatcaaag
     5521 tgctgccgag ctggcgtccg gacaaagcct tcaatatcga acaggcgacc tttaacgact
     5581 acatggcgaa gctgggcgaa gtttccgata ccgacattcg ccgctttgct gacctgcaaa
     5641 ctgccttgac caaacgtctg gatcacttcg ccgctcacgg ctgtaaagtg tctgaccatg
     5701 cgctggacgt ggtgatgttt gctgaagcga acgaagcgga actggacagc attctggcgc
     5761 gccgtctggc tggcgaaacc ctgagcgagc acgaagtggc acagttcaaa actgcggtac
     5821 tggtgttcct tggtgccgaa tatgcacgtc gcggctgggt acagcagtac cacattggcg
     5881 cgctgcgtaa taacaacctg cgtcagttca aactgctggg gccggatgta ggctttgact
     5941 ccatcaacga ccgtccgatg gcggaagagt tgtctaagct gctgagcaag cagaacgaag
     6001 aaaacctgct gccgaaaacc attctctact gcctgaaccc gcgcgataac gaagtgctgg
     6061 gcaccatgat cggtaacttc cagggcgaag gtatgccggg caaaatgcag ttcggttccg
     6121 gctggtggtt taacgatcag aaagacggta tggaacgtca gatgacccaa ctggcgcagc
     6181 tcggtctgct gagccgcttt gtcggtatgc tgactgacag ccgtagcttc ctgtcataca
     6241 cccgtcacga atacttccgc cgcattctgt gccagatgat cggtcgctgg gtggaagcgg
     6301 gcgaagcacc ggcggacatc aacctgctgg gcgagatggt gaaaaatatt tgctttaaca
     6361 atgcgcgtga ctacttcgcc attgaactga actaaggtct ggggttgata tgcaatacat
     6421 caagatccat gcgctggata acgtcgcggt cgctttagca gatttggctg aaggcacaga
     6481 agtcagtgtc gataaccaga ctgttacgct gcgccaggat gttgctcgtg gacataaatt
     6541 tgcgttaacg gatatcgcaa aaggggccaa cgtcatcaaa tatggcctgc cgattggtta
     6601 tgcattggcg gatattgcgg cgggggaaca cgtccacgcc cacaatacgc gcacgaatct
     6661 gagcgatctg gatcagtatc gctatcaacc tgattttcag gatctgcctg cgcaagcggc
     6721 agatcctgaa gtgcagatct atcgtcgcgc taacggcgat gtcggggtgc gtaacgagct
     6781 gtggatcctg ccaaccgtgg gctgtgtcaa cggcatcgcg cggcaaatcc agaaccgttt
     6841 cctgaaagag accaacaacg ccgaaggtac cgacggcgtg ttcctcttca gccacaccta
     6901 tggctgctca caactgggcg acgatcacat caacacccgc accatgctgc aaaacatggt
     6961 gcgccacccg aacgcgggcg cagtgctggt gattggtctg ggctgtgaaa acaaccaggt
     7021 tgccgcattc cgtgaaacgt tgggcgatat cgatcctgaa cgcgttcatt tcatgatctg
     7081 ccaacagcag gatgatgaga tcgaagccgg gatcgagcat ttgcatcagc tgtataacgt
     7141 gatgcgcaac gataaacgcg agccaggcaa actcagcgaa ctgaagtttg gtctggagtg
     7201 cggtggttct gacggtcttt ctggtattac tgctaacccg atgctggggc gtttctctga
     7261 ctacgtgatt gctaacggcg gtactaccgt actgaccgaa gtgccggaga tgtttggcgc
     7321 agagcagttg ctgatggacc attgccgcga cgaagcaacg tttgaaaaac tggtcaccat
     7381 ggtcaacgac ttcaaacagt actttattgc ccatgatcag ccgatctacg aaaacccatc
     7441 accggggaac aaagcgggcg gtatcaccac gctggaagac aaatcacttg gctgtaccca
     7501 gaaagcgggt tccagtgtcg tggttgacgt gctgcgttac ggcgagcgtc tgaaaacgcc
     7561 agggctgaac ttgttaagtg cgccgggtaa cgatgccgta gcgaccagcg ccctggcggg
     7621 tgcgggctgc catatggtgc tgttcagtac tggtcgcggc acgccatacg gtggatttgt
     7681 gccgacggtg aaaatcgcca ccaacagtga actggcggcg aagaaaaaac actggatcga
     7741 ctttgacgcg ggtcagctga tccacggtaa agcgatgccg cagttgctgg aagaatttat
     7801 cgacaccatc gttgagtttg ccaacggtaa gcaaacctgc aacgagcgta acgacttccg
     7861 cgaactggcg atcttcaaaa gcggcgtaac gctataaagt cgaaatgagt tgttaataaa
     7921 aaacggcgtt tcataatgtg aagcgccgtt tgcttttcca tacctgctaa ggatttatca
     7981 tgaccgcgta ttggctggcc cagggcgtgg gtgtcatcgc ctttctgatt ggtatcacaa
     8041 catttttcaa tcgtgacgaa cgtcgcttca aaaagcagct ttcggtctat agcgccgtta
     8101 ttggcgtaca tttttttctt ctgggcacct atcccgctgg tgccagtgcc atccttaatg
     8161 ccattcgtac attgattacc ttacgcacgc gcagcttatg ggtaatggcg atttttattg
     8221 tgctgactgg cggaattggc ctcgcgaagt tccatcatcc tgtcgaacta ttgccggtta
     8281 tcggtacgat tgtcagtacc tgggcgctgt tccgctgtaa agggctcacc atgcgctgcg
     8341 tgatgtggtt ttcaacgtgt tgctgggtga ttcacaactt ctgggcgggg tcgataggcg
     8401 gcacgatgat tgagggtagt tttctgctta tgaatggcct gaatatcatt cgtttctggc
     8461 ggatgcagaa aaggggaatt gatccgttta aagtagagaa aacccctccc gccatagacg
     8521 aaaggggtta aacaattaat tacgcagggc gctatttgcc agacgatcgt cttccgcctg
     8581 gcaagctgcc gcagtgaaca gcacgtcagt tgaagagttc agcgcggttt cgcaagagtc
     8641 ctgcaatacg ccgataataa agccgacggc aaccacctgc atggcgatat cgttcgaaat
     8701 accgaacata ttacaggcca gtgggatcag cagcaaagaa ccccccgcca cgccggatgc
     8761 gccacaggca cacagagaag ccactacgct caacagcagc gctgtgggca gatcgaccgg
     8821 aatacccagc gtattaaccg cagccagcgt caacacggta atggtgattg ctgcgcccgc
     8881 catattgatg gtggctccca gcggaataga aactgaatag gtatcgcgat ccagattcag
     8941 cttttcacac agcgccatat tcaccggaat gttcgctgca gagctgcggg tgaagaaggc
     9001 atacacaccg ctttcgcgca ggcacagcag caccagcggg aacgggttac gacgaatttt
     9061 ccaccacacc agcaatgggt taaccaccag cgccaccagt aacatacagc caaccagcac
     9121 gaccagcagt tgcgcgtagc cccacagtgt ggagaaaccg gtggttgcca gggtagaaga
     9181 aaccagccca aaaataccga acggtgcgaa gcgaatgacc agtttcacca taaaggtaac
     9241 ggcattcgac atatcattga ccaggttttt ggtggtctcg ttaccgtgac gcagtgcgaa
     9301 gccgaggccg atcgcccaca ccagaatccc gatgtagtta cctttcagca gggcgtcgat
     9361 ggggttggaa accatgctca ttaccagtcc gcgcatcact tcgacaatgc ctgacggcgg
     9421 cgaaatatca cccgcgctac tggacaagtg cagggtagaa gggaaggcaa agctgaagac
     9481 tactgcggcc agagctgcag agaaggtgcc cagcagatag aggaacaaaa taggacggat
     9541 attggttttc tgcccgtgct ggtggttagc aatagatgcc attaccagca tcaacaccag
     9601 gatgggggca acagctttca gtgcgccgac gaacaaagta cctaacagac caacagcttc
     9661 cgccgcgggt tttgagatcc atgccagaag aatccccaga acaaggccga ccaggatttg
     9721 ttttaccagg ctgccatgag ccagacgccg gaatagcccc ggtgaacgtt gcgtagtcat
     9781 ttttcgatcc tttcattgtg ttgtcgcaca tccctggtgc gttctgtcga cgcatctttc
     9841 aggatgtaac aaagtgtttg caaggtcgag tataaggaaa gttacagctt cgggaagcga
     9901 aaaatgctgg attttaaaga gaggtcatgt tttgcaactt aatggttgca tttatttaac
     9961 ataaacagag ggaaggactg gctactaagg ctgattagat aacatatggg caaaactatt
    10021 tggaatatga gaacggttgc gctcctttaa cgtgttatgc catataaagt cactggcgca
    10081 gggagcgcgc agggggcggc caatcgccgc cgccccctgc acccccaggc tctggcgaac
    10141 aaaatcgccg ctgcgcggtt ccctcagcct atcccttgcg gctaccgggt cgggcgcaag
    10201 gtaacgtccc tgtaagatgc gccctcgccc cggcctccag ggaacgcttc ggcgattttg
    10261 acgccaccaa cacccgtgcg gtctttttgt aaagagctat aagccgatta cataattaat
    10321 agctcacctt tgtctggaat gagttatctc taggaatggc ttaatataca gcgaatcatt
    10381 ctccccgctg cttatcatgc cgataattca cccaggcgtt gataataaac gtcatcacca
    10441 gaatgccaaa caccacgccc agcgagacgg cgattggaat atggtagaag tcgacaatca
    10501 gcatcttgat accgataaac accagaatca ccgccaggcc atatttgagc atcgagaaac
    10561 gctctgctac gcccgccagc aggaaataca tcgcacgcag gccgaggatt gcaaacaggt
    10621 ttgaggtcag cacaatgaac gggtcagtgg tcacggcgaa gatagccgga atgctatcca
    10681 cggcaaaaat cacgtcgctc aattccacca gaatcagtac cagcatcagc ggtgtggcgt
    10741 acagcaatcc attcttacgc acaaagaaat gctcgttgtc gatggtgtcg gtcatgcgca
    10801 aatgaccgcg tagccagcgc accagcggct tgtcgccaat gcctgattca tcttcatggg
    10861 cgagcgccat cttcacgccg gtaaacagca ggaaggcacc gaagatatac aggatccagt
    10921 cgaactgcga aatcagccag ctgcctgtga agatcatgat ggtacgcaga acaatcgccc
    10981 cgagcacgcc atacaccagc acgcgacgtt gtaatgccgc cggaacggag aaatagctga
    11041 acaacatcag ccagacaaag acgttatcga ccgccagcga tttctcaatc agataacccg
    11101 tgagaaaggc cagggcctgt ggatcggcaa cggcgcgacc ttcggtttgc accagatacc
    11161 accagaaagc ggcgttaaac agtaacgaca gcgtcaccca gaccagcgac caggccgcag
    11221 cctgtttcat ggtcatggca tgtgccccac gacgcccctg caacaacagg tcgatagcca
    11281 gcataatggc gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg acagtattca
    11341 tagaagttcc ttacacataa aaaaacggcc aacgtcggaa gacgtcagcc gctgcttttt
    11401 atgcatagac ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa tacgtctgcg
    11461 tattcctttc gggttgcccg gtgaccggat gtggtttttc acacatcgta atgacgatcg
    11521 accggcaatg aagttactcc cctttgcagg taacaaagta tgacagacca ttcagtcagt
    11581 caatggtctg ttacatacct tttacactgt tttactttgg caagtatagt tttacgctat
    11641 ctgccggaaa aatcacccca gtttggcggc ggatctcggt cagcagtttg gctgtgatgc
    11701 gactgaccgc cagccctgga tgatccacca gatgctcatc caccagggta gcgaacagct
    11761 ctgcttcgta gagcatggta ttaatatgct gcggctgggt gagatcctgc atctggctgc
    11821 cacgcggcac aaagcagact ttctggcatt cagacagttt ttcaatcacc agcgatcccg
    11881 cttcgccctg aatctcgctt cccaggacag aatcactgac ctttgagtgc tgcaaggtga
    11941 cgctgaaatc accgtaatcc atcaccacca cgccgtgggc gtcaacgccg cttgccagca
    12001 aactcgccgt tgcctgcacg cttttcggct caccaaataa cgccaccgcc gacgccaggc
    12061 agtaaaagcc gatatccata attgaaccgt tagagaatgc cggattaaag gtgttgggat
    12121 tctcaccgtc caggtaacgt tgataccgcg aggaatactg gcaatagttg aaaaagactt
    12181 tacgcagttt gcctgctttc ggcagcgcct ggcgcaacaa atgaaagttc ggcaggcagg
    12241 cggttttaaa tgcctcaaac agcaccacct gattttcgcg cgcacaggca atggcggcat
    12301 ccacttccgc cagattcgac gccagcggtt tttcgcaaat cacatgaatt ttatggctaa
    12361 ggaaaagttg tgtctgggaa aagtgcaggg aattcgggct ggcaatatac accgcgtcaa
    12421 tggcatcgct ttccgccatc gcttccagcg acgtaaacag atgctcgaca gaaaaatcat
    12481 tggcgaagtg ctgagcctgc tcaaggctgc gggaatatac ggcggttaac ttgtatttac
    12541 cgctctcatg ggcggcctcg acgaactggc gagtgatcca gttcgtacca atcacagcga
    12601 aacgtatcat aacgcgtgga tactccataa agggattctg tcgtcaatgt agcacgcctt
    12661 tttcatcttc gacgtgcgca gatacgcatt attctttcgc cggacagcca tgctcactaa
    12721 acggcccaat catgaccgat ggcccgagat atttcggctg gcgatggagg atttttgcgt
    12781 caatcaccgc gctgacacga gcgagtttct cccgcagttg agtataaaaa ccgtgcttca
    12841 tattgaggtc tggcgcgtta aacccgatag cgtcgatgcc gtactgtttt gccagccaga
    12901 tggcgcgttg gttatgaaat tcttgcgaga taatggtgat atggttttcc ccaaacacct
    12961 ttttggcacg cactaccgaa tccagcgttg agaatccggc atagtcgcaa aagatcactt
    13021 tcgccggtac gcctttagcg atcaaagcct gctgcatacc tgatgcttca tcgtaatttt
    13081 tacgtccgtt atcgccactc accagtagcc atttcacttt tccggcgtgg tacaacgctg
    13141 ctgcggtatc aatacgtcgg gtaaagtagc gattacccgg cctcgccccc agcaataaac
    13201 ccacgttacg cgccgggacg gcgttgacat cgccccaggt cagctgcttg ctggcgttga
    13261 ccataacgcg atcggcgata aaaatggtcg ctcctgccac aagtaacagt aagcaggcaa
    13321 tctttagagt ccggcgagaa aaacaaatac ggagaagaag gcgggcaaat gcgcgcagca
    13381 tggtcgttat ccttaacgaa aggggggagg tcgattttag gcgctaaaga aagaaaagat
    13441 aacgcctgac atcattttca gattttgtcc tgcccaagcg acaactgcgt tagccacagc
    13501 cgggtgtcga actcaagctg gtggtactgc ggctccatat ggcagcacaa ttgatagaac
    13561 gctttgttat gctctttctc cttcaggtgc gccagctcgt gcaccacgat catgcgcaaa
    13621 aacggttccg gcgcgttgcg aaacacggta gcgacgcgga tctctgcttt tgctttcagc
    13681 ttgccgccct gcacacgaga aacagcggta tgtaacccga gcgcgttttt tagcacatgg
    13741 atcttattgt catacatcac tttattgatc ggcggggcat tacgcagaaa ctgatttttc
    13801 agatcctgag tatattgcca gagagcttta tcggtggcgt agtcgtgtgt tcccggatag
    13861 cgttttgcca gcacatcgcc cagacgctgt tcgttaatca gtgttcgcac ctgcgaaagc
    13921 agctgctcgg gataaccctg aagataagta agattgctca taaccgccca cgaaaaagag
    13981 aaaaagggta tactcacgca cccttttcag gggcaacgtc gaaattttat cattcaggag
    14041 ggccgatgag ccacttagac aacggtttcc gttcactgac actacaacgt tttccggcga
    14101 cggatgacgt taacccgcta caggcgtggg aagcggcaga tgaatatttg ctgcaacagt
    14161 tggacgacac agaaatcccc ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa
    14221 gctgcgcgct ggcggaacat aagccgtaca gcattggcga ctcatacatc agtgaactgg
    14281 cgacgcgcga gaatttacgc ctaaacggga ttgatgagtc gagcgtgaag tttctcgaca
    14341 gcaccgccga ctacccgcaa cagccgggtg tggtgctgat caaagtgccg aaaacactgg
    14401 cattgctgga acagcaactg cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta
    14461 ttgccggtgc caaagcccgt gacattcaca cttccacgct ggaactgttc gaaaaagtgc
    14521 tcggcccgac caccaccacg ctggcatgga aaaaagcgcg cctgattaat tgcacgttca
    14581 atgaaccgcc gctggccgat gcgccgcaga ccgttagctg gaagctggaa ggtactgact
    14641 ggactatcca taaccatgcg aatgtcttct cccgcaccgg gcttgatatc ggcgcgcgct
    14701 tctttatgca acatctgcca gagaatctcg aaggggagat tgtcgatctc ggttgcggta
    14761 atggcgttat tggtctgacg ctgcttgata aaaacccgca ggcgaaagtg gtgtttgtcg
    14821 atgaatcgcc gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac atgccagagg
    14881 cgttggatcg cagcgagttt atgatcaaca acgcgctctc cggcgtggag cctttccgct
    14941 ttaatgctgt gctctgcaac cctccgtttc accaacaaca tgcgctgacc gataacgtcg
    15001 cctgggagat gttccactac gcccgccgct gcctgaaaat caacggcgag ctgtatatcg
    15061 ttgccaaccg ccatctggat tacttccata aactgaagaa aattttcggc aactgcacca
    15121 ctattgcgac gaataataaa tttgtggtgc tgaaagcggt gaagctggga cgtcgtcggt
    15181 aagattggga gttacctgcc ggatgcggcg taaacgcctt atccggccta cgttcagcac
    15241 gttaccccca ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc tgctcagttc
    15301 tggaatcgcg cttccataaa acaaaaaatc cctgtcacca tcctctacat tctcttgttt
    15361 agcgtttttc tacgtttatt cttccgtcac acagataaat tcatccgttg ccaatctgtc
    15421 aacggatgtt atcatattgc caatttgtca acggacgtga tgaagaatgc acctgataac
    15481 tcaaaaagca ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg agttggtggc
    15541 tctggggaac acgattgcta agggatattt caaaaaacct gagtcattaa aagcagtatt
    15601 cccatctctg gataacttca aatatctgga taagcattat gttttcaatg ttgggggcaa
    15661 tgaattacgt gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt
    15721 tatgacgcat aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg
    15781 attgcgattg ccgacatctt gcaagcagga gaaaagctaa ctgctgtggc accttttctg
    15841 gcgggtattc agaacgagga acaatacacc caggcgctgg aactggtaga tcatctgctg
    15901 ctcaacgatc ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa
    15961 gaatcagcgc ccgaatttgc ggaatttaat gccatggctc aagccatgcc tggcggtata
    16021 gccgtgattc gtacccttat ggatcaatat ggtttaaccc tttccgatct gccggaaatt
    16081 ggcagtaaat ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac
    16141 gctaaaaaat tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac
    16201 gtgccggatg cgttgcatcc ggcgcttcaa ataacgattt aaatctccag cgccagccgt
    16261 gttccctggg caattgcccg tcgtgcgtcc agctccatag ccacatcgca gccgccaatt
    16321 aaatgcacag ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc
    16381 cctgcgcaga tcaccacatt gtccactgct aatacctgcg tttcgccgtt gatcaccaca
    16441 tgcagcccgt catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc
    16501 gagagcagga tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcccggtttg
    16561 ctggctttgc gctggagcat cacaatttgc cgtgggctac gcgggatctg cattccctgc
    16621 gggcttaagc caccagcctg ttgtaggctg ctgtcgatcc cccactcatt acagaacctg
    16681 gcgatattct ggctggtgga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca
    16741 atcccgccac aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca
    16801 tcgagataac tcaatacctt cggatgatcg atcccgtcga taggcggaac acgcggcacg
    16861 atcccactgg cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt cacggtgtga
    16921 ttgagtttca gcgtcacgcc cgtcacttcg atcatccggc ggtagtagcg cagcgtttcg
    16981 taaaactcct ctttgccggg gatctgtttg gcgatattaa actgcccgcc gatctcgctg
    17041 tgcgcatcaa acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc
    17101 ccagcaggtc cagcgccgac caccgccaga ttttttttct gcacggcggg aaggattggc
    17161 attttggttt cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag
    17221 atctgatcga ggcagccctg gttgcagcca atacaagtgt tgatctcatc ggctcgccct
    17281 gattgcgctt ttgacagcag ttccgcatcc gcgagaaacg gtcgcgccat cgatactata
    17341 tcggcatcgc cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg
    17401 gttaccagcg gcagcgagac gtggcctttc agtttgcgcg tgacccagct aaatgcgccg
    17461 cgcggcacgg gcgtggcgat ggtcggaatc cgcgcttcgt gccagcctat tccggtgttg
    17521 ataatggtcg cgcccgccgc ttcaatggcc tgcgccagtt ctaccgtttc ggcaaaagtg
    17581 ccgccgcctt cgaccaggtc gagcatcgac aaccggtaaa taataatgaa gtcgttgccg
    17641 acgcgttcgc gcaccgtacg cactacttct acggcaaatc gcatccggtt gcggtaatcg
    17701 ccgccccact ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac
    17761 ccttcagagc ccatcacttc gacgccatcg tatccggcct cccgcgccag ttgcgcgcag
    17821 cgggcgaaat cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa
    17881 cggttgatgg gggcctgcaa tgcggacggt gcgaccagat gcggttggta gctgtagcgc
    17941 ccggtatgca aaatttgcag ggcgattttg ccgccttcct gatgtacggc ttcggtaatg
    18001 gtgcgatggt gtgggatctg gctggcgtcg ttgagcattg cgctaccttc catgccaacg
    18061 cctgttaaat ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt
    18121 tcggcataaa acgcagccag ccgttcagca ccgtccgggt attcctccag cccggtgtgc
    18181 attgaaccca tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac
    18241 agcgacgggt agctcataaa attgtccagt atgtaaaata attgttatgt agtcggatga
    18301 gttctaattt agccgtcgat ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga
    18361 ttcaaaaaga atacgcccgg tggtaacgcc ggatgcgacg cttgccgtgt tttatccggc
    18421 ttgggggatt gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agccgttgca
    18481 aattactgct tacggaaaaa atcgttatac aacatataca aatgagcggc actccaggag
    18541 aaatttggtg cgccttgctg cgcgcctgtc agcgggttgt aattctcctg aattgggcca
    18601 tcggcggtta atcctttggc gtgcctgaag aacgtatccg ccagcttcag ggcatcatca
    18661 cgataaccgt aacgctccat ccctttcaga ccaaaccaga actgatccac ccatacgcgc
    18721 ccgcgccagt agatatcagc gccaaaagcc ggattggtta acgccgccgt tcccagcgga
    18781 acaaaggtgt tgaactcttt aggatcgagc atcaccttca ccaccgcgtc ggcattggcc
    18841 tgcgttgccg caccgttaaa cagcggcgac cagccttccg gccctttacc gcgctcaaca
    18901 atcggtttgc ccgcgcagcc gttcgccagt ggtttatctt caatacgtac gtcatagtag
    18961 aactgggtag tcgggtcgaa catacaggtg ttgatgtagt ccgcgagctg ctgtgccaac
    19021 tggcgatagc gtttggcctc ttctggctta ccgaggatgg ttgccatctc cgccagataa
    19081 tggttatcgc tgtacatata gctggcctga tccaccgact cctgcaatag cgagtagccc
    19141 agcaacgttc cgtcctgact gcggttttcg gcgaatttca ccgtccagtc gctacgtttg
    19201 ccgccattag cgacatattt atccaactgt tctttgtcga taaaaccaaa gacggcggca
    19261 tcatcacggc ccgattccca cgacgcagcg acctgtgccg gaatttccag actgtcgtac
    19321 tggcctttct ccaccacgcg ggcgtagttg ttcagcccag actgcgtctc ttctttatcg
    19381 ccttttttca ccgtaaacag catctcgccg ctctcggtgt tgtgggcttt gtcgcgggtc
    19441 gcgccatatt ccggcacccc gttgccgtta tgatcgcggt tacgtaacca ccagtcgtga
    19501 taggccacca gtttcgggta catctctgcc aaccaggctt tatcctgggt gacgttgtat
    19561 acttccatca ccgaccaggc ggcaaggctg ggtttggtat tacgttcgtt ccagttgccg
    19621 ccatcgccac cacgctcggg gctaaggttc cacgctatca ggtcggggac aaagcccaca
    19681 tcctgcggac gcacgctgtc gccaggctgg atctgccagg agaagaccgc gcggatattc
    19741 tctttggcga tatccggatt gaaatgcgcc atcgcaaacg cctgcttcca ggtatcccac
    19801 ggccaggtct gattgccgga gaaccagcgt ccggtcaccg acggcgtgac ggtgttataa
    19861 ttcaccgcac cgcccggtga gcgccagtta ccgttgagcg tttcgatcgc tttcaccgcg
    19921 acgcgcgtct gttccggcgt cgcatccgga ttggttaacc ctttcttcag gtattcttcc
    19981 cagcgttgct gcgaggcggt gagataaaac gccggacgcg ccagaatatc gcggatctgc
    20041 atttgctctt tgctaacttc ctgagcggtc agcagatggg aataggtggt gtagagcgtg
    20101 gtcgaaccgt tgatatgcgc cttactggta aagcgattac cgttgatttc agtctgcacc
    20161 ggcagggatt tatgcacctg atattccgat tcgccggagg tcagcagatc ccaggtggcg
    20221 cgcactttgc caaaggtgac tttcaggcca tcacgggtgg cgctgatttt gcgctgatag
    20281 tcagggtatt cgccggcaat ggttttatcg gaaagcggtt tcccttcttt cgcttccagt
    20341 ttttccagca gttcgccatc ccacaccaga tccagcggtt tattgctgat gattttagtt
    20401 tccagtagtg acgtgcgcgg cgtggcgaag cgcagagtca tttcgacctg cacatctttt
    20461 gctgtcagtt tttgcaccag cgcgccggga atactgtatg cctccagcgt gaagttgact
    20521 ttcttgccgt cttgccagac agtcaggcgg tcgaaattgc tggccataaa gttgatgtat
    20581 tcttccgtca gcagcgcaac gcccggaaag ccgcccatgg tgttcgggcc gtctggcaac
    20641 agatgaccat gccaggctcc gagatcgaaa aacggattaa aacgctggtg atcgtcgtaa
    20701 tcgtaatcct tcatgtactg cggcgcacca gtacggttaa tcacgttttt ataattgtcg
    20761 gcgttagcgg catgagcgga aaaacttatc agcagagcgc aggttactgg cgttaaaata
    20821 gttttgattt tcatgagatt acagtcctta ttaccagtag aaatattgca tcaggaagat
    20881 ttggtcgctg ggatcgttag aggcattttt catgtagaag cggctatcaa aagccgtggc
    20941 gaaacgtccg ccgccatatt cgtaccagat accaaactgc atgaaagagg tggtttcatc
    21001 gtcagcgtgg tcagggcgat gtttggcgta ggtgtaagcg gtggagagat aaacgccttt
    21061 taacgcttca tacatggcgc tcaccatcat cccgctttca cttcccggta cgtccagccc
    21121 gtcggcacgt ccggtatggt gccaggcgcg agcggcaaag ttaggggtaa acaggcccgt
    21181 gagatagagt tgtcctgtgc cgtcggtaat ttccagacca ttcatccagg tgatgccttc
    21241 ggcaaggtca tactggatgt aaccgttcat ccattgccgg ataggtgtat ttgtcgtcgt
    21301 agcgatcgta cttgccaaaa tgtagccagg ctttgctttc gtcatgatgt ccggcaggtg
    21361 tggcggtgac gctataacga aagttgccgc ttaagttctg taccttcagc gcggtcataa
    21421 tgtcgcgggt attgggaatg acgtagccca aatcgggtgt gaagtcgccc caccattgca
    21481 ggtcatccag cgaggaatcc tgacgcagac tgagcatgat ttcggtgcca tcattggtac
    21541 gataaccgcc atagaaacgg ttaatcccac cttcgaaacc accccagcta tggtcaggca
    21601 cctaggcatt accgtcgtgg tcggcctgca cggtccagcc ttcgccgtaa atcaggccga
    21661 accaggagcc gtacttcatc tccaggccac ctttaatata ggtgccgtcg ctgtatttat
    21721 gtttattctc accctctaaa tccatatatc caccaagccc taattcgccg taaaaacgaa
    21781 atgaaggaga agatgtcgag gtaattgcgg gtgtttttgt gtcgtcatgt tctgccggac
    21841 gtgcttcttc tcctgccgca gaaaaggcgt agcaaaacgg cagagcaagt aatgctctgc
    21901 atggtgcagt gattaatttc attgtgttaa tccatgagct aaagaaaaag tacagccttt
    21961 tatcgttgta attataaaaa tataaaggca gaaactattt tccctctcct catccaggaa
    22021 gagggaaaat gcctgctcat tttttattag tctgcttaat caacattgct ggctggcgtc
    22081 gcttcaatat ggcgctcttc agccgttaat ccctttatat atttgctgta tttccaccac
    22141 gcaaagccga ggaagataac aataccgccg acgttataaa aaatgatggt cagaatattc
    22201 gcgccagtcg ggaatgtcga agcgacaaac cctacggcaa atatcgcaat cagcattgaa
    22261 acaacgataa taccggtgcg gcgggagccc atacggaaat cgcgtggcag gtgatctaat
    22321 ttggcgcgta aattcaggta agccagcatg atgaataacg gcggaagcat ggacgctgcg
    22381 gcggtcatat taataatggt attcatcaga tcctgcacgg tattggaacc gagcatcggg
    22441 ataatcatca gcgggatgac gatcagaaac tggatccatg ctgcgcgcgc cggaacgccg
    22501 ttttcgttaa gttcgacggt tttcttacca aagatgcctt ccgggatttc ggagaagaaa
    22561 attttcaccg gcgttgcggt ccacatcagc agggaaccga acattgcggt aaaggacacc
    22621 agaccgacaa agcgattcat caacgcttcc ggcagaccaa aatacgccgc catgccgtgg
    22681 aatacctgca ccgatccgcc ggtaaatttc aactctttgc tgctgacgaa gacgttaatc
    22741 agcaccgagg agacggaata cagtacgccg ataaaaatcc cggcgaggat gatcacttta
    22801 acgaacgatt tcgaaccgcc tttgacgtcg ttaacgtaca ccgcgacgga ctccgcgcca
    22861 cctgcggcca taaagatcca ggtggtaacg ccgaggaacg cccagttgaa gttcgggatc
    22921 atcgcgtcaa cggtgatcgg atcggcaggc tgtacgccgc caaccagtgc cgtacctgcc
    22981 agtaaaatgt aggagagcgt taacagcagc atcagcgttg aagtgacgga ggtaattggc
    23041 cccagcattt tcgccccgtt ggtggaaacc caggtggaga aggcgaacag caccatactg
    23101 ataacggtgg tggcaaccgg cgtcataata tattcgtagc cgaggaaggc atacgaagca
    23161 taagcaataa cgcgcggcaa cagcgaggtg aaaaagaaca gatttacgaa ccagtaagtg
    23221 taggcagtaa taaatgccca acgtccgccc agcgaacttt ttacccacgc gtagacaccg
    23281 gcttctgagt ttttatttaa cgaaacaaat tctgcgatga tcagacagaa gggaataaaa
    23341 taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca gtccaagctc aatattatta
    23401 ttgataacgt tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa gccgaatttg
    23461 ccgattgtat tacgtttggt atcagacata acacatctcc gaaaatgtgt agggtagcgg
    23521 tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt atggaaataa ccatcttcga
    23581 tggtgacttt gagaaccact tttttgaccg cgttattgca gataaaccga tacgcttcat
    23641 ggatatcgca aataacgatt tgcccttcgt ggacctctac ggtttctccg cagcctttta
    23701 aatattcacg gtcagtttca tcacgataat attccactac ctgtaatgtc tctttcggcg
    23761 catattcaat tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag cgtcgatgcc
    23821 cggtaaatag cgcatcggtc gcggagtcat tttccacacg gtaggtcaat gaatcaccga
    23881 tggagtgggc gacgccaggc tgaatgttgt cgatattttc aatcgcttca acgcagcgtt
    23941 gccacttctt gccagaggcg taaatctggc ggaactgttc taagttatcg atgatcctca
    24001 ttgcttattc tcgctgtgca aattcgtgga aaagaaccct gcgccgaact catacgacgc
    24061 caggctttgc gcggtagctt ctccgccaga aaccggcagc aacgtaaagc cgtagctgaa
    24121 gtcacggaac cagacgcgcc aggagtccag cacctcgctg ccccaggagt tggagccgag
    24181 gccaagtagc tggtggtcga ggttcagggt gatgtcatca ctgcgctgca gctcgttaca
    24241 gtgctgggca gcgtggatgt tttcctgggt atagtgccag gcgctgaagt taattgggcg
    24301 ctgcggaacc accagcagac cgttgccgtg gcggttagtc agtgccgtcc agcggacatg
    24361 ctgacggttg ccgttgttct gcgggaaggg atagttctcg aacatggcat cgacggtgct
    24421 gcgccagata tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc ccggtccacg
    24481 accgtaatac gccacctgat cgtattcgcc gttaatcccc atggtgaaac cgatgcacgg
    24541 aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa cctggccatc
    24601 ggcagcgatg cgccagatgt aggtacagcg catcccgaag tcaaacaccg gcggtgcaat
    24661 aactgtgcgg ctgatgatca acacttcacc atcgctctgt tctacggcaa agtcgcgcag
    24721 atgttcctgc atgatctgca aatgattcgg ctgccacaac ccttcgtact cctgcttgtg
    24781 gttgtcgatc atcggcttga agaagttgat ctttggctcg cgggtcagca gcgactcgcc
    24841 atttacctgg caggatgtcg gtttgccact catttttgag aaggtgatcg cgaagttgta
    24901 gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg cattatttgg
    24961 tgcgaaaggc actggctgcg cggtgttttc cttcagcggg aactgataag tggcgataga
    25021 atgcccggct tcgctgtagc gggtgcggga atctttggtc accgtaatgt tgaggaacgc
    25081 ttcgcgggcg tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt tcggcgcaac
    25141 gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca cctctgcgtg
    25201 cagggtgtag tcatcaagcg tggtaaacca cagtttattt tcgactttca actcgccgcg
    25261 agtcagcgcg tggattttta ccggcgcgat aacctgtttg tactctttca ggcccgatcc
    25321 cggcgtctga tcggaataga tcaaaccatc aagacagaag ttatagttgt tgggatagtc
    25381 gccgtagtcg ccgccgaatt tataccagac attgccgtgg tcgtcctgtg cctggatccc
    25441 gtggtcgcac cactcccaga cataatgacc ctgaatgcaa tcgtgcttat agaagacgtt
    25501 ctggtactcc gtcagcccgc ccggtccgtt ccccatcgca tgagcatatt cacagatgat
    25561 gcgcggcttc ggatgcgggt attcaccaaa ctcattcatc agcggcacgc gggtgtacat
    25621 ggtggaaata atatcgacca cttcagcatc gcgatcttct tcgtaatgaa ccagtcgcgt
    25681 gtcatccagc gctttcgccg catggtacat cgcgcggatg ttacagccat agccggattc
    25741 attgcccagc gaccagatga tgatcgacgg atggtttttc tgtgcgtgga tatggcgaac
    25801 aatgcgctcg acgtagactt tttcccactg cggatcgtcg gtaatacggc tgatatcgcc
    25861 gacattagca aagccgtgcg attcgacgtc ggtttccgcc atcacaaaca ggccgtagat
    25921 atcacacagt tcgtaaaaac gcggatcgtt cgggtagtga gcggtacgca cggagttgat
    25981 attgtgctgc ttcatcaact ggagatcttt ctcgacgcga tccattccaa cggcgcggcc
    26041 tttgcgatga tcgttgtcgt gacggttgac gccgtgcagc atcacataac ggttattgat
    26101 ccagaacaga ccgtcgcgca ctttgatatc acggaagcca acgcgttgtg gcaccacttc
    26161 cagaacgttg ccgttggcgt ctttcagcgt catgaccaga tggtaaagat aaggggattc
    26221 tgctgaccat tgctgcggct gttcgacagt aaaagcaaag ctggcgctgg tcagtttttc
    26281 aattgccaaa tgatcaatgg cgctgctgtg caccacgcgt tcgccatcaa acagggtata
    26341 ttccagcgtc gtgacgacag gggaggcggc gagattttcc agcaccactt cgcaggaaag
    26401 cgtggcatcg caataggctt cgtcaaagtc ggtacgcaca gtgaaatcgt taatatgcgt
    26461 taggtgtttt ccgaccagat aaacatcgcg gaagatcccc gccgaccacc acatatcctg
    26521 gtcttccacg taggtagagt ccgcccactg catcatgcgc acacacaaca ggttgtcgcc
    26581 ggttttaacc atcgcgctga tgtcaaactc tgcggtcagg cgactgccct tgctgaaacc
    26641 cacatactga ccgttaacgt agacttcaaa ataggtttcg acgccgtcaa atttaatcag
    26701 cgtctgttta ccctgccagc cgtcgctgag ggtgaaaata cgttgatagg caccggttgg
    26761 gttatcgctg gggacaaacg gcacatcgat ggggaacgga aaaccttcgt cggtatattg
    26821 cagtttgccg tgaccttcca tttgccacat ggcggggacg gtaatatgcc cccagtcagc
    26881 cattaactca gaggtgaagg cttctggtac ttgcagcgga tggtcaaaaa agtggaaatt
    26941 ccactgactg cttaagggca gaaacaggct gctggtttcg cgggcaaagg tacgcgcttg
    27001 cgcaacagaa tcatatgaaa aaaagtacgc acgcggcgca agtcggtttt cgtgggtgag
    27061 ctgaatgttt tcccagcgat tcatagggcc tcccggtaga gagaaatcgg aaccattcat
    27121 ggtgatggta gtttgtgtaa ttgctggggg gttagtaaaa tttttactaa aaagcagcag
    27181 gtaaatgcat ttactttaac tggatcacga aaataaacgg cagcactgtc cggcaaaaat
    27241 gattgccgga cagtgtgtaa ggggatttaa cgggtcgtgc cgcgcagttt taatttgctg
    27301 ggaacgaaga ctagcagcgg cagcgcgcga ccatcgcggg ctttttcata caccaggtta
    27361 acgccctgac ttcccatcat ctcggaatgg atgcgcacgg tggagagcgg cggaaaggta
    27421 aatcgcgcgg tggggatatc gttaacgctg ataagcgaaa tatcttgtgg gatgttcaga
    27481 ccacgttcat gaattgcccg cagtacgccg atagcaatgg aatcggaagc aacaaacagt
    27541 gccttcggat agtcttcccg cgccagcatt tgttttgcca gttcataacc cgacgaactg
    27601 gaaaaaccgc cgcgccagat atcctcttcg cgtaccactt gtttcagtcg gccatattcc
    27661 gcaaaggcga cctcacgaat atccgccttg ccaggctcat cttcaccgcc aataaaacca
    27721 atacgattaa cgccctggtt gatatagaag tcgatgattt ctttactgat gcgtgccaga
    27781 tcgatatcca ccgcatcgta accgctgccg ggttcgtgaa agtcgataaa acagatattg
    27841 tcggtcaacg cgctggcagc ggcgcgcagg gcgggcgtgg gtttgccgac aattaaaata
    27901 ccggtgacgt ttttaatgtc tggtaatccg ttgtgttcat aacagttggt cagttcgatc
    27961 gccagctttt cgcactgagt ttcaatgccg tggcggatcg ccagatagta aggatcgttg
    28021 atctccagct cctgctggta gctgtagata gccagaatat ggtgttggtt gactgcgcct
    28081 gtctgtagtt tacgggcgct actggtcttg tactccagct tttcggcgat ctcgagaatg
    28141 cgatgtttcg tctcttcttt cacattcaat gtcggatcgt catttaagac cctggatact
    28201 gtcgccaggg atacgccagc ttcgattgcg atgtctttta atgttgccat gtttaccttt
    28261 tttactttcc ctgatgtgcg cccccattgt agaggaagtg cttatcgggt cacctgtttt
    28321 cagtaaataa agcgcgtttc tttgacgctg taacggtaac acagcggctt aggaatgtga
    28381 tcacgcctgc attctccatt tttagcctac aggattgaga aaaacttttc tatagtttag
    28441 taaaatttta cccgaaggga ggaagcaatg gaaaccgtgg cttacgctga ttttgcacgt
    28501 ctggaaatgc gcgtcggaaa gattgtggaa gtgaaacgcc atgaaaacgc cgacaagctg
    28561 tacatcgtac aggttgatgt ggggaaaaaa cactgcaaac cgtgaccagc ctggtgccgt
    28621 actacagcga agaggagctg atggggaaaa cggtggtggt cttatgcaat ctgcaaaaag
    28681 cgaaaatgcg tggtgaaacg tcggaatgca tgctgttgtg tgcggaaacc gatgacggca
    28741 gtgaaagcgt gttgttaacg ccggagcgga tgatgcctgc gggcgtacgc gtcgtataaa
    28801 aagatcggat ggcgacgtcg tatcgccatc cgatttgata ttacgcttct tcgacactta
    28861 ctcgcatggc cgccagcgcc ttacgcgccg ctttgatcac cagttcacac tgttcaatgg
    28921 tcagtgtcag tggcggttca atgcggatcg ttttggcgtt attgagcgtt ccggccacca
    28981 gtacgcgctg gcggaacatc tcgctggcaa agttatagcc gatttcgtta tcaacaaact
    29041 caatcgccat caacatccct ttaccacgcg cttcctgtac cagatcggga tattcccgcg
    29101 ccagttgacg gaaaccgtcc agcaacatat cgcctttttg ctcagcctga gccggtaagt
    29161 tctgctccag caacacattg atggtcgcca gcgccgccgc acaggccagc gggttgccgc
    29221 caaaggtggt ggtatgcagg aatgggttgt cgaacagaac tgaaaacacc tcttcagtgg
    29281 cgatggtcgc gccaatcggc atcacgccgc cgccgagggc ttttgccaga cacagaatgt
    29341 ccggctgaac gttctcatgt tcgcaggcaa acatcttgcc cgtgcgcccc atgcccgttt
    29401 gtacttcatc gaggatcatc agtgcgccga actcatcgca cagcttacgc acggcggtga
    29461 gatagcccgg cggcggcaga attacgccac cttcgccctg aatcggttcg aggatcaccg
    29521 cagccacatc atcaccggtt tttttgcact cattaagagc cgtgcgcatg gcttcgatat
    29581 tgccaaacgg cacatgacgg aaccctggca gcagtggcat aaacggtttg cggaaggtcg
    29641 atttcgccgt ggccgacagc gcgccaagtg atttaccgtg gaacgcgccg ctggtggcaa
    29701 taaaagtaaa tttgccgcgc ggcgactgat aagccttcgc cagcttcagc gccgcttcaa
    29761 cggactcggt gccgctatta cagaagaagc tgtatttcag tttaccgggc gttagcgcag
    29821 caagggtttt cgccagcata gcccgtaacg gatcgagtag ctcctggctg tgcagtggtt
    29881 gtttcgcaag ttgattctgt acggcggaaa ccacaactgg attacggtgc cccacgttga
    29941 aaattccaaa acctcccagg cagtcgataa actcctgtcc ctgggtgtcg acaagcgtat
    30001 ttaaacctcc cgcttgccac tctacggctc cgtaatcccc gccggcggta acagatttgc
    30061 gatactctaa aaaccccgga ttgacatgct ctttgaagta ttcaatcacc tctcggttaa
    30121 gtgctttcat ctcctcatga tccagcgttc gcttctcaat gagattcagg gcgtgggcgc
    30181 tgcacgctaa agccgatgcg ctcgaaggta acctgttcaa aatatgctcc ggaggctcgc
    30241 gtatcacatg ataccgattt aagtattgca gggattgcgc cactccggct gcgatcgcga
    30301 aaatcgggat aaacacaaat gcggttatca tttgtgcaat atttaatgct atccgttaac
    30361 atttgttgtt attttgcacg gtttttgtcg ggtatggatt tgatgattgc tgcgcaaatg
    30421 cacgttttca gggcaataat tgcctgattt ttgggcaaga taattaacgt tttcgtatag
    30481 taacagagtg aaagtatttt aaatcaaaga attagatttg tgctttaatc gcaaattgcg
    30541 atctaaatca aattaatcgg ttaaagataa ccgcagcggg gccgacataa actctgacaa
    30601 gaagttaaca accatataac ctgcacagga cgcgaacatg tcttctcatc cgtatgtcac
    30661 ccagcaaaat accccgctgg cggacgatac cactctgatg tccactaccg atctgcaaag
    30721 ctatatcact catgctaatg acacttttgt gcaggtgagc ggctttacct tgcaagagtt
    30781 acaagggcag ccgcacaaca tggtgcgtca cccggatatg ccaaaagcgg cgtttgcgga
    30841 tatgtggttc accctgaaaa aaggggagcc ctggagcggc atcgtgaaaa atcgccgcaa
    30901 aaatggcgac cattattggg tgcgggccaa tgcggtaccg atggtgcgcg agggaaaaat
    30961 cagtggctat atgtcgattc gtacccgggc gacggatgaa gagattgcgg cggtggagcc
    31021 gctgtacaaa gcgttgaact ccggacgtac cagtaagcgt attcataaag gcctggtggt
    31081 gcgtaaaggc tggctgggta aactgccttc attaccgctt cgctggcgga cgcgtggagt
    31141 gatgaccctg atgtttatct tgctggcggc catgctttgg tttgttgctg ccccggtggt
    31201 gacgtatttc ctctgtgtgt tagtggtatt gttggcaagc gcttgttttg aatggcagat
    31261 tgtgcgcccg atagaaaatg tcgcccgtca ggcactgaag gtggcgaccg gagagcgtaa
    31321 tagtgttgag cacctgaatc gcagcgatga gctggggctg acattacgcg cggtagggca
    31381 gcttggcctg atgtgccgtt ggctaattaa cgatgtctca agccaggtgt ccagtgtcag
    31441 aaatggcagt gagacgctgg cgaaaggcac cgatgaactg aacgaacata cccagcagac
    31501 agttgataac gttcagcaaa cggtggcgac catgaaccaa atggcggcgt cggtgaaaca
    31561 gaactctgcc acggcgtcgg ctgccgataa actttctatc accgccagta atgcggcagt
    31621 gcagggtggg gaggcgatga ccacggtgat caagacaatg gacgatatcg ccgacagtac
    31681 ctagcgcatt ggcaccatta cttcgctgat taacgatatt gcgtttcaga ccaatattct
    31741 ggccctgaat gcggcggtgg aagcggcgcg tgccggcgaa cagggcaaag gttttgcagt
    31801 ggtggcaggg gaagtgcgtc atttagccag ccgcagcgct aatgctgcca acgatattcg
    31861 caagctgatt gatgccagtg ctgataaggt gcaatccggt tcgcagcagg tacacgccgc
    31921 cggacggacg atggaagata ttgtggcaca ggtgaaaaac gtcacccagt tgatcgccca
    31981 gattagccat tcaacgctgg aacaggccga tgggctttcc agcctgaccc gtgcagtgga
    32041 tgagcttaac ctgatcaccc agaaaaatgc cgagctggtg gaagagagtg cgcaggtgtc
    32101 ggcgatggtg aaacaccgcg ccagccgact ggaagacgcg gtgacggtgc tgcattaatc
    32161 ggtatgccgg atccggcggt tggagcacaa tgcctgatgc gatgctggcg catcttatca
    32221 ggcctgcggg gtgtgtagcg ggtgtaggcc ggataaggcg cgtcaagcgt cgcatccggc
    32281 aattgcaccg cgccactggc ggatgcggcg attaatccag ttgcgtaata tcaaaagcgg
    32341 cgcggtcgat caccgcaatg atctttttta tttgtgcatc actgatatcg ctctggttga
    32401 cgcgtaaatc cagcaccgct ttaaaattat ccagcgcccg cttcatttgc gggttctggc
    32461 gcagcgccgc gccaacgcaa cgcgctttga tgcgttcttc aatcatctcc acttgttcgc
    32521 ggttttcttc cagccactgc gcgccttgtt cggtcagcgc aatctgcttc ttaccacctt
    32581 cctcttcgcg gatggtaatc agcgactgct cctgcagaaa atccagcgtc gggtagatga
    32641 cgcccgggct tggggtgtaa ttcccctggg ttagattctc aatcgcttta atcaattcgt
    32701 aaccgtggct gtcatcgcgc gagagaatat ccagaatcac cagacgcaat tcaccgtgac
    32761 caaagaaacg ttgccgacga ccgccgccgc gaccgtggcg accaccgcaa cattgaccgt
    32821 gttcatgctg gtgaccgtgt ccgcagtgct cgtgttctga cttctcacct ttgcagcagc
    32881 cctcatggcg tggctggcct tcatgtttac aacacccttc gtgatgatgg ctcatatcag
    32941 cctccttttg cttgattaga tatatctaat ttatatctga tgtgagcatt actgcaagta
    33001 caaaaatgat ttttaaacgt ggaagcacct cacatctagg ccggataaga cgttcacgcc
    33061 gcatccggca gcattgctat ggcactgaat gccttcacat acttcagaca tatcagttct
    33121 acaaatcgct tgcatttatc atgattaaca atcattatca tttgcgaatt ttatttagat
    33181 atatctgatt accatcacga aggcgataac aatgaataac accccccgct acccgcagcg
    33241 cgttcgcaat gatctgcgct tccgtgaact gactgtgcta cgcgttgagc gcatcagcgc
    33301 cggttttcag cgcattgtcc ttggcggcga ggcgttggac ggttttacat cgcgcggctt
    33361 tgacgatcac agcaaactct tctttcctca acctgacgct cactttgtgc cgccaacggt
    33421 aacggaagag agcatcgtct ggccggaagg accacgccca ccgtcgcgtg actatacgcc
    33481 actgtatgac gaactacgcc atgaactggc gatcgatttc tttattcacg acggtggggt
    33541 cgccagcggc tgggcgataa acttacggtg gcaggtccgc gcggttcgct ggtggtgccg
    33601 gaagattacg cgtatcagct gtatgtctgc gatgaatccg gaatgcccgc actgcgccgc
    33661 cgcctggaaa cgttgagcaa acttgccgtt aaaccgcaag ttagtgcgct ggttagcgtg
    33721 cgggataacg cctgtcagga ttatctcgcg caccttgacg gttttaatat cgaatggctg
    33781 gcacatgatg agcaggcggt agatgcgcgt ctggcgcaga tgcaaatccc tgccgatgat
    33841 tacttcatct ggataaccgg cgaaggtaaa gtcgttaaga atttaagccg ccgctttgaa
    33901 gcggaacagt atgacccaca gcgggtacgt gcagcggctt actggcacgc aaagtaactg
    33961 atatataaaa agaaaaaggc tgacgatttc tcgtcagcct ttgctatatc tggtggcccc
    34021 tgctggactt gaaccagcga ccaagcgatt atgagtcgcc tgctctaacc actgagctaa
    34081 ggggccatgg tagcggatta taaagtaact ccgtgtcgca atccagccat taccgcgcgc
    34141 ctgctgtttt tataaacaat gtattttcaa tccgttatac ttttcctggt gatgttagaa
    34201 aggagtaaat atggttgagg atattttggc tccagggtta cgggtcgtgt tttgcggtat
    34261 caaccctggg ctttcatccg ccgggactgg ttttcccttt gctcatccgg caaatcgctt
    34321 ctggaaggtg atatatcagg ccgggtttac cgaccgtcag ttgaagccgc aggaggcaca
    34381 gcatctgctg gattatcgtt gtggcatcac caaactggta gaccgtccaa cggtgcaagc
    34441 caatgaagtt tcaaagcagg agctgcacgc aggtgggcgt aagctgattg aaaaaattga
    34501 agattatcag ccgcaggcgt tggcgattct gggcaaacaa gcatatgaac agggattcag
    34561 ctagcgcggt gcacagtggg ggaaacaaac gctcaccatt ggttcgacgc agatttgggt
    34621 gctgccaaat cccagcggtt taagtcgcgt ttcactggag aaactggttg aagcgtatcg
    34681 cgagctggac caggcgctgg tagtgcgtgg gcgataa
//
LOCUS       DAOFMX010000041        34281 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.041, whole genome shotgun sequence.
ACCESSION   DAOFMX010000041 DAOFMX010000000
VERSION     DAOFMX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.041"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..578
                     /locus_tag="QBA20_002194"
     CDS             <1..578
                     /locus_tag="QBA20_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6292720.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(575..1711)
                     /locus_tag="QBA20_002195"
     CDS             complement(575..1711)
                     /locus_tag="QBA20_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDQ6292721.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1717..2040)
                     /locus_tag="QBA20_002196"
     CDS             complement(1717..2040)
                     /locus_tag="QBA20_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDQ6292722.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2223..2366
                     /locus_tag="QBA20_002197"
     CDS             2223..2366
                     /locus_tag="QBA20_002197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292723.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="QBA20_002198"
     CDS             complement(2585..4123)
                     /gene="aldB"
                     /locus_tag="QBA20_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDQ6292724.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4288..5337)
                     /gene="yiaY"
                     /locus_tag="QBA20_002199"
     CDS             complement(4288..5337)
                     /gene="yiaY"
                     /locus_tag="QBA20_002199"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDQ6292725.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVENGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVKEEDFAVLATNALKDACGFTNPI
                     QATHEEIVVIYRAAM"
     gene            complement(5527..7371)
                     /gene="selB"
                     /locus_tag="QBA20_002200"
     CDS             complement(5527..7371)
                     /gene="selB"
                     /locus_tag="QBA20_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDQ6292726.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7368..8759)
                     /gene="selA"
                     /locus_tag="QBA20_002201"
     CDS             complement(7368..8759)
                     /gene="selA"
                     /locus_tag="QBA20_002201"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDQ6292727.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEAIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8857..9465)
                     /locus_tag="QBA20_002202"
     CDS             complement(8857..9465)
                     /locus_tag="QBA20_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDQ6292728.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9536..10621)
                     /locus_tag="QBA20_002203"
     CDS             complement(9536..10621)
                     /locus_tag="QBA20_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDQ6292729.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10624..10986)
                     /locus_tag="QBA20_002204"
     CDS             complement(10624..10986)
                     /locus_tag="QBA20_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDQ6292730.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11523..13436
                     /gene="mtlA"
                     /locus_tag="QBA20_002205"
     CDS             11523..13436
                     /gene="mtlA"
                     /locus_tag="QBA20_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDQ6292731.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13531..14679
                     /gene="mtlD"
                     /locus_tag="QBA20_002206"
     CDS             13531..14679
                     /gene="mtlD"
                     /locus_tag="QBA20_002206"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDQ6292732.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14679..15266
                     /gene="mtlR"
                     /locus_tag="QBA20_002207"
     CDS             14679..15266
                     /gene="mtlR"
                     /locus_tag="QBA20_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDQ6292733.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15278..15487)
                     /locus_tag="QBA20_002208"
     CDS             complement(15278..15487)
                     /locus_tag="QBA20_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292734.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15771..16133
                     /locus_tag="QBA20_002209"
     CDS             15771..16133
                     /locus_tag="QBA20_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDQ6292735.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16677..17360
                     /locus_tag="QBA20_002210"
     CDS             16677..17360
                     /locus_tag="QBA20_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDQ6292736.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17404..22066
                     /locus_tag="QBA20_002211"
                     /pseudo
     CDS             17404..22066
                     /locus_tag="QBA20_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22434..24089
                     /gene="lldP"
                     /locus_tag="QBA20_002212"
     CDS             22434..24089
                     /gene="lldP"
                     /locus_tag="QBA20_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDQ6292737.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            24089..24865
                     /gene="lldR"
                     /locus_tag="QBA20_002213"
     CDS             24089..24865
                     /gene="lldR"
                     /locus_tag="QBA20_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDQ6292738.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24862..26052
                     /gene="lldD"
                     /locus_tag="QBA20_002214"
     CDS             24862..26052
                     /gene="lldD"
                     /locus_tag="QBA20_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDQ6292739.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26248..26721
                     /gene="trmL"
                     /locus_tag="QBA20_002215"
     CDS             26248..26721
                     /gene="trmL"
                     /locus_tag="QBA20_002215"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDQ6292740.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26774..27595)
                     /gene="cysE"
                     /locus_tag="QBA20_002216"
     CDS             complement(26774..27595)
                     /gene="cysE"
                     /locus_tag="QBA20_002216"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDQ6292741.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27675..28694)
                     /gene="gpsA"
                     /locus_tag="QBA20_002217"
     CDS             complement(27675..28694)
                     /gene="gpsA"
                     /locus_tag="QBA20_002217"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDQ6292742.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28694..29161)
                     /gene="secB"
                     /locus_tag="QBA20_002218"
     CDS             complement(28694..29161)
                     /gene="secB"
                     /locus_tag="QBA20_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDQ6292743.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29224..29475)
                     /gene="grxC"
                     /locus_tag="QBA20_002219"
     CDS             complement(29224..29475)
                     /gene="grxC"
                     /locus_tag="QBA20_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDQ6292744.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29617..30048)
                     /locus_tag="QBA20_002220"
     CDS             complement(29617..30048)
                     /locus_tag="QBA20_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDQ6292745.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30293..31837
                     /gene="gpmM"
                     /locus_tag="QBA20_002221"
                     /gene_synonym="pgmI"
     CDS             30293..31837
                     /gene="gpmM"
                     /locus_tag="QBA20_002221"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDQ6292746.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31847..33130
                     /gene="envC"
                     /locus_tag="QBA20_002222"
     CDS             31847..33130
                     /gene="envC"
                     /locus_tag="QBA20_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDQ6292747.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            33134..34093
                     /locus_tag="QBA20_002223"
     CDS             33134..34093
                     /locus_tag="QBA20_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDQ6292748.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(34080..34281)
                     /locus_tag="QBA20_002224"
                     /pseudo
     CDS             complement(34080..34281)
                     /locus_tag="QBA20_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         8527 a         8980 c         8744 g         8030 t
ORIGIN      
        1 ttgagcgtaa tgccttttat gaaggcgtcg aatttcctga tgattgtgat gttttaatca
       61 gctacgctcc accaaaagat gaatcgctgg ttgccagttt tattacacaa tatgccgtaa
      121 ccgcttatgc cagccagcgc tatcttgaga aacatcctat tagccgtcct gacgaactcg
      181 aacatcactc ctgtattttg atcgactcga tgatgatcga cgacgcgaat atctggcgct
      241 tcaacgtcgc cgggagtaaa gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg
      301 ataacacgca atcggcgctg gagctggcac gaaatcacct ggggattgtg tttgcgccag
      361 acaaaagtgt gcagagcgat ctgcaagacg gcacgctggt gccctgcttt cagcacccgt
      421 atgaatggtg gctggatctg gtggctatct tccgtaaacg ggaatacctg ccctggcggg
      481 tccagtatgt gctggatgaa atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc
      541 aactgcggcc tgaacaagcg gcggaaagcg aagattaatg atcgagataa aggtaatgca
      601 cccagctggt catgcgtaat agcactttgc gcatcaccga gacatggctg aaatgatcag
      661 agtagaccgc cacctgggcg taaataccgt ctggtaaagc gctcaaatca gtgtgctcat
      721 ccagttcaat ggtcgcgata acgccatctg aacccggcgc tgtgtttaac gtctgtaagg
      781 tgccggtcga ctgataagct ccgccgggaa cagcaggact aatggctgcc agcttgccgc
      841 tgaatacctt acctggcaga gcattaaaca ccacttccgc atcgtcgcca ggggccagtc
      901 gcagcaagga gttctgacgg aactgcgcca caatttgtcg tttctgatcg ggtataaaca
      961 ccatcaccgg acgtagcggc agcgacgcgg cataggtacc cggacgaatc agcacctggg
     1021 tcacataacc atcgctcggc gcacgcacta tcgtctgctc aaggttatat tttgcttccg
     1081 cgagctgcgc tttcaggctg gcgatttgag aatgttcacc caacaccagg ctatccagct
     1141 ggctctggat ctgtttttgt tccgccgccg atgactttac ggaggcttcc tgcgccagat
     1201 aattttgccg cgccacatca atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc
     1261 cgcgtgcgta acgctgatac tctttagcga atttatcccg cgtggccttt gcctgctgag
     1321 tattcgccgc catctcatct aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa
     1381 cgatatccgc catcagccga tccacccgcg cctgataacg cgtcgggtcc agtcgaaata
     1441 gcacctcacc ttttttaatc agcgtatttt tcttatccgt cacttcgatc accacgcctg
     1501 tcacctgtgg gacaacagga atagaaataa ccgctttttg cgccttgaag gtatacggat
     1561 ggttatagtt cattaacaga attaaaccac tgacaataaa tatgccaccc agggccgctg
     1621 tggaaatggt ccatttattt acaggaattt tgaagatctt aaacattgcc catgcaaaag
     1681 ccacataggt caaaataatc agtaaatcca taagtattac tcggcagaag aggatttgat
     1741 gtcggcgagc tgtttctcca gcccggcaat acgctgttgt agttgcacaa cggaggagtc
     1801 atggatttgc attccccatc cccgctccgg gcggtaaagc gtggcccaaa tccacaaaaa
     1861 tggccagata acatgcagcg taaaaagact cacccaacca gcaacatgaa tggcgtcggc
     1921 atgaggatga ttacgttttt tagcaatcag gtagggaata tcatgtaaaa taataatccc
     1981 atagaatatc accagaaata caaaaataag cactcccagt gcaaaatagt ccaggaacat
     2041 atttccctca aagaatataa aaaagaacaa ttaacgcata ttatgaaatg ccatgatgca
     2101 gtgcaatttc cttatttata taacgacata ttgtttttta tatactttct gaaatgtgct
     2161 gtctggtttt tcaacaggac gaggtttatc atattgaaat agtggaacta ttggccaaac
     2221 taatgaataa ctccagtaaa acatccacag tacagattaa gcgtattaaa ccttcaatta
     2281 tctaccgttt attgctgatt ggcctcggat caccaatggt gatttacggc ctggttcgcc
     2341 cgctcaccat cgaaacgcga gattaaactc ctgacaaaac acgccccaga aacgcacttc
     2401 tgttggcgta gtctgggtta ttgcgcacgt aggtttaaaa cgtaaattgt aacaacgtgc
     2461 gctttgttta tgccggatgc ggcgtgtacg ccttatccgg cctacggtca ggttcccgta
     2521 ggcatgataa gacgcgtaag cgtcgcatca ggcaatgaat acccaatgcg accagcttct
     2581 tatatcagaa cagccccaac ggtttatccg agtagctcac cagcaggcac ttggtttgct
     2641 ggtaatgctc cagcatcatc ttgtgggttt cgcgaccgat acctgattgt ttgtagccac
     2701 caaacgcagc atgtgccggg taagcgtgat aacagttggt ccacacgcgc ccagcctgta
     2761 tgccgcgccc catcttatag gccagattac cgttgcggct ccagacgccc gcgcccaggc
     2821 catattgcgt atcgttcgcc agctccagcg cttcttccat cgttttgaag gtggtcaccg
     2881 ccagcaccgg gccaaaaatc tcctcctgga acacccgcat attgttctga ccaaacagaa
     2941 tcgtcggttc gaggtagtag ccgtctttca gttcaccttc cagcagcttg cgccgcccgc
     3001 ctgtgagcac gtcagcgccc tcttttttac cgatatcaat gtagttgagg atggtttcca
     3061 gttgcccgtg agaaacctgc gcgcccattt gcgtcacgct gtcgagcggg ttaccgctgc
     3121 gaatgctttc gacacggcgg atggcgcgtt ccataaagcg ttcgtagatt gactcctgca
     3181 ctaaggcacg actcggacag gtgcaaactt cgccctggtt aaaggcaaac agtgcaaagc
     3241 cttccagcgc tttatcgaaa aaggcatctt cttcatccat cacatcagca aagaagatat
     3301 ttggcgactt accgcccaac tccagcgtca ccggaataat gttttgcgtt gcgtattgca
     3361 taatttgttg gcccacttcc gttgagccgg taaacgccac tttggcgatg cgtttcgagg
     3421 tcgccagata ttcgccaatt tccccacctg cgccattgac cacgttcacc acgcccggcg
     3481 gcagtaaatc accgacaatt tccattagca gcagtacaga aagcggggta agacgtgcgg
     3541 gtttcagcac cacacagttg cccgccgcca gcgcgggagc cattttccag ctcgccatca
     3601 gcagcgggaa gttccacggg ataatctgcc ccaccacgcc taacggttca tggaaatgat
     3661 aggccacggt ttcgctatca acttcactga tcccaccttc ctgcgcccga atacacgagg
     3721 cgaaatagcg gaaatggtca atcgccagcg gtacatccgc agcactggtt tcgcgaatgg
     3781 gtttgccgtt atcccaggtt tcagctgtcg ctaacagctc gaggttttgt tccattcgat
     3841 cggcaatctt aaacagaatc gccgcgcgat cctgcaccga ggtgtgcgcc catttatctt
     3901 tcactttgtg cgcagcatcc agcgccagat cgatgtctcg tttgcccgaa gacgccactt
     3961 cgcacagcag ctgcccggtc accggcgtca gattctggta atactcacca tcggcagggg
     4021 ctacccattc accgccaata aagttgtcgt agcgggcttt taacttgatg gggaaaccat
     4081 actcgccggg cttaatctgt gctgaagggg gattattggt catgatcgtc tccttgcggt
     4141 gtgaggtata acaagggtag acgtgactgg cgaaatcttc gccagtcggt aacagcttta
     4201 cgacagctat cacgaattta cgggcaagga ttacagagat agttgaaaaa gcggctgaca
     4261 atttgccagc cgttgttgga atgatgatta cattgcggcg cgataaatca ccacaatttc
     4321 ttcgtgagtt gcctggatcg ggttagtaaa gccacaggca tctttcaggg cattagtcgc
     4381 cagaaccgcg aaatcttctt ctttcacgtt cacgcccatt gcagcggcac agtcacgcag
     4441 acgtgcagcg gcgactttgc tgttgaatac ctgaacatgc ggcagcagaa cggcgttaca
     4501 tacaccgtgt ggcaggttgt agaaaccgcc cagctggtgc gccatcgcat gaacataacc
     4561 cagagaagca ttattgaacg ccataccggc aaggaactgg gcataagcca ttgcttcacg
     4621 cgctttcgca ttactgccat tttcaacggc taacggcagg ttttcggcaa tcatggtcac
     4681 ggctttcagt gcacaagcgt cagtgatcgg cgtggcggca atagaaacat acgcttcgat
     4741 agcgtgcgtt aaggcatcca tacccgttgc ggcggtcagt gacttcggca taccaatcat
     4801 cagagaggag tcattgacag aaagcagcgg agtcacgtgc ttatcaacaa tcgccatttt
     4861 gttatgacgc gcttcgtcag tgatgatgca gaaacgggtc atttcagagg ccgtacccgc
     4921 cgtggtattg atggcgatca tcggcagctg cggttttgca gagcggtcaa cgccttcgta
     4981 atcgcgaata tcgccgccat tggctgccac cagcgcaata ccttttgcgc agtcgtgtgg
     5041 agaaccaccg cctaaggaga tcacgctatc gcaattattt tctttaagta atttcaaacc
     5101 tgcggcgacg ttttccgtgg tggggttagg ttgggtgcca tcataaataa cgctaaaaat
     5161 attgcgttct tccagtgctt tttgcacatc acccgccata cctaatttcg ttaacatatt
     5221 gtcagtgaca attaaggtac gggtaaatcc ataatctgcc atcatattca ttgcatcagt
     5281 caatgaatca gcgccgatga cattcacaga aggaataaag aaagttgaag atgccataac
     5341 acactcctga aagtgtaaaa aagggtgggc agcttactga ggattttcat tcgaaaacat
     5401 gatcattatt attaattatc aaaattaatc taataaaaag cacattgtat tcattaaaca
     5461 atgtgcttta tttaaaatca taagaaattt gtattaattt attttaaaaa ttaattaatc
     5521 atttccttat ttttccggaa ataataatgc gtcgcgcaat aaatgatcat ttccacgacg
     5581 acgcgtaaag ccaatgcggt caaaatattc cagaatctga attgccagct ttcggcctac
     5641 gcctaagcga tcgcggaaat ccgccgcgca ggttgaacca cactcctgat cgagatcgcg
     5701 gatcatattg gcaaattcga caatccgatc gttacggtaa taacgatctt tgacgatcgc
     5761 ggtaattatt ccttgctgcg ccgcctggcg tagcgtcagg cgcattgcct gctcgtcggt
     5821 acccgtctct tttgccagat cacgcaccca ccacggctcg tcaccaaaca gcggctctgc
     5881 tttttgccag atggcctgct gctcttcgct gaagcccgct ttgtgatccg gcagatgcag
     5941 ccaaccgtga tgactgagga tgtcgccgct ttcgcgcatc ttttcaatca gcaacagtac
     6001 tagcgcttca tcttccattg gcaacgccat acgtcgcaga cgttcgcgcc caggcccagg
     6061 ttcatcgcga tgttgctcat gataagtcgc taatgtgtcg agaattttcc gttgccagcg
     6121 agcggcaacc ggcgcattca acaagctata accagcctga atatagccag gctgttgcag
     6181 cagttcgcgc atcccttcgc cgttgagctg gcgcgcccag gcgaaatcag gaagattaac
     6241 cgcgccgcgt tgcaggtgaa cggctaacgc ttcgctatca ttctgtgccc tggcgaggga
     6301 agccagccat tgcagatact ctggtttacg tttaccgcga cgcggcgggt taagcatcac
     6361 gacgcgcgct ccggccagcg tgttgcgggc agagatatcg cgcaatacca ggcggtcgtt
     6421 atctgccagc cataacgggg tgtcgaagac cagttcagca aggttatctt ccagcagtga
     6481 aacgcgtccc gtgacgtggc tggcggcgtg gtgaatatgc agcggctgcc actgggtcag
     6541 cggtgcatgg gtttgaagct cgacaatcac ccgtgtgaac ggctctggcg gcacatcggc
     6601 aagcagccag tcgccacggt taatctgctc tttttccgca tcacccgcga tgtcaagcgc
     6661 gatacgctgc ccggcattgg cggtttctgt tggctggttt tgcgcatgca gcgcacgtac
     6721 acgcatcggt ttatttacac cagtcagcca gagtgaatcg cctaccttca cttccccgct
     6781 taacgccgta ccggtgacga ccagcccggc accttttacg gtaaatgcgc ggtcaatcgc
     6841 gaggcggaaa ctatgttggc tggcgtgctc gcgttccggc aactgaagca gatgctcgcg
     6901 cagggcatca attccccgac cttcggttgc tgcggtgata aacagttttg cctcagcaaa
     6961 accgtattcc cgcagaacct cctttacctg gcgttcaacc tcatcaacac gcgcttcgtc
     7021 cacgcgatcg gctttggtca gcgccactgt cagcatcggg ttaccggtca gctgcaaaat
     7081 cgccagatgc tcacgggtct gtgccatcac gccgtcatcg cacgccacca ccaacagcgc
     7141 gtgatcgata ccaccaacgc ccgccagcat gttggaaaga aacttttcat gaccgggaac
     7201 gtcgataaaa ccaggcacgc gaccatccgg ctgcggccag taggcatagc cgagatcgat
     7261 ggtcatgccg cgcttttttt cttccggcag acggtcagca tttacgccag taatcgcctg
     7321 caataaggtt gttttgccgt ggtcaacgtg tccggcagtc gcaataatca tttcaacaac
     7381 atctccaaaa accgttgctc atcttcaagg cagcgtaaat ccagccacaa tcgtccgtca
     7441 taaatacgac caatcaccgg cactggcaat tcacgccagc gggcggctaa tgactcaagg
     7501 tggctaccgc gtccatcatg gggtgtaaac gttaatgccg cgctcggcag gcgatcaacc
     7561 ggcagcgaac cactgccaat ctgcgaaaga catggcataa cctgtaccgc aaactccgcg
     7621 ccgtaatgtg cgacaagggg agcctgtaaa cgttgtgcct ggatttgaat ggcctctgcg
     7681 ctgcgggtaa gcaggcgcag ggtcggtaat ttttcactca gagcttcagg gtgtaaataa
     7741 agacgcaacg tggcttccag cgccgcgagg gtcattttat ccgcgcgtaa tgcacgcttc
     7801 agcgggtggc tttgcaggcg ggcgatcatc tcttttttac caacgataat cccggcctgc
     7861 ggcccgccta acaacttgtc gccggagaag ctcaccagac tgacgcccgc cgcaatcaac
     7921 tcctgcggca ttggctcttt cggcaaaccg tactggctaa gatcgaccag cgagccactg
     7981 cctaaatcag tcaccactgg aacatccagc tctttgccga gcgccaccag ttcagcttcg
     8041 tctatcgctt tggtgaaccc ctgaatgctg tagttactgg tatgtacttt catcaacagt
     8101 gcggtatttt cattcaccgc ctgacgataa tcattcgcgt gcgtgcggtt ggtggtccct
     8161 acttcgtgta gggtgcagcc tgcctgacgc ataacatcgg gaatacgaaa cgcgccgcca
     8221 atctccacca gttcgccgcg agataccacc acctctttcc cgctggcagt ggccgccaac
     8281 atcaataaca ccgccgccgc attgttattg acgatacagg catcttccgc ccccgtaata
     8341 cggcacagca gctgcgccag cgcccgatcg cgatgtccgc gtccggcgtc gtccagatcg
     8401 tactcgaggg tcactggcga acgcatagcc tgcgcaacgg cttccaccgc ggcttccgcc
     8461 tgtaaagctc gcccaaggtt ggtatgcagc acggttcccg tcaggttgat caccggacgc
     8521 agcgcgctct gcgcctcttt cgtcaaccgg gcatcgactt cttgcgccca gttttcacac
     8581 cacgcaggca gcgtctggct gccacgaatc acttctcgcg cttcgtcgag catctgacgc
     8641 aacaattcca ccacgcgggt gtgaccataa gtatcacgca aagaaaggaa ggagctatcg
     8701 cgcaataagc gatcaatagc cggaagttga ctatagaggg aacgcgtttc ggttgtcata
     8761 ggaaacctgg ctgatcaagg ccctctcaca cggagaaggg cgtttaacat aaccacggat
     8821 tgtaacgtga gatgggtcag gaggacatat cgcgcatcaa gcctttggcg gttcggtgcg
     8881 agcaaaactt tcgcggctaa acaggttttc gaccagtttg actaagtgtg ggcggtcgac
     8941 acaccaacct ggcgcaacgc ggcggaaatt gagatatccg acagcacagg caatggcgat
     9001 agtcgccaga ttgaccgtat cggttttgag tgtgccatcg accagatatc cttccagcac
     9061 atccagactg cggttgattt tctcccgctg gcgtaacaat tcatcttcag actgctgcgc
     9121 cgccggacgc gcctgttcac gcaccgatac cagcccggca tccataatgc catccgccag
     9181 cgcctcaatt ttgcgcaccc gcaacgactc cagcggatcg cgcggcaaca tcgccggagc
     9241 gacattcatt aactcaatat attcagcgat gatcggcgaa tcaaaccagc attcgccctc
     9301 ttcggtcacc agcaccggca ctttccctaa cgggttaaat tgcgccacgc cgttatccgc
     9361 gttatagggc agttcattaa taaattcgaa agttatgccc ttttctaaca acagaataga
     9421 aagtttgcgt acaaacgggc tggtgtagct accgacgagt ttcatgccga gtcctttgtg
     9481 cgaggaaaaa tatcagtatg gctcaggctc acgcaaaaca caggtgatat aagtctcaat
     9541 gatccaaata aagataatgc atccaactgg tcattcttag cagcactttc cgcatcaccg
     9601 aaacatggct gaaatggtcg gagtaaaccg ccacctgggc gtagatgccg tcgggtaagg
     9661 catcgatatc atcgttaggg tccagttcaa tggttcccag cacaccgtcc gtgccgggaa
     9721 cgaccgttaa tgattgcaat accccctgcg cctgataaga accgcctggc acgacaggta
     9781 aaatactagt caatttgccg tggaacacct gcccaggtag cgcgttaaac accacttccg
     9841 catcatcgcc aggtttcaga cgtaacagcg agttttgccg aaattgggcg acaatttgcc
     9901 gtttttgctc ggggatgaag accatcaccg gacgcagcgg caaggcagct gcgtatgtac
     9961 ctgggcggat cagtacctga gtgacgtagc cattgcttgg tgcgcgaatg acagtctgct
    10021 caagattata ttttgcttca gtaagttgcg ctcttaagct cacaatctgc gattgctcgc
    10081 cgttgaccat actgtcgagc tgactctgga tctgcgcctg ctccgccacc gagcctttca
    10141 ctaacgcatc ctgcgcgagg aaattttgcc gcgcatcgtc gatgtcacgt tccgagaacg
    10201 gattcaccgc cgcctggctg cctttcaggt aacgttgata atttttaaac agacggtcgc
    10261 gctccgctga aacctgggtg gtgttggcct gcgcttcagt gagctgcgca cgcagcgtct
    10321 ttatattatg cgtcgccgtc atcaggtcag cctgaagtct gtcaactcgc gcctggtaac
    10381 gaaccgggtc gagcttaaaa agcacctcgc ccttttgaat aagctgatta ttcttgtcag
    10441 tgacttcagt aacaattccc gtcacctgtg gcgtgatagg gatcgctatc actgcctttt
    10501 gcgcggtaaa agtgtaaggg tggttgtagt tcatcaacaa aatcaaacca ctcaccagaa
    10561 acacgcctcc cagcgccgcc gtcgccagcg ccacgtaagt taaaacaatc aatagatcca
    10621 taattagatc tccgggaacg tggaatagtc agtctttttc tcggcggcga gttgatgctc
    10681 cagccgggaa attcggtcag aaagtgcggc gatttccggt tcagtcgctt tctcctgcga
    10741 cgcaacatgt gactgcatac cccagccacg ctccggttga tagagcgtcg cccagatcca
    10801 cagaaacggc cagataacat gcagagtaaa caggctcacc cagcccgccg tatgaatagc
    10861 gtcggcatgg gggtgattgc gctttttggc aatcagataa gggatgtcat gtatcgcgat
    10921 gattccataa aaaatcacca gaaagacgaa gatcagcact cccaacgcga aatagtttag
    10981 aaacatatct gcctcggatt cacgtttatc agtgttgttt tttggctggc agccagaagg
    11041 gagtcaggct gatattttga caataatccg ggttcgcgat tctcgccata acaccaaaga
    11101 ataattttta gaggtgatga gttgcttagt tacataacga ttgtatgacg aaggcataac
    11161 atgctgtaga tcacatcagg tgaacgccgt aagaaaatat cttgtgattc agatcacaaa
    11221 gattcaacaa accatcaaaa caaaaatgtg acattactca catttaaatg ccatttttag
    11281 cgaaaatcac cgccttgttt cttttttata caggcgtttt gtgatgagcg tcacgtcaat
    11341 tacctctcta ccccctatat ttatgtgact gatatcacac aaaagcccgt cgactggaca
    11401 gttaaccgat tcagtgccag atttcgcagt atctacaagg tccggctacc tctgccgcca
    11461 cattaacaaa aaacctcggg cttccagcct gcgcgacagc aaacataaga aggggtgttt
    11521 ttatgtcatc cgatattaag atcaaagtgc aaagctttgg tcgtttcctc agcaacatgg
    11581 tgatgccaaa tatcggcgcg tttatcgcgt ggggtatcat caccgcgtta tttattccaa
    11641 cagggtggtt accgaacgag acgctggcga agctggtcgg gccgatgatc acttatctcc
    11701 taccgctgct gatcggttat accggtggta agctggtagg cggcaaacgt ggtggcgtag
    11761 tcggtgccat taccaccatg ggcgttatcg tcggcgcaga catgccgatg ttcctcggtt
    11821 ctatgattgc aggtccgctg ggcggctggt gcattaagca cttcgaccgc tgggtagacg
    11881 gtaagatcaa atccggtttt gagatgctgg tgaataactt ctccgcaggc atcatcggga
    11941 tgatcctcgc tattctggca ttcctcggca ttggcccgat tgttgaagcc ctgtccaaaa
    12001 tgctggctgc gggcgttaac ttcatggttg tccatgacat gctgccgctg gcgtctatct
    12061 ttgttgaacc ggcgaaaatc ctgttcctca acaacgccat taaccacggt atcttctcgc
    12121 cgctgggtat tcagcagtcc catgaactgg gtaaatcaat cttcttcctg attgaagcta
    12181 acccaggtcc aggtatgggc gtgctgctgg cgtacatgtt ctttggtcgt ggtagcgcta
    12241 aacagtctgc gggcggtgcg gcaatcatcc acttcctggg tggtatccac gaaatctact
    12301 tcccgtatgt gctgatgaat ccgcgtctga tcctcgcagt catcctcggc ggtatgactg
    12361 gcgtgttcac gctgactatc ctgggcggtg gtctggtttc tccggcatct ccgggttcta
    12421 tccttgctgt actggcgatg acaccaaaag gtgcttattt cgctaacatc gcgggtgtgt
    12481 gtgcggcgat ggctgtctcc ttcgttgtct ctgctatttt gctgaaaacc agcaaagtga
    12541 aagaagaaga tgatattgaa gcagcaactc gtcgtatgca ggacatgaaa gctgagtcta
    12601 aaggcacatc tccgctatct gcgggcgatg tgactaacga cctgagccac gtacgtaaaa
    12661 tcatcgttgc ctgtgacgcc ggtatgggtt caagtgcgat gggcgcaggc gtgctgcgta
    12721 agaaaattca ggatgcaggt ctgtcgcaga tttcggtcac taacagcgcg atcaacaacc
    12781 tgccgccaga tgtggacctc gtcatcactc accgtgacct gaccgaacgc gctatgcgcc
    12841 aggttccgca ggcacagcat atttcgctga ccaacttcct cgacagcggc ctgtacacca
    12901 gcctaaccga acgtctcgtt gctgcccagc gccatactga aaacgaagtg aaagtgaaag
    12961 acagtctgaa agacagcttt gacgattcca gtgctaacct gttcaaactg ggcgcggaga
    13021 acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt gctggcgagc
    13081 agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg gatcgtgaaa
    13141 aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg gttgaagcga
    13201 aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc gtgcgcttcg
    13261 gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt aacaacgagc
    13321 acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc atcgagcgtc
    13381 tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt aagtaatcca
    13441 atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct
    13501 cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat
    13561 atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc
    13621 gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg
    13681 gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt
    13741 gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt
    13801 gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt
    13861 aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac cacgcagctg
    13921 aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agagcacgtt
    13981 ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat
    14041 ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa
    14101 ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa
    14161 cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc
    14221 ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt
    14281 gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat
    14341 gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta
    14401 gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca
    14461 ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt
    14521 gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct
    14581 gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa cagcgaggtt
    14641 gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca
    14701 ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggccg
    14761 ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca
    14821 ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag
    14881 acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg
    14941 atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg
    15001 aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc aacgagtacg
    15061 cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac
    15121 caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct
    15181 atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccaaaatca
    15241 gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg
    15301 gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg
    15361 gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct
    15421 ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt
    15481 tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt
    15541 ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc
    15601 tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc gtagtttttg
    15661 agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga acaaagcacc
    15721 tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag
    15781 tcgaaaaaaa cgaaatcaag cgtctcagcg atcgcctgga cgccatccgc caccagcagg
    15841 ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca
    15901 cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg agtaaagaag
    15961 cacagaagct gatgaagatg ccgttccagc gcgcaattac caaaaaagag caggctgata
    16021 tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg
    16081 ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt taagcccctc
    16141 agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt caatgcgggg
    16201 actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc gatttaccgc
    16261 tggttgatat tctgaaaatg catttttacc gaaatagcca tctataaaca ataaaaatat
    16321 atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg gatttaattg
    16381 tgaaatttta atcatcatat tcacaaaatc aattatctgt gaaatttttc ccagacatgg
    16441 tttctttcat atttgacatt tattaataat catcattaga atccacgaaa aataatatat
    16501 tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga atgcttaaaa
    16561 caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagct caccaataat
    16621 acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat ttttacatga
    16681 acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctctgcgca ttaacgggat
    16741 gtgactacat tgaagaaggc aagccagaaa gtagtttact gaaacaacaa gaagaacata
    16801 ataataaaat tgtgctactt gaaaaacaac aagcacagct aaagagtcaa ctcgaaacta
    16861 ttcaaaaaca acaaactggc ataataaaca gtacaaagac tttaactcac gtaattaaat
    16921 ccgttaaaga ccaacaaaat acttttattt ttaccgagtt taacccagca aaaacgaagt
    16981 atttcatcct gaacaacggt tcggtggctt tagcgggtcg agtgttatcc attgacgcca
    17041 cagagaatgg tagtgttatt catatctcac tggtcaactt attaagtaca cctatctcaa
    17101 acattggttt taatgcgaca tggggtggtg aaaaacctgt cgatgccaaa gagtttgccc
    17161 ggtggcaaca attacttttc aacacatcaa tgaaatccac attgaaatta ttaccaggtc
    17221 aatggcaaga cattaatttg accctgaagg gtgtatcgcc caataacctg ggatatctga
    17281 agttagccat caacatggaa aatattcagt tcgacaatct tccctctgct gaaaatcggc
    17341 agaaaagaag caaaaaataa atgcagttag tacgcttata acaaggaata gcgagcatca
    17401 ttaatgaaca aaatatttaa agttatctgg aaccctgcga cagggaatta tactgttacc
    17461 agcgaaacgg caaaaagccg tggcaagaaa tctgggcgca gtaagctgtt aatttctgcg
    17521 ctggttgcgg gtggaatgtt gtcgtcgttt ggggcattgg cgaatgccgg gaatgacaac
    17581 ggtcagggtg ttgattacgg tagtggatca gctggcgacg gctgggttgc tataggcaaa
    17641 ggggcgaaag caaatacttt tatgaacacc agtggttcca gtactgctgt gggttatgac
    17701 gctatagctg aaggccaata tagctctgcc atcgggtcaa aaacccatgc gattggtggt
    17761 gcatcaatgg cctttggggt tagtgcaata tcagaaggcg atagaagtat agcactgggt
    17821 gcctcttcgt attcattggg ccaatactca atggccctcg gccgttattc aaaagcattg
    17881 ggtaaattgt ctattgctat gggggactct tccaaagcgg aaggagcaaa cgccattgcc
    17941 ctggggcgta gcagtgtagc tagcggtact gacagcctcg catttggcag acaatcactt
    18001 gccagcgcag cgaacgctat tgcgataggt gctgagaccg aagccgctga aaatgcaact
    18061 gctattggca ataatgcgaa ggcaaaaggg actaatagca tggcaatggg gttcggaagc
    18121 cttgccgata aagtcaatac tatcgcatta ggaaatggca gccaggctct ggcagataat
    18181 gcaatcgcca taggccaggg caacaaagct gatggcgtgg atgccatcgc tctgggtaat
    18241 ggtagccagt cgagaggctt aaacaccatt gccttaggca cagccagtaa tgcaactggt
    18301 gataagagtc ttgcgcttgg tagtaatagc agtgccaacg gtattaactc tgtcgcgctg
    18361 ggcgcagatt ccattgcgga tttagacaat accgtctctg tcggcaatag ttcattaaaa
    18421 cgcaagatcg ttaatgtgaa aaatggcgcg atcaagtctg acagttacga tgccattaat
    18481 ggttcacagc tttatgccat tagcgactcg gtagcaaaaa aggcttggag gaggggctgc
    18541 agtagatgtt gatgacggta ctgttacagc accaacctac aatttaaaaa atggtagcaa
    18601 aaataacgta ggggctgcgc tcgctgtact tgatgaaaac accctgcaat gggaccaaac
    18661 caaaggcaaa tacagcgctg ctcatggtac tagtagccca actgccagcg taatcaccga
    18721 tgttgcggat ggcacgattt cagcctccag taaggatgcg gttaacggtt cccaactgaa
    18781 agctaccaat gacgatgtcg aagccaacac caccaatatc accaacctga cggattccgt
    18841 tggtgacctt caggctgatg ccctgctctg gaacgaaact aaaaaggcat tcagtgcagc
    18901 tcacggccag gataccacca gcaaaatcac caacgttaaa gatgccgacc tgacggctga
    18961 cagcactgat gctgttaacg gctctcagct gaaaaccacc aacgatgctg tggcgacgaa
    19021 taccaccaat atcgccaata acacttccaa tattgccact aacaccacca acatctctaa
    19081 cctgactgag acggtgacta atcttggtga ggatgcgctg aaatgggata aggacaatgg
    19141 tgtattcacg gcagctcatg gcaccgagac caccagcaaa ataaccaacg ttaaagatgg
    19201 cgacctgacg actggcagca ccgatgccgt taacggctct cagctgaaaa ccaccaacga
    19261 tgccgtggcg acgaatacca ccaatatcgc cactaacacc accaacatct ctaatctgac
    19321 tgagacggtg actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtctt
    19381 cactgcagct catggcaaca ataccgccag caaaatcacc aatatcctgg acggcacagt
    19441 cactgcaacc agttccgatg ccattaacgg tagccagctt tatgacttaa gcagcaatat
    19501 cgccacctac ttcggcggca atgcttctgt gaatactgac ggtgtgttta ccggtccaac
    19561 ctacaaaatc ggtgaaacaa attattataa cgtcggcgat gcactggctg cgattaactc
    19621 ctcatttagc acgtctctcg gcgatgctct gctttgggat gccaccgcag gtaaattcag
    19681 tgccaaacac ggtactaatg gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat
    19741 ttcagactcc agttctgacg cagtaaacgg ctcacaactc cacggcgtga gcagttatgt
    19801 tgttgatgcg ctggggggtg gtgccgaagt caatgcagac ggcaccatca ctgcgccgac
    19861 gtacaccatt gctaatgctg attacgataa tgtcggtgat gccctgaatg ctatcgatac
    19921 cactcctgac gacgctctgc tctgggatgc ggacgccggt gaaaatggtg catttagcgc
    19981 cgctcacgga aaagataaaa ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc
    20041 tgctgccagc agcgacgcga ttaacggctc acaactctat accaccaata agtacatcgc
    20101 tgatgcgctg ggtggtgacg cagaagtcaa cgctgacggc accatcaccg caccgactta
    20161 caccattgcg aacgccgagt acaacaacgt cggtgacgcc ctggatgcgc ttgatgataa
    20221 cgccctgctg tgggataaga ctgccaatgg cggtgctgga gcctacaatg ccagccatga
    20281 cggtaaagcc agcatcatca ctaatgtcgc taatggcagt attagtgagg acagtaccga
    20341 tgcagtgaac ggttctcagt tgaatgcgac gaatatgatg attgagcaga acacccaaat
    20401 tatcaatcag ctcgctggta acaccgacgc aacctatatc gaagaaaatg gcgctggtat
    20461 caactacgtt cgtactaacg acaacgattt agcctttaac gatgcaagcg cctctggtgt
    20521 cggcgctaca gctgtaggtt ataacgctgt cgcgtctggt gccagcagcg tagccattgg
    20581 tcagaacagc agcagcaccg ttgataccgg tattgcgctg ggtagcagct ccgtttccag
    20641 ccgtgtgatt gccaaaggtt ctcgtgacac tagcgtaacg gaaaatggcg tggtgattgg
    20701 ttatgacacc actgacggcg aactgctagg tgcattgtca attggtgatg acggtaaata
    20761 ccgccaaatc atcaacgtag ctgatggttc agaagcccat gacgccgtta cggttcgcca
    20821 gttgcagaac gctattggag cggtcgccac tacgccaacc aagtactttc acgccaactc
    20881 aacggcagaa gactcactgg ccgttggtga agactcgctg gcaatgggtg cgaaaactgt
    20941 cgttaatggt aatgcaggta ttggtatcgg tttgaacact ctggttctgg ctgatgcgat
    21001 caacggcatt gctatcggca gtaacgcacg cgcaaaccat gcaaacagta tcgcaatggg
    21061 taacggttct cagaccaccc gtggtgcaca gactggctac accgcctaca acatggacgc
    21121 accgcagaac tctgtaggtg agttttctgt cggtagtgaa gacggtcaac gtcagatcac
    21181 aaacgtcgca gctggttcag cggataccga tgcggttaac gtgggtcagt tgaaagtcac
    21241 tgatgagcgc gtagcgcaaa atacccagag cattactaac ctgaacaatc aggtcactaa
    21301 tctggatact cgcgttacta atatcgaaaa cggtattggc gacattgtca ccaccggtag
    21361 caccaagtac ttcaagacca acaccgatgg cgtagatgcc aacgcccagg gtaaagatag
    21421 cgttgctatt ggttctggtt ccattgctgc cgctgacaac agcgtcgcac tgggtaccgg
    21481 ttccgttgca gaggaagaaa atacaatctc tgtaggttct tctactaacc aacgccgtat
    21541 caccaacgta gctgccagtg ttaatgccac cgatgcggtt aacgtgtcgc agctgaaatc
    21601 ttctgaagca ggcggagtgc gctacgacac caaagctgat ggttctatcg actatagcaa
    21661 tatcaccctc ggtggtggca acggcagtac gactcgtatc agcaacgtct ccgctggcgt
    21721 caacaacaac gacgcggtga actacgcgca gttgaagcaa agcgcgcagg aaacgaagca
    21781 atacaccgat cagcggatgg ttgagatgga taacaaactg tctaaaactg aaagcaagtt
    21841 gagcggtggt atcgcttctg caatggcaat gaccggtctg ccgcaggctt atacaccggg
    21901 tgccagcatg gcttctattg gtggcggtac ttacaacggt gaatcggcag ttgctttagg
    21961 tgtatcgatg gtgagcgcca atggtcgttg ggtctacaaa ttacaaggta gtaccaatag
    22021 ccagggtgaa tactccgccg cactcggtgc cggtattcag tggtaatgat ccattaagtt
    22081 agtgtgacta agggcaaacg ccaaattgcc tgatgcgtta cgcgcatcag gcaagataag
    22141 gcgctccggg tcaacaatct aagggcaatt ctctgatgag gattgccctt ttctttacca
    22201 gacatctccc cccacaagaa ttggccctac caattcttcg cttatctgac ctctggttca
    22261 caattcccca attaaaactc acatcaatat tgccaataca taacatttag ttaaccattc
    22321 attgtcatta tccctacaca acacaattgg cagtgccact tttacacaac gtgtgacaag
    22381 gagatgagca acagactcat tacacgatgt gcgtggactc taggagacct gcaatgaatc
    22441 tctggcaaca aaactacgat cccgccggga atatctggct ttccagtctg atagcatcgc
    22501 ttcccatcct gtttttcttc tttgcgctga ttaagctcaa actgaaagga tacgtcgccg
    22561 cctcatggac ggtagcaatc gcccttgccg tggctttgct gttctataaa atgccggtcg
    22621 ctaacgcgct ggcctcggtg gtttatggct tcttctatgg tttgtggccc atcgcgtgga
    22681 tcattattgc agcagtgttc gtctataaga tctcggtgaa aaccgggcag tttgacatca
    22741 ttcgctcgtc tattctttcg ataacccctg accagcgtct gcaaatgctg atcgtcggtt
    22801 tctgtttcgg cgctttcctt gaaggagccg caggctttgg cgcaccggta gcaattaccg
    22861 ccgcattgct ggtcggcctg ggttttaaac cgctgtacgc cgccggactg tgcctgattg
    22921 ttaacaccgc gccagtggca tttggcgcga tgggcattcc aatcctggtt gccggacaag
    22981 taacaggcat cgacagcttt gagattggcc agatggtggg gcgtcagcta ccgtttatga
    23041 ccattatcgt gctgttctgg atcatggcga ttatggacgg ctggcgcggt atcaaagaga
    23101 cgtggcctgc ggtcgtggtt gcaggcggct cgtttgccat cgctcagtac cttagctcta
    23161 acttcattgg gccggagctg ccggacatta tctcttcgct ggtatcactg ctctgcctga
    23221 cgctgttcct caaacgctgg caaccagttc gcgtattccg cttcggtgac ttgggggcgt
    23281 cacaggttga tatgacgctg gcccacaccg gttacaccgc gggtcaggtg ttacgtgcct
    23341 ggacaccgtt cctgttcctg accgctaccg taacactgtg gagcatcccg ccgtttaaag
    23401 ccctgttcgc atcgggtggt gcgctgtatg agtgggtgat caacattccg gtgccgtacc
    23461 tcaataaact ggttgcccgt atgccaccag tggtcagcga ggctacagcc tatgccgccg
    23521 tgtttaagtt tgactggttc tctgccaccg gcaccgccat tctgtttgct gcactgctct
    23581 cgattgtctg gctgaagatg aaaccgtctg acgctatcag caccttcggc agcacgctga
    23641 aagaactggc tctacccatc tactccatcg gcatggtgct ggcgttcgcc tttatctcga
    23701 actattccgg actgtcatca acactggcac tggcactggc gcacaccggt catgcattca
    23761 ccttcttctc gccgttcctc ggctggctgg gggtattcct gaccgggtcg gatacctcat
    23821 ctaacgccct gttcgccgcg ctgcaagcca ccgcagcaca acaaattggc gtctctgatc
    23881 tgttgctggt tgccgccaat accaccggtg gcgtcaccgg taagatgatc tccccgcaat
    23941 ctatcgctat cgcctgtgcg gcggtaggcc tggtgggcaa agagtctgat ttgttccgct
    24001 ttactgtcaa acacagcctg atcttcacct gtatggtggg cgtgatcacc acgcttcagg
    24061 cttatgtctt aacgtggatg attccttaat gattgtttta cccagacgcc tgtcagacga
    24121 ggttgccgat cgtgtgcggg cgctgattga tgaaaaaaac ctggaagcgg gcatgaagtt
    24181 gcccgctgag cgccaactgg cgatgcaact cggcgtatca cgtaattcac tacgcgaggc
    24241 gctggcaaaa ctggtgagcg aaggcgtgct gctcagtcga cgcggcggcg ggacgtttat
    24301 tcgctggcgt catgacacat ggtcggagca aaacatcgtc cagccgctaa aaacgctgat
    24361 ggccgatgat ccggattaca gtttcgatat tctggaagcc cgctacgcca ttgaagccag
    24421 caccgcatgg catgcggcaa tgcgcgccac acctggcgac aaagaaaaga ttcagctttg
    24481 ctttgaagca acgctaagtg aagacccgga tatcgcctca caagcggacg ttcgttttca
    24541 tctggcgatt gccgaagcct cacataacat cgtgctgctg caaaccatgc gcggtttctt
    24601 cgatgtcctg caatcctcag tgaagcatag ccgtcagcgg atgtatctgg tgccaccggt
    24661 tttttcacaa ctgaccgaac aacatcaggc tgtcattgac gccatttttg ctggtgatgc
    24721 cgacggggcg cgtaaagcaa tgatggcgca ccttagtttt gttcacacca ccatgaaacg
    24781 attcgatgaa gatcaggctc gccacgcacg gattacccgc ctgcccggtg agcataatga
    24841 gcattcgagg gagaaaaacg catgattatt tccgcagcca gcgattatcg cgccgcagcg
    24901 caacgcattc tgccgccgtt cctgttccac tatatggatg gtggtgcata ttctgaatac
    24961 acgctgcgcc gcaacgtgga agatttgtca gaagtggcac tgcgccagcg tattctgaaa
    25021 aacatgtccg acttaagcct ggaaacgacg ctgtttaatg agaaattgtc gatgccggta
    25081 gcactggcac cggtgggttt gtgtggcatg tatgcgcgtc gtggcgaagt tcaggcagcc
    25141 aaagcggcgg acgcgcatgg cattccgttt actctctcga cggtttccgt ttgcccgatt
    25201 gaagaagtcg cgccagccat caagcgccca atgtggttcc agctttatgt actgcgcgat
    25261 cgcggcttta tgcgtaacgc gctggagcga gcaaaagcag cgggttgttc gacgctggtt
    25321 ttcaccgtgg atatgccgac accgggcgca cgctaccgtg atgcgcattc aggtatgagc
    25381 ggcccaaatg cggcaatgcg ccgctacttg caggcggtga cgcatccgca atgggcgtgg
    25441 gatgtgggcc tgaacggtcg tccacatgat ttaggtaata tctcagctta tctcggcaaa
    25501 ccgaccggac tggaagatta catcggctgg ctggggaata acttcgatcc gtccatctca
    25561 tggaaagacc ttgagtggat ccgcgatttc tgggatggcc cgatggtgat caaagggatc
    25621 ctcgatccgg aagatgcgcg cgatgcagta cgttttggtg ctgatggaat tgtggtttct
    25681 aaccacggtg gccgccagct ggacggtgta ctctcttccg ctcgtgcgtt gcccgctatt
    25741 gcagatgcgg tgaaaggtga tatcgccatt ctggcggata gcggaattcg taacgggctt
    25801 gatgtcgtgc gtatgattgc gctcggtgcc gacaccgtac tgctgggtcg tgctttcctg
    25861 tatgcgctgg caacagcggg ccaggcgggt gtagctaacc tgctaaatct gatcgaaaaa
    25921 gagatgaaag tggcgatgac gctgactggc gcgaaatcga tcagtgaaat tacgcaagat
    25981 tcgctggtgc aggggctggg taaagagttg cctacggcac tggctccgat ggcgaaaggg
    26041 aatgcggcat agtcgtttgc gccccctcac cctaaccctc tccctcaggg agaggggacc
    26101 gttcggcgct gtatgtactc cctcactctg aaacgacacc gcactctttt tttctccctc
    26161 gcccctccgg ggagggccgg ggtgagggga aaaggccgca cggttcacca attttctgct
    26221 atcctgcccc cgcactaagg gggcagtatg ctaaacatcg tactttacga accagaaatt
    26281 ccgccaaata ctggcaacat catccgtctt tgcgctaata ccggctttcg tctacatatc
    26341 atcgaaccga tgggatttgc ctgggacgac aagcgcctgc gccgcgcggg gctggactat
    26401 cacgagttta ccgccgttac gcgtcatcat gactatcgcg cgttcctcga agcagaaaat
    26461 ccccagcgcc tgtttgccct caccacgaaa ggtacgcctg ctcacagcgc cgtaagctat
    26521 caggatggcg attatctgat gttcggcccg gaaacacgcg gcctgccagc gagcattctt
    26581 gatgccctgc ccgctgaaca aaaaattcgc attccgatgg tgccggacag ccgcagcatg
    26641 aatctgtcca atgcggtgtc ggtagtggtg tatgaagcct ggcggcagct gggatatccg
    26701 ggagcgttgt tgagagatta gttactgtat gccggataga tgattacatc gcatccggca
    26761 cgatcacagg acattagatc ccatccccat actcaaatgt atggttaata ccgttgaaat
    26821 gctgatccat atccattgat ggcttatcgc tgtctggttt accgacaata cgaaccggaa
    26881 cgccagcggc ggttgtatgc ggcggcaccg gttgcagcac cacggaacct gcgccaatct
    26941 tcgcgccgcg accaacttca atattgccga ggattttcgc gcccgcgcca atcatcacac
    27001 cttcacgaat tttcgggtga cggtcaccac cagatttacc cgtaccgcca agcgtcacag
    27061 attgcagaat cgatacgtcg ttttcaatta ccgccgtttc acccacaacg atgcccgtgg
    27121 cgtggtcgag cataatgcca cggccaattt ttgctgccgg gtgaatatcg acctggaacg
    27181 tcacagaaac ctggttttgc agaaagattg ccaatgcgcg acgcccctga ttccacaacc
    27241 agtgaccgat gcgataggcc tgcaaggcat gaaaaccctt caggtataac aacggggttg
    27301 agtatttatc gactgccggg tcgcgggtac gcaccgcctg aatatcacag gccgcagagg
    27361 cgatcatttc cgggtcagcg gcgtaggctt cttcaaccac ttcacggata gcaatagcag
    27421 gcataattgg cgatgacagc ttgttcgcca gcatgtagct cagtgcactg ccaaggtttt
    27481 cgtgcttgag tagcgtcgcg tggtaaaaac tggccagcat tggctcacag tccgccagcg
    27541 ttctggcttc ggctttaata ttgttccaga caatttccag ttcttcacac gacattgctt
    27601 actccacacg atgagataat gaccggcccg ttctgcgcgg gccgggtcat agcggtaaca
    27661 aaggttccct ggggttagtg actgctgcgc tcgtccttgc gtgcacgacc taataatgtc
    27721 aatgctgcct cgcgcgcgtt ttttccgcaa tataatactt gataaatttc ctcggttatt
    27781 ggcatttcaa cgccgaagcg atgcgccagt tcgcggactt ctttcgtatt gcggtagcct
    27841 tccaccacct gaccaatctt ctcctgcgcg ctttgtacat ccatgccctg accgagcatc
    27901 ataccaaaac ggcggttacg cgactggttg tcggtacagg taagcaccag atcgcccagc
    27961 cccgccatgc ccataaaggt ggcaggatcg gcacccagcg ccgcaccaag acgcgacatt
    28021 tcagccagcc cacgggtgat cagcgccgta cgcgcattcg caccaaaacc gataccgtca
    28081 gacatccccg cgccaatggc aatgacgttt ttcaccgcgc cgccaagctg cacgccaatg
    28141 aaatccgggt tgctgtaaac gcggaaactt ttgccgcagt gcagcagctg ctggagatca
    28201 tcggcaaagg tctgatcggt cgaggccagc gaaatagctg tcggtaaacc tgccgccagt
    28261 tctttcgcaa acgttgggcc agagataacc gccagcggaa tttgatcgcc taaggcctca
    28321 cgcgccacgt cctgtaacag acgtccggtt tccgcttcca gccctttggt cgcccacacc
    28381 agacgcgcat caggacgcat cagcggttta atctggcgca gcacttcacc aaagacatgg
    28441 ctgggtacga cgacgagaat attacgactg gctgccagcg cagtggcgag atcgctttca
    28501 agatggagcg tatcgggaaa aggcacatcg gggagaaacg cggcgttaca gcggtcgcgt
    28561 tcaagcgttg cgatatgttc agggtcatgg ccccagagga caacctcgtg gccatttctt
    28621 gccagggtaa tggcaagagc ggtgccgtac gagccggcac cgatcacagt cattgaagca
    28681 ttacgttggt tcatcaggca tcctgatgtt cttcagtacc ttcgccagcc tgctgctgca
    28741 aatagttcat gaacagcgca tcgaagttaa ccggcgcaag gttcagttgc gggaatgtac
    28801 cgcgggatac catgctggtg atgcactcac gagcatacgg gaacagaatg ttcgggcagt
    28861 atgcgcccag gcaatgtgcc atctgggtgc cttcgatacc cgcgatggag aaaataccgc
    28921 cctgctgaac ttcacacagg aacgcggttt cttcgcccaa ggaggccgtt acggtaacac
    28981 gcagtaccac ttcgtatacg tcatctgcca gttgggaaga tgccgtatcc agatcaagtt
    29041 taacttctgg ttgccaatct ttctggaaaa cgtgcggcgc gttcggcgct tcgaaagaga
    29101 tatccttggt ataaatacgt tggatctgga aagtcatttc agtgttgttt tgttctgaca
    29161 tgtgtagaaa acccttaagt gttgtcctta aatactgcgc aatgtaatac caacgttcac
    29221 acgttatttc agcaggggat ccagtccacc acgtgcatcc aatgcataca agtcatcaca
    29281 gccgccaatg tgctgtgcgt caataaaaat ctggggaacc gtggtgcaac cgctgcgttt
    29341 gatcatctct tcacgcttgg cggcattgcc atcgatcggc aactcctgga aactcacgcc
    29401 cttgctgctc agcagtgctt ttgcacgatg gcaatacggg caggtttctt tggtatagat
    29461 ttcaacattg gccataattt agctcctgtt tttttaccct gaaatttcat gttgcagggc
    29521 ggctgcaaaa tggagtgtcc ccagaagctt ataacaataa gtcgctgggg gcctaattgc
    29581 agctaacggc ctgcatcatg aaagacgaca ggtaaattat ttgccgcgca ccagaggcaa
    29641 gttttcgcca gcccagccag caacgccctc tttcagcacg aatacttgcg caaaaccagc
    29701 tttcgtcagc gcgtttgcag gctcctggca ctgcatgcca gaaccgtcta ccacgataac
    29761 cgatttgtct ttgtgcttct caagctcacc aacattgttg gctttgattt cgctcggcaa
    29821 cagattgata gaacctgcga tatggccttt acggaagtca tcacgctgac gtaaatccac
    29881 aaccacagcg tcttctttgt tgatcagacg cgtagcttca ccacgagtaa tcaccttcac
    29941 tttcgaggtc aggctcttaa acgtagtcac aagaaccgcc accagtaacg cgatccaggc
    30001 gatactcagt atgggatgac ggccaacaaa ttgcataatt tcttgcatgg ggggtaacaa
    30061 ctcccgacgt agtgattaaa aaaaccagga aaggagtata cctgcgcggt gcggcaaata
    30121 cagccagcgc gttaactgga atgcaatttt gcggggcacg acgaaaaaaa agcgcaaatc
    30181 tggcatcggc ttgccgcgag cggtcgtata ttttgatctt cagaggctat tttatcgatt
    30241 cagctgtagt aaaattacgc aaattttgac tcttgagtat gaggttgtcg caatgtcggt
    30301 ttctaaaaaa cctatggtac tggtgattct ggatggctat ggctatcgtg aagaacagca
    30361 ggataacgcc atttttagtg ctaaaacccc ggtaatggat gcactgtggg ccaatcgtcc
    30421 gcataccctg atcgacgctt ccggtctgga agtcggtctg cctgaccgtc agatgggtaa
    30481 ctccgaagta ggccacgtta acctgggtgc cggccgcatc gtgtatcagg acctgactcg
    30541 tctggacgtt gaaatcaaag atcgcgcttt ctttgctaat ccagtgctgg ctggcgcggt
    30601 acataaagcg aaaaacgcag gtaaagcagt acacattatg ggtctgctct ccgcaggcgg
    30661 cgtacacagc cacgaagatc acatcatggc gatggtagaa ctggcagctg aacgcggcgc
    30721 agaaaaaatc tacctgcacg cattccttga cggtcgcgac accccgccgc gcagtgctga
    30781 atcctcgctg aaaaaatttg aagagaaatt tgctgcgctg ggtaaaggcc gcgtagcgtc
    30841 catcattggt cgttactacg cgatggaccg cgataaccgt tgggatcgcg ttgaaaaagc
    30901 ctatgacctg ctgactctgg cgcagggtga gttccaggcc gatactgccg ttgctggttt
    30961 gcaggcagct tatgctcgcg acgaaaacga cgagttcgtg aaagcgaccg ttattcgtgc
    31021 agaaggtcag ccagatgcgg caatggaaga cggcgatgcg ctgattttca tgaacttccg
    31081 tgctgaccgc gcgcgtgaaa tcactcgtgc attcgtgaac gctgatttcg atggcttcgc
    31141 gcgtaagaaa gtggttaacg tcgatttcgt gatgctgacc gaatacgccg ctgacatcaa
    31201 aactgcggtt gcttacccac ccgcttccct ggttaacacc ttcggcgagt ggatggcgaa
    31261 aaacgacaaa actcagttgc gtatttccga aaccgaaaaa tatgcccacg ttactttctt
    31321 cttcaacggt ggcgtagaag agtcgttcaa aggcgaagat cgcattctga tcaactcacc
    31381 gaaagtggct acctacgatc tgcaaccgga aatgagctcc gcagagctga ccgaaaaact
    31441 ggttgcggcc atcaagagcg gcaaatacga caccattatc tgtaactatc cgaacggcga
    31501 catggtaggt cacaccgggg tgatggaagc ggcggttaaa gcggttgaag cactggatca
    31561 ctgcgtggaa gaagtcgcga aagcggttga gtccgtgggt ggacaactgc tgatcaccgc
    31621 tgaccacggt aacgctgaac agatgcgcga tccggcaacg ggtcaggcac acacggcaca
    31681 caccaacctg ccagttccgc tgatttacgt tggtgataag aacgtgaaag cggttgcagg
    31741 cggcaaactt tctgacatcg cgccgaccat gttgtcgctg atgggtatgg aaatcccgca
    31801 agagatgact ggtaagccgc tgttcatcgt ggaataatcc ctccccatga ggggaaaggc
    31861 gattaatacc atgacacggg ccgtgaaacc gcgcaggttt gcaatcaggc ccatcatcta
    31921 cgccagcgtt cttagcgctg gcgtattgtt gtgcgccttt tccgcccacg cggatgagcg
    31981 tgaccaactc aaatctattc aggccgatat cgccgcaaaa gagcgcgcgg tacgccaaaa
    32041 gcaacaacaa cgcgcaagcc tgctcgcaca attgaaaaag caggaagaag cgatctctga
    32101 agccacccgt aagctgcgcg aaacgcaaaa cacgctcaat caactcaata aacagattga
    32161 tgagatgaac gcgtcgattg ccaaactgga gcagcaaaaa gccgcccagg agcgcagcct
    32221 cgccgcacaa ctggatgccg cattccgtca gggcgagcat accggtattc agctaattct
    32281 cagcggtgaa gaaagccagc gtggacagcg tttacaggct tatttcggct atctcaacca
    32341 ggcgcgacaa gaaaccattg cccagttgaa gcaaacgcgt gaagaagtca ccatgcagcg
    32401 tgctgaactg gaagagaaac aaagcgagca acaaacgctg ttatatgaac agcgcgccca
    32461 acaggcgaaa ctgactcagg ctctgaacga gcgtaaaaag acgctggcgg ggctggaatc
    32521 ttccatccag caaggtcagc agcagctaag tgaactgcgt gccaacgaat cccgtctgcg
    32581 taacagcatt gcccgtgcgg aagccgcggc gaaagcgcgt gctgaacgag aagcgcgtga
    32641 ggcccaggcg gttcgcgagc gccagaaaga agcgacgcgc aaaggcacca cctacaaacc
    32701 gaccgaaagc gaaaaatcgc tgatgtcccg aactggtggc ctgggtgcgc cgcgtggtca
    32761 ggcattctgg ccggttcgcg ggccgacgct gcatcgctat ggcgagcagc tacagggtga
    32821 attacgctgg aaagggatgg ttatcggtgc ctctgaaggt actgaagtta aagcaattgc
    32881 cgacggtcga gtgattctgg ctgactggct gcaaggttac ggtctggtgg tggtggttga
    32941 gcacggtaaa ggcgacatga gtctttacgg ctataatcag agcgcactgg tgagcgttgg
    33001 ttcgcaggtt cgcgcgggcc agccaattgc actggtgggc agcagtggcg gtcagggtcg
    33061 gccttcactc tatttcgaaa ttcgccgcca gggtcaggcg gtcaatccac agccgtggtt
    33121 gggaagataa gttttgtttc catttcgtcg taacgttctt gcatttgccg ctctgttggc
    33181 gctctcctcc cccgtacttg ctggcaaact tgccatcgtc attgatgatt ttgggtatcg
    33241 cccgcacaac gaaaaccagg tgctggcgat gccttccgct atctccgtcg ctgtattacc
    33301 cgattcaccg cacgccagag agatggcgac caaagcgcat aacagcggtc acgaagtgtt
    33361 gattcatctc ccgatggcac cgttgagtaa acagccgctg gagaaaaata cgctacgccc
    33421 ggagatgagc agcgacgaaa ttgagcgcat tattcgtagt gcggtcaata acgtgcccta
    33481 tgccgtgggg atcaacaacc acatgggcag caagatgacc tctaacctgt ttggtatgca
    33541 gaaagtgatg caggcgctgg agcgttacaa tctttacttc ctcgacagcg taaccatcgg
    33601 taatacccag gcgatgcgcg ccgcgcaagg tactggcgtg aaggtgatca aacggaaggt
    33661 gttcctcgac gattcgcaga acgaagcgga catccgtgtg caatttaatc gcgcaattga
    33721 tctggcgcgt cgcaacggtt caaccattgc cattgggcat cctcaccctt caacggtacg
    33781 cgtgctacaa cagatggttt ataacctgcc gccagacatt acgctggtga aagccagcag
    33841 cttgctgaat gaaccgcagg ttgatacttc tacaccgccg aaaaacgctg tgcctgatgc
    33901 accgcgtaat ccgttccgtg gcgtgaagct gtgcaaaccg aagaaaccga tagaacctgt
    33961 ttatgctaat cgcttctttg aagtattaag cgaaagcatc agccagagca cgctgatcgt
    34021 ttacttccag catcagtggc aaggctgggg caaacagccc gaagcggcga agtttaacgc
    34081 tagcgcaaat taaaggcgct acgcgtaatg cgatggtgcg accgcgtttt gtcgcgccat
    34141 tgccataagc ggaaagacca cagtaacgcc tggtaaccga ccttcacgct gcgcacattg
    34201 gtaatcaggc gcttatacat acccggaagt aaatatctcg gcaatcatcc gttggcgggt
    34261 aagaatatcc ggctctttgc g
//
LOCUS       DAOFMX010000042        34261 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.042, whole genome shotgun sequence.
ACCESSION   DAOFMX010000042 DAOFMX010000000
VERSION     DAOFMX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.042"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..331)
                     /locus_tag="QBA20_002225"
     CDS             complement(<1..331)
                     /locus_tag="QBA20_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292749.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="QBA20_002226"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="QBA20_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDQ6292750.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="QBA20_002227"
     CDS             complement(1692..2072)
                     /locus_tag="QBA20_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HDQ6292751.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..2502)
                     /locus_tag="QBA20_002228"
     CDS             complement(2086..2502)
                     /locus_tag="QBA20_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292752.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="QBA20_002229"
     CDS             complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="QBA20_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDQ6292753.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3503..3970)
                     /locus_tag="QBA20_002230"
     CDS             complement(3503..3970)
                     /locus_tag="QBA20_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDQ6292754.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4135..5004
                     /gene="pldA"
                     /locus_tag="QBA20_002231"
     CDS             4135..5004
                     /gene="pldA"
                     /locus_tag="QBA20_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDQ6292755.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDASAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5137..6966
                     /gene="recQ"
                     /locus_tag="QBA20_002232"
     CDS             5137..6966
                     /gene="recQ"
                     /locus_tag="QBA20_002232"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDQ6292756.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7030..7650
                     /gene="rhtC"
                     /locus_tag="QBA20_002233"
     CDS             7030..7650
                     /gene="rhtC"
                     /locus_tag="QBA20_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDQ6292757.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="QBA20_002234"
     CDS             complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="QBA20_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDQ6292758.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8443..9465
                     /gene="pldB"
                     /locus_tag="QBA20_002235"
     CDS             8443..9465
                     /gene="pldB"
                     /locus_tag="QBA20_002235"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDQ6292759.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9473..10273
                     /gene="yigL"
                     /locus_tag="QBA20_002236"
     CDS             9473..10273
                     /gene="yigL"
                     /locus_tag="QBA20_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDQ6292760.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10349..11248
                     /gene="bioP"
                     /locus_tag="QBA20_002237"
     CDS             10349..11248
                     /gene="bioP"
                     /locus_tag="QBA20_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDQ6292761.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11136..12089)
                     /gene="metR"
                     /locus_tag="QBA20_002238"
     CDS             complement(11136..12089)
                     /gene="metR"
                     /locus_tag="QBA20_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDQ6292762.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12325..14586
                     /gene="metE"
                     /locus_tag="QBA20_002239"
     CDS             12325..14586
                     /gene="metE"
                     /locus_tag="QBA20_002239"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDQ6292763.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14625..15440)
                     /locus_tag="QBA20_002240"
     CDS             complement(14625..15440)
                     /locus_tag="QBA20_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDQ6292764.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15702..16463
                     /gene="udp"
                     /locus_tag="QBA20_002241"
     CDS             15702..16463
                     /gene="udp"
                     /locus_tag="QBA20_002241"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDQ6292765.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16604..18031
                     /gene="rmuC"
                     /locus_tag="QBA20_002242"
     CDS             16604..18031
                     /gene="rmuC"
                     /locus_tag="QBA20_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDQ6292766.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18126..18881
                     /gene="ubiE"
                     /locus_tag="QBA20_002243"
     CDS             18126..18881
                     /gene="ubiE"
                     /locus_tag="QBA20_002243"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDQ6292767.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18895..19500
                     /gene="ubiJ"
                     /locus_tag="QBA20_002244"
     CDS             18895..19500
                     /gene="ubiJ"
                     /locus_tag="QBA20_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDQ6292768.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19497..21137
                     /gene="ubiB"
                     /locus_tag="QBA20_002245"
     CDS             19497..21137
                     /gene="ubiB"
                     /locus_tag="QBA20_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDQ6292769.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEARFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21216..21485
                     /gene="tatA"
                     /locus_tag="QBA20_002246"
     CDS             21216..21485
                     /gene="tatA"
                     /locus_tag="QBA20_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDQ6292770.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21489..22004
                     /gene="tatB"
                     /locus_tag="QBA20_002247"
     CDS             21489..22004
                     /gene="tatB"
                     /locus_tag="QBA20_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDQ6292771.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22007..22783
                     /gene="tatC"
                     /locus_tag="QBA20_002248"
     CDS             22007..22783
                     /gene="tatC"
                     /locus_tag="QBA20_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDQ6292772.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22825..23607
                     /gene="tatD"
                     /locus_tag="QBA20_002249"
     CDS             22825..23607
                     /gene="tatD"
                     /locus_tag="QBA20_002249"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDQ6292773.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="QBA20_002250"
     CDS             complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="QBA20_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDQ6292774.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24259..25752
                     /gene="ubiD"
                     /locus_tag="QBA20_002251"
     CDS             24259..25752
                     /gene="ubiD"
                     /locus_tag="QBA20_002251"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDQ6292775.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25798..26499
                     /gene="fre"
                     /locus_tag="QBA20_002252"
     CDS             25798..26499
                     /gene="fre"
                     /locus_tag="QBA20_002252"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDQ6292776.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="QBA20_002253"
     CDS             complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="QBA20_002253"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDQ6292777.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="QBA20_002254"
     CDS             complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="QBA20_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDQ6292778.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30243..31574
                     /gene="pepQ"
                     /locus_tag="QBA20_002255"
     CDS             30243..31574
                     /gene="pepQ"
                     /locus_tag="QBA20_002255"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDQ6292779.1"
                     /translation="MESLASLYKNHIATLQERTRDALTRFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31574..32188
                     /locus_tag="QBA20_002256"
     CDS             31574..32188
                     /locus_tag="QBA20_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDQ6292780.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32227..33678
                     /gene="trkH"
                     /locus_tag="QBA20_002257"
     CDS             32227..33678
                     /gene="trkH"
                     /locus_tag="QBA20_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDQ6292781.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33690..34235
                     /gene="hemG"
                     /locus_tag="QBA20_002258"
     CDS             33690..34235
                     /gene="hemG"
                     /locus_tag="QBA20_002258"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDQ6292782.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8051 a         8654 c         9261 g         8295 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctagctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgttgatga cgagataatg gagtgcgatg gtagagacta taacaagaaa
     2341 acctgcttcc ccatcgttaa aaaagataat aagaaaggca aaaatgaaat ttattaaaat
     2401 aaataaaaat atatatcggt gtctggaaat cttaccgtta gatgttggga taaacatacg
     2461 taacataaat tttacatcct tgtgtgagtc tcctgtcagc atggcaatat gcccactctc
     2521 atacaagagt cggcatattt tttcagaata tatttatttt ttatttggac gttctacgct
     2581 gcgtataaat cgcgtccatc acaaaaattg ccagcgccac ccaaataaag gtgaaagtca
     2641 ccatcttatc ggcacccggt ttttcaccat aaaacgtcac agccagcagg aacatcagcg
     2701 tcgggccaat gtactgaaaa aagcctaacg ttgagagacg caggcgcgtg gcagcggcag
     2761 taaaacacaa cagcggtacg gtagtgacaa taccggcggc gatcagcagt aaattcagcg
     2821 acatcgggtt ttgccccata tggctggttg aactgtcggc aatagcaaac aggtaaatcg
     2881 ccgccacggg cagcagccac atggtttcaa ttaacatgcc ggtttgtgct tctaccgcaa
     2941 tcttcttgcg caccaggccg tagaaggcaa aactaaatgc cagccccagc gcgataatag
     3001 gtagcgaacc aaaagtccac agctggacca acacgccaca tatcgccaga atcaccgcca
     3061 gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca atgttcacca
     3121 gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc accgcccaga
     3181 taaacagtag ccagttgcca ccaatcagta cggcagagac tgccagcata aaaattttct
     3241 gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc atcagcacca
     3301 ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg ggcacgtagt
     3361 aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca agagcgagta
     3421 atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa ttggtaacag
     3481 cagtttacct gcttttatgt cttcaaccta ccatataggt ggcagtggca ctggcaatat
     3541 aaagctgttc ttcattgtgt aattcaacgc gggcgacggc gactttattg cctgcacgca
     3601 acaggctact ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa
     3661 gatcaatggt ccccatccgc gatagccgct ggcgtagttc atcttcactg atggtttcgt
     3721 ggcgggttaa ggtacttccc acgcacacca gaccggcggc gacatccagc gccgacgcaa
     3781 tgactccgcc gtgcaaaatg ctttgcgccc agttacccac catcatcggc tgatttttaa
     3841 aggccagctg tgcgaactct ttttcgtaac gctccagttc catccccaat gcgcggttaa
     3901 atggcatgtg ataaacaaac atctcaccca ctaatttcag ggcttgttca gcggtcagta
     3961 cggcagacat atcatcctta cacttcattg gttaatgaaa tgttgatttt atgcttcttt
     4021 gttgttggtt tctactttag gaagggataa ctaacggcaa tggagttaag tatgtaaaat
     4081 agcccgcaga aaaacattca ccttatcaat aattcgttac ggagaacacg accgatgcgg
     4141 actctgcagg gctggttgtt gccggtgttt atgttgccta tggcagtata tgcacaagag
     4201 gcaacggtga aagaggtgca tgacgcgtca gcggtgcgtg gcagtattat cgccaatatg
     4261 ctgcaggagc atgacaatcc gttcacgctc tatccttatg acaccaacta cctcatctac
     4321 acccaaacca gcgatctgaa taaagaagcg attgccagtt acgactgggc ggaaaatgcg
     4381 cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc cgctgtggcg tgggatttta
     4441 ggcccaaact cggtgttggg tgcgtcttat acgcaaaaat cctggtggca actgtccaat
     4501 agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt
     4561 gccaccgatt accgttttgc aggttggacg ctgcgcgatg tggagatggg gtataaccac
     4621 gactctaacg ggcgttcaga cccgacctcc cgcagctgga accgccttta tactcgcctg
     4681 atggcagaaa acggtaactg gctggtagaa gtgaagccgt ggtatgtggt gggtaatact
     4741 gacgataacc cggatatcac caaatatatg ggttactacc agcttaaaat cggctatcac
     4801 ctcggtgatg cggtgctcag tgcgaaagga cagtacaact ggaacaccgg ctacggcggc
     4861 gcggagttag gcttaagtta cccgatcacc aaacatgtgc gcctttatac tcaggtttac
     4921 agcggctatg gcgaatcgct catcgactat aacttcaacc agacccgtgt cggtgtaggg
     4981 gttatgctaa acgacttgtt ttgatgaacg gttgagtggt tggcaaatct ggaatccagc
     5041 atccaggatt accctctcag agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa
     5101 tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg cgcaggcgga agtgttgaat
     5161 ctggagtccg gagctaaaca ggttttacaa gaaacctttg gctaccaaca gtttcgcccc
     5221 ggccaggaag aaattatcga cactgtgctt tccggtcgcg attgcctggt tgttatgccc
     5281 actggtggcg gaaaatccct ttgctatcaa attcctgcct tattgctaaa cggccttacc
     5341 gtggttgttt caccgctgat ttcgttgatg aaagatcagg tggatcaact acaggccaac
     5401 ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg aacagcaact tgaagtgatg
     5461 acaggctgcc gcaccgggca aattcgctta ctgtatatcg ccccggaacg cctgatgctg
     5521 gataactttc ttgagcatct ggcgcactgg aatccggtgt tattagccgt cgatgaagcg
     5581 cactgtatct cccaatgggg ccacgatttc cgcccggaat atgccgcgct cggtcagttg
     5641 cgccagcggt tcccgacgct gccgtttatg gcgctgaccg ccacagccga cgacaccacg
     5701 cgccaggata tcgtgcgcct gctggggctg aacgatccgc tgattcaaat cagcagtttt
     5761 gaccgtccga atattcgcta catgctgatg gagaaattca aaccgctcga tcagttgatg
     5821 cgctacgtgc aggaacagcg cggtaagtct ggcattatct actgcaacag ccgggcgaaa
     5881 gtagaagaca ccgctgcgcg cctgcaaagc aggggtatta gcgcggcggc ctatcatgcc
     5941 gggctggaaa ataatgtccg cgccgacgtg caggagaaat tccagcgcga tgatctgcaa
     6001 attgtggtgg cgacggtggc gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg
     6061 gtccactttg atattccgcg caatatcgaa tcctattatc aggaaaccgg tcgcgccggg
     6121 cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc cggctgatat ggcgtggctg
     6181 cgccgttgtc tggaagagaa gccgcagggg cagttgcagg atatcgagcg ccacaaactc
     6241 aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc gtctggtatt gctgaactat
     6301 tttggcgaag ggcgtcagga gccgtgcggg aactgcgata tctgcctcga tccgccgaaa
     6361 cagtacgacg gttcaaccga tgctcagatt gccctttcca ccattggtcg tgtgaatcag
     6421 cggtttggga tgggttatgt ggtggaagtg attcgtggtg ctaataacca gcgtatccgc
     6481 gactatggtc atgacaaact gaaagtctat ggtatgggcc gtgataaaag ccatgaacat
     6541 tgggtgagcg tgatccgcca gctgattcat ctcggcctgg tgacgcaaaa tattgcccag
     6601 cattctgccc tacaactgac agaggccgcg cgcccggtgc tgcgcggcga atcctctttg
     6661 caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc
     6721 ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat
     6781 gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa ccttgattga gatggctgaa
     6841 cagatgccga tcaccgccag cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg
     6901 gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg cgacgacgaa
     6961 gagtagtcag cagcataaaa aagtgccagt atgaagactc cgtaaacgtt tcccccgcga
     7021 gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg tgcacattgt ggcgcttatg
     7081 agccccggtc ccgatttctt ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa
     7141 gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg tttgggctgg gattgcgttg
     7201 cttggcctgc atttgattat cgaaaaaatg gcctggctgc atacgctgat tatggtaggc
     7261 ggtggcctgt atctctgctg gatgggttac cagatgctac gcggtgcact gaaaaaagag
     7321 gctgtatctg cgcctgcacc acaggttgag ctggcgaaaa gcgggcgcag tttcctgaaa
     7381 ggcttactga ccaatctcgc caatccgaaa gcgattatct attttggttc ggtattctca
     7441 ttgtttgtcg gcgataacgt tggcactacc gagcgctggg gcattttcgc gttgattatt
     7501 gtcgaaacac tggcgtggtt taccgtcgtt gccagtctgt ttgccctgcc gcaaatgcgc
     7561 cgtggttatc aacgtctggc gaagtggatt gatggttttg ccggggcgtt atttgccgga
     7621 tttggcattc atttgattat ttcgcggtga tgccagacgc gtcttcagag taagtcggat
     7681 aaggcgttta cgccgcatcc gacattattt ttcacgcatg cctcgccgat gctaacagcg
     7741 ctcccaccag cataaacaac gagccgaaaa tcttattcag cgccttcatc tgctttggtc
     7801 ctttaatcca tagagcaatc ctttgagcaa gggtggcgta accgatcatc acaataatat
     7861 cgaccacaat agtggtgacg ccgagcacga tatactgcat cagttgcggc tgttgcggca
     7921 tgatgaattg cggaaatagc gccgccagaa acacaatact tttgggattg gtgagattca
     7981 caaaaactgc gcgctggaac aaatgtcgac gcgattgagt agaggccagc gatttaagat
     8041 caattgcgcc agcggcgcgc cactgctgga ttcccagcca aatcaagtaa gccgcgcctg
     8101 cccactttaa cacttcaaac gcaatcactg agcgggaaaa tagcgtcccc aaccccacgc
     8161 caaccagcac aatatgaatc gccagtccgg tctgaagccc agcaatagac gccaccgcgc
     8221 cgcgataacc gtggttgagc gaggtggtca tagtgttgat tgcaccagag cctggcgaca
     8281 ggcttaaaat gatcgatgtc agcaggtagg caaaccacca ttctaaggtc atgatgaact
     8341 cccggtgtgt ctatttttgt gccacaatac gctactgtcg cagcgttgtg tcaggcacgc
     8401 ttaaaaaaac gattttacgt ggtttaagag gcagattacc cgatgtttca gcagcaaaaa
     8461 gactgggaaa caagagaaaa cgcgtttgct gcttttacca tgggaccgct gactgatttc
     8521 tggcgtcagc gtgatgaagc agagtttact ggtgtggatg acattccggt gcgctttgtc
     8581 cgttttcgcg cacagcacca tgaccgggtg gtagtcatct gcccggggcg tattgagagc
     8641 tacgtaaaat atgcggaact ggcctacgac ctgttccatt tggggtttga tgtcttaatc
     8701 atcgaccatc gcgggcaggg acgttccggt cgcctgttag ccgatccgca tctcgggcat
     8761 gttaatcgct ttaatgatta tgttgatgat ctggcggcat tctggcagca ggaggttcag
     8821 cccggtccgt ggcgtaaacg ctatatactg gcacattcga tgggcggtgc gatctccaca
     8881 ttatttctgc aacgccatcc aggtgtatgt gacgccattg cgctaactgc gccaatgttt
     8941 gggatcgtga ttcgtatgcc gtcatttatg gcacggcaga tcctcaactg ggccgaagcg
     9001 catccacgtt tccgtgatgg ctatgcaata ggcaccgggc gctggcgcgc gttgccgttt
     9061 gctatcaacg tactgaccca cagcagacag cgatatcgac gtaacttacg cttctatgct
     9121 gatgacccaa cgattcgcgt cggtgggccg acctaccatt gggtacgcga aagtattctg
     9181 gctggcgaac aggtgttagc cggtgcgggt gatgacgcca cgcaaacgct tctcttacaa
     9241 gctgaagagg aacgcgtggt ggataaccgc atgcacgacc gtttttgtga actccgcacc
     9301 gccgcgggcc atcctgtcga aggaggacgg ccgttggtaa taaaaggtgc ttaccatgag
     9361 atcctttttg aaaaggacgc aatgcgctca gtcgcgctcc acgccatcgt tgattttttc
     9421 aacaggcata actcacccag cggaaaccgc tctacagagg tttaaatttc ttatgtacca
     9481 ggttgttgcg tctgatttag atggcacgtt actttctccc gaccatacgt tatcccctta
     9541 cgccaaagaa actctgaagc tgctcaccgc gcgcggcatc aactttgtgt ttgcgaccgg
     9601 tcgtcaccac gttgatgtgg ggcaaattcg cgataatctg gagattaagt cttacatgat
     9661 tacctccaat ggtgcgcgcg ttcacgatct ggatggcaat ctgatttttg ctcataacct
     9721 ggatcgcgac attgccagcg atctgtttgg cgtagtcaac gacaatccgg acatcattac
     9781 taacgtttat cgcgacgacg aatggtttat gaatcgccat cgcccggaag agatgcgctt
     9841 ttttaaagaa gcggtgttca aatatgcgct gtatgagccg ggattactgg agccggaagg
     9901 cgtcagcaaa gtgtttttca cctgcgattc ccatgaacaa ctgctgccgc tggagcaggc
     9961 gattaacgct cgctggggcg atcgcgtcaa cgtcagtttc tctaccttaa cctgtctgga
    10021 agtgatggcg ggcggcgtgt cgaaaggcca tgcgctggaa gcagtggcga agaaactggg
    10081 ctacagcctg aaggattgta ttgcgtttgg tgacgggatg aacgacgccg aaatgctgtc
    10141 gatggcgggg aaaggctgca ttatgggcag tgcgcaccag cgtctgaaag atctgcatcc
    10201 ggagctggaa gttattggta ctaatgccga cgacgcggtg ccgcattatc tgcgtaaact
    10261 ctatttatcg taatcgttct ttatttggtc agttgtcaac ctgatacttc gctacaatgg
    10321 atactcgtta atcaaagagt tttccattgt ggcgttactt atcatcacca cgattctgtg
    10381 ggccttctcc tttagctttt atggcgagta ccttgcgggg cacgtcgata gctattttgc
    10441 ggtgctggtg cgcgttggct tggcggcact cgtttttctg ccgtttctgc gtacccgtag
    10501 caatagcctg aaaacggtcg gcctgtatat gctggtgggc gcgatgcagc tcggcgtgat
    10561 gtatatgctg agtttccgcg cttatctcta cctgacggtc tccgaattgt tgctgttcac
    10621 cgtactgacg ccgctctaca tcacgctgat ttatgacatc atgagtaagc gccgtctgcg
    10681 ctggggctat gcttttagcg ccttgctggc ggtgattggt gccgggatta ttcgctatga
    10741 tcaggtcacc gaccatttct ggactggctt gttgctggtg caactctcca atatcacttt
    10801 tgccatcggc atggtgggtt acaaacgcct gatggaaact cgcccgatgc cgcagtataa
    10861 cgcctttgcg tggttctatc ttggcgcgtt cctggtggcg gtaattgcat ggttcttgct
    10921 gggaaatgcg cagaaaatgc cgcaaaccac gttgcaatgg ggcattctgg tgtttcttgg
    10981 cgtggtggct tccgggattg gctactttat gtggaactac ggcgcgacgc aggtggacgc
    11041 cggaacgctg ggcattatga ataatatgca cgttccggca gggctgctgg taaacctggc
    11101 tatctggcac caacagccgc actggccaac gtttattaca ggcgcgctgg tgatcctggc
    11161 ctcactgtgg gtgcatcgta agtgggtcgc tccgcgctct tcacaaacgg cagatgatcg
    11221 caggcgtgat tgcgcgctga gcgaataaac gcttccgtaa ctggctgacg ctgctcgcca
    11281 tcgcgcacgg cggcgtacag tcggctccac aagccttcgc ccagggtttt ggtcaccacc
    11341 agaccctggc gctcaaaact ctctactacc caatgcggta gcgcggcaat acccatccgc
    11401 gcggcaacca tttgaatcaa caataaggtg ttatcgacgc ttttcagtga cgggctgacg
    11461 cctgccggct gaagaaaatg ccgccagaca tccagtcgac tacgctgcac cggataaatt
    11521 aatagcgtct cgctggcgag atcttccggt gtaatacgcg tttttgccgc cagtggatgg
    11581 tcaggtgcta acaccaggcg tacttcataa tcgaacatcg gcgaataatg caggccactg
    11641 cgcggcagga tatcggaggt catcaccaga tccagctcgc cctgttgcaa cgcgggctgc
    11701 gggtcaaacg tcacgcccga tttaaaatcc atctctacct gcggccagtt cttatggaaa
    11761 ttttctaaag cgggtgtcag ccactgaata cagctatggc actcaatggc aatgcgcaga
    11821 cgcgtctgct gcggttcatt gcaggcttgc agtgcctggc tgatttgcgg cagcacctgg
    11881 tttgccagtt gcaacaggat ttctccctgc ggtgtaaagc gtagcggctg gctcttacgc
    11941 acaaatagcc ggaagccaag gcgttgttcc agatcgctaa actggtgaga cagggcggat
    12001 tgcgtctgat gcaacgttgc cgcagcggct gcgagcgagc cgcagttccg caacgcttgt
    12061 agcgttttca ggtgttttac ttcgatcatg aaagtccttc acttcggcat gaataatttg
    12121 cgcttgagga atatacagta accgccaatt atggatgtgt aaacatctgg acggctaaaa
    12181 tcattcgtct tttaaattta tggtgcgttg gctgcgtttc tccaccccgg tcacttactt
    12241 cagtaagctc ccggcgatga ataaacttgc cgccttccct aaattcaaaa tccataggat
    12301 ttacatataa ttagaggaag aaaaatgaca atattgaatc acaccctcgg tttccctcgc
    12361 gttggcctgc gtcgcgagct gaaaaaagcg caagaaagtt attgggcggg gaactccacg
    12421 cgtgaagaac tgctgacagt agggcgtgaa ctgcgtgctc gtcactggga tcaacaaaag
    12481 caagcgggta tcgacctgct gccggtgggc gattttgcct ggtacgatca tgtactgacc
    12541 accagtttgc tgctgggtaa tgttccgcca cgtcatcaga acaaagacgg ttcggtagat
    12601 atcgacaccc tgttccgtat tggtcgtgga cgtgcgccga ctggcgaacc tgcggcggca
    12661 gcggaaatga ccaaatggtt taacaccaac tatcactaca tggtgccgga gttcgttaaa
    12721 ggccaacagt tcaaactgac ctggacgcag ctgctggagg aagtggacga ggcgctggcg
    12781 ctgagccaca acgttaagcc tgtgctgctg gggccggtta cttacctgtg gctggggaaa
    12841 gtgaaaggtg aacagtttga ccgccttagc ctgctgaacg acattctgcc ggtttatcag
    12901 caagtgctgg cagaactggc gaaacgcggc atcgagtggg tacagattga tgaacccgcg
    12961 ctggtactgg aactaccaca ggcgtggctg gacgcataca aacccgctta cgacgcgctc
    13021 cagggacagg tgaaactgct gctgaccacc tattttgaag gcgtaacgcc aaatctcgac
    13081 acgattactg cgctgcctgt tcaggggctg catgtggacc ttgtacatgg taaagataac
    13141 gttgttgaac tgcacaagcg cctgccttct gactggctgc tgtctgcggg tctgatcaat
    13201 ggtcgtaacg tctggcgcgc cgatcttact gagaaatatg cgcaaattaa ggacattgtc
    13261 ggcaaacgtg atttgtgggt ggcatcttcc tgctcgttgc tgcacagccc catcgacctg
    13321 agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt ttgccttcgc cctacaaaaa
    13381 tgccatgaac tggcactgct gcgcgatgcg ctgaacagtg gtgacacggc agctttggca
    13441 gagtggagcg ccccaattca ggcgcgtcgt cactctactc gtgtacataa tccggcggta
    13501 gaaaagcgtc tggcggcgat caccgcccag gacagccagc gtgcgaatgt ctatgaagtg
    13561 cgtgctgaag cccagcgtgc gcgttttaaa ctgccagcgt ggccgaccac cacgattggt
    13621 tccttcccgc aaaccacgga aattcgtacc ctgcgtctgg atttcaaaaa gggcaatctc
    13681 gacgccaaca actaccgcac gggcattgcg gaacatatca agcaggccat tgttgagcag
    13741 gaacgtttgg gactggatgt gctggtacat ggcgaggccg agcgtaatga catggtggaa
    13801 tactttggcg agcacctcga cggatttgtc tttacgcaaa acggttgggt acagagctac
    13861 ggttcccgct gcgtgaagcc accgattgtc attggtgaca ttagccgccc ggcaccgatt
    13921 accgtggagt gggcgaagta tgcgcaatcg ctgaccgaca aaccggtgaa agggatgctg
    13981 acggggccgg tgaccatact ctgctggtcg ttcccgcgtg aagatgtcag ccgtgaaacc
    14041 atcgccaaac agattgcgct ggcgctgcgt gatgaagtgg ccgatctgga agccgctgga
    14101 attggcatca tccagattga cgaaccggcg ctgcgcgaag gtttaccgct gcgtcgtagc
    14161 gactgggatg cgtatctcca gtggggcgta gaggccttcc gtatcaacgc cgccgtggcg
    14221 aaagatgaca cacaaatcca cactcacatg tgttattgcg agttcaacga catcatggat
    14281 tcgattgcgg cgctggacgc agacgtcatc accatcgaaa cctcgcgttc cgacatggag
    14341 ttgctggagt cgtttgaaga gtttgattat ccaaatgaaa tcggtcctgg cgtctatgac
    14401 attcactcgc caaacgtacc gagcgtggaa tggattgaag ccttgctgaa gaaagcggca
    14461 aaacgcattc cggcagagcg cctgtgggtc aacccggact gtggcctgaa aacgcgcggc
    14521 tggccagaaa cccgcgcggc actggcgaac atggtgcagg cggcgcagaa tttgcgtcgg
    14581 gggtaaatcc aaaccgggtg gtaataccac ccggtctttt ctcattacag cgacttcttc
    14641 ccaccatact gcttaaacca ttccagcata cgctgccagc catctttcgc agattcggca
    14701 tgatagctcg ggcgataatc agcgttgaat gcatgcccgg catccgggta cacgataatc
    14761 tctgctttcg cattagcagc ccgcagcgcc tggcgcatcg tttcaacgct ctcctgcgga
    14821 atgctgttat cctgaccacc atataagccg agaaccggcg cgttaagatc ggttgcgata
    14881 tcaacaggtt gtttcggtga attcagcgac ttgtcgcccg tcagtttgcc gtaccacgcc
    14941 actgcggctt ttagctgtgg attatgcgcg gcatacagcc aggtgatacg tccaccccag
    15001 cagaatccgg tgatcattaa acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg
    15061 acatgatcga gatcggccag cacctgcgag tcaggcactt ttgctaccag accgctaagc
    15121 aacgtgggga tatcggcaaa atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg
    15181 atagccagat acccctccag cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg
    15241 ccaaaaattt cctgcactac aatgaccact ggcagtgggc catcgctttg ctttggtctg
    15301 gcatggtaag caggcatgtt atccccttgt gaagggatag aggtgaagcc cgccacaatc
    15361 gcgtcgtccg gggtctgaac gatggtcgaa gcgagaggcg atgcagcagg tgcaaatcca
    15421 gattgttgtg ttgttgccat ggtattctcc gtacccttat aaaaatgttg cgcaatgtta
    15481 actatagtca gcatgcaaca aatcacattg cctgaatcgg ctcatctttt atgcagtcct
    15541 gcagaatgaa gagtgattta tgtgatatga atcacttttg gtgggtaaat ttatgcaacg
    15601 catttgcgtc atggtgatga gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt
    15661 tttgcttctt ctgactaaac cgattcacag aggagttgta tatgtccaag tctgatgttt
    15721 ttcatctcgg cctcactaaa aacgatttac aaggggctac gcttgccatc gtccctggcg
    15781 acccggatcg tgtggaaaag atcgccgcgc tgatggataa gccggttaag ctggcatctc
    15841 accgcgaatt cactacctgg cgtgcagagc tggatggtaa acctgttatc gtctgctcta
    15901 ccggtatcgg cggcccgtct acctctattg ctgttgaaga gctggcacag ctgggcattc
    15961 gcaccttcct gcgtatcggt acaacgggcg ctattcagcc gcatattaat gtgggtgatg
    16021 tcctggttac cacggcgtct gtccgtctgg atggcgcgag cctgcacttc gcaccgctgg
    16081 aattcccggc tgtcgctgat ttcgaatgta cgactgcgct ggttgaagct gcgaaatcta
    16141 ttggcgcgac aactcacgtt ggcgtgacag tttcttctga taccttctac ccaggtcagg
    16201 aacgttacga tacttactct ggtcgcgtag ttcgtcactt taaaggttct atggaagagt
    16261 ggcaggcgat gggcgtaatg aactatgaaa tggaatctgc aactctgctg accatgtgtg
    16321 caagtcaggg cctgcgtgcc ggtatggtag cgggtgttat cgttaaccgc acccagcaag
    16381 agatcccgaa tgctgagacg atgaaacaaa ccgaaagcca tgcggtgaaa atcgtggtgg
    16441 aagcggcgcg tcgtctgctg taattctctt ctcctgtctg aaggccgacg cgttcggcct
    16501 tttgtatttt tgcgtagcgc ctcgcaggaa atgcctttcc aactggacgt ttgtacagca
    16561 caattctatt ttgtgcgggt aagttgttgc gtcaggaggc gttgtggatt tctcaatcat
    16621 ggtttacgca gttattgcgt tggtgggtgt ggcaattggc tggctgtttg ccagttatca
    16681 acatgcgcag caaaaagccg agcaattagc tgaacgtgaa gagatggtcg cggagttaag
    16741 cgcggcaaaa caacaaatta cccaaagcga gcactggcgt gcagagtgtg aattgctcaa
    16801 taacgaagtg cgcagcctgc aaagtattaa cacctctctg gaggccgatc tgcgtgaagt
    16861 aaccacgcgg atggaagccg cacagcaaca tgctgacgat aaaattcgcc agatgattaa
    16921 cagcgagcag cgcctcagtg agcagtttga aaacctcgcc aaccgtattt ttgagcacag
    16981 caatcgccgg gttgatgagc aaaaccgtca gagtctgaac agcctgttgt cgccgctacg
    17041 tgaacaactg gacggtttcc gccgtcaggt tcaggacagc ttcggtaaag aagcacaaga
    17101 acgccatacc ctgacccacg aaattcgcaa tctccagcaa ctcaacgcgc aaatggccca
    17161 ggaagcgatc aacctgacgc gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg
    17221 cgaggtagta ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt atgaatatga
    17281 aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg
    17341 gctgccgcag ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg cctatgaacg
    17401 ctattttaac gccgaagacg actacacccg cgaaagcgcg ctacaggaac atatcgcgtc
    17461 ggtgcgtaac catatccgtt tgctgggacg caaagattat caacagctgc cggggctgcg
    17521 aactctggat tacgtgctga tgtttattcc cgttgaaccc gcttttttac tggcgcttga
    17581 ccgccagccg gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac
    17641 tacgctgctg gtggcgttgc gtactattgc caacctgtgg cgttatgagc atcaaagccg
    17701 taacgcccag caaatcgccg atcgtgccag caagctgtac gacaagatgc gtttgttcat
    17761 cgatgacatg tccgcgattg gtcaaagtct cgacaaagcg caggataact accgccaggc
    17821 aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg
    17881 tttaggagta gaaattaaac gcgagattaa tccggatttg gctgaacagg cggtgagcca
    17941 ggatgaagag tatcgacttc ggtcggttcc ggagcagccg aatgatgaag cttatcaacg
    18001 cgatgatgaa tataatcagc agtcgcgcta gcccattagg ggtagttaag ccgggtagaa
    18061 atctagggca tcgacgccca atctgttaca cttctggaac aattttttga tgagcaggca
    18121 ttgagatggt ggataagtca caagaaacga cgcactttgg ttttcagacc gtcgcgaagg
    18181 aacaaaaagc ggatatggtc gcccacgttt tccattccgt ggcatcaaaa tacgatgtca
    18241 tgaatgattt gatgtcattt ggtattcatc gtttgtggaa gcgattcacg attgattgca
    18301 gcggcgtacg ccgtgggcag accgtgctgg atctggctgg tggcaccggc gacctgacag
    18361 cgaaattctc ccgcctggtc ggagaaactg gcaaagtggt ccttgctgat atcaatgaat
    18421 ccatgctcaa aatgggacgc gagaagctgc gtaatatcgg tgtgattggc aacgttgagt
    18481 atgttcaggc gaacgctgag gcgctgccgt tcccggataa cacctttgat tgcatcacca
    18541 tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc actgcgttca atgtatcgcg
    18601 tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc gaagccaatt atcgagccgc
    18661 tgagcaaagc ctatgatgca tactccttcc atgtgctgcc gcgtattggc tcactggtcg
    18721 cgaacgacgc cgacagctac cgttatctgg cagaatccat ccgtatgcat cccgatcagg
    18781 ataccctgaa agccatgatg caggatgccg gattcgaaag tgtcgactac tacaatctga
    18841 cggcaggggt tgtggcgctg catcgtggtt ataagttctg acaggagacc ggaaatgcct
    18901 tttaaacctt tagtgacggc aggaattgaa agtctgctca acaccttcct gtatcgctca
    18961 cccgcgctga aaacagcccg ctcgcgtctg ctgggtaaag tattgcgcgt ggaggtaaaa
    19021 ggcttttcga cgtcattgat tctggtgttc agcgaacgcc aggttgatgt actgggcgaa
    19081 tgggcaggcg atgctgactg caccgttatc gcctacgcca gtgtgttgcc gaaacttcgc
    19141 gatcgccagc agcttaccgc actgattcgc agtggtgagc tggaagtgca gggcgatatt
    19201 caggtggtgc aaaacttcgt tgcgctggca gatctggcag agttcgaccc tgcggaactg
    19261 ctggcccctt ataccggtga tatcgccgct gaaggaatca gcaaagccat gcgcggaggc
    19321 gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga agccattact
    19381 gaagagtggc gtatggcacc cggtccgctt gaagtggcct ggtttgcgga agagacggct
    19441 gccgtcgagc gtgctgttga tgccctgacc aaacggctgg aaaaactgga ggctaaatga
    19501 cgccaggtga agtacggcgc ctatatttca tcattcgcac ttttttaagc tacggacttg
    19561 atgaactgat ccccaaaatg cgtatcaccc tgccgctacg gctatggcga tactcattat
    19621 tctggatgcc aaatcggcat aaagacaaac ttttaggtga gcgactacga ctggccctgc
    19681 aagaactggg gccggtttgg atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt
    19741 ttccaccgca tattgccgat cagctggcgt tattgcagga caaagttgct ccgtttgatg
    19801 gcaagctggc gaagcagcag attgaagctg caatgggcgg cttgccggta gaagcgaggt
    19861 ttgacgattt tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt cataccgcgc
    19921 gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat attttgccgg
    19981 tcattaaagc ggatctgaaa cttatctacc gtctggctcg ctgggtgccg cgtttgctgc
    20041 cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag accttgattg
    20101 atgaactgaa tttgctgcgg gaatctgcca acgccattca gcttcggcgc aattttgaag
    20161 acagcccgat gctctacatc ccggaagttt accctgacta ttgtagtgaa gggatgatgg
    20221 tgatggagcg catttacggc attccggtgt ctgatgttgc ggcgctggag aaaaacggca
    20281 ctaacatgaa attgctggcg gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg
    20341 acagcttttt ccatgccgat atgcaccctg gcaacatctt cgtaagctat gaacacccgg
    20401 aaaacccgaa atatatcggc attgattgcg ggattgttgg ctcgctaaac aaagaagata
    20461 aacgctatct ggcagaaaac tttatcgcct tctttaatcg cgactatcgc aaagtggcag
    20521 agctacacgt cgattctggc tgggtgccac cagataccaa cgttgaagag ttcgaatttg
    20581 ccattcgtac ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt tcgtttggac
    20641 atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac
    20701 tggtgttact ccagaaaacc ctgctctatg tcgaaggggt aggacgccag ctttatccgc
    20761 agctcgattt atggaaaaca gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg
    20821 gtattcctgc cctggtgaga gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc
    20881 cagaactgcc tgaactggtt tacgacagtt tgcgccaggg caagtattta cagcacagtg
    20941 ttgataagat tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt
    21001 ttctcggaat tggcgctacg ttagtattaa gtggcacatt cttgttggtc agccgacctg
    21061 agtgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg tttgtcggtt
    21121 ggcgcaaaac acgctgattt tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc
    21181 tttgtttaat catcatctac cacagaggaa catgtatggg tggtatcagt atttggcagt
    21241 tattgattat tgccgtcatc gttgtactgc tttttggcac caaaaagctc ggctccatcg
    21301 gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa
    21361 agcaggataa aaccagtcag gatgctgatt ttactgcgaa aactatcgcc gataagcagg
    21421 cggatacgaa tcaggaacag gctaaaacag aagacgcgaa gcgccacgat aaagagcagg
    21481 tgtaatccgt gtttgatatc ggttttagcg aactgctatt ggtgttcatc atcggcctcg
    21541 tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgcg
    21601 cgttgcgttc actggcgaca acggtgcaga acgaactgac ccaggagtta aaactccagg
    21661 agtttcagga cagtctgaaa aaggttgaaa aggcgagcct cactaacctg acgcccgaac
    21721 tgaaagcgtc gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg
    21781 caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat ccataacccg gtggtgaaag
    21841 ataatgaagc tgcgcatgag ggcgtaacgc ctgccgctgc acaaacgcag gccagttcgc
    21901 cggaacagaa gccagaaacc acgccagagc cggtggtaaa acctgctgcg gacgctgaac
    21961 cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga
    22021 tactcaaccg cttatcacgc atctgattga gctgcgtaag cgtctgctga actgcattat
    22081 cgcggtgatc gtgatattcc tgtgtctggt ctatttcgcc aatgacatct atcacttggt
    22141 atccgcgcca ttgatcaagc agttgccgca aggttcaacg atgatcgcca ccgacgtggc
    22201 ctcgccgttc tttacgccga tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc
    22261 ggtgattctc tatcaggtgt gggcatttat cgccccagcg ctgtataagc atgaacgtcg
    22321 cctggtggtg ccgctgctgg tttccagctc tctgctgttt tatatcggca tggcattcgc
    22381 ctactttgtg gtctttccgc tggcatttgg cttccttgcc aataccgcgc cggaaggggt
    22441 gcaggtatcc accgacatcg ccagctattt aagcttcgtt atggcgctgt ttatggcgtt
    22501 tggtgtctcc tttgaagtgc cggtggcaat tgtgctgctg tgctggatgg ggattacctc
    22561 gtcagaagac ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat
    22621 gttgctgacg ccgccggatg tcttctcgca aacgctattg gcgatcccga tgtactgtct
    22681 gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa atcgggaaga
    22741 ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag
    22801 ggcggttgtc atatggagta caggatgttt gatatcggcg ttaatttgac cagttcgcaa
    22861 tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg acgcgggagt taatgggcta
    22921 ctcatcaccg gcactaacct gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat
    22981 tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca gcagccagtg gcaagctgcg
    23041 actgaagaag cgattattga gctggccgcg cagccagaag tggtggcgat tggtgaatgt
    23101 ggtctcgact ttaaccgcaa cttttcgacg ccggaagagc aggaacgcgc ttttgttgct
    23161 cagctacgca ttgccgcaga attaaacatg ccggtattta tgcactgtcg cgatgcccac
    23221 gagcggttta tgacattgct ggagccgtgg ctggataaac tgcctggtgc ggttcttcat
    23281 tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg cgcgtggaat ttatatcggc
    23341 attaccggtt gggtttgcga tgaacgacgc gggctggagc tgcgggaatt gttgccgttg
    23401 attccggcgg agaaattgct gatcgaaaca gatgcgccgt atctgctccc gcgcgatctc
    23461 acgccaaagc catcatcccg gcgcaacgag ccagcccatc tgccccatat tttgcaacgt
    23521 attgcgcact ggcgtggaga agatgccgca tggctggctg ccaccacgga tgccaatgtc
    23581 aaaacactgt ttgggattgc gttttagagt ttgcggaact cggtgttctt cacactgtgc
    23641 ttaatctctt tattaataag attaagcaat agcatggagc gagcctcacc atcgggttcg
    23701 gtgaaaatgg cctgaaagcc ttcgaacgcg ccttcggtaa taatcacctt atcacccgga
    23761 taaggggggg ccggatcgac aatgtctttc ggtttatata ccgatagctg atgaattacc
    23821 gccgatggga ctatcgctgg cgacgcgcca aagcgcacaa agtggctgac gccacgcgta
    23881 gcgctgatgg tcgtggtatg aatcacttct ggatcaaatt ccacaaacag gtagttaggg
    23941 aataacggtt cactgactgc ggtacgttta ccccgtacga ttttttccag ggtgatcatc
    24001 ggagcgaggc aattaaccgc ctgtctctca aggtgttcct gggcacgttg aagttgcccg
    24061 cgcttgcagt acagtaaata ccaggattgc ataatgactc ttatccgttt aatcggggcg
    24121 caaggatagc aaaagcttta cgctaagtta attatattcc ccggtttgcg ttataccgtc
    24181 agagttcacg ctaatttaac aaatttacag cattgcaaag atgaacgccg tataatgggc
    24241 gcagattaag aggctacaat ggacgccatg aaatataacg atttacgcga cttcctgacg
    24301 ctgcttgaac agcagggtga gctaaaacgt atcacgctcc cggtggatcc gcatctggaa
    24361 atcactgaaa ttgctgaccg cactctgcgt gctggtgggc ctgcgctgtt gttcgaaaac
    24421 cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg
    24481 atgggcatgg ggcaggaaga tgtttcggcg ctgcgtgaag ttggtaaatt attggcgttt
    24541 ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt ttgataaact gccgcagttt
    24601 aagcaggtgt taaacatgcc gacaaagcga ctgcgtggtg cgccctgcca acaaaaaatc
    24661 gtctctggcg atgacgtcga tctcaatcgc attcccatta tgacctgctg gccggaagat
    24721 gccgcgccgc tgattacctg ggggctgacc gtaacgcgcg gcccgcataa agagcggcag
    24781 aatctgggca tttatcgcca gcagctgatt ggtaaaaaca aactgattat gcgctggctg
    24841 tcgcatcgcg gcggcgcgct ggattatcag gagtggtgtg cggcgcatcc gggcgaacgt
    24901 ttcccggttt ctgtggcgct gggtgccgat cctgccacga ttctcggtgc agtcaccccc
    24961 gttccggata cgctttcaga gtatgcgttt gccggattgc tacgcggcac caaaaccgaa
    25021 gtagtaaagt gtatttccaa tgacctcgaa gtgccagcca gtgccgaaat cgtcctggaa
    25081 gggtatatcg atcctggtga gatggcgccg gaagggccgt atggtgacca cacaggttac
    25141 tataatgaag tcgatagttt cccggtgttt accgtgacgc atattaccca gcgtgaagat
    25201 gcgatttacc attccaccta taccgggcgt ccgccagatg agcccgcggt actgggcgtg
    25261 gcgttgaacg aagtgtttgt accgattctg caaaaacagt tcccggaaat tgtcgatttt
    25321 tacctgccgc cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa aaaacagtac
    25381 gccggacacg cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca gtttatgtac
    25441 actaaatttg tgatcgtttg cgatgatgac gtcaacgcac gcgactggaa cgatgtgatt
    25501 tgggcgatta ccacccgtat ggacccggcg cgggatactg ttctggtaga aaatacgcct
    25561 attgattatc tggattttgc ctcgcctgtc tctgggctgg gttcaaaaat ggggctggat
    25621 gccacgaata aatggccggg ggaaacccag cgtgaatggg gacgtcccat caaaaaagat
    25681 ccagatgttg tcgcgcatat tgacgccatc tgggatgaac tggctatttt taacaacggt
    25741 aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga tccgacagag aaagcgcatg
    25801 acaaccttaa gctgtaaagt gacctcggta gcagctatca cggataccgt atatcgtgtc
    25861 cgcatcgtgc cagacgcggc cttttctttt cgtgctggtc agtatttgat ggtagtgatg
    25921 gatgagcgcg acaaacgtcc gttctcaatg gcctcgacgc cggatgaaaa agggtttatc
    25981 gagctgcata ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc
    26041 ctcaaagatc atcaaatcgt ggtcgacctt ccccacggag aagcgtggct gcgcgatgat
    26101 gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt
    26161 ttgctgacag cgttggcgcg taacccaaac cgtgatatca ccatttactg gggcgggcgt
    26221 gaagagcagc atctgtatga tctctgcgag cttgaggcgc tttcgttaaa gcaccttggt
    26281 ctgcaagtgg tgccggtggt tgaacaaccg gaagcaggct ggcgtgggcg tactggtacc
    26341 gtgttaacgg cggtattgca ggatcatggt acgctggcag agcatgatat ctatattgcc
    26401 ggacgttttg agatggcgaa aattgcccgt gacctgtttt gcagtgagcg taatgcgcgg
    26461 gaagatcgcc tgtttggcga tgcgtttgca tttatctgag atataaaaaa acccgcccct
    26521 gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc tatgtcgcat
    26581 ccggcaattg gtgcacgatg cctgatgcga cgctggcgcg tcttatcagg cctacattgg
    26641 tgccggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc
    26701 tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag accaaactga
    26761 acgtctttgc gttccatcag attcagcagc gtggtgctga tacgcgcacc ggaacaaccc
    26821 agcggatgac ccagcgcgat cgcaccacca ttgaggttga tcttctcgtc aatctgctcc
    26881 attagcccga gatctttaat gcacggcagg atctgcgcgg caaaggcttc gttcatctca
    26941 aacacgccga tatcgctggc agaaagcccc gcttttttca gcgccagctt cgaggccgga
    27001 accgggccgt aacccataat cgatgggtca caaccaacga ccgccatcga acgcacacga
    27061 gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag catggcagct
    27121 gcgccatcgg aaagtgcaga agatgtgccc gccgttaccg taccgtttac tggatcaaac
    27181 gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa
    27241 ttaaactgct tcaggacgcc gtcggcatcg tgaccaccag ttgggatgat ttcattttta
    27301 aatgcggccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc
    27361 tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgccgttaa gcccatcatg
    27421 cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcgacgcc gtgactcatc
    27481 ggcacatggc ctatatgctc cacgccgcca accagacatg cctgcgcatc gccagtcatg
    27541 atcattcgtg ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg attgacggta
    27601 accgccggga cagagtgcgg cacttctgcc agcagcgccg cgttacgggc gatgttaaaa
    27661 ccctgctcca gcgtctgttg cacacaaccc cagtaaatat cgtcgagggc cgccgcttcc
    27721 agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc ttccgcacgc
    27781 acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg
    27841 acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg acgggctggc
    27901 tcaaccggag gatagtacgg ttcgttatga cgcgctttat tacgcagacc ttccggcact
    27961 tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt tgcgctaccg
    28021 agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag gccgtagacc
    28081 agcgccatat ccgcttccgc cggagtggcg ataatgccct cttccagaca gcgcaccact
    28141 tcgttgacca tcgggatcat catgcgggcg ataatctctt cttcgctgaa atcgcgcttc
    28201 ggttggctca cttctgccag caggtcttca acggcggcgt cttcttcttt cttcggctta
    28261 cctttgctgt cttctttata acgccagaaa ccgaggccgt tcttctgacc aaagcggttg
    28321 gcatcaaaca gcgcgtcgat ggcatcgcgg taatctttct gcatccgctg cgggaagcct
    28381 gctgccatga cagcctgagc gtgatgcgcg gtatcaatgc ccacaacgtc cagcagatat
    28441 gccgggccca tcggccagcc aaactgtttt tccatcactt tgtcgatctt gcggaaatcc
    28501 gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac gcggttaaca
    28561 aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct cgcccaggcg
    28621 acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc taccaacggc
    28681 attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt tttccgggcg ttccagcgcg
    28741 ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt atcctggcgt
    28801 actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc aacaaccgct
    28861 tctaccacaa tatccacgcg gtcaaatccg gcgtagtcga gcgttgggtg gatcgtggag
    28921 atcacgccag ccagtttcag accatcgatt ttgccgcgct caagctgctt gttcagcagt
    28981 ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt catgacaacc
    29041 ggcacgcctt tccacgcgga ctggtaagcg ataccgccac ccatgatgcc tgcgcccagc
    29101 acagcagcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca
    29161 tattgatcgt taaggaaaat gccgaccagt gcgcgagctt cgttggtatg cgccagcggg
    29221 acaaaacttt tgttttccag atttaaggct tcttcacgac caaaacgggc cgcagcttca
    29281 atggttttta ctgcggtgat gggggccgga taatgtttcc ccgccgtttg cgcgaccatt
    29341 cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag cggttcctgc
    29401 ttcggctgac gtttcgcttt ccagtcgagg tcgccgttaa tggcctggcg taaaatcgcc
    29461 atcgcccctt caatcagttt ttctgctttg actacgccat ccaccagacc gattttcagc
    29521 gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagtgcact gtcagcgccc
    29581 agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag tttggtttcc
    29641 ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag cacgcattcg
    29701 cagccaccac ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc
    29761 aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc ttcaggaacg
    29821 aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc tttgttcgaa
    29881 cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc ctcgccgagg
    29941 ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa taccagttcg
    30001 gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc
    30061 tcctgaatcc gcaaggtgat ctggtcgtac cagatgcatc ggagtgtgtg ttttgtgtta
    30121 aaaatatgca aataaaagat taaagaaatg ccgatctgat cacgctcggc agaaatcacg
    30181 ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa
    30241 aaatggaatc actggcctcg ctctataaaa atcatatagc taccttacaa gaacggactc
    30301 gcgatgcgct gacgcgcttc aagctggatg cattacttat tcactccggc gagctgttca
    30361 acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg
    30421 tgccggtaac tcaggtgcca aactgctggt tgctggtgga tggcgtgaac aagccgaaac
    30481 tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg acctccttct
    30541 ggactgaaga tgtggaagtg atcgcgctgc cgaaagccga tggcattggt agcctgctgc
    30601 ctgctgcgcg cggcaatatc ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg
    30661 gtattgaggc cagcaacatc aacccgaaag gggtgatcga ctacctgcat tattaccgct
    30721 ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaatg
    30781 gtcatcgtgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat atcaatattg
    30841 cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac attgtggcgc
    30901 ttaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca ccggaagaga
    30961 tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgcggct gacctgaccc
    31021 gtacctggtc ggcaaaaagc gacaacgact acgcacagct ggtgaaagac gtaaatgatg
    31081 aacaactggc gctgatcgcc accatgaaag caggcgtcag ctatgtggat taccacatcc
    31141 agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc gatatgagtg
    31201 aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt attggccatc
    31261 cgctgggcct gcaggtgcat gacgtcgccg gttttatgca ggatgatagc ggtacacacc
    31321 tcgcggcacc ggcaaaatat ccgtacctgc gctacacccg tattctccag ccgggcatgg
    31381 tgttaaccat cgaaccgggt atctacttca ttgaatcgct tctggcaccg tggcgtgaag
    31441 ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg ttcggcggca
    31501 ttcgtatcga agacaacgtg gtgatccacg aaaacaacgt ggaaaacatg acccgggatc
    31561 tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg tcgttgaaga
    31621 gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa
    31681 agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg
    31741 ggtggcgggt gcgccggatg attctcaaca gctgggtttc tctgacgacg gggagccggc
    31801 gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgtcg gggaaattac
    31861 cgctgtggta gtgcgctact acggcggcat attgcttggc accggtgggt tagtgaaagc
    31921 gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca agacgccatt
    31981 aaccgaatat actttgcaat gtgaatatag tcagttaacc ggcattgaag cgttgctggg
    32041 gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc
    32101 gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta gccgtggttc
    32161 attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac taaggaagcg
    32221 gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat cttattttca
    32281 gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc gggccgcgct
    32341 tttacccaga ccttttttgt cgccctcgcc attggctcta tgctgtggtg gccgaaccgc
    32401 aaagagaagg gcgaactgaa atcccgtgag gggtttctga tagtggtgct gttttggacc
    32461 gtgctgggta gtgtcggtgc gctccctttt atcttctcgg aaagcccgaa cctcacgatt
    32521 accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac tacgctggtg
    32581 gggctggatt cgctccctca tgccatcctc ttttatcgcc agatgctgca atggtttggc
    32641 gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt gggtgggatg
    32701 cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt
    32761 gcggaaacgg cgaaaactct gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg
    32821 gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt tgcgactatc
    32881 gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgacag tccgactatt
    32941 aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct gcacttttca
    33001 ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg catgtttatc
    33061 ggcgtacagt ttacgctggt ggttatttgt acactcgtac tgtggtttca taatgtctac
    33121 agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtatcgat ggcgacaacc
    33181 gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt actgctttta
    33241 tgttcagctt ttatcggcgg ttgcgccggg tcaacgggcg gtggcctgaa agtgatccgc
    33301 atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca tccgaacgcc
    33361 gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga agccgtttgg
    33421 ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat tatcgccacg
    33481 ggcgtggatg atttttctgc ctttgcctca gttgttgcga cattgaataa cctggggcca
    33541 gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa atggatcctg
    33601 attgccaaca tgctgtttgg tcgtctcgag gtctttacat tactggtgct ctttaccccg
    33661 actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc tcaacaaggg
    33721 acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa ctggggatcc
    33781 agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaac tatgaccgtg
    33841 tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag gaatttgtca
    33901 aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta aacctggtgg
    33961 cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag tttctgatga
    34021 actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt
    34081 atcgctggta cgaccgtttt atgctcaagc tgattatgaa gatgtcaggc ggtgaaacgg
    34141 atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc gcccgagaaa
    34201 tcgcccattt aaccgacaaa ccgacgctga aataagcata aagaataaaa aatgcgcggt
    34261 c
//
LOCUS       DAOFMX010000043        33542 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.043, whole genome shotgun sequence.
ACCESSION   DAOFMX010000043 DAOFMX010000000
VERSION     DAOFMX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.043"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="QBA20_002259"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="QBA20_002259"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDQ6292783.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="QBA20_002260"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="QBA20_002260"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDQ6292784.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="QBA20_002261"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="QBA20_002261"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDQ6292785.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="QBA20_002262"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="QBA20_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDQ6292786.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="QBA20_002263"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="QBA20_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDQ6292787.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="QBA20_002264"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="QBA20_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDQ6292788.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="QBA20_002265"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="QBA20_002265"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDQ6292789.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="QBA20_002266"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="QBA20_002266"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDQ6292790.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="QBA20_002267"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="QBA20_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDQ6292791.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="QBA20_002268"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="QBA20_002268"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDQ6292792.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="QBA20_002269"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="QBA20_002269"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDQ6292793.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="QBA20_002270"
     CDS             8212..9075
                     /locus_tag="QBA20_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDQ6292794.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="QBA20_002271"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="QBA20_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HDQ6292795.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSITQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="QBA20_002272"
     CDS             10411..11028
                     /locus_tag="QBA20_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDQ6292796.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="QBA20_002273"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="QBA20_002273"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDQ6292797.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="QBA20_002274"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="QBA20_002274"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDQ6292798.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="QBA20_002275"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="QBA20_002275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDQ6292799.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="QBA20_002276"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="QBA20_002276"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDQ6292800.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="QBA20_002277"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="QBA20_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDQ6292801.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="QBA20_002278"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="QBA20_002278"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDQ6292802.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="QBA20_002279"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="QBA20_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDQ6292803.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="QBA20_002280"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="QBA20_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDQ6292804.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="QBA20_002281"
     CDS             21859..23568
                     /locus_tag="QBA20_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDQ6292805.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="QBA20_002282"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="QBA20_002282"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDQ6292806.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="QBA20_002283"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="QBA20_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="QBA20_002284"
     tRNA            27625..27719
                     /locus_tag="QBA20_002284"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            28019..29203
                     /locus_tag="QBA20_002285"
     CDS             28019..29203
                     /locus_tag="QBA20_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ6292807.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29615..30658
                     /locus_tag="QBA20_002286"
     CDS             29615..30658
                     /locus_tag="QBA20_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDQ6292808.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31173..31601
                     /locus_tag="QBA20_002287"
                     /pseudo
     CDS             31173..31601
                     /locus_tag="QBA20_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31631..32782)
                     /locus_tag="QBA20_002288"
                     /pseudo
     CDS             complement(31631..32782)
                     /locus_tag="QBA20_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32847..33501
                     /locus_tag="QBA20_002289"
                     /pseudo
     CDS             32847..33501
                     /locus_tag="QBA20_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8398 a         8820 c         8268 g         8056 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatggttt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct ctggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcac
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtagatg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacc gatatcaaag tgaaaaccgc aaaacccaaa gacaaaccct ataagcttgc
    28081 tgatggcggc ggtatgtatc tactgattaa cactaatggt tctaaatact ggcgcatgaa
    28141 gtaccgcttt gctggtaaag aaaagatgct ttctattggc gtataccccg atgtaacgct
    28201 agccgatgct cgcgagaaac gcagtgaagc caggaaactc ctcgctgctg gaggcgatcc
    28261 aggggaggca aaaaaggaag aaaaaatcgc gcagcaaatg agtctgaaaa atacgttcga
    28321 ggctattgct cgcgaatggc accagttaaa agctgatcgg tggtcattac gctatcgtga
    28381 tgaaatcatt gatacctttg aaaaagacat ttttccctac attggcaagc gccctattgc
    28441 cgaaatcaaa ccaatgaagt tactggaagc actgcgtaaa atggaaaaac gtggagctct
    28501 ggagaaaatg cgcaaggtcc gccagcgctg tggtgaggtg ttccgctatg caattgttac
    28561 tggccgggca gactataacc cagcccccga tcttgccagt gctttagcta caccgaaaaa
    28621 agtacatttt ccctttctta ctgctaatga acttccccac ttcctcaacg atctggcggg
    28681 ttataccgga agtataatca ctaagacagc tactcagatc attatgttaa ccggcgtacg
    28741 aacacaagaa ctgcgttttg cacgctggga agatatcgat tttgagacaa aattgtggga
    28801 gatcccggca gaagtaatga agatgaagcg acctcatatt gtgccgctct ctgagcaggt
    28861 cattatgcta tttaagcagc ttgaaccaat ctcaaaacat catcccctag tctttatcgg
    28921 tagaaatgat ccccgcaaac caattagtaa ggaaagcatt aaccaagtaa ttgaattact
    28981 tggatataag ggaagactca cagggcacgg tttcagacac accatgagta caattctgca
    29041 tgaacaaggc tttaattccg cctggattga aatgcagtta gctcatgtgg ataaaaattc
    29101 catcaggggt acctataatc atgccctgta tctcgatggc cgccgtgaaa tgatgcaatg
    29161 gtacgctgat tacattgatt cactctccag ccgggaaagt taagtcagaa atacttttcc
    29221 ctgatgtttt cctcaattaa gtattttatc ctctggccta caaggctgga ggatttttgt
    29281 tattatatta tttttttgcg tcttcaaact tattacaaaa acataattaa aaacaaattg
    29341 acatttttaa attggcaatt acaaaataca gccttgcagt tatttcgata cctttagcaa
    29401 aggaatagtt gattcagata taatttagca tttcaatcat ctgacatcat tataaaaaat
    29461 gagattcaca taaagataaa attattgttc tcaaggaaag catgaagggg cttcgtaagc
    29521 aatattcatt cggtgccaag acaggacttg atacacagaa tctcaacttt tatgctctca
    29581 gactatatca ccacatgttt tacaggctca gaaaatgaac gatagattat gctttgaagt
    29641 gcacgataac aagggttatt tcgtatttcc ggatacctgg tttggcccat tgctggggga
    29701 gtttgaagaa gtgcttgatg catacgacgc tgatgaaatc agcgagacga gttacatcaa
    29761 caaactccga cgccttgctc aacgggagcc ggactttatt gatatacacg ctcatctggc
    29821 atatgcattt ctggaacaga atgccccgcg taaggcactg aacgccgctc ttaaaggact
    29881 ggcggcaggt aatcgtatca tcccggaaag tttctgtggt gaaataatct ggatgcatcc
    29941 cgaaaaccgg ccttatctgc gcgcgcttta tgcagcaatt ctcgcaaatg tgcacttaca
    30001 gcgccatcag gatgcggtta tgctcactga taaaatactg gcatacaatc ctgaagataa
    30061 tcagggggct cgctggttgt tgggttcaga gctgttacgc accggcgatc atgagcgagc
    30121 cttcagtgta ctgaaagagc atgctgatga attttcacct tactggtatg aactcggtct
    30181 cctgcatttt ctgaacgggg aacatgttaa ggccgccaca gcttttcgcc atggctttgc
    30241 cacaaacact tacatcgcag aaatgctgtg cggaaatctg catccatttc cacttgctgt
    30301 gtggcatgac ttctccggct ctctggatac ggcagaagat tactatgcaa catactcacc
    30361 attatgggga caatatccgg aagcattgct gtttgtaaac tggctctata accattcctc
    30421 cgtgctgcat gaacgatcag aaatcattaa atgcgccgaa atgctgattc aggaagatga
    30481 tttcgaaata tgcgaaagta ttttaagaca gcaggagcac ctgtggaaaa gaattgacaa
    30541 gacactttct gaggaaattg tacagaaatg cagaaatatg aacggtgaat atatctggcc
    30601 ctggatattg cccttttccg cagcagggat gaaacattca agcatccagt atcagtagat
    30661 attgcattag tgtaacttta aaacaagtaa taatttatta tccaataata acactgtgtt
    30721 ccttccccag gtgattaccc tggggttatt ttctgaaagt ttcctttgca aaaggatagc
    30781 gttgtaaatt actttaacga attactccat gaactcatta aagttctttc gtaatacttt
    30841 atcgtcaaac acgttatagc tggcatcaaa aaccacagat ttaccctgcc agcgggtaat
    30901 cccctcctct ggcgttttcg gttgaactgg tatccccgtc caacacaggc agccattata
    30961 aacagtaaaa aattgtattc gctgttatga aatcaatagt cctgacgtac tgttgaatgt
    31021 gcttgtgttc tgaacctcgt acaaccagta catttgatat cgaattataa acaaaacatt
    31081 gcatccaact tttttaatat ggacaatagt tgtccatacg gtggttttat attcgataaa
    31141 tcaatggatt tcattgagtc acaggactgg taatggatga aaatgcttta aggtttgcct
    31201 catactggcg caactcgctt gcagatgctg agtcaggaaa gggctgtttt gaacggaaag
    31261 acgcccaaaa tttcactcac tggcatggga tagcggcggg acgtcttgac gaagcgattg
    31321 tcaataaatt ttttaaggga gaaaaagacg atgtcgaaac tgtcaatgtc atcttacgcc
    31381 caaaagttta tttccggtta ctgcagcatg gtaaggaccg ttccgcaggc acgcctgata
    31441 ttgttacacc gatagtgacg ccagccttgc taagccgtga gggtttttta tatccgacgc
    31501 cagcgacctc cattcccaga gacctgcttg aacctttgcc aaaaggagca ttttcgattg
    31561 gtgagattgg gcagtatgac aaatacaaga cgacccatac tgatcctacc cacgtaatgt
    31621 ggacacggcc ctaagcgaga ttatggtttt caaattgttc cgggctgaga ccgccacagg
    31681 cactgtgacg acgccagcga ttgtaatcac actcgatata attaaacacc gttgcccgca
    31741 ttatttcccg gctgctaaag cgttccccgt ggatacattc caccttcagc gaatgaaaga
    31801 agctttccac acaggcattg tcataacagt agcctttcgc actcatactg ccacgcaggt
    31861 tgtgtcgctt cagcattgcc tgataatccc ctgaacagta ttgaccacca cggtccgtat
    31921 gaacaatgac gctttccggg cgtcttctcc gccacaacgc catttgcagt gcatcacagg
    31981 ccagttgtgc tgtcattcgc ggtgacatcg accagccaat gacggcgcgt gaccacaggt
    32041 cgatgactac tgcgagatac aaccagccct catcggtacg caagtacgtg atgtcacccg
    32101 cccacttctg gtttcgggcc actggcgctg aagtcctgct ccagcagatt ctccaatacg
    32161 ggcaggccat gtgcacggta gctgaccggg ctgaacttcc ggctggcttt cgcccgcagc
    32221 ccctgacgac gcaggctggc ggcaatggtt ttaatattga actccggcag ttcgtcagca
    32281 aggcggggag caccgtatcg ctgctttgcc tcaaagaatg ccttatggac agcggcatcg
    32341 caggtgagcc gaaactgttg gcgcaggctc atctggtgac gacgcctgag ccagacatac
    32401 cagccgctgc gggcaacccg aagtacacga cacatcgctt tgatgctgaa ctctgcccga
    32461 tgattttcga taaagacata cttcatttca ggcgcttcgc gaagtatgtc gcggcctttt
    32521 ggaggatgga cagttcctca gcctgctccg ccagttgtcg tttaaggcgg acattttcag
    32581 cggccagttc gctttcgcgc tctgacgaac tcatttgttg ctgctgttta ctgcgccagg
    32641 cataaagctg agattcatac aggctgagtt cacgggctgc ggcggccaca ccgatgcgtt
    32701 cagcgagttt cagggcttcg ttacgaaatt caggcgtatg ttgtttacgg ggcttcttgc
    32761 tgattgatac tggttttgtc atgagtcacc tctggttgag agtttactca cttagtcctg
    32821 tgtccactat tggtgggtaa gatcaccctc cattaaaact aaaacgggcg ctttctgata
    32881 tatcatctgt aagcgcagta ataccattag tagcacctct cggcggcgca ttgattattg
    32941 aatattataa ctggaatgtt atatttcttg ttatgatgct gttcagtatt ataataatgt
    33001 tattatcacc aaagataact catgcaaatg agaaagtaat atgtcaaaac agtgcatcct
    33061 ttgtgaccaa agagtttatc tgcggcacta tacttgtctc attaatactc agttctctat
    33121 tttgctacat atcaatatca ccatcaattt tcatgcatac ttatggcctg agtactttta
    33181 gttactcaat tattttctcc ttctcagttt tatttttcat aatcggaaat caattatcaa
    33241 agattgaaaa attatccaac ctatggtttt tattacccaa tagccaattt tatcgcatta
    33301 caaataaatg gttcaaaaac aggatttgcc gcatctatta caggttttac tcaaacaata
    33361 agcgctggta caatcagttt cctgtcagta accgtaagcg acaacggtgt tggtcccgga
    33421 actactttag gtcttggttc actaataact tcattactgt caatagccgt aatcgccatt
    33481 gcaggaaata ataaaaagta agtgtactct gactgcgtat gtatctactt gaaccgcccc
    33541 gg
//
LOCUS       DAOFMX010000044        33130 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.044, whole genome shotgun sequence.
ACCESSION   DAOFMX010000044 DAOFMX010000000
VERSION     DAOFMX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.044"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(2..94)
                     /locus_tag="QBA20_002290"
     tRNA            complement(2..94)
                     /locus_tag="QBA20_002290"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="QBA20_002291"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="QBA20_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDQ6292809.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="QBA20_002292"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="QBA20_002292"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDQ6292810.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="QBA20_002293"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="QBA20_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDQ6292811.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="QBA20_002294"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="QBA20_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDQ6292812.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="QBA20_002295"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="QBA20_002295"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDQ6292813.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="QBA20_002296"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="QBA20_002296"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDQ6292814.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="QBA20_002297"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="QBA20_002297"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="QBA20_002298"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="QBA20_002298"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDQ6292815.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="QBA20_002299"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="QBA20_002299"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDQ6292816.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="QBA20_002300"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="QBA20_002300"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDQ6292817.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="QBA20_002301"
                     /pseudo
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="QBA20_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="QBA20_002302"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="QBA20_002302"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDQ6292818.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="QBA20_002303"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="QBA20_002303"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDQ6292819.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="QBA20_002304"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="QBA20_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDQ6292820.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="QBA20_002305"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="QBA20_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDQ6292821.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="QBA20_002306"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="QBA20_002306"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDQ6292822.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQSQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="QBA20_002307"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="QBA20_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDQ6292823.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="QBA20_002308"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="QBA20_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDQ6292824.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="QBA20_002309"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="QBA20_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDQ6292825.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="QBA20_002310"
     CDS             complement(20900..21019)
                     /locus_tag="QBA20_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HDQ6292826.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="QBA20_002311"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="QBA20_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDQ6292827.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="QBA20_002312"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="QBA20_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDQ6292828.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25481)
                     /gene="hycC"
                     /locus_tag="QBA20_002313"
     CDS             complement(23682..25481)
                     /gene="hycC"
                     /locus_tag="QBA20_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDQ6292829.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGA
                     GSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKL
                     SNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMS
                     VSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFII
                     MAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSL
                     VPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25518..26129)
                     /gene="hycB"
                     /locus_tag="QBA20_002314"
     CDS             complement(25518..26129)
                     /gene="hycB"
                     /locus_tag="QBA20_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDQ6292830.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26254..26715)
                     /gene="hycA"
                     /locus_tag="QBA20_002315"
     CDS             complement(26254..26715)
                     /gene="hycA"
                     /locus_tag="QBA20_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDQ6292831.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26927..27277
                     /gene="hypA"
                     /locus_tag="QBA20_002316"
     CDS             26927..27277
                     /gene="hypA"
                     /locus_tag="QBA20_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDQ6292832.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27281..28153
                     /gene="hypB"
                     /locus_tag="QBA20_002317"
     CDS             27281..28153
                     /gene="hypB"
                     /locus_tag="QBA20_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDQ6292833.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28144..28416
                     /gene="hypC"
                     /locus_tag="QBA20_002318"
     CDS             28144..28416
                     /gene="hypC"
                     /locus_tag="QBA20_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDQ6292834.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28416..29537
                     /gene="hypD"
                     /locus_tag="QBA20_002319"
     CDS             28416..29537
                     /gene="hypD"
                     /locus_tag="QBA20_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDQ6292835.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29534..30544
                     /gene="hypE"
                     /locus_tag="QBA20_002320"
     CDS             29534..30544
                     /gene="hypE"
                     /locus_tag="QBA20_002320"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDQ6292836.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30618..32696
                     /gene="flhA"
                     /locus_tag="QBA20_002321"
     CDS             30618..32696
                     /gene="flhA"
                     /locus_tag="QBA20_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDQ6292837.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDTPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32733..33086)
                     /locus_tag="QBA20_002322"
     CDS             complement(32733..33086)
                     /locus_tag="QBA20_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDQ6292838.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7806 a         9233 c         8623 g         7468 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgccta gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtggg ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagatggttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgagcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactgg catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtc gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacattcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttataccca gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 cggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcagg ataatgccga tattttccag ggtgtggtaa
    24661 gccagcaggc gctggatgtt gtgttccatc aacgcataca gaccaccgac gaacgcggtg
    24721 atcatgccga gcaccagcag cgcgatcccc caccacagcg gtgcattacc gcccagcagt
    24781 gacagggtta acatgcccag caggccaatt ttcatgacta ccgtagaaaa caacgcggca
    24841 gctggcgcag aggcgttagc atgtgcctgc ggcacccagc cgtgcagcgg aataatccca
    24901 gccagcaggc caaagccaat cacaccgagc agccagatat cggaaccgag cggcagctgt
    24961 tgcatacgca tatccagcag gcgcagatcc agcgtgccat aacgctgcca cagcagccag
    25021 caagcaatca ccagcagcag agtgccaaga cgccccagcg caaaccacag tttgccctct
    25081 ttgctgttgc tggtgaggaa caccgcacac aaggccatga cttcggccat taccacgaac
    25141 atgccgaggt tgctggcgat gacggcgcag acggcggcag ccatcaacat attgatctgc
    25201 aagccgttgc acttcacctg cgcgtggcga tgccagtcaa tgttatagag gctgacaaac
    25261 agaccgcaaa ggccgagggt aatcagccaa atcgcgttaa gcggggagat ttgcacatcg
    25321 tagcttacca gcgacagcgc accgctcacg ccaaccgcgc cagtcagtac agtgaagccc
    25381 gcggctgccg tatacagact accaaccgcg ccgccaattc cggctatcca gccacttagc
    25441 gctttttgaa aagagaaatg ccagaacagc ggcggcgaca aaccatgcca cgccgctatt
    25501 gatcagggaa attgcgctca tttagcctct ccactttgag cctgctgaaa caaggtgaga
    25561 tcgccaaagt ccgtgttaaa ggtcagctca cgcttacgtt tgctgacgcg ggcgatatcg
    25621 gtgttatcca ccagatgcag ggctttagtc gggcacatcc gcacacaggc cggaccttgt
    25681 tcatcaaagc tacaaaggtc acatttgacg gcgatcgcgc gaatacctgg cacccagtca
    25741 agcaatgtgc tgacacgcgc cggagcaggc ggtgccggtg gcgctttcgg ggtattggcg
    25801 tttgccggaa tatccagcgg acggctgccg gaaaattcaa ttgcgccaaa cgggcaggcg
    25861 atgccgcaca gtttgcagct tacgcacaga ctttcattca actgcacggc cccatcaacg
    25921 cgggtgatgg cgttaaccgg gcagaccacc gcgcagggtg catcttcaca gtggtgacag
    25981 agctgcggcg cagattcttt ttcattgagc atcactctca ggcgcggcat tgattgcagg
    26041 ccgtgctggc gatgcgtctc tgaacaggcg gcctcacagg tgtggcagcc gatacagagc
    26101 gtggagtcgg caattacaaa acgattcacc aggcattcct caggtgattg tcatttttga
    26161 cgaaaacatg ccgatgaaat gtcattttcg acactcatcg acacgcccat ccccaaacag
    26221 gcgtaacgcc tgcaaaacgg gcaaagcctc agctcatgct gccgggcttt gtccctttac
    26281 cagttggctt aaattcaccg gcacattgtt ttcaatggcg gtgtataagc tgtcgtaaac
    26341 gccagcgatt ttttcgagat agtgttccag aacctgttcg cgatcgcgga tgttgacctt
    26401 gccatcaatc atgccgtcaa tgctcagttg cgcctgcgca atccgctgtt tgtcttcgcc
    26461 atcttttgtt tcgacgtaat actcgatggt gtggctgtta aagccatccg ccagggtgac
    26521 gtaaatacga aaatgttcaa aaaggacgaa acagagttct ttgtccggcg cgcagctcat
    26581 ggcgtgatgc aggcgcgctt tcgataacgt gatcccctga accagcgaat tgcagtagat
    26641 gtgccactgg tcctgtaggc gacgatgccg ctgtgcgatg taatcggctt tctcgcttat
    26701 ttcccaaata gtcattgtca ggttactcgt ttaacagaga tgccagcttt aagcattttt
    26761 tgtgccaact tttaatttat tgttattaaa gagattttta agctaaagat gaatttcgtc
    26821 gccgtgtcga cgtgtcattt cgacatcatc gacattattc accgcaggga taatcaacac
    26881 tggcacaatt attgcttgta gctggcaata gttaatggga ggcgatatgc acgaaataac
    26941 cctctgccaa cgggcactgg aattgatcga acagcaggcc gcaaaacacg gcgcaaaacg
    27001 cgtaactggg gtctggctca aaattggcgc attttcttgt gtcgaaacca gctctcttgc
    27061 cttttgtttt gatctggttt gccgcggcag cgtggcggaa ggttgtaaac tgcacctcga
    27121 agaacaagag gccgaatgct ggtgtgaaac atgccaacag tatgtgacgc tactgaccca
    27181 gcgcgtccgc cgctgtccac agtgtcatgg tgacatgcta cagattgtgg cagacgacgg
    27241 tttacagatt cggcggatag aaatagacca ggagtgagcg atgtgtacaa catgcggttg
    27301 cggtgaaggc aacctgtata tcgagggtga tgaacataac cctcattccg cgtttcgtag
    27361 cgcgccattt gccccggcgg cacgcccgca gatgaaaatc accggcatta aagcgcctga
    27421 atttaccccc agccagactg aagaaggcga cctgcattac ggtcatggcg aagcgggcac
    27481 tcacacaccg ggcatgagcc agcgtcggat gctggaagtc gaaattgacg tgctggacaa
    27541 aaacaaccgt ctggctgaac gcaaccgcgc gcgctttgct gcccgcaagc aactggtgct
    27601 caacctggtt tccagccctg gttccggtaa aaccaccctg ctgacggaaa ccttaatgcg
    27661 cctgaaagac agcgttccgt gcgcggttat tgaaggcgac cagcaaaccg tgaacgatgc
    27721 cgcacgcatt cgcgctaccg gtacaccagc gattcaggtg aacaccggta aaggctgcca
    27781 tcttgacgca cagatgattg ccgacgccgc accgcgtctg ccactggacg ataacggtat
    27841 tctgtttatc gaaaacgttg gcaacctcgt atgcccggcc agcttcgatc tcggtgaaaa
    27901 acacaaagta gcggtgcttt ccgttaccga aggtgaagac aaaccgctga aatatccgca
    27961 tatgtttgcc gccgcctcgc tgatgctgct caacaaagtt gacctgttgc cgtatctcaa
    28021 ctttgacgtt gagaagtgca tcgcctgcgc ccgcgaagtc aatccagaaa ttgaaatcat
    28081 ccttatttcc gccaccagcg gcgaagggat ggaccagtgg ctgaactggc tggagacaca
    28141 gcgatgtgca taggcgttcc cggccagatc cgcaccattg acggcaacca ggcgaaagtc
    28201 gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag tcggcagctg cgatgaaaac
    28261 ggtcagccgc gcgtgggcca gtgggtactg gttcacgttg gctttgccat gagcgtaatt
    28321 aatgaagccg aagcacgcga cactctcgac gccttacaaa acatgtttga cgttgagccg
    28381 gatgtcggcg cgctgttgta tggcgaggaa aaataatgcg ttttgttgat gaatatcgcg
    28441 cgccggaaca ggtgatgcag ttaattgagc atctgcgcga acgtgcttca catctctctt
    28501 acaccgccga acgccctctg cggattatgg aagtgtgtgg cggtcatacc cacgccattt
    28561 ttaaattcgg cctcgaccag ttactgcctg aaaacgttga gtttatccac ggtccgggtt
    28621 gcccggtgtg cgtactgccg atgggtagaa tcgacacctg cgtggagatt gccagccatc
    28681 cggaagtcat cttctgtacc tttggcgacg ccatgcgcgt accagggaaa cagggatcgc
    28741 tgttgcaggc aaaagcacgc ggtgccgatg tgcgcatcgt ttactcgccg atggatgcgt
    28801 tgaaactggc gcaggagaat ccaacccgca aagtggtgtt cttcggctta ggttttgaaa
    28861 ccaccatgcc gaccaccgcc atcactctgc aacaggcgaa agcgcgtgat gtgcagaatt
    28921 tttacttctt ctgccagcac attacgctta tcccgacgct gcgcagtttg ctggaacagc
    28981 cggataacgg tatcgacgcg ttcctcgcgc cgggccacgt tagtatggtt atcggcactg
    29041 atgcctataa ttttatcgcc agcgattttc atcgtccgct ggtggtcgct ggtttcgaac
    29101 cccttgatct actgcaaggc gtggtcatgc tggtggagca gaaaatagcg gcccacagca
    29161 aggtagagaa tcagtatcgt cgggtggtac cggatgccgg taacctgctg gcgcaacagg
    29221 cgattgccga tgtgttctgt gtcaacggcg acagcgaatg gcgcggctta ggcgtgattg
    29281 aatcttctgg cgtgcacctg acgccggatt atcaacgatt cgatgccgaa gcacatttcc
    29341 gcccggcacc gcagcaggtc tgcgatgacc cgcgcgcgcg ttgtggtgag gtattaacgg
    29401 gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac ctgtaatcct caaaccgcat
    29461 ttggtgcact gatggtctcc tccgaaggag cgtgcgccgc gtggtatcag tatcgtcagc
    29521 aggagagtga agcgtgaata atatccaact cgcccacggc agcggcggcc aggcgatgca
    29581 gcaattaatc aacagcctgt ttatggaagc ctttgccaac ccgtggctgg cagagcagga
    29641 agatcaggca cgtcttgatc tggcgcagct ggtagcggaa ggcgaccgtc tggcgttctc
    29701 caccgacagt tacgtcattg acccgctgtt ctttcctggc ggtaatatcg gcaagctggc
    29761 gatttgcggc accgcgaatg acgttgcggg cagtggcgct attccgcgct atctctcctg
    29821 tggctttatc ctcgaagaag gattgccgat ggagacactg aaagccgtag tgaccagcat
    29881 ggcagaaacc gcccgcacgg caggcattgc catcgttact ggcgatacta aagtggtgca
    29941 gcgcggcgcg gcagataaac tgtttatcaa caccgctggc atgggcgcaa ttccggcgaa
    30001 tattcactgg ggcgcacaga cgctaaccgc aggcgatgta ttgctggtta gcggtacact
    30061 cggcgaccac ggggcgacta tccttaacct gcgtgagcag ctggggctgg atggcgaact
    30121 ggtcagcgac tgcgcggtgc tgacgccgct tattcagacg ctgcgtgaca ttcccggcgt
    30181 gaaagcgctg cgtgatgcca cccgtggtgg tgtaaacgcg gtggttcatg agttcgcggc
    30241 agcctgcggt tgtggtattg aaatttctga atcagcgctg ccggttaaac ctgccgtgcg
    30301 cggcgtttgc gaattgctgg gactggacgc cctgaacttt gccaacgaag gcaaactggt
    30361 gatcgccgtt gaacgcaacg cggcagagca agtgctggca gcgttacatt cccatccact
    30421 ggggaaagac gcggcgctga ttggtgaagt ggtggaacgt aaaggtgttc gtcttgccgg
    30481 tctgtatggc gtgaaacgaa ccctcgattt accacacgcc gaaccgcttc cgcgtatatg
    30541 ctaataaaat tctaaatctc ctatagttag tcaatgacct tttgcaccgc tttgcggtgc
    30601 tttcctagaa gaacaaaatg tcatatacac cgatgagtga tctcggacag caagggttgt
    30661 tcgacatcac tcggacacta ttgcagcagc ccgatctggc ctcgctgtgt gaggctcttt
    30721 cgcaactggt aaagcgttct gcgctcgccg acaacgcggc tattgtgttg tggcaagcgc
    30781 agactcaacg tgcgtcttat tacgcgtcgc gtgaaaaaga cacccccatt aaatatgaag
    30841 acgaaactgt tctggcacac ggtccggtac gcagcatttt gtcgcgccct gatacgctgc
    30901 attgcagtta cgaagaattt tgtgaaacct ggccgcagct ggccgcaggt gggctatacc
    30961 ccaaatttgg tcactattgc ctgatgccac tggcggcggg agggcatatt tttggtggct
    31021 gtgaatttat tcgttatgac gagcgtccct ggagcgaaaa agagttcaat cgtctgcaaa
    31081 catttacgca gatcgtttct gtcgtcaccg aacaaatcca gagccgcgtc gtcaacaatg
    31141 tcgactatga gttgttatgc cgggagcgcg ataacttccg catcctggtt gccattacca
    31201 acgcggtgct ttcccgcctg gatatggacg aactggtcag cgaagtcgcc aaagaaatcc
    31261 attactattt cgacattgac gatatcagca ttgtcttacg cagccaccgt aaaaacaaac
    31321 tcaacatcta ctccactcac tatcttgata aacaacatcc cgcccacgaa cagagcgaag
    31381 tcgatgaagc cggaaccctc accgaacgcg tgttcaaaag taaagagatg ctgctgatca
    31441 atctctacga gcgggacgat ttagccccct atgaacgcat gttgttcgac acctggggca
    31501 accagattca aaccttgtgc ctgttaccgc tgatgtctgg cgacaccatg ctgggcgtgc
    31561 tgaaactggc gcaatgcgaa gagaaagtgt ttaccactac caatctgaat ttgctgcgcc
    31621 agattgccga acgtgtggca atcgctgtcg ataacgccct cgcctatcag gaaatccatc
    31681 gtctgaaaga acggctggtt gatgaaaacc tcgccctgac cgagcagctc aacaatgttg
    31741 atagtgaatt tggcgagatt attggccgca gcgaagccat gtacagcgtg cttaaacaag
    31801 ttgaaatggt ggcgcaaagt gacagtaccg tgctgatcct cggtgaaact ggctcgggta
    31861 aagagctgat tgcccgtgcg atccataatc tcagtgggcg taataatcgc cgcatggtca
    31921 aaatgaactg cgcggcgatg cctgccggat tgctggaaag cgatctgttt ggtcatgagc
    31981 gtggggcttt taccggtgcc agcgcccagc gcattggtcg ttttgaactg gcggataaaa
    32041 gctctctgtt cctcgacgaa gtgggcgata tgccactgga gttacagccg aagttgctgc
    32101 gtgtattgca ggaacaggag tttgaacgtc tcggcagcaa caaaatcatt cagacggacg
    32161 tacgtttaat tgccgcgact aaccgcgatc tgaaaaaaat ggtcgccgac cgagagttcc
    32221 gtagcgatct ctattaccgc ctgaacgtat tcccgattca cctgccgcca ctacgcgagc
    32281 gtccggaaga tactccgctg ctggcgaaag cctttacctt caaaattgcc cgtcgtctgg
    32341 ggcgcaatat cgacagcatt cctgccgaga cgctgcgcac cttgagcaac atggagtggc
    32401 cgggtaacgt acgcgaactg gaaaacgtca ttgagcgcgc agtattgcta acacgcggca
    32461 acgtgctgca gctgtcattg ccagatattg ctttaccgga gcctgaaacg ccgcctgccg
    32521 caacggttgt cgcccaggag ggcgaagatg aatatcagtt gattgtgcgc gtgctgaaag
    32581 aaaccaacag cgtggttgcc gggcctaaag gcgctgcgca acgtctgggg ctgaaacgca
    32641 cgaccctgct gtcacggatg aagcggctgg gaattgataa atcggcattg atttaactgc
    32701 aaattgccgg acagatctgc ctgtccggca tactattcac gaggtttttt cggacgatat
    32761 ttttccggca gttctggcac cggacgcttg tcatcgatga gatgacgcac ggttaagatc
    32821 ggatgacgcc acagcattct cggcccggcc caacgcataa tctgtttcat ctcttcacgc
    32881 tttgcaggct ggtaacagtg caccggacac tgcttacagg ctggtttctc ttcgccgaac
    32941 acacatttat ccagccgctt ttgcgcgtaa acaaacaacg cctcgtaatg ctccggctcc
    33001 gctgacgcct gcgggcattt cgcttgataa agatcgatca tttttttaat cgtcagtttt
    33061 tcacgagaga tacgcttgcc ggacatgctg cctccacctc attaagatgc atttatatta
    33121 catcttaatc
//
LOCUS       DAOFMX010000045        32144 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.045, whole genome shotgun sequence.
ACCESSION   DAOFMX010000045 DAOFMX010000000
VERSION     DAOFMX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.045"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="QBA20_002323"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="QBA20_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /locus_tag="QBA20_002324"
     CDS             1128..1235
                     /locus_tag="QBA20_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292839.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="QBA20_002325"
     CDS             complement(1288..2046)
                     /locus_tag="QBA20_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDQ6292840.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="QBA20_002326"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="QBA20_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDQ6292841.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="QBA20_002327"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="QBA20_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDQ6292842.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="QBA20_002328"
     CDS             3759..4619
                     /locus_tag="QBA20_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDQ6292843.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="QBA20_002329"
     CDS             complement(4660..5196)
                     /locus_tag="QBA20_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDQ6292844.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="QBA20_002330"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="QBA20_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDQ6292845.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="QBA20_002331"
     CDS             6174..6764
                     /locus_tag="QBA20_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDQ6292846.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILIPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..8287
                     /gene="tcyP"
                     /locus_tag="QBA20_002332"
                     /pseudo
     CDS             6897..8287
                     /gene="tcyP"
                     /locus_tag="QBA20_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8291..9107)
                     /locus_tag="QBA20_002333"
                     /pseudo
     CDS             complement(8291..9107)
                     /locus_tag="QBA20_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9384..9647)
                     /gene="cedA"
                     /locus_tag="QBA20_002334"
     CDS             complement(9384..9647)
                     /gene="cedA"
                     /locus_tag="QBA20_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDQ6292847.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9830..12091
                     /gene="katE"
                     /locus_tag="QBA20_002335"
     CDS             9830..12091
                     /gene="katE"
                     /locus_tag="QBA20_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDQ6292848.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12138..12896)
                     /gene="chbG"
                     /locus_tag="QBA20_002336"
     CDS             complement(12138..12896)
                     /gene="chbG"
                     /locus_tag="QBA20_002336"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDQ6292849.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12909..14261)
                     /gene="celF"
                     /locus_tag="QBA20_002337"
                     /gene_synonym="chbF"
     CDS             complement(12909..14261)
                     /gene="celF"
                     /locus_tag="QBA20_002337"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDQ6292850.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKKPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14366..15208)
                     /gene="chbR"
                     /locus_tag="QBA20_002338"
                     /pseudo
     CDS             complement(14366..15208)
                     /gene="chbR"
                     /locus_tag="QBA20_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15216..15566)
                     /gene="chbA"
                     /locus_tag="QBA20_002339"
     CDS             complement(15216..15566)
                     /gene="chbA"
                     /locus_tag="QBA20_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDQ6292851.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15625..16974)
                     /gene="chbC"
                     /locus_tag="QBA20_002340"
     CDS             complement(15625..16974)
                     /gene="chbC"
                     /locus_tag="QBA20_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDQ6292852.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(17059..17379)
                     /gene="chbB"
                     /locus_tag="QBA20_002341"
     CDS             complement(17059..17379)
                     /gene="chbB"
                     /locus_tag="QBA20_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDQ6292853.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17678..18016)
                     /gene="osmE"
                     /locus_tag="QBA20_002342"
     CDS             complement(17678..18016)
                     /gene="osmE"
                     /locus_tag="QBA20_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDQ6292854.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18218..19045
                     /gene="nadE"
                     /locus_tag="QBA20_002343"
     CDS             18218..19045
                     /gene="nadE"
                     /locus_tag="QBA20_002343"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDQ6292855.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19275..20162
                     /gene="cho"
                     /locus_tag="QBA20_002344"
     CDS             19275..20162
                     /gene="cho"
                     /locus_tag="QBA20_002344"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDQ6292856.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20122..20697)
                     /gene="ves"
                     /locus_tag="QBA20_002345"
     CDS             complement(20122..20697)
                     /gene="ves"
                     /locus_tag="QBA20_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDQ6292857.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20900..21385)
                     /gene="spy"
                     /locus_tag="QBA20_002346"
     CDS             complement(20900..21385)
                     /gene="spy"
                     /locus_tag="QBA20_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDQ6292858.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21715..22683)
                     /gene="astE"
                     /locus_tag="QBA20_002347"
     CDS             complement(21715..22683)
                     /gene="astE"
                     /locus_tag="QBA20_002347"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDQ6292859.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDILWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22676..24009)
                     /gene="astB"
                     /locus_tag="QBA20_002348"
                     /pseudo
     CDS             complement(22676..24009)
                     /gene="astB"
                     /locus_tag="QBA20_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(24006..25484)
                     /gene="astD"
                     /locus_tag="QBA20_002349"
     CDS             complement(24006..25484)
                     /gene="astD"
                     /locus_tag="QBA20_002349"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDQ6292860.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25481..26515)
                     /gene="astA"
                     /locus_tag="QBA20_002350"
     CDS             complement(25481..26515)
                     /gene="astA"
                     /locus_tag="QBA20_002350"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDQ6292861.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26512..27732)
                     /gene="astC"
                     /locus_tag="QBA20_002351"
                     /pseudo
     CDS             complement(26512..27732)
                     /gene="astC"
                     /locus_tag="QBA20_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28178..28984
                     /gene="xthA"
                     /locus_tag="QBA20_002352"
     CDS             28178..28984
                     /gene="xthA"
                     /locus_tag="QBA20_002352"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDQ6292862.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29151..29860
                     /locus_tag="QBA20_002353"
                     /pseudo
     CDS             29151..29860
                     /locus_tag="QBA20_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29865..30542
                     /gene="ydjY"
                     /locus_tag="QBA20_002354"
     CDS             29865..30542
                     /gene="ydjY"
                     /locus_tag="QBA20_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDQ6292863.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30557..31264
                     /locus_tag="QBA20_002355"
     CDS             30557..31264
                     /locus_tag="QBA20_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDQ6292864.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31264..31812
                     /locus_tag="QBA20_002356"
     CDS             31264..31812
                     /locus_tag="QBA20_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDQ6292865.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            31822..>32144
                     /locus_tag="QBA20_002357"
     CDS             31822..>32144
                     /locus_tag="QBA20_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDQ6292866.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
BASE COUNT         8243 a         8241 c         7615 g         8045 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttccagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattaacca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccgttatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg attccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7801 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7861 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7921 gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg cgcctacggt
     7981 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     8041 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     8101 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     8161 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     8221 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     8281 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     8341 aatgttccat cgcggcgatg catactgact catatttaaa cagatcgtca gcgaaaaaaa
     8401 tgtccaactt atcaatacat tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa
     8461 cctcataccc gagacgtttt ctaagtgaac tatctggaat actcataata tcttcaagca
     8521 caccacttcc cattcccgga agatttagtg gattaaaaag aagattgata ttaagcattg
     8581 atattatcga aggaaacttt aggagactct catttttttt gaatcgcagc aaaaatgctc
     8641 ccggcaggat gtatataatt tttttcatag tatatagtcc ttaaataatg aataaccaac
     8701 ctatttttta tattgatcaa attcgtcatt ttttcgggtc ggaattcaca ggtaaaatcc
     8761 atcccaacaa aattctcatt aatatctctt atccgaaact cactgccata aagattatta
     8821 attatcactc tatatttaac attgtcctgc agatacgtat caaaactaag tgctggcgct
     8881 cctagctttg aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc
     8941 gactctgcta tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc
     9001 atggtagact caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca
     9061 ccataattac tctgtattaa gtttgtagaa aacatctccc gcctcatatt gttaacaaaa
     9121 ttattatctt atttaaatct aagtcattta caatataagt ttaagagcga cgccacagga
     9181 tgaactatca aaaataactc atcatgatta gcaaaactta accattttaa aataaataaa
     9241 taattaaaga aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt
     9301 acaggtggtt tgaatgaatg tagcaacgaa atgcagaatt tcaggtcctg taactcccgg
     9361 caaaaccggg aggtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt
     9421 gcccaacgtt gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc
     9481 atcagtacaa acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc
     9541 tttatcgcat gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg
     9601 ttttgctggc ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga
     9661 aaaactgtag tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca
     9721 ccgtttccag aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt
     9781 ttgaccaaaa cagcggccct ttcagtaata aattaaggag acgagttcaa tgtcgcaaca
     9841 taacgaaaag aacccacatc agcaccagtc accactacac gattccagcg aagcgaaacc
     9901 ggggatggac tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc
     9961 gccaggtgca caacctaccg cccctgggag cctgaaagcc cctgatacac gtaacgaaaa
    10021 acttaattct ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca
    10081 gggcgtgcgc atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct
    10141 gctggaagat tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga
    10201 acgtattgtt catgcacgcg gctcagcagc tcacggttat ttccagccat ataaaagctt
    10261 aagcgatatc accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt
    10321 taggttctct accgttcagg gtggcgctgg ctctgccgat accgtgcgtg atatccgtgg
    10381 ttttgccacc aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc
    10441 aatcttcttt atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga
    10501 accgcactgg gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc
    10561 tctgcaacct gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg
    10621 cagttaccgc accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg
    10681 gaaggcaacg tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg
    10741 ggatgaagca caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga
    10801 agccattgaa gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga
    10861 agatgaattc aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact
    10921 ggtgcccgtt cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc
    10981 cgaaaacgaa caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa
    11041 cgatcccctg ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg
    11101 tgggccgaat ttccatgaga ttccgattaa ccgcccgacc tgcccttacc ataatttcca
    11161 gcgtgacggc atgcatcgta tggggatcga cactaacccg gcgaattatg aaccgaactc
    11221 gatcaacgat aactggccgc gcgaaacacc accggggccg aaacgcggcg gttttgaatc
    11281 ataccaagag cgcgtggaag gcaataaagt tcgcgagcgc agcccatcgt ttggcgaata
    11341 ttattcccat ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt
    11401 cgatggtttc agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt
    11461 tgaccagctg gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat
    11521 cgaactgact gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa
    11581 ggatccatcc ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc
    11641 gattttactt aatgatgaag tgagatcggc agaccttctg gccattctca aggccatgaa
    11701 ggccaaaggc gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga
    11761 cggtacggtg ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc
    11821 ggtcattgtc ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta
    11881 tctgatggaa gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt
    11941 taaagcaaca atcaaggtag ctgaccaggg tgaagaaggg attgtggaag ctgacagcac
    12001 cgacggtagt tttatggatg aactgttaac gctgatggca gaacaccgcg tgtggtcacg
    12061 cattcctaag attgacaaaa ttccggcgta aatatggata aggtcacgtc ccctggggag
    12121 tgaccttacc accttactta cacatcaaga taactaccca accgataacc acgctcagca
    12181 attgcatatt tcaacgacgc tgatgtcagc acttccagct ccgttaaccg tgggaaacag
    12241 taagcactct gacggatagt attatcgata aacgccggat gacacatcac ctccagcgaa
    12301 ggatcacccc gatggcttgc gtcatccagc acttgcagga acagtgcctc gctaatctct
    12361 tcaccataaa acgcgctact gaatccctgc gaactgcgca gattagccgg taaatcacca
    12421 gcgttagata gcggctgacg gtcgatgcgc aacgcaatcc cctcttcagc cgcaaacctg
    12481 gcaacaatcg ggaaaatctg cgggaacata tgcacatgat gatggctatc aagatgcgta
    12541 ggtttgcgtc caaatagctc aatgaaacgc aaatactgac tggcaagctc ctgagtaatt
    12601 tcttccagcg gtaaagcatc ttcttctgcc aactgccaga tccattttcc cagcacacca
    12661 tcgcgggtta accccggcat agctgtcagt ggcttgccca tagtaaggac aaagtgcatc
    12721 cctatggcca gactcggttc atcacgactc aactgcaccg catggtcaat agcctgccca
    12781 ttcaccagcg ccgtcgtcga tgtgacaatc ccattgcgac aggcctcgat aatgccgtag
    12841 ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa tcagtaagcg ttccataatc
    12901 agcctcggtt aatgtgcttt tttaagctct gcgatgcagt cggcaaaatt tggcagccat
    12961 ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc
    13021 aacggactaa ggtttagcgc cagtaacaca tcgttaaatt ctccgctaag tgcggcgttg
    13081 ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa
    13141 tgcgtaatgc gcggatgtgg cgtcgcgcca tcgcgcccca gcttacaggt catttctacc
    13201 gcccagtctg ccggaatatt atcaatatgc ccatgatgcg ggatattaac gtaatgttca
    13261 gcttgcttgt cgttgtagat agcgttgatc acttcgcacg ctgcatcaga gtaataagcc
    13321 ccaccgcgct gttccagttc tttcggctta actttcaact ccggtttttt atacagctca
    13381 aaaagttgtt tctcgacttt ctgtactacc tgcgctcgtg cgccgccttt gtagtattcg
    13441 cccatttcaa tagccagcat ctctttttgc ttgaagtaat aaagcaaata agaacacggc
    13501 aacagattca gagaacgaat taagccttcg ctaaatggca gatcgaaaat atttttaacg
    13561 ccagatactt ttaactgccc ggatgccaca ccatcaagca attcggcaaa gcgcgacttg
    13621 ccatttacca gcacatcctt aatgaacacc atatggttga ggccaaacaa atcgatagat
    13681 aaatcatcgc tgtctttcag catcagaaca tcgtgaataa acatcttcat gccgatcgga
    13741 atattacaca cgccgataaa acgtttaaat ccggtatgac gataaacggc ttcagtaacc
    13801 attcccgccg ggttagtgaa gttaatcacc catgcattcg gacaaagttc ttcgacatct
    13861 tttacgatgt caaaaatcac cggaatggta cgcagacctt taaacaaacc gcccgcgccg
    13921 ttggtttcct gaccaagata accatgactt aatggaatac gttcatccag ttcacgcgcc
    13981 ggtaattggc caacgcgcag ttgggtagta acgaaatcag catctttcaa tgcttcgcgg
    14041 caatccagcg ttttataaag cttcatcggg acgccagcgt tatcaatcat ccgttggcag
    14101 agatcgaaaa taatatccag tttagctttg ccaccctcga catccaccag ccataattcg
    14161 ctgaccggca attcgtgata acgcttaata aatccttcca gtaactccgg ggtatagctg
    14221 ctcccgccac caatagtgac gacttttaat ttctggctca taatttctcc cttcagtaca
    14281 gaatactgat atctggcata tctgcccccc cggacataaa taatccagca acaggacaga
    14341 tatgtgaatt gtcaggtata acgacttact gattaaattc agtcaatttc ttacgatatc
    14401 tcttaggcgt aaaggacgtt aatttcttaa acgttttaat aaacaaactc gggctactat
    14461 aaccggcctc aaacgcaata tccgtcacgg aatagttggt catttccagt tgtttttttg
    14521 caaaattaat acgaatctca ttaataatct gcattggtgt tttgccataa tatcgttgag
    14581 tcgctcgcgt caaatattcc tgtgatttgg ctgacaacgt caccatattc tccagcgccg
    14641 attcactaaa ctgctcttta tcatgcatct tttctaccgt acttttcagc cactacggta
    14701 catcatcaat cacctgttct tcgcggtaat gacgtaaacg gttaatgaca taaaaagtaa
    14761 ccatctcaac aaactcttcc agccctgttt cgcggaaatt taatgaagaa atcactgttt
    14821 ccacataggt gagaaaagcg ttattggtcc ggtatacctg cgaagcaaca aagcaataag
    14881 gcaacaacgg caggtaatgc tgctcaaaaa agcgtttact gatcccaacg ttcaatatgc
    14941 gcgtggcacc aaactcataa aaactttgat ggtgcgaacc taacggaata aaaacaaaat
    15001 cgccccgttc cagtaacacg cgcttaccgt taatctccta gaaataacgc ccggttaata
    15061 ctagagtaaa ttcataatag tcgtgctgat gcagtccgct gatactctca gttttgttat
    15121 agataaacac atggaagttt ttgccattaa acaactgctg ttctcgggca gtggcaattt
    15181 ccggcgcgtt aatcactggc tgcatcatcg actccttatg ccttcagttt ttcatgaagc
    15241 tcaattaatt cagtaatcag ttcacgcgca agcatggacg tcattaaatg atcctgagcg
    15301 tggaccagca ccagactcac tttcatctta ccttcgcccg catcgccttc aatcagtttc
    15361 gtctgtacca gatgcgcttc attcaatgcc atccgtgact gatccatcat ggctttcgct
    15421 gcggcaaaat cgccctgctt cgcctgtttc aatgcggcat acgccaggct acgcgcttgt
    15481 ccagagttga tgataagccc catcaccact tcttccagct cttcagcttc tgtttgcgta
    15541 tcaggaatat tatcgagatc catcatacat cgttcctctc tcttcttacc ggcaagatta
    15601 cccgtaccgg catcgattaa aatttcaggg gttagcgata tcttcttcgc tctcttcttt
    15661 atcaatcgca ttttgtgctt tgttagccac cacaacaaag ggcagataaa ttaacgttgc
    15721 gatgccaagg ttgaagagtg cgaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa
    15781 ggctcccaga ccggttggca tggtccacgg tgcaatattg gtcaccggag gaatgatgcc
    15841 catgtagtac gctgcgaggg tgattgccgc cagaatcggt tgtaccagta caaacgggat
    15901 aaacatcacc gggttcatga taattggcag accaaacaga atcggttcgt taatctggaa
    15961 gatgccggac ggcagcgcca gttttgccac ctgacgataa tcagcacggc gagaggcgat
    16021 aaagatagcc aggatcaggc ctaaagtcgc accactgccc ccaaggaaaa taaaggagtc
    16081 cagcatcggc ttggcccaga tatggaaggt cttaccggct gccagcgccg cttcgacgga
    16141 accatattgc tgataggtcg cgatattttc cagcgcccac ggcgtcataa tgccgttgtc
    16201 cagtgcggtc agcgccagcg cgccatgaat accgaagaac cagagcagtg gaacaaagat
    16261 cacataggcc cagcccacca cgctacccaa cgatgccagt ggggttgaga tggtatccat
    16321 aatgatctga tggaagttgg tgccccaggt attcaacgcc caggcaataa tccccatcac
    16381 ggaaagaata ataaagccgg gaattaatgc cgagaaagaa cgcgatactg aagcaggtac
    16441 gctgtcgggc agtttaatga cccaattgcg gcggacaata aaggtaaaca tttctgccac
    16501 caccaggcca ataataatcc cggagatgat attcgcccca cctaaccagt ttgcgccaac
    16561 cgcataggcc tcaccgacac tatatggggt gacggtcata aatgctgcaa cggataacaa
    16621 cccagccgcc agcgcatcga ctttacgctc ttctgccagc gccatgccaa taaagaacgg
    16681 tgccattaaa gacattattc ctaatgttcc gttatatacg ttgccgccaa tacctttcag
    16741 accattaagt gtttcaatgg ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga
    16801 cccctcccca aagcttagaa aaacgttgtt aattaataca aacatggccc ccgcaagggt
    16861 taacggcatt aagcgaataa agccattttt gattgcatta acgtgtggct gctttcctat
    16921 tttaactgca aaagggagga gtaccttttc aagcgatgca ataacattac tcatagaaaa
    16981 atacccttaa aaaccgcaat ttaaatatta cggtattgat ttatgaaata actctttgac
    17041 gggaaaattt aaaataaatt aatttgctgc ggctttttta atcgctgcaa ccgcagcctt
    17101 aagcacgcct aaaccatcga ctttgccata aagcagcgag tcaattactt caaccggttt
    17161 gttgggtaac aaacgctgga tttcgggcaa catataagca atctgcggcc ctaataacac
    17221 gacatcggca ttctgacctt tttcaccagc cagtgtttcc ggaaatgctt caataatgac
    17281 cggaacttca tatttttctg cctgtgcgcg catttttgat accagtaaag agatagacat
    17341 gcccgcagaa caaaacagat aaatgtgttt cttttccata aaactgccct catcgacgat
    17401 tatctgtcag ccagacactc cgcaagcctt gacctgcttc catgctctgg gtaacttgcg
    17461 aaaccaacat gacttttttt gtggctgaaa ggagtatacg gtatagacct gagcaatagt
    17521 atttctttga ccttataaat tgtttctcct tgacctgtgg ttatgaccct cttcacattt
    17581 cgggcaaata attcgcgaag ataattaaga actcaggcca aaaaaaaccg gcgcaatggc
    17641 cggtttccgt tgttactcaa gctttcagac gaattgatta cttcgcagcc tgtggatcag
    17701 tgtcgtattc agcacaggtc tgataaccgg agttgatgac atgtccggta tcatctaacg
    17761 cgacaaagta ggtttctgct ttaccatcac gttgacccag gatgtaggtc tggcaagtac
    17821 cgcgagcatg gatcatgctc acttcagacg aaggtttacc cgcaatttgt gcaacctgcg
    17881 cccggctcat gccttttttg acgtctttca ccacaggctg tacaaactgg tctttggtac
    17941 gatcataagc tgtacaaccc gccagcatgg ttaataccgc cgctgcactc agaattcctg
    18001 ccatattctt gttcatattc cgtcctcttg tttatcagcg tgttagataa gcctggaata
    18061 cattgggcgc tttttcaagc ccgtgaacga aacggctccg ctttcagagg attcctgtat
    18121 gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct
    18181 ttaaggaagt tttgtctttt ctgtctggag gggttcaatg acattgcaac aacaaataat
    18241 aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag gaaattcgtc gtagtgtcga
    18301 ttttctgaaa agctacctgc aaacttatcc gttcattaaa tcactggtgc tcgggatcag
    18361 cggcggtcag gactccacgc ttgccggaaa gctgtgccag atggcgatta atgagctgcg
    18421 ccaggaaact cgcaacgaat cactgcaatt tattgccgta cgcctgccct atggtgttca
    18481 ggctgacgaa caagattgcc aggatgccat tgcctttatt caaccggatc gcgtattaac
    18541 cgttaatatc aagggcgcgg tattggctag cgagcaggca ttgcgggaag caggcattga
    18601 actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag
    18661 cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat catgcagcag aagccattac
    18721 cggattcttc actaaatatg gtgacggcgg tacggatatt aatccgctgt atcgtctcaa
    18781 caaacgccag ggtaaacagt tactgacggc attaggttgc ccggaacacc tttataagaa
    18841 agccccaact gccgatcttg aagatgatcg cccttctctg ccagatgaag tggcactcgg
    18901 cgtgacctat gacaatatcg acgactatct ggaagggaaa aacctacctg aacaggtcgc
    18961 cagaacaata gagaactggt atctgaaaac cgaacataaa cgccgtccgc caattaacgt
    19021 tttcgatgat ttctggaaaa agtaataatt tgcacctatt ggattgtgcg aaaaagagta
    19081 atttgttcac gccggatgcg gcgtgaacgc cttattcgac ctacaaaact atgcaaattc
    19141 aatacattgc aggagtcgaa taggcctgac aggcgtagca cgtcagacgg tgtaaccttt
    19201 gtcatcaacc cgcctctttt ttaatcgcct cccgcctgtt acactggata gataaccagc
    19261 attcggagtc aacagtggta cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg
    19321 caatttatga atatcccgaa catttacatt cattccttaa tgacttaccc acccgaccag
    19381 gggtgtatct gtttcatggc gaaagtgaca ccatgccgct ctatatcggc aaaagcgtta
    19441 atatccgcag tcgggtgctt tctcatttac gcaccccgga tgaagccgcc atgctacggc
    19501 aatcccggcg aattagctgg atctgcaccg caggcgaaat cggcgctctg ctccttgaag
    19561 cgcgattaat caaagaacaa cagccgctgt ttaataaacg gttgcgccgc aatcgccagc
    19621 tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt gtatgccaaa gaggtggatt
    19681 tttcacgagc ccccaacctg tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat
    19741 tgcagagcat cgctgatgaa caaaaacttt gttatggcct gttgggactg gaaccgttaa
    19801 gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg cgccggagca tgctgcggta
    19861 aagagagcca tgaggaacat gcgctacgct tgcgccagtc tctggagcgt ttgcgagtgg
    19921 tgtgttggcc ttggcaaggt gcggtggcac tgaaagaaca gcacccggaa atgactcaat
    19981 atcatattat tcaaaactgg ctgtggctgg gggcggttaa ttcgctggaa gaagcgacaa
    20041 cgttaattcg gacacccgcc gggtttgatc acgacggtta taaaattctt tgtaagccgc
    20101 tgctttccgg taactatgaa attactgaac ttgatccggc gaatgaccag cgagccagtt
    20161 gatttcactg aacaacagtt taccttgcgg ttgtaaaagc cgcagggtgt gcctgccatc
    20221 gaaccagcac gcgccctggt cggtagtgag cagtttgtcg cccagttgcc acgccccgtt
    20281 aatcacaaac accaccccgc cgcgtgagcc aaaggtagta aacgtacgtt cggcaattct
    20341 gactttcgcc ttacagacat ccaggcgcgt cataatattg aaatccatcg acatctgccc
    20401 tgccgtcagt ttcgctttta ccacctggtc cgccgtaaag gcaaaaggct gtaacggttt
    20461 taaggtatgg ttaaagcggt ctgcgctttc aaggagcatc tcgccgcctt ccagcaacgt
    20521 cactatcctt tccatgccgg gaaacaaaga aaactcccca ttcgccgcga tggatgcaat
    20581 gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa gtgcaaattt cgcgcgtttc
    20641 accggcagcg tttcgccaca gattcaccga cattttacgc atatcaaagt attccatact
    20701 cgctcccggc ccgttctttt cattgttatc gttcaggtaa ccgtggatgg ttatcggcaa
    20761 ctcgggtgat ttacgttagt ggtgatcagg ctttttttac aactgtttga gaagagaaaa
    20821 gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag tggacaagac cggcggtctt
    20881 aagttttttg gctgaaagat tattcagcag ttgcaggcat tttacctttt gccgctggac
    20941 gttctgtcag acgcttctca aaattagcat taaattgctt tttctgttcc ggcgtcagga
    21001 tgttgtaaat tttgttctgg gtttccatgt gcgccagcat gttagctttg cgctgttctt
    21061 ccatttttgc gatctgcgct tcagcttttg ctttatcgaa ggtatcgctg gcaatgatgt
    21121 catgcattgc gcggcgttct tccagcggcg gacgtttcat ctggtcacgc tggcctttca
    21181 tgatttcgtg gatctgctgt ttctgcgcgt cggtcaggtt caggtctttg aacatcatgt
    21241 cctgatgcgg accgaacttg cctttgtggt gcatcatcgg cttcgcgtca gccggtgctg
    21301 cggtagtggt gtctgcggca tgggccaggt tagccgcgcc aagagccagg gtagaggcaa
    21361 caaacagtgc agttaattta cgcatattct atatccttcc tttcagttat ttattacggc
    21421 tttcttaagt agcgtgccgt gttgacgaga ttaactttac tgactttagc gtcaattaat
    21481 cagagcaacg gtaaaacaat gaaagtgtaa aaaacacttt tgcgccaatt atggagaaaa
    21541 aaagaaaatt tgatggagag tgatgagaga atattacaac acgatgattt tgcagagatt
    21601 atgaagaact ataccggatg actggtgata aataaagcaa ataaccagga ttaatctgta
    21661 ttaatttata agaaagcaac ttaatacccg cagaatgatt tctgcgggta agtattagct
    21721 tattttttcg agcattaatc ccgcgcgtaa tcccaacgct accaacggat tagggaataa
    21781 cacatactct acatcatggg ttacggtaaa acgttcctct ccgtcctgcg ccagcaatgt
    21841 tcctttctca aacggcataa aattcagcgt gtcacttgcc atatgcattt cgaaggacgg
    21901 cgagtggcga gtaatttgcg aaaccacccg ataacggagc ggcggtgttc tcacgatacc
    21961 gacactctca ccagatagca gcgcagcaat tgcgctggca gttactgcaa actggcgaag
    22021 atcgttttgc ccaaagggca acgctttgcc aagttccaac gtacaggcca gcgcgccaaa
    22081 atgtctgacg ctgaaatggg taaacgtacc accaggctcc tgatggaaca ccagcgcctc
    22141 cagccccgcc gcacccagcc acgtcagaaa tttctcgtcc cagagaatgt cgcgttgcgg
    22201 taatacaccg aactgcggat gcaaggagcc acgaattgcg gtatgtagat caaggtgcca
    22261 acgcacagat tctttgccct ggtcataaaa atcttccagg cactgttcca gttcgcgcgc
    22321 ccgacaggtt tctccgcttt cagcaaatag ctgccaacga ccgccaaaca ttcggttcat
    22381 atcgctatgg caataacgtt tcccttgctt cagcgcagga ggattcccga ggatcaccag
    22441 caaccgccaa cgtaacggga tctcgccgtg agatatcgcg ccaagcaacg cgtccagcat
    22501 ttccacaggt gccgtctcat taccgtggat ccccgcagaa atcaccagcg cgccttgcgg
    22561 tggcgttaat ggcgtcagtt ccagcacacc atcgcccagc cagcgccagc gaacgccgtt
    22621 gatttctcgc tcggtgataa ccggtttttt acccgttaag gtcagagcaa gaaaattatc
    22681 cattgccccc tccctcgcgc tggaacggat aaaccgaacc gagattcagt aattggctca
    22741 atacatccag tgcttcccgc ccttcgcgca gcaattgcgg gtcggccaga tcggcagcag
    22801 taagacgatc gcggtagtaa cgatccaccc agtcattgag cgcattaaac aaggtatcgt
    22861 tcatcatcac cgccggattc accgcccggc gttcttcttc tgtcaatacc acccgcaacc
    22921 gcaggcacgc cgggccgccg ccattcgcca tgctttcacg taagtcaaag acttttagtt
    22981 cgctaatcgg gttgtcagcg gcaaggagtt cattgagata accccatact ccggcgtgtt
    23041 cccgacactc ctgaggcagc accaacatca tggaaccatc atcgcggctc aacagttggc
    23101 tgttaaacag ataggtagac accgcatccg acacggaaac ctgagttgcc ggaacttcta
    23161 tcgccataaa accgttcacc cgcgtgccag taactgtgac tggcgagcga acgcctgttg
    23221 gtggcaaaac agcacctggc ggttactcac ggcaatcacg tcattatgaa aaacgctctg
    23281 gtcgataacg tccgggtttt gctgggcgaa aatcacctgt tggggattca cctgattaag
    23341 ccttgccacc acctcgctgg cttcgcgagt ctgtcgcgcc ggataacggg aaggccgggt
    23401 atcattgcct tcttctcgcc cgtagacaaa aagtagcata cccggttcac cgtaatgacc
    23461 gccgagacga ttgtggtttg ccgccccctc atcaccgagc aacgctacct gcggcaacgc
    23521 cgaatggacg ctaaatttct cttcgtcctt aaaaatcgct tttaacagcg attcagtgac
    23581 gggcgcttcc agcgaacggt gaaatttatt gttcaggttg gcaacggtga gatgcacttt
    23641 gccatccagc gtatcggcag atggcgcgat cgttgccgca ttggctaccc acattggcga
    23701 agcggaactg acgctggaaa gccagtgcgg tgcctggcgt gcaacttttt ccagtacctg
    23761 ctcatcgcta ccactgaatc ccaactgacg cagcaccgga ataaacggac gctcgtgcgg
    23821 cgggatcacg gcctggggga atcccgcatc ggcaagggct ttcattttca gtaagccctg
    23881 ttttgccgcc agtcgcgggt tagacacctg aaaacggtga cgggtagagg cttcattacc
    23941 aaacgacagg cccgcgtaat gatgcgtcag ccctaccagc ccgtcgaaat taacttccca
    24001 ggcgttcatc gcaccacctc atcggaaaaa tccagcccgg ggttaagcgt ggcgggcaat
    24061 gttaacgagt ccgactccag gctcgccata ggccatgcgc agtaatctgc ggcataccag
    24121 gcgctggggc gatggttacc ggaagcacca atgccgccga atggggcggt actggcagca
    24181 ccagtaagcg gtttgttcca gttaacaatc cccgcccgcg cctccagcaa cagttgatcg
    24241 aatttttccc gctcggggga aaccagaccg caaaagagtc cgaagcgagt gttgttcgcc
    24301 atccgaatcg cctcatcaaa agtatcataa cgccagacgc gcaaaagcgg cccgaacacc
    24361 tcttcatccg gcacaccgcc aacgcccgtc atttcaatga tccctggtgt cagcaacgat
    24421 gtccctgctt gtaataagcg cggcgcaagc agggttcgtc cgcccatcgc ttccagttgc
    24481 tgccaggcag taaccacctg ctgtgcggcc tgttcagaaa tcagcccgcc aataaacggc
    24541 tgcggttcgt catcccagtt gcccggcgtt aatcgctggc tgacggcaac cagactagca
    24601 agaaacgcat cgccctgcgc tccgcttttg agcaataaac ggcgggcgca ggtgcagcgt
    24661 tgcccggctg tgacaaacgc cgactgaatg gtcagatgga cagccgcgtc gatatccgcc
    24721 acctcatcga taattagcgg attattaccg cccatctcaa gggcgagaat tttctccggc
    24781 tgaccggaga gctggcgatg cagctggtag ccggtattgg cgctaccggt aaacagcaaa
    24841 ccgtcgagat cctccagcgc actcagcgcc tgacccgttt cacgcccgcc ctgcaccagg
    24901 ttcagcacgc ccggcggcaa gccagcctgc tgccataaac gcattaccgc ttcgccactc
    24961 cacggtgtca gttcgctggg tttaaagata atggtgttac ctgccagcaa tgccggaacg
    25021 atatgtccgt tcggcaaatg accagggaaa ttatacggcc caaacaccgc cagcacgccg
    25081 tgcgggcgat gtcgcaggct cgccgcaccg tccggcattt cactacgctg ctcgccggta
    25141 cgaacgtgat acgccttaat tgatatcgcg attttattga tcatcgccgt cacttcggtt
    25201 gccgcttccc agcgcggctt acccgtttct ctggcaataa tcgcggttaa ttcggcttta
    25261 ttgctttcca gcaatccggc aaagcgttcg acaacgacct gacgttcagc caatgagagc
    25321 cgcgcccagc gcggaaacgc cgcacgggct gcccgacaag cctgttcgac ctgagcggca
    25381 tcggcatcat tgccttgcca taacacctcg cccgataccg gattacgctt cacacgcgat
    25441 gcgccctggc ccgttatcca gtcaccgtta atccataaag tcatgctgtt ttctcctctg
    25501 cgcacaggcg caccagacga acgcgatccc ctgcgtggca tttgagggca tccagttgtg
    25561 cggcggttaa aatcaaacgc tcggttgccg gatcggtacg cgccagcacc acgcggaaat
    25621 ggtgataatt ttcattggcg accaggcagg ccggaaaatc gccctgcgca ggctgccctt
    25681 ctgccacttc caccagccga cttttacgga tggcgcgcac gcggtcgatg tcacactcaa
    25741 gcgttggccc gccgtcgaag atgtcgatat agttgcggta acgaaaacct tctttctcca
    25801 gcaccgcgcg ggcaggcgcg gtttgcggat gtacctgacc gatgacgtcc tgggcttcct
    25861 gggataaaaa gtgggtatag atcggatgtt tcggcatcag ttctgcaata aatgcctttt
    25921 gcccggtgcc gcagagaaaa tcggcgcggc taaaatccat cgaaaagaag cgtttaccga
    25981 ggctttgcca gaacggtgaa tagccgtgtt cgtcaatcac cccgcgcatt tcagcaacca
    26041 ctttgtcatt aaacttgtcg cgaaaagccg ccataaacat aaagcgcgat ttcgacagca
    26101 aatagccgtt gccctctttg cgccagtccg ggtcgagaaa cagcgtgcac agctcgctgc
    26161 tgccggtgtg atcgttacta agaaacagcg tcggcaatgc gttatagaca ttcagctctt
    26221 ttgaggcgtg aaccaacgtg ccgacgcgat agttgtacca gggatcgttc agcccaaccg
    26281 ccacctcaat agcgcaaatc cccgccacgg tgcctgtctc gctatcttcc agcacgaaca
    26341 catagccctg ctcgcttttg ggcagttcac cttgccaggt tttgattgcc ctttcgatac
    26401 gcgccgaaag cgtggcttca ttggcgggaa gcgacgtcag gccgccgccc gttttgctgg
    26461 caagctgcat cagcgccgag acatctgagc gctcaacggg acggatgacc atcatgatga
    26521 acctcggcta acaaagtgtt cgcaagcagc tgcaaagcga tccagtccgg tcgtcacctc
    26581 ttcttcgctg acattgagcg caggcgcaaa acgcaccacg ttgccacccg caatcagtac
    26641 catcacgcct gctttcgccg cttccagaga gatctgtttc gcttgcccgg cgtaatcggc
    26701 attcagtaca cagccaatca acaaacctaa gccgcgaact tcactgaaca aaccatagcg
    26761 gtgattaata gtattaagac gctcaacaaa ccagtcgtga cgctgtttaa cgccattaag
    26821 catctctggt gtgttgatga gctccagcac tttgcctgct accgccgagg ccagcggatt
    26881 accgccatag gtggtgccat gagtgccaac ggtcatcacg cgggcgcact cttcggttgc
    26941 caacaacgca ccgaccggga aaccgccgcc cagcgctttg gcggtggtta acagatcggg
    27001 cgtcacgccg tagtgcatat aggcatacag ctcaccagtg cgcccgacgc cggtttgtac
    27061 ctcatcaaaa atcagcaagg cattgtggcg gtcacacaat tcacgcagac cttgtaaaaa
    27121 cgcgttgctg gctggcacca caccgccttc gccctagatg ggctcgacaa tcaccgcaca
    27181 ggtggcgtcg tcaatcagcg cgctggcaga gttaatatcg ttatatgcag catgacgaat
    27241 atccgccggc agtggcgcaa aatcctgtga ataggctggc tgcccacccg cactgacagt
    27301 aaacagcgtg cgaccatgaa acgcattttt gaacgccacg atgccactct tatggctgcc
    27361 gtagcggtcg tgagcgaatt tacgcgccag ttttagcgcc gcttcgttgg cttccgcacc
    27421 ggagttacaa aagaagacgc gatcggcaaa cgtggcgtcg atcaattttt tcgccagtcg
    27481 cagtaccggc tcgttggtgt aaccgttgcc ggtatgccag aacttactcg cctgttcgtt
    27541 cagcgcttca cgcagttccg gatgcgcatg gcccagcgcg ttcaccgcaa tgccacccgc
    27601 gaagtcgata tactctttcc cctgctgatc ccacaagcgc gaaccttcgc cacgtaccgg
    27661 tataaagggt gccggagcgt aaacaggtat catccattca tcaaagtttt cacgcgtaat
    27721 tggctgagac atagcgacct ctacagtaaa taattcgtta tttgtatgtt aacaataagt
    27781 aatgtttgcg ctgtaaatgt agattgcagg gttcgtgcca gccagtgata aaagtgcata
    27841 aacggcggag gctaactgga aatcaaggag ttataaccaa accatatgca tttaaagtgc
    27901 atataaagtg aatacgtttg cgatgtgggt gaataaaaag aataaaaaac gcaatgttat
    27961 gcagaagtaa aatataattc tagaattgtg atcattgacg aaatttactg gaaattactg
    28021 cgccattctg acgcagcgcg caccaaaagc gggcattttt tgcgccatcg ttgacatcat
    28081 taacaaccat cgatcaaatc acttaacaac aggcggtaag caacgcgaaa ttctgctacc
    28141 atccacgcac tctttatctg aataaatggc agcgactatg aaatttgtct cttttaatat
    28201 caacggcctg cgcgccagac ctcaccagct tgaagccatc gtcgaaaagc accaaccgga
    28261 tgtgattggc ctgcaggaga caaaagttca tgacgatatg tttccgctcg aagaggtggc
    28321 gaagctcggc tacaacgtgt tttatcacgg gcagaaaggc cattatggcg tggcgctgct
    28381 gaccaaagag acgccgattg ccgtgcgtcg cggctttccc ggtgacgacg aagaggcgca
    28441 gcggcggatt attatggcgg aaatcccctc actgctgggt aatgtcaccg tgatcaacgg
    28501 ttacttcccg cagggtgaaa gccgcgacca tccgataaaa ttcccggcaa aagcgcagtt
    28561 ttatcagaat ctgcaaaact acctggaaac cgaactcaaa cgtgataatc cggtactgat
    28621 tatgggcgat atgaatatca gccctacaga tctggatatc ggcattggcg aagaaaaccg
    28681 taagcgctgg ctgcgtaccg gtaaatgctc tttcctgccg gaagagcgcg aatggatgga
    28741 caggctgatg agctgggggg tggtcgatac cttccgccat gctaatccgc aaacagcaga
    28801 tcgtttctca tggtttgatt accgctcaaa aggttttgac gataaccgtg gtctgcgcat
    28861 cgacctgctg ctcgccagcc aaccgctggc agaatgttgc gtagaaaccg gcatcgacta
    28921 tgaaattcgc agcatggaaa aaccgtccga tcacgccccc gtctgggcga ccttccgccg
    28981 ctaatttagc agctctcctg gctcaaactg ggtcaggaga attaaccttg agaaaaatca
    29041 acaaactgtc agtaatgatt tgttgcctgc cgtcctttgt tataccgtca ctgcgttttt
    29101 agttgtctga ccacttctct attatcaagt ttgatatagg aaactccacg atgaacgctg
    29161 agcgtaactt tctgtttgcc tgtcttattt ttgcgctggt catttacgct atccacgctt
    29221 tcggtttatt cgatctgctc accgatttac cccacttaca gacactcatt cgccagagcg
    29281 gattgttcgg ttatagcctc tatattctgt tattcatcaa tgccccctct ttctgttacc
    29341 aggaagcata ctggtgatcg ctggaggaat agtttttggc ccgctcttag ggacacaact
    29401 ctcattaatt gccgcaacgc tggcctcctc gtgctcattc ctgctggcgc gctggctggg
    29461 acgtgattta ctgctgaaat acgtgggcca tagccatacc ttccaggcca ttgaaaaagg
    29521 cattgcgcgt aacggtattg attttcttat tctgacccgc ttaatcccgt tgtttcctta
    29581 caatattcaa aattacgctt acggattcac caccatcgcc ttctggcctt atacccttat
    29641 ttcggcactc acgaccctgc ccggtattgt tatttatacc gtgatggcaa gcgatctcgc
    29701 caatgaaggc attacgctgc gctttatttt acaactctgt ctggcgggcc tggcgctgtt
    29761 tattctcatc cagctcgcaa aactctacgc ccgacacaaa catgtggatc tgtctgcttc
    29821 gcgccgcagc ccacttactc acccaaaaaa tgaaggatag aacgatgtcg caacattatt
    29881 cagtgtcatg gaaaaaagga ctggctgcac tctgcttact ggctgttgcc gggcttagcg
    29941 gctgcgatca aaaagagaat gcggcggcaa aagtggaata cgacggactt tcgaacagcc
    30001 aaccgctgcg cgtcgatgcc aataaccata cggtaaccat gctggtgcaa attaatggtc
    30061 gtttcctcac cgacgacact cgtcacggta ttgtgtttaa agatggctcc aacggacata
    30121 aatcgctgtt tatggcttat gcgcccccga aagcatttta tgaagccctg aaagaggcag
    30181 gtggtacgcc gggcgaaaac atgacgatgg ataataaaga aacgactcat gtcacaggca
    30241 gcaaactgga tatttcggtc aactggcaag gggcggcaaa agcgtattcc ttcgatgaag
    30301 tgattgttga cagtaatggc aagaaactgg acatgcgctt tggcggtaat ttaacggcag
    30361 cagaagagaa gaaaacgggt tgcctggtgt gtctggatag ctgcccggtc ggcatcgtca
    30421 gcaatgcaac atacacttat ggtgcggtgg aaaaacacgg cgaagtgaaa ttcaaaggca
    30481 atgcctcagt tctcccggcg gataacacgc tggcaacggt tacctttaaa atcaccgaat
    30541 aaagccaaga taaaggatga tgaagataca atcgcgtaaa atctggtact accgtataac
    30601 cctcatcatc ctgttgttcg ctatgctgct cgcatgggcg ctgcttcccg gcgtccatga
    30661 gtttatcaat cgcagcgttg cggcgtttgc cgccgtggac caacagggta tagaacgctt
    30721 tattcagtct tacggcgcac tggcggcagt tgtctcgttc ttgttgatga ttttacaggc
    30781 cattgctgca ccgctacctg cgtttttgat cacctttgcc aatgcgtcgc tgtttggcgc
    30841 gttctggggc ggcttgctgt cgtggaccag ttcgatggcc ggcgcggcgc tgtgcttttt
    30901 tatcgccaga gtgatgggcc gcgaagtggt ggaaaaatta accggcaaaa ccgtgcttga
    30961 cagtatggac ggctttttca ctcgctacgg caaacacacc atcctggtct gtcggttatt
    31021 gccttttgtc cctttcgatc caatcagcta tgctgccggt ctgacttcaa tacgttttcg
    31081 ctcgtttttt atcgccaccg ggcttggtca attaccggcg actattgttt attcctgggc
    31141 aggcagcatg ttaacaggcg gtactttctg gtttgtcacc gaactgttta ttctgtttgc
    31201 cctgaccgtg gtgattttta tggcgaagaa aatatggctt gagcgccaga agaggaatga
    31261 ctgatgggtt taccgccgct tagcaaaatt cctttcattt tacgtccgca ggcgtggctg
    31321 catcgtcgcc attacggcga ggtgctaagc ccgattcgct ggtgggggcg gatcccgttt
    31381 atcttttatt tggtgtcaat gtttgttggg tggctggagc gcaaacgctc accgctcgat
    31441 ccggtagtgc gatcgcttgt cagcgcgcgc attgcgcaaa tgtgcctgtg tgagttttgc
    31501 gtggatatca ccagtatgaa agtcgccgag cgtaccggca gcaccgataa actgctggca
    31561 gtggctgact ggcggcaaag cccgctcttt agcgatgaag aacggctggc gctggagtac
    31621 gccgaagccg caagcgtcac accgccaaca gtcgatgatg ccctgcgtac ccgactggct
    31681 gcgcattttg acgctcaggc gctcaccgaa ctgacggcat tgatcggcct gcaaaacctg
    31741 tcggctcgtt ttaattctgc catggacatt cccgctcagg ggctgtgccg tattccggaa
    31801 aaacgttctt aaggagggaa gatgcgccat tgtgggtggt tgctgggatt gttatcgctg
    31861 ttttctttgg caacacatgc cagtgactgg caggaaatta aaaatgaggc caaagggcaa
    31921 accgtctggt ttaacgcctg gggcggcgat accgcaatta accgctatct cgactgggtt
    31981 agcggcgaga tgaaaaccca ttacgctata aacctgaaga ttgttcgtct cgcggatgcc
    32041 gcagacgcgg tgaagcgcat tcagaccgaa gctgctgccg gtcgtaaaac gggcggctcg
    32101 gtggatctgc tctgggtgaa cggcgaaaac ttccgcacct taaa
//
LOCUS       DAOFMX010000046        31904 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.046, whole genome shotgun sequence.
ACCESSION   DAOFMX010000046 DAOFMX010000000
VERSION     DAOFMX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.046"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(97..1485)
                     /gene="yeaG"
                     /locus_tag="QBA20_002358"
                     /pseudo
     CDS             complement(97..1485)
                     /gene="yeaG"
                     /locus_tag="QBA20_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
     gene            1921..2667
                     /gene="mipA"
                     /locus_tag="QBA20_002359"
     CDS             1921..2667
                     /gene="mipA"
                     /locus_tag="QBA20_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDQ6292867.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2757..3518
                     /locus_tag="QBA20_002360"
     CDS             2757..3518
                     /locus_tag="QBA20_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDQ6292868.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3569..4453)
                     /locus_tag="QBA20_002361"
     CDS             complement(3569..4453)
                     /locus_tag="QBA20_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDQ6292869.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4537..5532)
                     /gene="gapA"
                     /locus_tag="QBA20_002362"
     CDS             complement(4537..5532)
                     /gene="gapA"
                     /locus_tag="QBA20_002362"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDQ6292870.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5874..6287
                     /gene="msrB"
                     /locus_tag="QBA20_002363"
     CDS             5874..6287
                     /gene="msrB"
                     /locus_tag="QBA20_002363"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDQ6292871.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6329..6601
                     /locus_tag="QBA20_002364"
     CDS             6329..6601
                     /locus_tag="QBA20_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDQ6292872.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6971..8047
                     /locus_tag="QBA20_002365"
     CDS             6971..8047
                     /locus_tag="QBA20_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDQ6292873.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            8074..9453
                     /locus_tag="QBA20_002366"
     CDS             8074..9453
                     /locus_tag="QBA20_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6292874.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFISNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9470..10515
                     /locus_tag="QBA20_002367"
                     /pseudo
     CDS             9470..10515
                     /locus_tag="QBA20_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10536..11372
                     /locus_tag="QBA20_002368"
     CDS             10536..11372
                     /locus_tag="QBA20_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDQ6292875.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11377..12324
                     /locus_tag="QBA20_002369"
     CDS             11377..12324
                     /locus_tag="QBA20_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDQ6292876.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12334..13314
                     /gene="ydjG"
                     /locus_tag="QBA20_002370"
     CDS             12334..13314
                     /gene="ydjG"
                     /locus_tag="QBA20_002370"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HDQ6292877.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13451..14209
                     /locus_tag="QBA20_002371"
     CDS             13451..14209
                     /locus_tag="QBA20_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ6292878.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14327..15685
                     /locus_tag="QBA20_002372"
     CDS             14327..15685
                     /locus_tag="QBA20_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6292879.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15778..16419)
                     /gene="pncA"
                     /locus_tag="QBA20_002373"
     CDS             complement(15778..16419)
                     /gene="pncA"
                     /locus_tag="QBA20_002373"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDQ6292880.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16430..17446)
                     /gene="ansA"
                     /locus_tag="QBA20_002374"
     CDS             complement(16430..17446)
                     /gene="ansA"
                     /locus_tag="QBA20_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDQ6292881.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17613..19469)
                     /gene="sppA"
                     /locus_tag="QBA20_002375"
     CDS             complement(17613..19469)
                     /gene="sppA"
                     /locus_tag="QBA20_002375"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDQ6292882.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19630..20181
                     /locus_tag="QBA20_002376"
     CDS             19630..20181
                     /locus_tag="QBA20_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDQ6292883.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20298..21341
                     /gene="selD"
                     /locus_tag="QBA20_002377"
     CDS             20298..21341
                     /gene="selD"
                     /locus_tag="QBA20_002377"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDQ6292884.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21346..23307
                     /gene="topB"
                     /locus_tag="QBA20_002378"
     CDS             21346..23307
                     /gene="topB"
                     /locus_tag="QBA20_002378"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDQ6292885.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23434..24474
                     /locus_tag="QBA20_002379"
     CDS             23434..24474
                     /locus_tag="QBA20_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDQ6292886.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24591..25930)
                     /gene="gdhA"
                     /locus_tag="QBA20_002380"
                     /pseudo
     CDS             complement(24591..25930)
                     /gene="gdhA"
                     /locus_tag="QBA20_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
     gene            26166..26438
                     /locus_tag="QBA20_002381"
     CDS             26166..26438
                     /locus_tag="QBA20_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDQ6292887.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26404..26811)
                     /gene="nudG"
                     /locus_tag="QBA20_002382"
     CDS             complement(26404..26811)
                     /gene="nudG"
                     /locus_tag="QBA20_002382"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDQ6292888.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26898..27527
                     /locus_tag="QBA20_002383"
     CDS             26898..27527
                     /locus_tag="QBA20_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDQ6292889.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLTFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27536..28843)
                     /gene="ynjE"
                     /locus_tag="QBA20_002384"
                     /pseudo
     CDS             complement(27536..28843)
                     /gene="ynjE"
                     /locus_tag="QBA20_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28910..29563)
                     /locus_tag="QBA20_002385"
     CDS             complement(28910..29563)
                     /locus_tag="QBA20_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDQ6292890.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29563..31080)
                     /locus_tag="QBA20_002386"
                     /pseudo
     CDS             complement(29563..31080)
                     /locus_tag="QBA20_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(31053..31904)
                     /locus_tag="QBA20_002387"
                     /pseudo
     CDS             complement(31053..31904)
                     /locus_tag="QBA20_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7998 a         7640 c         8271 g         7995 t
ORIGIN      
        1 cgtcactctc gtctggggaa tatctccccg gcagccttca gggaaaaata tcatcagatg
       61 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct caatctgctg
      121 ttccaggacg tagaacagat ggaccgggtt tgccgccact tctacatgat cgaagttgaa
      181 cacgcgggag aggatcttaa acgcaaaacg cgtcgacaga ccgttcatcc cttcatcgac
      241 accggcgtag tcacgatcgt agtcacgata ttcctgatac gacttggctt tcggatcggt
      301 gtctttcaga ctttcgccat cataaacccg catctttgaa taaatgctgg agttttctgg
      361 ctctttcagg cgcgaaagaa tggaaaaacg tgacagtgtt tcgagcgtgc caggggcgca
      421 tggggcgtga gtcaattcac tgtgattaag caatttctcg tagattttga tctcttcgga
      481 aatgcgcaag caatacggca ccttcacgat gtaaacacgg tcgaggaagg cttcgttgtt
      541 tttgttatta cggaaagtga cccattcgga cttgttcgag tgggcgagaa taatcccgtt
      601 gaacggcagg gcggagatac cttccgtccc gttgtagtta ccttcctgag tggcggttaa
      661 caagggatgc agcactttta tcggtgcttt aaacatctca acgaattcca tgatcccctg
      721 attggcgcgg cacagcgcac cggaatagcc gtaggcgtcc gggtcattct gcgcgtagtg
      781 ttcgagttta cgaatatcga ctttcccaac cagcgcggag atgtcctggt tgttctcatc
      841 accgggttcc gttttggcga tagcaatttg ttgcagaatt gacggccaga ccttcactac
      901 ccggaactta gtgatatcgc caccaaattc atgcaggcgt tttgccgccc atggcgacat
      961 gatggtgccg agataacggc gaggaatgcc atactctttt tccagaatct gagcatcttc
     1021 ctgcggattg aaaagacaga acggatgatc gttgaccggg ctacgctcac cgttggcgct
     1081 caatacataa atcggtacga gctgcattaa tgatttcagt cgctcagcaa gcgatgattt
     1141 acccccaccc acaggcccca gcagatacag gatttgtttc ttctcttcca gcccctgagc
     1201 cgcgtgtttc agataagaga caatctgttc aatcgcgtct tccatgccgt aaaacacttc
     1261 aaatgccgga taacgtgcaa tgacccggtt agaaaagagt cgagaaagtc tggggtcctg
     1321 ggctgtatcg accatgacag gctcaccgat agccatcaat agccgctcag ccgcgttggc
     1381 ataagcactg cgatcttgcc gacaagtggt aagaaactcc tgcagtgtga actcttcgtc
     1441 cttggcagct tcatatcgct ggcgatagtg atcgaatata ttcatggtat gccgtccttt
     1501 cgttttttag cacaggttaa gagccgttca tatgaatagt agaggctccc ggaagaggta
     1561 aactgaacga cgtctcttgc taaaacacag caactctcat gccattttat cgacgatgag
     1621 aacaacgtgc ccggtgatac accttcaaaa ttaagcgtag atggcaaatg gaaaacttgc
     1681 ctgcacggct ttcttaattt caatggcata tcaacaactc atccataaat tttgcataat
     1741 taatgtaaag accaggctcg ccagtaacgc taaatccatt tggctgtaag cgcggtgtca
     1801 tccgcggcag gaaaattaaa cagttacttt aaaaaatgaa aacgtaaaaa ggttgggttt
     1861 cgatgtattg acgggtaaac tttgtcgccc gctaaacatt tgtttaagga atgattaatt
     1921 gtgaccaaac tcaaacttct ggcacttgga gtgcttatcg caacgtctgc aggcgtagcg
     1981 cacgctgaag gtaaattttc cctgggcgca ggcgtaggtg tcgttgagca cccatataaa
     2041 gattacgata ccgatgttta cccagtaccg gtaatcaact atgaaggcga taacttctgg
     2101 ttccgtggct taggtggtgg ttactacctg tggaatgacg caacggataa actttcaatt
     2161 accgcttact ggtcgccgct ttacttcaaa gcgaaagaca gtggcgatca ccaaatgcgt
     2221 cacctggatg accgtaagag caccatgatg gctggtctgt cttatgctca ctttacccag
     2281 tacggttacc tgcgtaccac cctggctggc gataccctgg ataacagcaa cggcattgtc
     2341 tgggatatgg cctggttgta tcgttacacc aacggtggcc tgaccgtgac tccgggtatt
     2401 ggtgtgcagt ggaacagcga aaaccagaac gaatactatt atggcgtatc gcgcaaagag
     2461 tccgctcgca gcggtctgcg tggctataac ccgaatgaca gctggagccc ttaccttgag
     2521 ctgagcgcca gctacaactt cctcggcgac tggagtgttt acggtaccgc gcgctacacc
     2581 cgtctgtctg atgaagttac tgacagcccg atggtggata aatcctggac tggcctgatt
     2641 tctaccggga tcacctacaa attctgataa tgcatctttc cagggcattt tgtgcataaa
     2701 aacagggcgt cacgccctgt tttgcattac ggtggtgcaa tacctgggag gaaacaatgc
     2761 aacaaaaaat gattcaattt agtggcgatg tctcactgcc agccataggg cagggaacat
     2821 ggtatatggg cgaagatgcc agtcagcgca aaacagaagt tgctgcacta cgcgcaggca
     2881 ttgaactcgg tttaaccctc attgataccg ccgaaatgta tgccgatggc ggtgccgaaa
     2941 aggtggttgg ggaagcatta accggtctgc gagagaaggt ctttctcgtc tctaaagtct
     3001 atccgtggaa tgctggcggg caaaaagcga taaatgcatg cgaagccagt ttacgccgtc
     3061 tcaatactga ctatctcgat ctctacttat tacactggtc tggcagtttc gcttttgaag
     3121 agactgttgc tgcgatggaa aaattgatcg cccagggaaa aatccgtcgc tggggcgttt
     3181 ctaaccttgg ttatgccgat atgcaggaac tctggcagct gccgggggga aatcagtgtg
     3241 ccactaatca ggtgctttac catctcggtt cacgagggat tgagtacgat ctactcccct
     3301 ggtgccagca acagcagatg ccggtgatgg cttacagtcc attagcccag gccgggcggt
     3361 tgcgcaatgg actgttaaaa aacgcggtag tcaacgaaat tgcacatgtc caacaacatg
     3421 cagctgcgct tgaggtcgaa ctttcgtcag cggaattagc tatgctggat aaggcatatc
     3481 cggcaccaaa aggaaaaact gcgctggata tggtgtgatg ttgatacgtg ccggatgcat
     3541 cgcacccggc acacaacatt aacgcaaatt aacgtttggc aacgcgaatg gattgcgcca
     3601 gatgtgcagg tttctctttg gtgacttttt gcgtttctga agcgtaagcc gtttctacac
     3661 aaacaaaggt cttgtagcca tcgtccggca tgtcgcccat gctaactgaa agcgccggtc
     3721 ccgggttcca gccgacaacg ttcagatgat gctggtggcc tacggcgata atacgattca
     3781 gcgcttcatc attaatcacg ctgcaatctt gcggattcag atacacgcga tcggtacggt
     3841 cagggaaggt ctgaataccg tcggtcagta catcttcttt cgcgtcattc actttatcaa
     3901 tgaagcgatc gcccagccca ctgacgctta ccttagcgat atcacccacg ttaaagtagg
     3961 tatgcagggc agaggtggtt tcaaattcgc catgtgattc aagatcgatt tcacaggttt
     4021 tacccacgcg gaaatgcgct aacagcgtaa agtcgtgcgg ccagaatttt ttcgtctctt
     4081 cgctttgtgt caattcaaaa gtcagcgcta cgccatcagc atcttcatga tgtgatttca
     4141 gcgtccacgg caggttgcgg gcaaaaccgt gcgcaggcag accttgttgt gccgccggac
     4201 caaaccacgg ccagcaaacc ggtacgccac cgcgaatagc gacgccattt ttgaacggtg
     4261 tgttgttgct caaccacaga acttcttctt cacccgcagg tttccacgag agaaggtgtg
     4321 cgccctgtaa tgcaaaagag gcttttacct ggggatgatc gaccacaatg aggtccagtt
     4381 catccagttt acgacgggag aggacagggg agatttgttc gatgaccgga agggcaaaaa
     4441 ttttcttaat catgacgcag tcctttaact tcattttatc aggtaaaaaa aagagcgacc
     4501 gaagtcgctc tttttagatc acagtgtcat ctcaacttat ttggagatgt gagcgatcag
     4561 gtccagaact ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa
     4621 gttgtcgttc agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt
     4681 gaaatcggta gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc
     4741 agcagcagct ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac
     4801 ggtcaggtca actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt
     4861 gccattcagt tctggcagta ctttacctac agctttagca gcaccggtag aggacgggat
     4921 gatgttctgg gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt
     4981 tttctgagta gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt
     5041 atcgttgata actttagcca gcggagccag gcagttggtg gtgcaggaag cgttggaaac
     5101 gatgtcctgg ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc
     5161 tttagacgga ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt
     5221 ttcgtcagtc aggaacagac cagttgcttc agcgacaacg tcaacaccca cttcgtccca
     5281 tttcaggtta gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag
     5341 atgaccgtct ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag
     5401 catgtatgcc atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga
     5461 acgtttctga gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac
     5521 tttgatagtc atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt
     5581 acaaaaacct tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg
     5641 tgcctaagca ttacgcgact gactcgcctc acttttcctt tgggcttgag accacatggg
     5701 gtcggcgccc cgaattttaa agggcaatca cgataaaaat gtgattttca tcacgattta
     5761 ggtgcagcca tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt
     5821 tttgttagaa tcagtcacgt aatgtgagca cgattaaagt gagatgtgag taaatggcga
     5881 ataaaccttc ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc
     5941 agaatcatgg gacagaaccg ccatttacgg gtcgtttact gcataacaag cgtgacggcg
     6001 tatatcactg tttgatctgc gatgccccgc tgtttcattc cgaaaccaag tatgattccg
     6061 gctgtggctg gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag
     6121 acttgtcaca tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg
     6181 ggcatgtctt tcccgacggg ccgcaaccaa caggcgaacg ttattgtgtt aactctgctt
     6241 ctttacgctt cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag
     6301 caacttattc cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac
     6361 gcctgaggta taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ctgatggcgt
     6421 tgcgttgacc gaggaacaaa aagagaattg cctgcaactg gtgatgctgt ggcaagcccg
     6481 ccataatacc gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag
     6541 caaacagcag ttaaaagaag attttggtat ctcagcaaag cctattgcca tgtttaagta
     6601 agtggttcat cccctttttg ccgcggctat gaaggggata gccaacaatg accccacgac
     6661 tcgtctcaat tatctcgacc cgacctggct aagtcccagt ccgatctctt gcagtcgcgt
     6721 tcacgcctga atgacttctc ctgaacttaa gtctcagacc tatttggccg gtaatccctc
     6781 tcgaatttgc tctttttgcg tcccgccttc caccaacctg taacagaccc agccattttc
     6841 tgcgtaattg tgatctgagt tgtaaaatgc aggtcattat ctgtgttgtg cagatttaat
     6901 attgacctca aaaatcatgt gggtaagtat gatggccatc taagcaaaac aaaacacaga
     6961 ggatgacgca atgaaagcac tggctcggtt tggcaaggcc tttggcggct acaagatgat
     7021 tcatgtccca caacccattt gtggcccgga agacgtagtg attgaaatta aagccgcggc
     7081 aatctgcggc gcagacatga agcactacaa tgtcgatagc ggttctgatg agtttaactc
     7141 tatccgcggc catgagttcg caggttgtat tgcgcaggtt ggtgaaaaag tcaaagactg
     7201 gaaagtgggg caacgcgtcg tgtcggataa cagcggtcac gtttgcggcg tttgtccggc
     7261 ctgtgaacag ggtgattttc tgtgttgtac agaaaaagta aaccttggtc tggataacaa
     7321 tacctggggc ggtggttttt ccaaatattg tctggttcct ggtgaaattc tcaaaattca
     7381 tcgtcatgcg ttgtgggaaa tccctgatgg tgttgattat gaggacgcag ccgtacttga
     7441 ccctatctgt aatgcctata aatccatcgc gcagcaatcg aaattccttc ctggtcagga
     7501 tgttgtcgtc attggcactg gcccactcgg gctgttttcc gtacaaatgg cgcggattat
     7561 gggggcggta aatatcgtcg tcgttggtct gcaagaagat gtggcggtcc gcttcccggt
     7621 tgcaaaagaa ctgggggcga cggcagtagt aaatggttct accgaagatg tggtggcgcg
     7681 ctgccagcaa atttgtggca aagataatct gggactggtg attgaatgct ccggtgccaa
     7741 tatcgcactg aaacaagcca tcgaaatgct ccgcccgaat ggggaagtgg tacgcgttgg
     7801 aatgggcttc aaacctcttg atttctcgat taatgacatt accgcctgga ataaaagcat
     7861 cattgggcat atggcctatg actccacctc atggcgtaac gctatccggc tattagccag
     7921 cggcgctatc aaagtcaaac cgatgatcac gcatcgtatc ggcctgtcgc aatggcgcga
     7981 agggtttgat gcgatggtcg ataaaaccgc aatcaaagtg atcatgactt acgactttga
     8041 tgaataacta ctctcctgaa ggaacgttat cgcatggaac agataacaaa accgcattgt
     8101 ggtgcacggc tggatcgctt accggattgc cgctggcatt cgtcaatgtt tgccatcgtc
     8161 gcgtttggct tgcttgtctg ctggagtaat gccgttggtg gcttgatcct cgcgcagctg
     8221 aaagcgttgg gctggacaga taattccact acagccacat tctcagcaat cacgaccgcc
     8281 ggaatgtttc tcggtgcgct ggtgggcggc atcattggcg acaaaaccgg tcgcagaaat
     8341 gcgttcatcc tctatgaggc cattcatatt gcctcgatgg ttgtcggtgc tttctcaccg
     8401 aatatggatt ttcttattgc ctgccgtttt gtgatggacg ttgggctggg agctttactg
     8461 gttacactgt ttgctggttt caccgaatat atgcccggta gaaatcgcgg aacgtggtcc
     8521 agtcgggttt cctttattag caactggtca tatccgctct gctcactgat tgcgatggga
     8581 ctcacgccgc tgattagtgc agagtggaac tggcgggtac aactgcttat tcctgcaata
     8641 ttgtcgctta tcgctacggc gctggcctgg cgctactttc ctgaatcccc gcgctggctg
     8701 gaatcgcgcg gacggtatca ggaagccgag aaagtcatgc ggagtataga agaaggcgtc
     8761 atacgccaga cgggtaaacc tttgccgcct gtggttattg ctgatgacgg taaagcgcca
     8821 caagcggtgc cgtattcagc cttactgaca ggagtattac tgaaacgcgt gatattaggc
     8881 tcttgtgtgc tgattaccat gaacgttgtg cagtacacac taattaactg gttgccaaca
     8941 atattcatga cccaggggat taatttaaaa gactcgattg ttttaaatac catgagtatg
     9001 tttggcgcgc catttggtat ttttattgcc atgctggtga tggataaaat tccgcgtaaa
     9061 acaatgggtg tggggctatt aatcctgatt gcggtgctcg gatatatcta ttcactgcaa
     9121 accagtatgt tgctcataac gctgattggt ttcttcctga ttactttcgt ctatatgtac
     9181 gtttgctatg cctcggcagt gtatgtccct gaaatctggc cgacagaggc caaactccgt
     9241 ggctccggtc tggcgaatgc ggtagggcga atcagtggta ttgccgcacc ttatgccgtt
     9301 gcagtgctgc tcagtagtta tggtgtaacg ggagtcttta ttcttctggg ggctgtttca
     9361 aatattgtcg caattgctat cgccaccatt ggaattgaaa ccaaaggtgt ctccgttgaa
     9421 agtttaagta ttgatgcagt agccaataaa taatacgggt ggattcaaaa tgaaaaattc
     9481 aaaagcaata ttgcaggtgc cgggcacaat gaaaattatt tcagcagaaa taccagtgcc
     9541 taaagaagat gaagttttga ttaaagtaga atatgtcggt atttgtggtt cagatgtaca
     9601 tggttttgaa tcaggcccgt ttattccgcc taaagaccca aatcaagaaa ttggcctggg
     9661 tcatgaatgc gccgggacgg ttgtggctgt gggaagccgc gtgcgcaaat ttaaaccggg
     9721 ggatcgggta aatatcgaac ctggcgttcc ttgcggtcac tgtcgttact gtctggaagg
     9781 caaatataac atctgcccgg acgttgattt tatggcgaca caacccaact accgcggcgc
     9841 attaacgcac tatctgtgtc atccggagag ctttacttac aaactgcccg acaatatgga
     9901 cacgatggaa ggggcgctgg tggagcctgc cgcagtcggg atgcatgccg cgatgctggc
     9961 agatgttaaa ccgggtaaga agataattat tctgggagca ggttgtattg gtttgatgac
    10021 gttgcaagcg tgcaaatgcc tgggcgcaac ggaaattgcc gtcgttgatg tactggaaaa
    10081 acgtctggca atgggggaac agcctggtgc gacagtggtt attaatggcg caaaagagga
    10141 cactattgca cgctgtcagc aatttaccga agacatgggc gcagatattg ttttcgaaac
    10201 agcgggttct gcggtcaccg ttaaacaggc acctttaatc tggtaatgcg cggcggtaaa
    10261 attatgattg ttggtactgt acccggcgat tcggcaatca atttcctcaa aatcaatcgc
    10321 gaagtcacta tccagacggt attccgctat gccaatcgtt atccggtcac gattgaagct
    10381 atttcttcag ggcgattcga tgtgaaatcg atggtgacgc atatttacga ttatcgggat
    10441 gtacaacagg catttgaaga gtcagttaac aacaaacgcg acattattaa aggcgttatt
    10501 aaaattagcg attaattaat acgggagaac agaacatgct cgcagatatc agatattggg
    10561 aaaacgatgc cacaaataaa tattatgcaa ttgcccattt taacgtatgg aatgcagaaa
    10621 tgttgatggg cgttaaagac gcagccgaag aagcgaaatc cccggttatt atttctttta
    10681 gtacaggttt tgtcggtaac acctcatttg aagatttctc tcacatgatg gtatcaatgg
    10741 cacaaaaagc aacggtgccg gtaataactc attgggatca tggtcggagt attgagatta
    10801 ttcataacgc ctggactcat ggtatgaatt cattaatgcg tgatgcttct gcatttgatt
    10861 tcgaagaaaa tattcgttta accaaagagg ctgttgattt ctttcatccg ctgggtattc
    10921 cggtagaggc ggaattaggg catgtcggta atgaaaccgt ttatgaggaa gcgctggcgg
    10981 gctatcacta taccgatcct gaccaggcgg ctgaatttgt tgaacgtacg ggctgtgact
    11041 cattggctgt cgccatcggc aaccagcatg gggtttatac gtcagagccg caattgaact
    11101 ttgaggtcgt caaacgcgta cgcgatgctg tttctgttcc gctggttttg catggcgcat
    11161 cggggatcag tgatgccgac attaaaactg caatttcgtt gggtattgcg aaaatcaaca
    11221 ttcatacgga gctctgtcag gccgcaatgg tagcagtgaa agaaaaccag gatcagcctt
    11281 tcctgcattt agaacgtgaa gtacgtaaag ctgtaaaaga acgggcattg gaaaaaattg
    11341 aactgttcgg ttctgatggc aaagcggaat aatgatatgg ataatctcga cgttatttgt
    11401 ataggtgccg ctattgttga tattccatta caaccggtca gtaaaaatat ctttgatgta
    11461 gattcttatc ctcttgaaag aatcgcaatg agcaccgggg gcgatgcaat aaatgaagca
    11521 acaattattt ctcgtctggg gcatcgtacc gcattaatga gccgcattgg taaagatgcc
    11581 tcagggcaat ttattctcga ccattgtcgc aaagagaata ttgatattca aagcctgaaa
    11641 caggacgtca gcatagatac atctattaac gttggactgg tgacggagga tggcgagcgg
    11701 acgtttgtca cgaaccgtaa cggcagtttg tggaaattaa atattgacga tgttgatttt
    11761 gcgcggtttc ctcaggcgaa attattatca ctggccagta ttttcaacag tcctctattg
    11821 gatggtaaag cgctaacaga aatttttacg caagcgaaag cccggcagat gatcatttgt
    11881 gccgatatga tcaaaccgcg gttgaatgaa acgctggatg atatttgcga agcattgagt
    11941 tatgtcgatt atctgtttcc aaattttgcc gaggaaaaat tactcacagg gaaagagaca
    12001 ctggatgaaa ttgctgactg ctttcttgcg tgcggcgtaa aaacggtggt gattaaaacg
    12061 ggtaaagacg gctgctttat caagcgtggt gacatgacga tgaaggtgcc ggcggtcgca
    12121 ggaataaccg ccatcgacac cattggcgcg ggcgataact ttgcttcagg ttttattgcg
    12181 gcactgttag aaggcaaaaa tctgcgtgaa tgcgcacgct ttgccaatgc aacggcggct
    12241 atctcggttc taagcgtcgg tgccaccacc ggcgtaaaaa acagaaagct ggtcgaacaa
    12301 ttgctggaag aatacgaagg ataatgaagg caaatgaaaa agataccttt aggcacgacg
    12361 gatataacgc tttcgcgaat ggggttgggg acatgggcca ttggcggcgg tcctgcatgg
    12421 aatggcgatc tcgatcggca aatatgtatt gatacgattc ttgaagccca tcgttgtggc
    12481 attaatctga ttgatactgc gctaggatat aactttggca atagtgaagt tatcgtcggt
    12541 caggcgttaa aaaaactgcc ccgtgaacag gttgtagtag aaaccaaatg cggcattgtc
    12601 tgggaacgaa aaggaagttt attcaacaaa gttggcgatc ggcagttgta taaaaacctt
    12661 tccccggaat ctatccgcga agaggtagaa gccagcttgc aacgtctggg tattgattac
    12721 atcgatatct atatgacgca ctggcagtcg gtgccgccat tttttacgcc gatagctgaa
    12781 actgtcgcag tgcttaatga gttaaaggct gaagggaaaa ttcgcgctat aggcgctgct
    12841 aacgtcgatg ctgaccatat ccgcgagtat ctgcaatatg gtgaactgga tattattcag
    12901 gcgaaataca gtattctcga ccgggcaatg gaaaacgaac tgctgccgct atgtcgtgat
    12961 aatggcattg tggttcaggt ttattccccg ctagagcagg gattgttgac cggcaccatc
    13021 actcgtgatt acgttccggg cggcgctcgg gcaaataaag tctggttcca gcgtgaaaac
    13081 atgctgaaag tgattgatat gcttgaacag tggcagccac tttgtgctcg ttatcagtgc
    13141 acaattccca ctctggcact ggcgtggata ttaaaacaga gtgatttaat ctccattctt
    13201 agtggggcta ctgcaccgga acaggtacgc gaaaatgtcg cggcactgaa tatcaactta
    13261 tcggatgcag acgcaacatt gatgagggaa atggcagagg ccctggagcg ttaaatactc
    13321 aacacgattc acctctgttt ctgtgaaaac tgtgatgaaa atctgtgatt caaataggtt
    13381 attttgaaag taaacatcgg ttatgcgata atcgcgctaa tgtgatgtaa aaagatacag
    13441 gggtgtaatc gtggcggcaa aagacaggat tcaggcaatt aagcaaatgg ttgccaacga
    13501 taaaaaggtg acagtctcaa atttgagtgg gatttttcag gtaaccgaag aaaccattcg
    13561 ccgcgatctt gagaagctgg aagatgaagg ctttttgacc agaacctatg gtggtgctgt
    13621 tttaaataca gcggtgttga cggagaatat ccatttttat aagcgcgctt catcgtttta
    13681 tgaagagaag ctgcttattg cacgcaaggc actacccttt atcgacaata aaaccaccat
    13741 ggcagccgat tccagtagta ccgtaatgga attgctcaaa ttgttacagg accgtagtga
    13801 cctgacgttg ctaaccaact cggcggaagc gattcatgtt ctggctcagt cagaaattaa
    13861 agtcgtttca acgggtgggg aactaaacaa aaatacactt tccctgcaag gaagaattac
    13921 taaagagatc atcagccgct accatgtcga tatcatggta atgagttgta aaggtcttga
    13981 tattaacagc ggcgcgctgg actctaatga agcggaagct gaaatcaaaa agacaatgat
    14041 ccgtcaggcg acagaagttg cgttattggt tgatcactct aagtttgatc gtaaagcttt
    14101 tgtccagtta gctgacttta gtcatattaa ttatataata actgataaat caccgggtgc
    14161 agagtggata gcattttgca aagacaataa tatccagctg gtgtggtaat ttaagcacac
    14221 gttgtcaata ttatttgttc ctgcgactgg aaataaaatg atgataaaga tgcggcaagt
    14281 gaatttgccg caattgctac cttttatagc aaatccgagg cacccaatgg aacaatatga
    14341 tcaaattggc gcaagactgg accgcttgcc tttggcccgg tttcattatc gtatatttgg
    14401 tataataagc tttagtctgt tattaacggg gtttttgagt tactcaggta atgtcgtctt
    14461 agcaaagctg gtaagcaatg gatggtcaaa taatttcctc tatgccgcct ttacctcggc
    14521 attaatgttt ggttatttca tcggctcact tactggtggg tttattggtg actactttgg
    14581 gcggcgcagg gcgtttcgca taaatcttct catcgtcggt attgctgcaa caggggccgc
    14641 ttttgtccct gatatgtact ggctcatttt ctttcgcttc ctgatgggaa caggaatggg
    14701 ggcgctgatt atggttggct atgcctcatt tacggagttt atccccgcga cggtgcgtgg
    14761 aaaatggtcc gcgcggctct catttgttgg taactggtcg cccatgctgt ctgcggcgat
    14821 aggcgtggtg gttatcgctt tttttagttg gcgaataatg tttcttctgg gtggtattgg
    14881 catactgtta gcctggtttc tctcaggtaa atactttatc gagtcgccac gatggctgga
    14941 agggaaaggg caaatcgccg gtgcagaaag ccaacttcgt gaagtagagc agcaaattga
    15001 aagggagaag agtattcgtt taccccagct tactttgaac cagagcaaca gcaaggttaa
    15061 ggtaatcaag ggtaccttca ggctcctgtt taaaggggaa atgttacgac gtacattagt
    15121 tgcgataact gttttaattg caatgaacat ttcgctttat accattaccg tatggatacc
    15181 gaccatattt gttaactccg gcattgatgt cgataaatca atattaatga ccgctgttat
    15241 tatgattggt gctccggtag gaatatttat tgcggcatta attattgatc attttcctcg
    15301 tcgattattt ggctccgcct tacttattat tattgccgtg ttaggctata tctattcaag
    15361 tcagactaca gagtgggcga ttttaatcta tggtctggtg atgatcttct ttttatacat
    15421 gtatgtttgc ttcgcgtcgg cggtttatat cccggagctt tggccaacgc atttacgcct
    15481 gcgcggttcg ggtttcgtta atgccgtcgg acggatcgtc gcagttttca cgccctatgg
    15541 cgttgcggca ttattaacac attatgggtc gatcacggtg tttatggtgc ttggtgtcat
    15601 gttagtgctc tgtgcgctgg ttctctccat ttttggcatc gaaacgcgga aggtgtcgct
    15661 ggaagagatt tctgaggtga attaaatctt tcgttgatgg catcaattgc cagatgtacc
    15721 actgacgtgt tttatttggc ctacaatgcc agtacagaat tgtaggccag cgtaaaatta
    15781 cccctgcgtc tcttcccagt ctgccagcgt atatagcgtt gccccagctg ctgacatctc
    15841 cataaacgcg tgtgcactgt cctggggctg gatattcacg ccacgacagc catcggtaat
    15901 cacgtttacc ttataaccta actgtaacgc gtccagcacg gtaaacttca cgcaatagtc
    15961 agtagccagg cccataacga tcaattcatc gatttcatga tcgcgcaacc agtcatcaag
    16021 cgcggttttc tgccgacgtc cgttatcaaa aaaggcactg taactgtcaa ctaaaggatt
    16081 ttcgccttta tggaacaccg ctgcgatctc tttttgattc agtaacggat gtaattgtgc
    16141 gccttcactg ttctgcacac agtgttcagg ccagaaagtt tgccgcaggc cgccgagttg
    16201 gcctggcgta taaggctcta cgccgtgctg gctggcaaag ctgccgtgat tcgccggatg
    16261 ccagtcctgg ctggcgataa ccgcttctcc gcgcgactgg caccagtcaa taaggcggtt
    16321 agcaacctcc accgtactgt caccttccgg tacggcgagc gcgccaccag cacagaaatc
    16381 attttgtaaa tcgaccagta acagggcgcg atggggcatt gcagtctcct taatcatccg
    16441 gcgtcagttc gccgcgcagg ttttggctca tggccttgcg aatggtttca gtatccagtt
    16501 cctggctcag caggtaatgc agtttggtta gcgtggcttc tacagtcata tctgcaccgt
    16561 caataacgcc ggcatgggcg agggcgttac cggtggcata accactcata ttcactttac
    16621 cggacataca ttgtgtcagg ttgaccacca caataccgcg atcgctggct tcttgtaatt
    16681 cctgcaggaa ggctttgttt tgtggcgcat tacccacgcc ataggagcgc agaatcaatg
    16741 ctttcaccgg ttggcgcaga aaattgcgca cgacgtcagc agaaatccct ggataaatcg
    16801 tcaccacgcc aattggttgt ggcgtgattg gatgaacgat taattcccct tcaccgtgcg
    16861 gggcgggtgg cgtattcaaa cgacgaatat ggataccagc ttccagtaac ggaggaaggt
    16921 ttggagaggc aaacgcatca aaaccatcgg catgggcttt ggtagtgcgg ttgccgcgat
    16981 acaatcggtt attgaaaaag agcgttactt cgttgatcgg ataattcgcc gcaacgtaca
    17041 acgcattcag cagattaatt tgtccgtcag agcgtaactc agccagcggg atttgtgacc
    17101 ctgtcacaat gaccggttta ccgagattct cgagcatgaa cgacagcgca gaggcggtat
    17161 acgccatcgt gtcggtaccg tgtagaatga caaaaccatc atagtcgtca tagtgcgctt
    17221 taatatcttc agcaatatgc tgccagtctt ccggcgtcat atcagaagaa tccatcaacg
    17281 gcgtatattc atgaatggtg aaatctggca tctccgggcg atggaattcc ggcatcagcg
    17341 ccagttggcg ttgtagatga cctgacaccg gaatataacc ctgctcggaa cgctgcatcc
    17401 cgatggtccc gcccgtgtag gcgacgtaaa ttgatttctt ttgcatgata ttgatatgaa
    17461 tgatatcgaa agagcaaaag tatatacgtg aggcaggagg agcgcacctg atactatgcg
    17521 gataaggaaa tatggggcga gacagcggga agaggattcg tcgaggtaaa aaataatgtg
    17581 gccgacgatc tgtcggccac tcagtacaag acttaacgca cgttggcgca ggtcaggcaa
    17641 aacgcataac ggttttgtgg gtcgttaaac gcggccagct tgtcgctttc acttttaacg
    17701 gtagaggcta ccgaggcaag cggtgcaggt aacatggcct ggaacgcatc tggcaacatt
    17761 gcccggacag aaccagacat gttgtccatc actttgtcga agaaggtcgg ttcatcaacg
    17821 tagtattcca gatgccactg tttcactttt gcgagctctg ctgctttggc aaccgcatca
    17881 tcgaaatccc cgagactatc gaccagcccg ttagcttttg catcctgacc ggtccagacg
    17941 tggccctggg cgattttatc aatttgctcc ggcgtcgaat gacgcgcatc agcaaccagc
    18001 gtgataaagc gtttatagcc attctcaatg cttaattgca tcatctgctg cgcttccggc
    18061 ggcagtgccc tggtgataga aacatccgcc agcggtgaag ttgagacacc atcagtatga
    18121 acaccaatcg aatccagact attttctacg gtggtgatca cgccgaagat accgatagaa
    18181 ccggtcaggg tgctggggtt agccacaatg taattagctg gcgtggaaat ccagtaacca
    18241 ccagatgccg ccatgccgcc catcgataca accacagact tacccgctgc ccgggctgct
    18301 gccagttcag cgcgaatcac ttcagacgcg gtaacgctgc cgcctgggct attaacacgc
    18361 aggacaatcg ctttcacttt cgggtcaagg cgagcgtcgc ggatttgtgc cgcagtggta
    18421 tcaccgccaa cattcccctg agtttcctcg ccatccataa ttgcgccatt agcgaagacg
    18481 acaccgatgc tgtcaccggt atctgccggc gttttcaatg cgtaatcgta ataactgatg
    18541 gcgcgataat ttttatcagt cttactccag ccgaactctt tggtcagagc tttttcgatt
    18601 tcggcactcg atgccagtgc atcgaccagc ttgttttcca gtgcatattt cgcggtatcg
    18661 ccaccggttt tggttaaacc ctcaagcaac ccttgcgcgc cagggaatac ctgctgagca
    18721 gggatctgcc ggttagcggc aacagtattc agatagtttt gccacagctc accaatccag
    18781 cggctgtcag cttcgcgggc tgccggtgac atatcatcac gaataaacgg ttcaacggca
    18841 gatttatacg tacccacgcg gaacacatgg gtggaaactt tcagcttatc cagcaacgat
    18901 ttgtagtaca gaccgttggt agcaaaaccg tgcagatcaa ccacgccttg cggtgacagc
    18961 caaattttat tggcgaaact ggcgagataa tattgcccct ggctgtagtt ctcgccaacg
    19021 gcataaaccg gtttcccgct gtcacgaaac tctttcaggg ctttgccgat gtactgcata
    19081 gacggctggt cgccgcctgc gaagtttttc agatccatca cgataccggt gatattgcgg
    19141 tcgtccttcg cctggcgaat ggtgttgacg atatcaaaca gtgagttttc ctgcagacga
    19201 tcggaactgg caccaagcag ctggcggctt aatttactaa accgctgaga actgtctggt
    19261 ttatcgacga tcacaccaga aatgtccagc agcagtgcgc cacgactggc cgtttctttc
    19321 gaatcaccac cactgacctg catccagatc cccacaccaa ccagtacgag gaaaataaag
    19381 aacaggttaa gtaccatttc acggacgaaa ttcagcagac gccacgtcca tttaaaaaat
    19441 ccggcaataa atcgccaaag ggttcgcatg tattctccca acttaaggtc acacctgtca
    19501 caggcgcaat agatgtggtt atcgtaatga cccaaccggc acttgtcagc aggaattgcg
    19561 gactgtgctg taacaaaatc cactctcgtg ttaattttgt gaataaatat cacgacagga
    19621 gttaatcaaa tggatgcact cgaactattg atcaatcgcc gtagcgcctc ccgcctggct
    19681 gaacccgcgc caacgggtga acaactgcaa aacatcctgc gtgcgggtat gcgtgcgccg
    19741 gaccataagt ccatgcaacc gtggcatttt tttgtgattg aaggggaagg gtgcgagcgt
    19801 ttcagcgccg tactggaaca gggggcgatt gctgccggta gtgatgataa agctatcgac
    19861 aaagcccgta atgcgccgtt ccgcgcaccg ctcatcatca cggtggtggc gaaatgcaaa
    19921 gaaaatcata aagtcccgcg atgggaacag gaaatgtctg ccggatgcgc ggtcatggcg
    19981 atgcaaatgg cagccgttgc ccagggattt ggcggcatct ggcgcagtgg cgcattaact
    20041 gaaagtccgg tagtgcgtga agcattcggt tgccgtgagc aggataaaat tgtcggtttt
    20101 ctctacctcg gcacgccgca gttgaaagca tctacgtcga ttaacgtccc ggacccgacg
    20161 ccgtttgtaa cttatttctg ataattgtcg cgaaactgtc tggattgtga gcaaaggcgc
    20221 aggaattcag acactctcac ttatcacttc acggaatgag ggttaccata cccggattgc
    20281 attgacagga gatgtccatg agcgagaact cgattcgttt gacccaatac agccacggag
    20341 ctggttgcgg ctgtaaaatt tccccaaaag tgttggaaac catcctgcac agtgagcagg
    20401 cgaagtttgt tgatccgaat ttgcttgtgg gtaatgaaac ccgcgacgat gcggcggtgt
    20461 acgatctggg caatggcacc agcgttatca gtaccaccga cttctttatg ccgatcgttg
    20521 ataatccttt cgattttggc cgcattgcgg cgactaacgc catcagcgat atcttcgcga
    20581 tggggggcaa accgattatg gcgattgcga tcctcggctg gccgattaac aaactttccc
    20641 cggaaattgc ccgcgaagta accgaaggtg gacgctatgc atgccgtcag gcgggtattg
    20701 cgctggctgg cggtcactcc atcgatgcgc cggagccgat ttttggtctg gcggtaacgg
    20761 ggatcgtacc gaccgagcgg gtgaagaaaa acagtaccgc acaagccgga tgcaaactgt
    20821 tcctgacgaa accgctgggg atcggcgttc ttaccacggc tgagaaaaaa tcactgttga
    20881 aaccagaaca tcagggactg gcgacggaag tgatgtgccg gatgaacatc gcaggcgcgt
    20941 cctttgccaa catcgaaggc gtaaaagcga tgaccgacgt tacgggcttt ggtctgctgg
    21001 gccacttgag cgaaatgtgt cagggggctg gtgtgcaggc acgcgtcgac tatgaagcga
    21061 tcccgaaact ccccggtgtt gaagagtaca ttaagttggg cgcagtacct ggcggcactg
    21121 aacgtaactt tgccagctac ggtcatctga tgggtgaaat gccgcgtgaa gtgcgcgatc
    21181 tgctgtgcga tccgcaaact tctggcggtt tgctgctggc ggtcatgccg gaagcagaaa
    21241 atgaggttaa aactacagcc gccgagtttg gcattgaact gacggcgatt ggcgaactgg
    21301 tgccagcgcg cggcggtcgt gccatggttg agatccgtta atttaatgcg gttgtttatt
    21361 gccgaaaaac cgagtctggc gcgcgccatt gctgatgtcc tgcccaaacc gcaccggaaa
    21421 ggcgatggct ttatcgagtg cggtaatggt caggtggtga cttggtgtat cggtcacctg
    21481 cttgagcagg cgcagccaga cgcctacgac agccgctatg cgcgctggaa tcttgcggat
    21541 ttgccgattg tcccggaaaa gtggcaatta cagccacgac cctccgtgac caaacaactt
    21601 aacgtcatca aacgcttcct gcatgaagcc agcgaaatcg ttcacgccgg ggacccggat
    21661 cgtgaagggc aattgctggt ggatgaagtg ctggactatc tgcaactggc accggaaaag
    21721 cgccagcagg tgcagcgttg cttgataaac gacctgaacc cgcaggcggt tgagcgggcg
    21781 atcgaccgtc ttcgttccaa cagtgagttt gtgccattgt gcgtttctgc gctggcgcga
    21841 gcgcgtgccg actggctgta cggcatcaat atgacccgtg cgtataccat tctcggtcgc
    21901 aatgccggtt atcagggcgt actttccgtg ggacgcgtgc agacacccgt gctcgggctg
    21961 gtggtgcgcc gcgatgaaga gattgaaaac ttcgtggcga aagacttctt tgaagtcaaa
    22021 gcacatatcg tgacacctgc cgatgagcgg tttaccgcta tctggcaacc gagcgaagcg
    22081 tgtgaaccgt accaggatga agaagggcgc ttgttacatc gtccactggc ggagcatgtg
    22141 gttaaccgca ttagtggtca accggctatt gtcaccagct ataacgataa acgggaatca
    22201 gaatccgcgc cgctgccgtt ttcgctttcg gcgttgcaga ttgaagcggc aaaacgcttc
    22261 ggtctgagcg cgcagaacgt gcttgatatc tgccagaagc tgtacgaaac gcacaagcta
    22321 atcacttatc cgcgttccga ttgtcgctat ttgccagaag aacattttgc cggacgccac
    22381 gcggtgatga atgccatcag cgttcatgca ccggatctgt tgccgcagcc agtggtagat
    22441 ccagatatac gcaaccgctg ttgggatgac aaaaaggtcg atgcgcacca cgccatcatc
    22501 ccgaccgcac ggagttctgc gatcaacctg acggagaacg aagcgaaggt ctataacctg
    22561 attgcccgtc aatacctgat gcagttctgc ccggatgcgg tgttccgcaa gtgtgttatc
    22621 gaactggaca ttgccaaagg caaatttgtc gctaaagccc gttttcttgc tgaagcaggc
    22681 tggcgcacgc tgttaggcag caaagagcgc gatgaagaaa acgacggcac gccattgcct
    22741 gtggtggcga aaggcgatga gttgctgtgt gaaaaaggtg aagtggtaga gcggcaaacc
    22801 cagccgccgc gccattttac cgatgcaaca ctgctttcgg cgatgaccgg gatcgcgcgc
    22861 tttgtgcagg acaaagatct gaaaaaggtc cttcgtgcga ccgatggtct ggggacagaa
    22921 gcaacccgtg ccgggattat tgaactgctg ttcaagcgtg gttttctgac caaaaaaggg
    22981 cgctatatcc actccaccga cgccggaaaa gcgttattcc attcgctgcc agaaatggcg
    23041 acgcgaccgg acatgaccgc gcactgggaa tcggtgctga cgcaaatcag cgaaaagcag
    23101 tgtcgctatc aggactttat gcagccgctg gtggggacgc tatatcagct tattgatcaa
    23161 gccaaacgta cgccggtgcg gcagtttcgc ggcattgtgg ctccgggcag tggtggcagt
    23221 gctgataaga aaaaggctgc accgcgtaaa cgtagtgcga aaaaaagtcc gccagcagat
    23281 gaagccggaa gcggggcgat agcgtaagcg agcgaatctt tcgtgctatt cgagtcatat
    23341 tctgaaatat ccagcggatc aagaaaattc gttggatatt tttttgcatg gataaaatta
    23401 tcgcctctaa agtatgtaat aacagggaat gtgatgaaaa aagttttatt gcaaaaccat
    23461 cctgggagcg agaagtattc ttttaatggc tggggaatat ttaatagtaa ttttgaacgg
    23521 gtgattaaag aaaataaggc catgctgctt tgtaagtggg ggttttattt aacatgtgtt
    23581 gtcgctgtaa tgtttgtatt cgcagcgata acatccaacg gtttgaatga aagaggcctg
    23641 attaccgcgg gatgctcttt tctttatcta ttaattatga tggggcttat tgttcgggcc
    23701 ggttttaaag caaaaaaaga acaactgcat tattatcagg ctaaaggtat tgagccgctc
    23761 agtatcgaga agttacaggc gctacaattg atcgcacctt atcgattcta tcataagcaa
    23821 tggtctgaaa cgctagagtt ctggccgcga aagcctgaac ctggcaaaga taccttccaa
    23881 tatcatgtgc ttccttttga ctcgatcgat atcatcagta aaagacgcga gtctttagag
    23941 aatcaatggg gtatcgaaga tagcgaaagt tattgtgcct taatggagca ttttctttct
    24001 ggcgaccatg gagccaatac ctttaaagca aacatggagg aagccccaga gcaggttatc
    24061 gccttgttga ataaatttgc tgtttttccc tcagactata tctctgattg cgctaataat
    24121 cgctccggta aatcctcggc gaagctaata tgggcggcgg aattatcatg gatgatctcg
    24181 atatcaagca cagcttttca aaacgggaca attgaagaag aactggcctg gcattatata
    24241 atgcttgctt ctcgaaaggc gcacgagttg ttcgaaagcg aagaagatta tcaaaaaaat
    24301 agtctaatgg gatttcttta ctggcatatc tgctgctatc gcagaaagtt aacggatgct
    24361 gaacttgaag cgtgttatcg ttacgacaag cagttttggg agcactacag taagaaatgc
    24421 cgttggccca taagaaatgt tccctgggga gcatcatccg ttaaatactc ataaacgcct
    24481 gaaattttgc cgggggcgct gcgcttgacc ggcctacatt agagcgtaac tgcaatgaat
    24541 tgtgcccatc tgtaggcctg ataagcgtag cgccatcagg catttacaac ttaaatcaca
    24601 ccctgcgcca gcatcgcatc ggcaaccttc acaaaaccgg caatgttcgc gccctgcacg
    24661 tagttggttt gctcaccttc accaccatgc tcaacacagg catggtggat atccagcatg
    24721 atgtgatgca aacgtgcgtc aactttctcg gctttccagc ccaggcgcgc agcgttttgt
    24781 gccatttcca ggcccgacgt tgctacaccg ccagcattag ccgctttacc cggtgcaaac
    24841 agtacgcctg cctgctggaa cagttcagtc gcttcgatgg tggtcggcat atttgcccct
    24901 tcagcgacgg ctttaacgcc attggcgata agctgatgcg cggcgtcaac atccagttcg
    24961 ttctgggtgg cgcaaggcag ggcgatatca accggtacag accacggctg ttggccttcg
    25021 agatagacca gaccaaattc tttggcgtaa tctgccactc gaccatcgcg gctggctttg
    25081 atttcgataa gacgtgccag tttctctttc gtgaatccgc tttcatcaac tacagtgccg
    25141 ctggagtctg acgcagtgat cacacgagca ccaaattcca tcgctttttc gatagcgtac
    25201 tgggcgacgt tgccggagcc agaaacggaa acgctcatcc cttcaaaacc cataccgtgg
    25261 cgttttaaca ttgcttctgt gaaataaacc agaccgtagc cggtagcttg cgaataagac
    25321 taccgccaaa tgaaaggccc ttaccggtga agacgcaggc ggtattgttg gagagctttt
    25381 tcatcatccc cgccataaag ccgacttcac gaccaccaac gccgatatca cctgccggaa
    25441 cgtcggtatc cgcgcccagg tggcgataca gttcagtcat cagcgtctgg caaaaacgca
    25501 tcactttacc ttcgcttttt cctttcggat cgaaatcgct gccgccttta ccaccgccca
    25561 tcggcagggt agtcagggca tttttgaagg tttgttcaaa gccgaggaat ttgagaatgg
    25621 aaaggttaac tgacggatgg aagcgcatac cgcctttgta cgggccgatg gcagagctga
    25681 actgcacacg ccatgcacgg ttgacctgta tctggttgcg atcatcaacc cataccacgc
    25741 gaaactggat cacgcgctcc ggttcaacca gacgctccag taatgacatc tggcgatatt
    25801 ttggattttg ttcaagaaaa ggccagagtg tggtcattac ttcacgaacg gcttgcgcga
    25861 actcggtttg attcgggtcg cgcttttgga catggttgag gaatgactcc agagaatatg
    25921 tctgatccat agatataaaa accttatata ttaatacgat tgtgcttatg ttgtttatgt
    25981 catgtgcttt tgcagttttc gactatacca tcaagaatgt gacggtaagc aagaaaaaaa
    26041 tgacccagga aagcaaatta ataacgagag taatctcata accaaatgcg ataactcaga
    26101 cgctaaagtt ttgccacccg gctaccgtta tcagtagcag agacaataac gttaaggagt
    26161 tccctgtgag tcgagcattg ttcgccgtag tattagcatt tccattaatt gcactggcta
    26221 acccgcatta tcgtccggac gtggaggtaa acgtaccgcc agaagtgttc agttcaggcg
    26281 ggcagtcagc gcagccctgt actcagtgct gcgtttatca ggatcaaaat tattctgaag
    26341 gggcggtaat taaagccgaa ggaatactgc tgcaatgcca gcgtgatgac aaaaccctta
    26401 gcactaatcc gctgctctgg cggcgcgtaa agccataaac gcctctaata atggaatgtc
    26461 agcaggggcc agcggatatt gcagcgcctc ttcaggtgag caccagacca gcgcctgatg
    26521 ttcatgtgcc tgtaacgtcc cgtggaagtc gggtacgtgc caggcatgaa gatggataat
    26581 ccggcccgaa acttctcgct gatggctggc aacatactca cccacagttg cttcgatgtc
    26641 caattcttcg tttaactcac gcaccagcac ctgctgctgg ctttcatcca gctcgacttt
    26701 acctccggca aactcccata atcccgcctg atcgctctgg gcggggcgtt gcgcgagtaa
    26761 aattttgcca tcacgttcaa tgatggcggc aacaacttca atcattttca taaattacac
    26821 acgggcagat aagcgaaaaa tgccataata tccgcactgt tgctcaatca gaaaggcttt
    26881 atatttaagg agtgcctgtg ctagaccgcc atcttcatcc ccggattaaa ccgttgttgc
    26941 atcagtgcgt gcgggttctt gataaaccgg gcattacgcc agatggttta acattagtgg
    27001 gatttgccat cggcgtgctg gctctgccgt ttctggcgct gggctggtat ctggcggcgc
    27061 tagtcgtcat tttgttgaac aggctgcttg atggtctgga cggtgcgctg gcgcggcgca
    27121 gagggcttac cgacgcgggc ggctttcttg atatttctct cgattttctc ttttacgcgc
    27181 tggtgccgtt tggctttatt cttgctgcac cggagcaaaa tgcgctggca ggcggctggt
    27241 tgttgtttgc gtttatcggc acgggcagca gttttctcac ctttgctgca ctggcagcaa
    27301 aacatcagat tgataacccc ggttatgcac ataagtcgtt ttactatctg gggggattaa
    27361 ccgaaggcac agaaacgatc ttactgtttg tgctgggatg tttattccct gcatggtttg
    27421 cgtggtttgc gtggtttgcg tggatcttcg gtgcgttgtg ctggatgacg acctttacgc
    27481 gggtgtggag tgggtatctg acgctgaagt cgctccagcg tcagtaattg caatgttatt
    27541 tgctactgcc cgggccgcgt tcaccggttg caaccggatt ttttggatcg ctgctccatt
    27601 cgtaccagcc gccgtcatac acggaaacat tcttccaacc catggcgcgt gcgtacataa
    27661 aggtttcgga cgcgcgccag ccggtgccgc agtagaatga aacttgctga tctggtttga
    27721 tattccatgc tttccacata gcggtaatat catcggcgct acgcatggtg ccatccgggt
    27781 tatagaaatc ttccatatgc gtcgagtcgc taccagcgtg tccccaacgt gctccggcta
    27841 tttcaccttt tggtttaata tagctgtaac cgctggtcgt accgataaat tctggccacg
    27901 aacgaatgct caccagcgat gcatcctggc gatgcagcag tccacgggcc tgttccatat
    27961 cgagcatcaa ctgcggttgt gccgggatct tcacgccgaa atccggttcc gctttcactt
    28021 tcggtggcgt tcctcgctca acaggcagtc ccgcgtcgga ccaggtttgc cagccgccat
    28081 ccagcagacg cacatctttc acgcctgcgt agagcataat ttgcgccaca cgcgctccag
    28141 cgtatacgtc acgcccgtac agaatgaccg tggtgtcatg gcgaatgccg tgttttgcca
    28201 gcatcgcttt tagttgttca tcagaaactt tgttccacag cggttcgctt tccacttcgt
    28261 tggtatcgat gtagtcagcg ccaggaatat ggctgataag gtaaagctta ggagcgccct
    28321 aggccgcttc aatgactttc cagtcaccga caggtttcgc cgtaacctct ttaccttgtt
    28381 gcaggtcatg cagccattgc ggataaacca gctgctcaaa gtgcggcagt ttttgtagac
    28441 gggaaggttc gcttagcgcg tcactcagaa tggagatatg cgtaaaacct gctttttgca
    28501 gtcgcgtttt gacggcgtcg acatctttgc cattaccgta cagcgccacc ggggcatcgg
    28561 ctttcaggtt atgttgcttg atccacgcgt tgagctgttc ggtgctcatt ttgtcgagcc
    28621 agctagcaga gaggtttaag gcggcaggtt catgaccaga agggccattt aaggtttgtg
    28681 gccagccgtt ataaaacgcg ctggggcgag tatctatcgc tttgccattt tgttgctgaa
    28741 gttggtcaag tgtaagaggc ttcgccagtt cagcggccca cgaagaagcg caagccagcc
    28801 ctaaagccat tgccagcgcg gtcatttgag aaacacgttt catcgaaaat cccgacattg
    28861 tagttttgag tgcgccattc tgaactgacg cattgagaaa aactttgcgt tatcgcagtt
    28921 tgttgtaatt ttctgaccac tgcgccatat ccagaacaga actatcagca ggaacatcct
    28981 ggagatcgtg cgttacctga acgacgggga tcgccagggc gcgaacttcg ctgaacaccc
    29041 actggcgaaa attatcgcgc agagccacat caagacggct gaatggctca tccaggagta
    29101 acgcttttgg ctgggcgaga agggcgcgta gtagagcaac gcgcgctcgc tgaccgccag
    29161 acaaagtggc aggatcctga tggaaagctc cgtcaaggcc tgaacgctca agtgcatcat
    29221 tcacggcatt acgtcgggca ttccccttaa gtgtcgccgg tagcgccagc agtaaatttt
    29281 gcccgacgct gaactggtca aataacagtg catcctgaaa aagaatgcca atctgacgct
    29341 gtgcggtggg tagcatgtca atccgttgct cattgagcca tagctcacct gtacaagaaa
    29401 actgtccggc cagtgcacca atcatccatg aaaacagagt ggattttcca cagccagacg
    29461 gccccattaa cgtgacgatg tcacctttat caaccgtaaa gttaacgttt gtcagcaagc
    29521 ggctttctgg taaacgtagc gaaacatttt tcacgcagag cattagcgga ttccttgtct
    29581 gacataacct acccattttg cgactaacgc ggtcagggca aaaataataa gcggtaacaa
    29641 cagttgccat aacgcctggg cggcgagaat accgttgctg ccgccgctgc ttaatgccac
    29701 cgcctcagtg gtgagcgtcg gaaaacgccc cgcgcccagc cacagcgtcg gcatatactg
    29761 cgcaatactg actgaaaatc ccaccgcgaa ggcagtcagc acagggcgca acatgagtgg
    29821 gcatttcacg taaaagaaga ttttggcccg cgaccagccc agtgtttgcg caatcaaaat
    29881 taaccgtgaa tcaatgcgct gccaggcggg ttgcaggata aacagcatcc acggcattac
    29941 ccacagcaga tgcccccaga ccaccgccgt ccagcttcca tccaggttca gccatagcgc
    30001 cagcgtgtac tggcccgcca ccagtggcag agcgggcaat agaatgggca accatagcca
    30061 caactggcga cgctgtgccc cccattccag ccacagcagt aacaggagca aagcgatgaa
    30121 tgttgctacc agccccattg tcagactgtt gatgagtgct tcactattga tcgtcgactg
    30181 atccgcgagg atcgccagca gaaccacaca cagcacaccg gttaagggta aaaaactcgc
    30241 cagcgtagtg cctggtaata aaggtgtggc aggcttgcga atgccatcta cgcggggaat
    30301 agtgcgccgc cagctgcgcc aaaacgtagg cggcgagtaa cagcatcagc agcagactgg
    30361 cgagtgcgcc tttcgtttgt tgatccgcgt cgccctgagt taaccactgc cagctaatta
    30421 ctgccagcgt cggtggatta cctggcccaa gaataatcgc cacatccacg accgaaagcg
    30481 accaggcaac aatcgccagc atcgccattg ccagcgaagg cgcgacggag ggcaacagca
    30541 gccagttcag gcattgccag cggctgtagc ccagcgaatc cagcacaatg acctgctgca
    30601 acagccgttt ttcgctcaat actgccacta agatccacag cagaaatgcg ctttctttca
    30661 ctgcaagggt gaggcccaga ccgatccaaa tcggtccatt ggcggagtga aatacgggaa
    30721 atagtcatat agcagccctc cgtcagcaaa gagcagcagg gcgctggtgg caaaagccac
    30781 atggggaatg gcgagcagcc acggcagacg ggcgcacata cgctgccatt tcggcccagg
    30841 ccatagcgcc acaatcacca gcagggcaat caacaatgcc ccgaccgccg cgatggttgt
    30901 cgacaccagc gttgccagta atgcctgcgg taactgtgga tcggcaaata acgcctgcca
    30961 gcgtgtcaac gacaacgcag gcgatatcag cgtgagagct gccgggatca gcggtgcata
    31021 aatcaccgcc accatcgccc acagcaaaaa gattaatgcg taccgtaacg gcgtaaccat
    31081 tcttgttcca gtgcattcac ccatcctgcg tgcggttcag ccagtaccgg cggcagatcc
    31141 tgcggcattc ttgattgcaa tatttcgcgc tgcccgtcag gcagtttttg cggatcgaga
    31201 acagaaggat cgccccagac aacgggatct gctttacgca gttgcgcatc gggtgagagc
    31261 aggaaattgg caactacctt cgccgcagca ctggcattcg cgttggctgg aatggtgacg
    31321 aaatgcacgt tgccaatcat cccctcgcga aagccaaaac tgtaactgct tgccggcaaa
    31381 tcgccgctgg cgattttttg ctgcgcatgt gcggggttaa aggtcagcga caggcgcaat
    31441 gtgccggctt tcagcagagc atccatccgc gcgggcgaag gcgggaaatc ctttccttcg
    31501 cgccacaaat acggatgcag tgcatcaaga tattgctaca agggcgcagt gacacgggcg
    31561 aaagtcgcat cgtccggcgc ttcttttaat gcggcgggat ctggcgtcag cataatcagc
    31621 aactgttcaa gaaacgccgt gccggtaaag tccggtgggc gcggataagt aaccgtgccg
    31681 ggattggctt tagcaaactc cagtaaggct tgcggcgttt gtggtggctg tggcgtaaca
    31741 tcgcggcggg cgataaacgt cagttgtgcg ccgccccagg gcgattccgc accttgtgtc
    31801 ggcactgaaa aatctccccg caccggcagc tgtgtgtcga catagcgcca gttgggcaga
    31861 gtctccgccc agcccgtttg taataaattg gcctctttta aggt
//
LOCUS       DAOFMX010000047        31454 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.047, whole genome shotgun sequence.
ACCESSION   DAOFMX010000047 DAOFMX010000000
VERSION     DAOFMX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.047"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..357
                     /locus_tag="QBA20_002388"
     CDS             <1..357
                     /locus_tag="QBA20_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDQ6292891.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            707..1069
                     /locus_tag="QBA20_002389"
     CDS             707..1069
                     /locus_tag="QBA20_002389"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HDQ6292892.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            1066..1983
                     /locus_tag="QBA20_002390"
     CDS             1066..1983
                     /locus_tag="QBA20_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDQ6292893.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            1996..3261
                     /locus_tag="QBA20_002391"
     CDS             1996..3261
                     /locus_tag="QBA20_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HDQ6292894.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(3328..3951)
                     /locus_tag="QBA20_002392"
     CDS             complement(3328..3951)
                     /locus_tag="QBA20_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6292895.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(4699..5040)
                     /locus_tag="QBA20_002393"
                     /pseudo
     CDS             complement(4699..5040)
                     /locus_tag="QBA20_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head-binding protein"
     gene            5105..5563
                     /locus_tag="QBA20_002394"
     CDS             5105..5563
                     /locus_tag="QBA20_002394"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013043.1,HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDQ6292896.1"
                     /translation="MEVLDYNKETGIFTWKKKLSARGAVGKVAGTISYGYNAINIDGV
                     RYFAHRLAWLYVYGEWPKQEIDHIDRNRRNNAISNLRDVSRVVNALNVGPRNSNAGIK
                     GVTFCQARNQWQAQINVSGKNITLGRFNTIDEAAIAYKAANMVADHLLSR"
     gene            5695..5955
                     /locus_tag="QBA20_002395"
     CDS             5695..5955
                     /locus_tag="QBA20_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDQ6292897.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LAKQSSASVYIDDAERIADQQSDMVKKIVFDTLKELYKKDCN"
     gene            complement(5973..7814)
                     /locus_tag="QBA20_002396"
     CDS             complement(5973..7814)
                     /locus_tag="QBA20_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292898.1"
                     /translation="MVIGSLADAANQRGDFAENAAINAGLNIATHGLINGVTHGVRGA
                     SNIISGNKTSAQRATTAATETSPFSGTAPTETSPFSGDAAAATNPAVHAAEARAAQGV
                     PMTPATRDPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDSLLPSHFSG
                     NEQYKAVEQAIKSRAGSALRVQENEAIRQLAQGAGEIIDRVSGAKDALGMSDKFIDTI
                     NGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNLDPIEKRVF
                     RAVNPGKNGALTYANLNKQRRLVGDALHKNSGPYKDADRAALSRLYGSLADDQKAALS
                     ETNALRDFEVAQRLVQMRKSMEEQMINLTGRTLNGDVSRKATTALQAMSKGDAKGFRE
                     LMQNTPSRKLRTELLGTGLRDMLSNGKRGADFNPAGFADWYQNMLANGQMRNLARHLP
                     KETMSGLNDVYKVAKAIKDAKSYEITTGRLNEFVKRFNRVTAANEFVANHAQRIGTAV
                     GSTVSGPFSAVGAVAGSEIGAKVASKIRAMGGAASMESAEKLISSPEFQKAARLAVRQ
                     APESIVDTTVRRSSAWRSFYNSLPESDKKTISRLGIMYWMNSDDNQK"
     gene            complement(8234..9640)
                     /locus_tag="QBA20_002397"
     CDS             complement(8234..9640)
                     /locus_tag="QBA20_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HDQ6292899.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATEYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELVRVGVDPNNVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFVFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            complement(9650..10345)
                     /locus_tag="QBA20_002398"
     CDS             complement(9650..10345)
                     /locus_tag="QBA20_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HDQ6292900.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATGNQLATIAPTLGQQWLSGQMNNYQNLANIGLGALQGQANAG
                     QTYANNMSQISQQSAALAAANANKPSGLQTAISGGASGAMTGAALGSIVPGLGTGLGA
                     AIGGGLGLLGSLF"
     gene            complement(10348..10803)
                     /locus_tag="QBA20_002399"
     CDS             complement(10348..10803)
                     /locus_tag="QBA20_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HDQ6292901.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(10803..11651)
                     /locus_tag="QBA20_002400"
     CDS             complement(10803..11651)
                     /locus_tag="QBA20_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292902.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILVDHEIRLASAEAKIQDHETRITNAEAAIVSLD
                     SRLTTAENDIDYLTDEVVAIQNTLSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            complement(11651..13069)
                     /locus_tag="QBA20_002401"
     CDS             complement(11651..13069)
                     /locus_tag="QBA20_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HDQ6292903.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQSAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPADSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRIITKSPVTLSGAQIRIE"
     gene            complement(13069..13569)
                     /locus_tag="QBA20_002402"
     CDS             complement(13069..13569)
                     /locus_tag="QBA20_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HDQ6292904.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDVSPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            complement(13547..13801)
                     /locus_tag="QBA20_002403"
     CDS             complement(13547..13801)
                     /locus_tag="QBA20_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292905.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFAHPDDL
                     LKDVAEPDPDPEPEPEQEEKQRKKPGRKPKAAADEPDNEG"
     gene            complement(13846..15141)
                     /locus_tag="QBA20_002404"
     CDS             complement(13846..15141)
                     /locus_tag="QBA20_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HDQ6292906.1"
                     /translation="MALNEGQLVTYALDEIIETVQNLTPMASKVTKYTPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLSGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVTEDAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     TGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTTVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            complement(15141..16052)
                     /locus_tag="QBA20_002405"
     CDS             complement(15141..16052)
                     /locus_tag="QBA20_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HDQ6292907.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDNAGQEEGFEI
                     VLKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYDAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            complement(16066..18231)
                     /locus_tag="QBA20_002406"
     CDS             complement(16066..18231)
                     /locus_tag="QBA20_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDQ6292908.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPVVRKLVAEMRRNPIDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEKKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKAAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANSQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQDLLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLKLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            complement(18232..19731)
                     /locus_tag="QBA20_002407"
     CDS             complement(18232..19731)
                     /locus_tag="QBA20_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDQ6292909.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENGEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFEEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQAQVQLW
                     WDKDADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHEKGGGEQLKGQY
                     ADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIARRT"
     gene            complement(19709..20197)
                     /locus_tag="QBA20_002408"
     CDS             complement(19709..20197)
                     /locus_tag="QBA20_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDQ6292910.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            complement(20221..20400)
                     /locus_tag="QBA20_002409"
     CDS             complement(20221..20400)
                     /locus_tag="QBA20_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292911.1"
                     /translation="MSRVINLGKEKRFPITQELYERLESVIHDYDGEISLCEAIGTLE
                     LLKQSLIEGAKESLT"
     gene            complement(20402..20644)
                     /locus_tag="QBA20_002410"
     CDS             complement(20402..20644)
                     /locus_tag="QBA20_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HDQ6292912.1"
                     /translation="MAEIIPMTEEQKFKLEIYRLLSKNNSAAEEAFAFIGADQLKLEL
                     FKLHYNDGGANPDFTSRTIEAVRKSKEALDLFTTGA"
     gene            complement(20940..21128)
                     /locus_tag="QBA20_002411"
     CDS             complement(20940..21128)
                     /locus_tag="QBA20_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292913.1"
                     /translation="MSSPIMKYFAYQHLPAHLQEVSKPIGELATLMDESLPDSAEKSA
                     GLRKLLEAKDALVRAKLG"
     gene            complement(21179..21646)
                     /locus_tag="QBA20_002412"
     CDS             complement(21179..21646)
                     /locus_tag="QBA20_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDQ6292914.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKAQRDKNAREL
                     KLANAAITDMQMRQRDVAALDAKYTKELANAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLSIRSGIISDQTSLRTLQEYINTQCLK"
     gene            complement(21643..22119)
                     /locus_tag="QBA20_002413"
     CDS             complement(21643..22119)
                     /locus_tag="QBA20_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HDQ6292915.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADNLIAKFKEAGGTVREIEV"
     gene            complement(22103..22426)
                     /locus_tag="QBA20_002414"
     CDS             complement(22103..22426)
                     /locus_tag="QBA20_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDQ6292916.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            complement(23095..23718)
                     /locus_tag="QBA20_002415"
     CDS             complement(23095..23718)
                     /locus_tag="QBA20_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDQ6292917.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(23715..24380)
                     /locus_tag="QBA20_002416"
     CDS             complement(23715..24380)
                     /locus_tag="QBA20_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDQ6292918.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFHWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            complement(24358..24564)
                     /locus_tag="QBA20_002417"
     CDS             complement(24358..24564)
                     /locus_tag="QBA20_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HDQ6292919.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            complement(24561..25172)
                     /locus_tag="QBA20_002418"
     CDS             complement(24561..25172)
                     /locus_tag="QBA20_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HDQ6292920.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     RKKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIG
                     KEAVDEIESNHNRHRWTVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            complement(25165..25341)
                     /locus_tag="QBA20_002419"
     CDS             complement(25165..25341)
                     /locus_tag="QBA20_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HDQ6292921.1"
                     /translation="MLSPSQSLQYQKESIERALTCANCGQKLHVLEVHMCEHCCAELM
                     SDPNSSMYEEEYDG"
     gene            complement(25334..25750)
                     /locus_tag="QBA20_002420"
     CDS             complement(25334..25750)
                     /locus_tag="QBA20_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2591 family protein"
                     /protein_id="HDQ6292922.1"
                     /translation="MTMDYSQLSDFEINRRVCEALDMEEHFFIPDDEADFDSEIPTDE
                     RGPIWQTQKRDINGFRSSNGNCFNPCNNPEYAWPIITESKISIMFDSTDTRYEGEYHE
                     WCDAISSCQKFGIQHQSNPLRAAMIVFLMMQDANNA"
     gene            complement(25747..25923)
                     /locus_tag="QBA20_002421"
     CDS             complement(25747..25923)
                     /locus_tag="QBA20_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HDQ6292923.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKQIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(25920..26447)
                     /locus_tag="QBA20_002422"
     CDS             complement(25920..26447)
                     /locus_tag="QBA20_002422"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HDQ6292924.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDSLDIEFGFWLDSAVSDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAE"
     gene            complement(26444..26884)
                     /locus_tag="QBA20_002423"
     CDS             complement(26444..26884)
                     /locus_tag="QBA20_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HDQ6292925.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            complement(26961..28397)
                     /locus_tag="QBA20_002424"
     CDS             complement(26961..28397)
                     /locus_tag="QBA20_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6292926.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKIIFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQSIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDMLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSFPPGETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            complement(28387..29200)
                     /locus_tag="QBA20_002425"
                     /pseudo
     CDS             complement(28387..29200)
                     /locus_tag="QBA20_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
     gene            complement(29187..29348)
                     /locus_tag="QBA20_002426"
     CDS             complement(29187..29348)
                     /locus_tag="QBA20_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292927.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            complement(29383..29661)
                     /locus_tag="QBA20_002427"
     CDS             complement(29383..29661)
                     /locus_tag="QBA20_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HDQ6292928.1"
                     /translation="MQLTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMAMLLAVLEWGVEDEELAELAKKVAMVLTKEKPQDCGNSFEA"
     gene            complement(29770..29964)
                     /locus_tag="QBA20_002428"
     CDS             complement(29770..29964)
                     /locus_tag="QBA20_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDQ6292929.1"
                     /translation="MIRMTLADYAKIHGQAKAASDFGVIQCAISKAILAGRNIMVTVK
                     PDGSVIGEEVRPFPSNKKNK"
     gene            30071..30787
                     /locus_tag="QBA20_002429"
     CDS             30071..30787
                     /locus_tag="QBA20_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6292930.1"
                     /translation="MENKKSLTTEQLEDAKRLKALYESKKKELGITQYSIADELGITQ
                     GAVGHYLNGRNALNVEVASGFARLLQVSIADFSQSIAAKVAEQAESLKSDANVRYAGE
                     YRAGKRYPVLSSIQAGSWCEACEPYAIKDIDVWLESDAHIQGNAFWLKVEGDSMTAPV
                     GLSIPEGTFVLFDTGREAINGSLVIAKLSDSNEATFKKLIIDGGNKYLKGLNPAWPLV
                     PINGNCKIIGVAIETKLRLV"
     gene            30806..31174
                     /locus_tag="QBA20_002430"
     CDS             30806..31174
                     /locus_tag="QBA20_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6292931.1"
                     /translation="MVGTAIASFLGMLAISTIYGLAHAFIAKSLSEKISQAWAHRSAR
                     FMILVIIAIQGISAFILYGSSLYLLYQGATFTPYTSDYGTLYDGSEDISMAWIVFGLS
                     MAVSVVADIIKVILVLTFAD"
BASE COUNT         7595 a         7719 c         7186 g         8954 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa caaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tctcaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaatctg
      421 tagcaccaat cataactatc tgttttagca agcattaacc gcattcacta agatatagtt
      481 gatggcacaa acagaaagtt gatgaatgat gttgccatat atatgtcaca aatacggcac
      541 aacgatcttc aaacatatag ccacacattc acagaagagc acaaagcctt gcaatccagt
      601 gcaaagattt gtgtgccaga gtttttctca tcaactaccg caagtatcga tcgattgaga
      661 cttggatgat agacttcatg cctttgatta ttagctgata gaagaaatgt taaagctatt
      721 tgcaaagtac acatctatag gcgtgctgaa cacacttata cactgggtgg tttttggtgt
      781 atgtatctat gccgcgcata ccaatcaggc tatggcaaac ttcgcaggtt tcgttgtggc
      841 tgtgagtttt agcttctttg cgaatgcaaa attcacattc aaagcatcaa ctacaacgat
      901 gcgctacatg ttatatgtcg ggttcatggg aacactgagt gctactgttg gatgggttgc
      961 tgatagatgt tcacttcctc caattgtcac tcttgttacc ttctccgcca tcagcttggt
     1021 gtgcggtttc gtctattcaa agttcattgt ctttagggat gcgaaatgaa gatatctctt
     1081 gtcgttcctg tcttcaatga agaagaagcg ataccaattt tttataaaac ggtacgtgaa
     1141 ttcgaagagt tgaagccata tgaagtagaa attgttttca taaatgacgg aagcaaagac
     1201 gctacggagt caatcattaa cgctctggct gtttctgatc cgctagttgt tccgctgtca
     1261 tttacacgca actttggtaa agaaccagca ttgtttgcag ggttagacca tacaaccggc
     1321 gatgccgtga ttccaattga tgttgacctg caagacccga ttgaggttat tcctcatctt
     1381 attgaaaagt ggcaggcagg tgctgacatg gtgcttgcta aacgttcaga ccgctcaact
     1441 gatggacggc tgaaacgtaa gacagctgag tggttctata aattacacaa caaaataagc
     1501 accccaaaaa tagaggaaaa tgtcggagat tttcgactca tgtctcgtga ggttgtggag
     1561 aacattaaac tattgcctga gcgcaatctt ttcatgaaag gcatactgag ctgggttggt
     1621 ggtcagacag atgtcgttga atatgtacgc acagagcgtg ttgctggcat ctcaaaattt
     1681 aatggctgga aattatggaa tctggcactg gaaggtatca caagtttttc aacctttcct
     1741 cttcgcgtat ggacttatat aggcttgttt gttgcaagca tttcattttt atatggtgca
     1801 tggatgatta tagacaccat tgtctttggt aacccagtac gcgggtatcc ctccatgctt
     1861 gtatcaatac ttttcctggg tggagtgcaa ctgatcggga ttggtgttct cggtgaatat
     1921 ataggtagaa tttatttaga aacaaaatca agaccacggt acttaattaa aagcagaaaa
     1981 taacaggatg atcttatgaa gatattcata atgcgcaatt ggcacaagat atcaattttt
     2041 atacttgcat ttactttgat ttggcttaga cgcattgaca ttttgacaaa tgcccagttt
     2101 tgggctgagg atgcagtttt ttggtataaa gatgcatacg aacaaggttt tttcagttca
     2161 ctcataaccc caagaaatgg ttattttcaa acagtatcaa cactcattgt tggtgcgacg
     2221 acatttatta atccaattta cgcaccatta ttatcaaact tttttggaat aataattaga
     2281 gctataatta tatggttctt atttaccgat agatttaaat tcctaagcac cacatcaaaa
     2341 atcttcatct ctgtatattt aatttgcatg cccgggcttg atgaagtcca agccaatata
     2401 acaaatgctc actggtattt atcattgtat gttgcaatga taataatatc tgaagaatcc
     2461 aagtcaaaat tatggaaagc acatgattta ttttttatag tattatctgg attgagcggt
     2521 ccatttatta tattcataat tgcatcatct gtttttaaat atttacactt atcaaaagga
     2581 gtaataagca taagaggttt atacttattt tatactcgac tgccatattt ggcaatgatc
     2641 ttgtgtgggc tgatacaggc aacatcaata attcttacat ttaatggaac aagatcacat
     2701 gctccattag gattcagttt tgatgttatg tcatctataa tatcatctaa tgtatttctt
     2761 ttttcatttg tcccttggaa tatcgctaat gctggatggg acaatcacct tttatcgtac
     2821 acactatcaa ctttagtaat tacattatgt gttttcattt atattaaagg aaattggcaa
     2881 atgaaagtgt ttgccaccct tccaatactt attgtcgcat tttctatggc aaaacctcaa
     2941 ctggcagatt ctgttcctca actgccaaca cttgcaaccg gaaacgggtc aaggtatttt
     3001 gtaaatattc atattgcaat tttttcatta atatgcgttt gtttgtttca atgcataaag
     3061 aataaaacct taaaaatatt ctttaaaatc tatgtttcag tcatccttat tgcaatgata
     3121 aaattaaact tttttataac ccctcttcct gacatgaact ggtctcaagg agcagaatta
     3181 ataaataaag caaaacatgg agaagccgtc tcatttacag tgctccctcc atggttaact
     3241 cttgatttaa taaaaaaata aaacttcaca ttaataaata acaatgaatc cgcctcccta
     3301 cgtattcccg tagggaggct gtcgtgttta tagtagcctg gtaatgctaa atgcaaacga
     3361 actagagatt cctgctccgc taagcgtatt gcgcagggtc accctaaatg tatctacacc
     3421 aatataagta acatcacaac taactgaatc aggcgcagat ataggtgtta tctgaaccac
     3481 ataaccatta gtagctgctg cgagcggatg agaaaacgtt acatcataca caccagtagc
     3541 atgcgcgtta acggatgaaa cacccggcgc aatgtctaca ctaccattgg cgttaattct
     3601 ccctgagcgc gtaactgact ctgcagaaac cgatgtggcc ttactggtta tttccataaa
     3661 ggtgcaacca atatcgatca attcgcaatt gttccctgtt atccagtatg gtcgatctgc
     3721 acttggctga taatcccctg cgctctggaa cgttgtgcca ttgagtaaga ctataacttt
     3781 tcccccacct gagcttgtta cttgtatatt cgtgtgcgtg tagcgttcac tgctcacgcg
     3841 atgaaaattt ccaccgttta taactaccgt tacaggtcgc gtcccagtat tatctatagc
     3901 aaggtctgcc cccccggcat tagcctcgaa gtatgggttg tttaacacca aagcacacga
     3961 accgttaagg ccatttatgg ctaattgcgc accacctgta ccggaatccc cctgagtacc
     4021 atttccttca caagtcaggc tgttgatagt agttcctgcc ccccatctat caccaagtat
     4081 accccattgt gtattctggc ttgcagttaa tcggttaact gtaatggcat taagctctga
     4141 cccttcactg gagttaagtg cgcgccagcc aatacgattt gcctgcaggt acacattatc
     4201 caattcagaa gtcaatacag cgtctgttcg taaaccttcg ccgttggtat gtccgtaaga
     4261 tactatattc tctaacttgg catatgcttt cttggttacg agaagaccgt aagacgttgc
     4321 tgagccttta agcgtaatat ttccgagata atcatacgca tgtactcgct gtgtgccacc
     4381 aactgtatca ccgagcatct ttaagttgaa atccgaggca tggcagttaa atatcgtttc
     4441 tgccatattt tctccggaat aatgagcacg gactcctgtc gaaccaataa atttagcgtc
     4501 atagttgccg taatcgagaa cgagtggtga cgcgatatcg tacacccctt gctttatttt
     4561 taatgttcca ccgaaaggcc acgatgtaag cgggtcttga tatatttcat tttttattgc
     4621 aaatagctca tcttgaagat ttttcccgct attagtgcca atgtaagtag ccccttcatc
     4681 agattgaagt aactgcagaa gctgttcagg gtcatacttc agcaaattcg gaaaatagaa
     4741 ctgttgcgaa ccatacgcat catatacagc cattgaatgg ccttgctcag ttacaaattt
     4801 ggcaatctgc ccgttgtaca caggatatcc ggcagcattg ataacaattg gctgagaaac
     4861 agggacgtga gatccgtctt cattctctac ataaacctga atctggtttt caggatttac
     4921 cgggtcagtg tcaatttttc cgatataaat tttgccattg gcatttgctt taaacgagcg
     4981 agctaaagta aataactgac ttggttgtga aacaacaacg ttgtataatt gttctggcat
     5041 aataaacatt ccggagaaaa taatgagtaa gagacgtcac gaggtagtaa ctcgggagcg
     5101 ccttatggag gttctcgatt acaataaaga aacaggaatt ttcacttgga aaaagaaatt
     5161 aagcgccaga ggtgctgttg ggaaagttgc tggcacaatt tcttatggat ataacgcaat
     5221 taatattgat ggcgtaagat attttgcaca tagacttgcc tggctgtatg tatatggcga
     5281 atggcccaaa caagagattg accacattga tagaaacaga agaaataacg caatctcaaa
     5341 ccttagggat gtcagcagag tcgtaaatgc attaaatgtc ggacctcgaa atagcaatgc
     5401 tggaatcaag ggggtaactt tctgtcaagc cagaaatcaa tggcaagctc agataaatgt
     5461 ctcaggtaaa aacataaccc ttgggagatt caacaccatt gatgaagcgg ctatagctta
     5521 taaggcagca aacatggttg ctgaccatct gttgagtagg tgatatctga catttcattg
     5581 ctccagacga atgatatgat gcaaccatga tgtgattgca taccgaaatg gtactattga
     5641 gtatttatcc agtaagttac gatgccattc cacccaattg gtgaggcatc aaggatgtac
     5701 agcaaatacg acgaggcgca gtttcacttg agacttccgc atgaactcca cgcgaaaatt
     5761 aagcagcgtg cgaagatgaa taacaggtcg ctgaactcag agataattgc agcgattgaa
     5821 gaatcattgg ctaaacaaag ctctgcatct gtttacattg acgatgcaga gcgtattgca
     5881 gaccaacaat ccgatatggt taagaaaatt gtctttgata ctctcaaaga gctatataaa
     5941 aaagactgca actaaccatc cgtggctttc cgttacttct ggttatcatc actgttcatc
     6001 cagtacatga tgcctagcct tgatatggtt ttcttatctg attctggaag tgagttgtaa
     6061 aacgatcgcc aagcagaaga gcgtcttaca gttgtatcaa cgatgctttc tggtgcttgt
     6121 cttactgcca gtcttgctgc tttctggaat tctggtgagc taattagctt ttctgcagat
     6181 tccattgatg cagcgccgcc catcgccctg attttgctgg cgacttttgc cccaatttct
     6241 gacccagcaa cagcacctac tgcactgaac ggtcctgaca cagttgaacc aaccgcagtg
     6301 ccaatgcgtt gggcatggtt agcaacaaat tcatttgccg cagtgacgcg attaaaccgt
     6361 ttgacgaact cgtttagtct tcctgtagtt atctcgtaag attttgcatc tttgatagcc
     6421 tttgcgacct tatatacatc gttcagacct gacatagtct cttttggtaa atgtctggca
     6481 agattgcgca tctgcccgtt tgctaacatg ttttgatacc agtcagcaaa ccctgcagga
     6541 ttaaaatcag cgccacgttt tccgttcgaa agcatatccc gaagacctgt tcccagtagc
     6601 tcggttctta gcttcctgga cggcgtgttt tgcatcaatt cacgaaatcc tttggcatcg
     6661 cctttcgaca ttgcctgtag tgctgtagtt gctttgcgag aaacatcacc gttcagcgtt
     6721 ctgccagtta gattaatcat ttgctcttcc atgctttttc gcatctgaac aagcctctga
     6781 gcaacttcaa aatcacgtaa tgcatttgtc tctgacagcg ccgccttttg atcatcggcg
     6841 agcgaaccgt aaagcctcga taaagcagcg cgatcagcat ctttatatgg tccagaattc
     6901 ttatgaagtg catcaccaac aagtcgtcgt tgcttattga gatttgcata agttaatgcg
     6961 ccgttcttgc ctggattaac tgcccgaaag actctctttt caatagggtc aaggtttttc
     7021 atcccgccaa gatcttctgc cacctgtttg agcattgacc ttgttgatgg cgcatcaatt
     7081 tttgcacctg caggcatcgc cttttcaaca ttgcgataaa gctggtcgct tcgtttcatc
     7141 agcgcagaca ttcttccatt gatcgtatca ataaacttgt cgctcatacc aagagcatct
     7201 tttgcaccgg aaacgcgatc aattatctcc cccgcgccct gtgctagttg cctgattgct
     7261 tcattttcct gcacccgtag agcagaaccc gcacgggact tgattgcttg ctcaactgcc
     7321 ttgtattgct cgttcccgga aaagtgtgaa gggagtaatg aatcaacatt aagcctttca
     7381 gcagactcca gaacttcagc ctgaggattg atatctagtt catcaagcga tgaagcgaga
     7441 tttggccttt tttgtgctgc tactgtgcgg acgacctcct ctgggtccct cgtcgcaggc
     7501 gtcattggta caccttgtgc tgctcttgcc tctgcggcat gaaccgcagg atttgttgct
     7561 gcagcggcat caccggagaa tggtgatgtt tctgttggcg cagtaccgga gaatggtgat
     7621 gtttctgttg ccgcagtggt cgctctctgt gcagatgttt tattgccact aattatattt
     7681 gatgcaccac gaacaccatg ggtaacgcca tttatcaggc catgcgtagc aatgttaagt
     7741 ccggcattaa tagcggcatt ttcggcaaaa tcaccccgtt gattcgccgc gtcagcgaga
     7801 gaaccaatga ccatgtttcc agttacacct gctccaggaa caagataacc gcctattgac
     7861 tctccagctt gcgcgtaagg atctgtcggt cggtcgactg gacgatagac atcatccaat
     7921 acttttggcc caccaagccc ctgactgatt gcattaatca ggcttgcgcc gccctgtaat
     7981 acgtcgaatg gtatgtttac cagaccacga ccagcctgct ctgcaatttg ccctgcactt
     8041 tgaccaccag tgagccaatc gccagcttgt tgcatcaatg agggttcttc ccgtgttggt
     8101 gcattattgg cctgattaac tgtttgttgc tgaacagcct gaccagcaaa atactcatca
     8161 atggcggtgc caatatcttc ggtgcttgta ccatcaggaa aggtaaatgt cttaccgttt
     8221 gcagttactt tcatcattcc accgtaaatt gaatgcctga ttttgaggta tatgatccaa
     8281 cctgattccg tggttctcct gaaggtgtcg aatcttgtgc tggcgctgcg tcagtattca
     8341 ttgacatata ccgcttaacg gcactcccca atgattcacc ttttttaaca tccaacccca
     8401 atatctgacc gccattacgc gattgtccag ggttgccatt cgcgctcatc cactcggctt
     8461 taaactcatt aaactgcgcg tttcgtcgct caaggtttgc cattgcatca agccatcttg
     8521 cgaccgtctc agggttatcc atgtcagttg gcgcaccctg ccgaacgatc tcaacgtctt
     8581 tatccgttgc tgggccggga ggtaggaatt taaggacctg actgttaaca agggcatttt
     8641 ggcgaatgcg caaatcacgc aatgttgtat cgcttccggt aagttttgcg aacatgttct
     8701 gtgcgttacc gaacaaacct gtcgttggtt tttctgctct gaactgttga gcaagcgcac
     8761 tcatagaatt ggctgagttt gatgatgctg tggcattgtt tacagccgtc tcgatgcctt
     8821 tttccatgtt tactgacagc ttaggtgctt cgctaatcaa ctgctgagcc ttttcctgcg
     8881 cttgctgcat cttaaacacg aactcttgct gatccagagc caagcgttgt gctgcgatat
     8941 tgtgcccagt cattgctgac tgataggaaa ggttttgccc tctcgcctga agtgcctcgc
     9001 cagcctgatt gctgcggatt gtctctgcca gcctgcctcg gtcaatttca cgaccagcca
     9061 tcttgtcctg aacattgaag taatcaatcg gaccaagcgc ggccattcca aggtgatcaa
     9121 caaactcacc aaatcctgaa gggttctgct gatacatctg agcaacgtta ttagggtcaa
     9181 cgccgacgcg caccagttcc ttggcgttgt tttgcagcca tgattgcatc gcttctggag
     9241 acgatgccgc aaggcgagcg ccagccgcta aggtaccgat ggaattgcgc tggtcttcat
     9301 caatgaatcc catgccttta cgaacggatt caatctggtc tggatattca gtagccaact
     9361 gacgcaaagc accgcgatca ccagacgcat aagcattagc gtacgcctgc tgaaattctt
     9421 tctgccgctg agcctgcttt tcctgctgaa aaacacccgc aatacctgaa agaccttgca
     9481 aagccgtcag cccaacattg ttagcgcctg aacgctcaat atcattgttc tgcctgataa
     9541 gctgaagcgt attgccgatg tcatttacgc tcggagcgtt tgagttgacg ccgccgatac
     9601 cagccaacaa tccgccgttt gttccttgcc aagtagccat gattacccct taaaacaacg
     9661 agccaagcag gccaagtccg ccgccaattg ccgcacctaa tccagtgcca agtcctggaa
     9721 caatagagcc aagagcagcg ccagtcatag cccctgaagc tccgccgcta attgctgtct
     9781 gaagacctga tggtttattg gcattagcag cggcaagagc cgcactttgc tgcgaaatct
     9841 gactcatgtt gttggcatat gtctgcccgg cgtttgcctg accttgcagt gcgccaagac
     9901 caatatttgc cagattctgg tagttgttca tctgaccaga tagccattgc tgaccaagcg
     9961 ttggtgcgat tgttgctaac tgattaccgg ttgcagtgga acccaatcca cctgttgctt
    10021 ccgctgccgc cagactctga tagcgagcct gaccagcaag atctttgtac tgctgagagt
    10081 tgtaatactg gttaagtgcc tgaccttgcc cctgaagaga ggaaagattc tgcaattgtg
    10141 atacgtactg ctgagcgagt ggcgtgaacg gagcaaggtt tttcatgatc gtctgccact
    10201 gctgattctg caggtctgcg gcatacttct gagcttctgc ggcatacttt gcacttttat
    10261 cagaactgcc acctttcccg cctttttcag ggcaataagg ttcctcgccg cgcagttttc
    10321 tgcccagctt aaatgcatat aacatggcta tctcccgtga ttcaggaagt cgattagttc
    10381 ttcgcgtgtg gcgctgtaaa acgtcacgtc atccacgcct ttgaagtatt tcttgatggt
    10441 ccctacacgc ttaaggccaa tcattgcgca gtacatctgc ccgtggcgga atttgcgtgc
    10501 agcgaacgat gtgacgcact gaacggtggt gttagtcaga atgtatcgcc agaacgccag
    10561 cccgatttcc ttgctgaatc cacgaacctc tggcaggtac atggcgtggc aatcgaatgt
    10621 cagcggctga atctcctgat agtaaacaat gccgccaaac tgaccgtgca cgttaacctc
    10681 aaagtaacgg cattcaggct tgtagtcgta tccatcaccg ttgttgctcc cggcaataat
    10741 gtcagggtga tttccgactg cttcgatcag gtcgatgttt cgcgttggtt tgaactgaat
    10801 cattactgct ccgcgattat cttgatggtt gtggcagtaa acgccgcacc atttgactga
    10861 atggttaacg tgcttccatt tgtggcaaga aagccgtctt tatccacgct gaagaacgta
    10921 gctaacagga tgttgtcggt tgttgtcgcc gcattacgac tgctgaccag cgtatcagga
    10981 acagagccgg aaaaggttag ctgcattgac ctgttggcgg ttccgctggg ccacgtcccg
    11041 acgatcgaca gcttgaagaa caaggttttg ttctcgttga acacaaccat cttgttgtta
    11101 acagtgtcga agaatggtgc caacgtcccg gatgacggcg tgagcgtttt cagcaggcta
    11161 acaaggttag tcggcgctgt cgggatggtg actgatacgc cagagtaaac aacctctgat
    11221 ttcttacgag tagtggcata ctcaagagca tcgatgcgcg tttcatggtc tgagagcgtg
    11281 ttttgaatgg cgacaacttc atccgtcaga taatcaatat cgttttctgc tgtcgttaat
    11341 cgtgagtcaa ggctgactat cgctgcttct gcgttagtga tccttgtttc gtggtcctgt
    11401 atcttcgctt cagctgatgc cagtcgaatt tcgtgatcga ccagaatcac atcctgctca
    11461 tcgttcctga cttgtgcgtc ataagcgccc tgcccggcct cgttggcctt gttagccacg
    11521 ttaccaacat cagtgccctg tgcgataacg taaagcagat acgactgcga gaagatattg
    11581 cgtggaagga ctgatgtgtc gagccgtgta gcctgaatga ttaccggcac attgagattc
    11641 gaatccgcca ttactcaatc cttatctgag cgccagacag agtgactggt gacttcgtga
    11701 taatgcgcaa tttgaagccg acatttttcc tgatgcgccc gactcgcttc cacaaaacac
    11761 gtttgtcgta aacgaacggt tcattctgct caatcatctg ctcacgaccg taatttatgc
    11821 cgtcagtggt tgcagagaga aaaaggcggt cagcgtactg agcaacgcca gttgacgatt
    11881 caacctcaag gtcgaacact ctggcgttat ccgctttgaa cagcggagta aacagaagat
    11941 gttcctgttg cttgtcgtac tggctgctga tgtcgaattg caatttcccg gtcacggact
    12001 ccagcttatc gccgcacgtt atctgattgc cttcgtaaat gaagtcgata gcgcggtaca
    12061 catcgtcata cagacctgtt ttcagcacac accattgcgg accattggcg cttgaagatg
    12121 cgtcgtacac gagaacatgg cgcggaaggt ggataatcag taactcatgc gcatcaaacc
    12181 gcaacgattc catcacgcca tcagccagtt catcagcagt gtaggagcgt agtattttct
    12241 caatgctcgc gctggcgatt ggtgatacct gaccggagcc gatgatatat acagacggcg
    12301 cacctgttgc cggattgctg ataaacgcat aggaatcagc aaacggcgtt ttgcagtaag
    12361 ttccggcaat gcctttctgc accatcagcg atggctgtgc gacatacaac gcggcaccaa
    12421 cagtggttgc accagtcagg gagaaatatt caatcgttga tgaaccaaag cagacgatga
    12481 agtctcgcca tgttccgatg ccgatgatgc cgtcaggctg cgattctgcg cgatattgtg
    12541 cgctgtagcg gtcagggtgc gattcgtctt caaggtcagt gataaaccat gaatcagtac
    12601 cgtcttttga ccacgcataa cgcccacgta agcgcgtaat atcacgaacc gaacctaact
    12661 catactgcgt aaacccgctg tctgcaggcc agtttgagac ggttttaacc gtgccatcat
    12721 agcggtattc gaccagttgc ccgttaacgc ctaccgcctg agatgttcga ccatgcgcca
    12781 ttgatacgcg accacttccg gcaacatcac cgacttcact ttcgcctttg tagagcttgc
    12841 cgccacacac gcgataaaca gcactctgcg ccatgttgta ctcaactccg cgcgatacgc
    12901 cgtttacatc agagcgtttg gcaatgcccg ggaatgagcg aagatatccg ctgctgttca
    12961 ggatttcttt gggtgtagcc aacatattca ctggcagata gtcgatatag tcggcgtttc
    13021 taaagtcttt gccgacacct ttcataagcg gaagttgctg aatcggcatt attcgctccc
    13081 gttatcgcaa ggttccttcc gatggaagta attccaaccg ttccacttcg ccaactgatt
    13141 accgctacca acaggcatac ggtttggata acctgactta catttagcgg cttttgctct
    13201 gtccattgca gacagtttga cgagtcgctc tttcccgtat ctggcagtcg ttataagttt
    13261 tgctgacgct tccagcgcat aatctggagc aatgcggcag gcaaggttga aaatgacggc
    13321 atttatagcg ttatttgata atccgtgctc atctcccgga tctggagcga catctgcatc
    13381 agcaaaaatg tagccaacgt tgataccagg tgacacatca ccgccaagcc attcagccat
    13441 catcatttca aggtcgttga cgccatcttc catggactgc ggttcgacat cggttaacgt
    13501 ggcatttgat gccacaccga gcttacgtaa tgccgcaaga actaaatcac ccttcgttgt
    13561 caggttcatc tgctgccgcc ttaggttttc gaccaggctt tttacgctgt ttttcttctt
    13621 gctctggctc tggctctgga tctggatctg gctctgcaac atccttcaga agatcatcag
    13681 gatgtgcaaa ccagccagca tccagatatt cctgaagctc ttcggctttc acgatttcaa
    13741 agtcgtatcc aacgcctttc catttcttca tgtcgccatg acgaaagatc atgtgtgtca
    13801 tgcttgtctc cagataaaaa agggagccga agctccctct ggttatcacg cagtctggtt
    13861 aggcagacca acaccaattg cctctggtcg tacagcacat gctgaatacc acacagcaat
    13921 acggcactta ccagacagag tgttgatatc accctgcgtt gcgaagatgc cgttaacacc
    13981 aatgccagga atgctgaagg aagacgtttt cataccagca aacagttcat gggttaccgg
    14041 gatcggctga gacagcaggc ggattgagtc atcagcccag aacacgttag cggtggttgt
    14101 tgccacgttc agaacgttta ccgtagtggt atcagcaaga gaggtgttta cgttagcgta
    14161 agccttctct tcttttgtca gtgacgcgtc atccagtgca atcggcttcg gcgtgatttc
    14221 gatgtgagta ccatcgatca cacgggtgat tgagaaagta gcatcatcag ttagcacgtt
    14281 cttcgccatc tgagacagga atttcacacc agtgaaactg attttgtcgc cgcgcttaaa
    14341 tccggtggtg gaggatacgg tcaccgttgc aacacggttg tcgacgttct ctttgttacc
    14401 atcggtatca agggtgtatg cctgcggctt aaacttctgc gcaccagaaa cagttacacc
    14461 agtagcggtt gacttggtaa ctgccggaag tttcggtgag cgaagaattt catcaaagcc
    14521 agcaatctga cgctgaatag taccgttgcg atacgcgtct tcagtaacgc gcccgaagat
    14581 gtcaccatct accaggttgc ggcctgcttt gcggtaatcg tcagggttca ggaagtaact
    14641 gatgcccata tcgcggttta gctcacggga gaacatcagg cgctctgcat cagacacaaa
    14701 atcccagcca gacaggccag tagatggacc aattgcgcgg gtatcgtgaa caacaagcga
    14761 gcccatttca gttgcctgtt tggcaatcgc tgactcaatg ttattcgcca gttttttggc
    14821 ggatgcctgg atgcggcgac ggtaagaacg ctcatcacgc aggtcatctg cacgaagctc
    14881 gaagaaatcg ttatccggat cacccatgtt gcattttacg gagagctcca gaatcccggt
    14941 agcgtttcca gttaaatccc agccagtctg agttggcgct tcctgctcaa caggcatcca
    15001 cacggtgttg cttgaacgtt gcatggattc tgccggaggg gtgtattttg tcactttgga
    15061 cgccattggc gtcaggttct ggacggtttc gatgatttca tccagagcat acgtgaccag
    15121 ttgaccttca tttaatgcca ttatcgaatt cctttattca gttgcgcctt gagcttgcgg
    15181 tacgtctcta catccccttt gtttgctgcc gcttccatct gcttttcaat cgcagagata
    15241 tttgcagcaa cagcgtgtcc ctgaatgggt tcatcaggta acggggcttc tgaaacaggc
    15301 ttggctcgag gcttgagagt taaacgttct gacagtcgag tgagttcaat cagcgcggat
    15361 tgcccgtcca tcgccagcaa ctggcgtgtt ttctcaggat tagcaccaag gtgatacatg
    15421 agagcggcgg atttctccgg gaagaggcgc atgatgtcgg caccgactgc tggcggcacc
    15481 agttgcatga atgcatcctc tttctcctga tagtcaggga tattgagctt ttccgctgcg
    15541 tcgtagtgct tacgggctgc ctcgacgtat tgcgctgatt gctgggtgaa ctcctgagtt
    15601 ttgcgaccct gctcggcgac agcctggctt cgtgcgtcca tagccttgat ctgccattca
    15661 ctgtttgcct gctggaaggc agccagtgcg cggctctggt catagtcgta cttagccagt
    15721 gcgtcttcgg aaagataatc gttagggtct ggttgttttg gtaactcagg gttcacccgc
    15781 aggtgctccg gcaactctcc acgcttaacc gcttccatct gctgctcaag ctcacgctgg
    15841 cgtttgcgtt cgatgcggcg acgggcaaat tcagcattag ttgccgggtc ttgttttggt
    15901 ttctcatcgt ctttcaggac aatctcgaag ccttcttcct gacctgcatt gtcgttggca
    15961 ttatcgacaa ctaagccatc agcagatgcc gctgcatgat tgccgggcag ggttaattct
    16021 tcagaagcct gaatgtcggt ggtttggtcc atggttaact ctctcttatt gaggtgtctc
    16081 ggctactccg ccggagggga tttgaacttg acgcataaga ttcgcgaaat ccatgcgttg
    16141 tgaatgagtc tggtctgcat ctttaagaag cagctcagcg ttagcacgag catctttgct
    16201 gcgctgttgc tggaattgac ctacgagctt gaggtactca cgcagttctg cctgcttgtc
    16261 gaggtccata ttgttgaaga tttccgcaat cttcgcggcg ttgagttggt tttgggcttc
    16321 aaccttggca gcttcaacct gaatctgcgc ctgttggttc tctgccttga gcaattcagc
    16381 ctgaccttgc agaaggatac cctgcgcctg aatttgctct gctgatggct gctgcggctg
    16441 ttgttgtgcc tgctgtacca tctccatctc ttcaggtgtt tctggtttct tcagccccat
    16501 catcaccagt tgcttgttcg cgtactctcg catcatctca acgcctttac cgtcaagcag
    16561 cgtgaagtat tgcagcatca gcatctggaa ctctggagta ccttgcggaa ccttggtgag
    16621 taaatcctga atctctgcgc ggttctgttc cttcatgctc tggaaggatg gtccaacgtc
    16681 tgtatagcac tcatagcgac cgcgaatgtc gttgagtgtg accacattgc cggactggta
    16741 atcgacaact tgcgcataga gttgaacgtc tttctcgctt ccatcttcaa gtgtcagcgt
    16801 tacatgacga ggaacgtcat aaatatcatt gaccattgag gcataaatct cgccatcacg
    16861 tcgcattgcg gtagccaggt tatcctgaaa cacgtatgtc tcaaggtctg cccgcatgtt
    16921 cagttgattg acggtatcga aagcgacctg agagtttgct gcctgcgcat ccacaccaag
    16981 actagccacc tctttcactg cgttggtggc agcctcaagc atgtaagcgt tggcttgcgg
    17041 cacttcaggg ttttccatgt aggagattgg accaatcggc aggtcgttac cgttttcatc
    17101 ggtcctgttc tgcagatagt acggatagtc atcatttcca ccgtacatgt attcgtagcc
    17161 ttcgatttgc tcagggaaga aggttggttt cttcttcggt gaacgagcaa caatatcggc
    17221 gttgaacgac atgatcatgt tacgaaggcg ttgaccgtct ttcgtcagcc ttaccactcc
    17281 ttcgtagcac tccttgtcac cagcgaatga ccattcgcca tacactggaa cgattggaat
    17341 atgctctccg gctatcttct cgcggtcttt cagtatctgc gtgcaggtga tgatcgactt
    17401 atacacacgc cgacgcttca ccttgcgctc tgctgcctta atgaatccac gattagccag
    17461 gtcgtcgatg acgtctttga tatcctgctg gtaatagctg actggctcac ctgtcagcgg
    17521 gtcgcggtag atgaagactt tctctttctt ctcttcgacc tcgtaatact cagcgacgta
    17581 gacgacatca ttcgataccc acggaaacag ccatgtatcg ttcggattct ggaaagatgg
    17641 caaggtgtcc ggatcaatac cgtaatcctc tgcgaactct ttccagccat tgcgcgacaa
    17701 ggcgttaatc accgtgcagt gcttagcgtc gctcttatcc atctgcttgc tgttggcgtc
    17761 ccatatgacg tgtgagcagg cttcatgaat tggcaggcgt cggattacct gattgttgct
    17821 tgttggatcg ttgtcttcgt actgcgtgac cagacgccat gcaccaacgc cggactctat
    17881 ctgctcacga acgccaacgt taacggcaat ttttgccgta ttatggcgca tatcagtacg
    17941 atacatcccc atcaacacat cggctgcatc aggattagca ccgtctttgg gtcggaagag
    18001 aacgtcgata gggttccggc gcatctctgc gaccagtttc ctgaccaccg ggcgaacaac
    18061 atcgaattgt ccgcgatatt gcagggtagt gtagtttgat agccagtcat cccattgcga
    18121 cactcggcta aaatacaggt catttgtcgc ctcggttctg gcttcatcgc tcgccatcca
    18181 gtctgcgtca aacttacaca gaatggaatt gagtctgttt tcgtcggcca tttaagttct
    18241 ccgtgcgatg ggcctgattg gggctggtat ctttttctct tttggttttt tgatgtcgcg
    18301 catcattttt gcgaagcggc gcatcatgta tgcatagcga acggctgaga gaacgtcgtc
    18361 gttaagcttg acgatcttcc cgttttcatc acggtgatag aggcggaact cctcaaagaa
    18421 tggctcacag gtgttgaata ctttgaagcg accatcaagc atcatgtcgc gcaattcagt
    18481 gattccaggc tcaacagcat taccgccatc aggccatgtc gcatgctcct gcaacatcat
    18541 aaaaccagca tctgcatact gccctttgag ctgctcaccg ccgcccttct catgctggtt
    18601 tccgtcatga ggccatgcgg ttggcacttt atgcgcccat gatttaacag ctccccatgc
    18661 ctgaacagct gttttttctt tcgccttcca cacgcgtgaa acgtagattg tgtctgcgtc
    18721 cttatcccac caaagctgaa cctgcgcctg cgggtgatcc catccgaaat ccatcccgcc
    18781 aattacgtag aagtgatcag gacattcgaa cggctgacac ttaatcgtct cttccggtat
    18841 ctggaagatt cgaccactac ccatcgtagg gataccgcga gcacgcgcct ctctctcatg
    18901 ctcgggatag gatgcgatga tttgctcttt ctgctcgtcg gtatagtgct cagcgtcata
    18961 gatggtcatg ttgaccactt tctgcgactt gctgggattc ttcaggaact tggtaacaac
    19021 gtcagacatc cccatcagcg gggtaaacgt cagaattgag aattgcccgt atttgttggt
    19081 acgggtaaga ccttcaccat aaatgctgta tggtggttct tcctcaaacc acacgccgtg
    19141 gattgtgtca ccctgccagc gtgcacggcc ttgcgagtat ggtttgaagt agcagattga
    19201 aatgccatct tcaacgccat cagccgtgtg atgcttaacc agaagatgat caacaaggtt
    19261 cggaaagaaa ggagacttct tccagctaat gatgtcctct ttcggtatgg aaccatagcc
    19321 tggctcacca ttctcttcga tacgaccgca caggatgcgt tgagtcgttt tggttacagt
    19381 ctcgtttgtc tcgccaccaa tccaaaagac aacaggctcg tagaaacgct tacctttcca
    19441 ctcaccgcca tatttaccat cagcaggata gccttttgtg cccggataac gccctgtaag
    19501 gtgaaacgcg acttcagcag caccagtaaa tgacttacca agctggttac cagccataaa
    19561 acatcgctct ggatagtcat gtccggcgtc gatgaactca cgctgtttgc tgtatggcgt
    19621 aaattcatat agcaggtgtg tgttccggta gttctcttct tcttcgagta gctcgagcaa
    19681 ttcgatttgc tcttcgtcgc tcaggttatc aagaatcgcg tccagttcca cggttgaata
    19741 gctccttgat acgagagcgt cgcttatcgc gatctccctt atcaggtgtc acgtcttcaa
    19801 cttgcgactg ctctttgagg cccaaatcac gggcgatgat gttagcgttg agaaggtcag
    19861 cggctgcgcc agagaatttc tggtcgtaga tgatgtcttc cgctcgtgat gtgacgtcag
    19921 aaaaaccttc cattgaccgg aaggttcccc atgtttgcct ggtgatatca aggaaggtac
    19981 acaatcctga aatagtcatg gctcgcatct tagggacatt agccttaatt atttctccct
    20041 gatatgaaaa taccttaccc tcccatagcg ggttatcatc agcccactcg aagtattcac
    20101 aacaagcagc ccacagcgcc tcaggcgatt cgaatttagg atttcgccca tgactactgc
    20161 gggcctccca aaatcggttg ccctttggtg ctgccatatt catctcactt aatcgttatt
    20221 tcaggttaag gactctttcg cgccttcaat cagtgactgc ttcagcaatt cgagtgtacc
    20281 aatcgcctcg cataaactga tttcaccatc gtaatcatgg atgacgcttt ccagccgctc
    20341 gtatagctct tgagtaattg ggaatctctt ctccttaccc aaattgatta cgcggctcac
    20401 atcatgctcc ggtggtgaac aggtctaacg cttccttcga tttacgcacc gcttcgatag
    20461 tgcgagatgt gaagtctgga tttgcaccgc catcgttgta gtgcaacttg aacaattcca
    20521 gtttcagctg gtcagcgcca atgaatgcaa aagcttcctc tgccgctgaa ttgttcttag
    20581 atagcagtcg gtaaatttct aacttgaatt tctgttcttc agtcatggga ataatctctg
    20641 ccattgttgg ctccgtttat ccgttaaaag ggatatcagt taagatatcc cgtgtagggt
    20701 ataagccatt gtcgagacca ctcattgaat ggcctctgca ataaccgatg tctttccatc
    20761 agtccgccac cacaaagaat cttttttgcc ataaggctgg aggttcatct ttcagtggct
    20821 gccagtgtta tttccccact ttctggcttg ggttgtttcg cggtactgcc gtaactggtt
    20881 acccagaata aattccggtt tcattatcaa gcccacccgt agataggctt tgtaatgact
    20941 taccccagct ttgctcgcac cagcgcatct ttagcttcga gcagcttgcg gagacctgct
    21001 gacttttcag cactgtccgg cagtgattca tccatcagtg tcgcaagttc accgattggc
    21061 ttacttactt cctgcagatg cgcagggagg tgttgataag cgaaatactt catgattgga
    21121 gatgacatta tttaccctcg gttagtaaaa agcctcgcta ttacgaggct atgattgttc
    21181 atttcaggca ctgcgtgttg atgtattcct gaagcgttct cagtgatgtt tggtcgctga
    21241 tgattccaga tctgatactg agaacgtttc gtccagcaac tggagagagt tcgacggtgg
    21301 catcattgcc catgccggag gcgctggagg tttcggctga ggatggcaca gggcattttc
    21361 ctttgacgag caccctgcca ccattatcaa gcttgcgccg aagagcatca ttttcagctt
    21421 tcgcattagc caactccttc gtgtattttg catcgagcgc agcaacatca cgctgacgca
    21481 tctgcatgtc agtaattgcc gcgttcgcca gcttcagttc tctggcattt ttgtcgcgct
    21541 gggctttgta ggtaatggcg ttatcacggt aatgattaac agcccatgac aggcagacga
    21601 tgatgcagat aaccagagcg gagataatcg cggttactct gctcatacct caatctctct
    21661 gaccgttccg cctgcttctt tgaattttgc aatcaggttg tcagccttat gctcgaactg
    21721 accataacca gcgcccggca gtgaagccca gatattgctg caacggtcga tagcctgacg
    21781 gatatcaccg cgatcaatca tcggtaaagc gccacgctct ttaatctgtt gcagtgccac
    21841 agcgtcctgg cttttcggag agaaatcttt caggccaagc tgcttacgat aggcatccca
    21901 ccaacgggaa agaagctggt aacggcctgc agctgttgat ttgagtttgg ggtttagcgt
    21961 gacaagtttg cgagggtgat ctgagtaatc agtgaatagc tctccgccta caatgacgtc
    22021 ataaccatga tttctggttt tctgacgtcc gttatcagtc ccctctgacc acgccagcat
    22081 atcgaggaac gccttacgtt gattattgat ttccaccatc ttctactccg gcttttttag
    22141 cagcgaagcg tttgataagc gaaccaatcg agtcagtacc gatgtagccg atgaacacgc
    22201 tcgttatata agcgagattg ctacttagtc cggcgaagtc gagaaggtca cgaatgaacc
    22261 aggcgataat ggcgcacatc gttgcgtcga ttactgtttt tgtaaacgca ccgccattat
    22321 atctgccgcg aaggtacgcc attgcaaacg caaggattgc cccgatgcct tgttcctttg
    22381 ccgcgagaat ggcggctaac aggtcatgtt tttctggcat cttcatgtct tacccccaat
    22441 aaggggattt gctctattta attaggaata aggtcggtta ctgatagaac aaatccaggc
    22501 tactgtgttt agtaatcaga tttgttcgtg accgatatgc acgggcaaaa cggcatgagg
    22561 ttgttagcgc aacctcctgc cacccgcttt cacgaaggtc atgtgtagaa ggccgcagcg
    22621 taactatcac tgatgaattc aggatagcca gtggctacgg ctcagtttag gttgtgctgt
    22681 tgctgggcgg cgatgacgcc tgtacgcatt tggtgatccg gttctgcttc cggcattcgc
    22741 ttaattcagc acaacgataa gagcactcgg tgcatttaag ccaagcccca taagggagaa
    22801 tgctcttacc tgttgcacag atataaaaaa tcccgaaacc gttatgcagg ctctaactat
    22861 tacctgcgaa ctgtttcggg attgcatttt gcagacctct cagcctgcga tggttggagt
    22921 tccagacgat acgtcgaagt gaccaactag gcggaatcgg tagtaagcgc cgcctctttt
    22981 tatctcacta ccacaacgag cgaattaacc catcgttggg tcaaatttac ccaactttat
    23041 tcaaaaagtc aatatcatgc cgttaatatg ttgccatccg tggcaatcat gctgctaacg
    23101 tgtgaccgca ttcaagatat tgtctgcgat tgactcttcc ttgtggcatt gcaccaccag
    23161 agcgtcatac agcggcttaa cagtgcgtga ccaggtgggt tgagtaaggt ttgggattag
    23221 catcgttaca gcgcgatatg cggcgcttgc tggcatcctt gaatagccga cacctttgca
    23281 tcttccgcac tctttctcaa caactctccc ccactgctct gtttttgcta tatcaaccgc
    23341 gcggcctgta ccgtggcaat ctctgcatct tgcccccggc gtcgcggcac tacggcaata
    23401 atccgcataa gcgaatgttg cgagcacttg cagtaccttt gccttagtat ttccttcaag
    23461 ctttgccaca ccacggtatt tccccgatac cttgtgtgca aattgcatca gatagttgat
    23521 agccttttgt ttgtcgttct ggctgagttc atgcttaccg cagaatgcag ccattccgaa
    23581 tccggcttgt gattgcgcca tccccatagc agccatcaca tcagtaccgg aaagagagtc
    23641 agaagccgta gcccgtggtg agtcgctcat catcgggctt tttggcgaat gaaatttagc
    23701 tacgctttcg agtctcatgc gccttctccc tgtacctgaa tcaatgtgag gtttccgcag
    23761 aacactgcgc cggtatcgat atacatctgg ttggcaaact tgagtggttt cactgctggc
    23821 gtatgaccaa agatgaacgt gtccgcgcct ttgatttctt tcacgatccc gtcttgtgag
    23881 ttgctgattc gttcgcggtt ccagattacc tgctgatgat caactggctt tccaaactcg
    23941 tattcgtcac aaggataatc ggcgtggcag atgacatatt ttttatcttt actcaccagt
    24001 tcgatgatta acggaagttc atctgcttta tgggcaagag ctttagccag aatttctttg
    24061 tcgtaatcga gattaaagaa ccagccaccg ccattaagca gccagtgatt gacgtttccg
    24121 cgctctgata agccatcaat catcatttgc tcatggtttc cacgtacagc tctgaaccag
    24181 tggaatgtga ttaattccag gcattcaacg ttctctgtac cgcgatcgac caaatcgcca
    24241 accgagataa gcaggtcttt tttggtgtcg aatcctatcg tctccagttt tttcatcagg
    24301 ttcgtgtagc atccgtgcag atcgccaact acccaaatat ttcggtattt gctgccatca
    24361 attctttcgt aatagcgcat ctctttcact ccatccgcga tgaaccatga gaacgtcgtt
    24421 gacgatggcg tgcattttcc cgtctttatc atcaacgtat tttctgaccg taccgcgact
    24481 acatttcagt ctgcgtgcta cttctgtctg gtttccgtat gcttcaacga gcatgtctgg
    24541 aatggttttt actgagaacg tcatgcggcc tcacttctgc tatttcgcag gtctttgagt
    24601 ttctgttggt actctgcctt gatcgccttg cactcttcga cagtccagcg atggcggtta
    24661 tggtttgatt cgatttcgtc tactgcttcc ttcccgatgc gattaatcag ttcgacgcga
    24721 tacggaacga gatttccgct tttgtgctgg ttgcacacca cgcattgctt gtgaatattg
    24781 cgttcatcaa atcggagttg aggtgccgca gcagttgtcc ggtaatgtcc ggcatcccac
    24841 tgagcagacg tgagcgttcc gcacgagata catggtaagt cgcggtctct ttctctgatg
    24901 aaggcgttta cggcttgttg tgcttgttta atccagtaac tgcggggctt taaggcgagt
    24961 tttcgaatct taagtttatc tttctgtttc tgctcctctc gtcgtcgttt cttctctgct
    25021 gctttttccg cttttttgcg ttctttgctt cgtcgttcga gtgctatctt ggttccacac
    25081 tctggagagc accaccactg attagcgaat gcagggtgaa accattcccg acattcatcg
    25141 tttttacatc gtcttcgcgc tggtttagcc atcgtattct tcctcgtaca ttgagctatt
    25201 cggatcgctc atcagttctg cgcagcagtg ctcacacatg tgaacttcca gcacatgcag
    25261 cttctgaccg cagttagcgc acgttaaagc tcgctcgatg ctttctttct ggtattgaag
    25321 ggattgggat gggctaagca ttattggcgt cctgcatcat gagaaagaca atcatggcgg
    25381 cgcgtagtgg attagactga tgctgaattc cgaacttttg acaagatgaa atcgcatcac
    25441 accactcgtg atattcgcct tcatatctcg tatcggtact atcaaacatt atgcttattt
    25501 tgctttcagt gataattggc cacgcgtatt caggattatt gcaaggattg aagcaatttc
    25561 cgtttgaaga acggaagcca ttaatatccc ttttttgcgt ctgccaaata ggacctcttt
    25621 cgtcagtggg gatctcagaa tcgaagtctg cttcgtcatc aggtatgaag aaatgctcct
    25681 ccatatctaa agcctcacat actcgtctgt taatttcaaa atcacttaac tgtgaataat
    25741 ccattgtcat ttcctcgcac gatgtcttag ccaccggata tcccacaggt gagccgtgta
    25801 gttgaaggtt tttacgtcag attcttttgg gatttgcttg cgtttatttc tggagcgttt
    25861 cgttggaagg tatttgcagt tttcgcagat tatgtcggtg atacttcgtc gctgtcgcct
    25921 cattccgccc tcctgacgcc ctgcccgatc gccatcaatg ccgctttgga tacggtagta
    25981 aacatccgtc gaggactaat gaacggtcgc caaatcagca gcatggaacc tttgctgttt
    26041 cccttcttct ccagccctgt cgatggttcg ataaaattaa tccgtccatc agtgatgatg
    26101 cgaacttcgt caacactctc cagagccttg ctgaaccatc cgacagacat atcctctggc
    26161 acaagcatca ctaccgtctg tcgctgttgt atgcactgct cagcggcttt ttccacccac
    26221 ggcctgatat tgctgtacgg tgggttattc cagattgcac cgtgacttac ccactcagaa
    26281 ttgagcgcgt cgtcggcctc agttagccag tgagcgcaca gagcattttt gtcgctcaca
    26341 gctgaatcca gccagaatcc aaactcaata tccagtgaat caaaaagcca aagcggcgtt
    26401 tgccagcagt ccttgtcgtg tgccggcgta tttgatttga tagtcatgca gcccgatctc
    26461 cccatcgcgc tttccattcg agagccagtc gcgcttcgtc tgaccactta acgccacgct
    26521 ctgtaccgaa tgcctgtata agctctaata gctccgcaaa ttcgcttaca cgcatcctgc
    26581 tggttgactg gcctattacc acaaagccat tcccggcaag gttaggaaca acgtcctgct
    26641 gctttaatgc tgcggtaaac acacacttcc agctttctgc atccagccag cgaccatgcc
    26701 attcaacctg acgagagacg tcacctaagc aggcccatag tttcctgttt tggtctaagc
    26761 tgcggttgcg ttcctgaatg gttactacga ttggtttggt tgggtctgga agaatttgct
    26821 gtactgcgtg aatagcgttt tgctgatgtg ctggagatcg aatttcaaag gttagttttt
    26881 tcatgacttc cctctccccc aaataaaaag acctgcgatt accagcaggc ctgttattag
    26941 ctcagtgatg tagatggtca tcagaatcct cctttcttct tggactgcgg ttcctcgcgt
    27001 tcacggcggc gcatttcagc agactgttgg tctgtgtcat aaatagcgcc atttgcctga
    27061 atgcaataca ccgtgccggt attgccatga cgattgagac gaaggattag ttcggtttca
    27121 ccaggtggaa aactgtcatc aaaagcacct tcacgatgga tccccaccca ataatcgcaa
    27181 tcctgttcaa tctgccctgt atctcgtgag tcacttggta gtgggcgttt attggttcgg
    27241 ctttccagtg cgcggttaag ctgcgtcaga agcacaacaa cgcaatcaag ctctttggca
    27301 aggttcttca gtcctttggt gatcatgccg taagcaaggt cgttgcgatc ggccttctca
    27361 gcggtcatta gtgtcaggta atcgaccaga atcatgccaa cacatccttt ttctcgcttg
    27421 attcgacggc tttcgctgac gatttgagcc agagataatc ccggcgtgtc gtcgatgtaa
    27481 agcatgtcga tttcactcaa gcgattggct gtttcgatcg ccctgttgaa gtcaccatcg
    27541 taatcaccct gatagccgtc atcagcgtca tttgtcgccg gaaggtaaaa aatattcggg
    27601 ttaacacctg acttctgtcc taccagtttt tccagtatct ggtcaccggg catttcaagg
    27661 ctgaacatca gagcgggctt tttctcatgc actgcgcagt tgattgccat ctggctgtat
    27721 agcgtcgttt tccccatctt agggcgagcg ccaatgacaa acagagagcc tttcaccaga
    27781 cctttcggtg acagcatcct gtccagcgat gggatccctg tgctcattcc ccgttgttcg
    27841 cctgacgggt caaatcgctt ctcaaggtcg ctaacccagt cttccatgac ctcaccaaat
    27901 gagcgaaggc cgcgacgcga tccggttttt gcatggtctg tcagttgcgt gaaaatcgac
    27961 tgaatagctt cgtacttctg cgttgcagtc attccgttgc gggaatagag caattccgtc
    28021 gcttcagtca tgcggttgat ggcgtagcgt tccattgcgg tttcgcgaac ctgcattgca
    28081 taggcaacga tgtttgctgc gcttggcgtg ttctttgcga tctcagcgat ataagcaaaa
    28141 ccgccaacag acgccgttaa cgatttacgc tccagttcat cgaaaagcgt caggccatct
    28201 actggctttt gctcacggtg cattctggtt atttcttcga aaatgatttt gtgtggccgg
    28261 ctgtaaaatg aatcaggctt cagcatcgcc agaaccttct ggacgcgctc actgctgtca
    28321 tcatccagaa gcaatccacc aatcaccgcc tgctctgcct cgatgctatg gggcggcgca
    28381 taaaaattat cggtcatcgt gttcaccctc acgaactttc aggtaggtat tatcgttaag
    28441 caggaaatca aatccctttt tgtgccagac ggttccgcgt tgatggtttg ggcgttcttc
    28501 gaacatccat cggcaatttt cgcctacgta gctcaaataa tttctccagt cctgcatcgt
    28561 gaacccatgc ccgtcaagct ggcgggttat cactccggct ttgcgccaga acgttcggat
    28621 ctggttttta cgcttgtcat tcagtgcgcg gattcttggc gcttcaggaa ggatttcgtg
    28681 gtaagcatcg acaacatcct gacagctgac ggaaggtttt ttcttgtcag actttttgtc
    28741 tgctgtggca ctctctaata cgtcagtatt agagatatta tttatattat tgtttatgga
    28801 caaccgttgg acaatttttt gcctgaaaat cgtcatattt aacgattgta aacaggctaa
    28861 atttcttccc catcgagcaa atattaagca tccctttcga ctcaaaagtc cgtaataagc
    28921 tccgaacttt gttgtcgggg atgaatgttt ctctgaccag cgacgggcgt ccagttatca
    28981 tctgaccgcg atcaacagtt atcggaccga tatccgtatt gacgacagta gattcgtgat
    29041 tagccttgag gattaagtga agccaaagat gtactgcctg agagtcctta tagagcctgc
    29101 tgtccataaa ctggcggtgt atagagacat accccatact ggatgcctcc tgatgttgta
    29161 cagggttatg cctgtaatca gctaacttaa cgacgcccat gtttcactcc tgctttggct
    29221 agtctgtaaa caccaacaag gcgctctgcg aacgccctgt tatttgctgc ggctaccact
    29281 aatccctcag gtgaatcagg gtgtcgaatc tcttcttttt cctggtattt cttacgacgt
    29341 tttgtcataa ttactcctgt ggattgatcc agtctttcta catcaggcct caaaactgtt
    29401 gccgcagtct tgaggctttt cttttgtcag caccatggct actttctttg ccagctccgc
    29461 taattcctcg tcttcaacac cccactccag cacagccaga agcatggcca tctttgggat
    29521 aaagctgtct ttccatcgcg aaatttgcga ttcattgatc cctaatgcat cagcaacctt
    29581 tcgctgacca cgtacagcaa ttcgatttag gatgttgctt gtaattgcat tcgctttctt
    29641 gcgagtactt gtaagttgca tatgtaagta tttccttaac aaataagaag ttatgcgcac
    29701 caactgatgc gcgttgtatt cccgcatttc ggcgggaatg aggaccatga ctgttaaaga
    29761 gcggtgttac tatttgtttt tcttgttgct tgggaaagga cgaacttcct ctccaatcac
    29821 actgccatca ggctttaccg taaccataat gttacggcct gccagaatgg ccttgctgat
    29881 agcgcactgg attacaccaa agtcactggc tgctttagcc tgtccatgga ttttggcgta
    29941 atcggcaagt gtcattcgaa tcatatgcac actccgttat taaccatgaa caaagaatac
    30001 tacaggtatt caaagcaatc aatactcagg gtatttttag tttaagtacc ttagctatta
    30061 gaattaagct atggaaaata agaaatcact gacgacagaa cagctcgaag acgctaagcg
    30121 gcttaaggct ttgtatgagt caaaaaagaa agagttggga ataacccaat actcaatcgc
    30181 tgatgaactg ggtatcaccc aaggagcggt agggcattat cttaatggca gaaacgcgct
    30241 aaacgttgag gttgcatctg gttttgcacg actgttgcaa gtctcaattg ctgattttag
    30301 ccagtcaatt gctgccaagg ttgcagaaca ggcagaaagc cttaagagcg atgccaacgt
    30361 aaggtatgca ggggaataca gagcaggaaa gaggtatccg gtgttaagca gtatccaggc
    30421 tggctcgtgg tgtgaagcat gcgaaccata cgccattaaa gacatagatg tttggcttga
    30481 gtctgacgcg catattcaag gtaatgcgtt ctggcttaaa gtggaaggtg attcaatgac
    30541 ggcaccggtt gggttaagca ttccagaggg aacattcgtt cttttcgata ccggaaggga
    30601 ggcgatcaac ggcagcttgg tcatagcaaa actttctgac tctaacgaag caacattcaa
    30661 gaagctgata atcgacggcg gaaataaata cctcaaggga cttaatcctg catggcctct
    30721 cgtgccaatc aatggaaact gcaagattat aggcgttgca attgagacaa aactcagact
    30781 ggtttgatca cgcaaggggg cgcttatggt tggaaccgct atagcaagct ttttggggat
    30841 gttggcaatc tcaacaattt acggcttagc gcatgctttt attgcgaaat ctctatcaga
    30901 aaaaataagc caggcttggg cgcatagatc agctcgtttc atgattctag tgatcatagc
    30961 aatacaaggg atatctgcat ttatcctcta tggatcaagc ttatacctat tgtatcaagg
    31021 cgcgacattt acgccttaca ccagtgatta cggaacccta tacgatggta gtgaagacat
    31081 ctctatggct tggatcgtct ttggtttatc tatggccgtg tctgttgtag cagacatcat
    31141 taaggtaatt ctcgtcttaa ccttcgctga ctaacccata atcccggcag caatagctat
    31201 cgggatccac ttcacatatc ccgcataaaa agcactgaac aagcagacac cgaaacaaat
    31261 aaatatcctt tgtattcatt tgtttatcat tatttcatca aaaataaata ccttgggtat
    31321 ttacacaata aaatacctac agtattcttt agccatcagc aggacgctgg aagccaaacg
    31381 gaacagattg gcaggctctt taacattgat gggattgtcc cgccgaaatg cgggaaccaa
    31441 agagtagttg gctt
//
LOCUS       DAOFMX010000048        31009 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.048, whole genome shotgun sequence.
ACCESSION   DAOFMX010000048 DAOFMX010000000
VERSION     DAOFMX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.048"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            389..2047
                     /locus_tag="QBA20_002431"
     CDS             389..2047
                     /locus_tag="QBA20_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDQ6292932.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="QBA20_002432"
     CDS             complement(2044..3000)
                     /locus_tag="QBA20_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HDQ6292933.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="QBA20_002433"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="QBA20_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDQ6292934.1"
                     /translation="MKKRKTVKKRYVIALVIVIAELITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="QBA20_002434"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="QBA20_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDQ6292935.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="QBA20_002435"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="QBA20_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDQ6292936.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="QBA20_002436"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="QBA20_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDQ6292937.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="QBA20_002437"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="QBA20_002437"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDQ6292938.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9799..9886)
                     /locus_tag="QBA20_002438"
     tRNA            complement(9799..9886)
                     /locus_tag="QBA20_002438"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9850..9852),aa:Ser,seq:gga)
     gene            complement(10140..10358)
                     /gene="infA"
                     /locus_tag="QBA20_002439"
     CDS             complement(10140..10358)
                     /gene="infA"
                     /locus_tag="QBA20_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDQ6292939.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10643..11347)
                     /gene="aat"
                     /locus_tag="QBA20_002440"
     CDS             complement(10643..11347)
                     /gene="aat"
                     /locus_tag="QBA20_002440"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDQ6292940.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11389..13110)
                     /gene="cydC"
                     /locus_tag="QBA20_002441"
     CDS             complement(11389..13110)
                     /gene="cydC"
                     /locus_tag="QBA20_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDQ6292941.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13111..14877)
                     /gene="cydD"
                     /locus_tag="QBA20_002442"
     CDS             complement(13111..14877)
                     /gene="cydD"
                     /locus_tag="QBA20_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDQ6292942.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQSVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15000..15965)
                     /gene="trxB"
                     /locus_tag="QBA20_002443"
     CDS             complement(15000..15965)
                     /gene="trxB"
                     /locus_tag="QBA20_002443"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDQ6292943.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16509..17003
                     /gene="lrp"
                     /locus_tag="QBA20_002444"
     CDS             16509..17003
                     /gene="lrp"
                     /locus_tag="QBA20_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDQ6292944.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17138..21049
                     /gene="ftsK"
                     /locus_tag="QBA20_002445"
     CDS             17138..21049
                     /gene="ftsK"
                     /locus_tag="QBA20_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDQ6292945.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLL
                     MRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVV
                     NYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPN
                     KKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKS
                     VGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWC
                     VNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEP
                     YIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANI
                     PTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAV
                     VQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQ
                     RQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21204..21815
                     /gene="lolA"
                     /locus_tag="QBA20_002446"
     CDS             21204..21815
                     /gene="lolA"
                     /locus_tag="QBA20_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDQ6292946.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21826..23169
                     /gene="rarA"
                     /locus_tag="QBA20_002447"
                     /gene_synonym="mgsA"
     CDS             21826..23169
                     /gene="rarA"
                     /locus_tag="QBA20_002447"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDQ6292947.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23260..24552
                     /gene="serS"
                     /locus_tag="QBA20_002448"
     CDS             23260..24552
                     /gene="serS"
                     /locus_tag="QBA20_002448"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDQ6292948.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24792..27236
                     /gene="dmsA"
                     /locus_tag="QBA20_002449"
                     /pseudo
     CDS             24792..27236
                     /gene="dmsA"
                     /locus_tag="QBA20_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27247..27864
                     /gene="dmsB"
                     /locus_tag="QBA20_002450"
     CDS             27247..27864
                     /gene="dmsB"
                     /locus_tag="QBA20_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDQ6292949.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27866..28657
                     /locus_tag="QBA20_002451"
     CDS             27866..28657
                     /locus_tag="QBA20_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDQ6292950.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28693..29319)
                     /locus_tag="QBA20_002452"
     CDS             complement(28693..29319)
                     /locus_tag="QBA20_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDQ6292951.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29634..30782
                     /locus_tag="QBA20_002453"
     CDS             29634..30782
                     /locus_tag="QBA20_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6292952.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7637 a         7588 c         8330 g         7454 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc gaactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcgggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9721 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9781 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9841 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9901 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
     9961 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10021 atcgagacaa gttatggact tatatttcca aagtacttca tacatatcac aaaataaaaa
    10081 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10141 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10201 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10261 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10321 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10381 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10441 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10501 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10561 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10621 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10681 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10741 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10801 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10861 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10921 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    10981 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11041 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11101 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11161 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11221 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11281 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11341 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11401 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11461 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11521 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11581 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11641 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11701 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11761 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11821 cagcgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11881 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    11941 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12001 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12061 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12121 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12181 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12241 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12301 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12361 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12421 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12481 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12541 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12601 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12661 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12721 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12781 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12841 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12901 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    12961 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13021 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13081 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13141 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13201 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13261 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13321 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13381 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13441 aacaggtgta tcaacgcttt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13501 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13561 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13621 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13681 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13741 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13801 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13861 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13921 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    13981 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14041 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14101 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14161 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14221 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14281 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14341 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14401 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14461 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14521 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14581 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14641 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14701 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14761 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14821 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14881 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    14941 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15001 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15061 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15121 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15181 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15241 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15301 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15361 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15421 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15481 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15541 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15601 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15661 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15721 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15781 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15841 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15901 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    15961 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16021 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16081 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16141 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16201 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16261 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16321 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16381 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16441 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16501 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16561 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16621 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16681 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16741 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16801 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16861 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16921 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    16981 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17041 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17101 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17161 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17221 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17281 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17341 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17401 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17461 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17521 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17581 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17641 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17701 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17761 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17821 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17881 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    17941 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18001 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18061 tccattatta aacggtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18121 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18181 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18241 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18301 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18361 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18421 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18481 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18541 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18601 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18661 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18721 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18781 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18841 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18901 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    18961 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19021 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19081 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19141 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19201 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19261 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19321 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcgcc
    19381 acagcagcaa tatcagcagc cgcaacaacc agttgcgccg cagccgcagt atcaacagcc
    19441 gcaacaaccg gttgcaccgc agccgcagta tcagcagcca caacagccag ttgcgccaca
    19501 gccgcaggat accctgcttc atccgctgtt gatgcgtaat ggcgacagcc gtccgttgca
    19561 caaaccgacg acgccgctgc cttctctgga tttgctgaca ccgccgccga gcgaagtgga
    19621 gccggtagat acctttgcgc ttgaacaaat ggcgcgtctg gtggaagcgc gtctggctga
    19681 tttccgtatt aaagccgatg tcgttaatta ctctccgggg ccagttatca ctcgctttga
    19741 acttaacctg gctcctggcg taaaagcggc gcgcatttct aacttgtcac gggaccttgc
    19801 ccgttcactt tcgacggtgg cggtgcgtgt cgttgaagtt attcctggca aaccctatgt
    19861 aggtctggag ttaccaaata aaaaacgaca aaccgtttat ctgcgcgaag ttttggataa
    19921 cgccaaattc cgcgataatc cgtcgccatt aaccgtggtg ctgggtaaag atatcgccgg
    19981 tgagccggtg gttgccgatc tggcgaaaat gccgcacttg ttggttgcgg ggactaccgg
    20041 ttccggtaaa tctgtcggtg tgaacgcgat gatcctgagc atgctttata aagcacagcc
    20101 agaagatgtg cgtttcatca tgatcgaccc gaaaatgctg gagctttcgg tttatgaagg
    20161 cattccgcac ctgttaacgg aagtcgttac tgatatgaaa gatgccgcca acgcgctgcg
    20221 ctggtgtgtt aacgagatgg agcgtcggta taaactgatg tctgcgctgg gtgtgcgtaa
    20281 tctggcgggt tataacgaaa aaattgctga agccgatcgc atgatgcgtc cgattccaga
    20341 cccgtactgg aagccgggtg acagtatgga tgcccagcat ccggtgctga aaaaagaacc
    20401 atacattgtc gtgttggttg acgaatttgc cgacctgatg atgacggtag gtaaaaaagt
    20461 ggaagagctg atagctcgtc tggcgcaaaa agcccgtgcc gcgggtatcc acctcgtact
    20521 ggcaactcag cgtccatcgg ttgatgttat tactggtctg attaaagcga atattccgac
    20581 ccgtatcgcc tttaccgtat ccagtaagat tgactcacgt accattcttg atcaggctgg
    20641 cgcggaatca ctgctgggta tgggggatat gctctactct gggccgaact ccacgttgcc
    20701 ggtacgtgtc catggtgctt ttgttcgcga tcaggaagtt catgccgtgg tgcaggactg
    20761 gaaagcgcgt ggtcgcccac agtatgttga tggcatcacc tccgacagcg aaagcgaagg
    20821 tggtgcgggt ggtttcgatg gcgctgaaga actggatccg ttgttcgatc aggcggtgca
    20881 gtttgtcact gaaaaacgca aagcgtcaat ttctggcgta cagcgtcagt tccgcattgg
    20941 ttataaccgt gcagcgcgta ttatcgaaca gatggaagcg caggggattg tcagcgaaca
    21001 ggggcacaac ggtaatcgtg aagtgctggc cccaccgccg tttgactaat taatgcattg
    21061 ccggataagg cgcggtagcg tcgcatccgg cactctatca actgaaaatt cagtattttc
    21121 ttctttcctc aagctgatta ttagcctgga atagagagta gagggaactc ccgatcggga
    21181 gtgacgtaat ttgaggaata atgatgaaaa aaattgctat cacctgtgca ttactctcaa
    21241 gcttagtagc aagcagcgtt tgggctgatg ccgcaagcga tctgaaaagc cgcctggata
    21301 aagtcagcag cttccacgcc agcttcacac aaaaagtgac tgacggtagc ggcgcggcgg
    21361 tgcaggaagg tcagggcgat ctgtgggtga aacgtccaaa cttattcaac tggcatatga
    21421 cacaacctga tgaaagcatt ctggtttctg acggtaaaac actgtggttc tataacccgt
    21481 tcgttgagca agctacggca acctggctga aagatgccac cggtaatacg ccgtttatgc
    21541 tgattgcccg caaccagtcc agcgactggc agcagtacaa tatcaaacag aatggcgatg
    21601 actttgtcct gacgccgaaa gccagcaatg gcaatctgaa gcagttcacc attaacgtgg
    21661 gacgtgatgg cacaatccat cagtttagcg cggtggagca ggacgatcag cgcagcagtt
    21721 atcagctgaa atcccagcaa aatggggctg tggatgcagc gaaatttacc ttcaccccgc
    21781 cgcaaggcgt cacggtagat gatcaacgta agtagaggca cctgagtgag caatctgtcg
    21841 ctcgattttt cggataatac ttttcaacct ctggccgcgc gtatgcggcc agaaaattta
    21901 gcacagtata tcggtcagca acatttgctg gctgcgggga agccgttgcc gcgcgctatc
    21961 gaagccgggc atttgcattc tatgatcctc tgggggccac cgggtaccgg caaaacaacc
    22021 ctcgctgaag tgattgcccg ctatgcgaac gctgatgtgg aacgtatttc tgccgtcacc
    22081 tctggcgtga aagagattcg cgaggcgatc gagcgcgccc ggcaaaaccg caatgcaggt
    22141 cgccgcacta ttctttttgt tgacgaagtt caccgtttca acaaaagcca gcaggatgca
    22201 tttctgccac atattgaaga cggcaccatc acttttattg gcgcaaccac tgaaaacccg
    22261 tcgtttgagc ttaattcggc actgctttcc cgtgcccgtg tttatctgtt gaaatccctg
    22321 agtacagagg atattgagca agtactaact caggcgatgg aagacaaaac ccgtggctat
    22381 ggtggtcagg atattgttct gccagatgat acacgacgtg ccattgcaga actggtgaat
    22441 ggcgacgcgc gccgggcgtt aaatacgctg gaaatgatgg cggatatggc cgaagtctat
    22501 gatagcggta agcgggtcct gaagcctgaa ttactgaccg aaatcgccgg tgaacgtagc
    22561 gcccgctttg ataacaaagg cgatcgcttt tacgatctaa tttccgcact gcataagtcg
    22621 gtacgtggta gcgcacccga tgcggcgctg tactggtatg cgcgaattat taccgctggt
    22681 ggcgatccgt tatatgtcgc gcgtcgctgt ctggcgattg cgtctgaaga cgtcggtaat
    22741 gccgatccac gggcgatgca ggtggcaatt gcggcctggg attgctttac tcgcgttggc
    22801 ccggcggaag gtgaacgcgc cattgctcag gcgattgttt acctggcctg cgcgccaaaa
    22861 agcaacgctg tctacactgc gtttaaagcc gcgctggccg atgctcgcga acgcccggat
    22921 tatgacgtgc cggttcattt gcgtaatgcg ccgacgaaat taatgaagga aatgggctac
    22981 gggcaggaat atcgttacgc tcatgatgaa gcaaacgctt atgctgccgg tgaggtttac
    23041 ttcccgccgg aaatagcaca aacacgctat tatttcccga caaacagggg ccttgaaggc
    23101 aagattggtg aaaagctcgc ctggctggct gaacaggatc aaaatagccc cataaaacgc
    23161 taccgttaat gttatcgttg cggtaatgtt gttactgtat ccctgtggtc gcaggctgtg
    23221 gccacatctc ccatttaatt cgataagcac aggataagca tgctcgatcc caatctgctg
    23281 cgtaatgagc cagacgcagt cgctgaaaaa ctggcacgcc ggggctttaa gctggatgta
    23341 gataagctgg gcgctcttga agagcgtcgt aaagtattgc aggtcaaaac ggaaaacctg
    23401 caagcggagc gtaactcccg atcgaaatcc attggccagg cgaaagcgcg cggggaagat
    23461 atcgagcctt tacgtctgga agtgaacaaa ctgggcgaag agctggatgc agcaaaagcc
    23521 gagctggatg ctttacaggc tgaaattcgc gatatcgcgc tgaccatccc taacctgcct
    23581 gcagatgaag tgccggtagg taaagacgaa aatgacaacg ttgaagtcag ccgctggggt
    23641 accccgcgtg agtttgactt tgaagttcgt gaccacgtga cgctgggtga aatgcactct
    23701 ggcctcgact ttgcagctgc agttaagctg actggttccc gctttgtggt aatgaaaggg
    23761 cagattgctc gcatgcaccg cgcactgtcg cagtttatgc tggatctgca taccgaacag
    23821 catggctaca gtgagaacta tgttccgtac ctggttaacc aggacacgct gtacggtacg
    23881 ggtcagctgc cgaaatttgc tggcgatctg ttccatactc gtccgctgga agaagaagca
    23941 gacaccagta actatgcgct gatcccaacg gcagaagttc cgctgactaa cctggtgcgc
    24001 ggtgaaatca tcgatgaaga tgatctgcca attaagatga ccgcccacac cccatgcttc
    24061 cgttctgaag ccggttcata tggtcgtgac acccgtggtc tgatccgtat gcaccagttc
    24121 gacaaagttg aaatggtgca gatcgtgcgc ccagaagact caatggcggc gctggaagag
    24181 atgactggtc atgcagaaaa agtcctgcag ttgctgggcc tgccgtaccg taaaatcatc
    24241 ctttgcactg gcgacatggg ctttggcgct tgcaaaactt acgacctgga agtatggatc
    24301 ccggcacaga acacctaccg tgagatctct tcctgctcca acgtttggga tttccaggca
    24361 cgtcgtatgc aggcacgttg ccgcagcaag tcggacaaga aaacccgtct ggttcatacc
    24421 ctgaacggtt ctggtctggc tgttggtcgt acgctggttg cagtaatgga aaactatcag
    24481 caggctgatg gtcgtattga agtaccagaa gttctgcgtc cgtatatgaa cggactggaa
    24541 tatattggct aatacccaat ttttctgaat ctaaaaagcg cctttagggc gctttttttg
    24601 tctccctttg ataccgaaca ataattactc ctcacttaca cgtaatacta ctttcgagtg
    24661 aaaatctacc tatctctttg attttcaaat tattctatgt atacaagcct atatagcgag
    24721 ctgctataga aataattaca caatacggtt tgttactgga atcaatcgtg atcaagcttg
    24781 agtgagccat tatgaaaacg aaaatccctg atgcggtatt ggctgctgag gagagtcgcc
    24841 gtggtttggt aaaaacgaca gcgatcggcg gcctggcaat ggccagcagc gcattaacat
    24901 taccttttag tcggattgcg cacgctgtcg atagcgccat tccaacaaaa tcagacgaaa
    24961 aggttatctg gagcgcctgt acagttaact gtggtagtcg ctgcccgcta cgtatgcacg
    25021 tcgtggacgg tgaaatcaaa tatgtcgaaa cggacaatac cggcgatgac aattacgacg
    25081 gcctacacta ggttcgcgcc tgcctgcgtg ggtgttccat gcgtcaccgt gtctacaatc
    25141 cggaccgcct gaaatatccg atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc
    25201 gcattagctg ggaagaagcc tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag
    25261 agtacggcaa cgagtctatc tatctgaact atggcaccgg tacgctgggc ggcaccatga
    25321 cccgctcctg gccgccggga aataccctgg tcgcgcgcct gatgaactgc tgcggcggtt
    25381 atcttaacca ttacggtgac tactcttccg cgcaaattgc ggaaggtttg aaccatacct
    25441 acggcggctg ggcagatggt aacagcccgt cggatatcga aaacagtaaa ttggtagtgc
    25501 tgtttggtaa taacccaggc gaaacgcgaa tgagtggcgg tggggtgact tactatcttg
    25561 aacaggcacg ccagaaatct aatgctcgca tgatcatcat cgatccgcgc tataccgaca
    25621 ccggtgccgg gcgcgaagat gagtggatcc ctattcgtcc aggaacagat gccgcactgg
    25681 ttaacggtct ggcgtacgtc atgatcacta aaaacctggt ggatcaggca ttcctcgata
    25741 aatattgcgt tggctatgac gagaaaaccc tgcccgccag tgcgccgaaa aatggtcatt
    25801 ataaagctta tattctgggt gaagggccag atggcgtggc taaaacaccg gaatgggcct
    25861 cgcaaatcac cggtgttccg gcagacaaaa tcatcaaact ggctcgtgaa atcggcagta
    25921 ccaaaccggc gtttatcagc cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg
    25981 caacccgtgc tatctcgatg ctggcgattc tgaccggtaa cgttggtatt aatggaggga
    26041 acagcggcgc gcgtgaaggt tcatacagct taccgtttgt ccgtatgccg accttggaaa
    26101 acccgatcca gaccagcatt tcgatgttta tgtggaccga tgccattgaa cgtggcccgg
    26161 aaatgacggc gctgcgtgat ggtgtgcgcg ggaaagataa gctggatgtg ccgatcaaaa
    26221 tgatctggaa ctatgccggt aactgcctga ttaaccagca ttctgaaatc aaccgtaccc
    26281 atgaaatcct ccaggatgat aagaagtgcg agctgattgt ggttatcgac tgccacatga
    26341 cctcatcggc gaaatatgct gacatcctgc tgcctgactg caccgcttcc gaacagatgg
    26401 actttgcact ggatgcatcc tgcgggaata tgtcttacgt gattttcaac gatcaggtga
    26461 ttaaaccgcg ctttgaatgt aagaccatct atgaaatgac cagcgaactg gcaaaacgtc
    26521 ttggcgttga gcaacagttt actgaaggcc gtacccagga agagtggatg cggcatctgt
    26581 atgcccagtc gcgggaagcg attcctgaac tgccaacgtt tgaagagttc cgcaagcagg
    26641 ggatctttaa aaagcgcgac ccacaagggc atcacgttgc ttataaagcc ttccgtgaag
    26701 atccgcaggc aaacccactg actacgccat cgggcaaaat tgagatttat tcgcaggcgc
    26761 tggctgacat tgccgctacc tgggaattgc cagaaggcga tgtgatcgat ccactgccga
    26821 tctacacgcc gggctttgaa agttatcagg atccgctgaa caaacagtat ccgctgcagc
    26881 ttacaggttt ccactataaa tctcgcgttc actcaactta cggcaacgtt gatgtgctga
    26941 aagcggcttg ccgtcaggaa atgtggatca acccgcttga tgcccaaaaa cgcggtatcc
    27001 acaacggcga taaagtcagg atctttaacg atcgtggtga ggttcatatt gaggcgaaag
    27061 tgacgccacg aatgatgccg ggcgtggtcg cactgggtga aggcgcctgg tatgacccgg
    27121 atgcaaaacg tgtcgataag ggcggttgta tcaacgtact gaccactcaa cgtccgtctc
    27181 ctctcgctaa ggggaatccg tcacatacaa accttgttca ggttgaaaag gtgtaaggag
    27241 taaccgatga caacccagta tggatttttt attgattcca gccgttgcac cggttgcaaa
    27301 acctgcgagc tggcttgtaa agactacaaa gatttgacgc cagaagtcag tttccgccgc
    27361 atttatgaat atgctggcgg cgactggcag gaagataacg gtgtctggca ccagaacgtg
    27421 tttgcctact atctgtcgat ttcgtgcaac cactgcgaag atccggcttg tactaaagtc
    27481 tgcccgagcg gtgcgatgca taaacgcgaa gatggttttg ttgtggtcga tgaagatgta
    27541 tgcattggct gccgttactg tcatatggct tgcccgtatg gcgcaccgca atataacgaa
    27601 acgaaaggcc atatgaccaa atgcgatggt tgttatgacc gtgttgcgga gggtaaaaag
    27661 ccgatctgtg ttgaatcttg cccgctgcgg gcgctggatt tcggccctat cgacgagctg
    27721 cgtaaaaaac atggcgatct ggcggccgtt gcgccgttgc cgcgagctca ctttaccaaa
    27781 ccgaatattg tgatcaaacc caatgccaat agccgcccga ccggggatac cactggctat
    27841 ctggcaaacc cgaaggaggt gtgagatggg aagtggatgg catgaatggc cgctgatgat
    27901 cttcacggtc ttcgggcaat gtgtggcggg tggttttatc gttctggctt tggcgctgct
    27961 caaaggcgac ctgcgagcag aagcccagca gcgtgttatc gcctgcatgt ttggcttatg
    28021 ggtactgatg ggcattggct ttatcgcctc tatgctccat cttggttcac cgatgcgcgc
    28081 ctttaactcg ctcaaccggg taggggcttc agcactcagt aacgaaatcg ccagcggttc
    28141 gatctttttt gctgtaggcg gcatcggctg gttgctggca atgctgaaaa agctgtcacc
    28201 ggcattgcgt acgctatggc tggtggtgac gatggttctt ggcgtcgtct ttgtctggat
    28261 gatggtgcgt gtgtataaca gcattgatac cgttccgacc tggtacagca tctggacgcc
    28321 gatgggctgg gcgatgcgtc tgctgccagc catttctgta ctggcactgg tggtgagtgg
    28381 cgtgatgtca gtgatgcagg gcacagagct ggcaactatt catagttctg tgcagcaggc
    28441 cgcagcgctg gtgccggact atggtgcgct gatgtcctgg cggatcgtgc ttttggccgt
    28501 tgccctgtgc ttgtggattg caccacagct aaaaggttat cagcctgcgg ttccgctact
    28561 atccgtctca ttcattctgc ttctggcagg ggaattaatc ggtcgcggcg tattctatgg
    28621 tttgcatatg accgtgggga tggccgtcgc aagctaatca gtaacaaccg gggtttcggc
    28681 cccggttctc ttttatttct gcttcgttaa cgtgtcataa ctggtcatca aattacgata
    28741 atcaggaata tggttagaga acaacgtcgc cagtccttca atatcattac gccagtcacg
    28801 gtgcagctcg caagccacgc cgaaccatgt catcaattgc gcaccagctt gcgacatgcg
    28861 atcccacgcc gaatgccggg taatttcatt aaaggtccct gatgcatcag tcacgacaaa
    28921 gacatcaaac ccttcttcaa tggctgaaag cgccgggaat gccacgcaaa cttcggttac
    28981 cacaccggca ataattaact gttttttacc tgtcgctttg actgctttta caaaatcttc
    29041 gttatcccag gcgttaatat tgcccgggcg agcaatgtaa ggtgcatcgg ggaattgtgc
    29101 tttcagttct agaactaatg ggccgttagg accggtttca aaactggtgg taagaatagt
    29161 tggtaagttg aaatacttag ccaggtcacc cagcgccagc acgttatttt taaacttatc
    29221 gggttcgata tcccgtacaa gggaaagtaa accagcctgg tgatcaacaa gcaaaacggc
    29281 agcatcattt ttatcaagac gaacatacgg tttggtcatc ctcttctcct ttcgagagtc
    29341 agtggtacgt ctgaaatcgg tcagacgcga tgcattgctc tgaaagcata gacgggaaat
    29401 atgagtttgc tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag
    29461 atcaataaaa acaatcagat gagattaatc atctcttcag cattagcgtt atgaatgagt
    29521 aatataaatc gataataccg ccgctgcgat atgcgcgtat tttcacccat tgacaatgtt
    29581 tttggcggtg gcatgatgcg catgaaattt gaacttcctc acggttttaa ttcatgtcca
    29641 cgtatacccg gcctgtcatg cttttgctgt ctggcctgct tttgttgact ctggcgatcg
    29701 cggtgttaaa tacactcgtg ccgctttggc tcgcccagga acacatgtcc acatggcagg
    29761 taggcgttgt cagctcatcc tattttaccg gcaaccttgt cggtacattg ctgacagggt
    29821 atgtcattaa acgcattggc tttaaccgca gctattatct gtcctccttc atttttgctg
    29881 ctggctgtgc cggccttggc ctgatgattg gtttctggag ctggttggcg tggcgttttg
    29941 tcgcgggcgt cggctgtgcc atgatttggg tggttgttga gagcgcgctg atgtgcagtg
    30001 ggacgtcacg taaccgtggg cgtttgcttg ctgcgtatat gatggtttat tacgtgggaa
    30061 cgtttttagg ccagttactg gtcagcaaag tctcaaccga gctgatgagc gtattgccgt
    30121 gggttacagg tttgacattg gcagggatct taccgctgtt gtttacgcgt gtcctgaatc
    30181 agcaggctga aaaccatgat tcgacgtcaa ttacggcaat gctaaaactc cgtcaggcgc
    30241 ggcttggcgt gaatggctgc attatctcag gaatcgttct gggatctcta tatggcctga
    30301 tgcctctgta cctcaatcac aaaggggtga gcaatgccag tattggtttc tggatggcgg
    30361 tactggtcag tgcgggtatc ctcggacaat ggccgattgg acgtctggcg gataagtttg
    30421 gtcgactgct ggttttgcgt gttcaggtct ttgtcgtcat tctcggcagt attgcgatgc
    30481 ttagccaggc ggcgatggcc ccagcgttat tcatcctcgg tgccgctggc tttacgctat
    30541 atccggtggc gatggcctgg gcttgtgaga aagttgaaca tcatcaactg gtggcgatga
    30601 accaagcctt actgttgagc tataccgtgg gaagtctgct tggcccgtca tttagcgcta
    30661 tgctaatgca gaatttctcc gataatttat tgtttatcat gatcgccagc gtatcgttta
    30721 tctatttgct gatgctgctt cgcaacgccg gtcatacgcc gaaacccgtt gctcacgtgt
    30781 aaatgaattc aagcaaagtg tgaacttact gtttcacact ctgctttttt gtttcttcta
    30841 tctgacttgc ttttttccaa attttattcg tttaaaaata aaatgtgcag caggttataa
    30901 ttttgcattt cgctatttcc gcacttctta tttgccgcgc ataatccctc gttttacaga
    30961 tgccccttta attttggcga aggatttgtc tatggctggg aatgttcag
//
LOCUS       DAOFMX010000049        30666 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.049, whole genome shotgun sequence.
ACCESSION   DAOFMX010000049 DAOFMX010000000
VERSION     DAOFMX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.049"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..588
                     /gene="tuf"
                     /locus_tag="QBA20_002454"
     CDS             <1..588
                     /gene="tuf"
                     /locus_tag="QBA20_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDQ6292953.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="QBA20_002455"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="QBA20_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDQ6292954.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="QBA20_002456"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="QBA20_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDQ6292955.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="QBA20_002457"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="QBA20_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDQ6292956.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="QBA20_002458"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="QBA20_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDQ6292957.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="QBA20_002459"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="QBA20_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDQ6292958.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="QBA20_002460"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="QBA20_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDQ6292959.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="QBA20_002461"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="QBA20_002461"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDQ6292960.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="QBA20_002462"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="QBA20_002462"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDQ6292961.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKARITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="QBA20_002463"
     CDS             complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="QBA20_002463"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDQ6292962.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="QBA20_002464"
     CDS             complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="QBA20_002464"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDQ6292963.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="QBA20_002465"
     CDS             complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="QBA20_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDQ6292964.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="QBA20_002466"
     CDS             complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="QBA20_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDQ6292965.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="QBA20_002467"
     CDS             complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="QBA20_002467"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDQ6292966.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="QBA20_002468"
     CDS             complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="QBA20_002468"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDQ6292967.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18720..18820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="QBA20_002469"
     CDS             complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="QBA20_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDQ6292968.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLIDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19444..20217
                     /gene="nudC"
                     /locus_tag="QBA20_002470"
     CDS             19444..20217
                     /gene="nudC"
                     /locus_tag="QBA20_002470"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDQ6292969.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20257..21321
                     /gene="hemE"
                     /locus_tag="QBA20_002471"
     CDS             20257..21321
                     /gene="hemE"
                     /locus_tag="QBA20_002471"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDQ6292970.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21478..22149
                     /gene="nfi"
                     /locus_tag="QBA20_002472"
     CDS             21478..22149
                     /gene="nfi"
                     /locus_tag="QBA20_002472"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDQ6292971.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22192..22782
                     /locus_tag="QBA20_002473"
     CDS             22192..22782
                     /locus_tag="QBA20_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDQ6292972.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22969..23241
                     /gene="hupA"
                     /locus_tag="QBA20_002474"
     CDS             22969..23241
                     /gene="hupA"
                     /locus_tag="QBA20_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDQ6292973.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23254..23949
                     /locus_tag="QBA20_002475"
     CDS             23254..23949
                     /locus_tag="QBA20_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDQ6292974.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWMEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="QBA20_002476"
     CDS             complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="QBA20_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDQ6292975.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24614..25990
                     /gene="zraS"
                     /locus_tag="QBA20_002477"
     CDS             24614..25990
                     /gene="zraS"
                     /locus_tag="QBA20_002477"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDQ6292976.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25987..27312
                     /gene="zraR"
                     /locus_tag="QBA20_002478"
     CDS             25987..27312
                     /gene="zraR"
                     /locus_tag="QBA20_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDQ6292977.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27309..28598)
                     /gene="purD"
                     /locus_tag="QBA20_002479"
     CDS             complement(27309..28598)
                     /gene="purD"
                     /locus_tag="QBA20_002479"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDQ6292978.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28610..30199)
                     /gene="purH"
                     /locus_tag="QBA20_002480"
     CDS             complement(28610..30199)
                     /gene="purH"
                     /locus_tag="QBA20_002480"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDQ6292979.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7193 a         7983 c         8439 g         7051 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggg cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcatacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggc
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cattgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggcagg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgtgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgt ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccaa gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaatatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagcgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggca tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcaaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag gggggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatattctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    11941 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacacct tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgtaaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13141 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13201 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13261 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13321 cataccggct gcaaaccctg agcggttaac acggcagcaa ccgcttccgg tctgcgatcg
    13381 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13441 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13501 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13561 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13621 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    13681 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    13741 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    13801 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    13861 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    13921 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    13981 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14041 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14101 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14161 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14221 cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc
    14281 atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt
    14341 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag
    14401 gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac
    14461 tgcggctgcc cggtccggac tgacgtgcca gcaggcctac ttctaccgcc agacgaaatg
    14521 ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt
    14581 cggcccccat ttccagcgcc tgcgtggcat ggctgggaac gccgatgcca gcatcgacaa
    14641 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    14701 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    14761 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    14821 gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    14881 gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    14941 tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga
    15001 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15061 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15121 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15181 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15241 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15301 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15361 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15421 caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc
    15481 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac
    15541 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag
    15601 gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc
    15661 gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg
    15721 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt
    15781 caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg
    15841 tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag
    15901 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    15961 gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16021 cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat
    16081 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16141 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16201 tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca
    16261 cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg
    16321 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16381 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16441 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16501 catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16561 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat
    16621 ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    16681 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    16741 acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga
    16801 catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct
    16861 gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga
    16921 ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    16981 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17041 cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag
    17101 cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca
    17161 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17221 atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17281 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc
    17341 ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg
    17401 caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17461 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17521 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17581 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    17641 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    17701 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    17761 tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    17821 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    17881 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    17941 caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18001 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18061 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18121 gcggcccata ttctcgcgga tggcgatgaa ttccatgtcc ggcgtgatga tgccctggcg
    18181 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18241 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18301 gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc
    18361 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18421 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18481 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18541 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18601 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag
    18661 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    18721 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    18781 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    18841 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18901 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    18961 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19021 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19081 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19141 aaatgtccgg cagacaagta atcgatcagg ctctgacaaa aatcatcaag ggctttttcg
    19201 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19261 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19321 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19381 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19441 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19501 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19561 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag
    19621 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19681 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19741 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg tagccattgc
    19801 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19861 tcgctcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19921 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19981 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20041 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20101 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20161 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20221 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20281 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20341 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20401 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20461 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20521 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20581 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20641 cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20701 tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa
    20761 aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc
    20821 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    20881 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20941 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21001 accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc
    21061 ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21121 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21181 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21241 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21301 tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag
    21361 gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc
    21421 cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg
    21481 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat
    21541 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    21601 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    21661 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    21721 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    21781 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta
    21841 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    21901 ctctccagca aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    21961 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    22021 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    22081 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    22141 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    22201 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    22261 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    22321 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    22381 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    22441 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg
    22501 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    22561 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatggg cgatgaagag
    22621 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    22681 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    22741 gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    22801 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    22861 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    22921 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    22981 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    23041 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    23101 gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc gtactggccg caacccgcag
    23161 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    23221 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    23281 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    23341 gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    23401 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    23461 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    23521 acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac
    23581 gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    23641 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    23701 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    23761 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    23821 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggatgga agcgcccgaa
    23881 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    23941 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    24001 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    24061 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    24121 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    24181 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    24241 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    24301 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    24361 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    24421 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    24481 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    24541 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    24601 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    24661 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    24721 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24781 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24841 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24901 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24961 ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    25021 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    25081 tggaatgtac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    25141 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    25201 tattctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    25261 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    25321 ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    25381 taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact
    25441 ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga
    25501 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    25561 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    25621 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    25681 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25741 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    25801 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    25861 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25921 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25981 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    26041 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    26101 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    26161 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    26221 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    26281 ggggcgcagg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    26341 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    26401 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    26461 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    26521 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    26581 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    26641 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    26701 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    26761 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    26821 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26881 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26941 atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    27001 aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt
    27061 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    27121 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat
    27181 attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    27241 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa
    27301 ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag
    27361 caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg
    27421 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca
    27481 tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg
    27541 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    27601 ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc
    27661 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27721 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27781 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27841 cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag
    27901 taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc
    27961 tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    28021 tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc
    28081 gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct
    28141 ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    28201 accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg
    28261 gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg
    28321 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    28381 tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg
    28441 atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta
    28501 cccggagcaa caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    28561 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    28621 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    28681 cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg
    28741 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28801 ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28861 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca
    28921 cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac
    28981 gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca
    29041 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc
    29101 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    29161 aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat
    29221 ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    29281 cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct
    29341 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    29401 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg
    29461 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    29521 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    29581 ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt
    29641 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    29701 gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29761 cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat
    29821 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg
    29881 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29941 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    30001 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    30061 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    30121 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    30181 ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct
    30241 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    30301 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    30361 ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    30421 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30481 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgccttca tcgacacggc
    30541 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca
    30601 gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg cgctaaagcg
    30661 cgtcgc
//
LOCUS       DAOFMX010000050        29903 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.050, whole genome shotgun sequence.
ACCESSION   DAOFMX010000050 DAOFMX010000000
VERSION     DAOFMX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.050"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            57..851
                     /locus_tag="QBA20_002481"
     CDS             57..851
                     /locus_tag="QBA20_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDQ6292980.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            873..2282
                     /locus_tag="QBA20_002482"
     CDS             873..2282
                     /locus_tag="QBA20_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6292981.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVVAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="QBA20_002483"
     CDS             2260..3039
                     /locus_tag="QBA20_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDQ6292982.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="QBA20_002484"
     CDS             3036..3896
                     /locus_tag="QBA20_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDQ6292983.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="QBA20_002485"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="QBA20_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="QBA20_002486"
     CDS             complement(4636..4896)
                     /locus_tag="QBA20_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDQ6292984.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="QBA20_002487"
     CDS             complement(4887..6158)
                     /locus_tag="QBA20_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDQ6292985.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="QBA20_002488"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="QBA20_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDQ6292986.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="QBA20_002489"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="QBA20_002489"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDQ6292987.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTQAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="QBA20_002490"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="QBA20_002490"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDQ6292988.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="QBA20_002491"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="QBA20_002491"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDQ6292989.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="QBA20_002492"
     CDS             11500..11652
                     /locus_tag="QBA20_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDQ6292990.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11846..12937
                     /locus_tag="QBA20_002493"
                     /pseudo
     CDS             11846..12937
                     /locus_tag="QBA20_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13083..14120)
                     /gene="iap"
                     /locus_tag="QBA20_002494"
     CDS             complement(13083..14120)
                     /gene="iap"
                     /locus_tag="QBA20_002494"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDQ6292991.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14372..15280
                     /gene="cysD"
                     /locus_tag="QBA20_002495"
     CDS             14372..15280
                     /gene="cysD"
                     /locus_tag="QBA20_002495"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDQ6292992.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15282..16709
                     /gene="cysN"
                     /locus_tag="QBA20_002496"
     CDS             15282..16709
                     /gene="cysN"
                     /locus_tag="QBA20_002496"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDQ6292993.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16709..17314
                     /gene="cysC"
                     /locus_tag="QBA20_002497"
     CDS             16709..17314
                     /gene="cysC"
                     /locus_tag="QBA20_002497"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDQ6292994.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17364..17687
                     /locus_tag="QBA20_002498"
     CDS             17364..17687
                     /locus_tag="QBA20_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDQ6292995.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17881..18192
                     /gene="ftsB"
                     /locus_tag="QBA20_002499"
     CDS             17881..18192
                     /gene="ftsB"
                     /locus_tag="QBA20_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDQ6292996.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18211..18921
                     /gene="ispD"
                     /locus_tag="QBA20_002500"
     CDS             18211..18921
                     /gene="ispD"
                     /locus_tag="QBA20_002500"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDQ6292997.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18921..19400
                     /gene="ispF"
                     /locus_tag="QBA20_002501"
     CDS             18921..19400
                     /gene="ispF"
                     /locus_tag="QBA20_002501"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDQ6292998.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19397..20446
                     /gene="truD"
                     /locus_tag="QBA20_002502"
     CDS             19397..20446
                     /gene="truD"
                     /locus_tag="QBA20_002502"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDQ6292999.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20427..21188
                     /gene="surE"
                     /locus_tag="QBA20_002503"
     CDS             20427..21188
                     /gene="surE"
                     /locus_tag="QBA20_002503"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDQ6293000.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21182..21808
                     /gene="pcm"
                     /locus_tag="QBA20_002504"
     CDS             21182..21808
                     /gene="pcm"
                     /locus_tag="QBA20_002504"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDQ6293001.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21948..23087
                     /gene="nlpD"
                     /locus_tag="QBA20_002505"
     CDS             21948..23087
                     /gene="nlpD"
                     /locus_tag="QBA20_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDQ6293002.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23150..24142
                     /gene="rpoS"
                     /locus_tag="QBA20_002506"
     CDS             23150..24142
                     /gene="rpoS"
                     /locus_tag="QBA20_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDQ6293003.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24262..24669)
                     /locus_tag="QBA20_002507"
     CDS             complement(24262..24669)
                     /locus_tag="QBA20_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDQ6293004.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24816..25409
                     /locus_tag="QBA20_002508"
     CDS             24816..25409
                     /locus_tag="QBA20_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDQ6293005.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25409..26356
                     /locus_tag="QBA20_002509"
                     /pseudo
     CDS             25409..26356
                     /locus_tag="QBA20_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26629..27192)
                     /locus_tag="QBA20_002510"
     CDS             complement(26629..27192)
                     /locus_tag="QBA20_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293006.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27357..27608
                     /locus_tag="QBA20_002511"
     CDS             27357..27608
                     /locus_tag="QBA20_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6293007.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27610..29706
                     /locus_tag="QBA20_002512"
     CDS             27610..29706
                     /locus_tag="QBA20_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6293008.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7324 a         7404 c         8082 g         7093 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggcgtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggtggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggctg ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc agccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacactcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc caagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcattcttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cttggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc ctttttttaa atttatattt tttatgtgaa tatatagtct ctatttatta
    11761 ttatgaattt caaatgaaaa tcattaattt attcataatg taaatgtttt attggctgat
    11821 atatatcata gatagttttt aaaatatgcg taaatatcct ttaagtttac tgaaggataa
    11881 aaatattgtg actttctttg atttctgggg aaaaaaccga cgtggcgaga aagagggtat
    11941 cgacgactat caccttcttt gctggcattt gctggatgtg gccgcaatgg gctatttaat
    12001 ggttaaaaga aattgcttcg ggctggctga ttattttcgt caattaggga tttctgacaa
    12061 ggaacaggca gctcaatttt tcgcctggtt gctgtgctgg cacgatattg gaaaatttgc
    12121 ccgctctttt caacaacttt acctgccccc tgaattcaag attcaggaag gtgccagaaa
    12181 aaattacgaa aaaatctctc attcaacgct gggctactgg ttgtggaatc attatttaag
    12241 tgaatgtcag gagttacttc cttcatcttc gctctctccg cgcaaactta ggcgagtgat
    12301 agaaatgtgg atgcccgtga cgacagggca tcatggtcgg ccccctgatc gtatgaatga
    12361 attggataat tttctgcctg aagacaaagc tgcagcacga gattttctcc ttgaaatcaa
    12421 gccactgttt ccgcgaatag agatccccac attctgggat gatgacgagg gcgttgaact
    12481 tataaaacaa ctttcctggt atatctctgc aaccgtcgta cttgcagact ggacagggtc
    12541 gtcaacgcga ttttttccac gcgtcgcaca ggcaatggat attgaagatt actggcaaaa
    12601 ggctttagtt caggctcaaa acgcattaac cgtctttccg ccacaagcag aaaccgcgcc
    12661 tttcaccgga attaatacgc tgtttccttt tattgagaat acgacaccgt tacagcaaaa
    12721 ggtgctggat ctggatatca atcagccagg accacagttg tttattctgg aagatgtgac
    12781 cggcgcaggt aaaacagaag cggcgcttat cctggcgcac aggttgatgg ctgcgggtaa
    12841 cgctcagggg ctgttttttg gcctgccaac aatggcaacg gccaatgcca tgtacgatcg
    12901 actggtcaaa acctggcttg ctttctattc gccagaggtg ttccccgctc cagcggagat
    12961 aaaccggcgc aatacggtgc ggttttcccg gaagtaaacc ggacgcactg gatgcgatga
    13021 tgggtatcac ttagaattcc ccgcccctgc ggtagaactc ccagctccca ttttcaaacc
    13081 catcaagacg ccttcgccag ctccttcacc aacggtagca ttatccgcat aacgtcacgg
    13141 cagcgacgtt ctattcttcc aggaagtgcc ttatcaatat gttgttgatt atccagtctt
    13201 acgtcatgcc agctatttcc cgccgggaag gcaggtgttt ttgcgcgttg ctgataacca
    13261 tccttatttc caagattcca gttagtcgct tccaccgaaa gtacagcaat gcccgctttg
    13321 tcgaatattt ctgcgtcatt acaacaccca gtgcctttcg gataattttt attcaaaccc
    13381 ggattggtcg ttgcggctat tccgtgactg cgtgcaattg ccagcgccct gtcgcgcgtt
    13441 aatttcctta ctgcttcagg ggtttttaca ccgctgttga aatacaattt atcgccaaca
    13501 attaagttat cgagattaat caccagcagc gtatttttct tttcggtgtc actcatccgc
    13561 ttgagtaaat tctcagcgcc taattttcct tcctcttcgc cgctggtcgc cacaaatcga
    13621 ataccatact cggtaggcgt atttttcagg cgttctgcca gttccagcat gacacctaaa
    13681 cctgcggcgt tatcatctat tccttgtaac gtcagtccgc cgagattggc atctgcgtca
    13741 gcatcgctca gcggggcgta agtatccaga tgcgccataa tgatgatctg ctgcggcgct
    13801 ttgccttcat gagcggcaat caccgtactt cctgtcacgt tatgccagtt cttgcgatta
    13861 tcgcgggcgg tataaatata ccgactatta aatgtccgaa tatcactgcg ataacccatt
    13921 tgctgaaact gttggcgaat ataatcggca gataacattt ctgctggagt tccggtcatg
    13981 cgtcccggaa agaaagtagc aatatgtcgt gcctgggtat tagcaaaatc gccaggttta
    14041 ggtgacgagg cgtgtacggg gagaataaag catacgccga gcgccagggc agcggtacgg
    14101 tggcgcaatg cggaaaacat agtgagtcct taaataccat gcaaattttt ttaccgccat
    14161 agtatgaaac tgccgctgcg ctaaaacaat ttcaaatctt cctaaacgcc cgaaatccgg
    14221 tgccttaagc actttttgat attagctttg ccaaatcgtt attccgttaa ggaactactc
    14281 attctaattg gtaatttcat tcgttctctt acgctcccta tagtcgaaac atctgatggc
    14341 aagaaaatag cggtattgca aaggaacggt tatggatcaa aaacgactta ctcacctgcg
    14401 gcaactggag gcggaaagca tccacattat tcgcgaggtg gcggcagaat tctcaaatcc
    14461 ggtgatgctc tactctatcg gtaaagattc cagcgtcatg ctgcatctgg cgcgcaaggc
    14521 gttttatcca ggtacgctgc ctttcccgtt gctgcatgtc gataccggct ggaaattccg
    14581 cgagatgtat gagttccgcg atcgtacggc gaaagcctac ggctgcgagc tgctggtgca
    14641 taaaaacccg gaaggcgtgg cgatggggat taatccattc gtgcacggca gcgcgaaaca
    14701 taccgatatt atgaaaactg aaggcctgaa acaggcgctg aacaaatacg gttttgatgc
    14761 cgccttcggt ggtgcgcgcc gtgacgaaga gaaatcccgc gctaaagagc gaatctactc
    14821 tttccgtgac cgcttccatc gctgggatcc gaaaaatcag cgcccggagc tgtggcacaa
    14881 ctacaacggg caaattaaca aaggcgaaag catccgcgtc ttcccgctct ctaactggac
    14941 cgagcaggat atctggcaat acatctggct ggaaaatatc gacattgtgc cgctgtatct
    15001 cgctgcggaa cgtccggttc tggaacgcga cggtatgttg atgatgattg atgacaaccg
    15061 tatcgacctg caaccgggcg aagtgatcaa aaaacggatg gtgcgtttcc gtacgctggg
    15121 ctgctggccg ctgaccggtg cggtggagtc aaatgcacaa acactgccgg aaatcatcga
    15181 agagatgctg gtttccacca ccagtgaacg tcagggtcgc gtgattgacc gcgaccaggc
    15241 gggatccatg gagctgaaaa aacgtcaggg gtatttttaa gatgaacacc gtacttgcac
    15301 aacaaatcgc caatgaaggc ggcgtcgaag cctggatgat tgcgcaacaa cataaaagcc
    15361 tgctgcgttt tctgacctgt ggtagcgtcg atgacggcaa aagtaccctg attggtcgtc
    15421 tgctgcacga tacccgccaa atctacgaag atcagctctc atcgctgcat aacgacagta
    15481 agcgtcacgg cacccagggc gaaaagctgg atctggctct gctggtggac ggcctgcaag
    15541 ctgagcgcga acagggcatc actattgatg tggcctaccg ctatttctct accgagaagc
    15601 gtaaatttat tatcgccgac accccagggc acgagcagta cactcgcaat atggcgactg
    15661 gcgcatcgac atgtgaactg gcgatcttac tgattgatgc ccgtaaaggc gtgctcgatc
    15721 aaacccgtcg tcacagtttt atctccacac tgttggggat caaacatctg gtcgtggcga
    15781 tcaacaaaat ggatctggtg gattacagtg aagagacgtt cacccgtatt cgtgaagatt
    15841 atctgacctt tgccgggcag ctgccggata atctggatat ccgctttgtg ccgctctccg
    15901 cactggaagg tgacaacgtg gcttcgcaaa gtgaaagtat gccgtggtac agcggtctga
    15961 cactgctcga agtgctggaa accgtggaga tccagcgagt ggtggatgcc cagccaatgc
    16021 gcttcccggt gcagtacgtt aaccgcccaa atctcgattt tcgtggctac gccggaatgc
    16081 tggcatccgg tcgcgtggaa gtcgggcaac gtgtaaaagt gctgccctct ggtgtggaat
    16141 caaacgtcgc gcggatcgtg acttttgatg gtgatcgcga agaagtcttt gccggagaag
    16201 cgatcaccct ggtgctgacg gatgagatcg atatcagccg tggtgatctg ctgctggcgg
    16261 cagacgaagc gttaccggcg gtgcagagtg cgtcggtgga tgtggtatgg atggcggaac
    16321 agccgctttc cccgggccag agttatgaca tcaaaattgc cggtaagaag acgcgtgctc
    16381 gtgttgatgg cattcgttat caggttgata ttaataacct tacccaacgc gaagttgaaa
    16441 acctgccgct gaacggcatc ggcctggtgg atctcacttt tgacgagcca ctggtgttag
    16501 atcgctatca gcaaaatccg gttaccggtg ggctgatttt tatcgatcgc ctgagcaatg
    16561 tgaccgtggg tgccggtatg gtgcacgagc cagttagcca ggcaacagct gcgtcatctg
    16621 aattcagtgc attcgaactg gaattgaatg cactggttcg tcgccacttt ccgcactggg
    16681 gcgcgcgcga tttgctgggg gataaataat ggcgctgcat gacgaaaacg tcgtctggca
    16741 tagtcatccg gtcactgtgc aacaacgcga actacaccac ggtcatcgtg gtgtagtgct
    16801 gtggtttacc ggcctctccg ggtccggtaa atcaacggtc gccggggcgc tggaggaggc
    16861 gttacataaa ctcagcgtca gtacgtatct gctggatggc gacaatgttc gccacggatt
    16921 atgcagcgat ctcggtttta gcgatgccga tcgtaaagag aatatccgtc gcgtcggtga
    16981 agtggcgaat ttgatggttg aagccggact ggtggtgctg accgcattta tctcgccaca
    17041 ccgcgccgaa cgccagatgg ttcgcgaacg cgtaggagaa gggcgcttta ttgaagtgtt
    17101 tgtcgatacg ccgctggcga tttgcgaagc ccgcgatcca aaaggcttat ataagaaagc
    17161 gcgtgccggt gaactgcgca actttacggg aatagattcc gtttacgaag cgcctgaatc
    17221 ggcagaaatt catctcaatg gtgaacaatt agtaacaaat ttggtacagc aattattaga
    17281 tctgttgaga cagaacgata ttatcagatc ctgagacacc accgggtttc catgcccggt
    17341 tcgtcaaagt aacaggatca aatatgcgta atagccataa cattacacta acaaataacg
    17401 acagccttac tgaggatgaa gaaactacat ggtcactgcc tggtgccgtg gtcggtttta
    17461 tctcctggtt atttgcgctg gcgatgccga tgttgattta tggctctaac acgctgttct
    17521 tctttctcta cacctggcct ttctttctgg cactgatgcc tgtcgcggta gtggtgggga
    17581 ttgcgctgca ttcattgatg gacggaaagc tacgctacag tattgttttc accctggtga
    17641 cagtaggcat tatgtttggc gcactgttta tgtggctact gggctaattt gtactttctt
    17701 gtctcctctg ttcataattc aaaccgtaac taataatgag attatgttct gcacgccctg
    17761 ggtatacgta acaatggaca aatgtggtac atttgcccgc gttgtcgcgg tatccccaac
    17821 agaggatgta gagtcgtctt cggatgcatg ggatgatgat gcagtttttc agggggcagg
    17881 atgggtaaac taacgctgct gttgctggct attctggtct ggctacagta ttcgctgtgg
    17941 ttcggtaaga acggtataca tgactatacc cgcgtcaatg atgatgtggc ggcactgcaa
    18001 gctacaaacg cgaaacttaa agcgcgaaac gatcaacttt ttgccgaaat tgacgatctc
    18061 aatggcggcc aggaggcgct cgaagagcgt gcgcgtaatg aactcagcat gaccaggccg
    18121 ggcgaaactt tttatcgtct ggtgcctgac gcgtcgaagc gcgcacagtc tgcggggcaa
    18181 aacaatcgat aaatcagccc gggaattaac atggcaacca ctcatttgga tgtttgcgcc
    18241 gtggttccgg cggccggatt tggccgtcga atgcaaacgg aatgtcctaa gcaatatctc
    18301 tcaatcggta atcaaaccat tcttgaacac tcggtgcatg cgctgctggc gcatccccgg
    18361 gtgacacgtg tcgtcattgc cataagtcct ggcgatagcc gttttgcaca acttcctctg
    18421 gcgaatcatc cgcaaatcac cgttgtagat ggcggtgatg agcgtgccga ttccgtgctg
    18481 gcaggtctga aagccgctgg cgatgtgcag tgggtattgg tgcatgacgc cgctcgtcct
    18541 tgtttgcatc aggatgacct cgcgcgattg ttggcgttga gcgaaaccag ccgcacggga
    18601 gggatcctcg ccgcaccagt gcgcgatact atgaaacgtg ccgaaccggg caaaaatgcc
    18661 attgctcata ccgttgatcg caacggctta tggcacgcgc tgacgccgca atttttccct
    18721 cgtgagctgt tacatgactg tctgacgcgc gctctaaatg aaggcgctac cattaccgac
    18781 gaagcctcgg cgctggaata ttgcggattt catccgcagt tggtcgaagg ccgcgcggat
    18841 aacattaaag tcacgcgccc ggaagatttg gcactggccg agttttacct cacccgaacc
    18901 atccatcagg agaatacata atgcgaattg gacacggttt tgacgtacac gcctttggcg
    18961 gtgaaggccc aattatcatt ggtggcgtac gcattcctta cgaaaaagga ttgctggcgc
    19021 attctgatgg cgacgtggcg cttcatgcgt tgaccgatgc attgcttggc gcggcggcgc
    19081 tgggggatat cggcaaactg ttcccggata ccgatccggc atttaaaggt gccgacagcc
    19141 gcgagctgct acgcgaagcc tggcgtcgta ttcaggcgaa gggttatacc cttggcaacg
    19201 tcgatgtcac tatcatcgct caggcaccga agatgttgcc gcacattcca caaatgcgcg
    19261 tgtttattgc cgaagatctc ggctgccata tggatgatgt taacgtgaaa gccactacta
    19321 cggaaaaact tggatttacc ggacgtgggg aagggattgc ctgtgaagcg gtggcgctac
    19381 tcattaaggc aacaaaatga ttgagtttga taatctcact tacctccacg gtaaaccgca
    19441 aggcaccggg ctgctgaaag ccaatccgga agactttgtg gtggtggaag atttgggctt
    19501 tgagcctgat ggtgaaggtg agcatattct ggttagaatc ctcaaaaacg gctgcaatac
    19561 ccgttttgtg gcggatgcac tggcgaaatt cctgaaaatt cattcccgtg aagtcagctt
    19621 cgctgggcaa aaagacaaac atgctgtaac ggaacagtgg ttatgtgctc gcgtgccggg
    19681 caaggaaatg cccgatctga gcgcctttca actggaaggc tgccaggtgc tggagtatgc
    19741 gcggcacaag cgcaagctgc gtttaggggc gctgaaaggt aacgccttta ccctggttct
    19801 gcgcgaagtg agcaatcgcg atgacgttga acaacgtctg atcgatattt gcgtaaaagg
    19861 tgtaccgaac tacttcggtg cccaacgttt tgggattggc ggtagcaact tgcagggcgc
    19921 gctgcgctgg gcgcaaacca atactccggt gcgcgatcgc aataaacgga gtttttggtt
    19981 gtcggcagcc cgcagtgcgt tgtttaatca gattgttgct gagcgcctca aaaaagcaga
    20041 cgttaatcaa gttgttgacg gcgatgcgct acaattagcc ggacgtggta gctggtttgt
    20101 agcaaccacc gaagaactgg cggaattaca gcgtcgcgtc aacgataaag agctgatgat
    20161 aaccgccgca ttgccgggca gtggcgaatg gggaactcag cgtgaagcgc tggcattcga
    20221 acaagcagct gtcgccgcag aaactgaatt acaagcttta ctggtgcgcg aaaaagttga
    20281 agccgcgcgc agagcgatgc tgctgtatcc gcaacaatta agctggaact ggtgggatga
    20341 cgtcaccgta gagatccgtt tctggcttcc ggcgggtagt tttgcaacca gcgttgtcag
    20401 ggaacttatc aacacaacag gtgattatgc gcatattgct gagtaatgat gacggggtac
    20461 atgcacccgg tatacaaacg ctggcgaaag ccttgcgtga gtttgctgac gttcaggtgg
    20521 tcgcccccga tcgtaaccgc agcggcgctt caaattctct gacactggaa tcctccctgc
    20581 gcacgtttac ctttgaaaat ggtgatattg ctgtgcaaat gggaaccccg accgattgtg
    20641 tctatcttgg cgtgaatgct ctgatgcgtc cgcgcccgga cattgttgtg tccggaatta
    20701 acgccgggcc gaatctgggg gatgatgtta tttattccgg tacggtagcc gccgcgatgg
    20761 aaggccgtca tttaggtttt ccggcgctgg ccgtctcgct tgacgggcat aaacattacg
    20821 acactgccgc ggcggtaacc tgttcaattt tgcgcgcact gtgtaaagag ccgctgcgca
    20881 ccggacgtat tcttaatatt aacgttcagg atttaccctt ggatcaaatc aaaggtattc
    20941 gcgtgacgcg ctgcggtaca cgacatccgg cagatcaggt gatcccgcag caagatccgc
    21001 gcggcaatac gctgtactgg attggcccgc cgggcggtaa atgtgatgcc ggtccgggga
    21061 ccgattttgc tgcggtagat gagggctatg tctccatcac gccgctgcat gtggatttaa
    21121 ctgcgcatag cgcgcaagat gtggtttcag actggttaaa cagcgtggga gttggcacgc
    21181 aatggtaagc agacgcgtac aagcacttct ggatcaatta cgtgcgcaag gtattcagga
    21241 tgagcaggtg ctgaatgcac ttgccgccgt gccgcgtgaa aaattcgttg atgaagcgtt
    21301 tgaacaaaaa gcctgggaca atatcgcctt gccgataggt caggggcaga caatttcgca
    21361 gccgtatatg gtggcgcgaa tgaccgaatt actcgagctg acgccgcagt cgcgggtgct
    21421 ggaaattggc actggttcgg gatatcaaac ggcaatcctg gcgcatcttg tccagcatgt
    21481 ttgctcggtt gaacggatta aaggcttgca gtggcaggca cgtcgccgcc tgaaaaatct
    21541 tgatttacat aatgtttcaa cccgtcatgg tgatggatgg caaggttggc aggcacgtgc
    21601 gccgtttgac gctatcattg ttacggcggc accgccggaa attccaactg cgctaatgac
    21661 gcagctggac gaaggcggga ttctcgtctt acccgtaggg gaggagcacc agtatttgaa
    21721 acgggtgcgt cgtcggggag gcgaatttat tatcgatacc gtggaggccg tgcgctttgt
    21781 ccctttagtg aagggtgagc tggcttaaaa cgtgaggaaa tacctggatt tttcctggtt
    21841 attttgccgt aggtcagcgt atcgtgaaca tcttttccag tgttcagtag ggtgccttgc
    21901 acggtaatta tgtcactggt tattaaccaa tttttcctgg gggataaatg agcgcgggaa
    21961 gcccaaaatt caccgttcgc cgcattgcgg ctttgtcact ggtttcgcta tggctggcag
    22021 gctgttctga cacttcaaat ccaccggcac cggtcagctc cgttaatggc aatgcgcctg
    22081 caaatactaa ttctggtatg ttgattacgc cgccgccgaa aatggggacg acgtctacag
    22141 cgcagcaacc gcaaattcag ccggtacagc agccacaaat tcaggctact caacaaccgc
    22201 aaatccagcc agtgcagcca gtagctcagc agccggtaca gatggaaaac ggacgcatcg
    22261 tctataaccg tcagtatggg aacattccga aaggcagtta tagcggcagt acctataccg
    22321 tgaaaaaagg cgacacactt ttctatatcg cctggattac tggcaacgat ttccgtgacc
    22381 ttgctcagcg caacaatatt caggcaccat acgcgctgaa cgttggtcag accttgcagg
    22441 tgggtaatgc ttccggtacg ccaatcactg gcggaaatgc cattacccag gccgacgcag
    22501 cagagcaagg agttgtgatc aagcctgcac aaaattccac cgttgctgtt gcgtcgcaac
    22561 cgacaattac gtattctgag tcttcgggtg aacagagtgc taacaaaatg ttgccgaaca
    22621 acaagccaac tgcgaccacg gtcacagcgc ctgtaacggt accaacagca agcacaaccg
    22681 agccgactgt cagcagtaca tcaaccagta cgcctatctc cacctggcgc tggccgactg
    22741 agggcaaagt gatcgaaacc tttggcgctt ctgagggggg caacaagggg attgatatcg
    22801 caggcagcaa aggacaggca attatcgcga ccgcagatgg ccgcgttgtt tatgctggta
    22861 acgcgctgcg cggctacggt aatctgatta tcatcaaaca taatgatgat tacctgagtg
    22921 cctacgccca taacgacaca atgctggtcc gggaacaaca agaagtgaag gcggggcaaa
    22981 aaatagcaac catgggtagc accggaacca gttcaacacg cttgcatttt gaaattcgtt
    23041 acaaggggaa atccgtaaac ccgctgcgtt atttgccgca gcgataaatc ggcggaacta
    23101 ggcttttgct tgaatgttcc gtcaagggat cacgggtagg agccacctta tgagtcagaa
    23161 tacgctgaaa gttcatgatt taaatgaaga tgcggaattt gatgagaacg gagttgaggt
    23221 ttttgacgaa aaggccttag tagaagagga acccagtgat aacgatttgg ccgaagagga
    23281 actgttatcg cagggagcca cacagcgtgt gttggacgcg actcagcttt accttggtga
    23341 gattggttat tcaccactgt taacggccga agaagaagtt tattttgcgc gtcgcgcact
    23401 gcgtggagat gtcgcctctc gccgccggat gatcgagagt aacttgcgtc tggtggtaaa
    23461 aattgcccgc cgttatggca atcgtggtct ggcgttgctg gaccttatcg aagagggcaa
    23521 cttggggctg atccgcgcgg tagagaagtt tgacccggaa cgtggtttcc gcttctcaac
    23581 atacgcaacc tggtggattc gccagacgat tgaacgggcg attatgaacc aaacccgtac
    23641 tattcgtttg ccgattcaca tcgtaaagga gctgaacgtt tacctgcgaa ccgcacgtga
    23701 gttgtcccat aagctggacc atgaaccaag tgcggaagag atcgcagagc aactggataa
    23761 gccagttgat gacgtcagcc gtatgcttcg tcttaacgag cgcattacct cggtagacac
    23821 cccgctgggt ggtgattccg aaaaagcgtt gctggacatc ctggccgatg aaaaagagaa
    23881 cggtccggaa gataccacgc aagatgacga tatgaagcag agcatcgtca aatggctgtt
    23941 cgagctgaac gccaaacagc gtgaagtact ggcacgtcga ttcggtttgc tggggtatga
    24001 agcggcaaca ctggaagatg taggtcgtga aattggcctc acccgtgaac gtgttcgcca
    24061 gattcaggtt gaaggcctgc gccgtttgcg cgaaatcctg caaacgcagg ggctgaatat
    24121 cgaagcgctg ttccgcaagt aagtaagcat ctgtcagaaa ggccagtctc aagcgaggct
    24181 ggcctttttc ttttgtttgg gaagcttgtg tttttgtcgc ctggataaga cacgtcagcg
    24241 tcctacccgg caatcagcat tctatgtgct catcatcttg cgcaccaact gcataaacag
    24301 ctcctgctct tctccactca aacgccccaa aaattcctca tccacgctgt caccaatagg
    24361 tatcgccgca gcaagtagct tttctccttc ggcagtcagc cagacaaagc gtcgccgctt
    24421 gtcagcgacg tcatgttcac gtctgactaa gccacgattt tccattcttg ccaacatttc
    24481 tgctagcgtt gctttggtgc tgactgctgc ttctatcagt gccacctgtt caataccagg
    24541 tttatcagca atggcgcgca tcaccgcgta ctgtggtttg gtcaggtcag gtaactcgtg
    24601 ctgccagcga gccgtgtgct gctgaaaaag ctgtcgtaac tgatggaacg ctttatttcg
    24661 taacgccatg taaactccgg gctaattctc tgtcgctatc atagcggttt tttagtgacg
    24721 aagaagatgt tgttactttt caatccttgc cgctgtggaa atgaaggagt atgttaataa
    24781 taacgttcgc atacgaataa ttaagagaat aagcaatgaa actgatcgtc gggatgacag
    24841 gggctaccgg tgcgcctctt ggtgtggcat tactgcaagc gctgcgtgag atgccgaatg
    24901 tcgagactca tctggtgatg tcgaagtggg cgaaaaccac cattgaactg gaaacgcctt
    24961 acagcgctcg cgatgttgct gccctcgcag acttcagcca taacccggcg gatcaggcgg
    25021 cgatcatctc atccggttct tttcgtaccg acggcatgat cgttattccg tgcagtatga
    25081 aaacgctcgc cggtatccgc gctggttacg ccgatggcct ggtagggcgc gcggcggacg
    25141 tcgtgctcaa agaaggccgc aaactggtgc tggtgccgcg tgaaatgccg cttagcacca
    25201 tccatctcga aaatatgctc gcactttcac gcatgggcgt ggcgatggtg ccgccgatgc
    25261 ctgcctttta taaccatccc gaaacggtag atgacattgt ccaccatgtg gtagcccgcg
    25321 tgctggatca atttggcctc gaacatcccc acgccaggcg ctggcaagga ttaccgcagg
    25381 cccggaattt ttctcaggag aatgaataat ggcatttgat gatttacgca gctttttaca
    25441 ggcgcttgat gaccacggcc agttactgaa aatcagcgaa gaagtgaacg ctgagccgga
    25501 tctggcagca gcagctaacg ccaccgggcg tatcggcgac ggcgcgcccg cgctgtggtt
    25561 tgataatatt cgcggcttta ccgatgcccg cgtggcgatg aataccatcg gttcctggca
    25621 gaaccacgcg atttcccttg gcctgccacc aaacaccccg gtcaaaaagc agattgatga
    25681 gtttatccgc cgctgggata acttcccgat tgccccggag cgccgcgcca atccagcctg
    25741 ggcgcagaac accgtcgatg gcgaagagat caacctgttc gatattctgc cgctgtttcg
    25801 cctgaatgat ggcgatggcg gtttctatct cgacaaagcg tgcgtggttt cccgcgatcc
    25861 gctcgacccg gataacttcg gcaagcagaa cgtcggcatc taccgcatgg aagtgaaggg
    25921 caagcgtaag ctcggcctgc aaccggtgcc gatgcacgat atcgccctgc atctgcataa
    25981 agcagaagag cgcggtgaag atctgccgat tgcgatcacg ctcggtaacg atccgatcat
    26041 cacgctgatg ggggccacgc cgctgaaata cgatcagtct gagtatgaaa tggcaggcgc
    26101 gctgcgtgaa agcccgtacc cgatcgccac cgctccgctg accggttttg atgtgccgtg
    26161 gggttcagaa gtgatcctcg aaggggttat cgaaagccgt aaacgcgaaa tcgaagggcc
    26221 gttcggtgag tttaccgggc actactccgg tgggcgcaac atgaccgtgg tgcgcatcga
    26281 taaagtctct tatcgcagca aaccgatttt cgaatcactc tatctcggta tgccgtggac
    26341 cgaaatcgac tacctatgac taggtaattt attcaagata accgggaaga gattaaaaaa
    26401 gacgggaata gattggaatc aacaagttag gaactcaagg ttttcaagat aaagcgagtc
    26461 caaaaatgtt aaacggttcg atatcaataa aacattcaag ataactcaca ggttcttaca
    26521 atagccttta aagaccgttc aaaaacgatt caggtgttta ttatagcgct gatgggtgca
    26581 agcgccaaaa ccaatgtaat tttagcacag ctaggcactt gcactgggtt atgcgctggc
    26641 tgctttctga ggtagattta agtgatcgcc cttagcttcg gacaacgcgt aaatctctct
    26701 aaccttttcg tctggcaaat taccaaggct taacgctatg gctctacgaa ctccaactag
    26761 agcgtgcaac tcttggatgt gctcatccag aagaatgagt aattgctccg ctccttttct
    26821 cccaagaaca tcagctactt gttgtttttc ttgtggttta agaaatgaca agaacattag
    26881 aatttgagca tcaacattat ccgcattctc ttttttgggt tctttggaat cttttagttc
    26941 aacatcaccc tctcccgtca tgagccattc caaggtaaca ttttcgcttt ctgctatctt
    27001 ttttaactga tgcgggcgtg gtatcggtgc gtaatcccgc ctgttgtaat aattttttaa
    27061 agtgccttca ttgatccccc atgcccgagc agccgcattt cttgaagggt atcgttctac
    27121 taacctaaag attctttcga taatcggttc ttttccatcg gcaggaaaag aaccttgggg
    27181 tgcttttctc attcttggtt cctttctatc taattcattg ataaataata aaattaaaaa
    27241 ataaaccata aaaacaccaa agaacccttt acagggaatc tttggtatct taatctttat
    27301 ccatacggat atccgaccgg atatccgcgc gggtaatttt gtaggttaaa ggaaagatga
    27361 gacaaagaga agatagtgac cgcgattggc cttcgctgaa gattcagctg gcgctcaaag
    27421 ataacgggtg gtcttccttc cgggagcttt cactccatgc tggttatgct caggattcac
    27481 tgaaagcggt agttcgcaca ccaaacaaaa aatacgaacg cgtaatttct gacgctatag
    27541 gtgtaccgcc agaaatcatc tggcccagcc ggtatcgtaa cgatggattt atcaggaagg
    27601 cgtcgtgata tgcagtcgta cgttaccgta aatgacctgc tcggcgtacc aggaatgcca
    27661 gccaccacaa aaggcatccg acaggcgtta cagcgttttt ctcgtgatct tggtgacgtt
    27721 agtcgccgtc gggaaggcac aaaagccatt gagtaccaca ttgattgtct gcctgaaata
    27781 acacgaaagg cactgcgtga acgctatgtg gaacagctgg tggctacaga gaacaatgtt
    27841 tctgaagtga aagctgttac cagaaaaaca cgcaatcctg acgctgtcca ggcaatcgaa
    27901 gcatatcgcg gttctccaca actgatggaa gaacgcctga atgcgctgac cgaaaaccag
    27961 cgctgggtat ctgaagcaag agctgcgctg gtggtggaag tgctgaagct ggaaagcgcc
    28021 ggtaaccccg ggcgactgaa agccattaac tttcttgttg aaaaagcccg taaaggtgag
    28081 ctgccggagc gcctgcaaca ggccgcagtt aacgccaatg caaaacgtgg cgctaatcgt
    28141 actatcagcc gcgatccact ttaccagtgg gttctgaaat acaaccagtc gcagaacgcc
    28201 gccgaacggc tgctgttact ggcaccggga aaacgcgacg aaataaaacc ggaagaaatc
    28261 agctggctgc ctgaatttct ggcgcagtat cgccaggtga acggcagacc catgtcagaa
    28321 gcctacgagg attttgtcgc agaatggcag cgtcgccatg cggacgaacc gtacatgctg
    28381 gaggtgatgc cttcctatga tgttgtccgg tatgccatga aaaaactgcc ggaagtggtg
    28441 aaacaaaaag ggcgcgttac cggcagtgaa taccgtcagc ttgaaggatt cacccgccgc
    28501 gactggacag ctatgccggt gaattatgtc tggattggtg acggtcacgg tatgaagctg
    28561 aaatgtgcgc atccgatcca cgggcgtcca ttctcaccgg aagtcacgtt tgtgatcgat
    28621 ggtggaacac ggtttgttgt tggctggagt cttgaccttg ccgaaaacgt gttcgccgtt
    28681 gccggtgcga tacagcacgg cattcgcaac cacggaaaac cgttcctgta ttactcggat
    28741 aacggttccg gtgaaacggc tgacatgctg gacaaagagg tcgtgggtat tctgccacgc
    28801 ctggggatta aacaccctac cggtattgcc ggtaatccac agggacgcgg cattattgaa
    28861 cgcctgaacc ggacactgcc gatgcgtata gccaggaggt atcgcaccta ttttggcaag
    28921 ggtgctgacc gggaatcgtt gcgtgtactt aaccgtgatc tgcgttcagc gtttaacgcc
    28981 ctgcaacagg acaagcctct gaacgaccgc cagaaagcgg caatgcgcga gctgccatcg
    29041 tgggcggagc ttattgaggc tattcgtgaa ggcgtggaat ggtacaacaa ccgcccgcat
    29101 tctgagctgc cgatgaaacc gaacggacgg cattacagcc cgacagagtt cagaaaaaaa
    29161 cgccaggcag aagaagacac cgaaattgag tggctgagtg atcttgagct gcgcgacatg
    29221 ttccgcccaa tggtggaacg accggtaaga cgctgcgaaa ttcagtggct gaacaatatt
    29281 tactacgcgc cggagttgcg tgacgaacac ggtcgcaaag tgcttatcag ttatgacatt
    29341 catgacgccg agcgaattac ggtacgtcgc aaggacggca gctttatctg tgaggcgata
    29401 tggaacggca ataaacgcgc agcctttgct gtcagtgctg aatatcacaa acaacagcag
    29461 cgtattaaag gaatgcgcaa acgcgctgag gaaaaaattc gtgatgctga agatgaaggt
    29521 attcagattc tggaacacaa acaggctgaa ccctggctga gtaatgttta tcgcccggtt
    29581 ggtaatgtgg tggcagttca gcaaccggaa tatgaagaag agcatgacga agaatttgaa
    29641 cgcgatttcc ggctggggat gcaaaaactt tttgccatgc aggaagaaga tgatccgctg
    29701 gcctgaataa aaaaacagtc cgcgggaacg gactgttaaa taaaggaaac aaagctgatt
    29761 tgaaataacc gaggaaatac tatgactgat attgatgatg tttgaggtgt actggcaata
    29821 gcggacacta ccatttgttc tttttttaag cagccatctg atgatatttt tccctgaagg
    29881 ctgccgggga gatattcccc aga
//
LOCUS       DAOFMX010000051        29469 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.051, whole genome shotgun sequence.
ACCESSION   DAOFMX010000051 DAOFMX010000000
VERSION     DAOFMX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.051"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            71..352
                     /locus_tag="QBA20_002513"
     CDS             71..352
                     /locus_tag="QBA20_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDQ6293009.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..1394
                     /locus_tag="QBA20_002514"
                     /pseudo
     CDS             318..1394
                     /locus_tag="QBA20_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1787..2128)
                     /locus_tag="QBA20_002515"
     CDS             complement(1787..2128)
                     /locus_tag="QBA20_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDQ6293010.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2141..3013)
                     /gene="copD"
                     /locus_tag="QBA20_002516"
                     /pseudo
     CDS             complement(2141..3013)
                     /gene="copD"
                     /locus_tag="QBA20_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3017..3391)
                     /gene="yobA"
                     /locus_tag="QBA20_002517"
     CDS             complement(3017..3391)
                     /gene="yobA"
                     /locus_tag="QBA20_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDQ6293011.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3530..3760
                     /gene="holE"
                     /locus_tag="QBA20_002518"
     CDS             3530..3760
                     /gene="holE"
                     /locus_tag="QBA20_002518"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDQ6293012.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3862..4518
                     /locus_tag="QBA20_002519"
                     /pseudo
     CDS             3862..4518
                     /locus_tag="QBA20_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4542..5204
                     /gene="exoX"
                     /locus_tag="QBA20_002520"
     CDS             4542..5204
                     /gene="exoX"
                     /locus_tag="QBA20_002520"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDQ6293013.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5201..7261)
                     /gene="ptrB"
                     /locus_tag="QBA20_002521"
     CDS             complement(5201..7261)
                     /gene="ptrB"
                     /locus_tag="QBA20_002521"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDQ6293014.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7471..8130)
                     /locus_tag="QBA20_002522"
     CDS             complement(7471..8130)
                     /locus_tag="QBA20_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDQ6293015.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8457..8813)
                     /locus_tag="QBA20_002523"
     CDS             complement(8457..8813)
                     /locus_tag="QBA20_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDQ6293016.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8880..9170)
                     /gene="yebG"
                     /locus_tag="QBA20_002524"
                     /pseudo
     CDS             complement(8880..9170)
                     /gene="yebG"
                     /locus_tag="QBA20_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9304..10482
                     /gene="purT"
                     /locus_tag="QBA20_002525"
     CDS             9304..10482
                     /gene="purT"
                     /locus_tag="QBA20_002525"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDQ6293017.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10538..11179)
                     /gene="kdgA"
                     /locus_tag="QBA20_002526"
                     /gene_synonym="eda"
     CDS             complement(10538..11179)
                     /gene="kdgA"
                     /locus_tag="QBA20_002526"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDQ6293018.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11216..13027)
                     /gene="edd"
                     /locus_tag="QBA20_002527"
     CDS             complement(11216..13027)
                     /gene="edd"
                     /locus_tag="QBA20_002527"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDQ6293019.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13262..14737)
                     /gene="zwf"
                     /locus_tag="QBA20_002528"
     CDS             complement(13262..14737)
                     /gene="zwf"
                     /locus_tag="QBA20_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDQ6293020.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15075..15944
                     /gene="hexR"
                     /locus_tag="QBA20_002529"
                     /gene_synonym="yebK"
     CDS             15075..15944
                     /gene="hexR"
                     /locus_tag="QBA20_002529"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDQ6293021.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16072..17514
                     /gene="pyk"
                     /locus_tag="QBA20_002530"
     CDS             16072..17514
                     /gene="pyk"
                     /locus_tag="QBA20_002530"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDQ6293022.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="QBA20_002531"
                     /gene_synonym="msbB"
     CDS             complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="QBA20_002531"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDQ6293023.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="QBA20_002532"
     CDS             complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="QBA20_002532"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDQ6293024.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="QBA20_002533"
     CDS             complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="QBA20_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDQ6293025.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21085..21840
                     /gene="znuC"
                     /locus_tag="QBA20_002534"
     CDS             21085..21840
                     /gene="znuC"
                     /locus_tag="QBA20_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDQ6293026.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21837..22622
                     /gene="znuB"
                     /locus_tag="QBA20_002535"
     CDS             21837..22622
                     /gene="znuB"
                     /locus_tag="QBA20_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDQ6293027.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="QBA20_002536"
     CDS             complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="QBA20_002536"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDQ6293028.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="QBA20_002537"
     CDS             complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="QBA20_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDQ6293029.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24538..24603)
                     /gene="yobI"
                     /locus_tag="QBA20_002538"
     CDS             complement(24538..24603)
                     /gene="yobI"
                     /locus_tag="QBA20_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HDQ6293030.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24673..25275
                     /locus_tag="QBA20_002539"
     CDS             24673..25275
                     /locus_tag="QBA20_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDQ6293031.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(25277..25798)
                     /gene="ruvC"
                     /locus_tag="QBA20_002540"
     CDS             complement(25277..25798)
                     /gene="ruvC"
                     /locus_tag="QBA20_002540"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDQ6293032.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25833..26573)
                     /locus_tag="QBA20_002541"
     CDS             complement(25833..26573)
                     /locus_tag="QBA20_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDQ6293033.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26602..27054)
                     /gene="nudB"
                     /locus_tag="QBA20_002542"
     CDS             complement(26602..27054)
                     /gene="nudB"
                     /locus_tag="QBA20_002542"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDQ6293034.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPVAAALTKSWSNRQAIEQFVINAA"
     gene            complement(27172..28944)
                     /gene="aspS"
                     /locus_tag="QBA20_002543"
     CDS             complement(27172..28944)
                     /gene="aspS"
                     /locus_tag="QBA20_002543"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDQ6293035.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            29254..>29469
                     /locus_tag="QBA20_002544"
     CDS             29254..>29469
                     /locus_tag="QBA20_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HDQ6293036.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDA"
BASE COUNT         7223 a         7574 c         7240 g         7432 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta
      541 ctatggcagg ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa
      601 atacttataa aactcgggta aatcaactgg agacaataaa agaaaaatta ggaggggttt
      661 tactgacaga aataaccact cgccatatcg ccgagtttct tgatttgtag attgaaggag
      721 ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca
      781 ttgttgaagg acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag
      841 ttacaagaga acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac
      901 ttccggcatg gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag
      961 acataacgaa tatgcggttc agtgatattt atgatgaccg tctccacatc aggcaaatta
     1021 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac
     1081 ttggtgcagt agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg
     1141 ggattagaaa aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg
     1201 tcgcagccag aaaattaaca ggtatcctgt tcagtgaaaa cccaccaact tttcacgaaa
     1261 tcagaagcct ggctggacga ttgtacaaag aaatatgtgg agaagaattt gctcagcgtc
     1321 tacttggcca cacatcggag aagacaacaa aaatgtatct tgatgagaga gaaaaaacgt
     1381 acttactgct ctgattttaa cgtaaatgga ttgttaaatg tattttggtt gtgatataac
     1441 caaaaaagac cggaatacag aaattcgagt aaatttcggg gaatttcggg gagacgtttg
     1501 caactaattg attttaaata caattaaaaa aagaccgaat acgattcctg tattcggtcc
     1561 agggaaatgg ctcttgggag agagccgtgc gctaaaagtt ggcattaatg caggcttagt
     1621 tgccttgccc tttaagaata gatgacgacg ccaggttttc cagtttgcgt gcaaaatggt
     1681 caataaaaag cgcggtgctc atcagcttaa atgttaaaaa ccgcccgttc tggtgaaaga
     1741 actgaggcgg tttttttatt ggaaatcaaa aggctatttt aggtaattaa cagagttttt
     1801 cagctcgttc tataaacggt gccagactca ttttttcgcc gggattgtta ggatcatcaa
     1861 tctgaatcac cgaaatgggt tgggcattgg tcttcccact ggcaacttcc ttttgtgcga
     1921 tatcgtttaa aggatactgc acgagggtac ttgggttgat gacatacaaa gcattacccg
     1981 gtcggcaagt cagcattacc tcttcgcgat taaacgccca tttgtcttta cccacttcaa
     2041 aacgactcac ggttatcacc tgtggagccg ccagcgccgc tgcagaactg gtgagtaaca
     2101 gaaacgccag aatacttttt ttcatcattt tcgtaatcca tcaaaaaggt tcgagagtcg
     2161 caagcaggct gatgatcacc agcactaccg cacctatcgc ccattcgagt ttagtcatcc
     2221 acacaaacca gggtgccgca cgatcttcat cctgcctcat gcgcggtaca agaacataac
     2281 gattagccag cgcaatgacc accataatca tcaccaggat ggctttgagc aataagagtt
     2341 ggccccagta agtcgtaagc gtggggggaa atccggtaat caacaatgca ttaagcacgc
     2401 cgcttgccag tacgccgatc accgcaaaat gcccgcacca ggaaaaacgc atcagcgcct
     2461 gaatagccta gtgtcgccaa cggcctttga tgagttgcat acaccagagc accggcagta
     2521 atcccccaaa ccacgccgcc gcacaaatca ggtgaatcgc gtgattagtc tgatggattt
     2581 tcgctgttac cccttcattc agcgtcgcat gccccactcc cgccagcaga ataaattgcg
     2641 cggtagtgag cataaacagc aaacgtggca tattccgcgg ttgcataagg gcgacgatca
     2701 acgttacgag ggcgagaaca atttgccata accagacgcc accaaactgc gtttgcaaaa
     2761 cagcctgcca aatgtttggc gaaaatacat ctgtccatcc cgttcccatc aacccgccct
     2821 gaacggcgag cattgcagtg gcactgatca aactccagac ggcagcgtgc tgttgtaaac
     2881 gcagagaacg cttcgtcagc aagcgacgaa tcattaaggg ggccagccag gcaccataca
     2941 ttgcaaagcc aaagaccagc atcaacgagg tgaaatggat aaatcgcagc gcgatccagg
     3001 taaacgccag catgatttat ttcacgctaa aggtgtagtg tcctttcgtt ttatgcccgt
     3061 ccaccgaaac aacatgccag tcaacggtgt aggttccggg tttcagcgaa tcggccagtg
     3121 gaacaatcag ttgcttttgg tcctgctcat tccgcttcgc tggtaatgtt ttaatatttt
     3181 cgttttttgg ccccgtgatt tttgcaccac tgaatcctgt ttcaacgcct tccgagaagt
     3241 ttaaagtgat tgcctgcggg gcagctgtca cttgcgcgtt tgccgcagga tactgatgcg
     3301 ttaaatgcgc atgtgcccag acggaaggtg tcaccaacga agtggtcagg attgccagcg
     3361 cgtagcgaag ggagcgtgca gttgaagcca tattatctat tcctttttgt aataactttt
     3421 ttacagagca taaccttgtc taatgtctga gtcgaggatc atcaattccg gcttgccatc
     3481 caggctcact cttagtaact tttgcccgcg aatgatgagg agattaagaa tgctgaagaa
     3541 tctggctaaa ctggatcaaa cagaaatgga taaagtgaat gtcgatttgg cggcggccgg
     3601 ggtggctttt aaagaacgct acaatatgcc ggtgatcgct gaagcggttg aacgtgaaca
     3661 gccagaacat ttgcgcagct ggtttcgcga gcggcttatt gcccaccgtt tggcttcggt
     3721 caatctgtca cgtttacctt acgagcccaa acttaaataa aacttataca gagttacact
     3781 ttcttacata acgcctgcta aattatgagt attttctaaa ccgcccccat aatttgcagt
     3841 cattttgaaa aggaagtcat tatgtcgttc tggaaagttg cagcagcgca atatgagcct
     3901 cgtaaaacct cactcaccga acaggtagct catcatctgg agtttgtgag ggccgccgcc
     3961 aggcaacaat gtcagctgtt agtttttcca tcgctttctt tactggggtg tgattattcg
     4021 cgacgtgccc ttcctgcccc acccgatctt tcactgttag acccgctttg ttatgccgca
     4081 acgacctagc gaatgaccat cattgccggc cttcccgttg aatataacga tcgctttatt
     4141 cgtggaattg cggtgttcgc accgtggcga aaaacgcctg ggaactacca tcagagtcat
     4201 ggtgcatgtc tgggccgtcg ttccagaacc atcacggtgg tcgatgaaca accgcaaggt
     4261 atggatatgg acccgacctg ttcactgttt acgacagggc aatgtctggg agaacccgac
     4321 ctgctggcgt cggcccgtcg cttacaattt ttttcacatc agtactcgat tgccgtgctg
     4381 atggcaaatg cccgtggtaa cagtgcacta tgggatgaat atggtcgtct catcgttcgc
     4441 gccgatcgtg gttcattatt gttagtcggt cagcgttcat cacagggttg gcaaggcgat
     4501 atcattccat tacgctaggc tttttcggcc tggagcatgc catgttgcgc attatcgata
     4561 cagaaacctg cggtttgcag ggagggatcg ttgagattgc ctctgttgat gtcattgacg
     4621 gaaaaatcgt caaccccatg agcctcctgg tgcgccccga tcgtcctatt agtccacaag
     4681 cgatggcgat tcatcgcatc accgaagcca tggtcgccga taaaccgtgg attgaagatg
     4741 tgatcccaca ctattacggt agtgaatggt atgtcgcgca caacgccagc tttgaccgcc
     4801 gcgtactgcc tgagatgccc ggtgagtgga tttgcactat gaaactggcc cgtcgtttgt
     4861 ggccagggct caagtacagc aatatggcgt tatataaaac acgcaagctc aatgtacaga
     4921 caccgccggg cctgcatcat caccgcgcgt tgtatgactg ttatatcacc gccgcgttgc
     4981 ttatcgatat tatgaacacc tccggctgga cggcagaaca gatggccgat atcaccggac
     5041 gtccgtcgtt gatgacgacc ttcacctttg gcaaataccg tggcaaagcg gtttccgacg
     5101 ttgccgaacg ctatccgggc tatctgcgct ggttatttaa taacctggac agcatgagcc
     5161 cggagctgcg tttaacactg aaacattatc tggaaaatac ttaatccgca ggcgtagcgg
     5221 gtaatgttcc ctgcgccagc gcgaccagaa aagcatattc catcgctacg ccttcgtacg
     5281 atttaaagcg gccagattta ccgccatggc ctgagtccat gtcggtacag agcaataaaa
     5341 gatggtcatc ggttttcagc tcgcgcaatt tagcgaccca ttttgccggt tcccaatatt
     5401 gcacctgaga atcgtgtaaa ccggtcgtta ccagtaaatg cggataagcc tgtgcggtga
     5461 cgttgtcata cgggctgtag cttttcatgt actcgtaata ttgcggatcc tgcggatttc
     5521 cccactcttc aaactcacca gtggtaagag gaattgattc atcaagcatc gttgttacaa
     5581 catcaacaaa cggtacctgg gcgataacgc cgtggaataa ttccgggcgt tgattaattg
     5641 caacgcccat caacatgccc cccgcacttc cgcccatcgc ataacagagc gaaggagagc
     5701 catagcccag ttttaacaat gcatcgcagg catcaagata atcattaaac gtatttttct
     5761 tcttcagaaa tttaccgtct tcgtaccatt gttgccccag ctcaccaccg ccgcgaacat
     5821 ggacaatggc gtagacaaag ccacgatcta acaaactcaa gcggctaaaa ctgaaatcgg
     5881 catcaatact tgcgccgtaa gaaccatagc catacaccag caacgggttg tgtcctttgc
     5941 gaaaatgttt gcgatgatag accagcgaaa ccggaacttc gacgccatca cgggcgacta
     6001 tccacaggtg ttcactgcgg taattcgccg catcaaaacc aggcacttct gtttgtttta
     6061 atacacgacg ctcaccggta tccatatcca gttcaaacaa agtgtctggt gtggtcatgg
     6121 aagaataacc ataacgcaat cgcgcggttt caggttctgg attgtaggca atccaggtca
     6181 cataggccgg atcatcaaag gcaatgccaa tgacttcccg ggtcttgcgg ttaatttggc
     6241 gcaaactggt taacccgcgc tgacgctctt caaccaccag ccagtcggta aacagcgtaa
     6301 acccttccag catgatgttt tcgcgtggcg gaattaactc ttcccactgt tgctcatcac
     6361 gcatacgggt acggtataag ccaaagtttt tgccgtggcg attggaacgc agataaaaac
     6421 gatgctggta gtgatcaagg ctgtattcgt gatctttgcg gcgcggcaga aaaacaaacg
     6481 gctcggcatc ggccatttcc gcgtccagca ggcgaacttc actggtggtg gcgctggcca
     6541 gatgaatgac gacatagtgc ttcgacgtcg ttttatgcag gctgacataa taggtatcgt
     6601 ctttttcttc gtagatcagt gtatcttgcg atgctggcgt accgatggcg tgacgccaga
     6661 cctgataagg cagcagcgtc accggatgct tgcgaacata gtagaaagtc caggagtcat
     6721 ttgcccagac aaagctgggt tcaacgttat ccagcagttc cgggtaccag ttacctgttt
     6781 ccagattacg aaaacgaatg ccgtactggc gtcgggaaag aaaatcttct gccagcgcca
     6841 taatggtgtt atcgggcgta atcgccattc cgcccatcga ataaaactca ctatgagctg
     6901 cgcgcttgtt ggcatcgagc aatgtttccc actcatccca ctcttcgctg aatgccgatt
     6961 gacgctggta gatagcatat tcacagcctg gttcataaat atgccgatag cggtagccat
     7021 tttttatgta gggcgcagaa acttctcttt gcggaatgcg gtcgatgatt tcctttaaga
     7081 tgcgatcctg caaggcttgt tgtgaggcca tcacccgatg accgtaacta ttttcttgtt
     7141 gcaggtagtc caggactttt ggctgagaac gcgtatcgtc ccgcagccag tagtaattat
     7201 cgatgcgcgt atcgccatga agcgtcatgg cgtggggaat gcgggcggct tttggtagca
     7261 tgttattgtt ctttctggtt ggaaacatct tataagggtg gcaaaactca ccggggatgc
     7321 aagcgaaaca gggtcgtcat tgcttagatg atgacaggta atggcgcgga tatcgaatgt
     7381 tatgcagaca gagaaaatca gcctgttcaa atggctgtgc ggttctggat agcccgaaat
     7441 agtcaacttc aggctatcca gagagcggaa ttattccgcc aaagtgcgtt tttgctgttc
     7501 gagatcgcgt tcaatgcctt cacgaacatc ctgggggatt ttcagcgcgt cacccagtgc
     7561 attcaggtaa ctgcgttcca taaaatggtc aatatcaata gccgcgcagc tcaggaaata
     7621 gatttccagc gcctcttctt cattgcggac cccggtagcc aggcgttgtg gatccagcgg
     7681 ttgttcgatt gcctgctcaa tgagtacacg cccctgctct tccacgccgg cttcacgcaa
     7741 ttgctggtcg attgccgcac gttcattggc atcaatatga ccatcacttt tagcggcaaa
     7801 gaccagcgca aggatcaaac gttccgtacg ctcatccagc ggcgtacttt gcgcgccaaa
     7861 ctgcggttcg tcctgatgcg ccgcgcgaat tttatctttg tatttattcc acagcaccgt
     7921 acccgctacc gctccgccgc caaccagtaa cgcgttggtg ccatatttcg taagaagttt
     7981 acgtgctgat ttatttgcga ccagcagccc agccagtccg cctaatgcgc ctgacactaa
     8041 cagtttgccc aatccctgat ccgcagacga ggaggtagaa gaactgcttt gcccaagcag
     8101 ggattgcagt tgatttaacc agttagccat atttgctcct caataaccat ttttagtgcc
     8161 tgtagcatag cggatgagga tgtcagaaaa tgtatatcga gacaaaagat gcgcaaataa
     8221 gcggggcggt aggccgttat tcaaagaaat tcgcgccagg taaggtattt actgtatatc
     8281 aagtgataag ggtattttag gtgaaagtag tttgtcgttt tgagtggcat tgtttgatgc
     8341 tgattgcccg atgcgacgct gttgcgtctt ctcaggccta cgattgtggt gcgttctggg
     8401 acggacaagg cactcgcgcc gcatccgcca acggtcaatc gaattatcag aagccattaa
     8461 cgccgctgat attccgccat tcccgctttg cagtccacgc tgacctggta atgaatatcg
     8521 gcacttttac cacgcacggt tagcggtact gaccatttat catctttacc ctgaatgtcc
     8581 tgcaaactga cccaggccac gggatcggcc tgaccgacaa ttttttgatc atctgcccag
     8641 cgcgccacgc gattttgttg ataatcacgt tttacgctcg cggcaattcc ggcagcatcc
     8701 agaccttcac actttgggaa agtgaccgac ttgctggttt cattattggc agcgaaaact
     8761 gatgcgcagg cagaaaccaa caaccgccct aaaaacgccc ctcttttttt catgtttttc
     8821 tccatagcac aatgattcag gagaaagcat ggtacaaatt gtcaggagcg caagttgctt
     8881 caggccgcgt gtgaggcatc ttcctcttcg gattcagcac cgacgacctg tggggacggt
     8941 aatttgccgg ttttcagaat ggtgctcagc acctcttttt gttctgccag ccatagcgaa
     9001 agggcttcac gttgctcgtc ttccatttgc actggagaat ttgtcagcta ggtgtcaagc
     9061 agatccgccg tatcaagcat tttgtcataa gcatcggctt cctttttgct ggtaaacgac
     9121 attttctctt cgccctcacg aatgactacg tatttaactt caaccgccat ttgcagcctc
     9181 tcataataac tgagatttta tacagtatat ttcttttcgg ttgagaaatc aacatcagca
     9241 ataaagacac acgcaaacgt tttcgtttat actgcgcgcg gaattaatca ggggatattc
     9301 gttatgacgt tattaggcac tgcgctgcgt ccggcagcaa ctcgcgtgat gttattaggc
     9361 tccggtgaac tgggtaaaga agtggcaatc gagtgtcagc gtctcggcgt agaggtgatt
     9421 gccgtcgatc gctatgccga cgcaccagcc atgcatgtcg cgcatcgctc ccatgtcatt
     9481 aatatgcttg atggtgatgc attacgccgt gtggttgaac tggaaaaacc acattatatc
     9541 gtgccggaga tcgaagctat tgccaccgat atgctgatcc aacttgaaga ggaaggactg
     9601 aatgttgtcc cctgcgctcg cgcaacgaaa ttaacgatga atcgcgaggg tatccgtcgc
     9661 ctggcggcag aagagctgca gctgcccact tccacttatc gttttgccga tagcaaaagc
     9721 cttttccgcg aggcggttgc cgccattggc tatccctgca ttgtaaaacc ggtgatgagc
     9781 tcttccggca aggggcagac gtttattcgc tctgcagagc aacttgctca ggcatgggaa
     9841 tacgctcagc aaggcggtcg cgccggagcg ggccgcgtaa ttgttgaagg cgtcgttaag
     9901 tttgacttcg aaattaccct gctaaccgtc agcgcggtgg atggcgtcca tttctgtgca
     9961 ccagtaggtc atcgccagga agatggcgac taccgtgaat cctggcaacc acagcaaatg
    10021 agcccgcttg cccttgaacg tgcgcaggag attgcccgta aagtggtgct ggcactgggc
    10081 ggttatggtc tgtttggtgt cgagctgttt gtctgtggtg atgaggtgat tttcagtgag
    10141 gtctcccctc gtccacatga taccgggatg gtgacgttaa tttctcaaga tctctcagag
    10201 tttgccctgc atgtacgtgc cttcctcgga cttcctgttg gcgggatccg tcagtatggt
    10261 cctgcagctt ctgccgttat tctgccacaa ctgaccagtc agaatgtcac gtttgataat
    10321 gtgcagaatg ccgtaggcgc agatttgcag attcgtttat ttggtaagcc ggaaattgat
    10381 ggcagccgtc gtctgggggt ggtactggct acttcagaga gtgttgttga cgccattgaa
    10441 cgcgcgaagc acgccgccgg acaggtaaaa gtacagggtt aaacccggca aaaaaacgct
    10501 acaaaaatgc ccgatcctag atcgggcatt ttgactttta cagcttagcg ccttctacag
    10561 cttcacgcgc cagcttagta atgcggtcgt aatcgcccgc ttccagcgca tctgccggaa
    10621 ccagccagga accaccgatg cacagcacgc ttttcagtgc caggtagtca cggtagttag
    10681 ccggagaaat accacccgtc gggcagaaac ggacctggga gaacggaccc gcgatcgcct
    10741 gcagggcttt cacgccgccg ttagcttcag ccgggaagaa tttgaactct ttcaaaccgt
    10801 agtccatacc cagcatcagt tcggaaacag tgctgatccc cggaatcaga ggaatagtcc
    10861 cttcggtagc agctttcagc agcggctcgg tcagacccgg gctaattgcg aactgtgcac
    10921 ccgcttcagt gacttctgcc agctgctgtg gattcagcac cgtaccggca cccacaatcg
    10981 cttcaggcac ttctttggcg atagcacgga tagcgtcaac tgcacactcg gtacgcagag
    11041 tcacttccag aacgcgcacc ccaccagcaa ccaacgcttt tgccatcggc accgcgtgtt
    11101 ccagtttttt taccacgata accggtacaa ccgggccggt ggtcaggatt gattctgcac
    11161 ttgttttcca gtttttcatc agagttttct ctcgcctgat tacaaatttg tcgtcttaaa
    11221 aagtgataca ggttgcgccc tgttcggcac cggacagttt ttcacgcaag gcgctgaata
    11281 attcacgtcc tgttcccacg cgtgacgcgc tcaggtcagg aatgtgcggt tcgcgagcag
    11341 ccagttccgc ttcgtctacc agcagtgtca gttcgcctgt ctgtccattc acacgaatga
    11401 tgtccccgtc gcgcactttt gccagcagcc cgccatcgta ggcttctggt gttacgtgga
    11461 tagctgacgg cactttacct gaagcgccgg agagtcgtcc atcggtaact aacgcaattt
    11521 tgaaacaccg gtccaataat acaccaagtg gcggcatgag tttatgtaat tctggcattc
    11581 cgttcgcttt tggcccctga tgacggacaa caacgacaca atcgcggtcc agcaaacccg
    11641 cttcaaaggc cggcataacg tcatgctggc tttcaaaaac aaccgctggc gcttcaatca
    11701 cctggttctc aaccggcacg gcagaggttt tcataaccgc acggcccagg ttaccgctta
    11761 acacttttgt cccaccatga tgagagaaag gttgttcgaa ggaagcgatc acattgctgt
    11821 cgagtgattt ttccgccccc tcccgccagt ccagttcacc attattcagc catggttcaa
    11881 gggtataacg agacagacca aagcctgcca ccgtattgac atcttcatgc agcaggcctg
    11941 ctttgagcag ttcacgcacc agaaccggta cgccacctgc cgcctggaag tggttaatat
    12001 cggccggacc gttcgggtag agacgtgcca tcagcggtac aacatcagaa aggtcagaga
    12061 agtcatccca gttaatctga ataccggccg cgcgcgccat cgccaccagg tgcatggtgt
    12121 ggttagtgga accaccggtc gccagcagtg caacgatacc gttcaccacc actttctcat
    12181 cgatcatctt accgatcggc atccattcat taccattacc ggtcatgcgt gtaacctgac
    12241 gcgcagcggc agcggtcaaa gcatcgcgca gcggagaatc cggatgaaca aaagaagagc
    12301 ctggcaactg catccccata aattccacca ccatctggtt ggtgttggca gtaccgtaga
    12361 aagtacatgt tcccggcgca tggtaagacg cggcttctga ctccagtaag gccatgcggt
    12421 ccactttacc ttcggcataa agctggcgaa tacgcacttt ttctttattt ggcaaaccgc
    12481 ttgccatcgg tccagacggc acaaacaccg caggcaaatg accaaacgac agggctgcca
    12541 tcgtcagacc cgggacaatc ttgtcgcaca caccgaggaa cagagcacca tcaaacatgt
    12601 tatgggacag ccccaccgcc gcagacatcg ctatcacttc gcggcttagc agcgacaatt
    12661 ccattccatc ctgcccctgg gtgacaccat cacacatcgc cggaacaccg cccgcaacct
    12721 gaccaaccgc attcgcttca tgcagggctt tacgaatgat ttctggatag tgttcataag
    12781 gctggtgcgc ggagagcatg tcgttatagg aggtgatgat ggcgatattg ttacgcaaca
    12841 tgcttttcaa agaggctttg tcttctggct ggcaggcagc gaaaccgtgt gccaggttac
    12901 cgcatgccaa ctgcgaacga tgaacggtcg acgttttcgc ttgttctatc cgggcgagat
    12961 aagcagagcg agtctcgcgc gaacgttcaa tgattcgatt tgttacgcgt aacaattgtg
    13021 gattcataaa ggctcctgaa attgagttgt cagagcagga tgattcacaa cgcgtttcat
    13081 tcagaggatt tatgagtgaa acgcctgtaa ccggagctca tagggcaaaa acgtttcagt
    13141 cagtgtaata aaaaaagcct cgtgggtgaa tccgcacgag gcctgaaagt gtaaaaattg
    13201 ttctacaatc tgtgcaagat catgttaccg gtaaaataac cataaaggat aagcgctgat
    13261 attactcaaa ctcattccag gaacgaccat cacgggtaat catcgccacc gaggcaacgg
    13321 gtccccaggt tccggcctga tacggtttcg gcgcatcatt gtccatcgcc cacgcctcag
    13381 taatggagtc tacccatttc caggcttctt ccacttcgtc gcgacgtaca aacagtgcct
    13441 gaataccacg catggtttcc agcagcaaac gttcataggc atccgccaga tgcgtctgat
    13501 taaaggtttc tgaatagctc agatccagct tggtgatttg caggttatgt ttgtggtcaa
    13561 ggccaggaac tttattcagt acctggatat ccacgccttc atcaggttgc agacggatag
    13621 tcagtttatt ctgcggcaga tcctgccacg actctttaaa cagattcagt tcaggtgttt
    13681 tgaaatagac cacgacttca gaacatttgg tcggcagacg tttaccagta cgcaggtaga
    13741 atggcacacc ggcccagcgc cagttatcaa tgtcgacgcg gatcgccacg aaggtttctg
    13801 tattgctgct tttgttcgcg ccctcttctt ccagatatcc cggcactttt ttgccctggg
    13861 cgaagcccgc agtatattgc ccgcgtacgg ttttttcgcg tacgttggag cggtcgatgc
    13921 ggcgcaggga cttcagtact ttcacttttt catcgcggat gctgtctgcg ctcaggtcag
    13981 atggcggaga catcgcaatc atgcagagaa tttgtagcag gtggttctga atcatgtcac
    14041 gcatctgacc ggctttatca aaatagcccc agcgcccttc gatccccact tcttctgcca
    14101 cggtaatctc aacatgatca atggtgcgat tgtcccagtt attcacaaac agggagttag
    14161 caaaacgcag cgccaacagg ttcagcaccg tttctttacc aagatagtgg tcgatacggt
    14221 aaacctggca ctcctcgaag tattcgccaa cctgatcatt gatttcctgc gaggtcgcca
    14281 gcgacgtccc cagcggtttc tccatgacta cgcgtgccgg tttagcattc agttttgcct
    14341 cgccaagccc tttgcaaatt gcgccaaaag tgctgggcgg catggcaaag tagttaatag
    14401 tgatacgatt tttttgatcc agcatcgcgc cgagacggtt gaatgcagca gtgtcattga
    14461 catcgagatt acaaaaatcc agacgtgcac tcagggtgtc ccataaacct tcatcaatgg
    14521 tttctttcat gaaagtttcg agcgcctcgc ggacaacttt ggtatatgcc gctttatccc
    14581 agtcagcacg ccctacgccg ataatccggg tgtccgggtt gagctgaccg actttttcca
    14641 gttgatacag ggaaggcagc aatttacgac gcgcaaggtc gcctttcgcg ccgaaaatga
    14701 ccaggtcaca ggcctgggct gtttgcgtta ccgccatgtc attctcctta agttaactaa
    14761 cccggtactt aagccagggt atacttgtaa ttttcttacg gtgcactgta ctgcttttac
    14821 gagcttgcga aaactgtaaa cgcttatcca cccgtgcgat tacgggaaaa gcgcgcaaag
    14881 tgcggcaaaa ctgataaaaa aatcatcgtt ttccattcgg taaaaatctg acactgatca
    14941 tgttatgaaa aaaaataaca acttttttat ctgcttttgt cattaacacg gcacacaggc
    15001 gtaatatctg acaaaactgc atttcgattt ctttcagtgc ggaaatcgtc attacccgtg
    15061 agtctcttta catcatgaat atgctggaaa aaatccagtc tcagctggaa catttgagca
    15121 aatcagagcg caaagttgcc gaggtcattc tggcttcgcc cgataacgcg atccattcga
    15181 gtattgctgc tctggcgctg gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc
    15241 gcagcatgga cacgcgcggt tttcctgatt ttaaacttca tctggcacaa agtctggcga
    15301 atggcactcc ctatgttaat cgcaacgtca atgaagatga cagcgttgaa tcatacacgg
    15361 ggaaaatatt tgagtccgca atggcaacgc tcgatcatgt ccgtcattca ctggataaat
    15421 ctgccatcaa ccgcgccgtc gacttgctca ctcaggcaaa aaaaatcgcc tttttcggat
    15481 taggctcttc tgccgccgtt gcccacgatg cgatgaataa gttctttcgt tttaatgttc
    15541 cggtggtgta ctccgatgat atcgtgctgc aacgcatgag ttgtatgaat tgtagcgacg
    15601 gagacgtggt ggtgctgatt tctcacacag gaagaacaaa aaatctggtc gagctggcgc
    15661 agctggcacg cgaaaacgac gccatggtga ttgccctcac ctctgcgggt accccgctcg
    15721 cccgggaagc aacgctggca attaccctcg acgtaccgga agatactgac atttatatgc
    15781 ccatggtttc tcggcttgca cagctgaccg tgatagatgt gctggcgaca ggatttactt
    15841 tgcgacgcgg tgcaaaattc agagataact tgaagcgggt caaggaagcg ctgaaggaat
    15901 cgcgttttga taagcagtta cttaatttaa gtgacgatcg ctaaaagcga ctgtcactgt
    15961 cctaatctta tacgacatcc gaatgagatt aatttatcgc catcgcggcg ttatttcatt
    16021 cggatttcat gttcaagcaa cacctggttg tttcagtcaa cggagtatta catgtccaaa
    16081 aggcttcgca gaacaaaaat cgttaccacg ttaggcccag caacagatcg cgataataat
    16141 cttgaaaaag ttatcgcggc gggtgccaac gttgtacgta tgaacttttc tcacggctcg
    16201 cctgaagatc acaaaatgcg cgcggataaa gttcgtgaga ttgccgcaaa actggggcgt
    16261 catgtggcta ttctgggtga cctccagggg cccaaaatcc gtgtatccac ctttaaagaa
    16321 ggcaaagttt tcctcaatat tggggataaa ttcctgctcg acgccaacct gggtaaaggt
    16381 gaaggcgaca aagaaaaagt cggtatcgac tacaaaggcc tgcctgctga cgtcgtgcct
    16441 ggtgacatcc tgctgctgga cgatggtcgc gtccagttaa aagtactgga agttcagggc
    16501 atgaaagtgt tcaccgaagt caccgtcggt ggtcccctct ccaacaataa aggtatcaac
    16561 aaacttggcg gcggtttgtc ggctgaagcg ctgaccgaaa aagacaaagc agacattaag
    16621 actgcggcgt tgattggcgt agattacctg gctgtctcct tcccacgctg tggcgaagat
    16681 ctgaactatg cccgtcgcct ggcacgcgat gcaggatgtg atgcgaaaat tgttgccaag
    16741 gttgaacgtg cggaagccgt ttgcagccag gatgcaatgg atgacatcat cctcgcctct
    16801 gacgtggtaa tggttgcacg tggcgacctc ggtgtggaaa ttggcgaccc ggaactggtc
    16861 ggcattcaga aagcgttgat ccgtcgtgcg cgtcagctaa accgagcggt aatcacggcg
    16921 acccagatga tggagtcaat gattactaac ccgatgccga cgcgtgcaga agtcatggac
    16981 gtagcaaacg ccgttctgga tggtactgac gctgtgatgc tgtctgcaga aactgccgct
    17041 gggcagtatc cgtcagaaac tgttgcagcc atggcgcgcg tttgcctggg tgcggaaaaa
    17101 atcccgagca tcaacgtttc taaacaccgt ctggacgttc agttcgacaa tgtggaagaa
    17161 gctattgcca tgtcggcaat gtacgcagct aaccacctga agggcgttac ggcgatcatt
    17221 accatgaccg aatcgggtcg taccgcgctg atgacctccc gtatcagctc tggtctacca
    17281 attttcgcca tgtcgcgcca tgaacgtacg ctgaacctga ctgctctcta tcgtggcgtt
    17341 acgccggtgc actttgatag cgctaatgac ggcgtagcag ctgccagcga agcggttaat
    17401 ctgctgcgcg ataaaggtta cttgatgtct ggtgacctgg tgattgtcac ccaaggcgac
    17461 gtgatgagta ccgtgggttc tactaatacc acgcgtattt taacggtaga gtaagtacgt
    17521 tgccggatgc ggcgaaaacg ccacatccgg cctatagttc aatgatagtt caacagattt
    17581 cgaatattct gaagcaaact tgaacttatc atcaggcgaa ggcctctcct cgcgagaggc
    17641 ttttttattt gatgggataa agatctttgc gcttatacgg ctggatttcg cccggtttgc
    17701 gagttttcag caattttagt atccaggtgt attgttctgg tcgcggacca acaaaaatct
    17761 cgacttcttc attcatccgc cgctcaatcg tatgatcatc cgcctctaac agatcatcca
    17821 tcggtgggcg cacctgaatc gtcagacgat gcgtcttgcc atcataaatc ggaaacagcg
    17881 gtacaacgcg cgcacggcat actttcatca aacgaccaat cgcgggcaac gtcgctttat
    17941 aggtggcaaa gaaatccaca aattcgctgt gctctgggcc atgatcctga tcgggtaaat
    18001 aatatcccca gtacccctga cgtaccgact ggatgaatgg tttaataccg tcatttctcg
    18061 catgcagacg accgccaaag cgacgacgca ccgtgttcca gacataatca aaaaccgggt
    18121 tgccctgatt atggaacatc gctgccattt tctgcccttg cgaggccatc agcatggcag
    18181 gaatatcgac ggcccaaccg tgcggcacca gaaagataac tttctcgtta ttacgccgca
    18241 tctcttcgat gatctccagc ccttgccagc caacgcgcgg ctgaattttc tccggcccgc
    18301 gtattgccaa ctcagccatc attgccatcg cttgcggcgc ggtggcaaac atctcatcaa
    18361 caatcgcttc gcgttcagct tcactacgtt ctggaaagca gagcgacaga ttgattaacg
    18421 cacgacggcg tgagcttttt cccagtcgtc cggcaaaacg tcccagccgt gccagaatgg
    18481 gatcacggaa ctttggcggc gttaaagcga tacccgccat cgctgctaca cccagccatg
    18541 ctccccagta gcgcgggtgg cgaaaggatt tatcaaactc aggaatgtat tcgctattat
    18601 tttttttcgt ttccatgctt ttccagtttc ggataaggca aaaatcaatc tggtgatagt
    18661 gtagcggcac aacttgcccc gcaccaaata aaaaaagccg gtactgactg cgtaccggct
    18721 gcgaatggat gttaattaat caaaccgtag ctgcggcaca atctctttgg cctgcgccag
    18781 gaattcgcga cgatcggagc cggtcagccc ttcggtacgc ggcagttttg ccgtcagcgg
    18841 gtttacggcc tgctggttta tccatacttc atagtgcaga tgcggcccag ttgaacgtcc
    18901 ggtattaccg gaaagcgcga tacggtcgcc acgtttcacc ttctgtcccg gtttcaccag
    18961 aatcttgcgc aagtgcatat aacgcgtggt gtagctgcga ccatgacgaa tagccacata
    19021 ataacctgct gcgccactgc gtttggcaac caccacttca ccgtcaccca ctgaaagcac
    19081 tggcgtacct tgtggcatgg cgaaatcaac acctctgtgt ggtgcaacgc gacctgtcac
    19141 cggattagtt cgacgcgggt taaagttaga cgagatacgg aactgtttcg ccgtcgggaa
    19201 tcgcaagaat cctttcgcca gaccagtacc gttacgatcg tagaatttgc catcctcagc
    19261 gcggattgcg taataatctt taccttctga acgcaaacgt acgcccagca gctggctttg
    19321 ctcacgttta ccatcaagca tttctcgaga cattaatacc gcaaattcat cgcctttttt
    19381 cagtttgcgg aaatccattt gccactgcat ggctttaatc actgcgctca cttcggcgct
    19441 ggttaaaccg gcgtttctgg cgctggcaac aaagctccct ccgacggtac ctttcagcag
    19501 attgttaacc cactctcctt gctgcatttc gctggtcatt ttaaaaccgt tagcggcagt
    19561 acggtcatag gttcgggttt cacgacgaga cacttcccag gtgaggcgct gcagttcgcc
    19621 gtccgcggtt aatgtccagg agagttgttg accgattttc aggttacgca attctttgtc
    19681 ggcagcagca agttgggtga tatcacccat atcaatacca tactgattga gaatgctgct
    19741 tagcgtatcg ccagtggaaa caacatattc atgcacgccc gcttcaccgg cgattttgtc
    19801 atccagttcg tcctggggaa tggcttcatc ttcttgtgca gcttgatcaa tcggctcact
    19861 ggcttcaggt aagagcgaac gaatttcgtt ctgttccagc tcaatggttt tgacaattgg
    19921 cgtggcatca cggtgataaa catagggccg ccagacagcg acggccagag taagaacggt
    19981 gagcgacccc aacataacgc ggtgtggtcg cggtaaatta ttaaacgcca gggcgacaga
    20041 gcgggctatc tgttgcacgt aatcacttcc tcattaatct cctttcaggc agctcgcata
    20101 ctggttggct aattgactca ggaattctga atagcttgtt ttacccagtt tgatattcgt
    20161 ccccagggga tccaacgttc ccatacgaac ggatgtccct cgtgcgacgc tctcaacgac
    20221 cgctggcctg aactgtggct cagcaaaaac gcaggttgct ttttgctcaa ccaactgtgt
    20281 tcttatttca tgtaaacgct gcgcgccagg ttgaatctca gggttaacgg taaaatgacc
    20341 aagcggtgtc agtccgaact gtttttcgaa atagccgtaa gcatcgtgaa aaacgaaata
    20401 acctttcccc ttgagcggcg cgagctcgtt accaacctgc gtttcggttg aggccaattg
    20461 tgcctcaaaa tccttcaggt tggcgtcaag tttggctcga ctttgcggca taagttccac
    20521 taattttcca tggattgcaa ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg
    20581 catgttgaaa tcgccgtgat ggtgatcttc gtcacttttt tccgcgtggt cgtgatcatc
    20641 atcatcgccg tgaatacttt tcatcagtag cggtttcaca tcttcaagct gcgcaatcgt
    20701 tacctgcttc gcttctggta atttgcttac cggtttttgc ataaacgctt ccatctccgg
    20761 gccaacccaa acgactaagt ccgcgttctg taagcgtttt acatccgatg gacgcagtga
    20821 ataatcatgt tctgaagcgc cgtcaggcag taaaacctcc gtttctgtta caccatcagc
    20881 aatggcagaa gcgatgaacc caacgggttt aagcgaagcg acaacggcgg catctgcggc
    20941 ctgtgttgca cctccccaga gagcggcgga taatgctgcg aaaagaagcg tttttttatg
    21001 taacataatg cgaccaatca tcgtaatgaa tatgagaagt gtgatattat aacatttcat
    21061 gactactgca agactaaaat taacatgaca agtctggttt ccctggaaaa tgtctcggtt
    21121 tcttttggcc aacgccgcgt cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt
    21181 ttgactttac ttgggccaaa tggcgcaggt aagtcgacac tggtacgggt agtgctcggg
    21241 ctggtaacac ccgatgaagg ggttatcaag cgcaacggaa aactgcgcat cggctatgta
    21301 ccgcagaagc tgtatctcga caccacgttg ccactgaccg taaaccgttt tttacgctta
    21361 cgccctggta cacataaaga agatattttg cctgcactga aacgtgtcca ggccgggcac
    21421 ctgattaacg caccgatgca aaagctctct ggtggcgaaa cgcagcgtgt actattagcg
    21481 cgagcattgt taaattgccc gcaattatta gtgctggatg aacccactca aggcgtggat
    21541 gtaaatggcc aagtggcgtt atatgacctt attgaccagt tgcgtcgtga actggattgt
    21601 ggcgttttaa tggtttctca cgatctgcat ctggtgatgg caaaaaccga tgaagtgctg
    21661 tgcctgaatc accacatttg ttgttccggc acaccggaag ttgtttccct gcatccggag
    21721 tttatttcaa tgtttggtcc tcgtggtgct gaacaactgg gtatctatcg ccatcatcat
    21781 aatcatcgtc acgatttaca gggacgaatt gttttgcgtc ggggaaatga tcgctcatga
    21841 ttgaattatt atttcccggt tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc
    21901 tgggttcgtt tgtagtctgg cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct
    21961 cattacttgg tgtcgcgttt ggtttgttgc tggacgtgaa tccattctat gcggtgattg
    22021 ccgttacgct gctgctggcg ggcggtctgg tatggctgga gaagcgtcca cagctggcga
    22081 tcgacacgtt attagggatt atggcgcaca gtgccctgtc gctgggccta gtggtcgtta
    22141 gtctgatgtc taacattcgt gttgatttga tggcttacct gttcggtgat ttactggcag
    22201 tgacgccaga agatctcatc tctattgcga ttggcgtggt catcgtggtg gctattttgt
    22261 tctggcaatg gcgcaatttg ctgtcgatga cgattagccc ggatctggcg tttgttgatg
    22321 gtgtgaaatt acagcaagtg aaattgttgt tgatgctggt gacggcattg acgattggtg
    22381 tagcgatgaa atttgtcggt gcgttgatta ttacttcgtt gctgattatt cctgcagcta
    22441 ctgcgcgtcg ctttgcccgt acgccggaac agatggctgg tgtcgctgtt ttggtgggga
    22501 tggtggcagt aactggcggt ttaacctttt ccgcgtttta cgatacgccg gcgggtccgt
    22561 cggtggtgct atgtgcggca ctgttattta ttatcagtat gatgaaaaag caggccagct
    22621 aatctatcgc tgaacacatt tgtcggatgc ggcgcgagcg ccttatccga cctacggttc
    22681 gctatctctg gctggcctga taagacgcga acagtgtcgc atcagacaca ctgccagtgt
    22741 cggatgcggc gcgagcgacc aatccgactt acggcatttc tggcggcgtt atgccaaagt
    22801 gattccacgc ccgcgtcgtc gccatacgcc cgcgcggtgt acgctgcaaa aagccttgct
    22861 gaatcaaata aggttccagt acatcttcaa tggtttcacg ttcttcgcca atggctgccg
    22921 ccaggttatc cagacctacc ggcccaccaa agaacttatc gattaccgcc agcaacaatt
    22981 tgcggtccat ataatcgaaa ccttcagcat cgacattcaa catatccagc gcctgagcag
    23041 cgatatctgc cgagatggtg ccatcgtgct tcacttcggc gaaatcacgc actcgacgca
    23101 gcagacggtt ggcaatacgt ggcgtaccgc gagcgcgacg agctacttcc tgcgcaccgt
    23161 catcactcat ctcaagcccc ataaagcgtg cactgcgact gacgatatat tgcagatccg
    23221 gcacctgata aaactccaga cgttgcacaa taccaaaacg gtcgcgcaac ggtgatgtca
    23281 gcgaacctgc gcgcgtggtt gcgccaatca gggtaaacgg cggcaaatca attttaatgg
    23341 agcgtgccgc cggaccttca ccaatcatga tatccagttg gtagtcttcc attgccgggt
    23401 acagcacttc ttcaacaact ggcgataaac ggtggatctc atcaataaac agcacgtcat
    23461 gcggttcaag gttagtgagc atcgcagcca aatcgcccgc cttttccagc accggaccag
    23521 aagtcgtgcg taaattaacg cccatttcat tggcgacaat gttggcaagc gtagttttac
    23581 ccaaccccgg aggaccaaaa atcaacaaat gatcgagggc atcgccgcgc agtttcgctg
    23641 ctttgatgaa aatctccatc tgtgaacgaa cctgcggctg accaacatac tcttccagta
    23701 atttagggcg aatggcgcga tctgctacat cttccggcaa agtggtaccg gcagaaatca
    23761 gacggtctgc ttcaatcatc ctttacctca taacgcggcg cgtagggctt cgcgaattaa
    23821 tgtttcactg ctggtgtcag ggcgagcgat tttgctcacc atgcggcttg cttcttgtgg
    23881 tttatagcct agcgccacca gcgcggcaac tgcttcttgt tcagcatcgt cggtcgccgg
    23941 gctagcagga gacgtgagta ccaggtcggc ggctggcgta aagagatcgc catgcaaacc
    24001 tttaaatcgg tctttcattt caacaatcaa gcgttcggcg gtttttttgc caatacccgg
    24061 cagtttcacc agtgccccca cttcttcacg ctcaacggca ttaacgaact gctgcgctga
    24121 cattccggag aggatcgcca gggccaactt cgggccgacg ccgttggttt tgatcaactc
    24181 tttgaacaac gtgcgttctt gtttattgtt aaaaccgtac agcagttgcg cgtcttcacg
    24241 caccacaaag tgggtgaaaa cgatcgcttc ctgacccgct tcagggagtt cataaaaaca
    24301 ggtcatcggc atatgcactt catagcctac gccgcccact tcaattaaca ccagcggggg
    24361 ttgtttttca atgatgatgc ctctgagtct gcctatcaca tgacgctcct gcgtaatgaa
    24421 tcaaagataa tgctgtatga taaaaaaatg ctggatagat atccagcgaa ggatgaagaa
    24481 aacttgcgag gtgtcccgat gatctgaaaa atggcacagt ataatttatt caacaggtta
    24541 tattggaagc aaatatttga atattacata ttcagtgaag aaatgcgtaa taaaaatata
    24601 cattgcgact cctgaaaaat aattttttta tgctattacg tatattcgta tctatttcaa
    24661 tggaatgaca acgtgaatat taattaccct gctgaatatg aaattggaga tatcgtcttt
    24721 acatgtatag gtgctgcctt atttggtcaa atatcagctg catcaaattg ctggagtaat
    24781 cacgtcggga tcattatcgg tcataacggt gaagacttcc tggttgcaga aagccgggtt
    24841 cccctttcaa ccatcaccac gctatctcgt tttattaaac gttcgtctaa tcaacgctat
    24901 gctataaagc gattagacgc tgggctgaca gaacaacaaa aacaacgaat tgttgaacag
    24961 gttccctccc ggctacgcaa actttaccac acgggtttta aatacgaatc ttcgcgccag
    25021 ttctgttcaa aatttgtctt tgatatttat aaagaggcgc tatgtattcc ggtgggtgaa
    25081 atagagacgt ttggacaact gttaaatagc aatccgaatg caaaactcac tttctggaaa
    25141 ttctggttct taggttctat tccgtgggag cgtaaaaccg tcacgccagc cagtttgtgg
    25201 catcatccgg gtttggtgtt gattcacgcg gagggagttg aaacgcctca gccggaactg
    25261 accgaggcgg tataacttaa cgcagtcgcc ctctcgtcag gttcagccgc gattcgctca
    25321 tctgcatcgc attctgacta acgtggcagt gggtgatggc aatcgccagc gcatcggcgg
    25381 catccgcctg tggattagcg ggcagtttca gcaaggtgcg gaccatatgc tgcacctggc
    25441 ttttttcggc actacccata cctaccaccg tttgctttac ctgacgtgcc gcgtattcaa
    25501 ataccggcaa ttcctgattc accgccgcaa caatcgccac gccgcgcgcc tggcccagtt
    25561 tcagggctga gtcagcgttc ttcgccataa agacttgttc aatggcgaaa taatcaggct
    25621 ggaactgggt gatgatttcc gtcacgcccg catagatgag cttcagacga gacggtaaat
    25681 catccacttt ggtgcgtatg catccgctac ctaggtagga cagttgccta cctacctggc
    25741 ggatgacgcc gtagccggtc acgcgcgaac ccgggtcaat gccgagaata atagccatca
    25801 cgcgtctccg ttttgctgtt tagcaggcat catcagagag tcgctgcaac ctcatcagag
    25861 atttcaccgt tatggtaaac ttcctgcacg tcgtcgcaat cttccagcat atcgatcaga
    25921 cgcatcagtt tcggcgcggt ttctgcatcc atatcagctt tggtggacgg gatcatggaa
    25981 acttccgcgc tgtctgcttt cagacctgcc gcttccagag cgtcgcgcac tttacccatt
    26041 tcttcccatg cagtgtagac atcaatcgcg ccgtcatcat atgtcacaac gtcttcagca
    26101 cctgcttcca gagctgcttc catgatggtg tcttcgtcgc ctttctcgaa ggagatcacg
    26161 ccttttttgc tgaacagata agctacggaa ccgtcagtgc cgaggttacc gccacatttg
    26221 ctgaatgcgt gacgcacttc agcaacggta cggttgcggt tgtcagacag acattcaatc
    26281 atgattgccg tgccgccagg accgtaacct tcgtagatga tggtttccat gtttgcatca
    26341 tcatcaccgc ccacaccacg tgcaattgcg cggttcagtg tgtcacgggt catgttgtta
    26401 gacagtgctt tatccaccgc cgcacgcaga cgcgggttag cgtccggatc gccaccgccc
    26461 agcttagccg cggttaccag ctcacgaatg attttagtga agattttacc gcgcttagca
    26521 tcctgcgcag ctttacgatg tctggtgttg gcccatttac tatgacctgc cataaaaata
    26581 tctccagata gccctgcctg ttcaggcagc gttaattaca aactgttcaa tcgcctgccg
    26641 gttgctccag gacttagtga gcgccgccgc aacaggcgca tcaagccact tgtaagccag
    26701 atgttcagtg aaaacgatct gccgctcgtg cggaagcgca agacagaacc atgattccgt
    26761 attacgcgtc acgcccggcg catagcgatg acgtaaatgt gaaaaaattt caaactctac
    26821 cgtgcgctga cagtcaatta aggtcagttg ttcagcgaca acatcaatgg taacctcttc
    26881 ctttacttcg cgcatggcag cttgcggcgc ggtttcaccc tcttccacgc tgccggttac
    26941 cgactgccag aaatcgggat cgtcacgccg ctgcaacatc agcacccgtt tcgtatcttg
    27001 tgcatagatg accactaaga tcgaaacggg acgcttatac actttatcct tcacgctgcc
    27061 tcttcgttga ctgccttcgc tcatcccatt cacatagtta tctatgctca tgggagttca
    27121 ctcagttgcc gccttgatgc aacgcgaatg atttcgtgta tttgagtcat atcagttatt
    27181 ctcagccttc ttcacaacct gaatgctcag ctcagccagt gcagtcgggt tagcaaagct
    27241 cggtgcttca gtcatcagac acgctgccgc cgtggttttc gggaaggcga taacgtcacg
    27301 gatattgtcg gtgccagtca gcagcatggt cagacggtca agaccgaatg ccagacctgc
    27361 gtgcggcgga gtaccgtatt tcagggcgtc gagcaggaag ccgaatttct cgcgctgttc
    27421 ctcttcgttg atacccagaa taccaaacac cgtctgctgc atatcaccat tatggatacg
    27481 tactgaacca ccgcccactt cgtaaccatt gatgaccata tcgtaagcgt tcgccaccgc
    27541 attttccggt gcagctttca gttctgcagc agtcatatct ttcggtgagg tgaacggatg
    27601 gtgcattgcc gtcaggccgc cttcaccgtc gtcttcaaac atcgggaagt cgataaccca
    27661 cagcggtgcc catttgcttt cgtcggtcag accaaggtct ttacccactt tcaggagcag
    27721 tgcgcccatc ccgtcggcaa caattttctt gttgtcggca ccgaagaaaa tcatatcgcc
    27781 atcttgcgcg gcagtacgat ccaggatggc ttcgatgatt tctgcattaa ggaacttcgc
    27841 taccgggctg ttgatacctt ccagaccttt cgcgcgttcg ttaactttga tgtaagccag
    27901 acctttcgcg ccgtagattt taacgaagtt accgtattcg tcgatctgct tacgggtcag
    27961 cgatgcgcca cccggaacac gtagagcggc aacacggcct ttcggatcgt tcgccggccc
    28021 cgcaaatact gcaaactcaa cagatttcag cagatcggca acgtcggtca gttccatcgg
    28081 gttacgcaga tccggtttat cagaaccata acggcgttct gcttccgcaa aggtcattac
    28141 cgggaaatcg cccagatcca cgcccttcac ttccagccac aaatgacgca ccagcgcttc
    28201 catcacttca cgcacttgcg gcgcggtcat gaaagaagtt tccacatcga tctgagtaaa
    28261 ttcaggctga cgatcagcgc gcaggtcttc gtcacggaag catttaacga tctgatagta
    28321 acggtcaaaa ccggacatca tcagcagctg tttgaacaac tgtggggatt gcggcagtgc
    28381 gtagaattta cctttgtgca cacgagaagg caccaggtag tcacgcgcgc cttccggcgt
    28441 ggctttggtc agcatcggag tttcgatgtc gaggaagccg tggtcatcca taaaacggcg
    28501 caccaggctg gtgattttag cgcgggtttt caggcgctgc gccatttccg ggcgacgcag
    28561 gtcgaggtag cggtatttca gacgcgcttc ttcggtgttg acgtggttag agtcaagcgg
    28621 cagaacatct gcgcggttga tgatagtcag cgaggacgcc agcacttcaa tttcgccggt
    28681 cgccatatcg cggttaatat ttttttcgtc acgcgcacgt acggtgcccg taacctgaat
    28741 gcagaactca ttacgcagtt cagaggccag ctttaacgcg tccgcacgat ccggatcgaa
    28801 aaatacctgc acgatacctt cgcggtcgcg catatcgatg aagatcaggc taccaagatc
    28861 acgacgacgg ttgacccaac cacacagagt cacctgctgc cccacgtggg acaaacggag
    28921 ctgtccacaa tattctgtac gcatgagata tcccttaact tagctgccgg cggatgcccc
    28981 ctgctgcgca ggtgaccaag tcgcagcgtt agctgtatgt cacaactgaa tgaaaaaagg
    29041 cggctattat actggaaatt ctgccgcacc gtaagagcct ggcccgcgct ggaacgcctc
    29101 gttaccactt tatatcgggc ctgaaatcag actctacgcc agtttgctat aaaggtgttg
    29161 cccgaactca taaaaattaa caaaatttgt cgttccgcca tcggctaatc gcattaaggt
    29221 gtgaggcacg attttgtttt ttcaggagtc agcatgcttg aacttaatgc taaaaccact
    29281 gcgttggtgg tgatcgattt acaagaaggc atcttacctt ttgccggtgg tccacatact
    29341 gccgatgagg tagttaatcg cgccgggaag ctggcggcga aatttcgcgc cagcggtcag
    29401 cccgtgtttc tggtgcgagt tggctggtct gccgattacg ccgaagcatt aaaacagccg
    29461 gttgatgcc
//
LOCUS       DAOFMX010000052        29043 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.052, whole genome shotgun sequence.
ACCESSION   DAOFMX010000052 DAOFMX010000000
VERSION     DAOFMX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.052"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="QBA20_002545"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="QBA20_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDQ6293037.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPTTIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="QBA20_002546"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="QBA20_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDQ6293038.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="QBA20_002547"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="QBA20_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDQ6293039.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="QBA20_002548"
     CDS             4843..5082
                     /locus_tag="QBA20_002548"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293040.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="QBA20_002549"
     CDS             5092..5262
                     /locus_tag="QBA20_002549"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDQ6293041.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="QBA20_002550"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="QBA20_002550"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDQ6293042.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="QBA20_002551"
     CDS             6724..6990
                     /locus_tag="QBA20_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HDQ6293043.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="QBA20_002552"
     CDS             7084..7773
                     /locus_tag="QBA20_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDQ6293044.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="QBA20_002553"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="QBA20_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDQ6293045.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="QBA20_002554"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="QBA20_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDQ6293046.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="QBA20_002555"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="QBA20_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDQ6293047.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="QBA20_002556"
     CDS             complement(10475..12193)
                     /locus_tag="QBA20_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDQ6293048.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="QBA20_002557"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="QBA20_002557"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDQ6293049.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="QBA20_002558"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="QBA20_002558"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDQ6293050.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="QBA20_002559"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="QBA20_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDQ6293051.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="QBA20_002560"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="QBA20_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDQ6293052.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16273..18231
                     /gene="acs"
                     /locus_tag="QBA20_002561"
     CDS             16273..18231
                     /gene="acs"
                     /locus_tag="QBA20_002561"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDQ6293053.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18431..18745
                     /locus_tag="QBA20_002562"
     CDS             18431..18745
                     /locus_tag="QBA20_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDQ6293054.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18742..20391
                     /gene="actP"
                     /locus_tag="QBA20_002563"
     CDS             18742..20391
                     /gene="actP"
                     /locus_tag="QBA20_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDQ6293055.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20569..21861
                     /locus_tag="QBA20_002564"
     CDS             20569..21861
                     /locus_tag="QBA20_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDQ6293056.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22015..23664)
                     /locus_tag="QBA20_002565"
     CDS             complement(22015..23664)
                     /locus_tag="QBA20_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDQ6293057.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23815..25164)
                     /gene="ghxP"
                     /locus_tag="QBA20_002566"
                     /gene_synonym="yjcD"
     CDS             complement(23815..25164)
                     /gene="ghxP"
                     /locus_tag="QBA20_002566"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDQ6293058.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25870..26334)
                     /gene="soxR"
                     /locus_tag="QBA20_002567"
     CDS             complement(25870..26334)
                     /gene="soxR"
                     /locus_tag="QBA20_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDQ6293059.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26420..26743
                     /gene="soxS"
                     /locus_tag="QBA20_002568"
     CDS             26420..26743
                     /gene="soxS"
                     /locus_tag="QBA20_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDQ6293060.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26746..28323)
                     /gene="pdeC"
                     /locus_tag="QBA20_002569"
                     /pseudo
     CDS             complement(26746..28323)
                     /gene="pdeC"
                     /locus_tag="QBA20_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28752..29033
                     /locus_tag="QBA20_002570"
     CDS             28752..29033
                     /locus_tag="QBA20_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDQ6293061.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7289 a         7776 c         7347 g         6631 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 acaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ttcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggatcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttggcc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttattat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccaacaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcgatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcattct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtgaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaaaat taatcaactt ttgttggtga ccttcaaaaa
    16141 ttaccctgcc gtttatttgc accattctac ttttgcgtga tctgtcgccc aaatactaaa
    16201 caaaactgcc aataccccta catttaacgc ttatgccaca tattattaac atcctacaag
    16261 gagaacaaaa gcatgagcca aattcacaaa cacaccattc ctgccaacat cgcagaccgt
    16321 tgcctgataa accctcagca gtacgaggcg atgtatcaac aatctattaa cgtacctgat
    16381 accttctggg gcgaacaggg aaaaattctc gactggatca aaccgtacca gaaggtgaaa
    16441 aatacctcct ttgcccccgg taatgtgtcc attaaatggt acgaggacgg cacgctgaat
    16501 ctggcggcaa actgccttga ccgccatctg caagaaaacg gcgatcgcac cgccatcatc
    16561 tgggaaggcg acgacgccag ccagagcaaa catatcagct ataaagagct gcaccgcgac
    16621 gtctgccgct tcgccaatac cctgctcgag ctgggcatta aaaaaggtga tgtggtggcg
    16681 atttatatgc cgatggtgcc ggaagccgcg gttgcgatgc tggcctgcgc ccgcattggc
    16741 gcgatgcatt cggtgatttt cggcggcttc tcgccggaag ccgttgccgg gcgcattatt
    16801 gattccaact cacgactggt gatcacttcc gacgaaggcg tgcgcgccgg gcgtagtatt
    16861 ccgctgaaga aaaacgttga tgacgcacta aaaaacccga acgtcaccag cgtagagcat
    16921 gtggtggtac tgaagcgtac tggcggaaac atcgactggc aggaagggcg cgacctgtgg
    16981 tggcacgacc tggttgagca agccagcgat cagcaccagg cggaagagat gaacgccgaa
    17041 gatccgctgt ttattctcta tacctccggt tctaccggaa aaccaaaagg cgtactgcac
    17101 actaccggcg gttatctggt gtacgcggcg ctgaccttta aatatgtctt tgattatcat
    17161 ccgggtgata tctactggtg caccgccgat gtgggctggg ttaccggaca cagttatttg
    17221 ctgtacggcc cgctggcctg cggcgcgacc acgctgatgt ttgaaggcgt accgaactgg
    17281 ccgacgcctg cccgtatggc gcaggtggtg gacaagcatc aggtcaatat tctctatacc
    17341 gcacccacgg cgatccgcgc gctgatggcg gaaggcgata aagcgattga aggcaccgac
    17401 cgttcgtcgc tacgcattct cggttccgtg ggcgagccaa ttaacccgga agcgtgggag
    17461 tggtactgga aaaaaatcgg caacgagaaa tgtccggtgg tcgatacctg gtggcagacc
    17521 gaaaccggcg gtttcatgat caccccgctg cctggcgcta ccgagctgaa agccggttcg
    17581 gcaacacgtc cgttcttcgg cgtgcaaccg gcgctggtcg ataacgaagg taacccgctg
    17641 gaaggggcta ccgaaggtag cctggtgatc accgactcct ggcctggtca ggcgcgtacg
    17701 ctgtttggcg atcacgaacg ttttgagcag acctattttt ccaccttcaa aaatatgtat
    17761 ttcagcggcg acggcgcgcg tcgcgatgaa gatggctatt actggatcac cgggcgtgtg
    17821 gacgacgtgc tgaacgtctc cggtcaccgt ctggggacgg cagagataga gtcggcgctg
    17881 gtggcgcatc cgaagattgc cgaagccgcc gtagtaggta ttccgcacaa tattaaaggt
    17941 caggcgatct acgcctatgt cacgcttaat cacggggagg aaccgtcacc agaactgtac
    18001 gcagaagtcc gcaactgggt gcgtaaagag attggcccgc tggcgacgcc agacgtgctg
    18061 cactggaccg actccctgcc taaaacccgc tccggcaaaa ttatgcgccg tattctgcgc
    18121 aaaattgcgg cgggcgatac cagcaaccta ggcgatacct cgacgcttgc cgatcctggc
    18181 gtggtcgaga agctgcttga agagaagcag gctatcgcga tgccatcgta acccacaatt
    18241 gccggatgcg aatcggtaac ggtttgtagg cctgataaga cgcgacagcg tcgcatcagg
    18301 cattgattgc cggatacggc gtaaacgcct tatccggcct acattcggca agggttaccc
    18361 gagcgttaac cttctcccat aagggagcgg gaattaaaac aatccctaca ttacctctgg
    18421 agaatctgtg atgaatggta cgatttatca gcggatagaa gacaatgcgc atttcaggga
    18481 gttagtcgaa aaacggcaac ggtttgccac catcctgtcg attattatgc tggcagttta
    18541 tatcggcttt attttactga tcgccttcgc gcccggctgg ctgggcacgc cgctgaatcc
    18601 gaacaccagc gtcacacgcg gtattccaat tggtgttgga gtgattgtga tctcctttgt
    18661 tctcaccggt atctacatct ggcgggcgaa cggcgaattc gaccgtctta ataatgaagt
    18721 cctgcatgag gtacaagcat catgaaaaga gttctgacgg cgcttgccgc cacactccct
    18781 ttcgcagcta acgccgcgga tgctattagc ggggccgtag agcgccagcc aacgaactgg
    18841 caggcgatta ttatgttcct gattttcgtc gtgtttacgc tcggcattac ctactgggca
    18901 tcaaaacgcg tacgttctcg tagcgactac tacaccgcag gcggcaatat cactggcttc
    18961 cagaacgggc tggcgattgc cggggactat atgtccgccg cctcattctt ggggatctcc
    19021 gcgctggtgt ttacctccgg ctatgacggg ctgatctact cgttgggctt cctggtgggc
    19081 tggccaatca ttctgtttct gattgccgaa cgtctgcgta acctgggacg ctacacctct
    19141 gccgatgtgg cctcttatcg tctgaaacaa gggccgattc gtattctttc ggcctgtggt
    19201 tctctggtag tggtggcgct ttaccttatc gctcagatgg taggcgcagg taaactgatc
    19261 gagctgctgt ttggccttaa ctatcacatt gcggtggtgc tggtcggcgt gctgatgatg
    19321 atgtacgtcc tgttcggcgg catgctggcg accacctggg tacaaattat caaagctgtg
    19381 ctgttgctgt tcggtgccag ctttatggcc tttatggtga tgaaacacgt cggctttagc
    19441 ttcaacaatc tgttcagcga agcgatggcg gtacacccga aaggtgtcga catcatgaaa
    19501 ccgggcggac tggtgaaaga tccgatctcc gcgctctctc tggggctggg actgatgttt
    19561 ggtacggcgg gcttgccgca cattctgatg cgcttcttta cagtcagcga tgcccgcgaa
    19621 gcacgtaaga gcgtgttcta cgccaccgga tttatgggct acttctatat tctgaccttt
    19681 attatcggct tcggcgcgat catgcttgtt ggtgcgaatc cggaatataa agacgcggcg
    19741 ggccatctga ttggtggtaa caacatggcg gccgttcacc tggcgaatgc agtgggcggc
    19801 aacctattcc tcggttttat ttcagcggtt gctttcgcca ctatcctcgc ggtggttgcg
    19861 gatctgacgc tggcgggcgc atcggcggtt tcgcatgact tgtacgctaa cgtcttcaaa
    19921 aaaggcgcga ccgaacgtga agagctgcgg gtatcaaaaa tcaccgtact gatcctcggc
    19981 gtgattgcga ttatcctcgg cgtgctgttt gagaatcaga acatcgcctt tatggtaggc
    20041 cttgcgtttg ccatcgcggc gagctgtaac ttcccgatca ttctgctttc catgtactgg
    20101 tcgaaactga ctacccgtgg cgcgatgctg ggcggctggc tggggctgat taccgcagtg
    20161 gtgctgatga tcctcggccc gactatttgg gtacagatcc tcggtcacga aaaagccatc
    20221 ttcccgtatg aatacccggc gctgttctct atcagcgtgg cattcctcgg catctggttc
    20281 ttctcggcaa ccgataactc agcggaaggg gcgcgcgagc gtgaactgtt ccgcgcgcag
    20341 tttatccgct cccagaccgg ctttggcgtt gagcaaggcc gcgcacatta atctctcccc
    20401 ttccccggtc gcctgaccgg ggaatacgct tcctctccaa catgcatcac cttttcccat
    20461 aatattaaac aaataaactc attaaaaaat gagcgatttt tgacagtcgt agaaaatgat
    20521 aatgcagaga atatgccttt tctttcttgt taattataag gatattttat gcgctataat
    20581 ggtttaaata atatgttttt ccctctttgc cagattaacg ataaccactc tatcacaagt
    20641 tcatcacata caaagaaaac aaaatcagat aattacagca aacatcataa aaacacgtta
    20701 attgacaata aagccctctc tcttttcaaa atagatgatc atgaaaaagt gataggcttg
    20761 attcagaaaa tgaaaagaat ttatgatagt ttaccatcag gaaaaatcac gaaagaaacg
    20821 gacaggaaaa tacataaata ttttatagat atagcgttat atgcaaataa taaatgtgac
    20881 gatagaatta cgagaagagt ttaccttagt aaagaaaagg atgtatccat taaggtggta
    20941 tattttataa ataatgtcgc cgtccataat aatactatcg aaattccaca gatagtaaat
    21001 ggtggttacg atttttcaca ccttagcctg aagggtatcg tgattaaaga tgaagattta
    21061 tccaattcga attttgcagg ttgcagacta caaaacgcta ttttccagga ctgtaatatg
    21121 tataaaacga atttttatta cgccataatg gaaaaaatac tttttgataa ttgtattctc
    21181 gatgactcaa atttcgctca gataaaaatg gccgacggaa ctctaaatgc atgctccgct
    21241 atgcatgttc aattctacaa tgcagcaatg aatagagcca atattaaaaa taccttcctt
    21301 gactattcaa atttttatat ggcgtacatg gctgaggtaa atctttataa agtaatagcg
    21361 ccatatgtta atttatttaa agccgacctt agtttctcta aactcgattt aattaacttt
    21421 gaacatgctg atctgtctcg cgtcaatctg aacaaagcaa tcctccagaa tataaactta
    21481 attgatagca aactcttttg tacgtggctg acaaatacat tcctcgaaat ggttatatgt
    21541 accggctcta atatggctaa tgttaatttt aataatgcca atttaagcaa ctgccatttc
    21601 aactgttcta ttttaacaaa agcctgtatg tttaataccc gtctctatcg ggttaatttc
    21661 gatgaggcta gcgtccaggg aatgggcatt tccattctcc gtggggagga aaatatcccc
    21721 attgatagtg ataccctggt aacactacag aaattctttg aagaagattg tacctctcat
    21781 actggcatgt cacaaactga ggataatatt aatgcagtcg ctatgaagat tactgcagat
    21841 attatgcaac acgcagattg aggttaagtc gaagaacagc caatgaattg actttacaag
    21901 agagataact tatttgtttt agaatcatga atgttcgccc gagctaacct gttaaaggtg
    21961 caaaagattg cacctttttc agattacctc gggcctggtt acgtcgcttt cggcttactg
    22021 attttcctca attagcaacg cttcaagcaa atcgagatcg tgcagtaatt tttgcagcgt
    22081 ttcgttgctg atctcccgcg tggcgcgcag gtggtacagt tcagcacgtt cagaacgcaa
    22141 tgccgccaga cggaagcgac gctcaaggtt ctcttcctgc acggaacttt caacgtcgtt
    22201 acgtccatcg gcgcgacgac gcaggttacc aatgacgcga gaactgacct ccgtaagcaa
    22261 ctggttatcg atattctctt cggtatccgc cgccagacgc tcctccattt tctggatcgc
    22321 cacaatcgcc acttctgccg ttgccgctcg cgcaatacgt tcttctttca attgttgcga
    22381 atggtccgcg acttcaatgt gttgtagcag aataggcaac atcaccacgc cgacaaacag
    22441 cgagaagaga atgacgccag ccgccaggaa caccagctca tagcgcgccg ggaagacgtt
    22501 accatccggc aagagcagcg ggattgagag cacaccggcc agagtgatcg ccccacgcac
    22561 cccagcgaaa gacgcgatca ggatttctcg tgtggtccac gaaccaaact ccatcggctt
    22621 cttcttcagg aagcggttgc tgaacttttt catcgtccac agccagccga aacggaccag
    22681 catcagcgcc gcatatatca gaataatatc ggtaaacagc atccagattt cgacgttagg
    22741 gtcgatttct gccgccatca gcgacgtctc cagaataccc ggcagttgca gacctaacag
    22801 caggaacacc atgccgttaa agacaaattc cagcatcgcc caggtgctgt ttgcgcgcag
    22861 gcgcattgcc agtggcgcac ggcgcatcac accggagcgg gtgatggtca tcccggcggc
    22921 aaccgcagcg aggatgcccg aaacgccaat atgttcggca atcagataag aagcgaatgg
    22981 cagcaagaac agcagaacga tctgcgtcgc gggttcatca ccgccccagc ggctgaggaa
    23041 tcgcagcgag cgaccataca gccagctcac cacaaaaccg gcaagaatac cgccaatggc
    23101 tactttcata aattcgaccg tcgcaccgcc gacagtgaag atcatcgtcc ccatcgccac
    23161 tgccacggca aacttcaacg acaccaggcc ggaagcgtca ttcatcaacg cttcgccctg
    23221 caaaatgccc atgatttttt tcgggatgcg cccttcgcca acaatcccgg agagcgccac
    23281 agcatcggtc ggagaaagta ccgccgccag cgcaaaggcg gggatcagcg gaatgcccgg
    23341 caccacccag taaataagga agccaatgcc gaccacggtg accaccacca gcgccagcgc
    23401 gaggccgaaa atctctcgac catgttcgag aaattcacgg gtcggcgttt tccagccatc
    23461 agcgaacagc aacggcggga taaacaagac taaaaagagt tcaggatcaa actccacatg
    23521 caaaccaaac gtcggccacg ccagtagcgc accgatggcg atttgcataa gcggaagcgg
    23581 gatctgaaag ggcatgacac gagtgaccac cccggacagc gagaccacga gggtcattat
    23641 cagtatggtg aagaagattt ccatgcgttc cctgtttgcg atgtttttta tgatctgaga
    23701 aagacgttat gcctgaattc tcattttttc agaatatcgc aaaagctcac acaatgtgcg
    23761 gagaaaataa aaacgggcag ccattgccac ccgttttcag aatccgatga aagattagat
    23821 agcccagcca cccgcataga aggtcaccag cgccacggcg ataacgaccg taccgatgtt
    23881 caacttgcgc cattcaccgg aaaccagacg accaatcacc agagtcgcga agccgatcat
    23941 gatgcctgtt acgatgttac aggtcagcac gatgaatacc gccgtaacca gacccgccat
    24001 cgcatcaaca aaatcagcaa agtcgatttt cgccacgttg ctcagcatca gcaggccaac
    24061 gtacatcagc gccggagccg ttgcataccc cggaacgagg taagaaagcg gagagaggaa
    24121 cagaatcagc aggaacagca cgccaacggt gatagccgtc aacccggttt taccgcccgc
    24181 cgccgtaccc gccgcagact cgatgtatac cgctgccgga gctgcaccca ccaggccaga
    24241 gaaaacgctg ctcatggagt cagtggtcag tgctttcccg ccgtcgatga tctgcccatc
    24301 tttatccagc aggttcgcct ggccggcgac agcgcggata gttccggtgg catcaaatac
    24361 tgccgtcatc accagcgcca gaacgcttgg caggactaca ggattcagcg cgcccataat
    24421 gtccaggctg ccaatcagtg aattgccgtt ttcatcgctc aatgaaggca tggcgaaaac
    24481 gccggagaaa tggacgttag gatcgaagat caaaccgaca attgagatac cgataatggt
    24541 cagcagaatg ccaccaggga ctttcagttt ttccaagccg atgatcaccg ccagacctac
    24601 cagtgacata atcaccggga aggtcgcgaa atcacccagc gcaacgggca gaccatcaag
    24661 cgggttttta atcaccagac cgacaccatt agcggcaatg agcagcagga acagaccgat
    24721 accgatcccc gtgccgtgcg ccacaccgtg cggcaagttg cgcaaaatcc agctacggat
    24781 acccgtggca gaaattaccg taaacagcac acccatcagg aacacggcac ccagcgcgac
    24841 aggtacgcta atatgttgcc ccagcaccag gctgaatgcg gtaaacgccg tcagggagat
    24901 ggcgcaacca atcgccaacg gcagattagc ccacagaccc atcacgatag aaccgagtcc
    24961 ggcaaccaga caggttgcaa cgaaaactgc cgcaggcggg aagcctgctt tacccaacat
    25021 acctggaacg acgatgaccg agtagaccat cgccagaaac gttgttaacc cggcaaccac
    25081 ttcctgacgg acagtgcttc cacgttgtga aattttaaac caggcgtcga gtgaaccgcc
    25141 ggtacgcgct gatggcgtag acataagaaa acatcccctg agagtttaat tttcgtcaag
    25201 atgcgtggcg gacaatcccc tgccagcgaa acgttatctt cctgctccag gtttgcggca
    25261 atttggtgcg tcgcaaaaaa gcaaacgttt agctccgcgc atacaaaacg ggtggtaaaa
    25321 aaaagcaaac gattatccgg cgatcatagc ggctttttca accgaagttt gctgcttttt
    25381 tctcttttat tgtcttgacg atgaaaaacc aggcaattga tgcgcaaacg ttatcgtcga
    25441 tgtgcaagat tgcagcgtta tcgagccgta taccgttctg gattgcgata aaaaatcgtc
    25501 tgagatgaca aaaatattgc ggctaaaaag tatactttta cactggcgaa gaaacgtcgg
    25561 tgacatacaa ttaaagcatc aacaccaacc ggaacctcca ccacgtgctc gaatgaggtg
    25621 tgttgacgtc gggggaaacc ctcctgtgtt ccagcgggat agagagaaag acaaagaccg
    25681 gaaaacaaac taaagcgccc ttgtggcgct ttagttttgt gtttgcggat aattccaaaa
    25741 gagctcgatc agtcccctcg ccccttcggg gagagggtta gggtgagggg aacaggcagc
    25801 accagtgcaa atatttaccc tcaccccggc cccatagtta acgatgagtc agcttgtcct
    25861 tgtggcgctt tagttttgtt catcttccag caagcgtgcg ccggtacctt cttctcctaa
    25921 gcggtcgccc gggttacgca acgggcaatc actgcgcgaa aggcagccac aaccaataca
    25981 tccgtccagt tcgtcacgca gcgccactaa ggtatgaatg cgccgatcca actcttctcg
    26041 ccattgggac gaaagctgtt tccactcttt cgcacttaac gtatgccctt ctggcaacac
    26101 gccaaacgct tcaccaatgg tcgccagcgg aatgccaata cgctgagcga ttttgataat
    26161 tgcgacatat cgcaacacat cacgtttata tcgccgctga ttgccgctgt tacggatact
    26221 agtaatcaac cctttgcttt catagaaatg cagcgccgat accgccacac cgctgcgttt
    26281 cgccacttcg ccaggggtta gcagcgcttt aatgcggggt aatttctttt ccataaatcg
    26341 ctttacctca agttaacttg aggaattata ctccccaaca gatgaattaa cgaactgaac
    26401 actgaaaaga ggcagattta tgtcccatca gaaaattatt caggatctta tcgcatggat
    26461 tgacgtgcat attgaccagc cgcttaacat tgatgtcgtc gcaaaaaaat caggctattc
    26521 aaagtggtac ttgcaacgga tgttccgcac ggtgacgcat cagacgcttg gcgattacat
    26581 tcgccaacgc cgcctgttac tggccgccgt tgagttgcgc accaccgagc gtccgatttt
    26641 tgatatcgca atggacctgg gttatgtctc gcagcagacc ttctcccgcg ttttccgtcg
    26701 gcagtttgat cgcactccca gcgattatcg ccaccgcctg tagttttatt gcccgcgcgt
    26761 taactcccgc gcgggtaatt gctccatcca ttgcataaac acctgcggcg gcatcgcctt
    26821 cgcaaaaaac catccctggc aatagcgcac gccgcgtttg cgcagccagt taacctgctc
    26881 ctcagtttcg acgccttcag cgatcgtttt taaccccagg ctgtgcgcca gctcgatgat
    26941 gtgttccgca atcaaatgac tggttttgtg ggtggtcagc gtttcaacaa acgatttatc
    27001 gattttcaaa atatcgacat tcaatgattt aaggttatgc aagttagagt agccaatacc
    27061 aaaatcatca attgccactt cgtaaccagc ctggcggaaa gccagaatta atcggcgtca
    27121 ttttttcgac atcaagaaat gcatgctcag tcacttcaaa tttaatttgc tgcggacgca
    27181 ccgcgtattg ctctgttttc tgattgattc gcgctatcaa ccgtgacgta tgaaaatcgg
    27241 aggccgacag gttaatagaa acatagcgat cggcatgtgt tgccaggtac gcgcccagat
    27301 cgcggaagac attatcaata acataatcag ttatctgttc tatcatcccc tccttttctg
    27361 ccagcggaat aaactctgcc ggattcatta tttgcccctg ctcaccaggc caacgtaaca
    27421 acgcttcagc gccgatacat ttttctgttt tgatatcgat tattggctgg taataaagac
    27481 aaagttgatg tttttcgagg gcgcgttgca atttacgttt gggagataaa tagttttgtc
    27541 gaatacgtag ccagagtaat agcagaacaa gactccccaa aataccggcg ggcaacgcaa
    27601 aaataagatg attataaaaa tgggttataa gacgttgata tgaagtcgca acaatggctg
    27661 caattgggcg ttttgtcgaa taaattgtcg catataaata gccatttctt tgtacggtta
    27721 aatccttcaa atgaatcagc ggagaaaacg ttgctgccga ggcctctttg cttaacgaga
    27781 aaaaggtttt cgtcaccgta tcatacacac cccattgcaa tgtcgggtca tcagacatca
    27841 cttcactcca gaagagaggg ttgataaccg ccacataatt tccccgctgc atataggtca
    27901 ttttatagcc agagaaaaaa ggcgtatcgc ggtaataata gatagaaacg ttaggttcac
    27961 gcttataatc ggccggtgca atcgtatagc cgtttacagg cgctatcagc gatgagcata
    28021 aaaaatggtt atcacgaatt cattaatata aagatagcca cgaataatat tcaacattcg
    28081 cttttgatgg gctggagtgc aaacttgccc ctgatagcgt tcagctgcat cgctcactaa
    28141 atctgcctgg cgaatgacca gctcggattt atccagtgcc agttgagcaa aggttcggag
    28201 ctgattattc acttctgatt ttgcccaaag gaaggcaatc catagcgaaa gaatgatggg
    28261 aaagagaact aaaaagataa tgcccggcaa cgccagtaat tggtgtcgtg cacgatgact
    28321 cattgctatc aatttcctgc ctgtttatac ccggccattg caaggtgaaa ccctggctac
    28381 cgacgtcttt agataaatct tacggtataa atagcacttt tgcctgattt gaaattgata
    28441 cctttttaat cgtcacctgg atgaaaataa ttcattgata ttcaaaatta attttctaca
    28501 ggaatacaga ggcggcggga agatatgccc ttaatgccac tgaaaaacca cataatttgt
    28561 acagcaataa aagtcacggc ctaatatggc taacaaacaa tcaattgtga tatagttcac
    28621 aaaattaatg aaacaaacag agtgtttcat ttttgtgttc tgcaagcccc gcgatagcca
    28681 gagtgtacca cgcctcccgt gaacaacgcc gcgctgtcca gggcatcggc tcttttcaga
    28741 ggaattgttt gatggctacc ctcaccactg gcgtggttct tcttcgctgg caacttctta
    28801 gtgccgtaat gatgtttctg gccagcacac tcaacatccg ttttcgtcgg tctgattatg
    28861 tcgggcttgc ggtgatcagc agcggtctgg gcgttgtttc cgcctgttgg ttcgcaatgg
    28921 ggttgcttgg catcacaatg gcggacatca ccgctatctg gcacaacatc gagtcggtga
    28981 tgatagaaga gatgaatcag acaccgccac aatggccaat gattttgacc tgatacgtag
    29041 aag
//
LOCUS       DAOFMX010000053        28928 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.053, whole genome shotgun sequence.
ACCESSION   DAOFMX010000053 DAOFMX010000000
VERSION     DAOFMX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.053"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="QBA20_002571"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="QBA20_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDQ6293062.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGELLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="QBA20_002572"
     CDS             complement(2138..2584)
                     /locus_tag="QBA20_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDQ6293063.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="QBA20_002573"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="QBA20_002573"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDQ6293064.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="QBA20_002574"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="QBA20_002574"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDQ6293065.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="QBA20_002575"
     CDS             complement(5347..6009)
                     /locus_tag="QBA20_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDQ6293066.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="QBA20_002576"
     CDS             6182..6595
                     /locus_tag="QBA20_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDQ6293067.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="QBA20_002577"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="QBA20_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDQ6293068.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="QBA20_002578"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="QBA20_002578"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDQ6293069.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="QBA20_002579"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="QBA20_002579"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDQ6293070.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="QBA20_002580"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="QBA20_002580"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDQ6293071.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="QBA20_002581"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="QBA20_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDQ6293072.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="QBA20_002582"
     tRNA            complement(9861..9948)
                     /locus_tag="QBA20_002582"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="QBA20_002583"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="QBA20_002583"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDQ6293073.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="QBA20_002584"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="QBA20_002584"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDQ6293074.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="QBA20_002585"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="QBA20_002585"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDQ6293075.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="QBA20_002586"
     CDS             14005..14592
                     /locus_tag="QBA20_002586"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDQ6293076.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="QBA20_002587"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="QBA20_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDQ6293077.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="QBA20_002588"
     CDS             14984..15841
                     /locus_tag="QBA20_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDQ6293078.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="QBA20_002589"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="QBA20_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDQ6293079.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="QBA20_002590"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="QBA20_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDQ6293080.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="QBA20_002591"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="QBA20_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDQ6293081.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="QBA20_002592"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="QBA20_002592"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDQ6293082.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22444)
                     /gene="etk"
                     /locus_tag="QBA20_002593"
     CDS             complement(20264..22444)
                     /gene="etk"
                     /locus_tag="QBA20_002593"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HDQ6293083.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22464..22910)
                     /gene="etp"
                     /locus_tag="QBA20_002594"
                     /gene_synonym="yccY"
     CDS             complement(22464..22910)
                     /gene="etp"
                     /locus_tag="QBA20_002594"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HDQ6293084.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22898..24037)
                     /locus_tag="QBA20_002595"
     CDS             complement(22898..24037)
                     /locus_tag="QBA20_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDQ6293085.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24083..26179)
                     /locus_tag="QBA20_002596"
     CDS             complement(24083..26179)
                     /locus_tag="QBA20_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDQ6293086.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QPYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26179..26925)
                     /locus_tag="QBA20_002597"
     CDS             complement(26179..26925)
                     /locus_tag="QBA20_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDQ6293087.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26922..27566)
                     /locus_tag="QBA20_002598"
     CDS             complement(26922..27566)
                     /locus_tag="QBA20_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDQ6293088.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27673..27978)
                     /locus_tag="QBA20_002599"
     CDS             complement(27673..27978)
                     /locus_tag="QBA20_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293089.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28420..28632)
                     /gene="cspH"
                     /locus_tag="QBA20_002600"
     CDS             complement(28420..28632)
                     /gene="cspH"
                     /locus_tag="QBA20_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDQ6293090.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7004 a         7332 c         7606 g         6986 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aattcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg
    22321 tgaataacgc ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac
    22381 gaagcagatc gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg
    22441 tcatcgtatt ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg
    22501 cgctccaaca taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg
    22561 atctctttct gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca
    22621 attgcggtaa cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt
    22681 gccatctcag cagtgagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg
    22741 gcaacatctg ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat
    22801 ttgactttta cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa
    22861 atgttgccgg tacaaaccac taggattgag ttaaatttta gttgggccat gtttgaatcc
    22921 accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca
    22981 cacggttcca acgagccagc ggcgcggtag tgacataaac aatgtcgtaa ggttcaagct
    23041 ggaattccgt tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg
    23101 cgattttgcc attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag
    23161 cgacatcctg attcataccc tcagcgttac ccagcgcttc tgccagggtc ataccgctgc
    23221 ggtccatttt cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat
    23281 cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc
    23341 cacgctgcat cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca
    23401 cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg
    23461 tcagaggaat attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg
    23521 ctttttgcga tcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat
    23581 cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc
    23641 gaccaatata ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg
    23701 cgctacggta ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca
    23761 acacatcacc aatcccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt
    23821 tagcctgcga cataatcggt ttggcgcgca gctgatcaat caatcctggc gtgacgggat
    23881 agatattgac cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt
    23941 tactggtcga taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca
    24001 atgtcagtac cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg
    24061 taatgttatg aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg
    24121 gatcgagctg gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg
    24181 aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa gtcgaacgga atcgagatat
    24241 aaaaaccttt actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg
    24301 cccatacccc taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt
    24361 tatcttttgc cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg
    24421 ggttccagta agccgtcacg aaacctgtgg gcgtggaata atcggtgaag cgcatcatgt
    24481 tgtcccagtc acgctgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta
    24541 gcgggcgata aagaagctcg gaaccgacac cggcatacat cgtttccaga taaccgccat
    24601 acacctggcc atagaaacca ttgcctaagt cggcaaagta attcgcttgc aagttgttga
    24661 gataaacgtc attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt
    24721 ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac
    24781 cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc gctggacatc agccccagct
    24841 ggaacatata gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt
    24901 agctaaagcg atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt
    24961 caacacgttg ttgttgcagt tgctctgatt taccaggcgc tgtgccggcc agctgtttgc
    25021 gcaggctggc tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca
    25081 cgctaatctt ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga
    25141 ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga catatacagc gtcttatcgc
    25201 gtagctgaat ttctggcgcg tcaaagcccg cgttatactt cagggcggta agttggtttg
    25261 ctaccgtggt gtactgcaaa ccttcagact ctggcgcagg ctgccaggcc ggttttggcg
    25321 tatcgcgtag ggcagggcgc agatcgttga aattggtccg taaagtgaaa ccaaacatca
    25381 acgtgtttcc gcgttcataa ctgaggttga ggtctgccca gctggcagcg cgataaactg
    25441 caccgacgtt gaaatggctt gcctgaggca gtttgccagc gaaatcattc tggtaattgt
    25501 tgccgtcgta ttcgagtttc agacgcaggg gattccacgg cgtttgatac tcaatgccgc
    25561 caaagatgga agccgggcca cgaaagatat cgctaaagct gatatcgccc gcatcgtgag
    25621 actctgcgcg atgacaatat ttatcgctca cccggcaaaa cgggttggta atattgcccg
    25681 cattaccggc gtagccccat gccatcccga gggtgaaatc aaatggcccc gcttgcttgc
    25741 tggccaccag atattcaccg tcaaacaaac ccgtaccagc aatatcacgt ttaccaaacg
    25801 ccacttgcgg tagccagtaa ccttcttccc acaggcgaag tttaaaatcg aatgatttgt
    25861 ctttgtatgg ctgatcgccg ctgaaatctt cccactggct atatttgcgt gtgcgcacat
    25921 ccgtataacg aatggtgcct tccagccatg ggaacagcgc cacggaggtg gagtagaacc
    25981 ggtattgatc gttatcccgg tagttgacgc tgaattcacc ttccggtgcg atgcgcgcat
    26041 ttggcatctg caacaatcct gtgccgccaa agtctgattg cgacgaaccc agcggatccg
    26101 ggtaggttaa tacttcagca tgacaggcac tggagacggc aatggccagg cagcttaaaa
    26161 gataagaatt cttcttcatc agtcaggaac acgttgcgtt aaaacagaca caatctggtc
    26221 atttaaatca gcatattttt cgggcagtac atgcgcagaa aagcccaacc aaagctggct
    26281 gccaggcggt ggttccacat gtcttttatt ccacagggca acaggggcga cgacagtctc
    26341 accctcaggc gtaatcacca taacgttatt cttgtctgcg cctgcaaggc gggggtgatc
    26401 ttgcagataa tcggtaacgc tacggcctgc ctgccagggc aattgcccag ccccagaaac
    26461 cgcacccagc agagtaatag tcaccggacg ctgtacggta tagagcgtat agtccccgac
    26521 caacggggga ttgctgttct catccactcg tacaaaatca gggtccagtt tgaccggtaa
    26581 gcgaccagtg atattgagat tgagtaactg ctggcgcacg gatttaatcg ttgccgccac
    26641 atcgtcgtca gcttctgctt cccaggaagc aagctgtgcc atgacatgtt ggtagtcttt
    26701 aagcgcttta gctttcgccg cagagtcggt cagcaaggca ccaggccacc agaggcgatc
    26761 gcgcagttgc ggttgtgtca ccaactggac aacattttcc acggggccaa cggaaagtgt
    26821 ctgttgttcg ctaggcagat aaatagtcac cgttccttgc gcaaaggcat ggggtgtcat
    26881 tacgtaaagt acgctggcaa tgaaatacga ctgtaattta ttcatggtgc gggtttaaga
    26941 aacgtcattt ctacgggaat aacccctgcg cctaacattt gccgactttg acgaacctgg
    27001 ccagtagccg aatcgaccca gaatgtgttt tgccactgtt tttctggcag cgtggaagtc
    27061 acctcttcct gccagacatt acacagcaca ttgtgacccg caatattcag cgtctctaat
    27121 ccagcaaagg aaaaagtaga acttagggtc gcagagcgaa aatggttgtc ttcactccag
    27181 agaatatcgc gcgtccagcg gctgccatct tttattgcca gcgcgttacg cagaggggcc
    27241 cgcccgggat ttgtcacttc cagcagatta ttattaagtt tgacggtctt taaaagacgt
    27301 ccattgtggg taaccagcat ggcgttatcc tgggataacc atttgctttg ttcttgttct
    27361 atatatccca gtaccacaaa gattcgttgc ccttcattaa ggcgtaaata catcgtggaa
    27421 taaggcaacg cctggatctg ctgagccgct acagtgatat cctgattatc gaaaaggctg
    27481 gcgcgaaatg tatcgaccat actttgctgg ctgtgcgtac atcccgcaag aaatagtgcg
    27541 aaaatcgata aaataagagg gcgcaccttt aatccttgaa aggttaatta aaaaaatgca
    27601 aatctctaaa gcccggtacg aatatcgcaa ccgggtatta tttaacttga atttcgacgc
    27661 gtttaagcag aattagcgcg tagtggacgt ggtggttgag gtgttatgag aaccgccatc
    27721 gccactgtca tttgctgcaa ctactgccaa cgtcgccaca ccaacagccg ttgccacacc
    27781 gacaactgtc gccgtgccga cagcgccagc agccacgcca ctagtggtag ccgcagtcgt
    27841 ggttgcacct gtagtggtcg ttgtggtgcc agtagtaccc gtcgttgcct cggtcccgtt
    27901 tgctgcttca ggggcagcaa acgcagctgg cgtcgtcaac atgaaggcca taaggattgc
    27961 agaaagcttg tgtttcataa cttttccttt attcatcgca tggacaatac gtacgcttga
    28021 gatcaggatt gtctactttg agtataacat ccttgtgaaa tctccctttc ctcataccgc
    28081 tagcactata tactgcgggt gggaaaggcg cgcagaggga aatataagat tgttaacgat
    28141 ttccctctgg aaatacgact tgtgagcata agataaatct tagaaaaata cgaattaaag
    28201 ataagaaaat tgttacgaaa gctattaatt attgttggta atatagtttc aagtggtact
    28261 ataactggct gttaaatggc atttataact attaggtgca gagatattcg cttaaatgga
    28321 ttagtttctt agtggaaaag taactgatgt tataatgtat ggtgttgact attgaactat
    28381 tccaataaaa ccagtcagct ttaaacaagc agcgtcatat taagagagat aaacatttgc
    28441 cgctgttggt cctcgcaggc catttacgcg gcaaaattcc acgcgtaatc ctggtataag
    28501 cacttctgcg tcgcggggag tgaatgcgga aatatggacc tggacttctt tacgaccgtc
    28561 ggaggggata atgaatcctt tgccgctttt gcgatcaaag gttttgacaa ttcctgtcat
    28621 tttacgggac aaacaaattc cttactgaaa atactgcgct gcactatacg gggttaataa
    28681 aataaagcca gcgatattta agaccgccgg acggctaaaa taaaatttgc ttaatctcaa
    28741 ttatcatgcc ttaatagctg cgtcggtttg aaagacagac agcatacaaa gtagtttact
    28801 aaagcagttc tcattatcag gcattatccc cttcttttga gtctctctcc tgaacactaa
    28861 gtggtttctg tattaaagcc ctgtttgccg aaaggcccaa aatgaaggaa gtaaaatatg
    28921 tctaataa
//
LOCUS       DAOFMX010000054        28876 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.054, whole genome shotgun sequence.
ACCESSION   DAOFMX010000054 DAOFMX010000000
VERSION     DAOFMX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.054"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..654
                     /locus_tag="QBA20_002601"
                     /pseudo
     CDS             1..654
                     /locus_tag="QBA20_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            651..1037
                     /locus_tag="QBA20_002602"
     CDS             651..1037
                     /locus_tag="QBA20_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293091.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1062..3275
                     /gene="tssI"
                     /locus_tag="QBA20_002603"
     CDS             1062..3275
                     /gene="tssI"
                     /locus_tag="QBA20_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDQ6293092.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHEGRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDCIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3298..3743
                     /locus_tag="QBA20_002604"
                     /pseudo
     CDS             3298..3743
                     /locus_tag="QBA20_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3765..4172
                     /locus_tag="QBA20_002605"
     CDS             3765..4172
                     /locus_tag="QBA20_002605"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HDQ6293093.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4169..6646
                     /locus_tag="QBA20_002606"
     CDS             4169..6646
                     /locus_tag="QBA20_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDQ6293094.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWSQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDRSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELVKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDEQGLAVSRTDARGGEVALAHDARGQLRRYTDCSGCATGYE
                     YDEDGNLTAVTDAEGKTVRISYNRQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGS
                     VQSWQYTPRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQ
                     RPDGLIRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNR
                     LDQITEVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTD
                     ITLPDGQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADN
                     RLSWQRSLPGGSQNPQQAVLPRRRTTA"
     gene            6643..7632
                     /locus_tag="QBA20_002607"
     CDS             6643..7632
                     /locus_tag="QBA20_002607"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293095.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7732..7935
                     /locus_tag="QBA20_002608"
     CDS             7732..7935
                     /locus_tag="QBA20_002608"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293096.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            8037..8273
                     /locus_tag="QBA20_002609"
     CDS             8037..8273
                     /locus_tag="QBA20_002609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293097.1"
                     /translation="METHALSDDCLDCVYDWYAEKNGQFFSATSPEELLGLVAMWEVR
                     GNDWRANLNEINTYQKAIEESPFFDKNGNEIESD"
     gene            complement(8339..8571)
                     /locus_tag="QBA20_002610"
                     /pseudo
     CDS             complement(8339..8571)
                     /locus_tag="QBA20_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8681..9451)
                     /gene="yafV"
                     /locus_tag="QBA20_002611"
     CDS             complement(8681..9451)
                     /gene="yafV"
                     /locus_tag="QBA20_002611"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDQ6293098.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9632..10078
                     /gene="ivy"
                     /locus_tag="QBA20_002612"
     CDS             9632..10078
                     /gene="ivy"
                     /locus_tag="QBA20_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDQ6293099.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(10121..12565)
                     /gene="fadE"
                     /locus_tag="QBA20_002613"
     CDS             complement(10121..12565)
                     /gene="fadE"
                     /locus_tag="QBA20_002613"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDQ6293100.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12805..13383
                     /gene="lpcA"
                     /locus_tag="QBA20_002614"
     CDS             12805..13383
                     /gene="lpcA"
                     /locus_tag="QBA20_002614"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDQ6293101.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13589..14356
                     /locus_tag="QBA20_002615"
     CDS             13589..14356
                     /locus_tag="QBA20_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDQ6293102.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14327..15067)
                     /locus_tag="QBA20_002616"
     CDS             complement(14327..15067)
                     /locus_tag="QBA20_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDQ6293103.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15451..15972
                     /locus_tag="QBA20_002617"
                     /pseudo
     CDS             15451..15972
                     /locus_tag="QBA20_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            16148..16645
                     /gene="rayT"
                     /locus_tag="QBA20_002618"
     CDS             16148..16645
                     /gene="rayT"
                     /locus_tag="QBA20_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HDQ6293104.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16862..18574)
                     /locus_tag="QBA20_002619"
                     /pseudo
     CDS             complement(16862..18574)
                     /locus_tag="QBA20_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18546..19331
                     /gene="lafU"
                     /locus_tag="QBA20_002620"
     CDS             18546..19331
                     /gene="lafU"
                     /locus_tag="QBA20_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDQ6293105.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19402..20469
                     /gene="dinB"
                     /locus_tag="QBA20_002621"
     CDS             19402..20469
                     /gene="dinB"
                     /locus_tag="QBA20_002621"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDQ6293106.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRYTSRIE
                     PLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMN
                     KPNGQFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKR
                     FGKFGRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELER
                     RLAKVKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVR
                     LVGLHVTLLDPQMERQLVLGL"
     gene            20521..20814
                     /gene="yafN"
                     /locus_tag="QBA20_002622"
     CDS             20521..20814
                     /gene="yafN"
                     /locus_tag="QBA20_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HDQ6293107.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20817..21215
                     /gene="yafO"
                     /locus_tag="QBA20_002623"
     CDS             20817..21215
                     /gene="yafO"
                     /locus_tag="QBA20_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HDQ6293108.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21225..21677
                     /locus_tag="QBA20_002624"
     CDS             21225..21677
                     /locus_tag="QBA20_002624"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ6293109.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLTKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21923..22966
                     /locus_tag="QBA20_002625"
     CDS             21923..22966
                     /locus_tag="QBA20_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDQ6293110.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            23028..23642
                     /locus_tag="QBA20_002626"
                     /pseudo
     CDS             23028..23642
                     /locus_tag="QBA20_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23699..25156)
                     /gene="pepD"
                     /locus_tag="QBA20_002627"
     CDS             complement(23699..25156)
                     /gene="pepD"
                     /locus_tag="QBA20_002627"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDQ6293111.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25418..25876
                     /gene="gpt"
                     /locus_tag="QBA20_002628"
     CDS             25418..25876
                     /gene="gpt"
                     /locus_tag="QBA20_002628"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDQ6293112.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25968..27212
                     /gene="frsA"
                     /locus_tag="QBA20_002629"
     CDS             25968..27212
                     /gene="frsA"
                     /locus_tag="QBA20_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDQ6293113.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27270..27671
                     /gene="crl"
                     /locus_tag="QBA20_002630"
     CDS             27270..27671
                     /gene="crl"
                     /locus_tag="QBA20_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDQ6293114.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27710..28765)
                     /gene="phoE"
                     /locus_tag="QBA20_002631"
     CDS             complement(27710..28765)
                     /gene="phoE"
                     /locus_tag="QBA20_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDQ6293115.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7131 a         7184 c         7682 g         6879 t
ORIGIN      
        1 gaaaaaacaa atattgatgg caatatcatt atacataagg atggtaggag gacgtattta
       61 ttaaatcaaa atactggttc gattacggct gtgataagat ggaagtatga gtagtataag
      121 gataataccg ttacagatga ctggactata tgggaaaaag ctatttttat gcgagcgata
      181 tatttatctg ttcagcaggc atggaatggg aaaataactt attctgtttc cggtgaatct
      241 gagttcgcta aaaaatttca gggtaaagct ttgccttttg atgtaagaat aatttctgca
      301 tctcagaatg aagactggtt ggttattgca acaaaggttt tgcctggtgc tgatttaaga
      361 acatatgttg attttaaaaa cagtacggtt catgttgatt cagcgggcct ggaaaaagta
      421 gcgaaatgta ttaactgtaa taatacgttg caagtaaata tacctcatga agccggacat
      481 gtacttggtt acttagatga tgattatgat tcttcgtcac cgtatgttgg agatattagt
      541 gggttgatga atgtgggaat ggagcttcgg gaacgatacc ttaaaaacgc aactataacg
      601 ttgaatatca taatgcctga aactaaattt actttattga atgtaaccaa atgaaatttt
      661 tctgtattat agtgattttc ctttcaataa attacatagc tatagtgaat gttttcaagt
      721 cttctgcagt tgctcaatcc accaaatatt atgaagaaaa tgatatacat gatggaagta
      781 atgacatctt ttttatgaag aaatatgggc cgcctgctca tgagttcgtg aggccacgat
      841 ataaatttgt tggtgagtat tatatcggct tgaatcaaac ttatcgcata acagatcctg
      901 aatttcaaga tgttcctatt aaggaaatgt tctggaattt gcagggggat ttgaatctga
      961 catgttggtt tcattataaa gacgggcaat ggagagtgat tacttatatt ttttggccac
     1021 caggggctag attttaatcc ggtattaaag gagcaaatat catgagtttt gtatctacgg
     1081 gtaataaacc agttggaaac ggtggacccg tcatcaccac accaccaata gctggcgaaa
     1141 gtggcggtat gagcacaggt tcagctgtaa ccgatgtatc tggtgccgcc gaggagatgg
     1201 cagaacaggc tgcggcggac ctgtttggtg cgctgccgga accgtcaggt ctggttaaag
     1261 ccgcagtagc tgctgcgcag gctgccgctg cagcagcagg gatctcggat atggctggtg
     1321 cagttcagga tgcagcggcg tcgcttgcag ccggtgctcc gggggcgcat aacgtcacag
     1381 taagtggtag tgcggtgccg ccgcagatgc ttttgtttgc aggcatgaat ggttcggaag
     1441 ggcttgggaa tctgttttct tacacagtac agcttaaaac gctggacgcc ctgaacctgg
     1501 ggtacgtttc ccccgctgct aacctgccgc ttcagctcat ggtgggcaaa gacctgtgcg
     1561 tcagtatcga actggacggc ggcggaaaac ggtatatcag cgggctggtg acggcggcga
     1621 gggtggtcgg ccatgagggc cgttcggtaa cgtatgaatt acgcattgag ccatggctga
     1681 agctgctgac ccataccagc gactacaaag cgttccagaa caaaaccgtg gtggatattc
     1741 tcgatgaagt actggatgaa tattcctttc cggtggaaaa gcggctggtg gaaaattatc
     1801 caccccgcgc ctggcaggtg cagtatggtg aaaccgactt cgattgcatc cagcgcctga
     1861 tgcaggaatg ggggatttac tggtggtttg agcacagcga gaacagccac acgctggtgc
     1921 tggtggatgc catcaatggt cataaagcgt gccctgactc gccgctggtg gagtggcacc
     1981 aggaagggct gaaactggat aaggaattta tccacaccat tacggccagt gagaggctgc
     2041 gtactggaaa gtgggtgatg gacgattttg attttatgaa gcctcgttcg ctgcttaaaa
     2101 gtaccgtggc cagtccgcgt gataccggtc atgctgaata cgagcattac gaatggcctg
     2161 gtgattactt cactacgggc gaaggcgaga tgctgacgcg cattcgtatg gaagcgcagc
     2221 gcagcccagg cagccgggca catggggccg gtcatatccg taccctcatg accgggtaca
     2281 cctttacgtt gatgaatcac ccgacggcag aaattaatca ggaatatctg ctggtacaga
     2341 ccactctgtt tttacgggat aatgcgcagc acagcggtca ggatcagcat tttacctatg
     2401 tcaccacgtt tgaactgcac cccaccagcg aagtttaccg cccacaacga accctgagca
     2461 aaccccacac caaaggcccg cagagcgcca tcgtcaccgg cccggcgggg caggaaatct
     2521 ggacggataa atatggccgg gtgaaggtgc agtttggctg ggaccgttac ggaaagaatg
     2581 acgaaaacag ttcctgctgg atccgcgtca gctatccgtg ggcgggtaaa ggattcggca
     2641 tgatccagat cccgcgtatt ggccaggaag tactggtgga tttcaaaaac ggcgatccgg
     2701 atttgcccat catcgtgggg cgcacctata accaggacac catgccgccg tgggggctgc
     2761 cgggcatggc gtcgcagagt gggatattca gccactcatt gcagggaggg ccgactaacg
     2821 gaaatatgct gcgcttcgat gataaaacgg gtgcggaaga ggtgaagttc catgcggaaa
     2881 aagacctcaa taccacggta aaaaacaatg aaacccacac ggtcatggtc gatcgcataa
     2941 aaaccattgt caaaaatgaa accatcagcg tgggagaaga ccgtaatgcc accatcacaa
     3001 aaaatgatgg tctttccgtc aaactggctc aaaccatcaa tgtcggaacg acatatcgtc
     3061 tggatgttgg cgatcaattt acccttcgtt gcggcaatgc agcgcttgta ttacacaagg
     3121 atggctcgat tgagttttgc ggaaaacaac tgttactgca caccagcgat gttatgcaac
     3181 tcattggcag tggcattgat atgaatccgg atggcggcac agccatgact gcggatgata
     3241 ttgccccgtt acctaccccc gacgtttctg aataaaaaat ataaaaggaa tttttctatg
     3301 accagccttt accgtattca ggagggctgc tttgccctgc ctgaaacttt tcttgatcgc
     3361 accgtcaata tcttcgtccc atcgggcaat gaaagggcaa ctcccagtct gaatatttcc
     3421 cgcgatacgc tcaggccaga tgaaaatctg accacctata ttgaccgcca gatagccttg
     3481 atgaaaaaaa aaacctaggc cagcaccggg tactttcgcg gggaacggca ctggcgggaa
     3541 ctggcgaagc tgccatgaca ggtgaacaga ttctcgcaac tcatcgttct ggaaaaacag
     3601 aagtgtacca gcgtcaggcc gggtttatca ccgggccagc aaaggtacta atgttgacgc
     3661 tgaccactca gcgtcctttt gatgatcaca ccgaccagct atggactgaa tggttgacta
     3721 gttttcagcc tgctaaaagc taaaaaaaac agggaggttt tgtcatgtat gaagccgcac
     3781 gggtggatga ccccatctac cacaccagcg cccttgccgg gtttttgata ggggccatca
     3841 taggtatcgc cattattgcc gtcgccagct ttgctttttt tacctgcggc tttttggccg
     3901 ggctgatttt gagttttctg gctgatcaga tagcttccgg agtcctgcaa ctgggtgagg
     3961 ctattgggcg ctcgatacat tccacagtgg gaaaaattct gaccggttct gataatgtca
     4021 gcaccaacag tcgcccggcc gcccgggccg tactgagtac ggtgatatgc gaagaccatt
     4081 ccccggaaat ccgcattgcc caaggttccg gcaacatcta catcaacagc cagctgcccg
     4141 taaggatgac cacactgagt gtgatgcagt gattgaggac ggctccccca atgtctttct
     4201 cggcggcggt acgcagacgg tactggaaat cagcccggaa atccctgact ggctgcgtca
     4261 ggtggtggat gtgctgtatg tggtcgccgg aatgcttggc gggctggctg gcgcctggag
     4321 tcaggcggca aagatggggt cgaaatttgg tacaaaatgt gcggcaaaat ttattggtgg
     4381 cgaaatggtc gggatggcca tctctgacac agtcatggga ttgttcagca atccggtaga
     4441 tgtcaccact gggcaaaaaa ttttgctgcc ggagacggat tttaccctgc caggacgcct
     4501 gcctgtcacc tgttcgcgct tttacgccag ccacctggaa actgaaggat tgctgggcag
     4561 aggatggcgg ctgaactggg agataaacct gcgtgaggat gaaacatata tcaccttcat
     4621 cggtgtacag ggtcgggaac tgagttatcc gaaagagatg ctgataccgg gccaccagat
     4681 attcgacccg gaagagcagt tttacctgag ccgtctgcat gacggatgct atgtgctgca
     4741 ttacaccgac cgcagctatt acgtgtttga tgaatttgat gaccacggcg tcgcgccgtt
     4801 actttttatg gagacgccgt accggcagcg tattgctttc gggcgtgaga acggcaggct
     4861 ggtgcgggtg gcctccagca gcggacacca tctgttattg caccgcacga tgacgcaagc
     4921 aggggaacgc ctgtcgcata ttgaacttgt gaagggcggg cgtcccggta acctggtgga
     4981 atatcgttac gacgataacg ggcaactgac tggcgtggtg aaccgggctg gtgtgacggt
     5041 gcgccagttt gcctatgaaa atggcctgat gacagagcat cgcaacgcga cgggctttac
     5101 ctgtacctac cactgggaag agattgaagg tttcccgcgc gtggtggaac acacgaccag
     5161 cgacggcgag gattaccggt tccactatga ttttgctggc gggcagacgg tggtaaccgg
     5221 caggccggag cagaagtggc agtggtggtt tgatgaagag acgtatgtga ctgcgcaccg
     5281 gacgccgggc ggcggaatgt accgcttcac gtacaacgaa aaccattttc cggtagcggt
     5341 ggaactcccc ggcgaacgca gggtaacgct ggaatacgac accctgagcc gggtggtgaa
     5401 agagacggac ccggcaggcc gggtgacaca gacgcagtgg aacggcagtt ttgctgaaat
     5461 cacgcgccgg gcgctggatg acgaccacgt atggaaggcg gattacaacg aacacggcca
     5521 ggtgatacgg gagacggacc cggaagggcg cgtcacgcgg tacgggtatg atgaacaggg
     5581 gctggcggtc agccgcacgg atgcgcgggg cggcgaggtt gcgctggcgc acgatgcgcg
     5641 cggtcaactg aggcgttaca ccgactgttc agggtgcgcc acgggttatg aatacgatga
     5701 agacggcaac ctgacggcgg tgacggacgc ggaggggaaa acggtacgca tcagctataa
     5761 ccgtcagggg ctgccggaga cggtctgcca ccccggtaaa cagcaggacc gttacacctg
     5821 gaacgcgctg gggttactga gcagccaccg tcgcatcacg ggcagtgtgc agtcctggca
     5881 gtatacgccg cgcgggatgc tggcgcgcca tacggatgaa gagaaacggg agacgcgctg
     5941 gcagtacacc ccggaagggc tggttgccag tctgagcaac ggcaacgggg ctcagtaccg
     6001 gttcagctac gacggcgacg gacggctgac gggagaacag cgcccggacg gcctgataag
     6061 gatgtttgcg ctgaatgcgg acgggtttcc ggtcatcatc ccgacgcagg gcacagaggg
     6121 cggggtgcgc aatgaacagc aggagcgtga tgcgctgggc cgcctgctgc gcagtgacac
     6181 gcagcacagt acacggacct tcagctacaa ccgtcttgac cagataacgg aagtgacgct
     6241 cacccccacg gaagaagggg aacggctgca ccacatgcag gcggacacgg tgcgttttgc
     6301 gtatgaccgc agcgggtggc tgacggcaga gcacagcgtg cacggcagta tcaaataccg
     6361 gcgcgatgcg ctgggcaatc ccaccgatat cacgttgccg gacgggcagc atctcagtca
     6421 tctttactac ggcagcgggc atctgctcca gacggcactg gacggtatca cggtcagtga
     6481 atatgagcgt gacagcctgc accgtcaggt gatacgcacg caggggaaac tggcgacgtt
     6541 cagcggctac aacgccgaca accggttaag ctggcagcgc agcctgccag gtggcagcca
     6601 gaatccacag caggcggtac tcccccggcg cagaacgaca gcgtgacggc gcgggactat
     6661 atctggaacg cagacggtga agtcggcggc atcaatgaca aactgcgcgg ttgcctggtg
     6721 ttcagctatg accgcagcgg ctggctgacc agccgcacgg ggcagatgta tgaccatgac
     6781 cattattatt acgacaaagc gggcaacctg ctgacggatg agtatcaggg agcggtgatg
     6841 gataaccgcc tgccgggtta cgggcgtgac cgctaccgct ataacgaatg gggcgaactg
     6901 acagagcgca gggaccagca gctggagtgg aacgcgcagg gccagttaac gcgggtcatc
     6961 agcagcaaca gcgagacgcg gtatcagtac gatgcgctgg gcaggcgcat cagcaaggcg
     7021 accagcaatc tccataccga ccggggagaa cgaagccgga caacgtttgt gtgggaaggg
     7081 ttccggctgt tacaggagac aacgtggcag ggaaagcgga catacctgta cgatgcggaa
     7141 cagccgtaca cgccggtggc tgccatcacg gggcgcggag agagccagaa aatctggtat
     7201 taccacacgg acctgacggg cacggtacac gaggtaaccg cgccggacgg gacgctggtg
     7261 tgggcgggct accaggcggg atttggtgaa aaccggggcg acatcagcaa cagcggggcg
     7321 tactttgagc agccgcagcg gctgccgggg cagtactttg acgaagaaac agggctgcat
     7381 tacaatctgt tcaggtatta tgcgccggag tgcgggaggt tcgtaagcca ggacccgata
     7441 ggattaaacg gtgggttgaa cttatatgct tatgcgccta atccgttagg ttggatcgat
     7501 ccgctaggac tgaattcttt aggaatagat tcaaataata tatttagagg agatggtaac
     7561 tataaaggtg gcggaattgg acagccaaag agtggcacaa tatcagcaca ggacattatt
     7621 gatcatctct aagaaaaagg tgatgggcgt ttaacatctt ttagtactaa aaaaacatct
     7681 agttccaact ctcgtggtgc agattttttc tcaaaaaacc atgtcttcaa agtggcagtt
     7741 tctgatctcg atgatttagt taaaaataat cagatcactg ttttaacaca agaaaaagta
     7801 aaagcaatat ttggtttaga atcaagcaag aaaattagaa aagaacttgg taatattctt
     7861 cagaatatgg ctaggaataa tgaggtgtta gttcatgggc aaattcttgg aaataaagtt
     7921 agccgatgta attagttagt ggagaggaag ttgtggacga aattagaata agaattgctg
     7981 gtaatacggc tgcgccttgt ttccatgtgc taaaaaataa gggataaaaa gtaacaatgg
     8041 aaactcatgc attatctgac gattgtttag attgtgtata tgattggtat gcggaaaaaa
     8101 atggacaatt ttttagtgca acgagtccag aagagttact tggattagtt gcaatgtggg
     8161 aagttcgtgg taatgactgg agagctaatt tgaatgagat aaatacttat caaaaagcca
     8221 tagaagaatc cccgtttttt gataaaaacg gcaatgaaat agagtctgac taaattttat
     8281 tgatatagca acgataataa ccggaaattt cttccagatc tttccggctt tttattattt
     8341 aactttcgtt cagcacacta aacatcccgt ctcgcatccc cttaactggc tgtttaacct
     8401 gataaagttc cctgcgcagt tcctgtactt cgttgttatc ccaagcagga tcaaacatcc
     8461 ctccgagatc ttcacggtaa cgcccgtgcc gatctcaaat cccgcttttc tcagccattt
     8521 ggcgctgatt ttgatcaccg gcgtgtcgct gcggttgggc agataaccca cggtacagcg
     8581 acgctgtgtt ttggaaatgg tgacttctgg cgtaatattg tgtttaagtt aggggctttc
     8641 gtaatctagc ccagcccccg gccctaaaca caacccccac tcaccacaac ctaaactcat
     8701 ccgcatcccg ccatgccgga aacttttctc tatattcccg caatgccacc atcgacagct
     8761 ccgcatcaat acgcgttgcc ttatgcgcgt cggcggtagc gataatctcg ccttgcggat
     8821 taatcacccg gctgtcaccg cgataatggc agccgttgcc atcgctgccg acgcgattgc
     8881 atcccgccac atacgcctga ttctcaatcg cgcgggccgt cagcaatgcc tgccagtgca
     8941 gagagcgcgg agctggccag ttggcgacgt acagggcgag gtcataatcg ttgagattgc
     9001 gcgaccacac tggaaaacgt aagtcgtagc acaccagcgg caaaatacgc cagccgcgcc
     9061 attccacaat cactcgcgca ttgcccgctt tataatgcag atgctcatct gccatgcgga
     9121 acagatgacg cttatcataa aaatgtaccg tgccgcccgg ctcaaccagc aaaaagcggt
     9181 taaccgaacc agactccgtt tgtaatgcaa cactgcctgc aatcagcgca ttgcactgct
     9241 gcgccttagc tgtcatccag ttcactacgt catcttgtgc aagcgacgaa gctgccgctt
     9301 ccatggcaaa gccgctggta aacatctccg gtagaacgat cacatcgcgc ccggtaatac
     9361 cttccagttg acgatcaaaa tggcgcaggt tggcaggacc atccacccac accaacggtt
     9421 gctgcaaaag cgtaatcttc aaactaggca cggtgtacaa ctcctttatg cgaagggttt
     9481 tataacttta acaccttatc aggcagttgc cttagcgcag aataaattga taacaaatgc
     9541 tgatattgga aatatctgat ttgcaaatta tcgtgttatc gctaggcttt aggaggttaa
     9601 taacatgggc aggataagct cgggaggaat gatgtttaag gcaataacga cagtcgccgc
     9661 actggtcatc gccaccagtg caatggcgca ggatgattta accattagca gccttgcaaa
     9721 gggcgaaacc accaaagctg cgtttaatca gatggtgcaa gggcataagc tacctgcatg
     9781 ggtgatgaaa ggcggtactt atactcccgc acaaaccgta acgttgggag atgagacgta
     9841 tcaggtgatg agcgcgtgca aaccgcatga ctgtggctcg caacgtatcg ctgtgatgtg
     9901 gtccgagaaa tctaatcaga tgacggggct gttctcggct attgatgaga aaacgtcgca
     9961 agagaaactc acctggctga atgtgaacga tgcgctttcg attgatggta aaacggtgct
    10021 gttcgcggcg ttgaccggca gcctggaaaa ccatccggat ggctttaatt ttaaataatt
    10081 agcggataaa gaaacggagc ctttcggctc cgttattcaa ttacgcggct tcaactttcc
    10141 gcactttctc cggcaacttt accggcttcg tcgccagctc ttccggatca aagtcatcaa
    10201 cgttaatact gcgcagacgg ctttcttccg ctttcaccag aatagcggct tcatctttat
    10261 caatcagccc cttcgtcagc gcgttgtgcg ccagttcatc cagacgggta aatggcagat
    10321 ttttacccag ctctttacag atccgctgat gaatcgggtc ggcggcaatc acatccacca
    10381 acgcctcttc cagtaaacca accgggttat gctcgcttgg cgtcaggtac tgaccgcgac
    10441 caatacgtga acgagtggcg ttcggcactt gtaaaatctt cgccacttta tgatccagct
    10501 tgtcagacgg tgccagataa tgacgtccgg tcgggaagat caccacattc agcagcccgg
    10561 caaccacgcg gttcgggaag ttttgcagta aattgtccat tgcctgttct gcctgataca
    10621 gcgcatcctg tacgccccag tgcaccagcg gcagatcggc ttcattacgg ccttcgtcgt
    10681 cataacgctt cagaacggca gaggcgaggt aaagctggct taaaatatcc cccagacgtg
    10741 ccgagatacg ctcacgacgt ttcaggctgc cgcccagcag tgccatcgag acatcagaaa
    10801 gcagggcgag gttggcgctc aggcggttca ggtgctgata gtagcgttta gtggcatcgc
    10861 cggttggcgt gctgctggtt aaaccgcgcg tcaggcccag ccagaagctg cgaactttgt
    10921 tgctaccgac gtgaccgata tgtttgaaca acagtttatc gaacgcgttg acgtcattgt
    10981 tcttcgccgc ttccatctct tccagcacgt acggatggca acgaatcgct ccttgtccga
    11041 agatcatcat gctgcgggtc agaatgttag ccccttcaac ggtgatggca atcggtgcgc
    11101 cctggtaagc acgcgccagg aagttgcttt gcccgagcat aatgccttta ccgccggtaa
    11161 tatccatcgc atcaataatc gactgctgcc cgcggtgggt acagtgatac ttaacgatag
    11221 ccgacagcac ggcaggtttt tcgccgagca taatgccgta ggtaatcagc gatgccgcag
    11281 catccattac ataagcgttc ccggcaatac gcgccagcgg ctcttcaatc ccttccatct
    11341 taccgataga gattttgaac tgacggcgaa tgtgagaata cgcgcccgtt gccagcgcta
    11401 ccgatttcac gccgccggtt gagttggaag gcagggtgat gccgcggcct accgagaggc
    11461 actccaccag catccgccag ccttgcccgg ccattttcgg cccgccgatg atgtaatcga
    11521 tcggcacgaa gacatcttta ccgcgcgtcg gtccgttctg gaacggtacg ttcagcggga
    11581 agtggcgacg accaatttcc acgcccggcg tggtggttgg gatcagcgca caggtaatgc
    11641 ctaaatcttc tgcaccgccg agtaattttt ccgggtcgga gagtttaaac gccagcccaa
    11701 gcacggtcgc aatcggtgcc agcgtaatgt agcgtttgtt ccaggtcaga cgcatcccca
    11761 gcacctgctg gccctgccat tcgcccatgc agacaatccc ggtgtccgga atcgcgcccg
    11821 catcggaacc cgcttccggg ctggtcagtg caaagcaggg gatctcctga ccacgcgcca
    11881 gacgcggcag atagtgatct ttctgctcgt cagtgccgta atgttgcaac agttcgcccg
    11941 ggcctaatga gtttggcacg ccgacggtaa tcgccaggat cccgctcacg ccagagagtt
    12001 tttgcagcac acgagactgg gcataagctg agaactccag tccgccgtac tcttttttga
    12061 taatcatcgc gaagaagcga tgctctttaa ggtacgccca caactccggc ggcagatccg
    12121 ccagctcatg agtaatctgg aaatcattcg ccatccggca ggcttcttct accgggccgt
    12181 cgagaaacgc ttgctcttca gcggtcaggc gcggctgcgg atagttatgc agctttttcc
    12241 agtccggctt gccctggaac aagtcgccct cccaccaggt ggtgcccgca tcaatcgctt
    12301 ctttctcagt gcgcgacatc ggcggcatca ccttacggaa accgcgaaat accggcgcgg
    12361 aaatcatcga cttacgcata ggcgcaaagt taaatggcac gaggataatg gtcagaggca
    12421 ccagtaccca cgccgaccac agaccagcaa cgccgagggc ggctgtccag gcgagcaaaa
    12481 tcagactgct gataaataag ctcacgcggt gatagaacaa cgcgccgagc aggacaaccg
    12541 tagcgagaat actcaaaatc atcataacga aaagcccctt acttgtagga ggtctgacca
    12601 cttgtgatga tatggttgta gtggatgtaa aaacatttag caatgtgttt acaatataat
    12661 tacaacaaag ctcacattgt tgctgttttt atccgcactt caggtcaaaa agtcctggtc
    12721 atagcacctg cccgtacttc tcgcttttgg cggtatccgg tacactgcat tttgtctatt
    12781 acatttatgc tgaaggatat cctcatgtac caggatctta ttcgtaacga actgaacgaa
    12841 gcggcggaaa cgctggctaa ctttttaaaa gatgacgcca atattcacgc cattcagcgc
    12901 gcggcggtcc tgttagcaga cagctttaaa gccggtggca aagtgctttc ctgcggcaac
    12961 ggcggttccc attgcgacgc tatgcacttt gccgaagagt tgaccggtcg ctaccgtgaa
    13021 aaccgtccgg gctacccggc gattgctatt tctgacgtta gtcatatttc ctgcgtcggt
    13081 aatgatttcg gtttcaatga tattttctcc cgctacgttg aagcggtagg tcgcgaaggc
    13141 gatgtactgc tggggatctc cacctccggt aactctgcaa acgtgatcaa agcgatcgca
    13201 gcggcgcgtg agaaggggat gaaagtgatc accctgaccg gtaaagacgg cggtaaaatg
    13261 gctggcacgg cggatatcga aattcgcgtt ccgcactttg gttatgccga ccgcattcag
    13321 gagattcaca ttaaagtgat ccatatcctg atccagttaa ttgaaaaaga gatggttaag
    13381 taagtctggc gtaggccgga taaggcgttt acgccgcatc cggcacttgt gcgctgatgc
    13441 ctgatgcgat gctgacgcgt cttatcatgc ctacaaatct gtacccgaac cgtaggccga
    13501 ataattcgtt tacgccgcat ccgacctgaa aattcttaaa tcaatcttcg ccggggtcca
    13561 tgcgctcccg ctgttgtgga ggttacccat gtgcgaattg ctcgggatga gcgccaacgt
    13621 ccctaccgat atctgcttta gtttcaccgg acttgtacag cgtggtggtg gaaccgggcc
    13681 acataaagat ggctggggca ttacctttta cgaaggtaaa ggctgtcgca catttaaaga
    13741 tccacaaccc agctttaatt cccccatcgc caaacttgtc caggactacc cgataaaatc
    13801 ctgttcggtg gtggctcata ttcgccaggc taatcggggc gaggtggcgc tggaaaatac
    13861 tcacccattt acccgcgagt tatgggggcg taactggact tatgcccata acggacaact
    13921 gacgggctac aaatcactgg aaaccggcaa cttccgcccg gtcggtgaaa ccgacagcga
    13981 aaaagccttt tgctggctgc tgcataaatt aacgcagcgt tacccgcgca cgccgggcaa
    14041 catggcggca gtgtttaaat atatcgcctc actggcggat gaactgcggg cgaagggcgt
    14101 tttcaacatg ctgctttcgg acgggcgcta tgtaatggcg tattgctcga ctaatttaca
    14161 ctggatcacc cgccgcgcgc cgtttggcgt ggcaacgttg ctggatcagg atgtggaaat
    14221 cgacttcagc tcgcagacca caccgaatga tgtggtcacg gtgattgcga cacagccgct
    14281 gacgggcaat gaaacctggc aaaaaattat gccaggcgaa tggcgcttat tttgcctcgg
    14341 ggagcgtgta gtttgatgcc agttgtggct gcaccacttc gtggcttaac ggcttgctga
    14401 ccacgtaacg gccattggcc acagaaacgg tcggtggctt acgggtttgc tcaaagtagt
    14461 cgtagcccgg cttcagctgc tcccagaagt ccttaaagtt ggaatattta tggcgcttca
    14521 tattggcgtc ggtcatgcgg aatgggtaaa tactcacctg cacgctcggc tgaccaaaca
    14581 ccagcgcacc agtaacgaac tggaagatct catcaatacc ctgattggtc attgcgtagc
    14641 agccgatgga tacacaatcg ccgtggatca tcaggtattt cccttcataa ccatgcgcac
    14701 ggtcataagc attggggaat ccaatattaa tcgctttgta gtaacggctg tctggtttta
    14761 actgattacg ctggacgcta taaaaccctt ccgggctttt gaaatcgccc tgacgctgtt
    14821 ttggccctaa gccgccggaa tatttacaga ttttataact gtcgagcagt tgatattgct
    14881 cgcccatttt gacgtaaaga tcgagcgtac gttcttcctt gaagatctgg atgtagacag
    14941 gggatcccat caactgctgc ttatactctt tgctcactgg cgtggtcgag ctactgctgc
    15001 ccagcaaacc ggcaaacgaa acgcacggga tcaacaacat cgcaagaatt aatgcgattt
    15061 tacgcatact gcttattcct tgataaaacg gttacacacg ccaggacggc aaaatggatc
    15121 ccaaatcgga atagtctgga tttggaaggc tcacattatc accaaaagag ttttacgcaa
    15181 gcctgtcggc gctgggttta caaatttagc acaggggcag ttttcaatag tccgaaaagc
    15241 gcatgtatca tttatttcct aatattatga aatctcaata gtaatatgca aataagcaat
    15301 taataatccc gtttactcct ccggtctttt aaaattctca ccttcattca gccggtttat
    15361 gtctttcatg tcttcgttct tgctcggcag atttttgcat ccaggcgtgt tttcgttatg
    15421 cgttttgctc cctttactcg ccagcgccac cacaaccggg agcagataaa acagcatcca
    15481 gaatggtttc ctgcgccgct caaggcgagt gacagacgct ggcaggcgct ggcggaaaac
    15541 aaccactttt taagcagcga tcacctgcat aacatcaccg aagtggcgat tcaccgcctg
    15601 gagcagcagc ttggcaagcc ttacgtctgg ggcggcacgc ggcccgatca aggcttcgat
    15661 tgtagtgggt tagtttttta tgcctacaac aagatccttg aggcgaaact cccgcgcacg
    15721 gccaatgaga tgtaccacta ccgtcgggca acgattgtgg cgaataacga cctgcgccgg
    15781 ggagatttgc tatttttcca tatccacagc cgcgagattg ccgaccatat gggggtgtat
    15841 ctgggcgacg gtcagtttat tgagtcactg cgcaccgggg aaaccattcg ggtaagccgg
    15901 ttagccgaac ctttctggca ggaccatttt ttaggcgcgc gcaggatttt gacggaagag
    15961 atgattttgt aggccggata aggcgtttac gccgcatccg gcggttgtgc acggatgcct
    16021 gatgcgacgc tgacgcgtct tatcatgcct acaaatctgt gcgtttttgc gagccgcttt
    16081 cccgatataa cacagcgtca ttgtcggcct ttccttttcc tcttaccgtt gagaaaaagg
    16141 agtcgctatg tcggaatatc gtcgttatta catcaagggg ggaacatggt ttttcacggt
    16201 aaatttacgc aatcgtcgaa gccatcttct gaccacccag ttccagacgc tccgtaacgc
    16261 cattattaac gttaagcgag acagaccttt tgaaattaac gcctgggtcg ttttgccgga
    16321 gcatatgcat tgcatatgga cattacccga aagcgatgat gatttttcct ctcgctggcg
    16381 ggagattaaa aagcagttca cccatgcttg cggattgaaa aatatctggc aaccgcgttt
    16441 ttgggagcat gccattcgca atacaaaaga ttatcgccat catgttgatt atatttatat
    16501 aaatccagag aaacatggct gggtaaagca ggtgagtgat tggccattct caacgttcca
    16561 tcgcgatgtc gcgcgagggt tatatcccat cgattgggcg ggagacataa cggatttaag
    16621 tgccggggag cgtatcattt tataactgtg cgctggtgcc ggatgcgctt acgcttatcc
    16681 ggcctacaaa tcgtgcttta acggtaggcc ggataaggcg ttcacgccgc atccggcagt
    16741 tatgcgcaga tgcctgatgc gacgctggcg cgtcttatca tgcctaaaaa cttgtgcctg
    16801 aacggtaggc cggataaggc gtttacgccg catccggcaa ttgtgcaccg atgccggatt
    16861 cttacatcaa cgcccccata atcttcaact ccagctcatc cggcacttcg ttatacgaca
    16921 gcacatgcag ccccggcgca aacaaccttg cataacgcgc cagcaaaggg cgtagctgcg
    16981 gcggcaccag cagcaccgga tctttccccg ccgctttcat ctgctctttc acctgtggca
    17041 tcgtgctctg gaactggttg agcatattcg ggtcaaccgg cacgctgtcg agcatcactt
    17101 tgccggcctg ttgcgcctga ttcactacgt tggtcagcag attttccagc tcattattca
    17161 gcgtatacac cgtcagctcc tgcttgcgaa cgaacggatg agtaatgctg cgccgcagcg
    17221 ccaggcgcac atcggccgcc agcagaatat gatctttagt caccgtgcta ctggcgacca
    17281 gcacggtggc gatggtgacg atatcgcgca gggaaacgcc ttcggtcagc agcgcccggt
    17341 acactttcag caattggctg taattgagcg ccgcgcttaa atcctccgcc aggcgcggtg
    17401 ccatcgacga caaacggtta tgcaactgcg tgatgtcatc atagttaaac aaatcaggaa
    17461 tatagctgcg cacaatcttg ttcacatggg tggcaatcac actggcgctg tcgatcacct
    17521 gataccccat attcagagcc ttcgctttct gcgcaggctg gatccaggtg accggtatcc
    17581 cgtacgccgg gtcgttcccc agcacgccgt caatctcgcc gtaggtttcg ctggagggca
    17641 gcgccatcag tttatccgcc ggaatatccg cttcatcagc ctcaatgccg ttgatgaaaa
    17701 tagcgtactg gctgggcttg aggcggaagt tttcccgaat gcggatctcc ggcagcagca
    17761 cgccgttacc gtcggaaatc acctgccgaa cgccgcgaat ccgctgggtg agcgggttgc
    17821 cctgcgcttt gtccaccagc gccaccagct tgtagccgag gcttaagctg atgggctcga
    17881 tcagcggaat agtttcccaa ctgacctgtt gttcgctggt ttcagtgatg gtgcgggtca
    17941 gcgtttcgag gcttttctct tccgcctccg ccgcctgcgg gcgtttgctc atccgccagc
    18001 cggtaaagcc aagcagggcg ctgaacagca aaaacggcag atgcggcatc cccggcacca
    18061 ccgccagcac gaacatgatc ccggtggcgg tgtagagcac cgacgggctt gccagcaact
    18121 ggtggcgcac gtcatgggcg atatcgccgt tgtcgctgac gcgggtgacg ataatcgccg
    18181 ccgcggtgga gagcagcagg gaagggatct gcgccaccag gccgtcgccg atggtcatca
    18241 gcacgtattg ctggaaggcg gcgtcggcgc tcaggttgta tttgaagatg ccgatacaga
    18301 cgccgccgct caggttgatc gccagaatca tcatcccggc gatggcgtct ccgcgtacaa
    18361 acttcgatgc cccgtccatc gcgccgtaga aatcggcctc gctggcaaca tctttacgcc
    18421 gggtttgcgc ctgcgcctgg ttgatcaatc cggcgttaag atcggcgtca atcgccatct
    18481 gtttgccggg catcgcgtcc agggtaaagc gggcagaaac ctcggaaata cgctcggcct
    18541 ctttggtgac gacgataaag ttgattgtca acagcgtcag taaatccgag cgtgaaagca
    18601 ttatcgccgc gctgcacggg cagtcgattt ttaacggtgg tgggctgtcg ccgttaaata
    18661 aaatcagccc gtctcatccg ccaaaacctg ccaccgtggc ggtgccggaa gagacggaga
    18721 aaaaagcgcg tgatgtgaat gagaaaacgg cgctgctgaa gaagaaatcc gccaccgagc
    18781 ttggcgagtt ggcaaccagt atcaacacca ttgcccgtga tgcgcatatg gaagcgaatc
    18841 tggagatgga gattgttccc cagggattac gcgtgctgat taaagacgac cagaatcgca
    18901 atatgttcga acgcggcagc gcgcagatta tgccgttctt taaaactttg ctggtggagc
    18961 tcgcgccagt gttcgactcg ctctataaca aaattattat taccgggcat accgatgcga
    19021 tggcctacaa aaacaatatc tacaacaact ggaacctttc gggcgatcgc gcgctttcag
    19081 ctcgtcgggt gctggaagag gccggaatgc cggaaaataa agtgatgcag gtaagcgcaa
    19141 tggcggacca gatgctgctg gacgcgaaga atccgcaaag cgcgggcaat cggcgtattg
    19201 agattatggt gctgaccaaa agtgcgtccg atacgctgta tcaatacttt ggtcagcatg
    19261 gggataaagt ggtgcagccg ctggtgcaaa agctggataa gcagcaggtg ctttcgcagc
    19321 gaatgcgcta aatgctgaat ctttacgcat ttctcaaaac ctgaaatcac tgtatacttt
    19381 accagtgttg agaggtgagc aatgcgtaaa atcattcatg tggatatgga ctgctttttc
    19441 gcagcggtgg agatgcgcga caatcccgct ctgcgcgata tccctattgc tattggcggc
    19501 agccgcgaac gtcggggggt gatcagcacc gccaattatc ccgcgcgtaa atttggcgta
    19561 cgtagcgcta tgcctactgg gatggcgctc aaattatgcc cacatctcac cttgcttccg
    19621 gggcgctttg acgcctacaa agaagcctca aatcatatcc gtgaaatctt ctcgcgctac
    19681 acctcgcgct acacctcgcg cattgaaccg ttgtcactgg atgaggctta tctcgatgtc
    19741 accgatagcg tccattgcca cggttctgcg accctcatcg cccaggaaat ccgccagacg
    19801 atcttcaacg agctgcaact gacggcatct gcgggcgtgg caccagtaaa gtttctcgcc
    19861 aaaatcgcct ccgacatgaa taaacccaac ggtcagtttg tgattacgcc ggcagaagtt
    19921 ccggcatttt tacaaacctt accactggaa aaaatccccg gcgtcggcaa agtctcggcg
    19981 gcaaaactgg aagcgatggg gctgcggacc tgcggtgatg tacaaaagtg tgatctggtg
    20041 attctgctta aacgctttgg caaatttggc cgcattttgt gggagcgtag tcaggggatt
    20101 gacgagcgcg acgttaacag cgaacggtta cgaaaatccg tcggcgtgga acgcacgatg
    20161 gcggaagata ttcatcactg gtctgaatgt gaagcgatta tcgagcggct gtatccggaa
    20221 cttgaacgcc gtctggcaaa ggtaaaacct gatttactga ttgctcgcca gggggtgaaa
    20281 ttaaagttcg acgattttca gcagaccacc caggagcacg tctggccgcg gctgaataaa
    20341 gctgacttaa tcgccaccgc gcgtaaaacc tgggatgaac gccgcggcgg gcgcggtgtg
    20401 cgtctggtgg ggctgcatgt gacgttgctt gacccgcaaa tggaaagaca actggtgctg
    20461 ggattatgat gtatactatt atgtatattc tggtgtgcat tattatgagg gtatcactgt
    20521 atgcatcgaa ttctcgctga aaaatcggtc aatatcactg agttacgtaa aaacccagct
    20581 aaatacttta ttgatcaacc agttgcggtt ctttctaata atcgccccgc aggatatctc
    20641 ttaagtgcca gcgcattcga agcgttaatg gacatgcttg ctgaacaaga ggagaaaaag
    20701 cccataaagg cgcgctttcg tccaagtgct gcaagattag aggaaattac acgccgcgct
    20761 aaacaatatc ttaatgatat gacggatgat gatttcaatg actttaagga ataaggatgc
    20821 gggtattcaa aacaaaactt attcgcctgc aacttacagc agaggaactt gatgcgttaa
    20881 cggcggattt tatttcctat aagcgtgacg gtgttttgcc agatatattt ggtcgcgatg
    20941 cactctacga cgactcgttt acctggccat taatcaaatt tgagcgagtt gctcatattc
    21001 atctggcaaa tgtgaataat ccatttccgc cacagttgcg ccaattcagc agaacgaatg
    21061 acgaagcgca tttggtatat tgtcagggtg cgtttgatga gcaagcatgg ttgctcattg
    21121 ccattctgaa gcctgaacct cataaactgg ctcgagataa caaccaaatg cataaaattg
    21181 gaaaaatggc agaagcgttt cgcatgcgtt tttgaattta tattatgaat aacatacaaa
    21241 taagagatta tcagcctggc gattttcagc aactatgcgc tattttcatt agagcggtta
    21301 cgatgaccgt cagtcagcat tattcactac aacaaattgc cgcctgggcg cagattgacg
    21361 aatctcgctg gaaggagaaa ctcacgaaat cacaagtgcg ggttgcagtt attaatgcaa
    21421 aactggtcgg ctttattacc tgcgttgaac actatatcga tatgttattt gttgaccctg
    21481 aatacacccg ccgtggggtt gccagcgctt tgttaaaacc ttttattaag tctgaatccg
    21541 aacttacggt ggacgcaagc ataaccgcaa aacccttttt tgaacgttat ggttttcaga
    21601 cagttaagca gcagcgcgtt gaatgccggg gagcgtggtt tattaatttt tatatgcgtt
    21661 ataaaccgca acattaaatc cagcttgcaa tgaaaataac gcccgcctag tatgtgcgcg
    21721 ttttcttatt ccacaaactg caaggaggta aatcatgaca aatcctttat caatgactct
    21781 ttgcagacct ttccaggatt aattcttttt ttcttgccct ggattcgtct gccatttcct
    21841 gattttttaa ttgttactct caataattca agtcgcagca catgcaactt gaagtatgac
    21901 gagtctattt acggaatggt atatgggcaa ttatattcgt cccttatccg atgcggtatt
    21961 taccatcgca tctgatgacc tgtggatcga gagtttagcg atccaacaat tacacaccac
    22021 ggcaaattta cccaacatgc agcgcgtagt ggggatgcca gatttacacc ccggacgcgg
    22081 ctacccgatt ggcgcagcgt tcttctccgc aggccgtttt tacccagcac tggtcggcaa
    22141 tgatatcggc tgtggcatgg cgctatggca aacagatatt ctcgctcgca aatacaacgc
    22201 cgataagttt gaaaagcgat tatcagcttt ggatgacgtt gctgaagaaa gctggctgga
    22261 ggaaaacctg ccgtcagcat ttgcacagca tccgtggcgc agctcgcttg gctctatcgg
    22321 tggtggcaat catttcgctg aactgcaaca agttgatcaa atcatcaacg ctgaactgtt
    22381 tgcactggca ggtctggatg cgcagcatct gcaactgctg gttcatagcg gctcgagggg
    22441 gctagggcag tctattttac agcggcatat tgcctcgttt tcgcatcatg gtttgcctga
    22501 aggcagtgac gacgcgctgg cgtttgcgcg ttttaatcgc cacctgatcg ctttgcgcat
    22561 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
    22621 cgcgtgtcga atcggtgatc aacaaggggt cttacatcgt aaaggtgcca cgcctgatga
    22681 ctgtggtctg gtggttattc ccggttcacg cggtgattac tccaggctgg ttcagcccgt
    22741 caggagtgag gaaacattgc attcgctggc gcatggggct gggcgtaaat gggggcgcac
    22801 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg caactctcgc gtactgaact
    22861 tggcagtcgg gtaatttgtc gcgataaaca actcatcttc gaagaagcgc cacaagccta
    22921 taaatcggct gaaagtgtgg tgcaatgtct gggcaggccg gattaattat tcctgtcgcg
    22981 cggctgcgtc cggtgctaac gctcaaaaac agtggaggga aaaaaggatg atcttgctat
    23041 aactctcctc agcgcagggg ccggaagaat gttgtctcgc agtgaaaaag gcgctggaca
    23101 ggctaattaa agaagctgcc cgacaagacg tcgcggtaac ggtgctggaa acagaaacag
    23161 gccgctactc tgacacgctg cgttcggcgc tgatttctct ggatggcgac aacgcatggg
    23221 cgttaagcga aagctggtgt ggcactattc agtggatttg cccgagcccg tatcgcccac
    23281 atcacgggcg taaaaactgg tttctgggca ttgggcgttt taccgctgat gagcaggagc
    23341 aatccgatgc gatccggtat gagacgctgc gttcgtcggg gccgggcggt caacatgtca
    23401 ataaaaccga ctcggcggta cgcgccacgc atttggcatc cggtattagc gtgaaggttc
    23461 agtcagagcg cagccagcat gctaacaaac ggctggcgcg attgctgatt gcctggaagc
    23521 tggatcagca gcaataggaa aatagcgcgg tgctgaaatc acagcggcga atgttccatc
    23581 accagattga acgtggcaac ccgcgacgaa cgtttacagg gatggctttt atcgaaggat
    23641 aatgaaggat gaaactgccg gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt
    23701 acttcgccgg aatttctttc agcagttcag tcagcagtgt ccagtaatga cctacgcttt
    23761 cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat agttggcccg atagaaacca
    23821 tgtccatttc cggatacggt tttttgaaca gaccacattc caggcccgcg tggataatct
    23881 ggatgttcgg cgtcttgttg aacaggcgct gataggtttc acgtaccaga tgcatcaccg
    23941 gagaattagc gtccggctgc cagccaggat atgcgccttt cgcttcggtt ttcgcgccag
    24001 ccagtttacc cagcgaatcc agcatgctca ccacgtagtc tttaccgctg tcgatcagtg
    24061 aacggatcag gcagtgaatt tctacgttat tgtcagtcat ggtcaccaca ccgacgttca
    24121 gggaggtttc gaccacacct tttgccacgt cggaattgcg gatcacaccg ttcggggtgg
    24181 cgttcagcag acgaataaag gtatcgcgag atttcgcaat cagggcagct ttatcgttcg
    24241 ctacagagtc cagcaacaag gccagatttt tctctttctc tgccagctcg tttttcagga
    24301 tctcctgata ggtattcacc agagatttca gtgcgtcgac tttatcagct gcgacagcaa
    24361 tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt gccgccgttg aaatcgataa
    24421 ggcgcaggtc cagttcttct gcatgacccg ccaggaagcg caccagcagt ttgttggcat
    24481 tacccaggcc aacgtggatt tcaccgccgg agtgaccgcc tttcagacct tttaaggtta
    24541 acttgaaggt ttcaaaacca gctggaaccg cttcacgatc taaatgcagg ttggaggtga
    24601 agtcgatacc ccccgcacaa cccatgtaga tttcaccttc ttcttcggag tcggtgttaa
    24661 tcagaatatc agcctgcaac cagttgctct gtaagccgaa cgcaccgtcc ataccggctt
    24721 cttcggtcat ggtcagcagc acttccagcg ggccgtgaac cacgttttcg tcagccagaa
    24781 ccgccagtgc agaggccata ccaatgccgt tatccgcacc cagcgtggtg ccgcgcgctt
    24841 taacccattc gccatcaata taaggctgga taggatcttt cgtgaagtca tgcacggtgt
    24901 cgttattttt ctgcggcacc atatcgaggt gggcctgtaa gacgaccggt ttacgatttt
    24961 ccatacctgc ggtagcaggt ttacgaatca ggatattacc tacctgatcg cgttcgacat
    25021 ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc gagttgctct tcatgatagg
    25081 acgggtgagg aatagaacag attttggcaa aaatatccca cagcggctgt ggagataatt
    25141 gagacagttc agacacgtta agtctccttg tcgatcaccc gcaaaacagt attgcaggtc
    25201 acagggttag cagaaaatgt tgtcaacaca agacaggctt gcgagatatg tattgagaat
    25261 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
    25321 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
    25381 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
    25441 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
    25501 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggggcgt tactggcgcg
    25561 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
    25621 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
    25681 tgacctggtg gataccggtg gtactgctgt tgcgattcgt gaaatgtatc caaaagcgca
    25741 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
    25801 tatcccgcaa gatacctgga tcgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
    25861 aatctccggt cgctaatctt ttcaacgcct ggcattgccg ggcgttgttc tttttaactt
    25921 caggcaggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
    25981 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
    26041 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc tcttgatggt aaaaccatcc
    26101 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggtatt gacccacgtg
    26161 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc tgaacgtacc gacgatgatt
    26221 tatacgatac cgtgattggc taccgtggtg gcaactggat ttatgagtgg gccacccagg
    26281 cgatggtgtg gcaacaaaaa gcctgtacgg aagaagatcc gcaactcagt ggtcgacact
    26341 ggctgcatgc ggctacgttg tacaacattg ccgcctatcc tcatctgaaa ggagatgacc
    26401 tggccgagca agcgcaggct ttatcaaacc gcgcctatga agaggccgcc cagcgtctgc
    26461 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
    26521 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
    26581 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
    26641 tgctgactat tgatatgccg tcggtgggct tttcttcaaa atggaagctc acccaggact
    26701 ccagcctgtt gcatcagcac gtcttaaagg cgctgcctaa cgtaccgtgg gtggatcaca
    26761 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatacc
    26821 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
    26881 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
    26941 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
    27001 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
    27061 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
    27121 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
    27181 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaattttgc taatttggta
    27241 aaacagttgc atcacaacag gagatagcaa tgacgttacc gagtggacac ccgaagagca
    27301 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
    27361 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
    27421 gtgaattctg gggctggtgg atggagctgg aagcgcagga atcccgtttt acctacagct
    27481 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagatactg
    27541 aagtggttga acgactggag cacaccctgc gtgagtttca cgagaagctg cgtgaactgc
    27601 tgacgacgct gaatctgaag ctggaaccgg cgaatgattt tcgtgatgaa ccggtgaagt
    27661 taacggcgtg agtgaaatgt gccggatgca tcacatccgg caatattcat taaaactgat
    27721 acgtcatccc aaccgcgaca gtatcatcat tattaatatt cagcttatta tcgctatcca
    27781 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac
    27841 cgacgtcgat ataattgacc agatcttcat caccgatgcc ctcaatatct ttccctttcg
    27901 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
    27961 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
    28021 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
    28081 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
    28141 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
    28201 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
    28261 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
    28321 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga
    28381 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
    28441 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttttgc tgtgcagtat
    28501 cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat
    28561 cgttaatttg agtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac
    28621 tggcgttatc actcatataa tgcatggctt taactttgcc atagacatcc agtttattac
    28681 cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca
    28741 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
    28801 agcgggagag ccccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt
    28861 acagattaat gatatt
//
LOCUS       DAOFMX010000055        28436 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.055, whole genome shotgun sequence.
ACCESSION   DAOFMX010000055 DAOFMX010000000
VERSION     DAOFMX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.055"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            563..1333
                     /locus_tag="QBA20_002632"
     CDS             563..1333
                     /locus_tag="QBA20_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDQ6293116.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1324..1797
                     /locus_tag="QBA20_002633"
     CDS             1324..1797
                     /locus_tag="QBA20_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDQ6293117.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1904..2443
                     /gene="dnaT"
                     /locus_tag="QBA20_002634"
     CDS             1904..2443
                     /gene="dnaT"
                     /locus_tag="QBA20_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDQ6293118.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2446..3183
                     /gene="dnaC"
                     /locus_tag="QBA20_002635"
     CDS             2446..3183
                     /gene="dnaC"
                     /locus_tag="QBA20_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDQ6293119.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3232..3726
                     /locus_tag="QBA20_002636"
     CDS             3232..3726
                     /locus_tag="QBA20_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDQ6293120.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3980..6271
                     /gene="opgB"
                     /locus_tag="QBA20_002637"
     CDS             3980..6271
                     /gene="opgB"
                     /locus_tag="QBA20_002637"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDQ6293121.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFNGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6409..7431)
                     /gene="lgoD"
                     /locus_tag="QBA20_002638"
                     /pseudo
     CDS             complement(6409..7431)
                     /gene="lgoD"
                     /locus_tag="QBA20_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7570..8484
                     /locus_tag="QBA20_002639"
                     /pseudo
     CDS             7570..8484
                     /locus_tag="QBA20_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            8699..10060
                     /locus_tag="QBA20_002640"
     CDS             8699..10060
                     /locus_tag="QBA20_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6293122.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="QBA20_002641"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="QBA20_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDQ6293123.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="QBA20_002642"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="QBA20_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HDQ6293124.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="QBA20_002643"
     CDS             12610..13899
                     /locus_tag="QBA20_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HDQ6293125.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="QBA20_002644"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="QBA20_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HDQ6293126.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQDYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="QBA20_002645"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="QBA20_002645"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HDQ6293127.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="QBA20_002646"
     CDS             16225..16605
                     /locus_tag="QBA20_002646"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HDQ6293128.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..17476
                     /gene="hpaH"
                     /locus_tag="QBA20_002647"
     CDS             16673..17476
                     /gene="hpaH"
                     /locus_tag="QBA20_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HDQ6293129.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17487..18290
                     /gene="hpaI"
                     /locus_tag="QBA20_002648"
     CDS             17487..18290
                     /gene="hpaI"
                     /locus_tag="QBA20_002648"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HDQ6293130.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18312..19667
                     /gene="hpaX"
                     /locus_tag="QBA20_002649"
                     /pseudo
     CDS             18312..19667
                     /gene="hpaX"
                     /locus_tag="QBA20_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
     gene            19677..20567
                     /gene="hpaA"
                     /locus_tag="QBA20_002650"
     CDS             19677..20567
                     /gene="hpaA"
                     /locus_tag="QBA20_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HDQ6293131.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20594..21531
                     /locus_tag="QBA20_002651"
                     /pseudo
     CDS             20594..21531
                     /locus_tag="QBA20_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21758..23316
                     /gene="hpaB"
                     /locus_tag="QBA20_002652"
                     /pseudo
     CDS             21758..23316
                     /gene="hpaB"
                     /locus_tag="QBA20_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23334..23846
                     /locus_tag="QBA20_002653"
     CDS             23334..23846
                     /locus_tag="QBA20_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HDQ6293132.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24239..26389
                     /gene="btsT"
                     /locus_tag="QBA20_002654"
     CDS             24239..26389
                     /gene="btsT"
                     /locus_tag="QBA20_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDQ6293133.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26439..26642
                     /locus_tag="QBA20_002655"
     CDS             26439..26642
                     /locus_tag="QBA20_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDQ6293134.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26653..27609
                     /gene="yjiA"
                     /locus_tag="QBA20_002656"
     CDS             26653..27609
                     /gene="yjiA"
                     /locus_tag="QBA20_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDQ6293135.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27678..>28436)
                     /locus_tag="QBA20_002657"
     CDS             complement(27678..>28436)
                     /locus_tag="QBA20_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDQ6293136.1"
                     /translation="NYTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMART
                     EDFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQ
                     HADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQ
                     ALVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSF
                     YLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6961 a         7429 c         7129 g         6917 t
ORIGIN      
        1 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
       61 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
      121 atgataacct ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttagaaa
      181 tacttaatga aaaacagaaa tctcattcat ttaatttata attaaatgat atctcttccc
      241 gcagaatgcg ggaatcacac aatcaatatt tattcaataa aatctgatga ttttaacttg
      301 ttaacactaa aaaacgcgcc cagctatcta ttatcctgac ccgtaaaggc taaacaataa
      361 attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      421 ttatcaccga gcttgtccca ataaccagga ttaatcagcg caattttgat cttaacctac
      481 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      541 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg
      601 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      661 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa
      721 cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      781 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      841 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc aaccattacg
      901 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      961 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta
     1021 tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc
     1081 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
     1141 caccattgcg atggccgcca gcgttctgct gctagtgccg ggctttccgt tgattaatgc
     1201 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
     1261 tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg
     1321 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1381 cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg
     1441 ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt
     1501 gagtggtcaa cctttatggc ttctatgctg gtcggtacca ttggtattca atggtcgcgc
     1561 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1621 atatcggctt ataccgcaat gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa
     1681 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1741 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1801 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1861 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1921 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1981 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     2041 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga
     2101 tgtcgctctg gacgaccaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     2161 ggggaaattc gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc
     2221 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     2281 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     2341 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2401 cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2461 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2521 ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2581 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2641 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2701 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2761 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2821 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     2881 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2941 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     3001 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     3061 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     3121 agtttatggg tgatcttcaa ctgggatagc taccgtagcc gggtaacggg taaagagtat
     3181 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     3241 actattaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     3301 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3361 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga
     3421 aaccaagcct taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg
     3481 aagcaaaagc tggcttcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3541 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg tattcagggt
     3601 ttgctgaaaa caaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     3661 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     3721 tcctgaaccg cgaaacgccg cgttttagct gttacccggc tgacgcggct ctctcctgcc
     3781 cttttccctg ctcctggacg gttttccccc tgttacacgg gaagcgaccg cgatcaaaaa
     3841 agtctgttta taaactaatt cctaacaaca tcgcattttt ttgacactag aattgcagca
     3901 atatggcgac gccattacat tgtgcttctc aaaaagctta aacgttggct aaacggcccg
     3961 gtttagtgag gatcgcccat tgtcagaact actctctttc gccctttttc tcgcctctgt
     4021 gctgatttac gcatggaaag cgggacgtaa cacctggtgg tttgcagcca cgttaacggt
     4081 gctggggcta tttgtcgttt taaatatcac cctgtttgcc agcgactatt ttactggcga
     4141 tggtattaac gacgcggttc tctatacctt aaccaacagc ctgaccggtg ctggcgtcag
     4201 caaatacatc ctgccgggta tcggcattgt gctggggctg acggcggtgt tcggtgcgct
     4261 gggctggatc ctgcgccgtc gtcgtcatca tccgcaccat tttggttaca gcctgctggc
     4321 gctcttactg gcgctgggtt cggtggacgc cagcccggca tttcgtcaga taacggaact
     4381 ggtgaaatcc cagtcacgcg atggcgaccc ggactttgcg gcttattata aagagccgtc
     4441 gaaaactatc cctgacccga aactcaacct ggtttatatc tacggcgaaa gtctcgagcg
     4501 gacctatttt gataacgaag ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa
     4561 tgaaggcctg gatttcagcc acacgcagca gctgccagga acggattaca cgattgcggg
     4621 catggtggct tctcagtgcg gcataccgct gttcgccccc tttgaaggca acgcctccgc
     4681 ctctgtctcc agcttcttcc cgcagaacat ctgtctgggc gatatcctga aaaactcggg
     4741 ttatcagaac tatttcgtgc agggcgcaaa tctgcgtttt gccggtaaag atgtgttcct
     4801 gaaatcgcac ggcttcgacc acttatacgg ctcagaagag ctgaaaagcg tggtagccga
     4861 cccgcactat cgcaacgact ggggattcta cgacgatacc gttctcgatg aagcgtggaa
     4921 aaagtttgaa gagctttccc gctcaggtca gcgattctca ctgtttaccc tgacagtcga
     4981 tacccatcac ccggatggtt ttatctcgcg cacctgtaac cgcaaaaaat atgattttaa
     5041 cggtaagccg aatcagtcat tcagcgcggt aagttgcagc caggagaata tcgcgacgtt
     5101 tatcaacaaa atcaaagcgt caccgtggtt taaagatact gtcatcgtcg tctcttctga
     5161 ccatttagcg atgaacaaca cggcgtggaa atacctcaat aagcaggatc gcaataacct
     5221 gttttttgtc attcgtggcg acaagccgca gcaaaagacg ctggcagtga agcgtaacac
     5281 gatggataac ggcgcgacgg tgctggacat tctcggtggc gataactatc tcggacttgg
     5341 tcgtagcagt ttatccgggc agtcgatgtc ggaaatcttc ctcaatatca aagagaaaac
     5401 attggcgtgg aagccagata tcatccgcct gtggaaattc cctaaagaga tgaaagagtt
     5461 caccatcgac cagcagaaaa acatgattgc cttctcgggt agccatttcc gtttgccgct
     5521 gctgttgcgg gtttcagaca aacgcgtgga accgctgccg gaaagcgaat actcagcacc
     5581 gctgcgtttc cagctggccg atttcgctcc acgcgacaat ttcgtctggg ttgacagttg
     5641 ctacaagatg gcacaactct gggctccgga actggcactt tccaccgact ggtgtgtctc
     5701 gcaggggcag cttggcggtc agcaaattgt tcagcatgtt gacaaaacaa catggaagag
     5761 caagacggca tttaaagata cggtcatcga catggcgcgt tacaaaggca atgtcgatac
     5821 gctgaagatt gttgataacg atattcgcta caaagccgac agtttcatct ttaacgtcgc
     5881 cggtgcgcca gaagaggtga aacagtttag cgggatttcc cgtccggagt cgtggggccg
     5941 ctggtccaac gcgcagctgg gcgatgaagt aaaaatcgag tacaagcatc cgctgccgaa
     6001 gaaatttgac ctggtgatta ccgccaaagc atacggcaat aacgccagcc gtcctattcc
     6061 ggtacgcgta ggcaatgaag aacaaactct tgtgctgggc aatgaagtga ccaccaccac
     6121 actgcatttc gataacccaa ccgatgccga cacactggta attgtgccgc cggaacctgt
     6181 ctcaaccaac gaagggaata tcctcggaca ctcgccgcgt aagctcggga tcggcatggt
     6241 ggaaattaaa gtggtagaac gtgaagggta attcctgact gatgtgtagg tcgcataggg
     6301 tgcgacctac acgccttctc cggcttacaa cacattatta agaaacaaaa aaaacagcgc
     6361 ccaacacttc cccggacgct gcatggcaac tttaataatc tcgccttatc agaaagtaat
     6421 tacaccctta atcaactcac gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt
     6481 ggcgaacgga tagcgatggg ttaacatcat gtcggcagtg attttccctt ccgccatcag
     6541 tcgaccgact ttagcgaaat cttccggcgt ggcgttgcgg ctgcccatca tcgtcgtttc
     6601 ttttttatgg aattccggat cggagaactg caactcacct ttaaacagac cgacaaatac
     6661 caccgtgccg ccgtgacgaa tcagattcac ggtattattc atcgcatgtt gattacctgt
     6721 cgcgtcgatc actttctgcg ccagcgaacc accaaactgc gcccgcagct gagcgtcaaa
     6781 atcttcggct gacgggtcca gcacgggcaa ctccagacgc gttgccacat gttcacggcg
     6841 cgccggactg gtatccgcca ccaccacctg tgcgccatcg gctttagcga ttgccgccgc
     6901 gcccagaccg attggccccg ccccgaccac cagcacctgc tcgccgggag caatggctgc
     6961 gcgacgcacc gcatgagcgc taatagcgaa aggttcaatc aatgcggccg cctgcgggtc
     7021 aataccgtct gcgggcaaaa tgttcgccac cggcacgctc aaatactcac taaaaccgcc
     7081 atcctgatgc acgccaatga ctgaaatttt ttcacagcag ttggtacgcc cgcttttaca
     7141 cgccgggcat tgctgacagg caacataagg gatcacagca acttgctgac catttttaag
     7201 atcagcaata tttttaccca gcccaacaat ctccccacat atttcatggc ctaaaacccg
     7261 tggataacta aaaaatggtt gatttccacc ctaggcatga atatcggtac cgcaaatccc
     7321 gacagactta atttttatta atgcttcatt gtcacccgga ataggtatct cgcgttgttt
     7381 ccagactaat tctttcggct gctggcaaat taaaacattc atcgtagaca tgattcggct
     7441 atccattttt tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg aacggaaaat
     7501 ctgttttgtg aaaacacacg cataaaaatg ttttaaatcg ggttttaatg gactcacaag
     7561 gaggaaaata tgagtcgttc acaaaattta cgccacaatg tgattaacta ggtcattgat
     7621 gatatggcgc ggggtcatat cccttcccca cttccatcgc aaagtgcgct ggcggaaatg
     7681 tacaacatca gccgcactac agtgcgtcat attctcagcc aattacgcga atgcggcgtc
     7741 ttaacgcagg ttggaaacga ctatgttatt gcgcgtaaac ctgaccatga tgacggtttc
     7801 gcctgtacta ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc ttttttcacg
     7861 atgataaacc agcgtcagtt acgccctagg gaaacttttt ctgaactgca actggctcgg
     7921 gccgcgggtg tcagcccggt ggttgtaaga gaatatcttt taaaattcgg ccgttataat
     7981 cttattcaaa gcgaaaagcg cggccagtgg agcatgaagc aattcgatca atcctacgcc
     8041 gagcagctgt ttgagctacg cgaaatgctc gaaacacatt cacttcagca ctttctcaac
     8101 ctgcctgatc atgatccccg ctggctgcag gcgaaaacca tgctagaacg tcatcggcta
     8161 ctgcgtgaca acatcggcaa tagctttcgc atgttctcgc aacttgacag agattttcac
     8221 tccttactgc tttcagctgc cgacaatatc ttttttgatc aatcgcttga gatcatctca
     8281 gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt
     8341 gccgtcgatg aacatatgac cattctcagc gctctgattt gccgcagtga ccttgatgcc
     8401 acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat
     8461 gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgggtgca
     8521 cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgctgt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gaaccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt gggttgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tagaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg ctggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcaatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggcgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccgaccg cctgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacagga ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 ggcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcagcaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaaccgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcgc cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgagct gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aagtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcgt ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcacccgccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccattag
    17461 ctgccgcttt gtttaaggag agaacgatgg aaaacagttt taaagcggcg ctgaaagcag
    17521 gccgtccgca gattggatta tggctggggc tgagcagcag ctacagcgcg gagttactgg
    17581 ccggaccagg attcgactgg ttattgatcg acggtgagca cgcgccgaat aacgtgcaaa
    17641 ccgtgctcac ccaactacag gcgattgcgc cctaccccag ccagccagtg gtacgcccat
    17701 cgtggaacga tccggtgcaa atcaaacaac tgctggacgt aggcacacaa accttactgg
    17761 tgccgatggt acaaaacgcc gacgaagccc gtaaagcggt acgcgccacc cgttatcccc
    17821 ccgccggtat tcgcggtgtg ggcagtgcgc tggctcgcgc ctcgcgctgg aatcgcattc
    17881 ctgattacct gcaaaaagcc aacgatcaaa tgtgcgtgct ggtgcagatc gaaacgcgtg
    17941 aggcaatgaa gaacttaccg cagattctgg acgtggaagg ggtcgacggc gtgtttatcg
    18001 gcccggcgga tctgagcgcc gatatgggtt atgccggtaa tccgcagcac ccggaagtac
    18061 aggccgccat tgagcaggcg atcgtgcaga tccgcgaagc gggcaaagcg ccggggatcc
    18121 tgatcgccaa tgagcaactg gcaaaacgct atctggaact gggcgcgctg tttgtcgccg
    18181 tcggcgttga caccaccctg ctcgcccgcg ccgccgaagc gctggcagca cggtttggta
    18241 atagttcttc tatttcgatg gcgaagcaaa acaacaactc tgtctactaa acgtacgatg
    18301 tggagcgcat catgacgacc tctaccctgc aatataaaga taataaaact gttgaaattg
    18361 aacatcgtgt cattaataaa ttatttcgtc gcctaatcat ttttcttttt attctgtttg
    18421 ttttttcgtt tcttgatcgc atcaatatag gatttgccgg actgacaatg ggcaaggatc
    18481 ttggcctgac gtccacaatg tttggcctcg ccgcaacact cttttatgtc acgtacgtca
    18541 tgtgcgggat ccccagtaat atgatgttag cgaaaattgg cgctcgtcga tggatcgctg
    18601 ggatcatggt ggtctgggga attgcttcca cctgcacaat gtttgcaaca agccccgaaa
    18661 cactctatct actgcgtatg ctggttggta ttgctgaggc gggtttttta cctggtattt
    18721 tggtttatct gacctggtgg ttcccggcat atcatcgggc cagagccaat gccttattta
    18781 tgatagcgat gccagtcact atgatgctgg gattgatcct ttcaggctac attctggcct
    18841 tggatggact gtggaatcta aaaggctggc aatggctctt tttgctggaa ggtctgccat
    18901 cggtcattct tggtgtcgtc acctagtttt atctcaacga cacaccagac caggcgacct
    18961 ggcttgatga aaacgaaaaa caggctttaa aaacgatgat tctccgtgaa caagagagtg
    19021 catttgccca tgcatctgtt ccacgctcaa ccttacgtga agtcctgacc ccagccatca
    19081 tgctatatac acttgcttat ttttgcctga ctaatactct gagtgcgatt aatatctgga
    19141 cgccgcaaat cctacagagt ttcaatactg gcagtagcaa catcatgatt ggtttgctgg
    19201 cggccatccc ccaattctgc acaatttttg gcatgatctg gtggagtcgt cgttccgacc
    19261 gtcttaaaga acgcaaaaag catacgattc ttccctacct gtttgccgct gccggatggt
    19321 tactcgcttc tgcgacagat cacagcctca ttcaactgag cagcatcatt atggcctcaa
    19381 tgggatcatt taccgcgatg actatctttt ggacaacacc ggatcaagtt ataagcttgc
    19441 aatcgcgtgc cgttgcgatg gctgtcatca atgctgtcgg caatgtaggt tcagcagtca
    19501 gtccattact tatcggtatt ttgaaagatg caaccggcag ttttagctca ggtttgtggt
    19561 ttgtagcagg attgctggta ctgggcgcgt tagtcatgac ccgaatccct atggggagtc
    19621 gggaacactc tctttcatct gaacttatcg caccaaaacc gcaataagga gtcatgatgt
    19681 ccagtgaagc tatctttgaa catggttatg aaaatattga tttggtccgg gaatatgatg
    19741 cccgctacgc tggtgaagat gtgcattatg aaacatttgc ccggttagcg aatttctttg
    19801 gacgcgatat gcgccctcac tggcacgata gatattttca actccattat ctggtcactg
    19861 gacgcattac tttacaactg gatgagcact tctattctgt gcgcgcaccg ctttttgtac
    19921 taacaccacc atcggtgcca catactttct ttactgatcc tgatagcgat gggcatgttc
    19981 tgaccgtacg tcaggagtta atttggcccc tggtagaaaa gctctggcct ggtagtggag
    20041 atgccgcaat gctacaaggt atctgtctgt cactggaggg aatggaacat acacaggcgg
    20101 cattaaatca ttattggccg ataatatccc tggagtttca acaaaataag caaggacggg
    20161 aaattctatt gcaatcactg gcgcaaagta tttttacaat cctactgcgt gaagcaccac
    20221 caaatgatat ttcaacatgc agcgtgcgcg gcgagatgtg tttattccag aagtttaatc
    20281 ggctgattga cgaaaattat cgccaacatt taatggtccc tgaatatgca acaatgctcg
    20341 gtatgagtga atcacggctt accgagctgt gccgacgttt tgccaatcaa tcgccgaaga
    20401 gattgatttt cgaacgagtg agccgggaag ccagacggct tctgctctat agcgagcaaa
    20461 gcatcaacca gatagccctt gatcttggct acaaagatcc cgcctatttc gcccgttttt
    20521 tcaaccgaat gatgggttgc tcaccaacac aattccgtgg tcgctgacca tttatcaacc
    20581 tgagagactc gacatgaaaa tatctttacg ctcactattt gtggcgtgcc tgattgcgcc
    20641 agtggcattc acctacgcct ccgaaccgca ataaatgaaa attgtactac ttggaacgaa
    20701 aggtggccct tctttattaa aagctgaccg tttaccgatg gcaacggcac ttattattgg
    20761 caataaaacc tggttaattg atgctggtta cggggccagc ttacaactgg taaaaaataa
    20821 tattccactg cgccagattg ataatatttt actcacccat ttacacagtg atcatattct
    20881 ggactatcca tcagtcttaa tgaatgcatg ggctagcggt ctgcaggaca aaactattca
    20941 tgtattcggt ccgccaggca cccgagccat gaccgctgcc agttggcagg tcttcgatcg
    21001 ggacatcaca ctgcgtatgg aagaagaagg aaaacccgat cctcgcaaac tcgttacagc
    21061 tcaagatgtt ggtgaaggcg tagtttataa agatgatacc gtgaccgtga ctgcattaaa
    21121 ggtccctcat tctcctttcc ctgacggtga ggcttttgct tatcgcttcg acacacaagg
    21181 caagcgaatt gtcttctcag gagatacctc gtactttccc ccactggcaa ctttctcaga
    21241 aggcgctgat atacttgtgc atgaggccgt acatgagccc tccgtcgcta agctggcaga
    21301 cagtattggc aatggtaaaa cactggcaaa agccattgct tctcaccata cgactattga
    21361 ggatgtaggc aaaattgcca atgaagcaaa agtgaaaaaa ctcatcctta gccacctggt
    21421 accaatcacc gttccggatg aaacatggaa gcaagaagca cagaaaacta ttcaggccct
    21481 atcgtcgttg gtcatgaaaa tatgacgatt accattccat tcgccccata aaaaattccg
    21541 tccccgttag cccttgttag cggggaatat tatgatttgc cttactgtaa gttgatcacg
    21601 atcgcagttt actatttaac tccaccttgc tgtcgaaaag taccaacgtt cgcctgaaac
    21661 gtctcttccc gacaaccgcg ttcaacgctt caattaaata acaaaaacaa cacacaaaat
    21721 taacaatcgt attccgatta atactgtaga ggtcgacatg aaaccagaag atttccgcgc
    21781 cagtacccaa cgtcctttca ccggggaaga gtatctgaaa agcctgcagg atggtcgcga
    21841 gatctatatc tatggcgagc gagtgaaaga cgtcaccact catccggcat ttcgtaatgc
    21901 ggcagcgtct gttgcccaac tgtacgacgc gctgcacaaa ccggagatgc aggactctct
    21961 gtgttggaac accgacaccg gcagcggcgg ctatacccat aaattcttcc gcgtggcgaa
    22021 aagtgccgac gacctgcgcc agcaacgcga cgccatcgct gaatggtcac gcctgagcta
    22081 tggctggatg ggccgtaccc cagactacaa agccgctttc ggttgcgcac tgggcgcgaa
    22141 tccgggcttt tacggtcagt tcgagcagaa cgcccgtaac tggtataccc gtattcagga
    22201 aactggcctc tactttaacc acgcgattgt taacccaccg atcgtcattt gccgaccgat
    22261 aaagtgaaag acgtttacat caagctggaa aaagagactg acgccgggat tatcgtcagc
    22321 ggtgcgaaag tggttgccac caactcggcg ctgactcact acaacatgat tggcttcggc
    22381 tcggcacaag tgatgggcga aaacccggac ttcgcactga tgttcgttgc gccaatggat
    22441 gccgatggcg tgaaattaat ctcccgcgcc tcttatgaga tggtcgcggg tgctaccggc
    22501 tcgccatacg actacccgct ctccagccgc ttcgatgaga acgatgcgat tctggtgatg
    22561 gataacgtac tgatcccatg ggaaaacgtg ctgatctacc gcgattttga tcgctgccgt
    22621 cgctggacga tggaaggcgg tttcgcccgt atgtatccgc tgcaagcctg tgtgcgcctg
    22681 gcagtgaaac tcgacttcat tacggcactg ctgaaaaaat cactcgaatg taccggcacc
    22741 ctggagttcc gtggtgtgca ggccgatctc ggtgaagtgg tggcgtggcg caacaccttc
    22801 tgggcattga gtgactcgat gtgttcagaa gcgacgccgt gggtcaacgg ggcttattta
    22861 ccggatcatg ccgcactgca aacctatcgc gtactggcac caatggccta cgcgaagatc
    22921 aaaaacatta tagaacgcaa cgttaccagt ggcctgatct acctcccttc cagtgcccgt
    22981 gacctgaaca atccgcagat cgaccagtat ctggcgaagt atgtgcgcgg ttcgaacggt
    23041 atggatcacg tccagcgcat caagatcctc aaactgatgt gggacgccat tggcagcgaa
    23101 tttggtggtc gtcacgagct gtatgaaatc aactactccg gtagccagga tgagattcgc
    23161 ctgcaatgtc tgcgccaggc acaaagctcc ggcaatatgg acaagatgat ggcgatggtt
    23221 gatcgctgcc tgtcggaata cgaccagaac ggctggactg tgccgcacct gcacaacaac
    23281 gacgatatca acatgctgga taagctgctg aaataacgca gcaggaggtt aagatgcaat
    23341 tagatgaaca acgcctgcgc tttcatgacg cgatggccag cctgtcggca gcggtaaata
    23401 ttatcaccac cgagggcgac gccggacaat gcgggattac ggcaacggct gtctgctcgg
    23461 tcacggatac accaccgtcg ctgatggtgt gcattaacgc caacagtgcg atgaacccgg
    23521 tttttcaggg caacggcaag ttgtgcgtca acgtcctcaa ccatgagcag gaactgatgg
    23581 cgcgccactt cgcgggcatg acaggcatgg cgatggaaga gcgttttagc ctctcatgct
    23641 ggcaaaaagg tccgctggcg cagccggtgc taaaaggttc gctggccagt cttgaaggag
    23701 agatccgcga tgtgcaggcg attggcacac atctggtgta tctggtggag attaaaaaca
    23761 tcatcctcag tgcagaaggt cacggactta tttactttaa acgccgtttc catccggtga
    23821 tgctggaaat ggaagctgcg atttaagctc cagccccttc acgcattcgt cgaaggggca
    23881 tttttattgt gatctcaatc acatatctac ccgatgaata attcttgccc aaccgctcac
    23941 cgccattcct gaccgcttac atccctaaaa taaccactca gttatttacc ttactttacg
    24001 cgcacgtatc tctggcaaca tcactgcagg atagcggtca atttacctcc tcaaacgcaa
    24061 cgcaaaccta gaacggcttc ggcctatgat taacaaatta ctctatggat tttggtttgc
    24121 aggaaggcag caagtgagtg aatccccggg agcttacaaa agtaagtgtc agaggtgaac
    24181 gaacgcagct gccgcacctg taagccaaaa gacgacgagt aaaatgccag gttttactat
    24241 ggatacgaaa aaactattca agcacatacc ctgggtgatt ctcggaatca tcggtgcatt
    24301 ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc agcgccctgt ggatcgtggt
    24361 cgcctctgtg tcggtgtatc tggtggcgta tcgctactac agtctgtaca tcgcccagaa
    24421 ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt attaacaacg acggtctgaa
    24481 ctacgttccg accaaccgtt acgtgttgtt tggtcaccac ttcgccgcta tcgccggtgc
    24541 tggtccgctg gtgggaccgg ttctcgccgc gcagatgggc tacctgcctg gcacgctgtg
    24601 gctgctggcg ggggtagtgc tggccggtgc ggtacaggac tttatggtgc tgtttatctc
    24661 ctctcgccgt aacggcgcat ctctcggtga gatgatcaaa gaagagatgg ggccagtacc
    24721 ggggactatc gcgctgtttg gctgtttctt aatcatgatc atcatcctcg ccgttctggc
    24781 gctgattgtg gttaaagccc tggccgaaag tccgtggggt gtcttcaccg tttgctcaac
    24841 cgtaccgatt gcgctgttta tgggtatcta catgcgcttt atccgtccgg ggcgtgtggg
    24901 tgaagtctct gttattggta tcgtgctgct ggttgcctct atctacttcg gtggcgtgat
    24961 tgctcacgat ccgtactggg gtccggcact gacctttaaa gacaccacca ttaccttcgc
    25021 gctgattggc tatgcgtttg tttccgcact gctgccggtg tggctgatcc tcgcaccgcg
    25081 cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc ggcctggcgc tgggtatcgt
    25141 ggtgctgaac ccggaactga aaatgcctgc gatgacccag tacattgacg gtactggccc
    25201 gctgtggaaa ggcgctctgt tcccgttcct gttcatcacc atcgcctgtg gtgcggtatc
    25261 tggcttccac gcgctgatct cttccggtac gacgccgaaa ctgctggcta acgaaaccga
    25321 cgcgcgtttc attggctacg gcgcaatgct gatggagtcc ttcgtggcga ttatggcgct
    25381 ggttgcagcg tccatcatcg aaccgggtct ttacttcgcg atgaacaccc cgcctgctgg
    25441 ccttggcatc accatgccta acctgcatga aatgggtggc gagaacgcgc cgatcatcat
    25501 ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc gtcagctcct ggggcttcgt
    25561 gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt ggtgagcctt ctgtcctgaa
    25621 ccgtgcaggt ggtgcgccga cgctggcggt aggtatcgct cacgtgttcc acaaagtgct
    25681 gccgatggct gacatgggct tctggtatca cttcggtatt ctgttcgaag ccctgttcat
    25741 cctgaccgcg ctggatgcgg gtacccgttc tggccgcttt atgctgcaag acctgctggg
    25801 taacttcatc ccgttcctga aaaaaaccga ctctctggtt gccggtatca tcggtactgc
    25861 gggctgtgtg ggtctgtggg gctacctgct gtatcagggc gtggtcgatc cgctgggcgg
    25921 cgttaagagc ctgtggccgc tgttcggtat ctccaaccag atgctggcag ccgtagcgct
    25981 ggtactgggc accgttgtgc tgattaagat gaagcgcacc caatacatct gggtaactgt
    26041 tgttccggct gtatggctgc ttatctgcac cacctgggcg ctgggtctga aactgttcag
    26101 caccaacccg cagatggaag gcttcttcta catggcaagc cagtacaaag agaagattgc
    26161 taacggtact gacctgacgg cgcagcagat tgccaacatg aaccacatcg ttgtgaacaa
    26221 ctacaccaac gcaggcctga gtattctgtt cctgattgtg gtgtacagca tcatcttcta
    26281 cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa cgtactgaca aagaaacacc
    26341 gtacgttcca atcccggaag gcggcgtgaa gatctcttcg caccactaac cgtgtttagc
    26401 cccgcttcgg cggggctttg ttcttatcag agtgaactat gtttggtaac ttaggacagg
    26461 caaaaaaata tctcggccag gcggcgaaga tgttgattgg tattccagac tacgacaact
    26521 acgtcgagca tatgaagacc aaccatcccg acaagccgta catgagctat gaagaattct
    26581 tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa aggcggtatg cgctgttgtt
    26641 aatggagaga gcatgaaccc gattgcagtt accctactca ccggtttttt aggcgcgggc
    26701 aaaactaccc tgctgcgcca tattcttaac gaacaacacg gctacaagat tgccgtgatt
    26761 gaaaacgaat tcggcgaagt ctccgttgat gatcaactga ttggcgaccg cgccacgcag
    26821 atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct ccaacgagct ggaggacgca
    26881 ctactcgacc tgctggataa tctcgacaag ggcaatattc agttcgatcg tctggtgatt
    26941 gaatgcaccg gcatggccga tcccggcccg attattcaga cctttttctc ccatgagatt
    27001 ttatgccagc tctacctgct ggacggcgtg attgcgctgg tggatgcggt acatgccgat
    27061 gagcagatga accagttcac catcgcccag tcccaggtgg gctacgccga ccgtattctg
    27121 ctgactaaaa ccgacgtcgc aggcgaagca gagaaactgc gtgaacgcct ggcgcgcatc
    27181 aacgcccgcg caccggtcta caccgtcacc cacggcgaca tcgacctggg cctgctgttc
    27241 aacaccaacg gttttatgct ggaagagaac gtcgtcagca ccaaaccgcg tttccacttt
    27301 atcgcggata aacaaaacga tatttcgtca attgtggtgg aactggatta cccggtagat
    27361 atcagcgaag tttcccgcgt gatggaaaac ctgctgctgg aatcggcgga taaactgctg
    27421 cgttacaaag ggatgctgtg gattgacggc gaacctaacc gcctgctgtt ccagggcgtc
    27481 cagcgcctct acagcgccga ctgggacagg ccgtggggcg atgaaaaacc gcatagcaca
    27541 atggtgttta tcgggattca gttgccggag gaagagattc gggcggcgtt tgcgggattg
    27601 aggaagtaag ttcgggaaag atcaactttt tgagagttcc accgcaacat tattacgagt
    27661 ggaactcttt agatatgtca gaccaaccaa agcttcttca attgcgtttt cattttttca
    27721 tgtctcattt tcagttcagc caatccccat tctgaagaat tacaaacatc ttttgttaaa
    27781 taaaaagagc ttttactatt aagtttctca taaatagatt tctttttgat aaaatcatag
    27841 tgttgtgttt cagaattttt ggaaccactt atgagtgtta aattacctat tttatgcagc
    27901 cagtagacat gctcttgagg ttggaatcta ttaatccagt attcattaac taatgcttga
    27961 ggaagaatat gctcaattgt gattctcccc gtataggttt taatcacgct ctcatcttgt
    28021 tgctccatat ctaagcgaag caaaattgct tttattaagt tcacttgatt cggacgaggc
    28081 tcatataaat cttcatccag agcagctata aaacctgaat tatctgcatg ttggtttatc
    28141 tgatttatca cagaatcaaa aggcatatca ttatttattg ctaccagagc agaataacaa
    28201 accatttctc tttggctttt aatttgtttt tttaaccacc catgcatata taccttctca
    28261 aaggcggtaa taaattgaga aaaatcatct aaattaaaat cttctgtacg agccattcga
    28321 ttcatgaatg ccataaccgg aggaatccac tcatctaccc ctaaattact tagcgatgct
    28381 atttttcgtc gtattgaagg gtgttcaaaa tcattctcta aaatcttagt ataatt
//
LOCUS       DAOFMX010000056        28402 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.056, whole genome shotgun sequence.
ACCESSION   DAOFMX010000056 DAOFMX010000000
VERSION     DAOFMX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.056"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="QBA20_002658"
     CDS             complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="QBA20_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HDQ6293137.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            322..1953
                     /gene="dcuS"
                     /locus_tag="QBA20_002659"
     CDS             322..1953
                     /gene="dcuS"
                     /locus_tag="QBA20_002659"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDQ6293138.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1950..2669
                     /gene="dcuR"
                     /locus_tag="QBA20_002660"
     CDS             1950..2669
                     /gene="dcuR"
                     /locus_tag="QBA20_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDQ6293139.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3240..4580
                     /gene="dcuB"
                     /locus_tag="QBA20_002661"
     CDS             3240..4580
                     /gene="dcuB"
                     /locus_tag="QBA20_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDQ6293140.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4658..6304
                     /gene="fumB"
                     /locus_tag="QBA20_002662"
     CDS             4658..6304
                     /gene="fumB"
                     /locus_tag="QBA20_002662"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDQ6293141.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6427..7056
                     /locus_tag="QBA20_002663"
     CDS             6427..7056
                     /locus_tag="QBA20_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDQ6293142.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7195..8616)
                     /gene="melB"
                     /locus_tag="QBA20_002664"
     CDS             complement(7195..8616)
                     /gene="melB"
                     /locus_tag="QBA20_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDQ6293143.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8719..10074)
                     /gene="melA"
                     /locus_tag="QBA20_002665"
     CDS             complement(8719..10074)
                     /gene="melA"
                     /locus_tag="QBA20_002665"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDQ6293144.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10357..11265
                     /gene="melR"
                     /locus_tag="QBA20_002666"
     CDS             10357..11265
                     /gene="melR"
                     /locus_tag="QBA20_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDQ6293145.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11464..13731
                     /gene="adiA"
                     /locus_tag="QBA20_002667"
     CDS             11464..13731
                     /gene="adiA"
                     /locus_tag="QBA20_002667"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDQ6293146.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14056..14817
                     /gene="adiY"
                     /locus_tag="QBA20_002668"
     CDS             14056..14817
                     /gene="adiY"
                     /locus_tag="QBA20_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDQ6293147.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14954..16315
                     /gene="adiC"
                     /locus_tag="QBA20_002669"
     CDS             14954..16315
                     /gene="adiC"
                     /locus_tag="QBA20_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDQ6293148.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKDKFPLI
                     QQT"
     gene            16395..18038
                     /gene="eptA"
                     /locus_tag="QBA20_002670"
     CDS             16395..18038
                     /gene="eptA"
                     /locus_tag="QBA20_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDQ6293149.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18035..18703
                     /gene="basR"
                     /locus_tag="QBA20_002671"
     CDS             18035..18703
                     /gene="basR"
                     /locus_tag="QBA20_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDQ6293150.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18704..19804
                     /gene="pmrB"
                     /locus_tag="QBA20_002672"
     CDS             18704..19804
                     /gene="pmrB"
                     /locus_tag="QBA20_002672"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDQ6293151.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19780..19869)
                     /gene="pmrR"
                     /locus_tag="QBA20_002673"
     CDS             complement(19780..19869)
                     /gene="pmrR"
                     /locus_tag="QBA20_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDQ6293152.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19981..21483)
                     /gene="proP"
                     /locus_tag="QBA20_002674"
     CDS             complement(19981..21483)
                     /gene="proP"
                     /locus_tag="QBA20_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDQ6293153.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21747..22625)
                     /locus_tag="QBA20_002675"
     CDS             complement(21747..22625)
                     /locus_tag="QBA20_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDQ6293154.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22622..24850)
                     /gene="crfC"
                     /locus_tag="QBA20_002676"
     CDS             complement(22622..24850)
                     /gene="crfC"
                     /locus_tag="QBA20_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDQ6293155.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25252..25587
                     /locus_tag="QBA20_002677"
     CDS             25252..25587
                     /locus_tag="QBA20_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDQ6293156.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25745..26188
                     /gene="yjdN"
                     /locus_tag="QBA20_002678"
     CDS             25745..26188
                     /gene="yjdN"
                     /locus_tag="QBA20_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDQ6293157.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26321..27109
                     /gene="phnC"
                     /locus_tag="QBA20_002679"
     CDS             26321..27109
                     /gene="phnC"
                     /locus_tag="QBA20_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDQ6293158.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27134..28150
                     /gene="phnD"
                     /locus_tag="QBA20_002680"
     CDS             27134..28150
                     /gene="phnD"
                     /locus_tag="QBA20_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6293159.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28256..>28402
                     /locus_tag="QBA20_002681"
     CDS             28256..>28402
                     /locus_tag="QBA20_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HDQ6293160.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7295 a         7422 c         6936 g         6749 t
ORIGIN      
        1 cgactttcgt ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg
       61 acatatcgca gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc
      121 gtccagtagc gcctgatcca tgaccttccc tctctcatca gtatgattat cgctataagc
      181 atagccccgg aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat
      241 atagccaatc atttttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa
      301 atttgtcaca caaggaagct gatgagacat tcattgccct accacatatt acgcaaacgt
      361 ccgatgaaat tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta
      421 ttggtggtgc atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc
      481 aacaaggcac tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg
      541 cagaaaaaac cgcaggagag tggcatccag gccatcgcgg aagccgtgcg caaacgcaac
      601 gatctgctgt ttattgtcgt taccgatatg caaagtcttc gctactcaca tcctgaagcc
      661 cagcgtattg gtcagccatt taaaggtgat gacatcctta atgcgctgaa tggcgaagaa
      721 aatgtcgcta tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac
      781 gatgaaaatc ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc
      841 caacagatca atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc
      901 ggactgattg gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa
      961 ccctacgaaa tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa
     1021 ggcgtcgttg ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa
     1081 ttgctgaatt accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg
     1141 tcacaggtgg tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag
     1201 attacgatta aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt
     1261 atcatcggtg ccatttcaac cttcagggac aaaactgaag ttcgtaaact gatgcagcga
     1321 ctcgacggtc tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat
     1381 aaattgcatg tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac
     1441 attctcaaaa cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa
     1501 tctccggtta tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat
     1561 acgctgattt taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg
     1621 acgctgatta ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa
     1681 cccggtggcg aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt
     1741 aacgatgatg gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc
     1801 tcgacaaaag gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat
     1861 ctcggcggca gcatcgccgt ggaatcggaa ccggggattt tcacacaatt ttttgtccag
     1921 ataccctggg acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg
     1981 caatggtcgc ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg
     2041 gaacagcctc gacgctggag aaagctaaag agattatctt caatagcgat gcgcctatcg
     2101 acctgatatt gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc
     2161 tgcataacgc gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa
     2221 ccattaaaga ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt
     2281 cccgctttga agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc
     2341 agtattatga ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg
     2401 atcctcgtcg cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga
     2461 ttgacgcgca tcaggactat gagttttcaa ccgacgagct ggctaacgag gttaacatct
     2521 cgcgtgtttc ctgccgaaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca
     2581 gtatccatta tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact
     2641 actcactgct gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta
     2701 ttaccgtaat gcaaaatacc cctggcacaa gttacatcgt taatcagtaa aacttgtgcc
     2761 agatcaaata taattatccc tccatcatcg ttaaaaatta atatctcttc agatgaacgg
     2821 tgtttttaat ttcaaaacga taacaaaagt taattaacta ttatgtcacc cgcattatgt
     2881 gtatttttac ccacaaatgg gtagatcaga ttaatctata aacctaatga tatctgccct
     2941 gagaacaaaa aatagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc
     3001 tgcgcagtac aaactataaa cccatcgcca gagagttttt ctctctgaaa aagccgctta
     3061 tcacagtgca taaatttgcc gctgctttaa tcagccaata ttcactgtga ggtatttgct
     3121 aaagccggta acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca
     3181 gaacgtgact gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca
     3241 tgttatttac tatccaactt atcataatac taatatgtct gttttatggt gccagaaagg
     3301 gtggtatcgc gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc
     3361 accttcagcc aggtaaacca ccagttgatg tcatgctggt tatcattgcg gtggtggcgg
     3421 catcggcgac cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc
     3481 tgctgcgccg caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga
     3541 ccattctttg cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgtcg
     3601 ccattaagaa caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga
     3661 tggggattat cgccagtccg gtgtcggttg cggtcgtgtc tctggttgcg atgctgggta
     3721 atgtcacctt tgatggtcgc catcttgagt tcctcgatct gctggcaatc accattccat
     3781 cgacgttaat cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg
     3841 ataaagacga agagttccag aaattcatct ccgtaccgga aaaccgtgag tatgtttacg
     3901 gtgataccgc gacgctgctg gataaaaaac tgccgaaaag caactggctg gcaatgtgga
     3961 ttttcctcgg ggcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat
     4021 ccttcggcgg caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg
     4081 gggcgctgat tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagtct
     4141 tccgttccgg tatgatcgcc atcgtggcgg tgtacggtat cgcatggatg gcagaaacca
     4201 tgttcggtgc gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc
     4261 cgtgggcgta tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg
     4321 cgctggcggc gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg
     4381 cttcagcacc ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag
     4441 cgattcagtt tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt
     4501 ttattctgcc ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg
     4561 cgatgtacgg gttcttataa atgcactttg cgtgccgccc gtgactacgc ggcacgccat
     4621 tttcgaataa caaatacaga gttacaggct ggaagctatg tcaaacaaac cctttatcta
     4681 ccaggcacct ttcccgatgg ggaaagacaa taccgaatac tatctactca cttccgatta
     4741 cgttagcgtt gccgacttcg acggcgaaac catcctgaaa gtggaaccag aagccctgac
     4801 cctgctggcg cagcaagcct ttcacgacgc ttctttcatg ctacgtccgg cacaccagaa
     4861 acaggttgcg gctattcttc acgatccaga agccagcgaa aacgacaagt acgtggcact
     4921 gcaattctta agaaactccg aaatcgccgc caaaggcgtg ctgccgacct gccaggatac
     4981 cggcaccgcg atcatcgtcg gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga
     5041 agaagcgctg tcgaaaggcg tctataacac ctatatcgaa gataacctgc gctactcgca
     5101 aaacgcggcg ctggacatgt acaaagaggt caacaccggc actaacctgc ctgcgcaaat
     5161 cgacctgtac gcggtagatg gcgatgagta caaattcctt tgcgttgcga aaggcggcgg
     5221 ctctgccaac aaaacgtatc tctaccagga aaccaaagcc ctgctgactc ccggcaaact
     5281 gaaaaacttc ctcgtcgaga aaatgcgtac cctcggtact gcagcctgcc cgccgtacca
     5341 tatcgcgttt gtgattggcg gtacgtctgc ggaaaccaac ctgaaaaccg tcaaattagc
     5401 aagcgctcac tattacgatg aactgccgac ggaagggaac gaacatggtc aggcgttccg
     5461 cgatgtccag ctggaacagg aactgctgga agaggcccag aaactcggtc ttggcgcgca
     5521 gtttggcggt aaatacttcg cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc
     5581 atcctgcccg gtaggcatgg gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat
     5641 caaccgcgaa ggtatctgga tcgaaaaact ggaacacaat ctaggtcagt acattccaca
     5701 agaactgcgt caggctggtg aaggcgaagc ggtgaaggtc gaccttaacc gcccgatgaa
     5761 agagatcctc gcccagcttt cgcaatatcc ggtttccact cgtttgtcgc tcactggcac
     5821 cattattgtg ggtcgagata ttgcacacgc caagctgaaa gagctgattg acgccggtaa
     5881 agaacttccg cagtacatca aagatcaccc gatctactac gcgggtccgg cgaaaacccc
     5941 tgccggttat ccatcaggtt cacttggccc aaccaccgca ggtcgtatgg actcctatgt
     6001 ggatctgctg caatcccacg gcggcagcat gatcatgctg gcgaaaggca accgcagcca
     6061 gcaggttacc gacgcgtgtc ataaacacgg cggcttctac ctcggtagca tcggcggtcc
     6121 ggcggcggta ctggcgcagc agagcatcaa gcatctggaa tgcgtcgctt atccggagct
     6181 gggtatggaa gctatctgga aaatcgaagt agaagatttc ccggcgttta tcctggtcga
     6241 tgacaaaggt aacgacttct tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa
     6301 gtaacctctt cggcccagcg cctggcagca tgctgccagg tgatccccct gggccacctc
     6361 ttttgcgatt gtaatttcat gcttgctggt gaatagtcag tattttccct gacttgcgaa
     6421 ctcaccatga cccgcacact caagccgtta attcttaaca ccggcgcact ggcgctaacg
     6481 ttaatcctga tttataccgg catttcggcc catgacaaac tcacctggct gctggaagtg
     6541 acaccggtga ttatcgtcgt gccgctacta cttgccaccg ccagacgtta cccgttaacg
     6601 ctgctgctct atacgctcat cttctttcac gccatcatcc tgatggtagg cgggcagtac
     6661 acctacgcga aagtccccgt tggttttgag gtgcaggaat ggctggggtt gagtcgtaat
     6721 ccgtatgaca agctggggca ctttttccag gggctggtgc ctgcactggt ggcacgagaa
     6781 attctcgttc gcgggatgta cgtgcgcgga cgtaaaatgg tggcgtttct ggtctgttgc
     6841 gtggcgctgg cgataagcgc gatgtatgaa ttaatcgagt ggtgggcggc actggcgatg
     6901 ggccagggag cggatgattt tctcggtacc cagggcgacc agtgggacac gcaatccgat
     6961 atgttctgcg cgctgcttgg cgcattaacg acagtgatat tgctcgctcg ctttcattgc
     7021 cgccagctac ggcgctatgg cttgataact ggataacaac gtgcactttg ttgatgccgg
     7081 atgcggcgtg aattccttat ccggtctaca aaatttgcaa atccaaagca ttacattaag
     7141 acgtggcctg ataaacatat cgcatcaggc cattttcgtt agctattgtc cacgtcaggc
     7201 tttcacatca ctcactgcac cgaccggaat atcagcatga acaggctcgg gcggtacttt
     7261 gcgatattta tccagcagat gaatctggat cctgcgcagc gtgtcgccat tgaggcgata
     7321 gaaacggaag tagagaatca gcgttaccat gaaaaacaga gtcggtagag caatcataat
     7381 aaactgcata cctaataggg cttgcgtaga ctgttcaaca ttcggtacat agccaatcat
     7441 ccctaacaca accgcaatga aaaaagccgc aaaggctgaa ccgcccttca ccaccatagt
     7501 ctgcacggag taagcgatgc tttcacagcg tacgtgcagt ttatattcac cgtaatcaac
     7561 ggtatctgcc accatgatga cctgtaatac ccagaaaagc gccgttccca cattcagcaa
     7621 aatacccgca atcacaatga ggacgacgtt gtgataactc attaatgcca ttaacaggag
     7681 aacaccacag cttaacaccg gaagaataga tgctccggcc cataaaatgc gtcgggataa
     7741 tgatttaact aagcgtggga agaatactaa cgtcaccagg ttagcagctc ccgcatacga
     7801 cagataatag gggaacaaat ccgcatcacc gataacatat gagaaataat agatagcaaa
     7861 tccggtaata atgttgctgg ctacattata agcaagagcc atacccaaga ggcatgaaag
     7921 ctgatcgttt ttataaataa gcgcaacgat ggctttaagt gtcagatggc ttccttcagc
     7981 agacgattga ttgtctgacg aaaagacttc atgcacattg cgcagagtga tgatggttga
     8041 aacaataaaa aaggcgatca ataccagagt gaacatctga aagccaaatc cccgatcgcc
     8101 accgccgaca taattaacaa atggtagcgt cacacctgcc gtaacaaagc ctgccagact
     8161 ggcaaaaaaa cgcggataag gaaccagttg ttcgcgttca cgtttatcga gcgtgatggt
     8221 tggaaccagc gaccagaagg gaatatccat aatggtgtaa gtcatgcccc agaggatgta
     8281 ggtcacgcaa acaaagacaa tctgagtagt accttcaaac agatgcgcac taaagaggag
     8341 aaataagatt acagagtttg ccaacgtacc gatcaggatc cagggtttga acttacccca
     8401 tcgcgatcgc gtagcattta caatccatcc cataatcgga tcgttaatag catcccagat
     8461 cctcgccacc agaaacaaag taccgaccaa acccacagac agcccgacga catcggtgta
     8521 gtaatacatg aggtacatat acacaatgcc gatcgcaaaa tccttcccga acgctccaaa
     8581 tccataactg agttttgtag tcattgaaat gctcataggg tatcgggtcg ctgttaccaa
     8641 cgcccgcctt ctgttattgg tatcgtcaga ggtgtgcgcc ggaataaatc ccccggcgca
     8701 gtagcgttta gtcgcgtttt aacggtgcaa ccagcttggc agccagtcgc cgtgggcggc
     8761 aatcaggtcg tcaacaagag catatatttc gtcaatgccc agcacggcgg cagtatgcgg
     8821 gtccatcatc gcggcgtggt aaacacggtc gcgattttcc gtaagaatag cttcggtcag
     8881 cagcgtctgt acgttgatgt tggtttgcat cagggcggcc agatgcgaag gtagcgtacc
     8941 gactttggtc ggctgaatgc cattagcatc aaccagacag gctacttcca cgcaacatcc
     9001 ttgtggcagg ttatcaatca aaccatcgtt acggacgttg ccgtaaatca cactcggctc
     9061 gccagtccag atagcgttca tgattgtgct ggcatattcc cgtgacggtt taatatcaat
     9121 ccgggaggcg tttttatact cctccagctc tttatgccag ttcgccagct gctcgacgca
     9181 gcgtttcggg tactcatcca gcggtacttt ataacgctca atcaaatcct cacgacctag
     9241 cttaataaac cacggcgtat attcggcaaa atgttctgac gattccgtga cgaagtagcc
     9301 cagctttttg aacatttcgt agcgcacaat attctggcag cgagtattac catgaatatt
     9361 tggcttcggt gcctgccctg cttcataagc cgccagcagt tccgggtaga gattcacgta
     9421 actgccgtcg gcggttttgc gctccagctc caggtaaaac gccatatggt tgatacctgc
     9481 gcaacggtaa cgcagcgtag ccgggtcgat attgaggtca cgcgccagct cttccgccgt
     9541 tccctgcacc gaatggcaca gcccgacctg tttgatatac ggatagcggg catacatcgc
     9601 ccaggtattc atcgccattg ggttaacata gttgagcatg gtggcatcgg ggcagacttc
     9661 cgtcatgttc tcgcaaattt gccacagatg cggaatggta cgtagcgcgc gcataatacc
     9721 gcccggcccc aacgtatcgg caatggtttg ttccagacca tgccgcttac agacctcgaa
     9781 atcagtcacc gtgcaaggtt cataaccgcc aatctgaaat gccaccacga caaaatcggc
     9841 atcctctaag gcttctttct gttgggtgtg gcaggtgatt ttgccgctgg cccctgctga
     9901 atccatcagc ttacgcacca caatatgcga ctcttccagg cgggtggggt caatgtccat
     9961 cagggcaata tgcgccgttt tcagcgcctc gcgatggaac acatcaccaa gaatattttt
    10021 aacgaaaatc gtcgaaccag cgccgataaa tgtaattttg ggtgcagaca tcatgcagat
    10081 ctcctggctt gcttgaataa cttcatcatg gcaggcgaat ctgcagaaaa cctccggctt
    10141 ccaggttgag cattcccata aactcagatc ctgcgtgaag cagcagtaaa tctgagttta
    10201 tgggaataaa ttacctgaaa agtagagttt tcggcctcat gatgctcgga cagtcccctc
    10261 gcccctctgg gaagagggtg aaaaccgtgc tcccactcgc agtcatcctc cctcactcct
    10321 gccataattc tgatattcta ggaaagagag ccatccatga atacagatac gtttatgtgc
    10381 agcagcgacg aaaaacagac ccgcagcccg ctgtcgctgt attcggaata ccagcggatg
    10441 gagattgagt ttcgcgcacc gcatatcatg cccaccagcc actggcatgg tcaggtcgaa
    10501 gtgaatgtgc ctttcgatgg cgatgtggaa tacctgatca acaatgaaaa agtgaatatc
    10561 aatcagggtc atatcacgct gttctgggcc tgtacaccgc accaactaac agataccgga
    10621 acctgtcaga gcatggcgat ttttaatctg ccgatgcatc tgtttctctc ctggccgctg
    10681 gataaagacc tgattaacca cgtcactcac ggcatggtga tcaaatcact tgccacacag
    10741 caacttagcc cgtttgaagt acgccgctgg cagcaggaat taaacagtcc gaatgagcaa
    10801 attcgccagc tcgccattga tgaaattggc ctgatgctca agcgatttag cctctctagc
    10861 tgggaaccga ttctggtcaa taaaacctcg cgcacacaca aaaacagcgt ctcgcgccat
    10921 gcgcaatttt atgttagcca gatgctgggc tttattgccg aaaactatga tcaggcgctg
    10981 accatcaacg atgtggctga gcacgtcaaa cttaacgcca actatgcaat ggggatattc
    11041 cagcgggtca tgcaattgac gatgaaacag tacattaccg cgatgcgcat caaccacgtt
    11101 cgcgcgttac tgagcgatac cgataaaagt attctcgata ttgccctgac ggcaggcttt
    11161 cgttcaagta gtcgttttta cagcacgttc ggcaaatatg tcggcatgtc gccgcaacaa
    11221 taccgcaaac ttagccaaca gcgccgccag acgtttcccg gctaaagacg atatcagtat
    11281 cagccaaaaa aatagtttag ccgtcgataa taagcagtga aaataattta aaataatcac
    11341 ataacgtatt gtttataaaa ctaaatattt caaaaatgtt tgtttttcac gcgctttaca
    11401 gcccgaaaag gccggaagat acttgcccgc aacgaagatt ccttcataac cgggtaagca
    11461 atgatgaaag tattaattgt tgaaagcgag tttctccatc aagacacctg ggtcggtaac
    11521 gccgttgagc gtctggcaga tgctttaagc cagcaaaatg ttaccgtgat taaatccacc
    11581 tcctttgatg atggttttgc cattctctct tcaaacgaag ccattgactg cctgatgttc
    11641 agctatcaaa tggagcatcc ggacgaacat caaaacgtca gacaattgat cggtaagctt
    11701 catgagcgcc aacaaaacgt gccggtcttc ctgttgggcg atcgggaaaa agccctcgcc
    11761 gcaatggacc gggatctgct ggagcttgtc gatgaattcg cctggattct ggaagatacc
    11821 gccgacttta tcgccggacg cgccgttgcc gcgatgaccc gctaccgcca gcagctgttg
    11881 ccgccactgt tcagcgcgct gatgaaatat agtgacatcc atgaatattc ctgggcagcg
    11941 ccaggccacc agggcggcgt tggttttacc aaaacacccg ccggacgttt ctaccatgac
    12001 tactatggtg aaaatctgtt ccgcaccgac atgggcatcg aacgaacttc cctcggttct
    12061 ttgcttgacc atactggcgc atttggcgaa agcgaaaaat atgccgcacg cgtatttggt
    12121 gccgatcgct cctggtcggt agtcgtcggt acttccggct ctaaccgcac catcatgcag
    12181 gcttgcatga ccgataacga tgtcgtggtc gttgaccgta actgccataa atccatcgaa
    12241 caaggtttga tgctgacagg cgcgaaaccg gtctatatgg tgccaagccg caaccgctac
    12301 ggcattatcg ggccaatcta tccgcaggaa atgcaacctg aaaccttgca aaagaaaatc
    12361 agtgaaagcc cgctgaccaa agacaaagcc gggcaaaaac cgtcttactg cgtggtaacc
    12421 aactgcacct atgacggcgt gtgttataac gctaaagaag cgcaggatct gctggaaaaa
    12481 acctccgatc gtctgcactt tgacgaagcc tggtacggct atgcacgttt caacccgatc
    12541 tatgccgatc actatgccat gcgcggcgaa cctggcgatc acaacggtcc taccgttttc
    12601 gccacccact ccatccacaa actgctgaat gcgctgtcac aggcttctta tattcatgta
    12661 cgtgaaggtc gtggggcgat taacttctcc cgcttcaacc aggcctacat gatgcatgcc
    12721 accacctccc cgctgtatgc catctgcgca tccaacgacg tggcggtgtc gatgatggac
    12781 ggcaacagcg gcctgtcact gacacaggaa gtgattgacg aagcggttga tttccgtcag
    12841 gcgatggcgc ggctatataa agagttcacc gctgacggta gctggttctt caaaccgtgg
    12901 aacaaagaag tcgtcaccga cccacaaacc ggcaaaacct atgactttgc tgacgcacca
    12961 accaaactgc tgaccaccgt tcaggactgc tgggtaatgc atccgggcga aagctggcac
    13021 ggcttcaaag atattccgga taactggagt atgctcgacc caattaaagt cagcatcctt
    13081 gctccgggaa tgggtgaaga tggtgaactg gaagaaaccg gtgttccggc ggcgctggtc
    13141 actgcctggc ttggtcgcca cggcattgtg cctacccgca ccactgactt ccaaattatg
    13201 ttcctgttct ctatgggcgt aacccgtggg aaatggggga ctctggttaa caccctttgc
    13261 tccttcaaac accactatga cgccaacaca ccgctggcgc aggtgatgcc ggaacttgtt
    13321 gaacaatatc ctgacactta cgcgaacatg gggattcacg atctgggtga caccatgttt
    13381 gcctggctga aagaaaacaa ccctggcgca cggttgaacg aagcctattc cggcctgccg
    13441 gtggcggaaa tcaccccgcg tgaagcgtac aacgcgattg tcgacaacaa tgtcgaactg
    13501 gtatccattg aaaatctgcc aggacgcatc gcggcaaact cagttatccc gtatccgcca
    13561 ggaatcccga tgctgctgtc tggtgaaaac ttcggcgata aaaacagtcc gcaagtaagt
    13621 tatttacgct cgctgcaatc ctgggaccac catttccctg gatttgaaca cgaaactgaa
    13681 gggactgaaa ttattgacgg tatttaccac gttatgtgcg tgaaagcgta accactattc
    13741 cgctgaaggc gtaattgttt aaataacatt acgccgcctg gccttaggcc ttttgagtat
    13801 ggcaacgttt tcataaaaat tgctgcaaac aaaaatgtca tactttttgc gcggccccac
    13861 cccgcgcttt tttttgcctg ttatttatcc tgtaaaaaaa tatgtacatg agaaattact
    13921 ataaaaattt gtaatattag taaaactcgt tatttttatg catgtttata ttcatcatgc
    13981 aattatataa ccatttcccg gtatcgcttt gctttagcga gaactggtct tttattcgca
    14041 ctcaggagta catgtatgag gatttgcagc gaccaacctt gtattgtttt attgactgaa
    14101 aaagatgtct ggataagggt gaatgggaaa gaacctatta gccttaaagc taaccatatg
    14161 gcgttattaa attgtgaaaa taatattatc gacgtctcct ctcttaacaa cactttggtt
    14221 gctcatatta gtcacgacat catcaaagat tacctccggt ttctgaataa agatctctcg
    14281 caaataccag tatggcaacg tagcgctacg cccatactca ccctgccatg cctgacgcca
    14341 gacgtctttc gcgttgccgc gcaacacagc atgatgcccg cagaaactga gtcagaaaag
    14401 gaacgaacac gcgcattatt attcacggtg ctatcccgtt ttctcgacag taaaaaattc
    14461 ctttcattaa tgatgtatat gttacgtaat tgtgtaagtg acagtgttta tcaaattatt
    14521 gaaagcgata ttcacaaaga ctggaatctt agtatggtag ccagttgttt atgtcttagc
    14581 ccaagtctgt taaagaaaaa gttgaaaagc gaaaacacca gttatagcca aataatcacc
    14641 acctgccgca tgcgttatgc cgtaaatgaa ttaatgatgg acggtaaaaa tatctctcag
    14701 gtatcacagt cctgcggcta caacagtacg tcgtacttta tttctgtctt taaagacttc
    14761 tacggtatga cgccgctgca ttatgttagt cagcacagag aacgcactgt cgcctgattt
    14821 ttaaccttaa cgaagagcta tattaataac ggcatcagcg ataacccggt cgataataat
    14881 tcaactatcg aatgcaggcg tatgatatga cgtaatttat tgtcacgaag ctcgccttcg
    14941 caggagttta attatgtctt cggatgctga tgctcacaaa gtgggcttaa tccccgtcac
    15001 cctgatggtg tcggggaata ttatggggtc aggtgttttt ctgttacctg caaacctggc
    15061 ctccactggc gggattgcca tttatggatg gttggtgacg attatcggtg cgctggggct
    15121 ctcgatggta tacgccaaaa tgtcgttcct cgacccaagt cctggtggtt cttacgctta
    15181 cgcccgccgc tgctttggcc cgtttctcgg ttatcaaacc aacgtcctct actggctggc
    15241 ctgctggatc ggcaatatcg ccatggtggt cattggcgta ggatatttaa gttacttctt
    15301 cccgattctg aaagatccat tggtattaac catcacctgc gtcgtggtgc tgtggatctt
    15361 cgtcctgctg aacattgtcg gtccgaaaat gatcacccgc gtgcaggccg ttgccactgt
    15421 gctggcgctg atccccatcg tcgggattgc cgtatttggc tggttctggt tccgtggtga
    15481 aacctatatg gcggcatgga acgtcagcgg cctgggcacc ttcggtgcga ttcaaagtac
    15541 ccttaacgtt acgctgtggt cgttcatcgg tgtggaaagt gcctccgttg ccgcaggtgt
    15601 ggtgaaaaac ccgaaacgca atgtccctat cgccaccatt ggtggggtat tgattgccgc
    15661 cgtttgctat gtgctctcca ccaccgcgat tatggggatg atccctaatg ccgcactgcg
    15721 cgtttctgcc tcgccattcg gtgatgccgc acggatggcg ttgggtgaca ccgctggggc
    15781 cattgtttcc ttctgcgcag ctgcgggttg cttaggttca ctgggcggct ggacgttgct
    15841 ggcgggtcaa acggcgaaag ccgctgccga tgacggactg ttcccaccga tttttgcccg
    15901 tgtaaataaa gcgggtacgc cagtggcggg gctgattatc gtcggtattt tgatgaccat
    15961 cttccagctc agcagcattt caccaaacgc gaccaaagag ttcggtctgg tttcttccgt
    16021 ctcggtcatc tttacactgg tgccatatct ttacacctgt gcggcgttac tgctgctcgg
    16081 ccacggtcac tttggtaaag cacgcccggc atatctggct gttactacca ttgccttcct
    16141 ctactgcatt tgggccgtgg tggggtccgg ggcgaaagag gttatgtggt catttgtcac
    16201 cctgatggtc atcaccgcca tgtatgcgct gaattacaac cggctacata aaaacccgta
    16261 tcccttagat gcaccaataa gcaaagataa attcccctta atccagcaaa cataaaagcc
    16321 aaccttaaga acttaaggtt ggcttaattt tgctttgcga gcatatgcgc actttgttcg
    16381 atggaaacac cgtgatgttg aagcgcctac taaaaagacc ctctttgaat ttactcgcct
    16441 ggctattgtt ggccgctttt tatatctcta tctgcctgaa tattgccttt tttaaacagg
    16501 tgttgcaggc gctgccgctg gattcgctgc ataacgtact ggttttcttg tcgatgccgg
    16561 tcgtcgcttt cagcgtgatt aatattgtcc tgacactaag ctctttctta tggcttaatc
    16621 gaccactggc ctgcctgttt attctgattg gcgcggctgc acaatatttc ataatgactt
    16681 acggcatcgt catcgaccgc tcgatgattg ccaatattat tgataccact ccagcagaaa
    16741 gttatgcgct gatgacaccg caaatgttat taacgctggg attcagcggc gtgcttgctg
    16801 cgctgattgc ctgctggcta aaaatcaaac ctgccacctc gcgtctgcgc agtgttcttt
    16861 tccgtggagc caatattctg gtttctgtac tgctgatttt gctggtcgcc gcactgtttt
    16921 ataaagacta cgcctcgttg ttccgcaata acaaagagct ggtgaaatcc ttaagcccct
    16981 ctaacagcat tgttgccagc tggtcatggt actcccatca gcgactggca aatctgccgc
    17041 tggtgcgaat tggtgaagac gcgcaccgca acccgttaat gcagaacgaa aaacgtaaaa
    17101 atttgaccat cctgattgtc ggcgaaacct cgcgggcgga gaacttctcc ctcaacggct
    17161 acccgcgtga aactaacccg cggctggcga aagataacgt ggtctatttc cctaataccg
    17221 catcttgcgg cacggcaacg gcagtttcag taccgtgcat gttctcggat atgccgcgtg
    17281 agcactacaa agaagagctg gcacagcacc aggaaggcgt gctggatatc attcagcgag
    17341 cgggcatcaa cgtgctgtgg aatgacaacg atggcggctg taaaggtgcc tgcgaccgcg
    17401 tgcctcacca gaacgtcacc gcgctgaatc tgcctgatca gtgcatcaac ggcgaatgct
    17461 atgacgaagt gctgttccac gggctggaag agtacatcaa taacctgcag ggtgatggcg
    17521 tgattgtctt acacaccatc ggcagccacg gtccgaccta ttacaaccgc tatccgccgc
    17581 agttcaggaa atttacccca acctgcgaca ccaatgagat ccagacctgt accaaagagc
    17641 aactggtgaa cacttacgac aacacgctgg tttacgtcga ctatattgtt gataaagcga
    17701 ttaatctgct taaagaacat caggataaat ttaccaccag cctggtttat ctttctgacc
    17761 acggtgaatc gttaggtgaa aatggcatct atctgcacgg tctgccttat gccatcgccc
    17821 cggatagcca aaaacaggtg ccaatgctgc tgtggctgtc ggaggattat caaaagcggt
    17881 atcaggttga ccagaactgc ctgcaaaaac aggcgcaaac gcaacactat tcacaagaca
    17941 atttattctc cacgctattg ggattaactg gcgttgagac gaagtattac caggccgcgg
    18001 atgatattct gcaaacttgc aggagagtga gtaaatgaaa attctgattg ttgaagacga
    18061 tacgctgtta ttgcagggac tgattctggc ggcgcaaacc gaaggctacg cgtgcgatgg
    18121 cgtgacaacc gcgcggatgg cggaacaaag ccttgaggac ggtcattaca gcctggtggt
    18181 actggattta gggttacccg acgaagatgg actgcatttt ctcgcccgta tccggcagaa
    18241 aaaatacacc ctgccggtac tgatcctcac cgctcgcgat acgctgaccg acaaaatcgc
    18301 cgggctggat gtcggtgccg atgactatct ggtgaagcct tttgcgctgg aagagttaca
    18361 cgcccgtatc cgcgccctgc tacgacgcca taataatcag ggcgaaagtg agctgattgt
    18421 tggcaatctg acgctgaata tgggtcgccg tcaggtatgg atgggcggtg aagagttgat
    18481 tctgacgccc aaagaatatg ctctgctgtc acggttaatg ctcaaagcag gcagtccggt
    18541 acatcgggaa attctctaca acgacatcta taactgggac aatgaaccct cgaccaacac
    18601 cctggaagtg catatccaca atctgcgcga caaagtgggc aaagcccgta tccgcaccgt
    18661 gcgcggcttt ggctatatgc tggtcgcgaa tgaggaaaac taattgaatc tgatgcgttt
    18721 tctgcgccga ccaatatcgc tgcgccaacg gctgatattg accatcgggg ccattttgtt
    18781 ggtgtttgag ctgatcagcg tcttctggct atggcatgaa agtaccgagc agattcagct
    18841 gtttgagcag gcgctacgcg acaatcgcaa caacgatcgc cacatcatgc gtgagatccg
    18901 cgaggcggtc gccagcctga ttgtccccgg cgtctttatg gtcagcctga cgctatttat
    18961 ctgctatcag gcggtacgcc gcatcacccg cccgctggcg gagctgcaaa aagagctgga
    19021 agcgcgcacc gccgacaact taacgcccat tgccattcac agcgccaccc tcgaaatcga
    19081 agcggtggtt tcggcgttga acgatctggt cagtcgcctg accagcacgc tggataacga
    19141 aaggttgttt accgctgacg tcgcgcacga actgcgaacg ccactggcgg gggtgcgttt
    19201 gcatctggaa ctgctggcga aaacgcatca cattgatgta gcaccgttag tggcacggct
    19261 tgatcagatg atggagagcg tctcccagct gctgcaactg gcgcgtgccg gacagtcatt
    19321 ttcttccggt aattatcaac atgtaaaact gctggaagat gtcattctcc cctcgtatga
    19381 cgaactcagt accatgctcg accagcgaca gcaaaccctg ctattgccag agagcgccgc
    19441 cgacatcacc gttcagggtg atgcaaccct gctgcggatg ttattgcgaa acctggtaga
    19501 aaacgcccat cgttacagcc cgcaaggcag caacattatg attaagctgc aagaagatgg
    19561 cggagcggtc atggcagttg aagatgaagg accaggtatt gatgagagta aatgcgggga
    19621 gttgagtaaa gcgtttgtac gtatggacag ccgttatggc gggattggtc tggggttaag
    19681 tattgtcagc cgcattacac agttgcatca cgggcagttt ttcctgcaaa accggcaaga
    19741 gacttccggc acgcgggcct gggtacggct gaagaaagat cagaacgtgg caaaccagat
    19801 ataaagaaag ctgctgacca ccagcacgct gaacacggta gttaaacttt cataaacacg
    19861 gtttttcatt gcactctcct ccgttaacct ggaggagagt atgcgcgtca gagattaagt
    19921 gaaccttaag agttcaattg gcctgataag acagcgtcac atcaggccat ccgtttcagc
    19981 ttattcatca attcgcggat gttgctgcac caggcgggta cgtttcgcct gcaaatcggc
    20041 aatctcgtgg tcaatattat cgattttctg ctcgatatta tcgtaatgct cgacgagaat
    20101 ttccttcgct tcctgtatat ctgacgccgc cggtgtcgca cctttcaacg gacgatttgc
    20161 cgtctctttc atggttacgc cggtgattaa accaatcacc gccactacca tcaggtaata
    20221 ggcaggcatc atcagattct gcgagctttc gaccagccag gccgccagtg ttggcgtcag
    20281 accggcaacc agcaccgaaa tattaaatgc cgccgccagc gcgctgtaac ggatatgcgt
    20341 cgggaacatc gctggcaagg tagaagccat aacgcccata aagcagttaa ggatcaccgc
    20401 cagcatcagt aacccggcaa aaatcaggcc gatgacgtta ctgttaatca gaataaacgc
    20461 cgggatcgcc aacacaaaca gtgcaacact accaagtagc acaaacggac gacggccaaa
    20521 acggtcactc agcaagccca tcaccggctg gacaaacagc ataccgatca taatggcgat
    20581 aataatcagc accccgtggt cttcggagta atgcaggtta tgcgacaaat aactcggcat
    20641 ataggtcagc aacatgtagt aagtcacgtt ggtggcaatt accagaccaa tacatgtcaa
    20701 caggctgcgc cagtatttag tggcaatctc tttaaacgag actttcgggc catcctgcaa
    20761 accttcgcgg tcgccctgtt ccagtttatc aacatgctgc tggaacgccg gagtttcttc
    20821 cagcgcatgg cgcaggtaaa gcccgataat ccctaacggc agagcaataa agaacggaat
    20881 acgccagccc cagtcgagga agttcgcttc gccgacaatg gtcgaaatta acaccaccac
    20941 gcccgcaccc agcacaaacc cggcaataga accgaagtcc agccagctgc ccataaagcc
    21001 acgtttacgg tcaggggagt attccgcaac aaatatcgac gccccggtat attcaccacc
    21061 gaccgagaaa ccttgtgcca tcttacagat caacagcaga atcggtgccc aaataccaat
    21121 cgtgtcgtag gacggtatta agccaataca gaacgtactg atcgacataa tcacaatagt
    21181 gatagcgagg atcttctggc gaccatattt atcgcccaac ataccaaaga agagtccgcc
    21241 aagcggtcga atcagaaagg gaacggagaa agtggcaagt gcagcaacca tctgcacgct
    21301 ggggtcagcc cccgggaaaa aaactttacc taatgcgtaa gcaacaaaac cataaacacc
    21361 aaaatcaaac cattccattg cattacccag tgatgctgcg gtaatggctt tacgcagttt
    21421 accgtcatca ataatggtga catcacgaag ggtaatcggt tttacttttt tccttttcag
    21481 catagctttc ctcgcagagt tggcccattt ccgcaaacgc cagaaaaatc agcgcgacgg
    21541 ccgcaaaagg ggagcgggaa ttgcaggatg caatctctgt aacaagcgta gcgggtttgc
    21601 ttacaccctc cggttaagga atgtacaatc aaaccagttg acgcctgaat tgggcagtta
    21661 atgacctttt taccctacca gtgtttaaat ttgtgatcaa cttcacacat ggatttcgtt
    21721 taggactcat tgatgtaact gattatttac gccaaattta cagagaagat ttcctcgacc
    21781 attgccccca ccaaacgctg cgctgaacag agtcgtggca tccccagcgc gacagtttgc
    21841 cagcgttggg tcactgacca gcaaacgggt tgtttatctg ccgtggtcca ctttcccagc
    21901 caacttaaaa aatcagcatg atccccgccc gtcggaacgg tcgaagaggc aaaccactga
    21961 tggtaatagt gacgcgtagt cggtgccgca ttgatactct ctgccagatg ttgtgtcgct
    22021 ttctcgcgca aatcacccag caacaacacc accgcttgcg ggcttcccag acggcaggca
    22081 tcgccaaatg caccccagcg caactcctcc agaacgataa aacagcgccc aggcaacgac
    22141 caggcgttat acgccccagc acgccagcgg gtaaaaattt gctcgctctg atcacctgcc
    22201 tgcaccagta ggcctcgttg cgttaacgcc ttttccttcc agcgacaacg ggctgaaaac
    22261 cactcataca aggcggcaat ttgctggggg agctccggga aagcagagtg ctggcggtga
    22321 tccatttttt gcagcagcgc cagatgctgc gcgatctgcg tcagcgccag atgaccggga
    22381 tccagcatcg acgccagttt ttccaccatt gtcagtcgca ggctaagcgg cgcatttaac
    22441 atgccgccgt tcgtccatgt ctgaagctgc acacgcgaaa gaagttcctg catcagtcgt
    22501 tcacgaaact gctgctgatg ggcttgagga tggcgggcat catcgccctg cgccagatcc
    22561 actaaaaagc gcggatgaac cgactccagc acgcgaccgg ggccgtccag taacgtcttg
    22621 gtcaatatcg ttctgccgtg aaaagtgttt gaatatcatc gcgtaacagc tgagcgtcgg
    22681 tgtaaatcca gctgtgagtc atcacagtct gctgcaattg ctgctgcatc agcctgacca
    22741 ccgattcatt ttgttgacgc agagccagac tttcgcgtaa acgcgtctgt aattctgtca
    22801 aacatgaagc gaactcagcg aaaaaagtat tcatgcctgc cgtaacagat tcatcgacct
    22861 gctctgccag aactttagcc atttgttggc aatagagatc gacttctgcg cttaatgctc
    22921 gttgcaacac actgtaatca accgtttctg tcggggattt ctcatttccc cgtccccagt
    22981 cgggctgatt caaccagcgc gaaaaagtct cacgcacaac gcctaaacgc gtgctctgct
    23041 cgtccgttgc ttcctggcgc gaaatgactt cactgaacag ctggcgagtg ttgaagtggg
    23101 gaactacgcc gtgaaaaaca ggaaaatgaa acccaggacg aaaccctgac tcgctcaatt
    23161 ccattttgac ttgttgctca atggggcgaa taacatcggt taacacccgg caaagggtgg
    23221 attccatctc ggcaaaacgc agcgtaaagt cgcgactgat ggtgttctgc gccgtctgta
    23281 acagtgtctc acagcgggta cgaatctcgc ttaacggctc cgaatcatcc tgaaacaagg
    23341 cggctaactg cgcattcacc gcatcttgct gttgacgcag aaagtggttg gcggaggtca
    23401 gggccagctc gatttcatgt ttaatctcgc cgctcacctg cgcctgattg agttgcaata
    23461 gctgcaaact ttcttcgacc tgatggatat tttgccgcaa ttgttcacaa gcgacgttta
    23521 acccgtgcgc acgaaaatcc aggtattccc gcgcctgctg cgcgtaattc aacagtttat
    23581 gcgcagcaga tcgcagagca tacaatgagg cgttagcgta agcggcatga agcaacgcct
    23641 gaattggctg ggcgaacagc gaatcttccc acaactgatc ggcagcatga cgaatatgtt
    23701 cgaggtccgc cagatcggca tgacgccagc gcctgccgag cgcggcatgg gcaaaatctt
    23761 ccacccagcg ttgttgctct ggcgctggta acttaccgtt gttggctaac tcatagcgcg
    23821 cccgattcgc caggtagccc cacatcgacg acaccgggaa tatctgctgt ggcgtaatac
    23881 agcctttcat cagcgtcccg gaaatcagtg cccgcacctg gtcggcgtcg tcactgttac
    23941 gatcctgttg atcgaactta ttgaccagca catacagcgg caccgattgc cccaccgcca
    24001 aaatcgcctc acggacctct tcatcggaga tcgatttcag ttgcgtataa tccagcaccg
    24061 ccagtaccgc cgaggcgcgt gccagctgct ggttaagcat tttttgcaga tgcggttgcc
    24121 cggcttcatt tggcccgggg gtatccagta acgtcaactg accgggataa ctttccagcc
    24181 ccgccagatg gacaaactcc acttcaatca cgggaatatg ctcaatggcg gcgtaagcag
    24241 aaaaaggaaa atcgacgtcc agcgccttcg ccagtcgcac taaatcattc aaacttttca
    24301 gacaatgaaa aataggctgg gcacccagat aatatttttc gaaagcgacg ccattttcga
    24361 tccgctgcat aagcgcacgc atatctttat ctatttccag cacatcggtc agatgcttaa
    24421 tatcgcaatc acgcaggcgc tgttgtaatt gttgaattaa acaatcgatt ggcgcgacat
    24481 gtgaaaaatg cagtaccggt tccttttgcc cgggcgtatg gcgaataagc gtcggcagcg
    24541 cagtcattgg gcgattacga ttaggcagaa cctccgtacc aacaatggca ttaatggtgg
    24601 ttgatttccc tgctttcatg gtaccgacaa ttgccagcac catttccagt cgggaaattt
    24661 tacacaactc attattcagc atcgcgtgac gttcggcgat attaggctga ctccagggta
    24721 aagccagttg tggcgcgtcg tctccgggta cagagagagg cattttttcc agtaactgca
    24781 actgttggcg agaaagctgt aacaggcgtt cagcctcctg acttaactca tacagggtct
    24841 gtgtgtacat agaaaattct tccttaaagc aaattttgtt acttttattt agccagattg
    24901 tttttgagtt ctgttttcgg cttttataat tactgcaaga aataatttta tatttagtgt
    24961 gttgtttttt atcagaataa ataacgtctt ctgatgcgtt taaaacgtca gaaagataaa
    25021 aatatcatgt gaataaaaaa aagaacaagt agagcattaa cattatctta aataataaat
    25081 agaggcaaaa agattatttt ctttttgcgt ttcctttcaa atgaaaacga tcgtcgtcta
    25141 aaatcagcag tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc
    25201 aatctgccgt tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta
    25261 ccacactgcc caaaatgcaa ctccgaatac acttacgaag ataacggcat gtacatctgc
    25321 ccggaatgtg cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc
    25381 gttaaagatg ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat
    25441 ctgaaggtga aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc
    25501 ctggttgaag gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg
    25561 aaatctgagt ttgtgaaaaa gaactgattg tattgtgatc ggtaagccgg ataaggcgct
    25621 cgcgccgcat ccggcaacga tgccgactgc ctgatgcgac gcttgcgcgt cttatcaggc
    25681 agcctctcat ctgtataaat ttcgaactac acttaactgg cttctcttaa ctgaggtcac
    25741 catcatgccg ttaagtccct acctctcttt tgccggtaac tgttccgacg cgattgccta
    25801 ttatcaacgt acgttgggcg cggaactgct ctataaaatc agcttcggcg aaatgccgaa
    25861 atcagcgcag gacagcgccg agaactgccc ttccggaatg caatttcccg ataccgccat
    25921 cgctcatgcc aacgtgcgca ttgccggaag cgacatcatg atgagcgatg ctataccgtc
    25981 aggaaaagcc agctactccg gctttacgct ggtgctcgat tcgcaacagg tcgaagaagg
    26041 aaaacgctgg tttgataacc ttgccgctaa cggaaaaatc gaaatggcct ggcaggaaac
    26101 tttctgggcg catggctttg gcaaagtcac cgataaattt ggcgtaccgt ggatgattaa
    26161 tgtcgtcaaa caacaaccaa cgcaataacc cgccgggagg cccgccctcc cgcactgtca
    26221 tcgaattccc gttaactctt catctgttag tcacttttaa ttaaccaaat cgtcacaata
    26281 atccgccacg atggagccac ttttttaggg aggctgcatc atgcaaacga ttatccgtgt
    26341 cgagaagctc gccaaaacct tcaatcagca tcaagcgctg catgcggttg atctgaacat
    26401 tcatcacagt gaaatggtgg ctctgcttgg gccgtcgggt tccggcaaat ccaccctttt
    26461 acgtcattta agcggtttga ttaccggcga taaatccgcc ggtagccata tcgagctact
    26521 gggccgcact gtccagcgcg aaggccgcct ggcccgcgat atccgcaaaa gccgcgccaa
    26581 caccggctac atctttcaac aattcaacct ggtgaaccgc ctgagcgtac tggagaacgt
    26641 gctgattggc gcgctcggca gcacgccgtt ctggcgcacc tgttttagct ggttcaccgg
    26701 cgagcagaaa cagcgcgcgt tacaggcgct gacccgcgtt ggcatggtgc attttgccca
    26761 tcagcgcgtt tccaccctct ccggcggcca gcagcaacgt gtggcgattg cccgtgcgct
    26821 gatgcagcag gcgaaagtga ttctggccga tgaacccatc gcctcgctgg acccggaatc
    26881 agcgcgcatc gtgatggaca ccctgcgcga catcaaccag aacgacggca tcaccgtggt
    26941 cgtcacgctg catcaggtgg attacgccct gcgctactgc gaacgcatcg tcgccctgcg
    27001 tcaggggcac gtcttctacg acggcagcag ccaactgttt gataacgaac gttttgacca
    27061 tctctaccgc agcattaacc gcatcgaaga gaacgcgaaa gctgcctgat atccccatca
    27121 ttgaggaaaa cgaatgaacg ctaagataat tgcctcgctg gccttcacca gcatgttcag
    27181 cctcagcacc ctgttaaccc cggcacacgc cgaagagcag gaaaaggcgc tgaatttcgg
    27241 cattatttca acggaatcac agcaaaacct gaaaccgcaa tggacgccgt ttttacagga
    27301 tatggagaag aaactgggcg tgaaggtcaa cgccttcttt gccccggact acgcgggcat
    27361 tatccagggg atgcgcttca ataaagtgga tattgcctgg tacggcaacc tgtcggcgat
    27421 ggaagcggtg gatcgcgcca atggccaggt cttcgcccag acggtcgcgg cggatggatc
    27481 gccgggttac tggagcgtgt tgatcgtcaa caaagacagc ccgatcaaca acctgaacga
    27541 tctgctggcg aagcggaaag atctcacctt cggcaatggc gatcctaact ccacctctgg
    27601 cttcctcgtc cccggttact acgtcttcgc taaaaacaat atctccgcca gcgacttcaa
    27661 gcgcaccgtc aacgccgggc atgaaaccaa cgcgctggcc gtcgccaaca agcaagtgga
    27721 tgttgccacc aacaacaccg aaaacctcga caagctgaaa acctccgcgc cggagaagct
    27781 taaagagctg aaggtgatct ggaaatcgcc gctgatccta ggcgatccga tcgtctggcg
    27841 caagaatctg tccgaaacta ccaaagacaa gatctacgac ttctttatga actacggaaa
    27901 aacgccggaa gagaaagcgg tgctggaacg cctgggctgg gcgccgttcc gcgcttccag
    27961 cgacctgcaa ctggtgccga ttcgccagct cgcactgttt aaagagatac aaagcgtgaa
    28021 aggcaataaa gggctgaatg agcaggacaa gctggcgaaa accaccgaga ttcaggcgca
    28081 gctggatgac ctggaccgcc tgaacaacgc gttaagcgcg atgagttcgg tgagtaaagc
    28141 ggtgcagtaa atcgtaggtc ggataagacg ccccggcgtc gcatccgaca atgtgtaggc
    28201 gttgatgccg gatgcggtgc aagcacctta tccggcctac agaccggagc caaacatgca
    28261 aaccatcacc atcgccccac ccaagcgtag ctggttctcg cttctgagct gggccgttgt
    28321 tctcgacgtg ctggtcgtct cgtggcaggg cgcggaaatg gccccgctta cgctgattaa
    28381 agacggcggc aacatggcaa cc
//
LOCUS       DAOFMX010000057        28338 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.057, whole genome shotgun sequence.
ACCESSION   DAOFMX010000057 DAOFMX010000000
VERSION     DAOFMX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.057"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="QBA20_002682"
                     /pseudo
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="QBA20_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(998..2020)
                     /locus_tag="QBA20_002683"
     CDS             complement(998..2020)
                     /locus_tag="QBA20_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6293161.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="QBA20_002684"
     CDS             complement(2034..3536)
                     /locus_tag="QBA20_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDQ6293162.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="QBA20_002685"
                     /pseudo
     CDS             complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="QBA20_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4942..5472
                     /gene="ppa"
                     /locus_tag="QBA20_002686"
     CDS             4942..5472
                     /gene="ppa"
                     /locus_tag="QBA20_002686"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDQ6293163.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="QBA20_002687"
     CDS             complement(5851..6192)
                     /locus_tag="QBA20_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDQ6293164.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="QBA20_002688"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="QBA20_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDQ6293165.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGPRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="QBA20_002689"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="QBA20_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDQ6293166.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIPRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12548
                     /gene="msrA"
                     /locus_tag="QBA20_002690"
     CDS             11910..12548
                     /gene="msrA"
                     /locus_tag="QBA20_002690"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDQ6293167.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNSYGYCGIGGIGVCLPPEA"
     gene            12871..14214
                     /locus_tag="QBA20_002691"
     CDS             12871..14214
                     /locus_tag="QBA20_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDQ6293168.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14276..14482)
                     /locus_tag="QBA20_002692"
     CDS             complement(14276..14482)
                     /locus_tag="QBA20_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDQ6293169.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14807..15361
                     /locus_tag="QBA20_002693"
     CDS             14807..15361
                     /locus_tag="QBA20_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDQ6293170.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15424..16362)
                     /locus_tag="QBA20_002694"
     CDS             complement(15424..16362)
                     /locus_tag="QBA20_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293171.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="QBA20_002695"
     CDS             complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="QBA20_002695"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDQ6293172.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17504..19447
                     /gene="cpdB"
                     /locus_tag="QBA20_002696"
     CDS             17504..19447
                     /gene="cpdB"
                     /locus_tag="QBA20_002696"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDQ6293173.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPDVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19573..19953)
                     /locus_tag="QBA20_002697"
     CDS             complement(19573..19953)
                     /locus_tag="QBA20_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDQ6293174.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20042..20902
                     /gene="qorB"
                     /locus_tag="QBA20_002698"
     CDS             20042..20902
                     /gene="qorB"
                     /locus_tag="QBA20_002698"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDQ6293175.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21011..21976
                     /locus_tag="QBA20_002699"
     CDS             21011..21976
                     /locus_tag="QBA20_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDQ6293176.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22084..22746
                     /gene="ytfE"
                     /locus_tag="QBA20_002700"
     CDS             22084..22746
                     /gene="ytfE"
                     /locus_tag="QBA20_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDQ6293177.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="QBA20_002701"
                     /gene_synonym="dagA"
     CDS             complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="QBA20_002701"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDQ6293178.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="QBA20_002702"
     CDS             complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="QBA20_002702"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDQ6293179.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25350..25895
                     /locus_tag="QBA20_002703"
     CDS             25350..25895
                     /locus_tag="QBA20_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HDQ6293180.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25879..26526)
                     /locus_tag="QBA20_002704"
     CDS             complement(25879..26526)
                     /locus_tag="QBA20_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDQ6293181.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26738..27529
                     /locus_tag="QBA20_002705"
     CDS             26738..27529
                     /locus_tag="QBA20_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HDQ6293182.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27539..>28338
                     /locus_tag="QBA20_002706"
     CDS             27539..>28338
                     /locus_tag="QBA20_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDQ6293183.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6953 a         7541 c         6990 g         6854 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt atgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     4621 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     4681 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     4741 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     4801 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     4861 cgaaaacaag caaagacatt cggcgcgagt tggctataat acttggcact tgtttgccac
     4921 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     4981 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
     5041 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
     5101 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
     5161 gtcccgactc cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttgccgtt
     5221 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
     5281 ctgagcaaag aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg
     5341 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
     5401 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
     5461 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg
     5521 tttttttatg cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc
     5581 aaggcttgaa cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg
     5641 ctggaagtta gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct
     5701 gcacatttgt gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg
     5761 agagaaaagc aaaagatgga aaagttacaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggaggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcattac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcgggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc cacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccaag tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgacccccag atgcagatta cccgctttta ccgccacgcc tagctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggggaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccgacgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgtgct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtctccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccag cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ccaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttaaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgctagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa ctcatatggt tactgtggaa ttggcggaat tggcgtctgt ctgccgccgg
    12541 aagcatagcg ttacgggtac aaatgtagtt tgttgataaa gtgctctttg tctatgcctg
    12601 atgcggctta aacgacttac caggcctaca aaatcgccca aattcaatat attgcaagga
    12661 ctgcgtaggc ctgatcggca tagcgcatca ggcagtattg catttatcag cagtctgaat
    12721 tttaaccctc tggcgacttt acagtacctt acgctatact agccactgaa aatgccggtt
    12781 cactttcttc gaatcggctt tcaatgtgta tttcacacaa attaatcaac tttcccttcc
    12841 gagggtctgg cctgaaaggt cggataagat atgttaaaca gtattttagt catactctgc
    12901 ttgatcgctg taagtgcgtt cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa
    12961 atcaaactta aactgctggc tgatgaaggc aatataaatg cccaacgcgt tctgaatatg
    13021 caggaaaatc ccggcatgtt ctttaccgtg gtccaaatcg gtctgaacgc agtggcgatt
    13081 ctcggcggta tcgtcggtga tgcggcattt tctccagctt ttcacagcct gttctcccgc
    13141 tatatgtcgg cagagctctc tgagcaactg agctttattc tctctttctc gttagtgact
    13201 ggcatgttta tcctgtttgc ggatttaacc ccgaaacgca tcggtatgat tgcgccagaa
    13261 gcggtggctt tgcgtatcat caacccgatg cgcttctgcc tgtacgtttg caccccgctg
    13321 gtgtggttct tcaacggcct ggcgaacatg atcttccgta ttttcaaact gccaatggta
    13381 cgtaaagatg acatcacttc tgatgacatc tacgcggtag tggaagccgg tgcgctggcg
    13441 ggcgtgttac gtaaacagga acacgagctg attgaaaacg tctttgagct ggaatcccgt
    13501 accgttccgt cttcaatgac accgcgtgaa aacgtgattt ggtttgatct ccacgaagat
    13561 gagcaaagcc tgaagaataa ggtggcggaa catccgcact ctaagttcct cgtctgtaat
    13621 gaagatattg accacatcat cggttatgtc gattctaaag acctgctgaa ccgcgtgctg
    13681 gctaaccaaa gcctggcact gaacagcggc gtacaaattc gcaacacgct gattgtgccg
    13741 gatacgttaa ccctttcaga ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg
    13801 gtgatcatga acgagtacgc gctggtggtg gggatcatca ccctcaacga cgtgatgacc
    13861 acgctgatgg gcgatctggt cggtcagggg ctggaagagc agattgtcgc ccgtgatgag
    13921 aactcatggc tgattgacgg cggcacccca attgacgacg tcatgcgcgt gctggatatt
    13981 gacgagttcc cgcagtcggg caactacgaa accatcggcg gctttatgat gtttatgctg
    14041 cgtaagatcc cgaaacgcac cgattcggtg aaattcgccg gctacaaatt tgaagtggtg
    14101 gatatcgata actaccgcat cgaccagctg ctggtgaccc ggatcgacag caaggccacc
    14161 gccctttcgc caaaactgcc tgacgctaaa gataaagaag aaagcgtcgc gtaaccccca
    14221 gaaacatcaa cggctcctga atcaggagcc gtcttattac tgcatagcac tttggttaag
    14281 ccatctctgt ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca
    14341 gcttatcctt cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaatgt
    14401 ccttgatgta taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt
    14461 tgtaccgttg gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca
    14521 caatcggccc aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg
    14581 gcaatgtttc caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc
    14641 tttttaccga agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg
    14701 accgcaaact ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc
    14761 agacccaagt ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa
    14821 gattctggca ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaat
    14881 cggtcagcga gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga
    14941 tcagtttagc tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca
    15001 acatattgct ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa
    15061 atcagcgaag ttaccgcacg atcgttacca gaccaccacc attgttaaca ccgacgacgc
    15121 gattccgggt tccggcatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc
    15181 ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga
    15241 agaaagttcc gctgtagtgg tgctggataa agacggtcgc gtgcaatggg ccaaagacgg
    15301 ggcgctcaca ccggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata
    15361 atggcaataa cataaattct ggaaaaataa ttttccccta tcgttatcta tgggataggg
    15421 gaattaagtc tctgagttct tacaccgaac taatactggt aacgctagtt gctctttttt
    15481 aaacgcctca atggtcgatt tatatttctc gcaaatcttt tctttggtaa tattactatc
    15541 tgcggccaga ttcgcactta ctatcgcatc cacttgttcg gctaattcga cttgttggca
    15601 acctgcatta accagaaaat tataaataag agagtaatct tcatcctcca aaacaacaga
    15661 ggaaccatta cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaaagattc
    15721 tatgactatg gcaaaattat catcaattat tttttcgtaa atataccatc catcgcattc
    15781 tttaattcca ccgcctggta tttggagttc aaatttaccc cgttttaatt tgcttactac
    15841 atcctccaat aaggcataac cgaaagatac gtcaccaaac gcctggcgaa tataaccttt
    15901 aatccttttt ggtatgtcag actgtttttt ctgcaaaata tcacaggcat acaatcttct
    15961 gttttgttct aacggaaact cgcggttttt gaaatccatg aatgagttat actcatttat
    16021 tgttatggga atttcttcgt attcaaaccc actgcctcgc aatgttattg tatattcagc
    16081 accgttatct ttaaggctga aactgataag aaaattatcc atggcattaa cagtttcatt
    16141 ttttgatgaa agcacttgat aactattttt atcaatctgt gttgcgcaac gaaaggactc
    16201 ccgcaaagtt aacagactat cataattcga aacccaatct tttttcttaa atgcatgctg
    16261 aatattatta aaaaaatcgt ttgaatgtgt ttttgtatgc gatgacttta caaataactc
    16321 caccgcacca ataaaattat tgtgtctgat cctgggtaac atatggctac cttattttat
    16381 ttggttattt tatgtcacca ttaaatatct cattatagaa gtaaactatc aaaaaacgct
    16441 cattattaaa atgtaactta ctagcaattg aatttttgca ctttaaatac aatctctttt
    16501 tatttataac tttttaggtt gggataaatc aggatggcag aatcaggaaa tacactgttt
    16561 ctgccatctg aatttagtaa atggacactc tgaaccccgg attcaggaac gactcgcgcg
    16621 gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca gctgcagcca
    16681 cagcatgtcc ggcggcggtg tcccaaatat tcgttggtcc gaagcgcggg tacagctgcg
    16741 cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt
    16801 cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca
    16861 ccagcggcgg gcgcgcatcg cggacctgaa tcagcttgcg cacaccgcac tcttctttcc
    16921 aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta
    16981 aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa
    17041 taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga
    17101 cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgcc agcgtacgta
    17161 aaccgtccat gataacggtg tgagcggcaa tatccgctgc cgttaccgga gaattgtccg
    17221 ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg
    17281 cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt
    17341 gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc
    17401 atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa ggcgtttatt
    17461 ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc
    17521 aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat
    17581 catggaaacc actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc
    17641 cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt
    17701 gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta
    17761 catgtcggcg aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac
    17821 gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct
    17881 gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag
    17941 aaccaaacag ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga
    18001 cggaaaaaaa cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg
    18061 ctgggataaa gctaatttat ccggaaaagt gacggtgaat gatattaccg aaaccgtgcg
    18121 caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc
    18181 cgggctgtct gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga
    18241 aattccggac gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga
    18301 ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt
    18361 aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag
    18421 cggtaaatgg caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa
    18481 taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga
    18541 tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta
    18601 tctggcgctg gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta
    18661 tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc
    18721 cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa
    18781 aggccagttg accttccgta atgccgccga tctttatctc taccccaata cgctgattgt
    18841 agtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa
    18901 ccagattgct cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac
    18961 ttataacttt gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg
    19021 ctatgacggc gagtgccaga tgattaatgc caatgcagag cggattaaga acctgacctt
    19081 taatggcaag ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc
    19141 ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga
    19201 gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca
    19261 tccggcggca gataacaact ggcgtttagc accgatagct gccgataaga aactggatat
    19321 ccgtttcgaa acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta
    19381 tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag
    19441 taagtaaaac acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat
    19501 gcgatgctgc cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag
    19561 cgcatccggc agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa
    19621 ctcaatccag tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag
    19681 gctatattcc acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg
    19741 ttctaacgcc tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg
    19801 caggtcgcta aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg
    19861 gctggtgacg tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc
    19921 tttcagttgt tgtgacaggc taacctgact catttcatac ttaccttttt gcacgtactt
    19981 actaaaagta agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc
    20041 catgatcgct attactggtg ccactggcca acttggtcac tatattattg aatccttgat
    20101 gaaaacggtt cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc
    20161 cctggcagca caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac
    20221 atctgcactt cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg
    20281 tgccccgcag catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc
    20341 ttataccagc ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga
    20401 gacggagaaa atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta
    20461 cagcgaaaac tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc
    20521 ggcgggcgat ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcacg
    20581 cgtgattagc gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg
    20641 gacgttgaca cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca
    20701 aaatctgagc gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact
    20761 ggcggatatg ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag
    20821 caaaacgctt agcaaattga ttggctgccc aacgacaacg ttagccgaaa gcgtaagcca
    20881 tctttttaat gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa
    20941 tgacgggatg ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg
    21001 gatgccagta atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct
    21061 tatttttgtc gggccgttga tcgtgccgga atacccggcg atgttgcagt cgatggggcg
    21121 ttatctggcg ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg
    21181 tcagttggcg cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat
    21241 ttattacttc tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat
    21301 tatcggcacc ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga
    21361 cggcaaactc gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc
    21421 gagtgtgaat attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac
    21481 ctctggcatc gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa
    21541 cgcccgctgg ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggcgcaggc
    21601 gctggtcacg ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat
    21661 acaaacgccg gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat
    21721 ggttgcgata gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca
    21781 gcgattaccg acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct
    21841 gtacaccttt ttactccgcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct
    21901 gttagtgatt ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc
    21961 tgtctcagaa agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta
    22021 tagcttttgc cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca
    22081 gctatggctt atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg
    22141 gctctgtttc gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc
    22201 gcggcggcac gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct
    22261 gaacaaccga ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc
    22321 atcgtgcgct accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact
    22381 aaagtcgagc gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg
    22441 accatgctgc atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg
    22501 atgatcaaac aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag
    22561 cacgatgaag cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg
    22621 ccgccagaag cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat
    22681 gacctgatgg atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt
    22741 gagtgatcta aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc
    22801 agttcagcag cccgcttctt accaataaac agccagccca accccagcgc gataaaccac
    22861 agcggggtaa ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg
    22921 aacacaaaga acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc
    22981 gacttttcat gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc
    23041 gtccagacga acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc
    23101 acgctaggat tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc
    23161 aaccctttcg cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct
    23221 tcctgcgcca gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac
    23281 gccgcagagg tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac
    23341 acaaacagct caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac
    23401 ataatcacaa tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc
    23461 gcgcgtggca gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca
    23521 atccccacga aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc
    23581 gggaaccagc cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac
    23641 tgaaagtgca tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt
    23701 ttgatcatcg caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc
    23761 gtcagcagca gcactatcac cgccagcgag gcgacccagt cggagagatc ggggaaccag
    23821 aactgagcat aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac
    23881 cagtaagtcc agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca
    23941 ctaaaagatt tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa
    24001 aagagcataa aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc
    24061 gttttgccgg accccataaa caacccagta ccaatggcac ctccaatggc aataagctga
    24121 acatgtcggt ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca
    24181 acgactttta cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct
    24241 ctacggcctt tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg
    24301 taacaatacg cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg
    24361 agtgtcatat cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt
    24421 tgcaacacaa aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc
    24481 ctctggttcg aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa
    24541 caccagggtg ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc
    24601 ctgcgggata gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca
    24661 gccagggatc acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc
    24721 aaacacggtg ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc
    24781 cggaattgcg ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt
    24841 cacaccttct tttttggcgt tttcttccag gtatttcacg ccttcagcag ccatcgcctg
    24901 gaaacgctga cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac
    24961 cacatcaacc ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag
    25021 tgcttctggc agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc
    25081 aatgccgtag cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc
    25141 tttcttaggg tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt
    25201 gtcgcaggaa tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat
    25261 ataataaagg cgggtttacc acggacatca tagcgttagc ttattcatag gctatgattg
    25321 aggaacaaga cgtggagcag gaggaaacca tgcccgggcg ctttgaacta aaaccaaccc
    25381 tggagaaagt ctggcacgcg ccggacaatt ttcgcttcat ggacccgctg ccgccgatgc
    25441 atcgtcgggg gatcattatt gccgccattg tattggtggt cggatttctg cttccatctg
    25501 atgatacgcc caacgcgtcg gtggtgacgc gtgaagcgca gttagatatt cagtcgcaat
    25561 cacaaccgcc gacagaagaa cagccgcaaa ctacccaaac gcaaccattc cagccagata
    25621 gcggaattga taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc
    25681 tgttccgcga ccacggactg cccgctaccg atgtctatgc aatggcgcag gtagaaggcg
    25741 cgggtaagcc gctgagtaat ttgcaaaatg ggcagatggt gaaaatccgc cagaacgcca
    25801 gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc ctgtttaccc
    25861 gccagccgga tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca aaagccagct
    25921 aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctgggggg aaaccatact
    25981 gatatgataa actaggccaa gcgtatgctg gttgatcaaa taatatcccc caagcgcagc
    26041 aatattaata ttcacctgca atgggtcgat acctggctta aaaatattgt atttcttccc
    26101 ttcatccaat aaggattcca ttaactgcaa gtgagcatgg ttaatttcta agagacgctg
    26161 tgattttgca taatgcacac ctttactttg attttcacta tgcacaattt ttaaaaacca
    26221 aggattagca atataataat cccatgtaaa atcaataatt ttctcaatgg ctttttgtgg
    26281 cggaagatct gagaaatcaa gggccttttc tttttgtcga atatcgttcc aggtatattc
    26341 cagcacttcg ataaataaat tttctttatt accaaagtgg tgataaaccg tctgtttact
    26401 acaccctgct gcctttacaa tattatctac tctggcccct tcataaccat attcggcaaa
    26461 ttcattaacc gcacatagca gcaatttttc cttcaggctc tgtacgttgc tggtatcaat
    26521 atacataacg cttccccacg acctcttcgt ggtgactatc ataacctgat ttgatttcag
    26581 cttcagaaaa aagggcaaaa acttgtactt tcatcaggtt atcaaatgtc ctgtgatcgc
    26641 catcgtatat caaccaacta gatggttgat ctttttatcc tccagtcgca agctaatcat
    26701 caccaaggaa acaaaattca aaaaagagag ctattgtatg aaatccactc gtcctgttgc
    26761 cgtgattacc ggagccactc gcggtattgg aaagggctgt gcgcaggagc ttgctcgtgg
    26821 cgggtttaac ctgctgatta acgatcgtcc cgatgccgac agcgtagaaa aactgcatat
    26881 cacacaacaa gaatgtttgg cggaaggcgt ggaagtgatt tgcttccctg ctgatgtcgg
    26941 tgatctatct ctgcatgagg agatgctcga cgcggcgcaa aaccagtggg ggcggctgga
    27001 ctgtctgctc aataacgcgg ggatttcagt gaaaaaacgc ggtgacctcc tcgacctcga
    27061 accagacagc ttcgatcaaa acattgccat taacacccgc gcgccgttct tccttgctca
    27121 ggcattcagt aagcgcttac tggcacagcc gaaaccggaa gcggagctgc cgcaccgctc
    27181 gattattttc gtcagttcca tcaacgccat catgctggcg atgaatcgcg gcgaatacac
    27241 cattgccaaa accgccgttt ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga
    27301 acaaattggc gtttatgaag tgcgccctgg tctcatcaaa actgatatga ctattccggc
    27361 aacggcttat tacgacgaac tgattgccaa aggtctggtg ccgtggggac gttggggtta
    27421 tccggctgat attgcttcca ccgtccgcgc gatggcggaa ggtaaactga tttacacctg
    27481 tggtcaggcg gtcgcaatcg acggcggctt aagtatgccg cgcttctgag gctgaatcat
    27541 gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcgacac tgggctataa
    27601 aacgccgcta cccgccatta ttgatgcctg cgccgcacgc ggtattggcg caattgcccc
    27661 ctggcgcagg gagttgcagg gtgaagatct ccagcaaatc acccgccagc ttgccgccag
    27721 taacatgagc gtttccggtt tgtgccgtag cacttactac accgcgccga cactggcgga
    27781 acgcaaactg gcgattgatg ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc
    27841 cgcctgttat atgcaggtgg ttggcggtct gccaacgggc actaaagatc tatatgaagc
    27901 gcgagaacaa gtgaaacaag ggattcgcca gttgctgcca cattcgaaag atgtcggcgt
    27961 gccaattgcg ctggagccac tgcacccgat gacagctgcc gatcgctctt gcctgtgcac
    28021 gttacgtcag gcgctggact ggtgtgatga actcgatccc gatggtgagt ttggcctcgg
    28081 cgtggcggtg gatgtttatc acgtctggtg ggatccggat ctcgccagtc agatcctgcg
    28141 tgccggaaaa cgcatcctcg cgtttcatgt ttccgactgg ttaatgccga ccactgatct
    28201 ggtcaatgac cgtggtatgc cgggagatgg cgtcatcaat attccgtcaa ttcgtcggct
    28261 ggttgaaaac gccggattta atggtgctat tgaactggaa atattctcgc cttactggtg
    28321 gcagaaagat attaacag
//
LOCUS       DAOFMX010000058        27601 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.058, whole genome shotgun sequence.
ACCESSION   DAOFMX010000058 DAOFMX010000000
VERSION     DAOFMX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.058"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="QBA20_002707"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="QBA20_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDQ6293184.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="QBA20_002708"
     CDS             complement(1614..1937)
                     /locus_tag="QBA20_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDQ6293185.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="QBA20_002709"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="QBA20_002709"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDQ6293186.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="QBA20_002710"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="QBA20_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDQ6293187.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="QBA20_002711"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="QBA20_002711"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDQ6293188.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="QBA20_002712"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="QBA20_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDQ6293189.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="QBA20_002713"
     CDS             7537..8574
                     /locus_tag="QBA20_002713"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDQ6293190.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="QBA20_002714"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="QBA20_002714"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDQ6293191.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="QBA20_002715"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="QBA20_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDQ6293192.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="QBA20_002716"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="QBA20_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDQ6293193.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="QBA20_002717"
     CDS             10745..11626
                     /locus_tag="QBA20_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6293194.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="QBA20_002718"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="QBA20_002718"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDQ6293195.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTTLLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="QBA20_002719"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="QBA20_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDQ6293196.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="QBA20_002720"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="QBA20_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="QBA20_002721"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="QBA20_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDQ6293197.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="QBA20_002722"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="QBA20_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDQ6293198.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="QBA20_002723"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="QBA20_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDQ6293199.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="QBA20_002724"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="QBA20_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDQ6293200.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="QBA20_002725"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="QBA20_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDQ6293201.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="QBA20_002726"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="QBA20_002726"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDQ6293202.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="QBA20_002727"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="QBA20_002727"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDQ6293203.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="QBA20_002728"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="QBA20_002728"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDQ6293204.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="QBA20_002729"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="QBA20_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDQ6293205.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="QBA20_002730"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="QBA20_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDQ6293206.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="QBA20_002731"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="QBA20_002731"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDQ6293207.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="QBA20_002732"
     CDS             24893..25273
                     /locus_tag="QBA20_002732"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDQ6293208.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="QBA20_002733"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="QBA20_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDQ6293209.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="QBA20_002734"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="QBA20_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDQ6293210.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="QBA20_002735"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="QBA20_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6809 a         6864 c         7235 g         6693 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tctagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggtgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgtggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaactct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgatggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagcaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAOFMX010000059        26607 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.059, whole genome shotgun sequence.
ACCESSION   DAOFMX010000059 DAOFMX010000000
VERSION     DAOFMX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.059"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(11..151)
                     /locus_tag="QBA20_002736"
     CDS             complement(11..151)
                     /locus_tag="QBA20_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDQ6293211.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            393..686
                     /locus_tag="QBA20_002737"
     CDS             393..686
                     /locus_tag="QBA20_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDQ6293212.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            758..1417
                     /locus_tag="QBA20_002738"
     CDS             758..1417
                     /locus_tag="QBA20_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDQ6293213.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1509..1955
                     /locus_tag="QBA20_002739"
     CDS             1509..1955
                     /locus_tag="QBA20_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDQ6293214.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2162..3062)
                     /gene="hlyE"
                     /locus_tag="QBA20_002740"
                     /pseudo
     CDS             complement(2162..3062)
                     /gene="hlyE"
                     /locus_tag="QBA20_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3435..3854
                     /locus_tag="QBA20_002741"
     CDS             3435..3854
                     /locus_tag="QBA20_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDQ6293215.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3854..5122
                     /gene="umuC"
                     /locus_tag="QBA20_002742"
     CDS             3854..5122
                     /gene="umuC"
                     /locus_tag="QBA20_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDQ6293216.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5168..5698)
                     /gene="dsbB"
                     /locus_tag="QBA20_002743"
     CDS             complement(5168..5698)
                     /gene="dsbB"
                     /locus_tag="QBA20_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDQ6293217.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5844..7385)
                     /gene="nhaB"
                     /locus_tag="QBA20_002744"
     CDS             complement(5844..7385)
                     /gene="nhaB"
                     /locus_tag="QBA20_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDQ6293218.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7606..8325
                     /gene="fadR"
                     /locus_tag="QBA20_002745"
     CDS             7606..8325
                     /gene="fadR"
                     /locus_tag="QBA20_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDQ6293219.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8377..9909)
                     /locus_tag="QBA20_002746"
     CDS             complement(8377..9909)
                     /locus_tag="QBA20_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDQ6293220.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDFR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10239..11537
                     /gene="dadA"
                     /locus_tag="QBA20_002747"
     CDS             10239..11537
                     /gene="dadA"
                     /locus_tag="QBA20_002747"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDQ6293221.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11547..12617
                     /gene="dadX"
                     /locus_tag="QBA20_002748"
     CDS             11547..12617
                     /gene="dadX"
                     /locus_tag="QBA20_002748"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDQ6293222.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12785..14199)
                     /locus_tag="QBA20_002749"
                     /pseudo
     CDS             complement(12785..14199)
                     /locus_tag="QBA20_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14810..16546)
                     /locus_tag="QBA20_002750"
     CDS             complement(14810..16546)
                     /locus_tag="QBA20_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDQ6293223.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16641..17555)
                     /gene="ldcA"
                     /locus_tag="QBA20_002751"
     CDS             complement(16641..17555)
                     /gene="ldcA"
                     /locus_tag="QBA20_002751"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDQ6293224.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17655..18266
                     /gene="emtA"
                     /locus_tag="QBA20_002752"
     CDS             17655..18266
                     /gene="emtA"
                     /locus_tag="QBA20_002752"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDQ6293225.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18268..19002)
                     /locus_tag="QBA20_002753"
     CDS             complement(18268..19002)
                     /locus_tag="QBA20_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDQ6293226.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKTAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19203..19457
                     /locus_tag="QBA20_002754"
     CDS             19203..19457
                     /locus_tag="QBA20_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDQ6293227.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19507..20967)
                     /locus_tag="QBA20_002755"
     CDS             complement(19507..20967)
                     /locus_tag="QBA20_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDQ6293228.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKATGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20904..21461)
                     /locus_tag="QBA20_002756"
     CDS             complement(20904..21461)
                     /locus_tag="QBA20_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HDQ6293229.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21487..22341)
                     /locus_tag="QBA20_002757"
     CDS             complement(21487..22341)
                     /locus_tag="QBA20_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HDQ6293230.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22341..23150)
                     /locus_tag="QBA20_002758"
     CDS             complement(22341..23150)
                     /locus_tag="QBA20_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDQ6293231.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23160..23918)
                     /locus_tag="QBA20_002759"
     CDS             complement(23160..23918)
                     /locus_tag="QBA20_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6293232.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23915..24895)
                     /locus_tag="QBA20_002760"
     CDS             complement(23915..24895)
                     /locus_tag="QBA20_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDQ6293233.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24895..25917)
                     /locus_tag="QBA20_002761"
     CDS             complement(24895..25917)
                     /locus_tag="QBA20_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HDQ6293234.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6686 a         6483 c         6866 g         6572 t
ORIGIN      
        1 agaacctcgc ttaggcctgt gtccatatta cgtgggtagg atcactatgg cctttgctga
       61 actcaacatt gacattctta cccgcagcaa agctcgtgga ggttagcagt gcaagcagca
      121 atacagcctt actgatgctc ttgattttca ttggtagtct cctgttcaga atgagtacga
      181 tgaactgtta taatataaca atcccttacg ggagaattcc agtggctttt ctgcgttcgg
      241 taaaatcccg gcgcggcatt ttttgccggg gggattactt ataaaggggc atggactcaa
      301 caaaccgcag aaatctacag ctggctggct tgtggcgcgg gtttcattga tgttagctta
      361 tgccaaaacc tggcatctta aagagtaaaa gtatgttttg tgtgatttat cgaagcagca
      421 agcgtgacca gacctattta tatgtcgaaa aaaaagacga tttttcgcgt gttcctgagg
      481 aactgatgaa aggttttggt cagcctcagt tagcgatgat tctgccgttg gatgggcgga
      541 agaaactggt caatgctgat attgaaaagg ttaaactggc attaaccgag caaggttact
      601 atttgcagtt accgccacca cccgaagatt tgctgaagca acatctttcc gtcatggggc
      661 agaaaacaga cgacactaac aaataaccga tatccggcgg tggcattatc tttgtcggcg
      721 cgggttttca tatccacgat aaggtgaggg gaacgttatg tatcaacatc acaactggca
      781 aggtgcgctg ctggattatc cggtgagtaa agtagtctgt gttggcagta actatgccaa
      841 acatattaaa gagatgggca gcgcagtgcc cgaagagcca gtgctgttta ttaaaccaga
      901 aacggcactg tgcgatctgc ggcagccatt ggcgatccca tccgatttcg gttcagttca
      961 tcatgaagtc gaactggcgg tgttgattgg cgcgacgctg cgtcaggcaa cggaagagca
     1021 tgtccgcaaa gccattgccg gttacggcgt ggcgcttgat ctgacattgc gtgatgttca
     1081 gggaaaaatg aagaaagccg ggcagccgtg ggaaaaggct aaagcgtttg ataactcttg
     1141 tccgctttcc gggtttatcc ccgcggcgga atttaccggc gatccacaaa atacaacgct
     1201 gggcctgagc gtaaacggcg aaaaacgcca gcaaggtacg actgcggaca tgatccataa
     1261 aatcgttccg ttgatcgctt atatgagcaa gttttttacc ctcaaggccg gtgacgttgt
     1321 gttgacaggc acgcctgatg gcgtcggccc gttgcaaagc ggtgatgagc tgacagtcac
     1381 tttcgatggg cattctttga caactcgcgt tttgtaatac tttttgccgc cagaaagcgg
     1441 cggcaaaact tgcatcgctg tgccagactg gttataaggt gcgttttaac gtaatggcgg
     1501 aacacctgat gagcgatgta cctttctggc aaagtaaaac cctggacgaa atgagcgatg
     1561 cggaatggga gtcgttgtgt gatggttgcg gtcagtgttg cttgcataaa ctgatggatg
     1621 aagacaccga cgaaatctac ttcactaacg tcgcctgtcg ccagctcaat attaaaacct
     1681 gtcaatgtcg gaactacgaa cgtcgtttcg agtttgaacc cgactgcatt aaattaaccc
     1741 gtgaaaatct gccaacattc gaatggctgc caatgacctg cgcttatcgg ttgctggcgg
     1801 aaggtaaaga tttacctgcg tggcatccgc tacttactgg ttcgaaagcg gcaatgcatg
     1861 gtgaacgtat ctctgtgcgt catatcgcag tgaaagaatc agaagtgatt gactggcagg
     1921 atcatatctt aaataaacct gactgggcgc agtgataaat ttaatagatg ctgatggggg
     1981 ctttcttaat atacgtctgg atgctcgcca tttcgtagcc ctgtttttga aagcgctctg
     2041 tcataatgag agttacccga taccttatta tcaacaggta actctcaatc tttaaaaaac
     2101 gtctgtgttt ttaaatgtga taaaccttat accttgagta catggagaga ataatcgctt
     2161 atcagacttc aggtacttca aagagtgtct ttttaccgtg tcttttctga tactcattac
     2221 aggtgttaat catttttttg gccgctgctt ttagcaaaga aagcattaaa tcatcataat
     2281 caacatagaa tctggttgtt tcagtttccg tttttatctc cccgatggcg gctatttcgg
     2341 tggttaattt caatttggcg gcatcgatat ctttattcgc ttgtttaacc gtgttagaca
     2401 gggtggtaaa gaaactctgc acagatttta acttgttctt caattctgga atcagtttcc
     2461 cttcaactac gcccgcagca atagaatagg aaatgattaa accaaatgga ccggcgacga
     2521 catcggctgc ggcaccggca tacgcttcct tcctgatttt atctacctgt gactggaaat
     2581 agctgctttt ttctgaaaaa tcattggtta actggctatc taacgccagc agtttcccgg
     2641 aagcgttgtt gaaactttgt gagcttacca gcagggattt ttgcgcttca ttcagcttcg
     2701 tgatgccgtc atccagtacc ttaatgaggg gcggatgctt tcttctcatt gtactcatca
     2761 aatagcaaaa tatacgctgc gagcaattgc gtcgcaacac cacaccattc atacactgtt
     2821 tgggttgctt caaaatactt atcctggcta tccataagta aggttttaat atcgccgact
     2881 aaaacggagg ctgcctgtga atactcctgt ttaaagcgac ttaactcttt tatggtttca
     2941 tcaaaggtct gccaggggat gacctgatcg agatatttat tataaagatc taatgctcca
     3001 tctgcggttt cgattgcgtt tttaactact tctaccgttt tatctgcaac gatttcagtc
     3061 ataatcattc gcctctttaa atatataaat tgtaatgaaa ctcctgtttt acaactatta
     3121 ataaatttta cttcatctaa ttcatagtta gccgggcggg atgcgtcaat gtctttattt
     3181 ctattaatat gataaatatc aaacaatgtt taatgtcatt atggcgaatg cttctattct
     3241 atttttttgc cgggtgatat ttttcatttc tgctggatga gcgtcgtcgc cagaaggcca
     3301 cgtgagcaca ggataagaga acgaaaaatc agcagtctat gcagcgacaa atattgatag
     3361 cctgaatcag tattgatctg ctggcaagaa cagaccactg tatataaaaa cagtataact
     3421 tcaggcagat tattatgttg tttatcaagc ctgcggatct ccgcgaaatt gtgacttttc
     3481 cgctatttag cgatcttgtt cagtgtggct ttccttcacc ggcagcagat tacgttgaac
     3541 agcgcatcga tctgaatcaa ctgttgatcc agcatcccag cgcgacttac ttcgtcaaag
     3601 caagtggtga ttctatgatt gatggtggaa ttagtgacgg tgatttactg attgtcgata
     3661 gcgctattac cgccagccat ggtgatattg tcatcgctgc tgttgacggc gagtttacgg
     3721 tgaaaaaatt gcaactacgc ccgacggtac agcttattcc catgaacagt gcgtactcgc
     3781 ccattaccat cagtagcgaa gatacgctgg atgtctttgg tgtggtgatc cacgtcgtta
     3841 aggcgatgcg ctgatgtttg ccctctgtga tgtaaacgcg ttttatgcca gctgtgagac
     3901 ggtgtttcgc cctgatttat ggggtaaacc ggtggttgtg ctatcgaata atgacggttg
     3961 cgttatcgcc cgaaacgctg aggcaaaggc gctgggcgtt aaaatgggcg atccctggtt
     4021 caaacaaaaa gatctgtttc gtcgctgtgg tgtggtttgc tttagcagca attatgagct
     4081 ttacgcagac atgagcaatc gggtgatgtc gacgctggaa gaactatcgc cccgcgtcga
     4141 gatttacagt attgatgaag cattctgcga tctgacaggt gtgcgtaatt gtcgcgatct
     4201 gactgatttt ggcagagaaa ttcgcgcaac ggtgctacaa cgtacccatc ttactgttgg
     4261 tgtggggatc gcccagacca aaacgttggc taagcttgcc aatcatgcgg caaaaaaatg
     4321 gcagcggcag acgggtgggg tggtggattt atcaaatctg gaacgccagc gtaaattaat
     4381 gtctgctctc cccgtggatg acgtctgggg gattggacgg cggatcagca aaaaactgga
     4441 cgcgatgggg atcaaaaccg ttctcgattt ggcggataca gatatccggt ttatccgtaa
     4501 acattttaat gtcatgctcg aaagaacggt gcgtgaactg cgcggcgaac cctgtttgca
     4561 actggaagag tttgcaccga cgaagcagga aattatctgt tcccgctcgt ttggtgaacg
     4621 catcacggat tatacgtcga tgcggcaggc catttgtagt tacgctgccc gggcggcgga
     4681 aaaacttcgc agtgagcatc aatattgtcg gtttatctcc acatttatta agacgtcacc
     4741 atttgcgctc aatgaacctt attacggcaa tagcgcgtcg gtaaaactgc tgacgcccac
     4801 tcaggacagc agggatatca ttaacgctgc tacgcgatct ctggatgcca tctggcaagc
     4861 gggccatcgc taccaaaaag cgggcgtgat gctgggggat ttcttcagtc agggagtcgc
     4921 gcagctcaat ttattcgatg acaacgcacc gcgccccggg agtgagcaat tgatggcggt
     4981 aatggatact ctgaatgcta aagagggcag aggaacactc tattttgccg ggcaggggat
     5041 ccaacaacaa tggcagatga agcgagctat gctttcacca cgttatacaa cgcgaagttc
     5101 tgatttactg cgggtcaaat aaatatagcg gcaggaaaaa agcgctcccg caggagcgcc
     5161 gaatggatta gcgaccgaac agatcacgtt ttttcgcttt aaacggctgg gaaatcacca
     5221 ccagcaccgc gacaatcagg taagcgataa aaataccgag cagccactgc ggcatttcca
     5281 tacctaaaaa atcccactga cgctcggcgc aatcgccaga ggcgacaaac acttgcggca
     5341 cccatttatc cagcggcagc cattccggga aacgaaccat aaaatcacag gtggcaaatg
     5401 gcgaaggata gagctgaagc atggtgtgct cgtaagttaa ctgcacaccg cggaacgcac
     5461 tatacaacca gataaccatc gctacataac gcagcggagt tttcggggcg atcgcgccaa
     5521 tcagcgcagc acccagaacg ccgaataacg cgcagcgttc ataaatacag agcacgcaag
     5581 gtttcagtaa catcacatgc tggaaccaca gcgccgtcag ttccagtgcc agagcagtaa
     5641 acgccatcaa cagccacgcg ccccggcctt gtgaacattg gttcaaaaat cgcaacataa
     5701 tcatttccct gcaatatgca tagagtgcgc agtttaaacc aattcattcg ctgcgccacc
     5761 aggaggcagc aaaaatagga cgcaattgga caattatcgg gcgaaaaggc aaaccgggca
     5821 tttatatgcc cggtaagttg ttatcaaagc gttgctatcc agcccatttg cataaaccat
     5881 tcggttacag gggcaagcgt aaactcgacg cagagcaagc cgacgagtgt cagtacgagg
     5941 gtgtaaggca gagccatcca caccatgcgg ccataagaga ggcgaatcaa tggcgcgagt
     6001 gcagaggtca gcaggaacag gaacgcagcc tgaccgttcg gcgtagcgac agagggcaga
     6061 ttggtaccgg tattaatggc gaccgccagc agctcgtatt gcttcaacgt gatagcgcca
     6121 ctttccattg ccgctttcgc ttcgttgata taaatcgtcc ccacgaagac gttatccgaa
     6181 atggatgaca gcagaccgtt gaaaatatag aacagcgaca gctgagcatg ttcagatgcc
     6241 tgcaacacaa actgaataat cggcgaaaac agttgttggt cgataatcac cgcgactacc
     6301 gagaaaaaca ccgtcaacag tgcggtgaat ggcagagatt cggtgaaggc tttaccgata
     6361 gcatgttcat cggtgacacc ggtcaatgat gttgccagaa taatgactga caaaccaatc
     6421 aagccaactt ccgccagatg caacgccagc gcagtcacca gccagacgcc aataatcgcc
     6481 tggacaatca gacggatttt atcctgacgg gtacgctggt ggcggctttg atcgtcaaac
     6541 tgttgcaaca cttcgcggac tttctccggc agcgtttcac cgtagccaaa ccaacgcagc
     6601 ttctctacca gcaggcaggt taacaggcca caaatcagaa ccggaacggt caccggcgac
     6661 atgcgcagga agaaatcgcc aaaatgccag ccagccgctt tagcgatgat caggttctgt
     6721 ggttcgccca ccatggtcat tacgccgcct aatgcggtgc cgacaccggc atgcatcatc
     6781 aggctacgca gaaagccacg gaactgttcc agaaccacgt tgtaatgctt gtcgatatga
     6841 ctatcgtctt gcaggtcggt gtcttcggta cgggaagagg ctacgcgatg ataaatacca
     6901 taaaaaccga ctgcaacgct gatcaccacc gccacgacgg ttaaggcatc gaggaacgcg
     6961 gagaggaacg ctgccgccac gcaaaaagag agcgacagca gcattttgga gcgaatgctt
     7021 aacagcaaac gggtaaatat gaacagcaac agctgtttca taaaatagat acccgccacc
     7081 ataaacatca ataacagcaa gacttcaaga tttgccgcca cctcttcacg gacgtgttcc
     7141 gcgctggtca tgccgatgaa taccgcttcg atagccaaca gaccaccggg gagtagcggg
     7201 tagcatttca gggccatcgc cagagtgaaa ataaattccg ctaccagcaa ccagcccgcg
     7261 acgaaagggc tgatgaggaa aattaacggg tttacgatta agaaaatgat gagggcgagt
     7321 ttgtaccagt cgggggactg gcccaaaaaa ttgcgccata gcgcgcggcc ccaggagatc
     7381 tccatgatgg tttcccttac cttacaaata atcaatgatg tttttatgtt taaacgcaaa
     7441 gcttaacggt caggcaggag tgaggcaagt cttgatagtc aaggggaaag agatgcggaa
     7501 aatgaagcct tgatcccttt ttcttctttt tgtctgctat cagcgtagtt agccctctgg
     7561 tatgatgagt ccaactttgt tttgctgtgt tatggaaatc tcactatggt cattaaggcg
     7621 caaagcccgg cgggtttcgc ggaagagtac attattgaaa gtatctggaa taaccgcttc
     7681 cctcccggga ctattttgcc cgcagaacgt gaactttcag aattaattgg cgtaacgcgt
     7741 actacgttac gtgaagtgtt acagcgtctg gcacgagatg gctggttgac cattcaacat
     7801 ggcaagccga cgaaggtgaa taatttctgg gaaacttccg gtttaaatat ccttgaaaca
     7861 ctggcgcgac tggatcacga aagtgtgccg cagcttattg ataatttgct gtcggtgcgt
     7921 accaatattt ccactatttt tattcgcacc gcgtttcgtc agcatcccga taaagcgcag
     7981 gaagtgctgg ctaccgctaa tgaagtggcc gatcacgccg atgcctttgc cgagctggat
     8041 tacaacatat tccgcggcct ggcgtttgct tccggcaacc cgatttacgg tctgatcctt
     8101 aacgggatga aagggctgta tacgcgcatt ggtcgtcact atttcgccaa tccggaagcg
     8161 cgcagtctgg cgctgggctt ctaccacaaa ctgtcggcgt tgtgcagtga aggcgcgcac
     8221 gatcaggtgt acgaaacagt gcgtcgctat gggcatgaga gtggcgagat ttggcaccgg
     8281 atgcagaaaa atctgccggg tgatttagcc attcaggggc gataatccct tccgtttaaa
     8341 gagcaacccc ctcaaacgag gggttttttg ttgtttttac agatttccca ttcttggcgg
     8401 gcaacgttcc agcaactcga tgctgccgtc ttcgttttgc tgttcgagca tcacatcaaa
     8461 tccccacagg cgatgcacat gcttcaggac ttctttgcgc ccccgatcca gcggtgcgcg
     8521 attatgtgga atataacgca gcgtcagcga acggtcgccg cgaaaatcca cgttccagat
     8581 ctgaatattc ggctccagat tacttaagtt atattgcgac gataaccggt tacggatctc
     8641 ccgataacct tcttcattat gaatagcgga aatctccaga taattatgcc gatcatcatc
     8701 cagcacggtg aagaagcgga aatcacgcat cactttcggt gacagaaact ggctgataaa
     8761 actctcatct ttgaaatcac gcatcgcgaa atgcagcgtt tccagccagt cggaaccggc
     8821 gatatccggg aaccagtatt tgtcttcttc cgttggcgac tgacaaatcc gtttaatatc
     8881 ctggaacatg gcgaacccga gggcatacgg gttgatgccg ctgtaccacg ggctgttata
     8941 ggggggctgg aagaccacat tggtgtggct gtgcaaaaac tccagcataa aacgttccgt
     9001 tactttccct tcatcataca gatggttaag gatggtatag tgccagaagg tcgcacagcc
     9061 ttcgttcatc acctgagtct gtttttgcgg ataaaaatac tggctcacct tacgcacaat
     9121 acgcaggatt tcacgctgcc atgattccag cagtggcgca tttttctcca taaaatagag
     9181 caggttttct tgtggttcgg acggatagcg gcgcgcttca gcaaccgttt tctcttcctc
     9241 gcgcttcggc agggtacgcc agagcatatt gacctgactt tgtagatact cttcgcgact
     9301 tttctgccgg gctttctctt cttgcagcga gattttttgc gggcgtttgt accggtccac
     9361 gccgtagttc atcagcgcgt ggcacgagtc cagaagccgt tctacttcat caacgccata
     9421 acgttcttcg cactcggtaa tatatttacg ggcaaaaatc agataatcga caatcgaact
     9481 ggcgtcggtc caactgcgga ataagcaatt gtttttaaag aaagagttat gtccatagca
     9541 ggcgtgtgcc atcaccagcg cctgcatagt aatggtgttc tcttccatca agtaagcgat
     9601 acacgggtta gagttaatga cgatttcata ggccagccct tgctgaccgt gcttatacag
     9661 ccgttcagtc tcgataaact ttttaccgaa tgaccagtgc ggatagttaa ttggcatgcc
     9721 gacgctggaa taggcatcca tcatctgttc tgaggttatc acttcaatct ggtgcgggta
     9781 ggtatccagc cggtagagtt tcgccacccg gtctatctct gccagataaa catccagcag
     9841 gtcgaacgtc cagtcgggtc catcgctcaa acgtgtggtg tccttattca tagaatcgat
     9901 cgtcgccata cgcgcacctc attgttgtcg gcgctctctg tgtggagcac ctcatttcaa
     9961 gcatagaaca cctgttaaaa accgcgtcgc cggagaattt ttttctttgc gatttcttat
    10021 tatcagagtg ccactaatcc gcttctgaac ggaattttat gctggataaa aagggcgttc
    10081 agcaggagat actaaagacg ccatattgcc gcagagtcag ggagatgtga gtcagctcac
    10141 cataaaaaag ccgtatgttg aataatattt tcaactgagt tatcaagatg taattagatt
    10201 attattcttt tactgtatct accgttatcg gagtggctat gcgagttgtc atactgggaa
    10261 gtggtgtggt aggcgttgcc agcgcctggt acttaaatca ggcaggacat gaggtcaccg
    10321 tcattgatcg ggagccgggg gccgccctgg aaaccagtgc cgcgaatgcc gggcaaatct
    10381 cccccggata tgctgcaccg tgggcagcac caggtgtgcc tttaaaagcg attaaatgga
    10441 tgttccagcg ccatgcgccg ctggcggttc gtctcgacgg tacgcagttt cagttgaaat
    10501 ggatgtggca aatgttacgt aactgcgaca ccagccacta catggaaaac aaagggcgga
    10561 tggtgcgtct ggcggaatac agccgtgatt gcctgaaagc attgcgcgcc gaaaccaata
    10621 ttcagtatga agggcgtcag ggtgggacgc tgcaactgtt ccgtaccgaa caacagtatg
    10681 aaaatgcgac ccgcgatatc gccgtgctgg aagatgccgg cgtaccgtat cagctgctgg
    10741 aatccagccg cctggcggaa gtggatcccg cgctggcaga agtagcgcac aaactgacgg
    10801 gcggcctgca gttacccaat gatgaaaccg gagactgcca gctgtttacc cagaatctgg
    10861 cgcggatggc agagcaggcg ggggttaaat tccgctttaa tacgcccgtt gaccaactgc
    10921 tttgcgacgg cgagcaaatc tacggcgtga agtttggcga tgaagtgatt aaggccgatg
    10981 cgtatgtgat ggcgtttggt tcttactcga cggcgatgct caaaggcatt gttgatattc
    11041 cggtgtatcc gctgaaaggc tactcgctga ctattccaat tgcgcaggaa gatggtgcgc
    11101 cagtatccac cattcttgat gaaacctaca aaatcgccat tacccgtttc gataaccgca
    11161 ttcgtgttgg cggaatggcg gaaattgttg gttttaacac cgagctgttg caaccgcgtc
    11221 gtgaaacgct ggaaatggtg gttcgcgatc tctatccacg cggcggtcat gtcgagcagg
    11281 cgactttctg gacgggtttg cgcccgatga cgccagacgg tacgccggtt gtcgggagta
    11341 cacgctttaa aaatctgtgg ctgaataccg gtcacggcac gctcggctgg acgatggctt
    11401 gcggttccgg tcagttgtta agcgatctgc tctctggtcg cacgccagcg atcccatatg
    11461 aggatctaag cgtagcgcgc tacagccgtg gatttacgcc atcacgtccg ggccatttac
    11521 atggcgcaca cagctaagga aacgagatga cccgtccgat acaggccagc ctcgatctgc
    11581 aggcattaaa acagaatctg tccattgtcc gccaggccgc gccgcacgcg cgcgtctggt
    11641 cggtggtaaa agcgaacgct tacgggcatg gtattgagcg tatctggagc gcgctcgggg
    11701 ccaccgatgg ctttgcatta cttaacctgg aagaggcaat aacgttacgt gagcgcggct
    11761 ggaaggggcc gatcctgatg ctggaaggat ttttccatgc tcaggatctg gagatttatg
    11821 accagcaccg cctgaccacc tgcgtacaca gcaactggca gctcaaagca ctgcaaaatg
    11881 cgcggctaaa agcaccgttg gatatttatc ttaaagtgaa cagtgggatg aatcggttgg
    11941 gcttccagtc cgatcgcgtg cttaccgtct ggcagcagtt gcgggcaatg gcgaatgttg
    12001 gcgaaatgac cctgatgtcg cattttgctg aagcggaaca tcctgatgga atttccggcg
    12061 cgatggcgcg tattgagcag gcggcggagg ggctggagtg tcggcgttcg ttgtccaatt
    12121 cggcggcgac tctgtggcac ccggaagcgc attttgactg ggttcggcct ggcattattt
    12181 tgtatggcgc ttcgccgtcc ggtcagtggc gtgatatcgc caataccgga ttacgtccgg
    12241 tgatgacgct aagcagtgag attattggtg tccagacgct aaaagcgggc gagcgtgtgg
    12301 gctacggcgg tcgctatact gcgcgcgatg aacagcgaat cggcattgtc gccgcaggat
    12361 acgccgacgg ttatccgcgc catgcgccta ccggtactcc tgttttagtg gacggcgtgc
    12421 gcaccatgac ggtggggacc gtctcgatgg atatgctggc ggttgattta acgccttgcc
    12481 cgcaggcggg gattggtacg ccggttgagc tgtggggcaa ggagatcaaa attgatgatg
    12541 tcgccgccgc tgccggaacg gtgggctatg agttgatgtg cgcgctggcg ttacgcgtcc
    12601 cggttgtgac agtgtaactt gttgtaagcc ggatcggagg cagcgtcttc tggttgcgaa
    12661 aaaatcatcc atccggctgg tcagcaactg tagttgttaa tgtgacagag ccattgccca
    12721 tgatagtgtc cattaaaaga atggacacta tttccccgga acctgaactc gccgcacagg
    12781 cgttctacat aaaacgctta cgcttcattg ttgactcgga gtcgacttca gataaatcac
    12841 gcttcaccct tgatgcagat tcgggttcat gttcagattg ttgattaaca ggctgcgagt
    12901 cctgttgtct ttccagactc aacaggccca actgttgatc caggagctcc aggtcctcct
    12961 gtaccttcac gcgctccagg agttccgggt catccaggaa ctccagatcc ataaggtcct
    13021 ccagctcctc gagtccttcc aggtcaacct agccttcctt gtgcaaaaag tccaaaaggt
    13081 ccttcaggcc ctgctgtttt ttcaggccct cgacggcttt gagcagcgtc ggtaaattgt
    13141 ctttgtccca cgggttacca ttacagtcaa acaccttcag gctctggggc tgcggcacct
    13201 gtaccgtttt aaacttgttt tctccacagt agtaaattat tcgtttgggc ggcagccgtg
    13261 gtgaagagat caaatcgtta ctagaacagt caaatacttt caggcgagtc aagttctcca
    13321 gcatctcatc aggtattgag ttaatattgt tgccggcaca ggatagttta tacaggtact
    13381 ggggcatcga cggcggtaaa aattgcaaga agttttcaga acagttaatc actctcaggt
    13441 tagtcgacag ctcaggcagc cagataatac tgttttcata gcacttcagt accatcagtt
    13501 ttgagggctg caccttcggt aacaagatca agacgttatc tgtacagtca agcaccagca
    13561 gccactttgg catcaccttc ggtaacgcgg tcaacacgtt tttactgcag taaaattcaa
    13621 tcagatgtgg aggcaaattc tcagggagtg aagtcaatcc caggttgcta agattgagca
    13681 tactccgtct tgtaatgaga cattccttca ttctgcgcaa tgcttctgcc cggttttcaa
    13741 tccggcttgc tggcaggttt tcttccagga tattttcctt gatttcttcc agactttctt
    13801 tcaggtcttc ttggggaatt tctgactgtt tgttttgctg gttttcttcc aggatttcgt
    13861 acaggttttt ttgcaggttt tcttgcaaac catcctgctg tatagcccat tcgcaccatt
    13921 tggcgaccat catgaactcg ttatgttgct gattcagacg agtccccaac gtgatgatgg
    13981 tattgttgtc cacctgtatg ttcactttgc ccgcgatact gaggtcaatg atctgccagg
    14041 gggcatcacc ccagctgaat ttttgcgggc tgagatactt catgttctgc aaaatttgat
    14101 tcttgagaag agccgccctg gcgggcgtcg tacgcctggg ttaacggaag atgggggaat
    14161 ggcgggggcg aatgggcaga tgtctttaaa gctagccata atgctacgtt tccttgaaat
    14221 aaatatcctc tggcagcgcc ggagacgtga atgtggtcac cgcaaaggag ccacaggacg
    14281 tcatttgcgc tgatatcact actcatcgtt gactttttcc ctggtcagaa atgccgtgtc
    14341 gggtctggta catgctgtat tacggcctag gtagctctga cgtattaaag aaaatacgag
    14401 agtgaacgac ccggaaataa tcgttattaa gcaaaggaat aactataaga aatcgctcat
    14461 gcatccggtg ctacaggcgg atgaaacgcc tattgtgtgg gctatgtcag tgcggcaggt
    14521 tgcgtggggg aatatgttca ggcgatgtca ggcaggggcc cctgatggtt gaccggtaat
    14581 gcggggctgg tttttattac ctgttacagt cggaaaaagc ccttacgctg actttcttga
    14641 gccggtgttt tgagttcatt agccagagcc tgcaatggtt tgtcatccat aatttaacct
    14701 tcttctgatg ttgggatgag catatcaaaa tcaggaaatg acaagtttat tacaggctca
    14761 ggctgcgttc agtaaagcat cgcatccggc gatagatgct gcattaaatt taagattcag
    14821 cttcctcttc agccacccga acgccaatct tcaacacttc attatcttct ttctcggcca
    14881 ccgtccagat catcccggca aactctacct ggtcaccgac aaccggtgcc gcgcctagca
    14941 actgctggac aatttcaccc agcgtttgct gtttatcacg atattctcgc ccgtcttcca
    15001 ggccatatat cagcgcaaca tcagcatatt tggcgctggc ttcgagaatg aagtcaccaa
    15061 agaagcgttg atccagcgcg accggtggcg actggctgaa cagtttaccg agcgccggga
    15121 gatcgcgttc ccgaccaatt acacacaata catcgccttc gcgcagtcgg gtgctgccgg
    15181 taggatgaag caactggtta tcacgaaaca gggctgcaat acgcgtctct tttggcatat
    15241 gcaaatcgcg cagtgccgca cctacacacc atttatcagc actcaattga taaacaaact
    15301 gctcccacgg attttccgga tgaatatcca ggccaacgcg tgacaccgga cgtccgacgg
    15361 gcggaaccac cactttggct tttttcgccg cccacgagag tgatgttccc tgcaagagca
    15421 gtgaaaccag aaccacaaag aaggcgacat taaagaacag tcgcgcattc tccagccccg
    15481 ccatcatcgg gaacacggct aggatgattg gcaccgcgcc gcgtaatccc acccagctga
    15541 taaacacgcg ctcacgcaga ttgaaaccgc ggaaagggag caatccagca aataccgaaa
    15601 ggggacgggc gaagaatatc atccatgccg acaaaatgag cgccggaatg gcaataggca
    15661 gcagatcgct tgggttaacc aatagcccca gcaccaggaa catggcgatt tgcgccagcc
    15721 aggcgaggcc gtcgaaattt tgcaggatgc cgtagcggtt gcgaatcggc cgattaccca
    15781 gcagaaaacc gcacagataa acagccagaa taccgctgcc ttccagcgcc gtggttaaag
    15841 caaaaattaa aataccgccg cttaatgcca gcaatggata taatccggca ggcagggcga
    15901 tgcgattaat catttgcagc agtaaataac caccgccaag accgatgaca atcccgaggc
    15961 caaattgctg cagaatatcg accacgaaca tccaactgac actgctttca tgctgctgga
    16021 tcattgcaat cagggtaatc gtcagaaaga ccgccattgg atcattactg ccggattcaa
    16081 tttccagcgt cgagccaaca cgttcgttaa gccccttacc acccagcaga gaaaagaccg
    16141 ctgcagcatc ggtggagccg acgatagcgc cgattaataa gccttcaatc aaatcaagat
    16201 taaacagcca cgccgccatc atgccggtta aaccagaggt gataagtacg cccagcgtcg
    16261 ccagcgacag cgccggtcct aacgccacac gaaaggaact ggcctgagtg cgcatcccgc
    16321 cgtcgagcaa aataatcgcc agcgccaggt tacttaccat gtaggcgaag gggtaattat
    16381 caaacgggat accgccgacg ccatcgactc ccgccagcat gccgatcgcc agaaaaataa
    16441 ccagaatagg aatgccaaga cgggaagaaa atgaactaag taatatactg ctggtgacaa
    16501 ggatggatcc taagatgaaa aggctaatta ttgttgtggc atccaacggt cggttactcc
    16561 tgattacgct gtctcttata taaaccctac catattagcg gcagagacag cgttttactt
    16621 agccctgaag cgtgattttt ttacatttta agaacaggat gaccgctaat cgtcaactga
    16681 gcgccttccc gggtgttatt cagaatggca tgtgcgccca acggcagcgt aaccgtgcgt
    16741 tgttcatgac caaaatcgag gccggtaatg agtggaatcg acaggcggga acgcaaaaac
    16801 gcgtacactg actccaggtt gtaacctgcg tcataatcat tgggcgtgct gccgctaaag
    16861 ctaccgagaa taatcgcctt ctgacgtggc aaaattcccg catgatagag ctgcaacagc
    16921 atacgttcga cgcggaaagg gtgcacgtta atatcttcca gcaccagaat accgttctca
    16981 atttttggca tccacggtgt accaatcagt gaaatcagca tcgcaagatt gcctccccac
    17041 aacgtgcctt cggtctgaca tgtcgggcct tccccttgcc attcaatggt gaaggtttca
    17101 ttgcgtaacg ccagccagaa atggtgctcg gtaaaggcgt tcagttcatc cgcaccaaag
    17161 tttgctacca gcatggggcc gctaaaggtg atgacattgc cttgcgccag aagaccgcac
    17221 tgaatggcgg taaaatcgct atgtccgcaa atgagtaacg ggtcatgttg ttggcgagcc
    17281 accagtgcct gccagtcaat atctgccagt aaacgactgg caccgtaacc gccgcgtaca
    17341 gccagcacga tggtgttggg ggtagtcagt ctggcaaggg aattgagatc ttccagacgc
    17401 tccgtttccg tacccgcaaa acgctcacaa cgacgggcaa taacctcgac gttattgact
    17461 tgatgccccg cgtcggtaag gcgctggata ccacgcagcg cggcgtgctg tttaatgctg
    17521 taacccgatg gggcaattaa gtgaaacaga gacatggcaa ttccttgctg acaacagaaa
    17581 cgaaatgtat atcatgccgc ttaggtgtgc cgttgtcacc tcaacggcga ttccaggcta
    17641 taaggataga agaagtgaaa ttgagatggt ttgccttttt gattgtgtta ttagcgggtt
    17701 gttcatcaaa gcatgactat acgaacccgc cgtggaacgc gaaagttccg gtgcaacgtg
    17761 cgatgcagtg gatgccaata agccagaaag ccggtgcagc ctggggcgtc gatccacaat
    17821 tgatcacggc gattatcgct atcgaatcgg gtggtaatcc gaacgcggtg agtaaatcga
    17881 atgccattgg tttgatgcag ttaaaagctt caacctccgg acgtgatgtt tatcgccgta
    17941 tgggctggag tggtgagccg acgaccagcg agctgaaaaa tccagagcgt aatatttcaa
    18001 tgggggcggc ttacctgaat attctggaaa ccggcccgct ggcaggcatt gaagatccga
    18061 aggtactgca atatgcgctg gtggtgtcat acgctaacgg ggcaggtgcg ctgctacgga
    18121 ctttctcgtc agatcggaaa aaggcgatca gcaaaatcaa cgatttggat gctgacgagt
    18181 tcctcgatca cgtagcgcga aatcatcctg cgccgcaggc tccgcgctat atctacaaac
    18241 ttgagcaggc actggacgcg atgtaaatca gtcgcgcact ttgtccgctt tttcccgggc
    18301 ttctcgctcg agagagaaaa taatccgctg taattgccgc tccaccgtcg ggctgacgtt
    18361 aagaaaacgg aagctcagac ggggagtggt gatggtttca ttcttgccat caatcacttt
    18421 gcgctcgctg gtggagatta actgggcgtc aaagtgaaaa acaccccatt gccccatgtt
    18481 gacttcaatt tgagcgaagc gcataccttc ttgtaattcg gcaggctttg ctgtttccag
    18541 taatgcgccc atgccgccta acgacaaatc atacaagcgg aaacgtaacg tactgttatc
    18601 cgccagtttg gtctggcaaa aataaggcgg atggagtggg gcggagatgc ggaaatatcg
    18661 gcgtcgttgt acaaaccata aggtgggagg cggtacggta ataaatgccg gaagctgcaa
    18721 gtattcactc tgctgtagtt gttcaacagt aaactcgact ttcgcaccct gagtttcggc
    18781 ggtaatggta atgtgctgtg cctttagcac ggcgatgttg tcttcggctt gactgccgaa
    18841 atccagcacc agtttatccg gggttattgc cagtattttg ctgatcagct gcccgccatt
    18901 ccaactgaga cgcaaaggaa ttgcggtttt gtgcaaatcg cgtaacacgc ccaggacggc
    18961 taacggattt tgtttcagga actgctcatg gtaatgactc acgcggaaaa actcctgact
    19021 gacaaactgt ctttggttta tcggtcatag ttaacaaaac ttaatacaaa tgcgtgaata
    19081 ttttttacat gttgttctta aatcagccga caaacgccca gcatcgatcc cgcccttgcc
    19141 tatacttaga gcgttgacgt aagcatttct tgcgaccgat tcatcgaaac gagggcctga
    19201 acatgggaat tattgcctgg attatttttg gcctgatagc cggcattatc ggcaagctaa
    19261 tcatgccggg gcgtgatggt ggtggatttt tcctgacctg tattctcggg atagtcggtg
    19321 cggtggtcgg cggctggctg gcgaccatgt ttggcattga cggctccatc agcggtttta
    19381 atttgcacag cttcctggtg gcggtggtgg gcgctatcct ggtgctgggc gtattccgcc
    19441 tcctgcgaag agaataagat tttcataagg cggatagcga tacagatgcc gctatccgct
    19501 ttcacatcag aacgtatact cgacacctgc ccagaaattg cgaccttctt caataaaccc
    19561 ttcactgtag gcatattggg tatcaaacag gttattgacc gacgcattga cgctgaagcc
    19621 atgacctaag gtgtaatcgg ctcgaatgtg ggtcaccgca aaaccggtgg ctttttgtga
    19681 accgtcactg ttgctgtagc tggaggaacg cgcctcttcc gacagcgtta cgcttaacgg
    19741 ctcccatggt ttgagtgtca tccatgcggt cattgtctgc gttggaagat cggttatctt
    19801 gccgatgtct ttgcgtttgg cgtcagcgtg gatcagagcg tagctcaatc ctacatccag
    19861 tatattgctg attttcccct taataccggc atccagaccg ctgtaatcca ccgtgccgct
    19921 gttctgattt tgaatggtat cggcattgat attgtgcgag aggatggcat cactcacccg
    19981 gttatagtaa acgctgacct caaatcccca gtcgcgcgtg aaggcaccat tccaggttaa
    20041 atccaccccg cgagcgcgtt ccggcttgag ctgcgggtta acaatcgcta tctggttata
    20101 cgcaggtttg gatgtggtat agcgttcttt cagcgtcgga aagcgtgtgc ggtcatagta
    20161 cgaaagcgcc agagtgtctt cattggcaaa gtggtatttc cccatcacct gccagttaaa
    20221 agctgactga ttgttgtcgt cataatgggt gatgctgcca tctttctcgt gttttttcgc
    20281 ttctacgcta tcgcgccagt catagctgat tcccgccacg acatcgacat tatcagcagc
    20341 agcccattga tattcactgg cgagcgacca ggtacgatct tcatagcgat cgtaagcggc
    20401 gtgcggcgca cctttttccc ggtgtacgtc atctttccag ttaacggcaa acgacagcag
    20461 atcgttttca cgcacatcgg ctgccagttg taatccggca ccgtcgctgt aatcggaata
    20521 atggctgtag ctgccttttt tatttttcaa atcagccagc gagttgtaca tcatcagcgt
    20581 attttcaaag gtgtcgcgat acagccgact tttcagggta aaacggtcgt ttagttgggt
    20641 cgctccctga taataaaaac tttctttgtc atactctggc cactgccagt agcgtgattt
    20701 ttgaccacaa tttccgctgt atggcgggtt atctttttca ccatcctgct taatgtaagt
    20761 cagcgtgtat tcatcgtttt cgcgcggtgt aaaaccgagc ttcacaatgc cgcgtttatc
    20821 atcagccgat gaattaatca tcttgccgtg tttgcctgca atatcattat ttacaccatg
    20881 cggcaggccg agaaaatcct gctttagctg gctaccgctg acttgcaaat acccaagatc
    20941 gctgctggcg gcaaatgaag catgcatatc gtaggcattg tcctgactac ggctccatcc
    21001 ctggcgatat cccagacttg cttcttcagg gtggtgatat taatggctcc gcccatctga
    21061 ttaggcccat gaagcagcga cgaataccct ttggaaactt cgactgcccc cagattgttg
    21121 gtcagaatcc gcgccagatc gaggttgccg tcatagggga cataaatggg cacaccgtcg
    21181 aaatagaccg gcacctgccg actatcaaag ccacgaactt ttacctgttc ttcgttgcgg
    21241 ctgcctgact tttgcagcac cacgccgggg acgacactta atgcctgagc gacattctgt
    21301 ttatccaagg ccttcatggt gggttgatcg agaacggtgg tcgtcgtcga tgataccgga
    21361 ctggaccata cggtcagcgt gtcgctctcc tgagccgcta cggcctgctg ggtaaaggca
    21421 agtgataatg ctgtgcagag ataacgtttt ttgagtctca tagaataaat tccgtttaac
    21481 aaagaattaa atagaggctg tgggttgcag actgacttta atgtcagctg gtgccgcgta
    21541 ataaggcgcg gaggtaataa aaaggcgaat gccgcagtcg aggtaatttt tgagtgtcgt
    21601 cagattaatt ccgccggtca gagccagcgt gcagtgagga gccagcgacg gcgctatttg
    21661 cgcaatttca gttgcctgtt gcggactaaa tttgtcgagc tgaagtacgt ctggttgtgc
    21721 gcgtaacgcg gcgatagcct ctttcggcgt gtcggcttca acaacaattt tcttctcggg
    21781 tgcgtggcga cgtaactgag cgattgcgcc tgaccagccc tgattgtcat gaagaaaatg
    21841 gcggtggttg gcaaacaata atatggtttc cgcacatccg gcacgatgaa ttagtcctcc
    21901 ggcagccaga attgcctgcg aagccagtaa gcgggtgccc ggaattgctt ttcgggtgca
    21961 ggcgatattg ccgtcaggat aacgttcacg aagcaatgcc agcatttgag cgagataatc
    22021 agaaacaccg caactccact ccagcacatt ctggactgcc ttccatccct gatgaagtgc
    22081 tgcggcatta ccctgcgcac ggattagccg ttgacccgcg ttcgcttgtg aaccgtcgct
    22141 gaccgcgtca tcaatagtta atcccagcgt agttaacatc ttacacgcga cagaaacgcc
    22201 gctgacgcag ccgccctgac ggtgaaaaaa cgctatatag ccaggctggt gtccaatatt
    22261 taatgctcgc gtggtcaggt cacctccctg gatatcttcc agcagtaacg catcgatttg
    22321 tgcctgagaa aggaaaatca tcacagtgcc tcctgcggta cgctatccca ccagatgagc
    22381 gcccagtcgc ggctggcagg ggcgagggca cgagcatctt gttgttgata ccagtgatat
    22441 aaccgctcac gctcatcgtc gttaagcgcg ccaagactcc agctgacgtt ctgctcaaaa
    22501 cgttcccagg tgctgttatc ctgttggcaa ttttgtccac ggataaaatc aacgtgggca
    22561 taaattccca tctgatacag gacgttgagc gcaaagatat agttgggtaa ttcgatcact
    22621 tcccgaccag ccgcccgctg aatggctgga gagacgaacg aggtgctgac cagatgagtt
    22681 gtatagacgc gaagccgtgc ctgattattc agtttgctca ttgcatggcg catatcagcc
    22741 accagagtgg agcgggaagc cacggcaata tcgcagcgtg gcaggtcact ccagtcctct
    22801 tcccaggcgc gctggatcca gtggacattg tcagccttca gtgcggcggc gcggcgggcg
    22861 gcaacgttca gcatcccctg actgtaatca acgccataaa ccgtagtgag tttgtcagcc
    22921 agcgccagcg atacggtgcc tggtccgcag cccatatcga ataaggtctg agcgccttgc
    22981 aggtcaattt tagcgatcag ttgttgcagg tagctgtccg ttggcgaggc gcaattttcc
    23041 gccattttct ccgcccgttg atcccagtga tcgggcgttt tttctgtgcg gtgagccagt
    23101 ttgagttgct gcagataaag gtctgcaaaa tcaatatcat caatgagcat gattgttcct
    23161 tagcggctaa ttgcagagag gtgatgatga atttggtcgg cagaaacccg atacagggcc
    23221 gccagtttgt tcgttgttaa ctgttcggtc ggtaagcctt gtgttactcg tccatctggc
    23281 tctacttgga taatgctgtc ggcaatggcg ttggcatgga gcggatggtg agtcgacatt
    23341 aacattgtca tgccgttatt tttgagctgc gccagcgtgt ccagaagttg gatctgatga
    23401 ccgaaatcga ggctggaggc gggctcatcc agtaacaaca agcgcggttg ttggacgagt
    23461 gcacgggcga ttaaagccag ctgacgttca ccgccgctaa gcgtattcca gcgtcgtgtc
    23521 gccagatgca aaatgcccag cttttccagt tgctccgtgg ctttcaggcg ttcttgctta
    23581 ccgggaacgg aaaaagcgcc tatggtgggg gccaggccca tcaacaccat atccagcaca
    23641 ctgaaagcaa aaataccgtc gtgcgcctgc ggcacccagg caatagcctg tgcgcgctgg
    23701 cgatggctga gtgtctgcac agagaggcta tccagtagga tctcgccttt taataacggt
    23761 ataacgccaa gaatggagcg catgagtgtg gttttgccgc aaccattcgc gccaagcaga
    23821 caacaaattg ttccttgcga cagggtgaag gacacgtcgc gtagcactgg atggcgggag
    23881 taacccagag acgcatgacg aacggcaagc aaagtcactg gcgacctccg cgtaacaaca
    23941 aaagcaggaa aaacggtgcg ccaacaaaag cggtcagaat gccaagtggg atttctgttg
    24001 ttccaatgct gcgcgccagc gtatcggtaa gcaggagcag aatagcacca gtacacattg
    24061 ccataggcaa cagctgctga tggttatgac cggtcaacaa gcgggcaatg tgcggaacga
    24121 ccagaccgat ccaaccaatt atgcccgcta ttgctaccgt gctggcggtg attagtgtgg
    24181 cgcagacaat caatccaaag cgcagacgcg tcacgttcag tcccagcgaa cgggcttcgt
    24241 cgtcggaaag cgtgagtaaa ttcatgcgcc agcggagaaa aaacaggggc aaggagccta
    24301 tgagaatgat cggtgcagca tagcagagat cgcgtaaggt cacggtggac agtcccccca
    24361 gcaaccagaa ggtaattgac ggtaactggg tgtaaggatc ggccagcgtt ttaattagcg
    24421 atatccccgc tccgcacaac gttcctaacg caatgcctac cagcaccagg gttaagatcg
    24481 gatcgtggcg ggtcacccga cgcgtaatca accagacgcc cgcgaccacc atcagcccgc
    24541 cgcaaaatgc gtacagctgg atgtaaacaa tgggtaatcc ccacaggatt gccgcgcagg
    24601 caccaagtcc ggcaccggcg gagacgccta aaatatccgg tgaaactaag ggattacgca
    24661 acattccctg ataggtggtt ccggctcctg ccagcgctgc gcccagtaac agcgcagcaa
    24721 gaatacgcgg cacgcggatt tgccagaaga caatttgttc ctgcacagac tcattttctt
    24781 gacgaagaat tagcgccagc agttgctgca tatcgagatg ataagcccca cttacggcgg
    24841 caaaagtgat actgatagct gccagcagca aaatacatcc gtttacgatt ctcattagct
    24901 cgccaccaat ttttgatagt cggcatcact gagtgaacaa tgccagaaca gttgggcgta
    24961 atgctgcatg tcacttttaa actggcgatt gatggcggga tccagccagg cgtgaagtct
    25021 gcgtaatccc agaagacggt tgattcccgg cggggcatcg agccagccaa agggcaagcc
    25081 acttaaaaat aagatgcgtt gttccgcaac ggctttcacg ccctgccaga gaggatcacg
    25141 acgaataaaa tctgcagtaa cggcctcctg aaccagaata atatccggct gccagcgcag
    25201 gagattttcc atggaaacct gtgtcaggcc gtggcgatcg gctatttgcg cgacgttgtg
    25261 caaacctaat aattccgccg cctcggtatg caacgaaccc tgtaagcccg tttccagccc
    25321 tcggggaccg cgcgcagcat aaaagcggag gttagcggcg ggtgaggtgg cgaatgcctg
    25381 agcttcacca acgaagtggc tggcgagatt ggcttgttct gcggcgcggt gctcttcgcc
    25441 taacgttttg ccaagcgttg ttaactgttc tgctgattgc gccagttttc cgttaagcaa
    25501 taaccagggg atttgtgtct gttcgctaac ctgccgtgcc tgggagatca aggtttcatc
    25561 cgtattgccg caatcaacga ccaaatcggg atgtaacgcc ataagccctt ccagcgatag
    25621 tgtgctggcg cgcccggcga gtcgtcccag cctgggtaac tggcgaatgt gctctggcaa
    25681 tggaattaat gcctgacggg caaaatcaaa agaggaaaaa ccgaccattt tttctggcgc
    25741 gacagccagc aagagcaaat cggcgggcgc gcctgcgctg acgatgcgtg taatgttttg
    25801 ggcttcggga agagattgcg aagcaaaacg attaactgtt tgtgaaaagg acggtagaga
    25861 ctgcaggagc agggtagatg caagtgcctg cgcaaacgtc cgtctggtta tgggcatggt
    25921 gaacatcctg aaaagtaaaa tcgatatata ctttcatata tatcgataaa aatcatgttc
    25981 aatatcaaag tttagctatt gaaaacatga agatgtgacg acttccgcaa gcctgttgac
    26041 cctctctatc attaaaaatg tatttatcag gtggctaata ttatttaaat catttaaggc
    26101 taaatcattt aaatatgaat gacattattg ttttttatcg tccacggtta cgcgacgttg
    26161 aagttacttg ttttataatg agcgattttt gatatctcgc cgagaaaatt atcagcattc
    26221 tctcgtttca gtgtcgtgaa tatactgtta acggaggtga aaagaatgct gggggttata
    26281 caaaacctat gttgttgtcg tccggattca ccgcttgctg atagtaagca gcgtaaccag
    26341 aagttaccgc aacaagactt tgaggatttt aagaacgacg ttattaatgt cattaacaac
    26401 aatgaagata tttattttct tagagagagt aatttactga atgcgtttat tcggacgtgc
    26461 aaaaaaagag atgataacag ttacggaaat atgatattta cgctttattt tgataacgat
    26521 atctatggga acagaattat caaagcatcg ctgaacggcg gagagttgca gttattaagc
    26581 acagggtgta gcaatgataa cattctc
//
LOCUS       DAOFMX010000060        24639 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.060, whole genome shotgun sequence.
ACCESSION   DAOFMX010000060 DAOFMX010000000
VERSION     DAOFMX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.060"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(1..374)
                     /locus_tag="QBA20_002762"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="QBA20_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..2826
                     /gene="thrS"
                     /locus_tag="QBA20_002763"
     CDS             898..2826
                     /gene="thrS"
                     /locus_tag="QBA20_002763"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDQ6293235.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2830..3372
                     /gene="infC"
                     /locus_tag="QBA20_002764"
     CDS             2830..3372
                     /gene="infC"
                     /locus_tag="QBA20_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDQ6293236.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3469..3666
                     /gene="rpmI"
                     /locus_tag="QBA20_002765"
     CDS             3469..3666
                     /gene="rpmI"
                     /locus_tag="QBA20_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDQ6293237.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKTTKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3719..4075
                     /gene="rplT"
                     /locus_tag="QBA20_002766"
     CDS             3719..4075
                     /gene="rplT"
                     /locus_tag="QBA20_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDQ6293238.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4192..4316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4198..4242
                     /gene="pheM"
                     /locus_tag="QBA20_002767"
     CDS             4198..4242
                     /gene="pheM"
                     /locus_tag="QBA20_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDQ6293239.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4525..5508
                     /gene="pheS"
                     /locus_tag="QBA20_002768"
     CDS             4525..5508
                     /gene="pheS"
                     /locus_tag="QBA20_002768"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDQ6293240.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5523..7910
                     /gene="pheT"
                     /locus_tag="QBA20_002769"
     CDS             5523..7910
                     /gene="pheT"
                     /locus_tag="QBA20_002769"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDQ6293241.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7915..8214
                     /gene="ihfA"
                     /locus_tag="QBA20_002770"
     CDS             7915..8214
                     /gene="ihfA"
                     /locus_tag="QBA20_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDQ6293242.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8315..9295
                     /gene="btuC"
                     /locus_tag="QBA20_002771"
     CDS             8315..9295
                     /gene="btuC"
                     /locus_tag="QBA20_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDQ6293243.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9358..9909
                     /gene="btuE"
                     /locus_tag="QBA20_002772"
     CDS             9358..9909
                     /gene="btuE"
                     /locus_tag="QBA20_002772"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDQ6293244.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVALGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9909..10658
                     /gene="btuD"
                     /locus_tag="QBA20_002773"
     CDS             9909..10658
                     /gene="btuD"
                     /locus_tag="QBA20_002773"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDQ6293245.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10736..11200
                     /locus_tag="QBA20_002774"
     CDS             10736..11200
                     /locus_tag="QBA20_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ6293246.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11447..12160
                     /gene="ydiV"
                     /locus_tag="QBA20_002775"
     CDS             11447..12160
                     /gene="ydiV"
                     /locus_tag="QBA20_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDQ6293247.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12224..13660
                     /locus_tag="QBA20_002776"
     CDS             12224..13660
                     /locus_tag="QBA20_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDQ6293248.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13664..13855)
                     /gene="hemP"
                     /locus_tag="QBA20_002777"
     CDS             complement(13664..13855)
                     /gene="hemP"
                     /locus_tag="QBA20_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDQ6293249.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13987..15033)
                     /gene="aroH"
                     /locus_tag="QBA20_002778"
     CDS             complement(13987..15033)
                     /gene="aroH"
                     /locus_tag="QBA20_002778"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDQ6293250.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15190..16023)
                     /gene="ppsR"
                     /locus_tag="QBA20_002779"
     CDS             complement(15190..16023)
                     /gene="ppsR"
                     /locus_tag="QBA20_002779"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDQ6293251.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16355..18733
                     /gene="ppsA"
                     /locus_tag="QBA20_002780"
     CDS             16355..18733
                     /gene="ppsA"
                     /locus_tag="QBA20_002780"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDQ6293252.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18790..20436)
                     /gene="fadK"
                     /locus_tag="QBA20_002781"
     CDS             complement(18790..20436)
                     /gene="fadK"
                     /locus_tag="QBA20_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDQ6293253.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20493..20786)
                     /locus_tag="QBA20_002782"
     CDS             complement(20493..20786)
                     /locus_tag="QBA20_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDQ6293254.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20783..22072)
                     /locus_tag="QBA20_002783"
     CDS             complement(20783..22072)
                     /locus_tag="QBA20_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDQ6293255.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22128..23066)
                     /locus_tag="QBA20_002784"
                     /pseudo
     CDS             complement(22128..23066)
                     /locus_tag="QBA20_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(23086..23850)
                     /locus_tag="QBA20_002785"
     CDS             complement(23086..23850)
                     /locus_tag="QBA20_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDQ6293256.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24166..>24639
                     /locus_tag="QBA20_002786"
     CDS             24166..>24639
                     /locus_tag="QBA20_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDQ6293257.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLA"
BASE COUNT         6102 a         5948 c         6477 g         6112 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      181 cgacagtttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taagtcagag
      241 gtatcaagta gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt
      301 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
      361 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      421 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      481 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      541 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      601 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      661 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      721 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      781 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      841 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      901 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      961 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     1021 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1081 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
     1141 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1201 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1261 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1321 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1381 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1441 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1501 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1561 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1621 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1681 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1741 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1801 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1861 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1921 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1981 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     2041 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2101 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2161 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2221 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2281 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2341 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2401 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2461 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2521 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2581 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2641 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2701 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2761 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2821 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2881 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2941 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     3001 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3061 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3121 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3181 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3241 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcagta
     3301 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3361 aagaaacagt aaggccttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3421 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3481 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3541 aagcacgcta acctgcgtca cattctgact aaaaaaacga ccaaacgtaa acgtcacctg
     3601 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3661 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3721 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3781 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3841 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3901 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3961 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     4021 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4081 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4141 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4201 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4261 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4321 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4381 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4441 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4501 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4561 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4621 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4681 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4741 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4801 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4861 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4921 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4981 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     5041 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5101 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5161 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5221 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     5281 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5341 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5401 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5461 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5521 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgata
     5581 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5641 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5701 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     5761 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5821 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5881 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5941 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     6001 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     6061 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6121 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     6181 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6241 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6301 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6361 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6421 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6481 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6541 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6601 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6661 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6721 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6781 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6841 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6901 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6961 aggcaagcct gattatgggt actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     7021 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     7081 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     7141 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7201 agaaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7261 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7321 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7381 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7441 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7501 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7561 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7621 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7681 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7741 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7801 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7861 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7921 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7981 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     8041 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8101 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8161 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8221 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8281 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8341 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8401 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8461 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8521 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8581 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8641 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8701 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8761 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8821 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8881 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8941 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     9001 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     9061 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9121 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9181 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9241 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9301 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9361 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9421 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9481 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggcgctg
     9541 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9601 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9661 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     9721 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9781 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9841 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9901 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9961 cgctttcagg cgaggttcgg gctggggaaa tcctgcacct ggtggggccg aatggcgcgg
    10021 gtaagagtac cttactggcg caaatggccg gaatgaccag cggtaaggga agcattcagt
    10081 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    10141 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10201 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10261 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10321 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ttgcttcttg
    10381 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10441 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10501 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10561 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10621 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10681 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10741 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10801 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10861 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg gtgtggactg
    10921 ttcgggattt gtggttgtga cgatgcgcga tcgtttcgat ttgcatctgc cccgtgaaac
    10981 caaacaacaa gcctctattg gcacgcaaat tgataaagac gagttgctgc ctggtgacct
    11041 ggtctttttc aaaacgggtt ccggacaaaa tggtttgcat gtaggtattt acgataccaa
    11101 caaccaattt atccacgcct ctaccagcaa gggagtgatg cgttcctcac ttgataatgt
    11161 ctattggcag aaaaatttct ggcaggcgag acgaatctag tatgtcgaaa aattgacggc
    11221 gatgatacat cgccgtcaat ttactcctga atacagtagt attttgcaga caaaaattcc
    11281 gtaaatggat tattcgttta taatcaggat aatctaaaaa aaggccccct gaaaaagtgg
    11341 ttaacatgga acaaatgaaa gttactcctt ctgtaattgt tatgtcttat ggcctcatcc
    11401 cttttagccg gatactgaaa aacatccttc gagagggacg gttaccatga agattttttt
    11461 ggagaatctt tatcattctg attgctattt cctgccgata agagataacc agcaactcct
    11521 tgtcggcgtt gagttaatta cccatttctc cagtgaagat ggcacggttc gtatcccgac
    11581 gagtcgggtt atagcgcaac ttaccgagga gcaacattgg caattatttt cagagcagtt
    11641 ggaattacta aaatcatgcc aacacttttt tattcaacat aaactctttg cctggcttaa
    11701 tttaacgcca caagtggcta cattattatt agacagggat aattacgcag gcgaactatt
    11761 aaaatatccg tttattgagc tgttgattaa tgagaattac ccgcatctca atgaaggaaa
    11821 agataatcgc gatcttttgt ccttatcgca aatgtatccc cttgtgttag gcaatttggg
    11881 agcaggtaat agcacaatga aagcggtttt tgatggtctt ttcacccgcg taatgctgga
    11941 gaaaggtttt attcagcaac aaatcacgca tcgctccttc gaaccgttta ttcgtgcgat
    12001 ccaggcgcaa atttcccctt gttgcaattg catcatcgcg gggggcattg atacgccaga
    12061 aatattggca cagattaccc ccttcgattt ccatgctctc cagggatgcc tgtggcctgc
    12121 cgttcctata aatcagataa cgacgttggt tcagcgataa ccctcctgtt tgctggtgtt
    12181 taagacgaga gtaaccgtct acactatcaa acagatagga tctatgaccc tgtcttttat
    12241 tacccgctgg cgcgatgaat tgccagaaac ctatacagca ctttccccta cgcctttaaa
    12301 taatgcccgg ctgatttggc ataataccga actcgctaac acgctgagta ttccatcgtc
    12361 gctgtttaaa aatgccgcag gtgtctgggg cggcgaaacc ctactgcctg gtatgtcacc
    12421 actggcacag gtttacagtg gtcatcagtt cgtcgtctgg gcgggccaac tgggtgatgg
    12481 gcgcggcatt ttacttggcg aacaactgct tgctgatggc actacaatgg actggcatct
    12541 gaaaggtgct ggcctgacgc cttattcgcg aatgggtgat ggacgggcgg ttttacgttc
    12601 gacgatacga gaaagtctcg ccagtgaggc gatgcattat ctgggcattc cgacgacccg
    12661 cgcgttaagt atcgtcacca gcgattcgcc agtgtatcgg gaaacggtgg agccaggcgc
    12721 gatgctgatg cgtgtggcac caagtcatct gcgctttggt catttcgaac atttttacta
    12781 tcgccgcgag ccggaaaagg ttcgtcagtt ggctgacttt gccattcgtc attactggtc
    12841 acatcttgaa gacgatgagg acaaataccg tctctggttt aacgatgttg tcgcacgtac
    12901 cgcgtcgtta attgcccaat ggcagacggt cggctttgca catggggtga tgaataccga
    12961 caacatgtcg ctgctgggac tgacgcttga ttacgggccg tttggttttc ttgatgatta
    13021 cgaacccggt tttatttgta atcactcgga tcatcaaggg cgttatagct ttgataatca
    13081 acctgctgtc gcgttgtgga atttacagcg tctggcgcag acattgtcac catttgttgc
    13141 cgtagatgcc ctgaatgagg ccctggacag ctatcagcag gttttgttga cgcattatgg
    13201 acaacggatg cggcagaaac tgggcttcat gacggagcaa aaagaggata acgcgctact
    13261 gaatgaatta ttcagtctga tggcgcgaga gcgcagcgat tatacccgca cattccgcat
    13321 gctgagtctg accgagcagc acagcgcggc gtcaccgcta cgtgaggagt ttattgatcg
    13381 tgcggcattt gatgactggt ttgcccgtta tcgggggcgt ttgcaacaag acgaggttag
    13441 cgatagtgag cgtcagcaac tgatgcaaag cgttaaccca gctctggtat tgcgcaactg
    13501 gttggcgcaa cgggcgattg aagcggcaga aaagggtgat atgatggaat tgcaccgcct
    13561 gcatgaggcg ttgcgaaatc ctttcagcga cagagatgat gactatgtca gtcgtccacc
    13621 tgactggggt aaacggctgg aagtcagctg ctcgagttaa aggctactta gttaacagca
    13681 gcttgccagc ctgagtttta cggagcagat attcttgccc gtcatgatcg ataatcagtt
    13741 tgccatccgg acctaacagt gtctggctgg taattcgccg gatgggctgc ggggaagcgg
    13801 tcttgtgatt ggcatccgtt tcaggcgtga gttttctgct atccgtataa cgcataaaaa
    13861 ttaataataa ttgatataac aatgattcct attatcaata ttatggcagg ccagagcaag
    13921 cagtaatgtg ttgagaagac aacaagttgc gcagaaatgc ccatcccatc aggaatgggc
    13981 acgcattcag aagcgggtat ctaccgcaaa ggcgagtttt tcgaccaggc gttcggtatc
    14041 ttcccagccc agacacgggt cggtaatgga ttgaccgtaa gtgagcggct gaccgccgac
    14101 gattttttgc gttccttcgc gcaggaaact ttccgccata attccagcaa tctccgtaga
    14161 gccattgcgg atttgctgac aaatatcctc acaaacttct aactggcgac ggtgctgctt
    14221 ctggcagtta ccgtggctga aatccaccac cagatgttca ggtaaatcaa actcgtgcag
    14281 cgtatcgcag gctgcggcga tatcatcggc atgataattc ggttttttgc cgccacgcat
    14341 aataatgtgg ccatacgggt tgccgctggt ctgatagatg gtcatctgac catttttgtc
    14401 tggcgagagg aacatatggc tggcgcgggc tgcgcggata gcatccacgg caatccgcgt
    14461 gttgccatcg gtaccatttt taaaacctac cggacaggag agtgctgaag ccatttcgcg
    14521 gtggatctga ctttcggtag tacgagcgcc aatcgcgccc caactgatta aatcagcaat
    14581 aaactgaccg gttaccatat cgaggaactc agtcgcggta gggacaccca gctcatttac
    14641 ctgtaaaagt aatttgcgcg ccagctccag accgtgattt acccgatagc tgccgtttaa
    14701 atccggatcg gagattagcc ctttccagcc gacaacagtt cgtggttttt caaaataggt
    14761 gcgcattacg atttccagcc gtgactggta ctggttgcgc agcgactgca gacgggtggc
    14821 gtactccatt gcagcggtga gatcgtggat cgagcagggg ccaataatga ccaacagtcg
    14881 cttatcttca ccattcagta ttttttcaat tctgcggcgg gagtcggtga catgggtggc
    14941 gacgccaggc gttacgggat accgtagcgc gagttcggcg ggcgttacca ggctctcaat
    15001 acgcgcagta cggagttcgt cagttctgtt cattacaagt ctcagaaatt tgttgcttct
    15061 acggcaggac taccggaaag tgatgcgcat cacgataaac caatcgcagc tgatttcaag
    15121 tgcgaggtgt gtcacacctc gccgggtggt tagcatgata acaaaaaaac aaaccaatgc
    15181 actagttctc tagtacattc ggcgactaag gcccatgata tcgaggatct tggtggcaat
    15241 ctcttctacc gaataattgg tactgttaat ccacgggatc tgatttttac ggtacaaggc
    15301 ttccacttcc gcgacttcca tcctgcactg acgaagcgag gcatagcgac tgttctcccg
    15361 acgttcctcg cgaatcgccg ccagacgttc cgggtcgata gtcaggccga acaatttatg
    15421 ctgaagcggt ttgagcgacg cgggtagcac cagattatcc atatcgtcgg caataaaggg
    15481 gtagtttgcc gcgcggatac caaattgcat tgccagatac agactggtgg gggttttacc
    15541 acagcgagaa acaccgagca ggatcacctg agcctgatcc agattgcgca acgaaatgcc
    15601 gtcatcgtgg gcgagggtgt aatcaatcgc cgcaatgcgc gcatcatatt tattgagatt
    15661 attagggtta aggccatggg tacgatgagc aatcggcgtt ggatccagtt tcatctcttg
    15721 ttgtagcggg gcaaccagcg cctgaacgat atcctggcaa aagccttcac tttgcaagat
    15781 gatggcgcga atctccggca acacgatgga gtagaagacc agcgggcgca cgcctgtctg
    15841 gtgataaatt gcgtcaatct gatccttcac tgcccgtgca cggctctcat tttcgacaaa
    15901 cggcagcgtg atgctgctga tagtgacggg aaattgtgac attactgcgt gtcctaatac
    15961 ctccgcagtt attgccgtac catcagaaat ataaaaaacg tggcgatcaa cagcattatc
    16021 cattttgttc ttcccgtgaa gcagacataa ttctatgaaa gcataaatta aaagcgcaca
    16081 gaagcgtagg acgtaatgtc tggtttataa aatgaacctt cattttattt tttataaaaa
    16141 cagcatttca tttttatggt ttcgtttata ccgatggttt atgtggaaat tgtcgaatag
    16201 agcaggtttg cgcaacgctg ggatcagtct taaaaagtaa aaaaatatat ttgcttgaac
    16261 gattcaccgt ttttttcatc cggttaaata tgcaaagata aatgcgcaga aatgtgtttc
    16321 tcaaaccgtt catttatcac aaaaggattg ttcgatgtcc aacaatggct cgtcaccgct
    16381 ggtgctttgg tataaccaac tcggcatgaa tgatgtagac agggttgggg gcaaaaatgc
    16441 ctccctgggt gaaatgatta ctaatctttc cggaatgggt gtttccgttc cgaatggttt
    16501 cgccacaacc gccgacgcgt ttaaccagtt tctggaccaa agcggcgtaa accagcgcat
    16561 ttatgaactg ctggataaaa cggatattga cgatgttact cagcttgcga aagcgggcgc
    16621 gcaaatccgc cagtggatta tcgacactcc cttccagcct gagctggaaa acgccatacg
    16681 cgaagcctat gcacagcttt ccgccgatga cgaaaacgcc tcttttgcgg tacgctcctc
    16741 cgccaccgca gaagacatgc cggacgcttc ctttgccggt cagcaggaaa ccttcctcaa
    16801 cgttcagggt tttgacgccg ttctcgtggc agtgaaacat gtatttgctt ctctgtttaa
    16861 cgatcgcgcc atctcttatc gtgtgcacca gggttacgat caccgtggcg tggcgctctc
    16921 cgccggtgtt cagcggatgg tgcgctccga cctcgcatct tctggcgtga tgttctccat
    16981 tgataccgaa tccggctttg accaggtggt gtttatcact tccgcatggg gccttgggga
    17041 aatggtcgtg cagggtgcgg ttaacccgga tgagttttac gtgcataaac cgacactggc
    17101 ggcgaatcgc ccggctatcg tgcgccgcac catggggtcg aaaaaaatcc gcatggttta
    17161 cgcgccgacc caggagcacg gcaagcaggt taaaatcgaa gacgtaccgc aggaacagcg
    17221 tgacatcttc tcgctgacca acgaagaagt gcaggaactg gcaaaacagg ccgtacaaat
    17281 tgagaaacac tacggtcgcc caatggatat tgagtgggcg aaagatggcc acaccggcaa
    17341 actgttcatt gtgcaggcgc gtccggaaac cgtgcgctca cgcggtcagg tcatggagcg
    17401 ttatacgctg cattcacagg gtaagattat cgccgaaggc cgtgctatcg gtcatcgcat
    17461 cggcgcgggt ccggtgaaag tcatccatga catcagcgaa atgaaccgca tcgaacctgg
    17521 cgacgtgctg gttactgaca tgaccgaccc ggactgggaa ccgatcatga agaaagcatc
    17581 tgccatcgtc accaaccgtg gcggtcgtac ctgtcacgcg gcgatcatcg ctcgtgaact
    17641 gggcattccg gcggtagtgg gctgtggaga tgcaacagaa cggatgaaag acggtgagaa
    17701 cgtcactgtt tcttgtgccg aaggtgatac cggttacgtc tatgcggagt tgctggaatt
    17761 tagcgtgaaa agctccagcg tagaaacgat gccggatctg ccgttgaaag tgatgatgaa
    17821 cgtcggtaac ccggaccgtg ctttcgactt cgcctgcctg ccgagcgaag gcgtgggcct
    17881 tgcgcgtctg gaatttatca tcaaccgtat gattggcgtc cacccacgcg cactgcttga
    17941 gtttgacgat caggaaccgc agttgcaaaa cgaaatccgc gagatgatga aaggttttga
    18001 ttctccgcgt gaattttacg ttggtcgtct gactgaaggg atcgcgacgc tgggtgccgc
    18061 gttttatccg aagcgcgtca ttgtccgtct ctctgatttt aaatcgaacg aatatgccaa
    18121 cctggtcggt ggtgagcgtt acgagccaga tgaagagaac ccgatgctcg gcttccgtgg
    18181 cgcgggccgc tatgtttccg acagcttccg cgactgcttc gcactggagt gtgaagcagt
    18241 gaaacgtgtg cgcaacgaca tggggctgac caacgttgag atcatgatcc cgttcgtgcg
    18301 taccgtagat caggcgaaag cggtggttga agaactggcg cgtcaggggc tgaaacgtgg
    18361 cgagaacggg ctgaaaatca tcatgatgtg tgaaatcccg tccaacgcct tgctggccga
    18421 gcagttcctc gaatatttcg acggcttctc aattggctca aacgatatga cgcagctggc
    18481 gctcggtctg gaccgtgact ccggcgtggt gtctgaattg ttcgatgagc gcaacgatgc
    18541 ggtgaaagca ctgctgtcga tggctatccg tgccgcgaag aaacagggca aatatgtcgg
    18601 gatttgcggt cagggtccgt ccgaccacga agactttgcc gcatggttga tggaagaggg
    18661 gatcgatagc ctgtctctga acccggacac cgtggtgcaa acctggttaa gcctggctga
    18721 actgaagaaa taaaataaat ccccggcggc gttcagtcgc cggggttatg tgatccccga
    18781 agatgaaact tattcaatct cttcacagac atcctgcgtt aatcgccgta taatatcttt
    18841 tcttaacaaa aacttttgta ttttatctga ggcagtgcgc ggtagttttt cgattaccac
    18901 gatatgttca ggatatttat attttgcgac ccgtttacgg ctaaaaaaag ccgccatctc
    18961 ttccagcgat aatgaatgat gcggcgcttt cagcacgaca taagcgcatg atcgttcacc
    19021 taaacgttca tcgggcattg caaccacaca ggcatcgtga attttaggat gctgcaataa
    19081 aatatcttcc acttcacggc tgctaatatt ttcgccgccg cggacaataa tatctttttt
    19141 gcgtccggta atttttatat agccagcctc atccatacgg cagagatcgc cgctgtaata
    19201 ccagccttct tcatccagcg cacgggcggt taattcaggt tcatcaaaat accccataaa
    19261 cacattgggg ccacgcgatg cttcttcacc ttcgcaacct ggcggtaagg tcttgcgtgc
    19321 gtcatcgacc actttaatct ctacacctgc ggcagcgtaa ccatcggtat gcataaagcg
    19381 cgacaaagaa ttatcgagat tcaccaccgc atgcggcgaa ctttctgtgg aaccataaac
    19441 acttaataat ttaatgctgc gctgctggca ttcgcgcgcc acttttttgg ggattgtggt
    19501 accgccgcaa agaaagaagc gcagcgctga aaggtctgcg ggttgtttct ctagtaaatt
    19561 caaaagatca tagacaaacg gcgttgcgcc tagcatacag gtgcaacgct gctgctcaag
    19621 cagcgcgaga cacgcatcag gagtgaaaat atctaacaac acgctgcgag cgccaattaa
    19681 gaatggtgcc gttacgccat gcagaaagcc cgttgcgtga ccaagtggcg caggcatcat
    19741 aaagacatcc agccaggtca gattcagccg cgcgcaataa gcccgctcac tggcgagaat
    19801 attgttatgc gttagcatca cgccctttgg cagaccctcg gttccggagg taaacagcac
    19861 cgcagctaat tcatcgccgt gggtcgttat cgccgtggtc agtgggatat tgtcggcgat
    19921 aatctgactt aatgagaggg aagaggtggc gggagccagt ttgtccacgc cgacaatttg
    19981 ttgtagttgt ggaagctgat tttgcagcgg caggattaaa tctaccggac gcgtttgttt
    20041 aaacaacgtc ggtgcaaaga acatttttgc ctgacactta ttgagcaccc acaccagttc
    20101 tgcttcccgc caggaaggca acagcggcac ggaaaccgca ccgattttca ggcaggcaag
    20161 atagataacg gtaaattcac accagccagg cagttgaaat gcgatgcgat cgcctgattc
    20221 aatacccttc gctaacatcc agtttgccag acagctcgcg gcgtgatcga gcgcgctata
    20281 ggtgtacgat gcaccatgat tatcgaccac ggcaattttg tctggcatcg cacgagcggt
    20341 ctgctgccag taatcggcca gtgaagcatc gccccatagc ccttgctgac gatacgccgc
    20401 acgacgttgt tcgttaaacg ttaatgtcac tttcattttg gactctcgaa acagaacatc
    20461 aggcccgagt tgcgggcctg tgggatgcat gattagccgt agcgaaaatc aataccgaag
    20521 gtgcctgcgg gatattgcca ctgttcgaga atagtgttac cgcacagcac ccgacaggtg
    20581 ccgcactcca gacaaccggc gggatcaaaa tgaatgtttc ctacgtcatc ctgcttataa
    20641 agtccggcag ggcaggcttt cattaattta cggaattcat tgatatcggg attttccgtc
    20701 agaatgatat gcgggtggcc ctcatcaaca tggaatttat tgacgcctaa tttgatgtcg
    20761 acgttaaccg tagcgttctg gctcatagcg cggttgctcc cttaatgcca tctttcagca
    20821 agttgatcag cccaattttc ttcgcgtgtc ccatgatcat tttgcgtacc ggctggtttg
    20881 gtttgccgtc aatggtgaac atctcgttca tgatgtcggc taccattcgt gggtattggc
    20941 taaacaggcg cgggttttcc atcagcgccg ggatcttgcg aaaatgctgc atatcacgca
    21001 tgacgcagct ttgttccagc tcacgtttgt attgcgccag actgttggcg gagaaatccg
    21061 cgcgttcttt ggcggcgatc accgttgtgg cggcagcctg agccgatgca atggctaaat
    21121 ccatgccgcg gaccgtaaaa cccaaattca ggcagaagcc tgcggcgtca ccaacgatca
    21181 tcacgccctc gttaaccagt tgcggcacca ttgccagacc gccttccggc accatatgcg
    21241 cggaatattc aagcagtttg ccgccgctaa tcagcgggcg aatggcgggg tgttgtttaa
    21301 aatcttccag catttgcggc acgctttttt gcgcatgggc gatatcaccc aatccacaaa
    21361 ccagccccaa ggatatggaa tccttgttgg tatagagaaa tcccccgccc atcaggccgt
    21421 cagaagggga accggcaaac agccaggcgg caccttcctc gcccgtaata ttaaagcgat
    21481 cgttgatctg ttctggtgtg aggccaataa cctctttaac accaacagcg taatgatgcg
    21541 gatcggaagc gggaaccatt cccagcgagc ggccaagcat cgagttaacg ccatcagcca
    21601 gaatcaccac attcgcttcg agaatatcat ccccagcctg cacgccagtg accttgtttc
    21661 cttcacgaac cagcgcgtcg acgcgaacgc ctgggataaa ctgcgcgccc gcctgctcgg
    21721 cttgttccat caaccacggg tccagacgat tacgcaatac ggtataagat gcgtgttgag
    21781 gaacatctgg ttgctcgcgg tgaaaatcga gggtaacggc gctttcttcg gttaagaagg
    21841 agattttctc gcgtgtgacc ttgcgttcta ccggcgctga tactgcaaag cctggaatga
    21901 ttgcttcaag tgtgtgggca taaagacgcc cgccggtcat gtttttacat ccggcactgt
    21961 cgccgcgttc tatcaccagg acatccagcc cggctcgtgc catgaccagt gcggcaacgc
    22021 taccagcaac gcccgcaccg accacaatgg catcaaattt gtcatccgac atgcgttatg
    22081 ctcccttcct gctgcgttta cagcgggaga tgtggttgaa aggggaatta gcggctcaac
    22141 tggctaatca gggcagggac gactttgtag atatcgccca ccagaccgta gtcggcatag
    22201 ttgaagattg gcgcattttt atctttattg atggcgacaa tcacttttac gccgttgccg
    22261 ccaaccatat gttggatctg cccggagatc cccagcgtca ggtagagatc ggatttcagc
    22321 aacacgccgg agacaccgat ataacgttca cgctccatcc agttctcgcc ttcggcaatt
    22381 ggacgtgaac agccgacttc agcattcagc accgccgcca gttcgtggac cattttcagg
    22441 tcatcctgcg ccgccagacc acgaccgacg ccaaccacac gttttgcttt gctcaggtcc
    22501 acgctgcttg cggctttcgc acggcgttcg cgacagagaa tttcatgacg cggagcaaca
    22561 taaggtaccg tttctgtcgg gcactgatga gaggtatcac tggtgcacgg ttcctgaaca
    22621 ccgggtgcaa gggtaatgat cgccagcggg ctgttgatct tttcctaagc gaacgctaac
    22681 ccgccataca tccggtgttc ggcgcaaatg tgaccatcga caatatccac cgccgtggca
    22741 tcgttcacca gcgccgcatt cagttgcaca cttaaccgtg ctgccagagc tttaccacgt
    22801 ttcgtcgcgg ccaacagcaa catagcgggg tgtttatctt tcagcagggc agcaatgctt
    22861 tcggcgtaat tttcagtgcg ttgcagcgcg tcgttttgct caagaacata aatacatttt
    22921 ggaccataag gcataactgc ctgcgcctgg tcggtatttt gtacaatggc atacacctgt
    22981 tggccccatt gctgagcgcc gccaaacagt tcagcataac gttcaggatt atcgctaaag
    23041 acccagacgc tgtttaattg actcatcgtt tcttctccgt aggcttcagt tcagggcttt
    23101 cttcagatgt tccgccagct cggcaatggc ctccggcgaa tcgttatcga gaatgatgtg
    23161 cttacgttct gtttgcggcg gtacgcgaat gccgaccagt tcagcaagtg gcgcgctctg
    23221 gctccagtca atatcacttg cctgccactg atttaccggt tttttacccg cgccgagaat
    23281 ggctttcatc gaaggaatgc gtggcacgtt aatatcggag gtgacgcaga gcacggctgg
    23341 aacagagagt tcaataactt caacatcatc ttcaagcgtg cgttcaatca ccagtgtatt
    23401 gccctgacgc tgaatagcac tcactgcatt aatcaccgga agttgcagaa tttctccgac
    23461 cagcaagcca acctgctggg cataaaggtc gccggaacct tcaccaaaga tcagtaaatc
    23521 gaagccgatc ttttcaattg ctgccgccag cgcttttgcg gtatcgagag gcagtgcatg
    23581 ttcaagttgc gcatcctgca ccaaatacag gctgtgcggc ccgcgggata gcacgtcttt
    23641 gcgcactttc gagttctgca acaatgagcc gccaacggtc agcgcggcta tctcatcgtc
    23701 atctgttgcg agctggcttg cagcttcaat ggcattgaga tcgaactggc tgattttggc
    23761 gtcggcattg tcgaaattca gggtgtattc tggagtgaca acaatgtcct gttcttcagg
    23821 caccagctta aagcaggtta ttattttcat gatttctcct gtgagtcgca tacggaacca
    23881 tctccgatgg aaggtaaaaa tggcctgaaa atagtgtgcg atagcggtgc ggggggttcg
    23941 acggagcaag ctgttttcct tgccaacaat cttgcaaaaa aaatgcgatt gctaaaacaa
    24001 aagtgcaatt tgcggcaata tagttattcc atgaccgtac aatcttgcat tcagtgtaaa
    24061 taatcttgtt tagctctcgc taaacgcggt tgtataaccg caatacacgc cgtattttat
    24121 ccggcatata ttgtttttgc ttgctcatgg aatagggagg ttgtcatgta tcaacgctgt
    24181 tttgataatg ccagtgaaac gctgtttgtc gccggtaaaa cgccacggct ttcacgtttt
    24241 gcatttagcg atgatccaaa atgggagtct ggacatcacg ttcatgacaa tgaaaccgag
    24301 ctgatttacg tcaagaaagg ggttgcaagg tttaccatcg attcttcgtt atatgtcgca
    24361 catgcggatg acattgtggt gatagaacgc ggcaggctgc atgcggtggc ctctgacgtt
    24421 aacgatccgg caacgacgtg tacctgtgcg ctgtacggct ttcagtttca gggggctgag
    24481 gaaaatcagt tactgcaacc gcattcttgt ccggtaattg ccgcggggca gggaaaagaa
    24541 gtcataaaaa ccttatttaa tgagctaagt gtgattttgc cgcaaagtaa aaatagccaa
    24601 acatcttcgt tatgggatgc atttgcctat acattagca
//
LOCUS       DAOFMX010000061        24365 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.061, whole genome shotgun sequence.
ACCESSION   DAOFMX010000061 DAOFMX010000000
VERSION     DAOFMX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.061"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            91..270
                     /gene="yjbD"
                     /locus_tag="QBA20_002787"
                     /pseudo
     CDS             91..270
                     /gene="yjbD"
                     /locus_tag="QBA20_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
     gene            complement(403..1275)
                     /gene="rluF"
                     /locus_tag="QBA20_002788"
     CDS             complement(403..1275)
                     /gene="rluF"
                     /locus_tag="QBA20_002788"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDQ6293258.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1623..2570
                     /locus_tag="QBA20_002789"
     CDS             1623..2570
                     /locus_tag="QBA20_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDQ6293259.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2640..3446
                     /locus_tag="QBA20_002790"
     CDS             2640..3446
                     /locus_tag="QBA20_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ6293260.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3456..3863
                     /locus_tag="QBA20_002791"
                     /pseudo
     CDS             3456..3863
                     /locus_tag="QBA20_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3863..4357
                     /locus_tag="QBA20_002792"
     CDS             3863..4357
                     /locus_tag="QBA20_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDQ6293261.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4423..5220
                     /locus_tag="QBA20_002793"
     CDS             4423..5220
                     /locus_tag="QBA20_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDQ6293262.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5231..6019
                     /locus_tag="QBA20_002794"
     CDS             5231..6019
                     /locus_tag="QBA20_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDQ6293263.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6071..7300
                     /gene="sorE"
                     /locus_tag="QBA20_002795"
     CDS             6071..7300
                     /gene="sorE"
                     /locus_tag="QBA20_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HDQ6293264.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7682..8371
                     /gene="pepE"
                     /locus_tag="QBA20_002796"
     CDS             7682..8371
                     /gene="pepE"
                     /locus_tag="QBA20_002796"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDQ6293265.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8462..10093)
                     /locus_tag="QBA20_002797"
     CDS             complement(8462..10093)
                     /locus_tag="QBA20_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDQ6293266.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10313..13996)
                     /gene="metH"
                     /locus_tag="QBA20_002798"
     CDS             complement(10313..13996)
                     /gene="metH"
                     /locus_tag="QBA20_002798"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDQ6293267.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14196..15020
                     /gene="iclR"
                     /locus_tag="QBA20_002799"
     CDS             14196..15020
                     /gene="iclR"
                     /locus_tag="QBA20_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDQ6293268.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15337..17521
                     /gene="espL4"
                     /locus_tag="QBA20_002800"
                     /pseudo
     CDS             15337..17521
                     /gene="espL4"
                     /locus_tag="QBA20_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17490..19227)
                     /gene="aceK"
                     /locus_tag="QBA20_002801"
                     /pseudo
     CDS             complement(17490..19227)
                     /gene="aceK"
                     /locus_tag="QBA20_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19411..20715)
                     /gene="aceA"
                     /locus_tag="QBA20_002802"
     CDS             complement(19411..20715)
                     /gene="aceA"
                     /locus_tag="QBA20_002802"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDQ6293269.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20745..22346)
                     /gene="aceB"
                     /locus_tag="QBA20_002803"
                     /pseudo
     CDS             complement(20745..22346)
                     /gene="aceB"
                     /locus_tag="QBA20_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22615..23544)
                     /gene="metA"
                     /locus_tag="QBA20_002804"
     CDS             complement(22615..23544)
                     /gene="metA"
                     /locus_tag="QBA20_002804"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDQ6293270.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23701..24144
                     /locus_tag="QBA20_002805"
     CDS             23701..24144
                     /locus_tag="QBA20_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDQ6293271.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            complement(24259..>24365)
                     /gene="rrf"
                     /locus_tag="QBA20_002806"
     rRNA            complement(24259..>24365)
                     /gene="rrf"
                     /locus_tag="QBA20_002806"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6005 a         6227 c         6182 g         5951 t
ORIGIN      
        1 gttatcgctc gccacgggaa tatctgcggc agcgggcttg taatgggtta agtgataaca
       61 gatgtctgga aatatagggg caaatccagt cgcgtactga ccaactccga aaccaacagc
      121 attaagaaag agtacatcga taaattaatg gtcgagcgtg aggctgaagc gaaaaaagcc
      181 cgccaattga agaaaaagca ggcttataaa ccggatcctg aagcgtcatt tagctggtca
      241 gcaaatacgt cgacgcgcgg caggcgctaa tgaacaatca acgcctgaga ttttcactcg
      301 tctgtgtgga cacaatcttc ccctcaccct aaccctctcc ccgaaggggc gaggggactg
      361 tccgagctct ttttggacgt tttcatcagt ctgaggcgtt aatcagcgtc ccttcttttt
      421 acgccccggc gaggtaaaac gcttaccgtt ggacgccggg cgaccgcctt tttccgccgt
      481 tttttccatc ttaacgactg gacgtttgat gcccgctgtt ttcggtttgg ccttcgcttt
      541 aggttttgcc tcggaagagg aattttcgat gagcttaaag aggtcgatta actcatcgtc
      601 ggttaaatcg cgccactccc ccagcggaat gccgcttaag ctgacgttca taatgcgcgt
      661 acgttccagc tttttcactt catagccgaa atgctcgcac atgcgccgga tctgacggtt
      721 cagcccctgc actagggtaa tgcggaagac aaacggcgct tcttttttga ctttgcactt
      781 tttcgtcacc gtcccgagga tcggcacccc cgcccccata ccacgaataa attcatcggt
      841 aatcggttta tcgaccgtca ccagatactc tttctcatga tcgttacccg cacggaggat
      901 cttattcacc agatcgccgt gattggtgag gaaaatcagc ccctgggagt ctttatccag
      961 gcggccaatc gggaacacgc gtttgctgtg gttaacgaaa tcgacaatgt tatcgcgctc
     1021 gccatcttcg gtggtgctga caataccgac gggcttgttc agggcgataa gtaccaaatc
     1081 ttcggcttcc cgaggctcaa tcaactgacc gtttactttc acaatgtcgc cgggtttcac
     1141 ctgatcgcca atggtggctc gcttgccatt aaggaacaca ttgccttgct cgatatagcg
     1201 atccgcttcg cggcgtgagc aaattccgct ttcgctgatg tatttattta aacggactga
     1261 tgagtcgggc agcataaatt ctcctgtaaa acgcggaata taccgtacct cttagcgggg
     1321 aaaaacaggg ttatgcggca acgtcgaaat taaatgtgat ctgccacgcc ttttatcaca
     1381 aaagtgtctc cggaagtacg atgcaaattt gcctggcaaa ataaggataa tcaacatatt
     1441 acgaatcgct gcacacctgt gctgatgact actacgcaga gaaacaaatg tgcaagagaa
     1501 agcgctattt ctccccatgc aaagagtgaa aaatcgcttt gaaaggccct catgatgggg
     1561 ttatatcaga tcaagcggtg agaaagcgta tttgcacaaa tgtgcagaac caggaggcgg
     1621 caatggaaaa cagtgacgat atccgtttga ttgtgaagat tgcccaactc tattacgaac
     1681 aggatatgac gcaggcgcaa atcgcgcgcg aactgggtat ttaccgcacc accatcagcc
     1741 gcttgcttaa acgaggccgc gatcagggaa tggtcaccat cgccatcaac tatgactaca
     1801 acgaaaatct ctggctggag cagcaactga agcaaaagtt tggcctgaaa gacgttgtgg
     1861 tggtgtcggg aaatgatgag gatgaagaga ctcaactggc gatgatgggg ttacacggcg
     1921 cgcaactgct ggatcgcttg ctggagcctg gcgatattgt cggtttttcc tggggtcgcg
     1981 cggtgagcgc actggttgaa aacttgccgc aggcggggca atcgcggcag ttaatctgcg
     2041 taccaattat tggcggaccg tccggtaaac tcgaaagccg ctatcatgta aacacattaa
     2101 cctacagcgc ggcagcgaag ctgaaagggg aatcgcatct cgcggatttt ccggctctgc
     2161 tggataaccc attaattcgt aatgggatca tgcagtctca gcactttaaa accatctctg
     2221 cctactggga taatctggat atcgccctgg tgggaattgg ctcaccggcc attcgcgacg
     2281 gcgctaactg gcatgcgttt tatggtggtg aagagagtga cgacctgaat gcccgccagg
     2341 ttgctggcga tatttgctcg cgcttttttg atattcacgg cgcaatggtt gaaacgaata
     2401 tgagcgaaaa aacgctctct atcgaaatga ataaattaaa gcaggcgcga tattccattg
     2461 gcattgccat gagtgaagaa aaatacagcg gaattattgg tgcactgcgt ggaaaatata
     2521 ttaattgtct ggtaacgaat agcagcacag ctgaactatt actgaaataa aaatcttccc
     2581 gatgtttacc gtaataaaaa ttacggcggg tccctattat ctaaaatcag gagtctgtta
     2641 tgcaaacgtg gttaaatttg caggataaaa tcattattgt caccggcggc gcatccggta
     2701 ttggtctggc gattgtagag gaattattag cacaaggcgc gaatgtacag atggtcgata
     2761 ttcacggtgg cgatggtcaa tatgaaggtc ataaaggcta tcagttttgg ccgaccgata
     2821 tttctagcac aaaagaggta aatcatacgg tagcggaaat tatccagcgt tttggtcgca
     2881 tcgacggtct ggtcaataac gccggggtca atttcccgcg tctgctggtc gatgagaaag
     2941 cgcctgccgg gcagtatgaa ctcaacgaag ctgcattcga aaaaatggtc aatatcaacc
     3001 agaaaggtgt ttttctgatg tcgcaggcgg tggcgcgaca gatggtcaaa caacatgatg
     3061 gcgtgattgt gaatgtttcc tcagaaagtg ggctggaagg ctcagaagga caaagctgtt
     3121 acgccgctac caaagccgcg ctcaatagct tcacgcgctc ctggtcgaaa gagctgggta
     3181 aacacggtat ccgtgtggtc ggtatcgcgc ccgggattct ggaaaaaacc gggctacgca
     3241 cgccggaata tgaagaggcg ctggcgtgga cgcgcaatat caccgtcgag cagctgcgtg
     3301 aaggctatac caaaaacgcc attcctattg ggcgcgccgg aagattagca gaagtagctg
     3361 attttgtttg ttatctgttg tctgaacgcg ccagctacat caccggagta accactaaca
     3421 ttgcgggcgg caaaacgcgc ggctaaggag gctttatggt caacgccatt ttttgtgccc
     3481 acggcaaatt agcttgcgcc atgctggaat cggttcagat ggtttacggc aacgccaatg
     3541 ttgaagccgt tgcatttgtg cccggtgaaa acgccggaga cattgcggta aagctggaaa
     3601 agttagtaag cattcacaat caagatgagt ggctgattgc cgtggattta cagtgcggca
     3661 gcccgtagaa tgcggcagca atgcttgcga tgcgcaatcc tcgtttgcgg gtaatcagcg
     3721 gcctttctct gccgctggcg ctcgaactgg ttgataacca ggacagcatg aatgttgatg
     3781 aactgtgtga acacctgacg caaatcgcaa agcaaacctg cgttgtctgg aaacaactgg
     3841 caacgacaga ggaggatttc tgatgaacat tacgctcgcc cgcattgatg accgtctgat
     3901 ccacggccag gtcactaccg tctggtcgaa agtggctaac gcccagcgca ttattatctg
     3961 caatgacgaa gtttataacg atgaagtgcg ccggacatta ttgcgtcagg ctgcgccacc
     4021 gggaatgaag gttaacgtgg tcaatattga aaaagccgtc gcggtttatc acaacccgca
     4081 atatcaggac gaaaccgttt tttatctttt cactcgccca caggatgcct tagcgatggt
     4141 tcgccagggg gtaaaaatag gcaccctgaa tattggcggc atggcctggc ggcccggtaa
     4201 aaaacagtta accaaagcgg tttcattaga tgatgatgat attaacgcat ttcatgagtt
     4261 aaataatctt ggtgtcatac tggatttacg cgtcgtagcg tcagatccct caatcaatat
     4321 tatcgacaaa ataaacgaac agttaatcgc aaattaaata atagcgcctg tgtacatatg
     4381 ctttaacagg cagggatgac gacacttaaa aggtgacaaa tcatggaaat aagtacccta
     4441 caaatcattg ctatatttct tttttcctgt attgccggaa tgggcagcgt gctggatgaa
     4501 tttcagactc atcgcccgtt aattgcctgt acggtgattg gtttaattct cggtgattta
     4561 aaaaccggaa taatactcgg cggtacgctg gaattgatag ctctctgctg gatgaacgtc
     4621 ggcgcggcgc aatctccgga ttctgcactc gccagcataa tctccgccat tctggttatc
     4681 gttggtcagc aaagcatcgc caccggaatc gccatcgcgt tgcctgtggc tgcggcaggc
     4741 caggtgctga cggtgtttgc ccgtaccatc accgtagtgt tccagcacgc ggcggataaa
     4801 gcagcagaag aggcgcggtt tcgcactctc gatattctgc atgtctccgc gcttggcgtg
     4861 caggcgctgc gcgtcgccat tccggcactg attgtctcac tgttcgtcag cgccgatatg
     4921 gtcagcaata tgttaaacgc cattcccgaa tttgtgaccc gaggactaca gattgctggc
     4981 ggttttatcg tggtggtcgg ttacgccatg gtgctacgta tgatgggcgt gaaatatttg
     5041 atgcctttct ttttcctcgg tttcctcgca ggtggctacc tcgatctcag cctgctggcg
     5101 ttcggtggcg tcggcgtgat catggccctg gtctacatcc agctaaatcc acagtggcgt
     5161 aaagctgaac ctcaactcca ggccaccgcc tccaccgccc ttgaccaact tgacgattaa
     5221 cggagccatc atggaacaga gaaaaattac acgcagcgat ctggtgagca tgtttctgcg
     5281 ctccaacctg caacaggcgt cctttaactt tgaacgtatt cacgggctgg gcttttgcta
     5341 cgacatgatc cccgccatca agcgacttta cccattaaaa gaggatcagg ttgcggcgct
     5401 caggctacac ctggtgttct tcaataccac gccagccgta tgtggcccgg tcatcggcgc
     5461 ggaaattgat gacggtacca tcaacggcat caaagtcggt ctgatgggac cattggcagg
     5521 agttggcgat ccactggtct ggggaacgct gcgcccgatt accgccgcgc tcggcgcatc
     5581 tctggcactt tcgggcaaca ttctcggccc gctgctgttc ttctttattt tcaacgcggt
     5641 gcgtctggcg atgaagtggt atggcctaca gctcggcttt cgcaaagggg tgaatatcgt
     5701 cagcgatatg ggcgggaatg tgctgcaaaa actcaccgaa ggcgcgtcga ttctcggact
     5761 gtttgtgatg ggcgtgctgg tgaccaaatg gacgtcaatc aacgtaccgt tggtggtttc
     5821 acaaacgcat gccgccgatg gctccaccgt caccatgacc gtgcagaaca ttctcgacca
     5881 actttgccct ggtttgctgg cgctcggtct gacgctacta atggttcgtc tgctcaacaa
     5941 aaaaattaac ccggtatggc tgattttcgc cctgtttggc ttagggatta tcggcaatgc
     6001 tctgggcttc ctgtcctgat tcttcgcccc ggcacgactg ccgaggccat cgctcaacat
     6061 gaggtggttt atgaaaacaa cagctctgcg tctttatggt aaacgtgatt tacgcctgga
     6121 aacctttgac cttcctgaaa tgcaggagga tgaaatcctc gcaacggtgg tcactgacag
     6181 cctgtgcctc tcttcctgga aagaggccaa tctgggtgaa aaccataaaa aagtacccga
     6241 cgatgtgacg accaacccaa tcatcatcgg ccacgaattt tgcggcgata ttctggccgt
     6301 gggtaaaaag tggcagcaca aattccagcc gggtcagcgt tatgtgattc aggccaacct
     6361 gcaactcccc gaccgcccgg attgccccgg ctactccttc ccgtgggtag acggcgaagc
     6421 gacgcatgtg gttattccca acgaggtcat ggaacaatat tgcctgctgg catacgacgg
     6481 cgaaacctat tttgaaggct cactggttga accgctttcc tgcgtgattg gcgcgttcaa
     6541 cgccaactat catcttcagg aaggtagtta taaccacacg atggggattc gcccgcaagg
     6601 gcgcacgctg atcctcggcg gcaccggacc aatgggactg ttggcgattg attatgcgct
     6661 gcatggacca gttaacccgt cgctgctcgt cattaccgat accgacaacg acaaattgag
     6721 ttatgcgcgc aagcactatc catcagaacc gcaaacactg attcattatc tcaatgccac
     6781 cgatgcagca tttgatacgt taatggcgct gagtggcggt cacggcttcg atgatatttt
     6841 cgtctttgtg cctaatgaag gactggtgac tctcgcctct tccttgctgg cgacagatgg
     6901 ttgcctgaat ttcttcgccg gaccgcagga taaacatttc agcgcgccaa ttaatttcta
     6961 cgatgtgcat tatgcattta cccactacgt gggcacgtca ggcggcaata ccgacgacat
     7021 gcgcgcggcg gtcaaactga tcgaagagaa aaaagtgcag gccgcaaaag tggtaacaca
     7081 tattcttggg ctgaatgccg cgggcgaaac cacgcttgaa ttgcctgccg tcggcggcgg
     7141 caaaaagctg gtgtataccg ggaaatacct gccgctgacg tcactcacgc agattcagga
     7201 tcaagcactg gcggcgattc tggcgcgtca tcaggggatc tggtccggtg aggcggagca
     7261 atacctgctc gcccatgcag aggcaatttc ccatgattaa tcgcgctact taactctgca
     7321 tgtcgctggc tggtcgcccc agcaacttcg cgaccggatg tgcggctgat tgctgaagca
     7381 gcacaatttg cacgagaagg ataaaaaata tcagcgcctt aaggggcgct caaattggtg
     7441 gcaaagtaca cattgtttat gccggatgcg gaatgaagcc ttattcggtc tacataattt
     7501 tgaaaatcca atatattgca gttatctggt aggccaggta agcgtagcgc atcaggcaat
     7561 gctgtgtttg tcatcagtct cagcgcccgg tgcaatacgt gacatcgtga gccatgtcag
     7621 taagtggtta aattcgcagt ggtagcatgc cttccgtgat ttcttactaa ccggagaagt
     7681 aatggaactg cttttattga gtaactcgac gctgccgggt aaagcctggc tggaacatgc
     7741 actgccgcta attgctgaac agttgcaggg tcgccgctca gcggtgttta tccctttcgc
     7801 tggcgtaacg cagacctggg atgattacac agagaaaacg gctgcggttc tcgctccgct
     7861 gggtgtttct gtcaccggta ttcatagcgt tgtcgatccc gttgccgcga ttgaaaatgc
     7921 ggaaattgtg attgtcggcg gcggtaatac tttccagttg ctgaaacagt gccgcgagcg
     7981 cgggctgctg gctccaatta ctgacgtggt taaacgtggc gcgctgtata ttggctggag
     8041 cgcaggcgct aaccttgctt gcccaaccat tcgtaccacc aacgatatgc cgattgtcga
     8101 tccgcaaggt ttcgatgcgc taaatctgtt cccgttgcaa atcaacccgc acttcaccaa
     8161 cgcgctgccg gaaggccata aaggtgaaac ccgtgagcag cgtattcgcg aactgctggt
     8221 cgtcgcgcca gaactgacga ttattggtct gccggaaggt aactggatca cagtgagtaa
     8281 aggtcacgct acgctgggtg gcccgaacac cacttatgtg tttaaggctg gtgaagaagc
     8341 ggttccgctg gaagctggtc accgttttta agtgaattgc cggatgcatg atccggcatc
     8401 ttgttcatag tgcctgatgc gacgcttgac gcgtcttatc aggcctacag gattcacaat
     8461 attaatactc gtcccgtcct tcatcttcat ccggctgttc cagcacactg taagccaccg
     8521 aacaaaacag cgagttcagg cgctgcatat ctcccagtaa gcctaaatgt agcgaactgg
     8581 tttcaatgct ctgcacgttt tgctgatgca ggcgatcgac gtgggcgtgg gaatagcggc
     8641 gattaagaat gcgaaaacga tgtttgctgc gacgcaaacg acgagcgctg gtgacatcgc
     8701 cagagaagaa caccgacatt gccagcttta aattactgag caattgctca tagagcgcat
     8761 ccagttcctt caacccatca agcgaaaatg cccgccgtgc tgccagcgat ttatcggcaa
     8821 tttcgctgcc catgcgctcg acgatatcgg aggcctgttc aaggttgagc gacatttcga
     8881 tgatctccgc ccagcggcgc gactcttcct ccgccaactc ttcttttggc atccgcgcca
     8941 gatacagctt aatggcggtg tagagaacgt tgatatcatc tgccagctta cgcagctctt
     9001 tctcctgccg tggctcgccg tgcatcactt tattcagccc ttccatcatc tgttccatgg
     9061 cgtcgccaat gcgcagggtt tcgcgcgcgg cgttcgccag agcaagcgtg ggcgtgtcca
     9121 gcgcgctgac atccagatgt ttaggccgta gctgggtatc cagttccggt tcatcgcgaa
     9181 taatcgtttt gcaaaaccgt gccatcgggt caacaaatgg cagcatgacc aggcaacgta
     9241 caaggttgta gaagacgtgg aaatagatca ccagttccgc ttttggcaat gacagcttcc
     9301 ccattgtctc tgccagcaaa tggacaaacg gcaggataat caggctaccc accagcttaa
     9361 acagcagact acccagcgcg acacggcggg ctgcggcatt ggcggcactg ttgttgagca
     9421 tcgccagcag gccggaaccg aggttagctc caatcaccag acagagcgcc acggggaagg
     9481 agataatgcc tgcagcggtc agagtcgcgg tcagcagtac agcagcaagg ctggagtagc
     9541 tgataatggc gaacatcgcg ccaatcagcg catccagcag aatatcgccg gtcagcgagg
     9601 caaagatcac ctgaacgccg tttgcctggg tgatcggcgt tacggcctgc acaatcaact
     9661 ccagcgccag caaaatcagc ccaagaccaa taccgacgcg gcccagttgc ccggcgcgtg
     9721 actgtttgcg tccgaggaaa aaaatcacgc cgataaaaat aagtaacggt gacagccagg
     9781 ataagtcgaa ggtgagaata cgcgccatta gcgccgtccc gacatctgcc cccagcacaa
     9841 tgaccagagc cggtgcgagg gctaccagat cctgagcgac gaacgaggtc accagcatgg
     9901 tggtggcatt actgctctgt accagtgcgg taacgccgat ccccgcgcaa aaggcgagcg
     9961 gctttttttc gacgctccgg ctaaggacag tacgcaaacg cgcgccgaag acgcgcatta
    10021 cgccggttcg aacaatatga gtcccccaga ccagcagggc gacggcagaa agcaggtgaa
    10081 gcagcgttaa cacgagttca ggttctcctt atcgttatat gttcctgaag caattgaatg
    10141 accaaaggac cggaaacacg ctgactggca tctttactgt gccttttgac attcgttttt
    10201 ccggctaaga ttattccagc ctgtttccag tataagtgtt tcatattaat aaagagacag
    10261 ggcactcatt gagcgccctg tttgttgtaa ggaaaagtga cagatttgtg aatcagtccg
    10321 cgtcataccc cagattcggt gccagccagc gctcaacatc ggagacgctc atacctttac
    10381 ggcgggcata gtcttcaacc tgatcgcgct gaatttgcgc caccgcgtag tacttgctgt
    10441 ccggatgact gaaataccaa cccgaaaccg atgcaccagg ccacatcgcg aaagattctg
    10501 tgagtttcat gccagtatgt ttttccactt ccagcagctc ccagatggtg gctttttccg
    10561 tatgttccgg gcaggccgga tagcccggtg ccggacggat gccctggtag ttttcgcgga
    10621 tcagttcttc gttgctgagg ttctcgttcg gcgcatagcc ccagtagact ttacgcacac
    10681 gctcatggag atactccgca aaggcttcgg ctaaacggtc ggcaagcgct ttcaccatga
    10741 ttttgttgta atcatcgtgc tgcgcttcaa aggcatcagc cagtgcgtcc tcttccagcc
    10801 cgccagtcac ggcaaatgcg ccgatgtaat ctgctttacc agaaagcttc ggcgcaacga
    10861 agtcagcgag acagtagtta gcgaatcccg ttttttcggt ctgttgacgc agatggtggc
    10921 tgacgttgat cacatgggtg cgcgtttcgt cacggtagat ttcaatgtca tcgcccacac
    10981 ggtttgccgg gaacaggccc accacgccac gcggattcag cgttttctcg gcgcttaatt
    11041 tatccagcat gtcgttggcg tctttaaaca gccgctgcgc ctcaacgccc accacttcat
    11101 cttccagaat gcgcggatac ttcccggcca gcgaccaggt cataaagaac ggtgtccagt
    11161 cgatgtaatt acgcagcgtt tcgatgctgg cttccacttc ctgcacgccg agacggtgcg
    11221 ccaccggcgg cgtgtaagcc tgccagtcaa atgcgaaatc gttatcgcgc gccgcttcca
    11281 gcgtgaccgg tggtgtgcgt ggtttcttgc gcccgtgctg aatacgcacg gtttcgtact
    11341 ccttgcaggt acgagcgaca aaatcatcac gctgagtatc ggaaagcagc gccgctacca
    11401 caccaacggt gcgcgaggca ttctgcacat acaccgtagg gccgctgtag ttctgctcga
    11461 ttttcaccgc cgtgtgcgct tttgaggtgg tcgcgccgcc aatcagtaac ggaatggcga
    11521 agccctgacg ctccatctct ttcgccacgt taaccatctc gtccagcgac ggcgtgataa
    11581 gccccgaaag gccaatcaaa tcagcattca cttctttggc gatacggaga attttttccg
    11641 caggcaccat aacgccgaga tcgacaattt cgtagttgtt acattgcagc accacaccaa
    11701 cgatattttt accgatgtcg tggacatcgc ctttcacggt ggcgatcacc atcttgccgt
    11761 tggttttacc ctgctctttg ctggcttcaa taaacggttc gagatacgcc actgcctgtt
    11821 tcatgacgcg cgccgatttg accacctgtg gcaggaacat tttgccttcg ccaaacagat
    11881 cgccgaccac gttcatgccg tccatcaacg ggccttcaat cacttcaatc gggcgcgtag
    11941 cctgctggcg ggcttcttca gtatcctgct cgataaactc ggttatgcct ttgaccagcg
    12001 agtattccag acgtttattc acgtcccacg agcgccactc cgcttgctgg gcgttagcag
    12061 tgtcgtccgc tttactgccg cgatatttct cggcaagctc cagtaaacgc tcagtgccat
    12121 cgtcacgacg attaagaatc acatcttcca ccgcgtcgcg cagttcagcg ggtaggtcgt
    12181 cgtaaatcgc cagttgcccg gcgttgacga tccccatatc catgccattg cgaatagcgt
    12241 agtagaggaa cactgcgtga atggcttcgc gcaccggatc gttgccacgg aatgagaaag
    12301 aaacgttaga tacgccgccg gaaatcagcg cgtgcggcag ttcgcgtttg atgtcttcac
    12361 acgcgccgat aaagtcctgc gcgtagttgt tgtgctcttc aataccggtg gcgaccgcga
    12421 agatgttcgg gtcgaagatg atatcttctg gcgggaagcc aacctcttcg gtgaggattt
    12481 tgtacgcccg acggcaaatc tcgattttcc gtgcgcgagt atcggcctgt ccctgttcgt
    12541 caaaggccat caccaccact gccgcaccgt agcgacgcaa caatttcgcg tgatggataa
    12601 aggcgtcgac gccctctttc atcgagatag agttaacaat gcctttgccc tggatacact
    12661 tcagaccttt ttcaatgaca tcccattttg aggagtcgat cataatcggc acgcgagcga
    12721 tatccggttc accggcaatc agattgagaa aacgcaccat cgccgcttcg gcatcgagca
    12781 tcccttcatc catgttgata tcgataatct gcgcgccgtt ttccacctgc tgacgcgcca
    12841 catccagcgc ctcgctgtat ttctcttctt tgatcaggcg cttgaactta gcagagccgg
    12901 tgacgttggt gcgttcaccc acgttcacaa acaggctatc gtcgccaatg ttcagcggct
    12961 ccaggccaga caaacggcag gctacgggaa tttccggcag tttgcgcggc gctaatcctt
    13021 ctactgcacg actcatcgct gcaatatgtt gcggcgtggt cccgcagcaa ccaccgacga
    13081 tattgagaaa gcccgcttgc gcccattcac gtatctgttt tgccatcgtg tcggcgtcga
    13141 gatcgtattc accaaaggcg ttaggcagcc cggcgttcgg gtgggcggtg acgtagcatt
    13201 ccgcaatccg tgatagttcc tgcacgtact ggcgcagttc atcgggcccc agcgcacagt
    13261 tcaggccaaa ggtcagcgct tcggcgtggc gcaatgagtt gtaaaatgct tcggtggtct
    13321 gcccggagag cgtgcgcccg gaggcgtcgg tgatggtgcc ggagatcata atcggcagct
    13381 caacgcccag cgcttcaaac tccgttttca ccgcaaagac cgccgctttg gcgttaaggg
    13441 tgtcgaaaac ggtttcaatc aggatcagat ccgcaccacc ttccaccagc gctttggtgg
    13501 attcgcgata ggccgccgcc agctggtcaa aagtgatgtt gcggaatgcc ggatcgttga
    13561 cgtccggaga aatagacgcc gtgcggttgg ttgggccaag aacaccggca acgtagcgcg
    13621 gtttctccgg cgtgcgcgcg gtccactcgt cagcacaggc gcgcgccagt ttcgccgccg
    13681 caaagttgat ttccgccgac agggattcca tctggtaatc cgccatcgca atggtcgtgg
    13741 agttgaaggt gttggtttcg atgatatccg cgcccgcttc aaagtaggcg ttgtggatag
    13801 cggcgatcac ttccggttta ctgagtacca gcaggtcgtt gttgcctttg aggtcgcatg
    13861 gccagtcggc aaagcgttca ccacgaaaat cggcttcgtt cagtcgatag ctctggatca
    13921 tggtgcccat accgccgtcc aacaccagaa tacgttcatt taactgcgca cgcagttgtt
    13981 ccactttgct gctcacactt gctcccgaca cgctcaaccc agatttaata aaaattcaac
    14041 aaaccatact ggaataaacg catctgtggt aaaagcgacc accacgcaac atgagatttg
    14101 ttcaacatta actcatcgga tcagttcagc aactattgca ttagctaaca ataaaaatga
    14161 aaataatttc cacgatacag aaaaaggagg ctgtcatggt cgcacccatt cccgcgaaac
    14221 gcggcagaaa acccgccgtt gccaccgcac cagcgactgg acaggttcag tctttaacgc
    14281 gtggcctgaa attactggag tggattgccg aatccaatgg cagcgtggca ctcacagagc
    14341 tggcgcaaca agccgggtta cccaattcca cgacccaccg cctgctaacc acgatgcaac
    14401 agcagggttt cgtgcgtcag gtcggcgaac tgggacactg ggcaatcggc gcacatgcct
    14461 ttatggtcgg cagcagcttt ctccagagcc gtaatttgtt agcaattgtt caccctatcc
    14521 tgcgcaatct aatggaagag tctggcgaaa cggtcaatat ggcggtgctt gatcaaagcg
    14581 atcacgaagc gattattatc gaccaggtac agtgtaccca cctgatgcga atgtccgcgc
    14641 ctatcggcgg taaattgccg atgcacgctt ccggtgcggg taaagccttt ttagcccaac
    14701 tgagcgaaga acaggtgacg aagctgctgc accgcaaagg gttacatgcc tatacccacg
    14761 caactctggt gtctcctgtg catttaaaag aagatctcgc ccaaacgcgc aaacggggtt
    14821 attcatttga cgatgaggaa catgcgctgg ggctacgttg ccttgcagcg tgtattttcg
    14881 atgagcaccg cgaaccgttt gccgcaattt ctatttccgg accgatttca cgtattaccg
    14941 atgatcgcgt gaccgagttt ggcgcgatgg tgattaaagc ggcgaaggaa gtgacgctgg
    15001 cgtacggtgg aatgcgctga ctttttctgg cgggcagagg caatattctg cccatcatac
    15061 ctgagtggca atagaataag ggtgtctgtt aatcgcattg acgccaaaat aacttaatgt
    15121 catacacttc acacactttt attaactcag cattattttt aaacatcaaa ccacttaatt
    15181 attaacaaaa tcgcaagatc tcattccacc gaaactttta cggctgcatt atgagattga
    15241 gcgatttatg atattttttt agttgtaatc atattaaatt ttttcactgt tctgatagtt
    15301 aaaattcaag acatcaataa acaatgagat atttaaatga ttactcgtat tcctcgtagt
    15361 tctttctctg caaatattaa taatacagcc cagacaaatg aacaccaaac cctgagtgaa
    15421 ttgttttata aagaactcga ggataaattt tctggcaagg agctggcgac gcctctatta
    15481 aaaagcttct cagagaactg tagacaaaat gttcgtcata tctttagcaa caaggatttt
    15541 gtcattaaat tttccacgtc cgtcttacaa gctgataaga aagaaattac gataattaat
    15601 aaaaacgaaa acacgacact cactcaaacc attgctacaa tatttgaaga atacctaatg
    15661 gaaattttac ctcaacgctc agacactctt gataaacaag aattaaacct aaaatcagat
    15721 agaaaagaaa aagaattccc aagaattaaa cttaatggtc aatgttattt cccggggcgc
    15781 cccaaaaccg tatagtatac cgacacattg ctgcacaata tattaatgat atttatcaga
    15841 atgttgatta caaaccccat caagatgatt actcttcagc tgaaaaattt ctcactcact
    15901 tcaacaaaaa atgcaaaaac cagactttgg cgttgatttc cagccgtcct gaggggcgtt
    15961 gcgttgctgc ctgcggtgat ttcgggctag ttatgaaagc atattttgac aagatggaat
    16021 caaatggcat cagtgttatg gcagccatat tactggtgga taaccatgct ttgacggtcc
    16081 ggctaagaat aaagaacaca actgaaggat gtacccatta cgtggtttcg gtttatgatc
    16141 ctaatgtaac taacgataaa ataagaatta tgagcgaaag caaagaggat attaaacact
    16201 attctctgat ggattttatg aatgtagatt atagcctcct gaaatggtca aatgatcatg
    16261 ttattaacca atctgttgca ataattccag cacttccgaa agaacagcta ttgatgttaa
    16321 aaggaactgt ggatgaaata acccctccat tatcaccagc aacgatgaat ttgctaatgg
    16381 caattggtca gaatcaccaa cttacgcaac tgatgattca gctccagaaa atgccagaac
    16441 tacatagaac agaaatgttg actgcctata atagtattaa cctccccggt ttatatttgg
    16501 ctataaatta tggcaatgcg gatatcgttg agactatttt caattcattg tcagaaacag
    16561 gatatgaagg gttactctcg aaaaaaaatc tcatgcatat tctggaggca aaagataaaa
    16621 atggtttttc tggattattt ttagcgatat cacgtaagga taaaaatgtt gtaacctcga
    16681 ttctgaacgt cttacctaaa ctggccacaa cacatcattt agataatgaa caggtgtata
    16741 aattcctgag tgccaaaaat agaacgtcca gccatgtttt ataccatgtt atggcgaatg
    16801 gtgatgccga catgctgaaa atttttttgg tcgcgttacc tttgttaatt cgcacatgtc
    16861 atttgactaa agaacaggta ctcgatctcc tgaaggcaaa ggatttttat ggttgcccaa
    16921 gactatacct ggcgatgcaa aatggacata gcgatatcgt gaaagttatt ctcgaagcat
    16981 tgccctgtct ggcccaggaa attaacattt cagcttccga tattgtagat cttctgaccg
    17041 ctaaaagtct tgcgcgcgac acgggtttgt ttatggccat gcagcgcgga catatgaacg
    17101 ttattaatac tatttttaac gcattaccca ctctttttaa tacgtttaaa ttcgataaaa
    17161 aaatatgaag cccctcctcc tggcaaataa ttctaatgaa tatcccggtt tgttttcagc
    17221 gatacagcat aaacaacaaa acgttgtaga gacggtttat cttgctttat ctgaccatgc
    17281 acgcctgttt ggatttaccg ctgaagatat tatggatttt tggcaacaca aagcgccaca
    17341 aaaatactct gcctttgagt tggcttttga atttggtcac cgggttattg ctgaattaat
    17401 ccttaataca ttaaataaga tggctgaaag ctttggcttt acggataacc ctcgatacat
    17461 tgcggagaaa aattatatgg aagctttact caaaaaagca tctccccata ccgtacgcta
    17521 aatctttgcc tgcgccgata cgcataaaca tcttccacat gcccttcacg tatgcggttt
    17581 tgtagtgcgc gccagtaatc agcgcggaac aggtcggcgt gcatctcttc aaacagcgga
    17641 ccaatacgcg ggtcggcgca tagccagtgg cgaaactctt ccgggaaaac atcgcctggc
    17701 gagacgctgt accacggttc gctggcaagt tcgtcttccg gatagcgcgg cggcgggatg
    17761 tcgcggaaat tcacttccgt catgtagcaa atttcatcgt aatcataaaa aaccacacgc
    17821 ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc cagggaaaat gttagcagcg
    17881 gcaagctggc gaatagcgtt accgtattct tcaatggcgt cgcgcaactg ctgaccttcc
    17941 acttgttcca gccagatatt gagcggcacc atccgccgct cgattataga gatggcgaat
    18001 tacaatctgc tcaccaagat cggtgatttt ttccgctgct tcctggagca gtaattccat
    18061 taataccggg gaaatatgcc gcttctccag cacaaagttt tcaaactcct gggtgtccgc
    18121 cattcggccc acacggtcgt gctctttcac cagttgatag caggcacgaa cgtgagcggc
    18181 agacatctct ttctgcggcg cgaacttgtc tttgatgact ttgaataccc gatcaaagcc
    18241 cggcagcgta aacaccaaca tcaccattcc acgaataccc ggcgcttcaa tgaactgctc
    18301 attacagccc tgtagataaa cgagatattc gcggtagctt tcggttttgg cgtgcttctg
    18361 gcagccgata gccatataca attcagcagt ggttttacct ggcagaattt cccgtagcca
    18421 ctcgaccagc gctgcgggca gcggtgcata aaccataaaa taagaacgcg caaagccaaa
    18481 aacaatgctc gcttcggcgg tcgtcgtcag gcaggtatca ataaataact cgccgtcgtc
    18541 cgtctggtgg atcggcagta agaagggcaa cgtgccggaa ggcgtgatca gtttgcccac
    18601 cagccaggcg gctttattgc ggtaaaacag ttcgttcgcc acctgtaaat gactttccgc
    18661 gaggttgtct gtccccagcg tttccgtcag atggcgaatg atgtaatgaa tgtcacggct
    18721 tttattctgc cagcgcaggc gcagcggcag gtcgctgata acgcgcatca gtagagattc
    18781 ccagccgtga tcggggtgaa agtctttcgc cagcgggcgg ggaatggtac gaaagcggcg
    18841 ctctggctga gagctaaaga taaaaagccg ctcgggagta agcgagcggt ggtcaaataa
    18901 ccgacagtac acggagttaa aaaagctctc cgcaatctcg aagcgcgggt aatccggcaa
    18961 cagccgcgtg taatgctctt taacgcgcag taaaaatgcc gcgtccgtac tttggccgtt
    19021 agtaatgcag cgcagttgct ccacgaccag accaacgtga tgatcgtaaa gatggatacg
    19081 gtttttcatc gcctgctgga cagcatgcca gtcggcctgt tcgaaacgct gctgcgcccc
    19141 ggaagtcact tcgaggaatc gaccatactg agcgtcgaag ccttgcaaaa tggtttgagc
    19201 aatcagtaat tccaggccac gcggcattta gcgccctcat caggagcaga gaattgccgg
    19261 atgcggcgta aacgccttat ccagcctacg ttcggcaacg gttgtaggca tgataagacg
    19321 cgcaagcgtc gcatcaggca tcgtgcaccg attgccggat gcggcgtaaa tgccttatcc
    19381 ggcctacagt cagtaacgtt tgtaggttgc ttagaactgc gattcttcag tggagccagt
    19441 cagcgcggtg actgaagacg tgccgccctg aataatagtc gtcactttat cgaagtaacc
    19501 tgtacccact tcctgctggt gagatacgaa ggtatagcca tctttcgcgg cggcaaattc
    19561 cggctgctgc actttctcaa catagtgctt cataccttcg ccctgcgcat aagcgtttgc
    19621 caggtcgaac atgttaaacc acatgctgtg gatacctgcc agggtgatga actggaactt
    19681 gtagcccata tccgacagct gctgctggaa gctggcaata gttttgtcgt cgaggttttt
    19741 ctgccagttg aacgacggcg agcagttata agccagcagt ttgcccggat atttcgcgtg
    19801 gatagcttgt gcaaagcgac gcgccagttc cagatccggc gtggaggttt cacaccagac
    19861 taggtcagca tatggcgcat acgccaggcc acggctgatc gcttgctcaa tgcccgcatg
    19921 agtacggaag aagccttcac tggtacgctc gccggtaata aattcgctgt catacgggtc
    19981 gcaatctgag gtgatcagat ccgccgcatc agcatcggta cgtgcaacca gcagggttgg
    20041 aacgcccgtc acgtcagctg ccagacgcgc cgcgaccagt ttctgaatag cttcctgagt
    20101 tggcactaaa actttgccgc ccatgtgacc gcatttcttc actgacgcca gctgatcttc
    20161 gaagtgaact gccgcagcac cggcttcaat catcgctttc atcagttcaa aggcattcag
    20221 gacaccgcca aaaccggctt ccgcatcggc aacgatcggc aggaagtaat cgacatagcg
    20281 cggatcgccc ggctcaatgc ccacggacca ttggatctga tcggcacgac ggaaggtgtt
    20341 gttgatccgc tccaccacag ctggcaccga gtttgccgga tagagcgact gatccggata
    20401 catgctggcc gccaggttag cgtccgccgc tacctgccat cccgacagat agactgcttc
    20461 aatacccgct ttcgcctgtt gcagcgcctg accgccagtc agtgcaccga ggctgttgat
    20521 gtagcctttt ttcgactcac cgtgcagcag acgccacatt ttcgctgcgc ccagttgcgc
    20581 cagcgtgcat tcaggattga ctgaaccgcg taatttcacc acatcttccg cactgtatgg
    20641 gcgagtaatg ccttcccaac gcggttgagt ccactctttc tgtaattctt caatttgttg
    20701 tgtacgggtt ttcatgtgca gatgctccat agttatgtgg tggtttacgc taacaggcgg
    20761 tagcctggca gggtcaggaa atcaattaac tcatcggaag tggtgatctg ttccatcaag
    20821 cgtgcggcat cgtcaaaacg gcccagggag aaacgttctt cgcccagttc gctggcaatg
    20881 actttcatct cttcgcccag catctggcgg aacaaggctt tggtcaccgg tttgccattg
    20941 ctcaacgttt tttgatgatg gatccactgc cagatcgagg tacgggaaat ttcagccgtc
    21001 gccgcatctt ccatcaggcc ataaatcggc acgcagccgt tgccggagat ccacgcttcg
    21061 atgtactgca cagccacgcg aatgttagcg cgcatacctt cttcggtgcg ttcaccatca
    21121 caaggtgcca gcagctgatc ggcagtaatc ggcgcgtctt gttcgcgcaa tacttcaagc
    21181 tgatttttac gggagccgag aatgtcgttg aataccgcca ttgccgtgtc cgcaaggcct
    21241 gggtgagcga tccatgtgcc atcgtgaccg ttattggctt ccagcgcttt atccgctttt
    21301 actttgttga gcacctggtt attacgctct tcatctttgc tcggaataaa cgcggccatg
    21361 ccgcccatcg caaaagcacc gcgtttatgg caggttttaa tcagcaggcg tgagtaagca
    21421 ttcaggaatg gtttatccat cgtcacggcc tgtctgtctg gcaggacacg atcgggatag
    21481 tttttcaacg ttttgatata gctgaagatg taatcccagc gaccgcagtt cagaccaaca
    21541 atatggtcgc gcagcgcgtg aaggatttcg tccatctgga acacggcggg cagcgtttca
    21601 atcagcaacg tcgccttgat ggtgccgcgc ggcagattaa agcgatcttc tgcatagctg
    21661 aagacttcgc tccaccaggc cgcttcctgc taggactggg ttttcggcag atagaaatag
    21721 ggaccgctgc cctttgccaa cagtgcctga tagttgtgga agaaatagag cgcaaaatca
    21781 aacaggctac cggggattgc ctcaccacgc caggtgacat gtttttccgg caagtgcaga
    21841 ccgcgtaccc gacaaatcaa aaccgctgga ttgggcttga gctgataaat tttgcctgct
    21901 tcattggtat agctgatggt accgttaacc gcatcgcgca ggttaatttg cccgtcgatc
    21961 actttgttcc agtctggtgc cagtgaatct tcgaaatcgg ccataaagac tttcacattg
    22021 gcgttcagtg cgttgatcac catcttgcgc tctaccgggc cagttatctc tacgcggcgg
    22081 tcttctaagt ccgcaggaat cccgcgaatt ttccaatcag catcgcgaat ggaagccgtt
    22141 tccgaaataa aatcaggcaa cgttccgtta tcaatatctt gctgctgctg aatgcgcgct
    22201 gccagaagtt tattgcgttg tggcgtaaaa tgcgtcacca gctcagtcag aaattctacc
    22261 gcttcggcag taagaatttg cttctcctgc tcgccatacg gccttgtgaa agccagttca
    22321 tcggttgttg ttgcctgttc agtcatcgtg cagctcctcg tcatggatcc gaaaacttcc
    22381 cctctgtgaa cgaaggaacg ttgtgcgctt ttcgttcagc acaactaaga ctactcattt
    22441 aaaatgcaaa atcaaaaaca atttccattt taattaaaaa tacttgataa cttattgata
    22501 acaatatgaa taaaatttat tcacttgctg aggtgcgttt cggaaataaa aaaggcaccc
    22561 gaaggtgcct gaggtaaggt gctgaatcgc ttaacgatcg actatcacag aagattaatc
    22621 cagcgttgga ttcatgtgcc gtagatcgta tggcgtgatc tgatagacgt aatagttgag
    22681 ccagttggta aacagcaaat taccgtgact acgccagctc gctcgcggtt tgttttgcgg
    22741 atcgttgtgc gggaaatagt tatacggtac atccgggtct agtccggctt ccacatcgcg
    22801 taaatattcc tgcgccagcg tttgcgcatc atattcggga tggcccgtca caaaggcaat
    22861 gcgcttatct ttgctggcaa acagatatgc atccccttct tccgtctctg ccagaatttc
    22921 cagatcggtg taatcacgaa tcaacgctgc cggaaagtca gcatagcgcg aatgcggtgc
    22981 caggaatgaa tcatcaaagc cacgcgtcag aagcgcatga ggatggagaa tatgatgctc
    23041 gtaaacgcca gagagtttgt cggtgcgagt ttgcttagga atgccgtaga ggatattgag
    23101 cgcggcctgt accgcccagc agacaaacag cgtcgaggtg acgtgatctt tcgaccactc
    23161 cagcacctgt ttgatctgcg gccagtaagc gacatcatta aactccacca ggcccagcgg
    23221 cgcaccggtt acaatcagac cgtcaaagtt ctgttcctga atatcttcaa agttacagta
    23281 gaagttgttc agatgctctg cgggcgtgtt gcgcgattca cgggaatcga tgcgcaacag
    23341 ctgaatatcg acctgcaaag gtgagtttga aagcaggcgc agaaactgat tttcagtttc
    23401 aatcttcttc ggcatcaggt taaggatcag aaccttaagt ggacgaattt cctgaccaga
    23461 cgcacgagaa gttgtcatca caaagacgtt ttcttcacgc aagaaattga cggcgggtag
    23521 ctcgtccggc acacgaatcg gcataacctg attacctcac tacatacgct tatacgttta
    23581 gacatccaga tagctgaaga taaccagaaa atttgccaat gtcgagcctg catgttgaag
    23641 gtgagaaagt ttcaaggaaa aggctacgtt agaatataag aatgacgaaa aggagagaaa
    23701 atggttatta gtattcgccg ctcacggcat gaggaagggg aggaactcgt tgcgatttgg
    23761 tgtcgatctg tcgatgccac ccacgatttt ttatcagcag agtatcgggc cgagctggaa
    23821 gagctggtgc gttcctttct gccggaagca ccgttgtggg tcgcggttaa tgaacgggag
    23881 cagccggttg ggtttatgtt gttaagtggg cagcatatgg atgcgctgtt tatcgatcct
    23941 gatgtgtgcg gctgcggcgt gggtcggatg ctggtggagc atgcgctctc gatggcaccg
    24001 gaactgacaa ccaacgttaa tgagcaaaat gagcaggcgg ttgggttcta taagaaggtg
    24061 ggttttaagg ttacgggacg ctctgaggtg gacgatttgg ggaaaccgta tccgttgctg
    24121 aatctggcgt atgtggggga ataagccgcc cctgccgtgc ctgcccaagc tggagaagac
    24181 gcgccagcgt cgcacccggc atttttttca ccaacgcaaa aaggccatcc tgacggatag
    24241 cctttcgcta aatttgatgc ctggcagttc cctactctcg catggggaga ccccacacta
    24301 ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg
    24361 ctacg
//
LOCUS       DAOFMX010000062        24146 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.062, whole genome shotgun sequence.
ACCESSION   DAOFMX010000062 DAOFMX010000000
VERSION     DAOFMX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.062"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(22..594)
                     /locus_tag="QBA20_002807"
     CDS             complement(22..594)
                     /locus_tag="QBA20_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HDQ6293272.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="QBA20_002808"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="QBA20_002808"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDQ6293273.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="QBA20_002809"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="QBA20_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDQ6293274.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="QBA20_002810"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="QBA20_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDQ6293275.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="QBA20_002811"
     CDS             complement(3556..5337)
                     /locus_tag="QBA20_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDQ6293276.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="QBA20_002812"
     CDS             complement(5330..7102)
                     /locus_tag="QBA20_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDQ6293277.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="QBA20_002813"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="QBA20_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDQ6293278.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGGWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8528)
                     /gene="cof"
                     /locus_tag="QBA20_002814"
                     /pseudo
     CDS             complement(7744..8528)
                     /gene="cof"
                     /locus_tag="QBA20_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
     gene            8627..10319
                     /locus_tag="QBA20_002815"
                     /pseudo
     CDS             8627..10319
                     /locus_tag="QBA20_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10384..11079
                     /gene="queC"
                     /locus_tag="QBA20_002816"
     CDS             10384..11079
                     /gene="queC"
                     /locus_tag="QBA20_002816"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDQ6293279.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11131..11529)
                     /gene="fadM"
                     /locus_tag="QBA20_002817"
     CDS             complement(11131..11529)
                     /gene="fadM"
                     /locus_tag="QBA20_002817"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDQ6293280.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11623..11994)
                     /locus_tag="QBA20_002818"
     CDS             complement(11623..11994)
                     /locus_tag="QBA20_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDQ6293281.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12145..14016)
                     /gene="ppiD"
                     /locus_tag="QBA20_002819"
     CDS             complement(12145..14016)
                     /gene="ppiD"
                     /locus_tag="QBA20_002819"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDQ6293282.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14208..14480)
                     /gene="hupB"
                     /locus_tag="QBA20_002820"
     CDS             complement(14208..14480)
                     /gene="hupB"
                     /locus_tag="QBA20_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDQ6293283.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14689..17043)
                     /gene="lon"
                     /locus_tag="QBA20_002821"
     CDS             complement(14689..17043)
                     /gene="lon"
                     /locus_tag="QBA20_002821"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDQ6293284.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17231..18505)
                     /gene="clpX"
                     /locus_tag="QBA20_002822"
     CDS             complement(17231..18505)
                     /gene="clpX"
                     /locus_tag="QBA20_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDQ6293285.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18631..19254)
                     /gene="clpP"
                     /locus_tag="QBA20_002823"
     CDS             complement(18631..19254)
                     /gene="clpP"
                     /locus_tag="QBA20_002823"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDQ6293286.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19501..20799)
                     /gene="tig"
                     /locus_tag="QBA20_002824"
     CDS             complement(19501..20799)
                     /gene="tig"
                     /locus_tag="QBA20_002824"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDQ6293287.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21143..21460)
                     /gene="bolA"
                     /locus_tag="QBA20_002825"
     CDS             complement(21143..21460)
                     /gene="bolA"
                     /locus_tag="QBA20_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDQ6293288.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21765..22343
                     /locus_tag="QBA20_002826"
     CDS             21765..22343
                     /locus_tag="QBA20_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ6293289.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22387..23862
                     /gene="ampG"
                     /locus_tag="QBA20_002827"
     CDS             22387..23862
                     /gene="ampG"
                     /locus_tag="QBA20_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDQ6293290.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5656 a         6655 c         5818 g         6017 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagcccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcagacgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cgctaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgtggcaa aagtgagggt aatgtcgcgt tcacgcagtc gcgccaaagt agagagggtt
     8461 ttctcaccta aatgatggtc aggcatcaat aaagtgccat ccatatcaaa tgctaccaga
     8521 cgagccattt ctttctccac tctgtgacac ggttaatggt gattgagtat cacctgatat
     8581 atacggaagt aatagtgaat agttaataat attgttccgg gtttatatgc gattgctcaa
     8641 ccgtcttaac cagtatcaac gtctgtggca accttcagcc gcaaaccgtc accgtcagcg
     8701 aactggccga acgctgtttt tgcagcgaac gccatgttcg tacgctgttg cgtcaggcac
     8761 aggaggcggg atggctggag tggcaggcgc agtcaggacg cggaaagcgc ggacaattac
     8821 gctttctggt cacgccggaa tcgctacgca atgcgatgat ggaacaggca ctggaaaccg
     8881 gaaagcagca agatgtgctg gagctggcgc aactggcccc aggtgagctg cgcactctgt
     8941 tacagccatt tatgggcgga caatggcaaa acgatacacc cacgttgcgt attccctact
     9001 atcgcccgct cgaaccgcta caaccaggct ttttgcccgg ccgtgccgag cagcatctcg
     9061 ccgggcagat attttccggc ctgacccgct tcgataataa tactcagcgc ccgattggcg
     9121 atttagcgca tcactgggaa acctctactg acaggttacg ctgggacttt tatcttcgtt
     9181 caaccctaca ctggcataac ggcgatgcag taaaagcctc acacttacac cagcgattat
     9241 tgatgctgtt acaactgcca gcactggatc aattatttat tagcgtgaag cgtattgaag
     9301 tcacccatcc gcagtgtctg accttctttt tacatcgccc cgattactgg cttgcgcacc
     9361 ggctggcgag ctattgcagc catctggcgc atccgcaatt cccactgatc ggcactggtc
     9421 cttttcgctt aacacaattc acagcggagc tggtgcgcct ggagagccat gattattacc
     9481 atttgcgtca tccgctgctt aaagcggttg agtactggat aactccgccg cttttcgaaa
     9541 aagatttggg aaccagttgt cggcatcccg tgcaaatcac catcggcaaa ccggaggagc
     9601 tgcaacgggt cagccaagtc agtagtggca tcagtttagg tttttgctat ttaacgttgc
     9661 gcaaaagtcc ccgactctcc ctctggcagg cgcgaaaagt gatctccatt attcatcaat
     9721 ccggtttatt acaaacgtta gaagtcggag aaaacctgat caccgccagt catgcattac
     9781 tgccaggctg gactattccg cactggcaag taccggatga agtcaaacta ccgaaaacct
     9841 tgacgctggt ttatcaccta ccgatagaac ttcataccat ggcagaacgc ctacaggcga
     9901 cactggcagc ggaaggctgt gaactcacaa ttatttttca taacgcaaaa aactgggacg
     9961 acacgaccct actggcacac gcagacctca tgatgggcga cagattaatt ggcgaagcac
    10021 cggaatatac tctggagcaa tggctacgtt gcgatccact atggccacat gttttcgacg
    10081 ctccagcata tgcacatctg caatcgacgc tggacgcggt gcaagtaatg cctgatgagg
    10141 aaaaccgatt taatgccctg aaagcggttt ttagccagtt aatggcagac gcgacgctga
    10201 cgccgctgtt caactatcac tatcgcatta gtgcccctcc cggcgtgaac ggtgtgcgac
    10261 tgacaccgcg cggctggttt gaatttaccg aagcctggct tcccgcgccg tcgcaatgaa
    10321 tagctggtcc gggttaatcg caggcgttac cataacgctt ttattgttca agcaggatta
    10381 tctatgaaac gtgctgtcgt tgtgttcagt ggaggccagg attccaccac ctgtctggtg
    10441 caggcattac aacaatatga tgaagtccat tgcgtgacgt tcgattacgg tcagcgacat
    10501 cgcgcagaaa tcgacgtggc acgcgaactg gcgctgaaac tgggagcacg cgcgcataag
    10561 gttcttgatg taacgctgct caacgagctg gcggtcagca gcctgacacg tgacagcatt
    10621 ccggtgcctg attatgaacc tgaagccgat ggcatcccga atacgtttgt cccagggcgt
    10681 aatattttgt tcctgacgct ggcggcaata tatgcgtatc aggtaaaagc agaagccgta
    10741 attaccggcg tctgtgaaac ggatttctcc ggctacccgg attgccgcga tgagtttgtg
    10801 aaagcactaa accatgccgt cagtttgggc atggcgaaag atattcgttt tgaaacgccg
    10861 ctgatgtgga ttgataaagc ggaaacctgg gcgctggcag attattacgg caaactggat
    10921 ttagtccgta acgaaacgtt gacctgctat aacggcatta aaggcgacgg ttgcggtcat
    10981 tgtgcggcat gtaatttacg cgccaacggt ttgaatcatt atctggccga taaaccgacg
    11041 gtgatggcag cgatgaagca gaaaaccggg ttgaggtaat tattccgggt gtcgccggat
    11101 gcggcttgag catccggcac cacaaaacgt ttacttaacc atctgctcca gcttttcgcg
    11161 caattcccct tccagagcta atgctttctg cgttttaaga tcaatacaaa caaacgtaat
    11221 aagcgcatcc gctaccacct gcccttccgg ctccagtgta atgacctggt ttaagatgcc
    11281 gcttttaccg tttaattgct gcaactgact ggtaatagtt aacaggtcgc ttaataccgc
    11341 tgggcgacga tagttaatat tgatattgac cacgacgaag gcgatgttat gggccgtcat
    11401 ccactgaaaa ctgtcgctat tttccacccc atcccagcgg gcttcttcga gaaattcaag
    11461 gtagcgggcg ttgttgacgt gctggtaaac gtcgagatga tatccacgaa ctttgatttg
    11521 tgtttgcata gcgcaataac cttacgttgt tgttataagt acagaggtca taactggtat
    11581 gacctcttca gtctggcaaa aaattgccac tatgcaaatt aattacaggg ttaataccgc
    11641 cagattacgt tccaccagcg aattgcccat ccccggcacc tgctttagat cctccaccgt
    11701 tttaaacgga ccgtactctt cgcgataact gacaatcgcc tgcgctttct tcaggccaac
    11761 gccattcatc gcgcgggcta gctcttccgc gctggcatta ttaatgctga cccgggtgcc
    11821 ttcttcgtca ctggctttcg ccggtactgc tgctttactt tgtgctgcag gagcttccgc
    11881 tttggtttct accgccgtcg gtttcgccac agaagctgcc gccagcgcgc tatgagacat
    11941 tccggcacag gccagggaca gggtaatgag cagtgcttta attccgtgtt tcatactgtt
    12001 ttctccttgt ttgttaacag tggaatcacc ttaacgggag tgaaaacagt aaacaaatgg
    12061 caatgttcag aaatggaaaa ggccgcgaaa gcgacctttt cttgttacag ctgcaacgca
    12121 tttttttgcg aggcggcttc aggattattg ctgttccagc gcatcgccaa ttttgatttt
    12181 cgcctcttta cgcaggttac tcatcagagc ttcaaagacg atttgtgcgt tgttctgggt
    12241 gataccctgc accatcgctt ttttctgatc ttccggcatt gaaccttgtt tcacttcatc
    12301 cagcgccagc agaaccacat taccttgcat atcggtcgcc ataccgtagc tcggtttgtc
    12361 tttcgctggc agtggcagtg caaacgccgc ctggctaatc gggtcacgac cggaacggct
    12421 taaggttttc ggctcgccaa atttcagacc ggcagcctgc atagcttccg cacctttgcc
    12481 ggctttcaaa tcaaccagca gtttctcagc atccactttc gcctgttgtt cagctttgtt
    12541 gtgctgaacc agtgccttaa cttgttcctg aacatctgcc aacggtttca ccgcttccgg
    12601 tttgtgctcg ctgacgcgca gcacgaatgc gcggtcgccg tctacggtga tgatgtcaga
    12661 gttgatgccc gacgcgccgt tttcacctac cagaccgccg ttaaagatag cgtcggcaac
    12721 cggcttgaag ttcaactctt ccggcaggtt atctttgctg aaccaacccg tctgagtggc
    12781 tttaacgccg gcagcttgct ctgcaccggc cagagactcg gtgtcgttgc ttgccgcatc
    12841 gctcactttc tgctgcagcg catagtacgc atcgagggct ttttcgtgtt tcactttcgc
    12901 cgcaatgtcg tcacgtactt cgtctaacga tttcactttc gctggctgaa tgtcgtccag
    12961 acgtacaatc aggaaaccga ccgaagattt gatgacacca gacagttggc ctttttcttt
    13021 cagaccagca tttttcagtt cgtcggggat agtggcatct tctaaccaac ccatatcgcc
    13081 gccgttacgg gcagagataa tatcggcaga tttttctttg gctaatgcag caaaatcacc
    13141 gcctttattc agctcatcaa gtaccgcttt cgcttcatct tcagttttgg tctggatgat
    13201 gctgtagcgg gtacgctgcg gctgggtgaa ttgatcctga tgctgatcgt agtagctctg
    13261 gatatccgca tcgctaaccg gttgctgcat cgttgcggca accagcttga tgtaactcac
    13321 gcggaattgt tccggcgtca tgaaattgtt tttgttttgt tcgtagtagc tggcaatctc
    13381 ctgttcggtc acaggctgct tcgccgccag cgcgttaaca tcgatagtcg cctcacgcac
    13441 cacgcgttgt tgcgcgacca gtgccgccag ctcgtcggtt tcacctttca gcataaaatc
    13501 ggtaccggca acgccgttaa tcagctgttg ggtagtgagc tggttacgca gcgcctgggc
    13561 gtactgatcg gcggtcatcc ccatctggtt gaggataccg ttatagcggc tgttatcaaa
    13621 tttgccgtca acctggaagg ctggggtcgc gaaaatcgcc tgtttaacct gctcatcact
    13681 gatacccagt ttcagctcac gagcgtactg atccaacagc gcctcgtcga tcagacgatt
    13741 cagcacctgt tgacgcaggg ttttcatata gccttcgttc gctgccagct cggagtattg
    13801 atcgcccagc tgttgctgca tacgattacg ctcgctgtta aaggcgttct cgaactgccc
    13861 acggctgatt tcctggtcat tcacttttgc ggcgtagtta ttgcctccgc caatcaggta
    13921 accactcacg ccggtcaata tgaacgacac gataatgata ccgaaaataa tcttgagcac
    13981 gagactgttt gcagccgtgc gtaagctgtc catcatggtg taacaacact ccgctgtaga
    14041 tgactgtgta cctcacgcaa cgtatcgatg cgcgcaaagg cctattgtga caagaaacgg
    14101 gggcaattgt cagcccacaa gacacagaaa gtcaccgaat acaaataaaa aaggcacatc
    14161 agtagatgcg cccttgaact tcgtcacatc cccactgggg acaacgctta gtttaccgcg
    14221 tctttcagtg ctttacctgc acggaagctc ggtactttag cggcagcgat ggtgatctct
    14281 ttaccggtct gcgggttgcg gccagtacgg gcagcacgct ctttaacggc aaaagtacca
    14341 aaacctacca gtgctacatc atccccttct ttcagagatt cagttacgga agcaataata
    14401 gcatctaacg cacggccagc cgcagcttta gagatatcag cccctgcagc aatcttgtcg
    14461 atcaattgag atttattcac tcttctcttc ctctttataa tttatatcgc acttgaatcc
    14521 ttcaaggtac gaacgcgcag cagttatatc aggccagcca tccccttaca acacccctta
    14581 ataagggcaa gcccgatccg ctatctaact tagcgagaca aaaaaaggct ggcaagcccg
    14641 aattagcctg ccagccctgt ttttattagt gcattttgcg cgaggtcact attttgcagt
    14701 cacaacctgc atgccagacg gttcattttg cagcgccaga gtcagaactt cctcaatgcg
    14761 cttcacagga tgaatgtcca gatcggcaat tacgttgtca ggaatctctt ccagatcgcg
    14821 tttattttcg aacggaatta gcactgtttt aatcccgccg cgatgcgctg ccaggagttt
    14881 ttctttcaaa ccaccgatcg gcagtacctg accacgcaga gtgatctcac cggtcattgc
    14941 cacatcggca cgaaccgggt taccggtcag gcaagaaacc agcgcggtgc acatagcaat
    15001 accggcactc ggaccatctt tcggcgtcgc accttccggt acgtggacgt ggatgtcacg
    15061 tttttcgtaa aaatcagggt tgatccccag tttttccgca cgcgcacgaa ccaccgttaa
    15121 cgccgcctga atagactcct gcatcacttc gccgagcgaa ccggtatagg tcagtttgcc
    15181 tttacccgga acacatgcgg tttcaatggt cagcaagtca ccgcccactt ccgtccacgc
    15241 cagaccgatt acctgaccga cacggttttc gttatcagcg cgaccatagt cgaaacgctg
    15301 aacaccgagg tagtcatgca ggttatcgcc gttaatttcg atatgtttta atgacttatc
    15361 gagcagtaac tgcttaaccg ctttgcggca cagtttggag atttcacgct ccagaccacg
    15421 cacgcccgcc tcacgggtgt agtaacgaat aatgccgata atggcgctat cgtcgacggt
    15481 cagctcacct tttttcagtg cattacgttc aatctgcttc ggcagcaggt gacgtttggc
    15541 gatgttcagt ttttcatctt cggtataacc ggagaggcga atcacttcca tacgatccag
    15601 cagcggtgcc ggaatgttca tggagttcga cgtcgcgaca aacatcacgt cgctgagatc
    15661 gtaatccact tccaggtagt ggtcgctgaa cgctacgttc tgctctggat ccagcacttc
    15721 aagcagtgca gaggccggat cgcctcgcat gtcagaagac attttgtcga tctcatcgag
    15781 caggaacagc ggatttttca cgcccacttt cgccattttc tggatcagtt tacccggcat
    15841 agaaccgatg taagtacggc ggtgaccacg gatttccgct tcatcacgca cgccgcccag
    15901 cgccatacgg acatatttac gcccggtggc tttggcaatg gactgaccaa gagaggtttt
    15961 acctaccccc ggcggcccta ccaggcagag gatcggtccc ttgattttgt tgacacggct
    16021 ttgaaccgca aggtactcaa ggatgcgatc tttcacgcgc tccagaccat aatggtcggt
    16081 atcaaggatt tcctgcgcct gacgcaggtc ttttttgacc ttgctgcgcg cattccacgg
    16141 tacctgtacc atccagtcga tataaccacg cactacggtc gcttctgccg acatcggaga
    16201 catcattttc agcttctgca actctgcttc cgctttctct tttgcctctt tcggcatctt
    16261 cgccgcgtcg attttgcgct tcagggcttc gttttcgtcc ggcacgtcgt ccatttcacc
    16321 gagttctttc tgaatagctt tcatttgctc gttcagatag tactcacgct gggatttctc
    16381 catctgcttt ttaacgcggt tgcgaatgcg tttctcaacc tgcagcagat cgatttccga
    16441 ttccatcatt gccatcagat attccagacg ttcgttaacg tcggacatct ccagaacgga
    16501 ctgtttgtca gccagtttca gcggcatatg tgcagcaatg gtatccgcca gacgcgccgg
    16561 atcgtcgatg ctattcagcg acgtcagcac ttctggtggg atttttttgt tcagcttgat
    16621 gtagccttcg aactggctga ttgcagtacg caccagcact tcctgttccc gctcatcaat
    16681 ggtcggcgac tccagatact ccgccttcgc agaaaagtgt tcgccattgt cagagagcgc
    16741 agaaatacgc gcgcgctgta acccctcgac cagcactttg acggtgccgt caggcagttt
    16801 cagcatctgc aatatagagg ccacggtccc gacggtgaaa agatcgttta cacccggctc
    16861 atccgttgaa gcttctttct gcgcgaccag cataattttt ttatcatggt ccatcgccgc
    16921 ttccagacaa cggatagatt tttcccgccc gacaaataag gggatgacca tgtgcggata
    16981 aaccaccaca tcgcgcagcg gcaatacggg gatttcaatg cgttcagaac gctcaggatt
    17041 catagagctc tctcttagtt taatttccgc caggtaatca gatgacacga ctgtgcttca
    17101 cgccatctat taacatgtac gtcagtatat ggggatgttt cccccacatt caacgccgag
    17161 aatagaggaa aaattaaagg ggagataaaa tccccccttt ttggttaact aattgtatga
    17221 gaatggttaa ttattcacca gatgcctgtt gcgcttccgg cttaccataa atcagcaacg
    17281 gtttgctttg accatcaatt accgactcgt cgataaccac tttttcgacg tcttccatgg
    17341 acggcagatc gtacatggta tcgagcagtg cggcttctac gatggaacgc aggccacggg
    17401 caccggtttt acgcgccatc gctttcttag cgatagcatc cagcgcctcg tcacggaatt
    17461 ccagatccac gccttccaga ttaaacagcg cctgatactg cttggtcagg gcgtttttcg
    17521 gctctttgag gatctgaatc agagcttctt cgctcagttc attcaacgtt gctacaaccg
    17581 gcagacgacc aatgaactca gggataagac caaacttgat cagatcttcc ggttcaacct
    17641 gcgccagcag ctcgccttcg cttgctttgt cggacttcgc ttttaccgtc gcgccaaaac
    17701 caatgccgga gccggtttct acacggtggg aaatcacttt atccagaccg gcaaacgcac
    17761 cgccacaaat aaacaggatc ttagaggtat caacctgcaa gaattcctgc tgcggatgtt
    17821 tacgcccacc ttgcggtgga acagcagcta ccgtaccttc gatcagtttc aacagtgcct
    17881 gctgtactcc ttcaccggaa acgtctcggg taatggacgg gttgtctgac ttacgagaaa
    17941 tcttgtcgat ttcatcgatg tagacaatcc cgcgctgcgc tttctgtacg tcgtaatcgc
    18001 acttttgcaa cagcttctga atgatgtttt caacgtcttc gcccacataa ccggcttcgg
    18061 tcagcgtggt tgcgtcggcc atggtgaacg ggacgtccag caggcgcgcc agcgtttcag
    18121 ccagcagcgt tttaccggaa ccggtcggac cgatcagcag aatgttactt ttgcccaact
    18181 cgacaccatt gctggtatcg ccgttgcgca gacgtttgta atggttgtat accgcgaccg
    18241 ccagcacttt tttcgcctgt tcctggccga taacgtaatc gtccaggtgg ttgcgaattt
    18301 catgcggcgt cggtagcgca ctgcgttcac gatgcggtgc aacttcttta atctcttcgc
    18361 gaatgatgtc gttacataaa tcaacacatt cgtcgcagat atacacggat ggaccggcaa
    18421 tcagcttgcg cacttcatgc tggcttttgc cgcaaaaaga gcaatacagc aatttgcctg
    18481 agccatcttt gcgtttatct gtcatgggtc aaaacctctt ctttgttctt tgtgccgcac
    18541 acgacgacgc aaatgccatt ctcaggcgca agccgcttac agcgttgtgc cgccctagat
    18601 aagtatagcg gcacagttgc gcctctggca tcaattacga tgggtcagaa tcgaatcgac
    18661 cagaccgtat tccaccgctt caggggcgga aaggaagcga tcgcgctcgg tatcacgttc
    18721 aatctgttct aatgattgac ccgtatgaag cgccataagt tcattcatgc gccctttaac
    18781 tttcagaatt tcacgggcat gaatttcgat atcggtcgcc tggccctggt agccgcccaa
    18841 cggttggtga atcatcacgc gcgaattcgg caggcaaaaa cgtttacctt ttgcccctgc
    18901 ggtcaacaag aaagcgccca tcgaggccgc ctggcccata cagatggtgc taacatcagg
    18961 cttgataaac tgcatggtgt catagataga catcccggca gtgatcaccc cgcctgggga
    19021 gttaatgtac agatagatat ctttttctgg gttttccgct tccaggaaca gcatctgcgc
    19081 cacaatcagg ttagccatgt ggtcttcaac ctggccagtc agaaaaatga cgcgttcctt
    19141 aagtagacga gaatagatat caaaagagcg ctcaccgcgt gaggtctgtt caatgaccat
    19201 cggcaccagc gccatatggg gtgcaaagtt atctcgttcg ccgctgtatg acatttccgt
    19261 ctcctggata aaattgaaaa aaacctgctg taccgattgt aaccttatgg acggattatc
    19321 agctagtccc tgtgttatgg gtacggcatc accatatttc aagcataaca atcttttgtt
    19381 gttacgctaa caccgaaaga ggcttttcgc acggctccat gcaaaattca tgacaaaaaa
    19441 aagcccacca cctggcggtg acgggccttt gtgcgaattt agcgcgttat gctgcgtaaa
    19501 ttacgcctgc tggttcatca gctcgttgaa agtggtttct ttttcagtca ctttcgcttt
    19561 cgccagtaca gcttcaacag cctgttcttc cagagcaaca ttgcgcatgt tgtccatcag
    19621 ttctttgttt ttgctgtaga actcgataac ttctttcgga tcttcgtacg cagaagccat
    19681 ctcttcgatc aggcctttca cgcgctcttc gtcagctttc agctcgttgg tgcggataac
    19741 ttcgcccagc agcaggccaa caactacgcg gcgtttagcc tgttcttcga acagttcgcg
    19801 cggcagttcc agagcttgtt tttcgttgcc accgaaacgc tgtgcagcct ggcgacgcag
    19861 aacgtcgatt tcgctgtcga tcagcgcagc cggtacgtcg atgtcgttag cttttaccag
    19921 accttcgatc gcctgagact taacgcggtt acggatggcg ctcttcagct cgcgctccat
    19981 gtttttacgc acttcagcgc gcagaccttc tacggaacca tcttcaacgc cgaaacgttt
    20041 gatgaattct gcagtcagtt ccggcagttc acgctcttca actttcttca ggttgatagc
    20101 gaatttcgct gctttacctt tcaggttttc tgcgtggtat tcttccggga aggtcacgtc
    20161 gatggtgaac tcttcgccag ctttgtggcc tttgataccg tcttcaaagc ccgggatcat
    20221 acgaccctgg cccatcgcca gtacgaaatc agacgcttta ccgccttcga actcttcgcc
    20281 gtctacagaa ccggtgaagt cgatggttac gcggtcttct gcttcaacag cgccgtcttt
    20341 ttctttccag gtcgcctgct gtttacgcag agtatccagc atgccgtcaa cgtcagcgtc
    20401 ggtcacttca acgatcggtt tttcaacttc gatcgcttcc agaccctgca gttcaacttc
    20461 cggataaact tcaaactcta cagagtaagt gaagtcttca cccagcttgt attcgcccgg
    20521 aacataagtc ggtgcgccag ccggattgat tttttcttta atgatggcgt caatgaagtt
    20581 acggctcatc aggtcaccca gaacgtcctg gcgtacagac gcgccataac gctgagcaac
    20641 gatattcatt ggcactttgc ctttgcggaa gccgtcaata cgtacttttt tcgcaacgtt
    20701 gaccagctcg cttttaacag cggtctcgat gctgtcagca gcgatagtaa tcgttacacg
    20761 gcggccaagg ccttgagtgg tttcaactga aacttgcatc ttgttacctc aaaaaatcac
    20821 agtgctcggt caactcttac cccgcaagca caggttgttg gcttcgcgga accgggatgt
    20881 tcttataatc aatcacattc cctgttacca gaatcatccc gaaagcattc aaataggacg
    20941 cggcattata gcggcatcac ttttatgagt cgagaacggt tattgcgcgc tgtcgcgtat
    21001 tttttcacaa ttcccgggca attttcgtct gaatgctggg caaaaagcgc aaaatccaga
    21061 caaaacaaaa cggcccgcag gccgtttttc ataaaatctt tagcaacata ctggaaaagc
    21121 gccgacagtt gcaaatgcgt ttttacgcga tgcttcctgc tccacgacag ggaggagagg
    21181 caaagacggt gtcctgcaac ccttcccact ccttaatagt gtaagtatgc agagccagcg
    21241 catgaacggt agtagagagt tcctccgcta aagtactgta aatcattcga tgacgattca
    21301 gaaaacgttc acccgtaaaa cgatcgctga ccagcacaac tttaaaatgg ctttcagagc
    21361 cggctgggac attgtgacga tagctttcat ccactacttc gaggaatacg ggttggaacg
    21421 ccgcccttaa tttttcttct atccgctcac gtatcatcat gaaatatctc ctccgacaac
    21481 gctgagatgt catccatccc tttaaatact agccgctttt accgtttcca ttgcagctta
    21541 acaacaaata tttaggtttc gtcctggttt ttccgtcaag cgtatgaagt ttactgattt
    21601 aaagattgaa acacaggcct cgcatcagcg tttcttttac caggggtgat tatttcgtca
    21661 acatgatgaa tttacatgcc ttacccactt cccctcgccg ttggcaatgt tatgatggcg
    21721 ggaattttta ccaaacacgc ttaagagtct ctgagagccc gaacatgttc aaaaaaatcc
    21781 tcttcccgtt agttgctctg tttatgcttg caggatgcgc aaaaccgcca acaactattg
    21841 aagtttcccc gacgattaca ctgccacagc aggatccaag cctgatgggc gtcaccgtaa
    21901 gcattaatgg tgccgatcag cgtaccgatc aggcgctggc aaaagtcacc cgcgataatc
    21961 aaatcgttac cctgaccgcc tcccgcgctc tgcgtttcct gctgcaagaa gtgctggaaa
    22021 aacagatgac cgcgcgtggt tacatggttg gtccgaatgg cccggttaat ctgcaaatca
    22081 tcgttagcca actgtatgct gacgtgtccc agggcaacgt gcgctacaac atcgctacca
    22141 aagcggatat cgccatcatc gctaccgcgc agaatggcaa caaaatgacc aaaaactatc
    22201 gtgccagcta caacgttgaa ggtgcgttcc aggcctccaa caaaaatatc gccgatgcgg
    22261 ttaacagtgt gctgaccgat accatcgctg atatgtctca ggacaccagc attcacgaat
    22321 tcatcaagca gaacgcgcgt taatttctgc cctctggccc ggtgcaagcc gggcctgtag
    22381 acgcccatgt ccagtcaata tttacgtatt tttcaacagc cgcgttcagc catattgctg
    22441 atcctgggtt ttgcttccgg gctaccgctc gccctgacat ccggcacctt acaggcctgg
    22501 atgacggtcg aaaatatcga tctcaaaacc attggtttct tctctctggt aggccaggct
    22561 tacgttttta aattcctctg gtcaccgctg atggaccgct acacgccccc attttttggg
    22621 cggcggcgcg gttggctgct cgccacgcaa atcctgttat tagtcgccat tgcggcgatg
    22681 ggttttctcg aaccaggcac ccaactccgc tggatggcgg cgctggcggt ggtgatcgct
    22741 ttttgctctg cctcccagga tattgtcttc gatgcgtgga aaaccgatgt gctcccggca
    22801 gaagaacgtg gtgcgggcgc ggcaatcagc gtgctgggtt accgtttagg gatgctggtt
    22861 tccggcggcc tagccctgtg gctggcagat aaatggttgg gctggcaggg catgtactgg
    22921 ttgatggcgg cgctgttgat cccctgtatt atcgcgacgt tgcttgcacc agaaccaacc
    22981 gacaccattc ctgtgcccaa aacgctggaa caagcggttg ttgcacctct gcgagatttc
    23041 tttggtcgca ataatgcctg gcttattttg cttcttatcg tgctgtataa gctgggcgac
    23101 gcattcgcca tgagcctgac aaccacgttt ttgattcgcg gcgtcgggtt tgatgcgggt
    23161 gaagtaggcg tggtaaacaa aacgcttggc ttactggcga ccattgttgg cgcattgtac
    23221 ggtgggattt tgatgcagcg cctgtcgctg ttccgggcgc tgctgatttt cggcatttta
    23281 caaggtgcgt ctaacgctgg ttactggctg ctgtcgatta ctgataagca tctctacagc
    23341 atgggcgcag ccgtcttttt cgaaaacctc tgtggcggga tgggcacatc agcctttgtc
    23401 gcgctgttaa tgacgctgtg taataagtca ttttccgcta ctcaatttgc cctgctctca
    23461 gcgctttccg ctgtagggcg agtttatgtc ggccccgtgg cgggttggtt tgttgaagca
    23521 cacggctggt cgacattcta tctattctcc gtcgccgctg ccgtaccggg gcttattttg
    23581 ctgctggttt gccgccagac gcttgaatat acacgagtaa atgacaactt tatctcccgt
    23641 accgaatatc cggcaggtta tgcctttgcc atgtggacac tggcggcggg cgtcagcctg
    23701 ttggccgtgt ggttacttct gttgacgatg gacgcgctgg atttgacgca cttctctttc
    23761 ctgcctgctc tgctggaagt cggggtttta gtcgcccttt ctggcgtcgt gcttggtggt
    23821 ttgctggatt atctggcgct acgaaaaacg catctgacgt aatctgtaaa tattatttag
    23881 ctgtagtaat cactcgccgt aattattacg gctaaataaa catcagcatc gcttattaat
    23941 atttgttctt ttgtgcggct tagcgtttgg aaattattga cgccatttat aaattctatt
    24001 tttcttacac gattcagcta atgagtcttt attttctcac cacccagttg tcactttaat
    24061 gataattatt tgttaaataa ttgttttatt tcgcattggt tataccaatt gcccgccaag
    24121 actccttctg cactcccctt ttgtta
//
LOCUS       DAOFMX010000063        22829 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.063, whole genome shotgun sequence.
ACCESSION   DAOFMX010000063 DAOFMX010000000
VERSION     DAOFMX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.063"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="QBA20_002828"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="QBA20_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDQ6293291.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="QBA20_002829"
     CDS             1207..1869
                     /locus_tag="QBA20_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ6293292.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="QBA20_002830"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="QBA20_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDQ6293293.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="QBA20_002831"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="QBA20_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="QBA20_002832"
     CDS             2795..3100
                     /locus_tag="QBA20_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDQ6293294.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="QBA20_002833"
     CDS             3103..3507
                     /locus_tag="QBA20_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDQ6293295.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="QBA20_002834"
     CDS             3497..3796
                     /locus_tag="QBA20_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDQ6293296.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="QBA20_002835"
     CDS             3982..4278
                     /locus_tag="QBA20_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDQ6293297.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="QBA20_002836"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="QBA20_002836"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDQ6293298.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="QBA20_002837"
     CDS             5627..5995
                     /locus_tag="QBA20_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDQ6293299.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="QBA20_002838"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="QBA20_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDQ6293300.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="QBA20_002839"
     CDS             7203..7904
                     /locus_tag="QBA20_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDQ6293301.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /locus_tag="QBA20_002840"
     CDS             7927..8091
                     /locus_tag="QBA20_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293302.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /locus_tag="QBA20_002841"
     CDS             complement(8303..9613)
                     /locus_tag="QBA20_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDQ6293303.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..10972)
                     /locus_tag="QBA20_002842"
     CDS             complement(9641..10972)
                     /locus_tag="QBA20_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDQ6293304.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="QBA20_002843"
     CDS             complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="QBA20_002843"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDQ6293305.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12683..13072)
                     /locus_tag="QBA20_002844"
     CDS             complement(12683..13072)
                     /locus_tag="QBA20_002844"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDQ6293306.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13086..15275)
                     /gene="tdcE"
                     /locus_tag="QBA20_002845"
     CDS             complement(13086..15275)
                     /gene="tdcE"
                     /locus_tag="QBA20_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDQ6293307.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIACCVSP
                     MVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLD
                     HFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVATDSLSA
                     IKYARVKPIRDENGLAVDFEIDGEYPQYGNNDERVDSIACDLVERFMKKIKALPTYRN
                     AVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKL
                     PFTYAKDGISYTFSIVPAALGKEDPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNR
                     EMLLDAIEHPEKYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15309..16517)
                     /gene="tdcD"
                     /locus_tag="QBA20_002846"
     CDS             complement(15309..16517)
                     /gene="tdcD"
                     /locus_tag="QBA20_002846"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDQ6293308.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16543..17874)
                     /gene="tdcC"
                     /locus_tag="QBA20_002847"
     CDS             complement(16543..17874)
                     /gene="tdcC"
                     /locus_tag="QBA20_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDQ6293309.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(17896..18885)
                     /gene="tdcB"
                     /locus_tag="QBA20_002848"
     CDS             complement(17896..18885)
                     /gene="tdcB"
                     /locus_tag="QBA20_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDQ6293310.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(18984..19922)
                     /gene="tdcA"
                     /locus_tag="QBA20_002849"
     CDS             complement(18984..19922)
                     /gene="tdcA"
                     /locus_tag="QBA20_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDQ6293311.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20112..20456
                     /gene="tdcR"
                     /locus_tag="QBA20_002850"
     CDS             20112..20456
                     /gene="tdcR"
                     /locus_tag="QBA20_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDQ6293312.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20712..21251
                     /locus_tag="QBA20_002851"
     CDS             20712..21251
                     /locus_tag="QBA20_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293313.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            21273..21620
                     /locus_tag="QBA20_002852"
     CDS             21273..21620
                     /locus_tag="QBA20_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293314.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21789..22439
                     /locus_tag="QBA20_002853"
     CDS             21789..22439
                     /locus_tag="QBA20_002853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293315.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6155 a         5761 c         5258 g         5655 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtagc gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ttgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 caattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    10621 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    10681 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    10741 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    10801 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    10861 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
    10921 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
    10981 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    11041 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
    11101 cttcacaatc aagactttaa aataacggag atggataaga attttctact aaattaatcg
    11161 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
    11221 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    11281 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    11341 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    11401 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    11461 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    11521 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg
    11581 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    11641 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    11701 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    11761 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    11821 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    11881 cgcagcgcta cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    11941 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    12001 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    12061 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    12121 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    12181 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    12241 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    12301 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    12361 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    12421 acatccgtgg catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata
    12481 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    12541 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    12601 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    12661 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    12721 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    12781 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    12841 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    12901 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    12961 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    13021 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    13081 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    13141 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    13201 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
    13261 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccaac aaggttggtt
    13321 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    13381 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    13441 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    13501 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    13561 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
    13621 tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    13681 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    13741 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    13801 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    13861 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    13921 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    13981 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    14041 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    14101 gggctgacgc agcaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc
    14161 ggtgccggcc ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc
    14221 gtacgaccgt ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag
    14281 aacagcgaat caaattccgg tgtacgcagg aagcgtacca tacggatctt catgatgaag
    14341 tgatcgatca gttcctgtgc ctgctgctca ttgaggacgc cagctttaaa gtcgcgctca
    14401 atgtagatat ccaggaacga tgccgtgcgc cccagcgaca tcgcgccgcc attttgcgat
    14461 ttcactgccg ccagataagc gaagtagagc cactgcaccg cttcctgtgc attctgcgcc
    14521 gggcgagaga tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga
    14581 cgatgctctg ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc
    14641 agacgagact ggagatcggc aaattgcagt tcgcgttcac gcaccagata actgatgcca
    14701 tacagcgcta cgcggcgata gtcaccgata atgcgcccac gaccatagcc atctggtaaa
    14761 ccggtcagca cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg
    14821 ccctggttat gggttttacg cagatcggta aacagatatt caaattcact gtccatttca
    14881 cggccatagg cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtagcgca
    14941 cgtttcaacg gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat
    15001 cccgcatcat gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg
    15061 gttgcgtttt cgatacggat gccttccatt accttttccc acaattccgt ggtggcaggc
    15121 gtcgcttcgg cgagaaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa
    15181 tcgcggacat taatttcgct tttccagtcc gtacctttaa agccaagcca tgcgtcagcg
    15241 tacagcttat cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt
    15301 caactaaatt atgcaaattc tgcgggcgcg ttaactttac ctaaatgaat ggcatccaaa
    15361 gcaatcattt tttcttcgtt agtcggaata acgacgcaaa tgacatgcgc attttcactg
    15421 gaaacaattc gctcgccaaa agagttagat cgattattca tttctgtatc aatctctacg
    15481 cctaatacag ccaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt
    15541 ccgccggtga atataattcc atccagccga cgtaatgaag ctgcatgtcc ggcaatatga
    15601 cgggcaattc ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc
    15661 caggcttttt ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac
    15721 tctttattca ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag
    15781 gacatcgcac caaagtcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc
    15841 gtcattccca ttgaggtgtc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca
    15901 ttgccaagat gcgccacaac caggccggag tcatcttccg ccagattcag cagcgaatgt
    15961 gcgcgctggg aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac
    16021 tcttcataat atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg
    16081 aaactggtat caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca
    16141 ataccactta aatttgcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta
    16201 tcaatgactt catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg
    16261 cggtggccaa ttaaggccac gctgtcattt aaattccgtt tttccagttc aaatgcaatt
    16321 gccttcaatg caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg
    16381 gataagaatg cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacaa
    16441 tcgctggcat cgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca
    16501 accggaaatt cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac
    16561 gatgttcagg atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg
    16621 gtatttcgcc agagacggcg ctttacggat agcatacatc ggcaacaggc acagcaggga
    16681 tgcgataatc ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc
    16741 aacaacccag gtggagccca tgatgaagat catgctgaga gtattcagtt tacccagcga
    16801 cactttggtt ttgtcacctt tataaccgaa cttcagaatc agaccattca agccttccag
    16861 cgtccccaga tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc
    16921 cgcatattcc agtgtaatcg cgaacgttgt tttcgtaccg gtcatggacg caaagtggtt
    16981 agccagataa gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga
    17041 cagagtaaac aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc
    17101 acgagaaatg atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata
    17161 ctcttcacgt ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac
    17221 catgatggaa atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag
    17281 cgaaccgagg tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga
    17341 aatcagcacc aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag
    17401 atccttacca aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc
    17461 gcgattcagc ggtgcaaatc cgagctggtt ttcccagaac gtcataaagg tattggtaat
    17521 agtaacgcca taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac
    17581 cacgccgcca gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt
    17641 agagccagaa agacacagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc
    17701 caataccaac atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa
    17761 cagcaccccg gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt
    17821 acgccaggac gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta
    17881 tcctcaacga attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc
    17941 gatattgccg ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt
    18001 accgcttaat aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg
    18061 ctgaattaag gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc
    18121 gactaattca cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc
    18181 cgccagggtg ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat
    18241 gccgtgaacg ttttctgact gtacgccaat aacacgaatg gtcgggttaa tagatttaat
    18301 tgccaccgca ataccagcaa ttaaaccacc accaccaatt ggcacaatca cgttatcgac
    18361 atcatagaga tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg
    18421 atcatcgtaa ggtgggataa aaatacgacc ttccatttcg acaatttcgc tcactttagc
    18481 gatagtgtcg ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc
    18541 cgctactttg gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat
    18601 cgcgcaggag agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt
    18661 gcgtttttcc gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga
    18721 acccgtacgc tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa
    18781 atagttggag cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc
    18841 aataatatca tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg
    18901 cctgaattaa atatgtaggt aaccgtaacc gacacctgca aaacagacag gtggattatt
    18961 tatgtttaaa taaaaacaaa caactaacca acttctatta attgccttcg tctacaccca
    19021 ttataagatg aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga
    19081 taatttttcg accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata
    19141 gtaataaatt gattagaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa
    19201 tcagcattga gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca
    19261 atactgatgc catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt
    19321 tgtggcaaca cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt
    19381 cgggacttac tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc
    19441 tgaagcttca tttctgcact taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt
    19501 gccggtaaga acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc
    19561 tctttgaact tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca
    19621 aatgagactt ccaccaccgc ttcagaagac ataccgctta tctcattaac catatttttc
    19681 atttcacggg taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt
    19741 acaccggtat ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg
    19801 attttactga cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca
    19861 cttctaatga cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc
    19921 ataacgacct acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaataaaca
    19981 ggatatgtgc gaccactcac aaattaactt tcattacttt ccaaggtatc gttattacaa
    20041 ttaaatatct ttaaatatca acaagttaaa gcataaaaat cagcataaac cctgattttt
    20101 atcaaaaaaa aatgacgggg ataaccatat tttatggcga taacatcatt cgttatgtgg
    20161 ttaatacaaa aaaggggctg agaccatatt tcaagcaatt accggataat taccaggcaa
    20221 aatttgagtt aaatttaatg agcaagttct caaattttat tataaataaa ccattttcag
    20281 cgataaatac tgcggcacgt cacatttttt cacgctattt gttggagaac aaacatttat
    20341 tttatcaata ttttaaaatt tcgaatacag gtattgatca tctcgaacaa ttgattaacg
    20401 tcaacttttt ctcttctgac aggacgtcat tttgtgaatg caatcgtttt ccataaattc
    20461 ttctcccctc ataggcgacg aatagcattt tgtgttgagg atcacaaaac gaataattgc
    20521 tggtcgccgc gataaggtca gacgaagtca acaaggggga ttttcactgt gctttacatc
    20581 agcgtaggac acagaatgtt gcttcgttta ctgtaacgcc gggtaaatga gcgttttttg
    20641 atagtgcgta agtgtcgtgc gaggaaaata agcatctatt attgtttgtc atgacgacaa
    20701 ggcaggcatc aatgaaaggc ttcccaattg cacatatttt tcacccttca atcccgccaa
    20761 tgcacgcagt ggttaacaat cacaatagaa atattgatta ttggacggta aaaagaaagt
    20821 ttgcagaaat tgtctccacc aatgacgtta ataaaattta cagtataagt aatgaactcc
    20881 ggagagtatt atctgcaatt actgcattga atttctatca aggcgatgtt ccttctgtca
    20941 tgatccgaat ccaaccggaa aatatgagtc cattcatcat agatatttct acaggagaac
    21001 atgatgatta tatcatacaa acattagatg taggcacttt tgcacctttt ggtgaacaat
    21061 gtacttgctc agccgtcaat aaaaaagagc tggaatgtat taaggagacg atttctaagt
    21121 attgcgcaaa gttcacccaa aaagaagcca tcttaacacc ccctaggcac tttaataaaa
    21181 cctgcattac ttcagattgt tggcaaattc tctttttttc acccgatcat tttaataatg
    21241 atttttattg attatttata aaggataaaa taatgttccc tgtttcatca attggtaatg
    21301 atattagtag tgatttagtc cgtcgaaaaa tgaatgacct tcctgaaagc cccataggaa
    21361 ataacctcga agcactagca ccaggtatag aaaaactaaa acagacctct attcaaatgg
    21421 tcactttact taacgcgtta caaccaagtg gaaaatgcat tatcactggt gattttcaaa
    21481 aagaattagc gtacttacaa aatgtaattc tttataatga ctcgtctctt cgtctggatt
    21541 ttttaggtta taacacccaa attattcaac gatcggacaa tacctgcgaa cttaccatta
    21601 atgaaccgtt aaaaaactag gaaatatcta caggcaatat caatgttaat tgcccattaa
    21661 aagatattta caatgaaatc aggaggttga acgtaatctt tagttgtggg actggagata
    21721 tagttgatct atcctctctg gacttacgta atgtcgattt agattattat gatttcacag
    21781 ataaacatat ggctaatact attctaaatc attttaaatt ggattcaaca aattttacta
    21841 atgccaacat gtttcaggtt aattttgtta gttcaacaca aaacgccaca atctcctggg
    21901 attatttact aaaaataacg cctgttttaa taagcattag cgatatgtat tctgaagaaa
    21961 aaatcaagtt tgtcgaaagt tgtttaaatg agcttggaga cattaccgaa gaacaattaa
    22021 aaattatgag atttgcaatt ataaaatcta taccaagggc aactcttaca gataaattag
    22081 aaaatgaatt aacaaaagaa atatataaaa gctcatcgaa aatcaacaat tgcttgaaca
    22141 gaattaaatt accagagatg aaagaattct catcaaaaaa aatatatgat tacatcgata
    22201 caatcattga agattatgaa aatatcaaag aaaatgctta tctggtcgtc ccccaaatta
    22261 attatactat ggatttaaac atagaagact ctagctcaga agagttaata ccctcgagaa
    22321 agacgaaaat tctccggaca atggctttga ggtcggggaa tataacacat atgaagcata
    22381 taactcagag aatcaatatt ttaccagaga ggactatacg tataattacg accttttaaa
    22441 tgcaatatag acttaataac ttcttacaaa gtctattatt aataccgtag atattcatct
    22501 taccctgtgc accaaaagca aaaacttatc gacgcgggtt tgaaactgat ggcaaccgca
    22561 aaaatgcctg atgcgctacg cttatcaggc ctacgccatc tctgcaatat attgaatttg
    22621 cgtgcttttg taggcaggat aaggcgttca cgcagcatcc ggcatgaata aagcgcactc
    22681 aacaatctga aacccgccgg aacggtttat tacgtacatc aggtgaaact gaccgataag
    22741 ccactttctt ttgggtatag tgtcgtggac agtcattcat ctttctgccc ctccaaaagc
    22801 aaaaacccgc cgaagcgggt ttttacgta
//
LOCUS       DAOFMX010000064        22402 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.064, whole genome shotgun sequence.
ACCESSION   DAOFMX010000064 DAOFMX010000000
VERSION     DAOFMX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.064"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            83..2668
                     /locus_tag="QBA20_002854"
     CDS             83..2668
                     /locus_tag="QBA20_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDQ6293316.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRS
                     SGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSL
                     KGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEAD
                     GSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTW
                     YGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLN
                     QLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGK
                     LQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRG
                     QPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETL
                     LDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGS
                     LVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRI
                     ALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIAT
                     LDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRM
                     DAICR"
     gene            2659..3642
                     /locus_tag="QBA20_002855"
     CDS             2659..3642
                     /locus_tag="QBA20_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6293317.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            3740..4282
                     /locus_tag="QBA20_002856"
     CDS             3740..4282
                     /locus_tag="QBA20_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6293318.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4290..4805
                     /locus_tag="QBA20_002857"
     CDS             4290..4805
                     /locus_tag="QBA20_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6293319.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTGFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4771..5509
                     /locus_tag="QBA20_002858"
                     /pseudo
     CDS             4771..5509
                     /locus_tag="QBA20_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            5620..6630
                     /gene="pyrD"
                     /locus_tag="QBA20_002859"
     CDS             5620..6630
                     /gene="pyrD"
                     /locus_tag="QBA20_002859"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDQ6293320.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6804..7346
                     /gene="zapC"
                     /locus_tag="QBA20_002860"
     CDS             6804..7346
                     /gene="zapC"
                     /locus_tag="QBA20_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDQ6293321.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7343..8452)
                     /gene="ycbX"
                     /locus_tag="QBA20_002861"
     CDS             complement(7343..8452)
                     /gene="ycbX"
                     /locus_tag="QBA20_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDQ6293322.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8696..10804
                     /gene="rlmKL"
                     /locus_tag="QBA20_002862"
     CDS             8696..10804
                     /gene="rlmKL"
                     /locus_tag="QBA20_002862"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDQ6293323.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10816..12723
                     /locus_tag="QBA20_002863"
     CDS             10816..12723
                     /locus_tag="QBA20_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6293324.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12853..14106
                     /gene="pqiA"
                     /locus_tag="QBA20_002864"
     CDS             12853..14106
                     /gene="pqiA"
                     /locus_tag="QBA20_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDQ6293325.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14111..15751
                     /gene="pqiB"
                     /locus_tag="QBA20_002865"
     CDS             14111..15751
                     /gene="pqiB"
                     /locus_tag="QBA20_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDQ6293326.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15748..16311
                     /gene="pqiC"
                     /locus_tag="QBA20_002866"
     CDS             15748..16311
                     /gene="pqiC"
                     /locus_tag="QBA20_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDQ6293327.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16567..16734
                     /gene="rmf"
                     /locus_tag="QBA20_002867"
     CDS             16567..16734
                     /gene="rmf"
                     /locus_tag="QBA20_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDQ6293328.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16804..17322)
                     /gene="fabA"
                     /locus_tag="QBA20_002868"
     CDS             complement(16804..17322)
                     /gene="fabA"
                     /locus_tag="QBA20_002868"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDQ6293329.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17391..19151)
                     /locus_tag="QBA20_002869"
     CDS             complement(17391..19151)
                     /locus_tag="QBA20_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDQ6293330.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19336..19788
                     /gene="matP"
                     /locus_tag="QBA20_002870"
     CDS             19336..19788
                     /gene="matP"
                     /locus_tag="QBA20_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDQ6293331.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19864..20910)
                     /gene="ompA"
                     /locus_tag="QBA20_002871"
     CDS             complement(19864..20910)
                     /gene="ompA"
                     /locus_tag="QBA20_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDQ6293332.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21267..21776)
                     /gene="sulA"
                     /locus_tag="QBA20_002872"
     CDS             complement(21267..21776)
                     /gene="sulA"
                     /locus_tag="QBA20_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDQ6293333.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21995..>22402
                     /gene="sxy"
                     /locus_tag="QBA20_002873"
     CDS             21995..>22402
                     /gene="sxy"
                     /locus_tag="QBA20_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDQ6293334.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5720 a         5254 c         5873 g         5555 t
ORIGIN      
        1 gtatcgaacc atcaatgact acggggcgga aacgccgcaa ctcaactgta aatcgtaagc
       61 cgtcttcagt taagagagcg agatgtatag aactcatcga caacacagcc tgttaagctc
      121 tggtggagtg ccatcgttta ttggtgggct ggtggtgttt gtgtcggcag cgttcaatgc
      181 acaagctgaa acctggttcg atcctgcttt tttcaaagat gatccctcaa tggtggccga
      241 tttgtctcgt ttcgaaaaag gacaaaaaat aacgccaggg gtttatcgtg tcgatattgt
      301 tctgaatcag acaattgtag atacgcgcaa cgtcaatttt gttgagataa cgccagagaa
      361 ggggattgcc gcctgtttga cgactgaaag cctggatgca atgggtgtta atactgatgc
      421 gtttccggct tttaaacaac tggacaaaca agtgtgtgtg ccattggcgg agattattcc
      481 ggatgccagc gtaacgttta atgtgaataa actccgtctg gaaatttcag taccgcaaat
      541 cgccatcaaa agtaatgccc gtggttatgt cccccctgaa cgttgggatg aagggatcaa
      601 cgcgctattt agcggggcta acagtattca tagcagcgca gacagtgatt ctggcgacag
      661 ctattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc gcaacaattc
      721 aacatggagt cgcagtagtg gccaaaccgc agaatggaag aatctcagca gctatttgca
      781 gcgggcggtt attccactaa aaggcgaact gaccgtaggt gatgattata ctgcaggcga
      841 ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca acatgctgcc
      901 agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca atgcccagat
      961 aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg gtgcttttga
     1021 aattagtgat ctctattcca cgtcgtcgag cggtgatttg ttagttgaaa tcaaagaagc
     1081 ggacggcagc gtcaatagct acagcgtacc gttttccagc gtgccattac tccagcgtca
     1141 ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta atgaacagca
     1201 ggagagcaaa tttgcccaga ccacgttgca gtggggcgga ccgtggggaa cgacatggta
     1261 tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg gatttaacct
     1321 tggcgatttc ggagcaattt cgttcgatgc gacccaggcg aagagtacgc tggcagacca
     1381 aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca accaattggg
     1441 cactaatttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca ccctttccga
     1501 caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg aagatacgcc
     1561 gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc aggtcaatat
     1621 ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt ggtagccagc aaacttactg
     1681 gcataccgat caacaggatc ggctattaca gtttggctac aacacgcaaa ttaaagatct
     1741 ctcgctgggg gtttcctgga actacagtaa gtcccgtggt caacctgatg ctgatcaggt
     1801 gtttgcacta aatttttccc tgccgctcaa tctgttgctc cccagaagta atgatagcta
     1861 taccaggaaa aaaaattacg cctggatgac ctctaacacc agtatcgata acgaagggca
     1921 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     1981 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     2041 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     2101 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     2161 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     2221 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     2281 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     2341 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     2401 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     2461 tggcgcgata gcgacgctgg acggcataca gactaatagc ggcattattg atgatgatgg
     2521 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     2581 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     2641 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     2701 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tatcgggcag
     2761 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aactctggat
     2821 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     2881 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     2941 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     3001 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     3061 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     3121 ggccaggggg ctagcggtaa tagcggtagc ttttcactga aaatattgca gccgttcgtt
     3181 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     3241 gcaggtgatt cctgcacgac tactggtaca ccggttttag tgtattacct gtctggtacg
     3301 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt tgatctgggc
     3361 gacgtatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     3421 gaacttgcaa ttccggtcag gtgtaacacg ggaaacgcag ggttagttaa cgtcaatctg
     3481 agtctgacgg caacctcaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     3541 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tgggacacta
     3601 ccgctctcta ttcctgatga tgcagacagt atccgcgaat gaatgtctat ccagtcagca
     3661 cgacaggcgt atcaccagaa accgggcgat ttgaagccac ggcaacggtg agaataaatt
     3721 ttgattaacg cgtgaacgta tgaagaaaaa aacgatatat cagtgcgtta ttttgttctt
     3781 tagccttctt aacatccatg tcgggatggc tgggcctgaa caagtcagta tgcatattta
     3841 tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac aaaatattca
     3901 tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca ctgctgctga
     3961 tttgaatatt gatatcaccg gttgtgccgc tggtattact ggcgcggacg tcctttttag
     4021 cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag gcggaagcgg
     4081 tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc aagaaatccc
     4141 gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac tcaaatatca
     4201 acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg cggttctcta
     4261 ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg attctgttct
     4321 tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc aacattagcg
     4381 gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc aatgtgcagt
     4441 taggcgatgt ctcgctgaca ggtttttttc atgggaatta tggcccggag aaatctttca
     4501 tcattaattt gcaggattgc ggaacagatg tcagcacggt tgatgtcacc ttttcaggaa
     4561 cccccgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat gcagccagtg
     4621 ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac caggcgagta
     4681 aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa ttgcgacctg
     4741 tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta cttcattatg
     4801 actaatacgt ggaacagatt agcgctcttg attttcgccg ttttatcgct gctggtggcg
     4861 ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc ggcagacaga
     4921 gaatcgatat ctattttact gactaatacc agtcaggaat cttggcttat taacagcaaa
     4981 atcaatcgcc caacgcgttg ggccgggggg ggaagcgtcg acagtgccag cactattact
     5041 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     5101 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     5161 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     5221 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     5281 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     5341 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagtcgtgc cgcctaaaag
     5401 ccagcgtcag acaaactggt gtcaggtaat agcaccgtgt catgtcgcct ggcggacgat
     5461 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttctg
     5521 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     5581 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     5641 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     5701 cgcattaccg gaacaccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     5761 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     5821 ggggagtgca ttgatgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     5881 acgccacgtc cacagcctgg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     5941 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     6001 aaaaaggccc attatgacgg cgtcttgggt attaatatcg gcaaaaataa agatacgcca
     6061 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     6121 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     6181 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     6241 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agacgaattg
     6301 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     6361 actctcgatc gttctctggt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     6421 ggtcgtccgc ttcagttaaa aagcacagaa attattcgcc gcttgtcact agaattaaac
     6481 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     6541 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     6601 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     6661 atcagggctt tattttcagc cctggttgtt ttatattcat cgttgttgct tatttagaca
     6721 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     6781 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     6841 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     6901 gttttgcgcg caaaatgttg actccagatg ccttttctcc cgcaggcttt tgtgttgatg
     6961 atgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagacc
     7021 aaaaagccga actggtgctg aatgcactgg tagcaattcg ttatctcaaa ccgcaaatgc
     7081 cgaaaagctg gcattttgtc tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     7141 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     7201 aaaatgccgc gctatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     7261 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtg aatgttgaca
     7321 gcttcagcct cgaacaggca gtctaacgcg tcagcttaag ggcagtcttc ggtacacagc
     7381 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc gtgacttcgc
     7441 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     7501 gaattccctg attttctaat tgctccagca acacttgttg gttatttcca cgaaatgcct
     7561 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     7621 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     7681 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     7741 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     7801 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     7861 acaccacatc accaatgcga atcactttcc agctatcttc ttcccaggct gacgcgccgg
     7921 aaaccaccag attggtgcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     7981 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     8041 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     8101 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     8161 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     8221 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     8281 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     8341 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     8401 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     8461 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     8521 aacaattttc ttagcgcctg tcccgttagg ctattttatt tgtcagtttg gccctgggtt
     8581 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctct ccatgtccag
     8641 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
     8701 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
     8761 tctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
     8821 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
     8881 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
     8941 agagatgttt aatcctggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
     9001 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
     9061 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
     9121 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
     9181 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
     9241 gatgcgatcc ggctggcagt caggaacacc gctgctcgat ccgatgtgtg gttccggtac
     9301 gttgctgatt gaagcggcga tgctggcgac cgatcgcgca ccaggcttgc accgtgggcg
     9361 ttggggcttt agcggctggg cgcagcatga tgaagctatc tggcaggaag tgaaagcgga
     9421 agcgcaaact cgcgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
     9481 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
     9541 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
     9601 gtacggtaca gtgttgagca acccgccata cggtgaacgt ctggacagcg aaccggcgct
     9661 gattgcgctg catagtctgc tgggccgaat catgaaaaac cagttcggtg gctggaatct
     9721 ctctttgttt agtgcctcgc cggatctgct aagctgcctg cagctgcgtg cagacaaaca
     9781 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
     9841 cacaccagac agtaaaccgg caatggtagc ggaagattac gccaaccgcc tgcgtaagaa
     9901 cctcaaaaaa ttcgagaagt gggcacgcca ggaagggatt gaatgttacc gcctgtatga
     9961 cgccgatctg ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    10021 ggagtatgcg ccgccaaaaa ctattgatgc gcacaaagcg cgtcagcgtc tgttcgatat
    10081 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    10141 tgaacgccat aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    10201 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    10261 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    10321 cctcaacctg ttttcttata ccggcagcgc caccgtgcat gcgggattag gcggtgcacg
    10381 cagcaccacc accgtggata tgtcgcgtac ttatctggaa tgggcagaac gcaacctgcg
    10441 tctgaatggc ctgactaggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    10501 gcgtgaggca aatgaacagt tcgatctgat ctttatcgat ccgccaacct tctctaactc
    10561 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat ctggcgctga tgaaagattt
    10621 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    10681 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    10741 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    10801 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    10861 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    10921 ggccgcaacg gcgcaggaaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    10981 gatgacggtc gcattattta tgagcaagat ttgattgtag ctcgtctgca acaggatcca
    11041 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    11101 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    11161 aatctcaacg aactggcgaa ggttcaggaa cagctggatc accacaacct gtggcagctg
    11221 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaacgt tgcgctgtcg
    11281 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagcaatccg
    11341 cgcgtattgc tgctggacga accgactaac catctggata tcgaaaccat cgactggctg
    11401 gaggggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    11461 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    11521 gggaattacg accagtacct gcaggaaaaa gaagaagccc tgcgcgtgga agaactacaa
    11581 aatgccgagt tcgatctcaa actggcgcag gaagaagtgt ggattcgcca ggggatcaaa
    11641 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcgatgcg tcgcgaacgt
    11701 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    11761 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttaacggtaa gcaactggtg
    11821 aaagattttt ctgcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    11881 tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc ttcaagcgga cagcgggcgt
    11941 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    12001 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaatggc
    12061 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    12121 acgccagtac gtgcgttttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    12181 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    12241 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    12301 gatcgtcagt ttgtcgataa caccgttaca gaatgctgga tcttcgaagg cggcggtaaa
    12361 attggtcgtt atgttggcgg ttatcatgat gcccgtggtc agcaagaaca atatgtggca
    12421 ctcaaacagc ctgcggtgaa aaaaagcgaa gaagccgccg cgccaaaagc agaaactgta
    12481 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    12541 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaagtggc ggatgcttcc
    12601 ttcttcagtc agccgcatga gcagacgcaa aaagtgcttg ctgatatggc tgctgcagag
    12661 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    12721 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    12781 ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat agcgcatcat
    12841 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    12901 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    12961 ggcacaacgt taactgtggc gtgggatgcc cctcggcagc gtcccaccgc ctatgcgttg
    13021 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    13081 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    13141 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    13201 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    13261 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc gggtgtgctg
    13321 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    13381 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    13441 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    13501 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    13561 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct gcagtggaca
    13621 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    13681 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    13741 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    13801 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    13861 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    13921 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    13981 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    14041 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    14101 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    14161 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    14221 ttacagccat cagggaccgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    14281 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    14341 ggctgatgat ttgacgcacg ttgaaatcaa agcgcggctg aattccggta tggaaaaatt
    14401 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    14461 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    14521 caaaatggat aaatacgatt tgctggactc accaccgttg gccccgcctg atgcgaaagg
    14581 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    14641 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    14701 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    14761 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    14821 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    14881 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    14941 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    15001 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    15061 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    15121 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    15181 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    15241 ggtcactggt gcactgtatg ttgatctcga tttctatcca aatacgcctg caataaccgg
    15301 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggct tggcgcaaat
    15361 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    15421 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    15481 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    15541 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    15601 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    15661 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    15721 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    15781 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    15841 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    15901 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    15961 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacagttgcg caacaccctg
    16021 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    16081 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    16141 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    16201 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtgct ggccggtgtc
    16261 tggagtcagg aagccgcttc tattgcacaa gagataaagc gtctacctta attataaaga
    16321 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    16381 ggaaaaattt gttcctcttc acattttttg tacaaccgac atgcccgtgt agctcacaaa
    16441 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    16501 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    16561 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    16621 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgag tcaaaggtca
    16681 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    16741 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatga
    16801 taatcagaag gcagacgtat cctggaacag accgactttc aggtcgctgg cggtatagat
    16861 cagacgacaa tcaaccagca cttcgccatc tgccagaccc ataatcagac gacgattaac
    16921 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    16981 gaatttcact tcgccaacgc ccagcgcgcg gcctttacct tcgccgccca gccagccgag
    17041 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    17101 gccaatgaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    17161 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ctatcatcag
    17221 catgttcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgt gaccagaggc
    17281 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    17341 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    17401 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    17461 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    17521 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    17581 ttgccttctt ctaccgcttt caccagttca ctgtgaagac ttaaatggcg aacgttagca
    17641 gtggggataa tgacaccttg tttccccgtt aactcacgtt gctggcaaat agcaaagaag
    17701 ccttcgattt tctcatttaa accaccgacc ggctggacgc ggccgaactg atcgactgaa
    17761 cctgtgatag cgatactctg attcactggc acatcagcga gggcgcttat cagggcgcag
    17821 agttcagcca tcgaggcact atcgccatcc acttcactgt atgactgctc aaatgtcagc
    17881 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcctgcatg
    17941 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    18001 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    18061 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    18121 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    18181 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    18241 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    18301 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca agcatccgct
    18361 cccggggcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactggctt
    18421 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    18481 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    18541 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    18601 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    18661 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    18721 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    18781 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    18841 gcgtgacgta agcggatgga gtggccggaa acctcatagt gatcgcccac cagttggcct
    18901 gcatcgcttt gtagcgtacg cgcggcatcg gcaataagat tcagatactc agactcttcc
    18961 ggggccttag ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    19021 aattgcagtc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    19081 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    19141 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    19201 gcactacagc acacgtagaa agcaccgaca atactcctgg ctgggcgtta aagctcacag
    19261 gatggagatt cttttcttca ctggcctaaa aagctgatat tctgtaaaga gttacacggt
    19321 aacattgaga tcgctatgaa atatcaacaa cttgaaaatc ttgaaagcgg ttggaaatgg
    19381 aagtatctgg tgaagaagca tcgcgaaggg gagttaatta cccgttacat agaagccagt
    19441 gccgcccagg aagccgtcga tgtgttgctc tcgctcgaaa atgaacccgt gctggtaaat
    19501 ggctggattg acaagcatat gaatccggaa ctggtcaatc gcatgaagca gaccattcgg
    19561 gccagacgga agcgccattt taatgcggaa caccagcata cgcgcaaaaa atcgatcgat
    19621 ctggaattta ttgtctggca acgtctggct ggtcttgcac agcgtcgcgg taaaacgctt
    19681 tctgaaacga ttgttcagct gattgaagat gcggaaaaca aagagaaata cgcgaataaa
    19741 atgtcttctc ttaagcagga tctgcaggca ttgctgggta aggaataact gacgaaagtc
    19801 agttcaattt actaaaggca aaaaaaaccc cgcagcagcg gggtttttct accagacgat
    19861 aacttaagcc tgcggctgag ttacaacgtc tttgatacct ttaacttcga tctctacacg
    19921 acgatccgga gccaagcagt cgatcagtgc agcacgctgt ttcacgttgt cacaggtgtt
    19981 gccagtaacc gggttggatt cgcccatacc acgtgcggag atcttgtctg ccgggatacc
    20041 tttggagatc aggtaatcaa caacagactg agcacggcgc tcggacagac cctggttgta
    20101 agcgtcagaa ccgatgcggt cagtgtaacc cagaacaact acggaaccgt ctttcggatc
    20161 taggttgctc agctggctgt acagctgatc cagagcagcc tgaccttccg gtttcagggt
    20221 tgctttgttg aagttgaaca gaacgtcaga cttcagagtg aagtgcttgg tctgtacttc
    20281 cggtgccgga gctggagcaa ctaccggagc tgcttcgccc tgaccgaaac ggtaggaaac
    20341 acccaggctc agcagaccgt tgtccggacg agtaccgatg gtgtttgcgt caccgatgtt
    20401 gttggtccac tggtattcca gacgggtagc gatttcagga gtgatcgcgt actcaacacc
    20461 acctgcgaaa accggagaaa cgccagtgtc gtggtcttta aaggatgcgc caccaggtac
    20521 gttagccttg gtgtctgcac gccataccat accacccaga cgagtgtaga tgtccagatc
    20581 gtcagtgatt gggtaaccca gtttagcggt cagctgaacg ccctgagctt tgtatgcgcc
    20641 gttgatgttg tcgcctttgt acggcatacg acctaaccag tcgtaaccca tttcaaaacc
    20701 aacatacggg ttaacctggt aaccaccaaa agcacctgca cccagttggt tttcgtgggt
    20761 cggaccattg ttaggaataa aaccagtgtc atggtactgg gaccagccca gtttagcacc
    20821 agtgtaccag gtgttatctt tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc
    20881 cactgcaatc gcgatagctg tctttttcat tttttgcgcc tcgttatcat ccaaaatacg
    20941 ccatgaatat ctccaacgag ataacacggt taaatccttc accgggggat ctgctcaata
    21001 ttaactctac cgatatcttc ggcttatgcc gagcacccct ggcgatgtaa agtctacaac
    21061 gtagttgaaa acttacaagt gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa
    21121 ggtatgttta atctttttgt cagcgacaat ttacagaaaa aaaacgcgga aaccgcttca
    21181 gacaagcctt cgcaaggaaa attagtcacg actgaaagca ttggctgggc gacaaaaaaa
    21241 agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat ttttagcccg
    21301 gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa ccccatagcg
    21361 ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa atcatctgtc
    21421 aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat tgactccact
    21481 gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag cccagatgcc
    21541 tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca gcgcgattgc
    21601 tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg ctgatcttcg
    21661 cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac acgcgcaatt
    21721 ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt gtacataatc
    21781 aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca acaaagatca
    21841 accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa acataaatta
    21901 atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt taatgtgaat
    21961 cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct ataagcggat
    22021 ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat cattgtttgg
    22081 cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg gtgagttgta
    22141 tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg tctggctgac
    22201 atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg aaagtctatg
    22261 gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga
    22321 gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt cttttcatct
    22381 ggaagcgatt ctcggggagg tg
//
LOCUS       DAOFMX010000065        22345 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.065, whole genome shotgun sequence.
ACCESSION   DAOFMX010000065 DAOFMX010000000
VERSION     DAOFMX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.065"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..1028)
                     /locus_tag="QBA20_002874"
     CDS             complement(<1..1028)
                     /locus_tag="QBA20_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6293335.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1130..2110)
                     /locus_tag="QBA20_002875"
     CDS             complement(1130..2110)
                     /locus_tag="QBA20_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDQ6293336.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2118..2828)
                     /locus_tag="QBA20_002876"
     CDS             complement(2118..2828)
                     /locus_tag="QBA20_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDQ6293337.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3045..4868)
                     /gene="typA"
                     /locus_tag="QBA20_002877"
     CDS             complement(3045..4868)
                     /gene="typA"
                     /locus_tag="QBA20_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDQ6293338.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PVVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5241..6650
                     /gene="glnA"
                     /locus_tag="QBA20_002878"
     CDS             5241..6650
                     /gene="glnA"
                     /locus_tag="QBA20_002878"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDQ6293339.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6823..7872
                     /gene="glnL"
                     /locus_tag="QBA20_002879"
     CDS             6823..7872
                     /gene="glnL"
                     /locus_tag="QBA20_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDQ6293340.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7884..9293
                     /gene="glnG"
                     /locus_tag="QBA20_002880"
     CDS             7884..9293
                     /gene="glnG"
                     /locus_tag="QBA20_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDQ6293341.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9405..9515
                     /locus_tag="QBA20_002881"
     CDS             9405..9515
                     /locus_tag="QBA20_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDQ6293342.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="QBA20_002882"
     CDS             complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="QBA20_002882"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDQ6293343.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="QBA20_002883"
     CDS             complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="QBA20_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDQ6293344.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12397..13029
                     /locus_tag="QBA20_002884"
     CDS             12397..13029
                     /locus_tag="QBA20_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDQ6293345.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13411..16197)
                     /gene="polA"
                     /locus_tag="QBA20_002885"
     CDS             complement(13411..16197)
                     /gene="polA"
                     /locus_tag="QBA20_002885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDQ6293346.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16561..17493
                     /locus_tag="QBA20_002886"
     CDS             16561..17493
                     /locus_tag="QBA20_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDQ6293347.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17534..18964)
                     /locus_tag="QBA20_002887"
     CDS             complement(17534..18964)
                     /locus_tag="QBA20_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDQ6293348.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="QBA20_002888"
     CDS             complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="QBA20_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDQ6293349.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="QBA20_002889"
     CDS             complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="QBA20_002889"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDQ6293350.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20825..21094)
                     /locus_tag="QBA20_002890"
     CDS             complement(20825..21094)
                     /locus_tag="QBA20_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDQ6293351.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21164..21748
                     /gene="mobA"
                     /locus_tag="QBA20_002891"
     CDS             21164..21748
                     /gene="mobA"
                     /locus_tag="QBA20_002891"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDQ6293352.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNHAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21730..22257
                     /gene="mobB"
                     /locus_tag="QBA20_002892"
     CDS             21730..22257
                     /gene="mobB"
                     /locus_tag="QBA20_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDQ6293353.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5525 a         5609 c         5349 g         5862 t
ORIGIN      
        1 tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag
       61 cccagcgtac atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac
      121 agtacgagcg cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc
      181 aggatcttat ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg
      241 ttaatgatgc cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat
      301 ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt gaaagcgata
      361 agccagatgg ttttgtctct cagcaccgag gtcatactgg tgttttgcga tgctccgtct
      421 tcttttttca cctcaacgga cggtgcctct tctttgtcat tcaggacaaa gaaaataata
      481 atgcctacgg caatcacaat aagggaatag aaccagatgc ctgctttaaa acctaataag
      541 ccactgccaa accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg
      601 ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga
      661 cttaccgact tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc
      721 cagacaaaga ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct
      781 gtcgcaatga gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata
      841 gacagaaaaa agcccactgt ggtgacaaat gagtttaccg acatagcatt accaatttga
      901 ccattggtca aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt
      961 aatttataaa ttgtaccgcc acacagtgtt aatagactaa ataacgccca tttctttttc
     1021 gtgagcataa catcccctaa gcgattttta aaatcactgt tttaaaggca ataagatacc
     1081 gaccattcat atggaatggt tttattaact gcgtatgagt taaaacttat tagtggttca
     1141 gaagatggtt tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat
     1201 taattgcatc ttctttttct ttacgtaacc gcgcatccag taaatgattt tctggtaggg
     1261 gtgtttcact catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga
     1321 acgccgacgc atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat
     1381 catcgccaag gcagataagg tgcgttaata acgctttgaa tggcatatca ccctgacctg
     1441 aaatacaggc tttatgaccc aggccaccct gttctttaac gatcaaggca tctttaatat
     1501 ggacctgggt aatatgcggt gccatcgttt ttaaggcgtc gatgggatgc tcatttgcat
     1561 tgatcatgtt agcaaaatca aacagtaagg aaagagactc catctcactt tctttgacca
     1621 gcgacaccag ctcatgactt tttaaatctt catgctgctc aatcgtaaaa gtcaggccgc
     1681 tgtcctgata cgtttcccgt acataggcaa tgtcgttagc gataatcgat aatacgtcgc
     1741 gcaaattacc ttcataacgt ggataaaaac gtacggacgt tgccccagtt ttcaacgcga
     1801 tggcgacggc ttcgtcaata gatgccttat ctgaagcgct ggtttcaata tgaatatcaa
     1861 ggttcagacg gcgggcttta tccccaaagg cagagagttc tttatcgtcc atattaccaa
     1921 gagaaaaacg ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcacttg
     1981 cgatatcaag taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat
     2041 aagcatgtaa ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta
     2101 ttgtcaccat aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat
     2161 tcaatatcta tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc
     2221 gctggcatac ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt
     2281 ttgaaaatca atttttttac taccgtctct ttttggtagc acttttccca gactgcagaa
     2341 aatgagtggt tttcaagttg ttcaataaaa cccggggcaa cttcaggatt aatatatgtt
     2401 tcgtgataga aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca
     2461 gttgatggcg tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt
     2521 ttctcggtaa acccccaaga aggctctcgc ccttgctcaa ttgcggcccg ctgaaagctc
     2581 gccgacagct ccggactgta attaaatcgc ggctgggtca cgaaccaccc cttacgatcc
     2641 ttacggaaga ttttggattc tgcctcgagg ttcaacaacg cctggcgcag cgtcatacgt
     2701 ttaatgccca gcagttctcc cagctctctt tctgaaggga gttttccacc cggcgtggtg
     2761 atgccatctt tcaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattc
     2821 tccgccattg tttaatcctt ttggtttaaa ccagatgaag catcttacgc ttacgccaaa
     2881 aaagaaagca attaacggtg tatgactttg cagataattg tgacaaagct ctctattgac
     2941 gtaatcacag acttataagg gagatactcg cattggtgtg cgctcacgac aggtaaaaaa
     3001 aaacctgcca tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt
     3061 gcgcggttgg cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag
     3121 gtcggagtca cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc
     3181 atgcggatag gcggaaccag aacaacagct tcgtcagtac cggaagcacg catgttggtc
     3241 agtttcttac cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata
     3301 atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga
     3361 ccgaacagcg cgaacgcgac agctttaccc tgaccgttag agatcagtac accgttctga
     3421 cgctgaccca cttcgcccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga
     3481 ccagtaccgg aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg
     3541 atcacgtagt cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct
     3601 ttacgttcgc ccagcgcctg catcacagaa ccctgatgct gttcttcaac gtccagcgtc
     3661 acgttttcat acggctcttg tttacgaccg tctatttcac ggaagattac tttcggacgg
     3721 gataccgcca gttcgaaacc ttcacgacgc atgttttcga taagaacaga gaggtgcagt
     3781 tcaccacgac cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca
     3841 acgttgtgta ccagttcttt gttcagacga tcgaggatct gacgagacgt tacgaactta
     3901 ccttctttac cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca
     3961 tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg
     4021 ttcagttcgc ccaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg
     4081 atacgttcca gaccgaggtg gcccagcact ttaccgactt tcgcgttacg ggttttgcct
     4141 tcgctatcga tgatagtaac ctgctggttc ggcttcactt taccacgctt gatgcggcca
     4201 atgccgataa cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga
     4261 ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa tcgcctgata cagcggggtc
     4321 atgtcttccg ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa
     4381 acgaccggga agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc
     4441 tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc
     4501 ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg
     4561 tcaaatgcgt caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca
     4621 ccaccgaagt cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg
     4681 atagcggtgt ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc
     4741 atcacgcgct cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg
     4801 tctaccaggg tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt
     4861 tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc
     4921 actacaaaac aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat
     4981 cgctttcacg gagcataaaa agggttatcc aaaggtcatt gcaccaatat ggtgcttaat
     5041 gtttccattg aagcactata ttggtgcaac attcacatca tggtgcagcc cttttgcacg
     5101 atgttaagca tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta
     5161 tcttttttac ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgacaatgac
     5221 caatccagga gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg
     5281 aagtgaagtt tgttgatttg cgcttcaccg ataccaaagg taaagaacaa cacgtcacta
     5341 tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct
     5401 cgattggcgg ctggaaaggc attaacgaat ccgacatggt gctgatgcca gacgcatcca
     5461 ctgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg
     5521 aacctggcac cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag
     5581 attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct
     5641 tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg
     5701 atatcgaagg cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc
     5761 cggcagtgaa aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt
     5821 ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag
     5881 tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg
     5941 acgaaattca gatctacaaa tatgtcgtgc ataacgtagc gcaccgcttc ggtaaaaccg
     6001 cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt
     6061 ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc
     6121 aggcgctgta ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa
     6181 acccgaccac caactcttat aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg
     6241 cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag
     6301 cacgtcgtat cgaagtacgt ttcccagatc cggcagctaa cccgtacctg tgctttgctg
     6361 ccctgctgat ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg
     6421 acaaaaacct gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct
     6481 ctctggaaga agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg
     6541 tgttcacgga tgaagcgatc gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg
     6601 tgcgtatgac tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt
     6661 tgtactaccc ggcgcacaac gccgggattt agttgccgtg gaaactttca gcccatctct
     6721 gcatgggctt ttttctccgt caattctctg atgcttcgcg ctttttatcc gtaaaaagct
     6781 ataatgcact aaaatggtgc aacctgttca ggagactgct ttatggcaac aggcacgcag
     6841 cccgatgctg ggcagatcct caactcgctg attaacagta ttttgttaat tgatgacaat
     6901 ctggcgatcc attacgccaa ccccgccgcg caacaactgc tcgcccaaag ctcccgcaaa
     6961 ttgtttggta cgccgttacc ggaactgttg agctacttct cattaaatat cgagctgatg
     7021 caagaaagtc tggaggcggg gcaaggcttt accgataacg aagtgacgct ggtcatcgat
     7081 ggtcgctcgc atatcctttc tgtgacggcc cagcgtatgc cggacggcat gatcctgctg
     7141 gagatggcac cgatggataa ccagcgccgc ttaagccagg aacaactaca acatgctcaa
     7201 caagtagctg cccgtgattt agtgcgcggc ctggcacatg agattaaaaa tccgcttggc
     7261 ggtttacgtg gcgcggcgca gttactcagc aaagcgttac ctgacccatc actactcgaa
     7321 tataccaaag tgattatcga acaggcggac cggctgcgaa atttggtcga ccgtctgttg
     7381 gggccgcagc tgcccggtac gcgcgttacc gaaagtattc acaaagtggc tgaacgcgtg
     7441 gtaacgctgg tgtcgatgga actgccggac aacgtgcggt tgattcgtga ttacgatccc
     7501 agcctgccgg aactggcgca cgacccggat caaattgaac aggtcttgct gaatattgtg
     7561 cgcaatgcgc tacaggcgct ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc
     7621 gcgtttcaac tgaccttaca cggcgagcgc taccggctgg cggcgcggat tgatgtggaa
     7681 gataacgggc cgggcattcc gcctcatttg caggatacgc tgttttaccc gatggtcagc
     7741 ggccgcgaag gtggcaccgg gcttggctta tccatcgccc gtaatttgat tgatcagcat
     7801 tcaggcaaaa ttgaatttac cagttggcca ggtcataccg agttctcggt ttacctgcct
     7861 atcaggaaat aaaggtgacg tttatgcaac gagggatagt ctgggtagtc gatgacgata
     7921 gttccatccg ttgggtgctt gaacgtgcgc tcgctggagc gggtttaacc tgtacgacat
     7981 ttgagaacgg cgcggaagta ctggaggcgc tggcgagcaa aacgccggat gtgctgcttt
     8041 cagatatccg tatgccggga atggacgggc tggcgctgct caagcagatt aaacagcgcc
     8101 atccaatgct tccggtcatc attatgaccg cacattccga tctggatgct gccgtcagcg
     8161 cctatcaaca aggggcgttt gattatctgc ccaaaccgtt tgatatcgac gaagcagtgg
     8221 cgctggttga gcgcgctatc agtcattacc aggaacagca gcagccgcgt aatattcagc
     8281 ttaacggccc aacgaccgat atcatcggcg aagcgcaagc catgcaggac gtgttccgta
     8341 ttatcggtcg gctttcgcgt tcttctatta gcgtgctgat taacggcgaa tccggcaccg
     8401 gtaaagaact ggtcgctcat gccctgcatc gccacagtcc gcgcaccaaa gcgccgttta
     8461 tcgcgctgaa tatggcagct atcccaaaag atttgatcga atcagaactg tttggccacg
     8521 agaaaggcgc gtttactggc gcgaatacca ttcgtcaggg gcgttttgaa caggccgatg
     8581 gcggcacgtt atttctcgac gaaattggtg atatgccgct ggatgtgcag acgcgtttgc
     8641 tgcgcgtgct ggcagacggt cagttttatc gcgttggcgg ctatgcgccg gtgaaagtgg
     8701 atgtgcggat tatcgctgcc actcaccaga atcttgaaca gcgggtgcag gaaggtaagt
     8761 tccgtgagga tctgttccac cgcctgaacg ttatccgcgt tcatctgccg ccgctgcgcg
     8821 aacgtcggga agatattccc cgtctggcgc gccatttttt acaggttgcc gcgcgcgaac
     8881 tgggcgtaga agcgaagttg ctgcatccgg aaaccgaagc tgctctgacg cgtctggcgt
     8941 ggccaggcaa cgtgcgccag ctggaaaaca cctgccgctg gctaacggtg atggccgccg
     9001 ggcaggaagt gttgattcag gatttgcccg gcgaactgtt tgaatcaacg gttgcggaga
     9061 gtacttcgca aatgcaaccg gacagttggg cgacactttt agcgcagtgg gcagacagag
     9121 cgctgcgttc cggtcatcaa aatctgcttt ccgaagcgca gccagagctg gagcggacgt
     9181 tactgacgac cgcgttgcga catacgcagg ggcataaaca ggaagcggcg cggctactcg
     9241 gctggggccg caacactctg acgcgtaagt taaaagagct ggggatggag tgattcacgg
     9301 cttgtgtgta aagattgatt attgagcgca aattgctggt attttgcgct ttactgttcc
     9361 gataagttca gtatgatctt gcccggaaaa cggggagagt cattatgctg gaatcaataa
     9421 ttaatctggt atcgagtggc gcagttgaca gccacacacc gcaaactgcc gttgctgccg
     9481 tgctgtgtgc cgcgatgatt gggctgttta gctgaagctc gttgtttatg ccggatgcgg
     9541 agaaagtcgt aggcctgata agacgcgacc agcgtcgcat caggcatcat gctcagatgc
     9601 ctgatgcggc gtaaacgcct tatccggcct acaaaagcaa taatttcaac aggttttgtt
     9661 gcccagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt
     9721 gtaaacgaag cgccattcac tctttaaatc acccgtgaga actgctgcat ccgcgctttc
     9781 tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc
     9841 gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc
     9901 agcttgagat cttcctcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaagaggg
     9961 gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca
    10021 tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatacac ttgctgatag
    10081 tactgcttca actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa
    10141 acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc
    10201 agcacgcctt cacgctgggc caccgccagc tcgtcatcag gacgggcaaa gtgatccata
    10261 ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt
    10321 ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc
    10381 ggcagatgcg cgtagttaaa aacgctcaga cgatcggggt tcagctccgc cacacgtttc
    10441 agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg
    10501 gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc
    10561 tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg
    10621 cgattaaagc cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg
    10681 acttcgatcg aaatctccgc atcggtattg aaatggaagt tttcgcgcag cagcttcatc
    10741 agacggctga tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg
    10801 ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc
    10861 agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcaacc gcagaagtaa
    10921 caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc
    10981 gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg
    11041 tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg
    11101 atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg
    11161 gcggatgccc gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc
    11221 gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa
    11281 cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt
    11341 gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca
    11401 actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggcg ctcagggttt
    11461 cgcctgcttc cagacgttcc agcagcgcat ccagacgctc atccgtttcc agtaattcca
    11521 actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt
    11581 tttcagtcac tcccaatgga ataggggttt tactgccaat acgtggatct tttggtgcgt
    11641 tctggccttt gctgcctgac gtggtgtttc cgcccgctgc gcggctgccc ggcgcatgac
    11701 cacgacgttt tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt
    11761 ttcgacgcgc ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg
    11821 ttcgtctttt agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc
    11881 aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg
    11941 gtcttacaat ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg
    12001 tgttgattcc atttccgttt aattacgtct ccggacgttt gtcttcctga caatcctctg
    12061 tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg
    12121 ctctatgggt gctactttac gcctttgctt taaacaaaca atcaaccgcc atcagtattt
    12181 gcacattttc agagttttat ataaaagcaa ttcactgatt tatatagctt atttgacatg
    12241 catcgcagaa ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcgt
    12301 atggctttta cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata
    12361 atcgccacaa agctttgact gacggagacg accgctttga ctaatttgaa ttatcaacag
    12421 acgcattttg tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa
    12481 gtggcttttg caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac
    12541 cagaaaagcc tggctcgtac ctcaaaaacc ccagggcgca cccagcttat caacctgttt
    12601 gaagtggctg acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg
    12661 gaagagatga agcgcaaatg gcagcgtgcg ctcggcgaat acctcgaaaa acgtcagagc
    12721 ctgcaaggtc tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcagcag
    12781 atgattgagt gggcggtaga cagcaatatc gccgttctgg tgctgctgac caaagcggac
    12841 aaactggcaa gcggcgcacg taaagcgcta ttgaatatgg tgcgtgaagc tgtactggcg
    12901 tttaacggtg atgtgcaggt tgaaacgttt tcttcgttga agaaacaagg cgtagacaag
    12961 ctgaggcaga aactggatac ctggtttagc gagatgcagc ctgtagaaga aacgcaggac
    13021 ggcgaataat tttcttgcct taatgcttgt gccggatgtg gcgtatccgg cccgtaaatt
    13081 caatagatta caattctacc gttggcctga taaacatagc gcatcaggca ttacggatct
    13141 tttctttcgc ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca
    13201 gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc
    13261 tacgcgacta actttactct tttttgttca gttcagaaag cactttttgt aatttttttt
    13321 cagttgttgc ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac
    13381 ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact
    13441 ccccacttcc accagcaacg gcacatccag acgggtacag ttttccatca gttgatgaat
    13501 ctgcttcgcg acggcatcaa catcatcttt atgaacttca aataccagtt catcgtgtac
    13561 ctgcatgatc atacgtacac gcggttgctc agcctgtaac cacgcatcaa cggcaatcat
    13621 cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc
    13681 agccgctgca cgacgagcac cattgctgga tttgatatcc ggcagataca gacggcgtcc
    13741 gtccagcgtt tcaacgtagc ccagctcttt cgcctgagca cgggtgcgtt ccatatactc
    13801 cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg
    13861 aatgttcaat tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat
    13921 cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc agtggcaaac caaacacttc
    13981 tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat gcggtcagca agcctttatc
    14041 acgcgagaga tgcgccataa tgcgcagttc aatctgcgag tagtccgctg agacaatcac
    14101 ataatcctct ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg
    14161 aatgttttgc aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg
    14221 ataagaggta tgcacacgcc cggttttcgg gttgatcatc agcggcagct tgtcggtgta
    14281 agtcgacttc agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc
    14341 cagcgccagt tcttccagta cctcttccga cgttgacggc gcgccacccg gcgttttctt
    14401 cagcggttta ataccctgtt tttcaaacag aatggtttgc aactgcttgg tggaagaaag
    14461 gttaaattct tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgcaaggt
    14521 gagctcttca gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat
    14581 gcgtgaaagc accggcacca gcggcatttc gatattctcg aagacgttca acggcccttt
    14641 gtgtttctgc agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc
    14701 ggcggcgtaa cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc
    14761 tttacccgca atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct
    14821 gtccatatcg tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa
    14881 cgcaatccca cgcagttcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt
    14941 ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg
    15001 ctcgtgagag atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc
    15061 tacgcctggc tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct
    15121 gtcggtttcg gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt
    15181 cagtgtttct tcatcaagga tagtgacgta gttgtcataa gaaatcaccg ttgccgtcac
    15241 ttctggcgct tcgtctgcaa cactggtttc ctgcggcttc gcggctgttt ttgccccttt
    15301 ggcctgtaac catttgcccg cttcgacatc agcagtccag cgtttgaact catacttttt
    15361 gaacagcccc aacaactctt ccgctgccgg ttgctgcact tccagttgtt cacaggtcag
    15421 ctccagttca acatcggttt taatcgtcgc caactggtat gagagataag caacttcttt
    15481 gttttgctcg agcttcgctg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt
    15541 ttctggctcg gcatacagcg tatccagccc gccaagacct tgcagcaatg cctgcgcggt
    15601 tttttcaccg acgcccggta cgccaggaat gttatcagag gagtcaccca tcagcgccag
    15661 gaagtcgatg atcaattctg gcggcacgcc gtacttattc accacctctt ccggtccgag
    15721 gatggtattc gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc
    15781 tttatcgcca gtgctaatca gcaccggacg cccggctttt tcggcttcgc gcgccagagt
    15841 accgataacg tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt
    15901 aaccatcgcg tgcagaggtt cgatttgtgc tcgcagatcg tccggcattg gcgggcgatg
    15961 tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac
    16021 cgctgcatgc gtcggtttat attgcatgat cagactgcgc agcatgttga ggacaccata
    16081 catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg
    16141 ataaagataa gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat
    16201 gtccgtgcct gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag
    16261 tttttcaccg cttttcgtga aaatcttttg tagatctgct ggatcgctcg caaatcgtca
    16321 tgtggataac tttgtgcata aattatttac atcgctttta atttattatc atgcttttta
    16381 cagcatgatg tttgtctttg tttttcatgc agttaaaaat aaatccatcc ctaataattg
    16441 agataatggg attttttcta atgtggatag atatgaatta tttttctcct taaggatcat
    16501 ccgttatttg ggtcattttc tgctaaaatc tgcgccttgc gcttctttag tgcactgtct
    16561 atggctaacc ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg
    16621 ctgagtatcg tattgaccat tttggtcact attttttgtt ccgtcccgat catcattgcc
    16681 gggattgtaa aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt
    16741 gattttatga tgtattgctg gtgtgaaggt ctggcggtat tactgcatct gaacccacac
    16801 ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt
    16861 tgtaaccacc gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt
    16921 ccgatgaata aatatttcct caagcaacag ctggcctggg tgccatttct tggcctggcg
    16981 tgctgggcgc tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat
    17041 ccggagcgac gtggtcaaga tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg
    17101 catcccacca ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag
    17161 caaacccact ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg
    17221 ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac
    17281 aataaccgtc agccgttctt cgatatgtta agcggtaaac tgacgcggat tgtcgtacat
    17341 gtggatttac agcctattgc cgatgagtta catggcgact acatcaatga caaaagcttt
    17401 aagcgccatt ttcagcaatg gctgaactca ttgtggcagg aaaaagaccg gcttctgacg
    17461 tctctcatgt catcacagcg tcagaataag taagttttac tcgcctctga atgagcagag
    17521 gcgagtgagt attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca
    17581 tctcctgtcc gttaaaaaca actttacccg gtgtataacg cagctgcagc gaggcggtat
    17641 tgttgtcaat cgtgattaac tgaaacatct gacctaatgt catcatcccg ctgatttgtt
    17701 tatcagcctg cttttgcgct ttttccgcat ccattccttc agataaatta agctgttttg
    17761 cggtttctgt gacgacatta agcggtaact tcacatcaaa attgagcgat ttgatatctt
    17821 tgtttgtgga tgatgaagat ttggctgggt cggcaatact gatatccaga ttggcattta
    17881 gttcgccgag tgcgttcttc catcttaccg gttgtttaat ggtcggctca cttttttgta
    17941 ataacggtaa atactctttg aacaatgcag cattcacttc ttgcagagca acttcatcgt
    18001 ttgctaactc agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgataa
    18061 attggcgcac tgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga
    18121 gttgctgatt cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat
    18181 ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt
    18241 cttccccgtg attgataatg ttaatctgat taaatttcga ctcgctttca ccaaccggaa
    18301 atttcttttc ttctaatcgc gccagagaat caatggtaaa actttttgcc gttactttgt
    18361 tcattggtga taattgcaga acaatatctt cgactttgcc ttcaatatcc aggttagaca
    18421 atgaactacc atcaccatta aaagtaaact ggccctcacc aaaagaaaat tttccatact
    18481 ctgcaggggc aacatctaat gtcgtagaga atgagccacc aaacgcgaat cgcaatgtcg
    18541 cttccacggg cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt tcattattca
    18601 ctaagttcac ttttgccgct gccagtgctg ggatgacatt accatgcatt aacattgtga
    18661 tgggcaacgg accgtgctca acatccacat caaatacaac tttctggcct ggcttgatat
    18721 tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg aaaacgggaa ttaaaaaagc
    18781 ctttgtcaaa gtttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttcg
    18841 catcgttaaa atctttaata aacttttcga taccaggctg aatttgcgta cctgtgtacc
    18901 atgtgccacc accccagatt acggctaacg caacaataac acctgtagct gacttacgta
    18961 tcatataaat attccctgat aaaacagcag aactttgaat atccacttat gctgaaataa
    19021 tgtccagcgg caggatgcat tatcagaatg tatttatatt tattaaataa taaaaaaagc
    19081 ccgtgaatat tcacgggctt tatgtaattt acattgaatt atttttcctc ggacagatat
    19141 ttcaccgtat cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc
    19201 ggattcagct gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacatca
    19261 gctgcggctt tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg
    19321 tcgtactctt cacctttaat acctgcattg ataaatacat cgcggatatc agatgctgaa
    19381 cgaatggtct gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg
    19441 cccagcgcca tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg
    19501 aagttgacgt ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca
    19561 gaaatatgca gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc
    19621 agcacttgcg gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca
    19681 tactgcgccg ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt
    19741 ttcatgatct actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag
    19801 aacttttgcc tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa
    19861 ccacgggaaa tttttcggga acgcgggatc agcccaatgc cgcattagcc aggcaagata
    19921 ataaaccaaa cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc
    19981 gctaaattct tcataagctt caataatagt ttccagttgc atccgctgct gggctttatc
    20041 gccattgagc aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc
    20101 aacaaacatt ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg
    20161 cagaacggtg aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc
    20221 tttcagaaat gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg
    20281 aggctcaatg agatattcat tcagaccgat ggtcgggcga tgaataaaaa gctgtttgcg
    20341 ccccgtctgg tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc
    20401 agcttcgaac tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt
    20461 caataaagtc tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccag
    20521 ctgcaacgca aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata
    20581 aaatttgacg acaaaacgtc gacgctcttc gtcctgaaat tgatagacac ggttttcata
    20641 gctgttaagc ggggtaagac cggaatccac ccggatccct tgctcaaaca gagcgtccat
    20701 gatggtatcc gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg
    20761 aaaacattac gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa
    20821 gcttttactc tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat
    20881 aatctttctg caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat
    20941 agatcagaat gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca
    21001 gtttctgcaa aaattgcaac aagttaaagt ctggctcttt ttgccaggct ggctggagga
    21061 gttcaataac ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag
    21121 acacgttagc agggtcaatc ccacaataaa agaggcgata tcggtgaatc tgatgacgac
    21181 gataacaggc gttgtgctgg caggcggtaa agccagacga atgggcggcg tagataaagg
    21241 attgctggac ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca
    21301 gctctctcac gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct
    21361 gaaagtgatt gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt
    21421 aatgcagcag gaagctggtg agtggttttt gttttgtccg tgcgatacgc cttacattcc
    21481 ccatgattta gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca
    21541 tgacggtgaa cgcgatcacc cgactattgc tctggtaaac cacgctattg agcctttatt
    21601 actggaatat ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg
    21661 tcatgcggtt gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga
    21721 gcttgcccga tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt
    21781 accggaaaaa ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt
    21841 ccggggctga ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc
    21901 tatgagctgc gcaaggctgg cgcggcacaa accatcgttg ccagccagca acgatgggcc
    21961 ttgatgacgg aaacaccaga cgaagaagag ctggatctac attttctcgc aagtcgaatg
    22021 gatacctcaa agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag
    22081 attgtgctgt ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat
    22141 gttattgctg tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat
    22201 gatgttgagg ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca
    22261 agagtaggct ggatcagacg cagccagcgt cgcacccggc atttttttca ccaacgcaaa
    22321 aaggccatcc tgacggatag ccttt
//
LOCUS       DAOFMX010000066        21500 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.066, whole genome shotgun sequence.
ACCESSION   DAOFMX010000066 DAOFMX010000000
VERSION     DAOFMX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.066"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(38..2107)
                     /gene="glyS"
                     /locus_tag="QBA20_002893"
     CDS             complement(38..2107)
                     /gene="glyS"
                     /locus_tag="QBA20_002893"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDQ6293354.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGIIV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2117..3028)
                     /gene="glyQ"
                     /locus_tag="QBA20_002894"
     CDS             complement(2117..3028)
                     /gene="glyQ"
                     /locus_tag="QBA20_002894"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDQ6293355.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3123..3422)
                     /locus_tag="QBA20_002895"
     CDS             complement(3123..3422)
                     /locus_tag="QBA20_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDQ6293356.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3597..4592
                     /gene="wecH"
                     /locus_tag="QBA20_002896"
     CDS             3597..4592
                     /gene="wecH"
                     /locus_tag="QBA20_002896"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDQ6293357.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4634..5071)
                     /locus_tag="QBA20_002897"
     CDS             complement(4634..5071)
                     /locus_tag="QBA20_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDQ6293358.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5117..5458)
                     /locus_tag="QBA20_002898"
     CDS             complement(5117..5458)
                     /locus_tag="QBA20_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293359.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5627..7081)
                     /gene="xylB"
                     /locus_tag="QBA20_002899"
     CDS             complement(5627..7081)
                     /gene="xylB"
                     /locus_tag="QBA20_002899"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDQ6293360.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGSARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7153..8475)
                     /gene="xylA"
                     /locus_tag="QBA20_002900"
                     /pseudo
     CDS             complement(7153..8475)
                     /gene="xylA"
                     /locus_tag="QBA20_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8841..9833
                     /gene="xylF"
                     /locus_tag="QBA20_002901"
                     /pseudo
     CDS             8841..9833
                     /gene="xylF"
                     /locus_tag="QBA20_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
     gene            9911..11452
                     /gene="xylG"
                     /locus_tag="QBA20_002902"
     CDS             9911..11452
                     /gene="xylG"
                     /locus_tag="QBA20_002902"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDQ6293361.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11430..12611
                     /gene="xylH"
                     /locus_tag="QBA20_002903"
     CDS             11430..12611
                     /gene="xylH"
                     /locus_tag="QBA20_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDQ6293362.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSTRNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12689..13219
                     /locus_tag="QBA20_002904"
     CDS             12689..13219
                     /locus_tag="QBA20_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDQ6293363.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13331..13867
                     /locus_tag="QBA20_002905"
     CDS             13331..13867
                     /locus_tag="QBA20_002905"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDQ6293364.1"
                     /translation="MIGIDNEELTRYLSRIALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPDVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13975..14799)
                     /locus_tag="QBA20_002906"
     CDS             complement(13975..14799)
                     /locus_tag="QBA20_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDQ6293365.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15119..17149
                     /gene="malS"
                     /locus_tag="QBA20_002907"
                     /pseudo
     CDS             15119..17149
                     /gene="malS"
                     /locus_tag="QBA20_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17327..18580
                     /gene="avtA"
                     /locus_tag="QBA20_002908"
     CDS             17327..18580
                     /gene="avtA"
                     /locus_tag="QBA20_002908"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDQ6293366.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18731..19204)
                     /locus_tag="QBA20_002909"
     CDS             complement(18731..19204)
                     /locus_tag="QBA20_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDQ6293367.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19306..20154)
                     /gene="yiaJ"
                     /locus_tag="QBA20_002910"
     CDS             complement(19306..20154)
                     /gene="yiaJ"
                     /locus_tag="QBA20_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDQ6293368.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20355..21347
                     /gene="yiaK"
                     /locus_tag="QBA20_002911"
     CDS             20355..21347
                     /gene="yiaK"
                     /locus_tag="QBA20_002911"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDQ6293369.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRD
                     AKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21359..>21500
                     /locus_tag="QBA20_002912"
     CDS             21359..>21500
                     /locus_tag="QBA20_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDQ6293370.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5406 a         5489 c         5275 g         5330 t
ORIGIN      
        1 agcctgccag atggcaggct ttttaataac ggcgttatta ctgcaacagc gaaatatccg
       61 caacgcgcag gaacagttcg cgcagtttct ccagcatggt cagacggttg agacgcaatt
      121 ctttgtcatc aaccatgacc atcactttat cgaagaaagc atcaaccggc tcacgcagct
      181 ctgccagttc gaccagcgca tcctggtaac gaccttccgc aaagtacggc tccagcttgt
      241 cacgtagcac cacaacctgc atcgccagtt taatttcttc cggctctttc agggtagagg
      301 cattcacgcg atcgctcagc acttcgtcag atttcgccag gatgttagat acacgtttgt
      361 tcgccgccgc cagtgctgca gctgcttcca gggtacggaa gtgcgatacc gctttcatgc
      421 gggcatcgaa atcagccgga cgagtcggac gacgcgccag taccgcctgg atggtgtcaa
      481 cggtataacc ttcgtcctga taccaggcgc ggaagcgacc gagcataaag tcgataacat
      541 catcaactac gttggcatta gtcagcttat cgccatacag acgcaccgct tcttcggtca
      601 gcgtttgcag atcaaggttg aggttcttct caacgataat tcgcagcacg ccaagtgcgg
      661 cacgacgcag cgcaaacggg tctttgtcgc ctttcggatg ctgaccgata ccgaagatac
      721 ccgccagagt atccatctta tcagcaatcg ccagcgcaca agctaccggg ttggacggca
      781 ggtcatcacc ggcaaagcgc ggctgatact gctcattcag cgcgacggct acgtcttccg
      841 cttcgccatc gtgacgcgcg tagtgcatcc ccataacgcc ttgggtgtcg gtgaactcga
      901 agaccatgtt ggtcatcagg tcacacttag acagcaggcc cgcacgggtt gcgtggttaa
      961 cgtcagcgcc aatctgttcg gcgatccagc cagccagcgc ctggatgcgg tcagtcttgt
     1021 cacgcagcgt acccagctgt tgctggaaca gcacggtttg caggcgcggc aggttatctt
     1081 ccagacgttt tttgcggtcg gtgttgaaga agaactcggc atccgccaga cgtggacgaa
     1141 cgactttctc gttaccagag ataatttgct gcggatcttt cgattcgatg ttggcaacga
     1201 agataaagtt cggcagcagt ttgccgtcgt tcgcatacac cgggaagtac ttctggtcac
     1261 ctttcatggt gtaaaccagg gcttcagccg gcaccgcgag gaatttctct tcgaattttg
     1321 cggtcagaac gaccggccac tccaccagcg aagccacttc ttccagcagg ctttcgctta
     1381 agtcagcgtt accgccaatc ttacgcgctg cttcttcggc atcggcttta atcttcgctt
     1441 tacgttcttc gtaatcggcg atgactttcc cgcgctcacg cagaatttcc ggatactgat
     1501 cggcgttgtc gatggtgaat tccggttcgc ccataaagcg gtggccgcga atcacgcgat
     1561 cggactgaat gcccagaatg gttgccggaa tgactttgtc gcccagcagc agggtcacgg
     1621 tgtgcaccgg acgcacgaag tgcacgtcgc ttgcacccca gcgcatcagt ttcgggatcg
     1681 gcagtttcgc cagcgaagtc gcaaccatat tcggcagcag tgcttcggtg ctttcgccct
     1741 tcacatgggc gcgatacagc agccattcgc ctttatcggt agtcagacgc tcagcctggt
     1801 caacgataat accacaacca cgcgcccaac cttctgccgc tttgctcggt tttccttcag
     1861 cgtcgaacgc ctgggcaatc gccgggccgc gtttttcgat ttcacgatct ggttgtgctt
     1921 ccgccaggtt agctactttc agcgccagac gacgcggagc agcaaaccat tgaacggtgc
     1981 cgtgtgcgag gcctgcgtta tccagctccg cagtaaagtt cgcagcaaag gactctgcca
     2041 ggctgcgcag tgcttttggt ggcagctctt cagtgccgat ttccaccaga aaagttttct
     2101 cagacatagc cgcctcttac ttatctttgt tgcacatcgg gaagccgagg gcttcacggg
     2161 aagcgtaata tgcttctgcc actgctttgg tcagggtgcg aatgcgcaga atgtagcgct
     2221 gacgctcggt gacggagatg gctttacgcg catccagcag gttgaagctg tgggcggctt
     2281 tcagaatacg ctcgtaggct ggcagcggca gcggattttc cagcgccagc agctgctgcg
     2341 cttctttctc gtactgctca aagcaggtga acaggaagtc cacatccgcg tattcgaagt
     2401 tgtaagtgga ctgctccact tcgttctgat ggaacacgtc gccgtaggtg gttttaccca
     2461 gcgggccgtc gctccagact aggtcgtaaa cgctgtctac gccctgaatg tacatggcca
     2521 gacgttccag accgtaggtg atctcgccgg taaccggttt acactccaga ccaccaacct
     2581 gctggaagta agtgaactgc gtcacttcca tgccgtttag ccacacttcc cagcccagtc
     2641 cccaggcccc cagagtcggg ttttcccagt tatcttccac gaaacggatg tcgtgaatag
     2701 tcgggtccat gcccagctct ttcagagaac cgaggtacag ctcctgaata ttgtccggcg
     2761 atggcttaat gaccacctgg aactgatagt agtgctgtaa acggttgggg ttttcgccgt
     2821 agcgaccatc ggtcgggcga cgagaaggct gaacataagc agccgccatc ggttctggcc
     2881 ccagcgcgcg cagacaggtc attgggtgag aggttcccgc gccgacttcc atgtccaatg
     2941 gttgaacaat ggtgcagccc tggcgagccc agtaatcctg taaggtcagg atcaagccct
     3001 ggaaggtcct ggtatcaaac ttttgcatat tatttcgtgc tggatacgtg tggatttaaa
     3061 ggaagggatc agtatacccg ctggatggaa gatatacagt acgaaacggg aaaaagcagg
     3121 gcttaacgca tggaaagatg caaaaaatgg ccatctgcgt caaaagaaca gacaaactgc
     3181 tctttacgga aggtataacc gcgcatttca tagcttccct ggaactgttc gaaggcggtg
     3241 acgtcgattt tttgctcgcc ggtgttataa cgttttgccg cctgatcctt gcataacgct
     3301 tccatattca acgaacgcag aggatcaacc tttacccgtt gtgctttctg cacgggtgac
     3361 ggtgtagaac aacccactag caccataagc gccattgccg gaaagaaagc gttcatcatc
     3421 attaaattac cgtctggttt gcaggtgagt cttattattt atatcggtat agtggctgat
     3481 atcttacgaa tttccttaag cctctatagg ctgtagtgat aaaactcaga ttattccagt
     3541 gcgccaacca aatgatttat cggtcagact ttttttaacc agatacagag acacagatgc
     3601 agcccaaaat ttactggatt gataacctgc gagggatagc gtgtttaatg gtggtgatga
     3661 ttcacaccac tacctggtat gtgaccaatg ctcatagtgt tagccccgtc acatgggata
     3721 tcgccaatgt tctgaattct gcctctcgtg tcagcgtgcc gctatttttc atgatttccg
     3781 gctatctctt ttttggcgaa cgcagcgccc agccgcgcca tttcttgcgt atcggcttat
     3841 gtctgatttt ttatagcgca atcgcactgc tctacattgc gctgtttacc tccatcaata
     3901 tggagttagc gctgaaaaac ctgctgcaaa agccagtgtt ttaccacttg tggtttttct
     3961 tcgcgattgc ggtgatttat ctggtttcac cgctgattca ggtgaagaac gtcggcggaa
     4021 aaatgttgct ggtgctaatg gtggtgattg gcattatcgc taacccaaac acagtgccgc
     4081 agaaaattga cggttttgaa tggctgccaa ttaacttata tatcaatggc gatacttttt
     4141 actacattct gtatggcatg ttgggccgcg ctatagggat gatggacaca cagcataaag
     4201 cactgtcgtg ggtgagcgcc gcgctgtttg cgacgggggt ttttattatc tctcgcggga
     4261 cattatatga attgcagtgg cgcggaaatt ttgccgatac ctggtatctt tactgtgggc
     4321 cgatggtttt tatctgcgca atcgcgctat tgactctggt taaaaacacg ctggatacgc
     4381 gtaccattcg cggacttggc ttaatctccc gccattcatt gggtatatac ggattccacg
     4441 ccttgattat ccatgcgctg cgcacccggg gaattgagct taaaaattgg ccaatactgg
     4501 atattatttg gatcttttgc gcgacgttgg cagcgagttt gttactttct atgctggtac
     4561 aacgaatcga cagaaacaga ttagtgagtt aagtaaaagc ccgttcacat tggactgacc
     4621 gggcttacgt gaattattcg ctgtattctt cctgggtaac ctgtgtttct tttggcgggt
     4681 ttattccggc atcacgaata ttcttctgca ccgctactgc accaaacaag cttaagaaga
     4741 aagccagtcc ataaaaacct ttttcactga gtaataatgt cgcattccac agacctacca
     4801 tcagtagagc aacagagata ataaagacag tcaggcaggt catataataa atggaagtgg
     4861 tcggtatgcc ttcatacttg tcccgaacgg tcttttgata agacgcagca gaaaacagtc
     4921 ctaacaccag tacggcaaaa taataccctt tttcatttaa ctgcatctct gcattccata
     4981 gccctaacag gtaggtaacg ataccaccaa cgagagctat ccatgacaca atactaaagg
     5041 ccggtgaata ggttgatatt ttgttgtcca tcacagtatt cctttcattc ctgaatgtgt
     5101 aagagcttta tgttgctcat accgagcgaa acaaacgcat ttgaccaaac aagcagacaa
     5161 aaaaggcgat ggggtagatc accatttcat aagcattgat aggtgcattt aaaacaccga
     5221 taaacaagag accgatagtg attaacgcaa ctaactggca gatatgggta aaaaaattat
     5281 ccagttgccc gagtttttct gcgcgaagat atgcatagtt accaaaagtt gctgtcatta
     5341 acacgccaag aaaatagcct tttccactta gcaatgggca tgagatccat agcccaacca
     5401 gataaaccag cgccccgaca acaaataata gttttgccac agtttttgag gttttcataa
     5461 cgatctccat atctaccagc gatacattac gagtaaccaa cgaaagacaa aactgaaaaa
     5521 tgccattaac aaatgatttt cagaataaat tcatactaaa tattaattaa ttagtgagat
     5581 atatagatgt gaattatccc ccacccggtc aggcagggga taacgtttac gccattaatg
     5641 gcagaagttg ctgatagagg cgacggaacg tttcacgtcg tggctgataa gcggcataac
     5701 gctgcgcatc tggtagatgc gactgttcta acggtagctg cggcaacaac tcaatgagcg
     5761 atttctctgg attcgccgca atctgcgcca gccttgctga gcccagtgct ggccccacat
     5821 ccccccccgt acggtaatcg agctgctgac cgctgatatc cgccagcatc tgacgccagt
     5881 actcactacg cgccccgcca ccaatcaacg taacactttg cggtttaatg ccgcaggcat
     5941 gcacgacatc catgccatct gccagcgcat aacccacgcc ttccagcact gctcgcgcca
     6001 gttcattggg gccatgttga tgagtcaaac caaagaaaac ccccttcgcc tggggattat
     6061 tgtgtggtgt acgctcgccg gaaagataag gcagaaacca aactggctcg gcactttcat
     6121 cagcctgttg agctgcagcg attaaagctg ggacattgca caggccggtt aatttcgcgg
     6181 cccaatccag acacgatgct gcactcagca ttacagacat taaatgccaa cgctgcggta
     6241 gcgcatggca aaagctgtgt acagcgcttt ctggcttgct taagaaccct tcgctgacag
     6301 caaaatagac ccccgacgtc cccagcgata acattgcctg attagcatca accattccca
     6361 caccaactgc accagctgca ttgtcgccac cgcctgcgac aactggcacc gtcgccatac
     6421 cccacgcttt cgcaacttca ggtaacaaag caccagtaat ttcgctgcct tcgtataatg
     6481 cgggcatctg gtcacgagat aagtcgcaag cctgcagcat gacgtcactc cagtcacgct
     6541 ttgcgacatc cagccacatg gtgccagctg cgtcagacat atcgctggca aactcccccg
     6601 tcatacgcag acgcaagtaa tctttcggta ataatacttt gtcgatttga cggaatatct
     6661 ccggctcatg ccgctgaacc catagcaatt taggcgcagt aaatccgggc atcatcaggt
     6721 tgccggtaat cactcgtgat tgcggaactc tcgcttccag caaagtgcac tcttgcgcac
     6781 agcgcccgtc gttccacaaa atggcagggc gcaatacccg ttgttgagca tccagtaagg
     6841 ttgctccatg catctggccg gcaataccca atgctttaac gtcctgcaga gaatgctgat
     6901 cgcccagagc tttcattgcg cgatcagttg cctgccacca ctgttccggg tcttgttccg
     6961 accaaagtgg atgcgggcgc gaaacggtca gcttttccgt ttgcgaagca accacctcac
     7021 cctgctcgtt gagcaaaata acttttacgc ccgaggtgcc aagatctatc ccgatataca
     7081 tatcgatcgt tccttaaaaa aatgcccggt atcgctaccg ataaccgggc caacggactg
     7141 cacagttagc cgttatttgt cgaacagata atgatttacc agattttcca gttgctcctg
     7201 gcgaccactc tgatgcaccg gagacaaatt atgttcctga gcatatttgg ctaaatctgc
     7261 cagtgacatt tggcctttca ggatttgctg gcccaattcg ctattccagc cggaataacg
     7321 ctgcgcgatg cgtttatcca gctcgccatc ttcaatcatg cgcgctgcaa ttttcagcgc
     7381 cagtgccatc gtatccatcg cgccgatatg accgtaaaac agatcatatt tatcagtact
     7441 ttgacgacgt actttggcat cgaagttcag accaccggtg gtgaaaccgc ctgctttgag
     7501 aatttcatac atcaccagcg cattctcttc cacactgttc gggaactggt cggtgtccca
     7561 gcccagttgc gcatcgccac ggttggcgtc gacagaaccg aacaggccaa gcgcaatggc
     7621 ggtggctatt tcatgatgga aagagtgacc tgccagcgtc gcgtggttag cttcaatgtt
     7681 cagtttaatc tctttttcca gaccaaactg tttcaggaag ccatagaccg tcgcggcatc
     7741 gtaatcatat tgatgtttag tcggttcttg cggtttcggt tcgataagca acgtgcccta
     7801 gaagccgatt ttatgtttat gctcaaccac catctgcata aagcggccca gttgttcacg
     7861 ctcctgacgc aagtcggtat ttaacagcgt ttcgtaacct tcacgaccac cccacaggac
     7921 atagttttca ccgcccaatt tatgggttgc ttccatcgct gtaacaactt gcgttgccgc
     7981 ccagctgaag acttcaggat ctgggttcgt cgccgcaccc gcgccatagc gagggtttgt
     8041 aaagcagtta gcggttcccc acagcagctt cacgccgctc tcttcttgct tggctgccag
     8101 gacatcaacc atttgcgcaa aattattgat gtactctttt aacgacgcgc cctcagggga
     8161 aacatccaca tcgtggaagc aataaaatgg cacatgtaac ttgtggaaaa actcaaatgc
     8221 gacatctgct ttacgcttcg ccaacgccag tgcctcacca ggctgctgcc acggacgatt
     8281 aaacgccccc acaccaaaca tgtccgcccc gttccagcag aaggtgtgcc agtagcaggc
     8341 ggcaaaacgc aagtgctctt ccatacgctt acccaacacc agttcgtcgg gattgtagtg
     8401 acggaatgct aacgggtttg aggattttga gccttcataa cgaacgcgat cgagctggtc
     8461 aaaataggct tgcatattga actccataat caggtaatgc cgcgggtgat ggatgatgtc
     8521 gtaatattgg gcactccctt tcaattgctc aattatgtta tttcacactg ctattgagat
     8581 aattcacaag tgtgcgctcg ctcgcaaaat aaaatgggat gatgaaactg ggtaattcct
     8641 cgaagataaa aatgcaataa gtacaattgc gcaacaaaag taagatctcg gtcataaatc
     8701 aagaaataaa ccaaaaatcg taatcgaaag ataaaaatct gtaattgttt tcccctgttt
     8761 agttgctaaa aattggttac gtttatcgcg gtgattgtta cttattaaag ctgtcctcta
     8821 actacagaag gccctacacc atgaaaataa agaacattct actcaccctt tgcacctcac
     8881 ttctgcttac caacgttgcg gcacacgcca aagaagtcaa aataggtatg gcgattgatg
     8941 atctccgtct tgaacgctgg caaaaagatc gagatatttt tgtgaaaaag gcagaatctc
     9001 tcggtgcgaa agtatttgta cagtctgcaa atggcaatga agaaacacaa atgtcgcaga
     9061 ttgaaaacat gattaaccgg ggtgtcgatg ttcttgtcat tattccgtat aacggtcagg
     9121 tattaagtaa cgttgtaaaa aaagccaaac aagaaggtat taaagtatta gcttacgacc
     9181 gtatgattaa cgatgcggat atcgattttt atatttcttt cgataacgaa aaagtcggtg
     9241 aactgcaggc aaaagccttg gtcgatattg ttccgcaagg taattacttc ctgatgggcg
     9301 gctcgccggt agataacaac gccaagctgt tccgcgccgg acaaatgaaa gtgttaaaac
     9361 cttacgttga ttctggaaaa attaaagtcg ttggtgacca atgagttgat ggctggttac
     9421 cggaaaacgc attgaaaatt atggaaaacg cgctaaccgc caataataac aaaattgatg
     9481 ctgtagttgc ctcaaacgat gccaccgcag gtggggcaat tcaggcatta agcgcgcaag
     9541 gtttatcagg gaaagtagca atttccggcc aggatgcgga tctcgcaggt attaaacgta
     9601 ttgctgccgg tacgcaaact atgacggtgt ataaacctat tacattgctg gcaaatactg
     9661 ccgcagaaat tgccgttgaa ttgggcaatg gtcaggagcc aaaagcagat acctcactga
     9721 ataatggcct gaaagatgtc ccctcccgcc tcctgacacc gatcgatgtg aataaaaaca
     9781 acatcaaaga tacggtaatt aaagacggat tccacaaaga gagcgagctg taagcgttac
     9841 gccccggcgc ggagcggggg cgtgatttct ctccatgccg cgtgaatgaa ttggcttagg
     9901 tggagtcgtt atgccttatc tacttgaaat gaagaacatt accaaaacct tcggcagtgt
     9961 gaaggcgatt gataacgtca gcttgcggtt gaatgctggc gaaatcgtct cactctgtgg
    10021 ggaaaatggg tctggtaaat caacgctgat gaaagtgctg tgtggtattt atccccatgg
    10081 ctcctacgaa ggcgaaatta tttttgcggg agaagagatt caggcgagtc acatccgcga
    10141 taccgaacgc aaaggtatcg ccatcattca ccaggaattg gccctggtga aagaattgac
    10201 cgtgctggaa aatatcttcc tgggtaacga aataacccac aatggcatta tggattatga
    10261 cctgatgacg ctacgctatc agaagctgct cgcacaggtc agtttatcca tttcacctga
    10321 tacccgcgtt ggcgatttag ggcttgggca acaacaactg gttgaaattg ccaaggcact
    10381 taataaacag gtgcgcttgt taattctcga tgaaccgaca gcctcattaa ctgagcagga
    10441 aacgtcggtt ttactggata ttattcgcga tctacaacag cacggtatcg cctgtattta
    10501 tatttcgcac aaactcaacg aagtcaaagc gatttccgat acgatttgcg ttattcgcga
    10561 cggacagcac attggtacgc gtgatgctgc cggaatgagt gaagacgata ttatcaccat
    10621 gatggtcggg cgagagttaa ccgcgattta ccctaatgaa ccacatacca ccggagatga
    10681 aatattacgt attgaacatc tgacggcatg gcatccggtc aatcgtcata ttaaacgagt
    10741 taatgatgtc tcgttttccc tgaaacgtgg cgaaatactg ggtattgccg gactcgttgg
    10801 tgccggacgt accgagacca ttcagtgcct gtttggcgtg tggcccggac aatgggaagg
    10861 aaaaatttat attgatggca aacaggtaga tattcgtaac tgtcagcaag ccatcgccca
    10921 ggggattgcg atggtacccg aagacagaaa gcgcgacggc atcgttccgg taatggcggt
    10981 tggtaaaaat attaccctcg ccgcactcaa taaatttacc ggtggcatta gccagcttga
    11041 tgacgcggca gagcaaaaat gtattctgga atcaattcag caactcaaag ttaaaacgtc
    11101 gtcccccgac cttgctattg gacgtttgag cggcggcaat cagcaaaaag cgatcctcgc
    11161 ccgctgtctg ttacttaacc cgcgcatcct cattcttgat gaacctacca ggggtattga
    11221 tattggcgcg aaatatgaga tctacaaatt aattaaccaa ctcgtccagc agggtattgc
    11281 cgttattgtc atctcttccg aattacctga agtgctcggc cttagcgatc gtgtactggt
    11341 gatgcatgaa gggaaactaa aagccaacct gataaatcat aacctgactc aggagcaggt
    11401 gatggaagcc gcattgagga gcgaacatca tgtcgaaaag caatccgtct gaagtgaaat
    11461 tggccgtacc gacatccggt ggcttttctg ggctgaaatc actgaatttg caggtcttcg
    11521 tgatgattgc agctatcatc gcaatcatgc tgttctttac ctggaccacc gatggtgcct
    11581 acttaagcac ccgtaacgtc tccaacctgt tacgccagac cgcgattacc ggcatcctcg
    11641 cggtaggaat ggtgttcgtc ataatttctg ctgaaatcga cctttccgtc ggctcaatga
    11701 tggggctatt aggtggcgtc gcggcgattt gtgacgtctg gttaggctgg cctttgccac
    11761 ttaccatcat tgtgacgttg gttctgggac tgcttctcgg tgcctggaac ggatggtggg
    11821 tcgcgtaccg caaagtccct tcatttattg tcaccctcgc gggcatgttg gcatttcgcg
    11881 gcatactcat tggcatcacc aacggcacga ctgtatcccc caccagcgcc gcgatgtcac
    11941 aaattgggca aagctatctc cccgccagta ccggcttcat cattggcgcg cttggcttaa
    12001 tggcttttgt tggttggcaa tggcgcggaa gaatgcgccg tcaggctttg ggtttacagt
    12061 ctccggcctc taccgcagta gtcggtcgcc aagctttaac cgctatcatc gtattaggcg
    12121 caatctggct gttgaatgat taccgtggcg ttcccactcc tgttctgctg ctgacgttgc
    12181 tgttactcgg cggaatgttt atggcaacgc ggacggcatt tggacgacgc atttatgcca
    12241 tcggcggcaa tctggaagca gcacgtctct ccgggattaa cgttgaacgc accaaacttg
    12301 ccgtgttcgc gattaacgga ttaatggtag ccatcgccgg attaatcctt agttctcgac
    12361 ttggcgctgg ttcaccttct gcgggaaata tcgccgaact ggacgcaatt gcagcatgcg
    12421 tgattggcgg caccagcctg gctggcggtg tgggaagcgt tgccggagca gtaatggggg
    12481 catttatcat ggcttcactg gataacggca tgagtatgat ggatgtaccg accttctggc
    12541 agtatatcgt taaaggtgcg attctgttgc tggcagtatg gatggactcc gcaaccaaac
    12601 gccgttcttg attttgataa aaattttctc aaagccggtt acgtattacc ggttttgagt
    12661 ttttgcatga ttcagcagga aaagaaccat gtttactaaa cgtcaccgca tcacattact
    12721 gttcaatgcc aataaagcct atgaccggca ggtagtagaa ggcgtagggg aatatttaca
    12781 ggcatcacaa tcagaatggg atattttcat tgaagaagat ttccgcgccc gcattgataa
    12841 aatcaaggac tggttaggag atgtcgtcat tgccgacttc gacgacaaac agatcgagca
    12901 agcgctggct gatgtcgacg tccccattgt tggggttggc ggctcgtatc accttgcaga
    12961 aagttaccca cccgttcatt acattgccac cgataactat gcgctggttg aaagcgcatt
    13021 tttgcattta aaagagaaag gcgttaaccg ctttgctttt tatggtcttc cggaatcaag
    13081 cggcaaacgt tgggccactg aacgcgaata tgcatttcgt cagcttgtcg ccgaagaaaa
    13141 gtatcgcgga gtggtttatc aggggttaga aaccgcgcca gagaactggc aacacgcgca
    13201 aaatcggctg gcagactgac tacaaacgct gccaccgcaa accgggatta ttgccgttac
    13261 tgacgcccga gcgcggcata ttctgcaagt atgtgaacat ctacatattc ccgtaccgga
    13321 aaaattatgc gtgattggca tcgataacga agaactgacc cgctatctgt cgcgtatcgc
    13381 cctttcttcg gtcgctcagg gcgcgcggca aatgggctat caggcggcaa aactgttgca
    13441 tcgattatta gataaagaag aaatgccgct acagcgaatt ttggtcccac cagttcgcgt
    13501 cattgaacgg cgctcaacag attatcgctc gctgaccgat cccgacgtta ttcaggccat
    13561 gcattacatt cgtaatcacg cctgtaaagg gattaaagtg gatcaggtac tggatgcggt
    13621 cgggatctcg cgctccaatc ttgagaagcg ttttaaagaa gaggtgggtg aaaccatcca
    13681 tgcaatgatt catgccgaga agctggagaa agcgcgcagt ctgctgattt caaccacctt
    13741 gtcgatcaat gagatatcgc aaatgtgcgg ttatccatcg ctgcaatatt tctactctgt
    13801 ttttaaaaaa gcatatgaca caacgccaaa agagtatcgc gatgtaaata gcgaggtcat
    13861 gttgtagcga tgatgagaat tgtcggcgtc acatcaggga actcagcaca gccacctgat
    13921 gcgagcaaaa gccttatccg accgacaaat cagataaggc tcgaggaaat gaaattacat
    13981 atgcgccgcg attaaccgtt ggttatcctg gtacattgcg aacaggtagt tgttataact
    14041 cttccctttg gtcgagtagc ccttcagctt gtgaatcatc gctgtggcag tcacttcctg
    14101 atccgcttta cgcagctgtg cacgcgattt acggaacgaa gagtaagccg ggtgcgtatt
    14161 caggttagtg acataggcgc tcaccgattc tttgacagaa ctaaactgtg agtacccttt
    14221 cactttaccc ggcgcattgg tacaacgtcc tttcatgcat ttcatgccga acagattgtt
    14281 attgttgcgc gccagcttcg acgttcccca accgctttct gctgcagcca tcgtcgccac
    14341 catactggta gggataatgt ctacgcgttc aagcaaggta ttccacggga tttttcgcgt
    14401 attaccagac cacttcacct tgtagcgttt ggcgatgtct ttcagacgcg cacgctcagc
    14461 aggtgaccat tggccctgat actgttttga aatcagccag ttacgttctg cagtaatggc
    14521 tgcattttgg ctggtaatgt aaggcattac ggtccggaga aacgcctttt tccttggtgt
    14581 tccggaaggg tattttcgca aatcaggaag tgaactactc tttgcactat tgcgagaata
    14641 ctcttgttta ctgcttacct gtttattact agctttagtt aagtgggact tttgactcgc
    14701 tgttgttgtg tgcgtcttcg ctaacacctc actcgaaaac accagagtga gtaacataag
    14761 aatcatcgcc ccatatcgtc gtatgggagt caaaatcatc aggtctcctg gtcggattta
    14821 atcattccaa caccttatat ttttcacaaa tttgagagtt gaatctcaaa tcatatcaaa
    14881 aatagctgtc aagagcaccc caaggaatag tccaaatctg aaactatgtc acgagttaac
    14941 gattcagatt ggcgctaaat cgcagaaaat gtgggggtta tcgcaaaatt cagccgtttt
    15001 ttgcgcgcga tcgctcaccc ttgcttctca tcctgtggac ttaccgctca gggatgagtt
    15061 ttgtctggct tatcgctggc aaactgtctg aaatcgcagc aataaggact catccaccat
    15121 gaaactcgcc gcctgttttc tgacactcct tcctggcttc gccgttgccg ccagctggac
    15181 ttctccgggg ttccctgcct ttagcgaaca gggaacggga acatttgtca gccacgcgca
    15241 gttgcccaaa ggtacgcgtc cactcacgct aaattttgac caacagtgct ggcagcctgc
    15301 agatgcgata aaactcaatc agatgctttc cctgcaacct tgtagcaaca cgccgcctca
    15361 atggcgattg ttcagggacg gcaaatatac gctgcaaata gacacccgct ccggtacgcc
    15421 aacattgatg atttccatcc agaacgccgc cgaaccggta gcaaacctgg tccgtgaatg
    15481 cccgaaatgg gatggattac cgctcacgct ggatgtcagc gccactttcc cggaaggagc
    15541 cgccgtacgg gattattaca gccaacaaat tgcgatagtg aagaacggtc aaataacgtt
    15601 acaacccgct gctaccagca acggtttact cctgctggaa cgggcagaaa ctgacacatc
    15661 cgcccctttc gactggcata acgccacggt ttactttgtg ctgacagatc gtttcgaaaa
    15721 cggcgatccc agtaatgacc agagttacgg acgtcataaa gacggtatgg cggaaattgg
    15781 cacttttcac ggcggcgatt tacgcggcct gaccaacaaa ctggattacc tccagcagtt
    15841 gggcgttaat gctttatgga taagcgcccc atttgagcaa attcacggct gggtcggcgg
    15901 cggtacaaaa ggcgatttcc cgcattatgc ctaccacggt tattacacac aggactggac
    15961 gaatcttgat gccaatatgg gcaacgaagc cgatctacgg acgctggttg atagcgcaca
    16021 tcagcgcggt attcgtattc tctttgatgt cgtgatgaac cacaccagct atgccacgct
    16081 ggcggatatg caggagtatc agtttggcgc gttatatctt tctggtgacg aagtgaaaaa
    16141 aacgctgggt gaacactgga gcgactggaa acctgccgcc gggcaaacct ggcatagctt
    16201 taacgattac attaatttca gcgacaaaac aggctgggat aaatggtggg gaaaaaactg
    16261 gatcagaacg gatatcggcg attacgacaa tcctggattc gacgatctca ctatgtcgct
    16321 ggcctttttg ccggatatca aaaccgaatc aactaccgct tctggtctgc cggtgttcta
    16381 taaaaacaaa acggatactc acgctaaagt catcgaaggc tttacgcctc gcgattactt
    16441 aacccactgg ttaagtcagt gggtccgcga ctatgggatt gatggttttc gggtcgatac
    16501 cgccaaacat gttgagttgc ccgcctagca gcaactgaaa accgaagcca gctccgcgct
    16561 tcgcgaatgg aaaaaaacta accccgacaa agcattagat gacaaacctt tctggatgac
    16621 cggtgaagcc tggggccacg gcgtgatgca aagtgactac tatcgccacg gcttcgatgc
    16681 gatgatcaat ttcgattatc aggagcaggc ggcgaaagca gtcgattgtc tggcgcagat
    16741 ggatacgacc tggcagcaaa tggcggagaa attgcagggt ttcaacgtgt tgagctacct
    16801 ctcgtcgcat gatacccgtc tgttccgtga agggggcgac aaagcagcag agttattact
    16861 attagcgcca ggcgcggtac aaatctttta tggcgatgaa tcctcgcgtc cgttcggtcc
    16921 tacaggttct gatccgctgc aaggtacacg ttcggatatg aactggcagg atgttagcgg
    16981 taaatctgcc gccaacgtcg cgcactggca gaaaatcagc cagttccgcg cccgccatcc
    17041 cgcaattggc gcgggcaaac aaacgacact ttcgctgaag cagggctacg gctttgttcg
    17101 tgagcatggc gacgataaag tgctggtcat ctgggctggg caacagtgac ttttccggct
    17161 tcccgttcgt cagtacctcg ggaagccgcc aaccaggata aaatgtcagc cctaatcagc
    17221 gttgcgggat aaagcaccgc tcactcttca acagaccgat ttgcacccca gcaaatgtag
    17281 cgttattgtt accttccttg ctacagagtt cgacagatat cccgctatga cattctccct
    17341 ttttggtgac aaatttaccc gccactccgg cattacgctg ctgatggaag atctgaacga
    17401 cggtttacgc acgcctggcg cgattatgct cggcggcggt aatccggcgc agatcccgga
    17461 aatgcaggac tacttccaga cgctactgac cgacatgctg gaaagtggca aagcgactga
    17521 tgcactgtgt aactacgacg gtccacaggg gaaaacggag ctactcacac tgcttgccgg
    17581 aatgctgcgc gagaagttgg gttgggatat cgaaccacag aatattgcac taacaaacgg
    17641 cagccagagc gcgtttttct acttatttaa cctgtttgcc ggacgccgtg ccgatggtcg
    17701 ggtcaaaaaa gtgctgttcc cgctggcacc ggaatacatt ggctacgctg acgccggact
    17761 ggaagaagat ctgtttgtct ctgcgcgtcc gaatattgaa ctgctgccgg aaggccagtt
    17821 taaataccac gtcgattttg agcatctgca tattggcgaa gaaaccggga tgatttgcgt
    17881 ctcccggccg acgaatccaa caggcaatgt gattactgac gaagagttgc tgaagcttga
    17941 cgcgctggcg aatcaacacg gcattccgct ggtgattgat aacgcttatg gcgtcccgtt
    18001 cccgggtatc atcttcagtg aagggcgccc gctatggaat ccgaatatcg tactgtgcat
    18061 gagtctttcc aagctggggc tgcctggctc ccgctgcggc attatcatcg ccaatgaaaa
    18121 aatcatcact gccatcacca atatgaacgg cattatcagc ctggcacctg gcggtattgg
    18181 tccggcgatg atgtgtgaaa tgattaagcg taacgatctg ctgcgcctgt ctgaaacagt
    18241 catcaaaccg ttttactacc agcgtgttca ggaaactatc gccatcattc gccgctattt
    18301 accggaaaat cgctgcctga ttcataaacc ggaaggagcc attttcctct ggctatggtt
    18361 taaggatttg cccattacga ccgagcagct ctatcagcgc ctgaaagcac gcggcgtgct
    18421 gatggtgccg gggcacaact tcttcccggg actggataaa ccgtggccgc atacgcatca
    18481 atgtatgcgc atgaactacg taccagagcc ggagaaaatt gaggcggggg tgaagattct
    18541 ggcggaagag atagaaagag cctgggctga aagtcactaa aaattgcctg atgcgctacg
    18601 cttatcaggc ctacatgatt tctgcaatat attgaatttg gaagaatttg taggccggat
    18661 aaggcgttta cgccgcatct ggcataaaca aagcgcacta ttccggcgtt gagaaacgcc
    18721 ggaaaacgtt tcaaacaggc tgctgctgta gccgatgccg ctgcactttc tcgacatcca
    18781 tgcactgcaa cgcctttgtc gggcaggctt caacacaggc cggccccgtc tcccgatgcc
    18841 agcacagatc gcacttaatc gccctcgcct ttttgcgcga agaaacgacc tccatcgcac
    18901 caaacgggca agccagcata cagcttttac agccaatgca acgtgtttgt tcaacgaaaa
    18961 tatgcccatg ttcgcggctt atcgcgtcaa cagggcagac attcgcgcac ggtgcatctt
    19021 cacactgatg acaggctact ggcgtggtcc agctgtggtc tttaatgaca cgaatacggg
    19081 aaataaactc gtctggtgac aacgcagcgc aatcctgatt ctcctgatgc gacactgcgc
    19141 aagccacttc acaggtacgg caaccgatac atttcgtcgc atccgcaata ataaaccggt
    19201 tcatgatcat tcctttgcac taaaacagca gagtgccaga gagcgcgtcc ctactgcttt
    19261 gatccaacga aggaaatgtt aaagttttgg cacttgtcca aagcgttatg tgatcgcgcc
    19321 caggtcatcc ctgacagtaa atcccagttc attagaaata gcctgcgcgg tttcacgcag
    19381 tggtttcagg agatttttct ctcccacctg tttcagacgt gatgtcgaaa gcgaaatcga
    19441 cacggcgtac ggcacccgcc catgaatatc aaacaccgga acagcaatac aggagacgcc
    19501 gagttcgttt tcttctctgt ccatcgccgc tccgctttca cgaatgtgcg ccagttcgtc
    19561 gaacatcgcg ggcagctcgg taatggtatt gcgggttaac ggctggatct catgctgatg
    19621 gttttcccag tatgacttca cgtagtccgg gtgaccaaac gccatgtaga tcttgcccat
    19681 tgcggaacag tagagcggca tatgctggcc aatataggca cgggttcgca gcatcccggt
    19741 tgtgggttcc agcttataaa tcaaaatagc gtgatcgtct tcgcggctgg agaagttaat
    19801 ggtttcacca gtggcgatgt tcagtgcctc aagatgcgga gcggcgatat gaatgatatt
    19861 cagcgaagac agcgccttct gcccgacggc aataaatttg gtagtcaggc ggtaactccc
    19921 tgcggcgggc gcggtggtca catagccgca ggactgtaat ccctgcaata agcgatggac
    19981 ggtactctta tttaaaccag ccagctccga aagatgcgcc aacggacaac cgtttggata
    20041 gttgctcaaa atctcaatca gcatcaaccc gcgaaacaga ctctggcttc cggctggacg
    20101 ctctttttcc tgcgccatct cgttctcttt ttttcccatc acttctttcc ccattttgtc
    20161 gcgtcctgat ggtagcgcaa agtgtgccgt agttcacgat ctcgacagat aatttataac
    20221 cacttgattt ttatgtcttt tgaaattcat caatcagatt gctttgttaa aaagtgatcg
    20281 atatatttga aatctagttc cgcatattga aattttaagc caaaaaagcg atcaaaaaaa
    20341 caaggaagcc taggatgaaa gtgacatttg agcagttaaa agcagccttt aatcgggtct
    20401 taatttcacg cggcgttgac agcgaaacgg ctgacgcctg tgcagagatg ttcgcccgca
    20461 ccaccgaatc cggcgtttat tctcacggcg ttaatcgttt ccctcgtttc attcaacaac
    20521 tggaaaacgg cgatatcatt cctgatgccc aacccaaacg tataaccagc ctcggcgcaa
    20581 ttgaacagtg ggatgcccag cgttcgatcg gcaacctgac ggcgaaaaag atgatggatc
    20641 gcgccattga actggctgcc gatcacggta ttggtctggt ggcacttcgt aatgccaacc
    20701 actggatgcg cggcggcagc tacggctggc aggcggcgga aaaaggctat attggcattt
    20761 gctggaccaa ctccatcgcc gtaatgccgc cgtggggcgc aaaagagtgt cgcatcggca
    20821 ctaacccgct gatcgtcgcc attccttcca cgccgatcac catggtcgat atgtcgatgt
    20881 tctcttacgg catgttagaa gttaaccgcc tggcaggccg tcagctcccg gtcgatggtg
    20941 gctttgatga tgagggcaat ttgaccaaag aacctggcgt tatcgagaag aatcgccgca
    21001 ttttgccgat gggctactgg aaaggttctg gcatgtcgat tgtgctggat atgatcgcta
    21061 ctctcctttc cgacggcgca tcggttgccg aagtcaccca ggacaacagc gacgaatacg
    21121 gcatttcaca aatttttatt gccattgaag tggacaagct tatcgacggt cccacccgcg
    21181 atgccaagct gcaacgtatc atggattacg ttactaccgc tgaacgcgct gacgaaaacc
    21241 aggccatccg cttacccggc cacgaattta ctaccctgct ggccgaaaac cgccgtaacg
    21301 gcatcactgt tgatgacagc gtgtgggcca aaatccaggc gttatgagga gataagtcat
    21361 gatttttgga catatcgcgc agcccaatcc gtgccgtttg cccgccgcca ttgaaaaggc
    21421 gctcgatttt ctgcgcgcta ccaacttcaa cgtcctggag ccgggcgttg tcgaaatcga
    21481 cggcaagaat atttatgcgc
//
LOCUS       DAOFMX010000067        21275 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.067, whole genome shotgun sequence.
ACCESSION   DAOFMX010000067 DAOFMX010000000
VERSION     DAOFMX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.067"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..444)
                     /locus_tag="QBA20_002913"
     CDS             complement(<1..444)
                     /locus_tag="QBA20_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDQ6293371.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /gene="manZ"
                     /locus_tag="QBA20_002914"
     CDS             complement(499..1350)
                     /gene="manZ"
                     /locus_tag="QBA20_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDQ6293372.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /gene="manY"
                     /locus_tag="QBA20_002915"
     CDS             complement(1363..2163)
                     /gene="manY"
                     /locus_tag="QBA20_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDQ6293373.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="QBA20_002916"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="QBA20_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDQ6293374.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="QBA20_002917"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="QBA20_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDQ6293375.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="QBA20_002918"
     CDS             complement(5214..6812)
                     /locus_tag="QBA20_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDQ6293376.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="QBA20_002919"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="QBA20_002919"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDQ6293377.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="QBA20_002920"
     CDS             complement(8491..9069)
                     /locus_tag="QBA20_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDQ6293378.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="QBA20_002921"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="QBA20_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDQ6293379.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="QBA20_002922"
     CDS             10508..10687
                     /locus_tag="QBA20_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDQ6293380.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11166)
                     /locus_tag="QBA20_002923"
     CDS             complement(10807..11166)
                     /locus_tag="QBA20_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDQ6293381.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="QBA20_002924"
     CDS             complement(11528..11872)
                     /locus_tag="QBA20_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDQ6293382.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="QBA20_002925"
     CDS             12004..13914
                     /locus_tag="QBA20_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDQ6293383.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLSNPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="QBA20_002926"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="QBA20_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDQ6293384.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="QBA20_002927"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="QBA20_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDQ6293385.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15493..17178
                     /gene="fadD"
                     /locus_tag="QBA20_002928"
     CDS             15493..17178
                     /gene="fadD"
                     /locus_tag="QBA20_002928"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDQ6293386.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPELVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17260..18375
                     /gene="rnd"
                     /locus_tag="QBA20_002929"
     CDS             17260..18375
                     /gene="rnd"
                     /locus_tag="QBA20_002929"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDQ6293387.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18429..19394)
                     /gene="yeaX"
                     /locus_tag="QBA20_002930"
     CDS             complement(18429..19394)
                     /gene="yeaX"
                     /locus_tag="QBA20_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDQ6293388.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19450..20067)
                     /gene="yeaW"
                     /locus_tag="QBA20_002931"
                     /pseudo
     CDS             complement(19450..20067)
                     /gene="yeaW"
                     /locus_tag="QBA20_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20409..20975)
                     /locus_tag="QBA20_002932"
     CDS             complement(20409..20975)
                     /locus_tag="QBA20_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6293389.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21187..>21275
                     /locus_tag="QBA20_002933"
     CDS             21187..>21275
                     /locus_tag="QBA20_002933"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6293390.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5269 a         5274 c         5461 g         5271 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta caacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcccc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgtgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatccttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgctaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcaggggttc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgggtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 tctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgacg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtcc cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaaaaag aaacagcggc tggtccgctg tttctgcatt cttacggtaa agataaaaat
    15361 aaatagtgac gcgcttcgca accttttcgt tgggtaatta tcaagctggt atgatgagtt
    15421 aatattatgt taacagcgtg tatatcattt ggggttgcga tgacgacgaa cacgcatttt
    15481 agaggtgaag aattgaagaa ggtttggctt aaccgttatc ccgcggacgt tccgacggag
    15541 atcaaccctg accgttatca atctctggta gatatgtttg agcagtcggt cgcgcgctac
    15601 gccgatcagc ctgcgtttgt gaatatgggg gaggtgatga ccttccgcaa gctggaagaa
    15661 cgcagtcgcg cgtttgccgc ttatttgcaa caagggttgg ggctgaagaa aggcgatcgc
    15721 gttgcgttga tgatgcctaa cttattgcaa tatccggtgg cgctatttgg cattttgcgt
    15781 gccgggataa tcgtcgtaaa cgttaacccg ttgtataccc cgcgtgagct tgagcatcag
    15841 cttaacgata gcggcgcatc ggcgattgtt atcgtgtcta actttgccca cactctggaa
    15901 aaagtggttg ataaaaccgc cgttcagcac gtaattctga cccgtatggg cgatcagcta
    15961 tctacggcaa aaggcacggt agtcaatttc gttgttaaat acatcaagcg cttggtgccg
    16021 aaataccatc tgccagatgc catttcattt cgtagcgcac tgcataacgg ctaccggatg
    16081 cagtacgtca aacccgaact ggtgccggaa gatttagcct tcctgcaata caccggcggc
    16141 accacaggtg tggcgaaagg cgcgatgctg actcaccgca atatgctggc gaacctggaa
    16201 caggtcaacg ccacctatgg gccgttgctg catccaggta aagaactggt agtaacggca
    16261 ctgccgctgt atcacatttt tgccctgacc attaactgcc tgctgtttat cgaactgggt
    16321 gggcagaacc tgcttatcac taacccgcgt gatattccag agttggtaaa agagttagcg
    16381 aaatatccgt ttaccgctat cactggcgtt aacaccttgt tcaatgcgtt gctgaacaat
    16441 aaagagttcc agcagctgga tttctccagt ctgcatcttt ccgcaggcgg tgggatgcct
    16501 gttcagcaag tggtggcaga acgctgggtg aaactgaccg gacagtatct gctggaaggt
    16561 tatggcctga ccgagtgcgc gccgctggtg agcgttaacc cgtatgatat tgattatcat
    16621 agcggtagca tcggtttgcc ggtgccatcg acggaagcca aactggtgga tgacgacgac
    16681 aacgaagtac caccgggtca accgggtgag ctttgtgtca aaggaccgca ggtgatgctg
    16741 ggttactggc agcgtccgga tgctacagat gagatcatca aaaatggctg gttacacacc
    16801 ggcgacatcg cggtgatgga tgaagaagga ttcctgcgca ttgtcgatcg taaaaaagac
    16861 atgattttgg tttccggttt taacgtctat cccaacgaga ttgaagatgt cgtcatgcag
    16921 catcctggcg tacaggaagt cgcggctgtt ggcgtacctt ccggctccag tggtgaagcg
    16981 gtgaaaatct tcgtagtgaa aaaagatcca tcgcttaccg aagagtcact ggtgactttt
    17041 tgccgccgtc agctcacggg atacaaagta ccgaagctgg tggagtttcg tgatgagtta
    17101 ccgaaatcta acgtcggaaa aattttgcga cgagaattac gtgacgaagc gcgcggcaaa
    17161 gtggacaata aagcctgagc gttaagtcag tcgtcagacg ccggttaatc cggcgttttt
    17221 tttgacgccc actaaagaga aaacaatttg aattaccaaa tgattaccac ggacgatgcg
    17281 ctggcttctt tgtgtgaagc cgtccgtgtc tttccggcga tagccctgga tactgaattt
    17341 gttcgtacgc gcacttatta cccgcagctg gggttgattc aacttttcga tggcgagcat
    17401 ctggcgctaa ttgatcctct cgggatcacc gactggtcac cgctgaaagc gatcctgcgc
    17461 gatccgtcca tcacaaaatt tctccatgca ggcagtgaag atctggaagt gttcctcaat
    17521 gtctttggcg aattaccaca acccttgatt gacacgcaaa tccttgctgc cttctgcgga
    17581 cgcccgatgt catggggttt cgcttccatg gtggaagagt attccggcgt tacgctggac
    17641 aagagtgaat cgcgcaccga ctggctggcc agaccgctga ccgaacgtca gtgtgaatac
    17701 gcagcggcgg atgtctggta tctgttaccg atcaccgcca agcttatggt agaaacggag
    17761 gcctccggct ggctacctgc ggcgctggat gaatgccgcc tgatgcaaat gcgtcgtcag
    17821 gaagtcgttg cgccggaaga tgcctggcgt gatatcacca atgcctggca gttacgcaca
    17881 cgccaactgg cctgtctgca actgttagcc gactggcgac tgcgcaaggc gcgagagcgt
    17941 gatctggcgg tgaactttgt cgtgcgtgaa gagcatttgt ggtcggtcgc gcgttatatg
    18001 ccgggaagtt taggcgaact ggacagcctg ggtttatccg gtagcgaaat ccgctttcac
    18061 ggtaaaacgc tgctggcgct ggtggaaaaa gcgcagacat tgccggaaga tgccttaccg
    18121 cagccgatgc ttaacctgat ggacatgcct ggttatcgta aagcgtttaa agcgattaag
    18181 tcgctgatta ctgacgtgag cgaaacgcat aagatcagcg ccgaattgct ggcatcgcgt
    18241 cggcaaatca accaactgct gaactggcac tggaaactga aaccgcagaa caatttgccg
    18301 gagctgattt ccggctggcg tggtgagctg atggcggaag cattacacaa tttattgcag
    18361 gaatatccgc agtaaaatct tccgaagccg gactgggcgc gctcagcgcc acatccggct
    18421 ttggcaaact acaaatccaa caccaggcgt ttacccttcg cacgcgaaca acagatcaac
    18481 atactttgct ggctggcacg ctcttcatcg ctaaaatact gatcccgatg gtcagcttca
    18541 ccttccagta ttgccgtttc gcaggttccg catacccctt cacgacataa acattccact
    18601 ttcgcggctt tattattttc aataacctgc aaaatagtca tctcttccgg caccacaaac
    18661 tcttttccgg aacgggcaag caccagggta aatgcatcgc cggttttgtc ttcgatagca
    18721 aattgctcaa agtgcagtgt atcggcggcg atgtccagac gcgcagcttc acttcttacc
    18781 gcttcaatta gcgcctcggg gccacaggta taaacgtgtg tgccaggttc gatatccgcc
    18841 aataatcgcg ctaattccag tcgtgttccg gttgatgaat gatgcaaatg gactttctca
    18901 gcctgcggat gctggactag ctcagcacga aatgcgcaac tttctggatt tcgcgagcag
    18961 taatgtagct gccagtcgat gtcgctgtgt tgcaactctg ccatgtgcga caggaaaggg
    19021 gtgataccaa taccgcccgc gataaacaga tgctttctgg ctgagggaat tagcgcaaac
    19081 aggttattag gcgttgagat cgttaaccga tcgcccactt ttacctgctg atgcaaaaag
    19141 cgggaaccgc cgcgcgagtt ttcttccagt cgaacggcaa tctgataact agaggtgtta
    19201 tgcggtgaac tcagtagtga atacgcattg ctgtactggt tatcaccatc gctcatctgc
    19261 actaggatgt gacttcctcc ggtgaacgca ggcagcggtt tgccatcggt tgccaccagc
    19321 gtgaagcgtt tcacctgttc ggtaaggggt tcaacctggc tcacctgtac ttcaaacatt
    19381 tgatagtctg acattgctgt actccatcaa aaatcagccc agcggcggaa aataccgccg
    19441 ccgatgtcat tagtccttaa acacctgcgc cagcagatta tggaaatggg cgataccatg
    19501 ttcggaaatg ccactaccgc tactgtcggc catgatgcgc ccctgaccac gatagccacg
    19561 cgatttcagc cctttctgta cgctttcaac cagacgtaaa tcttccggat ggaacacatc
    19621 gcgataccac tcaatcagcg atttttgctc gtcggttaac tcttcattgg tgaagtaaat
    19681 atcgtagttt tgcagggtag tttcagaatc caccgggaat tcataaatga ccgtcatcat
    19741 ccctttgatc ggggtgacgt tcagcatcgt gcacggccac agccagaaac cgtggaatgc
    19801 cgcatccgta ccctcttcaa atttaaacga ctgttcggac ggtttggcga aaccgtattg
    19861 cagcgtccag ttaccgtgca tggtgtgcca gtaacgatca acctgtacgg agtcggagaa
    19921 acctagatgc gccggaccac agtgatagca ctcgagatag ttatcgacaa tgttcttcca
    19981 gttggcaggc gtgcgggtgg taaagcgggc cgctagtttc agattgtgga cttccgggca
    20041 ggcttccagc actttcgcgc ccagaccggt gtattgattc acttgaagta cgaaaaaaaa
    20101 ccgggaggac attggattat tcgggttctg atgggattag atttggtggg gcttgcaagc
    20161 ctgtagcgca aattttagtc gttaatcaat gaaacgcgaa agatagtaaa aaattgcttt
    20221 tgtttcattg aaaatacgaa aaacaaaaac actgcaaatc atttcaataa cagcttcaaa
    20281 aaacgttcaa aaccgataac aaccaggctg taaccaaatg actcatatca caaatcagct
    20341 catgccgttt aggtatgtta catgtgtgat tatgtgaggt gaagtatgtt ttagctggtt
    20401 catggttgtt atacggcttt ttttacatct tgtggttctt gtgaaggagc tacaagacct
    20461 tcctgttcat aaataccata ctttgacaaa atctttttac gagtatctgt tggtaatgat
    20521 tcaagcattt tggctgtttg taaggtttgc tgcgaaatac cttgcaaatc agcctggatg
    20581 gaatcatcgg aagaaaaaat ctcaggcata agagcttcaa atcctttctt tctgaacaac
    20641 tcaatcgcag aatgcagttc cttgtcctct aacaattcga caattgcgat cagtttagtt
    20701 ttcaatgtgc tgcggtcagg ttgagaattt ttattctctt gattattttt atgctcaacc
    20761 attggcatgc tctgactttg gataaaccac tctattggac gccctgttgc gtaggctatt
    20821 tgtgctaatc gatctaaaga cgggtatgtc ttgccagaca ggtagtttct gataacccca
    20881 tcagaaagtc cgcacttagc tgcaaacgcc cggcttgatt ctccattcat tgcctcttca
    20941 aggcgttcct tgaaacgcat tatttccggg ggcataaaaa tgcgctctgc atttttgctt
    21001 tctttttcac ctgtcatgcc caatctctct gttatataaa gaattttaca gaattctttg
    21061 atttgcactg gttaaaataa aaatgcgcat agctcttgaa ttgagcattt ttctgcgata
    21121 tcgtttttcc cgatagcggt agccggtatc agttaccgaa aacgttaaga gagtaaggat
    21181 cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc agataaaggc tgctgttcgt
    21241 atgaagggga agacactatc tcaattatcc agaga
//
LOCUS       DAOFMX010000068        21255 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.068, whole genome shotgun sequence.
ACCESSION   DAOFMX010000068 DAOFMX010000000
VERSION     DAOFMX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.068"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            20..793
                     /locus_tag="QBA20_002934"
                     /pseudo
     CDS             20..793
                     /locus_tag="QBA20_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="QBA20_002935"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="QBA20_002935"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDQ6293391.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="QBA20_002936"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="QBA20_002936"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDQ6293392.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="QBA20_002937"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="QBA20_002937"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDQ6293393.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /locus_tag="QBA20_002938"
     CDS             complement(4991..7225)
                     /locus_tag="QBA20_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDQ6293394.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="QBA20_002939"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="QBA20_002939"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDQ6293395.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="QBA20_002940"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="QBA20_002940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDQ6293396.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="QBA20_002941"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="QBA20_002941"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDQ6293397.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="QBA20_002942"
     CDS             complement(12980..14320)
                     /locus_tag="QBA20_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDQ6293398.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NTGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="QBA20_002943"
                     /pseudo
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="QBA20_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
     gene            complement(16108..16557)
                     /locus_tag="QBA20_002944"
     CDS             complement(16108..16557)
                     /locus_tag="QBA20_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDQ6293399.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="QBA20_002945"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="QBA20_002945"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDQ6293400.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="QBA20_002946"
     CDS             complement(17357..17686)
                     /locus_tag="QBA20_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDQ6293401.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="QBA20_002947"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="QBA20_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDQ6293402.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="QBA20_002948"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="QBA20_002948"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDQ6293403.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..21245
                     /gene="ppnN"
                     /locus_tag="QBA20_002949"
                     /gene_synonym="ygdH"
     CDS             19881..21245
                     /gene="ppnN"
                     /locus_tag="QBA20_002949"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDQ6293404.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5227 a         5668 c         5073 g         5287 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctggt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctagttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc ataccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagagcta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcactac cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggtgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaact aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16861 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20521 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20581 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20641 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20701 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20761 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20821 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20881 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    20941 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21001 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21061 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21121 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21181 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21241 cgtaatctcc cctcc
//
LOCUS       DAOFMX010000069        21083 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.069, whole genome shotgun sequence.
ACCESSION   DAOFMX010000069 DAOFMX010000000
VERSION     DAOFMX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.069"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..60)
                     /locus_tag="QBA20_002950"
     CDS             complement(<1..60)
                     /locus_tag="QBA20_002950"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293405.1"
                     /translation="MASISIRCPSCSATEGVVRN"
     gene            102..1661
                     /locus_tag="QBA20_002951"
     CDS             102..1661
                     /locus_tag="QBA20_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDQ6293406.1"
                     /translation="MAALPFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTM
                     ENREVVDTYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGR
                     APARFGVYSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTR
                     DYHDNFTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLT
                     DEAIGVVDRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGIQTADNYYASVYS
                     VDQGVKRILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTP
                     MFMWWKGKLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEP
                     HKNLTWITSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYS
                     LVYTVENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKF
                     NNIKKALSEAK"
     gene            1713..2870
                     /locus_tag="QBA20_002952"
                     /pseudo
     CDS             1713..2870
                     /locus_tag="QBA20_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(2836..2958)
                     /locus_tag="QBA20_002953"
     CDS             complement(2836..2958)
                     /locus_tag="QBA20_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293407.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3161..4846
                     /locus_tag="QBA20_002954"
                     /pseudo
     CDS             3161..4846
                     /locus_tag="QBA20_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4884..7244
                     /locus_tag="QBA20_002955"
     CDS             4884..7244
                     /locus_tag="QBA20_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDQ6293408.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7301..10084
                     /locus_tag="QBA20_002956"
     CDS             7301..10084
                     /locus_tag="QBA20_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDQ6293409.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSHLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10445..11845
                     /locus_tag="QBA20_002957"
     CDS             10445..11845
                     /locus_tag="QBA20_002957"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDQ6293410.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            12001..13536
                     /gene="gadC"
                     /locus_tag="QBA20_002958"
     CDS             12001..13536
                     /gene="gadC"
                     /locus_tag="QBA20_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDQ6293411.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            13667..14986
                     /locus_tag="QBA20_002959"
     CDS             13667..14986
                     /locus_tag="QBA20_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDQ6293412.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            15284..15325
                     /locus_tag="QBA20_002960"
     CDS             15284..15325
                     /locus_tag="QBA20_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293413.1"
                     /translation="MVQLVDLARCVSF"
     gene            15363..16498
                     /gene="dosC"
                     /locus_tag="QBA20_002961"
                     /pseudo
     CDS             15363..16498
                     /gene="dosC"
                     /locus_tag="QBA20_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            16423..17040
                     /locus_tag="QBA20_002962"
                     /pseudo
     CDS             16423..17040
                     /locus_tag="QBA20_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            17298..17630
                     /gene="ddpX"
                     /locus_tag="QBA20_002963"
                     /pseudo
     CDS             17298..17630
                     /gene="ddpX"
                     /locus_tag="QBA20_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            17644..18540
                     /locus_tag="QBA20_002964"
     CDS             17644..18540
                     /locus_tag="QBA20_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDQ6293414.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            18537..19523
                     /locus_tag="QBA20_002965"
     CDS             18537..19523
                     /locus_tag="QBA20_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDQ6293415.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            19516..20442
                     /locus_tag="QBA20_002966"
                     /pseudo
     CDS             19516..20442
                     /locus_tag="QBA20_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(20498..20929)
                     /gene="osmC"
                     /locus_tag="QBA20_002967"
     CDS             complement(20498..20929)
                     /gene="osmC"
                     /locus_tag="QBA20_002967"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDQ6293416.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         5678 a         4936 c         4953 g         5516 t
ORIGIN      
        1 gttacgcacc acgccttcag tagcggagca ggaaggacat ctgatggaaa tggaagccac
       61 gcaagcacct taaaatcacc atcatacact aaatcagtaa gttggcagca ttacccttta
      121 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
      181 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      241 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      301 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      361 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      421 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      481 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      541 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      601 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      661 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      721 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      781 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      841 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt attcaaacag
      901 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      961 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
     1021 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
     1081 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1141 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1201 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1261 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1321 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1381 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1441 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1501 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1561 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1621 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1681 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1741 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1801 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1861 ctggcaatca ggtctatttt acctagcaag gcggtgaacc cactctggct ggcctggatt
     1921 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaatg
     1981 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     2041 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     2101 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2161 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2221 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2281 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2341 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2401 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2461 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2521 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2581 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2641 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2701 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2761 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2821 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2881 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2941 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     3001 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     3061 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3121 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3181 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3241 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3301 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3361 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3421 aatatccgta aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3481 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3541 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3601 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3661 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3721 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3781 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3841 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3901 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3961 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     4021 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     4081 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4141 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4201 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4261 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4321 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4381 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4441 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4501 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4561 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4621 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4681 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4741 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4801 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4861 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4921 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4981 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     5041 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     5101 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5161 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5221 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5281 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5341 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5401 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5461 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5521 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5581 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5641 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5701 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5761 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5821 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5881 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5941 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     6001 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     6061 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6121 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6181 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6241 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6301 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6361 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6421 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6481 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6541 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6601 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6661 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6721 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6781 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6841 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6901 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6961 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     7021 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     7081 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7141 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7201 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7261 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7321 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7381 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7441 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattgcagga
     7501 agaagacaat gagcgcggcg tggctcattt tgtagaacat atgatgttta acggcacaaa
     7561 aacatggccg ggtaataaag tcatcgaaac atttgagtca atgggcctgc gttttggtcg
     7621 cgatgttaat gcctatacca gctatgacga aacggtgtat caggtgagtt tgccgactac
     7681 gcagaaacaa aatctgcaac aagtgatggc aatcttcagt gaatggagta atgccgcaac
     7741 ctttgaaaaa ctcgaagtag acgctgaacg tggcgtaatt actgaggaat ggcgtgccca
     7801 tcaggatgcg aaatggcgca cctctcaggc gcgccgccct ttcctgctgg ctaatacccg
     7861 taatttagac cgtgaaccta tcggcctgat ggatactgtc gccacggtca cgccggcaca
     7921 attgcgccaa ttttatcaac gctggtatca accaaataat atgaccttta tcgtggtcgg
     7981 cgatatcgac agtaaagaag cgctggcgct gataaaggat aatttaagta agcttccggc
     8041 taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc gaaaaccacc tgcgctttaa
     8101 tatcattaat gataaagaaa accgggtgaa cggcatcgca ctctattatc gcctgccaat
     8161 ggtacaagtg aacgatgagc aaagctttgt cgaacaagct gaatggagca tgttagttca
     8221 gctgttcaat caacgtctgc aggaacgcat acagtcgggc gagttgaaga ctatttctgg
     8281 cggcactgcg cgcagcgtta aaattgcacc cgattatcag tcgctgtttt tccgtgtaaa
     8341 tgcacgagac gataatatgc aggacgctgc gaatgcatta atggcagagt tggcaaccat
     8401 tgatcagcat ggtttttctg ctgaagaact cgatgatgtc aaatctaccc gcctcacctg
     8461 gctgaaaaat gcggttgatc agcaagctga acgtgattta cgtatgctga ccagtcacct
     8521 ggcatccagc tcattaaata atacgccgtt cttgtcgccg gaagagacat atcaactttc
     8581 gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc agcagttaag
     8641 aaagaaccag gacgcatttt gggagcaaat ggtaaacaat gagcttgccg ccaaaaaagc
     8701 attgtctcct gcggctatcc tggcgctgga aaaagagtac gccaacaaaa agctggcggc
     8761 ttacatcttc ccaggcagaa atttatcgtt aacagtagac gctgacccac aggcggaaat
     8821 tagcagcaaa gaaacgctgg ctgagaatct gacatcatta acactttcca atggtgccaa
     8881 ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta caaattattg ccgtatcgaa
     8941 taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg caaataaagc
     9001 agttagcgga agcggcgttg gcgaactctc ctcttccagc ctgaaacgct ggagtgcgga
     9061 aaactcggta accatgagca gtaaagtcag tggcatgaat acgttgctct ccgttagcgc
     9121 gcggactaat aaccctgaac ctggtttcca gttgattaac cagcgaatca cccacagcac
     9181 gattaacgac aatatttggg catcgctaca aaatgctcaa attcaggcgt tgaaaacgct
     9241 cgaccagcgt ccagcggaga aattcgccca gctgatgtat gagacgcgct atgctgatga
     9301 ccgcacgaaa ttactgcaag aaaatcagat tgcacagttt actgccgcag atgcgctggc
     9361 tgccgatcgc cagttgtttt catctccagc ggatatcacg tttgtcattg tcggtaatgt
     9421 cgcagaagac aaactcgtgg cgttaattac gcgttactta ggctcaatca aacactctga
     9481 ttcgccatta gccgcaggta aaccattaac tcgcgcgacg gacaacgcat cggttactgt
     9541 aaaagaacaa aatgaacctg tggcacaggt ttcacagtgg aagcgttatg attcccggac
     9601 acctgttaat ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac tggcaaaaga
     9661 tctacgcgtt aatattcgtg aacaggcatc tggggcatac agcgtttctt ctcgcctctc
     9721 ggttgatcct caggctaaag atatcagtca tttgctggct tttacttgtc aaccagaacg
     9781 acatgatgaa cagttaacgt tagcgaatga agtgatggtt aagcgtttga ctaaggggat
     9841 cagtgagcaa gaactgaacg aataccagca aaacgttcag cgcagcctcg atatccaaca
     9901 gcgtagcgtt caacaattag cgaacactat tataaatagt cttattcaat atgacgatcc
     9961 tgcagcatgg actgagcagg agcaattgtt gaaacaaatg acggtagaga atgttaacac
    10021 tgccgttaaa caatatcttt ctcatccggt aaatacttat accggagtat tattgccaaa
    10081 ataataacag ccccgtcaac acatcgttgg cggggatttt agcaatattc gctattttta
    10141 tgtaataatt ttataaatgc gttcaaaata ataaccaagt actaatagtg atattttaat
    10201 gcctgatttt tacgtgataa ttcaggaggc acagaaagcg cataaaaata acaacataaa
    10261 acaccttgcc accacccaag aattctctat tacattgttt ttaatgaaaa aatatcattc
    10321 caacaatatc tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca
    10381 cttgcttact ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt
    10441 taaaatggat aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg
    10501 tgcgaagtct atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga
    10561 cgatgtcgca tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa
    10621 cctggccact ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc
    10681 cattaacaaa aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg
    10741 ttgcgtaaac atggttgccg atctgtggca tgcgcctgcg ccgaaaaatg gtcaggccgt
    10801 tggcaccaac accattggtt cttccgaggc ctgtatgctc ggcgggatgg cgatgaaatg
    10861 gcgttggcgc aagcgtatgg aagctgcagg caaaccaacg gataaaccaa acctggtgtg
    10921 cggtccggtg caaatctgct ggcataaatt cgcccgctac tgggatgtgg agctgcgtga
    10981 gatccctatg cgccccggtc agttgtttat ggacccgaaa cgcatgattg aagcctgcga
    11041 cgaaaatacc atcggcgtgg tgccgacttt cggcgtgacc tacaccggta actatgagtt
    11101 cccgcaaccg ctgcacgatg cactggataa attccaggcc gacaccggta tcgacatcga
    11161 catgcacatc gacgccgcca gcggtggctt tctggcaccg ttcgtcgccc cggatatcgt
    11221 ctgggacttc cgcctgccgc gtgtgaaatc gatcagtgct tcaggccata aattcggtct
    11281 ggctccgctg ggctgcggct gggttatctg gcgtgacgaa gaagcgctgc cgcaggaact
    11341 ggtgttcaac gttgactacc tcggtggtca gattggtact tttgccatca acttctcccg
    11401 cccggcgggt caggtgattg cacagtacta tgaattcctg cgccttggtc gtgaaggcta
    11461 taccaaagta cagaatgctt cctaccaggt cgctgcctat ctggcggatg agatcgccaa
    11521 actggggccg tatgagttca tctgtaccgg tcgcccggac gaaggcatcc cggcggtttg
    11581 cttcaaactg aaagatggtg aagatccggg atacaccctg tacgacctct ctgaacgtct
    11641 gcgtctgcgc ggctggcagg ttccggcctt cactctcggc ggtgaagcca ccgacatcgt
    11701 ggtgatgcgc attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga
    11761 agactacaaa gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca
    11821 gcagaacagc tttaaacata cctgataacg tttaacggta acggtgtccc gaaacgaacc
    11881 cgtttcggga taatttccaa agtctgttca ctggcattag caacggaaaa tattgttctg
    11941 aatacgcttc agaacaaaac aggtgcggtt ccgacaggaa taccgtttta gggggataat
    12001 atggctacat cagtacagac aggtaaagct aagcagctca cattactcgg attctttgcc
    12061 ataacggcat cgatggtaat ggctgtttat gaatacccta ccttcgcaac atcgggcttt
    12121 tcattagtct tcttcctgct attaggcggg attttatggt ttattcccgt gggactttgt
    12181 gctgcggaaa tggccaccgt cgacggctgg gaagaaggtg gtgtcttcgc ctgggtatca
    12241 aatactctgg gtccgagatg gggatttgca gcgatctcat ttggctatct gcaaatcgcc
    12301 attggtttta ttccgatgct ctatttcgtg ttaggggcac tctcctacat cctgaaatgg
    12361 ccagcgctga atgaagaccc cattaccaaa actattgcag cactcatcat tctttgggcg
    12421 ctggcattaa cgcagtttgg tggcacgaaa tacacggcgc gaattgctaa agttggcttc
    12481 ttcgccggta tcctgttacc tgcatttatt ttgatcgcat tagcggctat ttacctgcac
    12541 tccggtgccc ccgttgctat cgaaatggat tcgaagacct tcttccctga cttctctaaa
    12601 gtgggcacac tggttgtatt tgttgccttc attttgagtt atatgggcgt agaagcttcc
    12661 gcaactcacg tcaatgaaat gagcaacccc gggcgcgact atccactggc tatgttactg
    12721 ctgatggtgg cggcaatctg cttaagctct gttggcggtt tgtctattgc gatggtcatt
    12781 ccgggtaatg aaatcaacct ctccgcaggg gtaatgcaaa cctttaccgt tctgatgtcc
    12841 catgtggcac cggaaattga gtggacggtt cgcgtgatct ccgcactgct gttgctgggt
    12901 gttctggcgg aaatcgcctc ctggattgtt ggtccttctc gcgggatgta tgtcacagcg
    12961 cagaaaaacc tgctgccagc ggcattcgct aaaatgaaca aaaatggcgt accggtaacg
    13021 ctggtcattt cgcagctggt gattacttct atcgcgttga tcatcctcac caataccggt
    13081 ggcggtaaca acatgtcctt cctgatcgca ctggcgctga cggtggtgat ttatctgtgt
    13141 gcttatttca tgctgtttat tggctacatt gtgttggttc ttaaacatcc tgacttaaaa
    13201 cgcacattta atatccctgg tggtaaaggg gtgaaactgg tcgtggcaat tgtcggtctg
    13261 ctgacttcaa ttatggcgtt tattgtttcc ttcctgccgc cggataacat ccagggtgat
    13321 tctaccgata tgtatgttga attactggtt gttagtttcc tggtggtact tgccctgccc
    13381 tttattctct atgctgttca tgatcgtaaa ggcaaagcga ataccggcgt cactctggag
    13441 ccaatcaaca gtcagaacgc accaaaaggt cacttcttcc tgcacccgcg tgcacgttca
    13501 ccacactata ttgtgatgaa tgacaagaaa cactaaccat gtctgaatgg ttataagaca
    13561 agggagcgat aattcatcgc tccctttttc gtgcttgctg tcgtattgac ttaaccggga
    13621 taagtacgag aatgagcgca catctgttta ccggaaacca gcacatatgg atatctgctc
    13681 ccgaaacaag aaattaacga ttagaagacc agcgatactg gttgcattgg cacttttact
    13741 gtgtagttgt aaaagcacgc ctccagagtc catggtgaca ccaccagcag gttcaaagcc
    13801 accagccacg acgcaacaat cgtcacaacc gatgcgtggc atctggctgg ccacggtttc
    13861 tcgcctcgac tggccaccgg tttcctcggt taacattagt aatcccacca gccgagcccg
    13921 tgtacaacaa caggcgatga tcgacaaact ggatcatctc caacgtctcg gcataaacac
    13981 ggtctttttc caggtcaagc cggacggtac cgccctgtgg ccatcgaaaa ttttgccgtg
    14041 gtccgatctt atgaccggta agattggtga aaatccgggt tacgatccgc tgcaattcat
    14101 gctcgacgaa gcccacaagc gtgggatgaa agtacacgcc tggtttaacc cctatcgcgt
    14161 atcggttaat acgaagcccg gtactatcag ggaactgaat agcactctgt ctcaacaacc
    14221 ggcgagcgtc tatgtgcaac accgtgactg gatcagaacg tctggcgatc gctttgtcct
    14281 cgacccgggc atccctgagg ttcaggactg gatcacatca atagtcgctg aagtggtttc
    14341 ccgctatccg gtagatggcg tgcagtttga cgactatttc tatactgaat caccgggttc
    14401 acggctaaat gataacgaaa cgtaccgtaa atacggaggc gcatttgcgt caaaagcaga
    14461 ctggcggcgc aacaatactc agcagttaat tgcaaaggta tcacacacca ttaaaagcat
    14521 taagccggga gtcgaatttg gcgttagccc ggcaggcgtg tggcgtaacc gatcacacga
    14581 tccgctcggt tccgataccc gaggcgcggc agcctatgac gaatcctacg cagacacccg
    14641 tcgatgggtg gaacaaggat tgctggatta cattgctccc caaatttact ggccgttctc
    14701 acggagtgcc gcgcgttatg acgtgttggc aaaatggtgg gcggatgtcg ttaaaccaac
    14761 caggacccgc ctgtatatcg gtatcgcctt ctataaagtg ggtgaacctt caaagataga
    14821 gccagactgg atgattaacg gcggcgtacc ggaactgaaa aagcagctcg atcttaacga
    14881 tgcggtacca gaaattagcg gcaccatctt gttccgtgag gactatctga ataaaccgca
    14941 gactcaacaa gcggtcagct atctgcaaag tcgttggggc agttaaacta ccatgcttac
    15001 tttgtaacaa gccggtgctt tacccaccgg cttgttacac cttgtgaaat atcccactag
    15061 taatcatgct tacataagtc aaattaacca cgcaattaac cacacttagt taaacagcta
    15121 taaatcccta ataaaaatgt atgaatatga aaaaccatac gatattcgct gtcaccgaaa
    15181 aataattacg atgggctata ctcttctgaa gaacagtagc gtagattgaa actgtcttaa
    15241 gtcgcttatt tcttttcagt atatcttcat atttcaggag aatatggtgc agttagtcga
    15301 tctcgcacgt tgcgtttcgt tttgattccg tctgatttcc cagccttata agggtgtgaa
    15361 ttatggagat gtattttaaa agaatgaaag atgagtggac cggacttgtc gaacaggcag
    15421 atcctctcat tcgtgctaaa gccgcagaaa ttgcccttgc gcatgctcat tatctgagta
    15481 tcgagtttta tcgaattgtc cgcatcgacc cgcatgccga agaattcttg agtaatgaac
    15541 aagttgagcg gcagttgaaa agtgcgatgg aacgctggat tattaacgtg ctttctgccc
    15601 aggttgacga tgtcgaaagg ctaatacaaa tccagcatac cgtcgcggaa gtgcatgccc
    15661 gcataggaat tccggtagaa attgtcgaga tggggtttcg ggtgctgaaa aaaatcctct
    15721 atccggtaat cttctcttcg gattattccg ccgcagaaaa acttcaggtc taccatttct
    15781 cgattaacag tattgatatc gcgatggaag tgatgacccg cgcgtttacc tttagtgaca
    15841 gtagtgcctc aaaggaagat gaaaactatc gtatcttctc gttactggaa aacgccgaag
    15901 aagaaaaaga acggcaaata gcctcaatac tttcatggga aatagatatt atctataaag
    15961 tcctgctgga ttctgattta ggcagcagtt tgcctttaag ccaggctgat tttggcctgt
    16021 ggtttaacga cattatttta gcggcattgc cgaagtgggc catatctccc gtctgattca
    16081 ggatttcgac ggtattttca atcaaaccat gcgtaacacc aggattttga ataacagaag
    16141 tttgcgggtg aaatttctat tacagataag aaataccgta tcgcagatta ttaccttgct
    16201 gcgtgaattg tttgaagaag tatcgcgcca cgaagtcggt atggatgtac tgacgaaatt
    16261 acttaaccgc cgtttcctgc cgactatctt caaacgcgaa attgcccatg ccaaccggac
    16321 cggtacaccg ctgtcagtgc tgattattga cgttgataaa ttcaaagaga tcaacgatac
    16381 gtggggccat aacactggcg atgaaattct gcgtaaagtc tcattcctct cgcagaagag
    16441 attggtgaaa tcgaaaatat tgggcgctgg gtcatcgcgg aagcttgccg tcagttagca
    16501 gaatggcgta gccagaatat tcatatcccg gcgttatccg tgaacttgtc ggcgctgcac
    16561 tttcgcagta atcagctgcc taatcaggtg tctgatgcaa tgcaagcctg gggtattgac
    16621 ggccaccagc tgacggtgga aatcacggaa agcatgatga tggaacacga taccgaaatc
    16681 tttaagcgca ttcagatcct gcgtgatatg ggcgtgggtt tatcggtaga tgattttggc
    16741 acgggctttt ccggattatc ccgcttagtc agtcttccgg taacggaaat caaaattgac
    16801 aaaagctttg tcgatcgttg tctgacggaa aaacgcatcc tggccttact tgaagccatt
    16861 accagcattg ggcaaagcct caatttaacc gtcgtggcgg aaggggtcga aaccaaagag
    16921 caatttgaga tgctacgtaa gatccactgt cgcgttattc agggatattt cttttcccgc
    16981 ccccttcccg ccgaagaaat tccaggctgg atgagcagcg tgttaccgct gaaaatctga
    17041 caaattcctc tcgcccgcac tcgcgggttt tcctttaagg tgacactgtc acttaaatac
    17101 tgtgatttca gctacgattc cgggagattc cttccttaac tttccctctt gcaccaaaac
    17161 tgcactacgc tgcacaaatg ttgatcaaaa gttaaaaaat ataactttaa gccagcattc
    17221 tggcgcttat cccggcatgg catgagatct gcataagcgg aaagcgcagc aatttttgtc
    17281 ttatacagga accgtttatg tcggatacca ccgaactggt tgatttagcc gtgatcttcc
    17341 ctgatctgga gatcgaattg aaatatgcct gcgttgataa catcacaggt aaagctattt
    17401 atcagcaagc gcgttgtctg ctacataaag atgcgattac cgcgctggcg aaaagtatca
    17461 gtatcgccca gctgtcaggc ttacaactgg tgatttacga tgcgtatcgc ccacaacaag
    17521 cacaggcgat gttgtggcaa gcctgcccag acccgcaata tgttgttgat gtgacggtcg
    17581 gttctaatca cagccgtggc acggcgctcg acctgacgct tcgtgatgac gtggaggtgg
    17641 tgaatgatgc taagcgagga aacgtccgcc gtacgcccac aaaaacaaac gcgatttaac
    17701 ggtgcaaaac tggtatggat gctgaaaggc agtccgctca ccgtgaccgg cgcagtcatc
    17761 attgtattaa tgcgactgat gatgattttt tcaccgtggc tggcgacgca tgatcccaac
    17821 gccattgatt taaccgcccg ccttttgccg ccttctgcgg cgcactggtt tggcaccgat
    17881 gaagtgggac gcgatctgtt tagccgcgta ctggtcggca gtcagcaatc aattctcgcc
    17941 ggattagtgg tggtcgccat tgcgggtatg attggttcgc tactcggatg tctatccggt
    18001 gtgcttggcg gacgcgcaga cgccatcatc atgcgcatca tggacattat gctgtcgatt
    18061 ccttcgttgg tactgacaat ggcactggct gccgctctcg ggccgagttt atttaacgcc
    18121 atgctggcga ttgctattgt gcgaattccc ttttatgtgc gcttggcgcg ggggcaagca
    18181 ttagttgtac gccagtatac ctatgttcag gcggcgaaaa cctttggcgc gtctcgttgg
    18241 catctgatca actggcatat tttacgtaac tccctaccgc cgctgatcgt gcaggcatcg
    18301 ctggatatcg gtagcgcgat tttaatggcc gccacgttgg gatttattgg cctaggtgct
    18361 caacaaccga gtgctgaatg gggcgcgatg gtagcgaatg gtcgcaacta tgtactcgat
    18421 cagtggtggt attgcgcatt tccgggggca gcgattttgc ttacctccgt cgggtttaat
    18481 ctctttggtg atggtattcg cgatctgctt gacccgaaag caggaggaaa gcagtcatga
    18541 cccaacccgt tctggacatt caacagctgc atttgagttt ccccggtttt aacggtgatg
    18601 ttcacgcgct caacaatgtg tccttgcaga ttaaccgcgg cgaaattgtc ggtctggtgg
    18661 gagaatccgg ctcaggtaaa tcagtcaccg caatgctgat tatgcgtctg ctaccgacgg
    18721 gcagttattg cgtacatcgg gggcagattt cactgctggg agaagatgtt cttaacgctc
    18781 gggaaaaaca gcttcgtcag tggcgcggcg cacgagtggc gatgatcttt caggaaccga
    18841 tgaccgccct taatccgaca cgtcgaatag gtcttcagat gatggacgta atccgccatc
    18901 atcaaccaat aagtcgtcgg gaagccagag ctaaagcgat tgccctgctg gaagagatgc
    18961 aaatcccgga tgccgtggaa gttatgtcgc gctatccgtt tgagctttca ggtggtatgc
    19021 gccagcgggt aatgattgcg ctggcattct cctgcgagcc gcaattgatt attgccggcg
    19081 aaccgacgac ggcgctggac gtcacagtac agttgcaggt actgcgtttg cttaaacata
    19141 aagcacgcgc cagtggaact gcggtactgt tcatcagcca tgatatggcc gtggtgtcgc
    19201 aactgtgcga tagcgtttac gtgatgtatg ccggaagcgt aattgaaagc ggcgggacgg
    19261 cagacgttat ccatcatccc cggcatccgt ataccattgg tttgctgcaa tgcgcaccag
    19321 aacatggagt accacgccag ccattacccg ccattccagg gacggtacca aacctcaccc
    19381 atttgcctga cggctgcgct tttcgcgatc gttgctatgc ggcaggtgca cagtgtgaaa
    19441 acgtcccggc gctgacagcg tgtggtgaca acaaccagca ctgcgcctgt tggtatccgc
    19501 agcaggaggt cataagtgtc tgacacgtta ttaacgttac gcgacgtcca tatcaatttt
    19561 ccggcccgta aaaactggct tggtaaatct acggaacatg ttcatgccat taatggtatt
    19621 gatttacaga tccgccgtgg tgaaacctta gggatcgtcg gcgagtcagg ctgcggcaaa
    19681 agcaccctcg cacagctttt aatgggcatg ctgcaaccga gccacgggca gtacatccgt
    19741 tcaggctcac aacgcattat gcagatggtg tttcaggacc cgctctcttc gcttaatccg
    19801 cgcttaccgg tgtggcgcat catcacagaa tcgctctgga tagctaagcg tagtagtgaa
    19861 caacagcggc gagcgttggc agaggagttg gctgtgcagg tgggtattcg tccggagtat
    19921 ctcgaccgcc tgcctcatgc gttctccggc gggcagcggc aacgcatcgc cattgccaga
    19981 gcactctctt cgcagcctga cgtgattgtg cttgatgagc caacctcggc gctggatatc
    20041 tccgtgcagg cgcagatcct caatttgctg gtaacgctac aggaaaatca cgggctgact
    20101 tatgtgctga tttcacacaa tgtctcggtg atacgtcata tgagcgatcg ggtggcggtg
    20161 atgtatctcg ggcagattgt agaactgggc gacgcgcaac aggtgttgac tgcttcggca
    20221 catccataca cccgattatt gctggattcc ctccccgcca ttgataaacc gctggaggaa
    20281 gaatgagcat tacgtaaaac ggatctgccg ggaaaccgca cgttgccgca aggctgtttt
    20341 ttctacgaac gttgccctct ggcaacccac ggatgtgaag tccggcaatc attagcaata
    20401 agggaggacg gacgtgagct ccggtgctgg cgggcgctgt agagatgcta acaacgcatc
    20461 aggcattcac gcgcctgacg cgtcatccgg caatgcttta cgatttcaac tggtaatcca
    20521 gcgtaatttc cgctttcagt acctgagaga ccgggcatcc tgcttttgct ttctggatga
    20581 tgccgtcaaa ggtagaggca tcaatacccg gcaccgcaac ttcactcttc agtgcgattt
    20641 tcgtaatcgc aaaaccggca tccactttat ccagcgacac atcggcggtg gtatcaattg
    20701 atgttggcgt gaatcccgct tcccccagca ttaatgaaag cgccattgag aaacatgcgg
    20761 catgcgctgc gccaatcagt tcttcagggt tggttccttt ttcgccttca aaacgcgtgt
    20821 taaatccata cggctgttgg ttcagcacgc cgctctcggt ggatactgtt cccttcccgc
    20881 gtttgatatc gccttcccag tgtgcctgac ctttcttatg gattgtcatt gttgctctcc
    20941 tgtgggctta aaaaggaaat tatagactac gtgagaaaca gcacgaggat aagcaggata
    21001 ttccgaataa aatacggcga aaatattact tctgcactta aattaaattc ccacaataga
    21061 taaccgggaa tatatatctc aag
//
LOCUS       DAOFMX010000070        20895 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.070, whole genome shotgun sequence.
ACCESSION   DAOFMX010000070 DAOFMX010000000
VERSION     DAOFMX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.070"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="QBA20_002968"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="QBA20_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDQ6293417.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="QBA20_002969"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="QBA20_002969"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDQ6293418.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="QBA20_002970"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="QBA20_002970"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDQ6293419.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="QBA20_002971"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="QBA20_002971"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HDQ6293420.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="QBA20_002972"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="QBA20_002972"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDQ6293421.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="QBA20_002973"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="QBA20_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDQ6293422.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6798)
                     /gene="nth"
                     /locus_tag="QBA20_002974"
                     /pseudo
     CDS             complement(6164..6798)
                     /gene="nth"
                     /locus_tag="QBA20_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(6798..7493)
                     /gene="rsxE"
                     /locus_tag="QBA20_002975"
     CDS             complement(6798..7493)
                     /gene="rsxE"
                     /locus_tag="QBA20_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDQ6293423.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7497..8117)
                     /gene="rsxG"
                     /locus_tag="QBA20_002976"
     CDS             complement(7497..8117)
                     /gene="rsxG"
                     /locus_tag="QBA20_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDQ6293424.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8121..9179)
                     /gene="rsxD"
                     /locus_tag="QBA20_002977"
     CDS             complement(8121..9179)
                     /gene="rsxD"
                     /locus_tag="QBA20_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDQ6293425.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9180..11402)
                     /gene="rsxC"
                     /locus_tag="QBA20_002978"
     CDS             complement(9180..11402)
                     /gene="rsxC"
                     /locus_tag="QBA20_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDQ6293426.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(11395..11973)
                     /gene="rsxB"
                     /locus_tag="QBA20_002979"
     CDS             complement(11395..11973)
                     /gene="rsxB"
                     /locus_tag="QBA20_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDQ6293427.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11973..12554)
                     /gene="rsxA"
                     /locus_tag="QBA20_002980"
     CDS             complement(11973..12554)
                     /gene="rsxA"
                     /locus_tag="QBA20_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDQ6293428.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12631..13071)
                     /locus_tag="QBA20_002981"
     CDS             complement(12631..13071)
                     /locus_tag="QBA20_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDQ6293429.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13157..13372)
                     /gene="ydgT"
                     /locus_tag="QBA20_002982"
     CDS             complement(13157..13372)
                     /gene="ydgT"
                     /locus_tag="QBA20_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDQ6293430.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13645..13770)
                     /gene="blr"
                     /locus_tag="QBA20_002983"
     CDS             complement(13645..13770)
                     /gene="blr"
                     /locus_tag="QBA20_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDQ6293431.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            14013..15053
                     /locus_tag="QBA20_002984"
     CDS             14013..15053
                     /locus_tag="QBA20_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDQ6293432.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(15089..16090)
                     /gene="add"
                     /locus_tag="QBA20_002985"
     CDS             complement(15089..16090)
                     /gene="add"
                     /locus_tag="QBA20_002985"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDQ6293433.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16194..17366)
                     /gene="malY"
                     /locus_tag="QBA20_002986"
     CDS             complement(16194..17366)
                     /gene="malY"
                     /locus_tag="QBA20_002986"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDQ6293434.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17376..18968)
                     /gene="malX"
                     /locus_tag="QBA20_002987"
     CDS             complement(17376..18968)
                     /gene="malX"
                     /locus_tag="QBA20_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDQ6293435.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19143..20171
                     /gene="malI"
                     /locus_tag="QBA20_002988"
     CDS             19143..20171
                     /gene="malI"
                     /locus_tag="QBA20_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDQ6293436.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            20283..>20895
                     /gene="hdhA"
                     /locus_tag="QBA20_002989"
     CDS             20283..>20895
                     /gene="hdhA"
                     /locus_tag="QBA20_002989"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDQ6293437.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PSDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITPE"
BASE COUNT         5324 a         5262 c         5363 g         4946 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttga
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttc cccggcgcaa attgagtacg
     6361 attacaaacg cggaaaatgt gcgtgtcgac agcaatagtc ggccagccga atgcagtgtt
     6421 taatacgacg ttggctgttt tacgacctac gccgggcagg gcttcaagcg cagcacgatc
     6481 ttccggaacc tcgccattat gttgttccag taatatacgg caggttttga tgatattttc
     6541 tgctttgctg ttataaaggc caatcgtttt gatataggtt ttcacccctt caacgcctag
     6601 ttcaagcatc gctgcaggcg tattcgccac cgggtagagt ttcgccgtcg ccttattaac
     6661 actgatatcg gtcgcctgag cggaaagcag tacggcaatc agcaattcaa aaggcgaact
     6721 gaaattaagc tcggtggtgg gatgaggatt gttctcacgc aggcgagtga ggatctccag
     6781 acgttttgct ttgttcatca gacattccct gtttcaccgt ttggcaatgc acgttcggca
     6841 gctgcttcag cacggcgctt tttcattctt tcatcaatca ggtattttcc tgccagcatc
     6901 agtcccaagc caataaatgc acctggtggc agcatcgcca gcaggaaagg ggagtcggtg
     6961 tggaaaatct ccacgcgtaa tacttttgcc cagctaccta acagcgcatc tgcaccgtca
     7021 aacaacgtgc cattgccgat aatttcgcgt agtgaaccca gcacgaacat ggcgcaggtt
     7081 gcgcccgtac caattgaaaa gccgtccagt gccgaaagcg ccggaccttt tttggcggcg
     7141 aaggcttcag cgcggcccac aacgatacag ttagtgacaa tcagcggaat aaaaatccct
     7201 aatgattgat acaggccaaa ggcgtaggcg ttgatcagca tctgtacagc gctgaccacc
     7261 gaggcgatga tcatcacgta aatgggaatg cggatctcgg ctggcgtcca gtgacgcagc
     7321 gtcgaaatgg tcaggttggt cagcgtcagt accagcgtag tcgcaagtcc taaacccaga
     7381 gcgttagtgg cagtggatgt gaccgccaat agcggacaaa ggccgagcag ttggaccagt
     7441 gcagagttgt ttttccacaa cccctgaaca ataacgtctt taatttcgct cacgctttat
     7501 tctccacagg caggaagttg agaaagttgt gccggtaacg tctgagcgta caatccggcg
     7561 cgttttaccg cattaaccac cgcgcgggga gtaatcgtcg cgccggtgaa ctggtcgaaa
     7621 tcaccaccat ctttcttcac cgcccagtgc gcatcatccg caccactgat tttttttccc
     7681 gcaaaatggg tgagccagtc agaaaggcgc aattcgattt tatcgccaag ccctggcgtt
     7741 tcgtggtgct ctgtcacgcg cgtgccaagt accgtgccat taaaatcggc tcccaccagc
     7801 agctgaatcg caccggaata gccatctggc gcggttgctt ccagaacggc ggctaccggt
     7861 ttgtcatcct gtttggcgat gtaaacccga tgctcacctt tacctaactc tggcgcagtt
     7921 accagatagc aactctgtgc gagcgcattg ttatagcgtt cggctggcag cacctgatca
     7981 aataacgtct tttgttgcag actggcctgt tcagcaattg tcgttttggt catctggttg
     8041 atggccgcag ttaaccctgt agaacccgct gcaaatagtg ccagtgtaat gccgtgtttt
     8101 cggatagttt tcagcatggt ttaccctttg cgatggccgt agacgcgcgg acgcgtgtag
     8161 taatcgatca gaggaaccgt gatgttcgcc agcaggacgg caaaagccac gccgtcagga
     8221 tagccgccga aactgcggat caaccagact aataagcccg ccagcgcgcc gaacatcagc
     8281 cgaccacgat tggtcgtaga agcagtaact gggtcagtca aaataaagaa tgcgccgagc
     8341 atagtcgcac cagacagcag atgaatttgc ggtgctgcca gtgtttctgg tgagaacaac
     8401 cagcccaacg ttgcgcataa cgccagcgtt actaagaagc tgaggggaat atgccagcga
     8461 atcgctttct gccatagcaa ccacacgcca ccagccagcc aggcgagatt tacccattgc
     8521 caaccagcgc ccgccagaat accgctgtag atcggatatt gcataatctg ttcaaccgaa
     8581 tgacctgcgc ggacagaggt tttgaatgta tccagcggtg tcgcctgact aatgccatca
     8641 atacctaagc gtagcgtatt catatcacca ccactggcgg tatgaccgct gaaaataacc
     8701 tgtatggcgt cgataaaacc agggatgttg accgcaattt catgtggcgg taaccagctg
     8761 gtcatctgca ccgggaagga gatcagtaag accacataac caatcattgc cggattaaac
     8821 gggttttgcc ccagaccgcc atacaactgt ttagcgatga tcaccgcaaa caccgtaccc
     8881 agtacgacca tccaccatgg cgctaggggg ggaatactta ccgccagcaa taagcctgtc
     8941 agcaatgctg agttatcttt caacgttgcg gctaccgact gcttgcgtag tttgagtacg
     9001 agagcttcgg ctaacagagc actgaccgac gccaacagga tctgaacgag agtaccccaa
     9061 ccaaaaaacc acagttgcgc tgcgattcct ggcacggctg cgagcaacac caacagcata
     9121 atgcgcgatg tctggcgctg gttatgggta taaggggagc tagctattct gaataccatt
     9181 tagtcctcgt ttacaacctt ttgctgggcg gcttttttgg cctgaacgcg ggcaatagcc
     9241 gcggcaactg cggctttgcg cggatcgacc tgttcttctg gttccgcatt agcctgttgc
     9301 tgttccagct tgcgtgcttt ggcgcgggca atagcggctt cgacggcagc tttgcgcgga
     9361 tcaacctgtt cttctggctc cgcattggcc tgttgctgtt ccagcttgcg tgctttggcg
     9421 cgggcaatag ccgcttcgac ggcagctttg cgcggatcaa cctgttcttc tggctccgca
     9481 ttggcctgtt gctgttccag cttgcgtgct ttggcgcggg caatagcggc ttcgacggcg
     9541 gctttgcgcg gatcgacctg ttcttctggt tccgcattag cctgttgctg ttccagcttg
     9601 cgcgctttgg cacgggcaat agcggcttcg acggcggctt tgcgcggatc gacctgttct
     9661 tctggttccg cattagcctg ttgctgttcc agcttgcgcg ctttggagcg ggcgatagct
     9721 gcttcaacgg cagttttacg tggttcagca acggttgctg cgtcgttagt ttgctccagt
     9781 tctgcctgtt tcgctctggc ttgcgcttta cgggcttccc gttctgcaat aattgcactg
     9841 ttatccgggc gttcgcccgc tttaatcaca ataggctgtg tagcctgggc ctgtttctct
     9901 ttcacccgcg ccagagcggc agcaatcgca tctttatctt tggctgcagg ttgaacggct
     9961 gcgctcttat gtcgttcaag gcgagtcgct ttttcgcgct ccagacgagc ctggcgcgct
    10021 tcgaaacgcg ctttggcttc tgcggcgcgc ttttcttcct gacgaatagc cgcaatttca
    10081 gctttttcct gacggaaata ttgcaccaag ggaatattgc tcgggcaaac ccacgcgcaa
    10141 gccccacatt caatgcagtc ggcaatgtta tgcgtggtag ctttatcgtg ttgctgacct
    10201 ttgctgaacc agtacaactg ttgcggcaaa agatccacag ggcaggcgtc agcacaggcg
    10261 ctacaccgga tgcagctttg ttcttcctgt ggttcgccaa gttcattggc agagggagcc
    10321 aacagacagt tggtaatctt tacgaccggg acatccagcc atggcaaggt aaagcccatt
    10381 agcgggccac ccataatcac catttgatcg gcagaggggc agaatccggc atcattcaat
    10441 aaatgacgca ctggcgtccc cagccgtgcc cagacgttgc ccgggcgagc gattgcttcg
    10501 ccagtcaggg ttacaacacg ctcggtaatc ggctcgccat caataacggc acgtttcact
    10561 gcataagcag tgccgacgtt ttgcattaat acgccgatat cggatgaacg cccgccatgt
    10621 ggaacctgct tcccggtcag aatgtaggtt aattgtttag caccgccaga aggatatttg
    10681 gttggaatca cccgcagaga aatatcgtta gagtccgcca gcaccgcgcg cagcatggaa
    10741 atcgcctgcg gtttgttatc ttcaatgccg ataagaattt cgcgtggctg cagaatatgc
    10801 gcaagaatgc gaataccctc tacgacctga gccgcgcaat cctgcatcaa acggtcatcg
    10861 gcggtaatgt acggctcgca ctcagcagcg ttgataatca acgtttcaat cttatctcca
    10921 ccaccctgca atttaacgcc tgtcgggaat ccggcaccgc ccagcccggc aacaccaaac
    10981 tgatgtatgc gctcgattaa ctcttcgcga ctgcgagagc gataatcgac ccagccgtcg
    11041 cgcgggatcc agcagtcttc accatcggca tcaataatca cgcttaattc agctaaagct
    11101 gaaggatgag ccgtagagtg gggcgcaata gccgtaacgg tacccgaggt gggcgcgtga
    11161 acaggcagca ttttgccgcg accacgggta agcggctggc cgcgcaatac tttatcgccg
    11221 acgctaacgc acaactcacc ttcagcgcca atatgctgtt tcagtggaat aacaaaatgc
    11281 tgcgccagta gtacctggcg caggggggta ccgttggtct gggttttcat ctccggtgga
    11341 tggatgccgc cgttgaaatc ccagatttta ttttttctga atgcagagaa taacttaagc
    11401 atggtgttcc acgggaatga tacgcacggg aatggtgttc agatcccatt tccaggagtc
    11461 aggtgtttct gcgaccggtt gcaacgagat gcagtgcgtc gggcacggat caacacataa
    11521 attgcagccc gtacagagat cactcattac cgtatgcatg acacgggtag cgccaacgat
    11581 ggcgtctacc ggacaagcct gaatacattt agtgcagcca atacagttat tttcatcaat
    11641 aaccgccacc atccgcgcag gcgttagctc ttgcgcttcg ccatccagcg gctgcggctc
    11701 gacattaagc aactcggcaa tttttagcat cacagcttcg ccacctgggg cgcaacggtt
    11761 gattttttca ccgttacagc tgatggcttc cgcgtagggg cgacagccgg gataaccgca
    11821 ctgaccacac tggctctgcg gtaagatttc gtcaattttc tcaacgaccg gatcgtcttc
    11881 caccgcaaaa cggcgggagg cataacccag aatggcgcca aacgccaggc ccagcaggct
    11941 cacggcggca acggcaatcc agatagcatt cattacaact tcaccaaacc actaaagccc
    12001 ataaaggcca gagacataag acctgcggta attaacgcaa tggcattacc gcgaaaaggt
    12061 gccgggacat cagccacagc aaggcgttcg cggatggcgg cgaagagcac catcaccagc
    12121 gagaaaccga cagcggcgga aaaaccgtac agcgccgact gcaagaaatt gtgcccgaga
    12181 ttgatattca gcaacgccac gccgagcact gcacagttgg tggtgataag cggcaaaaaa
    12241 atccccagca gacggtaaag caccgggctg gttttgcgca ccaccatctc ggtgaactgc
    12301 acgaccacag caatcaccag aataaatgcc agggtgcgca ggtaaatcag attaagtggg
    12361 atcaaaatcc acgtatcgat aagccaggcg caaatagacg tcagcgtcat cacaaacgtt
    12421 gttgccagcc ccatgcccat cgcggtttcc agttttttgg aaacccccat aaacggacag
    12481 agaccgagaa acttgaccag tacaaagtta ttgaccagta cagttccgac aaacagtaac
    12541 aggtagtcag tcatttttcg gcctgaaata aaaaagccgc ctattatccg ttaatccaga
    12601 gcaggcgaca acaggttaac tgtaaggtta ttacggattc acaaacgtcg cttttacccg
    12661 cgacgagcgt ttgaaatagg ggacgatcaa tgcggttgcc aacaaagtaa acagcaactg
    12721 gcgaacggct atgccaactt ccacgggtga aaaagcgaat gcttttactg ccagcaatac
    12781 ggaaataagc agccagataa tatagtgttt aggaacacaa cgccgacgtt taaagaaagc
    12841 gatggtcaac cacagtgtgt aataccacat ggcgatagcg gtaataaaag aaacgcccca
    12901 taagatttgt gtcgtcagtg cttgtccgcc gagcgtttga aatgtttgcg gagaagataa
    12961 cgcagcggta tacagcaaca acgccagcgt cgtactcaac agagcgacta aaagccaggc
    13021 caacgggcca agcaaccagc ctccaatacg ttgtggcgtt gtggtggtca taggttctcc
    13081 agaatagcga cgcaaacagg caaatctaat tttaggggcg ttattataag gcttttataa
    13141 ccgatcgcta ctttctttat tggacatagt gccagacgga cttcggtacc tggccgaggt
    13201 caaacaaacg cccgccagaa accagctctg cgcgacggtg atcggcagca cgatacatat
    13261 tgatcagttc ctgatcgtcc gtcagggtgt aattaagatg gtcgtagagt ttttccagac
    13321 tttcgagtga actgattttg cgaaatttta ataagtagtc ctgaacagtc ataaaaggtc
    13381 cataaaataa ttaataccaa aggggtaggt attattagtg atgataaaat ttcaccaaaa
    13441 gtgaacgctt gtgttattac aataataaga atgcgctgat ggcaaatatt cagggatgaa
    13501 aaactgctct gaaaagagta acgcgcttta ctatccctga caatcaccaa caacatcgaa
    13561 caagataata aattcctggt ttaatatccg acaagtgaaa acatgcaccc ggacgggcag
    13621 catgtcgctc cacaagtgca gagcttactt gtgttgtacc gaagcactct gttcaggtgg
    13681 ctgatagttg tcaatgtgac tcgccacgcc aagaagaatg actgaaacga caagaacgat
    13741 ccaacctgtt aattcaataa gacgattcat tacagcccac atctctcttg attgatccat
    13801 taacttcagg gggtaaatgt tacttagcaa taatagctcc agcaagattt ttactgaggt
    13861 ttttgcgata tttagctttt gtcgttggaa aattcgctat ttttttgact taagttaaac
    13921 aacatccctt attgctggca ggttattaaa ctgttgagcg tgggtaaagg atagtgtcaa
    13981 atagccatca tacttcaatg agaggcaatg acatgagcga caacatccgt gttgggttga
    14041 ttgggtatgg ttatgcgagc aaaaccttcc atgcgcccct gattgcgggc acgcccgggc
    14101 tggaactggc ggtaatctcc agcagtgatg aaacaaaagt aaaagccgac tggccaacgg
    14161 ttacggttgt ctctgagccg aagcatctgt ttaacgatcc caacatagac ctgattgtca
    14221 ttcctacacc caacgatacc catttcccgt tagccaaagc ggcgcttgag gcgggtaaat
    14281 atgtggtcgt tgataaaccc tttaccgtga cactgtcaca agcgcgagag ctggatgcgc
    14341 tggcaaaaag cctggggcgt gtgctgtctg tattccataa ccgtcgttgg gatagcgatt
    14401 tcttgacgct aaaaggtttg ctcgcggaag gcgtgctggg tgaagttgct tactttgagt
    14461 ctcattttga ccgcttccgt ccgcaggtgc gcgatcgttg gcgtgaacag ggcggcccag
    14521 gcagcggtat ctggtacgat ttagcaccgc atcttcttga tcaggccatt acgctatttg
    14581 gtttaccggt cagcatgacg gtagatttgg cacagttacg gcccggagcg cagtcgaccg
    14641 attatttcca cgccatcttg tcctatccac agcggcgagt cattttacac ggtaccatgc
    14701 tggcagccgc tgagtcagca cggtatatcg tgcatggatc ccgaggcagt tatgtgaaat
    14761 atggcctcga tccacaggaa gaacgtctga aaaatggcga gcgtctgccg caggaagact
    14821 ggggctacga tatgcgtgat ggcgtactta cccgcgtgga aggtgaggaa cgtgtcgaag
    14881 aaacgctgtt gacggtgcct gggaattatc cggcttacta tgcggcgatt cgtgatgcgt
    14941 taaatggcga tggtgaaaat ccggttccgg caagccaggc aatccaggta atggagttga
    15001 ttgagctggg catcgaatcc gccaaacatc gcgcgacgtt gtgccttgcg tgaaaaatgg
    15061 atggtgcaat ttgcaccatc catttttgtt acttcgcggc gactttttct cgcagtgcgc
    15121 gtttttcctc tgcgctgagg aaagccattt ccagaccatt aatctgtgcc tggcggattt
    15181 gctcacggga taacccagca gctggcgcgg caacggtata ttcgtgaatg atatccactc
    15241 cctgtacgcc gggatcgtca gtgttaatgc tggcacgaat gccatgctca aggaacgttt
    15301 tcagcggatg tgcagccagc tctgctacgg tgctggtctg aatattggag gtcagacagg
    15361 attcaatacc aatttgttgc tcggcgagaa aatccatcag cgcccgatct tcaatggctt
    15421 ttacgccatg tccaatacgc tccgcaccca gttcacgaat cgcctgccag atgctttccg
    15481 gcccggcagc ttcgcctgca tggacggtaa tatgccagcc cgcatcacgc gcgcggttga
    15541 agtgagaaag gaacagactt cccgggaatc caagttcatc accagctaaa tcaagtgcgg
    15601 taatctggtc acggtgggct aaaaaggcct ccagctcttg ctgacaggcg gcttcgccga
    15661 aggttcggct cataatgccg ataagcttcg cctgcacacc aaaggtgcgg caaccttcac
    15721 gtacgccatc gatcaccgct tcgacaacac ccgctacagg cagcttatgt gccattgcca
    15781 tgtagcctgg tgaaaaacgc agctcgacat agcgcaggcc gttacgggct gcatcttcaa
    15841 tgttttcaaa tgccacgcgg cggcaggcat caagagaggc gagaacttta acgccccagt
    15901 caagtttggt cagaaagctc accagatcgg gttcgttggc aatgacctga acgtggggaa
    15961 tcagtgtttc aagggattgt gcaggaagcg agatattata ctggcggcca agttcaagaa
    16021 tggtctgggg acgaatgttg ccatcaaggt ggcgatggat atcagttaat ggcagggtgg
    16081 tatcaatcat ggtcgcactc tttttctgga tataaaagtg cgcaccatta taaaaacaaa
    16141 gcagggtaaa aatccacgat tgcgcaacga ttttttacgt tgcgcaattg gggttagcga
    16201 acagcgcgga tggcgtttat taatccagcc acaccttttt ccagtttcga acgtgggcag
    16261 ccggcattga gacggacaaa accacgacct tcttcaccat aggtataccc cggcatgatc
    16321 gcgacttttt cttgctcgat aagcgctttt tgcaacgcgt tgtcgtcaat attcaacgga
    16381 cgtagatcaa gccaggccag ataagtggat tgcgggatct gccagttgag ttcaggaaac
    16441 gcggcgttca ttttatctga gatatacgtc aggttatctt tcagatagac gcgtaaggca
    16501 tccagccacg gtgcaccttg ctgataggcg gcgatatggg cagttaacgc cagtaccgaa
    16561 ggggaagaaa gcccatcacg tcctttcagt gccgataaat aggcatcacg gctgctgcta
    16621 ttttctataa tcccgtaagc accggtcagc gcgggaatat tgaaactttt cgatcccgat
    16681 gtcagcaacg cccagtctcc ccgcgccaca ttactccagg gaatatgcgg ctgctcgccc
    16741 cagaccatat ccatatggat ttcatcggaa ataacccgca catcatgacg ctcgcacagg
    16801 tcagccatga tctccagctc atcgcacgtc cacactttcc cggtaggatt ctgtgggtta
    16861 cacaggagca taattttaca ttctggtttc gccaaaacgg cttccagctt gcccatatcg
    16921 caaaaccagc cgtcagcctg cttctctaaa gcaacgggca ttactgtgcg ctggttacct
    16981 tcaatggcct tgtaaaatgc gtcataggcg ggcgtgtgga tcaccacgcc ttcacctgtt
    17041 tcagaccact gacgaatcag ttctgaaacc atatagatga cagaagggcc atacaccacc
    17101 gtctgcgtat caattgcggt gtaatgctgg gtggaaaacc agtgggcaat agccgcgaga
    17161 aactcatcgt ttttccagcg gctgtagcca aatacgccgt gcatcaggcg ctgattcagc
    17221 gcctcgataa tgcagggggc agtggcaaaa tccatgtctg aaatcgtgaa cggtaacagg
    17281 tcagcagtgc cgaaacggtc agcgacataa tcccactgtg tacaccatgt gccatgacga
    17341 tccacgacct ttgaaaaatc gaacatatct tatccttatg cctggacagt atgcatcaga
    17401 ccggccattt catcttttac tgactgaact tgtggcccga taacaacctg caggttatgt
    17461 tgattaagtt gtactacgcc aattgcccga ttgtccttca gtgcctgcac attaacaagc
    17521 gacatatctt ccacagacaa acgcagacgg gtaatgcagt tatcgaggct gacaatattg
    17581 tcggcaccgc ctaatgcttc gaggattgca ggaacgttgt aacctgattt acccggcgca
    17641 ccggcaacgg ctttttcgat tgagctggca acttcgctat cgcgccccgg ggttttcaga
    17701 ttgaagcggg tgatagcgaa acggaagatg acgtagtaaa cgacaaacca gattgccgcc
    17761 accactggca ccatgtacca cttggttgac agaccatgca aaataccgaa caccacgaag
    17821 tcgatgatat tgccgtcggt attaccgatg gtgacgccga gcacagacat gacggtgaag
    17881 ccgaggccgg ttaacagcgc gtggatgaca tacagaactg gcgctacgaa caggaacagg
    17941 aattccagcg gttcggtagt gccgccaacg acgcaggcga tcaggccaga aatcagcaga
    18001 cctttaattt tatggcgatt ttccgggcgc gcgcagtgat acatagctaa cgctgcacct
    18061 ggtagaccgc cgagaaacgc aggcatttta ccttgcgaaa ggaaacgcgt gacgctttca
    18121 gaaaaaccgt gagtggtcgg gcaactcaat tgcgcctgga agatggtcag tgcgccgctg
    18181 acggtttgac cgcagacctc ctgcgtgccg cctgcgtcgg taaagcgaat taatgccacc
    18241 agaatgtgat gcagaccaaa cggcaacagc agacgttcac cggtaccaaa cagcatcggt
    18301 ccgaaatcac ccgcgctgtt tatcatatgg cccaagccgc taatacccat ggcgaaaatc
    18361 ggccagacta atggaatcac caggccgaca aggcccatca ccagcgagga gataattggt
    18421 acgaagcgcg taccgccgaa gaatgccagc gcatccggca ggcggatatt atggaaacgc
    18481 tcatgcagca tccagacgat aataccggcg atcaccgcac cgaggatccc ggtatcgatc
    18541 gactggatcc caagaatgct ctggatgtta ttggctttca gaacggcggc atctgtggtt
    18601 ggcagaatgc ctttattggt caaccagaag tttaccgcga ggttcattac cgcataaccg
    18661 acgaagccag cgaatgccgc tacgccttta ttttcgcgtg ccaggcccag cgggatggcg
    18721 atacagaaca tgacaggcag gaaactaaaa gcaaacgagc caatcttact catccaggta
    18781 aagatagcct gcaacacggg gttgcccagg accgggatca gggttatgac atcatggctg
    18841 ctaagagaac taccaatgcc gagcataatg ccgcagaacg acaataatgc cacgggtaac
    18901 atgaaggttt tgcctaactg ctggaagaac tcccacagcg tgactttcgg tgctgttttc
    18961 gccgtcataa aacgactcct cgtagagaac aatctggatt cagtaaattg cgcgagaaga
    19021 taaaacgttt tatcaaattt tagtgagtca taaatcacat tacgcaacga taatagcggg
    19081 tataagataa ataaaaggta aaacgtttta tctgtcacat aatcagggag taggtcatct
    19141 gcatggctac cgccaaaaaa ataaccattc atgatgttgc gctggctgcg ggcgtgtcgg
    19201 taagtaccgt ttcgctggtg cttaatggca aagggcgaat ctctaccgcc acaggagaac
    19261 gcgttaacgc cgccattgaa gagctgggat ttgtgcgcaa tcgacaggcg tcggcgctgc
    19321 gcggcgggca aagcggcgtc attggtttga tcgtccgtga tttatctgcg ccgttttacg
    19381 ccgaattgac ggccggattg acggaagctc tggaagcaca gggacggatg gtttttttgc
    19441 ttcacggcgg taaagacggc gagcagctgg cacagcggtt ttcactgtta ctgaatcagg
    19501 gtgtcgatgg tgtggtaatt gccggggctg caggaagcag cgatgacctg cggcggatgg
    19561 cagaagaaaa agctatcccg gtgattttcg cttcccgtgc cagttatctt gatgatgttg
    19621 atacggttcg cccggacaac atgcaggctg cacagttgtt gacggagcat ctcattcgca
    19681 atgggcatca gcggatcgcc tggctgggag ggcaaagttc ctcattaacc cgtgcagaac
    19741 gggtgggggg ctattgtgca actctactaa aatttggcct gccgtttcac agcgattggg
    19801 tgttggagtg cacttccagc cagaagcaag ccgcggaagc tatcacggcg cttttacgtc
    19861 ataacccgac catcagtgcc gtggtttgct ataacgaaac tattgcgatg ggggcatggt
    19921 ttggtttgct gaaagcaggg cggcaaagcg gggaaagcgg agtcgatcgt tactttgagc
    19981 aacaggtttc gctggcggca tttaccgatg cgacaccaac cacacttgat gatatacctg
    20041 ttacctgggc cagcacgcca gcgcgggaac ttggtatcac acttgcggat cgcatgatgc
    20101 aaaaaatcac ccatgaagag acgcattcac gcaatcttat tattcccgcc cggctcattg
    20161 cggcgaaata atcctctctt tatctgctat acttggtagt gtcctttcct caaggttaat
    20221 gataaaaaat gtggcacaca ggcggtgcct tttattttta ctgaagttca caggaggttt
    20281 atgtgtttaa ttctgacaac ctgagactcg acggaaaatg cgccatcatc acaggtgcgg
    20341 gtgcaggtat tggtaaagaa atcgccatta cattcgcgac agctggcgca tctgtggtgg
    20401 tcagtgatat taacgccgac gcagctaacc atgttgtaga cgaaattcaa caactggatg
    20461 gtcaggcatt tgcctggcgt tgtgatatta cttccgaaca ggaactctct gcactggcag
    20521 actttgccgt cagtaatctg ggtaaagttg atatcctggt taacaacgcc ggtggcggtg
    20581 gtcctaaacc gtctgatatg ccaatggcag attttcgccg tgcttatgaa ctgaatgtgt
    20641 tttctttttt ccatctgtca caacttgttg cgccagaaat ggaaaaaaat ggaggtggcg
    20701 ttattctgac catcacttct atggcggcag aaaataaaaa tataaacatg acttcctatg
    20761 catcatctaa agctgcggcc agtcatctgg tcagaaatat ggcatttgac ctgggtgaaa
    20821 aaaatattcg ggtgaatagc attgcgccgg gggcaatatt aaccgatgcc ctgaaatccg
    20881 ttattacacc agaaa
//
LOCUS       DAOFMX010000071        20737 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.071, whole genome shotgun sequence.
ACCESSION   DAOFMX010000071 DAOFMX010000000
VERSION     DAOFMX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.071"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="QBA20_002990"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="QBA20_002990"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDQ6293438.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="QBA20_002991"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="QBA20_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDQ6293439.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="QBA20_002992"
     CDS             complement(2649..3200)
                     /locus_tag="QBA20_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDQ6293440.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="QBA20_002993"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="QBA20_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDQ6293441.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="QBA20_002994"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="QBA20_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDQ6293442.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="QBA20_002995"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="QBA20_002995"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDQ6293443.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="QBA20_002996"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="QBA20_002996"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDQ6293444.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="QBA20_002997"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="QBA20_002997"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDQ6293445.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="QBA20_002998"
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="QBA20_002998"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDQ6293446.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNDQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="QBA20_002999"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="QBA20_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDQ6293447.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="QBA20_003000"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="QBA20_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDQ6293448.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="QBA20_003001"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="QBA20_003001"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDQ6293449.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="QBA20_003002"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="QBA20_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDQ6293450.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="QBA20_003003"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="QBA20_003003"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDQ6293451.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="QBA20_003004"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="QBA20_003004"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDQ6293452.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="QBA20_003005"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="QBA20_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDQ6293453.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="QBA20_003006"
     CDS             complement(18174..18569)
                     /locus_tag="QBA20_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDQ6293454.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="QBA20_003007"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="QBA20_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ6293455.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="QBA20_003008"
     CDS             19485..20078
                     /locus_tag="QBA20_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDQ6293456.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /gene="tabA"
                     /locus_tag="QBA20_003009"
     CDS             20223..20675
                     /gene="tabA"
                     /locus_tag="QBA20_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDQ6293457.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4931 a         5211 c         5503 g         5092 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcacgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtaaag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgctgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 cgttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaaa ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gatgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgccac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgc atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tctaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tagagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg gggggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gatcgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gccatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgactttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acgcaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctctgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg ctcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc taggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg tgcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac acctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttatcggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacatgttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagt ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcagggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacatta ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag catgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca
    20701 ggtttacggc gagtttgtga aaagagcgtt ttttgat
//
LOCUS       DAOFMX010000072        20712 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.072, whole genome shotgun sequence.
ACCESSION   DAOFMX010000072 DAOFMX010000000
VERSION     DAOFMX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.072"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="QBA20_003010"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="QBA20_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDQ6293458.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="QBA20_003011"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="QBA20_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDQ6293459.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="QBA20_003012"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="QBA20_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDQ6293460.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="QBA20_003013"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="QBA20_003013"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDQ6293461.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="QBA20_003014"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="QBA20_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDQ6293462.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="QBA20_003015"
     CDS             complement(8217..9110)
                     /locus_tag="QBA20_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDQ6293463.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="QBA20_003016"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="QBA20_003016"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDQ6293464.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="QBA20_003017"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="QBA20_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDQ6293465.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="QBA20_003018"
     CDS             complement(10241..11473)
                     /locus_tag="QBA20_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDQ6293466.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="QBA20_003019"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="QBA20_003019"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDQ6293467.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="QBA20_003020"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="QBA20_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDQ6293468.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="QBA20_003021"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="QBA20_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="QBA20_003022"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="QBA20_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDQ6293469.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="QBA20_003023"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="QBA20_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDQ6293470.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="QBA20_003024"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="QBA20_003024"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDQ6293471.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="QBA20_003025"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="QBA20_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDQ6293472.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4982 a         5726 c         5032 g         4972 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aagttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaatgt gc
//
LOCUS       DAOFMX010000073        20699 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.073, whole genome shotgun sequence.
ACCESSION   DAOFMX010000073 DAOFMX010000000
VERSION     DAOFMX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.073"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            81..350
                     /locus_tag="QBA20_003026"
     CDS             81..350
                     /locus_tag="QBA20_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDQ6293473.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="QBA20_003027"
     CDS             344..709
                     /locus_tag="QBA20_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDQ6293474.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="QBA20_003028"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="QBA20_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDQ6293475.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="QBA20_003029"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="QBA20_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDQ6293476.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="QBA20_003030"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="QBA20_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDQ6293477.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="QBA20_003031"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="QBA20_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDQ6293478.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="QBA20_003032"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="QBA20_003032"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDQ6293479.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVSNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="QBA20_003033"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="QBA20_003033"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDQ6293480.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="QBA20_003034"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="QBA20_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="QBA20_003035"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="QBA20_003035"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDQ6293481.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="QBA20_003036"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="QBA20_003036"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDQ6293482.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14857..16455
                     /gene="rtcR"
                     /locus_tag="QBA20_003037"
     CDS             14857..16455
                     /gene="rtcR"
                     /locus_tag="QBA20_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDQ6293483.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16437..17195)
                     /locus_tag="QBA20_003038"
     CDS             complement(16437..17195)
                     /locus_tag="QBA20_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDQ6293484.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17212..18042)
                     /gene="glpG"
                     /locus_tag="QBA20_003039"
     CDS             complement(17212..18042)
                     /gene="glpG"
                     /locus_tag="QBA20_003039"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDQ6293485.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18087..18413)
                     /gene="glpE"
                     /locus_tag="QBA20_003040"
     CDS             complement(18087..18413)
                     /gene="glpE"
                     /locus_tag="QBA20_003040"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDQ6293486.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18603..20108
                     /gene="glpD"
                     /locus_tag="QBA20_003041"
     CDS             18603..20108
                     /gene="glpD"
                     /locus_tag="QBA20_003041"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDQ6293487.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20377..20607)
                     /locus_tag="QBA20_003042"
                     /pseudo
     CDS             complement(20377..20607)
                     /locus_tag="QBA20_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4891 a         5693 c         5217 g         4898 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgagac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgaac gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttttt aatttattgt
    14761 ttctaaaaag attatatctt tatgtccgta aacggagatt tcccgcaaag ccgatttacc
    14821 gataatgaaa tatcgccttt tataaggata tctaagatgc gtaaaacagt ggcttttggc
    14881 tttgtcggta ccgtgctgga ttatgccgga cgcggcagtc agcgctggtc aaaatggcgt
    14941 ccgtcactct gtatatacca gcaagaatcg ttggtcatcg atcgactgga attattgcac
    15001 gatgcccgct cgcgctcgct atttgaaacg cttaaacgcg atatcgccag cgtttcgcca
    15061 gaaacagaag tggtgggcgt tgagattgaa ctgcataacc cgtgggattt cgaagaggtt
    15121 tacgcctgcc tgcatgattt cgcccgtggt tacgagtttc agccagaaaa agaagactat
    15181 ttaattcaca tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcggaa
    15241 gcgcgttatc tacccgcccg gttgatacaa tcttcaactc cgcgcaaaaa agaacagccg
    15301 cgcggcccag gtgaagtgac gattatcgat ctcgatttaa gccgttataa cgccatcgcc
    15361 agccgctttg ccgaggaacg gcagcaaacg ctcgatttcc tcaaatccgg tattgccacg
    15421 cgtaaccccc acttcaaccg catgattgag cagatcgaaa aagtggcgat taaatcccgc
    15481 gcgccgattc tgcttaacgg cccgaccggc gcaggtaagt catttctggc gcgacgcatc
    15541 ttcgagttaa aacaggcacg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc
    15601 accctgcgcg gcgataccgc gatgtcgacg ctgtttggtc atgtgaaagg cgcgtttacc
    15661 ggggcgcggg aatctcgtga aggattatta cgcagcgcca acggcggaat gttgtttctt
    15721 gatgagattg gcgaactggg cgcagacgaa caggcaatgc tgctgaaagc tattgaagag
    15781 aaaacctttt acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc
    15841 ggaacggtgc gcgatttgcg ccagctggtt gccgaaggca aattccgcga agatctgtac
    15901 gcgcggatca atctctggac cttcaccctg ccgggtctac gccagcgcca ggaagatatt
    15961 gaaccgaacc tggattatga agtggagcgc cacgccacac tcactggtga cagcgtgcgt
    16021 tttaacaccg aagcgcggcg tgcctggctg gcctttgcaa catctcccca ggcaacatgg
    16081 cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc
    16141 ggacgcatca ctctggacgt ggtggaagat gagataaacc gtctgcgcta taactggcag
    16201 gagagccgcc cctccgcgct tacggcgttg ctgggcgcag aggcagaaaa catcgatctc
    16261 ttcgaccgta tgcaactgga acacgttatc gctatctgcc gccaggcaaa gtcgctttct
    16321 gctgccggac gacagctttt tgatgtttcg cgccagggca aagccagcgt caatgacgcg
    16381 gatcggctac gcaaatacct ggcgcgtttt ggtctgacgt gggaagccgt gcaggatcag
    16441 cacagctcca gttgaatatg gtggtccgtc agcacctgca tcacgcttac tggcggcggg
    16501 gcgtcggtgt agacggcatc aaccatgctg atgctcccca tattgaccat cgcattacgg
    16561 ccaaattttg aatggtcaac caccagcata acgtggcgcg agttctcaat aatggcgcgc
    16621 ttggtacgaa cttcgtggta atcgaactcc agcagcgagc cgtcgctgtc gatgccgctg
    16681 atccccagaa tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca
    16741 atgatcccgc catcgcggct acgtaattcg ccaccggcga gaataatgcg gaaatcttct
    16801 tttaccatca acgtgttagc aacgttgaga ttgttggtga caatgcgcaa attgctgtga
    16861 ttgagcagcg cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagcgtcgag
    16921 ccattgggga tttgctccgc cactttgcgg gcgatgcgct ctttttcttc ggtctgggtg
    16981 gctttacgat cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc gccgccatga
    17041 tggcgcagga tcaggttttg ctctgccagc tcgttgaggt cgcggcgaat agtctgcggg
    17101 ctgacggaga agtgctccac cagttcctcg gtactgacat aaccctgctg tttaaccagt
    17161 tcgataatac cgttgtgacg ttgtgtttgt ttcatttata aatccctgga attattttcg
    17221 ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa cccacggcta atccggcgat
    17281 gtgtgctccg ttcgccatcg acatcccaaa caaatcaaac catccggcga caatccagat
    17341 cagcgcaaag ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgttcgcc
    17401 acgtagccag acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg
    17461 cccgctgaat ttttgctgca tatagccgct taacagggcg ctgataagcg taatgacaat
    17521 tagcttaccg ctaccgaggc gtttttccac cgcgccgccg agataccacc accaaagcag
    17581 gttaaagagg atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa
    17641 ctcaaatttc agtgttggat cgaatggcca ggccagccat aacatcactt cctgatcgcc
    17701 gagaatttgc atggcaataa acaccaccac gcaggcgatc atcatcaccc aggtcaccgg
    17761 acctgcgcgt tcacgcaagg cagcaaagaa aggataacgg cgataatgca ggccactgcc
    17821 ggtatggcct gccagccagc tggccgccag ataacgcgga tctgccgggt tttcgagaaa
    17881 acgcgccagc tccgcccgca cgcgctcggc ctgggactca tccgccagcc agacatcgct
    17941 ttggttatgt tgttgaatcg tgaggataac accctgcgtc gccatgtaat caacaaacgc
    18001 ctgcgccacg cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc
    18061 cacacaaaag gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactgt
    18121 cgctgccagg cttcaaagcc gccgtcaatg ctatagacca catcgtagcc ctgttgcagc
    18181 agatactgcg ccgccccttt gctgctattg ccgtgataac acatcaccat caccggagtg
    18241 tcaaagtcgt tatcacgcat aaacgcgccc agcgtgtcgt tggttaaatg gaaagcctgc
    18301 accgcatgcc ccatagcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt
    18361 tcctgcaact tctggtgcgc gtcggcaacg ttaatacatt cgaactgatc catgcgtctc
    18421 tctttcttta caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa
    18481 acgccataat gttatacata tcactctaaa atgttttttc aatgttaccc aaagcgcgat
    18541 tctttgctaa tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca
    18601 gcatggaaac caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg
    18661 cagacgccgc tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg
    18721 cgacctcttc cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg
    18781 aattccgcct agtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggctccgc
    18841 atatcgcctt cccgatgcgt tttcgcctgc cacatcgtcc gcatctgcgc ccggcgtgga
    18901 tgattcgcat tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat
    18961 caactggttt gcgttttggc gcaaattcag tgttaaaacc ggaaattaag cgcggattcg
    19021 aatattctga ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg
    19081 tgcgtaaagg cggcgaagtg cttactcgga ctcgcgccac ctctgctcgc cgcgaaaacg
    19141 gcctgtggat tgtggaagcg gaagatatcg ataccggcaa aaaatatagc tggcaagcgc
    19201 gcggcctggt taacgccacc ggcccgtggg tgaaacagtt cttcgacgag gggatgcatc
    19261 tgccttcgcc ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgcgtgc
    19321 atacccaaaa gcaggcctac attctgcaaa acgaagataa acgcattgtg ttcgtgatcc
    19381 cgtggatgga cgagttttcc atcatcggca ccaccgatgt ggaatacaaa ggtgatccga
    19441 aagcagtgaa gattgaagag agtgaaatca attacctgct gaaagtgtat aacgcgcact
    19501 ttaaaaagca gctaagccgt gacgatatcg tctggaccta ctccggtgtg cgtccgctgt
    19561 gtgatgatga gtccgactcg ccgcaagcca ttacccgtga ttacaccctt gatatccatg
    19621 atgaaaacgg caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgaa
    19681 aactggcaga acatgcgctg gaaaaactga cgccgtatta tcagggtatt ggcccggcgt
    19741 ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattacgccg
    19801 ctcgcctgcg ccgccgttac ccgttcctga ctgaatcgct ggcacgtcac tacacgcgca
    19861 cttacggcag caacagcgag ctgctgctcg gcaatgcggg aacggtaagc gatctcgggg
    19921 aagatttcgg tcatgagttc tacgaagcgg aactgaaata cctggtggat cacgaatggg
    19981 tccgccgcac cgacgatgcc ctgtggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg
    20041 atcaacaatc tcgtgtgagt cagtggctgg tggaatatac gcagcagaag ttgtcgctgg
    20101 cgtcgtaaat taacgtaagg tgatcaggtc agatttcaat ctggcctgag actgatgaca
    20161 aacacaaaac tgcctgatgc gctacgctta tcaggcctac gtggtttatg caatatattg
    20221 aatttgcatg gtcttgtagg ccagataaga cgttcacgtc gcatccggca tgaacaaagc
    20281 gcactttgtc aaaaatctaa cctacttttt aattcaggga attaccgcaa agcccacgcc
    20341 atcataccca acgtaacaaa actcaggcac gttccccact cgccccgaga aaaatagcat
    20401 taatgcgccc agcgtcagca taaaaatatt gagcagaagg taaattgttg gcgtaaagag
    20461 taaattgcaa acctcaggaa tagcggcaag cccagccccc agcatggcaa taaccgctgc
    20521 aaaaaattgc gtcagtattg ggatgccgaa aaaagtcatt accagcgagg tcaaaatcca
    20581 ttttctattt tgcattattc tttccattct ttctgaatgg cgaaattata ctccccgagt
    20641 ccccttgccc cttctggaca cttttccgaa atgatggcga aaaaaacggg accctttgg
//
LOCUS       DAOFMX010000074        20679 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.074, whole genome shotgun sequence.
ACCESSION   DAOFMX010000074 DAOFMX010000000
VERSION     DAOFMX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.074"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..579
                     /locus_tag="QBA20_003043"
     CDS             <1..579
                     /locus_tag="QBA20_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDQ6293488.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="QBA20_003044"
     CDS             597..1235
                     /locus_tag="QBA20_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ6293489.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQVNSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="QBA20_003045"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="QBA20_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDQ6293490.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3200)
                     /gene="ycjG"
                     /locus_tag="QBA20_003046"
     CDS             complement(2229..3200)
                     /gene="ycjG"
                     /locus_tag="QBA20_003046"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDQ6293491.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALALATEARAQGFSLMLG
                     CMLCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3319..3825
                     /gene="tpx"
                     /locus_tag="QBA20_003047"
     CDS             3319..3825
                     /gene="tpx"
                     /locus_tag="QBA20_003047"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDQ6293492.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3869..5410)
                     /gene="tyrR"
                     /locus_tag="QBA20_003048"
     CDS             complement(3869..5410)
                     /gene="tyrR"
                     /locus_tag="QBA20_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDQ6293493.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5558..6619)
                     /locus_tag="QBA20_003049"
     CDS             complement(5558..6619)
                     /locus_tag="QBA20_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDQ6293494.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6616..8013)
                     /locus_tag="QBA20_003050"
     CDS             complement(6616..8013)
                     /locus_tag="QBA20_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDQ6293495.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8169..9167
                     /locus_tag="QBA20_003051"
     CDS             8169..9167
                     /locus_tag="QBA20_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDQ6293496.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9278..10150)
                     /gene="ompG"
                     /locus_tag="QBA20_003052"
                     /pseudo
     CDS             complement(9278..10150)
                     /gene="ompG"
                     /locus_tag="QBA20_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10223..11304)
                     /locus_tag="QBA20_003053"
                     /pseudo
     CDS             complement(10223..11304)
                     /locus_tag="QBA20_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11318..11977)
                     /gene="pgmB"
                     /locus_tag="QBA20_003054"
     CDS             complement(11318..11977)
                     /gene="pgmB"
                     /locus_tag="QBA20_003054"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDQ6293497.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11974..14199)
                     /gene="ycjT"
                     /locus_tag="QBA20_003055"
     CDS             complement(11974..14199)
                     /gene="ycjT"
                     /locus_tag="QBA20_003055"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDQ6293498.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLRPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGEEETPEFAAINIRTGLRQK
                     VASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLE
                     IHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDAFIHRAEMFLKELRL
                     PEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEVNEMQILKQADVVML
                     NYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEI
                     DLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALLEQWQQLSFPLFW
                     QGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDFILPFNGTATTHQED
                     E"
     gene            complement(14196..14819)
                     /locus_tag="QBA20_003056"
     CDS             complement(14196..14819)
                     /locus_tag="QBA20_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDQ6293499.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14816..15238)
                     /locus_tag="QBA20_003057"
     CDS             complement(14816..15238)
                     /locus_tag="QBA20_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDQ6293500.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15260..15971)
                     /locus_tag="QBA20_003058"
                     /pseudo
     CDS             complement(15260..15971)
                     /locus_tag="QBA20_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(15989..17041)
                     /locus_tag="QBA20_003059"
     CDS             complement(15989..17041)
                     /locus_tag="QBA20_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDQ6293501.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17072..17914)
                     /locus_tag="QBA20_003060"
     CDS             complement(17072..17914)
                     /locus_tag="QBA20_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDQ6293502.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(17901..18782)
                     /locus_tag="QBA20_003061"
     CDS             complement(17901..18782)
                     /locus_tag="QBA20_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDQ6293503.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18803..20086)
                     /locus_tag="QBA20_003062"
                     /pseudo
     CDS             complement(18803..20086)
                     /locus_tag="QBA20_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20100..>20679)
                     /gene="ycjM"
                     /locus_tag="QBA20_003063"
     CDS             complement(20100..>20679)
                     /gene="ycjM"
                     /locus_tag="QBA20_003063"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDQ6293504.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5052 a         5628 c         5026 g         4973 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggtaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca gcgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgccagc gcttccgtca gaccaccggt tttatcgagc
     2461 ttaatgttaa ccatctcata gcgccctttc agcgccttca gattgctacg agtatgacaa
     2521 ctttcatcag cacaaatcgg caacggatga ataaaattct ccagcgccgc atcgtcctgc
     2581 gcaggaagcg gttgttcaag catcgcaacg cctaaatccg ccaatagctg gcaacgcgcc
     2641 gccaaccctt ctgcacgcca ggattcattt gcatcaacga tcagcgtggc atcgggcaca
     2701 gcggtgcgaa ttgccaccat ccgctcactg ataagatggt tatccagctt cactttcagt
     2761 aatttcgcgc ctgcctgcca gagtgttgat gcactattgg ccatctgatc aggcgtaccg
     2821 atgacaaccg tctgtgcagt gataactgtc tcgggaagcg ttatgccgat caaatcagcc
     2881 agcgattgct gctgttttcg cgccgcgaga tcccacaatg cacaatccag cgcattacgt
     2941 gctgcgccgg cagggagaat tttttgcaac tcctcccgtg tcagcccttt ctctaattgc
     3001 ggcacgacgc tcataatttg cgccattacc gaggcatcac tttctccata acgcggatac
     3061 ggcgtgcatt cgccggtgcc tttaataccc tcttcttcca gttcaaccac caccacgcgc
     3121 gcttcactgc gacttccccg ggcaatcaca aacggggtat gtaagggcca ggcttcctca
     3181 aataccttaa cgattctcat ctttactcct tgttgtcgcg gtggtctgcg gattaagcgc
     3241 aaatagcgtt tgctgtgtta ttgacagtta gcataaacta ggtgtgacgt taactatatg
     3301 taaacaggaa agataatcat gtcacaaacc gttcatttcc agggcaaccc ggttacagtc
     3361 gccaattcca tcccgcaggc gggtagcaaa gcgcagactt ttactctcgt ggcaaaagat
     3421 ctgtctgacg tcaccctcgg tcagtttgcg ggtaaacgca aagtgctgaa cattttcccg
     3481 agtattgata ccggtgtttg cgccgcatca gtacgtaagt ttaaccaact ggcaactgag
     3541 atcgacaaca ccgttgtgct gtgtatctct gccgatctgc cgttcgccca gtctcgtttc
     3601 tgcggcgcag aaggtctgaa caacgttatc accctctcca ctttccgtaa cgctgaattt
     3661 ctgcaagcct acggtgtggc aattgctgat ggcccactga aaggtctggc agcgcgtgcc
     3721 gttgtggtta ttgacgaaaa tgacaatgtg attttcagcc agctggtgga tgaaatcacc
     3781 accgagccgg attacgaagc agctctggct gtactgaaag cataagttaa tatgcctgat
     3841 ggtgttgcac catcaggcat attcgcgctt actcttcgtt cttcttctga ctcagaccat
     3901 attcccgcaa cttattggca atcgcggtat gtgaaacgcc gagacgtttt gccagtttgc
     3961 gcgtgctggg ataattgcga taaagctggg ttaataccga gcgttcaaaa cggctggtga
     4021 tttcgtccag cgaaccttcc atcgcatctt cgcccacggc taccgttgcg gcgtcataat
     4081 ccggcaacaa aatatcctgt ggacgcagct cataaccgtc cagttgtgtc agtgcgcgat
     4141 agatagcgtt ctttaactgc cgcacatttc ccggccacgc ataacgtgta agtacagtat
     4201 tcaggtcagc ggccagtttc ggacgcggca cgccctgctc gtcggcaaag cgggcgacga
     4261 acagctcagt taacggcata atgtcctgcg gacagtcacg tagcggcggc agattgagcg
     4321 tcaacacgtt cagacgataa tagagatctt cacggaacac gcctttttgc accagttcga
     4381 ccagattctt ctgcgtagcg caaatcaccc gcacatcgac atgcacctca tggtcttcgc
     4441 caacccgacg gaaagtgcca tcattaagaa aacgcagtaa tttcgcctgc atccgtggtg
     4501 acatttcccc tatttcatcc aacagcaccg aaccaccgtt cgcctgctca aagaaccctt
     4561 tcttcccttc cggagcatga ccaaacagtt cactctcgac cgcatcttct ggtatagacg
     4621 cgcaattcag cgccaggtaa ggtttgcccg ctctggggct tgcctgatgg caggcgtagg
     4681 caaaaagatc tttacctgta cctgtgtcac ccgtaatcag cagcggcgcg cttagcatcg
     4741 ccagtttctg cgcctgttcg acaacgtgct tcattttcgg gctgacggcg acaatttggc
     4801 tgaaggcgct gacgtcctgg gcggcgacat tttgcaactg gcggcccata cgaatcgttg
     4861 atcgcaacat caccaccgca ccggtcagga cgtgttgatc attttcatcc tgaagataaa
     4921 caggcgtaat ctccatcagg aaattctgcc cattaataac gacatgctcg ttatgcgaat
     4981 cttgcggttc gctttccagc caacgtaaaa aattaaagcc gttaatcaat tgtgcggcgg
     5041 tatggttgcg caggcgatcc aatttttgcc caaaaagctg acagctcgcc gggttcgcca
     5101 tatccacttt gcttttcata tcgacagaga gcacaggttc aggcaacgcc tccagtaacg
     5161 cgctcaacgc cagatgctca cgttcggaag gcatccacgg gacagtacgc acatcggtaa
     5221 cacctgcaat acggcgtatt tcggccatca gactgctgaa actctcaaac tccagttcag
     5281 caaaattgag gtagattcgc ccaatgggat caatctcaat accgcgtaaa tcaatgcctc
     5341 ttagcacgag tagatcgagt aattcgcggg tcagaccgag tcggtcttca caaaagactt
     5401 ccagacgcat gggaaccttc acctgaaaaa agaacaatta atatgatgat atgacactat
     5461 ttggatagca ggaaggtttc tgtcaacaat cattgacaca aagacggaaa aacgggcact
     5521 tcggcgtaaa gatatccggt cagcacccga tattgcatta tttttcgctg ggcgttttgc
     5581 ctttttgcag agtttctttc acctgaccga taagctgacg acggaaatcc ccaaggcgag
     5641 gtttgtcatc gtcaatccac ggcagcgggc ggcaaagctc catagcttta atcccgagtc
     5701 gtaccgtcag aagtccagca ccaattccct gagctgcgcg ggtagacaaa cgagcagcga
     5761 gatcttgcga catccagtcc atccccactt cgcgcaccag ttcgctggct ccggcaaaag
     5821 cgatattcag caataccagc ttaaacagac gcaaacggct gtaatacccc agttcaatgc
     5881 catacagcgt ggcaatgcga ttaattaaac gcagattgcg ccaggcgata aacgccatat
     5941 cgaccaacgc cagcgggctg accgcaatca tcaacgttga ttccgccgcc gaatggctga
     6001 tttcgcgccg cgcctgggca tctaaaactg gctggaccaa atgcgcatac aggctgacca
     6061 cttcacgatc gttctgcgtt tcatggattg aggcatacca gcgttgcagc gccggatgcg
     6121 actgatcaat acccgcctgc tgcgccagtt tttcgcaaaa tgcgcgacct ttgcccgtgc
     6181 cgtggctatg caataaatca cgcgcttcgt cgcgttcatg ggcgcgctgt cgcaagcgcc
     6241 ataagcgccg ccactctgtt accacagaac ctacgccagc gccgataatc aatgccccag
     6301 cggcacatcc acccagcgct acccagtcct gggtttgcca ggcattcatt gtccactgta
     6361 taccctgccc gacaacgctt gcgccaaaca gagccagccc gcccatcacc attttgcgcc
     6421 acaggctgcg tttcggacgt aacgctgcgt ccattaccgc ttcgacttgc ccctcttctt
     6481 cctgcgcttc gtcgagcgtg gccggggcaa aattttgcgc ctgattttcg tcaaaggtct
     6541 gctgcgccct gaattttgga ttctgttcga cctccagcgg accgtcgaaa tcaatacgtg
     6601 gttttaacgg ttcggtcatc gcaatttatc tcctattaaa aattccagcg cggcatcaag
     6661 acgaatatgc ggcagcggtt tgtcgacatc catcacctgc gggcgaaacg cttcaaactg
     6721 gaacccttgc ttgtcccaga acgcctgacc gggcaaacgc gcaggaactt caccggggta
     6781 aacggtgaga ggcgcaccat cgctgagccg gttaccgcgc aatgccggaa ttttctcgcc
     6841 gttgacgtca ataatgccgc tggtggtcgc ctgcaccgag gccagcccca ggcaatccat
     6901 actaatcctt tcaaacgccg cattttgcca ggcatcctga atcagttgtt gcagtaatga
     6961 aaccatattg gcgtgctgat cgaccgttac atggtccgct ttcatcgcgg caaacagcag
     7021 tttgtcgata accggcgaga ataagcggcg aaacagcgtg cgctgcccgt agtggaaact
     7081 ttgcatcagc tgcgtcagtg ccagacgcat atcattaaat gcctgtggcc cactgttgag
     7141 aggttgcagg caatccacca gcacaatctg gcggtcaaag cgcagaaaat gattcttata
     7201 gaaccccttc accactttct cgcagtaata attaaaccgc tcgcgcagca ttccggcatt
     7261 ggtgtgctta tcggcctgcg ccagtttgga ctcgtcccag gcatcgacat ccggccacgg
     7321 gaagaattgc agcgcgggcg cacctgccat atctcccggc aagacaaagc gcccaggctg
     7381 aataaagtgc agcccctgct gtttacagtg gtggagataa tcggtccaca cggcggcaat
     7441 gtctgccagc cggttttcgt cggcaggcgc tagcgggtca agtccttcgc acatcattcg
     7501 ccatttcgcc gaccattcgc cgcgctgacc attgagtaag cccgtcatct ggcgcgacca
     7561 gcttaagtaa tcctgcgcca gcatcggcag gtcgagcaac cattcgccgg gataatccac
     7621 aatttccaga tacagcgtgg aggtatcctt aaagtggcgc agcagcgaat cgttcgattt
     7681 aaaacgtaac gccaggcgaa tttcactgac gccgcgcgta ggcgtcggcc aggcgggagg
     7741 atcaccatat aactgcgcca gtccttcatc ataggtgaag cgcggaatgc caaagtcacg
     7801 ctggggaatg cgttttacgc ccagcaagcg ctcttcacgc accgcgctta acagcggcaa
     7861 acgtgctccg gcatgaatat tgagcaactg attaaccatc gcggtgataa acgctgtttt
     7921 gccgctgcgg ctaagtccgg ttacagcgag gcgcagatgt ctgtcgacac cccgattcac
     7981 cagcgcattg agttcatttt taagtcgctt cattgctgtc ctgtgctgct ctgaagacca
     8041 gaaaaaaaat cattcgtaag gatatgaatg tgggggccag gccattgatt atcaactgcc
     8101 cgtgttgtca tcgacgggcg aactccataa gatgtgcagc attcacgttc ggacggagtt
     8161 cctgacctat gtcccctact atttatgata ttgccagggt tgcaggcgta tcaaaatcca
     8221 ccgtatcacg cgtgctgaat aagcaaacta atatctcccc ggaagcgcgc gaaaaagtgt
     8281 tacgggccat tgaagaatta cagtatcagc caaacaagct ggcccgcgcg ctgacctctt
     8341 cgggttttga tgccattatg gtgatttcta cccgttcgac caaaactacg gcgggtaatc
     8401 cgtttttctc tgaagttttg catgccatca ccgccaaagc ggaagaagaa ggtttcgacg
     8461 tgatattaca aacgtcgcac aatccggcag aagatctgca aaaatgcgaa agcaaaatta
     8521 aacagaaaat gattaaaggc attattatgc tcagttcgcc agcggatgag tcattttttg
     8581 cccaactcga taaatacgat attccggtgg tggtaattgg caaagttgaa ggtcaatatg
     8641 cccatgttta ttctgtcgat accgataatt ttggtgacag cattgcgttg accgatgcat
     8701 taattgaaag tgggcatcaa aatattgcct gcctgcatgc tccgcttgat gtccatgttt
     8761 cggttgatcg cgttaatggc tataagcaaa gtctgtccgc gcataatatt gcagtgcgtg
     8821 ataaatggat tgttgacggc ggttataccc atgaaacagc actgcaagca gcacggcaat
     8881 tattaagtca gtcaccgttg cctgaggcag tgtttgccac tgacagcctg aaattaatga
     8941 gcatttatcg tgcggcggca gagaaaaata ttgctattcc gcagcagtta gcggtggtgg
     9001 gttatagtaa tgaaacgctg tcgtttattt taacgcctgc accaggcggc atcgatgttc
     9061 cgacgcagga gttaggtcaa caaagctgcg agttattatt ccgcttaatt tcgggaaaac
     9121 cgtcaccaca gaatattacc gttgccacgc atatgacgct gaaataaata ccagctacct
     9181 tgcctgaatg ccggatgcga tgctggcgca tcttatccgg cctacattta tagatcggat
     9241 aaagtactcg cgccgcatcc ggcaatttag cccattatca gaacgagtaa tttacgccga
     9301 cacctgcata atggaattta tcgctgtcgc cttcgtcgtg atcctgccac tcaaacgcgt
     9361 attccagcga aacggaaagt ccgttctgga aatcataacc gtaaaataaa cccacacggt
     9421 taaaatcatg gccttcacgt tcaatatcat cctgccagtc ccagttactc tagcgatcca
     9481 gcccaatgcg cgtatacggc gtcaccgagt ggttgccgag cgtcagcggc aaataggcgc
     9541 gaatttcttg tgtggaaaac tcaccgttat tgcggctgtc gtccatattg aagccgcgct
     9601 cgagataata gttcactcgc aaagcaaccg tttcgttgaa ggtatattgc agacctgttt
     9661 ccgtttcgac acgggtatca gcgtaaccgg tcgtgttcaa atcgttggca aatttataca
     9721 tcgacaacca accgttgaaa cgtaaatcat cagtcagttt cacatcccag tctggcgcga
     9781 ttttccagcg ctgcatattc gccgtgtctt tgcccggttc atcaacgtag tgataaccat
     9841 aattacggaa accgccggtc aggccgaaac tgaaatcatc gctttcgagg aactgataat
     9901 gcacctccag ctccgggcga tcaaacctcg ttccacgttt acccgcgcta taatctaccg
     9961 gcccttcctg ataataggcc agagcaattc tccacggccc gttggcggca ttaaaataga
    10021 ctgaaggctc cgccagccca tccatatctt cgccataacc ctcgacgttt tctatttcgt
    10081 acatcgcacc gatattaaag tgccagtcgc tcttttcctc ggcctgtgcg caggccattc
    10141 ccgcacacat caccagtgcg gtacagggta ataacttttt ttgttccttg tattatcctg
    10201 ggggaatgcc ttccccctgt atttagcgaa ttgctatttc cgtttctgca tcaaagaaat
    10261 gacatttcgt catatcaaaa tgaatagtga tattttctcc tgcatgataa tcatttaacg
    10321 caccagcacg gaccactaac tcgtgccccc aaccgtggtg tagagcataa attccgcacc
    10381 ggttaattct gccacgctaa ttttggcgga aatgttattt tcctcttgcg catccggatg
    10441 aatatcttcc gggcgtattc ccatcacgat gggcttatgc aaactttcct gtgttttcag
    10501 aaccgctaat ttctcttcga gaatggttaa tttaagcgtt tccgtaacga atttatcgcc
    10561 atcgatcgtg ccgcgaataa aattcatcgc tggtgatcca ataaatccgg caacaaacat
    10621 attcgcgggt tggttataaa cggttttcgg cgcacctact tgctgaacaa tcccgtcttt
    10681 cataatcaca atccgcgccg ccatggtcat cgcttcggtc tgatcgtggg tcacgtagat
    10741 catggtggtg ttcagtttct gatgcagctt gctgatctct gcacgcattt gtacgcgcag
    10801 cttggcatca aggttagaga gcggttcatc cattaaaaac acgcccgctt cgcgcacaat
    10861 cgcccgcccg agcgccacac gctggcgttg cccgccggaa agcgcgcccg gcttgcgttt
    10921 caggtactca cgcaggccga gaatttgcgc cgcccagttc acccgctcat caatcacctc
    10981 tttggcgatt ttttgcatct tcaggccaaa cgccatgttg tcgtaaaccg tcatatgcgg
    11041 atacaacgcg tagttctgga acaccatcgc gatattgcgt gctttggctg gaacgtcatt
    11101 cattcgtttg ccgtcgatca acagatcgcc gccgctgatc tcctcaagcc cggcaatcat
    11161 gcgcagggtg gtcgatttac cgcagcccga cgggccgaca aatacgatga actctttatc
    11221 ggcaatttcc aggttgaagt ccttcaccac atgcacctgg ttatcgtaga ttttttgaat
    11281 atgttgtaac gaaagctgag ccatgttgat tcctttgcta tacgttttgc cagaaggccg
    11341 ataaccgcga ccaggtcagg gagtccgttg agggcaacag taattgcgcc ccggttaagc
    11401 ccgcaccgat ccccaccgag cgcataccgc tggcgttaat ggcgtcaatg cccgcctgcg
    11461 catcttcaat gccgatacat gcctgcggcg gcacgcccag cccggcacag gcagcgagaa
    11521 agatttccgg atccggtttc gagtttttaa gttgggaagc atccgcgcag aaggtgaaaa
    11581 actcgcgcag ctccagcgcc gctaaaatcg tcggcgcatt cagggagacg gaagccagcc
    11641 ctaccggaat ctgctgtgca cggagctctg ccagcaaatt gcgaataccg ggcagcacag
    11701 cattaaccgt cagctcgcgc agtgagtgga cgtaaagcag atttttgcga tacgccagtt
    11761 gcgcccgctc ctgcgggtta aagtcgccct ctttgccccc gtgttgcaga atgcgccgca
    11821 gagactcatc gcggctgatc ccttttagag attcgttaaa ctgcgcatca atactgatgc
    11881 caatttcagc ggcaatctgc tgccacgcct ggaaatgcag atgcgcggta tcggtgatta
    11941 caccatccag atcgaaaatt accccttgca gtttcattca tcctcctgat gcgtggtagc
    12001 ggtcccattg aagggcaaaa taaaatcacc caaacagaaa acagattctt cagccacgga
    12061 tataagctga ccgttcaaac gcagtgaaac gggcgcagaa gttcgaatcg caatacgctg
    12121 cgcgtcgaga gtgacctgta attcgcagcc ctgccagaac agagggaaag acaactgttg
    12181 ccactgctca agtaacgccg gatttagatg caattcaccg tcacgcacgc tcaccccggc
    12241 aaaaccctga atcgccccca gccagatagc gccagttgcg gcagcatgga taccatcatc
    12301 acaactatgc ggatcagcac caagatcgat ttcagtcccc tcgcgccaga actgataact
    12361 ttgggtcagc aggccacagc gtgcagcaac aatgccgtgg attgctttac ttaatgacga
    12421 gtcgtgaata gtgcgcggtt cataaaattg cagattggca agacacgatg ccgatgagaa
    12481 ctgctctggc agcatgtaat tgagcatcac cacatcagct tgtttgagga tctgcatctc
    12541 gttcacttct gcgcgtgaat aatccagcag aatggtttgc ttccccgccg ccgctttgta
    12601 tttcgccaga ttaatcgccg gcttagtcat aaacgaatca tcctgcggca aaacaccgtc
    12661 gggctgaatt tctggcagcc gcagttcttt aaggaacatt tcggcacgat gaataaacgc
    12721 gtcgtcacta cagccaaact ggcgggcaat actcagcgcc tgctggacgt tgtaatacac
    12781 catatagctg gtgaaggcgt tattattgac atgttcggta tattcgtctg gcccaataac
    12841 atcatgaatt tccagacggt cgttaaccct caccgcgcgg ctaatccaga actttgcagt
    12901 ttccagaagt agcgccatgc cttcatgcgc aatgaaactt tcatcccccg tggtctgcca
    12961 gtattgaata accgcccagg cgatatcggc caccagatga tgttccgcct gcgccgaggc
    13021 cactttttgc cgcagcccgg tgcgaatgtt aatggcggca aattccggcg tctcttcttc
    13081 gccgttgcgt cgcgctttct cctgcgcgcc tggcaagttg tgccagcgat aacgcagtaa
    13141 acttcgggca accgtcggat cgctaaacag atggaacggc cgcaaaaata cttctgtatc
    13201 ccagaaaacg tggcctttgt agccttcgcc ggttaagcct tttgccgcaa tactgctgcg
    13261 ctcgccgtga gccggcgtca tgatgcgcag atgataaagc gcataatcta acgcttgctg
    13321 atcgtgcgca tcgccgccat ttaccgtgat acgacgtttc tgccaccatt gacgccagtt
    13381 ttctgttgat gctgcaagat gttggtcgta actctgttgc gcgcacattt caagctggcg
    13441 aagcgacacg ctgccccact cgtctaaagc agcttgcctg tcatcccgcc agtcgatcca
    13501 caccagtttt tgcaacgtca ctgtctcgcc tgcatgaagc tgcgtactgg tatgttgcag
    13561 taaacggcgc tctttggcgg tatagcattg ctgcacatca ccgctcacct ggcagcaaca
    13621 actgatggcc acatcactgc gcccatcctg ggtggtgtag atcccctgca tcaaatgctg
    13681 accaaacacc cgcacctggg tttcgtcgag atgctggcga ccgtggttgg tttgcgtggc
    13741 gtcaatgcct gttgaaatca gcactgacgc gtcggcgtcc agtggcataa tagtgatttc
    13801 cagcgcaatg agcggcagtt ggtctgccga aacaaagcga cggctggcga tggtgtaacc
    13861 tgcgccgttg ctggtacgcc agacaacgct acggcgtaat tctccactgg caaagtcaag
    13921 ttcacgctgc caggcttcgc gggataacga gaaaacctca ccatttatgg caatctccat
    13981 ccccaagata tcaggcagat tcaccagttc gttgatttca ccttttcccg cccgatgata
    14041 cagccccgcc agatacatcc ctcgcgtctg gcgcgtgtaa tcttcttcat ggctggcgcg
    14101 aagcccaaga tagccgttcc cctgcgccat cagcgatgca tacttgttca gggtatgctg
    14161 gctgaaatgg ggttctgata acgttactgg cctggtcata attccacacg cgtccctgtt
    14221 tctgcggatt gatataacgc cgccaccagt tgctggatga tgtacccctg ctcggcgtcg
    14281 gctatcatca cgggcttgcc ctgtacgtga ttgataaagg cttccatgct gcgcaaatgg
    14341 cggttgtcgt ctgccatttc ccgttgcatc agcgtcatta attcaccgtt gttatcggtg
    14401 tagatatgtg ctggaaacag cgtcgcaccg gctttatcac cacaaaagct gacgttcata
    14461 atcaactgtt cgcggatgtt gagtgcaaat gacgtttcca gccacagaat gccgccgtta
    14521 tgaaattcaa tggtgccaaa cagcgaatct tcgacgctgt aagttgccgg atcccactcg
    14581 ccaaattgac cacagctctt ttgcgtgccg atcttttgaa aactatgcgc attcacgctt
    14641 ttcaccgccg gaaagcccag cacatacatc gcagcatcca gcatatgaat gccgatgtcg
    14701 atcagcaggc caccaccctg cagttcttta ttggtaaaga caccccagcc gggaacgccg
    14761 cagcgacgca gggcgcgggc ggtggtaacg taaatctctc ccaaaacgcc gttggtcacc
    14821 tgttcacgca gctgttgcgt atcgagcgca aacgatggtg aaagtcgtag gccagcacct
    14881 tgcccagttt gcgcgcggta tcgcacattt cccacgcctg ttctgtcgtc atggcgggcg
    14941 gtttttcgca catcacatgg cagcctgctt ccagtgccat cagggtatgt tcgtaatgaa
    15001 aacggttagg tgagccgacg ctaaccacat caggtttcac cgccagcagc atggcctgcg
    15061 gatcgtccca cacggatgca ttcccgtatt tttctgccag cgcctgcgcc tgggaaaggc
    15121 ggctgtcaca gacagccacc agttccagat cgttgcgggt gcagtagtac gaagcatgaa
    15181 ccttatccgc cacctggccc gcgcctatta tcgcgacccg cagcggagag cttgtcattg
    15241 cacttttcac aaagacctct tagcaggtac gcaaccaggc caacgaatca cggtacgcct
    15301 gggcaggatc ttctgcccgg atacgccctt catacaccac ataaccctga tagttatcgg
    15361 cgcgcagctg ttcaaacagc gcgtggaaat cgagggtgcc actgcccggc tggtaacgat
    15421 ggttatcggc aatatgcaca tgaccgagca agtcacggtt gtcgtgcagc gcctgcgcca
    15481 ggttatcttc ttcgatgttc atgtgataga aatcgccgat aatctgcaca tgtttaagat
    15541 tgttttcgac gatgtaacga cgagcatcgg cgagggtgtt gatcatatga tcctgatagc
    15601 ggtttaacgg ttcgagatac accacggttc cggtacgcgc ggcgaggtca tcggcggtaa
    15661 gcggaaggta aacatgcccc acgctgccgg aaacccccca cttctgccag cgcctcgaga
    15721 atgcgttcga tctgctttaa gccattaaga cgacgctctt cgataaagtc accaatccag
    15781 ccgtcatagc cgccgcaggc ggtggtcact ggtaaaccgg tttctttgat cgccgctttg
    15841 acttcttcga tgttgttgac cagcagtttg ccgtcaatct caaaaccatc gaagcccatc
    15901 tctttgatat aacgaaatct ctcgcggata ttttccggaa agaatgcctg attctgtgtg
    15961 ccgattttca ttttttcatt cctttgaatt aaaacgtaac gcccattttg atgctctgtt
    16021 ccggatgctg atcgacatac tgcatgtagc tttccgggct gttggcaaag gtcactaccg
    16081 ggtcgattaa atcttcgcaa ttgagataac cgttcatcag cagttcccag caggtttctt
    16141 caatacgctt acggctccag cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga
    16201 aaacaatttt ggcgttattg aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg
    16261 gtttggcaaa cgcgacatag gagatggtgc cgccgtaggc caggccgcgc agcgccgatt
    16321 gcagcgcgtc ggcgtaaccg ctggtttcga taatcacatc agccccctgc ttgccggtca
    16381 gcgttttgat ctctttgcct acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt
    16441 ggcgacgggc aatatcacag cgatgggcga taggatcgac gccaattacc accgatgcgc
    16501 cagcgcgttt cgccagttgg atggcaattt gaccgatcgc gccaagccct accaccacga
    16561 caaaatcccc tacgcgtacg ttggcatcgc gcacgccgct catggcaaac tgcgccgggt
    16621 cgtaacagac ggcgtttttc caggagctgc cttgcggcat tttgcgcagc ttgtagttat
    16681 tcactgcgtt aatgatgacc gtctcggaga gcgggccgta gccgcatacg ctgtcgccca
    16741 ccgcgtagtc tgtaacgtcg ctgccgcact cgataatgtc gccaaccacc atgttgccaa
    16801 gctggaattt gccaaactca atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct
    16861 gccactcgcc gttaaagtct tcatcgataa acgggctggc ggcgcggaag tcgaccactt
    16921 ccgttccgtg tttcggtgcg ccgaaacgca cgcggatctt cacttcattg gctaaaatcg
    16981 cccgatcttc atactcaacc agcgcagcaa cacgcggtgc tgtggctact aactttttca
    17041 tgtaaaactc cttgtaaaga aactggccgc tttagccctt cacgccaccg gcggtcaaac
    17101 cactcttaat aaaacgttcc gaaagcgcat acataatcac caccggcaat gcggtcacca
    17161 gcgaggccgc catcatccgt ccccagatgt aatcaggcgt actgaacagc gcgttcaggc
    17221 ccactggtaa ggtgaaattg ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt
    17281 tccacgccac cataaagcag tagacgaaca ccgatatcag cccggacatc gccaggggca
    17341 cggtgatacg gaagatgatt tgcagacggt tgaggccatc catcatcgcc gcttcttcga
    17401 tctcatcagg gatggtgtcg aagtaacttt tcagcataaa cacggcggtg ggcagggttt
    17461 gcgtcaccat ggtaatgatc agcgccatct cggtgtcgta aataccaagc gcggtgatga
    17521 ttttgaacag aggcaccacc agcaaaatgc cagagaacat atacaccgtg taaaagctgg
    17581 cgttgatggt catccggcct ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc
    17641 cgagaaatac cgccaccacg gaggaaacca ccgacaccac catgctgtta cggaagtagt
    17701 cgacaaacgg gaaaatcatc gggttaaaaa tgtcgacgta atgctccagc gtccactgtt
    17761 gcggcagcag cgtaggatgc agcgatatcg cctctttcgc gcttttgaac gaggtcatca
    17821 gcatcacaaa aaacggaaac agcgtgatga tgagaaacag cgccagcccg cagtaaaaac
    17881 cgatacgact gagcgtgcgt ttatttgttg ccattgaggt tcacccgttt cctggtcagc
    17941 agaatgacag cgaaaataat gatgaagagc accaccgaga tcgccgccgc tttccctaag
    18001 tcattaaagg caaaggcggt tttgtagaga tagacaccaa gaatatcgac cttggttgtc
    18061 agcagataaa catcagcgaa catgtagaac atccagatgg tgcgcagggt caccaccgtc
    18121 gccaggaccg gcataattgc gggcagcgtg acgatgcgaa accgctgcca ggcattagcg
    18181 ccatccatct ctgccgcttc gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac
    18241 gagataaagg catacgggaa gtagcgccag atggcgaaca gcaccaccag cacaaagcta
    18301 ctgcccggat tatcaaacca cagcggtgcc tgctcataga gatgcagaag atcgacgccg
    18361 aggtagttca caatgccgta gccgttgttg aacatgtatt tccaggcaaa caccagcgaa
    18421 atggacggcg ttacgtagga gaggatcacc agcgaacgcg cggttttgcg caggcggaac
    18481 tcgcggttga aaaacatcgc cacggccagc ccgagcacgg tgctgcccgc caccaccagc
    18541 gccgtatacc agaccgtcat ccacagcgag tgccagaagc cgggatcgga gagaatgcgc
    18601 acatagttac tcaccccaac aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa
    18661 aaactgattt cgatattcga caccatcggc cacgccacca gaccgcccag cagtaataag
    18721 ctgggcgcga gaagcagcag cgcaaacggc atatcggaac gacctgaaaa aagcctgttc
    18781 attgcggatt cctgtcctgg gtttagtgct gttcgatcag ttcatccagc tttttctggc
    18841 tcgcttgcag cgtgccaggg agatcggctt tacccacggt gacgttatgc accatggagc
    18901 tcaccacacc agaacccgtc acatcgccca tgcgggtaaa gtttttatcc cctactacgc
    18961 caaagacctg aatatttggc agttcaccga ttagctgatt cggtagttca cccagcgcct
    19021 taataacgtc gttgtctttc caggtggcgg tagtgacaac cgctttattc accggcagcg
    19081 cagcacctgg cgacatcatc acccagtcgg caatgttgtc cgcctgctcc ataaaggtga
    19141 caaatttttc tgctgcttcg gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc
    19201 cgtagaccgc agagtttttc tcggttggca ccacgaaacc gacgtttttc gggtcgcctt
    19261 ctttaatcac agccggaagg atataggtgg agtaaatcgc catcggcgcg gtgccgttca
    19321 taaaggcgtc tttcacttcc atgatgtcgt tagaacccgg catggtgttg gcagcaaggt
    19381 cgcggtaata ggtcagcgcc tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg
    19441 cgttaaagac gttggcctag ttggataacg caaactggga gaaggattgt tccgtcagca
    19501 cgctttctgc cgtaggcagc gcaataccgt attttttatt cgccgggtca ttcagtttct
    19561 gtgcaacgtc cagcagctgt tgccagtttt tcggctcctc cagccccgct tttgccagca
    19621 catctttgcg ataccagatg ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc
    19681 taccatcttc ggtacgcaca atacgcagta cgccatcgta aaacgcgcct tcaccaacgt
    19741 tgctgatgac cgtggcaacc gctttgcgat cgataagctg ctctttatcc ataactttgg
    19801 cgtagtcatg gctggtttcg atcacttccg gcagcgagcc gctacgtgaa agggtgatga
    19861 ctttggtgtt atgagcgtct tcttccacgg gcacttgttt aacggtgata cctgggtttt
    19921 ctttttcaaa acgggcaatc aatttgctga taactgcctg acgctcctgc tccaccgaag
    19981 aatgcataaa ttcgatggat acatgggttt tattttcttc tttacagcct gaaatcaggg
    20041 cgcaggaaac cagtgctgat aacagtacga tttttgattt aatcatgaga tgttcctttt
    20101 tatttaatcc acataacctg ccaggggcgc agcgttattt cattacccaa tatatcaact
    20161 tcactaatca gatcccgacc atgcagatca gtaatattta catgctgaat atttttactg
    20221 acattaaaca atccagtcag acaattacca tcagcgttac ttctttgaat acgcattacg
    20281 gatgaattaa ccgtatcgat ggtaaaatta ttatccggat gaaactcgtt atggctgcga
    20341 cgaagtgtaa ttaaacgcga caactcatga tataccgcat gccttaatgt agcttcatcg
    20401 ttcagttctc gtgttatctc ttcactgtga tattttttac ggttaatcgc acggttatat
    20461 ccgagttttt cgacacctgc gtaatcatta cgcgagccaa gaatactttg aatatatatc
    20521 gcgggtacac cggggaaact taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt
    20581 tcttcatcgc tactctcgcg gcggcttaac gcattcatat aggtgacatt catctcatag
    20641 ggactgcgcg tgccgtcggg attatttttc cagtttact
//
LOCUS       DAOFMX010000075        20612 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.075, whole genome shotgun sequence.
ACCESSION   DAOFMX010000075 DAOFMX010000000
VERSION     DAOFMX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.075"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            96..395
                     /locus_tag="QBA20_003064"
     CDS             96..395
                     /locus_tag="QBA20_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6293505.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            405..929
                     /gene="ygaP"
                     /locus_tag="QBA20_003065"
                     /pseudo
     CDS             405..929
                     /gene="ygaP"
                     /locus_tag="QBA20_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
     gene            complement(976..1380)
                     /gene="stpA"
                     /locus_tag="QBA20_003066"
     CDS             complement(976..1380)
                     /gene="stpA"
                     /locus_tag="QBA20_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDQ6293506.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2048..2497
                     /gene="alaE"
                     /locus_tag="QBA20_003067"
     CDS             2048..2497
                     /gene="alaE"
                     /locus_tag="QBA20_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDQ6293507.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2534..2878)
                     /locus_tag="QBA20_003068"
     CDS             complement(2534..2878)
                     /locus_tag="QBA20_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDQ6293508.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3030..3260
                     /locus_tag="QBA20_003069"
     CDS             3030..3260
                     /locus_tag="QBA20_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293509.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSRGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3607..3852
                     /gene="nrdH"
                     /locus_tag="QBA20_003070"
     CDS             3607..3852
                     /gene="nrdH"
                     /locus_tag="QBA20_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDQ6293510.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3849..4259
                     /gene="nrdI"
                     /locus_tag="QBA20_003071"
     CDS             3849..4259
                     /gene="nrdI"
                     /locus_tag="QBA20_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDQ6293511.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4232..6376
                     /gene="nrdE"
                     /locus_tag="QBA20_003072"
     CDS             4232..6376
                     /gene="nrdE"
                     /locus_tag="QBA20_003072"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDQ6293512.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6386..7345
                     /gene="nrdF"
                     /locus_tag="QBA20_003073"
     CDS             6386..7345
                     /gene="nrdF"
                     /locus_tag="QBA20_003073"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDQ6293513.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7700..8902
                     /gene="proV"
                     /locus_tag="QBA20_003074"
     CDS             7700..8902
                     /gene="proV"
                     /locus_tag="QBA20_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDQ6293514.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8895..9959
                     /gene="proW"
                     /locus_tag="QBA20_003075"
     CDS             8895..9959
                     /gene="proW"
                     /locus_tag="QBA20_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDQ6293515.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10017..11009
                     /gene="proX"
                     /locus_tag="QBA20_003076"
     CDS             10017..11009
                     /gene="proX"
                     /locus_tag="QBA20_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDQ6293516.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11201..12380
                     /locus_tag="QBA20_003077"
                     /pseudo
     CDS             11201..12380
                     /locus_tag="QBA20_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12504..13241
                     /gene="ygaZ"
                     /locus_tag="QBA20_003078"
     CDS             12504..13241
                     /gene="ygaZ"
                     /locus_tag="QBA20_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDQ6293517.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13231..13566
                     /gene="ygaH"
                     /locus_tag="QBA20_003079"
     CDS             13231..13566
                     /gene="ygaH"
                     /locus_tag="QBA20_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDQ6293518.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13657..14187
                     /gene="emrR"
                     /locus_tag="QBA20_003080"
     CDS             13657..14187
                     /gene="emrR"
                     /locus_tag="QBA20_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDQ6293519.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14314..15486
                     /gene="emrA"
                     /locus_tag="QBA20_003081"
                     /pseudo
     CDS             14314..15486
                     /gene="emrA"
                     /locus_tag="QBA20_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15503..17041
                     /gene="emrB"
                     /locus_tag="QBA20_003082"
     CDS             15503..17041
                     /gene="emrB"
                     /locus_tag="QBA20_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDQ6293520.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="QBA20_003083"
     CDS             complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="QBA20_003083"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDQ6293521.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="QBA20_003084"
     CDS             complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="QBA20_003084"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDQ6293522.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19399..19827)
                     /locus_tag="QBA20_003085"
     CDS             complement(19399..19827)
                     /locus_tag="QBA20_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDQ6293523.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="QBA20_003086"
     CDS             complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="QBA20_003086"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDQ6293524.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4975 a         5274 c         5147 g         5216 t
ORIGIN      
        1 agcttacgct tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt
       61 agagtttact taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg
      121 ccagtaccga acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga
      181 ttctgtgcat gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg
      241 gactgagcgc ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca
      301 acagccaacg ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca
      361 ttatcgccac cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca
      421 tttcgccgca tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg
      481 acgctgatga atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc
      541 tggaacagtc aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg
      601 caggtaaacg caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa
      661 tatttttact cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa
      721 ataaatctca acccttgccg ttaatgcgct aggtgcagat cgctgcgggt ggtttaatat
      781 taatcggcgt tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg
      841 ttggtgccgg gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag
      901 ataagatgcc gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac
      961 atccggcctc aataattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg
     1021 agcaattggc ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc
     1081 agtgaattta tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc
     1141 agagctatta cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca
     1201 ggtgctaatt ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg
     1261 tctttcttta gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa
     1321 ttcgcgagcc atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat
     1381 aataaagaaa accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat
     1441 cacttattat atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc
     1501 tgttattttc tgaaactctc agaataattt caagattata tattagccat tttgatgagt
     1561 aatttaggtt attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca
     1621 aaaccgaagt gaataaaaat gcaatataac agaataaaag aaaacacccc tcactggtgc
     1681 tttctgctgt taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcacgac
     1741 aaacaccgaa ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat
     1801 taaaggcaag atcatatcta attgatttaa ttgaaaataa atcatatcgg catgtaaaaa
     1861 tccacaatgt acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctaactaaat
     1921 aaaaattttt aactaattga ttagtcatag acagcgatat acgctatgca aaaatgcaga
     1981 tgacaatgag atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag
     2041 cattagcatg ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat
     2101 ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt
     2161 cgaacagtct ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc
     2221 atacggtatg taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg
     2281 gataaaaaat ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc
     2341 gatcttgtta gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat
     2401 cgttgtttcg atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact
     2461 gtttaaagtc agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc
     2521 gccagtcgcg tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa
     2581 cacgcgaact aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga
     2641 gcggaaattg ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg
     2701 aactgcgttc ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc
     2761 gattaacata gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat
     2821 cgacagtaaa accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat
     2881 ttgatcctcg ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt
     2941 tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa
     3001 aattacatcg aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg
     3061 atgatggcgt gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg
     3121 tattgaagtc caggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc
     3181 aggcgttgct gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg
     3241 cgcgcgatgc cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg
     3301 gtacagcagc tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt
     3361 gcaaaaatga taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt
     3421 gcctggtgaa tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat
     3481 cactatttgc tatatattgt gtggttgaat cttttttcaa ctacatctag tatctctgta
     3541 tcaacagaga gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata
     3601 cgaatcatgc gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa
     3661 cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa
     3721 gcagcggaag cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat
     3781 cttagctggt ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg
     3841 gccagtgcat gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta
     3901 tcgaacgttt aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg
     3961 tagacgagcc ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg
     4021 tgccacgaca ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg
     4081 ttattgcttc tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg
     4141 cccggaaatg cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata
     4201 tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac
     4261 gcaggaaacg atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg
     4321 tcgcattcag ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg
     4381 tccgaacagt gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg
     4441 ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc
     4501 ccacgcacac accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac
     4561 cagctatacg ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt
     4621 cacgatggtg gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga
     4681 tgaaatgctg tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca
     4741 gcagcgcggc gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat
     4801 tggtcgggcg gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt
     4861 gctgtcgaat ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg
     4921 cgtaattccg gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc
     4981 gcgtcagggg gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct
     5041 cgacacgaaa cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt
     5101 ggtgatcccg gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc
     5161 gccctatgac gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca
     5221 ctatgacgaa ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt
     5281 cttccagcga ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga
     5341 cacagtaaac cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga
     5401 aattttgcag gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg
     5461 ccatgatatt tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga
     5521 tttttcccgc acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca
     5581 tatccgcagc gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg
     5641 gcagatgaat ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc
     5701 cctggatttc accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat
     5761 gttgctggcg cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag
     5821 tggtgaatat ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg
     5881 cgaactgttt gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg
     5941 cgacgacgtg atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg
     6001 gtctatctct tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga
     6061 gatacgcaaa gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga
     6121 aaatctggcg ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta
     6181 cgcggaagcg actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac
     6241 cgccaccact cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac
     6301 gctctattac atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt
     6361 ctcctgtgca ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa
     6421 caagatatct gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct
     6481 accagaaaag gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga
     6541 acaacaactg acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt
     6601 tatcggcgcg ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc
     6661 gaatatcagc tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct
     6721 gtgccagacc aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca
     6781 gcgaaaagct cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat
     6841 cgccagtgtg tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt
     6901 ttccagccgc ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga
     6961 agcagtccac ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct
     7021 gggacaacgt gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa
     7081 cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc
     7141 gtttctctgt tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc
     7201 cgcagaaatg gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga
     7261 aaatcacgat ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac
     7321 agaagatgaa gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca
     7381 gggaaataat tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact
     7441 gatactctgt tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat
     7501 gacaaaaaat atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg
     7561 cgatttgctc gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca
     7621 gtatgttagg gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat
     7681 aaaggaatca ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt
     7741 tggtgagcat ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat
     7801 tctggaaaaa actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg
     7861 cgagatattt gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct
     7921 caatcgcctg attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa
     7981 aatatccgac gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc
     8041 ctttgcctta atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc
     8101 cggaattaat gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct
     8161 ggaaaattat gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg
     8221 attagcccgc gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc
     8281 gctcgatcca ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca
     8341 tcagcgcacc attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg
     8401 aattgccatt atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa
     8461 taatccggcg aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt
     8521 cagtgcgaaa gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt
     8581 cggcccacgt tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat
     8641 cgaacgcggt aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac
     8701 gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac
     8761 gcctcttagc gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga
     8821 cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg
     8881 tgagggggta aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca
     8941 gtgccgcgca atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg
     9001 gtgctgactg gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc
     9061 cgttccataa aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg
     9121 ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg
     9181 gtttccagca attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca
     9241 tcacctggca gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca
     9301 tcggcgcaat cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc
     9361 tgctgttctg tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag
     9421 cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc
     9481 tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca
     9541 tctttgcgct gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg
     9601 atctgattga agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc
     9661 agttaccgct ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc
     9721 tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc
     9781 gcggtatcgg tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc
     9841 tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca
     9901 accgtcgctg gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac
     9961 tctgcacttg cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc
    10021 gacatagcgt actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg
    10081 ccgatctgcc gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa
    10141 ccttccagac gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac
    10201 ccagcgaagt ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca
    10261 ccgccgtgaa ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga
    10321 aattttatcg tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga
    10381 aaaccgccga ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca
    10441 aactgttcga taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg
    10501 gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc
    10561 ataatcaggg gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca
    10621 aaccggtgtt ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga
    10681 aagatgtggt ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata
    10741 ccaaactgcc gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca
    10801 acaaagcctg gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc
    10861 cagtggccga tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg
    10921 atattcaggg ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg
    10981 tgaatgaggc gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc
    11041 tcatccgccg ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca
    11101 aaccacatcg cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt
    11161 attgatttcc agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga
    11221 gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa
    11281 ctattacgcc cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc
    11341 ggcaggcttt attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt
    11401 tcccctcggt gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc
    11461 gggcggtatg ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc
    11521 attaaccggt ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc
    11581 ttcaccggat aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat
    11641 attgctggca cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt
    11701 ttgggttgct tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca
    11761 aatgaaatca gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt
    11821 catcagcgat aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt
    11881 cagcattctc tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga
    11941 tggtgacatt ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc
    12001 gggcggtttt gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac
    12061 tttcatggct ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc
    12121 tggtgctgga cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa
    12181 tacatcctga tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg
    12241 gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt
    12301 gtctggctgg cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc
    12361 gtcaggaagc cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg
    12421 tgttaagaag cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt
    12481 aacaatcgtt aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg
    12541 cgaccttcat ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg
    12601 cctttgcgtt cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt
    12661 tctcctgcat catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg
    12721 ggagtagttt gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt
    12781 atggcccgtc actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat
    12841 gggcgtttgg cctgacggat gaggtttttg ccgccgccac cgcaaaactg gtacgcaata
    12901 atcgccgctg gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg
    12961 tatttggtac ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg
    13021 ttgaagctgc attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt
    13081 tccagcgcaa acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa
    13141 cgctattttc tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt
    13201 taatccaggc attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta
    13261 ctggttggcg cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat
    13321 gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg
    13381 atatgcgctc tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc
    13441 gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt
    13501 atcatcccaa cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt
    13561 atataaccca caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt
    13621 tactatatcg gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt
    13681 gaacaaatgc taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt
    13741 ctgactcgtc tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg
    13801 aaggctcagg ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa
    13861 aaccacagta ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg
    13921 acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac
    13981 gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt
    14041 ttaccaccgc agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa
    14101 gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac
    14161 ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa
    14221 aagaaaaaat gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc
    14281 agccgatgag ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc
    14341 ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg
    14401 ctctttataa ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc
    14461 gaagaaaccg atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct
    14521 ggcagcgtga cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg
    14581 gtcactctcg acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct
    14641 tccagcgttc gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt
    14701 gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg
    14761 ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt
    14821 gcccaggcgc aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg
    14881 gggactaaac tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc
    14941 tagctggcgc tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt
    15001 gccgtgcaac cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc
    15061 accaatatgt gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt
    15121 cagccggtca ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg
    15181 gttggtctgg atatgggcac aggtagcgcg ttctcactgc ttccagcgca aaatgcgacc
    15241 ggtaactgga tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag
    15301 ctggagcaat acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac
    15361 cgtgacggtc aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg
    15421 cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct
    15481 ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt
    15541 cattatgacg attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc
    15601 taacgtggcg atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg
    15661 ggtaatcact tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc
    15721 aaagcgcgtc ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc
    15781 ttgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg
    15841 gattgtcgcc gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc
    15901 agccaaacgc tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg
    15961 cggcccgatc ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat
    16021 caacgtgccg attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga
    16081 aacccgcacc gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat
    16141 cggcagcctg cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga
    16201 aattatcatt cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct
    16261 gaccgacgat aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg
    16321 ctgcttgtgt atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca
    16381 gttgttgcag gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt
    16441 agggattatt ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat
    16501 gcggcggctg gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta
    16561 tacctttgaa ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt
    16621 tgcggtggtc tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga
    16681 acgactggcg gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg
    16741 cacgtcgata accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac
    16801 tgagtcggta aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct
    16861 tgggatgacg caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct
    16921 gattatttcc gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg
    16981 gctggtgtgg tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta
    17041 agtacaacta agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta
    17101 ctgactagat gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt
    17161 tgatgcgtac ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt
    17221 gcatctggta agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca
    17281 ctttcagcac gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg
    17341 taccaatcag actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct
    17401 ctacaccatt accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat
    17461 ggatccctct ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg
    17521 tgattgcgtc gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca
    17581 tccgggtatg atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt
    17641 ttttaaaaat tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca
    17701 tttgttatca tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt
    17761 tcttttctgt caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc
    17821 catttcctgc tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag
    17881 aatttccagc ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt
    17941 tccgccaata ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg
    18001 attatcgaag caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat
    18061 gctatccagc gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag
    18121 taacgggaac tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc
    18181 aaggatcacc cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg
    18241 tgcatcagcc agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc
    18301 tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc
    18361 gccacgtaac agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat
    18421 cggcgcatac agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc
    18481 gtctttctca ataccaatct tcgcgtactc ttccgatggc gtcttgattg cctgtttaag
    18541 gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt
    18601 ggtatagccg agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca
    18661 ctcggttttc tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc
    18721 cggagatgca ccaaacagat aaggaatgac ccaaccgaaa cgatagtaat tgcggataac
    18781 gcggaaatag cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt
    18841 cgcttgccag aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat
    18901 cagcgcgccg tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt
    18961 agaagtgccg tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact
    19021 taacggccac atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat
    19081 aaaggtcagc atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc
    19141 cgcaaaatcg gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc
    19201 tgttgttgcc agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg
    19261 tatccccttt aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg
    19321 gatcaaattg acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc
    19381 atatgtgcag gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac
    19441 tgcaacatag cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat
    19501 gcgcatccat cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa
    19561 tagcgtgact gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt
    19621 tttacgcaat ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc
    19681 cattgttgct gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac
    19741 cagcacgact tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa
    19801 cgagaaaagc gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct
    19861 gcacgggccg cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc
    19921 acgcccatac gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg
    19981 tgatcagcag cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc
    20041 tcagcgatgg cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt
    20101 ttcaccactt caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct
    20161 tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt
    20221 gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac
    20281 tgaagaccgt agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg ctccgtatcc
    20341 aggattgtgc catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg
    20401 caagtcgaaa aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa
    20461 ggagaagaaa attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg
    20521 aaattttttg gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    20581 agccgagtac tctatccagc tgagctacgg at
//
LOCUS       DAOFMX010000076        20570 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.076, whole genome shotgun sequence.
ACCESSION   DAOFMX010000076 DAOFMX010000000
VERSION     DAOFMX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.076"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            409..1239
                     /locus_tag="QBA20_003087"
     CDS             409..1239
                     /locus_tag="QBA20_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDQ6293525.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="QBA20_003088"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="QBA20_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDQ6293526.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="QBA20_003089"
     CDS             complement(2368..3828)
                     /locus_tag="QBA20_003089"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDQ6293527.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="QBA20_003090"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="QBA20_003090"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDQ6293528.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="QBA20_003091"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="QBA20_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDQ6293529.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="QBA20_003092"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="QBA20_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDQ6293530.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYLETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="QBA20_003093"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="QBA20_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDQ6293531.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="QBA20_003094"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="QBA20_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDQ6293532.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="QBA20_003095"
     CDS             complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="QBA20_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDQ6293533.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSSFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="QBA20_003096"
     CDS             complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="QBA20_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDQ6293534.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12470..13009)
                     /locus_tag="QBA20_003097"
     CDS             complement(12470..13009)
                     /locus_tag="QBA20_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6293535.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="QBA20_003098"
     CDS             complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="QBA20_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDQ6293536.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(14103..14699)
                     /gene="fimE"
                     /locus_tag="QBA20_003099"
     CDS             complement(14103..14699)
                     /gene="fimE"
                     /locus_tag="QBA20_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDQ6293537.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(15177..15779)
                     /gene="fimB"
                     /locus_tag="QBA20_003100"
                     /pseudo
     CDS             complement(15177..15779)
                     /gene="fimB"
                     /locus_tag="QBA20_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            17233..17949
                     /gene="nanC"
                     /locus_tag="QBA20_003101"
     CDS             17233..17949
                     /gene="nanC"
                     /locus_tag="QBA20_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDQ6293538.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            17969..19075
                     /gene="nanM"
                     /locus_tag="QBA20_003102"
     CDS             17969..19075
                     /gene="nanM"
                     /locus_tag="QBA20_003102"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDQ6293539.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            19140..20120
                     /locus_tag="QBA20_003103"
     CDS             19140..20120
                     /locus_tag="QBA20_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDQ6293540.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         5474 a         4909 c         4674 g         5513 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg tagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc atcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaatc gccaccccgc ccgcactgct cacaggtgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc agataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    11101 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    11161 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcctggacca
    11221 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    11281 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    11341 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    11401 cgacgcgata cgtccctggc ggtaattctt gcccattttc aaaactcgat aaatcggcca
    11461 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    11521 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    11581 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    11641 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    11701 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    11761 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    11821 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    11881 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    11941 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    12001 tgataattgc gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg
    12061 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    12121 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    12181 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    12241 tattttcatc attatttgtc acggcaagtt gcacttgttt ttgccctgcc ggataaatta
    12301 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    12361 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    12421 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    12481 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    12541 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    12601 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    12661 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    12721 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    12781 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    12841 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    12901 cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag
    12961 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    13021 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    13081 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    13141 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    13201 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    13261 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    13321 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    13381 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    13441 cgaacctgtc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    13501 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    13561 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    13621 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    13681 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    13741 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    13801 ttggcttaat attctattat tatctttatt tatagatttt tatattgcat gaggtagttt
    13861 ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg
    13921 gaggtgatgt gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt
    13981 tttgtcgttt tttaatattt ttatgcttga gaaaaaatac gtaacttatt tatgatatgg
    14041 acagtttggc cccaattgtc ttgtgtttat ttgtttttta actttattat caataagtta
    14101 aatcaaacct cttctctttt taatttttcg tttatgagat tatttctttc ccataatccg
    14161 gcaaaacgag cagcattact ggcggtataa cgcacagtat ggcgaatatt tcgatgcccg
    14221 agataatcct gaattaaacg ggtatctgca ccacgctccg ccagttcata accgcaagca
    14281 tgccttaaca tatgaggatg agtctgcgtt acggttccag cttcaatacc ggcatcgcga
    14341 ataatgcgat aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat aaatatagcg
    14401 tcggtccggt cagcgccttt ccagttagca cgttcctggg tccagcgttc cacggcttca
    14461 cgctcatcaa aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt
    14521 attcgacctt cattaaggtc aaggtcctga taatgcagat caagcagttc actaatacgc
    14581 atcccatgcc gatatgccaa cagaataaga caataatctc tggctcccgt tgccccgtaa
    14641 caaaccgcct gcatcatggc ctgaacttct ttaccggtaa gataacgacg tttactcaca
    14701 atagtagtac tcctaactga gatatatttg aatgaatacc tataggaaac ctcaatcggt
    14761 caaaattagc ccaatagata aaaagataac cacacacgaa gttatgtggc tatcagtcaa
    14821 ttacattaaa catcatacaa aaataaaata tcactgatgt gacaacatct attttcattg
    14881 atttgattca atataacaat agattacaga ggattaactt ccagttactt aaaaaataaa
    14941 gaaaaaagtg tggaaacagg agaatttaag tgatgtgtaa acttatgaat gattttgttt
    15001 atttattttg aagaacataa accattagat tttgtatggg tatggttaac ttaatgttta
    15061 ttatgataat taagaatcac acttacaata acatcaatta ataaatggtt aatcattttt
    15121 gatatatcgt aagaataatg tagtttttaa caccatccct ggtatctcaa ctatctctat
    15181 aaaacagcat gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa acgccctgca
    15241 ttgctggcgg tataccagac agtatgacga atattgcgat gcccaagata atcctagata
    15301 agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatagcg taacatgtgc
    15361 ggatgaatct ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac
    15421 tgttgccgag aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc
    15481 ggataagaag tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc
    15541 gggtgcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga
    15601 tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa
    15661 agcaaaatca gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt
    15721 gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc
    15781 gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt
    15841 tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca
    15901 gttcttctga gcgaacttaa atggagtagc gatagttact tataataact ttgccataca
    15961 aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa
    16021 taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta
    16081 acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttaaac tagccgtctc
    16141 aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt
    16201 aatttcttag tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg
    16261 ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat atgggggaac
    16321 gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta
    16381 agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc
    16441 atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac
    16501 aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgagta
    16561 aacccttatt ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct
    16621 aaataaatta tttataaaac atatagttaa aagaaaatct ttagtttttg aagaccaccg
    16681 caagtgttcg tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt
    16741 gacgcgaatt gcaaatctgg ttttgttgta tggattgtgt gatttttgat ctggtataac
    16801 aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac
    16861 atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga
    16921 aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat
    16981 ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta
    17041 atctggatct ctgtttattt aaataatgtt aaaagagaat tttcacagga gaccttatac
    17101 aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc aaaaaaatat
    17161 tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa
    17221 tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg
    17281 tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc
    17341 cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc
    17401 atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat
    17461 gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg
    17521 ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc
    17581 tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac
    17641 cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat
    17701 gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa
    17761 cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc ctttgttcta
    17821 caataccata tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag
    17881 ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt
    17941 aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat
    18001 gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag
    18061 tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc
    18121 atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc
    18181 tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg
    18241 cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa
    18301 ccccaaaacc aatagctggg ttaaattgat gtcgcacgcg ccgatgggca tggcgggtca
    18361 tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt
    18421 caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat
    18481 caacgcccac tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc
    18541 ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc
    18601 tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc
    18661 aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa
    18721 taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa
    18781 tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa attaccagaa
    18841 cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg
    18901 gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc
    18961 attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt
    19021 gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt
    19081 cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc gaaaattata
    19141 tgaacgcaat aatatcaccc gattattact atgttcttac cgttgctggt cagtctaatg
    19201 ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct cattccagaa
    19261 ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgccatgt cactttaacg
    19321 acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt tatcaccatc
    19381 ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg catattgcac
    19441 ggaaattact gcccttcatt cctgataatg cagggattct tatcgttccg tgttgccgtg
    19501 gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac ggagccagcc
    19561 atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc agcagaacgc
    19621 gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg
    19681 aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat catatggttg
    19741 aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt actgacgcgc
    19801 cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat tcgtatgaag
    19861 ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc gacttccagc
    19921 aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg
    19981 gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg cgaagcagtc
    20041 atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa gcaattttgc
    20101 agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt gttcgttctc
    20161 agagtagaca acaaaaaaca aatgagatca aggtaatatc tcttcacttg cgggctcgaa
    20221 cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc accgcataaa
    20281 ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc cttttcgatt
    20341 atggctcgga aatctacgaa catctacaca gtttactgga acgttttggg ccacagcgtt
    20401 cgcagcaccg tcctgatatg ccgttctggc gattataagt cgacggattc tggcaaatag
    20461 acgatgatga tatgcttaat gatgttcaaa gaaattaggc cattattata ttattgcttg
    20521 cattttcttt cattcctaaa agcacccgcc attaatccca ggcgcattat
//
LOCUS       DAOFMX010000077        20543 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.077, whole genome shotgun sequence.
ACCESSION   DAOFMX010000077 DAOFMX010000000
VERSION     DAOFMX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.077"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="QBA20_003104"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="QBA20_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDQ6293541.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="QBA20_003105"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="QBA20_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HDQ6293542.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="QBA20_003106"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="QBA20_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDQ6293543.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="QBA20_003107"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="QBA20_003107"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDQ6293544.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="QBA20_003108"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="QBA20_003108"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDQ6293545.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="QBA20_003109"
     CDS             4449..4814
                     /locus_tag="QBA20_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDQ6293546.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTNWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="QBA20_003110"
     CDS             4964..5482
                     /locus_tag="QBA20_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDQ6293547.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="QBA20_003111"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="QBA20_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="QBA20_003112"
     CDS             6713..7195
                     /locus_tag="QBA20_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDQ6293548.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="QBA20_003113"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="QBA20_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDQ6293549.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="QBA20_003114"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="QBA20_003114"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDQ6293550.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="QBA20_003115"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="QBA20_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDQ6293551.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="QBA20_003116"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="QBA20_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDQ6293552.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="QBA20_003117"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="QBA20_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDQ6293553.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="QBA20_003118"
     CDS             10939..11730
                     /locus_tag="QBA20_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDQ6293554.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="QBA20_003119"
     CDS             11751..13037
                     /locus_tag="QBA20_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDQ6293555.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="QBA20_003120"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="QBA20_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDQ6293556.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="QBA20_003121"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="QBA20_003121"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDQ6293557.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="QBA20_003122"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="QBA20_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDQ6293558.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="QBA20_003123"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="QBA20_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDQ6293559.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="QBA20_003124"
     CDS             complement(16985..17275)
                     /locus_tag="QBA20_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDQ6293560.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="QBA20_003125"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="QBA20_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDQ6293561.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="QBA20_003126"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="QBA20_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDQ6293562.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18933
                     /gene="ssrA"
                     /locus_tag="QBA20_003127"
     tmRNA           18570..18933
                     /gene="ssrA"
                     /locus_tag="QBA20_003127"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19138..20379
                     /locus_tag="QBA20_003128"
     CDS             19138..20379
                     /locus_tag="QBA20_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDQ6293563.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5308 a         5366 c         4817 g         5052 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctagaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac caactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttaccta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctaa tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaaatagtcg
    18661 caaacgacga aaactacgct ttagcagctt aataacctgc ttagagccct ctctccctag
    18721 cctccgctct taggacgggg atcaagagag gtcaaaccca aaagagatcg cgtggaagcc
    18781 ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt tgttagtggc gtgtccgtcc
    18841 gcagctggca agcgaatgta aagactgact aagcatgtag taccgaggat gtaggaattt
    18901 cggacgcggg ttcaactccc gccagctcca ccaaaattct ccatcggtga ttaccagagt
    18961 catccgatga agtcctaaga gcccgcacgg cgcaagccct gcgggctttt ttgtgctccg
    19021 aatttgtccc gcgaagtctg atgccaacta attaaatccg atccttttag gcaccttgtt
    19081 aggcacttca taaagctata ttgtctttga ggtgcctaaa actatggaaa cccggcaatg
    19141 gcaagacaaa ccaaacctct atccgttaag gaaatcgaat ctgctaaacc caaggaagcg
    19201 gactacgttc tctatgatgg cgatggcctt gagctactca ttaaatccag cgggagtaaa
    19261 atctggcagt ttcgctacat tcgccctgtc accaagacac gagcaaagaa aagcataggc
    19321 ccttacccgt cagttaccct tgccgatgcc agaaactacc gtgcagagtc ccgctcactc
    19381 ctagctaaac agattgatcc gcaggaacat cagcaagaac aattgcgtag ttcgctggaa
    19441 gccaaaacca atactttcca gctcgtagct gaacgttggt ggaatgtgaa gaaagccagt
    19501 gtgacagagg actatgcgga agatatctgg cgctctcttg aaagagatgt ctttcctgcg
    19561 attggcgacg ttagcgttac agatattaaa gctcatacac tggttcaggc cgtacaaccg
    19621 gttcaggcca gaggagcact ggaaacggtt cgtcgcctgt gtcaacgtat taatgaggtc
    19681 atgatctatg cccagaacac agggctgatt gatgctgttc ccagcgttaa tatcggtaaa
    19741 gccttcgaaa agcctcagaa aaagaacatg cccagcattc gaccggatca gctatctcaa
    19801 ctgatgcaga caatgcgaac agccagcatt agcctttcca cacgctgcct gttcatgtgg
    19861 caacttctta ctattacccg ccctgccgaa gcggctgaag ctcgctggga agaagtagac
    19921 atagaagcgc aagagtggaa gattcccgca gcacgcatga aaatgaaccg cgaccatact
    19981 gttccattgt cagatgaagc aatagctgtg ctggagatga tgaaaccgct aagcggcaac
    20041 cgagaattta tctttcctag ccgtatcaag cccaaccagc ctatgaacag tcagactgtt
    20101 aacgcctcac taaaacgcgc aggttttggt ggtgtgcttg tttcgcatgg tctgagatca
    20161 attgccagta cggctcttaa tgaacaaggc tttccgcctg atgccattga ggcagcattg
    20221 gcccatgtgg ataagaatga ggttcgtcgt gcttataacc gcagcgatta cctggaacag
    20281 cgtcgcccga tgatgcaatg gtgggcaaat tttgtgatgg cagcggatcg tggaagcatg
    20341 attgaaggtg ggataaaagg aatgaagcta gttgggtgac taaatagctg cgcggcatag
    20401 tgtcccactt tcagccagcc cttgcaggtt aagccaccag acagtaaacg gcgggtgtcg
    20461 cgttattagg cgcagctatt tagtagtaat attaggattg gtgattctgc actccagaag
    20521 ttcggtaatg ctgccaactt act
//
LOCUS       DAOFMX010000078        20289 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.078, whole genome shotgun sequence.
ACCESSION   DAOFMX010000078 DAOFMX010000000
VERSION     DAOFMX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.078"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="QBA20_003129"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="QBA20_003129"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDQ6293564.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="QBA20_003130"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="QBA20_003130"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDQ6293565.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3000)
                     /locus_tag="QBA20_003131"
     CDS             complement(1729..3000)
                     /locus_tag="QBA20_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6293566.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3183..4388)
                     /locus_tag="QBA20_003132"
     CDS             complement(3183..4388)
                     /locus_tag="QBA20_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6293567.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAEHFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="QBA20_003133"
     CDS             complement(4488..4844)
                     /locus_tag="QBA20_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDQ6293568.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKYLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="QBA20_003134"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="QBA20_003134"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="QBA20_003135"
     CDS             complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="QBA20_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDQ6293569.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6774..9830
                     /locus_tag="QBA20_003136"
     CDS             6774..9830
                     /locus_tag="QBA20_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDQ6293570.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9827..10237
                     /gene="menI"
                     /locus_tag="QBA20_003137"
     CDS             9827..10237
                     /gene="menI"
                     /locus_tag="QBA20_003137"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDQ6293571.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10337..10525
                     /locus_tag="QBA20_003138"
     CDS             10337..10525
                     /locus_tag="QBA20_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDQ6293572.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11073..11441
                     /gene="sufA"
                     /locus_tag="QBA20_003139"
     CDS             11073..11441
                     /gene="sufA"
                     /locus_tag="QBA20_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDQ6293573.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11450..12937
                     /gene="sufB"
                     /locus_tag="QBA20_003140"
     CDS             11450..12937
                     /gene="sufB"
                     /locus_tag="QBA20_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDQ6293574.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12947..13693
                     /gene="sufC"
                     /locus_tag="QBA20_003141"
     CDS             12947..13693
                     /gene="sufC"
                     /locus_tag="QBA20_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDQ6293575.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13668..14939
                     /gene="sufD"
                     /locus_tag="QBA20_003142"
     CDS             13668..14939
                     /gene="sufD"
                     /locus_tag="QBA20_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDQ6293576.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14936..16156
                     /gene="sufS"
                     /locus_tag="QBA20_003143"
     CDS             14936..16156
                     /gene="sufS"
                     /locus_tag="QBA20_003143"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDQ6293577.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16169..16585
                     /gene="sufE"
                     /locus_tag="QBA20_003144"
     CDS             16169..16585
                     /gene="sufE"
                     /locus_tag="QBA20_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDQ6293578.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16734..17738
                     /gene="ldtE"
                     /locus_tag="QBA20_003145"
     CDS             16734..17738
                     /gene="ldtE"
                     /locus_tag="QBA20_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDQ6293579.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="QBA20_003146"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="QBA20_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDQ6293580.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="QBA20_003147"
     CDS             complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="QBA20_003147"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDQ6293581.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5316 a         4860 c         5176 g         4937 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccaacca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcctgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtatccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaaagg tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgccag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgtactgt aatagatccc tgtagcttta cctttagcgt aagggaaatg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatatttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
     5221 atatcaatta gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat
     5281 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg
     5341 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     5401 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga
     5461 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     5521 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga
     5581 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     5641 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     5701 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     5761 gtgccgccag cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     5821 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     5881 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     5941 tggtgccaat ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt
     6001 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     6061 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     6121 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     6181 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     6241 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     6301 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     6361 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     6421 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     6481 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     6541 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     6601 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     6661 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
     6721 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
     6781 cacagatttc cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
     6841 agcaacaagg ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga
     6901 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
     6961 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
     7021 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
     7081 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
     7141 aggccggggt gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg
     7201 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
     7261 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
     7321 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc
     7381 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
     7441 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
     7501 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
     7561 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
     7621 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
     7681 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
     7741 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat gtgcctgaca
     7801 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
     7861 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg
     7921 tgattggctg gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca
     7981 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
     8041 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
     8101 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
     8161 tccgtccggc actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
     8221 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
     8281 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
     8341 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
     8401 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
     8461 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
     8521 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
     8581 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
     8641 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
     8701 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
     8761 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt
     8821 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
     8881 gttatttacg cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga
     8941 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
     9001 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac
     9061 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag
     9121 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
     9181 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
     9241 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
     9301 cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg
     9361 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
     9421 aacgtggcga gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac
     9481 atccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta
     9541 ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
     9601 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
     9661 accatgaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
     9721 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
     9781 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
     9841 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
     9901 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
     9961 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    10021 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    10081 caatgctaat cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt
    10141 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    10201 gtgctgttcg tcacgattga cgactgccat tttgtgacgt tgtcaccaga aaagtgtgac
    10261 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    10321 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    10381 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    10441 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    10501 tgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    10561 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    10621 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    10681 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    10741 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
    10801 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    10861 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    10921 tgcctgttaa cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt
    10981 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
    11041 atacgctgaa agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc
    11101 cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg
    11161 agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct
    11221 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    11281 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca
    11341 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    11401 cccagaatga atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa
    11461 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaatt ataaagaagg
    11521 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    11581 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    11641 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    11701 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    11761 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    11821 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    11881 cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat
    11941 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    12001 cggcaccgtg gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc
    12061 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    12121 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga
    12181 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    12241 gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    12301 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    12361 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
    12421 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    12481 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    12541 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    12601 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    12661 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    12721 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    12781 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    12841 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    12901 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    12961 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
    13021 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    13081 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    13141 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    13201 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    13261 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    13321 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    13381 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    13441 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    13501 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    13561 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
    13621 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    13681 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    13741 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    13801 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca
    13861 ttgcgggaga tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc
    13921 ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg
    13981 gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag
    14041 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    14101 gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag
    14161 aggtgaacac tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg
    14221 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta
    14281 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    14341 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    14401 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    14461 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    14521 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    14581 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    14641 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    14701 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    14761 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    14821 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    14881 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    14941 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    15001 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    15061 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag
    15121 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    15181 ccgttcggcg gaagagctgg tgttcgtccg cggcacgacg gaagggatca atctggtcgc
    15241 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    15301 gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    15361 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    15421 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    15481 actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    15541 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    15601 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    15661 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    15721 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    15781 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    15841 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    15901 tctcactctc tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    15961 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    16021 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    16081 ggccatgtat aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca
    16141 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    16201 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    16261 ccagcgtctg ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg
    16321 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    16381 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    16441 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    16501 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    16561 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    16621 tgccggatgg tcaatgttta cactgacctg cacaggagtg ttttggtttc aggtgaacat
    16681 aagcaccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    16741 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    16801 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    16861 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa
    16921 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    16981 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    17041 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    17101 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    17161 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    17221 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    17281 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    17341 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    17401 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    17461 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    17521 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    17581 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    17641 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    17701 aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt gattttttat tgcttctggt tatcgattaa ataaaaaaag
    18301 cgcccatcag ggcgcttcga tatacaaatt aattcacaaa agcaatatta caggacgtga
    18361 acagatgcgg tgttagtagt gccgctcggt accagtgcac cagaaaccat aactacgacg
    18421 tcacctttgt gtgccagacc gctctgcaga gccagttctt tacccagacg gtagaaatca
    18481 tcagtagaag tgatctcttt aaccagctgc ggcacaacgc ctttgctcag taccaactga
    18541 tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg catccgggaa gtatttacgt
    18601 actgcgcgag cagatttacc gccctgggta gcaaccacga tcagcggagc atccagtttt
    18661 tcagcagttt caacggcacc acggcatacc gcttcggtga tgcgcagttt acggttgtca
    18721 ttgttgaact cgagacggct gttcatcacg cggtcggtac gttcgcagat ggtcgccatg
    18781 atagaaaccg cttccagcgg gtatttacct tttgcggatt caccagacag catcactgcg
    18841 tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg
    18901 tttttgatca tggaatccag catctgggtc gcagtgataa cgactttacg tgcacggata
    18961 catttttcga tcatcatctt ctgggcgaag ataacttctt ctaccgggat ttctacaccc
    19021 aggtcgccac gcgcaaccat gatgccgtca gaggcttcga ggatttcgtc gaagttgttg
    19081 aggccttcct ggttttcgat tttggagatg atgtggatgt tttcgccgcc gtgcgctttc
    19141 aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa taaaggaagc agcaacaaag
    19201 tctacgcctt tttcgcaacc aaagatcagg tcctgtttgt ctttttcagc cagtgctggc
    19261 agagcaatgg aaacgccagg caggttcaca cctttgtttt cgcccaggtc accgttgttc
    19321 agcactttac agataacttt gttaccttca atggcggtaa cttccatacc gatcagacca
    19381 tcgtcaacca gtacggtgtt gccaacagac aggtcagtag tgaaaccttc atacgttacc
    19441 gcaaccattt cgctgttgcc gataacagat ttatcagtgg tgaaagtaaa ggtctgacca
    19501 gctttcagag aaacatcgtt accgccttcc agtttcatgg tgcggatttc cggacctttg
    19561 gtatcaagca ggatagcggc ggttttacca gttttgctca tcacgttgcg cagattctga
    19621 atgcgctgac cgtgttctgc atagtcacca tgagagaagt tcagacgcat aacgttcatg
    19681 ccagcgtcca gcattttagc taacatctct tcagattcgg ttttcggtcc gatggtgcaa
    19741 acaattttgg tctttttcat gacagtctta gtctttaagt tgagaaggat gggagaaact
    19801 tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta cgaaagtggt ggtgcctcgc
    19861 tctaaggata ggtgacatcg aaaaagcgtg cagaggaatg tgtgcttgtg tctcagccca
    19921 acgtgagtgt ctgtttttga gtcgttgagt tctacagtcc aaagtgctga aaccattcaa
    19981 gagtcaattg gcgcgcatta tacgctgaaa ttaatccaaa aggaaaacaa aaacagcgtt
    20041 tcaaaaaata tacaacttgt tagacaataa attgttatca aagatttaac gttccaggga
    20101 aaaggttacg caaaaacttc gctgtttgcg caacgtctgg tgccgaagtt gtcagtgtga
    20161 ggataagtga aagttcatcc ggtgtataaa tatatcatcg ctcaattgct acagttaaaa
    20221 taatttatcg cgttatttgt ctgattttgt ttgatttttg tttaattggt tagattgata
    20281 tatgctgat
//
LOCUS       DAOFMX010000079        19698 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.079, whole genome shotgun sequence.
ACCESSION   DAOFMX010000079 DAOFMX010000000
VERSION     DAOFMX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.079"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="QBA20_003148"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="QBA20_003148"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDQ6293582.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="QBA20_003149"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="QBA20_003149"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDQ6293583.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="QBA20_003150"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="QBA20_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDQ6293584.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="QBA20_003151"
     CDS             6038..6658
                     /locus_tag="QBA20_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDQ6293585.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="QBA20_003152"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="QBA20_003152"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDQ6293586.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="QBA20_003153"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="QBA20_003153"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDQ6293587.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="QBA20_003154"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="QBA20_003154"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDQ6293588.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGACFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="QBA20_003155"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="QBA20_003155"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDQ6293589.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="QBA20_003156"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="QBA20_003156"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="QBA20_003157"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="QBA20_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="QBA20_003158"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="QBA20_003158"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDQ6293590.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="QBA20_003159"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="QBA20_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDQ6293591.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="QBA20_003160"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="QBA20_003160"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDQ6293592.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="QBA20_003161"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="QBA20_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDQ6293593.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="QBA20_003162"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="QBA20_003162"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDQ6293594.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="QBA20_003163"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="QBA20_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDQ6293595.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5506 c         5031 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc acgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcagcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctaccagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct atgccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaagaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cacgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggtgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcatcgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAOFMX010000080        19375 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.080, whole genome shotgun sequence.
ACCESSION   DAOFMX010000080 DAOFMX010000000
VERSION     DAOFMX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.080"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="QBA20_003164"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="QBA20_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDQ6293596.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHYMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="QBA20_003165"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="QBA20_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDQ6293597.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="QBA20_003166"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="QBA20_003166"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDQ6293598.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="QBA20_003167"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="QBA20_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDQ6293599.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="QBA20_003168"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="QBA20_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDQ6293600.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="QBA20_003169"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="QBA20_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDQ6293601.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="QBA20_003170"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="QBA20_003170"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDQ6293602.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="QBA20_003171"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="QBA20_003171"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDQ6293603.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="QBA20_003172"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="QBA20_003172"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDQ6293604.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="QBA20_003173"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="QBA20_003173"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDQ6293605.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="QBA20_003174"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="QBA20_003174"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDQ6293606.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="QBA20_003175"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="QBA20_003175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDQ6293607.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="QBA20_003176"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="QBA20_003176"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDQ6293608.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="QBA20_003177"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="QBA20_003177"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDQ6293609.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="QBA20_003178"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="QBA20_003178"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDQ6293610.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="QBA20_003179"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="QBA20_003179"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDQ6293611.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="QBA20_003180"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="QBA20_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDQ6293612.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="QBA20_003181"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="QBA20_003181"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDQ6293613.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4768 a         4858 c         5122 g         4627 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc attatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cataagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgta tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccg
//
LOCUS       DAOFMX010000081        19212 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.081, whole genome shotgun sequence.
ACCESSION   DAOFMX010000081 DAOFMX010000000
VERSION     DAOFMX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.081"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..2000
                     /gene="pbpC"
                     /locus_tag="QBA20_003182"
                     /pseudo
     CDS             1..2000
                     /gene="pbpC"
                     /locus_tag="QBA20_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
     gene            2150..2581
                     /gene="ndk"
                     /locus_tag="QBA20_003183"
     CDS             2150..2581
                     /gene="ndk"
                     /locus_tag="QBA20_003183"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDQ6293614.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2731..3885
                     /gene="trmG/rlmN"
                     /locus_tag="QBA20_003184"
     CDS             2731..3885
                     /gene="trmG/rlmN"
                     /locus_tag="QBA20_003184"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDQ6293615.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4170..5183
                     /gene="rodZ"
                     /locus_tag="QBA20_003185"
     CDS             4170..5183
                     /gene="rodZ"
                     /locus_tag="QBA20_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDQ6293616.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5210..6337
                     /gene="ispG"
                     /locus_tag="QBA20_003186"
                     /gene_synonym="gcpE"
     CDS             5210..6337
                     /gene="ispG"
                     /locus_tag="QBA20_003186"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDQ6293617.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6440..7714
                     /gene="hisS"
                     /locus_tag="QBA20_003187"
     CDS             6440..7714
                     /gene="hisS"
                     /locus_tag="QBA20_003187"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDQ6293618.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7732..8352
                     /gene="yfgM"
                     /locus_tag="QBA20_003188"
     CDS             7732..8352
                     /gene="yfgM"
                     /locus_tag="QBA20_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDQ6293619.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8363..9541
                     /gene="bamB"
                     /locus_tag="QBA20_003189"
     CDS             8363..9541
                     /gene="bamB"
                     /locus_tag="QBA20_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDQ6293620.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9659..11131
                     /gene="der"
                     /locus_tag="QBA20_003190"
     CDS             9659..11131
                     /gene="der"
                     /locus_tag="QBA20_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDQ6293621.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11201..11416
                     /locus_tag="QBA20_003191"
     CDS             11201..11416
                     /locus_tag="QBA20_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDQ6293622.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11413..12783)
                     /gene="xseA"
                     /locus_tag="QBA20_003192"
     CDS             complement(11413..12783)
                     /gene="xseA"
                     /locus_tag="QBA20_003192"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDQ6293623.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12945..14411
                     /gene="guaB"
                     /locus_tag="QBA20_003193"
     CDS             12945..14411
                     /gene="guaB"
                     /locus_tag="QBA20_003193"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDQ6293624.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14480..16057
                     /gene="guaA"
                     /locus_tag="QBA20_003194"
     CDS             14480..16057
                     /gene="guaA"
                     /locus_tag="QBA20_003194"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDQ6293625.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16152..16691)
                     /locus_tag="QBA20_003195"
     CDS             complement(16152..16691)
                     /locus_tag="QBA20_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDQ6293626.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16707..17225)
                     /locus_tag="QBA20_003196"
     CDS             complement(16707..17225)
                     /locus_tag="QBA20_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDQ6293627.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17536..17727)
                     /locus_tag="QBA20_003197"
     CDS             complement(17536..17727)
                     /locus_tag="QBA20_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDQ6293628.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17745..17897
                     /locus_tag="QBA20_003198"
     CDS             17745..17897
                     /locus_tag="QBA20_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293629.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18079..>19212
                     /gene="pdeF"
                     /locus_tag="QBA20_003199"
     CDS             18079..>19212
                     /gene="pdeF"
                     /locus_tag="QBA20_003199"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDQ6293630.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4531 a         4672 c         5221 g         4788 t
ORIGIN      
        1 ggacttttta taccccgcag gggtcataac atgcttgaca acttaatagg ggcacgttac
       61 cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg ggttaatcca
      121 ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat ttccggtggc
      181 agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac atttggtggc
      241 aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa gcgtgaaatt
      301 ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat cggtgcggca
      361 agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc ggcaatgctg
      421 gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga gcgtgccgaa
      481 gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc ccgtgagcag
      541 gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc gcaactggca
      601 ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt tactacgctg
      661 gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg gcggttgcca
      721 ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt tcgcggctgg
      781 gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat ggtcaatgcg
      841 atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct ggatgaaggc
      901 ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga ttatcgacca
      961 ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct ggtgcaaaac
     1021 tggaagatat ggcggcagcg tataccgcgt ttgctcgcca cggcaaggca ggcaaattgc
     1081 gcttacagcc tgacgatccg ctgcttgaac ggcctttaat gtcgtcgggg gcggcgtgga
     1141 tcattcgccg gattatggct gatgaagcgc aacccttgcc ggatagtgcc ttgccgcgcg
     1201 tcgccccact ggcatggaaa acgggcacca gctatggcta tcgtgacgcc tgggcgattg
     1261 gggttaacgc tcgctatgtc attgggatct ggactggcag acctgacggc acgcccgttg
     1321 ttggtcagtt tggctttgcc agtgccgtac cattgttgaa tcaggtcaat aatatcttac
     1381 tgtcgcgcag tgcgaatctg ccggaagacc cgcgcccgaa ctcagtcact cgtggcgtta
     1441 tctgctggcc gggcggacag tctttgccgg aaggtgacgg taactgtcgc cgccgcctgg
     1501 caacctggct gctggatggc agccagccgc caacgctgtt actgtcagag caggaaggga
     1561 ttaatggcat tcgcttcccc atctggctgg atgacaaagg gcagcgcgtc gccgctgact
     1621 gcccgcaggc gaagcaagaa atggtaaatg tctggccgct accgctggaa ccctggctgc
     1681 ccgcatcaga acgtcgcgct gtgcgcttac caccggcctc gacaatttgc ccgccatacg
     1741 gtcacgatgc ccagctccct ctgcagctga caggcgttcg cgatggcgcg attattaaac
     1801 gtttaccggg cgcagcggaa gcgactttgc cgttgcaatc aagtggaggg gcaggtgaac
     1861 gctggtggtt tctgaatggc gaaccgttaa ctgaacgcgg gcgcaacgtg accctgcatt
     1921 tgacggataa aggtgattat caattgctgg tgatggatga tgtggggcaa atcgcgacag
     1981 tgaaatttgt catgcaatag tctgttttgt ccctgattgt tgctaaaact cattttattt
     2041 taaaaaaaat gttaccttca tcaatagtca acggccctgt tgctcattat aatccgcgcc
     2101 atctcgtacg ctggtacaga caacaacaga acaatttaca gaggtaaaaa tggctattga
     2161 acgtactttt tccatcatca aaccgaacgc ggtagcaaaa aacgtcattg gtaatatctt
     2221 tgcgcgcttt gaagctgcag ggttcaaaat tgtcggcacc aaaatgctgc atctgaccgt
     2281 tgaacaggca cgtggctttt atgctgaaca cgatggaaaa ccgttctttg atggtctggt
     2341 tgaattcatg acctctggcc cgatcgtggt ttccgtgctg gaaggtgaaa acgccgttca
     2401 gcgtcaccgc gatctgctgg gcgcgaccaa tccggcaaac gcactggctg gtactctgcg
     2461 cgctgattac gctgacagcc tgaccgaaaa cggtacccac ggttctgatt ccgtcgaatc
     2521 tgccgctcgc gaaatcgctt atttcttcgg cgaaggcgaa gtgtgcccgc gcacctgtta
     2581 ataatttcgt aaatgccgcg tgcaaacgtg gcatccgtgc gccagaattt gtacaatgca
     2641 gcgcccccgg acgagcagcc gctcaccggg gcgtttcttt tttcaaccct ccaggggcca
     2701 taacgtgtaa taacgaggcc ggaatagcat atgtctgaac aattagtcac acctgaaaac
     2761 gtcaccacga aagatggaaa aatcaacctg ctggatctca accgtcagca gatgcgggag
     2821 ttttttaaag atttaggtga aaaaaccttc cgcgccgatc aggtgatgaa gtggatgtat
     2881 cactattgct gcgacaactt tgatgagatg accgacatca acaaagtgtt gcgcggcaaa
     2941 ctgaaagagg tggcggaaat ccgcgcaccg gaagtggttg aagaacagcg ttcatccgac
     3001 ggcaccatta aatgggcgat cgccgttggc gatcagcgcg tcgaaacggt gtatatcccg
     3061 gaagacgacc gtgccacgct ctgcgtctct tcgcaggtgg ggtgtgcgct ggagtgtaaa
     3121 ttctgttcca ccgcccagca gggctttaac cgcaacctgc gggtatcgga aattattggc
     3181 caggtgtggc gtgcggcgaa aatcgtcggc gcagcaaaag tcaccggtca gcgcccaatc
     3241 actaacgtcg tgatgatggg catgggcgag ccgctgctca acctgaacaa cgtcgttccg
     3301 gcgatggaaa tcatgcttga tgatttcggt tttggcctgt ctaaacgtcg cgtgacgctt
     3361 tccacttccg gtgtagttcc ggcgctggat aaactgggcg atatgatcga cgttgcactg
     3421 gcaatttccc tgcacgcgcc gaacgacgaa attcgtgacg aaattgtgcc gatcaacaaa
     3481 aagtacaata tcgaaacgtt ccttgctgcg gtgcgccgtt atctggagaa atccaacgcc
     3541 aatcagggcc gagtcactat tgaatacgtg atgcttgacc acgttaacga cggcactgaa
     3601 cacgcgcacc aactggcgga actgctgaaa gatacgccgt gtaagatcaa cctgatcccg
     3661 tggaacccgt tcccggacgc gccgtatgga cgcagttcga acagccgtat cgaccgtttc
     3721 tcaaaagtgc tgatgagcta cggtttcacc actattgtgc gtaaaacgcg tggtgatgat
     3781 atcgatgctg cctgtggtca gttggcgggc gatgttatcg accgtacgaa acgtactctg
     3841 cgtaaacgga tgcagggtga agccatcgat attaaagcgg tctgatagcc attacgccac
     3901 ggttacttta tgtaccgtgg cgtaatcttt tatgaatgaa gtattaagtt gcaattgtct
     3961 ggtcaatatt aacggtgcgt ttttgctgac tttaaggcag tatgtaacga tgcaacagta
     4021 agttagcctc agtgaatgtg ggctttgtca cgagcacaca gacggtctta tactgtatga
     4081 taacggttaa cttaacggat gtttcgcggt gtgggtgagc atgatggttc accggcatct
     4141 caattctcat ttaaacgtac ctgcagcgaa tgaatactga agccacgcac gaccaaaatg
     4201 aagcacttac taccggcgct cgcctgcgta atgctcgcga acaactagga cttagtcagc
     4261 aggccgttgc cgagcgactt tgcctgaagg tttccacggt acgcgacatt gaagaagata
     4321 aggcacccgc cgatcttgct tcaacattcc tgcgcggata tatccgctct tatgcgcgtc
     4381 tggtacatat tccagaagaa gaactgctgc cagggctgga aaagcaggct ccacttcggg
     4441 ctgcaaaagt tgcgccaatg cagagttttt ccctcggtaa acgccgcaaa aaacgcgacg
     4501 gctggctaat gaccttcacc tggctggtgt tgtttgtggt tatcggcctg agcggtgcct
     4561 ggtggtggca agaccacaaa gctcagcagg aagagatcac cactatggcc gatcaatctt
     4621 cggcggaact gagcagtaat agcgagcagg ggcagagtgt tccgttaaat acgtcgacaa
     4681 ctacagaccc ggctacaacg tccacgccgc cagcgtctgt ggatactacc gcaaccaaca
     4741 cgcaaacacc tgccgtaact gcgccagcac cagctgttga tccgcaacag aatgcggttg
     4801 tttcgccttc gcaggcaaat gttgataccg ccgcgacccc ggtaccaacg gcagcaacaa
     4861 cgccagatgg tgctgcgccg ttgccaaccg atcaggctgg cgtgaccacg ccggcggctg
     4921 atccgaatgc gctggtgatg aactttactg ccgattgctg gctggaggtc actgatgcta
     4981 ccggtaaaaa attgtttagc gggatgcagc gtaaagacgg taatttgaat ttaaccggcc
     5041 aggcaccgta taaactgaaa attggtgcgc cagccgcagt acagatccag tatcaaggga
     5101 aacctgtcga tctgagtcgt tttatcagaa ctaaccaggt tgcgcgtctg accctcaatg
     5161 ccgaacaatc accggcgcag taacagacgg gtaacgcggg agatttttca tgcataacca
     5221 ggctccaatt caacgtagaa aatcaacacg tatttacgtt gggaatgtgc cgattggcga
     5281 tggtgctccc atcgccgtac agtccatgac caatacgcgt acgacagatg tcgaagcaac
     5341 ggtcaatcaa atcaaggcgc tggaacgcgt tggcgctgat atcgtccgtg tctccgtacc
     5401 gacgatggac gcggcagaag cgttcaaact catcaaacag cgggttaacg tgccgctggt
     5461 ggctgacatc cacttcgact atcgcattgc gctgaaagta gcggaatacg gcgtcgattg
     5521 tctgcgtatt aaccctggta atatcggtaa tgaagagcgt attcgcatgg tggttgactg
     5581 tgcgcgcgat aaaaacattc cgatccgtat tggcgttaac gccggatcgc tggaaaaaga
     5641 tctgcaagaa aagtatggcg aaccgacgcc gcaggcgttg ctggaatccg ccatgcgtca
     5701 tgttgatcat ctcgatcgcc tgaacttcga tcagttcaaa gtcagcgtga aagcgtctga
     5761 cgtcttcctc gctgttgagt cttatcgttt gctggcaaaa cagatcgatc agccgctgca
     5821 tctggggatc accgaagccg gtggtgcacg cagcggggca gtaaaatccg ccattggttt
     5881 aggtctgctg ctgtcggaag gcatcggcga cacgctgcgt gtatcgctgg cggccgatcc
     5941 ggtcgaagag atcaaagtcg gtttcgatat tttgaaatcg ctgcgtatcc gttcgcgagg
     6001 gattaacttc atcgcctgcc cgacctgttc gcgtcaggaa tttgatgtta tcggtacggt
     6061 taacgcgctg gagcaacgcc tggaagatat catcactccg atggacgttt cgattatcgg
     6121 ctgcgtggtg aatggcccag gtgaggcgct ggtttctaca ctcggcgtca ccggcggcaa
     6181 caagaaaagc ggcctctatg aagatggcgt gcgcaaagac cgtctggaca acaacgatat
     6241 gatcgaccag ctggaagcac gcattcgtgc gaaagccagt cagctggacg aagcgcgtcg
     6301 aattgacgtt cagcaggttg gaaaaataat aacgtgatgg gaagcgcctc gcttcccgtg
     6361 tatgattgaa cccgcatggc tcccgaaaca ttgagggaag cgttgagggt tcatttttat
     6421 attcagaaag agaataaacg tggcaaaaaa cattcaagcc attcgcggca tgaacgatta
     6481 cctgcctggc gaaacggcca tctggcagcg cattgaaggc acactgaaaa acgtgctcgg
     6541 cagctacggt tacagtgaaa tccgcttgcc gattgtagag cagaccccgc tattcaaacg
     6601 tgcgattggt gaagtcaccg acgtggttga aaaagagatg tacacctttg aggatcgcaa
     6661 tggcgacagc ctgactctgc gccctgaagg gacggcgggc tgtgtacgcg ccggcatcga
     6721 gcatggtctt ctgtacaatc aggaacagcg tctgtggtat atcgggccga tgttccgtca
     6781 cgagcgtccg cagaaagggc gttatcgtca gttccatcag ttgggctgcg aagttttcgg
     6841 tctgcaaggt ccggatatcg acgctgaact gattatgctc actgcccgct ggtggcgtgc
     6901 gctgggtatc ttcgaacacg taactcttga gctgaattct atcggttcgc tggaagcacg
     6961 cgccaattac cgcgatgcgc tggtggcatt ccttgagcag cataaagaaa agctggacga
     7021 agactgcaaa cgccgcatgt acactaaccc gctgcgcgtg ctggattcaa aaaatccgga
     7081 agtgcaggcg cttctcaacg acgctccggc attaggtgat tatctggacg aggaatctcg
     7141 tgagcatttt gccggtctgt gcaaactgct ggagagcgcg gggatcgctt acaccgtaaa
     7201 ccagcgtctg gtgcgtggtc tggattacta taaccgtacc gttttcgagt gggtgactaa
     7261 cagtctcggc tcccagggca ccgtgtgtgc aggcggtcgt tatgacggtc ttgtggaaca
     7321 actgggcggt cgtgcaacac cggctgtcgg ttttgcgatg ggcctcgaac gtcttgtatt
     7381 gttagtacag gccgttaatc cggaatttaa agccgatcct gttgtcgata tatacctggt
     7441 ggcttcaggt gctgatacac aatctgcggc tatggcatta gctgagcgtc tgcgtgatga
     7501 attactgggc gtgaaattga tgaccaacca cggcggcggc aactttaaga aacagtttgc
     7561 ccgtgctgat aaatggggtg cccgcgttgc tgtggtgctg ggtgagtctg aagtggctaa
     7621 cggcacagca gtagtgaagg atttgcgctc tggtgagcaa acggcagttg cgcaggatag
     7681 cgtagccgcg catttgcgca cgttactggg ttaaggaagg agaaggacag cgtggaaatt
     7741 tacgagaacg aaaacgacca ggtagaagcg gttaaacgct tttttgctga aaatggcaaa
     7801 gcactggctg ttggggggat tttgggcgtt ggcgcactga ttggctggcg ctactggaac
     7861 agccatcagg ttgattctgc acgctccgct tctcttgcct atcaaaatgc ggttaccgca
     7921 gtgagcgaag gcaaaccgga tagcatcccg gcggcggaaa aatttgctgc tgaaaataaa
     7981 aatacttatg gtgcgctggc ttctttggaa cttgcgcagc aatttgttga caaaaatgaa
     8041 ctagagaaag ctgccgccca gttacaacag gggctggcag acacgagcga tgaaaatctc
     8101 aaagccgtga taaatctgcg tcttgctcgc gttcaggtac agctcaagca ggctgatgcc
     8161 gcgctgaaaa cccttgatac catcaaaggt gaagggtggg ctgccattgt tgccgacttg
     8221 cgtggtgaag cattgctgag caaaggtgat aagcaaggtg cgcgtagtgc atgggaagca
     8281 ggcgtgaaaa gcgatgttac tccggcactg agcgaaatga tgcagatgaa aattaataat
     8341 ttgtccatct gagagggacc cgatgcaatt gcgtaaatta ctgctgccag gactgctttc
     8401 cgttaccctt ttaagcggct gttcgctgtt taacagcgaa gaagatgtgg taaagatgtc
     8461 cccattgcca accgttgaaa accagtttac gccgaccacg gcgtggagca cttccgttgg
     8521 tagcggcatt ggcaacttct attccaatct tcatccggca ctggcggaca acgttgtcta
     8581 tgcagcggac cgcgctggtt tggtaaaagc gctgaatgcg gatgatggca aagaaatctg
     8641 gtctgtcagc ctggccgaga aagatggctg gttctctaaa gagcctgcat tactttctgg
     8701 cggtgtgacc gtgtctggtg ggcatgtcta cattggcagc gaaaaggcgc aggtttacgc
     8761 gctgaatacc agcgatggta ctgtggcatg gcaaactaaa gtcgcgggtg aagcactttc
     8821 gcgcccggtg gtcagcgacg gtctggtgtt aatccacacc agtaacggtc agttacaagc
     8881 gctgaacgaa gctgacggcg ctgtcaaatg gacagttaac ctcgatatgc cttcgctctc
     8941 tttgcgtggc gagtctgcgc cggcaacggc ttttggtgcg gccgtcgtgg gtggcgataa
     9001 tggtcgcgtc agcgcagtgc tgatggaaca gggccagatg atttggcagc agcgtatttc
     9061 ccaggcgacc ggttctaccg aaattgaccg tctgagcgat gttgacacga ctcccgtcgt
     9121 tgttaacggc gttgttttcg cgctggccta taatggtaac ctgacggcgc ttgatctgcg
     9181 cagtggtcag attatgtgga aacgcgaact gggttcggtg aatgatttca tcgtcgatgg
     9241 caatcgcatc tatctggtcg atcaaaatga ccgggtgatg gcgttgacca ttgatggcgg
     9301 cgttacgctg tgggcacaaa gcgatctgct gcatcgcctg ctgacttctc cggtgctgta
     9361 taatggcaac ctggtggtcg gtgacagtga aggttatctg cactggatta acgtcgaaga
     9421 tggtcgtttc gttgcccagc aaaaagttga tagttccggt ttccagactg aaccggttgc
     9481 cgctgacggc aaactgctga tccaggcaaa agacggaacc gtgtactcta ttacacgtta
     9541 atcgtctctg tcgttcactt tgaaaacggc tcctggacag gggccgtttt cctgttttta
     9601 acaacgacgc gaatatagcg tgcgttgtct gatgatttat aaaaatgagg ctttaaacat
     9661 ggtacctgtg gtcgcgcttg tcgggcgccc taacgtagga aaatccacgt tatttaaccg
     9721 tctaactcgc acccgagatg cgctggttgc ggatttcccg ggtctgactc gtgaccgtaa
     9781 gtacggtcgt gcggaaattg aaggccgtga gtttatctgt attgataccg gcgggattga
     9841 tggcacagaa gacggtgtag aaacccgcat ggcggaacag tcgctgctgg cgattgaaga
     9901 agcggacgtc gtactgttta tggtggatgc gcgcgcgggc ctgatgccgg cagatgaagc
     9961 gattgccaaa catctgcgct cccgtgaaaa accgaccttc ctggtggtaa acaaaactga
    10021 cggtctggat cccgatcagg cagtggttga tttctactcg cttggtttag gtgaaatcta
    10081 cccgatcgcc gcgtctcacg gtcgtggcgt attaagtctg ctggagcatg tgctgctgcc
    10141 gtggatggaa gatctcgcac cgcaagagga agtcgacgaa gacgctgaat actgggcgca
    10201 atttgaagcg gaagagaacg gcgaagaaga agaggaagac gacttcgacc cgcaaagtct
    10261 gccgatcaaa ctggcgattg tgggtcgtcc gaacgtaggt aagtctacac tcactaaccg
    10321 tattcttgga gaagagcgcg ttgttgttta cgacatgcct ggcacgacgc gtgacagcat
    10381 ctacatccca atggaacgcg atggacgtga gtatgtgctc attgacaccg ctggcgtacg
    10441 taaacgcggc aaaatcaccg atgctgtaga gaaattctcc gtaatcaaaa cgttgcaggc
    10501 cattgaagac gccaacgtgg tgatgttagt gattgatgcg cgcgaaggta tttccgatca
    10561 ggatctctcg ctgctgggct ttattctcaa tagtgggcgc tcacttgtca ttgtggtgaa
    10621 taagtgggat ggcctgagcc aggaagtgaa agagcaggtg aaagaaacgc tggacttccg
    10681 tctgggcttt atcgattttg ctcgtgtgca ctttatctct gccttgcacg gcagtggtgt
    10741 tggtaacttg tttgaatcag tacgtgaagc gtatgacagc tccacccgtc gtgtggggac
    10801 ctctatgctg acgcgcatca tgacgatggc tgttgaagat caccaaccgc cgctggtacg
    10861 cggtcgtcgt gtgaagctga aatatgccca cgctggtggt tataacccgc cgattgtggt
    10921 gattcacggt aatcaggtga aagacctgcc tgattcctac aagcgctact tgatgaacta
    10981 cttccgcaaa tcgctggacg taatgggatc gccgattcgt attcagttca aagaagggga
    11041 aaacccgtat gcgaacaagc gtaacaccct gacgccaacc cagatgcgta aacgtaagcg
    11101 tctgatgaag cacatcaaga aaaataaata attatccgta agtattttat ctgccattcc
    11161 ctctatgaat gtagagggaa tggctaccgg aggaattcat atggaactgc attgtccaaa
    11221 atgtcagcat gtgcttgatc aggataacgg ccatgcgcgt tgcccatcct gcggggaatt
    11281 tattgaaatg aaagctcttt gtccagactg ccatcaaccg ttgcaggtgt taaaagcctg
    11341 cggtgcagta gattacttct gtcagcacgg gcatggcttg atatcgaaaa aacgcgttga
    11401 attcgtgctg gcttaatgaa cctttttacg cgattttttt accggctgga tgttttttac
    11461 ttcactttct atccagccgt cttccagacg tgtggttagc atttcacccg ttttaacttg
    11521 cttcactttt ttcagtacat tgccgtcagt agcagtagta acgctgtatc cacgcgccag
    11581 cgttgacagt gggcttacgg cttcgaggtg cgttactgca ttaccgaaac gttcacgcgt
    11641 ggcgctaagc tgtgcgcgca ggatttctgc taaacgatat tccagttgct gaatgcgcgt
    11701 ttgcgtgcga tgaatcttcg gctgtggatt ttgctgattc agccgctgtg ttaatcgctg
    11761 ctgctgttgc ccggcacgct taagctgatt ttccagcgca aagctcattc gtttttgcag
    11821 gcgttcaagc atggtttgct ggcgtgccag ccggagctgc ggatgctgct gctgcaagcg
    11881 atgatgaatc tgcgtaaagc gacgtgtacg gttggcgaga taataatcca tcgccatctc
    11941 cagccgttga cgggccgatt gcacctggcg cagtaactct tgctgattac ggctcactac
    12001 ttcagcggcg gcagacggcg ttggcgcacg cagatcggca acaaaatcgg caatggtcac
    12061 atccgtctca tgcccgacgg cgctgacgac cggaatgcgg ctggcaaaaa tcgcccgcgc
    12121 tacgcgttcg tcgttaaaac tccataaatc ttccagcgaa ccgccgccgc gcccaacgat
    12181 caacacgtcg cactcattgc gctgattcgc cagttcaatg gcgcgaacaa tttgccccgg
    12241 cgcgtcatcg ccctgaacgg aggtggggta gatgatcacc ggtagagaag gatcgcgacg
    12301 ttttaacaca tgcaaaatat catgtagcgc agcaccggtt tttgaggtga tcacaccaac
    12361 gcaatgcgca ggggagggaa gtggtttttt gtattgctga tcgaacaaac cttcagcctg
    12421 caacttcgct ttgagctgtt cgtacttctg ttgcagcagc ccttcaccgg ccggctgcat
    12481 actctcaacg attatctggt agtcgccgcg cggctcgtag agcgtaatat tggcgcgaac
    12541 taaaacttgt tgcccatgct gcggacggaa ggtgacccgg cggttgctgt tgcggaacat
    12601 cgcgcagcgt acctgggcgg tgtcgtcttt gagtgtaaag taccagtgac cggaagccgg
    12661 ttgtgtgaaa ttagagattt cgccgctgat ccaaacctgt cccatctcat gctcaagcag
    12721 cagacgaacc gtttgattca ggcgactaac ggtaaaaatt gcaggggatt gagaaggtaa
    12781 catgtgagcg agatcaaatt ctaaatcagc aggttattca gtcgatagta acccgccctt
    12841 cggggatagc aagcattttt tgcaaaaagg ggtagatgca atcggttacg ctctgtataa
    12901 tgccgcggca atatttatta accactctgg tcgagatatt gcccatgcta cgtatcgcta
    12961 aagaagctct gacgtttgac gacgttctcc tcgttcctgc tcattctacc gttctgccga
    13021 atactgctga cctcagcacc cagctgacga aaactattcg tctgaatatc cctatgcttt
    13081 ccgcagcaat ggataccgta acggaagcgc gcctggctat tgctctggct caggaaggcg
    13141 gtatcggctt tatccacaaa aacatgtcca ttgaacgcca ggcagaagaa gttcgccgtg
    13201 tgaaaaaaca cgaatctggt gtggtgactg atccgcagac tgttctgcca accacgacgc
    13261 tgcgcgaagt gaaagaactg accgagcgta acggttttgc gggctatccg gtcgttaccg
    13321 aagaaaacga actggtgggt attatcaccg gtcgtgacgt gcgttttgtt accgacctga
    13381 accagccggt tagcgtttac atgacgccga aagagcgtct ggtcaccgtg cgtgaaggtg
    13441 aagcccgtga agtggtgctg gcaaaaatgc acgaaaaacg cgttgaaaaa gcgctggtgg
    13501 ttgatgacga attccacctg atcggcatga tcaccgtgaa agacttccag aaagcggaac
    13561 gtaaaccgaa cgcctgtaaa gacgagcaag gccgtctgcg tgttggtgct gcagttggcg
    13621 caggtgcggg taacgaagag cgtgttgacg cgctggttgc cgctggcgtt gacgttctgc
    13681 tgatcgactc ctcccacggt cactcagaag gcgttttgca gcgtatccgt gaaacccgtg
    13741 ctaaatatcc ggatctgcaa atcatcggcg gcaacgtggc aacagctgca ggtgcacgcg
    13801 ctttggcaga agctggttgc agcgcggtta aagtcggtat cggccctggt tctatctgta
    13861 cgactcgtat cgttactggc gtaggtgttc cgcagatcac tgccgttgct gacgcagttg
    13921 aagccctgga aggcaccggt attccggtta tcgcagatgg tggtattcgc ttctccggcg
    13981 acatcgccaa agctatcgcc gctggcgcaa gcgcggtgat ggtaggttcc atgctggcgg
    14041 gtactgaaga atctccgggt gaaatcgaac tctaccaggg ccgttcttat aaatcttacc
    14101 gtggtatggg ttccctgggc gcgatgtcca aaggttcctc tgaccgttat ttccagagcg
    14161 ataacgctgc cgacaaactg gtgccggaag gtatcgaagg tcgcgtagct tataaaggtc
    14221 gcctgaaaga gatcattcac cagcagatgg gcggcctgcg ctcctgtatg ggtctgaccg
    14281 gctgtggtac tatcgacgaa ctgcgtacta aagcggagtt tgtacgtatc agcggtgcgg
    14341 gcattcagga aagccacgtt cacgacgtga ccattactaa agagtccccg aactaccgtc
    14401 tgggctcctg attctcttcg cccgatttca tgtcgggcga tttatattat ctgtttcact
    14461 tgcctcggaa taagcgtcaa tgacggaaaa cattcataag catcgcatcc tcattctgga
    14521 cttcggttct cagtacactc aactggttgc gcgccgcgtg cgtgagctgg gtgtttactg
    14581 cgaactgtgg gcgtgggatg tgacagaagc acaaattcgt gacttcaatc caagcggaat
    14641 tattctttcc ggcggcccgg aaagtactac tgaagaaaac agtccgcgtg cgccgcagta
    14701 tgtctttgaa gcaggcgtac cgatattcgg cgtttgctat ggcatgcaga ccatggcaat
    14761 gcagttgggc ggtcacgttg aagcctctaa cgaacgtgaa tttggctacg cgcaggtaga
    14821 agtcgtaaac gacagcgcac tggttcgcgg tatcgaagat gcgctgaccg cagacggtaa
    14881 accgctgctc gatgtctgga tgagccacgg cgataaagtt accgctatcc cgtccgactt
    14941 cgtcaccgta gccagcaccg aaagctgccc gtttgccatt atggctaacg aagaaaaacg
    15001 cttctatggc gtacagttcc acccggaagt gactcacacc cgccagggta tgcgcatgct
    15061 ggagcgtttt gtgcgtgata tctgccagtg tgaagccctg tggacgccag cgaaaattat
    15121 cgacgatgct gtagctcgca tccgcgagca ggtaggcgac gataaagtca tcctcggcct
    15181 ctctggtggt gtggattcct ccgtaaccgc aatgctgctg caccgtgcta tcggtaaaaa
    15241 cctgacttgc gtattcgtcg acaacggcct gctgcgtctc aacgaagcag agcaggttct
    15301 ggatatgttt ggcgatcact ttggtcttaa cattgttcac gtaccggcag aagatcgctt
    15361 cctgtcagcg ctggctggcg aaaacgatcc ggaagcaaaa cgtaaaatca tcgggcgcgt
    15421 tttcgttgaa gtattcgatg aagaagcgct gaaactggaa gacgtgaagt ggctggcgca
    15481 gggcaccatc taccctgacg ttatcgaatc tgcggcttct gcaaccggta aagcacacgt
    15541 catcaaatct caccacaacg tgggcggcct gccgaaagag atgaagatgg gcctggttga
    15601 accgctgaaa gagctgttca aagacgaagt gcgtaagatt ggtctggagc tgggcctgcc
    15661 gtacgacatg ctgtaccgtc atccgttccc gggaccaggc cttggcgttc gtgttctggg
    15721 tgaagtgaag aaagagtact gtgacctgct gcgccgtgct gacgccatct tcattgaaga
    15781 actgcgtaaa gcggacctgt acgacaaagt cagccaggcg ttcaccgtct tcctgccagt
    15841 acgttccgtt ggcgtaatgg gcgatggtcg taagtatgac tgggttgtct ctctgcgtgc
    15901 tgtcgaaacc atcgacttta tgaccgcaca ctgggcgcat ctgccgtacg atttcctcgg
    15961 tcgcgtttcc aaccgcatta tcaatgaagt gaacggtatt tcccgcgtgg tgtatgacat
    16021 cagcggcaag ccgccagcta ccattgagtg ggaatgattt tgatccctgc actatgaatg
    16081 aacaaaaccc tccgttacta cagagggttt tttatcttca agaatcatag gcttgaagtt
    16141 actaacatcg attaattaaa ccagctgtcc gatttgttct cttctgcttt gcccacgctt
    16201 ttcatcagat cgcgaccgcc ttcagtcata tttctgtttg cgtcagcttc agattgcacc
    16261 acatcggttt gcgcagcttt gtgcttcagt tcctgatcga taaattcgtt ttcgcgctta
    16321 acgcgggctt cttctttcgc cagcgccagt ttttgtttct gaatctctaa gctgcgtagc
    16381 tcatcttcat aactttgatc gcgttttttg tccgcagtgg cttcggcgtc cagtttatcc
    16441 tgacgagctt tcttatttgc cgctgccgtt gccgctcttt tgttcgcggc ggcctgggca
    16501 tttgcgcgac gttgcttctc ttgctggatt tccctgttgc gctccgcgac ccattcgtca
    16561 tgctgccttt gctcttcatt tttaccttgc tgttccgctt ctgcgacagc agagagttga
    16621 tcctgcaatg atgaggctat agccggatag cttaaggagg ctaagatggc gcaaagaaaa
    16681 actttcttca tgactcctcc tgattattag ctcttttcag gacatttggt atttggctga
    16741 atacgcgttt cgttatacgt tgtggtaata acaacggcta aacctgtcgt aaactggcac
    16801 tctttaccct cctgggtgga ggtatacact ttggtgcctt ccttatatgt taaagaaaca
    16861 ccttccacta aggttttatc attcaccata gaacccgctg ccgcgcctac agctccgccg
    16921 ccaactgccc ctgccgtcgt tccggaattg ctgccagaac cgacgttgtg gccgataaca
    16981 ccgccagcga ctgcgccaat aagcgcgccg aaggcttgtg cgttccgttt attttgggag
    17041 ttgtctacgg caacttttgc gggaagaatg gaaataatat taacggtttt agtttcttgt
    17101 ttggtattca gttgatcggt ttgataaaca tcggcggcat gatcgtcagc atttgactgg
    17161 catcctgcca gagtgaatga cgctaacatt gccacaggca gaagacattt tttaaatttc
    17221 atcactattc cttgttatac acattacgaa aaataagaca tttaagatca tcaaaagagg
    17281 aaagtaaatg tattgcgtat aaagggatag attaaattta ttaaaattca cgcagggaat
    17341 aatttataaa aatatatcat tacaacatat atattaattt tatatttatt tcgcagccaa
    17401 acaggataaa catcagtcag gacaaagacg taaaatttaa gatgaaagaa tatctgccgc
    17461 tttgataatc ccaaagagca aaagcatccg acagcgaagt gccgttatgt tctgtgaaag
    17521 tggagaaggt aaccgctaac gttgtaccgg tgattcgacg gagagtgtgg attgctgagc
    17581 ttcttttttg ctctgatggt gctgccaggc accgacggac gagtagataa aacggccaaa
    17641 gaagaagata aagctgatga gcagtacgat acgggtcatg cgactgttaa atcggtgtcg
    17701 ttttcgcata ctggttgcct gactcacaaa aggttccttg aagtatgtcc cacgctgtgg
    17761 acggtactta cattaaggca caacaggaca aaatggtcaa ttcttcgtta tgtaaaaaag
    17821 cgtcagtgga tacatatttt aatgttatgg aagttaattt aattatttac aatgatgatg
    17881 taataatgat gaattaacat gataaacgta ataattcatt aatcagggtt attttgtttg
    17941 atatatatca attggaactt tcatatgaca cttagaatca atgctctcta tagtgatgaa
    18001 taatcatcat tcgaagtcag gtgggatgcc tgtctgaata cacctccttc aggatgtggg
    18061 ggattctgct gagcatctat gaaactgaat gcaacttata taaaaatacg tgataaatgg
    18121 tgggggcttc cgctgttcct gccttcttta atcttgccca ttttcgccca cattaatact
    18181 ttcgcgcata tttcttccgg tgaggttttt ctcttttatc tgcctttggc actgatgatc
    18241 agcatgatga tgtttttcag ctgggcggca ttgccaggga tcaccttagg gatttttgtc
    18301 cgcaaatatg cagagctggg tttttacgaa acgctctcat taacggctaa ttttattatc
    18361 attatcattc tctgttgggg cggttacagg gtctttactc cccggcgtaa caacgtttca
    18421 catggtgata cccgtttaat ttcccagcgt atattctggc agatagtgtt tcctgcaacg
    18481 ctgtttctga tacttttcca gtttgctgca tttgtaggat tactggcgag cagagaaaat
    18541 ctggtcggtg tcatgccttt taacctcggg actttaatca attatcaggc cttactggtg
    18601 ggtaatctga tcggtgtccc gctgtgctac ttcatcattc gggtagtgcg aaatccattt
    18661 tatttacgta gctattattc gcaattaaaa cagcaggttg atgccaaagt caccaaaaaa
    18721 gagttcgcgc tctggctact ggcattaggt gctttattgt tgctgttatg catgccgtta
    18781 aatgaaaaaa gcacaatttt tagcaccaat tataccttgt cattattgct gcccctgatg
    18841 atgtggggag caatgcgcta tggttataag ctgatttcgc tgctctgggc ggtcgtgttg
    18901 atgatcagca tccacagcta tcaaaattac attcccattt atcctggcta taccacgcag
    18961 ctgaccataa cctcctccag ttatctggta ttctctttta ttgtcaatta tatggctgta
    19021 ctggcaactc gtcagcgagc ggtagtcaga cgcattcagc ggcttgcgta tgtggacccg
    19081 gtggttcatc tgccaaatgt tcgcgccctg aatcgcgcgt tgcgtgatgc cccctggtct
    19141 gcgctttgtt atttacgcat ccctggcatg gaaatgctgg ttaagaacta tggcatcatg
    19201 ctgcggattc aa
//
LOCUS       DAOFMX010000082        17427 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.082, whole genome shotgun sequence.
ACCESSION   DAOFMX010000082 DAOFMX010000000
VERSION     DAOFMX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.082"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(42..773)
                     /gene="rlmB"
                     /locus_tag="QBA20_003200"
     CDS             complement(42..773)
                     /gene="rlmB"
                     /locus_tag="QBA20_003200"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDQ6293631.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(953..3394)
                     /gene="rnr"
                     /locus_tag="QBA20_003201"
     CDS             complement(953..3394)
                     /gene="rnr"
                     /locus_tag="QBA20_003201"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDQ6293632.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="QBA20_003202"
     CDS             complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="QBA20_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDQ6293633.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4063..5361)
                     /gene="purA"
                     /locus_tag="QBA20_003203"
     CDS             complement(4063..5361)
                     /gene="purA"
                     /locus_tag="QBA20_003203"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDQ6293634.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5465..5662)
                     /locus_tag="QBA20_003204"
     CDS             complement(5465..5662)
                     /locus_tag="QBA20_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDQ6293635.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="QBA20_003205"
     CDS             complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="QBA20_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDQ6293636.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="QBA20_003206"
     CDS             complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="QBA20_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDQ6293637.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="QBA20_003207"
     CDS             complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="QBA20_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDQ6293638.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="QBA20_003208"
     CDS             complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="QBA20_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDQ6293639.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="QBA20_003209"
     CDS             complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="QBA20_003209"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDQ6293640.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="QBA20_003210"
     CDS             complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="QBA20_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDQ6293641.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="QBA20_003211"
     CDS             complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="QBA20_003211"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDQ6293642.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKYV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLCNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="QBA20_003212"
     CDS             complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="QBA20_003212"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDQ6293643.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="QBA20_003213"
     CDS             complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="QBA20_003213"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDQ6293644.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15981..17120
                     /gene="queG"
                     /locus_tag="QBA20_003214"
     CDS             15981..17120
                     /gene="queG"
                     /locus_tag="QBA20_003214"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDQ6293645.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17103..17156)
                     /locus_tag="QBA20_003215"
     CDS             complement(17103..17156)
                     /locus_tag="QBA20_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293646.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3955 a         4974 c         4378 g         4120 t
ORIGIN      
        1 aaaccatcca aatctggatg gcttttcata attcagagga actagctgcg ctgacgaacc
       61 gcttcaaata agcaaattcc ggttgcaacc gaaacgttca gggaagaaac acttcctgcc
      121 attgggatgc tgatcaactc atcgcaatgt tcacgggtca ggcgacgcat accttcacct
      181 tccgcgccca tcaccagcgc caggcgtccg gtcattttgc tctgatagag tgtatgatcc
      241 gcctcgcctg ccgtaccgac gatccagata ttctcttcct gcaacatacg catggtgcgc
      301 gcaaggttag tcacccgaat cagcggaacg ctttctgccg cgccgcaggc tacttttttc
      361 gccgtagcgt tgagctgtgc ggagcgatct ttcggcacaa tcaccgcatg aacgcctgcg
      421 gcgtccgcac tacgcaggca cgcgccgagg ttgtgcggat cggttacgcc gtcgaggatc
      481 agcaggaacg gttgatcgag cgaagcgatc agatccggca gatcgttttc ctgatactga
      541 cgtcctggct tcacgcgggc gataatgccc tgatgcacgg caccgtcgct tttctcgtcg
      601 agatattggc ggtttgccaa ctggataacc acgccctgag actcaagggc gtgaatcagc
      661 ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa acgttcaggg
      721 gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa tcatttcgct cattaatgta
      781 ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gcctacatga tctctgcaat
      841 atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
      901 caaagcgcat tttgtcagca atctaaccct cttcttttaa agagggtatt gatcactctg
      961 ccactttttt cttcgccgca cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg
     1021 ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc tttcggcttc gtttttttct
     1081 caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgacctga cgacgcttgc
     1141 cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc
     1201 gcggtgcgcg ttcactggag atcagactaa agtcgatttt gcgctcgtcc atattaaccg
     1261 cttcgacgcg aacttccacg cgatcgccca ggcgataagt ctggccgctg gattccccca
     1321 tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa gagacatgga
     1381 ccagaccatc aatgaacaag tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg
     1441 aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac ttcagccagt
     1501 cagcgacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag tgctgaccca
     1561 gttgcaacat ctcttccatc gaataatggt agccgccggt ttcagtggtg ttgccctgat
     1621 gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg tcaggataac
     1681 gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc cagaccgaag tgaccacggt
     1741 tttctggatc gtaaatcgcc tgtttcatcg agcgtagcag catggtttgc agcatttctg
     1801 catcaggacg gtcggcaacc gactccagca gttccgcgta gtcacgcggt tccggcttgt
     1861 taccacccgg cagctccagc cccagctccg ccagcactga acggaaagag gtaatcgctt
     1921 cggtgctcgg cttgtcgtga atacggaaca gtgccggttc tttcgctttc tcaacgaaac
     1981 gcgccgccga gatattcgcc agaatcatgc actcttcaat taatttgtgc gcgtcgttac
     2041 gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt
     2101 cgctctcaaa tgagatccca ccgcgttctt cacgggcttt atccagcact ttatagaggt
     2161 tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg ctcgcgcaga tcctgatcgc
     2221 cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc atcaccgctt
     2281 cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg cacaccatac
     2341 acaggcggtc tacctgcggg ttgagcgaac acaggccgtt agagagcact tccggcagca
     2401 tcgggataac ctgcgaaggg aagtacaccg acgtgccacg gttacgcgct tctctgtcca
     2461 gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat aaacgccagc
     2521 cgccgccgcg ttttttctcg cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat
     2581 caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct tcttccggca
     2641 cttcttcttt cagccccgca acctgttgct caacagcctg cggccagata tacggaattt
     2701 catgggtacg cagagcgata tcaaccgcca tgccggtgcc catattgtcg cccagcactt
     2761 cgacgatttt acccaccgct ttggtgcggc gagtcggacg ctgagtcagt tcgactacga
     2821 ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata tcgaagctca
     2881 gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag cgaccaacaa
     2941 tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga cctttacggt
     3001 cagcacccag cggctgcgcc agcacctgat cgccatgaat gcaggttttc atctgctcgc
     3061 tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag ccatcacggt
     3121 gaccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag cactgacgac
     3181 gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa
     3241 gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg gccggttttt
     3301 cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc gcgtattttt
     3361 cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg tcatctctga
     3421 tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct cttcaaccaa
     3481 atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct tagaaagcgc
     3541 ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga
     3601 taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg gtttacccag
     3661 gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac taagttgatt
     3721 gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt cagaaatact
     3781 ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc cgtaatcagt
     3841 gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata catttatatt
     3901 gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga aggaaaaaag
     3961 cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca atgtatcaca
     4021 ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt cgaacgggtc
     4081 gcgcagaatc atggtttcag tacgatccgg accggtagag atgatatcga tcggcacacc
     4141 ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcaggc cgctacgatc
     4201 tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg gctctacacc
     4261 tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg gcatacggta
     4321 agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag tcaggcagaa
     4381 gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca gccagccggt
     4441 acgacgacga cgacccgtag ttgcaccgaa ttcgttaccc tgcttgcaga ggaactcgcc
     4501 agtttcatca aacagttcgg ccgggaaagg acctgcacct acacgagttg agtaagcttt
     4561 gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg tcgccacgcc
     4621 accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga tatccagcag
     4681 cgtaccctgc gcaccttcga acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag
     4741 gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg tatcatccag
     4801 aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga agttgtgata
     4861 ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg
     4921 cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac cggtggtgcc
     4981 gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agagcaacgt gataatcaag
     5041 gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc cacggtcttc
     5101 cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt taccgatgat
     5161 gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg ttttttcacc
     5221 gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa catatttagc
     5281 ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt gggtgcccag
     5341 tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg gattctacca
     5401 tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc agtctgtttt
     5461 ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca accacaagtc
     5521 caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag atcatcttct
     5581 tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc agggcaagcg
     5641 ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca ccgacatccc
     5701 tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc gttgcggaag
     5761 tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc acgtcctgat
     5821 tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag aagtccggat
     5881 ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca ccacgcataa
     5941 tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg gcagtcgcgc
     6001 gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc gcttcacgct
     6061 cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg atctgcttga
     6121 tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg atgaccggaa
     6181 ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg ttatctgctg
     6241 ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg tcacgtactt
     6301 cgagggtcag acgaccacgg gaatcggtga cgatatcttt cacgtccagg cgaccaattt
     6361 cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc
     6421 caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg atgtaagagt
     6481 cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg tccatggtct
     6541 gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa tgcagaccag
     6601 gctcataaac caaaggtttg ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa
     6661 taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc agcacgatga
     6721 tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt agtcgttacg
     6781 ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg acgtgttgct
     6841 tgctccactg gttgtggagg aagaggctgg cggcagacgc agcagattgc tggcaccgtt
     6901 atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg gcagaaccat
     6961 caggttgcca cccttatcgt taaccagcac tttgcgggtg ttacccaaca ctttttccat
     7021 cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt ccggcagaag
     7081 tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct tgtacgcacg
     7141 cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt cgttggtata
     7201 cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg catcgtcaaa
     7261 cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca gcagcgtgat
     7321 acccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgagtatcgc tacgaatcac
     7381 ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataactc cacgcagggc
     7441 gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca gatatttttc
     7501 cggattggtg acgcggtact gcacgttcat ctcaacgcgc actacgttct cgtccgacgt
     7561 cagcatcaca ccagaagcgg ccagttcacg cacggcttcc acgttcaccg gtttgacttc
     7621 gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga atttaccaaa
     7681 gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg ccgcccagat
     7741 aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc
     7801 ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac caccgagctt
     7861 tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc gaccgccttt
     7921 gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt cgcggtcttg
     7981 tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt atgcggtgat
     8041 ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta cgccgttaga
     8101 tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca acgatcggca
     8161 tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct attgcctgaa
     8221 gctgataaaa acgactcctc agacgccctt cctgcggtgg cagacgcaat gtatgctgcg
     8281 ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg
     8341 tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca atacgcggtt
     8401 cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc tcgtgagcgt
     8461 cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc acatccgccg
     8521 catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg
     8581 ccgccaccag atcgtgcggc aggtggcgaa taaaccctac ggtatctgca agtacggttt
     8641 caccgacatc tgcaacgtca atacgccgca acgtcgggtc gagggtggca aacaactggt
     8701 ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta ccggcgttgg
     8761 tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc cgcccctgct
     8821 cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga ttacgcaaca
     8881 aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc ccgcctttct
     8941 gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca
     9001 gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa
     9061 ggccggtgcg gtcgataaca cgacactcgc acaaacgctc caggttacgc tcttgcgccg
     9121 ggctcagggc atggtcaaaa agaacgaccg aagcacccat cgctttgaca gcttccgcaa
     9181 tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta cggctaccgg
     9241 taatcacctg caatgcttcg acaccggcgg aagagaccag agattcaaac tcctggaggt
     9301 cttccatatc tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc tcaccagcat
     9361 cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg caggatcgct
     9421 ggctccccgt gtaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg
     9481 cggaagtatt ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt
     9541 tgttactgtg atgagaaacc gggcgagatg ggacaacagt agaaatcgcg tgcttgtaaa
     9601 ccatctggct gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc
     9661 cttgcagctt aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg
     9721 cgttcaggaa cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc
     9781 ttatttgtac tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta
     9841 cacattcagc ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ctgttctggt
     9901 ttttcactgt caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc
     9961 gccaactgtc tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct
    10021 tcaaggtaag accacatctg gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc
    10081 aaatctcctc gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga
    10141 tgaaaacgct gctcaatgcg ttgatggagc agttcacggc tcgccggggc gatggcgaac
    10201 tgatgcacct gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta
    10261 cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc
    10321 cttgctgccg caaccggatc tacctcctga agttgacgat gcaatgactc ccaaccttgc
    10381 tctgccgcct gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac
    10441 aacccttcca gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc
    10501 cccgccgcgg tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag
    10561 taagcctgcg acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca
    10621 gcgttcggct tcgccgtccc gataaccatc cctttgtaaa taagggcaga atcaacgctt
    10681 atcaactcta ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc
    10741 gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt
    10801 cagggctttt atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt
    10861 cacaagttgc ggacataacc gttccacgtc cgccagcagg gttatggcct gtgccattga
    10921 ccactgcgca tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc
    10981 gaatacggac tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg
    11041 ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc tgtgcatctg actggaaatc
    11101 aatacccaat tccgctaggg cagactgcgc tttttctaat gccgattttt cttcgccaga
    11161 aacttttagc cgcaacggaa tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt
    11221 caattgtgcc tgacgcagcc aacgttctgc cactggcaag gataaaagtg aaatgttgcc
    11281 atcgcgctcc agcaacgcac agtcggaatg gacgatagtc agtacccgac caaaactctg
    11341 actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct tttggttttt gcatcggcgc
    11401 gggcgtttgc aaaagctggc gatacacttc accttgctgt ttctggtagc ctggctgcgc
    11461 attcggccag ggggcagccg gacgactgcc tgaggcgggc gcaggagagt agcgcggagc
    11521 taccggctca cgagctgccg gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc
    11581 cgggatggca cgcggtgcgg gttgtggttc atcgtccagc ggtagcggcg tttccagttg
    11641 ctgttgtagc acgctcagca cgccctgata gataaaatca tgcaccagac gcgactgatg
    11701 gaaacgcact tcgtgtttgg cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc
    11761 cagatacaac acaaatgccg gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg
    11821 gatcgcatga ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat
    11881 ttccgccagt gcgggcgtag tgtgatttgg atcggccacc cagccgcgca gggtgagatc
    11941 gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcgcc
    12001 taagcgccgt tctttttgcc cgccttccgg cactgcgcgg tactgacgca caattttacc
    12061 gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatttc
    12121 atcaatatgg ctaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt
    12181 gtagaacaga tccagcacct ccagcgtcgt tcccaccgga tgcgctgccg gtttaaccgt
    12241 cacgtccatg tcgcgtcctt cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg
    12301 tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc
    12361 caggctgata atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgagc
    12421 cagcgccagc gccagctcat cttttttgat accgcagccg ttatcacgaa tgcggataag
    12481 tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt
    12541 ttccactagt tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg
    12601 gttcgccagt tgtggcggta agacctgaat tggcatcaat cgtccttagt ttggcagcgt
    12661 gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg
    12721 catcggatgc gcaaggaagt aattgcacag gcctttgtaa atggcttctg ccagctgttg
    12781 ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc
    12841 gaccagtact gatgggatat ccggcgagcg cagaacgcca aggctggcgt gttctggtcg
    12901 acgtttatgt atttcgccaa tgcgttgcaa ctgactgatc atactggtcg ctacatcata
    12961 ccctacccgc tgggaatgac cgaactgtaa atccagcacc gcctggctta aataggggtc
    13021 agactgactg ttcgccagca catcacccgc cccacccagc agctccgaca gtttctcgta
    13081 ctgctccagc cagctggcca tttcactgtt ggcgcgacgg ttagagagca cccatacgga
    13141 agcgccagtc gcactacggt tcggtgcggc atcagcgtga atcgacacga ggaaattggc
    13201 gttttgctta tgtgccacat cactgcgccc catcaccgag ataaagtaat ccccgtcacg
    13261 ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat
    13321 ggcgatggtg acatatttct cccgcgtacc accggggccg atagcgccag ggtcctgacc
    13381 gccgtgtccg gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc
    13441 tgccggacgc gttaccgtat tactgctgat aacacccgta gtgcggttac tttccgtttt
    13501 aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg
    13561 tttcgcaacc acgggcggcg gaggaggcgg tggcggcaca tcggcgttaa tcgtaaagac
    13621 gacagtgtaa ttgctgccat tctgccgctt caccgcttcg gttttaccgt tttcggtaag
    13681 atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt ccagagcgaa tcgccttcac
    13741 cagattattg ccgctgaaca acaacggcag tccctgaatc acgcccgttt gtttgatatc
    13801 gagcgccacg gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact
    13861 caacgttatc cgcgcctgtt gattaccgtt agaaacctga atatcagaga gcgtcgcggc
    13921 acccaccggc gtgcacagca gcagcagcgt cgctaccaac caatttctga tgcgatacat
    13981 catcccgcca cctttcaaag gttaaccggc taaacgcgcc agcaacaatt cacccgcaga
    14041 ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac
    14101 atccgggtca ggaagaacac ctgtaccttg ttgtggccac tccaccaggc agatggcatc
    14161 gttggcaaaa taatcgcgga tccccataaa ctccagctcc tcgggatcgg caaggcggta
    14221 caaatcaaag tgatagacca ttaagttgtc gagcgtatag ggttcgacca gcgtataagt
    14281 ggggcttttg acattaccct gatgacccag agcctgtaaa aagccccggc taaaggtggt
    14341 tttacctgcg cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc
    14401 tacccgctcg cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat
    14461 catggttttt atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg
    14521 ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg
    14581 cgacacagcc tgcacaggct gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa
    14641 taataccaga gagcacatcg cccatgccgc cgctcgccat gcctgcattt ccggcatcaa
    14701 taatgcctaa agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta
    14761 cgccgccata acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag
    14821 cgacggaaca gcctaacaac cgtgcggcct cgctaggatg cggcgtaatc acgcgattgt
    14881 gacgcttatc gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt
    14941 tgcgaaaatt ctcaactttt tgcagggctt ttttccccca ctcttgctgg cccagaccgg
    15001 gaccaatcac caccacatcg gcccattcca ggctttcggt aagagagtcc atagtcagtt
    15061 catgcaccat caattctggt cgtgcagtca gcagcggcgc aatgttctca ctgcgggtca
    15121 gtactcggac taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc
    15181 ccgccgtgcc gtgatcgcca ccgataatca ccagccgccc gtgatcgcct ttatgcgaag
    15241 tcgggcgacg cggtttcagc cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg
    15301 tctcctgacc tgccagccag ctatccagcc ccagtgagtc aaaatgcagt tgtcctgtaa
    15361 catcccgcgc ttttccagtg agcaagccta gtttcagcgc aataaaagtg atggtgtgat
    15421 cggcgttgat cactgcgcct ggcgtagcgc cggtttcagc cagcaggccg gaagggatat
    15481 caaccgccac aatcggcgca ggatgggaat tagcgtggtc gattaactgg ctaatggatt
    15541 cgcggggcgc ttgctgcaag ccggtaccga gcagcgcatc aacaatcaga tctaccgatt
    15601 cgggccagac aatattcgaa gcatggatct cgccacccgc gtttaaccat gcttcgcgtg
    15661 ccagcgcggc ctcttccggc aacggtttgt cgctctcctg ggccaacaac gtgacctcaa
    15721 tgccgaccgc tttggccagt cgcgcgacca cgtagccatc gccgccgtta ttaccatgac
    15781 cgcacagcac cagccagtgg cgggcgtcag gatacgccga acgacacacc tggaatgcgg
    15841 cctcgccagc gcgaagcatc agctcataga gtgtgagccc cagcacatct gccgcctcgc
    15901 gttctccgcg gcggatatcg tcggcgtacc agacggtgtg tggtatactt acggggtttt
    15961 tcttcattgt atggtccgtc atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa
    16021 ttaaacagtg ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca
    16081 gcgagtccga gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact
    16141 ggatggcacg tcacggtatg ctgcgcgctc gcccccatga gttattaccc ggtacgctgc
    16201 gtgtaatcag cgtgcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga
    16261 aaaaccccaa actcggctat gttagccgtt atgcgctggg ccgtgactat cacaaacttc
    16321 tgcgcaaccg actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt
    16381 ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc gttagctgaa aaagctgggc
    16441 tcggctggac aggtaagcac tcacttatcc tcaatcgcga ggccggttcg ttcttctttt
    16501 taggcgaatt gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg
    16561 gcaaatgcgt ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg
    16621 tcgatgctcg ccgctgtatc tcttatctca ccatcgaact tgaaggggcg ctcccggaag
    16681 agttgcgacc gttaatggga aaccgtattt acggttgcga tgactgccag cttatctgcc
    16741 cgtggaatcg ctattcgcaa ctcactacag aagacgattt cagcccgcgt aagccgctac
    16801 acgcaccgga actcattgag ttatttgcct ggagcgaaga gaagttttta aaagtcacgg
    16861 aaggatcggc gattcgccgt ataggtcacc tgcgctggct gcgtaatatc gccgtggcat
    16921 taggcaatgc cccttgggat gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc
    16981 acccacttct cgatgagcac atagcgtggg cgattgcgca gcaaatagag agacgaaatg
    17041 cgtgcatagt cgaagtgcaa ttgccgaaaa aacagcgtct ggttcgggtg attgaaaaag
    17101 ggttaccgcg tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgcattgca
    17161 attcaagacg aataaaatcg tcaagtgatc gactcgacaa aacggaatct tgatttttaa
    17221 cttatttatc aataggttaa aaaataccat tcaccgtgcg aagtttcttt gaaattaaga
    17281 ttacagcctg aggctgtgga taactctgtt tacaagagtt tgtcagagac aacaaaaacc
    17341 agcaataacg catcgcgtcg ctgtagatat tttattgaga gaagaatttg gagcgggaaa
    17401 cgagactcga actcgcgacc ccgacct
//
LOCUS       DAOFMX010000083        17361 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.083, whole genome shotgun sequence.
ACCESSION   DAOFMX010000083 DAOFMX010000000
VERSION     DAOFMX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.083"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..185
                     /locus_tag="QBA20_003216"
     CDS             <1..185
                     /locus_tag="QBA20_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HDQ6293647.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="QBA20_003217"
     CDS             complement(210..401)
                     /locus_tag="QBA20_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDQ6293648.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="QBA20_003218"
     CDS             complement(506..742)
                     /locus_tag="QBA20_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDQ6293649.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="QBA20_003219"
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="QBA20_003219"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDQ6293650.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="QBA20_003220"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="QBA20_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="QBA20_003221"
                     /pseudo
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="QBA20_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="QBA20_003222"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="QBA20_003222"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDQ6293651.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="QBA20_003223"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="QBA20_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDQ6293652.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="QBA20_003224"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="QBA20_003224"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDQ6293653.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="QBA20_003225"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="QBA20_003225"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDQ6293654.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="QBA20_003226"
     CDS             complement(10860..12233)
                     /locus_tag="QBA20_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6293655.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="QBA20_003227"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="QBA20_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDQ6293656.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="QBA20_003228"
     CDS             complement(13344..13583)
                     /locus_tag="QBA20_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293657.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="QBA20_003229"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="QBA20_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDQ6293658.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="QBA20_003230"
     CDS             13960..14247
                     /locus_tag="QBA20_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDQ6293659.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14917..15060
                     /locus_tag="QBA20_003231"
     CDS             14917..15060
                     /locus_tag="QBA20_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDQ6293660.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15073..15282
                     /gene="cspE"
                     /locus_tag="QBA20_003232"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15073..15282
                     /gene="cspE"
                     /locus_tag="QBA20_003232"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDQ6293661.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15448..16257
                     /gene="rlmA"
                     /locus_tag="QBA20_003233"
     CDS             15448..16257
                     /gene="rlmA"
                     /locus_tag="QBA20_003233"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDQ6293662.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16254..16820)
                     /gene="mntP"
                     /locus_tag="QBA20_003234"
     CDS             complement(16254..16820)
                     /gene="mntP"
                     /locus_tag="QBA20_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDQ6293663.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(17283..>17361)
                     /locus_tag="QBA20_003235"
     CDS             complement(17283..>17361)
                     /locus_tag="QBA20_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDQ6293664.1"
                     /translation="SLSFSKSVALHDKVIGHYLNIKHYQ"
BASE COUNT         4586 a         4188 c         4377 g         4210 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggtcgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcta ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctccgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtac
     1561 aggtcgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctagata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac cgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgatgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagcgg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttattt gcgtaacggg atatcagaaa
    14401 aagatatttt cctcattaat ttattaggat gtttacatca gatttgtgat taagcgtggt
    14461 attatttatt agcgaaacgt ttctctcttg atttttttgc tcactcatca atttttctta
    14521 ttttaaattt acaatccctt tggggattga cttctcttta gggtaattga tagccgttaa
    14581 ctgactgttt tatgagaaaa agtgatataa ctttttattc attgcatagc agaaaatgtg
    14641 atattgcacg cactatgtaa taacttctcc ctctggcctg gaacaactga acttattgaa
    14701 ctatgttaga aactacgcca gtttaagtat ctgcctgaac tggcaaggtt aagcacaatg
    14761 atatatcggt gcgtattccg ttgcataagt gtgcaaaaaa agtggaagac gtatcgagat
    14821 ttgtgcgtct gatcgagaca tgtttaaaaa tggcttgcca taattaacgt tgtatgtgat
    14881 aaaagatttc gggttaaacg aggtacagtt ctgtttatgt gtggcatttt cagtaaagaa
    14941 gtcctgagta aacacgttga cgttgaatac cgcttctctg ccgagcctta tattggtgcc
    15001 tcatgcagta atgtgtcagt tttatctatg ttatgcctgc gggcgaagaa aacaatctaa
    15061 ggaatttttc aaatggcaaa gattaaaggt caggttaagt ggttcaacga gtctaaaggt
    15121 tttggcttca ttactccggc tgatggcagc aaagatgtgt tcgtacactt ctccgctatc
    15181 cagggtaatg gcttcaaaac tctggctgaa ggtcagaacg ttgagttcga aattcaggac
    15241 ggccagaaag gtccggcagc tgttaacgta acagctatct gatcgaatcc actgatctga
    15301 agtgtgaata cgcttcaatc tcgctataaa gcctcgtcga atgcgaggct ttttactatg
    15361 ctttatcttc gctcctggcg ttcggatatt tgcccgccgc gtgattcgcg ttacacttgc
    15421 ggcctttagt atcctgccgg agttgtcatg tcgttttcct gtccactttg ccatcagcct
    15481 ctttcgcgtg aaaaaaacag ctatatctgt ccccaacgac atcagtttga tatggcgaaa
    15541 gaagggtatg tcaatctgct gcccgttcag cataaacggt ctcgtgatcc gggcgacagc
    15601 gcggaaatga tgcaagcacg ccgcgcattc ttagatgccg gacattatca gccgctgcgt
    15661 gatgcaattg tcgcccaact gcgggaacgg cttgatgaaa aggccacggc ggtgctggat
    15721 attggctgtg gtgaagggta ttacacacac gcatttgccg atgcgttgcc cgaaatcacc
    15781 acgtttggcc tggatgtctc gaaggtagcg ataaaagcgg cggcgaaacg ctatccgcag
    15841 gtcactcttt gtgtcgcttc cagccaccgt ttgccgtttt ccgataccag tatggacgcc
    15901 ataatacgta tttacgcgcc gtgtaaagca gaagaattag cacgagtagt gaagcccggc
    15961 ggctgggtca ttactgccac gccgggaccg cgacatttga tggagctgaa ggggctgatt
    16021 tacaatgaag tacatcttca tgcacctcat gcagaacaac tggaaggttt tacattacag
    16081 cagagtgcgg agttgtgtta tccgatgcgt cttcgcggtg atgaagccgt cgcattattg
    16141 cagatgacgc cgtttgcctg gcgtgcgaag ccagaagtct ggcaaacact ggcagcaaaa
    16201 gaagtgttcg actgccagac ggactttaat attcacctct ggcagcgttc ttattaaccg
    16261 tggaagtgcg tccagaggat ctggacgccg atgccgatca gcaccagccc gccgagaatt
    16321 tccgcttttt tcccaataat tgagccgata aagcgaccaa ccatcatccc taatgtcgac
    16381 ataatcaagg ttgcacaacc aatggccaat gcggtcgcga taatgttgac ctgaaggaaa
    16441 gcaagaccaa cacccacagc catggcatcc aggctggtgg caatcgcggt ggttaccagt
    16501 agccagaaac cgtgccgacg gcgcggctct tcatcttcat catctgcgcc acgaaaaccc
    16561 tcaataatca ttcgcccgcc gaggaatatc agcagcacaa acgcaatcca gtgattccat
    16621 tcaaggacaa accggctggc taacatgccc attccccagc cgatcagcgg cgtcagggtt
    16681 tcgacggcac caaaaataag gccggttcgc aatgcttcag aaaatttcgg tttatggagg
    16741 ggggcacctt taccgattga tgcagcaaat gcatccatcg acataccaaa cgcaagaaga
    16801 acagtagcag tgatattcat gacaacgtcc tgaccggggt atccataaaa cacatcatcg
    16861 cccccagcaa acatgcatac atgaacaaca agccgcgatg ggttttcgcg ttcatgcagg
    16921 catagcgatt gatgtgcata tggtctcgcc tgactgaaag tcttcagtcc gtacgtgcca
    16981 cgttttgcaa cgagtatgtt gacacgtaca tttccggaat ctggaaatcg gctactcccc
    17041 aaatgagggc gcaaccttaa catattttga gaatataaaa caactgttgg aatatattat
    17101 ttcattgctt tattgataac gattttcatt tatgtttata tatcaatgaa acgttaaaat
    17161 aaatgacaaa taagggaaga gataatatag cctaagctat gtctgattat taaaaataca
    17221 ctaaacatag ctttggctaa attcataagt tattgagttg atacgagggt aatgactcca
    17281 acttactgat agtgttttat gttcagataa tgcccgatga ccttgtcatg cagcgccacc
    17341 gattttgaga acgacagtga c
//
LOCUS       DAOFMX010000084        17088 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.084, whole genome shotgun sequence.
ACCESSION   DAOFMX010000084 DAOFMX010000000
VERSION     DAOFMX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.084"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..506
                     /locus_tag="QBA20_003236"
     CDS             <1..506
                     /locus_tag="QBA20_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDQ6293665.1"
                     /translation="REKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFT
                     QPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRH
                     NRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQY
                     SRAWTVK"
     gene            complement(507..1667)
                     /gene="ybdL"
                     /locus_tag="QBA20_003237"
     CDS             complement(507..1667)
                     /gene="ybdL"
                     /locus_tag="QBA20_003237"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDQ6293666.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1776..2864
                     /gene="hcxA"
                     /locus_tag="QBA20_003238"
     CDS             1776..2864
                     /gene="hcxA"
                     /locus_tag="QBA20_003238"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDQ6293667.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2874..3071)
                     /locus_tag="QBA20_003239"
     CDS             complement(2874..3071)
                     /locus_tag="QBA20_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDQ6293668.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3253..5358)
                     /gene="cstA"
                     /locus_tag="QBA20_003240"
     CDS             complement(3253..5358)
                     /gene="cstA"
                     /locus_tag="QBA20_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDQ6293669.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5539..5952)
                     /gene="entH"
                     /locus_tag="QBA20_003241"
     CDS             complement(5539..5952)
                     /gene="entH"
                     /locus_tag="QBA20_003241"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDQ6293670.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5955..6701)
                     /gene="entA"
                     /locus_tag="QBA20_003242"
     CDS             complement(5955..6701)
                     /gene="entA"
                     /locus_tag="QBA20_003242"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDQ6293671.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSLGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6701..7558)
                     /gene="entB"
                     /locus_tag="QBA20_003243"
     CDS             complement(6701..7558)
                     /gene="entB"
                     /locus_tag="QBA20_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDQ6293672.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7572..9182)
                     /gene="entE"
                     /locus_tag="QBA20_003244"
     CDS             complement(7572..9182)
                     /gene="entE"
                     /locus_tag="QBA20_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDQ6293673.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLCAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9192..10367)
                     /gene="entC"
                     /locus_tag="QBA20_003245"
                     /pseudo
     CDS             complement(9192..10367)
                     /gene="entC"
                     /locus_tag="QBA20_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10659..11615
                     /gene="fepB"
                     /locus_tag="QBA20_003246"
     CDS             10659..11615
                     /gene="fepB"
                     /locus_tag="QBA20_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDQ6293674.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11619..12851)
                     /gene="entS"
                     /locus_tag="QBA20_003247"
     CDS             complement(11619..12851)
                     /gene="entS"
                     /locus_tag="QBA20_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDQ6293675.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12962..13966
                     /gene="fepD"
                     /locus_tag="QBA20_003248"
     CDS             12962..13966
                     /gene="fepD"
                     /locus_tag="QBA20_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDQ6293676.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13963..14955
                     /gene="fepG"
                     /locus_tag="QBA20_003249"
     CDS             13963..14955
                     /gene="fepG"
                     /locus_tag="QBA20_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDQ6293677.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMCLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14952..15767
                     /gene="fepC"
                     /locus_tag="QBA20_003250"
     CDS             14952..15767
                     /gene="fepC"
                     /locus_tag="QBA20_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDQ6293678.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15764..16897)
                     /gene="fepE"
                     /locus_tag="QBA20_003251"
                     /pseudo
     CDS             complement(15764..16897)
                     /gene="fepE"
                     /locus_tag="QBA20_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3990 a         4865 c         4448 g         3785 t
ORIGIN      
        1 ttcgtgaaaa gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg
       61 attacaaccc aaacaacgtg gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa
      121 tagatggttt tacccaaccc attgtggtca cacatacgga taaaaatgcc atggaaatcg
      181 tggatggttt tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa
      241 aaggttattt gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta
      301 ttcgtcataa tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg
      361 aacttagcca gttgggatgg gatgataata aaattggcaa agagctggga atggacagcg
      421 acgaagtgct gcgactgaag caaattaacg gcctgcaaga gttgtttgcc gaccgtcaat
      481 attcccgcgc ctggacggtt aaatagctaa agctggcgca ggcgttcagc tgctgccagc
      541 aacgtcgatt ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg
      601 caaaacaccg acagcggaat cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac
      661 tcaacatcat ccagggtaga aaccgcgctg taatccacca gcaaaaagta tgtaccttca
      721 cacggtaaaa tctccagccg gctttcattt aaagcattca ccagaatatc gcgcttctgg
      781 cgataaaagt ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc
      841 gccagctgtg ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg
      901 gcgctgatgg gcgctggcgc aacacaataa cccactttcc agccggtcat atgataggtc
      961 ttgccaaatg aagaaaccgc cactgcccgc tcacgcagct gcggatgcgc cagcacactg
     1021 gcatggccct gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc
     1081 tcgtgcccgg cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gacagttgca
     1141 ctggggttat gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca
     1201 aattcctgcc agtcaacgcg aaaatgcggt ggttgcagtg ccatacgctt cactattccc
     1261 ccagaaagcg cgatggcggg ggcgtaactg tcatagctgg gatcaaaaca aatcacttca
     1321 tcgccattgc gcaccagtgc ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt
     1381 acggtgatat cgctatcggc atctggttga tagccataca aacgttccgt tttctgagca
     1441 atcgcctcgc gcaaggcctg cacgccggtc atgggcgcgt attggtttgc cccctgggca
     1501 acgtggtacg ccagccgctc ctgtaaatag cgcggaccat caaaatcagg aaagccttgc
     1561 gacaggttaa tcgcctggtg ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg
     1621 ccaagttgtg gaagtttgct ttgtggaatc agagggttat ttgtcatttt tattgtacct
     1681 gacgaagttg cgtgttgaag gcactataac acgatgccag tatttggcaa tcaagacgtt
     1741 tagatgtcta aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg
     1801 tcgtcggccc ggctaactac ttttcacatc caggaagttt caatcacctg cacaattttt
     1861 tcactgatga acaactttct cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg
     1921 cgcaaaccaa acttccgcca gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg
     1981 gtcattgcag cgaaagcgat gtacagcaac tggcggctga gtccggtgac gatcgcagcg
     2041 tggtgattgg cgtcggcggc ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc
     2101 tcggtctgcc atttgttgcc gctccgacga tcgccgccac ctgcgcagcc tggacaccgc
     2161 tctccgtctg gtataacgat gccggacagg cgctgcatta tgagattttc gacgacgcca
     2221 attttatggt gctggtggaa ccggagatta tcctcaatgc accgcaacaa tatctgctgg
     2281 cggggatcgg tgacacgctg gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag
     2341 aaacgttgcc gctaaccgtg cggctgggga tcaataatgc gcaggccatt cgcgacgtct
     2401 tgttaaacag tagcgaacag gcacttgccg atcagcaaaa tcagcagtta acgcaatcat
     2461 tttgcgatgt ggtggatgct attattgctg gtggtgggat ggtcggtggt ctgggcgatc
     2521 gttttacgcg tgtggcggcg gctcatgccg tgcataacgg tctgactgtc ctgccgcaaa
     2581 ccgagaagtt tcttcacggc accaaagtcg cttacggaat tctggtgcaa agcgccttgc
     2641 tgggtcagga tgatgtgctg gcgcaattaa ccggggcgta tcagcgtttt catctgccga
     2701 ctacgctggc ggagctggag gtggatatca ataatcaggc ggagatcgac aaagtgattg
     2761 cccacacctt gcgcccggtg gagtccattc attacctgcc agtcacgctg acaccagata
     2821 cgttgcgtgc agcgttcgaa aaagtggaat cgtttaaagc ctgacgtgca aatttagcag
     2881 cagcgcgcgc cgccttttcc accgtagcgc gcgtcctgcc gctcacggaa aaactcctcg
     2941 taggtcatcg gcgtttgatc gggatgatta acccgcatat gttcgacata gttgtcgtaa
     3001 tcaggcatac caatcatcag cttcgccgcc tgacctaaat attttccggc ttttgccagt
     3061 gaatcaaaca tcttggcctc cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga
     3121 acgctttatc cggcctacaa atgctatcaa cgcactcggt cagtcccctc gccccatcgg
     3181 ggagagggtt agggtgaggg ggaaccctcg gcactatgcc aacattcgcc aacatccccc
     3241 ctcactctga ctttagtgcg cgcctttcgc ctgcgctacg atctcctcga cattttccgg
     3301 catcggctca tacggcgttt ctttcgccgt tggcttcgga tctttcaatg ccgcaagtgc
     3361 cgtcttaatc gagaacagtg ccaggaccac cacgaccacc ataaagaaga tggttaaccc
     3421 ggcatccaga cggttgttga acaccagttg cgccagctgt gactcagtat actgcgacgg
     3481 aatattgccg ctgtcgatca ttgcctggaa cttgttagca atggccagga agccgacttt
     3541 cgcatccggg ctaaacgctt tctgccagtc agcggtcagg gtacaaatca gcagccaggc
     3601 cgttggtacc agcgccaccc aggcgtaacg ttgacgtttc atcttgaaca acaccacggc
     3661 acagagcatc agcgccatcc ctgccagcat ctggttggca ataccaaaca gcggccacag
     3721 agtgttaata ccgcccagcg gatcgaccac gctctgatgg aggaagtagc cccacgccag
     3781 tacgcacagc gctgttgcca gcaggttagc aggcagtgaa tcggtccgct tcaggccagg
     3841 agacaccacg cccagcagat cctgcaacat aaagcgcgca gcacgcgtac ccgcatctac
     3901 cgccgtcaga ataaacagag cttcaaacag aatggcgaag tgataccaga acgccacatc
     3961 catcatacca cccagcgcac cgtgcaggat gtaagccatc cccaccgcca acgtcggcgc
     4021 accgcccgca cgggaaatga tcgactgttc acccacttcg ctggcaatct ggttgagcgt
     4081 atctggtgta atgctaaagc cccagctact caccacctgc gcggcagaag cgactacatc
     4141 cgccgtccct gccggagcca gcaccgccat tgggctgttc atggcaaaat acacgcccgg
     4201 atcgatgata caggcagaaa ctagcgccat aatcgccacg aaggattcca tcaacatccc
     4261 accgtagccg ataaagcacg cctgcccttc gttcgccagc atcttcggcg tggtgccgga
     4321 agagatcagc gcatggaagc cagacaccgc accacaggcg atggtgataa acaggaacgg
     4381 gaacaggtta ccggtccata ccgggccagt gccatcgaca aatttggtca gcgcaggcat
     4441 ggtcagcgtc gggcgcataa tcaaaatgcc taccgccaga ccgacgatag tcccgatttt
     4501 caggaaggtc gagagatagt cacgcggggc cagcagcaac cacaccggca gcaccgccgc
     4561 cacaaaaccg tagcccacca gcatccaggt cagctgcacg ccggtaaagt caaagtacgg
     4621 tgcccaggtc ggactttctg ccacccagcc gccagagata atggcgaaaa tcaggaacac
     4681 caggccaatc actgacactt cgccaatgcg gcccggacgc agatagcgca ggtagattcc
     4741 cataaaaatc gccagcggaa tggtgaacgc aacggtatac gttccccacg ggctatgagt
     4801 cagggctttc accacgatca tcgccagtac tgcaaggata atgaccatga tcataaagca
     4861 ggctaccagc gcaatcaccc cggcagttgg ccccatctct tctttgacca gctcacccag
     4921 cgagcgaccg tcacggcgcg tagaaacaaa cagcaccatg aaatcctgca ccgcaccggc
     4981 gagaaccacc ccggcgagca gccagatcat ccccggcagg tagcccattt gcgctgccag
     5041 caccggcccc accagcggcc ctgctccggc aatggccgca aaatggtgac cgaacagcac
     5101 tttcttgtcc gtcggcacat agtccagccc gtcgttatgg cgcaccgctg gcgtcatacg
     5161 cgtcgggtca accgccagca catttttggc gatatacagc ccataaaaac ggtaagcgat
     5221 cagatagata cagaccgacg ccaccacaat ccacagcgcg ttgatctgtt ccccacgatt
     5281 taaagcaatg tatcccagag caaatgctcc cattacagag agcactgtcc agacgaggta
     5341 tttccctgat ttgttcatag ttgttatccg tgtgcgtgtc catagagatg ttacattttg
     5401 tttctataac atctttgtac gatttaacaa cacggtaacc atgtaaatcg gagttgaaac
     5461 caatatttaa cttaacaatg ttaactcgat cactccgtta accgagtttt cctgcaatct
     5521 catttaattc tgtcgggctc atcccaaaac tgccgtaccc agccgacaag tgcagcaacg
     5581 ccgcccctgt tcatcgaaaa cgacgatttc ccagctctga ttttgccgcc caagatgcag
     5641 cggctggcag acgccgcgta ccttgccttc agacaccggg cgatgatggg tggcgttaag
     5701 ttccgtgccc accacacact gcccgtcacg ggtcatcata aatccggcca tcgatcccag
     5761 cgtttcagcc agcgccgccg acgcgccgcc atgcagcagg ccaaacggct gatgagtacg
     5821 ggtatcaacc ggcatttcgg cttccagcac atcatctccc agacgggtat acacaattcc
     5881 cagatgcgcc accattgtgt tatcgctggt ggcgttcagt tcgtcgagcg ttaaatggcg
     5941 tttccagatc atgcttatgc ccccagcgtt gagccgccat cgaccacaat atcctgtagg
     6001 gtgatatggc tggcgaggtc agaggcgagg aacaaaatcg tgttggcgat ctcttgtgga
     6061 cgggcgattt tccccagcgg aatgccgagt ttaaactgct cgccaaagcc gcgaatacgc
     6121 tgttcttcgg cgtcatcgct cacccacagc gtgcgttgca tatcggtgtc ggtggagcca
     6181 agcgaaacca cattacagcg cacgccgcta cccgccagtt ccagcccgac gctcaacgcc
     6241 aggcttttca gcgccgcttt cgatgcgcca taagcactca taccaatacg cgccgtgtgt
     6301 gcggcgtcgg acgccacagt gacaatcgcc ccgccccgct gacggcgaaa ctggttcatg
     6361 gtttgctgga acaggttaaa cgcaccgccg acgttaaccg caaaagtctg ctgccagtcc
     6421 tctttactga gctgatcggt cgcgcccatg cgcaaaattc ccgccgcatt gatcagcacg
     6481 tccagtcgct ccgtttcagc taacagtcgc tgacatacct gcgccacctg cccggcgtcg
     6541 gcaacatcca ttacttcggt cgcaaaggga tattgctcct gagtgaacgc ttgatcaaaa
     6601 cctgtaactt tcgctcccgc ctcaacaaac gccagcgccg tggcgtagcc gataccttta
     6661 cctgcgccgg ttacccagac atttttaccg ctgaaatcca ttatttcacc tcgcgggaga
     6721 gtagcttcca ccaggcgtcg atggtcgggt ttttcgccag catgacaaag tcgatatcac
     6781 catgcacttt gcgccagcgc gccgccagcg ccatcatgcg caccgaatcc agaccgtagt
     6841 cgatcaggtt gtcgtcatcg aacggttcat cggactcgtc cagcaacggc aggatcacct
     6901 cacgcagcgc cgctttgctg gcggggatag gtgctggcag taattcttcg gtcatcacca
     6961 cccggccaga acgtccggcc acatatttca gcgacatcaa atgctcgtca cggctgaaat
     7021 cagccagcgc atccgccacc ataaacggtt taatatcgcg cataaatgcg tcggttgcgg
     7081 tggtcataca gccaatgtgg gcatatactc ctgtaataat cagctggtta cgtccactct
     7141 ctttgagcat ttgctccagc ggagaacgat gaaacgcgct gtagcgccac ttcaccagca
     7201 ccgtgtcgtc ggcatctggc gtcaggcgat ccaccacctt ttgctgttcc ggcgagcggg
     7261 tcaggcccgg cccccacata tcattcaaca gcgcccgatc ttcatcgctc tgctctttcg
     7321 gctgggcggt gtaataaacc gggatattgt gctgtttgca gtagtcgcgc agcgcagcaa
     7381 tattcgcgat cacctgctcc atcatcgggc agttctcgcc ccagaagctg acaaaatagt
     7441 cctgcatatc atggattaac aacgcggcac gttgcggttc aaaggcccag tcaactttat
     7501 tctgcggaat atcgtgagac tccggcagtg cgtaagcctg taattttgga atagccatcg
     7561 tgttctctcc ttcaggctga tgcgcgtgac gccagccact gacgtaattg ttttttatcg
     7621 actttcccga ccgccgtcag aggaagtgaa tccacacact ccacgcgatc cggtaattta
     7681 aattcggcaa taccctgttc acgcaggaaa cgacgcacct gcaccgcgca cagcggctct
     7741 tttaccacca gataagcaca gcttttttcg cccatcagct cattttccat gctcaccagt
     7801 gcggcataga tcactgccgg atggctcagc agcaggtttt cgatatcttc ggcagcgatc
     7861 ttctcgccgc cacggttgat ctgatctttc tcgcgcccct gcacggtgat gtacccctct
     7921 ggatcaatag agatcagatc gccggaacag taaaaaccgt tggcatcaaa ggcactggca
     7981 ttgtgctgcg gacttttgta atagccacgg aaggtgtacg gcccgcgcgt catcaggcgt
     8041 ccaacttccc cttgcggcag tggatttcct tcggcatcgg caacccacac ttcatcgtcc
     8101 ggacacattg ggtaaccctg ggtatggata attttctccg cgctatcatc aagacgggtg
     8161 tagttcacca gcccttccgc catgccaaac acctgctgca actggcagcc aatctcagcg
     8221 ggaatacgcg ctgcaagcgt ggcagaaaga cgtgcgccgc cgacctgtaa cagtttcagc
     8281 gaggcaagct gcgcccggct ttcgccttca gccagcgcct gcaaccacag gctgactgcc
     8341 ggcggcacca gcgcggtaac gttaacctga tgttttgcaa tcaacgggaa gcaaagcgtg
     8401 gcgctgggat cggccgccag aacaaccgtt cctccggcaa gaaagacgcc cagcgatcct
     8461 ggcgaactca tagcgtagtt atgagccgcc gggatcgcgc acaggtagcg cgtctgttgt
     8521 gtgaactgac aaatctcggc gctacgacgc acgctgtagt aatagtcgtt atgagtgcgc
     8581 gggatcagtt tcggtgtgcc ggtggtgccg ccggaaagct ggaaataggc cacttcatca
     8641 gcaggtgatg gcgtggcagt aaaaccctct gccggatggt taatcgcatc ctgcaagtta
     8701 tgctcaccgc tgtcgttgtg cagttgcacc acgcgaatgg aagaatgctc tgcaacaaat
     8761 gtgttgagga aatcatcccc gctaaatagc gcatgctggc gatcggcaat cagtaacgcc
     8821 ggttcaatct ggctggcgta ggcgttcagt tcactacgct gatggctgaa cagcgccagc
     8881 accggcgcga cgcccagttt cagcagcgcg aaaaaggtaa tgtaaagttc agcgacgtta
     8941 cccagttgta ccagcgcggt ttcaccaggt ttaatgccct gacggcgcaa actacaggcg
     9001 aggttatccg ccgcctgatt cagctcccga taactcaact gccgctcgcc gtcgataacc
     9061 gcgatgctgt cactcgcagc gtggcgagtc agaatgtcgg tcagtggcaa atcctgccag
     9121 tagccttttt cccgatagcg acgggcaaac tcttccggcc agcgggtgaa tggaatgctc
     9181 atcctcgctc cttaatgcaa tccaaaaacg ttcaacatgg tagaaagttt gacgcctgtt
     9241 tcgcgccact cacccaacgg tgacgacgca ggcacaatcc ccgctccggc aaacagacgc
     9301 acctgatttt cccgcagctt cgcgcagcgg atggtcacca cccattcgcc gttaccttcg
     9361 ctgtcacacc aacccacaat gccgccaaac agttcgcggt cgaacggctc cagttcagca
     9421 ataacctggg tcgcggcctg atgcgggaaa ccgctcagcg cgggggtcgg atgcagcaga
     9481 caggccagag tcagtgcgtt ttcttgcgaa ttcgctttac cttcaaaggg agttgcgaga
     9541 tgccacagcg tcggcgtggt aatcaattgt ggagaggagg gaacgtgtaa ctcactactg
     9601 cgttcgcgca gtacctcttt catcgcctga gtcaccagtt catgttcatg gcgatctttt
     9661 tctgacgcca gcagacgatt acccgcttcg cgatcgagca cttcatccgg ctgacgacgc
     9721 gcggaaccgg ctaacggaat ggagctaaaa cgctcgccgt ctttacgtag cagcagttcc
     9781 gggctggccc ccagcaggac gccaccatca gccagcggga catggaagtt gtaactaacc
     9841 gggttttgcg caatcaaccg ttccagcaat acgccactat caatggcggc gtcagtggtg
     9901 atatcaatca atcgtgacaa caccactttg tcgacctgcg gcgtggcggt aagtgcggcg
     9961 gcgcgggcac ccatctgttc aaacgtggtt tgttcaggaa ttgcctggcg ttccaccacg
    10021 ttcagcgact ggctgcgggt aaaacagcgg gctgaggtct gtttttcctg acgggagaac
    10081 gactgctagg attcgggaat atacagcgac gaaggctgac gtggatcgaa gggaatcgcc
    10141 ccgaccatca ccggattttt gatgccctgc gctttggcat cggcaaacag cgcggcgagt
    10201 ttttgctgga agggactgtc gggcgaatcc ccgttcacag ccggttcatc gaaacgggcg
    10261 aaacatcctg acgtcgtaaa actgcggtac ggcgacataa agaaaaagcg attgggcgca
    10321 agtgttgcca tggtctgctg tacttcctca gccagtgacg tatccatatc atcctccaca
    10381 aaatgataaa ggctttaata atgattatca cttatatttt cggccgctaa cctaaaagca
    10441 aacgcgcact atgtcaactc ttgaggtaac gcaatctgca actcggggtt gcatctggtt
    10501 gtggttacaa tgaaaatgag aagcattatt gatgaattcg cataagcgca atgtgatgtc
    10561 ctgcgccgtt ttgccccctc tccccaaagg gacggattgt gcgctttgtc gaatttgtca
    10621 ttacgccctt aaccttatta ataacaggaa gctgacttgt gagactcgcc ccgctctacc
    10681 gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgtagtt caggccgccg
    10741 actggccgcg tcagattact gacagccggg gcactcatac cctggaaagc cagccgcagc
    10801 gtattgtttc caccagcgtc accctgaccg gatcactgct ggcgattgat gctccggtga
    10861 tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcaga tgaccagggc tttttacgcc
    10921 agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg
    10981 ccgaagccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggggatt
    11041 cggcgctggc gctgtatgat cagctttcca ccatcgcccc gacattaatc atcaattacg
    11101 acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg catgagaaac
    11161 aagcggcaga gcggattgca cagtttgata agcaactggc agcggcgaaa gagcaaatca
    11221 aattaccgcc gcagccggtc actgccattg tctataccgc cgctgcacac agtgccaatc
    11281 tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga
    11341 aattgcccgc agggttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc
    11401 ttggtgggga aaatctggct gcggggttaa acggtgaatc gctattcctg ttcgctggtg
    11461 atcagaaaga tgccgatgcg atttatgcca atccactgct cgcgcacctg cctgcggtac
    11521 aaaacaagca ggtttatgcg ctgggaaccg agacgttccg cctggattac tacagcgcca
    11581 tgcaagtgct ggaacggctt aaggcgctgt tttaagcatt aactgtcgga cgctgtcacc
    11641 tgcggcggcg tctggcgaaa acgtcgcaac tccaccagca ccagcaataa caacacgccg
    11701 ataatcaaca aaccaaaacc gctcgcgctt gcggaagcaa ccggtgtcat catcgcgccc
    11761 aaaccaccca gcagcgccgc gcctatggca tcgcccgtca cgttctgcgc cgtccacaaa
    11821 ccgttaatcc gccctaacat cgcttccggg gtttgcgttt gcagcattgt atattgcagc
    11881 aacgaactaa ccgcgctcag ccagccgaac agcgccagac aaaccacgcc taaaatccac
    11941 atcggcatca ggccaaacag accaatggcg aggaacgatc ccagcgtgga gagcagcatc
    12001 aataaccctg gtcgcgcact atgtgccagc ttcccgctgg ttaacgcacc aatagccgcg
    12061 ccgagcggga tcgccgcgta gagaaaacca atctgtgccg ctgacatctg ccagttgtca
    12121 gccagcgccg gatacagtac cagcaccgcg ctcgccatcg tcaataaacc acccagcagc
    12181 gggctggcaa gcagaaaacg aaatcctgcc agtaatgatt tcaacggatg ctcaagcggc
    12241 tgcggtggcg gtggcaacgc cggaaggctt aataacggta acaaggtaat aaacgtgccc
    12301 gccgccgcca gcccgtagtt ccaggctacg ccgccggtcg ccagcaataa accgccaatc
    12361 atgggcgaaa tcaccgaccc caggcgcacg gtcaacatgg tgatcgcccc ggcctgcatt
    12421 aagttttcac gccctaccag tgcagatgtt gccgccagta gcgccgtaac accaagtgat
    12481 gcgaaaaaac catcccataa accaagtaaa tagattgcca gcaatgacgg ctccggcagc
    12541 agtgcattaa ggcacagtcc aatgaagcca atgccacagg tgccgcgcgc cagcaaaatc
    12601 acttttttgc gttcatagcg atccgccagc acaccgccaa ccatcaggcc aacaaacatc
    12661 gcgccgccgg tcagcgtcac cgaaagcccc acctgccagg tagaatgggt catcatctgg
    12721 atctgcaccg gcaccgcgac gccgagcaaa cccagagaca caattgagat aaaacgagcg
    12781 aggaatactg cgcgaaacgc cgggtgcgtt ttcaacaggc tgaggttaag cagccaggat
    12841 tgtttattca ttacaatgcc ttgccatcaa atattttaaa tacctattct taggctgcac
    12901 atgttaacat atccaaataa gatcgataac gataattaat ttcattatca tggaagttcg
    12961 tatgtctggt tctgttgccg tgacacgcgc cattgccgtg cccggattgc tgttattact
    13021 gattatcgcg acggcattaa gcctgctcat tggggcaaaa tcactccccg cttccgtagt
    13081 gctggaggcc ttctccggca cctgccagag cgccgactgc accatcgtgc tcgacgcgcg
    13141 gctgccgcgt acccttgccg gtttactggc aggcggcgcg cttggccttg ccggggcgtt
    13201 aatgcaaacc ctcacccgaa acccacttgc cgaccccggc ttgcttggcg tgaacgccgg
    13261 agccagcttt gccattgtgc tgggtgcggc gctgtttggt tactcatccg cacaggaaca
    13321 actggcgatg gccttcgccg gggcgctggt ggcctcattg attgttgcct ttaccggcag
    13381 tcagggtgga gggcagttaa gtccggtgcg tttaaccctg gcgggcgtgg cgctggcggc
    13441 ggtgctggaa ggactgacca gcggcatcgc cctgcttaat cctgacgtct acgatcaact
    13501 gcgtttctgg caagccggtt cgctggatat tcgcaatcta cataccttaa aagtggtgct
    13561 gatcccggtt ctgatcgccg gagcaactgc gctattactg agtcgcgcgc tgaacagttt
    13621 gagcctcggc agtgacaccg cgacggcgct gggcagtcgc gtggcgcgca cacagttgat
    13681 tggtctgctg gcgattaccg tgctttgtgg tagtgcgacg gcggtagttg gcccgattgc
    13741 ctttatcggc ctgatgatgc cgcacatggc gcgctggctg gtaggtgccg atcatcgctg
    13801 gtcgctgccc gtcacgctac ttgctacccc tgccctgctg ctgtttgccg atatcatcgg
    13861 gcgggtgatt gtgcccggcg aactgcgcgt ttctgtggtc agtgcgttta ttggtgcacc
    13921 ggtgctgatc ttcctcgtgc gacgtaaaac gcgaggtggt gcatgattta cgtctctcgc
    13981 cgattactca tcacctgttt gctgctgatt tccgcctgtg tggttgcagg tatctgggga
    14041 ttacgcagcg gtgccgtcac gctggaaatc tcgcaggtat tcgccgcgtt gatgggcgat
    14101 gcgccgcgca gtatgacgat ggtggtcacc gaatggcgtt taccacgcgt gctgatggcg
    14161 ctgttgattg gcgcggcgct gggcgtcagc ggcgcgattt ttcagtcgct gatgcgtaac
    14221 ccgctcggca gccctgacgt aatgggcttt aacaccgggg cgtggagcgg cgtgctggtg
    14281 gcgatggtgc tgtttggtca ggatctgacg gctatcgcgc tggcagcaat ggtgggcggc
    14341 attatcactt cgctgttggt ctggctgctc gcctggcgta acggtatcga cacctttcgg
    14401 ttgattatta tcggtatcgg cgttcgcgcc atgctggtgg cctttaatac ctggctgttg
    14461 ctgaaagcgt ctttagaaac ggcgctaaca gcaggtttgt ggaatgccgg atcgctcaac
    14521 ggcctgacgt gggcaaaaac ctcgccttcc gcacccatca ttatattgat gctcattgcc
    14581 gccaccttgc tggtgcggcg aatgtgcttg ctggagatgg gcgatgatac cgcctgcgcg
    14641 ctgggcgtca gcgtcgagcg ttcgcgtctg ttaatgatgc tggttgcagt ggtgcttacc
    14701 gctgcggcaa cggcgcttgc cgggccgatt tcctttattg ctttagtcgc gccgcacatt
    14761 gcccgacgca ttagcggcac cgctcgctgg gggctgaccc aggcggcgct gtgcggagcg
    14821 ctgttactgc tggcggccga tctctgcgcc caacaactgt ttatgccgta tcaacttccg
    14881 gttggcgtcg ttaccgtcag cctcggcggt atttacctta tcgtcttgtt aattcaggag
    14941 tctcgcaaaa aatgaccgaa tcagtagccc gtttgcgcgg cgaacagtta accctgggat
    15001 atggcaaata taccgttgcg gaaaatctga ctgtagaaat tcctgatggt cacttcacgg
    15061 caattatcgg accaaatggc tgtggtaaat ccacgttact gcgtacctta agccgcctga
    15121 tgacgcctgc tcacgggcat gtctggctgg atggcgagca cattcaacat tacgccagta
    15181 aagaagttgc acgccggatt ggtctgttgg cgcaaaatgc taccacgccg ggcgatatca
    15241 ccgtgcagga gctggtggcg cgtggacgtt atccgcatca accgctgttt acccgctggc
    15301 gcaaagagga tgaagaagcg gtaacgaaag cgatgcaggc cacgggaata actcatctgg
    15361 cagatcaaag cgtggatacc ctttctggcg gacaacgcca gcgagcgtgg atcgcgatgg
    15421 tgctggccca ggaaacggca attatgctgc tcgacgaacc cacgacctgg ctggatatca
    15481 gtcatcagat tgatctactg gaactgttaa gcgaactgaa ccgcgagaaa ggctataccc
    15541 tggcggcggt gctgcacgat cttaatcagg cctgtcgtta cgccagccat ttgattgcat
    15601 tgcgggaagg gaaaattgtt gctcagggag cgccgaagga gattgtcact gctgaactga
    15661 ttgagcgcat ttatggtctg cgatgcatga tcattgacga tccggtagcc ggaatgccgc
    15721 ttgtggtgcc gctcggacga acggcacctt caaccgcaaa tagttaaact aagtggtctg
    15781 ccatcatggc atcctgtttt ctggatgcca tcgcatagcg caataacacg ctaccacaag
    15841 ccaccatccc gccgatcagc gcggaaagga tcacaataat cgccttaccc ggaccgtctt
    15901 ttttcactgg caatgacggg cttaactgat atttaaacgg cgtaaaattc acatcgttga
    15961 catttgcttt tgttaattgc tcgacaagat actgccgatt acgtaattca ccgttcagtt
    16021 ccgcaacgtc agtaaccgct ttttcaattt ccagtttgcg ttcaataccg tctgcgccga
    16081 gagaaataga gaaatcgggg tcatctttaa cggcctgacc attactgtat acgggctttt
    16141 taattcctgc cgcgttggca atgtcgagtg aataattgag gcgctgaatg tttgcatcaa
    16201 gttgattttt cgttttaatg cgatcctgag ccagtttttc tttttcaaac tgggttttga
    16261 tctccagttt attacggacg ttttctatcg actctttcac caccaacgca gagatataat
    16321 cgatataccc gctcaaaacg gtctgcgcct ctttactggt tggcgcggta aaacttagcg
    16381 tccaggaggt atacagtgac ggttcatctt ttttcttact agcattgtca tcaaccgctt
    16441 tcattttttc gctcaatgca acaatagcgc gatgcaaatc cagttcatcg attttcgcct
    16501 cttttaatta gtccatcaca taaggtgatg aacgcaggta ctcttccagc aagctaacag
    16561 actgaaactt cttgataaac aggttaaatg caccgccacg gtcgatatta acatcgagat
    16621 caagaacgcg gagtttggta aaggttttct ccaggtcctg ccactgaatg gcttcggcgg
    16681 gagtaataac ggctgagctg gtccattttt gcggcaggat gaaagagatc agtaaacctg
    16741 cgcaggcaaa tgcgaaaacg accgccatga ccgttttttt ggcccgccat aaaacttcga
    16801 ttagattaag taaatcaatt tcattattac tgggcgacgc cagaggatac tcagaaaaat
    16861 gagcgtcact tccctgttta atattcaatg atgacatggg gtataacctg aaaatgagtc
    16921 caatagggga aaatctccac ggcgctcact ttaccagttg gtgagaatta tccgaagctg
    16981 aagtttgtaa attccttcca ctgaatgcaa cattcactct atttaagaga aatattcatc
    17041 aaacctataa atcaaacaaa taaaaaaccc ataattattt tctgtaat
//
LOCUS       DAOFMX010000085        16465 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.085, whole genome shotgun sequence.
ACCESSION   DAOFMX010000085 DAOFMX010000000
VERSION     DAOFMX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.085"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..954
                     /gene="tuf"
                     /locus_tag="QBA20_003252"
     CDS             <1..954
                     /gene="tuf"
                     /locus_tag="QBA20_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDQ6293679.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            1137..1331
                     /gene="bfd"
                     /locus_tag="QBA20_003253"
     CDS             1137..1331
                     /gene="bfd"
                     /locus_tag="QBA20_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDQ6293680.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1404..1880
                     /gene="bfr"
                     /locus_tag="QBA20_003254"
     CDS             1404..1880
                     /gene="bfr"
                     /locus_tag="QBA20_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDQ6293681.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1877..2344)
                     /locus_tag="QBA20_003255"
     CDS             complement(1877..2344)
                     /locus_tag="QBA20_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDQ6293682.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            2725..3036
                     /gene="rpsJ"
                     /locus_tag="QBA20_003256"
     CDS             2725..3036
                     /gene="rpsJ"
                     /locus_tag="QBA20_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDQ6293683.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            3069..3698
                     /gene="rplC"
                     /locus_tag="QBA20_003257"
     CDS             3069..3698
                     /gene="rplC"
                     /locus_tag="QBA20_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDQ6293684.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3709..4314
                     /gene="rplD"
                     /locus_tag="QBA20_003258"
     CDS             3709..4314
                     /gene="rplD"
                     /locus_tag="QBA20_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDQ6293685.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            4311..4613
                     /gene="rplW"
                     /locus_tag="QBA20_003259"
     CDS             4311..4613
                     /gene="rplW"
                     /locus_tag="QBA20_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDQ6293686.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4631..5452
                     /gene="rplB"
                     /locus_tag="QBA20_003260"
     CDS             4631..5452
                     /gene="rplB"
                     /locus_tag="QBA20_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDQ6293687.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5469..5747
                     /gene="rpsS"
                     /locus_tag="QBA20_003261"
     CDS             5469..5747
                     /gene="rpsS"
                     /locus_tag="QBA20_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDQ6293688.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5762..6094
                     /gene="rplV"
                     /locus_tag="QBA20_003262"
     CDS             5762..6094
                     /gene="rplV"
                     /locus_tag="QBA20_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDQ6293689.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKTA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            6112..6813
                     /gene="rpsC"
                     /locus_tag="QBA20_003263"
     CDS             6112..6813
                     /gene="rpsC"
                     /locus_tag="QBA20_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDQ6293690.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6826..7236
                     /gene="rplP"
                     /locus_tag="QBA20_003264"
     CDS             6826..7236
                     /gene="rplP"
                     /locus_tag="QBA20_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDQ6293691.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            7236..7427
                     /gene="rpmC"
                     /locus_tag="QBA20_003265"
     CDS             7236..7427
                     /gene="rpmC"
                     /locus_tag="QBA20_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDQ6293692.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7427..7681
                     /gene="rpsQ"
                     /locus_tag="QBA20_003266"
     CDS             7427..7681
                     /gene="rpsQ"
                     /locus_tag="QBA20_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDQ6293693.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7846..8217
                     /gene="rplN"
                     /locus_tag="QBA20_003267"
     CDS             7846..8217
                     /gene="rplN"
                     /locus_tag="QBA20_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDQ6293694.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            8228..8542
                     /gene="rplX"
                     /locus_tag="QBA20_003268"
     CDS             8228..8542
                     /gene="rplX"
                     /locus_tag="QBA20_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDQ6293695.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8557..9096
                     /gene="rplE"
                     /locus_tag="QBA20_003269"
     CDS             8557..9096
                     /gene="rplE"
                     /locus_tag="QBA20_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDQ6293696.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            9111..9416
                     /gene="rpsN"
                     /locus_tag="QBA20_003270"
     CDS             9111..9416
                     /gene="rpsN"
                     /locus_tag="QBA20_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDQ6293697.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9450..9842
                     /gene="rpsH"
                     /locus_tag="QBA20_003271"
     CDS             9450..9842
                     /gene="rpsH"
                     /locus_tag="QBA20_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDQ6293698.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9855..10388
                     /gene="rplF"
                     /locus_tag="QBA20_003272"
     CDS             9855..10388
                     /gene="rplF"
                     /locus_tag="QBA20_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDQ6293699.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10398..10751
                     /gene="rplR"
                     /locus_tag="QBA20_003273"
     CDS             10398..10751
                     /gene="rplR"
                     /locus_tag="QBA20_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDQ6293700.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10766..11269
                     /gene="rpsE"
                     /locus_tag="QBA20_003274"
     CDS             10766..11269
                     /gene="rpsE"
                     /locus_tag="QBA20_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDQ6293701.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            11273..11452
                     /gene="rpmD"
                     /locus_tag="QBA20_003275"
     CDS             11273..11452
                     /gene="rpmD"
                     /locus_tag="QBA20_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDQ6293702.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11456..11890
                     /gene="rplO"
                     /locus_tag="QBA20_003276"
     CDS             11456..11890
                     /gene="rplO"
                     /locus_tag="QBA20_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDQ6293703.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11898..13229
                     /gene="secY"
                     /locus_tag="QBA20_003277"
     CDS             11898..13229
                     /gene="secY"
                     /locus_tag="QBA20_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDQ6293704.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            13261..13377
                     /gene="rpmJ"
                     /locus_tag="QBA20_003278"
     CDS             13261..13377
                     /gene="rpmJ"
                     /locus_tag="QBA20_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDQ6293705.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13524..13880
                     /gene="rpsM"
                     /locus_tag="QBA20_003279"
     CDS             13524..13880
                     /gene="rpsM"
                     /locus_tag="QBA20_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDQ6293706.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13897..14286
                     /gene="rpsK"
                     /locus_tag="QBA20_003280"
     CDS             13897..14286
                     /gene="rpsK"
                     /locus_tag="QBA20_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDQ6293707.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            14320..14940
                     /gene="rpsD"
                     /locus_tag="QBA20_003281"
     CDS             14320..14940
                     /gene="rpsD"
                     /locus_tag="QBA20_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDQ6293708.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14966..15955
                     /gene="rpoA"
                     /locus_tag="QBA20_003282"
     CDS             14966..15955
                     /gene="rpoA"
                     /locus_tag="QBA20_003282"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDQ6293709.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15996..16379
                     /gene="rplQ"
                     /locus_tag="QBA20_003283"
     CDS             15996..16379
                     /gene="rplQ"
                     /locus_tag="QBA20_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDQ6293710.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4221 a         3877 c         4402 g         3965 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttatattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccgtaccgt tggcgcgggc gttgttgcta aagttctggg ctaattacac
      961 gttaattagt tttgaattga aaagggcgct tcggcgccct ttttgctgcc cgtaatttga
     1021 gctcgaaata tttagtcgta attttgctcg ctgaactatg gcgcaaataa aactgtgcta
     1081 ttgtaatcat aaccattctc atttacactt tgtgcagaat ttgaacggga gcaatcatgt
     1141 acgtttgttt gtgtaatggt gtaagtgaca aaaaaattcg ccaggcagtc cgtcagtttc
     1201 atccgcaatc tttccaacaa ttgcgtaagt ttattcctgt gggaaatcaa tgcggtaagt
     1261 gcattcgcgc cgcgcgcgaa gtgatgcagg acgagttgat gcagatgcca gaatttaaag
     1321 agattgcctg aagcccacgc ttattttttg acatcctgct agccccatct acgctctaat
     1381 agtggaagcg gagggactat aaaatgaaag gtgatgttaa aatcataaat tatctcaata
     1441 aactattggg aaatgagctt gtcgcaatca atcagtattt tctccatgcc cggatgttta
     1501 aaaactgggg cctgatgcgt ctcaatgacg tcgaatacca tgaatccatc gatgagatga
     1561 aacacgctga taaatatatc gagcgtattt tgtttttaga aggaatcccc aacttacagg
     1621 atttaggcaa actggggatt ggtgaagatg tcgaggagat gctgcaatct gacctgcgat
     1681 tagaactgga aggcgcgaag gatctgcgtg aggctatcgc atatgccgac agcgttcatg
     1741 actatgttag ccgcgatatg atgatcgaga tccttgctga tgaagaaggt catatcgact
     1801 ggctcgaaac tgaactggat ctcatcggta aaatcggcct acaaaattac ctgcaatcac
     1861 aaattaaagt taaagattaa cggcctatca gcaaactatc ccagcctata acgaaacccg
     1921 cagccgccag aaatggccca aaaggcagcg ggttttttaa tgattctttt cctctcatga
     1981 gcagaccaat aactacggct ccgcaagcga atgaggcagc aaggaaaacc agtcgtggta
     2041 agaaggccca tgaatgccag gcgccaaggg cggcgagaaa cttcacatca ccatagccta
     2101 atccttcttt atgtcgcagt atgcgatacc cccagtaaat gacagcgaat gtgccgtaac
     2161 caacaattgc cccccataac gcatcggcta aaccatcagg atgacaaact tgatagaaca
     2221 aaagacctga ccagagtaac gggcacgtaa atctgtcagg taacaagcca tgctttgcat
     2281 cccagaaaaa aagtagggct gataaacaag cgtacaaaat tagaaaggga agggtagtgt
     2341 ccataaaaca tcctctgtaa ttggagtatc accatcgaat aactccggat gctcagcaac
     2401 agagagagag cgctgatttg ttaacctgat tccggtgaaa aacgctatct tcacaacctt
     2461 acaaatttcg tggaatgcag ctcgcaatct gcgcgttgcg attaatttct acccgatgct
     2521 tgcgtcaggc tcagatttgc gtataatgcg cgggcttgtc gtagttgaca gcaggttcaa
     2581 tctgaacctc agttagccga atttggctac ctaacaatgc tcccaatcgg ggagctacgt
     2641 aagaacggtt acactctccc atcaatcgta atgggtctga ggagtaatca ttttcgttta
     2701 taaaataatt ggagctctgg tctcatgcag aaccaaagaa tccgtatccg cctgaaagcg
     2761 tttgatcatc gtctgatcga tcaagcaacc gcggaaatcg tcgagactgc caagcgcact
     2821 ggtgcgcagg tccgtggtcc gatcccgctg ccgacacgca aagagcgctt cactgttctg
     2881 atctccccgc acgtcaacaa agacgcgcgc gatcagtacg aaatccgtac tcacttgcgt
     2941 ctggttgaca tcgttgagcc aaccgagaaa accgttgatg ctctgatgcg tctggatctg
     3001 gctgccggtg tagacgtgca gatcagcctg ggttaatcag gtcattgagc gattgagagg
     3061 ttgaaacaat gattggttta gtcggtaaaa aagtgggtat gacccgtatc ttcacagaag
     3121 acggcgtttc tatcccagta accgtaatcg aagttgaagc aaaccgcgtt actcaggtta
     3181 aagacctggc taacgatggc taccgtgcta ttcaggtgac caccggtgct aaaaaagcta
     3241 accgtgtgac caagcctgaa gctggccact tcgctaaagc tggcgtagaa gctggccgtg
     3301 gtctgtggga attccgcctg gctgaaggcg aagagttcac tgtaggtcag agcattagcg
     3361 ttgaactgtt tgctgacgtt aaaaaagttg acgtaactgg cacctctaaa ggtaaaggtt
     3421 tcgcaggtac cgttaagcgc tggaacttcc gtacccagga cgctactcac ggtaactcct
     3481 tgtctcaccg cgttccgggt tctatcggtc agaaccagac tccgggcaaa gtgttcaaag
     3541 gcaagaaaat ggcaggtcag atgggtaacg aacgtgtaac cgttcagagc cttgacgtag
     3601 tacgcgttga cgctgagcgc aacctgctgc tggttaaagg tgctgtcccg ggtgcaaccg
     3661 gtagcgacct gatcgttaaa ccagctgtga aggcgtaagg agatagcaat ggaattagta
     3721 ttgaaagacg cgcagagcgc gctgactgtt tccgaaacta ccttcggtcg tgatttcaac
     3781 gaagcgctgg ttcaccaggt tgttgttgct tatgcagctg gtgctcgtca gggtactcgt
     3841 gctcagaaga ctcgtgctga agtaactggt tccggtaaaa aaccgtggcg ccagaaaggc
     3901 accggccgtg cgcgttctgg ttctatcaag agcccgatct ggcgttctgg tggcgtgacc
     3961 tttgctgctc gtccgcagga ccacagtcaa aaagttaaca agaagatgta ccgcggcgcg
     4021 ctgaaaagca tcctgtccga actggtacgt caggatcgtc tgatcgttgt cgagaagttc
     4081 tctgtagaag cgccgaaaac taagctgctg gcacagaaac tgaaagacat ggctctggaa
     4141 gatgtgctga tcatcaccgg tgagctggac gaaaacctgt tcctggctgc gcgcaacctg
     4201 cagaaggttg acgtacgcga tgcaactggt atcgacccgg ttagcctgat cgccttcgac
     4261 aaagtcgtaa tgactgctga tgctgttaag caagttgagg agatgctggc atgattcgtg
     4321 aagaacgtct gctgaaggtg ctgcgtgcac cgcacgtttc tgaaaaagcg tctactgcga
     4381 tggaaaaatc caacaccatc gtactcaaag ttgctaaaga cgcgaccaaa gcagaaatca
     4441 aagctgctgt gcagaaactg tttgaagtcg aagtcgaagt cgttaacacc ctggtagtta
     4501 aagggaaagt taaacgtcac ggacagcgta tcggtcgtcg tagcgactgg aaaaaagctt
     4561 acgtcaccct gaaagaaggc cagaatctgg acttcgttgg cggcgctgag taagtcggag
     4621 gagtaataca atggcagttg ttaaatgtaa accgacatct ccgggtcgtc gccacgtagt
     4681 taaagtggtt aaccctgagc tgcacaaggg caaacctttt gctccgttgc tggaaaaaaa
     4741 cagcaaatcc ggtggtcgta acaacaatgg ccgtatcacc actcgtcata tcggtggtgg
     4801 ccacaagcag gcttaccgta ttgttgactt caaacgcaac aaagacggta tcccggcagt
     4861 tgttgaacgt cttgagtacg atccgaaccg ttccgcgaac atcgcgctgg ttctgtacaa
     4921 agacggtgaa cgccgttaca tcctggcccc taaaggcctg aaagctggcg accagattca
     4981 gtctggcgtt gatgctgcaa tcaaaccagg taacaccctg ccgatgcgca acatcccggt
     5041 tggttctact gttcataacg tagaaatgaa accaggtaaa ggcggtcagc tggcacgttc
     5101 cgctggtact tacgttcaga tcgttgctcg tgatggtgct tatgtcaccc tgcgtctgcg
     5161 ttctggtgaa atgcgtaaag tagaagcaga ctgccgtgca actctgggcg aagttggcaa
     5221 tgctgagcat atgctgcgcg ttctgggtaa agcaggtgct gcacgctggc gtggtgttcg
     5281 tccgaccgtt cgcggtaccg cgatgaaccc ggtagaccac ccacatggtg gtggtgaagg
     5341 tcgtaacttt ggtaagcacc cggtaactcc gtggggcgtt cagaccaaag gtaagaagac
     5401 ccgcagcaac aagcgtactg ataaattcat cgtacgtcgc cgtagcaaat aattttagag
     5461 gataagccat gccacgttct ctcaagaaag gtccttttat tgacctgcac ttgctgaaga
     5521 aggtagagaa agcggtggaa agcggagaca agaagcccct gcgcacttgg tcccgtcgtt
     5581 caacgatctt tcctaacatg atcggtttga ccatcgctgt ccataatggt cgtcagcacg
     5641 ttccggtatt tgtaaccgac gaaatggtcg gtcacaaact gggtgaattc gcaccgactc
     5701 gtacttatcg cggccacgct gctgataaaa aagcgaagaa gaaataaggt aggaggaaga
     5761 gatggaaact attgctaaac atcgccatgc tcgttcttct gctcagaagg ttcgccttgt
     5821 tgctgacctg attcgcggta agaaagtgtc gcaggctctg gatattttga cctacaccaa
     5881 caagaaaacg gctgtacagg tcaagaaagt tctggaatct gccattgcta acgctgaaca
     5941 caacgatggc gctgacattg acgatctgaa agttacgaaa attttcgtag acgaaggccc
     6001 gagcatgaag cgcattatgc cgcgtgcaaa aggtcgtgca gatcgcatct tgaagcgcac
     6061 cagccacatc actgtggttg tgtccgatcg ctgagactct ggagactagc aatgggtcag
     6121 aaagtacatc ctaatggtat tcgcttgggt attgtaaaac catggaactc tacttggttt
     6181 gcgaacacca aagaattcgc tgacaacctg gacagcgatt ttaaagtacg tcagtacctg
     6241 actaaggaac tggctaaagc gtccgtatct cgtatcgtta tcgagcgtcc ggctaagagc
     6301 atccgtgtaa ccattcacac tgctcgcccg ggtatcgtta tcggtaaaaa aggtgaagac
     6361 gtagaaaaac tgcgtaaggt cgtagcggac atcgctggcg ttcctgcaca gatcaacatc
     6421 gccgaagttc gtaagcctga actggacgca aaactggttg ctgacagcat cacttctcag
     6481 ctggaacgtc gcgttatgtt ccgtcgtgct atgaagcgtg ctgtacagaa cgcaatgcgt
     6541 ctgggcgcta aaggtattaa agttgaagtt agcggccgtc tgggcggcgc ggaaatcgca
     6601 cgtaccgaat ggtaccgcga aggtcgcgta ccgctgcaca ctctgcgtgc tgacatcgac
     6661 tacaacacct ctgaagcgca caccacttac ggtgtaatcg gcgttaaagt gtggatcttc
     6721 aaaggcgaga tcctgggtgg tatggctgct gttgaacaac cggaaaaacc ggctgctcag
     6781 cctaaaaagc agcagcgtaa aggccgtaaa taaggagcgt cgctgatgtt acaaccaaag
     6841 cgtacaaaat tccgtaaaat gcacaaaggc cgtaaccgcg gtctggcgca gggtacggat
     6901 gttagcttcg gcagcttcgg tctgaaagct gttggccgtg gtcgtctgac tgcccgtcag
     6961 atcgaagcag cacgtcgtgc tatgacccgt gcagttaagc gtcaaggtaa gatctggatc
     7021 cgtgtgttcc cggacaaacc gatcactgaa aagccgctgg cagtgcgtat gggtaaaggt
     7081 aaaggtaacg tggagtattg ggttgccttg attcagccgg gtaaagtcct gtatgaaatg
     7141 gacggtgttc cggaagagct ggcccgtgaa gcattcaagc tggcagcagc gaaactgccg
     7201 attaaaacca cctttgtaac taagacggtg atgtaatgaa agcaaaagag ctgcgtgaga
     7261 agagcattga agagctgaac accgagctgc tgaacctgct gcgtgagcag ttcaacctgc
     7321 gtatgcaggc tgcaagtggc cagctgcaac agtctcacct gttgaagcaa gtgcgtcgcg
     7381 atgtcgcacg cgttaagact ttactgaacg agaaggcggg tgcgtaatga ccgataaaat
     7441 ccgtactctg caaggtcgcg ttgttagcga caaaatggag aaatccattg ttgttgctat
     7501 cgaacgtttt gtgaaacacc cgatctacgg taaattcatc aagcgtacga ccaaactgca
     7561 cgtacatgac gagaacaacg aatgcggtat cggtgacgtg gttgaaatcc gcgaatgccg
     7621 tccgctgtcc aagactaaat cctggacgct ggttcgcgtt gtagagaaag cggttctgta
     7681 atacagtaca ctctctcgat acgaataaac ggctcagaaa tgagccgttt attttttcta
     7741 cccatatcct tgaagcggtg ttataatgcc gcgccctcga tatggggatt tttaacgacc
     7801 tgattttcgg gtctcagtag tagttgacat tagcggagca ctaaaatgat ccaagaacag
     7861 actatgctga acgtcgccga caactccggt gcacgtcgcg taatgtgtat caaggttctg
     7921 ggtggctcgc accgtcgcta cgcaggcgta ggcgacatca tcaagatcac catcaaagaa
     7981 gcaattccgc gtggtaaggt caaaaaaggt gatgtgctga aggcggtagt ggtgcgcacc
     8041 aagaagggtg ttcgtcgccc ggacggttct gtcattcgct tcgatggtaa tgcttgtgtt
     8101 cttctgaaca acaacagcga gcagcctatc ggtacgcgta tttttgggcc ggtaactcgt
     8161 gagcttcgta gtgagaagtt catgaaaatt atctctctgg caccagaagt actctaagga
     8221 gcgaatcatg gcagcgaaaa tccgtcgtga tgacgaagtt atcgtgttaa ccggtaaaga
     8281 taaaggtaaa cgcggtaaag ttaagaatgt cctgtcttcc ggcaaggtca ttgttgaagg
     8341 tatcaacctg gttaagaaac atcagaagcc ggttccggcc ctgaaccaac cgggtggcat
     8401 cgttgaaaaa gaagccgcta ttcaggtttc caacgtagca atcttcaatg cggcaaccgg
     8461 caaggctgac cgtgtaggct ttagattcga agacggcaaa aaagtccgtt tcttcaagtc
     8521 taacagcgaa actatcaagt aatttggagt agtacgatgg cgaaactgca tgattactac
     8581 aaagacgaag tagttaaaaa actcatgact gagtttaact acaattctgt catgcaagtc
     8641 cctcgggtcg agaagatcac cctgaacatg ggtgttggtg aagcgatcgc tgacaaaaaa
     8701 ctgctggata acgcagcagc agacctagca gcaatctccg gtcaaaaacc gctgatcacc
     8761 aaagcacgca aatctgttgc aggcttcaaa atccgtcagg gctatccgat cggctgtaaa
     8821 gtaactctgc gtggcgaacg catgtgggag ttctttgagc gcctgatcac tattgctgta
     8881 cctcgtatcc gtgacttccg tggcctgtcc gctaagtctt tcgacggtcg tggtaactac
     8941 agcatgggtg tccgtgagca gatcatcttc ccagaaatcg actacgataa agtcgaccgc
     9001 gttcgtggtt tggatattac cattaccact actgcgaaat ctgacgaaga aggccgcgct
     9061 ctgctggctg cctttgactt cccgttccgc aagtaaggta gggttactaa atggctaagc
     9121 aatcaatgaa agcacgcgaa gtaaaacgcg tagctttagc tgataaatac ttcgcgaaac
     9181 gcgctgaact gaaagcgatc atctctgatg tgaacgcttc cgacgaagat cgttggaacg
     9241 ctgttctcaa gctgcagact ctgccgcgtg attccagccc gtctcgtcag cgtaaccgct
     9301 gccgtcaaac aggtcgtccg catggtttcc tgcggaagtt cgggttgagc cgtattaagg
     9361 tccgtgaagc cgctatgcgc ggtgaaatcc cgggtctgaa aaaggctagc tggtaattgt
     9421 caccaattga atcacgggag taaagacaga tgagcatgca agatccgatc gcggatatgc
     9481 tgacccgtat ccgtaacggt caggccgcga acaaagctgc ggtcaccatg ccttcctcca
     9541 agctgaaagt ggcaatcgcc aacgtgctga aggaagaagg ttttattgaa gattttaaag
     9601 ttgaaggcga caccaagcct gaactggaac ttactctgaa gtatttccag ggcaaagctg
     9661 ttgtagaaag cattcagcgt gtcagccgcc caggtctgcg catctataaa cgtaaagatg
     9721 agctgccgaa agttatggcg ggtctgggta tcgcagttgt ttctacctct aaaggtgtta
     9781 tgactgatcg tgcagcgcgc caggctggtc ttggtggcga aattatctgc tacgtagcct
     9841 aatcggagga aaaaatgtct cgtgttgcta aagcaccggt cgttgttcct gccggcgttg
     9901 acgtaaaaat caacggtcag gttattacga tcaaaggtaa aaacggcgag ctgactcgta
     9961 ctctcaacga tgctgttgaa gttaaacatg cagataatac cctgaccttc ggtccgcgtg
    10021 atggttacgc agacggttgg gcacaggctg gtaccgcgcg tgccctgctg aactcaatgg
    10081 ttatcggtgt taccgaaggc ttcactaaga agctgcagct ggttggtgta ggttaccgtg
    10141 cagcggttaa aggcaatgtg attaacctgt ctctgggttt ctctcatcct gttgaccatc
    10201 agctgcctgc gggtatcact gctgaatgtc cgactcagac tgaaatcgtg ctgaaaggcg
    10261 ctgataagca ggtgatcggc caggttgcag cggatctgcg cgcctaccgt cgtcctgagc
    10321 cttacaaagg caagggtgtt cgttacgccg acgaagtcgt gcgtaccaaa gaggctaaga
    10381 agaagtaagg taacactatg gataagaaat ctgctcgtat ccgtcgtgcg acccgcgcac
    10441 gccgcaagct ccaggagctg ggcgcaactc gcctggtggt acatcgtacc ccgcgtcaca
    10501 tttacgcaca ggtaattgca ccgaacggtt ctgaagttct ggtagctgct tctactgtag
    10561 aaaaagctat cgctgaacaa ctgaagtaca ccggtaacaa agacgcggct gcagctgtgg
    10621 gtaaagctgt cgctgaacgc gctctggaaa aaggcatcaa agatgtatcc tttgaccgtt
    10681 ccgggttcca atatcatggt cgtgtccagg cactggcaga tgctgcccgt gaagctggcc
    10741 ttcagttcta aggtagaggt gtaagatggc tcacatcgaa aaacaagctg gcgaactgca
    10801 ggaaaagctg atcgcggtaa accgcgtatc taaaaccgtt aaaggtggtc gtattttctc
    10861 cttcacagct ctgactgtag ttggcgatgg taacggtcgc gttggttttg gttacggtaa
    10921 agcgcgtgaa gttccagcag cgatccagaa agcgatggaa aaagcccgtc gcaatatgat
    10981 taacgtcgcg ctgaataacg gcactctaca acaccctgtt aaaggtgttc acacgggttc
    11041 tcgcgtattc atgcagccgg cttccgaagg taccggtatc atcgccggtg gtgcaatgcg
    11101 cgccgttctg gaagtcgctg gggttcataa cgttctggct aaagcatatg gttccaccaa
    11161 cccgatcaac gtggttcgtg caactattga tggcctggaa aatatgaatt ctccagaaat
    11221 ggtcgctgcc aagcgtggta aatccgttga agaaattctg gggaaataaa ccatggcaaa
    11281 gactattaaa attactcaaa cccgcagtgc aatcggtcgt ctgccgaaac acaaggcaac
    11341 gctgcttggc ttgggtctgc gtcgtattgg tcacaccgta gagcgcgagg atactcctgc
    11401 tattcgcggt atgatcaacg cggtttcctt catggttaaa gttgaggagt aagagatgcg
    11461 tttaaatact ctgtctccgg ccgaaggctc caaaaaggcg ggtaaacgcc tgggtcgtgg
    11521 tatcggttct ggcctcggta aaaccggtgg tcgtggtcac aaaggtcaga agtctcgttc
    11581 tggcggtggc gtacgtcgcg gtttcgaggg tggtcagatg cctctgtacc gtcgtctgcc
    11641 gaaattcggc ttcacttctc gtaaagcagc gattacagcc gaagttcgtc tatctgacct
    11701 ggctaaagta gaaggcggtg ttgtagacct gaacacgctg aaagcggcta acattatcgg
    11761 tatccagatc gagttcgcga aagtgatcct ggctggcgaa gtaacgactc cggtaactgt
    11821 tcgtggcctg cgtgttacta aaggcgctcg tgctgctatc gaagctgctg gcggtaaaat
    11881 cgaggaataa gtagcagatg gctaaacaac cgggattaga ttttcaaagt gccaaaggtg
    11941 gcttaggcga gctgaaacgc agactgctgt ttgttatcgg tgcgctgatt gtgttccgta
    12001 ttggctcttt tattccgatc cctggtattg atgccgctgt acttgccaaa ctgcttgagc
    12061 aacagcgagg caccatcatt gagatgttta acatgttctc tggtggtgct ctcagccgtg
    12121 cttctatctt tgctctgggg atcatgccgt atatttcggc gtcgatcatt atccagctgc
    12181 tgacggtggt tcacccaacg ttggcagaaa ttaagaaaga aggggagtct ggtcgtcgta
    12241 agatcagcca gtacacccgt tacggtactc tggtgctggc aatattccag tcgatcggta
    12301 ttgctaccgg tctgccgaat atgcctggta tgcaaggcct ggtgattaac ccgggctttg
    12361 cattctactt caccgctgtt gtaagtctgg tcacaggaac gatgttcctg atgtggttgg
    12421 gcgaacagat cactgaacga ggtatcggca acggtatttc aatcattatc ttcgccggta
    12481 ttgtcgcggg actcccgcca gccattgccc atactatcga gcaagcgcgt caaggcgacc
    12541 tgcacttcct cgtgttgctg ttggttgcag tattagtatt tgcagtgacg ttctttgttg
    12601 tgtttgttga gcgtggtcaa cgccgcattg tggtaaacta cgcgaaacgt cagcaaggtc
    12661 gtcgtgtcta tgctgcacag agcacacatt taccgctgaa agtgaatatg gcgggggtaa
    12721 tcccggcaat cttcgcttcc agtattattc tgttcccggc gaccatcgcg tcatggttcg
    12781 ggggcggtac tggttggaac tggctgacaa caatttcgct gtatttgcag cctgggcaac
    12841 cgctttatgt gttactctat gcgtctgcaa tcatcttctt ctgtttcttc tacacggcgt
    12901 tggttttcaa cccgcgtgaa acagcagata acctgaagaa gtccggtgca tttgtaccag
    12961 gaattcgtcc gggagagcaa acggcgaagt atatcgataa agtaatgacc cgcctgacct
    13021 tggttggtgc gctgtatatt acctttatct gcctgatccc ggagttcatg cgtgatgcaa
    13081 tgaaagtacc gttctacttc ggtgggacct cactgcttat cgttgttgtc gtgattatgg
    13141 actttatggc tcaagtgcaa actctgatga tgtccagtca gtatgagtct gcattgaaga
    13201 aggcgaacct gaaaggctac ggccgataat tggtcgcccg agaagttacg gagagtaaaa
    13261 atgaaagttc gtgcttccgt caagaaatta tgccgtaact gcaaaatcgt taagcgtgat
    13321 ggtgtcatcc gtgtgatttg cagtgccgag ccgaagcata aacagcgcca aggctgattt
    13381 tttcgcatat ttttcttgca aagttgggtt gagctggcta gattagccag ccaatctttt
    13441 gtatgtctgt acgtttccat ttgagtatcc tgaaaacggg cttttcagca tggaacgtac
    13501 atattaaata gtaggagtgc atagtggccc gtatagcagg cattaacatt cctgatcata
    13561 agcatgccgt aatcgcatta acttcgattt atggcgtcgg caagacccgt tctaaagcca
    13621 tcctggctgc agcgggtatc gctgaagatg ttaagatcag tgagctgtct gaaggacaaa
    13681 tcgacacgct gcgtgacgaa gttgccaaat ttgtcgttga aggtgatctg cgccgtgaaa
    13741 tcagcatgag catcaagcgc ctgatggatc ttggttgcta tcgcggtttg cgtcatcgtc
    13801 gtggtctccc ggttcgcggt cagcgtacca agaccaacgc acgtacccgt aagggtccgc
    13861 gcaaaccgat caagaaataa tcggggtgat tgaataatgg caaaggcacc aattcgtgca
    13921 cgtaaacgtg taagaaaaca agtctctgac ggcgtggctc atatccatgc ttctttcaac
    13981 aacaccatcg tgactatcac tgatcgtcag ggtaacgcgt tgggttgggc aacagccggt
    14041 ggttccggtt tccgtggttc tcgcaaatcc actccgtttg cagctcaggt tgcagcagag
    14101 cgttgcgctg acgccgtgaa agaatacggc atcaagaatc tggaagttat ggttaaaggt
    14161 ccgggtccag gccgcgaatc tactattcgt gctctgaacg ccgcaggttt ccgcatcact
    14221 aacattactg atgtgactcc gatccctcat aacggttgtc gtccgccgaa aaaacgtcgc
    14281 gtataacgcc tcgttttcca ggtttgttgg agaaagaaaa tggcaagata tttgggtcct
    14341 aagctcaagc tgagccgtcg tgagggcacc gacttattcc ttaagtctgg cgttcgcgcg
    14401 atcgatacca agtgtaaaat tgaacaagct cctggccagc acggtgcgcg taaaccgcgt
    14461 ctgtctgact atggtgtgca gttgcgtgaa aagcaaaaag ttcgccgtat ctatggtgtg
    14521 ctggagcgtc agttccgtaa ctactacaaa gaagcagcac gtctgaaagg caacaccggt
    14581 gaaaacctgt tggctctgct ggaaggtcgt ctggacaacg ttgtataccg tatgggcttc
    14641 ggtgccactc gtgcagaagc acgtcagctg gttagccata aagcaattat ggtaaacggt
    14701 cgtgttgtta acatcgcttc ttatcaggtt agtccgaatg acgttgtaag cattcgtgag
    14761 aaagcgaaga agcagtctcg cgtgaaagcc gctctggagc tggctgagca gcgtgaaaag
    14821 ccaacttggc tggaagttga tgctggcaag atggaaggta cgtttaagcg taagccggag
    14881 cgttctgatc tgtctgcgga cattaacgaa cacctgatcg tcgagcttta ctccaagtaa
    14941 agcttagtac caaagagagg acacaatgca gggttctgtg acagagtttc taaaaccgcg
    15001 cctggttgat atcgagcaag tgagttcgac gcacgctaag gtgacccttg agcctttaga
    15061 gcgtggcttt ggccatactc tgggtaacgc actgcgccgt attctgctct catcgatgcc
    15121 gggttgcgcg gtgaccgagg ttgagattga tggtgtacta catgagtaca gcaccaaaga
    15181 aggcgttcag gaagatatcc tggaaatcct gctcaacctg aaagggctgg cggtgagagt
    15241 tcagggcaaa gatgaagtta ttcttacctt gaataaatct ggcattggcc ctgtgactgc
    15301 agccgatatc acccacgacg gtgatgtcga aatcgtcaag ccgcagcacg tgatctgcca
    15361 cctgaccgat gagaacgcgt ctattagcat gcgtatcaaa gttcagcgcg gtcgtggtta
    15421 tgtgccggct tctacccgaa ttcattcgga agaagatgag cgcccaatcg gccgtctgct
    15481 ggtcgacgca tgctacagcc ctgtggagcg tattgcctac aatgttgaag cagcgcgtgt
    15541 agaacagcgt accgacctgg acaagctggt catcgaaatg gaaaccaacg gcacaatcga
    15601 tcctgaagag gcgattcgtc gtgcggcaac cattctggct gaacaactgg aagctttcgt
    15661 tgacttacgt gatgtacgtc agcctgaagt gaaagaagag aaaccagagt tcgatccgat
    15721 cctgctgcgc cctgttgacg atctggaatt gactgtccgc tctgctaact gccttaaagc
    15781 agaagctatc cactatatcg gtgatctggt acagcgtacc gaggttgagc tccttaaaac
    15841 gcctaacctt ggtaaaaaat ctcttactga gattaaagac gtgctggctt cccgtggact
    15901 gtctctgggc atgcgcttgg aaaactggcc accggcaagc atcgctgacg agtaaccgga
    15961 tcacaggtta aggttttact gagaaggata aggtcatgcg ccatcgtaag agtggtcgtc
    16021 aactgaaccg caacagcagc catcgccagg ctatgttccg caatatggca ggttcactgg
    16081 ttcgtcatga aatcatcaag acgactctgc ctaaagcgaa agagctgcgc cgcgtagttg
    16141 agccgctgat tactcttgcc aagactgata gcgttgctaa tcgtcgtctg gcattcgccc
    16201 gtactcgtga taacgagatc gtggcaaaac tgtttaacga actgggcccg cgtttcgcga
    16261 gccgtgccgg tggttacact cgtattctga agtgtggctt ccgtgcaggc gacaacgcgc
    16321 cgatggctta catcgagctg gttgatcgtt cagagaaagc agaagctgct gcagagtaat
    16381 ctgaagcaat gtaaaaaaac ccgccccggc gggttttttt atacccgtag tatccccact
    16441 tatctacaat agctgtactc ttttt
//
LOCUS       DAOFMX010000086        16389 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.086, whole genome shotgun sequence.
ACCESSION   DAOFMX010000086 DAOFMX010000000
VERSION     DAOFMX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.086"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="QBA20_003284"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="QBA20_003284"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDQ6293711.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="QBA20_003285"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="QBA20_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDQ6293712.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="QBA20_003286"
     CDS             2339..2554
                     /locus_tag="QBA20_003286"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293713.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="QBA20_003287"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="QBA20_003287"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDQ6293714.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="QBA20_003288"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="QBA20_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDQ6293715.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="QBA20_003289"
     CDS             5920..6417
                     /locus_tag="QBA20_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDQ6293716.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="QBA20_003290"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="QBA20_003290"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDQ6293717.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="QBA20_003291"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="QBA20_003291"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDQ6293718.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="QBA20_003292"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="QBA20_003292"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDQ6293719.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="QBA20_003293"
     CDS             9102..9665
                     /locus_tag="QBA20_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDQ6293720.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="QBA20_003294"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="QBA20_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDQ6293721.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="QBA20_003295"
     CDS             complement(10257..11237)
                     /locus_tag="QBA20_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDQ6293722.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="QBA20_003296"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="QBA20_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDQ6293723.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRLAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="QBA20_003297"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="QBA20_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDQ6293724.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="QBA20_003298"
     CDS             12540..12830
                     /locus_tag="QBA20_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDQ6293725.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="QBA20_003299"
     CDS             12838..13431
                     /locus_tag="QBA20_003299"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDQ6293726.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="QBA20_003300"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="QBA20_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDQ6293727.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14874..15837
                     /locus_tag="QBA20_003301"
                     /pseudo
     CDS             14874..15837
                     /locus_tag="QBA20_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15882..16385
                     /locus_tag="QBA20_003302"
     CDS             15882..16385
                     /locus_tag="QBA20_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDQ6293728.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4059 a         4217 c         4127 g         3986 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatt cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc agggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccacctgc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcctggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgcc
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgggct
    12061 cattgtgatt tttacaaccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtac gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgtccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaatcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt tttatgaaaa
    14881 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
    14941 taaacgccgc agaaaaagtg gcgttaaagt tagcacacaa cctggaaaga agtcatgttg
    15001 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
    15061 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gaattgttgc
    15121 aaaatggcgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
    15181 tttatgctat ctataatttg ccgttcttat taagttgttt ttggtgaagt tggtaaagaa
    15241 attatggact caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtact
    15301 cgcagtttct atgcgaaaaa accgattaca aagccagaag atcttaaagg actgaaaatt
    15361 cgtgttcagc caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact
    15421 ccaatttcat ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa
    15481 aataacgtgc cttcctggat gcagactcga catattgaaa ttgccaaagt tttttctgaa
    15541 gatgaacacg cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta
    15601 accccagaac agcaacaaat cctcgaaaca gcggcgaaga aatccgaagc ttatcaacaa
    15661 aaattgtggg aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa
    15721 attgtaaaag tggataaagc gccattccgt aaagccgtgc aacctctgtt tgacgacttc
    15781 aaaaaagatc caaaacaagc tgctttgtta gagaaattcg acaacgctgc tcaataatat
    15841 tccgggccag cctctggccc tgattttcca ggatgcttac aatgatattt aaccgcctga
    15901 aacttgcggt agaccgcgtc attgccgcgt tttctgtcgc cgtaatgctt gctctggttg
    15961 tatgcgtagt ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg
    16021 atgagctggc acgctttttg atgatctggg taggattatt aggtgctgca tataccgtcg
    16081 gcgcgcagcg acatctctct attgatttat ttgctctcgc cctgaataag cgcaaacaat
    16141 tacttcttag tatagtcatt aacgttctca ttttaggttt tgccggttcg gtcattgtga
    16201 ctggtggcct gaaattaatt gataaaacac tggcaacatc acaggtatct gccgctatgc
    16261 aaataccgat gggatacgta tatatcattc tgccattaag tggagtggta atgatgtttt
    16321 atgccttgtg ttttattaat caaagcattc aacaactgaa acaacctgcg caggaggcca
    16381 gttaatgga
//
LOCUS       DAOFMX010000087        15932 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.087, whole genome shotgun sequence.
ACCESSION   DAOFMX010000087 DAOFMX010000000
VERSION     DAOFMX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.087"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            447..1505
                     /gene="citC"
                     /locus_tag="QBA20_003303"
     CDS             447..1505
                     /gene="citC"
                     /locus_tag="QBA20_003303"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDQ6293729.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1520..1816
                     /gene="citD"
                     /locus_tag="QBA20_003304"
     CDS             1520..1816
                     /gene="citD"
                     /locus_tag="QBA20_003304"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDQ6293730.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1813..2721
                     /gene="citE"
                     /locus_tag="QBA20_003305"
     CDS             1813..2721
                     /gene="citE"
                     /locus_tag="QBA20_003305"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDQ6293731.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2732..4264
                     /gene="citF"
                     /locus_tag="QBA20_003306"
     CDS             2732..4264
                     /gene="citF"
                     /locus_tag="QBA20_003306"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDQ6293732.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4268..4819
                     /gene="citX"
                     /locus_tag="QBA20_003307"
     CDS             4268..4819
                     /gene="citX"
                     /locus_tag="QBA20_003307"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDQ6293733.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4794..5672
                     /gene="citG"
                     /locus_tag="QBA20_003308"
     CDS             4794..5672
                     /gene="citG"
                     /locus_tag="QBA20_003308"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDQ6293734.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5723..7185
                     /gene="citT"
                     /locus_tag="QBA20_003309"
                     /pseudo
     CDS             5723..7185
                     /gene="citT"
                     /locus_tag="QBA20_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7299..8105
                     /gene="rna"
                     /locus_tag="QBA20_003310"
     CDS             7299..8105
                     /gene="rna"
                     /locus_tag="QBA20_003310"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDQ6293735.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8334..8744
                     /gene="rnk"
                     /locus_tag="QBA20_003311"
     CDS             8334..8744
                     /gene="rnk"
                     /locus_tag="QBA20_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDQ6293736.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8975..10213)
                     /locus_tag="QBA20_003312"
     CDS             complement(8975..10213)
                     /locus_tag="QBA20_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HDQ6293737.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGVTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10434..10862
                     /gene="uspG"
                     /locus_tag="QBA20_003313"
     CDS             10434..10862
                     /gene="uspG"
                     /locus_tag="QBA20_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDQ6293738.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10983..12548)
                     /gene="ahpF"
                     /locus_tag="QBA20_003314"
     CDS             complement(10983..12548)
                     /gene="ahpF"
                     /locus_tag="QBA20_003314"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDQ6293739.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12793..13356)
                     /gene="ahpC"
                     /locus_tag="QBA20_003315"
     CDS             complement(12793..13356)
                     /gene="ahpC"
                     /locus_tag="QBA20_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDQ6293740.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13728..14474
                     /gene="dsbG"
                     /locus_tag="QBA20_003316"
     CDS             13728..14474
                     /gene="dsbG"
                     /locus_tag="QBA20_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDQ6293741.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14684..15586
                     /locus_tag="QBA20_003317"
     CDS             14684..15586
                     /locus_tag="QBA20_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6293742.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEYPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15733..15932
                     /locus_tag="QBA20_003318"
                     /pseudo
     CDS             15733..15932
                     /locus_tag="QBA20_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3953 a         4118 c         3855 g         4006 t
ORIGIN      
        1 agatacgcag cggaaatgcc agtctttgga aaaatgcaaa ctgtttattc tcgttaagct
       61 gcaacattgt tttccttgcg ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt
      121 gaatcaaaat acatagatat taattatgat cgtggcaaat tttattcatt aaatcaatta
      181 aatcacttaa acctgcgagt catttcgtaa ttacattaat taaaaaactt aaaaccatta
      241 aataaataaa aaaccactaa ctctatgtga aataaatcaa aatttcacgc cgaaatactc
      301 cttaggatgt atagcgaaag gagaaaaaga tatacctcga tcacccccct ttctcccaag
      361 tgaaaataaa aggttatcag tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa
      421 catgcacctt caggatacta tttattatgt tcggcaatga tattttcacc cgcgtaaaac
      481 gttcagaaaa taaaaaaatg gcggaaatcg cccaattcct gcatgaaaat gatttgagcg
      541 ttgacaccac agtcgaagta tttattaccg taacccgcga tgaaaagctt atcgcgtgcg
      601 gtggaattgc cggaaatatt attaaatgcg ttgctatcag tgaatccgtt cgcggtgaag
      661 gactggcgct gacattagcc actgaactga taaacctcgc ctatgagcgg cacagcacgc
      721 atctgtttat ttataccaaa accgaatacg aggcgctgtt ccgccagtgc ggtttttcca
      781 cgctgacctg cgtacccggc gtgatggtgc tgatggaaaa cagcgccacg cgactgaaac
      841 gctatgccga atcgctgaaa aaatttcgtc atccagggaa caagattggc tgcattgtga
      901 tgaacgccaa tccctttacg aatggtcacc gttatctgat tcaacaagct gcggcacagt
      961 gcgactggtt gcatctgttt ttagtcaaag aagattcttc acgcttcccc tatgaagacc
     1021 ggctggatct ggtgttaaaa ggcaccgccg atattccacg cctgactgtg catcgcggct
     1081 cagaatacat catctcccgc gctacgttcc cttgctactt cattaaagaa cagagcgtca
     1141 ttaaccattg ttacaccgaa attgatctga agattttccg tcagtacctc gctcccgcgc
     1201 ttggtgtaac tcaccgcttt gtcggtactg aacccttttg tcgcgttacc gcccagtaca
     1261 accaggatat gcgctactgg ctggaaacgc cgactatctc cgcagcgccc atcgaactgg
     1321 ttgaaattga gcggctgcgt taccaggaga tgccgatatc cgcttcccgg gtacgtcaac
     1381 tgctggcgaa aaacgatctc acggctatcg cgccgctggt ccctgcagtc acgctgcatt
     1441 atttgcagaa cctgcttgag cactcccgcc aggacgcggc agctcgtcaa aagacccccg
     1501 catgagaaac aggtgaaaaa tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc
     1561 tggggatgtg atgatacgca tcgccccact cgatacgcag gatatcgacc tgcaaatcaa
     1621 tagcagcgtt gagaaacagt ttggcgatgc aattcgcacc accattctgg acgttctcgc
     1681 ccgctacaac gtgcgcggcg tacagctgaa tgtcgatgac aaaggcgcac tggactgcat
     1741 tttacatgca cgactggaag ccctgctggc acgcgccagc ggtatcccgg ctctgccatg
     1801 ggaggattgc caatgatttc cgcttcgctg caacaacgta aaactcgcac ccgccgcagc
     1861 atgttgtttg tgcctggtgc caatgccgcg atggtcagca actccttcat ctacccggct
     1921 gatgccctga tgtttgacct cgaagactcc gtggcattac gtgaaaaaga caccgcccgc
     1981 cgcatggttt atcacgcgct gcaacatccg ctgtatcgcg atattgaaac cattgtgcgt
     2041 gtcaacgcgc tggattccga atggggtgtt aacgacctgg aagccgtcgt tcgcggtggt
     2101 gcggacgttg tgcgtctgcc gaaaaccgat accgctcagg atgttctgga tattgaaaaa
     2161 gagatcctgc gtatagaaaa agcctgtggt cgtgaacccg gcagcaccgg cctgctggcg
     2221 gcgattgaat ctccgctggg cattacccgc gcagtggaaa tcgctcacgc ttccgagcgt
     2281 ttgatcggta tcgccctcgg tgcagaagac tatgtgcgca acctgcgtac agaacgctcc
     2341 ccggaaggaa ctgaactgct gttcgcacgc tgttccattt tgcaggccgc gcgctctgcg
     2401 ggtattcagg cgttcgatac cgtctattcc gacgctaaca acgaagccgg attcctgcaa
     2461 gaagccgccc acatcaaaca gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag
     2521 attgatctgc tgcacaacct ctacgcaccg acccagaaag aagtggatca cgcccaccgc
     2581 gtcgtagaag ccgctgaagc cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc
     2641 aagatggtgg acggtccggt tatcgatcgc gcccgtctgg tgctctcccg tgcagaactt
     2701 tccggcatcc gcgaagaata aggcaatcaa aatgacgcag aaaattgaac aatctcaacg
     2761 acaagaacgg gtagcggcct ggaatcgtcg cactgaatgc gatcttgccg ctttccagaa
     2821 ctcaccaaag caaacctacc aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct
     2881 ggaagaagcg attcgtcgct caggtttaca ggacggcatg acggtttcct tccatcacgc
     2941 tttccgtggc ggtgacctga ccgtcaatat ggtgatgaac gtcatcgcga agatgggctt
     3001 taaaaacctg accctggcgt ccagctccct gagtgattgc catgcgccgc tggtagaaca
     3061 cattcgccag ggcgtggtta cccgcattta tacctctggc ctacgcggtc cactggcgga
     3121 agagatctcc cgtggtctgc tggcggaacc ggtgcagatc cactctcacg gcggtcgtgt
     3181 gcatctggta cagagcggcg aactgaatat cgacgtggct ttcctcggcg tcccgtcctg
     3241 tgatgaattc ggtaatgcca acggctacac cggtaaagcc tgctgcggct ccctcggcta
     3301 tgcaatggtt gatgccgaca acgcaaaaca ggtcgtgatg cttaccgaag aactgctgcc
     3361 ttatccgcat aatccggcaa gcattgagca agatcaggtt gatttgatcg tcaaagttaa
     3421 tcgcgttggc gatgctgcaa aaatcggcgc tggtactacc cgtatgacca ctaacccgcg
     3481 cgaactgctt attgcccgta gcgctgcgga tgtgattgtc aactctggct acttcaaaga
     3541 aggtttctcc atgcaaaccg gcaccggcgg cgcttcgctg gcggtaaccc gtttcctgga
     3601 agacaaaatg cgtagccgcg atattcgcgc cgacttcgcc ctcggcggta ttaccgcgac
     3661 gatggttgat ctgcacgaaa aaggtctgat ccgcaaactg ctggatgtgc agagctttga
     3721 cagccatgct gcgcaatcgc tggcccgtaa ccccaatcac atcgaaatca gcgccaacca
     3781 gtacgctaac tggggttcga aaggcgcatc ggttgatcgt ctcgacgtgg tggtactgag
     3841 cgcgctggaa attgacaggc agttcaacgt taacgtgctg accggttctg acggcgtact
     3901 gcgtggcgct tccggtggtc actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt
     3961 cgcgccactg gtacgcggtc gtattccgac tctggtggat aacgtgctga cctgcatcac
     4021 cccgggctcc agtgtcgata ttctggtcac agaccacggt atcgcagtta acccggcacg
     4081 tccggaacta gcagaacgtc tgcaggaagc gggcattaaa gtggtttcca ttgagtggct
     4141 gcgcgaacgt gcgcgtctgc tgaccggtga accacagccg attgaattca ccgaccgcgt
     4201 cgttgccgtt gtgcgttacc gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga
     4261 ataagccatg cacctgcttc ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct
     4321 gctcgtcagc cgggatgaaa ggcaagcacg gcaacacgcc tggctcaagc accatcctgt
     4381 tccactggtc tcctttaccg tggttgcgcc tgggccgatt aaagacagcg aggtcacacg
     4441 ccgaattttt aatcatcgcg tgacagcctt gcgtgcctta gccacaaagc agggctggca
     4501 aattcaggag caggctgcac tggtttccgc cagcgggccg gagggcatgt tgagcattgc
     4561 cgccccggct cgcgacctca agctcgccac cattgagctt gaacatagtc atcctctcgg
     4621 gcggttatgg gatatcgatg tcctgacgcc cgaaggcgaa attctctccc gccgcgacta
     4681 ttcactgccg cctcgccgct gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg
     4741 aaaaacccat caactgaccg atttactcaa ccgcatggag gcactgctga acgatgtcga
     4801 tgcctgcaac gtcaactaaa accacaaagc ttgcgacgtc attaatcgat gagtacgccc
     4861 tgctgggctg gcgcgccatg ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg
     4921 atcgcattaa ctgcggtgcg cacaaagata tggcgctgga agatttccac cgcagcgcgc
     4981 tggcgattca gggctggtta ccccgtttca ttgaatttgg tgcctgtagt gcagaaatgg
     5041 caccggaagc ggtactccac ggattacgcc caattggtat ggcttgcgaa ggtgatatgt
     5101 tccgcgccac tgcgggcgta aacacgcata aaggcagcat tttttcttta gggctgctat
     5161 gtgcggcaat tggccgtttg cttcagctca accagccggt tacgccaaca accgtttgtt
     5221 ctacggcggc aagtttctgc cgtggcctga ccgatcgcga actgcgtacc aataattcac
     5281 gactgacggc aggtcaacgg ttgtaccagc agcttggcct taccggcgca cgcggtgaag
     5341 ccgaagcagg ttatccactg gtgatcaacc acgccttgcc gcattacctc actctgctgg
     5401 atcaggggtt agatcctgaa ctggcattgc tcgatacctt gctcctactg atggctacca
     5461 acggcgatac caacgttgca tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg
     5521 aggcgcaaac attattgcaa aaagggggca ttcgaacccc caccgatctc gattatctcc
     5581 ggcagttcga cagggagtgt atcgaacgaa atctcagtcc aggcggcagt gccgacctgc
     5641 tgatccttac ctggttttta gcacagattt aattatttaa gcacttgata aatttggaaa
     5701 tattaatttt cggagaaccc gtatgtcttt agcaaaagat aatatatgga aactattggc
     5761 cccactggtg gtgatgggtg tcatgtttct tatccctgtc cccgacggta tgccgccgca
     5821 ggcatggcat tacttcgctg tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat
     5881 tccggcaaca gcgatcagtt ttattgcggt tactatttgc gttattggca gtaattacct
     5941 gctctttgat gccaaagaat tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa
     6001 atggggcctg gctggttttt ccagcaccac ggtatggctg gtatttggcg catttatttt
     6061 tgcattaggg tatgaagttt ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt
     6121 catggacaaa cgcacgctga cgttgggtta tgcgattgtc attatcgaca ttctgctggc
     6181 accgtttaca ccgtccaaca ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa
     6241 cctgccgccg ttgtttaaat cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta
     6301 tttgatgtgg atgatggtca ttagtaccag tctgagttcg tccatgtttg tcaccggtgc
     6361 ggaccaaacg tgctgggtct ggagttcgtc agcaaaattg ccggtatcca gattagctgg
     6421 ttgcagtggt tcctctgctt cctgccggtt ggggttatct tgcttatcat tgcgccgtgg
     6481 ctttcctacg tgctgtacaa accggaaatc acacacagtg aagaagtggc aacctgggcg
     6541 ggtgatgaac taaaaaccat gggtgcgctg acacgcagag agtggacgct gattggcctt
     6601 gtattgctca gcttaggttt gtgggtattt ggcagtgaag tcattaatgc tactgcggtt
     6661 ggtctgctgg cagtttcgct aatgctggct ctgcacgttg tgccgtggaa agacattacc
     6721 cgctataaca gcgcatggaa cacgctggtc aacctggcaa ctctggttgt gatggctaac
     6781 ggcctgactc gttctggttt tattgactgg ttcgccggta ccatgagtac gcacctggaa
     6841 ggattctcac caaacgcaac ggtgattgta ctggttctgg tgttctactt tgcacactac
     6901 ctgtttgcca gcctgtctgc gcacaccgca accatgctgc cggttattct ggccgtcggt
     6961 aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc tgctggtgct gtctatcggt
     7021 atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg tgattattta cggctgtggc
     7081 tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct tcggggtgat ttacatctct
     7141 atgttgctgt tggttggctg gccgattctc gccatgtgga actaatacgt tcgccaaacg
     7201 ctgaataaat catcacgccc gccaggtaag cccacctggc gggctttttt atgatttaat
     7261 agatagtatt cagtgtatgt atgagttcca cacccattat gaaagcattc tgccgtaacg
     7321 ccgcgttgct cgcggtttct ctgcttccct tatcttctgc caacgccgta gcgttgcagg
     7381 caaaacagta tggcgatttt gatcgctatg tcctggccct ctcctggcaa accggatttt
     7441 gccagagtca acacgatcga aatcgtaacg aacgagatga atgtcgcctg caaaccgaaa
     7501 cgaccaacaa aactgatttt ctgaccgtac atggtctgtg gccaggattg cctaaatcgg
     7561 ttgctgcccg cggtgttgat gaacgccgct ggatgcgctt cggttgcgct actcgcccaa
     7621 tcccgaatct accagaagca cgcgccagcc gaatgtgttc atcgccggaa accgggttat
     7681 cactggaaac ggccgctaaa ctaagtgaag tcatgccagg agctggcgga cgttcctgcc
     7741 tggaacgcta cgaatatgcc aaacacggtg cctgctttgg ttttgatccg gacgcatact
     7801 tcggtacgat ggtacgcctg aatcaagaaa ttaaagagag cgaagccgga aaattccttg
     7861 cggataatta cggtaaaaca gtgagccgcc gtgactttga cgccgccttt gccaaaagct
     7921 ggggaaaaga gaacgtgaaa gcagttaagc taacgtgcca gggtaaccct gcgtatttga
     7981 ctgaaattca gatctcgatc aaagctgacg ccatcaacgc tccgctttct gcaaactcat
     8041 tcttgccaca acctcaccca ggtaactgtg gcaaaacctt tgtgattgat aaagcgggtt
     8101 attaatcctc tgatttacaa aacacgctaa ttcgtcttcc ataaccatgc cgattaagaa
     8161 ttatacataa tgcgctgcat tgaggaattc cctgaatgtg acgcaaatca cttcaaagag
     8221 tgagcaacag tcagtatcat catgtgagtt aaaccctcgc cgcctgacgg tgagggtttt
     8281 cttttgggta tgtttatctg cgacactcgc agtaccgacg acatggagta aaaatgtcca
     8341 gaccaactat catcattaac gacctggatg ccgaacgcat cgatattctg ctggagcaac
     8401 ccgcctatgc tggtttgcca atcgccgacg cgttaaacgc agagttggat cgcgcccaaa
     8461 tgtgttcgcc agaagagatg ccacacgacg tggtgacaat gaacagccgg gttaaattcc
     8521 gcaatcttag cgatggcgaa gtgcgtgtgc gcacgctggt gtatccggca aaaatgaccg
     8581 atagcaatac tcagctttcc gttatggctc cggtaggtgc cgcactgctg gggctgcgcg
     8641 ttggcgattc cattcactgg gaacttccgg gcggcgttgc aacccacctt gaagtgctgg
     8701 aactcgaata ccagccagaa gctgctggcg actacctgct ttaatcccat ctgcacaagc
     8761 gataactcgc tttgcccaga ggatgcactc gcatcctctt tacgcaaaat cagcaatttt
     8821 tcctggttct cactgttcac ttttgctgca aaattattcc gtttcgtttg tgggaataac
     8881 catggcagaa gcattttaca tcctgatagg atttctgatt atggcggcga ttatcgtcat
     8941 ggctgtcctt tacctcgaaa accactcctg acgatcatat tgttcccccc ggcatcgcat
     9001 tcaccagacc tgaaaccgcc ttctgcgccg cctctgcgct ttgtgcaccg ggtaccagaa
     9061 tcaccttacg gcactcctct tcacgttttt cgaaaatctc atatccccgg gcggcctctt
     9121 caaacggcat atagtgggta acaatttctt ctggtttcag taatcctttc tcaattaacg
     9181 gtaataattc tcccagccat gcgtgaacgt gggtctgtcc cattttaaac gtcacccctt
     9241 tatcaaaggc gtcgccaaac aggaaaccgt gaataaatcc agcgtagacg cccggtacgc
     9301 taacaatgcc gccacgcctg accgccgcaa tacactggcg caacgcttta ccgctgctgc
     9361 cctccagttt caggttagtc agcaccgttt ccgtggtgct gcctttcgct tcaaaaccga
     9421 cggcgtctat tactgcatcc acgccccggt gacctgccgt ttgttcaata attgactgtg
     9481 ccggatcgct atcttcatcg aaattaatcg ggatcgcgcc gtagcggtca gcggcgaaac
     9541 gcaagcggta gggatgatga tcaacaacaa aaatctgttc cgcaccgagc aaccgtgcac
     9601 aggcgattgt caacaatccc acaggaccag caccatagac tgcaacgctt gaaccttgtt
     9661 ggatctgcac attttttgct gcctgccatg ccgttggcag aatatcagaa aggaaaaggg
     9721 ctttatcatc tgaaagcaaa ggcggtactt taaacggccc cacattccct ttcgggacgc
     9781 ggacatattc cgcctgcccg ccaggaacgc caccatacag gtgactataa ccaaacaatg
     9841 ccgcgggagc aggtatctgt tttttattga gcgcagcgcc tttacccgca ttggtatttt
     9901 cgcaggcggc atattgctgt aatcgacaga aaaaacagtc gccacaagca atgacgaacg
     9961 gaattaccac ccggtcgcct ttttgcaaat tttttacgtc cttcccggtt tcaacgactt
    10021 cccccataaa ttcatgacca aaaatatcgc catgtttaac ctggggtatt ttgcctcgat
    10081 aaagatggag gtcagagcca cagatcgccg ttaccgtaat acgcagaata atatcatctg
    10141 cctgttcaat gcccggatcg ggaacatttt ctacctgaac gtgatgtggg ccgtgataag
    10201 tcaatgcttt catgcaacct ccagagacag ttcaaaaaag agtcagtctt taagggtagc
    10261 tatcacagcg ggaaaacggg cggtaccgca ggttttggga gaattcctag tgttcagtga
    10321 gaatatatta atcacagaaa ccgttcgtaa ggaaaaagaa ccagacaaat atcagggata
    10381 aatattataa tcatggagaa tgatttgttc atgattaaca gggagaaagg gttatgtata
    10441 aggcaatcat tatgccagtt gatgtatttg aaatggaatt gagcgacaaa gctgttcgcc
    10501 acgctgaatt cctcgcccag gatgacggag ttattcatct acttcacgta ctacccgggt
    10561 cagccagcct gagcctgcac cgttttgccg ctgatgtgcg tcgttttgaa gagcatctgc
    10621 aacatgaagc agaagaacgt ctgcaaacga tggtcagcca cttcaccatc gatccttccc
    10681 gcattaaaca acatgtccgt tttggtagcg tgcgggatga agtcaatgag ttggcagaag
    10741 aactgggggc tgatgttgta gttatcggtt ctcgcaaccc atcgatttcg acccatctgt
    10801 taggttctaa cgcctcgagc gtgatccgcc acgccaatct gcctgtactg gtcgttcgct
    10861 aacagataaa agtatttatg ccgccctgcc tggaacatcc cggcagggct ttttaatgcc
    10921 ataaaaaagc cgccaggttt gaccccggcg gctaagcaat tgcaggtgaa tcttacttct
    10981 tcttatgcag ttttggtgcg aatcaggtag tcaaaagcac tcagagaggc tttggcacct
    11041 tcgccagtgg cgatgatgat ctgcttgtac ggaaccgtcg tacagtcacc cgctgcgaac
    11101 acgcctttga cgttggtttc acatttcgca tcaatgataa tctcgcccat gcggttacgt
    11161 tcgactgcgc cttccagcca gttggtgttc ggcagcagac caatctggac aaaaataccg
    11221 gccagttcga tgttgtgaat attgccgctg acacgatctc gatattccag accaacgact
    11281 ttgctgccgt cgcctttcac ttccgtggtt tgcgcattca gaataatgtc gacgtttttc
    11341 aggctgcgca gtttgtcctg cagaacctgg tcggctttca tttctggcgc aaattccagc
    11401 agcgttacgt gctcaacgat acccgccagg tcgattgccg cttccacgcc ggagttaccg
    11461 ccgccgataa ccgctacgcg tttgccttta aacagcgggc cgtcgcagtg cgggcagtag
    11521 gtcacgcctt tagtgcgata ctgatcttcg cccggaacgt tcatgttgcg ccattttgca
    11581 cccgtcgcca cgataatgct gcgtgctttc agtaccgcgc cagaagctgt ttcaatctgg
    11641 tgtagaccac cttcaaccgc tgccgggatc agtttgctgg cgctctggct gtcgatcaca
    11701 tcaacgtcgt attcatcaac gtgaactttc agtgcgcctg ccaacttctg cccttcagtc
    11761 ttcggtacag aaatgtagtt ttcgatatca acggtatcga ggatctgacc accaaaacgt
    11821 tcgcccatca gaccggtacg gatgccttta cgtgcggagt aaattgctgc cgctgcaccc
    11881 gccgggccgg aaccaacgat taatacgtca taagcatcac gcttgttcag ctcttctgcc
    11941 gcacgttttt ccgcgccagt atcaattttg gcaacgattt cagtcaacgt catgcggccc
    12001 tgaccaaact ctttcccgtt tacgaacact gccggaacgc ccatcacgtt gcgatcggtg
    12061 atttcgttct ggaaggtgcc gccgtcaatt gcagtgtgct tgatgcgcgg gttcaatacg
    12121 ctcatcaggt tcagcgcctg caccacgtcc gggcagttat ggcaagagag cgagtaatag
    12181 gtttcgaatt caaaatcacc gtcaatatgg cgaatctgct ccagcagaga ctgcgcttct
    12241 ttcgacggat gaccaccggt ccacagcaac gccagtacca gcgaggtgaa ctcgtggccc
    12301 agcggggagc ctgcaaaacg tggtccctgg ttggaacctg ggttggtgat caggaaagac
    12361 ggcttacgca ctggcaagct gttatcttct ttaaaggtga ctttgtctga cagttctgcg
    12421 atttcagcca acagttcctt gatttctgcc gatttagcgc tgtcatccag cgtggcaatt
    12481 aactcaacag gcttggtcaa tttctcaagg taagccttga gttgagtttt catatttgtg
    12541 tcgagcatgt ttatctcctg ggctttaaac atcatcatgc aagcggcctc tgcggctacc
    12601 tggatgcagc ttgcatcatg gtgcggggaa gaaaatcggg tgccgcagca cccgaagaat
    12661 taggtgaatt tccgaagtat ttcgcgtcag agcaaggcga ctagcctgtg aatccccggg
    12721 agcttacaca agtaagtgac cggggtgagc gggcgacgcc aacgccgcta tggcgtgaaa
    12781 gacgaaggaa atttagattt taccaaccag gtccagagac ggagccagag ttgcttcacc
    12841 ttctttccat ttagccgggc aaacttcacc tgggtgagaa gctacgtact gagctgcttt
    12901 gattttacgc agcagatcag acgcgtcacg gccaatgcct tcagcggtaa cttcgattgc
    12961 ctggatgata ccctgcgggt caacaacgaa ggtcgcacgg tcagccagac cttcatcttc
    13021 acgcatgttg tcgaagttac gggtcagggc gccagtcggg tcgccgatca tcgcatattt
    13081 gattttagcg atggtttcag agctgctgtg ccatgctttg tgggtgaagt gagtatcggt
    13141 agatactgcg tatacgtcta cgcccagttt ctgcagttct tcgtagtggt cagcaacgtc
    13201 acccagttcg gtcgggcata cgaaagtaaa gtcagccggg tagaagaaga agacgctcca
    13261 gcggccttcg gtatcttttt cggtgatttc gatgaattcg ccgtttttga atgcctggtt
    13321 tttaaaaggt ttaattttgg tgttaatcaa ggacatctat acttcctccg tgttttcgat
    13381 gagatgtaag gtaaccaatt tttgccgatt cggctaatgc gtttccatta tcaaatcgat
    13441 aagttttacc ttacaacctt cgtaagacaa ctttgtgaac tttgcaccgc cagcggcgat
    13501 aaaaagtaaa atggccccct ttccaggtga ccgtgtaatc tgaactaccc tttggtatct
    13561 tcagcgcctg cctgcaggct gaggtgttgc ccttacaaat gcaacaacga catggattac
    13621 aacaccctca taaacaaagg gcaatcacct gatctaagct cttacctatg acagtgatag
    13681 gttatgcctt ttactcgact tttgcactga ctgaaaagaa caaattaatg ttaaaaaaga
    13741 tacttttact ggctctgctt cctgcaatcg ccttcgcaga ggaacttcct tccccagtaa
    13801 aagcgattga aaaacagggc attacaatca tcaaaacgtt cgatgccccc ggaggaatga
    13861 aaggttatct cggaaagtat caggatatgg gcgtcaccat ctacctgact cccgatggta
    13921 agcacgctat ctctggttac atgtacaacg agaaaggtga aaacctgagt aacacactta
    13981 tcgaaaaaga aatttacgca ccagccggac gcgaaatgtg gcaacggatg gaacaatccc
    14041 actggctcct cgacggtaaa aaagatgcgc cggtcattgt ctacgtcttc tccgatccgt
    14101 tctgcccata ttgtaaacag ttctggcagc aggcgcgccc gtgggtagat tctggcaaag
    14161 tgcaattaag aacattgttg gttggggtta tcaagccaga aagcccggcg acagcagcgg
    14221 caattcttgc ctccaaagat cccgcaaaaa cctggcaaca atatgaagcc tctggttgca
    14281 agcttaagct aaacgtgcct gcaaacgtaa gtacagagca aatgaaagtg ttaagtgaca
    14341 atgagaaact gatggacgat ctgggggcaa atgtcacgcc ggctatctat tacatgagta
    14401 aggaaaatac gctgcaacag gccgtggggt tgcccgatca gaaaacgctt aatatcatta
    14461 tgggaaataa ataaaaggta attcatggat ggttgaacta tatccctgct tttcaaccat
    14521 cctaaaaaag catgaatttc tcgctctacc ctaagtggct gtgctatttt agaaaaactt
    14581 aatcctgagt taagcaaaaa aacataatca ataaaaatac atacgcaaaa aacattgatt
    14641 aagtgaatat accatggaag aaaaatataa accggagtag tgtatggcca atctctacga
    14701 cttgaaaaag ttcgacttaa atcttcttgt catatttgag tgtatttacc agcatttaag
    14761 cattagcaaa gctgctgaat cactatatat caccccatca gccgtgagcc agtcgttaca
    14821 acgcttacgt gcgcaattta atgacccttt attcattcgc tcaggaaaag gtatcgcgcc
    14881 gacgacaaca gggttgaatc tgcatcacca tcttgagaaa aacctcaaag gccttgaaca
    14941 aacaattaat atagtcaata agtccgaact aaagaaaaac tttattatct acggccctca
    15001 gctcatttct tgctctaata atagtatgct gatccgctgc ctacggcaag atgcctctgt
    15061 cgagattgag tgccatgata tcctcatgtc agcagagaat gctgaagaac tgttggttca
    15121 ccgcaaagcc gatctggtga tcacccaaat gccagtgatc agccgttccg tcatttgcat
    15181 gccattgcat acaattcgca atactctaat ttgtagtaat aagcatccgc gaattactga
    15241 caatagcact tatgaacaaa ttatggcaga agagttcacc caattaatag ccaaatcagc
    15301 aggagttgat gatattcaaa tggaaataga cgagaagttt atgaatcgca aaatttcatt
    15361 tcgtggttcc tctttgctga caataattaa tagcattgca gttacagatt tactgggaat
    15421 agtcccctat gaactttata actaccaccg tgactttctt aatttaaaag agataaaact
    15481 agaatatccg ttaccttcaa taaagcttta catttcctat aataaatctt ctttaaataa
    15541 tttggtgttc tcccgattta ttgaccgtct aaatgagagt ttttgatata taactataga
    15601 cagctactat aactttcatc tatttattca cagcgcgaat gaaaaataaa acactctgct
    15661 ttacagtgtt tattttataa ttaacagtct tatctcactg ttcaccctgt aacgttcaga
    15721 gagtgtcttc agatgtctat ttataaaatt cctcttccgc tcaatattct ggaagctgca
    15781 cgagagcgta ttacctagac actcaacacc ctaccccgcg tttgtgtctc attttctggc
    15841 ggcaaagact ctggtttaat gctgcatctg acagccgaac ttgcccgaca aatgggcaaa
    15901 aaaatctgcg ttttgtttat caactgggag gc
//
LOCUS       DAOFMX010000088        15409 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.088, whole genome shotgun sequence.
ACCESSION   DAOFMX010000088 DAOFMX010000000
VERSION     DAOFMX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.088"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..1175)
                     /locus_tag="QBA20_003319"
     CDS             complement(<1..1175)
                     /locus_tag="QBA20_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDQ6293743.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="QBA20_003320"
     tRNA            complement(1374..1458)
                     /locus_tag="QBA20_003320"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="QBA20_003321"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="QBA20_003321"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDQ6293744.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="QBA20_003322"
     CDS             2802..4304
                     /locus_tag="QBA20_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDQ6293745.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="QBA20_003323"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="QBA20_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDQ6293746.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="QBA20_003324"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="QBA20_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDQ6293747.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="QBA20_003325"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="QBA20_003325"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDQ6293748.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="QBA20_003326"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="QBA20_003326"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDQ6293749.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="QBA20_003327"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="QBA20_003327"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDQ6293750.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..13065)
                     /locus_tag="QBA20_003328"
     CDS             complement(11869..13065)
                     /locus_tag="QBA20_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HDQ6293751.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13258..13761
                     /locus_tag="QBA20_003329"
     CDS             13258..13761
                     /locus_tag="QBA20_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ6293752.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="QBA20_003330"
     CDS             complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="QBA20_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDQ6293753.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14385..15398
                     /gene="argF"
                     /locus_tag="QBA20_003331"
     CDS             14385..15398
                     /gene="argF"
                     /locus_tag="QBA20_003331"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDQ6293754.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3975 a         3931 c         3965 g         3538 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgttgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctatttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gccagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcctaaa caacaccaga gaaggcccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgta
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgttcgtc cagctcggta atcgtgttca gcacgctttg cgggctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatcg ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtctggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcagt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatgg cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8221 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcagtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcaccccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc tgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttatcga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag ccctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc ataactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttta gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12301 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaag gtaataaagc
    12361 taaaaatgcg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12421 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12481 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12541 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12601 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12661 aaaactaaat cttacgccgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12721 tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa caagcggcat
    12781 atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12841 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12901 ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat
    12961 cgttaacaac acattcacca cacaaataag aaaatatctc tcacctgtac catgaaaaac
    13021 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    13081 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    13141 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    13201 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13261 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13321 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13381 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13441 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13501 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13561 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga gatgggcttc
    13621 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13681 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13741 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13801 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13861 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13921 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13981 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    14041 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    14101 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    14161 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    14221 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14281 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat aatacacata
    14341 aattgaattt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14401 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14461 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14521 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14581 ccgcatatga ccagggggct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14641 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14701 atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat
    14761 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14821 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14881 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttacgtc
    14941 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    15001 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    15061 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    15121 aacgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    15181 acccggaggt caaattcctc cattgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15241 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15301 tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15361 cggtgatggt cgcgacgctc agtaaattaa ataattaata tctctataa
//
LOCUS       DAOFMX010000089        15347 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.089, whole genome shotgun sequence.
ACCESSION   DAOFMX010000089 DAOFMX010000000
VERSION     DAOFMX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.089"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(173..1198)
                     /gene="tdh"
                     /locus_tag="QBA20_003332"
     CDS             complement(173..1198)
                     /gene="tdh"
                     /locus_tag="QBA20_003332"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDQ6293755.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="QBA20_003333"
     CDS             complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="QBA20_003333"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDQ6293756.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="QBA20_003334"
     CDS             complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="QBA20_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HDQ6293757.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3840..4772
                     /gene="rfaD"
                     /locus_tag="QBA20_003335"
     CDS             3840..4772
                     /gene="rfaD"
                     /locus_tag="QBA20_003335"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDQ6293758.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4782..5828
                     /gene="rfaF"
                     /locus_tag="QBA20_003336"
     CDS             4782..5828
                     /gene="rfaF"
                     /locus_tag="QBA20_003336"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDQ6293759.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5832..6824
                     /gene="rfaC"
                     /locus_tag="QBA20_003337"
     CDS             5832..6824
                     /gene="rfaC"
                     /locus_tag="QBA20_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDQ6293760.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYTIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6821..8029
                     /gene="rfaL"
                     /locus_tag="QBA20_003338"
     CDS             6821..8029
                     /gene="rfaL"
                     /locus_tag="QBA20_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HDQ6293761.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8065..9207)
                     /locus_tag="QBA20_003339"
     CDS             complement(8065..9207)
                     /locus_tag="QBA20_003339"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HDQ6293762.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9216..10229)
                     /locus_tag="QBA20_003340"
     CDS             complement(9216..10229)
                     /locus_tag="QBA20_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HDQ6293763.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="QBA20_003341"
     CDS             complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="QBA20_003341"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HDQ6293764.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="QBA20_003342"
                     /gene_synonym="rfaI"
     CDS             complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="QBA20_003342"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HDQ6293765.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="QBA20_003343"
     CDS             complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="QBA20_003343"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDQ6293766.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12827..13951)
                     /locus_tag="QBA20_003344"
     CDS             complement(12827..13951)
                     /locus_tag="QBA20_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDQ6293767.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="QBA20_003345"
     CDS             complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="QBA20_003345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDQ6293768.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4382 a         3448 c         3144 g         4373 t
ORIGIN      
        1 ctgtgagata ttatttttac aggtgagaga gtcacttcag ggtagagaga taatgagaac
       61 agctatataa aagatcagcg caattacgaa ctctttatta cactgacagg attttacata
      121 attaacagat tatcctcaga taaaagggct ggaataccag cccttgttcg tgttaatccc
      181 agctcagaat aactttcccg gactggcccg aacgcatagc gtcaaagccc ttctggaaat
      241 catcgataga gaaacgatgg gtaatgatcg gcgagagatc gaggccagac tgaatcagcg
      301 ccgccatctt gtaccaggtt tcaaacatct cacgaccgta aatgccttta atgaacaagc
      361 ctttaaagat cactttggtc cagtcgatag acatatcaga cggtggaata cccagcatcg
      421 caatacgacc gccgtggttc atggtatcga gcatggtacg aaacgctggc ggcgcgccgg
      481 acatttccag accgacatca aagccttcgg tcatgcccag ttcagccatc acatcattaa
      541 ggttttcttt cgcgacgtta accgcacggg tgatacccat tttgcgcgcc agctcaaggc
      601 ggtattcgtt aacatcggtg atcaccacat tgcgtgcacc aacgtgtttc gccaccgccg
      661 cagccataat acctatcgga cctgcaccag aaaccagcac atcttcgccc accagatcga
      721 acgacagcgc ggtatgcacg gcgttaccga agggatcaaa aatggaagcc aggtcgtcgg
      781 agatattgtc ggggattttg aaagcgttga acgccggaat caccagatat tcggcgaagc
      841 agcccggacg gttaacaccg acaccaatgg tgttgcggca cagatgggta cgaccgccac
      901 gacagttgcg acagtgaccg caggtgatat ggccttcgcc agaaacgcga tcgccgattt
      961 tgaagccttt cacttcctga ccaataccta ccacttcacc gacatattca tggcccacga
     1021 ccatcggcac cgggatggtt ttttgcgacc actcatccca gttatagatg tgaacgtcag
     1081 tcccgcagat ggctgtttta cggattttaa tcagcagatc gttatgcccg agttccggta
     1141 cagggacgtc ggtcatccag atgccctctt ccgctttcag tttggataac gctttcatct
     1201 cacatcctca ggcgataacg cccagttgtt taccaatacg cgtgaatgct tctactgcac
     1261 gcgtaatttg ctcaggggta tgcgccgcag acatctgggt acgaatacgc gcctgacctt
     1321 tcggaacgac cggatagaag aaaccggtga cgtaaatgcc ctctttttgc agctctcggg
     1381 caaatttctg cgccactacc gcatcaccaa gcatgaccgg aataatggcg tgatcggctc
     1441 ccgccagggt aaagcccgcc gccgacattt gctcacggaa ctgacgcgcg ttcgcccaca
     1501 gacggtcacg caactcgcta cccgcttcaa ccatctccag cactttgatg gaagcggcga
     1561 caattgctgg tgccagcgag ttggagaaca ggtacggacg agaacgctgc cgcagccact
     1621 caaccacttc tttgcgcgcc gcggtataac caccagaagc cccgcccagc gctttaccga
     1681 gcgtgccggt gatgatgtcg acacgcccca tcacatcgca gtattcatgg gaaccacgac
     1741 cattttcacc gacaaaaccg accgcgtggg agtcgtctac catcaccagg gcatcgtatt
     1801 tatctgccag atcgcaaacg cccttcagat tggcaatcac gccgtccatc gagaacacac
     1861 catcggtggc aatcagcaca tgacgcgcac cggcttcacg cgcttctttc agacgagctt
     1921 caagctcctg catatcgttg ttggcatagc gatagcgttt agctttacac agacgcacac
     1981 catcaataat agacgcgtgg ttcagtgcgt cggagataat ggcgtcttcc gcgcccagca
     2041 gcgtttcaaa caggccaccg ttagcatcaa agcaggaaga gtagagaatc gcatcttcca
     2101 tccccaggaa tgccgccagt ttttgttcaa gctctttatg gctgtcctga gtgccgcaaa
     2161 taaaacgcac cgaagccatg ccgaaaccgt gagaatccat tcccgccttt gctgccgcaa
     2221 tcagatccgg atgattcgcc agcccgagat agttgttggc acaaaagtta atgacgtggc
     2281 ttccatcagc cacggtgata tctgcttgct gcgcagacgt aataatgcgc tcttctttaa
     2341 acaacccttc cgcccgtgcg gtttccagat cgttggttaa ctgctgataa aattctccac
     2401 gcattgcgat tctccagaca gggcaaattc cagcacatat tacccaaact tataggtcgc
     2461 gacgagatga tgcgttaaca cttctgcaaa attcaggata aataacgtgt atcttgatgt
     2521 ctctcaacac ctacagatag tagaaaaact gtacagtttt ggctgagcat aatgatgagt
     2581 gattttactg cattgatata aaaagaaaaa atgatgccaa agcatcattt ttcctatctc
     2641 agttggcatc aaaacgctac ccactaatgg agggaaacct attttcttga aaggaaaatt
     2701 ctcagcgcct gcatttttgc ccctaaagtg ttactcctga agcagcgaaa caaatcgaac
     2761 cattttcctc tccccagata gtttaggttg aagagatcgg aacgtttgta atagcacatg
     2821 acaaaaatgc cctggtcatc gtccacaatg ttattgttta gtgttatttt ttgactttcc
     2881 agaactaatt tatagaattc tttccattta tgctgtgaac caacaattgc gcccccaata
     2941 atatatacat gattgccaat catgaaatca aaagcctgtt gttgagatgt aacagtaagc
     3001 ccttttttta tagtgaaaag atgcatctta ttttcatcaa atgggaaatc ccaaattttc
     3061 aatcctcgag ttacatttgg cttacggcaa tagccaaaat ctatccacgc aacaagaggc
     3121 gttttcacta aacccatgtt gatagcttta tttacaaaat aggccttaag gttacatacc
     3181 aaaacatact ctggtgacca gtactctggg ttttttaatt gtcgaggctc aagtctattt
     3241 gtaaatgact catctttttg aattttttcg atacgacttc tgatatatct aaatttcttt
     3301 tttatatcga taacgataac cgttgttggt tttccgttgc gaatcgcttc aatccttggt
     3361 ttcaaatcgg gagaagtgaa aataatcatc tcattttcaa gtgcggccag acgctcaaag
     3421 taactaaaat aaacatcaac tgaacgagct agtttttcac ggaacccttt attagcagtc
     3481 caatcccctc taccaatgtc aaaatatgca gtgataattg tggtggatga tttcattact
     3541 aataagacca ttaatataaa aaaagaatta tatcataaat attaacagaa tgtatactgt
     3601 atattatcca ttcaaatgat tccgttattt atcatacatc ggaatattga tactaaagca
     3661 ctattcacat gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg
     3721 ttgcaattag catccttgca cctctatgta aagggctgaa gggattcgga tgtgatggta
     3781 tgattacaga cattcgtgtc tgagattgtc tctgactcca taattcgaag gttacagtta
     3841 tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaag gccctgaatg
     3901 ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga
     3961 acctggtgga tctggatatc gcggactata tggataagga agacttcctg atccagatta
     4021 tggctggcga agagttcggt gatgtcgaag cgattttcca cgaaggcgca tgctcttcca
     4081 ccaccgagtg ggatggcaag tatatgatgg ataacaacta tcaatactcc aaagagctgc
     4141 tgcactactg tctggagcgc gaaatcccgt tcctgtatgc ctcttccgca gccacctacg
     4201 gcggacgcac ctccgacttt attgaatccc gcgagtacga aaaaccgttg aatgtctacg
     4261 gttactcaaa attcctgttt gatgaatatg ttcgtcaaat cctgccagaa gcgaactcgc
     4321 agattgttgg cttccgctat ttcaacgttt atggaccgcg tgaaggccat aaaggcagca
     4381 tggcgagcgt cgctttccat ctcaacaccc agcttaataa cggtgaatca ccgaagctgt
     4441 tcgaaggtag cgagaacttc aaacgtgact tcgtctatgt gggcgacgtg gcagatgtaa
     4501 acctgtggtt cctggaaaat ggcgtttccg gtatcttcaa cctcggcacc ggtcgtgcag
     4561 aatccttcca ggcagtagca gatgctacgc tggcttatca caagaaaggc cagatcgaat
     4621 acattccgtt cccggataag ctgaaaggcc gctaccaggc gttcactcag gcagatctga
     4681 caaatctgcg cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg
     4741 aatacatggc ctggctgaat cgcgacgcat aagagctctg catgaaaata ctggtgatcg
     4801 gcccgtcttg ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc
     4861 gctatcccca ggcgataatc gatgtgatgg caccggcatg gtgccgtcca ttattatcgc
     4921 ggatgccgga agttaacgaa gctatcccta tgcctctcgg tcacggagcg ctggaaatcg
     4981 gcgaacgccg caaactgggt catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct
     5041 taccaaactc cttcaaatct gcattagtgc ctttcttcgc gggtattcct catcgcaccg
     5101 gctggcgcgg cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag
     5161 cctggccgct aatggtggaa cgctatgtcg cgctggccta tgacaaaggc attatgcgta
     5221 ccgcacaaga tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa
     5281 aatcatatac ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt ggcttttgcc
     5341 cgggtgcgga gtttggtccg gcaaaacgct ggccacacta ccactatgcg gaactggcaa
     5401 agcagctgat tgatgaaggt tatcaggtgg ttctgtttgg ctctgcgaaa gatcatgaag
     5461 cgggcaatga gattcttgcc gctttgaata ccgagcagca ggcatggtgt cggaacctgg
     5521 cgggggaaac acagcttgat caagcggtta tcttgattgc agcctgtaaa gccattgtca
     5581 ctaacgattc tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt
     5641 atggtccaag tagcccggac ttcacaccgc cgctatccca taaagcgcgc gtgatccgtc
     5701 tgattaccgg ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat caccagagct
     5761 tgatcgacat tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg
     5821 aagcctgacg gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat gttctccata
     5881 cgttgcccgc actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg
     5941 tggaagaagg gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg
     6001 tggcaatacg tcgctggcgt aaagcctggt tctcggcccc cataaaagct gaacgcaaag
     6061 cgtttcgtga agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg
     6121 taaaaagcgc ggcactggtg acacgtctgg cgcatggcgt aaagcatgga ttggactggc
     6181 aaaccgctcg cgaaccttta gccagcctgt tttacaattg taagcatcat attgcaaaac
     6241 agcagcacgc cgtagaacgc acccgcgaac tgtttgccaa aagtttgggc tatagcaaac
     6301 cgcaaaccca gggcgattat actatcgcac agcattttct gacgaacctg cctacagatg
     6361 ctggcgaata tgccgtattt cttcatgcga cgacccgtga tgataaacac tggccggaag
     6421 aacactggcg agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt
     6481 ggggcgcgcc gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg
     6541 aagtattgcc gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg
     6601 tagtgtcggt ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca
     6661 cggtttatgg accaaccgat ccgggattaa ttggtgggta tgggaagaat caagtagagt
     6721 gtcgttctac aagtatgtct cttgcagatt tgccagctca aaccgttttt caaaatttaa
     6781 atcttgaaat aataaccaat aagttgacat cggagataag atgacctcaa cattattttt
     6841 ctctctcgag aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttattcat
     6901 taccacctat tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac
     6961 aataacgaca atcgtctatc tctgcaaacg gccaaaacac tatctctcac tatttaaaac
     7021 atttcttttt ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc
     7081 ccctgatgca ggtgctagca tgaaggaagt tttcaaagct attattgaga atactttact
     7141 atgcacaata gcaataccgg tcatattgag agacgagaaa agagaagatg tcgaaaaaat
     7201 cgttttcttc tcatttatta gtgcgttggg cttacgctgt ttttctgaat tgattaccta
     7261 ttataaggac tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc
     7321 tgactcgatg gtctttttat tccctgcatt gttaaatctc tggcttatca aatcagcaaa
     7381 ataccgcatt tcttttgtgg ttctaagcgt tatttttatt tttctgatat taggaacttt
     7441 atccagaggg gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa
     7501 acaatggaag ttactattag taggagtaat ggtttccatc attgcattgt cggttatttt
     7561 cacacataag gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta
     7621 tcgctatgca aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat
     7681 tggttatggt tacggtaacg ttgcatataa agatgtctat aataaacgtg tcattgatta
     7741 tccagaatgg acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt
     7801 tggcactggt ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga
     7861 gtgtataaaa aatggcgtca agaataaata tcgctcacca tataatgcat attatataat
     7921 cttactatct tttataggtt attttgttat ccgtggaaac gtagaacaaa ttgaaccaaa
     7981 tttattaggc gtttacgccg gcttattatt agcgatgaaa aacaagtaaa aaataaaaaa
     8041 ggctgcataa tgcggccttt ttatttattc aaaccaatta tgaataacct cttcgaatct
     8101 ttgagttacg ccatcccaac tgtagtgatc aaacacaaag tcctgacctt gctttgcaac
     8161 tgctgttaat tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg agtctgcagt
     8221 cataggttct tttaaatgga atcccgtagt attttctttg acaaattccg tcatcccccc
     8281 acgcgtactc actaaaacag gcttaccagc acccatagct tcaattgcca ccatgcaaaa
     8341 tggttcctgg aactgggaag gaatgacgac taaatctgcc agtgggtaat aacaatacat
     8401 cttttcaggc ggtatgcttc ctaacataat gcaacgatcc ttcaaacgtt ttgcaatttc
     8461 cctgacattg cgttgatatg cacctttgtc acttttactc atttctgtat aatcaccaat
     8521 cacaactaat tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt gcatcaacag
     8581 caatatgcct ttgtcaggga cgatacgccc agcataaaaa ataattttct cttcaggaga
     8641 gatattaagt tcgcttctag tgatcggctg aaaattagat tgataggttt ctaaatcaat
     8701 tccatttggt acaatctcaa tatctgcatt tgctaaataa gattggtaat actttttcaa
     8761 gtataaacta gggactatca ttttaacatt ttgctcaagc aattttggtt caaatgcatt
     8821 atgcatatgt ataaccactc ttgcctgagg agctagctta cgaatttgag tgtatagctt
     8881 catgctatta tgaactacaa taacactttc tttcgttata ggaaaatcat gtgcaatatt
     8941 caatattctc tgtgaatatg gaagaggatc aagtcttgtc catttttgaa ataaccgctt
     9001 atatatgcga ctgaatccaa ccctatgaat actacaatta tcattaacaa aggtgtaatt
     9061 tgagtatcct ggatttttga tacaaacaat acgatttgga aaattggtac gctgagcaac
     9121 ctgatacatc cacgtttcaa cagcagccgc tccgcgagga ggaattgaaa aaataggagt
     9181 aaccgtaaat attattttat caaccatctc gtatattaac ctttcataac attatattta
     9241 attgctgtaa aaacaccatc gagaaatttt ttctggtaaa gcaagtgttt atatttttct
     9301 ttgtgctcat gttttttaac agctttttta taaggtatat ttctccatgg tgatatatta
     9361 taaatattac gaaaataatc tgcagaggcg tagttcgccc aatcatgcca tggcttagtt
     9421 ataccagtat aatgtatgaa gacagtgtca tcattaataa accgggtgta atattcacta
     9481 tttttttctt caaattctga ctttatagta taaatacaat tatattttct cggtaatatt
     9541 ttagcatgat gtagaaacat taagtttaaa atatcttgat ccgggtactt taattttttt
     9601 ataattgact catctgataa tagatcaaag aatttttccg ttagtctttg ttttaaccat
     9661 tcccgtaaat taatatacat tacaccagag ttaaaatagc taccgttaaa atcctcatta
     9721 cacaaacgct cagcgctttt actttgcata gcatctacat caattaccac tgccccatat
     9781 tcatctttaa actctaatgc tattaactcg ttcaatgaac ccttacagac gatatcagca
     9841 tcaagatata ataatgaatc caatcgcgca gaaaaataat caaatgaaag caaacgataa
     9901 taaatagata ctggccagat atttgattga ggtagcgcct tcaatggctc agaatcaata
     9961 tgatataatt taattactgt ttgatacttc gttgctaatt gtgataataa ttcaatatac
    10021 tctttgtcta agtaatcagc gattatgtga aaggtaaatt tgttttgttt attcttttca
    10081 agaattgaag caattgaaat cgcagcccca acctggtagt tctcatcaat accccatgag
    10141 acattaaacg tttcccgttc atcaagttta acggggcgct tgtccagttc aattatatct
    10201 ttaaattgag taagatgttt aaaatccaat ttaattaccc ttcatcataa tcattatttg
    10261 ctcttcaatc ctagtttgac ttttacatcc ctgattactt ttttaatttt ctttttgaat
    10321 ataagatatg tatagccaaa gtctttcaat tcatttttta tattatagtg ccgttcaaga
    10381 tcaatgcgat cttttgcttt taaaacagcg gttgtttttt tacctgaaag gtcaattagt
    10441 cttaaacctt tttcggatac tataaaattt cctttatgag gatcgccaga caccattcca
    10501 tgctgatgca actcttttat cgattctgat aattgatcct tcagatcttc agaaatttcc
    10561 aaatactcat ttaacccgac accttcaata tactcaatga gcataatata ataatgagcg
    10621 aaatttaatg ttttacgttc agctagcaaa aaatagtcat taattgattg gatgccttca
    10681 ccacgtaccc gatcggtttg ataaattaaa ttttcataat aatctttttt tatgcaagat
    10741 tttaagaaac gttcggtcat tttatgcttt ggcgcataaa ctttaagtac aagaggtcca
    10801 cgagcagtgt caataagaat gactttagta tcatcaatag aacgaaaaac ttttaatgtt
    10861 ttaaccctgc aagttaaaaa gtcatttaga acttgctcat aaaagggatc attatctttt
    10921 atgaaaactt tcaacccatt tatcgtttta ttatatatca taatgaatta ttttaacctt
    10981 aaatctttag aagcattttt ctttataata ctttaaataa ttaaaaatgc ctgccatata
    11041 atgcttttgt ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt
    11101 aagtaaatct tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgaaac
    11161 cggataatca gcccactcat gccagggttt tgtaggtccc acatagtgta taaacacggt
    11221 gtcatcattg actggattat caactttgtc ttttaattca tagttaatac tatatcgggt
    11281 attatatttc tccgaaataa acttcacttt accattcaat aatacattca gtacatcttg
    11341 atcaaggtgg gtgattttac ttacccaatc tgggtcacgc aacatttcaa tagcttttga
    11401 cgaaatgtta tttagattcc actcatcaat atttatcagt aaaaaaccag cattgaaata
    11461 tccagaagct aactgtggtg tagttaatac cgaggctcga ttttgccacc attcaatatc
    11521 tctttcagct acaactgcgg caatttcatt agtagaaaat tgataatcta agagttcttt
    11581 aatactaccc ttgcaagcaa tatctgcatc aagatatagt attttttcat gtttatgata
    11641 gaaataatct gcaattataa atcgaaaata tgttgcgtaa gtccagtttt tcgtgcttgg
    11701 taatgacttc agcttatcac aattgataac ataaatatta attcttgaat tatattgttt
    11761 tgctaaatca gaaaaatata atttgtcttt atcactgata taatctgtga aaacatgaaa
    11821 ttcacaagag atttctctat tgtttaatag aatagatgcg atggctacac cacatccaaa
    11881 aagaaaattt ctgtcaatac caaatgctat atcaagagta tttttagatc ttatattttg
    11941 ataatgaaaa ttatactcaa aaaggatgat gtcactatca ttgagttgag acatatttat
    12001 tctgtatcca aaattttggc aaaaataagt tacgggttac aacgattttc gaatcgttct
    12061 ttccctgatt tttgtggctt ttgcttctgc ttgcgacagc agtccttgtt cctgcttgag
    12121 aatgtcttta agcggggcgg caaaatacac cttcataaag cgccagatat cccgctgagt
    12181 caggccaata ttcattgaag aaaaataaag ccctataaga tctttatcgc gccaacgacc
    12241 tggaacgcgc gtgcgaagct gcgcccggtg taggtcaatt accgaaattt ttaactcctc
    12301 ttccttaccg gaaaaaggca agtgcagcag gaaatgacag atataacagt cacgatggtt
    12361 aatgcccgca gcatgcatat cgcgcaccat cgtcgcgaca cgcttaataa gcatacgctt
    12421 tacgcgaaca tctggtgggt tggtcgccca gtcagcacag taatcttcca gactgatggt
    12481 tggtgtcaga tcttcggtaa taataaacga ggttctggtc agcggattca tgcctttttc
    12541 gccgaatgcc accccataca tagtatcaac gccgacatcc cgcagtcgat gaatcgcatt
    12601 ccattcgcgg tctgcgccta atactggcat ccgcaatgag agtaaatttt tgattatctc
    12661 tttcagggtc gtgccgcgat gccatttgag aaaatagctt tttcccgcca tttcaaagcg
    12721 cagagtacgg cgagtttcca gttcacgaaa tacctcaccc tgcaaggttt taacttcctc
    12781 aaaaggatct ttgccgcgcc ataacgtggc aaacggctct ttaagttcaa ccatccagac
    12841 cacccgttat gatatccgcc gctttctctg gcagactgta taaatcttgt gtatcagcat
    12901 aatgtcgcgc attttccgcc caagcctggc gcaatgaaga ttgcgttaac gctttgcgta
    12961 aaatctcatt caatgtttcc tggcggaatg gctcagcaat agcctcgccg caattagcgt
    13021 cgacaatata atgcgcatag ccacaaacgg cagttgttag taccggtaat cctgcagtaa
    13081 tcgcttccag cagcacaatt cccgccgctt cctggtaggc aggatgcagt aataaatccg
    13141 ccgccgccat taattccgag acatcgttgc gccccgagaa gaagtgaaca ttactgcgca
    13201 cgccgcgttt ttctgccagt gcctcaaatt ttcgcggttt atcctgccca acaacatata
    13261 gcaatgtgtt gtggcgcagc gaatccggta acgaagcaag tgcttcaatg gaacgatcgg
    13321 cacctttacg cgtgaagtct gaaccgacct gcaacaataa atattgttgt tcggttattc
    13381 cattcttctt acggaagatt tcacggctat tggctggctg ctggctatat ttacgatcag
    13441 gataaatccc cggtggcaga atatgaaaac gctccgcttc agtctgataa tgtttctgga
    13501 aatcggcgat ttgcttatct gtcagcatca gcagctgtgt cggcttgccc tgttcgaagg
    13561 ttgcccgctc aaaggcggca taatggcgat aacgtgacgt caggcgatag aaaaagcctt
    13621 tttcctgcgc tactttctcg gcataacaaa catcagcggc ataataaacg tccagccccg
    13681 gcattttgtt gaatccaacg actttatcga cgggatgttc gcgtaaatgt ttttgcaccc
    13741 aggcaaaata ctccgcattg cgcccgtgat tggtatgcga tttaaccggc actttgatca
    13801 cttcaaatac atcagggcat tcgccttccc acgactgggt ataaacccga acatgatgac
    13861 ctcgggcggc gactgtctga gcaatacgca taaaatcgcg ctgcaaaccg ccaaagggaa
    13921 aatatttata taaacaaaaa gcaacgatca taacgacgta cctgtcgtgg aggagggcag
    13981 taatttatcg acagcagcaa tgacatctgc cgccggaata accgaaagat acatctcatt
    14041 tcggtcacgc tgatcgcgcg ttggcatttc ccggtaatct cccgcccaga attgaatcat
    14101 gttatttgac cagggacgcc agaaaatatg atctgttgcg ccaaacagcg atatcagcgg
    14161 cgtattaact gcagcggcaa tatgcgccgg tgcggaatca acgccaataa acagctgcgc
    14221 atgatcgatt aacgcaccaa gttccgggaa ggtcaccttt ccagccagcg ccgttactgg
    14281 tggcgtctgg catccctgcg caatttcatt gacgcaggcc agatcgtctt tatctgggcc
    14341 ggacgtcaga acgacttcat aaccacgagc atgtaaggca tcaatcacag cggaaaactt
    14401 ggcgttgtcc cagcatttga agatttgccg cgccgtaggt tggataacca cataattttg
    14461 tccaacacca gcgtgatcaa gttcgcgacg catacgtttc cagcttgcag gcgggtaact
    14521 catggttgtc tgcttcacca acgaatcaag tcccaatggt gtcagcacgg ataagttact
    14581 ttccaccaca tttccaccct gcaacggcac caaatgggtg aaacttttac gccaaaaagc
    14641 agactgccga tgatgataat cctgggaaat tttcacacga gcatttaata agcgaaccag
    14701 tatagcaacc atccattgat cggtaagatt gacgataagg tcatacttat tggcacgtaa
    14761 taccttgatg agatgaaaaa agttggcaat tttttctgag gcttttgctt ttttattttt
    14821 tatgccgtag agcgcgttaa tctctggatt ttcagacagg atcgggatgg tgtcctgata
    14881 aagcagcaca tcgatttttg cgtcagggta attttttttc agagaactaa tgacgggagt
    14941 agttaataac atatccccat gaaaacgcat cttaatgagc aaaattcttc gaaatggctt
    15001 atccacaagt aactcttttt gttgagatta tccgattaag gtgaagcaga aaaagcacgc
    15061 taccgcccca ggctcaacag ctacctgaat actgataccg cactgatatt gacgcgctag
    15121 tgtatcactt ctttcgcggc aatccgaccc gaatgatttt taagcacatt gttgattttc
    15181 aataaagaaa tcaatcaaat aaaatcatta gcaaccatat tctttcactg ttgctctgcg
    15241 attccttcct gcacgtacag cagaaatcaa tgaatcgcat aaaaatacaa aaaaagtata
    15301 gatgttcagc aagaattggc gggtaaattg ctatttaagt caaaaac
//
LOCUS       DAOFMX010000090        15044 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.090, whole genome shotgun sequence.
ACCESSION   DAOFMX010000090 DAOFMX010000000
VERSION     DAOFMX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.090"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..234
                     /locus_tag="QBA20_003346"
     CDS             <1..234
                     /locus_tag="QBA20_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDQ6293769.1"
                     /translation="LGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPA
                     LSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            244..882
                     /locus_tag="QBA20_003347"
     CDS             244..882
                     /locus_tag="QBA20_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDQ6293770.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            885..2048
                     /locus_tag="QBA20_003348"
     CDS             885..2048
                     /locus_tag="QBA20_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDQ6293771.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2132..3757
                     /locus_tag="QBA20_003349"
     CDS             2132..3757
                     /locus_tag="QBA20_003349"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDQ6293772.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3874..4149)
                     /gene="yjfN"
                     /locus_tag="QBA20_003350"
     CDS             complement(3874..4149)
                     /gene="yjfN"
                     /locus_tag="QBA20_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDQ6293773.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4298..4627)
                     /gene="bsmA"
                     /locus_tag="QBA20_003351"
     CDS             complement(4298..4627)
                     /gene="bsmA"
                     /locus_tag="QBA20_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDQ6293774.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4809..5558
                     /gene="yjfP"
                     /locus_tag="QBA20_003352"
     CDS             4809..5558
                     /gene="yjfP"
                     /locus_tag="QBA20_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDQ6293775.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5555..6310)
                     /gene="ulaR"
                     /locus_tag="QBA20_003353"
     CDS             complement(5555..6310)
                     /gene="ulaR"
                     /locus_tag="QBA20_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDQ6293776.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6418..7482)
                     /gene="ulaG"
                     /locus_tag="QBA20_003354"
     CDS             complement(6418..7482)
                     /gene="ulaG"
                     /locus_tag="QBA20_003354"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDQ6293777.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7837..9234
                     /gene="ulaA"
                     /locus_tag="QBA20_003355"
     CDS             7837..9234
                     /gene="ulaA"
                     /locus_tag="QBA20_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDQ6293778.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9250..9555
                     /gene="ulaB"
                     /locus_tag="QBA20_003356"
     CDS             9250..9555
                     /gene="ulaB"
                     /locus_tag="QBA20_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDQ6293779.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9565..10029
                     /gene="ulaC"
                     /locus_tag="QBA20_003357"
     CDS             9565..10029
                     /gene="ulaC"
                     /locus_tag="QBA20_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDQ6293780.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10043..10693
                     /gene="ulaD"
                     /locus_tag="QBA20_003358"
     CDS             10043..10693
                     /gene="ulaD"
                     /locus_tag="QBA20_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDQ6293781.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10703..11557
                     /gene="ulaE"
                     /locus_tag="QBA20_003359"
     CDS             10703..11557
                     /gene="ulaE"
                     /locus_tag="QBA20_003359"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDQ6293782.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            11557..12243
                     /gene="ulaF"
                     /locus_tag="QBA20_003360"
     CDS             11557..12243
                     /gene="ulaF"
                     /locus_tag="QBA20_003360"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDQ6293783.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12372..12647)
                     /locus_tag="QBA20_003361"
     CDS             complement(12372..12647)
                     /locus_tag="QBA20_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDQ6293784.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12974..13369
                     /gene="rpsF"
                     /locus_tag="QBA20_003362"
     CDS             12974..13369
                     /gene="rpsF"
                     /locus_tag="QBA20_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDQ6293785.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13376..13690
                     /gene="priB"
                     /locus_tag="QBA20_003363"
     CDS             13376..13690
                     /gene="priB"
                     /locus_tag="QBA20_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDQ6293786.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13695..13922
                     /gene="rpsR"
                     /locus_tag="QBA20_003364"
     CDS             13695..13922
                     /gene="rpsR"
                     /locus_tag="QBA20_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDQ6293787.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13964..14413
                     /gene="rplI"
                     /locus_tag="QBA20_003365"
     CDS             13964..14413
                     /gene="rplI"
                     /locus_tag="QBA20_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDQ6293788.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14484..>15044)
                     /locus_tag="QBA20_003366"
     CDS             complement(14484..>15044)
                     /locus_tag="QBA20_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDQ6293789.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3513 a         3667 c         4059 g         3805 t
ORIGIN      
        1 ttaggttacg ttatcccatt atccagtgcg gcaatcaatg cggtgagtat tccagactat
       61 ttcgcctggg gcgggattgc actggtgatt cagttactcg tttttgctgg tgtcagactt
      121 tatatgcccg cattaagcga aaaaattatt aatcacaata ccgcagcagg aatgtttatg
      181 ggaaccgccg cgctggctgg cggtattttt aacgcagctt gtatgacatg gtaatggacg
      241 atcatggcca gaaaacgcaa atcaagaaat aacagtaaaa ttggtcacgg agcgatcagt
      301 cgcattggta gaccgaataa tccttttgaa ccgcgtcgca atcgctacgc acaaaaatac
      361 ttaacgttgg cgctaatggg cggtgccgct tttttcgtat tgaaaggttg tagcgatagt
      421 agcgatgtcg ataacgacgg cgacggaaca ttttacgcga cagtgcagga ttgtattgat
      481 gacggtaata atgccgacat ttgcgcgcgt ggctggaaca acgccaaagc ggcattttat
      541 gccgatgttc caaagaatat gactcagcag aactgtcagt ctaagtacga aaattgctac
      601 tacgacaatg ttgaacagag ttggatcccg gtcgtttctg gatttttgtt aagccgggtt
      661 attcgtaaag atcgcgatga gccgttcgtc tataacagcg gcggatcctc atttgcttcc
      721 cgccccgtct ggcgcaacac ttccggtgat tactcctggc gcttcggttc tggcaaaaaa
      781 gagtcttact cttcaggcgg ctttaccacc agaaaagcgt cgaccgtttc gcgcggcggc
      841 tatggtcgtt cttccagcgc ccgtgggcat tggggaggct aatcatgctg agacacaacg
      901 ttcctgtgcg gcgggatctg gaccagatcg ccgctaacaa cggtttcgac tttcatatca
      961 tcgacaatga aatctattgg gacgagagtc gggcttaccg ttttactctg cgccagattg
     1021 aagagcagat cgaaaaaccg actgcggaac tgcatcagat gtgccttgag gtggtggatc
     1081 gcgcggttaa agacgaagag atcctgacgc aactggcgat cccgccgttg tactgggatg
     1141 tgatcgccga aagctggcgc gcacgcgatc cttcgctgta tggccggatg gattttgcct
     1201 ggtgtggcaa tgcgccggtg aagctgctgg agtacaacgc cgatacgccg acctcgttgt
     1261 atgagtcggc ttatttccag tggttgtggc tggaggatgc ccggcgcagc ggcattattc
     1321 cgcgtgatgc cgatcagtac aacgccattc aggaacgcct gatttcgcgc tttagtgagc
     1381 tttacagtcg ggaaccgttt tatttttgct gctgtcagga caccgatgaa gacaggagta
     1441 ccgtgctgta cttgcaggac tgcgcccagc aggcagggca ggagtcgcgg tttatctaca
     1501 ttgaagatct cggtttgggc gtcggcggcg tactgaccga tcttgatgat aatgtcatcc
     1561 agcgtgcatt taagctgtat ccgctggagt ggatgatgcg tgacgataac ggtccgctgc
     1621 tgcgcaagcg tcgcgagcaa tgggtggagc cgttatggaa aagtatcttg agtaataaag
     1681 ggctaatgcc gctgctttgg cgcttcttcc ctggtcatcc taaccttctt gcatcctggt
     1741 ttgagggtga aaaatcgcag attgccgctg gcgaaagcta tgtgcgtaaa ccgatctact
     1801 cgcgcgaagg cggaaacgtc accattttcg acggtcagaa taacgtcgtt gaccacgctg
     1861 atggtgatta cgccgatgaa ccgatgatct accaggcgtt tcaacctctg ccgcgatttg
     1921 gcgatagcta cacactcatc ggtagctgga ttgtcgatga tgaagcgtgc ggaatgggga
     1981 tccgtgaaga taacacactg atcaccaaag acacctcacg tttcgttccg cattacattg
     2041 ctggataaga atgttttagc aatctctttc tgtcatgaat ccatggcagt gaccatacta
     2101 atggtgactg ccattgatgg agggagacac agtgcactgg caaactcaca ccatttttaa
     2161 tcaacctata ccattaaata acagcaactt atacctgtct gatggcgcgc tttgcgaagc
     2221 ggtaacgcgt gaaggtgctg gctgggatag cgattttcta gccagtattg gtcagcagtt
     2281 aggaacggct gaatcccttg aactggggcg gctggcgaat gtaaacccgc ctgaactgtt
     2341 gcgctacgat gcccaggggc ggcgtctgga cgatgttcgt ttccatcccg catggcacct
     2401 gctgatgcag gcgttatgta ccaatcgggt gcacaatctt gcctgggaag aagacgctcg
     2461 ctccggcgca tttgtggcgc gcgcggcgcg ttttatgtta catgcgcagg ttgaggctgg
     2521 gtcgttatgt ccaataacga tgacctttgc cgccacgcca ttgttgttac agatgttacc
     2581 cacgccgttt caggactgga ccacgccgct gctgagcgat cgctacgatt ctcacttatt
     2641 gccaggtggg caaaaacgcg gtttgttgat tggcatggga atgacggaaa agcagggcgg
     2701 ttccgatgtc atgagcaaca ccacccgtgc agagcgtttg gaagatggct cttatcggct
     2761 ggtggggcat aaatggtttt tctcggtgcc gcaaagcgat gcgcatctgg tgctggcgca
     2821 gaccgcgggc ggtctgtcct gcttttttgt gccgcgcttt ttgcctgacg ggcaacgcaa
     2881 cgcgattcgc ctcgagcggc tgaaagataa gctgggtaat cgctctaacg ccagttgcga
     2941 agtggagttt caggatgcca ttggctggtt attggggcag gaaggggaag gaattcgtct
     3001 gatcctgaaa atggggggga tgacgcgttt tgattgcgcc ctgggtagcc atgccatgat
     3061 gcgccgtgca ttttcgctgg cgatttatca tgcacatcaa cgccatgttt ttggtaatcc
     3121 attgatccaa cagcccctta tgcgtcatgt cttaagtcgc atggcacttc agcttgaagg
     3181 gcaaacggcg ttgctgtttc gtcttgcgcg agcgtgggac cggcgtgccg atgccaaaga
     3241 agccctgtgg gcgcgtttat ttacgcctgc ggcaaaattt gtgatctgca aacgaggtat
     3301 gccgtttgtg gccgaagcga tggaggtgct gggcggcatt ggttattgcg aggagagcga
     3361 gctgccgcgg ctttaccggg agatgccggt aaacagtatt tgggaaggtt ccggcaatat
     3421 tatgtgcctg gatgtgttgc gcgttctcaa taagcaagcg ggcgtatacg acttattgtc
     3481 ggaagcattt gtggaagtga aagggcagga tcgctatttt gatcgcgcgg ttcgtcgttt
     3541 acagcagcag ctgcgtaagc cagctgaaga actggggcga gagattactc atcagctatt
     3601 cctgctgggc tgcggtgcgc aaatgttgaa atatgcttct ccgccaatgg cgcaggcgtg
     3661 gtgtcaggtg atgttagata cgcgcggcgg cgtacggttg tcagagcaga tccagaatga
     3721 tttattgctg cgggcgacgg ggggagtgtg tgtgtaagcg tatacgactg atgcgacgct
     3781 ggtttcgatt aactaaatga aatatgtgaa aattgtaggc cggacaaggc gctcgcgccg
     3841 catccggcat tgttcatttg ctgtagatga cactcatgca tacagtatcg cctgtacgcg
     3901 ccagttatca ggctgctggt cttcgtacat ggtaataatg cggtaccatg acgcaccttg
     3961 ttcatcggcc tttaacgcca cgatacgttc cacgtcctgc ggatcccctg aaatattgct
     4021 aactgagatc tgacctattt cattcaggcc cgtcacgcag tcagcactgg cgaattctgc
     4081 agactgtgcc gtggcgctcg caggataagt cactaattgc agcgagggtg aggcaagtaa
     4141 ttgtttcatc gtcagctcca tttttcgttt ccccacatgt tgcagggcat tccatcgcgg
     4201 taataaacgc ccaagcttcc agccggtaat ttattgtgca caggtaaacg catagggacg
     4261 caaagcaatg taaagctgac tatgtaagtt gtctgattta tttacggtac aaaatggctt
     4321 gtgaatacca ctgtcctgtc acgatggttt cgtccaccat gacgacaacg taataatcag
     4381 cttttgcagc gacagctttt gctctgattt ctgctaatgc gtcatccgga gaaccccgaa
     4441 ccatcgtgct tacgctacct attcgctgta acccttgcgt ctggtcgcgg cgaatctctt
     4501 gcggatggtc cgttactggc ggtgctggct gtggcgtacc ttgcagtgcg ctacaggcac
     4561 ttaacatcaa caccaataat aaactggcaa accggtaaat aacgctatta cgtttcctgc
     4621 taaccatagt gtagtgcctt attaatttaa ctttggggga atgttcccga attgttacct
     4681 ggtacgcgat tttcgaatga aaacgtgacg aaagcgtaac ccacatctca acattatgaa
     4741 ctaataccgc cagcagaaac ttgtgctaag gatttcttca tgtcatacca gacaaaaagg
     4801 agagacagat gattgaaata gaatcacgcg agctggcaga tattcccgtt cttcatgctt
     4861 atcctgtcgg gcaaaaagat accccgttac cgtgcgtaat tttttatcac ggctttactt
     4921 catccagcct ggtgtatagc tattttgccg ttgcgctggc gcaggctggt ttgcgggtga
     4981 tcatgccgga tgcgcccgat cacggtagcc gttttagtgg cgacgaagcg cggcggttaa
     5041 atcaattctg gcaaatcttg ctacaaagta tgcaggaatt cactacttta cgtgcggcaa
     5101 tagccgaaga aaactggctg cttgatgacc gtctggcagt cggtggcgcg tcgatgggcg
     5161 cgatgacggc actgggaatt accgctcgcc accccacggt gaaatgtacc gccagcatga
     5221 tgggatcggg ctattttaca tcgctcgccc gttcactgtt tccaccgctg atacctgaaa
     5281 cggcagcaca gcagaatgaa ttcaataaaa ttgtcgcgcc gctggcagag tgggaagcga
     5341 caaaccacct ggaacaactt ggggacagac ctctactgct gtggcatggc ctcgacgatg
     5401 acgttgtgcc tgccgacgaa tcactacgtt tgcagcaggc cttaagcgag acgggacggg
     5461 ataaactgct aacctgttca tggcagccag gcgtgcgtca ccgtattacg cctgaggcgt
     5521 tagatgctgc cgtgacattt ttccgccagc atctttaaac acgcagaatg ctgacccctt
     5581 gcgcttccag ttgttgcagg atttccgggt tagcattttt gccggtgatc agcatatcga
     5641 tttgatcggc acggctaaaa agcattcccg cgcgttcgcc aatcttactg ctatcaacca
     5701 gtaccaccag tttccctacc acgctcagca tcttctgctc tgccattgct gtcagcatat
     5761 cggttttata caacccttct gcggtcagcc cttttccgct ggtaaacatc cagtgcccgg
     5821 catagagact gttttcgctg ccctgcgggc ttaaagtgat ggactgactt ttgttgtact
     5881 gtccacccat aatgatcacg ctgtcatgtt cttgatcgat caggtaattt gccagcggta
     5941 gataattagt gatgatttgc actggcttgc cacacatttc ccgcccaagc agaaacgcgg
     6001 tggagccgca gttgatgact acgctttcgc ccggattaac cagctgcgag gccgctttag
     6061 caatacgtac tttttcatcg tgattctgcg cctgatgcag attcatcggc gtccagcgcg
     6121 ggcgctgttg ggtaatagct tctgcgccat tgcgcacctt tttcagtttg ccgctttcgc
     6181 caagtttatt gatatcgcgt cgcgcagtgg caggcgaaat tcccagacgc tcaacgactt
     6241 tctcaacggt cacaaagccc aattgtgcga gcatttccag gaggatttga tgtctttgtg
     6301 cttcagtcat gagctattcc gataaaattt gattttttta gatgatattt gaaatagcca
     6361 ggaaatacta cgccggatag cgcgaacttc tccacgctat ccggtataaa cgacagatta
     6421 caggaatgac ttgaacggca gatccggttc aatagtgaag caatcatcga aaccgcgcgg
     6481 atagtggtac tcgaagttgt ctttatccag cggccaggta aatttgccgc caacctgcca
     6541 gataaacggc ttgaagccat acttcagacg atcttttttg atctcccaca gcacgcggat
     6601 ctcttgcgga tcggcctgga agtttgacca gatgtcgtgg tggaacggga taaccacttt
     6661 cgcattcagg gcttcaccca tacgcagcat atcggcgctg gtcattttgt cggtaatacc
     6721 gcgcgggttc tcaccatacg atcctaacgc cacatcgatc tgatgttcgt taccgtgctt
     6781 cgcataatag ttagagtagt gggaatcgcc actgtgatat agggaaccgc caggagtttt
     6841 gaacaggtag tttactgcgc gatcgtccat gccatctggc agtacgccag ccgctttttg
     6901 atcggcaggc agggtgatca gtgcagtacg gtcgaaagcg tcaagcgcat gaatttcaat
     6961 gtctttcact tttacaacat cgcccggttt gaccacgatg caacgctctt tcggtacgcc
     7021 ccagccaatc cacaaatcaa cgcaggtttt cggtccaatg aatggtacgt catcagcaca
     7081 gttctgcatc acggcagcag cgacgttaac gtcgatatga tcgttgtgat cgtgagtcgc
     7141 cagtaccgcg tcgatctggc gaatcgcaaa cggatcaaga acaaacgggg tggtgcgtag
     7201 gtttggctgc agttttttca cgccagccat gcgctgcatc tggtgaccct gtttcattaa
     7261 cgggttaccg tgactttgtt tgccagtgcc gcaccagaaa tcaacgcaaa cgttggtgcc
     7321 accttctgat ttcaaccaga tcccggtgca gcctagccac cacatcgcaa atgtgccagg
     7381 ggcgacctgc tcttgttcaa tttcttcatt cagccagcta ccccactccg ggaaagtgct
     7441 caggatccag gattctcggg tgatactttt cactttactc atcgccattt accttcatga
     7501 tagttcaatt cgaatcaaca tgtgattggt tttgattaat cctgacacta ttccttcagg
     7561 aaggcaatga ccattttttg actttttgcc agggaagttg ttgttgattt tgagtgtgga
     7621 aagatttaat ggaatgtgta gttaaattaa ctaaatgaat ttaaataaat aattgtttcg
     7681 ttgtgtgaat cacactctat ccgtgtggaa ttatttgcgg gtcgcgtcac atttaatcat
     7741 aaataatctt gttgtgatta cttttgaaaa ttagagtgag tgcacaacat tccgggtgtg
     7801 cggaataccc ggttacctct tcttcaggag atcgttatgg agatcctcta caacatcttt
     7861 accgtgtttt ttaaccaggt catgaccaat gccccgttgt tgctgggtat tgtgacctgt
     7921 ctgggctaca tcctactgcg caaaagtgtc agcgttatta ttaaaggcac gattaaaacc
     7981 ataattggtt tcatgttgtt gcaggcaggg tccggcatcc tcaccagcac cttcaaaccg
     8041 gtggtggcga aaatgtccga agtctacggc attaacggcg caatttccga tacttacgcg
     8101 tcgatgatgg caaccatcga ccgcatgggc gatgcctata gctgggtagg ttacgcggtg
     8161 ttgttagcgc tggcgctgaa catctgttac gtgctgctgc gtcgcattac cggtattcgt
     8221 accatcatgc tgactggtca tatcatgttc cagcaggcgg ggctgattgc cgttacgttg
     8281 tttattttcg gctactccat gtggacaacc attatctgca cggcgattct ggtttcgctc
     8341 tactggggca ttacctccaa catgatgtac aagccgactc aggaagtgac ggacggctgc
     8401 ggtttctcca tcggtcacca gcaacagttt gcatcattaa ttgcctataa agttgcgccg
     8461 ttcctcggca aaaaagagga aagcgttgaa gacctcaaac tgccaggctg gctgaatatt
     8521 ttccacgaca acatcgtctc cacggcgatt gtcatgacca tcttctttgg tgccattctg
     8581 ctctccttcg gtatcgacac cgtgcaggcg atggcaggca aagtgcactg gacggtctat
     8641 atcctgcaaa ccggtttttc cttcgcggtg gcgatcttca tcatcactca gggtgtgcgc
     8701 atgtttgtgg cggaactctc tgaagcgttt aacggtatct cccagcgcct gatccctggt
     8761 gcggttctgg cgattgactg tgcggctatt tatagcttcg ctccgaacgc cgtggtctgg
     8821 ggctttatgt ggggcaccat cggtcagctg attgcggttg gcatcctggt cgcctgcggt
     8881 tcctcgatcc tgattatccc aggctttatc ccgatgttct tctccaacgc caccatcggc
     8941 gtgttcgcta accacttcgg cggctggcgt gcggcgctga agatttgtct ggtgatgggg
     9001 atgatcgaaa tctttggttg cgtctgggtg gtgaaactta ccggtatgag tgcctggatg
     9061 gggatggcgg actggtcgat tctggcaccg ccgatgatgc agggcttctt ctccatcggt
     9121 atcgccttta tggccgtcat cattgtaatt gcactggctt atatgttctt cgctggccgc
     9181 gcactgcgcg cagaagaaga tgcagaaaaa caattggcag aacagtctgc ttaataagga
     9241 gttttgatta tgaccgtacg tattctggct gtgtgtggca acggacaagg cagttccatg
     9301 atcatgaaga tgaaagtgga ccagttttta acccaatcaa acattgacca cacggtaaat
     9361 agctgcgcgg ttggcgaata caaaagcgag ttgagcggcg cggatatcat catcgcttct
     9421 acgcacattg cgggcgaaat caccgtgacc ggcaacaaat acgtggttgg cgtgcgcaac
     9481 atgctctctc ctgccgactt tggcccgaaa ctgctggaag tgatcaaagc gcatttcccg
     9541 caggatgtga agtaaggacg cgccatgaaa ttacatgatt cgctggcgga gaataaatcc
     9601 atccgcctgc aggctgaagc tgagacatgg caggacgcag taaaaatcgg cgttgacctg
     9661 ctggtggcgg cggatgttgt cgagccgcgt tactaccagg cgattctgga tgcggttgaa
     9721 cagcacggtc cgtactttgt gctcgcacca ggcctggcaa tgccgcacgg tcgcccggaa
     9781 gagggcgtta agaaaaccgg ttttgcgctg gtgacgctga aaaagccgct ggagttcaac
     9841 cacgaagata acgacccggt ggatatcctc atcaccatgg cggcggtcga tgccaacacg
     9901 caccaggaag tgggcatcat gcagatcgtc aacctgtttg aagatgaaga aaatttcgac
     9961 cgtttacgtg cctgccgtac cgagcaggaa gtactggatc tcattgaccg caccaacgcg
    10021 gcagcttaag aaggaattga agatgtcatt accgatgttg caagtcgcgc tggacaacca
    10081 gactatggat agcgcctacg aaaccactcg cctgattgcc gaagaagtcg acattatcga
    10141 agtgggtacc attctgtgcg tgggcgaagg cgtgcgtgcg gttcgcgatc tgaaagcgct
    10201 ctacccgcac aaaatcgtgc tggcagacgc caaaattgcc gatgcgggca aaattctttc
    10261 tcgtatgtgc ttcgaagcca acgctgactg ggtgaccgtg atttgttgtg cggatatcaa
    10321 caccgccaaa ggtgcgctgg acgtagcaaa agagtttaac ggcgacgtgc agatcgaact
    10381 gaccggttac tggacctggg aacaggcgca acagtggcgc gacgcaggta ttcagcaggt
    10441 ggtttatcac cgcagccgtg acgcgcaggc cgctggcgtg gcgtggggtg aagccgatat
    10501 cactgcgatc aaacgtcttt ccgatatggg cttcaaagtc accgtcaccg gtggcctggc
    10561 gctggaagat ctgccgctgt tcaaaggcat tccaattcat gtgtttatcg ccggtcgcag
    10621 catccgtgat gccgcttctc cggttgaagc cgcgcgtcag ttcaaacgtt ccatcgccga
    10681 actgtggggc taaggagcgg atatgttgtc caaacaaatc ccgcttggca tctatgaaaa
    10741 agcgctcccc gccggggagt gctggctgga acgcctgcga ctggcaaaaa cgttgggctt
    10801 cgattttgtc gagatgtcgg tagatgaaac taacgagcgc ctgtcgcgcc tcgactggag
    10861 ccgcgaacag cgtctggcgc tggtgaatgc tattgttgaa accggcgtgc gtgtgccgtc
    10921 catgtgcctt tctgcccatc gtcgtttccc gttgggcagc gaagatgacg cggtgcgggc
    10981 gcaggggctg gagattatgc gtaaagccat ccagttcgcc caggacgttg gcattcgcgt
    11041 gatccagctg gcgggctatg acgtttacta tcaggaagcc aataacgaaa cgcgtcgtcg
    11101 tttccgtgac ggcctgaaag agagcgttga gatggcgagc cgcgcgcagg tgacgctggc
    11161 gatggagatc atggattatc cgttgatgaa ctccatcagc aaggcgctgg gttacgcgca
    11221 ctatctcaac aacccgtggt tccagctcta cccggatatc ggcaacctgt cggcgtggga
    11281 caacgatgtg cagatggagt tgcaggccgg aatcgggcat atcgtcgcgg tgcatgtgaa
    11341 agacaccaaa ccgggcgtct tcaaaaacgt gccatttggc gaaggtgtgg tggatttcga
    11401 acgttgcttc gaaacgctca aacagagtgg ctattgcggg ccgtacctga ttgagatgtg
    11461 gagcgaaacg gcggaagacc cggcggcaga agtggtgaaa gcgcgtgatt gggtgaaagc
    11521 gcgcatggcc aaagcaggca tggtggaggc ggcataatgc aaaagctaaa acagcaggtg
    11581 tttgaagcca acatggatct gccgcgctat gggctggtga cctttacttg gggcaacgtc
    11641 agcgctatcg accgcgaacg cgggctggtg gtgatcaagc ccagcggcgt tgcctacgaa
    11701 accatgaaag cagacgatat ggtggtggtc gatatgagcg gcaaggtggt ggaaggggag
    11761 tatcgcccat cttccgacac tgcgacgcat ctcgaactct accgtcgtta cccgtcgctt
    11821 ggcggcattg tccataccca ctccactcat gccaccgcat gggcgcaggc ggggctggcg
    11881 atcccggcat taggcaccac gcacgctgac tacttctttg gtgatatccc gtgtacgcgt
    11941 ggattaagcg aagaagaggt gcagggtgag tatgaactga acactggcaa agtgattatc
    12001 gaaacgctgg gcaacgccga gccgctgcat acgccgggaa ttgtggtgta tcagcacgga
    12061 ccgttcgcct gggggaaaga tgcccacgac gcggtgcata acgcggtagt gatggaagaa
    12121 gtggcgaaaa tggcgtggat tgcgcgcagt attaatccac aactcaatca tatcgacagc
    12181 tacctgatga ataaacactt catgcgtaag cacggtccta acgcttatta cgggcagaag
    12241 taaaacacgc gctgcggaaa tttccttcct cgggagataa ctggtctaat tccgcagccg
    12301 tttttcaaaa aaagccccct gcgaaggggg caaagcaaac tatggcaatg tttcgttggt
    12361 tatacctggt gctagcgata aatatccgcg ctggcgtgca tattgccgtt actccccggt
    12421 tcccgcatca gaattacgtg gtagtacgtt gcgccttgcg catctgtttc ttcatttaat
    12481 gcctgacgtg cttcgctttc agtggcgaaa ttatgattga tataaatcac gcctaagctt
    12541 tgcacatcgt ccatatttct ggcctggtgg ttattaattt caatggctgc ccatgtattt
    12601 gcacttagca aaagcacagc cagaagggct aaaactcgac tgaacataga tacctcctcg
    12661 acggctgact ttgtgtgctc tcctctgtga tgatcttctg atttaatttt aatcaatgat
    12721 aaagaagttg atggtgacca tttctgatgc agttgttcaa aaaaacactg tgacggaatg
    12781 tgatgaactt caaatcagcg gtttagaggt tatttgcgaa aggggagatt tatttctgct
    12841 ccgcccttga gtttagcggg gtatgccagt actataacgc gtcatttttc agccgacctt
    12901 taacacgttc cttgcctccc cgggattcgg ctgacccaga caggaggctg aataatccgt
    12961 aaggagcaat tcgatgcgtc attacgaaat cgtttttatg gtccatcctg accagagcga
    13021 acaggttccg ggcatgatcg agcgctacac tgctgccatc actggtgcag aaggcaagat
    13081 ccaccgtctg gaagactggg gccgccgtca gctggcttac ccgatcaaca aactgcacaa
    13141 agcacactac gttctgatga acgttgaagc tccgcaggaa gtgatcgatg agctggaaac
    13201 taccttccgc ttcaacgatg ccgttatccg cagcatggtt atgcgtacca agcacgctgt
    13261 taccgaagca tctccgatgg ttaaagcgaa agacgagcgc cgtgagcgtc gcgatgattt
    13321 cgcaaacgaa accgctgatg atgctgaagc tggggattct gaagagtaat ttctgatgac
    13381 caaccgtctg gtgttgtccg gcaccgtgtg caggactccc cttcgaaagg tcagtccatc
    13441 aggaattcct cactgccagt tcgtgcttga gcatcgttct gtgcaggagg aagccggctt
    13501 tcaccggcag gcatggtgtc aaatgcccgt tattgttagc ggacacgaaa accaggccat
    13561 tactcacagt ataacggtcg gcagtcgcat aaccgttcag gggttcattt catgccacaa
    13621 ggcaaagaac ggactgagca aaatggtttt gcatgccgag cagattgaat tgatagattc
    13681 tggagactag ccatatggca cgttatttcc gtcgtcgcaa gttctgccgt ttcaccgcgg
    13741 aaggcgttca agagatcgac tataaagata tcgctacgct gaaaaactac atcaccgaaa
    13801 gcggtaagat tgtcccaagc cgtatcaccg gtacccgtgc aaaataccag cgtcagctgg
    13861 ctcgcgctat caaacgcgct cgctacctgt ccctgctgcc gtacactgat cgccatcagt
    13921 aatcggtcac ggtccattaa tacgactttg agaggataag gtaatgcaag ttattctgct
    13981 tgataaagta gcaaacctgg gtagcctggg tgatcaggta aacgttaaag cgggctatgc
    14041 tcgtaacttc ctggtaccgc agggtaaagc tgttccagct accaagaaaa acattgaatt
    14101 cttcgaagca cgtcgcgctg aactggaagc taaactggct gaagttctgg cagctgctaa
    14161 tgctcgcgct gagaaaatca atgcactgga aactgttacc atcgcgtcta aagctggcga
    14221 cgaaggtaaa ctgttcggtt ccatcggtac tcgcgacatc gctgacgctg taactgcagc
    14281 tggcgttgaa gtggctaaga gcgaagttcg tctgccgaac ggcgttctgc gtaccactgg
    14341 cgaacacgaa gtgagcttcc aggttcacag cgaagtattc gcgaaagtga tcgtaaacgt
    14401 agtagctgaa taattcgtta ttcaacgaga cgtaaaaacg ccgaccattg gtcggcgttt
    14461 tgctttctat ttttcgtcag gcattaattt cgcaagtaga tccattcacc attctcttgt
    14521 gtccataagt caggatggtt tgctggcgtt agatgcatac gatcactcct tatgtctcgg
    14581 tcctgcggca ctcgctcgaa acctaaaccc gagtagattg cggctgaatt tgataaggaa
    14641 cgtagaccaa ttttcaaatc atggggtaaa tcagaatacg cgtttagcaa tgcagcaatg
    14701 atgaatccga gggcattaga aacgcctgta acagtgtgaa gttggtcgat ataaagatcg
    14761 attgttggta gttcagatga ccagcaatta ggcatttggg caggaagggt tacaacgtat
    14821 cctatcgttt ggccggtata gtctggagta ctataaccag gtcccaaaac aacctcgtgt
    14881 attgtgtatt tactgttggg ttcgaaggat tcgacatctt gcaataaata tttcagttct
    14941 gcggttaacc tcgcacccgc gtaagcttta ttgtatttct gggcgtgaat attatctaat
    15001 gtagcttcat ggttccatcc aaaagaatta tcaccattca ttgt
//
LOCUS       DAOFMX010000091        14974 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.091, whole genome shotgun sequence.
ACCESSION   DAOFMX010000091 DAOFMX010000000
VERSION     DAOFMX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.091"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..176)
                     /locus_tag="QBA20_003367"
     CDS             complement(<1..176)
                     /locus_tag="QBA20_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293790.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNELKHG"
     gene            complement(592..1533)
                     /gene="fabY"
                     /locus_tag="QBA20_003368"
                     /gene_synonym="yiiD"
     CDS             complement(592..1533)
                     /gene="fabY"
                     /locus_tag="QBA20_003368"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDQ6293791.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="QBA20_003369"
     CDS             complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="QBA20_003369"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDQ6293792.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2012..2884)
                     /locus_tag="QBA20_003370"
     CDS             complement(2012..2884)
                     /locus_tag="QBA20_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDQ6293793.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="QBA20_003371"
     CDS             complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="QBA20_003371"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDQ6293794.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3576..4361)
                     /locus_tag="QBA20_003372"
     CDS             complement(3576..4361)
                     /locus_tag="QBA20_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ6293795.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="QBA20_003373"
     CDS             complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="QBA20_003373"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HDQ6293796.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5459..6279
                     /locus_tag="QBA20_003374"
                     /pseudo
     CDS             5459..6279
                     /locus_tag="QBA20_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            6303..7181
                     /gene="yihT"
                     /locus_tag="QBA20_003375"
     CDS             6303..7181
                     /gene="yihT"
                     /locus_tag="QBA20_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HDQ6293797.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7198..8439
                     /gene="yihS"
                     /locus_tag="QBA20_003376"
     CDS             7198..8439
                     /gene="yihS"
                     /locus_tag="QBA20_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HDQ6293798.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8577..9479
                     /locus_tag="QBA20_003377"
     CDS             8577..9479
                     /locus_tag="QBA20_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HDQ6293799.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9678..11714
                     /gene="yihQ"
                     /locus_tag="QBA20_003378"
     CDS             9678..11714
                     /gene="yihQ"
                     /locus_tag="QBA20_003378"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HDQ6293800.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11760..13134
                     /locus_tag="QBA20_003379"
                     /pseudo
     CDS             11760..13134
                     /locus_tag="QBA20_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13177..14580
                     /locus_tag="QBA20_003380"
     CDS             13177..14580
                     /locus_tag="QBA20_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6293801.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14648..>14974
                     /gene="ompL"
                     /locus_tag="QBA20_003381"
     CDS             14648..>14974
                     /gene="ompL"
                     /locus_tag="QBA20_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HDQ6293802.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3677 a         3859 c         3725 g         3713 t
ORIGIN      
        1 aaaccatgct tcagttcgtt aacacaatcg cacagttgct cctcaccttc cattttaagg
       61 caaagtgaaa attgatttaa tagcgtttgc ttcggtgatt tattactcat tagttgtttt
      121 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
      181 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
      241 gtaaatgaat gatactgaca atgtatgcca gcccattatc tttgcataat atttgtgagc
      301 tatagatttt aaaaatattt atgaaaaaag tatggcacca gagggggttt tgactatcaa
      361 aaatcgctca aacctgaact aatatgatga gttaagcatt atctgcaata attgccactc
      421 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggtgc
      481 caacgaagaa taaaaattta taaagttctt attcaatata accggaatag tcagggactg
      541 atgtaaatga ccggaatgaa tgagagaggc agcaccgccc ctctccataa gctactcttc
      601 ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg ggcagaacga tatacgtgcc
      661 ttcaaacact gcacccggcg tctcgtcgcc aaagatttcg acctgcatct gcacccgtgc
      721 ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag gcaccaaggt cggctaccgc
      781 atggggttta ccgctaatcg gtttgctgta gcggatatgc gcatccgcca gaataatcgt
      841 tccgccgagg tggcgttcac gcagcatcag ccagataagc ccccaaccgg tgagcgtcgc
      901 cagcgagaat aaactcccgg caaacagcgt atggtgcgga ttctgattgc cggtttccgg
      961 catggtggtg ataaattttt gcccggtata ttgttgaata cgcacgccca ttttttcact
     1021 aagcgggata tgttcgtacc acgcctgttg cagctgcgcg caccagtcgc cgcgatgcaa
     1081 aatatcatcc agagtggcga cgggcttaat catcaaaaaa tggcgaatcg gcgtggtggt
     1141 tggcgtggtg atttctccct gattaacaaa ccccagcttg gcgaaaaact ccaccgcgtc
     1201 ttcacgggcg ctacaggtca cgcgcttaac gccttcctga cgcgccaccg actccagggt
     1261 catcgccatc agcgttccta accctttgtc ctgcacgtcc gggtgaacga ccataaagcg
     1321 aatggacgct tcattgtcgg cattaatata caatcggcct accgccacca gattaccctg
     1381 ctcgtcgacg accatctggt gatgcgccat cgcatcccac gcgtcgcgtt ccgaaccttt
     1441 tggttgatgc aggggcttac gcaacatttc ccagcgaaac tgatagtaac gctctaactc
     1501 ttcttctgtt tgtggaacct gaaggtgata catagcgata ctctctcttg tttcccgtga
     1561 caaccctgga agctggctca tacctgcaac cagaatgtca cggggccatc attgaccagc
     1621 gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt tcatctcttg ctggcggcag
     1681 cgttcgacga aatagtcata taacgcctct gcgcgatccg gtgatgcgcc tttggagaaa
     1741 cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa actgggaaac caccagtaca
     1801 ctgccgcccg cctgttgcac gttgagattc atcttgcctt cggcatcgct aaagatgcgg
     1861 tagccgagca cacgctcgca cagacggttt gctttctgtt cgtcgtcatc cttttcgaca
     1921 cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca cttctccctc cacggtgacg
     1981 ctggcacggg ttacgcgttg aattaatgca atcatggttc gtcgtcttct tcttgttcag
     2041 ctgcttgttt tagtttgcgg tattccccga gagtgacagt aatttccgcg ccaagcaaga
     2101 cgatacacca tgtccagtag acccaaacaa agagaatggg gatcaccgcc agcacaccgt
     2161 aaatgagctg atatgacggg aacatggtga tataaagcgc gaaacctttc tttcctgctt
     2221 cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc gcggttaggt acgcggatgg
     2281 taggaacaat gctgtacagc aaccagaagg agatccacga caacagcaac ggaaaaatac
     2341 gcagcacgtt gtcgatgaca gtattgagat cgctcgccca gcgcagggag agcaaatagg
     2401 aactgatcgc cagactggcc cctgccagca gcggccccag cgttaaaatc atccagtaca
     2461 cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg ccagatggta ttcaacgcgc
     2521 tatcgatgga gtacatcaac aataacgcgg tgacgatcag cccgcacgcc ccaacggcgg
     2581 tcatcttgtt ggaattagca acaaattgtt cgatataccg ctgaataaca tcgccagtag
     2641 caggcagaaa gttggcaaaa ataaagtgac gtaactgaat gctgacgtcg gaaaacatgg
     2701 gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg cactaatgag agcaacgaca
     2761 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta
     2821 gttttagcca ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta
     2881 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt
     2941 aatgcctagc tgattagctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
     3001 gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg
     3061 catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc
     3121 ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc
     3181 ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc
     3241 ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc
     3301 ctctgcgaac gcttcgtcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat
     3361 atgaaaactc ttcttaagcg atgccagcgg aatacgcgtt aaatcgctcc aggctcccag
     3421 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
     3481 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
     3541 gcaaggtatg aagccaggtt aacggtcaca aaaatctacg cgtcttcctg gggaagaatg
     3601 atttcaattt ggctgtcttg taagcgtgat tttagcgcag cgggcggcag ctcgtcggta
     3661 aagagcgcag tgacctgtgc cacgttacca atttcaaccg ccgccgaagc atgatactta
     3721 gtgtgatcgg cgaccagcag aatatttctc gcgtgcgcca tcatcgtttt caccacgtta
     3781 gcttcgttta catcaaactc catcaacgcg ccatcgctct caatcgcccc aacgcttgtt
     3841 accagataat cagcacgaaa atcagccaca aaggacgccg ctgaagggcc aatgatcccg
     3901 ctattatgag agcgcaacgt accgccgggc accatcactt caaagcgcgg gttgtggtaa
     3961 agaatatgcg ccacacgcag gctgttggtg attatccgca aatgattatg gttaagtaac
     4021 gcccgggcaa catgctcaac agtcgtacca atggtgataa atattgttga accatcagga
     4081 atatagtctg ccaccgcttc ggcaatcgct ttttttttct cggtttgcga aacctcacgc
     4141 tgctcgaacg ccgtattaac gacgctggaa gcccgacccg cgccaccgtg atggcgcgta
     4201 atcaggcctt gctcgcttaa tttacgaata tcccggcgga ccgtctgcgt ggagacatcc
     4261 agcagatttg ccagctcatc aatattcata tacccacgct cggcgatcag catgaggagt
     4321 tggtcgtgcc gcgggttacc ggttagttcg gtaaggctca tgaaaattcc tcgaaaaacc
     4381 atcaccctgg cattttatac aaaaagtgac aaaaaagatc gggtttgatc acagtcaggg
     4441 atcccggctc gtccaccggg acgtgtgcat tttaacgccg ctacaccgct ggcgaagcgg
     4501 actgactccg ctaaatcccc acttgttgcc agcgccaccg ccaaagcgcc gtgaaaaaca
     4561 tcacccgcac ctgtggtatc taccacatca actttgaagg ccggttgatg ctgacgccca
     4621 ccattttcca gccagtcgca gcctgcgcta ccctgggtca catagacatg accatttgtg
     4681 agcgtttgtg cctgttttag cgcactggcc atctctttca cgcccgttaa gcgcgccaga
     4741 cccggttctg aaaaggccgc gtgatcgctt aatgccacca gctcactgat atcctgcggc
     4801 gtaatgtccc cgtccagaac ggtcatcaca cccgcctgac gggccagggt gaaggctttt
     4861 ttagcgccgt cgtgccagcg tacatctgcc agcacaacat cccactgaga gaaatcaatt
     4921 tcctccaacc actctgcgtc aggcagcaga tccgggctgg ggtagttaat gattatccgc
     4981 tcgcctttgg tatccaccat gatggcggat tgcgaagatt tcgcctggtt ataccgtttg
     5041 gtgtaacggg tgttaacgcc ccaggattcc agctctgcca gcaggctgtt gcccgcgtcg
     5101 tcatcaccta cgcgaccaat aaaatcgacc tgcgccccca gccttgccgc cgcaaccgct
     5161 gccgtcgccg ctggcccgcc accaacttcc gtataatttc tcgccacgta tttaccgctc
     5221 tccgtcggta acccttccac gtaatagatg cgatccatca cggttatacc tacacaagca
     5281 acacgaatca tggtcattcc ttaaacattt tgggatgcgt tcattttaat ttcaccaatt
     5341 gtttaaaaag tgacggctgt caattttttg acgataaatt acaaattcga tcaaaaacag
     5401 acaagcaaag catgtcataa atgacaaaaa gtgacatcac tgtattcagg agagggttat
     5461 ggcagcaatc gcgtttatcg gtttaggaca aatgggttcg ccaatggcga gcaatttatt
     5521 gcagcaaggg catcaacttc gcgtctttga tgtgaacgcc gaagctgtgc ggcatctggt
     5581 agacaaaggt gcgactcccg ccgccaaccc ggcgcaggcc gctaaagatg ccgaatttat
     5641 cattaccatg ttgccgaatg gcgatctggt gcgcaacgtg ttgttcggtg aaaacggcgt
     5701 ttgcgaaggc ttatctaccg atgcgctggt cattgatatg tccaccatcc atccgctgca
     5761 aatgccatta ccggtactct gttactgctg gctggcggca ccgctgaaca agttgaacgt
     5821 gccacaccga tcctgatggc gatgggcagt gagttgatca acgctggcgg tccgggcatg
     5881 gggatccgcg ttaagctcat caacaactac atgagcatcg cgctcaatgc gctttcggca
     5941 gaagccgccg ttttgtgcga agccctgaat cttcccttcg atgttgccgt caaagtgatg
     6001 agcggtaccg ccgccggtaa aggtcacttc accacttcct ggccaaacaa agtcctcagc
     6061 ggagatcttt ctcccgcctt catgatcgat cttgcccata aggatcttgg catcgccctt
     6121 gatgtcgcca accagctgca tgtgccaatg ccgctggggg ccgcctcacg ggaggtttat
     6181 agccaggcgc gcgcagcggg tcacggtcgc caggactggt ccgccattct ggaacaggtc
     6241 cgtgtcagtg ccgggatgac tgccaaagta aaaatgtaac gactggataa aggaataaat
     6301 gaatgaataa gtacaccatc aacgacatta cgcgcgcatc gggcggtttt gccatgctgg
     6361 cggtcgatca gcgcgaagcc atgcgcatga tgtttgccgc ggctggggca cccgctccgg
     6421 tagccgatag cgttttaact gatttcaaag ttaacgctgc aaaggccctc tcgccttatg
     6481 cctcggcgat tctggtagat caacaattct gctaccgcca ggtggttgag caaaacgcga
     6541 ttgccaaaag ttgcgccatg attgtcgccg ccgatgagtt cattcctggc aacggtattc
     6601 cggtcgatag cgtggttatt gaccgcaaaa tcaatccgct acagatcaaa caagacggtg
     6661 gcaaagcctt aaaactgctg gtgctgtggc gtagtgatga agatgcgcag caacgtctgg
     6721 atatggtgaa agagttcaac gaactgtgcc actcacacgg tctggtaagc atcattgagc
     6781 cagtcgtccg cccaccgcgt cgtggcgata aattcgatcg cgaacaagcg atcatcgatg
     6841 ccgccaaaga gctgggcgac agtggcgctg acctctacaa agttgaaatg ccgctttatg
     6901 gcaaaggtcc gcaacaagag cttctctgtg cttcacaacg tctgaatgac catatcaata
     6961 tgccatgggt gatcctctct tccagtgtcg acgaaaaact gttcccgcgt gccgtacgcg
     7021 tggcaatgac ggcgggcgca tcgggattcc tggcaggccg tgcagtctgg gcatcggtcg
     7081 tcggtttacc agacaacgag ctgatgctcc gtgacgtttg cgcaccgaaa ttacaacaac
     7141 ttggcgatat cgtcgacgaa atgatggcta aacgccgcta agatgaggat aaaaggaatg
     7201 aaatggttta acaccctaag ccacaaccgt tggctggaac aggaaaccga ccgcatcttt
     7261 gattttggta aaaattccgt agtgccgact ggttttggct ggttaggcaa taaagggcaa
     7321 atcaaagaag agatgggcac ccatctgtgg atcacagctc gtatgttgca cgtttattcc
     7381 gttgctgcgg cgatgggtcg acctggcgct tacgcgttgg ttgatcacgg tattaaagcc
     7441 atgaacggcg cactgcgtga taaaaaatat ggcggctggt atgcctgcgt gaatgacgag
     7501 ggcgtggtgg atgcctccaa acagggctat caacacttct ttgctctgct gggtgccgcc
     7561 agcgccgtca caacgggtca cccggaagcg cgcaagctgc tcgattacac cattgagatt
     7621 atcgagaaat atttctggag cgaagaagag cagatgtgcc tggagtcctg ggacgaagcc
     7681 ttcagcaaaa ccgaagagta ccgcggcggc aatgccaata tgcacgcggt ggaagctttc
     7741 ttgattgttt atgacgtcac tcacgacaaa aaatggctgg atcgcgcgat tcgcgtggct
     7801 tccgtgatta tccacgacgt cgccagaaat aatcattatc gcgttaacga acacttcgat
     7861 acccagtgga atccgctgcc ggattacaac aaagataacc cggcgcatcg cttccgcgcg
     7921 ttcggaggta caccaggcca ctggatcgaa tggggccgtt taatgctgca catccatgct
     7981 gccctggaag cccgttgcga acaaccacca gcatggctgc tagaagatgc caaaggtctg
     8041 tttaacgcca ccgtgcgcga tgcctgggca cccgatggag cggacgggat tgtttatacc
     8101 gttgactggg aaggaaaacc ggtggtccgc gaacgtgtac gttggcctat cgtcgaagca
     8161 atgggtacgg cctacgcgct ctacaccgtc actggcgatc gccagtatga aacctggtat
     8221 caaacatggt gggagtactg cattaagtac ctgatggact atgaaaatgg ttcctggtgg
     8281 caggagctgg atgcggacaa taaggtcatc accaaagtct gggacggcaa acaggatatt
     8341 tatcacctgc tgcattgcct ggtgatcccg cgtatcccgt tagcgcccgg catggctcca
     8401 gcggttgcag cgggtctgct ggatattaat gcgaaataac tgatctgaaa atagggtgcg
     8461 ggttttcccc tcaccctaac cctctcccca aaggggcgag gggaccgtcc ggtgcagttt
     8521 ttgggtttga catcagcctc acgggaggct gtatgtcgtt aattaaggtg ccttgtatgc
     8581 aaatcaccaa tatgcattgc agtggacaga ccgtttcact tgccgcgggg gattatcacg
     8641 cgaccattgt gaccgtcggt gctggtctgg ctgaattaac ctttcagggt tgtcatttag
     8701 tcatcccgca caaacccgaa gagatgccgc tggcgcatct gggtaaagtg cttattccgt
     8761 ggccgaaccg gatcgccaac ggctgttacc gttatcaggg gcaggaatat cagttaccca
     8821 ttaatgaaca tagttcaaaa gccgctattc acgggctgct tgcctggcga gactggcaaa
     8881 ttagtgaatt aaccaccacc agcgttaccc tgacagcttt tctgccgccc agttacggct
     8941 atccgtttat gctggcttca caagttgttt attcgctcaa cgcacacacg ggtctttccg
     9001 ttgagatcgc aagccagaat atcggcactg tcgcagcgcc ttatggcgtg ggtattcatc
     9061 cttatttaac ctgcaactta acatctgttg atgaatatct gttccagctt ccggcaaacc
     9121 aggtttatgc cgtcgatgaa cacgtaaatc cgacaacgct tcaccacgtt gatgagctgg
     9181 atttaaattt cacgcaggcg aaaaaaatag ccgcgacaaa aatcgaccac acctttaaaa
     9241 ccgctaatga tttgtgggaa atgaccatta cccatccaca gcaggcgctt tcggtaagcc
     9301 tttgttccga tcaaccctgg gtgcaggttt atagcggtga aaaattacag cgtcagggac
     9361 tggcagttga acctatgagt tgcccgccta acgcctttaa ttcaggtatc gacttattat
     9421 tactggaacc aggaaaaccg caccgcctgt tttttaatat ttacggacag agaaaataac
     9481 ttgtattgat tagcagtcaa aatcattgaa gaacatttca ctcgctaaag gcaacattat
     9541 gtatcttcaa tttattaagt taaaaacagc tcagcttctg taaagttttt atatatcagt
     9601 tttatgagaa tcattttaca taagcctgta ataacaggaa ggcaacaatc tcgttctcaa
     9661 ctctatggag attagttatg gatacgccac gtccacagtt attagatttt caatttcatc
     9721 agaataacga cagttttacc ctacattttc aacaacgtct tattttaacc cataacaaag
     9781 ataatccttg tttatggatt ggctcaggta tagcggatat cgatatgttc cgcggtaatt
     9841 tcagcattaa agataaacta caggagaaaa ttgcgcttac cgacgccatc gtcagccagt
     9901 caccggatgg ttggttaatt catttcagcc gtggttctga cattagcgcc acgctgaata
     9961 tctctgccga cgatcagggc cgtttattgc tggaactaca aaacgacaac cttaaccaca
    10021 accgtatctg gctgcgcctt gccgctcaac cagaggacca tatctacggc tgcggcgaac
    10081 agttttccta cttcgatctg cgcggcaaac cgttcccgct atggaccagt gaacaaggcg
    10141 ttggtcgcaa caaacaaacc tatgtcacct ggcaggccga ctgcaaagaa aatgcgggcg
    10201 gcgactatta ctggactttc ttcccacagc ctacgtttgt cagcacgcag aagtattact
    10261 gccatgttga taacagttgc tacatgaact tcgactttag cgccccggaa taccatgaac
    10321 tggcgctgtg ggaagacaaa gctacgctac gttttgaatg tgctgacaca tacatctccc
    10381 tgctggaaaa attaactgcc ctgctgggac gccagccaga actgcccgac tggatttatg
    10441 acggggtaac gctcggcatt cagggcggga cggaagtgtg ccagaagaaa ctggacacca
    10501 tgcgtaacgc gggcgtgaag gttaacggca tctgggcgca ggactggtct gggatccgta
    10561 taacctcttt tggcaagcgc gtgatgtgga actggaagtg gaacagcgaa aactacccgc
    10621 aactggattc acgcattaag cagtggtatc aggagggcgt gcagttcctg gcctatatca
    10681 acccgtatgt tgccagcgat aaagatctct gcgaagaagc ggcacaacac ggctatctag
    10741 caaaagatgc ctctggcggt gactatctgg tggagtttgg cgagttttac ggcggcgttg
    10801 tcgatctcac taatccagaa gcctacgcct ggttcaagga agtgatcaaa aagaacatga
    10861 ttgaactcgg ctgcggcggc tggatggctg acttcggcga gtatctgccc accgacacgt
    10921 acctgcataa cggcgtcagt gccgaaatta tgcataacgc ctggcctgcg ctgtgggcga
    10981 agtgtaacta cgaagccctt gaagaaacgg gcaagctcgg cgagatcctc ttctttatgc
    11041 gcgccggttc taccggtagc cagaaatact ccaccatgat gtgggcgggc gaccagaacg
    11101 tggactggag tctcgacgat ggcctggcgt ccgtagtccc ggcggcgctg tcgctggcaa
    11161 tgaccggaca tggtctgcat cacagcgaca ttggcggtta caccaccctg tttgagatga
    11221 agcgcagcaa agagctgctg ctgcgctggt gcgatttcag cgccttcacg ccgatgatgc
    11281 gcacccacga aggtaaccgt cctggcgaca actggcagtt tgacggcgat gcggaaacta
    11341 tcgcccattt tgcccgtatg actaccgttt tcaccaccct gaaaccttat ctgaaagagg
    11401 ccgtcgcgct gaatgcgaag tccggtctgc cggtcatgcg cccgctgttc ctgcattacg
    11461 aagacgatgc acatacctat tccctgaaat atcagtacct gttaggccgc gacattctgg
    11521 tcgctccggt acacgaagaa ggtcgcagcg actggacgct ctatctgccg gaggataact
    11581 gggtacatgc ctggacgggt gaagcgttcc ggggcgggga agttaccgtt aatgcgccca
    11641 tcggcaagcc gccggtcttt tatcgcgccg atagcgaatg ggcggcactg ttcgcgtcgt
    11701 taaacagcat ctaatcacat ccgcccgtgg ggaaactcat gggcaaaggg agaacatcga
    11761 tgagtcacat cacaacggaa gatccagcaa ctttacgcct gccctttaaa gagaaactct
    11821 cttacggtat tggcgacctg gcctctaaca tcctgctgga tatcggtacg ctttatcttt
    11881 tgaagtttta taccgacgtt ctggggctgc caggcaccta tggcggcatt atctttttga
    11941 tttcaaaatt ctttactgcg tttaccgata tgggtaccgg cattatgttg gattcccgac
    12001 gcaagatcgg tccaaaaggt aagttccgtc cttttattct gtatgcgtca ttcccggtca
    12061 ccttactggc gatcgccaac tttgtcggca caccgtttga tgtcaccggt aaaacggtga
    12121 tggccactat tctgtttatg ctttacggac tgtttttcag catgatgaac tgctcctacg
    12181 gcgcaatggt cccggcaatc accaaaaacc ccaacgaacg cgcctcactg gcggcatggc
    12241 gtcagggcgg cgctacgctg ggcctgctgc tgtgcacggt gggattcgtg ccagttatga
    12301 atcttatcga aggtaatcag aaacttggct atatcttcgc cgccacgctg ttttcactgt
    12361 tcggcctgct gtttatgtgg atctgctact caggcgtgaa agagcgttat gtcgaaaccc
    12421 aaccaaccaa tccggcgcaa aagcctggcc tgttgcaatc tttccgcgca attgccggta
    12481 accgcccact gttcattctg tgtattgcca acctctgcac cttaggggcg tttaacgtca
    12541 agctcgccat tcaggtctat tacacccagt acgtacttaa cgatcccatc ctgttgtcgt
    12601 atatgggatt tttcagcatg ggctgtattt tcatcggcgt gttcctgatg cctggcgcag
    12661 tcagacgttt tggtaagaag aaggtctata tcggcggcct gctgatttgg gtgctgggcg
    12721 atctgctcaa ctatttcttc ggcggcggct cggtcagctt tgtggcgttc tcctgcctgg
    12781 cgttcttcgg ctcagcgcct gaactgggcg ctggtttccg acaccgtcga gtacggcgag
    12841 tggcgcaccg gcgtgcgttc ggaaggcacg gtctacaccg gcttcacctt ctttcgcaaa
    12901 gtgtctcagg cgctggctgg tttcttcccc ggctggatgc tgacgcaaat tggctatgtg
    12961 ccgaacgtcg cacaggctga ccacaccatt gaagggttac gccagttgat cttcatctac
    13021 ccaagcgcac tggcggtagt caccattgtg gcgatgggtt gcttctacag cctgaacgag
    13081 aagatgtatg tccgcattgt ggaagagata gaagcccgta aacgcacggc gtaataataa
    13141 taaacgacgc cctgcggggc gttataagga gtgattatgt ctgaccataa tccactgaca
    13201 ttaaaactga atctgcggga aaaaatcgcc tatggtatgg gcgacgtcgg ttcgaattta
    13261 atgctctgca tcggtactct gtatctcctc aaactttata ccgatgaatt agggatgcct
    13321 gcttactatg gcgggattat ctttctggtc gcgaagtttt ttaccgcgtt taccgatatg
    13381 ctcaccggat ttttactcga ctcgcggaaa aatattgggc ctaaaggcaa atttcgtccc
    13441 tttattttat atgcagcagt tccggcggcg ttaattgcaa cgctccagtt tatcgccacc
    13501 accttttgtt tgccggttaa aacgacgatt gccaccacgc tgtttatgat gtttggactt
    13561 tcatacagtc tgatgaactg ctcgtatggt gcgatgatcc cggcaattac caaaaacccg
    13621 aatgagcgcg cgcagctcgc ggcttaccgc cagggtggtg caaccatagg gctattgatt
    13681 tgtaccgtgg cgtttattcc gttgcagtcg cttttttctg actcaaccgt cggttatgcc
    13741 tgtgcggcac ttatgttctc cattggcggc tttattttta tgatgctgtg ctacagaggc
    13801 gtcaaagagc attatgtgga cacagcgcca accggacata aagccagtat tctcaaatct
    13861 ttttgcgcga tatttcgtaa tccaccattg ctggttttat gcattgctaa cctgtgtact
    13921 ctggcggcat ttaatatcaa actggcgatt caggtctatt acacccagta tgtgctgaat
    13981 gatattaatt tattgtcgtg gatgggattc ttcagtatgg gatgcatcct cgtcggcgta
    14041 ttactggtgc cagtaactgt aaaatgtttt ggtaaaaaac aggtttattt agctggcatg
    14101 gtgctatggg cggtgggtga tatactgaat tatttctggg gaagtaactc tttcactttc
    14161 gtcatgttct cttgtgtcgc cttttttggc acggcctttg taaacagcct gaactgggca
    14221 ctggtaccag ataccgtaga ttacggtgaa tggaaaaccg gtattcgtgc cgaaggttct
    14281 gtttataccg gttatacctt ctttcgtaaa atttctgccg cacttgctgg cttcttgcca
    14341 ggcattatgc tgacgcaaat tggttatgtt cccaacatcg cgcaaagcga tgcgacatta
    14401 cagggtttgc gtcagctcat ttttatctgg ccttgtgcac tggcaattat tgctgcattg
    14461 acgatgggat tcttttacac actcaatgaa aaacggtttg cattgattat tgaggaaatc
    14521 aaccaacgca aaaataaaga gatggcgaca gaaaaaaaaa cggcttccgt aacgttataa
    14581 atgtcaatag ccgctatttc catcctggtg ggtggcggcc tccctacgtt taaaaaatgg
    14641 acttattatg aaaaagatta atgcaataat tttattatct tctctgacct ctgcctcggt
    14701 atttgctggc gcatacgtcg aaaacaggga ggcatataat cttgcctccg atcagggcga
    14761 ggttatgcta cgtgtgggtt ataacttcga tatgggcgcg ggtattatgt taaccaatac
    14821 ctacaccttt cagcgagaag atgaactaaa acatggctat aatgaaattg aaggttggta
    14881 tcctttattc aaaccaaccg ataaattaac catccagccc ggtggcttaa ttaatgataa
    14941 gagtatcagt tcgggtggtg cagtgtatct ggac
//
LOCUS       DAOFMX010000092        14859 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.092, whole genome shotgun sequence.
ACCESSION   DAOFMX010000092 DAOFMX010000000
VERSION     DAOFMX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.092"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(92..517)
                     /gene="arsC"
                     /locus_tag="QBA20_003382"
     CDS             complement(92..517)
                     /gene="arsC"
                     /locus_tag="QBA20_003382"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDQ6293803.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(530..1819)
                     /gene="arsB"
                     /locus_tag="QBA20_003383"
     CDS             complement(530..1819)
                     /gene="arsB"
                     /locus_tag="QBA20_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDQ6293804.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1873..2226)
                     /gene="arsR"
                     /locus_tag="QBA20_003384"
     CDS             complement(1873..2226)
                     /gene="arsR"
                     /locus_tag="QBA20_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDQ6293805.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2969..3052
                     /locus_tag="QBA20_003385"
     CDS             2969..3052
                     /locus_tag="QBA20_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDQ6293806.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3106..4458)
                     /gene="gorA"
                     /locus_tag="QBA20_003386"
     CDS             complement(3106..4458)
                     /gene="gorA"
                     /locus_tag="QBA20_003386"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDQ6293807.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4530..5372)
                     /gene="rlmJ"
                     /locus_tag="QBA20_003387"
     CDS             complement(4530..5372)
                     /gene="rlmJ"
                     /locus_tag="QBA20_003387"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDQ6293808.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWQLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5575..7617
                     /gene="prlC"
                     /locus_tag="QBA20_003388"
     CDS             5575..7617
                     /gene="prlC"
                     /locus_tag="QBA20_003388"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDQ6293809.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7625..8377
                     /gene="rsmJ"
                     /locus_tag="QBA20_003389"
     CDS             7625..8377
                     /gene="rsmJ"
                     /locus_tag="QBA20_003389"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDQ6293810.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8426..9895)
                     /gene="dtpB"
                     /locus_tag="QBA20_003390"
                     /pseudo
     CDS             complement(8426..9895)
                     /gene="dtpB"
                     /locus_tag="QBA20_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10213..10647)
                     /gene="uspA"
                     /locus_tag="QBA20_003391"
     CDS             complement(10213..10647)
                     /gene="uspA"
                     /locus_tag="QBA20_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDQ6293811.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11041..11376
                     /gene="uspB"
                     /locus_tag="QBA20_003392"
     CDS             11041..11376
                     /gene="uspB"
                     /locus_tag="QBA20_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDQ6293812.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11447..12946)
                     /gene="pitA"
                     /locus_tag="QBA20_003393"
     CDS             complement(11447..12946)
                     /gene="pitA"
                     /locus_tag="QBA20_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDQ6293813.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13178..14377
                     /locus_tag="QBA20_003394"
     CDS             13178..14377
                     /locus_tag="QBA20_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDQ6293814.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3699 a         3781 c         3915 g         3464 t
ORIGIN      
        1 acggttttgt aggtcggata agacgcgtta gcgtcgcatc cgacataaac aaagcgcact
       61 ttaccaacta tcggcagtgg atatcgccgc tttatttcag gcgcttaccc gcttcatcaa
      121 ccactttctc gccatcttcc ttggagaatg cgcctttttg cgcatctggc agaatttcca
      181 gcaccacttc tgaagggcgg cacaggcgag ttcccagcgg cgtcaccaca atcgggcgat
      241 taatcagaat cgggtgctga agcataaagt cgattaaccg atcgtcagta aatttatctt
      301 ccgcaaggcc cagctcctca tacggttcga cgtttttacg cagcagcgcg cgtacggaaa
      361 tccccatatc ggcaatgagt ttgaccagtt catcgcgcgt tggcggagtt tccagataat
      421 ggataatggt cggttctgtg ccgctgttgc ggatcatctc cagcgtatta cgcgacgtgc
      481 cgcaggccgg gttgtgataa atggtaatgt tgctcatatc agtatctcat tacaaagtga
      541 aagagagacg tagcgccagc gcagccagcg tcacaaacag cacaggcagg gtcatgataa
      601 tccctgtacg gaagtaatat ccccagctga tagtcatatt cttctgcgaa agtacgtgca
      661 gccagagcag cgttgccagg ctaccaatcg gggtaatttt cggccccaaa tcgcagccaa
      721 tcacgttggc ataaaccatc gcttctttga taacgccaga tgccgtgctg ccatcaatgg
      781 acaacgcgcc aaccagtacc gtcggcatat tgttcataat agaagagagg aaggcggtga
      841 ggaatccggt gccgagcgtc gcggcccaca ggccgttatc cgccagcacg ttgagtacgc
      901 cagaaagata ttccgttaat ccggcattgc gcaggccata aaccaccaga tacatgccga
      961 gcgagaagat gacaatctgc cagggggcac cgcgcaggac tttacccgta ttaatcgcat
     1021 gaccgcgttt agcgacgaca aataatatca gtgcgcccac agctgcaatg gcgctcaccg
     1081 gaatgccgag cggttccagg acgaaaaatc ccaccagcag aagcagtaaa acaacccagc
     1141 cagttttgaa cgtagcagga tctttgatcg cttctgcggg agatttcagc agcgccatat
     1201 cgtagttctg cggaatatct ttgcgaaaat agagatgtag catcaccagc gtggcaacaa
     1261 tcgcggcgat atccaccggc accatcaccg aggcgtattc gcgaaagccg aggccaaaga
     1321 aatcagcgga aacgatattc accaggttgg agacaataag cggcaggctg gcggtatcgg
     1381 caatgaatcc ggccgccatc acgaacgcca gcgtcgtgcc tttactgaat cctaaagcca
     1441 gcagcatggc gatgacaatc ggcgtcaaaa taagcgccgc gccatcattg gcaaacaggg
     1501 ccgcaacggc ggcaccgagc aggacaatcc aggtaaacag caagcgaccg cgtccattgc
     1561 cccagcgtga aacgtgcagc gctgcccatt caaaaaagcc ggactcatcc agcagcaggc
     1621 tgatgataat gacggcaata aacgccgccg tcgcgttcca gacgatattc cacaccaccg
     1681 gaatatcacc cggatgaacc acgcccgtaa ccaacgccag taccgcgccg agcgttgcac
     1741 tccagccgat gcctaaacct ttcggctgcc agataaccaa tacgatggtc aggacaaaga
     1801 tagcgcctgc cagtaacata atgcctcccg gataaaacac atgtgcaaat ccatatgtgt
     1861 ttagctaaat ttttaactgc aaatgttctt actgtctccg gaacagtttt gtctggccag
     1921 gttgcggaca attgcctgaa tcttttcctg ttcacatcgc caggcttcat caataatttt
     1981 cgccgcccat gctggaatat gcggcgataa gcggtaatga acccacttac cttgcttgcg
     2041 gtccagcaat agcccgcttt cacgcagcaa tgccagatgg cgggatatct tgggctgcga
     2101 ctgatcgagg gcagtgcaga gatcgcagac gcataactct cccagttcgc taagcagtaa
     2161 aacgatgccc agacgggttt catcagcaag aattttgaac aattggatgg gtaacagaaa
     2221 tgacatattg cgctcctgat tgttgcaggt agtgtctctc ttcgaagcgg ataagtcaaa
     2281 aacatatatg acttaacgaa tgtgtaagtg cagaggaagg taataggtgt gaattttgag
     2341 ttggctattc atttgaaagg cggtttgaat caggacgtaa aaaaaccgac tttgcgtcgg
     2401 ttttttactc ttcagcctga gttggtggct ctggctggag tgagaatgcc cacatctaac
     2461 ggttcaatac tattagccag tggctaacat tgcaagcgtt agcgatttct tcagctggag
     2521 aagggacagt ttgctataca tggtggtgaa agaggattta tatactttca tcgggtcgct
     2581 gccgtaccaa gccttgtccg aatccggacg taaaaaaacc gacttcgcgt cggttttttt
     2641 actcttcagc ccagagttgg tggctctggc tggagtgaga atgccatcat ctaacgcatc
     2701 aatgctaata ccatactgaa actattgcaa ggttatgctg gttatttagc ctaattacac
     2761 tgtatgatta tccagttagc tctgaggcat tttcactctg gcaatgcgca taaacgcttt
     2821 caaagtcctg gtcagaagta cgggtggtgc cgttaactga tgctctggcc ggagtgagat
     2881 tgcccacatc taacaatgag gcatgcgccg tgacaggcag tggatgagta agcggatgca
     2941 ttctcactcc atcgcatgga gaaaacgggt gattgataaa gcaatcatcg ttcttggggc
     3001 gttaattgcg ctgctggaac tgatccgctt tctgcttcag cttctgaact gatagcggaa
     3061 acgtaattaa gggctaagag cacactactc ttagcccttt aacatttaac gcatggtcac
     3121 gaactcttct gccgccgttg ggtgaatggc gacggtattg tcgaagtctt ttttggttgc
     3181 gcccatcttc agcgccaccg cgaagccctg caacatttcg tccataccaa agccaatgcc
     3241 gtgaataccg acaatcttct cttctggccc aacgcacacc agcttcatgc ggcacggctg
     3301 gcggtgagtg gtgacggcgg tatacatcgc ggtgaaagag gatttataca ctttcacctg
     3361 atcgtcgccg tactgctcgc gcgcctgcgg ttccgttaaa ccaacagtac caatcggcgg
     3421 atggctgaag accacggtcg gaatgttgct gtaatccaga tgctcatccg gcttgttatt
     3481 aaacaggcgt tcagagagac ggcgacccgc tgcaactgcc accggtgtca gttccactgc
     3541 acccgtgtta tcgcccaccg cgtaaatacc ttcaatattg gtgttttgat atttatcgac
     3601 gacgatatag cctttttcgt tggttttaac gccagcggct tcgaggttga tgttgtcatt
     3661 ggcaggctcg cgaccaatcg cccaaatcag gcaatccacc gtttcactgc gaccatcttc
     3721 cagctccagc gtcaggctac catcggcatt tttcactacc gctttcggga tggcgttggt
     3781 atgcaactgt gggccttcgg cgttcatcac ttcgaccagc gtttcagaga tcatcgggtc
     3841 gaagctgcgc agcggcgcat gtttacgcac aaacagatgc gttttcgcgc cgaggccgtt
     3901 aatcacgcca gccagctcaa cggcgatgta acccgcgcca acaaccgcca cgcgctctgg
     3961 caaagcagga agggcgaaga agccatcgga atcaataccg tattccacgc ctggaatatc
     4021 cgggtggctc ggacgaccgc cggtggcgat cagaatatga tcggccgtga tggtttcgcc
     4081 gttaacttcc agcgttttgg catcaacgaa gcgggcaaag cctttgatta catcaacgtt
     4141 atttttaccg agcacgtttt cataggaagt atgaatacgg tcgatatagg cggtacggct
     4201 ggcgatcaac gtttcccagt tgaacttgtt gatggtggta tcgaaaccat aatcagggcc
     4261 gtacatatgg atcgcttcac ggatttgcgc cgcgtgccac atcacttttt tcggcacgca
     4321 gccgacgttc acgcaggtgc cgcccagctc tttggcttca atcagtgcgc atttctggcc
     4381 gtacatagcc gcgcggttga tggaggcgat accgccgctg ccgccgccga tggcgatgta
     4441 atcatagtgt ttagtcatga catattgtcc ttatcgttga ttaccgcgat tgtagcgcga
     4501 gacgtaatag gtgccagcaa tggctgcaat tactccggca cgatccagct tacggtggcg
     4561 tgcccggtac ctgccggaac cagtttgctg tgcagccacg acagcacgtt attcatctgc
     4621 tgttccagct gccacggcgg gttaatcaca atcatgccgg aagcggtcat gccacggcga
     4681 tcgctgtctg gcagcaccgc cagttcaatt tgcagaattt tgcgaatgcc ggtcgcttcc
     4741 aggtcgtgga tcatgcgctt aatttgctgg cgcagcacca ccggatacca caatgcgtaa
     4801 gtgccagtgg cgaaacgttt gtaaccttct gctatcccgc tgaccaccgc ctgatagtcg
     4861 gttttcattt catacggcgg gtcgataagg attaaaccac ggcgagaaac cggcggcagt
     4921 ttggccttaa gttgctggaa accgtcggct ttttcgacgc gtgcacggct gtctttctga
     4981 aatccagaac gcagcaacgg gtaatcgctc gggtgcagtt cggtcagttg caggctgtct
     5041 tgttcgcgca gtagctggcg agcaatcaac ggcgaaccgg ggtagtaacg caactggccg
     5101 ctacggttga agtgttttac cacattgatg tacgcctcca gttctgcggg caaatcgtcc
     5161 tgctgccaga tacgggcgat accttcgaga tattcgccgg tacgctcggc atgttcgcta
     5221 cctaactgat aacgcccggc ccctgcgtgg gtgtcgagat agagaaacgg tttatctttc
     5281 tctttcagcg actcgatgat caggctctga acggtatgtt taaggacgtc ggcgtggttg
     5341 ccagcgtgaa agctgtggcg ataactgagc atgggtaaag gtgttccggt aagtaaaaat
     5401 tggcccgtat tcggggcgca caaaagccta tcaggacagt ataccgaaaa gaggccgccg
     5461 ccgcgaaagc gtaacgtttc tcattgaaat tcactacact taaccccatg ctacacacat
     5521 tatgtaaagc gcctgttgag cgcttcctta acctctttaa ccaggactgc gctaatgacg
     5581 aatccgttac tgactccctt tgaattgcct ccgttttcta aaattctccc ggaacatgtc
     5641 gttccagccg tgactaaggc attgaacgac tgccgcgaaa atgtggagcg cgtagtagcg
     5701 caaggggcac cgtacacctg ggaaaatctc tgccagccgt tggcggaagt ggacgatgtt
     5761 ttggggcgta tcttctcccc ggtcagccac ctgaactcgg tgaaaaatag cccggaactg
     5821 cgtgaagcgt acgaacaaac cctgccgctg ctgtcggaat acagcacctg ggtagggcaa
     5881 catgaagggc tgtataaagc gtaccgcgac ctgcgcgatg gcgatcatta cgccacgctg
     5941 aacacggcgc agaagaaagc ggttgataac gccctgcgtg attttgaact ctctggcata
     6001 ggtctgccga aagagaaaca gcagcgttac ggcgaaattg ctacccgtct ttctgaactg
     6061 ggcaaccagt acagcaacaa cgtcctcgat gcgacgatgg gctggaccaa actcgttacc
     6121 gacgaagcgg ggctggcggg gatgccagaa agcgcgctgg ctgcggcaaa agcccaggcc
     6181 gaagcgaaag agctggaagg ctacctgctg acgctggata tcccaagcta cctgccggta
     6241 atgacctact gcgacaacca ggctctgcgc gaagagatgt atcgcgctta cagcacccgc
     6301 gcctccgatc aaggcccgaa tgccggtaag tgggataaca gcaaggtgat ggaagagatc
     6361 ctcgcgctgc gtcacgaact ggcgcaactg ctgggctttg aaaactacgc ctttaaatcc
     6421 cttgccacta aaatggcaga aaacccgcag caggtgctgg atttcttaac cgatctggca
     6481 aaacgcgcgc gtccgcaagg cgaaaaagag ctggagcaac tgcgtgcctt cgctaaagcc
     6541 gaatttggtg tcgatgagtt gcagccgtgg gatatcgctt actacagcga aaaacagaaa
     6601 cagcacctct acagcatcag cgacgagcag ctgcgtccgt acttcccgga aaacaaagcg
     6661 gttaacggcc tgtttgaagt ggtgaaacgt atttacggca tcaccgctaa agagcgtaaa
     6721 gatgttgatg tctggcatcc ggatgtacgt ttcttcgaac tgtatgacga aaataacgaa
     6781 ctgcgcggca gcttctacct cgatctgtat gcccgtgaaa acaagcgcgg cggggcgtgg
     6841 atggatggct gcgtaggcca gatgcgtaaa gctgatggtt ctctgcaaaa accggtcgcg
     6901 tatttgactt gtaacttcaa ccgcccggta aatggtaaac cggcgctgtt cactcacgac
     6961 gaagtgatca ccctgttcca cgagttcggt cacggcctgc accatatgct gacccgcatc
     7021 gaaaccgctg gtgtttccgg tatcagcggt gtgccgtggg atgcggtcga actgccgagt
     7081 cagtttatgg aaaactggtg ctgggagccg gaggcgctgg cgtttatctc tggtcactat
     7141 gaaaccggcg aaccgctgcc gaaagagttg ctggataaaa tgctggcggc gaagaactac
     7201 caggcggcgc tgtttattct gcgtcagctg gagttcggcc tgtttgattt ccgccttcat
     7261 gccgagttcc gcccggatca gggggcaaaa atcctcgaaa ctctggcaga aatcaagaaa
     7321 ctggttgccg tggtgccatc tccgtcctgg ggccgtttcc cgcacgcttt cagccatatt
     7381 ttcgccggtg gttatgcggc aggttactac agctacctgt gggctgacgt actggcggca
     7441 gatgctttct cgcgctttga ggaagagggc attttcaacc gtgaaaccgg gcagtcgttc
     7501 ctcgacaaca ttctgagccg tggcggttca gaagagccga tggatctgtt caaacgcttc
     7561 cgtggtcgtg aaccgcagct ggatgcgatg ctggagcatt acggcattaa gggctgatca
     7621 ttcagtgaaa atctgcttaa ttgatgaaac aggcgccgga gacggcgcct tatctgttct
     7681 ggcggcccgc tgggggctgg agcacgatga agacaacctg atggcgctgg tgttaacgcc
     7741 ggaacatctg gaattgcgca agcgtgatga gccaaaactt ggcggcatct ttgttgattt
     7801 tgttggcgga gcgatggcgc accgacgcaa attcggcggt ggtcgcggtg aggcggtggc
     7861 gaaagcggtg ggcattaaag gcgattattt gccggatgtg gtggacgcca ccgcagggct
     7921 ggggcgcgat gcctttgtac tggcttcagt cggctgccgc gtgcggatgc tggagcgtaa
     7981 tccagtggtt gccgcgctac tcgacgacgg cctggcgcgt ggttatgcgg atgcggaaat
     8041 cggcggctgg ttgcaggagc ggttgcagtt aattcacgcc tccagcctga cggcgctaac
     8101 cgatattacc ccacgcccgc aggtggtcta tctcgatccg atgttcccgc ataagcagaa
     8161 aagcgcgctg gtgaaaaaag agatgcgtgt ctttcagtcg ctggtggggc cggatcttga
     8221 tgccgatgga ttactggagc ctgcacgctt gctggcgacc aaacgcgtgg tggtaaagcg
     8281 cccggactac gcgccaccgc tggcgaatgt cgccacgcca aacgcggttg tgactaaagg
     8341 gcatcgcttt gatatctatg caggtacgcc agtgtaaaag taaagcaggg tagcgataac
     8401 gctaccctgc tttctgcaag aataattaat ggctttccgg cgtcgcaatc atgcgtttca
     8461 gccacggcac catcaacagc atcactactg caacgcccag cgtgaccaga ccaatcttac
     8521 caaacacgtt ggtatagacg ggcaacgtct caagcggatc ggtaatgttg tccggcaccg
     8581 cggtaaatgt tgccacatag ccgcccagca agaacgcggc agcctgcgtc aggaaccaca
     8641 tcccgagaat aaagcccatc aaatgctgcg gcaccagggc agcaatcatc gccaggccaa
     8701 gggcgctaat aaacaattca cccaggctct agaataaata caccagcacg ataaaccatg
     8761 gcgatgtcag cccctggtca tccgcaaacc acattcccgc agcagcagcc gtcaaaaagc
     8821 ccagtgagca cataaacatg ctgagagtaa atttcatcgg catcgagagg tctttgcctt
     8881 tgttacccag atgcgtgtaa atgcctgcca gtattgggct ggcgagtacc acccagaacg
     8941 ggttaagcgc ctggaagctg accgggttga tggaaaaacc gagaatttca tgatgcacgt
     9001 tgttgatggc aaagaagttc agcgatgttg gcatctgggc gtagagaatg taaaacacca
     9061 ccgcttcgag catcaggaca aaggcgacaa acattttatt gcgcccggtt ttatccagct
     9121 tgaatgcctg acgaaagaag atgatagtga cgacgatgga gagaacaatc agcaccagat
     9181 tggcgacttc tacgttgtgc atcagccatg cacatacgaa gatcatcacc acgctgccaa
     9241 gtaatacgta caacagtttg ctgaagctca tcggcttgaa gtcgggttca gaaccaatgt
     9301 ctttcaccat tccacgacag gcgatgtaaa ccagtaatgc gataattaac cccgccccgc
     9361 acaggttgta ggtgactgaa taaccgaatc tatcagcgat cacaggggcc agcgataacg
     9421 ctatcaacga gccgatattg atcgacatat agaacagggt gaatgcgcca tcaagccgcg
     9481 gatctttcgg cggatagcac ttcgaaagca agctggctgg gttagcttta aacaggccgt
     9541 taccgacagc gatagtcccc agggcgatga aaatcaggtc aggcttaagt agcgacatgc
     9601 cggtcatgaa gtagccaatc gccagcacaa gtgctccaag aacaatggtg cgtttggtcc
     9661 ccaacaggtg gtcgccgaca tagccgccaa tggaaatgag gccatagacc agtgcagcaa
     9721 aagcaccaaa agtgacaaaa gcctgctctt gcgagaatcc aagctgttta acgaagaaaa
     9781 ccgccagtac gccctgcacg ccgtagtagc cgaatcgctc ccataactcg acaaaaaaga
     9841 tcatgaaaaa tgggcgaggt tgctgcagca tccccatggg tgttgttgta ttaatattta
     9901 ttaaccttcc aataccatcc tgaaaagtat gaacgcgtaa gggataacgc gcgaaaaagt
     9961 ttacatctgt cagagattct taatattgat gcggtgaata gattaataca ctattacaac
    10021 agaaaataac cagctgatca cactaatgat ggaaaatatg atggtgttaa attttgtatt
    10081 ggcaggagat tttgattgcg tacgcagatc gtaggccaga taaggggttt acgctgcatc
    10141 aggtaacaag gcgcgccaaa aagcaaaacg cccgcatatg cgggcgttca ggaacacgtc
    10201 gtggagggat gattattctt cttcgtcgcg tagcggaaca atcagcatat caacgtgaac
    10261 ggtgttgatc agctgacgtg cggaagacat cagtttgctc cagaagtcct ggtggtgacc
    10321 acaaaccacc agatccatat cgtatttctt gattgcatcg accagaacct ggcctaggtc
    10381 gccgctgccg ctcagggttt cagtgattgg atagcctgca ttagtggaaa gctcggtcag
    10441 cgcatgatgt gtctcttcag agatgcgttt ctgcatatca cccagattca catcaataag
    10501 cccggtgtat aggtcagagt agtttacatc tacgtggatc agagaaactt tcgcattgta
    10561 ggggcgagcc atagagactg ctttctctac cagaactttg ctttccgggg agagatcgac
    10621 tgcgatgaga atgtgtttat aagccatagt gttactcctt ccataaagtt gtcgatgact
    10681 ggccagctag cgtttcttgt gccgttacgt cacccgcgtc ctgcgaacta cgtgcgcggg
    10741 gctaacccag ccgtaaagat tcagtgttaa gaccatcctt accttatagc gacccggatg
    10801 atccgtcaat ccgccttgct tacccgttag ataaacaaat tttcgacatg aatattatat
    10861 tgatagtggt taaccttctg gaaaaaaaac aacctgatct cctacactat ctatagagcc
    10921 gctcggatgt tggtcaccac aaaagcggtt tctccaggac ctggtgttaa ctgattggca
    10981 gcgtatcggg agcggtagcc ccggggtgga actccgcggg caggtcgccg gggaggaggt
    11041 atgataagca ccgtcgcatt attttgggct ttatgtgtcg tttgcattgt taacatggcg
    11101 cgctatttct catcactacg cgcgttgtta gtggtactgc gtaactgcga tccattgctc
    11161 tatcaatatg ttgatggagg gggctttttt acctcacatg gccaacccaa caaacaggtg
    11221 cgtctcgttt ggtatatcta tgcccaacgt tatcgcgatc atcacgatga tgagtttatt
    11281 cgccgctgtg agcgggtgcg tcggcagttt attctgacta gcgcattgtg tggtctggtg
    11341 gtggtcagcc tgattgcatt gatgatttgg cattaagcac ttcagcaata ataacgtata
    11401 aaaaaagcgg gccagtaaac tgacccgctc gttttggtgc gtacgattac aggaactgca
    11461 aggagaacca gtacagcccg ccggaaagta gtaccgcagc cggaagggta aacacccagg
    11521 ccatcagaat actggttacg gttttacgct gtaagccgcc gccatctacc accatcgtcc
    11581 ccgcgacaga agaggagagt acgtgagtgg tggaaaccgg catcccggta taactcgcca
    11641 ggccgattga cactgccgcc gtcatctggg cagacatccc ctgagcgtag gtcatgcctt
    11701 tcttaccgat tttctcaccg atagtcgttg ccacacgacg ccagccaatc atcgtaccga
    11761 tacctaacgc cagcgcgacc gccatgatga tccacaccgg cgcatactcg atggtgctaa
    11821 gcatgtcgga cttcagtttc ttcaacaggc gctgatcgtc agcactcaca ccaggcatct
    11881 tcaccacttt gtcgatagtg tcagaaacgc acagcataat gcggcgcatc tggctacgtt
    11941 gatcaagcga cagcttgtcg tagctttcca catcggtggt caacatacct ttcaggcggt
    12001 tgagcgcgtt aatggtattc gacggatggc agtggaactc cgcaggttgc gttgcgccag
    12061 cttccggagc cggtactaac tgatcagcac cggtagcctg tttgagcagc gcaggatgct
    12121 gctcaaagta agcttcgacg ttgttgatgg catcacgggt acgggtgatt tcgtagccag
    12181 tggcattcat gttcaccacg aagcctgctg gcgcgacgcc aatcaatacc aacataacca
    12241 gaccaatgcc tttctgacca tcgttcgcgc cgtgcgaaaa cgccacgccg atagcggaaa
    12301 ggatcagcgc gatacgcgtc cagaacggcg gctttttctt gccgtctttc ttttcacgct
    12361 ccgctggggt caggtggata cgggcgcgtt tcttggtgcc gctccagtag cgacgcagca
    12421 agaaaatcag accgccagca aacaccaggc cgacaatagg ggaaacgatc agagaaccga
    12481 aaatacttaa tactttcggg atattgagtg catccaccac tgacgtcccg gtcatcaacg
    12541 cattggttaa accaatcccg atgatcgcgc caatcagcgt atgagagctg gatgcaggta
    12601 aaccaaagta ccaggtaccc aggttccaga taatcgccgc cagcaacata gagaacacca
    12661 tggcaaggcc atgagacgat cccatattaa gcagcagatc cgtcggcagc atatgcacaa
    12721 tggcataggc aacactcaga ccacccagca aaacacccaa aaagttgaac accgccgcca
    12781 taaccacggc gagctgagaa cgcatcgcgc gggtatagat aacggttgcc acggcgttgg
    12841 ctgtgtcatg gaaaccattg atggcttcgt agaacagcac aaaagccagt gcaagcaata
    12901 ataacagccc ggtatgcaaa tccaggccag caaacaaatg tagcatagga cgttacgcca
    12961 ttttgaggac atgaacgcgg cgcattatca gtgactttcg cggcgcgggc aaagtgaaat
    13021 atagactttt tttgatttgc ctcctgtatg gatttcactc aaaaaataat tatcttatat
    13081 aattcaggca aatacttcct tttagtaata ttgatgctgg tgcgaccact gaggaatctt
    13141 tacaattcac gcccgttttt tctaagagga gcgcaacgtg gaaaggtttg atgccattat
    13201 tataggcgct ggtgcggcgg gtatgttctg ttctgcgctg gcaggtcagg taggacgccg
    13261 ggttctgctg atcgataatg gtaaaatact agggcgcaaa atccttatgt ctggcggtgg
    13321 gcgctgcaac tttaccaacc tttatgtcga accaggcgct tatctgagcc agaatccgca
    13381 tttttgtaag tctgcactcg cacgttttac ccagtgggat ttcattgatc tggtcaataa
    13441 acacggtatc gcctggcacg agaaaacgtt agggcaactc ttctgcgatg actccgcgca
    13501 gcagattgtc gacatgctgg tggatgagtg cgagaagggc aatgtaacct tcagattgcg
    13561 tagcgaagtg ctgagtgtgg cgaaggatga aacaggcttc acgcttgatc tgaacggcat
    13621 gactgtcggt tgcgaaaagc tggtcatcgc gaccggtggg ctgtcaatgc cggggctggg
    13681 cgcatcgccg tttggttata agattgccga acaatttggc ctcaacgtgc tgccgacccg
    13741 cgcgggtctg gtgccattca ctctgcataa accgttgctc gaagagttac aggtgctggc
    13801 gggcgtggcg gtgccttccg tgattaccgc tgaaaacggc accgttttcc gtgagaactt
    13861 actcttcacc caccgcggct tatctggacc ggcggtgttg cagatttcaa gctactggca
    13921 accgggtgaa tttgtcagta tcaatctgct accggatgtg gacctcgaaa ccttcctgaa
    13981 tgagcagcgt aacgcacatc cgaatcaaag cctgaaaaac acactggcgg ttcatctacc
    14041 gaagcggttg gttgaacgct tacagcaact cgggcaaatc ccggatgttt cgctaaaaca
    14101 gctcaacgtg tgtgaccaac aggcactgat tagcacattg accgactggc gcgtacaacc
    14161 caacggcact gaaggctatc gcactgccga agtgacgctc ggcggcgtgg acaccaacga
    14221 actttcttca cggacgatgg aagcgcgcaa agtgcctggg ctgtacttca tcggcgaagt
    14281 gatggacgtt accggctggc tggggggcta taacttccag tgggcgtggt cgagtgcatg
    14341 ggcttgtgcg caggatttgg cgtcaattgt gaaataacca tgacgttttc ccgtcagccg
    14401 tttgtatatg tcgttattaa gtcattttgg tacggttctg catccgacat tctggtgtta
    14461 actcgctgtt ttgttagcga attaacgcta ttgccgttgt ggttatttta tagtgtattt
    14521 attctcagag aatatatatt actgaaataa aaaacacaaa gaataaccaa aggaaattat
    14581 ttgaaacgta aaaggtatat atttttaatc tgtaaaattt aaaatcacct cctctggatt
    14641 gaatcaactt gctctattta agacgattgt ttttacattt tcaccacctg tatcaggtgg
    14701 tgttttttta ttggcggcaa tgcctttatc ttatttttat tacgttgtaa atatggctat
    14761 tcctaaatga aatttgattt gctcagttta tttgtgatgt caggggagtg cttgccatga
    14821 gagtcgtgat attattatgc atcattttta ggtaatgct
//
LOCUS       DAOFMX010000093        14480 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.093, whole genome shotgun sequence.
ACCESSION   DAOFMX010000093 DAOFMX010000000
VERSION     DAOFMX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.093"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(114..380)
                     /locus_tag="QBA20_003395"
     CDS             complement(114..380)
                     /locus_tag="QBA20_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDQ6293815.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            654..4178
                     /gene="nifJ"
                     /locus_tag="QBA20_003396"
     CDS             654..4178
                     /gene="nifJ"
                     /locus_tag="QBA20_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDQ6293816.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4545..5674
                     /gene="ompN"
                     /locus_tag="QBA20_003397"
                     /pseudo
     CDS             4545..5674
                     /gene="ompN"
                     /locus_tag="QBA20_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5815..6249
                     /gene="uspF"
                     /locus_tag="QBA20_003398"
     CDS             5815..6249
                     /gene="uspF"
                     /locus_tag="QBA20_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDQ6293817.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6425..7360
                     /gene="ttcA"
                     /locus_tag="QBA20_003399"
     CDS             6425..7360
                     /gene="ttcA"
                     /locus_tag="QBA20_003399"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDQ6293818.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7489..8856)
                     /gene="dbpA"
                     /locus_tag="QBA20_003400"
     CDS             complement(7489..8856)
                     /gene="dbpA"
                     /locus_tag="QBA20_003400"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDQ6293819.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(8886..9059)
                     /locus_tag="QBA20_003401"
     CDS             complement(8886..9059)
                     /locus_tag="QBA20_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017372341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293820.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(9334..10317)
                     /gene="zntB"
                     /locus_tag="QBA20_003402"
     CDS             complement(9334..10317)
                     /gene="zntB"
                     /locus_tag="QBA20_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDQ6293821.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10572..11804
                     /gene="dgcM"
                     /locus_tag="QBA20_003403"
                     /pseudo
     CDS             10572..11804
                     /gene="dgcM"
                     /locus_tag="QBA20_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11825..12388)
                     /gene="smrA"
                     /locus_tag="QBA20_003404"
     CDS             complement(11825..12388)
                     /gene="smrA"
                     /locus_tag="QBA20_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDQ6293822.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12642..14096)
                     /locus_tag="QBA20_003405"
     CDS             complement(12642..14096)
                     /locus_tag="QBA20_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDQ6293823.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMASIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14093..>14480)
                     /locus_tag="QBA20_003406"
     CDS             complement(14093..>14480)
                     /locus_tag="QBA20_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDQ6293824.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3863 a         3651 c         3420 g         3546 t
ORIGIN      
        1 aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta
       61 acgctggcga ccgcgaaaca gacattaact tataagctgg cggatttaat gaattaatag
      121 ctgccacagc tcccggcggc aagtgactgt tcgctacagc gtttgccgtt gggtaatgca
      181 cacatcccaa tcgccgtacc atccagttga cgggcaacag aaagcgaacc gccgatcatt
      241 gcacaatttg cttctccact actggacatc gacgctttta aacttggcgc tacgtgcgcc
      301 gcagtcgcct gctgaacagg ctcactacta cacgccgaca acaataaagc ggcacaccct
      361 acccaaaacg ctgctcgcat ctcttcttcc tctgatcttc aagccaaacg acaccgccat
      421 aaataatagg cagcacagag ggcggcgtcg agagctgtcc tgcgcgttgc cccgcatttt
      481 tactttttta tggctatttt tttgccctct gtttgatcaa aacattcatt actctgatgt
      541 gggggacaca aaagcgaaaa tgcagaagaa agccatttgc taaaattgaa ggattactac
      601 tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat gtaagggtgt catatgatta
      661 ctattgacgg taatggcgcg gttgcttcgg tgacgtttcg catcagtgaa gttatcgcca
      721 tctaccctat tacccccagt tccacgatgg cagaacaggc tgatgcctgg gccggaaacg
      781 gcttgaagaa cgtttgggga gacacaccac gcgtggttga aatgcagtcg gaagcgggtg
      841 ctatcgctac cgtgcatggc gctttgcaga cgggtgccct ttcaacatcg tttacgtcat
      901 cgcagggttt gctgctgatg atcccgacgc tgtacaaact ggcaggcgaa ctgacgccat
      961 ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc actttctatt tttggcgatc
     1021 attccgacgt tatggcggtg cgccagacgg gttgcgcgat gttgtgtgcg gctaacgtcc
     1081 aggaagcgca agactttgct ctcatttcgc acatcgcaac gctgaagagc cgcgtgccat
     1141 ttattcattt ctttgatggt tttcgcacgt cccacgaaat caataaaatt gtcccgctgg
     1201 ccgatgacac tattcttgat ctcatgccgc aggccgaaat tgatgctcat cgcgcccggg
     1261 cgctcaaccc ggaacatccg gtgatccgcg gtacgtccgc caatcctgac acttatttcc
     1321 agtctcgcga agccaccaat ccctggtaca acgcggtcta tgaccatgtt gaacaggcga
     1381 tgaatgattt cgctgccgcg acaggtcgtc agtatcagcc gtttgagtat tacgggcatc
     1441 cgcaagcgga acgggtgatt atcctgatgg gctctgccat tggcacctgt gaagaagtgg
     1501 ttgatgaatt gctaacccgt ggcgaaaaag ttggcgtgct gaaagttcgt ctgtaccgtc
     1561 ccttctccgc caaacacttg ctgcaagctc tgccgggatc cgtacgcagc gtggcggtac
     1621 tggacagaac caaagaaccc ggtgcccagg cagaaccgct ctatctggat gtgatgaccg
     1681 cactggcaga agcctttaat aatggtgagc gcgaaaccct gccccgtgtc attggtgggc
     1741 gctatggtct ttcatccaaa gaatttggcc cggactgtgt actggcggta tttgccgagc
     1801 tcaacgcggc taaacctaaa gcacgcttta cggttggtat ttacgatgat gtgaccaatc
     1861 tgtcactgcc gttgccggaa aacactctgc caaactcggc gaaacttgaa gccttgtttt
     1921 atggccttgg tagtgatggc agcgtttccg caaccaaaaa caatatcaag attatcggta
     1981 attccacgcc gtggtacgca cagggctatt ttgtttacga ctccaaaaaa gcgggcggcc
     2041 tgacggtttc tcaccttcgc gtgagcgaac agccgattcg ttccgcttat ctcatttccc
     2101 aggctgattt tgttggctgc caccagttgc agtttatcga taaatatcag atggctgagc
     2161 gtttaaaacc tagcggcatt ttcctgctta atacgccgta cagcgcagat gaagtgtggt
     2221 cgcgcttgcc gcaggaagtt caggccgtgt taaaccagaa aaaagcgcgc ttctatgtga
     2281 ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc
     2341 agatggcttt tttccatctg acgcaaattc tgcctggcga tagcgccctc gcagaattgc
     2401 agggtgcgat tgccaaaagt tacagtagca aaggtcagga tctggtggag cgcaactggc
     2461 aggctctggc gctggcgcgt gaatccgtag aagaagttcc gttgcaaccg gtaaatccgc
     2521 acagcgccaa tcgaccgcca gtggtttccg atgccgcccc tgatttcgtg aaaaccgtaa
     2581 ccgcggcgat gctcgccggg cttggtgatg ccctccccgt ttcggcgctg ccgccagacg
     2641 gcacctggcc gatgggcacc actcgctggg aaaaacgcaa tatcgccgaa gagatcccca
     2701 tctggaaaga ggaactctgt acccaatgta accactgcgt cgccgcttgc ccacactcag
     2761 ctattcgcgc aaaagtggta ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt
     2821 cgctagatgt gaaatcgcgt gatatgcgcg ggcagaaata tgtcttgcag gtggcaccgg
     2881 aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc
     2941 cagagattaa agccatcaat atgatgtctc gccaggaaca tgtcgaagaa gagaaaatca
     3001 attacgattt cttcctcaac ctgccagaaa tcgaccgtag caaactggaa cgtattgata
     3061 ttcgtacatc gcagctgatt acaccgctgt ttgaatattc aggtgcttgc cccggttgtg
     3121 gcgagacgcc gtatattaaa ttgctgactc agctctatgg tgaccggatg ttgatcgcta
     3181 acgccaccgg ctgttcttca atttatggcg gtaacctgcc ctctacaccg tataccaccg
     3241 atgccaacgg tcgtgggccg gcatgggcga actctctatt tgaagataat gccgaatttg
     3301 gccttggttt ccgcctgacg gtcgatcaac accgtgtccg cgtgctgcgt ctgctggatc
     3361 aatttgccga taaaatcccg gcggaattac tgacggcgtt gaaatcagac gctacgccag
     3421 aggttcgtcg agaacaggtc gcagctttac gccagcaact caacgatgtt gccgaagcac
     3481 atgaactgct gcgtgacgca gatgcactgg tggaaaaatc aatctggctg attggtggcg
     3541 acggctgggc ttacgatatc ggctttggcg gtctggatca tgtattgagt ttgacggaaa
     3601 acgtcaacat tctggtgctg gatacgcaat gctattccaa caccggtggt caggcgtcga
     3661 aagcgacacc gctgggtgca gtaactaagt tcggcgagca cggcaaacgt aaagcgcgta
     3721 aagatcttgg cgtcagtatg atgatgtacg gtcatgttta tgtggcgcag atttctctcg
     3781 gcgcgcagct gaaccagacg gtgaaagcga ttcaggaagc ggaagcgtat ccgggaccat
     3841 cgctgatcat tgcttatagc ccgtgtgaag agcatggtta cgatctggct ctcagccacg
     3901 accagatgcg ccaactcact gctaccggct tctggccgct atatcgcttt gatccgcgtc
     3961 gtgccgatga aggcaaactg ccgctggcgc tggattcacg cccgccgtca gaagcactgg
     4021 aagaaacgtt acttcacgag cagcgtttcc gtcgtctgaa ttcgcagcag ccagaagtgg
     4081 cagaacagtt atggaaagat gctgcagcta atttgcaaaa acgctatgac ttcctagcac
     4141 aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat gctctggata aggattatcc
     4201 aattctaaaa aaaaagcccg gacgactgtt cgggcttgtc tttttatatg ttggaaaatc
     4261 agtggcaatg caatggccca acagaaaata aaatctgtga caataaaggc atataacccg
     4321 cgcagaataa cgtataggaa attaaaagta taatttttat tttgtatgta ttatcttcga
     4381 tacattcacc agacttatta tcatttcaga ggaattattt gattaaggtt ttacttaagg
     4441 cgtaacaaat gatatttatc ggctaactga acttctcctt taggatgttt tcactccccg
     4501 aaatggggat aaaaggcaaa taaaataact aaaggattta ttcaatgaaa agaaaagtac
     4561 tggcactttt aattcctgcc ctgctcgccg caggtgctgc acatgcagcc gaagtttata
     4621 ataaagacgg caacaaatta gatctgtatg gcaaagttga tggcctgcat tatttttctg
     4681 ataattcagc gaaagatggc gaccagagct atgcgcgtct gggttttaaa ggcgaaaccc
     4741 aaattaacga tcaacttact ggctacggtc aatgggaata caatattcag gcaaacaaca
     4801 ctgaatcttc aaaaaaccag tcatggaccc gtctggcatt tgccggactg aaatttgcag
     4861 attacggttc tttcgattac ggacgtaatt atggcgtaat gtacgacatc gaaggctgga
     4921 ccgatatgct gcctgaattt ggcggtgact cttataccaa tgcagacaac tttatgactg
     4981 gtcgagccaa tggcgtcgcg acttatcgta atactgattt cttcggtctg gtaaatggtc
     5041 tgaacttcgc ggtgcagtat caaggtaaca acgaaggagc cagtaatggt caggaaggca
     5101 ccaataacga acgcgatgtt cgtcatgaaa acggtgacgg ctggggtctt tccacaacat
     5161 atgatttagg catgggcttt agcgctggtg cggcatacac ctcttctgac cgcaccaatg
     5221 accaggttaa ccatactgcg gcgggtggtg ataaagcaga cgcgtggact gctgggctaa
     5281 aatacgatgc taacaatatt tacctaggaa ccatgtattc agaaacgcgt aatatgaccc
     5341 cgtttggcga cagcgattat gcagttgcga acaaaaccca gaactttgaa gttactagta
     5401 ccagtttgat tttggtctgc gtccggcagt ctctttcctg atgtctaaag gccgtgacct
     5461 tcacgctgct ggtggtgcag acaacccggc aggtgttgat gataaagatc tggttaaata
     5521 cgccgatgtt ggcgcgactt actatttcaa taaaaacatg tccacctatg ttgactataa
     5581 aatcaacctg ttggatgaag atgacagttt ctacgctgcc aacggcatct ctaccgatga
     5641 tattgtcgct ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg ttatggcggg
     5701 ctttttctgc ttattcttcc tcctttgatc taaattaaaa atgtgaactc cgtcattaca
     5761 caaaaagtgt catctggcgt tacactttat gcggatacta aaacaggagg ttttatgaac
     5821 agaacgattc ttgtccctat cgatatttca gattcagaat taactcaacg cgtgattagc
     5881 cacgttgagg cagaggcaaa gattgatgat gcagaggttc atttcctgac ggtaatacct
     5941 tcactgccct actatgcctc tctgggttta gcgtattccg cagaattacc ggcaatggat
     6001 gacctgaaag cggaagccaa atcgcaactg gaagagatca ttaaaaaatt taaactgcca
     6061 accgacagag tgcatgtcca tgttgaggaa ggctcgccca aagaccgcat tctggaattg
     6121 gcgaagaaga tccccgctca tatgatcatc attgcttccc atcgaccgga tatcaccact
     6181 tatctgctcg gttccaacgc cgcagctgta gtgcgtcacg cagagtgctc cgtgctggtt
     6241 gtgcgctgac actaacgccc gcacattgct gcgggctttt tgattcattt cgcaaatgtg
     6301 ctgacatttt cccctctaat ccgtaccata cacgccacag tttttatatc agatttctta
     6361 tgctgagcgt tccggcatga cgcatactct tctgatgcca tataacgaat tgagtcgctt
     6421 ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga acaaattaca
     6481 aaaacgtctg cgtcgtaacg tgggcgaagc cattgctgac ttcaatatga ttgaagaagg
     6541 cgatcgcatc atggtttgcc tctccggggg taaagacagc tataccatgc tggagattct
     6601 gcgcaatttg cagcaaagcg cgccaatcaa tttttcgctg gtggctgtta acctcgatca
     6661 aaagcaaccg ggcttcccgg aacacgttct gcccgagtat cttgaaaagc tgggcgttga
     6721 gtacaagatt gttgaagaga acacttacgg tatcgtgaaa gagaagattc cagagggcaa
     6781 aaccacttgc tcactgtgtt ctcggctacg tcgcggtatc ctttatcgca ccgcaacgga
     6841 actggggacg acgaagatcg cgttgggtca ccatcgtgac gatatcctgc aaacgttgtt
     6901 cttaaatatg ttctacggcg gcaagatgaa aggtatgcct ccgaaactga tgagcgatga
     6961 tggcaaacat atcgttattc gtccactggc ctactgccgc gagaaagata ttcagcgatt
     7021 tgccgatgca aaagcgttcc cgattattcc gtgcaacctg tgcggttcac agcctaacct
     7081 gcaacgtcag gtgattgctg acatgttgcg tgactgggat aaacgttatc cagggcgtat
     7141 cgagacgatg ttcagcgcga tgcagaatgt ggtgccgtcg catctgtgcg ataccaacct
     7201 gttcgatttc aaaggcatta cccacggttc tgaagtggtt aacgggggtg atctggcgtt
     7261 tgatcgcgaa gagatcccac tacaaccggc gggctggcag ccagaagaag atgaaaatca
     7321 gttggatgag ttacggctga atgtggttga agtgaaataa ccaggatagc gcccgatgcg
     7381 caagcgtatc gggctactct tatggaggcc ggataagacg cggccagcgt cgcatccggc
     7441 aatcccgaat aagatgttta ctcttgcacc cggcaattca acatttcatt attttaataa
     7501 ccgcacccgg cacgtttttc ctttaatctt cccgccctgt aactgtttcc atgctttatg
     7561 agcaacagcc tgacggaccg cgacatagac atgcgccgga tgcacggcga ttttgccaat
     7621 atctgcgcca tcaagcccga tatctcctgt cagtgcacct aatacatcac ccgggcgcat
     7681 tttggctttt ttcccgccat cgatacacaa cgttgccatt tctgcttcca gcgtcacaat
     7741 ggaactatta gctggcggcg tttgccagtt aagttttatc tgcaacatgt cagaaatgat
     7801 attggcccgc tgtgcttctt ccggagcaca gaaactgatc gccagaccgc tatttcctgc
     7861 acgagctgta cgaccgatgc gatgtacatg aacttcaggg tcccacgcca gctcaaagtt
     7921 caccaccagc tcaagcgatt taatatccag accacgcgca gcaacatcag tcgcaaccag
     7981 tacacgggcg ctaccgttag caaaacgtac cagggtctga tcgcgttgct ccagatcgcc
     8041 gtgtaacgac aatgcacttt gctctacttc attcagcgcg tcgcagacag cctggcaatc
     8101 ttttttggta ttgcaaaaca ccacacaaga ggatggctga tgcaagctta ataaccgttg
     8161 caacagagga attttgcctt tactggatgt ctcataaaat tgttgttcaa tgggtggcaa
     8221 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattgc
     8281 ggcgatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     8341 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     8401 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     8461 acgacccggc gttgccacga taatatgcgg cgcatgttgc agcgaatcac gctgcatacc
     8521 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     8581 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     8641 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     8701 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     8761 ggcggcctgc accggcgtca tggttaaata acccaactca ttaaggttcg tgagttgggc
     8821 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     8881 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     8941 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     9001 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     9061 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     9121 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     9181 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     9241 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     9301 tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca
     9361 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     9421 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     9481 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     9541 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     9601 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     9661 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     9721 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     9781 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     9841 actggaatga tcggtcaacg catcgcacac atccaccagc catcccccgc aatcggtcgg
     9901 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     9961 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
    10021 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgaaacggct
    10081 gactcggggc cgtgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
    10141 tgttgtcgcc aaccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
    10201 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
    10261 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt
    10321 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
    10381 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
    10441 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
    10501 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
    10561 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
    10621 ggattgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
    10681 tgcaagacct cacgtttagt cagctacgaa ccggacccta ttccgtctcc tcacaaaaag
    10741 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
    10801 ttcagcgtaa agaagaggaa acagcattaa gctgtcggct tgttttgcga gagctgacag
    10861 aaacagaacc ggtgattatt ttcgaaggta tcgaagcgcc ggcaacgctg ggtttaaaag
    10921 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
    10981 ctaactctgc accaatgttg ttgattgacc cgtcacgaga tggacaaatc gtcgatgcta
    11041 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
    11101 aggaaataaa tatgcttggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
    11161 ccggcggtca taaccctttg aattttgttc ataaactggc ggatggatct actcgtcatg
    11221 tgcagaccta tgccggaccg attgaaattt atggcgacaa gctcatgtta tgtattgtgc
    11281 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgct caccatgacg
    11341 cgatgaccgg attactgaat cggcgacagt tttatcacat tacggaacca ggccaaatgc
    11401 agcatctcgc catcgctcag gattacagct tgttgctcat cgacaccgat cgttttaaac
    11461 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgcc
    11521 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
    11581 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
    11641 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
    11701 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
    11761 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
    11821 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
    11881 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgcg gtgcaatatg
    11941 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
    12001 gtgatttatc ttcccgccct ttaccatgaa taatcagcac attacgcaaa ccatccgcca
    12061 gggcttgttg aatgaaactg aacaccattt tgcggcactc ttccaccggc tggcgcaaaa
    12121 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgct
    12181 gcaacccttc ctgccgaaac tccagcggct gacttagcgg gatgatgtcg agaaatccgg
    12241 tagtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
    12301 ttggatgcca gtggacatct gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
    12361 gaaacagcga tttgtcgtca aggttcatgt aacctcctat gcaccttcac acgtcgtgat
    12421 tcagacgggt taattgttca tacttactgc atcgttccct tcacagattg aaacaatact
    12481 ctacttcata cctgtgagta tctgcgcagg cggggcattt tatactagcc tgttagcagt
    12541 atcgaacagt ttagcccgag tttaattcag cacaggctcc gatcaggatg atttattcat
    12601 agcaggaacc aacaaaaatg cgataaataa cagaccactg attagcagaa aggtaccgaa
    12661 aatgccatag tttgcgcctc cctcagcatc aattgactca ataatttggt tgaagccagt
    12721 atttccgatg tctggtcact tctactgcga ttaaaaaata aagcacttct ggaaatgaag
    12781 tcattcaccg tatggggaac agaaaccatt ttttcaccca ttctcgaaaa atgaaaatta
    12841 gttcgattat tgagaataga gccacacagg gcaataccaa tcgcgccccc aagattacgt
    12901 gttaaattaa atacaccaga ggctaacttc agccgttctt ttggaattcc ccccagcgtt
    12961 aatgttacga tggaggccat cgcaaactgc tgcgaaatcc ctctaattgc ctgtggaaac
    13021 agtaattcct gccatcccca ttcgtgggtt ataggggtaa aaagatacat tgaaaacaca
    13081 aatccgccca gccccgccat caacagccat cgcagattta tttttttact taaccagaag
    13141 taaaagggaa cagaaaataa ctggaaaata cctgttgtgc aaacggcaaa acctatctct
    13201 tcggcattta atccgcgaat ctgccccaaa aacacaggta ttaaatatac agtagagaat
    13261 atcccaaccc ctccagaaaa agaaaaaaaa catcccagag tgaaattttt atccttaaaa
    13321 gcatgaagat ccattatcgg attggatatt gtaagtgtac gcgcagcaaa taaaataaaa
    13381 ctcaccaatg ccagaactga tgtcagcaaa atagtattat catccagcca tccccagcgc
    13441 gctccttctt caagggtata ttccagacat cccagagtca tggccaacag gataatactc
    13501 ggataatcag caactttcag taaagagaga tcaggtttat caaaattaac cagaaaagga
    13561 atactcaaaa ctaaatatat gccaggtaat atattgatat aaaatagcca tcgccagtcg
    13621 aggttatcag tgagccagcc ccccagagtt ggccctagcg tcggtgacag tgaggccagc
    13681 gcactgacta ccgcagcagc caaccccaac tctttaccct ggtaataaat aaacgccatc
    13741 gtgaaaacta aagggatcat ggatgcgcca gcagctccct gtaaagcacg aaacaaaatc
    13801 atgatctgaa tattccatgc aagtccacac gcgatactca ttaaagtaaa tattcccgcg
    13861 gatagagtaa aaagccaacg agtggaaaat acccgtgaca gccacccaga gagcggaata
    13921 acaataatct cagcaattaa atagctggtc tgaagccagg ccatttcgtc tttacctgcg
    13981 gacagaccac cacctatttc atttagggat gacgatacaa tttgaatatc aagctgagca
    14041 ataaataatc ccatacacat actggcgaaa gcaatatgtt tacgcataga catcatctct
    14101 catcaacact gacggtgacg gacagtcccg ggcgaagaag ttgctttaat tcctttgcgt
    14161 cgtcaaatgc aatccgtacc gggacacgct gaacaatttt ggtgaaattg ccagtggcat
    14221 tttctatcgg aatcaagctg aaactggctc ctgtcgctgg ggaaaggctt tcaatgtgac
    14281 cgtgaaacac ttcccctttg agaatatcag cacggatctc agccttcatt cctggcttca
    14341 ttcccgctat ttggttttct ttataatttg catcaaccca caactcactg actggtacta
    14401 gtgaaactaa cgatgtgccg ccttccaccc aactgcctgt atgggcgctg cggtttgcga
    14461 caatgccatc aatcggcgca
//
LOCUS       DAOFMX010000094        14452 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.094, whole genome shotgun sequence.
ACCESSION   DAOFMX010000094 DAOFMX010000000
VERSION     DAOFMX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.094"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(337..582)
                     /locus_tag="QBA20_003407"
     CDS             complement(337..582)
                     /locus_tag="QBA20_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293825.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGINVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="QBA20_003408"
     tRNA            complement(1227..1302)
                     /locus_tag="QBA20_003408"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="QBA20_003409"
     CDS             complement(1408..2115)
                     /locus_tag="QBA20_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDQ6293826.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="QBA20_003410"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="QBA20_003410"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDQ6293827.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="QBA20_003411"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="QBA20_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDQ6293828.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="QBA20_003412"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="QBA20_003412"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDQ6293829.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="QBA20_003413"
     CDS             complement(7299..7574)
                     /locus_tag="QBA20_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDQ6293830.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="QBA20_003414"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="QBA20_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDQ6293831.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="QBA20_003415"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="QBA20_003415"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDQ6293832.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="QBA20_003416"
     CDS             9534..9860
                     /locus_tag="QBA20_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDQ6293833.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="QBA20_003417"
     CDS             10044..10763
                     /locus_tag="QBA20_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDQ6293834.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="QBA20_003418"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="QBA20_003418"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDQ6293835.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13064
                     /locus_tag="QBA20_003419"
     CDS             12102..13064
                     /locus_tag="QBA20_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDQ6293836.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(13165..>14452)
                     /locus_tag="QBA20_003420"
     CDS             complement(13165..>14452)
                     /locus_tag="QBA20_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDQ6293837.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILESVVTT
                     SIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMT
                     PAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQH
                     LIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3790 a         3466 c         3713 g         3483 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg ataccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc ataccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt gatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
    13021 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctagaaaaaa gataccgaac
    13081 aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata
    13141 aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga
    13201 tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatagcag
    13261 caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc
    13321 actacccacc ggcggtgtta acagcccaat acaaaggttg gccaccatca taataccgaa
    13381 atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag
    13441 tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag
    13501 caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat
    13561 atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat
    13621 cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg
    13681 gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc
    13741 ggtaaaaatg ccgcccatca caatgaacac cagaagcaaa cttggtaagg catccagaaa
    13801 cgctttacag gcctgagcaa aggttgctct ttctgaaagc gggtagccac ggcgtttagc
    13861 aataatgccg cagactatca ttaccgcaag acccattaag atccctggca aatatcccgc
    13921 cataaacagc gatgctacag aaacgccgcc agctgtcagg gaaaacacaa ttaacacgtt
    13981 tgagggcggt atcagtaatc cagtaataca agatgagacg tttacagcgg tagaaaatgc
    14041 cggatcataa ccttctttgg tctgaatggg attcagtgtg ccaccaacgg cagcagctgc
    14101 cgcaacggct gaaccagaaa tggaaccaaa catcatattc gccaggacgt tcacatgacc
    14161 aagcgaacca ggaacgcggc caaccattac ctgggcgagg ttaatcaaac gtatcgcaat
    14221 accgccgctg ttcatcagtg ttccggcaaa gatgaaaaag gggattgcca acagggcaaa
    14281 gttatccagt ccattagcca gtcgttgcga aacagtgatt gccgcgatat cccagggaaa
    14341 catcaaaagc attgaagcta cagtggcaat accgattgag aacgaaatgg gcacaccaat
    14401 aaagaccagc acaaaaaaac agccaaacag tgtaatcgcg atataactat cc
//
LOCUS       DAOFMX010000095        14318 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.095, whole genome shotgun sequence.
ACCESSION   DAOFMX010000095 DAOFMX010000000
VERSION     DAOFMX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.095"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..3051
                     /locus_tag="QBA20_003421"
                     /pseudo
     CDS             1..3051
                     /locus_tag="QBA20_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="QBA20_003422"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="QBA20_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="QBA20_003423"
     CDS             6768..7175
                     /locus_tag="QBA20_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293838.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="QBA20_003424"
     CDS             7717..8805
                     /locus_tag="QBA20_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDQ6293839.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="QBA20_003425"
     CDS             8816..11095
                     /locus_tag="QBA20_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293840.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12224
                     /locus_tag="QBA20_003426"
     CDS             11088..12224
                     /locus_tag="QBA20_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDQ6293841.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYAIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            12221..14224
                     /locus_tag="QBA20_003427"
     CDS             12221..14224
                     /locus_tag="QBA20_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293842.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3548 a         3756 c         3654 g         3360 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 gtctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacttggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatga tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgccgcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggtcc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactccagga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacgccat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg acacaagcgt cgttgatctc acggcagacg
    11881 ttgccgatcc ggtagagtta ttaatgaaag tgcaattagg cggcggaacc aatatcgcca
    11941 gtgccatgga gtatggtcgg caacttattg aacaacctgc aaaaagcgtc attatcctcg
    12001 tgagcgattt ttatgaaggg ggttcatcat cattgctgac gcaccaagtg aaaaagtgtg
    12061 tccagagcgg catcaaagtg ctgggactgg cagcgctcga tagcacagca acaccttgct
    12121 atgaccgcga tacggcccag gcgctgatta atgtcggcgc acaaatagcc gccatgacac
    12181 cgggcgagct ggcatcatgg cttgcggaga atcttcagtc atgaattcac tacgtccgga
    12241 attattagaa ctgacaccgc aggcactgac ggcgttaagc aatgccggtt ttgttaagcg
    12301 cagccttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga acagcgcttt
    12361 aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacggccagg cactgaaaga
    12421 ggctcagtgc acctatgggg ccagcggcat gtgccgtcat cgcgtgatgc tggtgttaag
    12481 ttatcaacga ctttgtgcta ccgcccagcc tacgggaaaa gaagaggaat gggatccggc
    12541 aatctggctg gaagaactgg ctacccttcc cgatgccacc cgcaaacgcg cacaggcgct
    12601 ggtcgctaaa agcatcacca ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg
    12661 cttaccgatg agcgatgtgc gtttttattc ccgcagcagt attcgtttcg cccgttgtga
    12721 ttgtattgaa ggcacacttt gcgaacatgt cgtactggca gtacaggcct ttgtccaggc
    12781 aaaagcgcag caagcggaat tgactcattt aatctggcag atgcgcagcg aacacgtcac
    12841 atcatctgac gatccgtttg ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca
    12901 actaagccag gcattatggc tgagcggcat cagccagccg cttattcact acgaggccgc
    12961 tttcagccgc gcgcagcaag cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct
    13021 acggcaactg cgcgccagcg ttgatgcctt ccacgcccgc gccagccact atcatgccgg
    13081 agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc
    13141 ccggagcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg
    13201 cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg
    13261 gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat
    13321 tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa gactcaccgg cagctacgcg
    13381 tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc
    13441 aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt
    13501 accgctgtcg cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt
    13561 ggctttgcgg gaatatttac gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt
    13621 cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg
    13681 ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctgc tgacgttatc
    13741 actgccagcc tcagccagcg cgccttatgc cattgaacgc atggcggcgc ttttgcaaca
    13801 aacagacgac cccgtgtgtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac
    13861 actggaacca caggtaatga tgacaaagac tcgtgcctgg gcgctggacg cagaaactgc
    13921 gcctgtggtg gtatcactac cttctgccag cgttctgcct gtgccgtcta ctgctcatca
    13981 gttgctgatg cgctgccagg cgttacttat tcaactgctc cataacggct ggcgctatca
    14041 ggaacagagc gctattaatc aggcagagtt gctggcgaat gacctcaccg cggtgggttt
    14101 ttatcggctg gcacatgtgt tggcacaatt tcgtaataca gaaagcgagg cacgggtaga
    14161 agcaatgaat aacggtgttt tgctttgcga acaattattc cccatgcttc agcaacaagg
    14221 atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta
    14281 ttaggcatat cctatatagc ccctggtgag agtcacca
//
LOCUS       DAOFMX010000096        14118 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.096, whole genome shotgun sequence.
ACCESSION   DAOFMX010000096 DAOFMX010000000
VERSION     DAOFMX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.096"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..126
                     /locus_tag="QBA20_003428"
     CDS             <1..126
                     /locus_tag="QBA20_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HDQ6293843.1"
                     /translation="CENFGIRNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(188..910)
                     /locus_tag="QBA20_003429"
     CDS             complement(188..910)
                     /locus_tag="QBA20_003429"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDQ6293844.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(912..2083)
                     /locus_tag="QBA20_003430"
                     /pseudo
     CDS             complement(912..2083)
                     /locus_tag="QBA20_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2110..3726)
                     /gene="bglH"
                     /locus_tag="QBA20_003431"
     CDS             complement(2110..3726)
                     /gene="bglH"
                     /locus_tag="QBA20_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HDQ6293845.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(3812..5206)
                     /gene="bglB"
                     /locus_tag="QBA20_003432"
     CDS             complement(3812..5206)
                     /gene="bglB"
                     /locus_tag="QBA20_003432"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HDQ6293846.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5225..7102)
                     /gene="bglF"
                     /locus_tag="QBA20_003433"
     CDS             complement(5225..7102)
                     /gene="bglF"
                     /locus_tag="QBA20_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HDQ6293847.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSVWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7236..8057)
                     /gene="bglG"
                     /locus_tag="QBA20_003434"
     CDS             complement(7236..8057)
                     /gene="bglG"
                     /locus_tag="QBA20_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HDQ6293848.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8344..9069)
                     /gene="phoU"
                     /locus_tag="QBA20_003435"
     CDS             complement(8344..9069)
                     /gene="phoU"
                     /locus_tag="QBA20_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDQ6293849.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9084..9857)
                     /gene="pstB"
                     /locus_tag="QBA20_003436"
     CDS             complement(9084..9857)
                     /gene="pstB"
                     /locus_tag="QBA20_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDQ6293850.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(9949..10839)
                     /gene="pstA"
                     /locus_tag="QBA20_003437"
     CDS             complement(9949..10839)
                     /gene="pstA"
                     /locus_tag="QBA20_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDQ6293851.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(10839..11798)
                     /gene="pstC"
                     /locus_tag="QBA20_003438"
     CDS             complement(10839..11798)
                     /gene="pstC"
                     /locus_tag="QBA20_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDQ6293852.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(11885..12925)
                     /gene="pstS"
                     /locus_tag="QBA20_003439"
     CDS             complement(11885..12925)
                     /gene="pstS"
                     /locus_tag="QBA20_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDQ6293853.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13173..>14118)
                     /locus_tag="QBA20_003440"
     CDS             complement(13173..>14118)
                     /locus_tag="QBA20_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6293854.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3634 a         3639 c         3198 g         3647 t
ORIGIN      
        1 tgtgagaatt tcggtattag aaattctgat ctagctaaat gcctgcaaaa gggtggtgat
       61 tgtcagggtt atctcttccg ctgtctccac tgcggcaagc tgagactgtg gggtgatttt
      121 tcgtagttat ttaaataatg agaacaggcc ggagcgtaat tcatacatcc ggccttattt
      181 cttcagctta accgaacgct aattctgcgg cagcggcttt atcagcgatt accatcagcg
      241 aaggatgcag ctgcaacacg gaagctggaa catcttcggt aaccggacct tgcagcacat
      301 ttttcagtgc ctgcgcttta cctgcgccac tgacaattat cagcaagttt ttcgccgcca
      361 tgatgctttt cggccccatc gtcacgtagc tgtcaggcac taacgaaaag tcgccgccca
      421 actcaccatg cgccacaata tcaaccatct ctccctggat cgggaactcc acggtttgct
      481 catggaaatg ggtcgtattc ggcaaattgc cacaaaaatg accatctgca cctaatccca
      541 gcaccaccaa atccaggccg ccttcacgcg ccagtttctg atcatgctcg cggtagttat
      601 caatagtgag cttctggata ttctcttctt tgatccccgc aggggtgaaa aacagattac
      661 gcagattggt aatcgttacg ccttctccct ctttgccgcg aaagggaatt tcatcgaaat
      721 tatagaaata gcagttatcg taccaaggct tacctttaac cagggtggtg aggtattcat
      781 acatgccttt gggcgtgcta ccggcggtaa ttgccaggtt aacacgacgc gtcttcgaca
      841 tataaccaag cagatggtgt gccgcgacac ggctcatttc ctggtaatct tcggtaatga
      901 ttaatttcat cttaatgcct tattttaaat ttctgtacga aggctgcata ggcaggccgc
      961 cagacatcca tttcatggtt caggccggga tattcctggt aatcaaactt aatttttttc
     1021 tgctcaagct cagttttcag cccggcgata tccttgccgg ttacgacatc tttatcacca
     1081 acgaccacag taaaattacg tagttgctgg ttaatagctg ccggatcgtt cagccgggcc
     1141 gcgacacctt catccggtac ggttgtcgtg gtaacaccac tgaatgtggc cagccaaagc
     1201 tttccagatg attcattccg gaaaccagcg cctggtaccc gccttgtgaa agccctgcca
     1261 gcgcgcgacc atcggcatct ttacggacac taaaacgctt gctaatcagc gggataatat
     1321 cgttcatcag ttcgcgatct gccgctttag cattcagcgg ataaaagact ttacgtcttt
     1381 cctgaggcac gaaatcttcg ggaataatgc ccttcgcatc ggtttcggta tccgggatca
     1441 ccaccagcat cggtttaatt ttcccttcag ccagcaggtt atccatgatt tgcgggatac
     1501 gcccctgatc gatagcggaa cgtccggtat caccaaaacc gtgatagaaa tagagcactg
     1561 gcaaaggctc gcccatgccg gtgtatcctg gcggggtcca gacatacatc tgacgttcag
     1621 attgcaatgc gttggagtgg taagttatgg cgatcaaatc accatgcgca acagaacgcg
     1681 tatccagata actgcctggc accagaatca tactggagtt aacctggcgc tgaggcttag
     1741 tcattgcggt gcctgtatca atgctgcgta caccatcaac attgaaaaaa tactcgtaca
     1801 ggttgccttt caggataggt gtgcgccacg accagactcc tgcttcgtct ttggtcatcg
     1861 ggtgaatatt gtccggaacc ggaacaccca ccactacaga gacatttttt gccccagggg
     1921 caaagtagcg aaaggtcacg ctgttatcgg cattgacctg agtcacatac tgtttcaccg
     1981 ggatagtggg tgccgggctg gctggcatat ccgctgctat ggcccactga gcagaaatac
     2041 cgctggcaat agccagcgtt aaagcagcaa tttttatatt catcgtaata ttcctttcat
     2101 taagttccat taccaccaga tttcagcctg ggcaccgaca gcaaactggg cgtctttatt
     2161 gtcttcgaag gtgaagccat ccagttcgtt ttccagggct ttgatatacg tggcgtagaa
     2221 acgaatttcc agacgcgagg tcaacatact ggtattgact ttaaaggtat ggaagagcgt
     2281 ggttttataa ccagactcat taaatttatt actattcgca tctttgtttt gctgggtgaa
     2341 atagcccagt tcaacacctg tctggttata ttggtcccaa atataggccg ggcgaacaac
     2401 cgcacgaata gattcgaaat cagagtgtgc gcctgtttcg tagctataaa tattgttacc
     2461 gaaggagtaa acaatcgcgt tagccacaat aaaatgatcg ccgatatagg cttcgccctg
     2521 cgaggtcaga cgaactgctg ttccgccggt gtgatcacca taataacgac cattaaaggt
     2581 ggtaaatgga cttgcgccag cataacggcc aaagttcctg gcgatagagt tattcgcaac
     2641 caggaaggag aattcgttga agccaccttt atcaaatttc tgcgttaaag atgtgccgaa
     2701 catccaggta tctttccagt cataataccc gttattatcc tgattatctt tttccgatgc
     2761 gctttcgttt gccgtgacat aacgaccact caccattaag gtcgctttat cccataacgg
     2821 gatatcttta tagcgtaaat caatagtatg ggtattaatc tgctgtttgt tttgcaggct
     2881 gcgatcgtaa tcatcgacat cttcgcgaac cagcgcgata tcaattttac ccggaccgac
     2941 tttccagttt tccagaccta cacccgctgc agcatcagta cgctgcgttt tccagtccag
     3001 catctggatt tcaattttcg gcgcaccgtg tttacccacc cagaaatcag cctctggcgc
     3061 aaagggcagg aaacctttgg tggtaacgta catatcggag aactgcataa agttctcgcc
     3121 accggcatta tcgccaaacc agccggtaga gtactgctga ccaacgttac catctatcat
     3181 cacaacggca tcaacccgtt tgccgttttc gttgtagaca cgttgtttta actgcaaatc
     3241 aaaccagccg gagtattcgt taccaaagcg gcccagagaa ccaatcgccc atgatttagg
     3301 tgaaccatga gaggcggtcc cccaaccaga acggtaatag ccgttgtaac taaagccaat
     3361 ttcatctttc acaaacttac tgaaatcttt cagagtcatg gaagaataaa tcgagcctgt
     3421 ctcactggca tttttctctt catttttgac cagtacagca tcaggttttg cggcactact
     3481 ggtcgggaac ggattggcgg catacccttg atcattcgta cttacgctac gattcaccgt
     3541 cgctggcgta tattttttct tctcttcatc tttatacttt ttgagttcgc tttgcgtttc
     3601 tcttaacgct ttttctaata actcaaggcg ttgttccacc gttaatgatt cagccaatga
     3661 ttgtgcagaa aaggctaacg gtgccattaa taagatggca gaggtaataa gatttcgtct
     3721 aaacataata tccctttatg gtgcaaagaa agaattaacg tatcgcatcc agactgttct
     3781 gaatgcgacg ataattaagg tgctttattg gttatttttt taatgacagc ccccgcgttt
     3841 tgatcacttc tgcataccat ccgaagcttt tcttacgcgt tcttgtgagg cttccttcgc
     3901 cattatcatc acgatccaca taaataaagc cgtagcgctt agacatttgt gaatgagatg
     3961 cactgactaa atcaattggc ccccaactgg tgtaccccat aatatccaca ccatcggcaa
     4021 tcgcttcatt tacctgtacc aggtgatcgt ttaaataggc aattcgataa tcgtcctgta
     4081 tcgaaccatc cgcttcaacg ctgtctttta cgcctaatcc attctcgaca ataaataacg
     4141 gtttttgata acgatcccaa agcgtattta acagaacccg taatccaacc ggatcaattt
     4201 gccaccccca ctctgaactt ttcagatgcg gattggggat catattcagt atgttgccct
     4261 gcgcattttt attaatgctt tcgtcgtggg aaacacaacc agtcatgtaa taactaaaag
     4321 agatgaaatc tacagtatgt tttaaatctt ctgcgtcact ttcagtcatc tcaatggtga
     4381 tattgtggtc gcggaagaaa cgctgcatat agccgggata ttggccacgc gcctgaacat
     4441 caccaaagaa catccagcgc cggttctctt ccatggcctg caacatatcc tgtggctggc
     4501 aggtgagggg gtaaaccagc ccaccgagaa gcatattgcc gattttcgct tcggggatca
     4561 ggctatgata ggctttaact gcccgcgcac tggcaaccag ttgatgatgg atcgcctgat
     4621 aaacttccgc ctcgccactc tcttctgcca gccccacgcc cgtgaatggc gcgtgtaacg
     4681 acatattgat ttcattaaac gtcagccata acgctacttt atgttggtag cgggtaaaga
     4741 ccgtgcgggc gtaatgctcg aagtgatcga tgaccgcccg attagcccaa ccgccgtagt
     4801 ttttcaccag cccatatagc atttcgtaat gggataacgt taccagcggc ttgatccccg
     4861 cctgcgccat ttcatcaaac agccgatcgt aaaacgctaa ccccgcttca ttcggttcgg
     4921 cttcgtcgcc ctgagggaaa attcgcgccc aggcaatgga aatacgcaga caggtgaagc
     4981 ccatctcggc aaataacgcg atatcttccg ggtaacggta ataaaaatcg atggcgacat
     5041 ctttgatatt ctctttcccc aggatgcgcg gttccatttt tcccattacg ccatgaggct
     5101 gtaaatctga ggtcgtgatc cctttgccat cttccagcca ggcaccttcc acctgattgg
     5161 cagctgttgc gccaccccaa agaaatgttt ctggaaatgc tttcataatt aactcctttt
     5221 atcgttagcg aatgatggat aacagcggtt cacctgcgct tatctgcgcc gtgccgtggg
     5281 gtaatacgtc tgtaaaatca tcgctattac tgattaatac cggcgtcgtc agatcaaatc
     5341 cggcctcgcg aatagcaggg atatcaaaag aaatcagccg atcgcctgta ttgaccttgt
     5401 cacccacgtt gacgtgagcg gaaaagaatt tgccgtccag ttttacggtg tcgataccga
     5461 catgaatcag gatctccaca ccatcatctg actcaatgcc aatggcgtgt aatgtggcga
     5521 acaacgaagc aattcgaccc gcaaccggag aacgcacttc accaaccgag ggcagaatgg
     5581 cgataccttt acccaacagg ccactggcaa acgtggtatc agcgacgtga atgagcggca
     5641 caatctctcc cgtcatcggt gaacagatac cgccctgctc aggtggtgta ataacctctg
     5701 gtgttttctc ttgcggggca ccctgcgctg gctgacgttt actggtgatg aaatgaagca
     5761 tcaacgtacc aacaaatgcg caacctatgg caatgacacc gccaataacg ctggcccaga
     5821 cggtgaaatc aatccccgtt gacgggatgg tctgcatgaa ggtgaaaata cttggcaaac
     5881 caaaggagta aactttcgtt tgcgcgtagc caatgatggt ggcccccaat gctccactga
     5941 tacaggcgat aacaaagggg tacttacgcg gcaggttgac gccatatacc gctggttcgg
     6001 tgataccaaa cagacccgtc aacgccgctg atcccgccac cacttttttc tgcgcatcgc
     6061 gttcgcagag gaagacgccg agcgccgccc cgacctgcgc cataatggcg ggcattaaca
     6121 gcgggatcat ggtgtcgtag cccagcacgg tgaagttatt gatacacagc ggcaccaggc
     6181 ccctgtgcag tccgaacatg acgaagattt gccagaagcc gcccattacc gcgcccgcaa
     6241 atgcaggaac cgcctgataa agccagagat aaccggcggc aatcatttcg cttatccagg
     6301 ttgatagcgg ccccaccagc agaaaggtga cgggtgtgat aaccatcaga catagcaatg
     6361 gtgtgaagaa atttttgatt gccgacggta accacgcatt aagtcggcgt tccagaatgc
     6421 tgcacaacca gacagaaaaa ataatgggaa taaccgatga cgagtaattc aacaatgtga
     6481 ccggaatacc caggaaatcc agccccggcg catccgcttt ttgcccgttc tcgaaggcag
     6541 tcagaattaa tggatgcact aacgctccac caatcaccat ggcagtaaat ggattaccgc
     6601 cgaagcgttt gcccgcggtg tatcccagga ttatcgggaa gaacccaaac aaggcatcac
     6661 tggcgctgaa taaaattaaa taagtaccac tttgttcggt cgtccactga aaagtgagcg
     6721 ccagagccag catacctttc aggatcccgg ttgccgccat cagaccgatc agaggcgtaa
     6781 aaatacctga aataacataa acaaagcggt ttagcagatt acctttctca tcattttccg
     6841 gtgcctgttg cgctttttcg ccaaggcctg ccacgctgtt aaccgccagg aagacatcgg
     6901 ccacatggtt acctatgacc acctgaaact gaccaccgct ttccaccacc ataataatac
     6961 cgggggtctt tttcagtacc tcagcttgcg ctttgctttc atcctttaac ttaaaacgta
     7021 atcgcgttgc gcaatgcatc agactcacaa tgttatctgc gcccccgact cctgcgacta
     7081 tttttctggc taactccgtc ataacttgcc ctctaccgct ttgcggcaaa actccaaaaa
     7141 aaaacctgaa aaaaacggcc tgacgtgaat caagcaattt ttttcaggtt ttgcccgctt
     7201 agtgcggtaa caatccttta ctcagtaata atatttcagt gttctttgcg cacgcgctct
     7261 atatttatgg ctaaaaacat aatctctgcg ggtgaaattt tacgttgata ctgcaaacca
     7321 ataaaaatgg cgatccgttc cgcacattgc catgcttgcg ggtaattttg ttttactgct
     7381 tgttgtaatg attcatcact atcgttaatt gaagcatgtt caagaatacg ccaggataaa
     7441 aacttcagat gtgtaaccag tcgctgataa ctcaagcttt cttcctggta attaaggctg
     7501 aactgaaatt ttattaattg cagcatttcg cgcattaact gcgtgacacc tgcaacatcc
     7561 tccatatttc cgctcatttg ggcactgacc agatgcatgg caataaagcc cacttcatct
     7621 ttcggtaact gcacgcccaa ccgtttatca ataatggtta acgcttcttc ccctagctgg
     7681 aactctttcg ggtaaagccg ctggatatcc cacagcaaca cgttttgctg aaagcgttta
     7741 atcgcaaact ggcaatggtc agttagcgag atataaatac tgtcctgtaa ttttcccaag
     7801 cgctcctgcg ctaaagagat aagacgatca caggttgcca tcacctcaag aggaatatga
     7861 cttaagagtt cgcttaatcg cccgttcagt tcatgactgc tcaaggcata ctctttttct
     7921 attccacttg agttaattct ttcgccagcg cgtttttgaa agccaattcc gcgccccatg
     7981 acgacttttt cccgctgttg atcatcaata accaccacaa cattattgtt gagaattttg
     8041 gtgatttgca tgttcatagc aaggaccttt tttataaaca aaaaaaaacc gacttcacca
     8101 gtattctctg gttatgtcag gttttgcctg cgaatgcagt aacaatccag tcatttatta
     8161 atggttttta taacgaacat ccaggttcgg aaattaattt aattgcgtgc agagaaaata
     8221 gcaatgcgct attgataaaa atatgaccat gctcgcagtt attaactttg tgtaatttta
     8281 ggaatttata aagttatata taacaaatcc caataattaa gttattggga tttgtctggt
     8341 gaattatttg tcgctatctt ttcccgccag cagtttatcc agctcatcac cgcctacatg
     8401 acggaaatcc tgacccttca cgtagtagaa gataaactca caaatattct ggcagcggtc
     8461 gccgatacgt tcgattgaac gcgcacagaa cagcgcggta agtacgctcg gaatagtgcg
     8521 cgaatcttcc atcatgtagg tcatcagttg acgaacaata ccttcgtatt cctgatcgac
     8581 ttttttatct tcacgataaa tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc
     8641 cagcacgtcg tgcagcatct ggatggtatg acggcccagc gactccagac ttaccagcaa
     8701 cggctgatgc tgctgggaga atttctccag cgcagtacgg cagattttgt ccgccacgtc
     8761 gccaatacgc tccagctcgg caatggtttt actgatcacc ataaccagtc gcaggtcgct
     8821 cgccgtcggc tgacgtttgg cgataatgcg cacgcaggct tcatcgatcg ccacttccat
     8881 catgttgacg ttcttgtcgc cttcgatcac gcgcttcgcc agatcgctgt cctggttatg
     8941 catcgcggtg attgcatcag aaagctgctg ctccaccatg ccgcccatgg tcatcacctg
     9001 cgtgcggata ctttccagtt cggcgttgaa ctggccggaa atatgtttat taagattgag
     9061 actgtccata acgcactcct gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt
     9121 ttcttcgctg gcttggtgaa cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg
     9181 tacataaatg ccgtgtggtc ggaacaacgc gcagcctgct gcatgttgtg ggtgacgatc
     9241 accacggtgt aatcctgctt cagttcggtg atcagctctt caatacgccc ggtagagatg
     9301 gggtcgagcg ccgaacacgg ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca
     9361 cgcgcaatac acagacgctg ttgctgacca ccagagagag agtaaccgct ctggtgcaat
     9421 ttatctttgg tttcgttcca caatgcggct ttggtcaatg cccactgcac gcgctcgtcc
     9481 atgtcggcac gggagagctt ctcaaacaga cgaacgccaa aggcgatgtt gtcgtagatg
     9541 gacatcggga acggcgtcgg tttctggaac accatgccca ctttcgcacg cagcagggcg
     9601 atatcctgag agttggtcag gatgttgtcg ccatcaagca gaatttcacc ttccgcacgc
     9661 tgctccgggt acagttcaaa cattttgttg aaggtacgca gcagcgtcga tttaccgcag
     9721 ccggacggcc cgataaacgc cgtaacctgg tttttggcga tatccaagtt gatgtttttc
     9781 agggcatgga atttgccgta gtagaagttc aaattacgaa cctgaatttt actcggggca
     9841 gtttcaacca tactcattgc aatcttttcc tcatcgtacc tgatgctttt cgctaatcag
     9901 gcttaccgta gggccgggta aggcgttcac gccgcatccg gcaaagtgtt aaccgtgttt
     9961 attcttcgca aaaacaacgc gcgccagaat gttcagcagc aggacgcaca gggtaatgat
    10021 caataccccg gcccaggcca gttgttgcca ttccgcaaac gggctcatcg caaacttaaa
    10081 gatcgtcacc ggcaggttgg cgatcggctg catcatgtcc gtgctccaga actggttgga
    10141 gagcgcggta aacagcagcg gcgcggtttc accggcaata cgggcaatcg ccagcaggat
    10201 accggtcata atcccggaca ccgacgcttt cagcgtaatc gcggagatca tcttccactt
    10261 cggtgtaccc agcgcataag ccgcttcacg caggctgtcc ggcaccagtt tcagcatgtt
    10321 ctcagtggtg cggataacga tcggcacctg caacagcgcc agcgcaatta cgcccgccca
    10381 gccggagaag tgctccatct gtgccaccac gatggtgtag acaaacagac caaccacaat
    10441 cgacggcgcg gagagcagaa tgtcgttaat gaagcgaatc acttctgcca gccaggattt
    10501 acgaccatat tccgccagat aaatccccgc cataatgccc agcggcgtac cgaataccgt
    10561 ggcccacaaa attaacagcc cgctacccgc cagggcgttc gccagaccac caccttccgt
    10621 attgggcggc ggcgtcattt cagtgaacag cgccagcgac ataccgtcga taccgcgagt
    10681 gatggtggac attaaaatcc agatcagcca gaacaggccg aaggccatcg tcgccatcga
    10741 gagcgtcagc gcaatacggt ttttgaggcg gcgacgcgcc tgcattttgc ggcgagattc
    10801 agccagcgcc gcagtggttt gcatttcaac catagccatt agcgtgcccc ctcattctta
    10861 gccaggcgca taatcataaa cttcgatgcg gcgaggacga tgaaggtaat cacaaacagg
    10921 atcaggccca gttccatcag tgcggcaacg tgcagaccgg attccgcttc cgcaaattcg
    10981 ttcgccagcg cagaggtgat actattgccc ggcatgtaca gcgaggcgct gtcgagctgg
    11041 taggtgttac cgatgataaa ggtcaccgcc atggtttcac cgagcgcgcg gcccagcccc
    11101 agcatgatgc cgccgataac accatttttg gtgaacggaa gaacgatacg ccagataact
    11161 tcccaggtgg tgcagccaat accgtaggcc gactctttca tcatcaccgg ggtttgttcg
    11221 aacacatcac gcattaccgc cgcaatgtac ggaataatca tgatggcgag gatcacgcct
    11281 gccgcgagga taccgatacc aaatgcgggg ccagagaaca gcgcgccaac aatcgggata
    11341 ttcgacatga tattgccgac cggctcctga aagtaaacgg cgaacagcgg cgcaaagata
    11401 aacaggcccc acatgccgta aacgatactt ggaatggctg ccagcagctc aatggcgata
    11461 cccagcgggc gtttcagcca gccaggcgca agctcagtca ggaacagggc gataccgaaa
    11521 ctcaccggga cggcgatcag cagcgcgata aacgaagtca ccaacgtacc gtagatcggc
    11581 accagcgccc cgtagatatc gttcggtgca tcccactctt tggtccatag gaaagccaga
    11641 ccaaatttct gaatgctcgg ccaggaggag atgatcagag agacaataat gccacccaac
    11701 atcaatagca caatcagcgc cgccagtttt accagcacgc tgaaaattat gtcgcccttt
    11761 ttacccggtg ggttaaaagc gggcttggtt gcagccataa gttactcttc agttaaacgc
    11821 gtttacaaag tcattttgta atgccggatg cggcgtaaaa caccgtaccc ggcctggagt
    11881 tttattagta cagcggctta ccgctactgt ctttaatatt ggtcttccac gcagcgcgaa
    11941 cctgttcaac tacactatcc ggcaggctgg cgtaatccag gtcgttcgcc tgtttagccc
    12001 cggttttgta cgcccagtcg aagaatttca gcacctctgt gccttgttct ggtttcttct
    12061 gatctttgtg gatcagaatg aacgtggtag aggtaatagg ccatgcatct tcgcctttct
    12121 ggttggtcag atcctgagcg aaggttttgc tccagtctgc accttttgct gcattagcga
    12181 agttttcttc ggtcggacta accggtttac catcagcgga gatcagtttg gtgtacgcca
    12241 ggttgttctg cttcgcgtaa gcatattcaa cataaccaat tgcacccggc agacgctgaa
    12301 cgaacgcggc gataccgtcg ttacctttac cgcccagacc aatcggccat tttacggtag
    12361 agccagtacc aacgttgttt ttccactctt cgttcacttt cgccaggtag ctggtgaaga
    12421 cgaaggaagt cccggagcca tctgcgcggc gtactacagc aatgttttgt gaaggcagtt
    12481 tcagacccgg attcagtttg gcgatggctt catcatccca cttcttgatt ttgcccaggt
    12541 agatgtcgcc gagggtttta ccatccagca ccagttcgcc agacttcagc cctggaatgt
    12601 taaccgccag caccacgccg ccaatcacgg tcgggaactg gaacagacct tcctgagcca
    12661 gtttttcgtc agacagcggc gcgtcagagg caccaaaatc aacggtatta gcgataatct
    12721 gttttacgcc accggaagaa ccgataccct ggtagttaac tttattaccg gtttctttct
    12781 ggtaagtgtc agcccatttg gcatacaccg gcgcagggaa ggttgcacct gcacctgtca
    12841 ggcttgcttc tgcaaacaca gagaaagcac tcatcgataa ggtcgcggcg acaacagttg
    12901 cgacggtggt acgcataact ttcataatgt ctcctgggag gattcataaa gcattgtttg
    12961 ttggctacga gaagcaaaat aggacaaaca ggtgacagtt atatataagg aatatgacat
    13021 ttttatgaca gaaaggaatt accaaataac ttactgattt taaatgtgaa gatgaaaatc
    13081 ccacatgcca tctttttgtc atgatgcatt taaaagtgat aaagaaatgg gtgagatgac
    13141 aacgatgtga tgaacaatcg ttgtcagacc tattagcgca caatctcaac agttatcgtc
    13201 gctgttgcag taaattgccc cggggctggg cgagctccag taaggctcgc aggagcagct
    13261 gaaaaagtta cactacctga tttttcacca tacactgttg atttatccat gtctgttggc
    13321 aactcaccac cgttcacttt gatttcctta ttatttttgt cgtaaatttt tatccccacg
    13381 ttagagtttg aagttacgac cattgaattg tccgtattgg gttcggtagc ataaaaagac
    13441 gtactgatat catcggtatt cagtatcccc aggttttcac actcaatagt gactgtttta
    13501 gtaattttat gtgttgttac cgcacttcca acaacagatg aaaaatctgc agctggtatt
    13561 tctttaaaat caaaacaaac tggctttaaa ttatttattt cgcaactttg cggagctgta
    13621 atatcgcccc gaatataaac atcagccatc ggctgtgagt aatccaccgg ggtgtcacgg
    13681 ctaattgttc catacaatgt cactatcttt gtttcaggaa taatcaattt tcctgcaaat
    13741 gtcttcttga tataaaaata tactatcgct ttagccccac tggatatact taaaggttca
    13801 atgcggttgc aattgtactt tgtattacca gacgggttgt tagcatgata ttcaaaaggt
    13861 gcattcacat acccaacccc aagaatatct atagcaatcc caacatcaag gttcggatta
    13921 agatcataat aattaatatt agagtgaacc ccagcataag ttaatgcctg gtttattttt
    13981 gacgtataat agatataggg gaattctcct acgttaccag tatcacaatg acagcgtcca
    14041 agatatgagg atccattagt tacagtggtg ttaaatgtcg ttccaacctt attttcgttc
    14101 gcggtaataa gatatccc
//
LOCUS       DAOFMX010000097        13963 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.097, whole genome shotgun sequence.
ACCESSION   DAOFMX010000097 DAOFMX010000000
VERSION     DAOFMX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.097"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="QBA20_003441"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="QBA20_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDQ6293855.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="QBA20_003442"
     CDS             2219..2767
                     /locus_tag="QBA20_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDQ6293856.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="QBA20_003443"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="QBA20_003443"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDQ6293857.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="QBA20_003444"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="QBA20_003444"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDQ6293858.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="QBA20_003445"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="QBA20_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDQ6293859.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="QBA20_003446"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="QBA20_003446"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDQ6293860.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="QBA20_003447"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="QBA20_003447"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDQ6293861.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="QBA20_003448"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="QBA20_003448"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDQ6293862.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="QBA20_003449"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="QBA20_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDQ6293863.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="QBA20_003450"
     CDS             complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="QBA20_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDQ6293864.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3553 a         3565 c         3524 g         3321 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacaccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcccggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgc
//
LOCUS       DAOFMX010000098        13956 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.098, whole genome shotgun sequence.
ACCESSION   DAOFMX010000098 DAOFMX010000000
VERSION     DAOFMX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.098"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="QBA20_003451"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="QBA20_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDQ6293865.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="QBA20_003452"
     CDS             complement(1604..1777)
                     /locus_tag="QBA20_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDQ6293866.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="QBA20_003453"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="QBA20_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDQ6293867.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..3744
                     /locus_tag="QBA20_003454"
     CDS             3415..3744
                     /locus_tag="QBA20_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDQ6293868.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="QBA20_003455"
     CDS             complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="QBA20_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDQ6293869.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="QBA20_003456"
     CDS             complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="QBA20_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDQ6293870.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="QBA20_003457"
     CDS             complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="QBA20_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDQ6293871.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="QBA20_003458"
     CDS             complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="QBA20_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDQ6293872.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="QBA20_003459"
     CDS             complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="QBA20_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDQ6293873.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="QBA20_003460"
     CDS             complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="QBA20_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDQ6293874.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11245..13920
                     /gene="adhE"
                     /locus_tag="QBA20_003461"
                     /gene_synonym="adhC"
     CDS             11245..13920
                     /gene="adhE"
                     /locus_tag="QBA20_003461"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDQ6293875.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3659 a         3454 c         3247 g         3596 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcgc tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaaagac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacagctgat gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag cggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cttggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgcttg agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtgaaatca acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt
     3721 tgccatatca tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg
     3781 tcagccctta ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac
     3841 tcccctccag aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac
     3901 ggaaaacaca accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga
     3961 tggttttgtt cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg
     4021 tgtaaggatg tagtggattg tggtagacct catcataggt ccccagttct actgcatggc
     4081 cgagatacat caccaacaca cgatcggaaa tgtgttttac cacggccagg tcatgagcaa
     4141 taaaaattaa tgacaatccc atctcacgtt gcagctgctg gagcaggtta accacctgcg
     4201 cctgaattga cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa
     4261 gaataagagc acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat
     4321 agcggttaat caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc
     4381 gaacttcctg gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc
     4441 cgatggtcat acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac
     4501 tgcgaacggc acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa
     4561 catgaccgtc ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc
     4621 atcccgattc ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat
     4681 cgacggcttt gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa
     4741 agtgcacttt aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca
     4801 taacagttcc tccaccggtt taaagcaagc acgcagacgg ccaggcgtaa actcttccag
     4861 cggcggtgcg ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc
     4921 tttcggtaat cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc
     4981 cgcatcaaga cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata
     5041 aaagacatcg cgagcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt
     5101 gtcacagatc cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa
     5161 ttcccgcttc agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc
     5221 tgtagtgggt tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat
     5281 gactcgctga cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc
     5341 ttccggcatt tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct
     5401 catgttctta tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt
     5461 caacgaagtc attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag
     5521 ttcacgctct ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg
     5581 tccgttggca gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga
     5641 ctcacctaca atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc
     5701 cgtgacgtcg ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc
     5761 gtcagcctgt tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact
     5821 ccttaacgat ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa
     5881 aacagtgtca ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc
     5941 gagttcgcgc catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac
     6001 cccaggaagc taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag
     6061 accaccacca caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat
     6121 acaccgccaa cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca
     6181 cgcacaatac gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg
     6241 atgttttggc cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg
     6301 aggatttcca gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc
     6361 gaaccataaa gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt
     6421 gagatacgcc cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca
     6481 aagtagtgac cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca
     6541 taggcaaact gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact
     6601 atcagactgg caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag
     6661 ctgcgccctt cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta
     6721 cttaacatca tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata
     6781 gcacatcgac aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta
     6841 acactaagga atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca
     6901 aaccataaat ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca
     6961 tataggagag cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca
     7021 taggtaaccc tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag
     7081 agccacgggt aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca
     7141 atatcatgaa tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga
     7201 tcgcaaatat catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca
     7261 ttgccagccc catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc
     7321 cagcactaac acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa
     7381 cgggaaaact ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac
     7441 cgaaatcgcc atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat
     7501 taagatgata tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg
     7561 taaaagggct tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata
     7621 gcgtcggaat cgcttccaga caacgacgta gaataaattt taacattgcc ctaccttctg
     7681 gcctgtgcct tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc
     7741 acgtattgcc attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga
     7801 tctttgccgg tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa
     7861 acaggaacaa tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg
     7921 cgctgcgcct cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc
     7981 ttataatgcg cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt
     8041 tcgttgtagt ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg
     8101 tcgaggaacg ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc
     8161 cacaatgaag aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg
     8221 ttgatggtca acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct
     8281 tcgttacgtt tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca
     8341 gtatacggtg gagtataacc ataggcgggc atgtcgccct gcgctttcac tttattaaca
     8401 atgatatcgc ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc
     8461 ggtttctggt tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg
     8521 tccgggatct cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg
     8581 tcgatttcac cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag
     8641 gttacctggt taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg
     8701 attcgttcgt ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca
     8761 ggctgggtcc atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat
     8821 gggtgaacaa gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg
     8881 tgatcatcaa tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca
     8941 tcaataccgg cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga
     9001 tcaacagaac gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac
     9061 catttcgcat ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat
     9121 tcagcgacgc caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg
     9181 tctcggctga tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact
     9241 tctgaaccat tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca
     9301 tcagctgcca gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa
     9361 cttctcttgg tgatgttggt cattgttttt tggactccct cattataatt actggtatta
     9421 accagcatgt gtaatcccgg attgggaccc tgcacaagct cggagacttt ctgctgtcag
     9481 gttactttat cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat
     9541 caccttatgt cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc
     9601 tctttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
     9661 taccaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt
     9721 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
     9781 tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg
     9841 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg cagtggaatt
     9901 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
     9961 tgatggtgag gagaagcagt taaggaaatc cttataacta actgtaattt gagaagaata
    10021 tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
    10081 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
    10141 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
    10201 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
    10261 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
    10321 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
    10381 ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg
    10441 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
    10501 tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt
    10561 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
    10621 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
    10681 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
    10741 cgggaaaatc aaaaagggtc tgaatcacgg ttcgctccga agcaaaagcc ggataatgtt
    10801 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
    10861 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
    10921 atgtagccac caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat
    10981 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
    11041 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
    11101 aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt ttcatcggtt
    11161 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
    11221 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
    11281 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
    11341 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
    11401 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
    11461 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
    11521 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
    11581 accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac
    11641 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
    11701 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
    11761 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
    11821 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta
    11881 ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca
    11941 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
    12001 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
    12061 ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg
    12121 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
    12181 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
    12241 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
    12301 gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
    12361 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct
    12421 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
    12481 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
    12541 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
    12601 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
    12661 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
    12721 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
    12781 ttcttcgaag tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
    12841 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
    12901 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
    12961 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
    13021 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
    13081 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
    13141 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
    13201 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
    13261 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
    13321 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc
    13381 gcatttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag
    13441 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
    13501 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
    13561 cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct
    13621 gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg
    13681 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
    13741 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
    13801 gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact
    13861 gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
    13921 tcagtagcgc tgtctggcaa tataaacggc cccttc
//
LOCUS       DAOFMX010000099        13797 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.099, whole genome shotgun sequence.
ACCESSION   DAOFMX010000099 DAOFMX010000000
VERSION     DAOFMX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.099"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="QBA20_003462"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="QBA20_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDQ6293876.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="QBA20_003463"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="QBA20_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDQ6293877.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="QBA20_003464"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="QBA20_003464"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDQ6293878.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="QBA20_003465"
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="QBA20_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDQ6293879.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="QBA20_003466"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="QBA20_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDQ6293880.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="QBA20_003467"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="QBA20_003467"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDQ6293881.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /gene="wza"
                     /locus_tag="QBA20_003468"
     CDS             complement(6041..7180)
                     /gene="wza"
                     /locus_tag="QBA20_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDQ6293882.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7489..7638
                     /locus_tag="QBA20_003469"
     CDS             7489..7638
                     /locus_tag="QBA20_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293883.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7839..9422
                     /locus_tag="QBA20_003470"
     CDS             7839..9422
                     /locus_tag="QBA20_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDQ6293884.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="QBA20_003471"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="QBA20_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDQ6293885.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="QBA20_003472"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="QBA20_003472"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDQ6293886.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="QBA20_003473"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="QBA20_003473"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDQ6293887.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..>13797
                     /locus_tag="QBA20_003474"
     CDS             13381..>13797
                     /locus_tag="QBA20_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HDQ6293888.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLG"
BASE COUNT         3193 a         3641 c         3493 g         3470 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 acaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagcatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctggcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgagtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgcgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccaacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 atcctgcttt tttccaccag ctcgctgaac atgacctgga cgaccatcaa tattgttgaa
    13681 gccgtggtcg gggcagtgct gctacgtaaa ttgctgccgt ggtataaccc cttgcaaaat
    13741 ctggctgact ggctgcgtct ggcattcggc agcgccattg ttccgcctct gttgggg
//
LOCUS       DAOFMX010000100        13532 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.100, whole genome shotgun sequence.
ACCESSION   DAOFMX010000100 DAOFMX010000000
VERSION     DAOFMX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.100"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..890)
                     /locus_tag="QBA20_003475"
     CDS             complement(<1..890)
                     /locus_tag="QBA20_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6293889.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="QBA20_003476"
     CDS             1131..1931
                     /locus_tag="QBA20_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDQ6293890.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="QBA20_003477"
     CDS             2009..2779
                     /locus_tag="QBA20_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDQ6293891.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="QBA20_003478"
     CDS             complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="QBA20_003478"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDQ6293892.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="QBA20_003479"
     CDS             complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="QBA20_003479"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDQ6293893.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5046..5768
                     /locus_tag="QBA20_003480"
     CDS             5046..5768
                     /locus_tag="QBA20_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDQ6293894.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="QBA20_003481"
     CDS             complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="QBA20_003481"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDQ6293895.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6297..7028
                     /gene="dnaQ"
                     /locus_tag="QBA20_003482"
     CDS             6297..7028
                     /gene="dnaQ"
                     /locus_tag="QBA20_003482"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDQ6293896.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7161..7237
                     /locus_tag="QBA20_003483"
     tRNA            7161..7237
                     /locus_tag="QBA20_003483"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7195..7197,aa:Asp,seq:gtc)
     gene            complement(7368..8414)
                     /locus_tag="QBA20_003484"
     CDS             complement(7368..8414)
                     /locus_tag="QBA20_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HDQ6293897.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="QBA20_003485"
                     /pseudo
     CDS             complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="QBA20_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="QBA20_003486"
     CDS             complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="QBA20_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HDQ6293898.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTPPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="QBA20_003487"
     CDS             complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="QBA20_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HDQ6293899.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11797..12627)
                     /locus_tag="QBA20_003488"
                     /pseudo
     CDS             complement(11797..12627)
                     /locus_tag="QBA20_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12614..13528)
                     /gene="tagK"
                     /locus_tag="QBA20_003489"
                     /pseudo
     CDS             complement(12614..13528)
                     /gene="tagK"
                     /locus_tag="QBA20_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3101 a         3523 c         3612 g         3296 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcgc tactgcctgc accaatcgtc aaactttggc ctggcttcag
     3061 cttagatccg cgcagtttgt tccactgctg caaatctttg gtgcttacgc cgagacgtga
     3121 agcgatgctt gaaagcgtgt cgccagagcg tacggtgtaa acacggctgt taagcggcgt
     3181 attgttggca accagcgtcg actgtacagc agcaatttcg cctgaagcga gagattcacg
     3241 cagttgatct gcatgcttct ttggcaccat cacgtactgc ggcccacttg cgcccagcgt
     3301 ggagcctttc acgccagcgt tgaatgtctt cagcttgctg acggaaatcc ccgccatatc
     3361 tgcaaccttc gccatttcaa ccgggctact caggtgcaca cgcgccagag cacggctttc
     3421 atcggtcgtt ggcagacgta cgccataacg cttgctgttt ttgagaatat cactcaatgc
     3481 cagcatttta ggcacgtact gcttcgtttc ctgcggcaac ggtaacgacc agaagtccgt
     3541 ggatttccca cgcgctttgt tcgttttaat tgccttcatg acccgacctt cgccgctgtt
     3601 ataagccgct acggtcagaa gccagtcgtc gtcaaacatt ttgttcagac gctgcatcat
     3661 attcagcgcg gcagttgttg aagcaacaac atcgcgacgc gcgtcataat tgcgggtctg
     3721 tttcaaacca taattgcgcc ccgtgctcgg aatgatctgc cagatgcctg cggcattggc
     3781 gccagacgtt gcgtgaggat caaaagcgct ctccactatg ggtagtagta ccagttccat
     3841 aggcatgtta cgttttttaa cttgccctgc tatccagtac atatacggct ctgcccgtaa
     3901 agttacatcg tggagatagc tcttattgcg taaatatttc tgtttctgtt cgcgaatccg
     3961 gtcattttcc ggaattccca tctttagctc gtcgccaatg aaagcccaca agtcaccatc
     4021 tggcgcgata gacgtcccat cgtccatcca tcgtgcctga cttgtaaact ttgctgcttc
     4081 cccttgacca gctgcagaaa ggctctgtgc gtgctgttga acgttgccgg tactctggca
     4141 acccacgagc aggacagagg cgagtaatat cgcttttgcc ttcatgtgtg tgtcaatagt
     4201 tgcttaaaag acgaccgatc ataacggcga acggagccga tgacaagaaa gttttatcag
     4261 aacctatctt tctttgacct taaccatgca aaacgctctt caggttgttg caataatgtt
     4321 tcttcattaa ttccattaat taaatcaata tcttccgttc ttaaaaaaac attaatttgc
     4381 cgctcatttt tcagaattac gggtagtgtt atttgatttt ttgcccgtaa ctccttaact
     4441 ttacgataat aatcatttat ggacaaatcg tgcggaaaaa tactcaaagc aaacttcata
     4501 tttgataacg tatattcatg agcacaacat accaatgtat cgtcaggtaa cgcacttagc
     4561 tttttaagtg attgatacat ttgtaaagct gtcccttcaa acaaccgacc acacccacca
     4621 gaaaacagcg tgtcgccgca aaatagataa ggtttactga agtaacagat atgtcctaaa
     4681 gtgtgacccg gtgtggtaat tacactaaat tcatgcccta aaacgaaggc agtttcgcca
     4741 tctttgacta cctgtgttgt tcccttatct tgtgtctctt gtggaccata caccacaatt
     4801 tgcggaaact tttccaccag ttcttttacg ccgccaacgt gatcgtggtg atggtgggtt
     4861 agaaatatgg cctccggttg ccagttattg gcggtaatgg cgtttaatac tggctctgcg
     4921 tctccgggat cgacaatcag gcagcgacct gcctcatcat tcaaaaccca gatgtaattg
     4981 tcatcaaagg cgggaatact gttaagattc atagattacc tctcagtgta aaacggaagg
     5041 ttgtgatgaa accggcaaga gtccctcaaa ctgtcgtggc tcctgattgc tggggcgatt
     5101 tgccctgggg agagctttat cgcaaggcgc tggagcgcca gctcaacccg tggttcacta
     5161 aaatgtatgg ttttcatctg cttaagattg gcaatttaag cgcagaaatc aattgcgaag
     5221 cgtgcgcggt ttctcatcaa gtgaatgttt ctgcgcaagg aatgcccgtc caggtgcagg
     5281 cggatccact tcatcttcct tttgccgata aatccgttga tatttgtcta ctggcacata
     5341 ccttgccgtg gtgcaccgat ccgcatcgtt tattacgtga agccgatcgg gtactgattg
     5401 atgatggctg gctggtgatt agtggcttca accccatcag tttaatggga ttacgcaaac
     5461 ttgtgccggt attgcgcaaa acctcgccct ataatagccg gatgtttact ctgatgcggc
     5521 aactggactg gctctctttg ttgaattttg aagtgctgca cgccagccgt ttccacgttc
     5581 tcccgtggaa caaacacgga ggaaaactat tgaatgcgca tattcctgcg cttggttgct
     5641 tacaacttat tgttgcccgg aaacggacta ttcctttaac gctaaatccg atgaaacaga
     5701 gtaaaaacaa gccacgaatt cgccaggcgg ttggagccac ccggcaatgt cgtaaaccac
     5761 aggcttaaac ttcgacttgg tagcccgtat cttccagtgt gggattcatc gccgcggcac
     5821 gagccagttc atcacagcgt tcgttttccg ggtgtccggc atggccttta acccattccc
     5881 atttgatttg atgctgcccc aatgcagcat caagacgttg ccagagatcg acatttttta
     5941 ctggtttttt gtctgctgtt ttccagccac gttttttcca gttatggatc cactgggtga
     6001 taccctggcg gacatactgg ctgtcggtac tcaaaatgac ttcgcaatgt tcttttaacg
     6061 cctccagcgc gacaatagcg gccatcaact ccatacggtt gttggtggtg cgggtgtagc
     6121 ctgcgctaaa ggttttctcg cgtccgcgat agcgtaaaat agcgccgtaa cccccaggtc
     6181 caggattacc cagacacgaa ccatcggtga aaatttctac ctgtttaagc atctctggta
     6241 gacttcctgt aattgaatcg aactgtaaaa cgacaagtct gacataaatg accgctatga
     6301 gcactgcaat tacacgccag atcgttctcg ataccgaaac caccggtatg aaccagattg
     6361 gtgcacacta tgaaggccac aagatcattg agattggtgc tgttgaagta gtgaaccgtc
     6421 gcctgacggg caataacttc catgtttatc tcaaacccga tcggctggtg gatccggaag
     6481 cctttggcgt acatggtatt gctgatgaat ttttgctcga taagcccacg tttgccgaag
     6541 tagccgatga gtttatggac tatatccgcg gcgcggagtt ggtgatccat aacgcagcgt
     6601 tcgatatagg ctttatggac tacgagtttt cgttgcttaa gcgcgatatt ccgaagacca
     6661 ataccttctg taaggtcacc gatagccttg cggtggcgag gaaaatgttt cccggtaaac
     6721 gcaacagcct cgatgcgcta tgtgcccgct acgaaataga taacagtaaa cgaacgctgc
     6781 acggggcatt actcgatgcc cagatccttg cggaagttta tctggcgatg accggtggtc
     6841 aaacgtcgat ggcttttgcg atggaaggag agacacagca gcaacaaggt gaagcaacaa
     6901 ttcagcgcat tgtacgtcag gcaagtaagt tacgcgttgt ttttgcgaca gatgaagagc
     6961 tggcagcaca tgaagcccgt ctcgatctgg tgcagaagaa aggcggaagc tgcctctggc
     7021 gggcataaat acatgtaaaa ggcgctaaaa atagcgactt gggcgatttt tgcagcaaac
     7081 gattcaaaag atgagaaaaa tcgttgacga aggtcgaggc aatccgtaat attcgcctcg
     7141 ttcccaacgg aacacaacgc ggagcggtag ttcagtcggt tagaatacct gcctgtcacg
     7201 cagggggtcg cgggttcgag tcccgtccgt tccgccaata ttcagaaagc ccgaatcagt
     7261 aatgattcgg gctttcgtcg ttttggtgaa gtgagaattt ttcactccga agtaaaacgg
     7321 atatgattta aaaaatggcc agacattata aattctggcc acttctgtta ctccagcgca
     7381 tcccatttct ccagcagttc tactggaatt tcatttatta cctccgagac gcgttttccg
     7441 accaggcgtt ctgcctggcg cagcaaagga atggtcgggc tgctcggttc gctgtgctca
     7501 aaccagcgac ggatgacacg caggcgttcc agcgcgtcgt ttcggtcgcg gatctgccgg
     7561 ggttctgcgc cggtcatcgg tgatatttcc tccgcgatat gcaccggtag cgcaatctgc
     7621 tctgcctgca tctgttcagt atgaacgatg gtggcgtttt ccggctgctc agcctgtggt
     7681 tgtggcagga tctccggcgt ggtgctctgc actgctcccg gtaacagggc cagcagttgc
     7741 gtcagacggg agaagtccgg tgcctggtca ttcagcgttt cacgcgcatg gcgttgcagc
     7801 cgctcagtgg tctctgctgc ctggcgaaag gcatccagcg gcagtgtgcc gcgcgcttcc
     7861 aggtctgcca gttgctggcg taccgattct ggtgccagcg catccgccgg gcgtaatgca
     7921 gatagcgaac gctccacatc ccgcacctga aggcgcatgg cagcattatt tgacagtgta
     7981 atgccacgaa tatccgccat tacaccttcg tggtccagca acgccgccag cgcgttactg
     8041 cgtgccaccg cagcatcttc ggcggtgata tcttcagtcg cgagcagttg aggatggatg
     8101 gcgtccgggt acgtggagca caacgtttcc agcagcgtga gcgcctccga cagtcccctg
     8161 gcaccggcct gctggatacg gcagcgcagc aggacaacca gaatgcggat atctttgctg
     8221 cgcatcagca gacgatgggc gtcccgctca agctccgccc agttaatgtt ttccggcgta
     8281 ctgacaaaat cgccatattg cgcttcttca cgcggtgcgg cgcgggtgaa cagcagcagg
     8341 tattccggat cgtactccag atccgggccg caggggcatt ccgccgaaat tgggtcagac
     8401 aggaatattt ccatttaatt atcctcagtg cgccaggtac tgttctggtt cgaaaatcat
     8461 gcccgtcacc gggttgtcgt gcatggcttt acccgcccat gcagtccaac ccagttggta
     8521 cggaccaccg gtaacagccg gaggggcggc gtggggcttt agctgtagct caatttccca
     8581 gcagtattca taaccactaa aggcgcggac cagttctgtc aggacgggca ggttattgct
     8641 gccaggcaga aaacgtcggt aatcctccag cgtcagcggg ccgataacca gccggaactt
     8701 atactgcatg tccggcacgg cctggccgat gagtgcacca ttgccaataa cggaagaggg
     8761 ggcaggcatc cccagccggg tgatctcatc gtccgccacg gctatccagt gcaggacata
     8821 ctcctgtatc cggaaaggta caccgaaata gtgcgccagc gtggtggcca gaccgtccgg
     8881 attgcgggac tcacgcacca gatgggccga tgccgccaga cgagcgtgat ctggcaacgg
     8941 gctgtcagcg ctctcccgca aatcctgccc gctgaggctg gcgacataaa aggcaaagcg
     9001 gtcatgttgc ggtttatcca gaccgccccc agcggactgg gcgctgcgcc atgcctggta
     9061 aaactgcgtc agccagcggt gatgaaaaag atcagaaaaa cgggtcagcg tcggctttca
     9121 gtccggttac gcgccagttc ggtgtagtgc agcggcagtg gaccgttcgg cccccacagc
     9181 ccgaggctgt acagactgat gtgcaggcgc ccttcctgcc agcccacact ggcgatttct
     9241 cgtggcgcaa aggtcagcgc gggcgtttgt ccgaggcgaa atttttccat ccgtggttgc
     9301 caggtgttgc ccgccggaac ggtacacaac tgcacatcca cgcggcgcat caggttcaga
     9361 aaaccgtaac gccagggggg ggcctgcgcc tgagtgaaat cgttgctcat acgcttcccc
     9421 gctgtcccgc cctgacaggc caggtcataa ttttgccgcg ctgcatgctg tgcagcgtca
     9481 tctgcgagaa ggtattaatg gaaacatggc gggcgatata gtgttccagc accaggccga
     9541 acagatacgg actgatgccg gaaaaacctt cttcatccac cgtcagttca caactgacgc
     9601 cacgcccata gaccagtagt ccggaaccgg gcagacggcg ggtgaccggg ggcgttttac
     9661 aaccaatcag gctgcgcacc tggcgcgatt gcggactgtc atgagcagga ataaacaggt
     9721 tcagcagatc tcgcaacgcc tggccgccgg tacggtgatc cagatcggcc agcggcagat
     9781 aattaaaaga taactggcgg atcaaccgcc atgccatctc acgttcagcc agcgggggct
     9841 gcggcgagcg tggcgggcgg ataagcccta cgcccgccac cgggatggct gcatccacgg
     9901 ttagatcgtc ccggccattg cgggcaataa gacagggcag atcgcggtta gtgaccatgg
     9961 ccgtgatggt gatgtggcgc agattttccg ggtacggcgc ttcatactgg tcaaccagcg
    10021 acaggaacac ttccgagccc gtataggggg tacgggtgcc gtaacggcgg gcgttttctg
    10081 ataagcggcg gggctcccgg cgcagggaaa aataacgtcc gtgattgcct tcatcattat
    10141 tgcgggtgtg gtagagcggg cgaaaggcca tcttccgtgt ggtgtcggtt tccagccctt
    10201 ccacttcctg gaccgagaac acctcataat ccagcggatg ggttctgtcc accaccagat
    10261 gctgttccgt cgtgctgtgc gtgacatcga tccgggcggt gacgcgggga aacaggttga
    10321 taaccggggt acagaacagg ctgaactgtg ccgcatttgt ctggtggatc agccagtccg
    10381 gcggcaggcg gttcagcagg ataacaatct ccgccacgcc accgtcaatt ttctgtaatc
    10441 cggcagacag gcctgtcggg gtgaaaaaat agaagcgttc cggacaggca aaatattcat
    10501 gcagcaggtt atgaccgtga aacacgttcc acgccagcgg cagcagcccc tgatccggct
    10561 ccaggccttc gtacataacg ggctgttgca gattcacatc cagcgcgccg tcaaaatgcc
    10621 ccggaatgcc agccagggtc gcgatggcgc tggtatgtag aagttctaac aggtgtgagg
    10681 ccgtacgctc ctcgccgcag agataaaacg gcagcctgtc cagtccggca agctgactga
    10741 acgttagttc accaaaggtg cgcagggtaa tacgcagcgc cccggcgacg tgaatgttgg
    10801 ccggcaggta gcggtgcagg gcaggcatat ccggtggcgc ggcggtcagt cgcgcctctt
    10861 cgatggcgag cggccacagc gttacatcct ggctgctgcg aaactggcag gcagtgcttt
    10921 cgccttccgg aatggcggag aaaaaggctg tgtcgcgcgg gacggtaaat cctttcgcca
    10981 gatcgccttc ttccgtatcg ggatgcaact gcgccacgct cattgagggc gtgggtgtga
    11041 cgtaattggg gctgaccact tccagcaggc gctgggtgaa acgggggaat tcagcgtcaa
    11101 tttttagctg cgtgcgggcg gtcaggaagc tgaaggcctc gaccattcgc tctacatacg
    11161 gatcggcaat atccgttccc tgcatgccca gccgggcggc gactttggga tgacgggcgg
    11221 caaattcggc tcccgtttcc cgcagatagc tcagttcgcg gttgtagtat tccagtagcc
    11281 gtggatccat cggtctctct ctgaattaaa aaaacgtaat gcgactctgc tccatatcca
    11341 gcgcgctacg tacccggaat gcagtcggat acggctgcgt gaggatctcg ccgcgaattt
    11401 caaactgtaa tgtgttatgg ccactctggc ggcgctcatc ctgaagtggg atgatttgca
    11461 gagtggacgc tttcagccgg ggttcaaacc gggtgatggt tcgcaaaatg gccctgcgga
    11521 tatcgtccca cttgtgctca tacagaaagc tgcccgccag tggcggcagg ccgtagttaa
    11581 gtacggacgc tgccgcgtac ggataacgtg cagcatcaat atcatcttca tggctgacgg
    11641 tgttgagcag aaatgacaaa tcccggcgga tgatctcctt aatctgaacc ggactgacgc
    11701 tgataccgtg gtcgcgtttc tggtttgggg cgtcgtcaca caggcggtca aacagcgtgg
    11761 gcagcaggtg attgcctgga cgctggcggg aagcgctcac gcgtcgcttc cttcagcggt
    11821 gttaaaggta caggtggaca aatccagcag gctgatatcg ccgtgaccgg tgagccacac
    11881 cctctgaccc agcgcctgta ccagtgtctc tcccggaccg tcctgccaga ccgtttcccg
    11941 gcacagacgc agggagtcgg cagccttttc agagccgctg tagcggggaa acagctaggc
    12001 actgtgtgta tcgccattgt tcagggtgac gttgacgggt ttccagatca aatcgatcag
    12061 atgcgcaggc cgcggcgatt tcagcgaaca gatttgtgaa aacggcagcc agatatatgc
    12121 tccgcccgcg atcagttcga gtaccggacc ggtacgggag tcgctgtcgc taatccagtc
    12181 aaatgcgccg ccattccact gcccggaagt gtctgttatg gcctcaagcg cgcggctgcg
    12241 gtgagcatca gcttcctgcg cttcaccacg ggcgttacag gcgagggcgg tcagcagatc
    12301 ctccatccag gcgggcggcg ggagaataac accgggccgc tgttcgccct gaaagcaggc
    12361 gtggcggtat atctcacagc ggatcagctc gccaaatacc tgcgcttcgc gagtataatt
    12421 tgcgtccatc cgggcgcata gctggatctg ttgcagtgcc cgcgaccagt cgcctgccac
    12481 gcacaataac tgaaacaggc tgtggcggcg ggaggcctgc gctgggtgca tcctgacctg
    12541 ttgctctgcc atctgaatgc cctccaccac ggaataatcc cgcagcagcg ccgacaaggt
    12601 aatcggtagc tcgttattgt ttgtcatgat atatctctcc atgttgtgcc gggtttatcc
    12661 ggtaatggct gtcaatgcca atgatgcgat gctctttccg tgtcagatct ggcagtacgg
    12721 tgttatgcgc tgtttccggg gcgtattccg gggaaagtaa atgcaggata tcagggggag
    12781 tcttttcgac aggccagata gtttcatctt cgccagtggc atccagtaca ttgagaatag
    12841 cgtcaatgct taacccacca gtgaccatat cctgcaatgt ctcggcttcg gtgaccaggt
    12901 cacgcaatga atccagatcc tgcgaggagg gctcttcgtt aaaaggaaaa ggatcagcat
    12961 ggcgattaag atcttgttcg ggagataaca acgcctcgcg gtattcctga taaagtcggg
    13021 tcagggcatt tcctttttcc gcaccttctc cggtggagga ggctccatga acaggaatga
    13081 tatcgaatgg attttgtgat gtcagatgct gctgcctgaa ccaatccaga tccagatatt
    13141 cggttattgc ttccgacgtg atggcagtct gcgatatcgt ttttactgtg ctcaggttcg
    13201 gagatgatgg attgccatgt acaaggcact atgaagataa cccccattct atcaggtcgc
    13261 cttcattcag acgcatacgg tgatgaggtt ccacaatctg gtcgtttacc gtacagcaat
    13321 attcggtaga ctgattgata atccaccatg cttcttcgtg gcgggtcaga gtgagggcaa
    13381 tctttacatc atcattcacc ttaaaaacag gaatccaggg agaggtaacg gtgaagataa
    13441 tttgtgttcc ttcagtcgcg taaattgtgt tatcggcgat cccgtgcgcc tgtatttttt
    13501 ggagtcccca cgtaatattt ggtttcattg tg
//
LOCUS       DAOFMX010000101        13250 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.101, whole genome shotgun sequence.
ACCESSION   DAOFMX010000101 DAOFMX010000000
VERSION     DAOFMX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.101"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="QBA20_003490"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="QBA20_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDQ6293900.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="QBA20_003491"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="QBA20_003491"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDQ6293901.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="QBA20_003492"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="QBA20_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="QBA20_003493"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="QBA20_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDQ6293902.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="QBA20_003494"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="QBA20_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDQ6293903.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="QBA20_003495"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="QBA20_003495"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDQ6293904.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEVCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="QBA20_003496"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="QBA20_003496"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDQ6293905.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="QBA20_003497"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="QBA20_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="QBA20_003498"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="QBA20_003498"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDQ6293906.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9657..10103
                     /locus_tag="QBA20_003499"
     CDS             9657..10103
                     /locus_tag="QBA20_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDQ6293907.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10114..11565
                     /locus_tag="QBA20_003500"
     CDS             10114..11565
                     /locus_tag="QBA20_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDQ6293908.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDISKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11555..12625
                     /locus_tag="QBA20_003501"
     CDS             11555..12625
                     /locus_tag="QBA20_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDQ6293909.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12625..>13250
                     /locus_tag="QBA20_003502"
     CDS             12625..>13250
                     /locus_tag="QBA20_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDQ6293910.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTTRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3161 a         3457 c         3489 g         3143 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataagacca ttgtttttat tttgaaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa actacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg aggtgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccagctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagcg tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaattgatt aaaatatatt ttatggagtc cgattaatgg
     9661 cagctcttac tgcaagctgt attgacctga atattcaggg caatggcgct tattccgttc
     9721 tgaagcagtt ggcgacaata gcgttacaaa acggttttat caccgactca caccagttcc
     9781 tgcaaaccct gctgctgcgc gaaaaaatgc actctactgg atttggttcc ggtgtcgcag
     9841 tgccgcacgg taaaagcgcc tgcgttaaac aaccgttcgt attattcgcc cgcaaagcgc
     9901 aggctattga ctggaaagcc agcgatggcg aagacgtcaa ttgctggatc tgcctcggcg
     9961 tgccgcaaag cggcgaagag gatcaggtca aaatcatcgg cacactgtgt cgcaaaatta
    10021 ttcaccagga atttattcat caactgcaac agggcgatac cgaccaggtg ctggccttgt
    10081 taaatcaaac cctcagctca taaggaagtg gcgatggagt catccttacg tattgtcgcg
    10141 atcaccaact gccccgccgg gatcgctcac acctacatgg tggcggaagc cctggaacag
    10201 aaagcccgtt ctctcggcca taccataaaa gtggaaactc aagggtccag tggcgttgaa
    10261 aaccgcttat ccagcgaaga gattgccgct gccgattacg tcattcttgc taccgggcgt
    10321 ggcctgagcg gtgatgatcg cgcgcggttt gccgggaaga aagtttatga gattgccatc
    10381 tccctggcgt tgaaaaatat cgaccagatt ttcagcgaat taccgacaaa ctcgcagctt
    10441 tttgccgcag atagcggcgt gaagcttggc aaacaggaag tgcaaagcgg cagcgtgatg
    10501 agccacctga tggcgggcgt ctctgcggcg ctgccgtttg tcatcggcgg tggtatcctg
    10561 gtggcgcttg ccaacatgct ggtgcagttc ggtttgccgt ataccgatat ttcgaaaggc
    10621 gcgccgtcgt ttacctgggt agttgaatcc atcggttacc ttggcttcac ctttatgatc
    10681 cccatcatgg gcgcttatat tgcctcgtcg attgccgata aaccggcttt cgctccggcg
    10741 tttctggttt gctatctggc gaacgacaaa gcgctgctcg gcacccagtc gggtgcggga
    10801 ttcctcggcg cggtggtgct ggggctggcg attggctatt tcgtcttctg gttccgcaaa
    10861 gtgcgccttg gcaaagcatt acaaccgctg ctcggttcaa tgctgatccc gttcgttact
    10921 ctgctggttt tcggcgtgct cacttactac gttatcgggc cggtgatgtc cgacctcatg
    10981 ggcgggctgc tccacttcct gaataccatt cctccatcaa tgaagtttgc ggcagcgttt
    11041 ctggtcggtg cgatgctggc gttcgatatg ggcggcccca tcaataaaac cgcctggttc
    11101 ttctgcttct cactgctgga aaaacacatt tatgactggt acgccatcgt cggcgttgtc
    11161 gcgttgatgc cgcccgttgc ggcgggtctt gcgacgttca ttgcgcccaa actgtttacc
    11221 cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg gtgccacagt cgcaaccgaa
    11281 ccggctatac cttacgctct ggcagctccg ttgccgatga ttaccgccaa tacgctggcg
    11341 ggcggcatta ccggcgtgct ggtgatcgct ttcggaatca aacgtctggc acctggtttg
    11401 ggtatctttg acccgctgat cggcctgatg tcgccggtag gttcgtttta tctggtgctg
    11461 gcgattggcc tcgcgctgaa catttcattc attatcgtcc tgaaaggatt gtggttacgc
    11521 cgcaaagcga aagccgcgca acaggagctt gtccatgaac attgagttac tgcaacagtt
    11581 gtgcgaagcc agcgccgtta gcggcgatga acaggaagtt cgcgacatcc tgataaacac
    11641 gctggaacct tgcgttaatg aaatcacctt tgatggtctg ggcagctttg ttgcccgtaa
    11701 gggaaataaa ggtccaaaag ttgccgttgt cgggcatatg gatgaagtcg gctttatggt
    11761 cacccacatc gacgagagcg gttttctgcg tttcaccacc attggtggct ggtggaatca
    11821 gtcgatgctc aaccatcggg taaccatacg cacacacaag ggagtgaaaa tccctggtgt
    11881 gattggtttc gtcgcgcctc atgcgttaac ggaaaagcaa aggcaacagc cgctgtcatt
    11941 tgatgagatg ttcattgata ttggcgcaaa cagtcgcgat gaggtggaaa aacgcggtgt
    12001 ggcgatgggc gattttatta gcccggaagc caattttgcc tgctggggcg aagataaagt
    12061 ggtcggcaag gcgctggata accgcatcgg ctgcgcaatg atggctgaac tattgcagac
    12121 ggtgaataat cccgaaatta cgctgtatgg cgtcggcagc gtggaagaag aagttgggct
    12181 acgcggggca caaacctcgg cggaacacat taaaccggat gtagtgatcg tgctggatac
    12241 cgctgtcgca ggcgatgttc cgggcattga taacattaaa tacccgctga aactgggcca
    12301 ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc aaccagaaac tggtagcagc
    12361 gttaaaaaac tgtgccgcac ataacgattt accgctgcaa ttttccacca tgaaaaccgg
    12421 agcgacggat ggcgggcgct acaacgtgat gggcggcggg cgtccggttg ttgcgctgtg
    12481 cctgccaacc cgctatctgc acgctaacag cggcatgatt tcaaaagccg attacgatgc
    12541 tctgctcacg ttgatacggg gttttctgac gaccttaacc gcggagaaag tcaacgcgtt
    12601 tagccagttc cgtcaggtgg attaatgcta aacgaacgcc agttaaagat tgtcgatctg
    12661 ctggagcaac agccgcgcac gcctggtgag ctggcgcaac agactggcgt ttcaggcagg
    12721 accatcctgc gtgatattga ctatctcaac tttaccctta acggcaaagc ccgcattttc
    12781 gccagtggca gtgcgggcta tcagctggaa atcttcgagc gccgcagctt ttttcagttg
    12841 ctgcaaaagc acgataacga cgatcggctg ctggcgctgt tattactgaa tactttcact
    12901 acccgtgcgc aactcgcctc ggcgcttaat ttgccagaaa cgtgggtagc agagcgtctg
    12961 ccccagttaa aacagcgtta tgaacgcact tgttgcctgg ccagccgccc tggtttgggc
    13021 catttcattg atgagacaga agagaaactc gttatcttgc tggcgaactt gctgcgcaaa
    13081 gatccgtttt taattccgct ggcgggcata acacgagaca accttcagca tttatccacg
    13141 gcctgcgaca accaacaccg ctggccgctc atacagggtg attatctctc cagcctgatt
    13201 ctggcgattt acgccctgcg taatcaactg accgatgagt ggccgcaata
//
LOCUS       DAOFMX010000102        13029 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.102, whole genome shotgun sequence.
ACCESSION   DAOFMX010000102 DAOFMX010000000
VERSION     DAOFMX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.102"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(14..334)
                     /locus_tag="QBA20_003503"
                     /pseudo
     CDS             complement(14..334)
                     /locus_tag="QBA20_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(540..1259)
                     /locus_tag="QBA20_003504"
                     /pseudo
     CDS             complement(540..1259)
                     /locus_tag="QBA20_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1275..1607)
                     /gene="cusF"
                     /locus_tag="QBA20_003505"
     CDS             complement(1275..1607)
                     /gene="cusF"
                     /locus_tag="QBA20_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDQ6293911.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1631..3013)
                     /gene="cusC"
                     /locus_tag="QBA20_003506"
     CDS             complement(1631..3013)
                     /gene="cusC"
                     /locus_tag="QBA20_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDQ6293912.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRAWALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3170..3853
                     /gene="cusR"
                     /locus_tag="QBA20_003507"
     CDS             3170..3853
                     /gene="cusR"
                     /locus_tag="QBA20_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDQ6293913.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3843..5285
                     /gene="cusS"
                     /locus_tag="QBA20_003508"
     CDS             3843..5285
                     /gene="cusS"
                     /locus_tag="QBA20_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDQ6293914.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5435..7672
                     /gene="nrfB"
                     /locus_tag="QBA20_003509"
     CDS             5435..7672
                     /gene="nrfB"
                     /locus_tag="QBA20_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDQ6293915.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7659..10627
                     /gene="nfrA"
                     /locus_tag="QBA20_003510"
                     /pseudo
     CDS             7659..10627
                     /gene="nfrA"
                     /locus_tag="QBA20_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10628..11518
                     /locus_tag="QBA20_003511"
     CDS             10628..11518
                     /locus_tag="QBA20_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDQ6293916.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11701..12462
                     /gene="envY"
                     /locus_tag="QBA20_003512"
     CDS             11701..12462
                     /gene="envY"
                     /locus_tag="QBA20_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HDQ6293917.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12508..12584)
                     /locus_tag="QBA20_003513"
     tRNA            complement(12508..12584)
                     /locus_tag="QBA20_003513"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12548..12550),aa:Arg,seq:tct)
     gene            12827..>13029
                     /locus_tag="QBA20_003514"
     CDS             12827..>13029
                     /locus_tag="QBA20_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDQ6293918.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3185 a         3380 c         3312 g         3152 t
ORIGIN      
        1 ccggggcggt tcagttgtcg aaccagtagt cgagacactg ggcgaccgtc atggagttct
       61 ccaccttctc ttcaaaataa gtgcgtggat ccgttcctga gaaatagagc tttcgcaagt
      121 cagcagtgat ctgtctggca tccttcaaag acagggatgg gtatcgacca agcccaagtc
      181 gattaggctt gccatgccag cgatagcggt actggaactg gatgaccccc ttcggtgaaa
      241 ttcgtacgct gaggccatca gcatcagcca cttcttgtgg gccagaatat ggtttaccat
      301 aaatagtacg cagttttgtg tcgcttatag ccataaataa tatttggtac gctcagaaat
      361 aatggtttgg tacacatctg gtacacaata tcacatggac aacagcgcac agtaaacaac
      421 tatatttgac gttcgtagac atactcaggt gaaaaaaggg ttgtttttcg agttataaca
      481 gacagttaat taactacagt ggacaatgct agacaatgac agacacagat aagcaaccta
      541 ccttcctctt tcgcgaccac gttatctttg gcgatattca ttcccgcgcg cagatcaaac
      601 gcggtgatca cgccatcaat gggcgctttg agcgtaaagc gagtctggat tttttgcgtg
      661 gcgatcaggt ggcgaatatc cgcctccggc attcccgcca gtcgcagccg ctcaagaatg
      721 ccttcagtct gggtcgccgt accgccggtt tcgcgcaaca gtaaatactc actctgcgct
      781 tccacccagt cgggaatggt caggtcgaga agcggtgtgc ccttttgtac tttatcgccc
      841 acggtaagcg gatacacctt gtcgataaac ccggcagcgc gagcctgcac aatggcatac
      901 tgatactcgt tgtaactgac attcgccggg aaactctggg caaaagtcag cggcccgcgc
      961 gtgacggtag ccgttttcac ccccagattc tgcgtctgag tcgggtcaat gcgcacacca
     1021 gacgcagaac tctcttcatc ggcatatttc ggcaccagat ccatatccat aaacggcgat
     1081 ttacctggtt tatcgaaccg cgtattggga tacatcgggt cgtaccagaa taagatttta
     1141 cgttctgcgg tcggcatttt ttctgcgtgc ggttccgcct ggtcaaacca ggtaaaacct
     1201 gacgctgaaa taataccgcc cgcgatcatg ctgccgataa taagcgcgat ttttttcatc
     1261 tcattaaacc tgggttactg gctgacttta atatcctgta ataaagaaag gttgccctgc
     1321 tggacaaaat taaacgccac tttgtcgccg gttttaattt cactcatttt cgtctgcggg
     1381 gtgatggtaa agcgcatggt catctccggc cagttgacgg cagcaatcgg atcgtgatgg
     1441 atggtgattt ttttgctttc cagatcaaca ccttttacca caccggtggc actaatgacc
     1501 tgtggttgtg cttcgctcat ggtttcatga tgatgttcgt tagcctgggc attaaagcca
     1561 ataacggtaa acagactgaa cattgcgact tgcagtgctt ttttcattta ttctctcctg
     1621 gagttaaaaa ttattgctgc caaccgcccc ctaaggcggt atacaaataa atttcgttaa
     1681 cctgacgggc ataattcagg tcaagtaaag tttgtcgggt tgcaaataaa gaacgctcgg
     1741 catccagcac ttccagataa cttaccgcgc cgtgctgata taatgcccac gcccgttgca
     1801 gggtaatttg cagcgacgcc agataacgct gctgggcgct gatttgatcg tccaggcttt
     1861 gacgtaatgc cagcgcatct gccacctctt taaaggcgtt ctggattttc tgttcataat
     1921 tcaccaccga ctgctgctgg cgaatttcgg cgatatccag attggcctgg ttgcgtccgg
     1981 cattaaaaat ggggatctca attttaggaa taaaattcca catcccgctg ctagcgttaa
     2041 ataatgacga cagatcgctg ctggcggttg atattccgct ggtcaggctg atagacggga
     2101 aaaatgccgc acgcgcagca ccaatattag cattagccgc cattaacgcg tgttcagctt
     2161 ccataatatc cgggcgctgc aataagattt gcgacgacaa gcccgccggt aatttaacgc
     2221 tttgcaggct gccgctgttt actgtctgcg cttgcggtag cttgccgtag cttcccaata
     2281 acagttgcaa tgcattattc gcctgcgcca gttccccctg acgtttagcg atgtcactgc
     2341 gggtactttc tatcaccccg cgagcctgtt ccagcgccag aacattgctg ctaccggtca
     2401 acagttgttt ttcgacaaac gcatatgact gctgataatt acgcagcgtt tcttcggcta
     2461 tttgcaattg cgcatacgcc agctgctgat tgaaatagct ttgcgcgaca ttagaaacca
     2521 gcaaaatatg caccgcgcgc tgggcttcct cagtggctaa ataattttgg cgctcggctt
     2581 cgctcatgtt ctttaagcga ccgaaaaaat cgagatcaaa gctggcgtta aggccagtcg
     2641 agaactcccg cgtcgtggct gaatcgcctt taagattgcc gctccagctg ccgctgccct
     2701 cgccattgag ctgtgggtag cggtcggcat cggtcagacg atattgcgcc cgtgcctcct
     2761 gcactttcag cgtcgccatg cgcaaatccc ggttattcac cagcgcctcg ctaatcagcg
     2821 tcttcacctg attatcaaca aaaaaggtgc gccagcccac gttctggtag ttatccgctg
     2881 cgttaaccag gccgttctgg ctgagtgaga actgctgcgg tacgggcatt accggacgct
     2941 gataatccgg tgccagtgaa caaccagtta gcgcaagggc cacacaaaat ggcagaagtt
     3001 tacaaggaga cataggctca taatttctgg tgattttatg tcgccaactt tactcgccag
     3061 gctctgattt tccggtgaca ggaaaatgac aaaattgtca ttttgccaat aagcgattgc
     3121 catctgatcc cgctactcta gaattgcccg ggcaacatgc ggaggaaata tgaaactgtt
     3181 gattgtcgaa gatgaaaaga aaaccggaga atacttgacc aaagggttaa ccgaagccgg
     3241 ttttgtggtc gatttggccg acaacgggct gaatggctac catctggcga tgaccggtga
     3301 ttatgacctg ataatcctcg atattatgct gccagacgtg aacggctggg atatcgtgcg
     3361 catgttgcgc tccgccaata aagggatgcc gattctgttg cttaccgcgc ttggcaccat
     3421 tgaacatcgc gtcaaggggc tggagttggg ggcagatgac tacctggtga aaccgttcgc
     3481 ttttgctgaa ctgctggcgc gggtgcgcac tctcctgcgg cgcggggcgg cggtgattat
     3541 cgaaagtcag tttcaggttg ccgatctgat ggtcgatctc gtcagccgca aagtcaccct
     3601 cagcggcacg cgcatcactc tgaccagtaa agaatttact ctgctggagt tttttctccg
     3661 ccatcagggc gaagtgctgc cccgctcgct tatcgcctcg caggtatggg acatgaattt
     3721 cgacagcgac actaacgcca ttgatgtggc ggtgaagcgg ctacgcggca aaatcgacaa
     3781 cgactttgag ccgaagctga ttcaaaccgt gcgcggcgtg ggttacatgc ttgaggtgcc
     3841 ggatggtcag taagccattt cagcgcccgt tttcgctggc aacccgcctg acctttttta
     3901 tcagcctggc caccatcgcg gcgtttttcg cctttgcatg gatcataatc cactcagtaa
     3961 aagtgcattt tgccgagcag gacattaatg atttaaaaga gattagcgcc acccttgaac
     4021 gggtactaaa tcaccctgac gaaacgcaag cccgacgctt aatgacgctg gaagatatcg
     4081 tcagtggtta ttccaacgtg ttgatttccc tggcagatag ccacggcaaa acggtgtatc
     4141 actcccccgg tgcgcctgat atccgcgagt ttgcacgtga tgccataccc gataaagacg
     4201 cgcggggtgg cgaggtgtat ctcctttccg gcccgacgat tatgatgcca ggccacggtc
     4261 acgggcatat ggaacacagc aactggcgga tgattaactt gccggttggc ccgttggtgg
     4321 acggcaaacc gatttatacg ctctacatcg cgctgtcgat cgattttcat cttcattaca
     4381 taaatgattt gatgaataaa cttattatga ccgcatcggt aatcagcatc ctgatcgtct
     4441 ttatcgtgct gttggcggta cataaaggtc acgcgccgat ccgcagcgtc agccgtcaaa
     4501 tccagaatat cacctcgaaa gatctcgacg ttcgccttga cccgcagacc gtgccgattg
     4561 agctggaaca gctggtactg tcgttcaacc atatgatcga gcgtattgag gatgtcttta
     4621 ctcgccagtc caatttctca gcggatatcg cccacgaaat tcgcacacca attactaatc
     4681 tcattacgca aacggaaatc gccctcagcc agtcgcgcag ccagaaggag ctggaagatg
     4741 tgctctactc taatctcgaa gagctgacgc gaatggcgaa aatggtcagc gatatgctgt
     4801 ttctcgctca ggccgataac aaccagctaa tccccgaaaa gaaaatgctc aacctggcgg
     4861 atgaagtcgg caaagtgttc gattttttcg aggcgctagc ggaagatcgc ggcgtggagt
     4921 tgcggtttgt tggcgacaaa cgtcaggttg cgggcgatcc gttgatgctg cgtcgggcgc
     4981 taagcaacct gctttctaat gcccttcgtt atacgccaac cagagagaca attgtagtac
     5041 gctgtcagac ggtcgatcac caggtgcaag tttccgtcga aaaccccggt acgcccattg
     5101 cgcccgagca cttaccgcga ttgtttgacc gtttctatcg cgttgacccc tcccgccagc
     5161 gaaaaggtga aggtagcggt attgggctgg cgatagtgaa atcgattgtt gtcgcgcata
     5221 aaggcacggt tgcggtaacg tcagatgcgc gggggacaag gtttgttatc acattacccg
     5281 cttaattatt cagcaaacgg caacttttat aaccagtgta aaaataacgt gccgcaatag
     5341 tcatacatat taatggtaaa aagctgtcac aattcataaa aaaccttaat atacgccacc
     5401 ctaaacataa ccagcgttaa tgtaaggttt ttgtgtggac tggcttcttg atgtttttgc
     5461 tacctggctc tacggcttaa aagtaatcgc gataacgtta gcggtcatca tgttcatcag
     5521 cgggctggac gattttttta ttgatgtcgt ctactgggta cgccgcatta aacgcaagtt
     5581 gagtgtttat cgccgctacc cgcgaatgag ttaccgcgaa ctgtataaac cagatgaaaa
     5641 accgttagcg attatggttc cggcgtggaa tgaaacgggc gtcatcggca atatggccga
     5701 gctggcggcg accacgctcg actacgaaaa ctatcatatc tttgttggca cctaccccaa
     5761 cgaccccgat actcaacgtg atgttgacga agtgtgcgct cgctttccga acgtgcataa
     5821 ggtagtctgc gcgcgtcctg gtcccaccag caaagccgac tgcctgaaca acgtgctgga
     5881 cgccatcacc cagtttgaac gtagcgccaa tttcgctttt gctggtttta ttctgcatga
     5941 cgccgaagat gtgatttcac cgatggaatt gcgtctgttc aactatctgg tcgagcgtaa
     6001 agatctgatt cagatcccag tgtatccgtt tgaacgcgaa tggacgcact tcaccagcat
     6061 gacttacatt gatgagtttt cagaactgca tggcaaagat gttccggtgc gtgaagccct
     6121 cgccggacaa gtgcccagcg caggcgtcgg cacctgtttc agccgccgcg ccgtgaccgc
     6181 actgttagct gacggtaacg gtattgcttt cgacgtgcag agtcttactg aagattacga
     6241 cattggcttc cgcctgaaag aaaaaggtat gacggaaatt tttgtccgtt ttccggtggt
     6301 ggacgaagcc aaagaacgcg agcagcgtaa atttttacag cacgcacgga cgtcaaacat
     6361 gatctgcgtg cgcgaatatt tccccgatac cttttcgact gcggttcgac aaaaatctcg
     6421 ctggatcatc ggcattgttt tccagggctt taaaacccat aaatggacct ccagcctgac
     6481 gctgaactac tttctctggc gcgaccgcaa aggggcaatc agtaactttg tcagcttcct
     6541 cgcgatgctg gtgatgatcc agcttttgct gttgctggcg tatgaaagtt tgtggcccga
     6601 tgcctggcat ttcctttcta tttttagcgg cagcgcatgg ttaatgaccc tgctgtggct
     6661 aaactttggc ttgatggtta accgcatcgt gcagcgagtg attttcgtca ctggctacta
     6721 cggcctcacg cagggcctgc tttccgtcct gcgtcttttc tggggcaacc tgattaactt
     6781 tatggctaac tggcgcgtgc tgaaacaggt tcttcagcac ggcgatccgc gtcgcgtcgc
     6841 gtgggataaa acaacgcatg acttccccag cgtcactggc gatacccgct cgttgcgccc
     6901 gttaggtcaa attctgctgg aaaatcaggt catcactgaa gaacaactcg atacagcact
     6961 gcgtaatcgc gtcgaaggtc tacgcctggg cggttcaatg ctgatgcagg ggctgattag
     7021 cgccgagcag ctggcacagg cgctggcaga gcaaaacggc gtggcgtggg aatccatcga
     7081 tgcctggcag atcccgtcct cgctgattgc cgaaatgcca gcctccgtag cgctgcatta
     7141 tgcagtactg ccgctgcgtc tggaaaatga tgagttaatt gtcggcagtg aagatggtat
     7201 tgacccggtt tcgctggcgg ccctgacgcg taaagtcgga cgcaaagtgc gttacgtcat
     7261 tgttctgcga ggacaaattg tcacagggtt acgccactgg tatgcacgcc gacgcggtca
     7321 cgatccgcgg gcaatgttgt acaatgcggt tcagcatcag tggctcacgg aacagcagac
     7381 cggtgaaatc tggcggcaat atgtgccgca tcagttcctg ttcgccgaaa tactgaccac
     7441 gctcggtcat attaatcgtt cagcaattaa cgtgttgtta ttgcgccatg aacgcagttc
     7501 tctgccgctc ggcaagtttt tggtcaccga aggcgttatc agccaggaaa cgttggatcg
     7561 cgtcctgaca attcaacgcg aattacaagt ttcgatgcaa tcactattac tcaaagcagg
     7621 tttaaacaca gaacaggttg cgcaactgga gtccgaaaat gaaggagaat aaccttaatc
     7681 gcgtcatcgg atggtctggt ttactgctga cgtctttatt gagtaccagc gcactcgcag
     7741 acaatatcgg caccagcgca gaagagctgg ggctgagcga ttatcgccat tttgttattt
     7801 atccccgtct cgataaggcg ctgaaggcac agaaaaataa cgacgaagca accgccatcc
     7861 gcgaatttga atatatacac caacaggtgc cggataatat tccgctgact ttataccttg
     7921 cggaagccta tcgtcatttt ggtcatgatg accgggcgcg gctgttgctt gaggataaac
     7981 tgaaacgtca cccaggagat gcccgacttg agcgcagtct ggcggctatt ccagttgaag
     8041 tgaaaagcgt tacgactgtt gaagaactgc ttgcccagca aaaagcgggc gacgctgcgc
     8101 cgaccctgcg ttgtcgcagt gaagtcgggc agaatgccct gcggctggca caattacctg
     8161 tcgccagagc gcaactgaac gatgcgacgt ttgctgcatc gccggaagga aaaacgctgc
     8221 gaaccgatct gctgcaacgg gcaatctacc tgaaacaatg atcccaggca gatacgctat
     8281 acaatgaagc acgccagcag aacacattaa gcgcggcaga acgccgtcag tggtttgacg
     8341 tgcttcttgc cgggcagctg gacgatcgga tcctggcact gcaatcacag gggatcttca
     8401 ccgatcctca gtcatatatt acttacgcga ccgcgctggc ttatcgtggc gaaaaagcac
     8461 gcctccagca ttatctcatt gaaaataagc cgctgtttac cacgggcgca caagagaaaa
     8521 gttggctcta tctgttatct aaatacagcg ccaaccccgt tcaggcattg gcgaattata
     8581 cggtgcagtt tgccgataac cgccagtatg ttgttggcgc gacgctaccg gtgctgttaa
     8641 aagaaggtca gtacgacgca gcgcaaaaac tgctcgccac cctccccgcc aatgaaatgc
     8701 ttgaggagcg ttatgctgtc agcgtggcga cccgtaacaa ggctgaatct ctgcgtctgg
     8761 cacgattgct gtatcagcaa gaaccgacaa atcttccccg cctggatcaa ctgacctggc
     8821 aactgatgca gaacgagcag tcacgcgaag ctgccgattt attgctgcaa cgctatcctt
     8881 tccagggcga tgcgcgtgtc agccagactt taatggcgcg actggcgtct ctgctggaaa
     8941 gtcatcctta cctagcaacg ccggcgaagg tggcgatttt atcgaaaccc ttaccgctgg
     9001 cggagcaacg tcagtggcaa agtcagttgc cgggtattgc agataattgc ccggcaatag
     9061 ttcgcttgct gggcgatatg tcgccttcct acgatgctgc cgcctggaac cgtctggcaa
     9121 agtgttatcg ggacacgcta cccggtgtgg cgttgtatgc atggcttcag gccgaacaac
     9181 gacaaccgag cgcctggcaa catcgtgcgg tagcctatca ggcgtatcag gttgaggact
     9241 acgccaccgc actggcggcc tggcagaaaa tcagtcttca cgacatgagc aatgatgatc
     9301 tgctcgccgc cgccaacacc gctcaggcag cgggaaatgg cgcggctcgc gatcgctggc
     9361 ttcaacaggc agaacaacgt gagctgggaa acaatgctca atactggtgg ctgcatgcgc
     9421 gacgttatat cccagctcag ccagaactcg cactgaacga tctcacgcgc tcaatcaata
     9481 ttgccccttc tgccaactct tacgttgcgc gggcgacaat ttatcgccaa cgtcataatg
     9541 ttccggccgc ggtgagtgat ttgcgcgccg cgctggaact ggaaccaaat aatagcaaca
     9601 cccaggcagc gcttggttac gccttgtggg atagcggtga tatcgcacag tcgcgggaaa
     9661 tgctcgaaca ggcgcataaa gggcttccgg acgatccggc actgatccga caactggcct
     9721 acgtgaacca gcgtctggat gacatgcctg cgacgcagca ctacgcccgg ctcgtgattg
     9781 atgacattga taatcaggca taaccccact gaccccagag caaaatcagc aacgcttcaa
     9841 tttccgccgt ttgcatgagg aggtcggtcg ccgctggacg ttcagtttcg attcttccat
     9901 cggcttgcgt tccggcgcaa tgagtaccgc taacaataat gtcggcggtg cagcgcctgg
     9961 gaaaagctat cgtagctacg gacaactgga agccgagtac cgcatcggac gcaatatgct
    10021 gctggaaggc gacctgctct cagtttatag ccgcgtcttt gccgataccg gagaaaacgg
    10081 ggtgatgatg ccggtgaaaa atccgatgtc cggcaccggt ctgcgctgga agccgctgcg
    10141 cgatcagatc tttttcctcg ccgtcgaaca gcagttgccg ctgaacgggc aaaatggcgc
    10201 atccgatacc atgctgcgcg ccagcgcctc attctttaat ggcggcaaat acagcgacga
    10261 atggcacccg aacggttcag gctggtttgc ccaaaacctg tacctcgatg cggcgcaata
    10321 tatccgccag gatattcagg cgtggacggc agattatcgc gtcagctggc atcagaaggt
    10381 agctaacgga cagactattg agccttacgc tcacgttcag gacaacggct atcgtgataa
    10441 aggcactcag ggcgcgcagc ttggcggtgt cggggtccgc tggaatattt ggaccggcga
    10501 gacgcactac gacgcctggc cgcacaaagt cagtctcggc gtcgagtatc aacatacctt
    10561 taaggcgatt aatcaacgta acggagagcg caacaacgcg tttctcacca ttggagtgca
    10621 ctggtaaatg cgtaagttca ttttcgtatt gctgacactg cttttggtca gccctttttc
    10681 ctttgcgatg aaaggtatta tctggcaacc acaaaaccga gatagtcagg ttaccgatac
    10741 ccagtggcag gggctgatga gtcagttacg ttttcaaggc ttcgataccc tggttttgca
    10801 atggacccgt tacggcgatg catttaccca gccagaacag cgcacgttat tgtttaagcg
    10861 tgccgcagct gcgcaacaga ctggcctgaa gctgattgtc gggctgaacg ccgatccgga
    10921 attttttatg caccagaaac agtcgtctgc agcgctggaa agctatctta atcgcctgct
    10981 ggctgccgat ctccagcaag ccagattatg gagcgccgcg cctggcataa cgccggatgg
    11041 ctggtacatc agcgcggaaa ttgacgacct gaactggcgc agcgaagccg cccgtcaacc
    11101 tttgctaaca tggttaaaca acgcgcagcg gctgattagc gatgtttcag caaaaccggt
    11161 ttatatcagt agttttttcg ccggaaacat gtcgcccgat ggctatcgcc aactgctgga
    11221 acaagttaaa gcaaccggag ttaatgtctg ggtacaggat ggcagcggcg tggataaact
    11281 taccgctgaa cagcgtgaac gttatctaca ggccagcgcc gattgccaaa gctccgcccc
    11341 agcaagcggc attgtttatg aactgtttgt cgccggcaaa ggcaaaacct ttacagcgaa
    11401 accgaaaccg gacgcagaaa ttgcctcgct gttagcgaaa cgttcctctt gcggtaaaga
    11461 catgctctat ttctctctgc gctatttgcc cgtcgcgcac ggcattctcg agtattaaat
    11521 cccctccagg taagtcgggt acgacctggc ttacctcttt cgctctcgtc agaattccat
    11581 caagattaat tgctaaaaag catgctaaag aaagaagatc gttatcggcg aacgggccga
    11641 tcactcatgc aggaagcata gatcctttga gcggagtttc tgatatctga ggagtgcgaa
    11701 atgcaattga gcagcagtga accttgcgtg gtgatcctga ccgaaaaaga ggtagaggta
    11761 agcgtcaata accatgctac gtttaccctt ccgaaaaact acctggccgc cttcgcgtgc
    11821 aacaataacg tcattgaact ctcaacgtta aatcacgtat taatcaccca catcaaccgt
    11881 aacatcatca acgattatct gttgttttta aataagaaat taacctgtgt aaagccctgg
    11941 tcgcggctgg caaccccggt tatcgcttgt catagccgta caccggaagt gttccggcta
    12001 gccaccaacc acagcaagca gcaatccagc aaaccctgcg aagcggagtt gacgcgcgca
    12061 ttacttttta ccgtattgtc taactttctt gagcaatcgc ggtttattgc cctactgatg
    12121 tatatcttac gcagcagcgt ccgcgacagc gtctgccgca ttattcaaag cgatattcag
    12181 cattactgga atctgcgaat tgtcgccagt tcgctatgtt taagccccag cctgctcaaa
    12241 aagaaattaa aaaacgaaaa taccagctat agccagattg tcacagagtg tcgtatgcgt
    12301 tacgccgtac agatgttatt gatggataac aaaaatatca ctcaggtggc gcaattatgt
    12361 ggctatagca gcacgtcgta ctttatctct gtttttaagg cgttttacag cctgacaccg
    12421 ttgaattatc tcgccaaaca gcgacaaaaa gtgatgtggt gaagggcaaa gcggaaacgg
    12481 ataagacggg cataaatgaa gaagaaatgg cgcgccctgc aggattcgaa cctgcggccc
    12541 acgacttaga aggtcgttgc tctatccaac tgagctaagg gcgcgttgat accgcaatgc
    12601 ggtgtaatcg cgtgaattat acggtcaacc cttgctgagt caatggcttt tgatctggtt
    12661 gctgaacaag tgaacgaccg cgtctgattt tctgatttat ttcgctatag cggcaaacaa
    12721 acgcacaccg ctgcgcgtct gaatcaagaa aacccgtatt ttcatgtatc aaagtacaat
    12781 ttaccgccct aacggaaaat tgtccgctcc tatgagactg gtaactatga aaccaacgtc
    12841 ggtgatcatt atggatactc atcctatcat cagaatgtct attgaagttc tgttgcaaaa
    12901 aaacagtgaa ttgcagattg tcctgaaaac ggatgattac cgcataacca tcgattatct
    12961 ccgaacccgt cctgttgatt taatcattat ggatatagac ttgcccggaa cagacggttt
    13021 taccttcct
//
LOCUS       DAOFMX010000103        12960 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.103, whole genome shotgun sequence.
ACCESSION   DAOFMX010000103 DAOFMX010000000
VERSION     DAOFMX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.103"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(184..1635)
                     /gene="uxaB"
                     /locus_tag="QBA20_003515"
     CDS             complement(184..1635)
                     /gene="uxaB"
                     /locus_tag="QBA20_003515"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDQ6293919.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1862..3280)
                     /locus_tag="QBA20_003516"
     CDS             complement(1862..3280)
                     /locus_tag="QBA20_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDQ6293920.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3434..3778)
                     /locus_tag="QBA20_003517"
     CDS             complement(3434..3778)
                     /locus_tag="QBA20_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDQ6293921.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3778..4704)
                     /gene="glsB"
                     /locus_tag="QBA20_003518"
     CDS             complement(3778..4704)
                     /gene="glsB"
                     /locus_tag="QBA20_003518"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDQ6293922.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4768..5703)
                     /locus_tag="QBA20_003519"
     CDS             complement(4768..5703)
                     /locus_tag="QBA20_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDQ6293923.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5783..6898
                     /gene="yneK"
                     /locus_tag="QBA20_003520"
     CDS             5783..6898
                     /gene="yneK"
                     /locus_tag="QBA20_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDQ6293924.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRPLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            7048..8238
                     /gene="ydeA"
                     /locus_tag="QBA20_003521"
     CDS             7048..8238
                     /gene="ydeA"
                     /locus_tag="QBA20_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDQ6293925.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8263..8928)
                     /gene="marC"
                     /locus_tag="QBA20_003522"
     CDS             complement(8263..8928)
                     /gene="marC"
                     /locus_tag="QBA20_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDQ6293926.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9140..9574
                     /gene="marR"
                     /locus_tag="QBA20_003523"
     CDS             9140..9574
                     /gene="marR"
                     /locus_tag="QBA20_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDQ6293927.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9594..9977
                     /gene="marA"
                     /locus_tag="QBA20_003524"
     CDS             9594..9977
                     /gene="marA"
                     /locus_tag="QBA20_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDQ6293928.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            10009..10227
                     /gene="marB"
                     /locus_tag="QBA20_003525"
     CDS             10009..10227
                     /gene="marB"
                     /locus_tag="QBA20_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDQ6293929.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10258..11157)
                     /gene="eamA"
                     /locus_tag="QBA20_003526"
     CDS             complement(10258..11157)
                     /gene="eamA"
                     /locus_tag="QBA20_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDQ6293930.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11421..12539
                     /gene="ydeE"
                     /locus_tag="QBA20_003527"
     CDS             11421..12539
                     /gene="ydeE"
                     /locus_tag="QBA20_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDQ6293931.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12666..12761
                     /gene="mgtS"
                     /locus_tag="QBA20_003528"
     CDS             12666..12761
                     /gene="mgtS"
                     /locus_tag="QBA20_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDQ6293932.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3411 a         3310 c         2964 g         3275 t
ORIGIN      
        1 ctcacgtggc ataaggcgtg gacagcggta tcgtctggat tttcaatttc ctatatgtca
       61 agcttacaac attaacaggc gctgcatgtg acacctgtta atgataaagg gatataggat
      121 gttgtaacta acatagtgaa ataagaaacc cggtcgaaac cgggttcaga agtaacggtg
      181 ttattagcac aacggacgta cagcttcgcg catccctttt tcaagaatcg catccaggtc
      241 attagcaacc tgctcgacca gaccaggcac ttgcgtcagg tcctgctccc agtggtcttt
      301 ttctgccaac acaatcgcta ccagttcctg ggtaccgata acacggtcac gatgctggct
      361 ccagagttgc tgataacgtt ccagccagtg tgcatcgtcc tgtaccggat atgtttctcc
      421 gttacgctca ccacgataga acgcaatcaa tgccgctaat gcgaaagtaa ggcgcgccgg
      481 aagtgtgcca tttgccttct gccctgccag cagctgcggc aggatgcggg tgcggaactt
      541 ggtcataccg ttgagcgcga tagacagcag ctgatgctta atgtacgggt tacggaaacg
      601 cccggtgact gcactggcga aagattccag ttcatcacga ggcaaatcca gtaccggaat
      661 aatttcttcg tagatggctt tttcaacgaa tgcgcaaatt tcagcatcgt tcatcgcttc
      721 acctacggta tccagccctg cctggaacgc caccggcacc agcgcggtgt gcgcaccgtt
      781 gaggattgcc actttgcgtt ctttatacgg tttaatatcg tcaacaatca gcacgttgag
      841 cggatatttg tccaggcgca gctcagtcgc taaggatttc ggcccctgaa tcacaaacag
      901 gtaaaagtgt tcagcggtgt cgaggaaacc atcgtgataa cccagttctt cttccagttt
      961 agccacttca tcgcgcggat aaccggtaac gatacggtcc accagcgtag aacagaagct
     1021 gttagcctga tccagccatt gaataaatgc ttctggcagt gcccactctt gcgcatagcg
     1081 cagcaccagt tcacgcaacg cgtcgccatt gtagtcaatc aactcacacg gaatgatgat
     1141 ccaaccttta tccagcgcac cgttgaaatg gctaaagcgt tcgaacagca gacgggtcag
     1201 ttttgccgga tagcttaccg ctggcgcgtc atcgaattta tcgcccgcat ggtagctgat
     1261 acccgcttcg gtggtgttag agaaaacaaa gcgcatttcc gggttgtgcg ccagtttcag
     1321 gaattcatcg tattcactgt agacgctgat ttcacgatta accgagcgaa tcagacgcgc
     1381 gtcgctgacc gcttccccct tctcattcag gccacggata atggtggtgt acagaccatc
     1441 ctgcgtgctc agtgacggcg ggaatgaagt ttcaatcgga cgaacaacga ccacgccaga
     1501 attcagatcg gtgtgctcat tcaggagatc gatttgccag tcaacaaagg cgcgcaggaa
     1561 gttaccttca ccaaactgaa tgatacgttc tggatactgt gcaccgggaa aatcgcgacg
     1621 atttagtgtt ttcacaatgg gttcccttct gattagtcat acaacctgtt tgaattggtg
     1681 cgacaggtta gcaaacttta atacgccgaa cccctgtttt gatcaactcc tgatgattaa
     1741 tgagcagttt tatgagaaaa gtgtggcgcg gatcatggtt taatcgagga aatacgcctt
     1801 ttcctggatc ataaagtggt agaacacatt gcattcaaat cgcgcgaaat gaataaagat
     1861 gtcagacaac ttcctcaccg taacgcatag tgctggtacg gttgcgccca tctttcttcg
     1921 agcgatacag acacgtatcg gcttcgacca tcaatttatt aaaatcatcg gtcagcgttc
     1981 ggtacgatgc gcgaccacta ccgacgccaa tacttaccgt gagataaagc gttttttgtt
     2041 gccaggtgaa tggttgcagt tcaacgcctt tacggatttt ttccgccatt agcagaccat
     2101 cgacaggatt caccgacggc actgcaacag caaattcttc gccgcccatg cgcgccacca
     2161 gccccttatc accgacaatc ttctgaatat gccgggcaaa cacgcttaac actttatcgc
     2221 cacattcatg tccatagtta tcgttaatgc ttttgaaata gtcgatatca agcagcatga
     2281 ccgtcagatg ttgtgtctgt ttcagcgatg gacttttcag cgcctcataa agaccggacc
     2341 gtgagtaaac ctgagtcaga aaatcaaagt cggctcgcag cgcaacttgc ttcattagca
     2401 aattgatcgc tgccacgcta aaagaaacca taattgggca tatcgccatc gtggcaatac
     2461 cgagacgtgc ggagaacatt tccggaatgg agaacggcga accgaccgag atatcaatca
     2521 ccgaatttgc caccagtacg atttccaccg caccggtgac aaaggtcaac agacatgtta
     2581 cctgcggcgt atagcgcact gcacaccaga ttaatgcagg cagcgggaac gccagactcc
     2641 ccgctccgcc aatgactacc gaggcaataa ccgacacaat aagcgcgatg gcgggcatca
     2701 tctgttctgc tttaaagcgc ggcagcactc caggaatagc caacgtcagc atacaaggca
     2761 cgatcaacac gcccgttgag aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat
     2821 ccagactatc aatactgacc gaaccaatcg ccccgacaat agcgcataat aatgcggcaa
     2881 tcagacagta attaaatagc cgtaaagcac ttactggctc atacttattt ttccccagac
     2941 gcttatcccg cacgaccagt aaggcgacgg taacaataaa catcatattg gagaaattga
     3001 taaccagtga aaccagtccc cattcggtgg ttatggcatc ataaaccagc atcgccacat
     3061 aactgatcgc atagtaatgc agtcgattaa gccagacata gcgggcaaac acccccgcca
     3121 ttacgccatt cagcggccag aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa
     3181 taaaatagaa cagcgtggtt agcgcaaaaa tagcgatgct attacgtagc agatgacctt
     3241 cctggaacaa acggaaagtt gaaatggggt gtacatgcat taattatctg tccatacaat
     3301 tgcgggtggc aattatctca acgtgataag taaattctca taaaggatag catgttcgct
     3361 aacagcggcg gcaaataaag gtaccctggg aatgctattt tatcgttttc cgcaattctc
     3421 atggctgatt catctataca accagccagt gatagattac cgccacgata taacgttgtt
     3481 gttcttcgct taacgaaaca ccctgcggaa tgttatgcca cttagccaga caaccgcgac
     3541 agcaggtcgc ggtggcgtgc tgagcgataa acaccggatg cccgcgcatg ggggtttgct
     3601 taccgtcatt agccggtaat gccggagcaa gacgtttagc gacaaaatcc gccgcatgtt
     3661 gttcgatgac tggcgcgcct ttctccaggc aatactgacg ctctttcatg cccagacgaa
     3721 agcgagagcg aaattttgaa cgggataaac gcgcgaagag tggatcgaga gactgcatta
     3781 ataaaccgaa cgccctaact gtttcgtcaa ttgttcaagg acggcgatac ccgcaagcga
     3841 gttacctgca tcatccagtt ccggactcca gactgcgatg gccatttcat gcggaacaat
     3901 cgccacaata ccgccaccaa cgccagattt cgccggtagt cccacccgcc aggcaaactc
     3961 ccccgcgttc tggtacatac cactggtcgc catcagcgcg ttaatttgcc gcgcctgcat
     4021 tggcgtcacc acaggttcat caatatgaat agctttcccc tgattagcca gaaagacaaa
     4081 cgtccgggcc agctctacac agctcatttt cagagcgcag taatgaaagt agttttgcag
     4141 aacggttgtc acgtcatgat ggaaattgcc aaacgacttc atcagccagg cgatagccgc
     4201 atttcgcgcg gaatgttcaa attcggaacg cgctaccacc gtatcgtatg aaatatcaga
     4261 cacaccgctt aagccgcgca cgacttccag catacgttgc cgtggtgcgc ttagtcgccc
     4321 ttgcaacata tcgcagacca ccagcgcacc ggtattaatg aacggattac gcggaatacc
     4381 ctgttccatt tccagttgca ctaaagaatt gaacggtgat ccagacggat ctttgccgac
     4441 gcgttgccag atttcctctt cggagtaatg acgcatggcg acaacgagac tcagcacttt
     4501 ggaaatagac tgaatggaaa aacgttcttg cgcgtctccg gcctgaaaaa gctgtccgtc
     4561 aacggtacag atagcaatcc ccaatcggga accgtctact gtagccagcg ccggaatata
     4621 atccgcgact ttaccctgac caatgagcgg ccgcacttgc cgcaagatgt tttctaaaat
     4681 tgcattatcc atggcgactg ccactttcta ctcctggacc gcaggtctga aaagacctgc
     4741 gagtatatca gagctgaata tgtcgcgtca gatccggtct ttccacactg tctggatatt
     4801 acagaattcg tgtaagccga aatgggaaag ctcacgacca aagccactct ttttcacgcc
     4861 accaaaggcc actcgcgcgt cgctggcaca ataaccattg ataaacaccc caccgcattc
     4921 cagacgtgcc gccatctgtc tggcctgtgt ttcgtcggtg gtaaaaatgg tcgctgaaag
     4981 gccgaactca ctatcattag ccagttccag cgcatgttct gcatctttcg caatggtgat
     5041 tgcagcaacg gggccaaaca tttcttcccg aaacgcggtc atttctgggg taacattcgc
     5101 cagaaccgtt ggcggatagt agttacctgc tccagccatc ttttccccgc ccagtaacaa
     5161 acgcgcaccc tgcgccaggg ttttctccac ctgatgatgc agctcatcac gtaaatcaaa
     5221 acgagccatt ggtccgagag cgttctcttc atcacgggga tcgcccattt tcaaggctgc
     5281 cgcagctgac acaaaacgtt cggtaaatgc cgaagcaatt ccctcttcga taataaagcg
     5341 ttttgccgct gcacaaacct gtccggtatt ctgataacgt ccggctaccg ccgctttaac
     5401 cgccagttcc agatcggcat cgttaagcac aataaatgga tccgaaccgc ccagttccag
     5461 tacgcatttt ttcagtgccg ctccagcacg aacacttccg gtcaccgtga cagcagcaat
     5521 gcgcgagtct ttaatcatct gactgacacc gtcgttgtcg gcattcagcc agccatatac
     5581 gccttgcggg attcccgcat ctttaaacac ttgggcaatg agctgagcac agcccatcac
     5641 attcggcgca tgtttaagta agtagccgtt acctgcaaga atgatgggaa cagcgccacg
     5701 catcacctgc ctttgatagt tcttgcagct caaatgccct atatagtgat gttccgatga
     5761 cttatgacta tatggggcaa atatggttac gccagtaagc atcagcaatt acatatctct
     5821 tcctgatgat tttcccgctc gtaatattgc acctcaagta aaagaagttt taaaggattt
     5881 tattgatgca ctcagtacca taatatgtga tgaagagtgg cgcacgtctt taaacaccaa
     5941 ctcagccaca aaaaagatat ttaacaacct tgataactta tcatatattc agaggacttc
     6001 ttttcggggt aacgacacac tatacaatga aaaggttcag tttaaactca cttatcctgt
     6061 caagaatgga agacacaaag aaaatattga atttcaggta gtaataaatt taagccctat
     6121 ttatttagat aattttcgcc atgatggaga aattaatatt ttttgcgctc ccaacccaaa
     6181 gcctgtcact atggggcgcg tatttcagac cggcgtcgag cgtgtgctct ttctgtttat
     6241 gaatgatttt attgaacaat ttccaatgat caaccttggc gcccccatca aaagagcgca
     6301 tacaccacat attgaacccc tgcctccgga tcaccatacc gctgcagatt acttacgcca
     6361 gtttgatttg cttgtcctga attttatctc tcgcggtaat tttgtcatac tcccccgatt
     6421 atggaataac tctgaggttc acagatggtt cgtcaataaa gatcctaact tgatcaccgc
     6481 cattctcgac ataacggaca gcgaattaaa agaggattta ttgcaaagcc tgatggattc
     6541 attaggttct aacaaacatg tacaacccga agtctgcatc tgcttcttat cccttttagc
     6601 agaacaagaa tctcctcatt ttcaggactt gtttttattt ttcgccaata tgttattgca
     6661 ctatcaccaa tttatgaatc ccaatgaaag tgatctgaat gacgtgttga tgccagcatc
     6721 attaagtgat gataaaatta tcaaacatat ggcgcgcagg cccctcaaac tgtttgtaaa
     6781 aaatgaaacg ccgccaaaag ttactcacga agacctggtg aaaaacaggc ctcgctcccc
     6841 tgtcagacca cctatacccg caaccgccaa aacgccagac ctccctgaac gtcattaaac
     6901 cgtgatgtta ccgactctct gactcggaac agaatcagcg tcagagaaac ggaaaacgcg
     6961 atccagatca caaatgcatt gtattcacat cattaaccgt tttaagatca tttcatcact
     7021 ttttcgcaac tcacccgata atctgttatg acaacaaaca ctgtttcccg caaagtggcg
     7081 tggctacggg tcgttacgct ggcagtcgcc gccttcatct tcaacaccac cgaatttgtc
     7141 cctgttggcc tgctctctga cattgcgcaa agttttcaca tgcaaaccgc tcaggtcggc
     7201 atcatgttga ccatttacgc atgggtagta gcgctaatgt cattgccttt tatgttaatg
     7261 accagtcagg ttgaacggcg caaattactg atctgcctgt ttgtggtgtt tattgccagc
     7321 cacgtactgt cgtttttatc gtggagcttt accgttctgg tgatcagtcg cattggtgtg
     7381 gcttttgcac atgcgatttt ctggtcgatt acggcgtctc tggcgatccg tatggctccg
     7441 gccgggaagc gggcacaggc cttgagttta attgccaccg gtacagcact ggcgatggtc
     7501 ttaggtttac cgctcgggcg cattgtgggg cagtatttcg gctggcgaat gaccttcttc
     7561 gcgattggta ttggggcgct tgttaccctt ttgtgcctga ttaagttact tcccttactg
     7621 cctagtgagc attccggttc attgaaaagc ctcccgctat tgttccgccg cccggcattg
     7681 atgagcattt atttgttaac tgtggtggtt gtcaccgccc attacacggc atacagctat
     7741 atcgagcctt ttgtgcaaaa cattgcggga ttcagcgcca actttgccac ggcattactg
     7801 ttattactcg gtggtgcggg cattattggc agcgtgattt tcggtaaact gggtaatcaa
     7861 tatgcgtctg cgttggtaag tacggcgatt gcgctgttgc tggtgtgtct ggcactgctg
     7921 ctacctgcgg cgaacagtga aatacacctc ggggtgctga gtattttctg ggggatcgcg
     7981 atgatgatca tcgggcttgg tatgcaggtt aaagtgctgg cgctggcacc agatgctacc
     8041 gacgtcgcga tggcgctatt ctccggcata tttaatattg gaatcggggc gggtgcgttg
     8101 gtaggtaatc aggtgagttt gcactggtca atgtcgatga ttggttatgt gggcgcggtg
     8161 cctgcttttg ctgcgttaat atggtcaatc attatatttc gccgctggcc agtgacactc
     8221 gaagaacaga cgcaatagtt gaaaggccca ttcgggcctt ttttaatggt acgttttaat
     8281 gatttccagg atgccgttaa taataaactg cacacccata cataccagca ggaaccccat
     8341 caggcgggag atcgcttcaa tgccaccctt gcccaccagc cgcataattg cgccggagct
     8401 gcgtaggctt ccccacaaaa taaccgccac caggaaaaag atcagcggcg gcgcaaccat
     8461 cagtacccaa tcagcgaagg ttgaactctg acgcactgtg gacgccgagc taataatcat
     8521 cgctatggtt cccggaccgg cggtacttgg cattgccagt ggtacaaagg caatattggc
     8581 actgggttca tcttccagct cttccgactt gcttttcgcc tccggtgaat caatcgcttt
     8641 ctgttgcgga aaaagcatcc gaaaaccgat aaacgcgacg attaagccgc ctgcaattcg
     8701 cagaccggga atcgaaatgc caaatgtatc catcaccagt tgcccagcgt aatacgccac
     8761 catcatgatg gcaaatacgt acaccgaggc catcaacgac tgacgattac gttcggcact
     8821 gttcatgttg cctgccaggc caagaaatag cgcgacagtt gttaatgggt tagctaacgg
     8881 cagcaacacc accagcccca ggccaattgc tttaaacaaa tctaacattg gtggttgtta
     8941 tcctgtgtat ctgggttatc agcgaaaagt ataaggggta aacaaggata aagtgtcact
     9001 ctttagctaa ccttgcatcg cattgaccaa aacttgaacc gatttagcaa aacgtggcat
     9061 cggtcaattc attcatttga cttatacttg cctgggcaat attaccccct acaactaatt
     9121 acttgccagg gcaactaatg tgaaaagtac cagcgatctg ttcaatgaaa ttattccatt
     9181 gggtcgctta atccatatgg ttaatcagaa gaaagatcgt ctgcttaacg agtatctgtc
     9241 tccgctggat attaccgcgg cacagtttaa ggtgctctgc tctatccgct gcgcggcgtg
     9301 tattactccg gttgaactga aaaaagtgtt gtcggtcgac ctgggagcac tgacccgtat
     9361 gctggatcgc ctggtctgta aaggctgggt tgaaaggttg ccgaacccga atgataagcg
     9421 cggcgtactg gtaaaactta ccaccagcgg cgcggaaata tgcgaacaat gccatcaatt
     9481 agttggccag gacctgcatc aagaattaac aaaaaacctg acggcggacg aagttgcaac
     9541 acttgagcat ttgcttaaga aagtcctgcc gtaaacaaaa aagaggtatg acaatgtcca
     9601 gacgcaatac tgacgctatt accattcata gcattttgga ctggatcgag gacaacctgg
     9661 aatcgccact gtcactggag aaagtgtcag agcgttcggg ttactccaaa tggcacctgc
     9721 aacggatgtt taaaaaagaa accggtcatt cattaggcca atacatccgc agccgtaaga
     9781 tgacggaaat cgcgcaaaag ctgaaggaaa gtaacgagcc gatactctat ctggcagaac
     9841 gatatggctt cgagtcgcaa caaactctga cccgaacctt caaaaattac tttgatgttc
     9901 cgccgcataa ataccggatg accaatatgc agggcgaatc gcgcttttta catccattaa
     9961 atcattacaa cagctagttg ataacgtgac aacgtcactg aggcaatcat gaaaccactt
    10021 tcatccgcaa tagcagctgc gcttattctc ttttccgcgc agggcgttgc ggaacaaacc
    10081 acgcagccag ttgttacttc ttgtgccaat gtcgtggttg ttcccccatc gcaggaacaa
    10141 ccaccgtttg atttaaatca catgggtact ggcagtgata agtcggatgc gctcggcgtg
    10201 ccctattata atcaacacgc tatgtagttt gttctggccc cgacatctcg gggcttatta
    10261 acttcccacc tttaccgctt tacgccaccg caagccaaat acattgatat acagcccggt
    10321 cataatgagc accgcaccta aaaattgcag acccgttaag cgttcatcca acaatagtgc
    10381 cgcacttgcc agtccgacta cgggcaccag taacgataac ggtgcaaccc gccaggtttc
    10441 atagcgtccc agtaacgtcc cccagatccc ataaccaaca attgtcgcca caaacgccag
    10501 atacatcagc gacaagatgg tggtcatatc aatagtaacc agactgtgaa tcatggatgc
    10561 ggaaccatcg aaaatcagcg agacaacaaa gaagggaatg attgggatta aagcgctcca
    10621 gattaccagc gacatcaccg ccggacgcgt tgagtgcgac atgatctttt tattgaagat
    10681 gttgccacac gcccaactaa atgctgcagc cagggtcaac ataaagccga gcatcgccac
    10741 atgctgaccg ttcagactat cttcgattaa caccagtacg ccaaaaatcg ctaaggcgat
    10801 ccccgccaat tgtttgccat gcagtcgctc cccgaaagta aacgcgccaa gcacgatagt
    10861 aaaaaacgcc tgtgcctgta acaccagcga agccagtcca gcaggcatac caaagttaat
    10921 ggcgcagaac agaaaggcaa actgcgcaaa actgatggtt aatccatacc ccaagagcaa
    10981 attcagtggt actttcggtc gtgcaacaaa aaagatggcc ggaaaagcga ccagcataaa
    11041 gcgcaaaccg gccagcatca gcggtggcat gttatgaagc cccactttga tgaccacaaa
    11101 atttagcccc catacgacca ctaccagtag cgccaacacc ccatcttttc gcgacattct
    11161 accgcctctg aatttcatct tttgtaagca atcaacttag ctgaatttac ttttctttaa
    11221 cagttgattc gttagtcgcc ggttacgacg gcattaatgc gcaaataagt cgctatactt
    11281 cggatttttg ccatgcgtcc gatggttaat aacaacgacg aggcaagaag ggcgctggta
    11341 gagcgtcgta gggataagtt catttgtctg tccggcagtg cgtttcgtta tgttttattt
    11401 tagagatgta aagaaatagc atggctacgc tgccatttat gaccatttac ttgagtcgcc
    11461 agtacagcct gagtgtcgat ctaatcggtt atgcgatgac aattgcgctc actattggcg
    11521 tcgtttttag cctcggtttt ggtatcctgg cggataagtt cgacaagaaa cgctatatgt
    11581 tactggcaat taccgccttc gccagcggtt ttattgccat tcctttagtg aataacgtga
    11641 cgctggttgt gctctttttt gccctcatta actgcgccta ttctgttttt gctaccgtgc
    11701 tgaaagcctg gtttgccgac aatctttcgt ccaccagcaa aacgaaaatc ttctcaatca
    11761 actacaccat gctaaacatt ggctggacca tcggtccgcc gctcggcacg ctgttggtga
    11821 tgcagagcat caatctgccc ttttggctgg cagctatctg ttccgcgttt cccatgcttt
    11881 tcattcaaat atgggtaaag cgcagcgaga aaatcatcgc cacggaaaca ggcagtgtct
    11941 ggtcgccgaa agttttatta caagataaag cactgttgtg gtttacctgc tctggttttc
    12001 tggcttcttt tgtaagcggc gcatttgctt catgcatttc acaatatgtg atggtgattg
    12061 ctgatgggga ttttgccgaa aaggtggtcg cggttgttct tccggtgaat gctgccatgg
    12121 tggttacgtt gcaatattcc gtgggccgcc gacttaaccc agctaacatc cgcgcgctga
    12181 tgacagcagg caccctctgt ttcgtcatcg gactggtcgg ttttattttt tccggcaaca
    12241 acctgctatt gtggggtatg tcagctgcgg tatttactgt cggtgaaatc atttatgcgc
    12301 cgggcgagta tatgttgatt gaccatattg cgccgccagg aatgaaagcc agctattttt
    12361 ccgcccagtc tttaggctgg cttggtgccg cgattaaccc attagtgagt ggcgtagtgc
    12421 taaccagcct gccgccttcc tcgctgtttg tcatcttagc gttggtgatc attgctgcgt
    12481 gggtgctgat gttaaaaggg attcgtgcaa gaccgtgggg gcagcccgcg ctttgttgat
    12541 ttaagtcgaa cacaataaaa atttaattca gccttcgttt aggttatctc tgctaatatc
    12601 tttctcattg agatgaaaat taaggtaagc gaggaaacac accacaccat aaacggaggc
    12661 aaataatgct gggtaatatg aatgttttta tggccgtact gggaataatt ttattttctg
    12721 gttttctggc cgcgtatttc agccacaaat gggatgacta atgaacggag ataatccctc
    12781 acctaaccgg ccccttgtta cagttgtgta caaggggcct gatttttatg acggcgaaaa
    12841 aaaccgccag taaaccggcg gtgaatgctt gcatggatag atttgtgttt tgcttttacg
    12901 ctaacaggca ttttcctgca ctgataacgg atcgttgaca cagtagcatc agttttctca
//
LOCUS       DAOFMX010000104        12700 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.104, whole genome shotgun sequence.
ACCESSION   DAOFMX010000104 DAOFMX010000000
VERSION     DAOFMX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.104"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(104..742)
                     /gene="rutR"
                     /locus_tag="QBA20_003529"
     CDS             complement(104..742)
                     /gene="rutR"
                     /locus_tag="QBA20_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDQ6293933.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1030..2121
                     /gene="rutA"
                     /locus_tag="QBA20_003530"
     CDS             1030..2121
                     /gene="rutA"
                     /locus_tag="QBA20_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDQ6293934.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2121..2813
                     /gene="rutB"
                     /locus_tag="QBA20_003531"
     CDS             2121..2813
                     /gene="rutB"
                     /locus_tag="QBA20_003531"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDQ6293935.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2825..3211
                     /gene="rutC"
                     /locus_tag="QBA20_003532"
     CDS             2825..3211
                     /gene="rutC"
                     /locus_tag="QBA20_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDQ6293936.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3219..4019
                     /gene="rutD"
                     /locus_tag="QBA20_003533"
     CDS             3219..4019
                     /gene="rutD"
                     /locus_tag="QBA20_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDQ6293937.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4029..4619
                     /locus_tag="QBA20_003534"
     CDS             4029..4619
                     /locus_tag="QBA20_003534"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDQ6293938.1"
                     /translation="MNEAVNPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4630..5124
                     /gene="rutF"
                     /locus_tag="QBA20_003535"
     CDS             4630..5124
                     /gene="rutF"
                     /locus_tag="QBA20_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDQ6293939.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5145..6473
                     /gene="rutG"
                     /locus_tag="QBA20_003536"
     CDS             5145..6473
                     /gene="rutG"
                     /locus_tag="QBA20_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDQ6293940.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6555..6662)
                     /locus_tag="QBA20_003537"
     CDS             complement(6555..6662)
                     /locus_tag="QBA20_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293941.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7035..7631
                     /gene="wrbA"
                     /locus_tag="QBA20_003538"
     CDS             7035..7631
                     /gene="wrbA"
                     /locus_tag="QBA20_003538"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDQ6293942.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7652..7879
                     /locus_tag="QBA20_003539"
     CDS             7652..7879
                     /locus_tag="QBA20_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDQ6293943.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7917..9158)
                     /gene="agp"
                     /locus_tag="QBA20_003540"
     CDS             complement(7917..9158)
                     /gene="agp"
                     /locus_tag="QBA20_003540"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDQ6293944.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9451..10707)
                     /locus_tag="QBA20_003541"
     CDS             complement(9451..10707)
                     /locus_tag="QBA20_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HDQ6293945.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10968..11888
                     /gene="cbpA"
                     /locus_tag="QBA20_003542"
     CDS             10968..11888
                     /gene="cbpA"
                     /locus_tag="QBA20_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDQ6293946.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11888..12193
                     /gene="cbpM"
                     /locus_tag="QBA20_003543"
     CDS             11888..12193
                     /gene="cbpM"
                     /locus_tag="QBA20_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDQ6293947.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12286..>12700)
                     /gene="torD"
                     /locus_tag="QBA20_003544"
     CDS             complement(12286..>12700)
                     /gene="torD"
                     /locus_tag="QBA20_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDQ6293948.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3035 a         3246 c         3199 g         3220 t
ORIGIN      
        1 acgttcgctg agtgtgaata ccgctgccgc tggcggtaac gccagtttaa tgacgatcgg
       61 ttaaacaggt agccggagga tgttacaacc tcctccgtca tctttaacgt ggtcgaatcc
      121 cctcaataat aatccgctgc acgttttcaa ccgtttgatt gaaaaatacc tcatcgcgca
      181 acgtcgcgcc tgtcaccgcc tccacctgag gggcgaaatc ggcgtaatgt tgagtggaag
      241 cccaaatcat aaaaatcaaa tgctgcggat caatcggcgc gagtttgccg cttttgaccc
      301 aaccggcaat cagcgccgat ttctcatcaa ttaatgcctt caaatcgccc gtcagttcat
      361 ccattaacaa cggcgcgcct gccagcatct ccatacagaa caggcgcgaa gcctgcggat
      421 aatcgcgtga gacttccagc ttcagacgga tgtactcttt tatcgccgcc agcggggcga
      481 aatcttcacg aaacgctttt aacggtgcca gccagatatc gagaatctgc cgcagcacgg
      541 caatatacag cgcctctttt gacgggaagt aatacagcag attggttttt gaaacacccg
      601 ccaactctgc gatctgctcc agtcttgtgc cgtgaaaacc gaattgtgaa aaagtgtcca
      661 gtgctgcgct aagaatcgct tttttcttcg cgcttactgc gcgcgaacgt ttacccgttg
      721 ttttcactgc gccttgcgtc atgcgctctc ccctcttgta gattggtaac aggatagcaa
      781 aacccccgcc cggcctcgac cttctgcact ctcatcgcgc acggatgcat gttttatgtg
      841 caactgtttt gaccgtttag tccacttttt accagatatt ttagataaca caatgctaac
      901 acattaataa cattcacttt ttaaaaactg gcatccgctt tgcaaacaag ccaatacgcg
      961 gctgaaaagg atatgcagga tgcagcgccc cgtctgactt tcactttacg agacgaagag
     1021 aggttgatga tgaaaattgg cgtattcgta cctattggca acaacggctg gctcatttcg
     1081 acccacgcgc cgcagtacat gccgaccttt gaactgaata aagccattgc gcaaaaagcg
     1141 gagcattacc atttcgattt cgccctgtcg atgatcaaac tgcgtggctt tggcggcaaa
     1201 actgagttct gggatcacaa ccttgagtcg ttcaccttga tggcgggtct ggcagccgtg
     1261 acctcgcgca ttcagattta cgccactgct gccaccttaa cgttacctcc ggcaatcgtc
     1321 gcccgtatgg ccgcaaccat cgactccatc tctggcgggc gttttggcgt caacctcgtg
     1381 actggctggc aaaagcccga gtatgagcag atgggtatct ggcctggcga tgactatttc
     1441 tcccgtcgtt acgactatct caccgaatat gttcaggtgc tgcgcgacct gtggggcacg
     1501 gggaaaagcg attttaaagg cgattttttc accatgaatg attgtcgcgt cagtccgcaa
     1561 ccgagtatcc ccatgaaagt gatctgcgcc gggcaaagcg acgctggcat ggcgttctcc
     1621 gcccagtatg ccgatttcaa cttctgtttc ggcaaaggcg taaatacacc cacggctttc
     1681 gccccgaccg ctgcgcggat gaaacaggcc acagagcaaa ccggacgcga cgttggctct
     1741 tatgtgttgt ttatggtgat tgccgatgaa accgacgatg ccactcgcgc caaatgggaa
     1801 cactacaaag cgggcgcgga tgaagaggcg ttaagctggc taaccgaaca aagtcagaaa
     1861 gatacccgct ccggtactga caccaacgtt cgtcagatgg ccgatcccac ttcggcggta
     1921 aacatcaata tggggacgtt agtcggttct tacgccagtg tcgcgcgcat gttagatgaa
     1981 gtcgcaagcg tgcctggtgc agaaggcgtg ctgttgacct tcgatgattt tctgtcggga
     2041 atcgaaacct tcggcgagcg cattcaacca ctgatgcagt gccgcgccca tctctctgcg
     2101 ctgactcagg aggtggcatg atgacaacct taaccgctcg accggaagcc attaccttcg
     2161 atccgcagca aagtgcgcag atcgtggtgg atatgcaaaa cgcttatgcc acgccaggcg
     2221 gctacttaga tctcgccggg tttgatgtct caaccactcg cccggtcatt gccaacattc
     2281 aaaccgctgt gaccgcagcg cgagcggcag ggatgttgat catctggttt caaaatggct
     2341 gggatgaaca gtatgtcgag gctggcggcc ccggctcacc gaattttcat aaatcgaacg
     2401 ccctgaaaac catgcgtaag cagccgcagc tgcaggggaa attgctggcg aaaggctcct
     2461 gggattatca actggtggat gaactggtgc cgcagcctgg cgatattgtg ctgccgaagc
     2521 cgcgctacag cggtttcttc aatacgccgc tggacagcat tttacgcagc cgtggaatac
     2581 gccatctggt tttcaccggt atcgctacca acgtctgcgt cgaatcgacg ctacgcgatg
     2641 gcttttttct ggagcatttc ggcgtggtgc tggaagacgc aacacaccag gcggggccgg
     2701 aatttgcaca gaaagccgcg ttgttcaata tcgaaacctt ttttggctgg gtcagcgacg
     2761 tcgaaacgtt ctgcgacgcg ctttctccca cgtcctttgc tcgtatcgct taaggagttt
     2821 agcgatgcca aaatccgtaa ttattcccgc tggcagcagc gcaccgctgg cccccttcgt
     2881 tcccggcacg ctggctgatg gcgtggtgta tgtctccggt acgctggctt ttgatcaaca
     2941 taataacgtg ctgtttgccg atgacccaaa ggcgcaaacc cgccacgttc tggaaactat
     3001 ccgcaaggtg atcgagacgg cgggtggcac gatggcggat gtgaccttca acagcatctt
     3061 tattaccgac tggaaaaatt acgccgcgat taacgaaatc tacgccgagt tttttccggg
     3121 tgataaaccg gcgcgattct acattcagtg cggactggta aaacctgacg cgctggtgga
     3181 aatcgccaca attgcgcata tcgccaagtg aggccgcgat gaaactttca ctctcacctc
     3241 ccccttatgc tgatgcaccc gtagtggtgt tgatttcggg tcttggcggt agcggcagtt
     3301 actggttacc gcaactggcg gtgctggagc aggagtatca ggtagtctgt tacgaccagc
     3361 gcggcaccgg caataatccc gacacactgg cagaagatta cagtatcgcc cagatggcag
     3421 cggaactgca tcaggcgctg gtagccgcag ggattgagcg ttacgcggtg gtcggccatg
     3481 cgctcggtgc gctggtgggg atgcagctgg cgctggatta tcccgcgtcg gtaactatgc
     3541 tggtcagcgt taacggctgg ctacgaataa acgcccatac gcgccgctgt tttcaggttc
     3601 gcgaacgatt actgtatagc ggcggcgcgc aagcatgggt ggaagcgcag ccgttgttcc
     3661 tctatcccgc cgactggatg gcggcccgcg cacctcgcct ggaggcagaa gacgcgctgg
     3721 cactggcgca ttttcagggc aaaaataatt tactacgtcg acttaacgcc ctcaaacgcg
     3781 ctgactttag tcaccatgcg gatcgcatcc gctgcccggt gcaaatcatc tgcgccagtg
     3841 atgatctgct ggtgccaaca gcatgttcca gtgaacttca tgccgccctg cccgatagcc
     3901 agaaaatggt gatgccctat ggcggacacg cctgcaacgt gaccgatccc gaaacgttta
     3961 atgctctgtt actcaacggg cttgccagcc tgttacatca ccgtgaagcc gccctgtaag
     4021 gaattgctat gaacgaagcc gttaacccag gtgcgcttag caccctgttc accgatgccc
     4081 gcactcacaa cggctggcgg gagacacccg tcagcgatga gacgttacag gagctttatg
     4141 ccctgatgaa atgggggccg acatcagcta actgttctcc ggcacggatc gtgtttatcc
     4201 gcactgcaga aggaaaagaa cgtctgcgcc cggcgctttc cagcggcaat ctgcaaaaaa
     4261 ccctgaccgc gcccgtcacc gctatcgtcg cctgggacag tgaattttat gaacggttac
     4321 cactactgtt tccccacggt gatgcccgca gttggtttac ctccagccca caacttgccg
     4381 aagaaacagc gtttcgcaac agttccatgc aggcggctta tctgatcgtc gcttgccgtg
     4441 cgctgggact ggataccggc ccgatgtcgg gttttgaccg tcaacacgtt gacgacgcct
     4501 tttttgcggg cagcacgctg aagagcaatc tgctgattaa tatcggctat ggcgatagca
     4561 gcaaactttt tgcgcgcctg ccacgtctgt cctttgaaga agcctgcggg ctgttgtaag
     4621 gagcaaacta tgaacatagt tgatcaacaa acctttcgcg atgcgatgtc ctgtatgggc
     4681 gcggcggtca atatcatcac cacggacggt ccagccgggc gcgccgggtt caccgccagc
     4741 gccgtctgca gcgtgaccga cacgccgccc acattactgg tgtgcctgaa tcgtggggcg
     4801 tccgtctggc cggtattcaa tgaaaatcga acgctgtgtg taaatacact gagcgccggg
     4861 caggagccgc tttcaaatct ttttggcggc aaaacgccca tggaacaccg ctttgccgcc
     4921 gcccgctggc agacgggtgt gaccggatgc ccgcaactgg aagaggctct ggtttcgttt
     4981 gactgccgta tcagccaggt ggtgagcgtc ggcacccacg acattctgtt ttgcgccatc
     5041 gaagcgattc atcgtcacac cacaccctac gggctggtgt ggttcgatcg cagttatcac
     5101 gcgctgatgc gccctgcttg ttaacctcct taaggagaca gctgatggca atgttcggtt
     5161 ttcctcactg gcagttaaaa tcgacctcta cagagagcgg cgtggtcgcg ccggatgaac
     5221 gattgccgtt tgctcagacg gcagtaatgg gcgttcaaca cgcggtggcg atgtttggcg
     5281 cgacggtgtt aatgccgatt ctgatggggc tggatcccaa tctttccatt ttaatgtcgg
     5341 ggatcggcac gttactgttt ttctttatta ccggcgggcg cgtccccagt tatctcggct
     5401 ccagcgcggc ttttgtcggc gtggtgattg ccgcgacggg ctttaacggt cagggcatta
     5461 acccgaatat cagcatcgcg ctgggcggga ttatcgcctg tgggctggtt tataccgtca
     5521 ttggcctggt ggtgatgaaa attggtacac gctggattga acgactaatg ccgccggtgg
     5581 tgacgggtgc ggtggtgatg gcgattggcc tgaacttagc gcctatcgca gtgaaaagtg
     5641 tatcggcttc ggcctttgat agctggatgg cggtaatgac ggtgctgtgt attgggctgg
     5701 tggcggtgtt tacccgtggc atgatccagc ggttattgat tctcgtagga ttaattgtcg
     5761 cctgtctgct gtatggcgtg atgaccaacg ttttagggct gggaaaagcc gtcgatttta
     5821 ctctcgtcag ctatgctgcc tggttcggtc tgccgcattt ttccactcct ggttttaatg
     5881 gccaggcgat gatgttgatt gcgcccgtgg cggtgattct ggtggcggaa aacttaggtc
     5941 atctcaaggc ggtcgccggg atgaccggac gcaatatgga cccgtatatg gggcgagcat
     6001 tcgtcggtga tggtctggca acgatgcttt ccggctctgt cggcggtagc ggcgtcacca
     6061 cctatgctga aaatatcggt gtgatggccg tgacaaaagt ctactcgacg ctggtgtttg
     6121 ttgcagctgc cgtcatcgct atgctgttgg gcttttcgcc gaaatttggc gcgctgatcc
     6181 ataccattcc ggccgcggtg attggtggtg catcaattgt tgtgttcggg ctgattgccg
     6241 tcgcaggcgc aaggatatgg gtacaaaacc gtgtcgattt aagccagaac gctaatttga
     6301 ttatggtcgc agtaaccttc gtgctgggcg cgggtgattt tgctctcacg cttggcggtt
     6361 ttacgttggg agggatcggt acagcaacct ttggcgcgat tttactcaat gcgttattaa
     6421 gcagaaggtt ggttgacgtt ccaccgccag aagttgttca tcaggagcca taaacagaaa
     6481 accgccgggt taccggcggt gatgactttt tgacgagctt aagaaaaaaa tgcattcgtc
     6541 attgattagc ccggctagtt gtcgcttttg ccgtgactgc tcttgccacc ttttttacct
     6601 gcttctgatg ctctttcgcg gtcttctgca aaattgccgg aaccgcctcg atggtttgcc
     6661 attactgacc tccgtctggg tatagacatc atattgcata gaattaacgc ggcgtatggc
     6721 ggctgccata ttttcgctac gtgggactaa gcttagtgaa ctgacgcgga cagacccgcg
     6781 tttggtaata tcctgcaaca gcttcttagc gagaatagat atcatctcca ataattatcc
     6841 ataagccgct cttatgatta aaaaaacgcg atgagataaa aatgtgtcat tttgcgacaa
     6901 aattacgtgc ttgtgaaagt tgttataaat caaataagtg gttgtgaaat ttgcactctg
     6961 aaaaggacgt cttatcttta aataagtggt agcgaatcgc tacggaatag agataacacg
     7021 aggagtggtt agaaatggct aaagttctgg tgctttatta ttccatgtac ggacatattg
     7081 aaacgatggc acgcgcagtc gctgagggtg caagcaaagt ggatggcgca gaagttgtcg
     7141 ttaagcgtgt accggaaacc atgccgccgc aattatttga aaaagcaggc ggtaaaacgc
     7201 aaactgcacc ggttgcaacc ccgcaagaac tggccgatta cgacgccatt atttttggta
     7261 cacctacccg ctttggcaac atgtccggtc aaatgcgtac cttcctcgac cagacgggcg
     7321 gcctgtgggc ttccggcgca ctatacggaa aactggcgag cgtctttagt tccaccggta
     7381 ctggcggcgg tcaggaacaa accatcacat ccacctggac gacccttgcg catcacggca
     7441 tggtgattgt ccccattggc tacgcagcgc aggaattatt tgacgtttca caggttcgcg
     7501 gcggtacgcc gtacggcgca accaccatcg caggcggtga cggctcacgc cagccaagcc
     7561 aggaagaact gtctattgct cgttatcaag gggaatatgt cgcaggtctg gcagttaaac
     7621 ttaacggcta atcttcaaca ggaggatacg catgccaact caagaagcga aagctcatca
     7681 cgtcggtgaa tgggcatctc tgcgcaatac gtcgccggaa atagccgagg ccattttcga
     7741 agtcgccggg tacgacgaaa aaatggcaga aaagatttgg gaagaaggta gtgatgaagt
     7801 gttagtcaaa gcctttgcta agaccgataa agattcgctt ttttggggcg aacagaccat
     7861 cgaacgtaaa aacgtttaac cagcgactcc cccgcttctc gcgggggagt tttctgttat
     7921 ttcaccgcct cattcaacac gctatcaaac ttatccatcg ggcagaaacc gttggcatct
     7981 atcgggcaac cgcttaattc cagcgtcacg cgctgcgcag gtgcctgcag ggttaacgca
     8041 tcggcattac gtaactgttc cgcactctgg tacacatatt caattttcat caaatcgcga
     8101 ttggctttgc tgtcatgcca acgctggaaa acgattttgc cgccaatcgg cgtgcgttcg
     8161 ttctggtcat gcaactgata cggtttgaaa tccagcgccg ttaacagaga ggctatgttg
     8221 gagtcgtgcc caactaacac cgtgattttc ggagcactgg cgcgatcggt gaccagggct
     8281 ttgtcgatat aactgaccag cggtttcgca acattgcgcg ccacttccgg tgaggtaaac
     8341 agactgtcct ggtaaccgtt tttcagcttc gacaatacct tccactgctg gtcagatttg
     8401 atttctcccc aggccacctg atccatcgga aaaccttcgt agtattgcaa agtaaacgca
     8461 tctaccagcg agttgccgac tttcagcggc ccggaaacac ctggttcttg ttgatacttg
     8521 gcgctaaagg tatttttgcc atccaccagc gaacactgtt gtttctcttt acaggcaggg
     8581 gaatctttat agttaacgat tttttccagt agctggtagc tgtcggtaag ctggagtttg
     8641 ctgagctctt tctccattgc cgccaccgcc tgttcactga atgcggcgga atcatcggtg
     8701 atcatcgggt taaaggttgg gtccatagtg cccatttttt cctggtgatg cacaggaata
     8761 tcacacccag ggaatgcacc agtaataaag aactgggcgg tagcgacggt acgttgcaga
     8821 ctattggcat aggcataaac ggtgtccggc ggcgggcatt cccccgattt cactatcccc
     8881 tgctctgcca gccattcacg catgtaatgg cccatataca cttcgagcac gccacctttg
     8941 gtggtgagtt gcccaccggg gacgtcccat tcgggccatt tattcggcgt cgactgctcc
     9001 agcacactgc cattgttcgc cagcggcgca cgtaagttat ggcggctcat catgagcact
     9061 tgctgtagct gatagccttc cggtacggtt tgtgcctgag catttgaagc gagtaaaact
     9121 atccctgcca cagttgcggc gattagcgtt ttgttcattc ctggcacctc ttttgttatc
     9181 aatcattaaa gagtgtgaca gaaatatgac ttttttccgg gaacaggttc gcaaaatggc
     9241 ttgttgaacg cctgtttttc tatatacatc ttcaaaaaca ggcaaggttt gacgcaaact
     9301 atgcctgatt agcccctgcc tcgcgtacgc cgctgataat tgcacctgtg gctaaacaga
     9361 atatctgcgg cgttaccaat acagagacaa tatttaccaa taaaaaagtg tgagactaat
     9421 aacaatggtt tttagcatat gcctttgcca ttaagttgca taacgattta aatagtcaag
     9481 ttgcgcattc atttccttat tttttaactt ataagcctga gaaaaaagtt caggagggag
     9541 gtaaacatca taatgattgc cagtgtttgt taaataacca tactcaagat cttcatctgt
     9601 tgaaaaggat tcatttccca aggagattga tgccacatta ctattaacaa taaataatga
     9661 gcaaatgcta tctgaaagaa cagcaagaaa attcttcacc gctttaatcc tatccgacgt
     9721 gtcggtatct aaagcaagca aattcttgtt atcattaaat ataaactcgg gcaaatgcag
     9781 tatgcatttt ttgtcattca agcaactatc aatgaattgt tttttaaaaa acagaaagct
     9841 ccatgacata aaactaccca caccctgaat tttgttgtca gattgcagat caacgccaaa
     9901 taatgtcttt acatcttccc tgagattata gaatgccgta tcaacaagag cctcgatcgt
     9961 ttgtttatca acaattttaa taaactcttc gtatacttgc ttttgcgtct tatggctgcc
    10021 cagcgcccat gaatactcat gttcttttaa aaccagtatg gctcttaaca gacaatatcc
    10081 atcgccatct actggagcgt attttaatcc atatgtttta ttaatttcac tctgaataaa
    10141 agatatgcca gacaaatcaa taccagtatc taaatcagat attcccggca tatttatctg
    10201 ttctggctgg atactcatat tttcatcatc taaacaatac tcagaaaaaa catcatcaca
    10261 cgcattaatg ttttcaatat attccataaa gccataccaa taataacctt taaaatctgt
    10321 gatagactct gaacctctgc ccggcaagga cactctaaca atatcaacat cttgaacata
    10381 ttgtatagta tatgtaatac ctcctgaaac ccctgaataa atttgtgtat cattagtacg
    10441 caatgttctc ataaataatg atagatcatt atcattctgc atttcctcaa attctttttc
    10501 tagtgattca cattccagac atggagtatc ctcttcattt actttacgtc tgagttcatt
    10561 gtacaaccgc ataaaacaca ccagtgtgtt ttcaaaaagc gtgattgaat atgtttgatt
    10621 tttttcgtac tcatttttta tatcgttcca ggcctgtttc aaataattat ttgcagtgca
    10681 actaattcca tgtatattac tacccatata tagcgtctat aaaatttaat aaataatgac
    10741 gccctagtta aacttaaagt gcctggttca actatcaaaa atcgctcacc ctttttcacc
    10801 tgtttaaaat atgttcagca acccatcttg atggcgacct cctctccgcg atgatttcaa
    10861 taacatattc tgtgttggca tatgaaattt tgaggattac cctacactta taggagttac
    10921 cttacagggg ttccttcaat ttgtgttgat ttacgcgaga taacgctatg gaattaaagg
    10981 attattacgc catcatgggc gtgaaaccga cggacgatct caagacaatc aagaccgcct
    11041 atcgtcgact ggcccgtaaa taccatcctg atgtcagcaa agaaccggat gccgaagccc
    11101 gcttcaaaga ggtcgctgaa gcctgggaag tgttaagtga tgaacaacgt cgcgctgagt
    11161 atgatcagat gtggcaacat cgcaacgatc cgcaatttaa ccgtcagttc catcatggcg
    11221 acggtcagag ttttaatgcc gaagattttg acgatatctt ctcgtcaatt ttcggtcagc
    11281 atgcccgcca gagccgtcaa cgccccgccg cacgcggcca cgatattgaa atcgaagtgg
    11341 cggtattcct cgaagaaacg cttactgagc ataagcgtac catcagctat aacctgccgg
    11401 tttataacgc ctttggcatg atcgaacagg aaatcccgaa aacgctgaat gtgaagatcc
    11461 cggcaggcgt cggcaatggt caacgcatcc gcctgaaagg ccaggggacg ccgggcgaaa
    11521 acggcggtcc aaatggcgat ttgtggctgg tgattcatat tgcgccacat ccgctgttcg
    11581 atattgtcgg ccaggatctg gaaattgtgg tgccggttag cccgtgggaa gcggcgctgg
    11641 gtactaaagt caccgttcca acactgaaag aaagcatttt gctgactatc ccgccaggca
    11701 gccaggccgg gcaacgattg cgcgttaaag gcaaaggtct ggtgagcaaa aaacagaccg
    11761 gcgatctgta tgcggtactg aaaatcgtga tgccgccgaa accggatgaa aacactgccg
    11821 cgctgtggca gcaactggca gacgcccagt cgtcttttga tccacgtaaa gattggggga
    11881 aagcataatg gctaatgtta cggtgacttt tactattacc gaattttgcc tgcataccgg
    11941 catctctgaa gaggagttga atgaaattgt cggtttgggg gtggttgaac cgcgtgagat
    12001 ccaggaaaca acctgggtat ttgacgacca tgccgccatt gtggtgcaac gcgcggtacg
    12061 cctgcgtcat gaactggctc tggactggcc ggggatcgcg gtggcgctga cgttaatgga
    12121 tgatattgcg cacctgaagc aggaaaaccg cctgctacgc cagcggcttt cccggtttgt
    12181 agctcatccg tgatgtgtgt ttgccggatg tgatgacgca tccggcaact gcgccataat
    12241 gcctgatgcg acgctaaacg cgtcttttca ggcgcataaa cgacgttatc tgttttggtg
    12301 gtcgcactcc actaacacca gcaataactg gcttagtgcc gcgtaaaaac caaagctgtc
    12361 atactgtcga caacgcgcag caaactctgg taaccattgc cgcagcgccg tcagtgtttt
    12421 ttgccgcaaa ctgtcgattc ttcgcgcagg aacggtcccc tctcccagcg aaaaatgcag
    12481 ataactcaac aactcgagat agatcgccag atgatctgcc ggttcgttga aattgccact
    12541 ggtttccatc cctgcctcaa ctaacaagcg tttaatctct tgctcgtcct gtttgtaggc
    12601 cgatgcatac ggcagcgccg cttgtttgtc ggtcatcaga aacaggccgc aaaagtcagc
    12661 ggccagttcc agacgggcat cgtcacgtac tgtcagcgtg
//
LOCUS       DAOFMX010000105        12662 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.105, whole genome shotgun sequence.
ACCESSION   DAOFMX010000105 DAOFMX010000000
VERSION     DAOFMX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.105"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="QBA20_003545"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="QBA20_003545"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDQ6293949.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="QBA20_003546"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="QBA20_003546"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDQ6293950.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASMAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKLVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="QBA20_003547"
     CDS             3115..3399
                     /locus_tag="QBA20_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDQ6293951.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="QBA20_003548"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="QBA20_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDQ6293952.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="QBA20_003549"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="QBA20_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDQ6293953.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="QBA20_003550"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="QBA20_003550"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDQ6293954.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="QBA20_003551"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="QBA20_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDQ6293955.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9585..10685
                     /gene="ompC"
                     /locus_tag="QBA20_003552"
     CDS             9585..10685
                     /gene="ompC"
                     /locus_tag="QBA20_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDQ6293956.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10849..11334
                     /locus_tag="QBA20_003553"
     CDS             10849..11334
                     /locus_tag="QBA20_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDQ6293957.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11444..12433
                     /locus_tag="QBA20_003554"
     CDS             11444..12433
                     /locus_tag="QBA20_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDQ6293958.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3335 a         3129 c         2945 g         3253 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagcggc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag catggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa actggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tggcaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc gacttcgatc acatatcgat aaataatatt attctgtttt tctcattgtt
     9481 tttaatcgcc attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt
     9541 cgtttagaaa atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag
     9601 ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata
     9661 acaaggatgg taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg
     9721 atgataagcg tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc
     9781 aaatcaatga tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg
     9841 atgaagccaa cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta
     9901 gtgaatttgg ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat
     9961 ggaccgatat gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga
    10021 cataccgtac caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag
    10081 ggctgaactt tgccgcgcaa tatcaaggca aaaatgaacg tactgacaac agtcatcttt
    10141 atggtgctga ctacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt
    10201 atgatggctt tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa
    10261 gagccgcagc taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta
    10321 taaagtatga tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga
    10381 ccacctatgg ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat
    10441 atcaattcga cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc
    10501 tgggccgcta cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct
    10561 tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact
    10621 ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt
    10681 tctaacgtcc tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt
    10741 atgactcctt aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat
    10801 attattccat atcctccggg gacatcttac tataatattt attgatgagt gattatgacc
    10861 aatattaata cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct
    10921 aacaaagaaa ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg
    10981 gttaataatg gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg
    11041 aacatggcat ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg
    11101 acagttaata cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat
    11161 gccagtctcg ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa
    11221 gaaccaaaac tggaaaacat ttatattgat cataacaatc tggaaagttt agatttaaaa
    11281 aatcagcatt cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc
    11341 tgatagttat aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt
    11401 ctcccggcaa gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat
    11461 attacacaat catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt
    11521 aaacgccaaa aacactacaa aactggaata tttaaactta agcaataaca atttactgcc
    11581 aaccaatgac attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg
    11641 catcaacaat gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga
    11701 tgacactaat gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg
    11761 gctgcaaaac ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac
    11821 caactacgat agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt
    11881 tcctcaaggc agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa
    11941 tattgatata gataggcttt catcagttac ttattttagt gcggcacata atcaactaga
    12001 gtttgtgcaa ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt
    12061 aactgatatt gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa
    12121 actaacaagt ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa
    12181 tttgctttct gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc
    12241 tgctaacaac cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa
    12301 aagtttaaga ctggacaata ataaaataac ccacattgat actaataata catccgatat
    12361 tggaacttta ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg
    12421 gaagaacaat tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa
    12481 gcttaatact ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat
    12541 agatgcggat aacgaattgt ttcactatac tattaaaaaa gcatataccc catataatat
    12601 acacacttac aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac
    12661 ct
//
LOCUS       DAOFMX010000106        12564 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.106, whole genome shotgun sequence.
ACCESSION   DAOFMX010000106 DAOFMX010000000
VERSION     DAOFMX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.106"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..1038
                     /locus_tag="QBA20_003555"
     CDS             <1..1038
                     /locus_tag="QBA20_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDQ6293959.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1039..3204
                     /locus_tag="QBA20_003556"
                     /pseudo
     CDS             1039..3204
                     /locus_tag="QBA20_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
     gene            3218..4129
                     /locus_tag="QBA20_003557"
     CDS             3218..4129
                     /locus_tag="QBA20_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HDQ6293960.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VLKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4129..5424
                     /locus_tag="QBA20_003558"
     CDS             4129..5424
                     /locus_tag="QBA20_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HDQ6293961.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5469..5699
                     /locus_tag="QBA20_003559"
     CDS             5469..5699
                     /locus_tag="QBA20_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293962.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5677..6177
                     /locus_tag="QBA20_003560"
     CDS             5677..6177
                     /locus_tag="QBA20_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HDQ6293963.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLIATARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6177..7595
                     /locus_tag="QBA20_003561"
     CDS             6177..7595
                     /locus_tag="QBA20_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HDQ6293964.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIVSASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7595..8443
                     /locus_tag="QBA20_003562"
     CDS             7595..8443
                     /locus_tag="QBA20_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6293965.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8443..8898
                     /locus_tag="QBA20_003563"
     CDS             8443..8898
                     /locus_tag="QBA20_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HDQ6293966.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSGTREELIDFLNHGR"
     gene            8901..9593
                     /locus_tag="QBA20_003564"
     CDS             8901..9593
                     /locus_tag="QBA20_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HDQ6293967.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9603..10934
                     /locus_tag="QBA20_003565"
                     /pseudo
     CDS             9603..10934
                     /locus_tag="QBA20_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
     gene            10935..>12564
                     /locus_tag="QBA20_003566"
     CDS             10935..>12564
                     /locus_tag="QBA20_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDQ6293968.1"
                     /translation="MVNYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPRTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDALQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQAPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAK"
BASE COUNT         3374 a         3107 c         3315 g         2768 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgcagact ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt
     1141 agccgagtgt cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga
     1201 caattcgatg ttgttcgccc ggggggcagg aaactggtcg cagagatgcg ccggaaccct
     1261 gtcgacgttc tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg
     1321 gggatgtatc gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt
     1381 cgtgagcaga tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac
     1441 gatccaacaa gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac
     1501 gtcatatgag acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg
     1561 attaacgcct tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg
     1621 gataccctgc catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat
     1681 gatgtcgtct acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc
     1741 taccgcgacc cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc
     1801 atcgacgacc tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg
     1861 cgtgtgtata agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc
     1921 ggagagcata ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag
     1981 tgctacgagg gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg
     2041 tcgttcaacg ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag
     2101 caaatcgaag gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag
     2161 aacaggaccg atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac
     2221 cctgaagtgc cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa
     2281 gtggctagtc ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc
     2341 aatcaactga acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc
     2401 gcaatgcgac gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct
     2461 cgtcatgtaa cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa
     2521 gttgtcgatt accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag
     2581 tgctatacag acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt
     2641 caggagttgc tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa
     2701 tacttcacgc tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa
     2761 ctggtgatga tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag
     2821 gcacaacaac agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg
     2881 caaggtcagg ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct
     2941 gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat
     3001 atggacctcg acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag
     3061 caacagcgca gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga tgcagaccag
     3121 actcattcac aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc
     3181 ggagtagccg agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca
     3241 ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt
     3301 tgtcgataat gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tcctgaaaga
     3361 cgatgagaaa ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga
     3421 acgcaaacgc cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc
     3481 ggagcacctg cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc
     3541 cgaagatgca ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca
     3601 ggcaaacagt gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca
     3661 gggtcgcaaa actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa
     3721 gcactacgag gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt
     3781 catgcaactg gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc
     3841 cgccgctctc atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat
     3901 ggacgggcaa tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc
     3961 tcgagccaag cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt
     4021 tgctgcaaat atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt
     4081 agagacgtac cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat
     4141 gaaggtcaac tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg
     4201 ccaatggcgt caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc
     4261 aacaccgtgt ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact
     4321 ggcaacgcaa ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac
     4381 gatttcttcg agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc
     4441 caggcatccg ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact
     4501 gaaatgggct cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct
     4561 ggctgggatt ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac
     4621 atgggcatca gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta
     4681 gatggtgaca tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt
     4741 cagattgctg gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca
     4801 accgcgactg gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt
     4861 gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc
     4921 accaccggat ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag
     4981 atggcgaaga atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt
     5041 actcacatcg aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa
     5101 gagaaggctt acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg
     5161 aacgtggcaa caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct
     5221 cagccgatcc cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt
     5281 ccaggtattg gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt
     5341 aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt
     5401 ggtctgccta accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg
     5461 agacaagcat gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg
     5521 gctacgactt tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt
     5581 tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc
     5641 gtaaaaagcc tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat
     5701 ttagttcttg cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc
     5761 gaaccgcagt ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt
     5821 ggcggtgatg cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct
     5881 ccggatccgg gtgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac
     5941 cttgcctgcc gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tatagccact
     6001 gccagatacg ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct
     6061 aaatgtaagt ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag
     6121 tggaatggtt ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc
     6181 ctattcagca acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata
     6241 tcgactatct gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat
     6301 atcttcgctc attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg
     6361 tcgagtacaa catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag
     6421 gagaaagtga agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga
     6481 catcacaggc ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca
     6541 aaaccgtctc aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc
     6601 gcgacattac gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt
     6661 ttatcactga ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg
     6721 agtctcagcc tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg
     6781 gttcatcgac tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt
     6841 atgttgcaca accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc
     6901 cattcgctga ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata
     6961 tcatcgactc cggtcaggtg tcaccaattg tcagcgcgag cattgagaaa atcctccgct
     7021 cctacactgc tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg
     7081 agttgctgat tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaagcgcca
     7141 atggcccaca atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg
     7201 acttcgttta cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga
     7261 aactgcaatt cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc
     7321 cactcttcaa agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg
     7381 ttgcgcagta cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc
     7441 gtgagcagat gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc
     7501 gagtagggcg catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg
     7561 tcactctgtc tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc
     7621 ggtaatcatc caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca
     7681 gtcgtatctg ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc
     7741 caacgaggcc ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat
     7801 tctcgctgac catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga
     7861 gcgaatcaga caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga
     7921 aagcgatatt tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca
     7981 tgaaacgcgc atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta
     8041 ctctggtgta tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac
     8101 gctcacaccg tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt
     8161 tgtgttcaac gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag
     8221 cggaaccgcc aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt
     8281 cagcagtcgt aatgcggcga caacaaccga caacatcctg ttagctacgt tcttcagcgt
     8341 ggataaagac ggctttcttg ccacaaatgg cagtacgtta accattcagt cgaatggtgc
     8401 ggcgtttact gccacaacca tcaagataat cgcggagcag tgatgattac attcaaacca
     8461 acgcgaaaca tcgacctgat agaagcagtc ggaaatcacc ctgacattat cgccgggagc
     8521 aacaacggtg atggatacga ctacaaacct gaatgccgtt actttgaggt gaacgtgcac
     8581 ggtcagtttg gcggcattgt ttactatcag gagattcagc cgctgacatt agattgccac
     8641 gccatgtacc tgccagagat tcgcggcttc agcaaggaaa ttgggctggc gttctggcga
     8701 tacattctga ctaacaccac cgttcagtgc gtaacatcgt tcgctgcacg caaattccga
     8761 cacggtcaga tgtactgcgc aatgattggc cttaagcgtg taggaaccat caagaaatac
     8821 ttcaaaggcg tggatgacgt gactttttac agcggcacac gcgaagaact aatcgacttc
     8881 ctgaatcacg ggagatagcc atgttatatg catttaagct gggcagaaaa ctgcgcggcg
     8941 aggaacctta ttgccctgaa aaaggcggga aaggtggcag ttctgataaa agcgcaaagt
     9001 atgcagcaga agcccagaag tatgctgcag acctgcaaaa tcagcagtgg cagacgatca
     9061 tgaaaaacct tgctccgttc acgcctcttg cggagcagta tgttaaccag cttcagaacc
     9121 tttccagttt agaaggtcag gggcaggcac ttaatcagta ttacaactct aagcagtata
     9181 aagaccttgc agggcaggcg cgttaccaga gtcttgctgc tgcggaggcg acgggtggac
     9241 ttggttcgac agccacaagc aatcaactgg ctacgatcgc gccgacactc ggtcagtctt
     9301 ggttatcaaa tcagatgagc aattacaaca atctggcaaa cgttgggctt ggtgcgctgc
     9361 aaggtcaggc aaacgccggg cagacgtacg ccaacaacat gagcagcatt gcacagcaaa
     9421 gcgcagctct tgccgctgct aatgccaata aaccatcaag tcttcagact gcaattagcg
     9481 gtggcacgtc tggtgcgatt gccggtgcag gtcttgccag ccttttggga acatcaacgc
     9541 cttggggcgc tggtattggt gctggtatcg gattgcttgg ctcgttgttt taaggggtaa
     9601 tcatggctac ttggcaagga acaaacggcg gattgttggc tggtatcggc ggcgtcaact
     9661 caaacgctcc gagcgtaaat gacatcggca atacgcttca gcttatcagg cagaacaatg
     9721 atattgagcg ttcaggcgct aacaatgttg ggctgactgc tttgcaaggt ctttcaggta
     9781 ttgcgggtgt ttttcagcag gaaaagcagg ctcagcggca gaaagaattt cagcaggcgt
     9841 acgctaatgc ttatgcgtct ggtgatcgcg gtgctttgcg tcagttggct actcaatatc
     9901 cagaccagat tgaatccgtt cgtaaaggca tgggattcat tgatgaagag cagcgtaatt
     9961 ctatcggcac cttagcggct ggcgcacgcc ttgcgtcatc gtctccagaa gcaatgcaat
    10021 catggctgca aaacaacgcc ggtgagttag ctcgtgttgg cgttaatcct catgacgtcg
    10081 ctcagatgta ccaacagaac ccgcggcagt tcggcgaatt tgtcgatcac ctggggatga
    10141 acagtctcgg gcccgaaaaa tactttgacc tacaggataa aatgcagggt cgccaggtta
    10201 ccatgcgcgg tcaggatctg gattcgcaaa ccgccgctcg gaatcaggca atcacaatgc
    10261 gcggacaaga tatccaggcg aatttaggtc agcagcgcat taatctggac gcagaaacaa
    10321 accgcattaa caacgaaaat aagcgccttg accggatgct atcagcagaa actaacgacc
    10381 tgaagcgcca ggaaatacag agccgcatag cagccaacaa ccagcagttg cagcagaagc
    10441 agcaagcgct aaatgatggc tacaaagacg gcatcaacac cctcacaacc agcatgttca
    10501 ctctgaacga tatcgttagt tctccttcac ttaagagcat tacaggctta cgtggagtaa
    10561 tccccaacgt tccaggctca caggctgcag acactcaggc acgacttgat acctttaaat
    10621 cacaagcata tctgacagcg gttcaggcca tgcgaggcat gggcgcactt tctgatgccg
    10681 agggcaaaaa gctcgactag gctgttggtt cgctgcagaa ctcgcagagc gaggagtcct
    10741 ttcgtcgcaa cgctggcgtc atcctgaaca cgctcaacca gaagcgtaat gaggcggttg
    10801 gtaagtacgt tcagcaaaac ggtatcaagc gagtggaagc gcctcaggct tctatagatt
    10861 acctgaagca gcaccccgag ctgtcaatcg acttcattaa tcgctacgga tatcttccat
    10921 ctttggggca gtaaatggtt aattaccgtg atttgttaga gcaggctggc gcacgttacg
    10981 gtgtgccaga agggttgatg actgcactgg gtgccaagga gtcttcttac aaccctgccg
    11041 cagtaagctc cgccggggct gtaggattga ctcaggtcat gcctaggaca tggcgtgata
    11101 tgggttatac cgatgagcaa atgcaaaacc ccgaatatca ggctgacgct ggcgcgcgct
    11161 atctggcaaa gatgtaccag cagtttggta actggcgtga cgctcttcag gcttatcacg
    11221 acggtcccgg caacgttatg aaggcaaagc gtggtgaata tacgccagga cctgaaggcc
    11281 gcggttacgt tgatgatcgc tttgctcaat gggctggtga cccggtgaca gactcaacag
    11341 tcgaacagcg cgccacctct gcaaaggtac atcctcagca agcccctaac aacccgtttg
    11401 cacaactgga agtacagtca tccgaacaag tatcggcatc aggtgtgcag tcagacccaa
    11461 ataatccatt tgctcagatt gagcagcagg cagccagtca gcagccacct caacccgtaa
    11521 gttctgtcgc accgaaacct gttcagcaac aaacagttaa tcaggccaat aatgcaccaa
    11581 cacgggaaga accatcattg atgcaacaag ctggcgattg gctcactggt ggtcaaagtg
    11641 cagggcaaat tgcagaacag gctggtcgtg gtctggtaaa cataccattt gacgtattgc
    11701 agggtggcgc aagtctgatt aatgcaatca gtcaggggct tggggggcca aaagtattgg
    11761 atgatgttta tcgtccagta gacagaccga cagaccccta cgcgcaagct ggagagtcaa
    11821 taggcggtta tcttgttcct ggagcaggtg tagctggaaa catggtcatt ggttctctcg
    11881 ctgacgcggc gaatcaacgg ggtgattttg ccgaaaatgc cgctattaat gccggactta
    11941 gcattgctac gcatggcctg ataaatggcg ttacccgtgg tgttcgtggt gcatcaaata
    12001 taattagtgg caataaaaca tctgcacaga gagcgaccac tgcgccaaca gaaacatcac
    12061 cattctccgg tgatgccgct gcagcaacaa atcctgcggt tcatgccgca gaggcaagag
    12121 tagcacaagg tgtaccaatg acgcctgcga cgaagaaccc agaggaagtc gttcgcacag
    12181 tagcagcaca aaaaaggcca aatctcgctt catcgcttga tgaactagat atcaatcctc
    12241 aggctgaagt tctggagtct gctgaaaggc ttaatgttga ttcattactc ccttcacact
    12301 tttccgggaa cgagcaatac aaggcagttg agcaagcaat caagtcccgt gcgggttctg
    12361 ctctacaggt gcaggaaaat gaagcaatca ggcagctagc acagggcgcg ggtgagataa
    12421 ttgatcgcgt ttccggtgca aaagatgctc ttggtatgag cgacaagttt attgatacgg
    12481 tcaatggaag aatgtctgcg ctgatgaaac gaagcgacca gctttatcgc aatgttgaaa
    12541 aggcgatgcc tgcaggtgca aaaa
//
LOCUS       DAOFMX010000107        12262 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.107, whole genome shotgun sequence.
ACCESSION   DAOFMX010000107 DAOFMX010000000
VERSION     DAOFMX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.107"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..98
                     /locus_tag="QBA20_003567"
     CDS             <1..98
                     /locus_tag="QBA20_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6293969.1"
                     /translation="FEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            95..496
                     /locus_tag="QBA20_003568"
     CDS             95..496
                     /locus_tag="QBA20_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6293970.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            507..1247
                     /locus_tag="QBA20_003569"
     CDS             507..1247
                     /locus_tag="QBA20_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDQ6293971.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1306..1692
                     /locus_tag="QBA20_003570"
     CDS             1306..1692
                     /locus_tag="QBA20_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDQ6293972.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1701..2030
                     /locus_tag="QBA20_003571"
     CDS             1701..2030
                     /locus_tag="QBA20_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDQ6293973.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2002..5043
                     /locus_tag="QBA20_003572"
     CDS             2002..5043
                     /locus_tag="QBA20_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDQ6293974.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5043..5372
                     /locus_tag="QBA20_003573"
     CDS             5043..5372
                     /locus_tag="QBA20_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6293975.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5372..6070
                     /locus_tag="QBA20_003574"
     CDS             5372..6070
                     /locus_tag="QBA20_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDQ6293976.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6075..6818
                     /locus_tag="QBA20_003575"
     CDS             6075..6818
                     /locus_tag="QBA20_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6293977.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6755..7357
                     /locus_tag="QBA20_003576"
     CDS             6755..7357
                     /locus_tag="QBA20_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDQ6293978.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7418..10897
                     /locus_tag="QBA20_003577"
     CDS             7418..10897
                     /locus_tag="QBA20_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDQ6293979.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWITASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10965..11564
                     /locus_tag="QBA20_003578"
     CDS             10965..11564
                     /locus_tag="QBA20_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDQ6293980.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11628..>12262
                     /locus_tag="QBA20_003579"
     CDS             11628..>12262
                     /locus_tag="QBA20_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDQ6293981.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         2888 a         2915 c         3922 g         2537 t
ORIGIN      
        1 catttgaaga tgcccgcgac cgcatcattg ctgcggaaat gccgaaacag ctggggtatg
       61 ccctgaaaca acaactgagg ttacatctga gtaaatgaac cgacacacac aaatccgtca
      121 gagcgtgctg gcacgccttc gggaacagtg tggagacagc gccacgtttt ttgacgggct
      181 tccggcattt attgatgcgc aggaactgcc tgccgtggcg gtgtggctga gtgatgctca
      241 gtacaccgga aaaatgacgg atgaagatga ctggcaggct gttctgcata ttgccgtctt
      301 catccgggca caggcaccgg attcagagct ggatatgtgg atggagagca ccattttccc
      361 ggccctgaat gatgtaccgg cactttccgg actcatcgac accctgaacc cactcggttt
      421 taactatcaa cgtgataatg agatggctac ctgggcgatg gcggaaatca cgtaccagat
      481 cacgtacacg aattaaggag gtggtaatga ccacaccaaa tccactggca aagacgaaag
      541 gtgcggggac gacgttctgg atgtataccg gcaacggcga tgcgtttgcg aaccctttgt
      601 cggacactga ctggctgcgt cttgcgatgg tgaaggatct gcaacctggc gaaatgaccg
      661 ctgatgcaga agatgacact tatctcgatg atgaagatgc agactggaaa acgacaaccc
      721 aggggcagaa atccgttggt gatacttcgg cgacgctggc ctggcgtccg ggtgacagcg
      781 ggcagaaaaa actggttcag ttgttcgact ccggtgaagt ctgcgcgttt cgtatcaaat
      841 atcccaacgg cactgttgat gttttccgcg gctggctgag ctcactgggt aaaaccattg
      901 cctcaaaaga cgtgatgacc cgcactgtga aaatcagcgg tgtggggcgt ccgtatctgg
      961 cagaggaagg cactgaaacc gtgggtgtta ccggactgac ggtggcaccg gcatctgtca
     1021 gtgtcaaagc gggagcaacc accacactga cctttacagt aaaacctgac ggggccagtg
     1081 acaaagcgat cagtgtgcat tcgtcagatc ctcagactgc ttcggtgacc ctgagcgggc
     1141 ttgtggccac ggtgaaaggc gtgaagcagg gcagtgtcag catcgtgggc atgacctctg
     1201 acggagagtt tgtggcagtg gctgcggtga ccgtcagcgc accataacag gacgatactc
     1261 atcattgccc cggttatccg gggctttttg catccggaga acatgatgtt tctgaaacag
     1321 gatacgttta attatgaaaa acagtccgtg gtgctcagtg agctgtccgg gctgcagaga
     1381 attgaatatc tgacgtttgt tcagcagcga acggcaaagt ttgatgcaca ggagggagaa
     1441 ctgccggagg ctgaacgaca gattgctttt ctgcgtatgg gaatggatat caatgcctgg
     1501 ctggtttccc gctcactgtg gaatgctgag cagtctcagg atgttgagac gctttgcgca
     1561 tccattatga caacatggtc gtatgatgcg ctgggcgcgg gggcggagag ggttctgtcg
     1621 ctgagcggta tggggaccat tgagaatgcc ggggatgatg atcatgaggc gctgacgccg
     1681 gaaaagtcct gacgcgggaa atgcagtttg tcatgcggct tgcccgggag ttccggcggg
     1741 cagactggcg gcggatgctg tcggatatgt cggccactga gcttggtgag tggggcgatt
     1801 atttccggat gcagagcttc agtgatgtgt ggatggatgc gcagtttgcc tcgctgaagg
     1861 cattgatcgt gagaatggtg tccggcagca gcgatgctgc ggtggctgat ttcagccttt
     1921 taccggaaga gaacgggata ccggagcgaa cggacgaaga actgatgcat cttggggaag
     1981 gtatttccgg aggtgtgcgt tatggaccag atagccaacc tggtcattga tttggggatt
     2041 gatgcggcag agtttaaaaa tgaaattccc cgtatcaaaa accttctgaa tggtgcagcc
     2101 agcgatgcag aacggtcttc tgcccgtatg cagcgtttta tggagcgtca gactcaggcg
     2161 gcccggcaga caatgcaggc ggcttcttcg gctgcaacag cagcatcagc ccatgcgcag
     2221 acggtggaga agaatgcacg ggctcatgaa cgcatggccc gtgaggtgga acaaacccgt
     2281 ctgcgcgtgg atgccctgaa tcagaaaatg cgcgaggaac aggcgcaggc cagggcactg
     2341 gcggaggcgc aggataaagc ggctgccgcc ttttatcgcc agattgacag tgtgaaacag
     2401 gccggtgcgg ggcttcagga attacagcgt attcagcagc agatccgaca ggccagaaac
     2461 agtggcgggg ttggtcagca ggattatctg gcgctgattt cggagatcac ggcgaaaacc
     2521 cgtgccctga cgcaggcaga ggaacaggcc acccggcaga aagcggcgtt tatccgccag
     2581 cttaaagagc aggcaacccg ccagaatctg tcgtcttctg agttgcttcg cgccagggcg
     2641 gctcagctgg gggtaagcag tgctgcagaa gtgtatatcc gcaaaatgga gcgggcagga
     2701 aaagccacac attcgctggg gctgaaaagt gcggcagccc ggcgggagct gggggtgtta
     2761 atcagtcaga tggcgcgcgg caattttggt gcgctgaggg gatccgggat aacgctggct
     2821 aaccgtgccg gatggacagg cgcactgatg tcgccgaaag gcatgatgac tggcggcgtt
     2881 attggcggac ttgtcgcggc ggtcctgggt ctgggtaaag cctggcatga cggccggaag
     2941 gagggcgaga aatttaaccg tcagctggcg ctgacgggac attatgccgg tgtcactgtc
     3001 gggcagttgt ggaaactcag ccgggccata tccgggaatg gtatcacgca acatgcggca
     3061 gccggtgcgc tggcacaggt ggtgggcagt ggggcatttc atggaaacga tattggtatg
     3121 gtggcgaaag ctgccgcaca gatggagcga tcggttggcc agtcggtcag cgataccata
     3181 aatcagttta agcggctgaa ggatgatcct gtaaatgccg cgaaggcgct ggacaatgcg
     3241 ctgcattttt tgaatgccac tcagcttgag cagatacgcg tccttgggga gcaggggcgg
     3301 tccagtgatg cggcccggat cgccatgtct gcgctggcag aggaaaccgg taaacgcaca
     3361 tctgatattg ataataatct caatgcgctg ggtagtacgc tgcaaacctt gtctgactgg
     3421 tggaagcagt tctgggatgc ggccatgaat attggtcgtg aagactcgct ggatgcgcag
     3481 attgatgcgt tacaggaaaa aattcagcgc gcgaaaaaat atccgtggac aaacgcctcc
     3541 acacaggtgg agtacgatca gcagcgtctt aacgatcttc aggagaaaaa acgccggaag
     3601 gatttgcagg atgcaaaagc gcaggcagaa cggaattacc aggagcaaca gaaacgccgg
     3661 aatgctgaaa atgccgcgct gaaccggatg aatgaaacgg aagctgcacg acatcagcgg
     3721 gaaattgcgc gtattaatgc catgcagtac gccgaccagg ctgtcaggga tgcggcgata
     3781 cagcgtgaaa atgaacgtta cgaaaaagcc attaagaaaa atacacgggc aacccgtaat
     3841 gatgaggcca cccggttatt gctgcagtac agtcagcagc aggcacaggt ggaagggcag
     3901 attgctgctg ccagacagtc agcaggcatt gccactgaaa ggatgactga agcgcataaa
     3961 cagcttctgg ccctgcagca gcgcatcagc gacctggacg ggaaaaagct gacggcagat
     4021 gaaaagagtg tgctggcccg taagaatgag ctgattcagg cgctgacgct gctggatgtg
     4081 aaacagcagg agctgcagaa acagacagcg cttaacgacc tgagaaaaaa aacggttcag
     4141 ctgaccagcc agctggcaga caaagaacgt gcactgcgtg agcagcacaa tctggatatt
     4201 gccactgcag gtatggggga taagcagcgg cagcgctacc aggcacagtt gcgcatccgg
     4261 caggaatacc ggcaacagtt gcaacagctt gagaatgaca gtcgccagaa aggcacttac
     4321 gggacggagg actaccggag ggctgaggag gtgctgaagg ggagcctgaa gcgacaactg
     4381 aatgaaaaca aacgctactg gcaggaactg gaagtggcgc agggcgactg gaaaaacggt
     4441 gccatgcggg cgtttcagaa ttttacggcg gatgcggata atgcggcggg aacggcagaa
     4501 cagatgttta cagtggcatt cagcagtgcc ggtaatgcac tggcgacatt ctgtaccacc
     4561 ggtaagctga attttaaatc cttcacctct tccctgttgt cagatatggc cagaattatg
     4621 gcacagatgg ccatgatgca ggcggtaaag ggcgtcggtt ctttattcgg cttcacgact
     4681 aatgctgatg gcggtgttta ccagtctgct gatttgagtc gctacagtgg cacggtggtt
     4741 aaccgtccga cgttttttgc ttttgcaaaa ggcgcgggtg tgatggggga agcgggacct
     4801 gaagccattc tgccactgcg tcgcggtgct gatggtaagc tgggggttgt ggcggatact
     4861 ggtggttcag gcatggtgat gtttgccccg cagtacaaca ttgagatcaa taacgacggc
     4921 acgaacgggc agataggtcc ggctgccctg aaggtggttt atgacctcgg gaaaaaagcg
     4981 gccgcggact ttatgcaaca gcaggcccgt gatggtggcc ggttaagtgg agcataccgg
     5041 taatggagac gtttcactgg aaagtgcgcc cggatatgaa tgtggtatca gagccgaaag
     5101 tggtgacagt gaagctgggc gatggttatg aacagcgtcg tgcggcggga ctgaataacc
     5161 agttgtcgac ttacagcgtg acgatacgtg ttcgtaaagg tgaacaccca tctttaaaag
     5221 cctttctgga acggcacggt ggcgtccgcg catttcagtg gacgccacct tatgactgga
     5281 aactgatcag ggtggtttgt cgtaaatggt cggcaagcgt gggggcgtta tgggtgactg
     5341 taacggccga ttttgaacag gtggtgaact gatgcaggat atccggcagg aaacactgaa
     5401 tgaatgcacc cgtgcggagc agtcagcccg ggtggagctc tgggaaatcg atctgacaga
     5461 ggtcggtggt gagcgttatt ttttctgtaa tgagcagaac gaaaaaggtg agccggtcac
     5521 ctggcagggg cggcagtatc aggcataccc cattcagggg tcggggtttg aactgaacgg
     5581 caggggctgt gctgcccgtc cgacactgac ggtctctaac ctgcacggca tggtcaccgg
     5641 gatggcggaa gatctgcaga gtctggtcgg cggaacggtg gtccggcgta aggtttacgc
     5701 ccgttttctg gatgcggtga acttcgtcaa cggaaacagc gacgccgatc cggaacagga
     5761 ggtgatcagc cgctggcgca tcgagcagtg cagcgaactg agcgcggtca gtgcctcttt
     5821 tgtgctggcc acaccaacgg agacggatgg cgcggttttc ccggggcgta tcatgctggc
     5881 gaatacctgt atgtggactt accgttctga tgagtgtggt tacacgggca gggcagtggc
     5941 tgacgagttc gacaaaccaa caacggatat ccggaaggac aaatgcagca agtgtatgcg
     6001 cgggtgtgag ttgcgcaaca ataccggtaa tttcggcggt ttcctttcca tcaataaact
     6061 ttctcagtaa atccatgaca cagacagaat cagcgattct ggcgcacgcc cggcgatgtg
     6121 cgccagcgga gtcgtgtggc ttcgtggtga gaacgccgga gggagacagg tatcttccca
     6181 gcgagaatat ctccggtgag ccggaggaac ggttccggat ggctccggag gactggctgc
     6241 gggcacaaat gcagggtgag attgtggcac tggtccacag tcatcccggt ggtctgccct
     6301 ggctgagtga ggctgaccgg cggctgcagg tgcagagtga tttgccgtgg tggctggtct
     6361 gccggggggc gattcacaag ttccgctgtg tgccgcatct caccgggcgg cgctttgagc
     6421 acggggtgac ggactgttac acgctgttcc gggatgctta tcatctggcg gggattgaga
     6481 tgccggattt tcatcgcggg gatgactggt ggcgtcacgg tcagaatctc tatctggata
     6541 atctggaggc cacagggctg tatcaggtgc cgttgtcatc agcacaaccg ggcgatgtgc
     6601 tgctgtgctg ttttggttca tcggtgccga atcatgccgc catttactgc ggcgatggtg
     6661 agctgctgca ccatattcct gaacaactga gtaaacgaga gaggtatacc gacaaatggc
     6721 agcgacgcac acactccctc tggcgtcacc gggaatggca cgcatctgcc tttacgggga
     6781 tttgcaacga tttggccgcc gcatcgacct tcgtgtgaaa acgggggctg aagccatccg
     6841 cgcactggcc acacagctcc cggcgtttcg tcagaaactg agtgacggct ggtatcaggt
     6901 gcgcattgcc gggcgtgatg caggtgaaac cgaattgtct gcccgtctta atgagccgct
     6961 ggcaaatggt gcagtgatcc acatagtacc gcgtctggtg ggagctaaaa gtggcggtgt
     7021 gtttcaggcg gtgctggggg cagctgttat ggcggttgct atatggatgc cgggggtagg
     7081 aattatggcg agtaatctgc tgttttctct cggtgccagt atgacgcttg gcggtgttgc
     7141 acagatgctg gcaccgaaag ccagaactcc ccgtacacag acaacggata acggcaaaca
     7201 gaacacctgt ttctcctcac tggataacat ggttgcccag ggcaatgttc tgcctgttct
     7261 gtacggtgaa atgcgcgtgg ggtcgcgggt ggtatctcag gagatcagca cggcagacga
     7321 aggggatggt ggtcaggttg tggtgattgg tcgctgatgc aaaatgtttc atgtgaaacc
     7381 gcctgcgggc ggttttgtcg tttatggagc gtgaggaatg ggtaaaggca gcagtaaggg
     7441 gcataccccg cgcgaagcga aggacaacct gaaatccacg cagttgctga gtgtgattga
     7501 tgccatcagc gaagggccgg ttgacggtcc ggtggatgga ttaaaaagcg tgctgctgaa
     7561 cggtacgccg gtcctggata gcgaggggaa gacaaacttt tccggtgtta cggtggtgtt
     7621 ccgcgccggc gagcaggagc agacaccgcc ggaggggttt gaatcttccg gctcagagac
     7681 tgtgctgggt acggaagtga aatacgacac gccgatcacc cggaccatca cgtcggcaaa
     7741 cattgatcgt ctgcgcctga ccttcggtgt gcaggcactg gtggaaacca cctcaaaggg
     7801 ggaccggaat ccgtcggaag tccgcctgct ggttcagata cagcgtaacg gtggctgggt
     7861 gacggaaaaa gacatcacca ttaagggcaa aaccacttcg cagtatctgg cctcggtggt
     7921 ggtgggtaac ctgccgccgc gcccgtttaa tatccggatg cgcaggatga caccggacag
     7981 caccacagac cagctgcaga acaaaacgct ctggtcgtcg tacaccgaaa tcatcgatgt
     8041 gaaacagtgc tacccgaaca cggcgctggt cggcgtgcag gtggattcag agcagttcgg
     8101 taaccagcag gtgagtcgca attatcatct tcgcgggcgc attctgcagg tgccgtcgaa
     8161 ctataacccg cagacgcggc aatacagcgg tatctgggac ggaacgctta agccagcata
     8221 cagcaacaac atggcctggt gtctgtggga catgctcact catccgcgct acggcatggg
     8281 gaaacgtctt ggtgcggcag atgtggacaa atgggcgctg tatgtcatcg gccagtactg
     8341 cgaccagtca gtgccggacg gatttggcgg cacggagccg cgcatcacct gtaacgctta
     8401 cctgaccaca cagcgtaagg cgtgggatgt tctcagtgat ttctgctcgg cgatgcgctg
     8461 tatgccggta tggaacgggc agacgctgac gttcgtgcag gaccgaccgt cggataaagt
     8521 gtggacctat aaccgcagta atgtggtgat gccggatgat ggcgcgccgt tccgctacag
     8581 cttcagcgcc ctgaaggacc gccataatgt cgttgaggtg aactggattg atccggacaa
     8641 cggtcatgag acggcgacag agcttgtgga ggacacgcag gccattgtcc gttacggtcg
     8701 taacgtcacg aagatggatg cctttggctg taccagccgg ggacaggcgc accgcgccgg
     8761 gctgtggctg attaaaacgg aactgctgga aacgcagacc gtggacttca gcgtcggcgc
     8821 agaagggctt cgccatgtac cgggtgatgt cattgaaatc tgcgatgatg attatgccgg
     8881 tatcagcacc ggtggtcgcg tactggcggt gaacagccag acccggacgc tgacgctcga
     8941 ccgtgaaatc acgctgccgt cctccggtac cacgctgata agcctggttg acggaaatgg
     9001 caatccggtc agcgtggagg ttcagtccgt caccgacggc gtgaaggtga aagtgagccg
     9061 ggttcctgac ggcgttgcag aatacagcgt gtgggggctg aagctgccga cgctgcgcca
     9121 gcgcctgttc cgctgcgtga gtatccgtga gaacgacgac ggcacgtatg ccatcaccgc
     9181 cgtgcagcat gtgccggaaa aagaggccat cgtggataac ggggcacact ttgacggtga
     9241 ccggcgcggc acggtgaatg gtgtcacgcc gccagcggtg cagcatctga ccgccgaagt
     9301 caccgcagac agcggggaat atcaggtgct ggcgcgatgg gacacgccga aggtggtgaa
     9361 gggtgtgagc ttcctgcttc gcctgaccgt ggcagcggac gacggcagtg agcggctggt
     9421 cagcacagcc aggacgacgg aaaccacata ccgcttcagg cagctggcgc tggggaatta
     9481 cagtctgaca gtccgggcgg taaatgcccg ggggcagcag ggcgatccgg cgtcggtatc
     9541 gttccggatt gcggcaccgg cagcgcctgt cactattgaa ctgataccgg ggtattttca
     9601 gataacggtg gtcccgaaac tggctgtata tgacccgacg gtgcagtttg agttctggtt
     9661 ttcggaaaag cggattgcgg atatcaggca ggttgaaacc agcgcccgct atcttggcac
     9721 ggcgctgtac tggataaccg ccagtatcaa tatcaggccg ggccatgatt attattttta
     9781 cgttcgcagt gtgaacaccg ttggcaaatc ggcatttgtg gaggccgtcg gtcgggcgag
     9841 cgatgatgcg gaaggttatc tgagttttta taaagggttg atcaataaaa cgcatctcgg
     9901 caaggagttg tggacgcaga ttgataacgg tcagcttgcg ccggacctga ctgaaatcag
     9961 gacgtccatt acgaatgtca gcaatgaaat cacgcaaacc gtcaataaaa aactggaaaa
    10021 tcagagtgct gcaatccagc agatacagaa agttcaggtt gatacaaata ataacctgaa
    10081 cagcatgtgg gctgtgaagc tgcaacagat gaaggacgga cgcctttata ttgcgggtat
    10141 cggtgccggt attgagaata cgccagcagg aatgcagagt caggtgctgc tggcggcaga
    10201 caggattgcg atgattaatc ctgcgaatgg caacacaaag ccgatgtttg ttggtcaggg
    10261 cgatcagata ttcatgaacg acgtgttcct gaaacgcctg acggctccga ccattaccag
    10321 cggcggtaat cctccggcat tttccctgac acctggcgga cggctgacgg cgaaaaatgc
    10381 cgatatcagc ggtaacgtga acgcgaactc cgggacgctc aacaacgtca cgattaataa
    10441 gaactgtcgg gttctgggaa aattgtccgc gaaccagatt gaaggcgatc tcgttaaaac
    10501 agtgggcaaa cctttcccac gggactcccg ggcaccggag aggtggccat cagggaccat
    10561 taccgtcagg gtttatgacg atcagccgtt tgatcggcaa attgttattc ccgcggtggc
    10621 gtttcgcggt gctaaacatg agcggaagaa taacaatatt tattcgtcat gccgcctgat
    10681 agtgaagaaa aacggtgctg aaatttataa ccgaacgacc ctggataata cgctgatata
    10741 tacgggtgtt attgatatgc ctgccggtca cggtcacatg acgctggagt tttctgtatc
    10801 ggcatggctg gtaaatggct ggtatcccac agcaagtatc agcgatttgc tggttgttgt
    10861 gatgaagaaa tccacagcag gtatcagtat cagctgaatt ttataaccca gatacgggca
    10921 ccagaaatgg tgcctttttt attgcagaaa agcgagaggt aattatgcgt aaactttatg
    10981 ctgccatttt gtccgcagcc atctgtctgg ctgtatccgg tgcgcctgca tgggcgtctg
    11041 aacatcagtc cacgctgagc gcggggtatc ttcatgcctc gacgaacgct cccggcagtg
    11101 atgatctgaa cgggattaac gtgaaatacc gttatgagtt tacggacacg ctggggctgg
    11161 tgacgtcatt cagctatgcc aacgctgaag atgagcaaaa aacgcattac agtgataccc
    11221 gctggcatga ggattccgtg cgtaaccgct ggttcagcgt gatggcgggg ctgtctgtgc
    11281 gcgtgaatga atggttcagc gcgtatgcga tggcgggcgt ggcttacagc cgtgtgtcga
    11341 ccttctccgg ggattatctc cgcgtaactg acaacaaggg gaaaacgcac gatgtgctga
    11401 ccgggagtga tgacaaccgc cacagcaaca cgtctctggc gtggggggct ggcgtgcagt
    11461 ttaacccgac cgaatccgtg gccattgacc ttgcttatga aggttccggc agtggcgact
    11521 ggcgcactga cggtttcatc gtgggtgtcg gttataagtt ctgattagcc aggtaacaca
    11581 gtgttatgac agcccgctgg ttcaggcggg ctttttgtgg ggtgaatatg gcagtaaaga
    11641 tttcaggtgt actgaaagat ggggcgggta aacctgtcgt aaattgtgcg attgaactgc
    11701 gggccagaag aaccagtccg accgttgtgg cacacgttgt tgccacttgc gtgacggaca
    11761 ataacggtgc ttatgtgatt gaggctgagc cggggtatta cgaggttgcg cttcactgta
    11821 acggctggca gccaacccgt gtcggggata ttgatgtggc accgactgat gcaccgggga
    11881 cactgaacgc gtttctgaat gcaccaaagg atggtgattt acgtccggag gtgatgaagc
    11941 gctttgagga aatggtggcg caggcgcagc agagtgccgg ggctgcagcc ggaaacgcac
    12001 agcagacggc gcaggatgtg gcggcagccg caacggcccg tgatgatgca caacgttttg
    12061 cggagaaagc ccgacaggat gcaaccgtca cagctgagga cagaaaggcc actgcggaag
    12121 atgtgacaag cacaggagca aatgcagccg cagccggaca gagcgcacag gatgccgcag
    12181 gttatgcccg cgcagcagaa caggccaaaa atgacattga tgctgcgctg accggcactc
    12241 tgaaaatggc taaccatctg tc
//
LOCUS       DAOFMX010000108        12219 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.108, whole genome shotgun sequence.
ACCESSION   DAOFMX010000108 DAOFMX010000000
VERSION     DAOFMX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.108"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..492)
                     /locus_tag="QBA20_003580"
     CDS             complement(<1..492)
                     /locus_tag="QBA20_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6293982.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="QBA20_003581"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="QBA20_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDQ6293983.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="QBA20_003582"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="QBA20_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDQ6293984.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="QBA20_003583"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="QBA20_003583"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDQ6293985.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="QBA20_003584"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="QBA20_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDQ6293986.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGLVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="QBA20_003585"
     CDS             3646..4155
                     /locus_tag="QBA20_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDQ6293987.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="QBA20_003586"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="QBA20_003586"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDQ6293988.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="QBA20_003587"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="QBA20_003587"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDQ6293989.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="QBA20_003588"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="QBA20_003588"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDQ6293990.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="QBA20_003589"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="QBA20_003589"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDQ6293991.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="QBA20_003590"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="QBA20_003590"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDQ6293992.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="QBA20_003591"
     CDS             complement(8797..9690)
                     /locus_tag="QBA20_003591"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDQ6293993.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="QBA20_003592"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="QBA20_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="QBA20_003593"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="QBA20_003593"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDQ6293994.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11580..>12219
                     /gene="allE"
                     /locus_tag="QBA20_003594"
     CDS             11580..>12219
                     /gene="allE"
                     /locus_tag="QBA20_003594"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDQ6293995.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3156 c         3118 g         2979 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatgcaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcaa
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggcctgg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atcgggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgccg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga taaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg cagaaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttata
//
LOCUS       DAOFMX010000109        12184 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.109, whole genome shotgun sequence.
ACCESSION   DAOFMX010000109 DAOFMX010000000
VERSION     DAOFMX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.109"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(1..396)
                     /locus_tag="QBA20_003595"
                     /pseudo
     CDS             complement(1..396)
                     /locus_tag="QBA20_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            520..1260
                     /locus_tag="QBA20_003596"
     CDS             520..1260
                     /locus_tag="QBA20_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HDQ6293996.1"
                     /translation="MLVNRNIVALFALPFMASATASELSIGADAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(1381..3360)
                     /locus_tag="QBA20_003597"
     CDS             complement(1381..3360)
                     /locus_tag="QBA20_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HDQ6293997.1"
                     /translation="MNISEVDLRKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAEYHELYCAGHLIEAGVAFFQATGRRRLLE
                     VVCRLADHIDSVFGPNEDKLQGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFTSDYDLPNDTVYAE
                     SYASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHRHPEVG"
     gene            complement(3371..4771)
                     /locus_tag="QBA20_003598"
     CDS             complement(3371..4771)
                     /locus_tag="QBA20_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6293998.1"
                     /translation="MTSTPITTADIANGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGHGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            4997..5812
                     /locus_tag="QBA20_003599"
     CDS             4997..5812
                     /locus_tag="QBA20_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6293999.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFQKHTGLTPAAWKRRYSKEHINS"
     gene            complement(5844..6539)
                     /gene="araD"
                     /locus_tag="QBA20_003600"
     CDS             complement(5844..6539)
                     /gene="araD"
                     /locus_tag="QBA20_003600"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDQ6294000.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETYEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6533..7393)
                     /locus_tag="QBA20_003601"
     CDS             complement(6533..7393)
                     /locus_tag="QBA20_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDQ6294001.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWTVEQAAAAQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKALHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(7386..8048)
                     /gene="ulaD"
                     /locus_tag="QBA20_003602"
     CDS             complement(7386..8048)
                     /gene="ulaD"
                     /locus_tag="QBA20_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDQ6294002.1"
                     /translation="MSRPLLQLAPDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     SAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHTMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(8045..9541)
                     /gene="lyxK"
                     /locus_tag="QBA20_003603"
     CDS             complement(8045..9541)
                     /gene="lyxK"
                     /locus_tag="QBA20_003603"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDQ6294003.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVFTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKVG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTLAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            complement(9545..10531)
                     /gene="yiaO"
                     /locus_tag="QBA20_003604"
     CDS             complement(9545..10531)
                     /gene="yiaO"
                     /locus_tag="QBA20_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDQ6294004.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEAITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            complement(10544..11821)
                     /gene="yiaN"
                     /locus_tag="QBA20_003605"
     CDS             complement(10544..11821)
                     /gene="yiaN"
                     /locus_tag="QBA20_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDQ6294005.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(11824..>12184)
                     /locus_tag="QBA20_003606"
     CDS             complement(11824..>12184)
                     /locus_tag="QBA20_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HDQ6294006.1"
                     /translation="LSEHKTLSVSWSHYPIVAFMDNAHVQVTFLVEKLSPAWQRRVAL
                     VTHSLILFICGALAWGATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHL
                     YQLITRSNSLTSPPQGA"
BASE COUNT         2907 a         3211 c         3258 g         2808 t
ORIGIN      
        1 gattgcaggt ttttagcgat aaggtgatat caccgatatc tttaatttta tcgattacat
       61 gtttggataa ataggtaatt atgccggttg gggcgtagat ggtgacccga ccacgtttct
      121 catgcttata atctgcaata aaattattaa gttgctcgtt tctgtccagc atgtcgttga
      181 tgtacggtaa cagcgcggtg ccaaaaggtg tgagcatcag ctgccgggtg gttcggtcaa
      241 agacttttaa acccactttt gattcaaaat cagcaagata tttgctgacg ttggcctgtg
      301 cgatgccaag tacagtccgg catggctgat attttcattg gcagcgatta ccgagataat
      361 ttttaactcc cggtacttaa gttgtaattt tgtcatcgca acagcccaat tatatataat
      421 tttatatatt actatataaa tatgaatctt gcaccggaga aattgtaagc attattatgc
      481 cgactctttt ttatcttatt ttaaaatgga ataccggaca tgttagttaa tcgcaatatt
      541 gtggcgttat ttgcgttgcc ttttatggca agcgcaactg cttctgaatt atccattggt
      601 gctgatgcag cttataatga atcgccttat cgcggttata atgaaaatac aaaggcaatt
      661 ccgctgatta gttatgaagg tgattctttt tatgttcgtc agaccacatt aggttttatt
      721 ctgtcgcaaa gtgaaaaaaa tgaacttagc ctgaccgcat cctggatgcc gctggaattt
      781 gaccctgccg ataatgacga ttatgccatg caacagcttg ataagcgtga tagtacggct
      841 atggcggggg ttgcctggta tcaccacgag cgttggggaa ccgtgaaagc ctctgcggct
      901 gcggacgttc tggataacag caacggctgg gtgggggagc tatcggtatt ccacaaaatg
      961 cagataggtc gcctgtcgct gacacctgcg ctgggcgttc tctattatga cgagaatttc
     1021 agtgactatt actatggcat ttcagagagt gagtcccgtc gtagcggtct ggcaagttat
     1081 tccgcgcagg atgcatgggt gccctatgtc agcctgacgg caaaataccc gataggagag
     1141 cacgtcgtat tgatggcgag cgccggatac agcgagctgc cggaagagat taccgacagc
     1201 ccgatgattg atcgtaatga gagttttacc tttgtcaccg gcgtgagctg gcgtttttaa
     1261 ttcaccggtg gatgtcggtg cggctggcct acgctctcgc accgactcgt atgcctgata
     1321 agacgtgtta agcgtcgcat caggcaatgt gctccctacg ttggatgcgg cgcgagcgcc
     1381 ttatccaacc tccggatggc gatgcttttc ctcattcacc cagatccgca tttcgccttc
     1441 gccccggtta gcccagctaa accacgggat aaaggtcaga gtttgcggct ggcgtttggc
     1501 tggcgcgctg tcgtaatgcc acagcggttg ctgctctgga ttgctctgtt cataccggta
     1561 acccggtgcc tggattaaga tcttatggct gaacaatccc ttgccttcaa atgtcgtaaa
     1621 tggcgcatcg gtgggcagcc acagattatg cagtgactcg ccgttgtcgg cctgttctag
     1681 gcaatacacc agcggaccgc gctgaatcgc cacttttccg gcgacgtgac gcaccagcgg
     1741 gttaccgtaa acgcggcgta ccggcatcgg caaagtcaga ttcagcgtat cgccctcctg
     1801 ccattcgcgg gtaatgtgca aatacccttt acgaatatcc tgctcgacct cttccccatt
     1861 caatatgatc tgcggctgtg tgcaccagtc cggcagacgt aaagccagcg tatgacgtac
     1921 cggctggggc gattcaaccg caatcgtcac ctgctcctgc cacggatagt tcccgctaac
     1981 ccgcaggcgc agcgtgccat tttctaccgg cacttccatg ctgtttcctg cgtatatgtt
     2041 gatatacaac gcatcttcac gcggcgtgta gagataatga ccaatcgagg tcagcacgcg
     2101 ggcgatattt ggcggacaac aagcgcagcc aaaccaacgc tggcggatcg gtttaacgtg
     2161 atcgtagata tggttgaatt tcagcgattt tggatgtact tccagcggat tcacatagaa
     2221 gaaatgtttg ccatccagcg ccatgccgcc gagcacggtg ttgtacagcg cgcgctccat
     2281 cacatcggca tattgactgt cgccttccat ttccagcatt cgccgggcga acatcatcag
     2341 gccgatggaa gcataacttt cggcgtaaac cgtgtcattc ggcagatcgt aatcgctagt
     2401 gaacgcttcg ccgctgcttt gcgagccaat gccgccggta atatataact gacgctgggc
     2461 catattgttc cacagcctca ggcagtcctg acgcttgctg tcatcgtgac ttaaacgcgc
     2521 gagatgcgcg acgccggtca tcaggtagac aaaacgtacc gcgtgaccga tggcggtttg
     2581 ctgttgcgca aggggcaaat gtgcctggct gtaggctttg tctttcacca tccacgccgg
     2641 gccgtaggtg tgccagtgcg aggtctgccc gcgcttttca tattcctggt cgtaatagtg
     2701 cggttgcgca ccacgctgtt cgacaaaata gttcgtcagc gccaggtagc gcggctcttc
     2761 ggtgacttcg tagagacgca tcagcgccag ttcaatttcc ggatgaccag gataaccctg
     2821 cagcttgtct tcatttggac caaatacgct gtcgatatga tcggccagac ggcaaaccac
     2881 ttccagcaag cgccgcctac ccgtggcctg gaagaaggcg actccggctt caatcagatg
     2941 accggcgcag taaagttcat gatactccgc cagattgctc cagcgttctt cgggtgcttt
     3001 taccgtaaag taagtattga gatagccgtc ttcacattgg gcggaggcga tcagttcgat
     3061 tacctcgtcg gcggtttttt ccagttcggc gtccggcttc tggcacagcg accaggctac
     3121 cgcttccagc catttggcga cgtcgctgtc ctgaaacacc atcccgtaaa attcaccctc
     3181 ctgaagtccg gcagcaatgc gaaagttttc aatcgcatgg ctgggttccg cttctgggat
     3241 acgatcgttc aaggcatccc actgataagg aatcaccacg tcgcggacca gttgttggta
     3301 ctgaccgagg aacggatcgc tgaccgtcag tttacgcaga tcgacttccg aaatgttcat
     3361 cttgtgctcc ttaaggctgt acatgacgct gacgcaggtc ggtagctatg cgtgacatca
     3421 taggattatt gagtttgcaa aagcggatcg cccctgccag cagcaggtgg aatagcgcag
     3481 gcagtaatgt ttccatcgcc gcgatcccgc caagcgaact ggcggtctgg ttttccacgc
     3541 caggctggta ggcgacaaaa ataaacagca ggctgatgat cccggcgctg gaggcccagg
     3601 ccagcttgat gaaaaacaga ttgaaggcga agttcatgcc ggaagaacgt acgcgggttt
     3661 tccactcgcc gtagtcatcg gcaaaggcca tcaatgagaa gtgcaatggc aatgtgaagc
     3721 caagaatcac gccattaccg aggatcaccg ccagccacag agtttgataa gccgggccgg
     3781 agggcaggaa ccacatcaac accgccagtg cagcgaggag caggttggtg tagtaataaa
     3841 ttttgacggt atcgaaacgc cgcgttaacg ggctgacaat caccgagccg ataatggagg
     3901 cgaaggtgac catggtgaag aacagcgacg tgtagcccgt actgccttgt aagacgtagg
     3961 taatgaagta catataccca ccgccgcgaa tgttaaagac gttaatcagc aggaaagaca
     4021 tgaccagcat cagcagcagt tggtcgttgt tccgcagccc ggcaagatgt tcgcgcaggg
     4081 taaatttccc cattgtggag agcggcaccc gttcacgaac ccagaagaaa cagcacagaa
     4141 acatcaccac ggcgatggcg cacagcacgc cgacgcccaa ttgataaccg cgcgcggcat
     4201 taccatgacc gaagagatct accatccacg gtaagcctac cgaaaccaga aatcccgcta
     4261 cgccacagag aacgaatcgc caggactggc aggagatcac ttcattgtgg cgggtggtca
     4321 tggtgttgat cagcgcgcag tacggtacgt tgatggcggt ataaccgact gaaaggagca
     4381 ggtaagtacc gaacgcccag gcgattttta ctcccatgct ggcatctggc acggtgaacg
     4441 tcaggatgcc gataatgccg atgggaatgg caatccatag ctgccacgga cgaaagcgcc
     4501 cccaacgact ttgcgtccgg tcggcaatga cgcccatcac cgggtcggag atggcatcaa
     4561 acacgcgcag tgcaataaac agcgtgccaa ccagcgccgg agttaatcca aaaacgtcgg
     4621 tgtagaaaaa ggtgagaaaa ttcatgatca ggcaagttat tacggtgccg cctgcgtcac
     4681 ccaggccgta gcctattttt tcccgtacag ataacgcatc gtcaatttta ccgttagcga
     4741 tatcagcggt agtaatcggt gtagaggtca tagatattcc tcgtgaatag ggagcaattt
     4801 ttagttttat tgaaatgacc tgattaattc tgccgtgaac gggaatatca acaaggcgag
     4861 aaaagagtaa aaaaaggtga cgattccgac ctgaatttaa aagtgtgaaa tggatcaggt
     4921 gaaattaatg tttttgatta ataatcaaaa ggtaatgtat ttaatcatta atgaataagc
     4981 gggaaattac atatcaatgc tcgaattatc catgacgtta ccgattaaag tgcaaaatgg
     5041 tggattattt atttcccgcg gcgtagggag gcatcctgca aggcggctgg agtcatggga
     5101 aattatcttt gtcgaaaaag gtcgattaac gattcaggaa gaagaacgga tatttctggt
     5161 cgatgcggga gagagtttat tactctggcc gaatcgacaa catgttgggg tggaggattt
     5221 tcccgccgat ctcaaatttt actggctgca ttttgaggtt aaagcgccgg atagcgaccc
     5281 gcgctggctc actcatctta gcgtgccgca acaaacgaag gttgccgatc cgcaggcgct
     5341 ggtggccctg tttcgccagt tcttaagcga acaggaaaag catcaacgca ctccggcgct
     5401 ggagtttatt gtgctgctga ttttgcagca gctcacccgt gacgcgcgtc ttgatgaaca
     5461 ggccgatggc gcaggcacca cccttgcctg gaaagcgcag aaactgatcc gcacgcaata
     5521 tcatcgcccg ctttctacgg cgcttctggc ccgcgagctg tactgcaatg ccgattatct
     5581 tgggcgcgtc taccggcggg tgtttcatct gacgttaaca gaagcaatcc atcgccagcg
     5641 agtgcgcgaa gccgaaaagc tgttaattag cgaagcgcga tcgctcactg aagtggctgc
     5701 gttgtgcggg tttaatgacg ttggctattt ccggcagatt tttcagaaac atactggttt
     5761 aacgccagca gcgtggaagc ggcgttacag caaggaacat atcaattcgt agtgccgggg
     5821 cgatgaagcc cctgcgtgag ggattactgc ccgtaatagg cattggcccc atgcttacgc
     5881 aggtagtgct tatccagcag ttcgttttgc atcgcaggga gctgcggcgc aagctggcgc
     5941 gagaataggc ccatataggc gcattcttcg agtactacgg cgttatgcac ggcatcggcg
     6001 gcgtttttac cccatgcgaa cgggccgtga gaatgcacca gcaccgccgg gatttgtgcc
     6061 ggactcctgc cacgttcttc gtaggtttca atgatcactt cgccggtctg atattcatat
     6121 tcgccgttaa tctcctctgc ggtcatctgt cgcgtgcagg ggatggcacc atagaaataa
     6181 tcagcgtgcg tggtgcccca ggcggggaga tccagcccgg cctgcgacca gatggtggcg
     6241 tggcgcgagt gggtatgcac aatgccaccg atttcggcgt agcgacggta aagcgccaga
     6301 tgcgttggcg tgtcggacga gggctttttg ctaccttcca ccaccttgcc gctggctatc
     6361 tcaaccacca ccatatcgtc ggcggtcatc acgtcatact cgacgccgga aggtttgatt
     6421 accatccatt gccgcgtttc gtctaccgcg ctgacattgc cccaggtgaa cgtcaccaga
     6481 tggtgagcgg gaagcgccag attcgccgcc agcacgtcgg ctttcagttg ctctaacata
     6541 taaatccagc ctcctgcata cgcgcttcaa tccaacgccg cgcctgaata atctccagca
     6601 ccggctcttt ggctttttcg gtccacatct caatcaggaa agaacctcga tagttcagtt
     6661 catgcagcgc tttgaagatg ccaacgaaat cgacgcagcc ttcgccaaac ggcacgtcgc
     6721 ggaactgtcc gggactttgc tcggtaactg gctgggtgtc tttcaggtgg atcgcggcga
     6781 tacggtcaat gcccagtttc agttcggcgg gaacatcatt gccccaggcg ctgaggttgc
     6841 cgacgtccgg gtagacggtg aaccacggcg aggcgagcat ctcgtcccac tttttccatt
     6901 tactgatgga gttcataaac gcggtgtcca taatctccac cgccagcatc acctgcgcgg
     6961 ctgctgcctg ttcaaccgtc cacgccagcc cttcagcaaa acgttgccgg gtgccttcat
     7021 cgtggtcttc gtaatagacg tcgtagcccg ccagttggat ggtgcgtatg ccgagatcgc
     7081 gtgccagacg aatcgctttg ctcatgatct cccgtgctcg ctggcgtacc gcgtcgtcac
     7141 ggctaccaaa aggaaagcga cgatgtgcgg acaggcacat cgacgggatc ccaacacccg
     7201 tttcgatcat cgcggcaacc agcgaagtcc tttgtgcggt gctccagtca agacgtgaaa
     7261 gccgttcgtc ggtttcatcc accgacattt cgacaaaatc aaaaccgcag ctttttgcca
     7321 gcaccagccg ctccggccag gagagatctt tcgccagcgc cttttcataa atccctaacg
     7381 gatgattacg cacgcttgcc tccccagata gcgtcgattt gcgcatggaa atcgccagcc
     7441 acctgcgccg gatttgctga gcctgccagt gctcgcccgg caataaacgc tttcacgcga
     7501 atatctttaa acagcggcag gtcagcaggg gtaatcccgc cggtaatgga aagctcaagg
     7561 ccgatatcgg aaagcgcctt catgcgtgcc agatcggctt cgccccactg ttgcccgctg
     7621 gcctgtgcat cacgaccgcg atgataaatc gcctgccgca cgccaatacg atgccagtcg
     7681 cgggcgtcgt ccagcgtcca gttaccgaac aactcgatct gaatttcacc accgcagcgt
     7741 tgcgccatcg tgtggccttt ttccaccgtc gcgagcggcg cggcgcagat gatggtcatc
     7801 cagttagcgc ctgcgccaaa cgcctgttgc gcgagcgttt caccagcgtc ggcgaccttc
     7861 cagtcggcaa cgatgatttt gtccgggcat tgttcgcgca aggctttcac cgcgctaagc
     7921 ccttcgttta aacagagaat ggtgcccgct tcgacgatat cgacgctgtc ttttaacagc
     7981 gtcacgtcgc gctgcgcggc ttcaagtgat gagtggtcgg gtgccagttg cagaagtggt
     8041 cggctcataa tgtgtgctcc ttgatgcggg cgtgaaagcc ctgaagtgcg gcaatgagat
     8101 gctgatagcg ttggtatttt tgttggtaaa gctgatgcgc ggtcatatcc ggcagcaggg
     8161 tgcgtaccgg gtgctgtaag tcacgttgag cttcgctgaa atcgcgataa actccggtgc
     8221 cgacgcgggc ggcaagtgcc gcgccaaaac agccggtttc ttccacctgc ggcagctcga
     8281 tacgcagacc gctgacgtcc gccagcattt gcatccagac atcggagtgc gccgggccgc
     8341 cagtgacgcg cagggtgtgc acatcagtaa aacgttcgcg catccggttg aggtgggtca
     8401 tatggctaaa caccacccct tcatagatgg cctgcaacag gtgcgcgagg gtgtgaatgg
     8461 cctgcatccc gtagaaaccg ctggtcatct cgagtccggc gttgctgccg tacaggaacg
     8521 gcaggaaaaa gagatcgcca ccgaccttcg gcaagctggc aacggcctga ttgatctcat
     8581 caaacgagat ttctccccac tgcgcggtaa accattcgag gttgccggaa gaggtagggc
     8641 tggcttcgtg aacgataaat tgaccatcgt taacgtagcg accatagaca tacggatgcg
     8701 cttcaccgtc acgtaaaccg cgggttatgc cgctggtcac cgcccaggtg cccatcactg
     8761 cgttgagggt gaattcgtct tcgatcccgg cgcagagtgc ggtgaaaacc acatcaaaca
     8821 ggccgccgac tacgggcgta cccgctttca gaccggtcag tgcggctgtc tgagcggtga
     8881 tttccccgca gatttcggca gaaccgacaa caggcggcag ggcgtgattg atttcagcga
     8941 tccccagcca gtcggtgagg cacgggtcat attccccaag actcatgttg tagaggttgg
     9001 actcggaaat attgctctct tcacagcctt tgacgccggt taaacaccag cgcaggtagt
     9061 cgtgcgtcat catcacgcag ccaatttgcg cgtagcgttc tggttcgtgc tctttaagcc
     9121 aacgtagcag cgacaccgga tgcccggtcc acaaggtttg tcgggtcagc gggtagagtt
     9181 tttccgggat gccatcttct tgccagcgac gaacgatttc catcgcccgg cgatccgagg
     9241 acaaaatggc gttaccgagc ggcttgttgt ttttatccag caaaaacaag ccttttcctt
     9301 gtgcggagat accgataccg acaatttgtt ccccgctaac gccggaatgg gtaagcagtt
     9361 cgcgaatgac agccatgcag cattgccaca gttctgccat atcgcgctct gcccaacctg
     9421 gctgcgggct taatgcgcac agcggcaggc gctgcacgcc tgcctcccgg ccttcgcggt
     9481 catacagccc ggctttcagc cagctaccgc cacaatctaa ccccagccag tattgcgtca
     9541 tggtttattg cacctcatcc acggctttca gtagatcagc accctgcgga acatctttta
     9601 caaacatgtc gcggacctga gtacccagtg cgtcgctaaa ggctttgcga tcgagatcgg
     9661 tgatggcttc cacgcccgct tctttcatgc cgtcgatgat tttttgctga tcttcggcaa
     9721 ccagtttgcg ctggtagtta cccgcttcct gcgcagatga aattagcgct tgctggaact
     9781 ccgggcttaa gccatcaaac ttcgctttgt tgatcaccac cagaagcggg gaataggcgt
     9841 ggtgcgtcag agaaaggtac ttctgcactt cgaaaaattt tgctgaccag acgacgttga
     9901 tcgggtgttc ctgagcgtcg atagtgcggg tttccagccc ggtatagact tcggcaaacg
     9961 gcatcgggat cgggttagcg ccaaagactt tgaatgcggc tatattcatc gggctattgt
    10021 tggtgcgaat tttcagccct ttcaggtcgg cgggggtttt aaccggtgcg cgcgagttgg
    10081 tgacatcgcg ccagccgttt tcccagtagg ccagtacttt tagtccttta ccttcaagtg
    10141 aggctttcag atcatcaccg actttgccgt cgagcgtttt atgcgcgtga gcggtatcgc
    10201 ggaacaggaa agggacatca agcaagttca tcactggtga taacccggca aagttattcg
    10261 agccggacat ttccatatcg atggtgccgc cacgtacgcc gctgatcatc gcctgcgcgt
    10321 taccgagggt gctatccgga aacaatttta atttcagctc gcctttggtt ctctcctgca
    10381 ataaatcatt gaattttttc gccgcaatat gttgcgagtc ggtttgtgag gtttcataac
    10441 cgaaacgtaa agattgcgcc gccagagaag atgtgctgaa tgcagccagg ccagcaatga
    10501 ataatgcgta ggttacagag cgtaatttca taatattttc cttttaatta atccatttca
    10561 aagggaggat gatgagatcg ggaataaaaa caaatacgac taataatgaa tataaaacca
    10621 ggacgtaagg gaaaacgcct ctgactgcat catcgaattt gagttttgcc acccctgaaa
    10681 taacgttaag tacattaccg ataggcggtg taattaagcc gattgagcag ttaatgataa
    10741 acatgacacc gaaataaatc ggatcgattc ctgcctcttt aactaaaggc attaataccg
    10801 gggtaagaat taataccgtc ggcgttaaat ccatgaccat gccgacaatc aggatagcca
    10861 ccataatgac gataaacagc agacgcggtg aatcgaccaa cggttgcagt aaatcagaaa
    10921 ccatcatcgg cagttcagca atggtaatca gccaggcgga aacttgtgcc gaggccacca
    10981 gaaacataac cactgaggtg gttttcgccg cgccaatgag tacatgccaa agtgtggcga
    11041 aggtcatttc acggtaaata actgtggcga caaacagcgc atagaaagca gcaacggccc
    11101 cggcttcggt aggggtaaac agaccggagc gaaacccgcc aataatgatg actggaagaa
    11161 acagcgccca gataccggag acaaaagagt gccagatttc ctgcatcgtt gctttttgct
    11221 ggcggggtaa attaaggcga ctggcctgcc gccaccaggt aagcatcagc gttgcgccca
    11281 tcattatgcc aggggcaatg cccgccataa acagcttact gatggataat ccgctggaaa
    11341 caccgaagat aataaacgga atggaagggg gaataattgg cgcgataatg ccgccagagg
    11401 caatcagccc cgccgcccgg ttgaccgggt agttggcgct gcgcatcatc ggcaccagta
    11461 gagcggcaac ggcggcggta tccgcaacgg cagaaccaga aaggctcgcc ataatcatag
    11521 ctgccagcac gccgacgtag cccagcccgc ctggtttatg ccccaccagt ttcatcggca
    11581 ggtcaacaat gcgttttaac aagccgcccg cattcatgat ttcacccgcc agcacaaaga
    11641 acggaatcgc caggagggag aagctatcgg ctccgttcac cagcgtttgt gccatgatct
    11701 ggacatcaaa catgtccagc cagaacatta acgccgcccc gcacaacaac agtgcccagg
    11761 caataggcaa accgatagca ataccaccca acagacagcc cagaaaaatc agcacagcca
    11821 tgattaagct ccttgcggtg gcgacgttag agaattgcta cgcgtgatga gttgatataa
    11881 atgacgcagt tcaaagaatg cgataacgag gctggtgggc agacaagcgg catacatcag
    11941 gccgatgggc aaaccgagga tcggtgaata atcgctccag tcctgaattg tttttagcgt
    12001 tgcgccccag gccagtgcgc cacaaataaa taagattaag gaatgggtaa ccagagcgac
    12061 tcgtcgttgc catgcggggg agagtttctc caccagaaag gtgacctgaa cgtgggcgtt
    12121 atccataaaa gccacaatcg ggtaatgact ccaacttact gatagtgttt tatgttcaga
    12181 taat
//
LOCUS       DAOFMX010000110        12153 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.110, whole genome shotgun sequence.
ACCESSION   DAOFMX010000110 DAOFMX010000000
VERSION     DAOFMX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.110"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            126..995
                     /locus_tag="QBA20_003607"
     CDS             126..995
                     /locus_tag="QBA20_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDQ6294007.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="QBA20_003608"
     CDS             complement(1198..1551)
                     /locus_tag="QBA20_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDQ6294008.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="QBA20_003609"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="QBA20_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDQ6294009.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="QBA20_003610"
     CDS             complement(3379..3672)
                     /locus_tag="QBA20_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDQ6294010.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="QBA20_003611"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="QBA20_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDQ6294011.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="QBA20_003612"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="QBA20_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDQ6294012.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="QBA20_003613"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="QBA20_003613"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDQ6294013.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="QBA20_003614"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="QBA20_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDQ6294014.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="QBA20_003615"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="QBA20_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDQ6294015.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="QBA20_003616"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="QBA20_003616"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDQ6294016.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="QBA20_003617"
     CDS             11052..11627
                     /locus_tag="QBA20_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ6294017.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="QBA20_003618"
     tRNA            11734..11809
                     /locus_tag="QBA20_003618"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2930 a         3140 c         2981 g         3102 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tagagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcca tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctgg cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggctgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tcttccccac atcaccagca atttgcgagc
    11941 atatcccaat ttgggaaaat ttgttcctga gctatagcac taactgacgt gaaatgtcgt
    12001 gcggccccgt gatgctgttg aacgtcaaat gacgtcatca ggagcgtaac gtatccataa
    12061 agcacaacac cggtctgaac gccgactgat gagattttct gaatgagaac aaagagaaat
    12121 gtgtcagtcc gcttgctcat gcaaagacta aca
//
LOCUS       DAOFMX010000111        12112 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.111, whole genome shotgun sequence.
ACCESSION   DAOFMX010000111 DAOFMX010000000
VERSION     DAOFMX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.111"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..370
                     /locus_tag="QBA20_003619"
     CDS             <1..370
                     /locus_tag="QBA20_003619"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294018.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="QBA20_003620"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="QBA20_003620"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDQ6294019.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="QBA20_003621"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="QBA20_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDQ6294020.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="QBA20_003622"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="QBA20_003622"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDQ6294021.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="QBA20_003623"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="QBA20_003623"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDQ6294022.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="QBA20_003624"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="QBA20_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDQ6294023.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..6756)
                     /gene="tar"
                     /locus_tag="QBA20_003625"
     CDS             complement(5095..6756)
                     /gene="tar"
                     /locus_tag="QBA20_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDQ6294024.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVVWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="QBA20_003626"
     CDS             complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="QBA20_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDQ6294025.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="QBA20_003627"
                     /pseudo
     CDS             complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="QBA20_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
     gene            complement(9394..10320)
                     /gene="motB"
                     /locus_tag="QBA20_003628"
     CDS             complement(9394..10320)
                     /gene="motB"
                     /locus_tag="QBA20_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDQ6294026.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10317..11204)
                     /gene="motA"
                     /locus_tag="QBA20_003629"
     CDS             complement(10317..11204)
                     /gene="motA"
                     /locus_tag="QBA20_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDQ6294027.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="QBA20_003630"
     CDS             complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="QBA20_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDQ6294028.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11912..>12112)
                     /locus_tag="QBA20_003631"
     CDS             complement(11912..>12112)
                     /locus_tag="QBA20_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDQ6294029.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2781 a         3389 c         2932 g         3010 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgat ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaagaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccagc gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgcctgg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacaccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagttaacgc
     6361 tgtgtaatag tttttatatt tttcatcaaa attacggctg gtagcgacca tttcaggtaa
     6421 cggtgccatg cttttgaatt ttttataatg cgtcgcggcc tgggccaatg ttttcctggc
     6481 gctatcgagc aattcaactt tggcgttact ttgttgattg gaggaatcca tcatcatccg
     6541 caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg ttgacgtcag
     6601 ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc tatggtgaag
     6661 ggaagaaaaa aacaaactgc cggaaataag ctgtaacagt gcgaataccc ccagcaccat
     6721 taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg aaaacaagtt
     6781 gatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg ccgcgaaatt
     6841 gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa atcgggagaa
     6901 ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt tcagcagttt
     6961 ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag tgagatattc
     7021 tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg tcaatgaaag
     7081 cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat tcaggacgat
     7141 aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc gtaagtcaac
     7201 aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg gcgtgttcgc
     7261 aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat caataccgta
     7321 ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc tggccagctt
     7381 tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc ggcggtgttc
     7441 gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat cagtgccacg
     7501 ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata gttactttca
     7561 aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa ggcgtagcgg
     7621 cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt cgcgcccgcg
     7681 acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac ttccagcacc
     7741 cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga ttccataaca
     7801 gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc gtcgaggatg
     7861 gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt cgactggatt
     7921 tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc gacgccgcgc
     7981 ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat cagcattgca
     8041 acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc ttttgccaga
     8101 attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat gttgccgccc
     8161 tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc gagccgtttt
     8221 tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag cgggtcgata
     8281 atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt cagttctacc
     8341 tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact aaagacatat
     8401 tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc gttacgttgt
     8461 aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc gctggaacgc
     8521 tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa ttgatcaacc
     8581 ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt ttcccgctcc
     8641 acgcggccgg ttggcgcttg ttcggctgcc agtttaagca caggtggggt agctattttt
     8701 ggcgaaactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa acagagtacc
     8761 gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga atccgcccct
     8821 ttcaccacgt catttaacgt tgtcagatgc cccagctctt cttccagcag gtcgacttcg
     8881 ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact ttgctcatct
     8941 tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt ttcgcctttc
     9001 gcttctaatg ccagttgacg caaggcctag cagatataat cgtagctggc ggcatccggc
     9061 tcttgcgact gtttataagc gtcgagctgt tcttgcatga tgtccttcgt ttccaaaaac
     9121 agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc gagcaggttt
     9181 tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc ccctcctttg
     9241 atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc ttccggctgc
     9301 aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc aaaaaatgtc
     9361 tgataaaaat cgcttatatc gatgctcacg ctgtcacctc ggttcggctg atggcattgt
     9421 gggaacactg acctgtggtg caacctcagg tttttctagg gcgcttactg gctcattctg
     9481 gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta ccaacaggct
     9541 gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg ttgccgccat
     9601 gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt cgcggcggga
     9661 tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac cgctggcgta
     9721 gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt tcagcacagg
     9781 cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc cggttctaaa
     9841 catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga ccagatcgat
     9901 tttgagatgg agacgtaacg cccgcagttt cggatcggac tctatcaact ggtcgagatc
     9961 accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt cgatgttcgg
    10021 ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg gaattgggct
    10081 ttcactattg gaaatgcgat cgccgcccgt aaccgctgtc gccagtggag tccggaagta
    10141 ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca tcaccagaaa
    10201 aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc catgtgctgc
    10261 cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt gattcttcat
    10321 gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg ctcttccagt
    10381 tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc aacggcgata
    10441 ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg catcattttg
    10501 ctggtttcgg cgcttttctg acgtaaaaca ctcgctagtg gggaaataaa tccgtaagcc
    10561 aataaaatac cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc cagctcggcg
    10621 gcaggacgat cggctgaacc taacgcgtga acgcccccca ttacagccgc gacaatacca
    10681 aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc tgcctcgctt
    10741 tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt catgtgaccg
    10801 ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc cgcgaggatg
    10861 cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg ttccagcgaa
    10921 aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag cagatccata
    10981 tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt cagcgtgcct
    11041 ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc ggcaataatc
    11101 accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa ataaccgccg
    11161 aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc atccttccac
    11221 tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg gaaaaaaaaa
    11281 gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag ttaaacagcc
    11341 tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg ggaaagttta
    11401 cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct gccaacaggc
    11461 tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag ttgcagtaat
    11521 ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag tggtccttct
    11581 tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc atcgacgcca
    11641 ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga agcatgaacg
    11701 ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt cggcggtggg
    11761 cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg tgtttcgctt
    11821 tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg aatatcccgc
    11881 gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc agcgcttctt
    11941 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    12001 ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac
    12061 ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga ag
//
LOCUS       DAOFMX010000112        12084 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.112, whole genome shotgun sequence.
ACCESSION   DAOFMX010000112 DAOFMX010000000
VERSION     DAOFMX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.112"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(26..402)
                     /gene="rnpB"
                     /locus_tag="QBA20_003632"
     ncRNA           complement(26..402)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="QBA20_003632"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(435..1580)
                     /gene="garK"
                     /locus_tag="QBA20_003633"
     CDS             complement(435..1580)
                     /gene="garK"
                     /locus_tag="QBA20_003633"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDQ6294030.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1677..2567)
                     /gene="garR"
                     /locus_tag="QBA20_003634"
     CDS             complement(1677..2567)
                     /gene="garR"
                     /locus_tag="QBA20_003634"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDQ6294031.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2597..3367)
                     /gene="garL"
                     /locus_tag="QBA20_003635"
                     /pseudo
     CDS             complement(2597..3367)
                     /gene="garL"
                     /locus_tag="QBA20_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3383..4717)
                     /gene="garP"
                     /locus_tag="QBA20_003636"
     CDS             complement(3383..4717)
                     /gene="garP"
                     /locus_tag="QBA20_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDQ6294032.1"
                     /translation="MILDTVDAKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5092..6663
                     /gene="garD"
                     /locus_tag="QBA20_003637"
     CDS             5092..6663
                     /gene="garD"
                     /locus_tag="QBA20_003637"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDQ6294033.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6715..7539)
                     /gene="agaR"
                     /locus_tag="QBA20_003638"
     CDS             complement(6715..7539)
                     /gene="agaR"
                     /locus_tag="QBA20_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDQ6294034.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7788..9068
                     /gene="kbaZ"
                     /locus_tag="QBA20_003639"
     CDS             7788..9068
                     /gene="kbaZ"
                     /locus_tag="QBA20_003639"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDQ6294035.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9091..9564
                     /gene="agaV"
                     /locus_tag="QBA20_003640"
     CDS             9091..9564
                     /gene="agaV"
                     /locus_tag="QBA20_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDQ6294036.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9575..10354
                     /locus_tag="QBA20_003641"
     CDS             9575..10354
                     /locus_tag="QBA20_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDQ6294037.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10344..11153
                     /locus_tag="QBA20_003642"
     CDS             10344..11153
                     /locus_tag="QBA20_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDQ6294038.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11240..11674
                     /locus_tag="QBA20_003643"
     CDS             11240..11674
                     /locus_tag="QBA20_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDQ6294039.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDVLGRCHEECPVEEGI"
     gene            11671..>12084
                     /locus_tag="QBA20_003644"
     CDS             11671..>12084
                     /locus_tag="QBA20_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDQ6294040.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2878 a         3254 c         3087 g         2865 t
ORIGIN      
        1 gccgaagcgg gtttttacgt aaaacaggtg aaactgaccg ataagccggg ttctgtcgtg
       61 gacagtcatt catctaggcc agcaatcgct cactggctca agcagcctac ccgggttcag
      121 tacgggccgt accttatgaa cccctatttg gccttgctcc gggtggagtt taccgtgcca
      181 cggactgtta ccagccgcgc ggtgcgctct taccgcaccc tttcaccctt acctgatccc
      241 gcttgcacgg gccatcggcg gtttgctctc tgttgcactg gtcgtgggtt tcccccccag
      301 gcgttacctg gcaccctgcc ctatggagcc cggactttcc tcccctccgc ccgtctcccc
      361 cgaagaggac gacgacgaag cggcgactgt ctggtcagct tcggcgcgca gtatagaggg
      421 tttgcgcgcc cttgtcaccc cgtgttgcgc attccaatcg ccagtgtcgc ggcaatatta
      481 cgtgaagcac ggcagatatt gtcataagcc ccgcggaatg cttcgtccaa cgtacctatg
      541 ctggtcaata cgctgaagac cgcatcaatg ccatgctgat gtacaacgcc aacatcatcg
      601 gtcaggctac ccgcaatgcc aatcaccggt ttatggtact tcttcgccac gtttgcgacg
      661 ccaatcggta ccttgccgtg aatactctgg ctgtcaatac gcccttcacc ggtgatcacc
      721 agcgtacaat cgtgaatatg ttcctccaga ttcagcgccg tagtgacgat ttcaatacca
      781 cttttcagtt ccgcaccaag aaacgccatc agcgccgcac ccataccgcc cgcagctcct
      841 gcgccgggga catcttttac atcaacatgc agcgcttttt taatgacatc ggcatagtga
      901 gagaggttat tgtccagctc aacaatcatc gcttcactgg ctcccttttg tgggccaaag
      961 atgcgcgatg cgccgttatc gcccaccagc ggattggtga catcacaagc gacgcgaatg
     1021 acgcaatctt ttaagcgcgg atcgaggccg gaaatatcaa tatcattcag agtattaaga
     1081 ctaccgccgc caaaaccaat ttcattgccg ttggcgtcgc ataatttcgc ccccagcgcc
     1141 tgtaccatgc ctgcgccgcc atcatttgta gcgctaccgc caatgccgat aataatgttt
     1201 gtcgcgccgc tctccagcgc ctgcaggatt aactcgcctg tgccgcgtga agtggtcacg
     1261 agtggatcgc gtttttccgc aggtaccagc tccagcccac tggccgccgc catttcaata
     1321 aacgcggttt tgccatcgcc ggagatcccc caactggcat tcactttctc gcccagcggc
     1381 cctgtaaccc aggcgtgacg ttcagaaccc tgggtggctg caatcatcgc ttccaccgtt
     1441 ccttcgccac cgtcggcaac cggaatagaa acgtactgtg catcaggaaa aatttcccga
     1501 aatccttttt ctatcgcctg cgcaacctcg ctggcagata aactttcttt ataagagtct
     1561 ggggcgatta cgattttcat acctatgcct gttaccacat gacgccggag ggcgtttctc
     1621 ttattcggcc tggattccag gcccggattg caatacgcca tccgggcacg acgtcattaa
     1681 cgagtaactt cgactttcgc cagtttttcg tagtagcacg ccagggcgct atgatccgcc
     1741 gttcctaaac catctgctcg cagtgcctgc atcatctcca taaccgcagc tgtgagcggc
     1801 agttgcgcgc cgacgccgtg ggaagtatcc agcgcatttg ccagatcctt aatatgcaga
     1861 tcaatacgga agcccggctt gaagttgcgg tccatcacca tcggcgcttt ggcatccagc
     1921 acggtactgc ccgccagtcc accgcgaatt gcctgataca ccaggtccgg gttaacgccc
     1981 gctttagttg ccagcgttaa cgcttctgac atcgcggcaa tattcagcgc cacaatgacc
     2041 tgattcgcca gtttggtgac gttacctgca ccaattgccc cggtatgcac cacggaaccc
     2101 gccattgctt tcatcaaatc atagtatttg tcgaaaatag ccttgtcgcc gcccaccatc
     2161 actgacagcg taccgtcgat ggctttcggt tcaccgccgc tcaccggagc atccagcata
     2221 tcaatgcctt tcgctttcag cgcttcgctg atttcacggc ttgccagcgg tgcgatagaa
     2281 ctcatatcga tcaataccgt acctggcttc gcgccttcaa taatgccatt ctcacccagc
     2341 gccacctctt tcacatgagg ggagtttggc agcatggtta tgatgacgtc gcactgttca
     2401 gcgatcgctt tagccgtaga ggctgtttct gcacctgcag caatcacgtc agcaatagct
     2461 tctgggttac ggtcagcaac caccagcgag taacctgctt tcagaaggtt tttactcatt
     2521 ggtttaccca taatccccag gccaataaaa ccaactttca tagtcatatc aatcatctct
     2581 cttgttgcgg tggtggttat tttttaaagg tatcagccag tttatgagtg gcagagcgga
     2641 agacgccgag atcgctgccg acagccacaa acgtcgcgcc ccattccaga taacgacgcg
     2701 catcggcttc gaccggcgcg aggataccgc tgggtttgcc gtgcgcgctg gcacggttaa
     2761 aaatgtgctg aattgctttt tgtacatccg ggtgtgatgc attgccgaga tggcctaatg
     2821 ccgcggccag atcgctgggg ccgacgaaga tgccgtctac gccttcggta gcggcaatgg
     2881 catcgacgtt atctacgccc tgctgacttt ctatctggac cagaatagtg atgttcttgt
     2941 tcgactgagc gaaataatcc gccacggtgc caaacatatt ggcgcggtga gaaacggaga
     3001 cgccgcgaat gccttccggt gggtaacggg ttgatgccac cgcctgctct gcttcctctt
     3061 ttgtttctac aaaaggaatc aggaagttat agaaaccgat atccagaaga cgcttaataa
     3121 tttccggctc gttggtcggc actcacacta ctggcgcgct ggcgctgcct ttcaaggcca
     3181 ttaactgcgg aataaacgtg gagatatcgt ttggcgcatg ttcgccatcc agcaccagcc
     3241 agtcaaaccc agccaaacca agaacttcag tgctaatcgg gttagagagt gctgaccagc
     3301 aaccaatttg tacctgtttc gcagccagtg cggctttgaa tttattcggg aaaacatcgt
     3361 tattcatcgc ttataccttt gcttatttct gcaattccat acgtttaatg tcgccaacta
     3421 cgaagaggta gcagaccatc gccatcagcg ctgaacatcc cacgaaaacc agtgctgcat
     3481 tgaaggagtg cagttcactt accaggtagc caatcaccag tggagtgaca atggaggcaa
     3541 cattgccaaa gacgttaaag acgccgccac agaggccaac aatctctttc ggcgcggtgt
     3601 cagaaatcac cggccagccc agagcaccaa atcctttgcc aaaaaaagcc agcgccatca
     3661 gcatgaccac cagcgtggtg ttgttggtgt agttacataa gatgatggtg gaagccagca
     3721 acattcccag cacaatcggt agcttacgtg ccagggtcag ggataaaccg cgtttgatca
     3781 gataatccga gaagacacct cccagcacgc cgcccgcaaa accacacagt gccggaatcg
     3841 aggcgaccag acccactttc agaatcgaca tgcctttttc ctgcaccaga taaatcggga
     3901 accaggtgag gaagaaccag gtgatggtgt tgataaaata ttgtccgaaa aatacgccca
     3961 acatcatgcg gttagagagc aattgcttga tgtaatgcag tttgggaccg cttgctgccg
     4021 cgctgcccgg ctttttgtgg tccatatcga ccaccgcgcc attttcagag ataaacttca
     4081 gctcttcctc agacatacgt gggtgatctg tcgggttatg aatcaacttg atccacagcg
     4141 ccgtcagcac aaaacaaatc acccccataa cggtaaagac gtgctcccat ccccaggcga
     4201 aagtcagcca gccaagcaga ggcgaaaaga gcgccagcga gaaatattgt gccgagttaa
     4261 agatggcgga ggcagtacca cgttctttcg tcgggaacca ggcggcgaca atacgggcgt
     4321 tcgccgggaa tgatggagct tccgagaatc ccagcataaa gcgcataaag aacatggaga
     4381 tccctgccca ggccagcggg aacatatcaa caaagccttg caggaaggtg aacagcgacc
     4441 agaaaaagag gctgtaggtg taaacttttt tcgagccaaa cttatcaagc aaccagccgc
     4501 cggggatttg catcagcaag taggcccagc caaaagcgga gaagatgtaa cccatcgaaa
     4561 ccgcacttaa ctgcaactct tttgccactt cggtaccagc aatagacagc gttgcacgat
     4621 cggcgtagtt aacggcggta acaataaaaa taatcagtaa tattaaatag cgggtatgca
     4681 cacctttcct ttttgcgtca acggtgtcca gaatcatttt atttacctcg ggtacttatg
     4741 ctgattttta ttattatggg gaaggtgtta tttataagtt tcatttatgc cgtaacgaca
     4801 atgaactcgg gaattagtat aagcagcgcg agaataataa tcattgtgca aatgctaatt
     4861 taattaatac tttttaaata ttattttgag catatgcaca taaggttgcg cgctaaagca
     4921 cagatttgcg ctttaccttg ccgggcggca ctgcaatccc tgaaatgatt gacattgatc
     4981 acatttctgc gtttaaactc ctgacattct tatttcaccc aatgaaatca tttattttta
     5041 aatgagacca ggtcctcatt ttaataaccc ctggctggag aatattgcac aatggccaac
     5101 atcgaaatca gacaagaaac gccaactgcg ttttatataa aagttcacga cacagataat
     5161 gtggcaatta ttgttaatga taatggcctg aaagcaggaa cgcgttttcc ggatgggctg
     5221 gaattaattg aacatattcc ccaggggcat aaagtcgcat tgctggacat tccggctaat
     5281 ggtgaaatta ttcgttatgg cgaagtgatt ggttacgccg tgcgtgcaat cccacgcgga
     5341 agctggatcg acgaatcaat ggttgtacta ccggaagcgc cgccgttaca cacgctgcca
     5401 ctggcaacca aagtcccgga acccttaccg ccgctggaag gatacacctt tgagggctat
     5461 cgcaatgccg atggcagcgt gggcaccaaa aacctgctcg gcatcaccac cagcgtccac
     5521 tgtgtggcag gcgtggtgga ctatgtagta aaaatcattg aacgcgatct gctaccgaaa
     5581 tacccgaacg tcgatggcgt ggtggggctg aatcatttgt acggttgtgg cgtggcgatt
     5641 aacgcaccgg cagcagttgt acctattcgt accattcaca atatttcgct gaaccctaac
     5701 tttggcggcg aagtaatggt gattggcctg ggttgtgaaa agttgcagcc tgagcgcctg
     5761 ctgactggaa cggatgatgt gcaagctatt ccagtagaaa tcgccagcat tgtcagtttg
     5821 caggatgaaa agcatgtcgg ttttcagtcc atggtcgagg atattttgca ggtcgccgaa
     5881 cgccatctac aaaaactgaa tcaacgtcag cgagaaacct gtccggcttc agaactggtt
     5941 gtcggcatgc attgcggtgg cagcgatgcg ttttctggcg taacggcaaa cccggcggtt
     6001 ggctatgcgt ctgatctact ggtgcgctgc ggcgcaacgg tgatgttttc agaagtcacg
     6061 gaagtgcgtg acgcgatcca tctgctgaca ccacgcgcag tgaacgaaga ggtcggcaaa
     6121 cggctgctgg aggagatgga gtggtacgat aactatctca atatgggaaa aaccgaccgc
     6181 agcgccaacc cttcgccggg caacaagaaa ggcggtctgg caaacgtggt agagaaagcg
     6241 ctcggctcca ttgctaaatc gggcaaaagt gcaattgttg aagtgctgtc gcccggtcaa
     6301 cgcccgacta aacgcggatt aatttacgcc gcgacgccag ccagcgattt tgtctgtggc
     6361 acgcaacagg tggcttcggg tatcaccgtg caagtgttta cgaccggtcg tggtacgccg
     6421 tacggcctga tggcggtacc cgtcattaaa atggcaaccc gcaccgagct ggcgaaccgc
     6481 tggtttgatt taatggatat taatgcgggc accatcgcta ccggcgaaga aactattgaa
     6541 gaggtgggct ggaagttgtt ccactttatt ctcgacgtcg ccagcgggaa gaagaaaacc
     6601 ttctcggatc aatggggact gcataaccag ctggcggtgt ttaacccagc accggtgacc
     6661 tgataattca attcgtatta tgcccgatag cacacgttta tcgggcatat tgagtcacag
     6721 cgaagacgcg tgatccccga ccagaatcac ttcaacccca gcctttcgca gtccttccag
     6781 actatccgca ggaatgcctt catcaacaat gatcatgtcg atacgttgag tatcaatgat
     6841 cttatgtaaa ctggaacgat tgaatttact ggaatcggtg accacgatga tccgttccgc
     6901 aacttcgcac atccgacggt ttaaacgggc ttcatcttca ttatgtgtgc tgacgccgcg
     6961 ctccagatcg atcgcatcta caccaagaaa cagcatatcg aagtggtaat tttgcagcga
     7021 ttgctcagcc tgatcgccgt aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat
     7081 cagcagctca acgccttccg cttccagcaa cgcattagcc acgttcatac cgttggtcat
     7141 cgcaattacg tcagtgtact tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga
     7201 atcgaggatc acccgatgac ctggctgaat caactcaacg gcagctttcg caacgctgcg
     7261 tttcatcgcg gtgttcagtg cgcttttatc ttccactgat ggctcgactg acggcgtcgt
     7321 gctatcgcag atcaacgcgc caccataggc acgcacagcg atcccctgct tttccagaaa
     7381 cgccagatca ttgcggatcg tcacagtaga tacgccatac aatgccgaca gatcgttaac
     7441 ctgcacactc ccttgctgtc gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc
     7501 tgtcactcgc ttctcacctg aagcgtcggt attactcata gtaagtcctt tcgtaaaact
     7561 ttcgtttcat ttcgttttgc ctattaacgc ctttctatta agcaaatgca agcccacctt
     7621 gcccattggc gcaagctact ctcgtttcag tgactttcat tacgtttctt ttgtgaatca
     7681 gatcagaaaa ctattatctt tcgttttatt tttatctcac catgacgcag tatcaattga
     7741 aacaaaacga aagatcaata tcgcagtaat ctgaactgga gaggaaagtg aaacatctga
     7801 cagaaatggt gagacagcac aaagcgggca aaacaaatgc aatttatgcc gtttgttccg
     7861 cacatccgct ggtgctggag gctgcaatcc gctacgccag tgcaaaccaa acgccgttac
     7921 tgattgaagc aacctccaat caggtagacc agttcggcgg ttataccgga atgacgcccg
     7981 ccgattttcg cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg caggatgcgc
     8041 tgattctggg tggtgaccat ctggggccaa accgctggca aaacctgcca gccgctcagg
     8101 caatggccaa tgccgatgat ttgattaaaa gctacgttgc ggcaggattc aaaaaaatcc
     8161 accttgattg cagcatgtcc tgtcaggacg atccaattcc cttaactgat gacatcgtgg
     8221 ctgaacgcgc cgcccgtctg gcgaaagtgg cggaagaaac ctgtcgcgaa cactttggcg
     8281 aagccgatct ggagtatgtc attggtaccg aagtgccggt acctggcggc gcgcatgaaa
     8341 ccttaagcga gctggcggtc accacgccgg atgccgcccg cgccacgctg gaagcccatc
     8401 gtcacgcctt tgaaaagcaa ggtttgaatg ccatctggcc acgcattatt gccctggtgg
     8461 ttcaacccgg cgtcgaattc gatcacacca acgttattga ttatcagccc gccaaagcga
     8521 gcgccttaag ccagatggtc gaaaactacg aaacgctgat tttcgaagcg cactctaccg
     8581 attatcaaac gccgcaatct ctgcgccagc tggtgattga ccactttgcc attctgaaag
     8641 ttggcccggc gctgaccttc gccctgcgtg aagccctttt ttctctggcg gcgattgaag
     8701 aagaactggt accagcgaaa gcctgttccg gtctgcgtca ggtgctggaa aacgtgatgc
     8761 tcgaccgccc ggaatactgg caaagccact accacggtga cggcaacgcg cgtcgtctgg
     8821 cgcgtggtta cagctactca gatcgcgtgc gctattactg gccggacagc cagattgatg
     8881 acgctttcgc tcatctggtt cgtaatctgg cggattcacc aattccgcta ccgctcatca
     8941 gccagtatct gccgctgcag tacgtgaaag ttcgctccgg cgagctgcaa ccgacgccac
     9001 gggaactcat tataaaccat attcaggaca tcctggcgca gtaccacaca gcctgtgaag
     9061 gccaataagc aaaacaaaga ggaacatgct atgccaaata ttgttttaag ccggattgat
     9121 gaacgcttga ttcacggtca ggtcggcgtt cagtgggtcg gatttgcggg ggcaaatctg
     9181 gtgctggtag ccaacgatga ggttgccgaa gatccggtac aacaaaacct gatggaaatg
     9241 gtactggcag aagggatcgc cgtacgtttc tggacgctgc aaaaagttat cgacaacatt
     9301 catcgcgccg ccgatcgaca gaaaatcctg ctggtttgta aaacacccgc cgatttcctg
     9361 acgctggtga aaggtggcgt tccggtgaat cgcattaacg ttggcaatat gcactacgcc
     9421 aatggcaaac aacaaatcgc caaaacggtt tctgtggatg cgggcgatat cgcagcattt
     9481 aacgacctga aagccgctgg ggtggaatgc ttcgttcagg gcgtcccgac agagcctgct
     9541 gtggacctct ttaaattact ttgagggatt catcatggaa atcagcctgt tgcaggcatt
     9601 tgcgttgggc attatcgcct ttatcgctgg cctagatatg tttaacggct taacccatat
     9661 gcaccgcccg gtggtcctcg gcccgttggt cgggctggta cttggcgatc tgcataccgg
     9721 aattttaacc ggcgatacgc tggaactagt gtggatgggg ctggccccgc tggcgggcgc
     9781 acagccgcct aacgtgatta tcggtactat cgtcggcacg gcgtttgcca ttactactgg
     9841 cgtgaaaccc gatgtcgcgg tcggtgtcgc cgtacctttc gctgtcgcag tacagatggg
     9901 gattaccttc ctgttctcgg tgatgtctgg cgtgatgtct cgctgcgacc ggatggcgga
     9961 gaacgccgat actcgcggca ttgaacgcgt gaactatctg gcactgctgg cactcggcac
    10021 tttctatttt ctctgcgctt tcctgcctat ctacttcggc gcggaacatg caaaaaccat
    10081 cattgatgtc ctgccgcaac gattaatcga cggcctcggc gtcgcgggcg gcatcatgcc
    10141 agcaatcggt tttgccgtgc tgctgaaaat catgatgaaa aacgtctaca taccctactt
    10201 catcctgggc tttgttgccg caacctggct caagttaccg gtgctggcta ttgctgccgc
    10261 cgcgctggcg atggcgctga tcgatctgct gcgtaaatct cctgaaccga ctcaacctgc
    10321 ggcacagaaa gaggaatttg aagatggcat ctaatcaaac caccctgccg aacgtcagcg
    10381 aaaatgaaga aacactgctg actggcgtca atgaaaacgt gtatgaagat cggagcattg
    10441 ccgcggagct gacgaaaaaa gatatcaacc gcgtcgcctg gcgttccatg ctgttacaag
    10501 cgtcttttaa ctacgaacgt atgcaggctt ctggctggtt gtacggtctg ctgcctgctc
    10561 tgaaaaagat ccacactaat aaacgcgacc tggcgcgcgc catgaagggg catatgggtt
    10621 tcttcaatac ccatccgttt ctggtgacgt ttgttatcgg cattatcctt gcgatggagc
    10681 gttctaagca ggacgttaac agtattcaga gcaccaaaat tgccgtcggt gcgccgcttg
    10741 gcgggattgg cgatgcgatg ttctggctaa cgctactgcc gatttgtggc gggataggcg
    10801 caagcctcgc gctacaaggc tctattcttg gcgcagtcgt ctttattgtg ctgttcaacg
    10861 tggtacactt ggggctgcgt tttggtctgg cgcattatgc ttaccgcatg ggcgtggcgg
    10921 cgattcccct tattaaagcg aataccaaaa aagtcggcca tgctgcgtca atcgttggaa
    10981 tgacggtaat cggcgcactg gtggcaacct atgttcgttt aagcaccacg ctggaaatca
    11041 cggcgggcga cgcagtagtt aagttacagg ctgatgttat cgacaaactg atgcctgcct
    11101 tcttaccgct ggtctacacc ctgaccatgt tctggctggt acgccgcggc tagagtccgc
    11161 tgcgcctgat tgcggttacc gtggttctag ggatcgtcgg taaattctgc catttccttt
    11221 aaatacaatg aggtattaga tgttaagtat tattttgaca gggcatggcg ggtttgccag
    11281 tggcatggaa aaagcaatga agcaaatcct cggcgatcaa tcgcagttta ttgccatcga
    11341 ttttcccgaa acctcaagcg ccgcgctgct tacatcgcag cttgaagagg ccatcgccca
    11401 actggattgt gaagacggca ttgtttttct gacggatttg ctgggcggca cgccgtttcg
    11461 cgtagcttca acgctggcga tgcaaaaacc gggctgtgaa gtgattaccg gcaccaattt
    11521 gcaactgcta ctggagatgg tgctggagcg cgaagggtta agcggtgaag aattccgtgt
    11581 acaggcgctg gagtgtggac accgagggct gaccagtctg gtggacgtgt taggccgctg
    11641 ccatgaagaa tgtccggtcg aggaaggaat atgacacacg ttctgcgcgc cagaaggctg
    11701 ctgactgaag agggatggtt cgatgaccat cagttgcgtg ttgctgacgg tgtcatcgca
    11761 gcaatcgaac cgattccagt gggcgtgact gaacgcgatg cggaactgct ctgccccgct
    11821 tacatcgaca cccatgtaca cggtggtgcg ggcgttgatg taatggatga cgcgccggat
    11881 gtactcgaca agctggcaat gcacaaggca cgcgaaggtg tcggcagttg gttaccgacc
    11941 accgtaaccg cgccgcttaa taccattcat gcggcgctga aacgtattgc tcaacgttgc
    12001 caacgcggcg gacctggtgc gcaagtgctg gggagttatc tcgaaggacc gtacttcacg
    12061 ccgcagaata aaggcgcgca tccg
//
LOCUS       DAOFMX010000113        11921 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.113, whole genome shotgun sequence.
ACCESSION   DAOFMX010000113 DAOFMX010000000
VERSION     DAOFMX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.113"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..1093
                     /locus_tag="QBA20_003645"
                     /pseudo
     CDS             1..1093
                     /locus_tag="QBA20_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(1217..1987)
                     /gene="glcC"
                     /locus_tag="QBA20_003646"
     CDS             complement(1217..1987)
                     /gene="glcC"
                     /locus_tag="QBA20_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDQ6294041.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2238..3737
                     /gene="glcD"
                     /locus_tag="QBA20_003647"
                     /pseudo
     CDS             2238..3737
                     /gene="glcD"
                     /locus_tag="QBA20_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            3737..4789
                     /gene="glcE"
                     /locus_tag="QBA20_003648"
     CDS             3737..4789
                     /gene="glcE"
                     /locus_tag="QBA20_003648"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDQ6294042.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4800..6019
                     /gene="glcF"
                     /locus_tag="QBA20_003649"
                     /pseudo
     CDS             4800..6019
                     /gene="glcF"
                     /locus_tag="QBA20_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6024..6428
                     /locus_tag="QBA20_003650"
     CDS             6024..6428
                     /locus_tag="QBA20_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HDQ6294043.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            6450..8621
                     /gene="glcB"
                     /locus_tag="QBA20_003651"
     CDS             6450..8621
                     /gene="glcB"
                     /locus_tag="QBA20_003651"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDQ6294044.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRGNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8976..10572
                     /locus_tag="QBA20_003652"
                     /pseudo
     CDS             8976..10572
                     /locus_tag="QBA20_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
BASE COUNT         2887 a         2867 c         3241 g         2926 t
ORIGIN      
        1 atgaccttaa ggcatttatc gcttttccgt catcacttta tcccgacgat ccaaactgga
       61 tacccccttt attcattgaa cgcaacgagc atttgtctgc gaaaaatccg gggacggacc
      121 atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca gatagttggg cgtattaccg
      181 cgcaaataga taccttgcac cgtgagcgtt acggtgaaga taccggtcat ttcggcatga
      241 ttgacgccat tgatgatccg caggattttg ccgcgttgtt tggcgcagcg gaagcgtggt
      301 tgaagtcaca aggtgcaagt aagatcagcg ggcctttcag cctgaatatc aatcaggaaa
      361 gcggattact gattgaaggt tttgacacac caccctgtgc gatgatgcca cacggcaaac
      421 cgtggtatgc cgcgcatatt gaacaactgg gttatcacaa aggcattgat ttactggcgt
      481 ggtggatgca gcgaaccgat ctcactttct ctccggcgct aaaaaaactg atggatcagg
      541 tgcgcaaaaa ggtgaccatt cgctgcatca atcgtcagcg gtttgccgaa gagatgcaga
      601 tcctgcgtga gattttcaac tctggctggc agcacaactg gggatttgtg ccgtttaccg
      661 aacatgaatt cgcgaccatg ggcgaccaac ttaaatatct ggtgccggac gatatgatct
      721 acatcgctga gattgattct gcaccctgcg cgtttattgt ggcttgccta acatcaacga
      781 ggcgattgcc gatctgaacg gatcgctctt tcccttcggc tgggcaaaat tgctgtggcg
      841 tttgaaagtc agcggtgtgc gaactgcgcg agtaccgctg atgggcgtgc gcgacgagta
      901 tcagttcagc cgcatcggcc cggtgattgc gctgttattg attgaagcct tacgcgatcc
      961 gtttgcccgc cggaagatcg atgcgctgga gatgtcatgg atcctcgaaa ccaatacagg
     1021 catgaataac atgctggaac gcattggggc ggagccgtac aagcgttacc gattgtatga
     1081 aaagcaaatt taataccaga tcctgtttag cgcaggggcg gtcattcggg agagggtata
     1141 gatgaacaga tttaacacca gatcctgtta agtgcagggg cggtcccctc gccttgggga
     1201 tagggtaaaa ctctgctcac attcccacct tgttcatctc cagcggcacc gccgagcgaa
     1261 tcaaatggtg gccttccagc tcgatatcgt ggagattctt tttcacggtc cgtacatgat
     1321 cgcgcgctgc gcgctgagcg acgtgcggca gccgctgcaa caccgcgttg tagatccgcg
     1381 catgctggcg atcgatctgc tttttttgct gtggtcgatg gtagagatta tttacgctgg
     1441 caaacactga attaaacatc agatcggtca atgattgcag cgtaaacacc agcacctgat
     1501 tgtgagaagc ctgacaaatg gcgagatgga aagcgtgatc caactgcgca tgttcgatca
     1561 gcgaaatctc tttgtggttc tcactggcgg cgagcatttt ttcataacag cgggttatca
     1621 caacaaaatc agcctgcgtt cccagcgttg ccgccagcct tgccgattcg ccctccagta
     1681 atgcgcgaac gtcgagcaga tcgtacagcg ttcgcggctg cgtactgaac agatggatca
     1741 gcgggctggt gtcctgctcc cgattaagcc gtgcgacatg agaatcgcga ccctgcgccg
     1801 tttcaataat cccgcgcccg cgcagcacgg tcagcccttc acgcagtgcg gagcgtgaga
     1861 agccgagctt ttcacacagt cgacgttccg agggaagcgg ctgaccgacc ttcagtacgc
     1921 cgtcgataat taaccgttcg atactctctg caaccacttc gcaaataggg cgacgttcat
     1981 ctttcattcc cggacctcgt gcacaggtag gaccaatttt tctgttctta actcgcaaaa
     2041 cacgcacatc acgcaagtga ttatgttaca tcaatttaac attaagttaa ccaagacaag
     2101 gtcacagagc tggaaaaaaa atggtctgac cggtagctaa agagatagac gaaaacgaaa
     2161 agcccgctta ataactgttc acagaagcag cgcgcaaaaa tcagctgcca cacaacacaa
     2221 caaagcgaag cctactcatg agcatcttgt acgaagagcg tcttgatggc gctttacccg
     2281 atgtcgaccg cacatcggta ctgatggcac tgcgtgagca tgtccctgga cttgagatcc
     2341 tgcataccga tgaggagatc attccttacg agtgtgacgg gttgagcgcg tatcgcacgc
     2401 gtccattact ggttgttctg cctaagcaaa tggaacaggt gacagcgatt ctggctgtct
     2461 gccatcgcct gcgtgtaccg gtggtgaccc gtggtgcagg caccgggctt tctggtggcg
     2521 cgctgccgct ggaaaaaggt gtgttgttgg tgatggcgcg ctttaaagag agcctcgaca
     2581 ttaaccccgt tagtcgccgc gcgcgcgtgc agccgggcgt atgtaacctg gcgatctcct
     2641 aggccgttgc gccgcataat ctctattacg cgccggatcc ttcctcacaa atcgcctgtt
     2701 caattggcgg caatgtggcg gaaaatgccg gtggcgtcca ctgcctgaaa tatggtctga
     2761 ccgtacataa cctgctgaaa attgaagtgc aaacgctgga cggcgaggcg ctgacgctgg
     2821 gatcggacgc gctggattca cctggttttg acctgctggc gctgttcacc ggatcggaag
     2881 gtatgctcgg cgtgaccacc gaagtgacgg taaaactgct gccgaagccg cccgtggcgc
     2941 gggttctgtt agccagcttt gactcggtag aaaaagccgg acttgcggtt ggtgacatca
     3001 tcgccaatgg cattatcccc ggcgggctgg agatgatgga taacctgtcg atccgcgcgg
     3061 cggaagattt tattcatgcc ggttatcccg tcgacgccga agcgattttg ttatgcgaac
     3121 tggacggcgt ggagtctgac gtacaggaag actgcgagcg ggttaactac atcttgttga
     3181 aagcgggcgc gactgacgtc cgtttggcac aggacgaagc agagcgcgta cgtttctggg
     3241 ccggtcgcaa aaatgcgttc ccggcggtag gacgtatctc cccggattac tactgcatgg
     3301 atggcaccat cccgcgtcgc gccctgcctg gcgtactgga aggcattgcc cgtttatcgc
     3361 agcaatatga tttacgtgtt gccaacgtct ttcatgccgg agatggcaac atgcacccgt
     3421 taatcctttt cgatgccaac gaacctggtg aatttgcccg cgcggaagag ctgggcggga
     3481 agatcctcga actctgcgtt gaagttggcg gcagcatcag tggggaacat ggcatcgggc
     3541 gagaaaaaat caatcaaatg tgcgcccagt tcaacagcga tgaaatcacg accttccatg
     3601 cggtcaaggc ggcgtttgac cccgatggtt tgctgaaccc tgggaaaaac attcccacgc
     3661 tacaccgctg tgctgaattt ggtgccatgc atgtgcatca cggtcattta ccttttcctg
     3721 aactggagcg cttttgatgc tacgcgagtg tgattacagt caggcgctgc tggagcaggt
     3781 gaatcaggca attagcgata aaacgccgct ggtgattcag ggcagcaata gcaaagcctt
     3841 tttaggtcgc ccagtcaccg ggcaaacgct ggatgtgcgt tgtcatcgcg gcattgttaa
     3901 ttacgacccg accgagctgg tgataaccgc tcgtgccgga acgccgctgg tggcgattga
     3961 agcggcgctg gagagcgccg ggcaaatgct tccctgcgag ccgccgcatt atggtgaaga
     4021 agccacctgg ggcgggatgg tcgcctgcgg gctggcgggg ccgcgtcgcc cgtggagcgg
     4081 ttcggtccgc gattttgtcc tcggcacgcg catcattacc ggcactggaa aacatctgcg
     4141 ttttggtggc gaagtgatga aaaacgttgc cggatacgat ctctcacggt taatggccgg
     4201 aagctatggt tgtcttggcg tgctcactga aatctcaatg aaagtgttac cgcgaccgcg
     4261 tgcctctctg agcctgcgtc gggaaatcag cctgcaagaa gccatgagtg aaatcgccga
     4321 gtggcaactc cagccattac ccattagtgg cttatgttac ttcgacaatg cgttgtggat
     4381 ccgccttgag ggcggcgaag gatcggtaaa agcagcgcgt gaactgctgg gtggcgaaga
     4441 ggttgcgggt cagttctggc agcaattgcg tgaacaacaa ctgccgttct tctcgttacc
     4501 aggtacctta tggcgcattt cattacccag cgatgcgccg atgatggatt tacccggcga
     4561 gcaactgatc gactggggcg gggcgttacg ctggctgaaa tcgacagccg atgacaatca
     4621 aatccatcgc atcgcccgca acgctggcgg tcatgcgacc cgctttagtg ccggagatgg
     4681 tggctttgcc ccgctatcgg ctcctttatt ccgctatcac cagcagctta aacagcagct
     4741 cgacccttgc ggcgtgttta accccggtcg catgtacgcg gaactttgag gagcaggcta
     4801 tgcaaacccg attaaccgaa gagatgcggc agaacgcgcg ctggaagccg acagcatcct
     4861 gtgcgcctgt gttcactgcg gattttgtac cgcaacctgc ccaacctatc agcttctggg
     4921 cgatgaactg gacgggccgc gcgggcgcat ctatctgatt aaacaggtgc tggaaggcaa
     4981 cgaagtcacg cttaaaacac aggagcatct cgatcgctgc ctcacttgcc gcaattgtga
     5041 aaccacctgt ccttctggtg tgcgctatca caatttgctg gatatcgggc gtgatattgt
     5101 cgagcagaaa gtgaaacgcc cactgccgga gcgaatgctg cgcgaaggat tgcgccaggt
     5161 agtgccgcgt ccggcggtct tccgtgcgct gacgcaggta gggctggtgc tgcgaccgtt
     5221 tttaccggaa caggtcagag caaaactgcc tgctgaaacg gtgaaagcta aaccgcgtcc
     5281 gccgctgcgc cataagcgtc gggttttaat gctggaaggc tgcgcccagc ctacgctttc
     5341 gcccaacacc aacgcggcaa ctgcgcgagt gctggatcgt ctggggatca gcgtcatgtc
     5401 agctaacgaa gcaggctgtt gtggcgcggt ggactatcat cttaatgcgc aggagaaagg
     5461 gttggcacgg gcgcgcaata atattgatgc ctggtggccc gcgattgaag caggtgccga
     5521 ggcaattttg caaaccgcca gcggttgcgg cgcgttcgtc aaagagtatg ggcagatgct
     5581 gaaaaacgat gcgttatatg ccgataaagc gcgtcaggtc agtgaactgg cggtcgattt
     5641 agtcgaactt ctgcgcgagg aaccgctgga aaaactggca attcgcggcg ataaaaaact
     5701 ggccttccac tgcccgtgta ccctacaaca tgcgcaaaag ctgaacggcg aagtggaaaa
     5761 agtgttactt cgtcttggat ttaccttaac ggacgttccc gacagccatc tgtgctgcgg
     5821 ttcagcggga acatatgcgt taacgcatcc cgatctggca cgccagctgc gggataacaa
     5881 aatgaatgcg ctggaaagcg gcaaaccgga aatgatcgtc accgccaaca ttggttgcca
     5941 gacgcatctg gcgagcgccg gtcgtacctc tgtgcgtcac tggattgaaa ttgtagaaca
     6001 agcccttgaa aaggaataaa aaaatgaaaa ctaaagtcat tcttagccag caaatggcga
     6061 gtgcaattat tgccgcaggt caggaagagg cgcagaaaaa taactggtct gtttccattg
     6121 ctgttgccga tgacggcggt catctgctgg cgttaagtcg catggacgat tgcgcgccga
     6181 ttgcggctta catctcccag gagaaagcgc gtaccgccgc gctggggcgt cgtgaaacta
     6241 agggctatga agagatggtg aacaacggac gtaccgcgtt cgtgactgcg ccgttactga
     6301 cgtcgctgga aggcggcgta ccggttgttg ttgatgggca aattattggt gccgtgggcg
     6361 tttctggttt aaccggagca caggatgcgc aggtcgcgaa agtggcagca gcggtgttgg
     6421 cgaaataagc gaaaacgagg agagaaacaa tgagtcaaac cataacccag ggccgtttac
     6481 gcattgacgc caattttaaa cgttttgtgg atggagaagt tttgccgggc gtggaactgg
     6541 acgctgctgc attctggcac aatgttgatg agatcgttca cgatctggca ccagaaaatc
     6601 gtcagttgct ggcagaacgc gatcgcattc aggcggcgct tgatgagtgg catcgcggca
     6661 atccggggcc ggtaaaagat aaagcggcct ataaatcttt ccttcgtgaa ctgggctacc
     6721 tggtgccgca accggagcgc gtgacggtgg aaaccacggg cattgacagc gaaatcacca
     6781 gtcaggcggg gccacagctg gtggttccgg caatgaacgc ccgctacgcg ctgaacgcgg
     6841 cgaacgctcg ctggggctca ctgtacgatg cgttatacgg cagcgacatc atcccgcagg
     6901 aaggggcgat ggtcagtggc tacgatccgc agcgcggtga gcaggttatc gcctgggttc
     6961 gacgtttcct cgatgaatca ctaccgctgg aaaacggcag ctatcaggat gtggtggcgt
     7021 ttaaggtggt cgataaacaa ttacgcatcc agttgaaaaa tggtaaagaa accacgttac
     7081 gtactccagc acagtttgtc ggttaccgtg gcgatgccgc tgcgccgacc tgcattttgc
     7141 tgaaaaataa cggcctgcat attgaactgc aaattgatgc caacgggcgg attggcaaag
     7201 acgatccggc gcacatcaac gatgttatcg tcgaagctgc tatcagtacc attctcgact
     7261 gcgaagattc ggtcgcggcg gttgatgcgg aagataaaat cctgctgtac cgcaatctgc
     7321 tgggcctgat gcaggggact ctgcaagaga aaatggagaa aaacggtcgg caaatcgtac
     7381 gtaaactgaa tgacgatcgt cagtacaccg ccgccgatgg ctctgaaatt tctctgcacg
     7441 gacgctcgtt gctgtttatc cgcaacgtgg gtcatttgat gaccattcct gtgatttggg
     7501 acagcgaagg caatgaaatc ccggaaggta ttcttgatgg cgtcatgact ggcgcgattg
     7561 ccctctatga tttaaaagtg cagaaaaact cgcgcactgg cagcgtctat attgtgaaac
     7621 cgaaaatgca cggtccgcag gaagtggcgt tcgccaacaa actgtttacc cgcattgaga
     7681 caatgctcgg tatggcaccg aataccctga aaatgggcat tatggatgaa gaacgccgga
     7741 cctcgctgaa cttgcgtagc tgtatcgctc aggcgcgcaa ccgcgtggcg ttcatcaata
     7801 ccggtttcct cgaccgtacc ggcgatgaaa tgcattcggt gatggaagct ggcccgatgc
     7861 tgcgtaaaaa ccagatgaaa tcgacgccgt ggatcaaagc ctacgaacgt aataacgtgc
     7921 tttccggtct gttctgtggg ctgcgcggta aagcgcaaat cggtaaaggc atgtgggcaa
     7981 tgccggacct gatggcagac atgtacagcc agaagggcga ccaactgcgt gccggggcaa
     8041 acacagcctg ggttccgtca ccaaccgctg ctacgctcca tgcgctgcac taccaccata
     8101 ccaacgtaca gagcgtgcaa gccaacattg cccagaccga gttcagtgct gaatttgaac
     8161 cgctgctgga cgatctgctg actattccgg tggctgaaaa cgctaactgg tcggcgcaag
     8221 agatccaaca agagctggat aacaacgtgc aggggattct ggggtacgtg gtgcgttggg
     8281 tggagcaggg gattggttgt tcaaaagtgc cggatattca caacgtggcg ctgatggaag
     8341 accgcgcaac gctgcgtatc tccagccagc atatcgccaa ctggttacgt cacggtattc
     8401 tgaccaaaga acaggtgcag gcgtcgctgg agaatatggc gaaagtggtt gatcagcaaa
     8461 atgctggcga tccggcttat cgtccgatgg cggggaattt cgctaactcg tgtgctttta
     8521 aagctgccag cgatttaatc ttcctcggcg tgaaacagcc aaacggttat accgaaccgt
     8581 tattacacgc ctggcgttta cgcgaaaaag aaagtcatta atagcgtcaa aaaagccccg
     8641 gcaagtttta ttgtcggggc tatgatagta actgcggtag tacatttatc ccggaaataa
     8701 ccggagcgat tatcagggtt taacaatatt aaatacccgt agcgcattac tagcgggcct
     8761 aaaacaacaa tatggacatc ttaacgatac tctgcgcctg cgtgtgcgga acagaaaccc
     8821 ttacccgata tacttgttat gccagaggta gcaattgcgt tagcggcgta ctgttgctgt
     8881 aaagtataac gcctatattt tacagttcag tcctcttttg cttattacaa ccttaagtat
     8941 atcaagcata taaagataat aagagactga acaatatggt tacctggacc caaatgtata
     9001 tgccgatggg aggactgggg ctatccgctc tggtcgccct gatcccgata atattcttct
     9061 tcgttgcact cgcggtatta cgtctgaaag gacatgtcgc tggagcaata acccttatat
     9121 tatctatcct gattgcgata ttcgccttta aaatgccgat tgatatggca tttgctgctg
     9181 cgggctatgg ctttatttat ggattatggc caattgcgtg gattattgtc gcggcggtgt
     9241 tcctgtataa attaaccgtt gccagcaggc agttcgatat tatccgcagc tcggttatct
     9301 ccatcaccga cgatcagcgt ttgcaggtgt tactgattgg tttctccttt ggtgcgttgc
     9361 tggaaggagc tgctggcttt ggtgcgacgg tggcgtttgg tgcgttgggc gtgccgattc
     9421 tggtcgccgg tcaggtaacg ggaatcgatc cgttccacat tggcgcaatg gcgggacgtc
     9481 agttaccgtt cctgtcggtt cttgtgccgt tctggctggt agcaatgatg gacggctgga
     9541 aaggggtgaa agagacgtgg ccagcggcgc tggttgctgg gggaagcttc gctgtcactc
     9601 agttctttac ctctaactat attggtccgg aactgccgga tattacttcg gcgctggtga
     9661 gtatcgtctc actcgcttta tttcttaaag tctggcggcc gaaaaatacc gaaacggcaa
     9721 tcagcatggg gcaatccgca ggtgcgatgg tggtaaataa gccatcttct ggcggtcccg
     9781 tgccttcaga atatagtctg gggcaaatca ttcgtgcgtg gtcaccgttt ttaatcttaa
     9841 cggtgctggt caccatctgg accatgaagc cgtttaaagc gttatttgct ccgggtggcg
     9901 cgttttattc actggtgatt aatttccaga tccctcattt gcatcaacaa gtgttgaaag
     9961 cggcacccat tgtcgcccaa ccaacgccaa tggatgcggt gtttaaattc gaccctctct
    10021 cggctggcgg caccgctatt tttattgcgg cgattatctc tatcttcatc ctcggcgtgg
    10081 gcatcaagaa agggattggc gtctttgccg aaatgctaat tagtctgaag tggccgatat
    10141 tgtcgattgg catggtgctg gcattcgcct tcgtcaccaa ctattccggc atgtccacca
    10201 cgctggcgct ggtactggca ggtacaggcg tgatgttccc gtttttctca ccgttttctc
    10261 ggctggctgg gcgtattcct taccgggtcg gacacttctt caaacgccct gtttggttca
    10321 ctgcaatcga ccacggcgca gcaaatcaac gtctctgaca ctctgctggt ggcagcaaat
    10381 accagcggcg gcgtaactgg caagatgatc tccccgcaat ctatcgccgt ggcctgcgcc
    10441 gcgacgggca tggtgggccg agaatctgaa ctgttccgct acaccgtgaa gcacagtctg
    10501 atttttgcca gcgttatcgg cgttatcacc ctgctgcagg cgtatgtgtt taccgggatg
    10561 ttagtctcgt aaatcaataa agccggatga aggtaaatat cacccggctt tccatttgtc
    10621 tgtcgtttat ctcgccaggg aaccgttttt acgattggct tctttatacg tagtctccgt
    10681 tttacgatgc gaattgttta atatttatac aacttggctt ctgtgctggt tataaagtac
    10741 ccactttcat gagatcaatt ttagtatgtt attacattgc ttcatctgtt gctttcatga
    10801 cgaatggcgt tttttctctt atttatattt ttcactagaa caatgagtag atcaaaattc
    10861 ccaaaactcc caaaaacata agattattcg caaagctgat tttgcagaat aatgagaact
    10921 tttaagattt gtcgaggatc aattttaagc aatttatctc cacttgttac ataaaagatg
    10981 ccgttaggtt aataagttaa tcttgctata acagtgttcg ttactgccta ttaataaaga
    11041 tatttgggat agggtaacgt ggatgaggat tcgaaatcat ccgtcttgcg gaaagtgaaa
    11101 tgttggcaga tgttaatatt atgtatgtat tttaactcca acaacacacc aatcattttg
    11161 ttagaaataa gaaaatttat ggttaaataa tttggtgatt atgaagcatt aaatcgtaat
    11221 gccaaatatt gttcgctttg gagagtatta ttgcctgttt gtgtctgttg agcagaaaaa
    11281 tcatctgaat ttgaatactt ttattgggtg tgaataaatg aaaaaatcat taaaatcatt
    11341 aaaatcatta aaatcattaa aatcattaaa atcattaaaa tcattaactt atatgaatat
    11401 ttcagagttg gagttcgaat gttgacacct gagtatttcc tgctaaaaat agccctgctt
    11461 tacggatggt tgtattgcaa taacacgaaa gtagtaagag attaaaggta tctgtcagcg
    11521 ataactctgc aagtgatgac attttgtaaa ccaaataatg taaatcagca atgtatcttt
    11581 tcacttaatt ataacaaatg tggtattcac taagaatctg atacgagctt acatcacgac
    11641 tattttagtc tcatcgcggg atacagggat gcaccgatgg atctcagcac accgtaatgc
    11701 aatacctgaa caaatagaac agtaaagtgg ttcagtatta atgaaagctc ttattttccg
    11761 ttgggaaaac ttaaaataga tgagaaggct ttttgatttc ttatgtgatc tcatttcgag
    11821 tattgagaaa tttgtgattt ttaaattttt caataagccc tgtaagggat tggggtttta
    11881 tgagaaataa agagacgtat tgtctttgtt taaacggtac g
//
LOCUS       DAOFMX010000114        11783 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.114, whole genome shotgun sequence.
ACCESSION   DAOFMX010000114 DAOFMX010000000
VERSION     DAOFMX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.114"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..2341)
                     /locus_tag="QBA20_003653"
     CDS             complement(<1..2341)
                     /locus_tag="QBA20_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDQ6294045.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEEMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="QBA20_003654"
     CDS             complement(2275..2898)
                     /locus_tag="QBA20_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDQ6294046.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="QBA20_003655"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="QBA20_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="QBA20_003656"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="QBA20_003656"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDQ6294047.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /locus_tag="QBA20_003657"
     CDS             7506..8228
                     /locus_tag="QBA20_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDQ6294048.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..10290)
                     /gene="yfaL"
                     /locus_tag="QBA20_003658"
     CDS             complement(8356..10290)
                     /gene="yfaL"
                     /locus_tag="QBA20_003658"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDQ6294049.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10300..11754)
                     /locus_tag="QBA20_003659"
     CDS             complement(10300..11754)
                     /locus_tag="QBA20_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HDQ6294050.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSVLDLSDFSTWQSGT
                     SYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNS
                     YLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2727 a         3256 c         2910 g         2890 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagaccgt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatctcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc attgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tcaggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccggcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag ctatccagcc aggccccctg
     8881 cttctgatta ccgtgctgat accagcttga ggtcagccca acggcataac cgtggttctg
     8941 gttatcggcg cgagttccgg tcatattcga gcggctgtcg ccctgattat cactgtagcc
     9001 accaaccgcc ccaagcatcc actcgccatc cgtgccccag cgcccgctaa acaagccgcc
     9061 gctaagctgc accgtagaag tgtcttcctg ctgagccagt tgccctgccg ccgtgtaatg
     9121 ataacgtccg ccgataacac gtaaattcag cgtctgacca tcgccaccag cgtgatcgcg
     9181 tcgctccatc ataaacgcct gatttgccgc ccgcaggtta ttgagataac cgccaacttt
     9241 ggcattcagc accggctggt aagcaggcgt aggggccggg tcgggggttg ggtcaggatc
     9301 cggcgtggga tcaggatcgg gagtcgggtc tggctcaggt ggcgatggcg gcgttacttc
     9361 ttgcgatcgc agataccagt cgttgttatc ttccactagc gtgtagtcat acgctcccat
     9421 gttgacgtag ccactgccag ccagactgaa ctgtgcattg ttttgaaact gcgtgggatc
     9481 agctgcgaaa tcaaccactt taatgcctgt cgatgtcggc tcaccaatcc ctgtaatgga
     9541 gttaaccacc acagttgtgt tgccagcagt attaccgttc atcaccaatt gatcgcttac
     9601 cgagtcatcg ccgtttaatt cgctgtcgaa tagtagcgta ccgccgccgg tataatcacc
     9661 gtttaccgtc agcgtatcgc cagcgacgcc attttgcagg ctaaccgcac cggacgtatt
     9721 ggtcagatca ccattaaccg tcacgtcagc aagataggtc aggctgtcag tttcaaggtt
     9781 ggtattaagc tcgcctgtca acgtcacgcc atcggtagca tttaccagcg tcccatcgcc
     9841 gttaaatagt gaggcattaa gggcgacgga agtatcctgc ccggtcagac gcaaaaccgt
     9901 accatcgctg atgtcgatag tgccgctgga agtagcatca attgactgaa tatcctgatc
     9961 cgcgccagta acgaacgttg ccccatcacc aacccacagt gacgaagcat aaggaaagat
    10021 atccgcaaca tcgcctttca gggtgccttc ttcgacacgt accgccgact gcgttgtacc
    10081 gctggcggtc agctccagcg tacccgcccc cgttttagta aatgtgctac cctcatcctg
    10141 atgctgcccg ctgctgtcag ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac
    10201 tgccacttca ccgtcggcgc gcatttcaat atcacgccca tgctcagtcg tcgtagagcg
    10261 agtatcgaca ttggcagtaa tctccatcac gctttcttgt tacttatcgg taaagataac
    10321 ctggcggtta tagtgggtat ctccaagctg cgagttgtcg ctcaccatca gcgtaccgga
    10381 ggcgatttgt gttgtgccca aataactatt attgtttgcc agacttacct gcccgtcgct
    10441 cgcatcgacc accacgtaga cgccatcttt cccgtcgccg ccgatatgca aattgtcgcc
    10501 acctgcaaga tctaccacat cctgactgcc gataaccgtt ccgctgctgc cactgacttc
    10561 aaggccatca ttgtatgatg tgccgctctg ccaagtggag aaatcagaga gatcgagcac
    10621 actgccgttt tgcgcaatgg tgagctgacc agcaccttcg atgatgccag caaaactgcc
    10681 ctgattaaca gtaacgttgc caccagcatc gatatttaaa gtgccattct gaaagcccgt
    10741 caatgcgtgc acaaaagttt gttgggtcga gccaacgttt agctcagcct gattctgata
    10801 ctgatcaata ctccctatcg tcagaccgta gcagtcttgc ggatcgtcct ggcaatgcgt
    10861 atcgccgaca ttcatcaggg agttgctgcg acccagggta acttcaccgt tttcaatctg
    10921 catctcacca gtaaagtcat tgttatctgc attaagtacc agatcgccgg aacctgtttt
    10981 ggtgattaac ccggtaccag caatagagtc aacagctccg tcattctctg tattgccgat
    11041 aaccagcgtt tttccgtcgg caatatcaaa ggtaacttca cttaagccga gatacataaa
    11101 gccacccgcc gcagtggagg gaccatctcc atagcctgct gcgctattgt tctcatcgac
    11161 taacacgccg ccgttctggc tgtagctgtc atcaacagaa atatcaataa gatagggagc
    11221 cgtcgcgcta ttggtatata tcgccccgcc ataaccttcg gctgtgttat ttgtaaaggc
    11281 ggtgttattt attatcgtat aacctgaagg atgcttgctg tcgctattat tatcggtaac
    11341 atcgattgcc ccgccatcgc catcactgta acttgttgat gtatatgcct ggttattgtt
    11401 aaaaacatca tcacttaaat agacgtcatt attaatggta taaattgcgc cacctttgcc
    11461 atcattagcg atattattga tggcgttagt gacacgtaaa tcgacggcac cagtatcatt
    11521 agtaccagaa gaatagattg cgccaccata gccgcctgcg acgttaccgg aaaaaataac
    11581 atcagtaaga ttcagcgttg agttttcttt agcaaatatg gccccgccgt tattatattc
    11641 tcctgtaacg gtgttattgg caaacagagt cattccagtt tcattttctg gtaatagtga
    11701 aaattccgct ccttgttgca aaaatacggc cccaccgctg gcattattgg tcatatccga
    11761 aaaaaccagc cattgcccat cgg
//
LOCUS       DAOFMX010000115        11668 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.115, whole genome shotgun sequence.
ACCESSION   DAOFMX010000115 DAOFMX010000000
VERSION     DAOFMX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.115"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            597..794
                     /locus_tag="QBA20_003660"
     CDS             597..794
                     /locus_tag="QBA20_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294051.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="QBA20_003661"
     CDS             complement(744..1937)
                     /locus_tag="QBA20_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6294052.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="QBA20_003662"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="QBA20_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HDQ6294053.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="QBA20_003663"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="QBA20_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HDQ6294054.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="QBA20_003664"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="QBA20_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HDQ6294055.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="QBA20_003665"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="QBA20_003665"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HDQ6294056.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="QBA20_003666"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="QBA20_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HDQ6294057.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="QBA20_003667"
     CDS             complement(10573..10716)
                     /locus_tag="QBA20_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294058.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10966..>11668
                     /locus_tag="QBA20_003668"
     CDS             10966..>11668
                     /locus_tag="QBA20_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ6294059.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWD"
BASE COUNT         2960 a         2900 c         2994 g         2814 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattaa
    10801 cgatcaatta gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt
    10861 tacaatatag aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt
    10921 aattcttaat actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac
    10981 tcccttaaat atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga
    11041 gtttctggta aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt
    11101 acagtaaatg cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt
    11161 gcacaaaata aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac
    11221 ggagataaat tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt
    11281 ggcgcagcta ccgcaatagg aggttcttat agtgttactg ttgcacataa caaaaagaac
    11341 cctcaggccg cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga
    11401 aattccaatg attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc
    11461 accccggcag agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc
    11521 acttctgacg gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt
    11581 attaatggtg aatccaaaat ctcaacaaat tcagcatata gccatgacct gttaagtgct
    11641 agtctatttg aggtcaccca atgggact
//
LOCUS       DAOFMX010000116        11631 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.116, whole genome shotgun sequence.
ACCESSION   DAOFMX010000116 DAOFMX010000000
VERSION     DAOFMX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.116"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(282..357)
                     /locus_tag="QBA20_003669"
     tRNA            complement(282..357)
                     /locus_tag="QBA20_003669"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3168
                     /locus_tag="QBA20_003670"
     CDS             916..3168
                     /locus_tag="QBA20_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294060.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3504..4481
                     /gene="csiD"
                     /locus_tag="QBA20_003671"
                     /pseudo
     CDS             3504..4481
                     /gene="csiD"
                     /locus_tag="QBA20_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
     gene            4501..5769
                     /gene="lhgO"
                     /locus_tag="QBA20_003672"
     CDS             4501..5769
                     /gene="lhgO"
                     /locus_tag="QBA20_003672"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDQ6294061.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5792..7240
                     /gene="gabD"
                     /locus_tag="QBA20_003673"
                     /pseudo
     CDS             5792..7240
                     /gene="gabD"
                     /locus_tag="QBA20_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7248..8534
                     /gene="gabT"
                     /locus_tag="QBA20_003674"
     CDS             7248..8534
                     /gene="gabT"
                     /locus_tag="QBA20_003674"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDQ6294062.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8772..10172
                     /gene="gabP"
                     /locus_tag="QBA20_003675"
     CDS             8772..10172
                     /gene="gabP"
                     /locus_tag="QBA20_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDQ6294063.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10193..10855
                     /gene="csiR"
                     /locus_tag="QBA20_003676"
     CDS             10193..10855
                     /gene="csiR"
                     /locus_tag="QBA20_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDQ6294064.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="QBA20_003677"
     CDS             complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="QBA20_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDQ6294065.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11389..11547)
                     /locus_tag="QBA20_003678"
     CDS             complement(11389..11547)
                     /locus_tag="QBA20_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDQ6294066.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2991 a         2855 c         2902 g         2883 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 ggggggagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg tccacgcgtg tcggtggtga ggcgtggctt
     2761 aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat
     2821 aaagcaggaa tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt
     2881 agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat
     2941 aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa
     3001 aacatgggga gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct
     3061 tgtaaagtat ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct
     3121 cttcttttag ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata
     3181 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg
     3241 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
     3301 cctgttgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
     3361 tactaatttg ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt
     3421 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
     3481 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
     3541 attcaggcta ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
     3601 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
     3661 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg
     3721 atctgtgtgc gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
     3781 gtgcgctgtt gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga
     3841 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
     3901 agtattacgc gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc
     3961 cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg
     4021 tgctgatgat gaaaatcgat gagcaaaaca tgcagggtgg aaattcgttg ctgctgcatc
     4081 tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt cgcccgatgc
     4141 gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca gtgttcgacg
     4201 ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag ccaaaagatt
     4261 ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc aaaggcattc
     4321 tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc tggctgcacg
     4381 gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt cagcgtggct
     4441 atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg aattgagcgg
     4501 atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc catgcaactg
     4561 attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg cccggcctgt
     4621 caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac gcccggcagc
     4681 ctgaaggcgc agttttgcct ggcggggaac cgtgccacca aagccttttg cgatcaaaac
     4741 ggcattcgct atgacaactg cggcaagatg ctggtcgcca cgtctcagct cgaaatggaa
     4801 cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga gtggataaac
     4861 gccgtggaac tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat ttttgtgccg
     4921 tccagcggca ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat cttccaggcc
     4981 agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca taaaaacggc
     5041 gtggtgatac gcacccgtca gggcggtgaa tatgaagcat cgacgctgat tagctgttcc
     5101 gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt tattatctgc
     5161 ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt taaccacctg
     5221 atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac ccgcatgatc
     5281 gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga aggctatcgc
     5341 aagcgcgact tctcatttag tgacacgctg gagattttga gctcgtcggg gattcgccgg
     5401 gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct gtgcaaaagc
     5461 ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga tctccagccc
     5521 tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat tgacgatttt
     5581 ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc ggcagcgaca
     5641 tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt ggcaagccag
     5701 agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca cgccgcattt
     5761 aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta acttattccg
     5821 ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag tcatcgacgt
     5881 caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg cagatgaaac
     5941 ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc tcaccgccaa
     6001 agaacgcgcc accattctgc gcaactggtt caatttgatg atgcagcatc aggacgattt
     6061 agcgcgcctg atgaccctcg aacagggtaa accgctggct gaagcgaaag gcgaaatcag
     6121 ctacgccgcc tccttcattg agtggtttgc tgaagaaggc aaacgtattt atggcgacac
     6181 cattcctggt catcaggccg ataagcgcct gattgttatc aagcagccga ttggcgtcac
     6241 cgccgccatc acgccgtgga actttccggc ggcgatgatt acccgtaaag ccggtccggc
     6301 gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt tctctgcgct
     6361 ggcgctggcg gaactggcga ttcgcgcggg cattccggct ggcgtgttta acgtggtcac
     6421 cggttcggca ggcgcggtcg gtaatgaact gaccagcaac ccgctggtac gcaaactgtc
     6481 gtttaccggt tagaccgaga ttggccgcca gttaatggaa cagtgcgcga aagacatcaa
     6541 aaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc gtctttgacg atgccgatct
     6601 cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc aaacctgcgt
     6661 ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg aaaaattaca
     6721 gcaggcggtg agcaaactgc acatcggcga cgggctggag aaaggcgtca ccatcgggcc
     6781 gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg cgctggagaa
     6841 aggtgcgcgt gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact tcttccagcc
     6901 aaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga cgttcggccc
     6961 cctcgcccca ctgttccgtt ttaaagatga agccgatgtg attgcacaag ccaatgacac
     7021 cgagtttggt cttgccgcct atttctacgc ccgtgattta agccgcgtct tccgcgtcgg
     7081 cgaggcgctg gagtacggca tcgtcggcat caataccggc attatttcca atgaagtggc
     7141 cccattcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt atggcatcga
     7201 agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg cgaaggaaca
     7261 gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt gggcaaattc
     7321 acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgttgaa ggccgtgagt
     7381 atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat ccgaaggtgg
     7441 tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag gtgctggctt
     7501 acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc gatttcgcca
     7561 agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta aaaatcgccc
     7621 gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgagtatcac gggcgcacgc
     7681 attacaccct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg gggctgatgc
     7741 cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc gaggatgacg
     7801 ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat atcgccgcca
     7861 tcatgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca gcctttatgc
     7921 agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat gaagtgcaga
     7981 gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtg gcgccggatc
     8041 ttaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc accgggcgcg
     8101 cggaagtgat ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc ggtaacccga
     8161 ttgcctgcgt ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat ctgctgcaaa
     8221 aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc gaaaaacacc
     8281 cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg tttgaagatg
     8341 gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc gcccgcgata
     8401 agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc cttgtaccac
     8461 tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag tgttttgatg
     8521 aggcgaagca gtagcaccgc ccccatgcag gaggcgacgc tgcgcgtctt gtccggccta
     8581 cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac gtgaacagga
     8641 ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa tcaataacaa
     8701 ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga
     8761 gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg ctgaaatcac
     8821 gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca
     8881 gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc gccggattac
     8941 tggtggttat gattatgcgg atgctggcgg aaatggcagt tgccacgccc gataccggtt
     9001 cgttttccac ctatgccgat aaagccattg gccgctgggc gggctatacc atcggctggt
     9061 tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc gccatgatcc
     9121 tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt accctcgcct
     9181 taactggcag taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc
     9241 tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt
     9301 tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc
     9361 ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg tttatgggcg
     9421 cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat attgtccgcg
     9481 ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt tttgtcgtcg
     9541 tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat cgctcggttc
     9601 tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata ttactttccg
     9661 taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac tccttaagcc
     9721 gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc ccgtacgtgg
     9781 cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat tacgcgcctg
     9841 cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg gtttatttag
     9901 tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga agcgaaattc
     9961 gcttgcgaat gtggcttgat ccctggctca cctggctggt catcggcttt attacctttg
    10021 tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc accggcttat
    10081 tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag ctggtattgt
    10141 ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca ccatgaccat
    10201 tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc
    10261 ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg ttgggccgtt
    10321 gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg
    10381 gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg cccgcgccaa
    10441 tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga
    10501 cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat
    10561 gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg
    10621 ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gttatcgatt
    10681 tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac acgatcagca
    10741 ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg agttaatgcg
    10801 ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact
    10861 cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt
    10921 tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg
    10981 cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg
    11041 ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt
    11101 tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt
    11161 gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct
    11221 tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt tctccggcat
    11281 ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag
    11341 tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt aatcgcgggt
    11401 ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt
    11461 aataatgaac gcccaaccga accctttacc gagcagcacg ccgatcggcg gcagaatgat
    11521 ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt
    11581 aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag t
//
LOCUS       DAOFMX010000117        10963 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.117, whole genome shotgun sequence.
ACCESSION   DAOFMX010000117 DAOFMX010000000
VERSION     DAOFMX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.117"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..241
                     /locus_tag="QBA20_003679"
     CDS             <1..241
                     /locus_tag="QBA20_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294067.1"
                     /translation="NGSVRYCQYTSMFGASYRYSGAGVGYQVASVVGGGFTPFIAAAL
                     ITYFAGNWHSIAIYLLAGCLISAMTALLMKDNQRS"
     gene            343..1797
                     /gene="amn"
                     /locus_tag="QBA20_003680"
     CDS             343..1797
                     /gene="amn"
                     /locus_tag="QBA20_003680"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDQ6294068.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2140..2856
                     /locus_tag="QBA20_003681"
     CDS             2140..2856
                     /locus_tag="QBA20_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDQ6294069.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3312..3387)
                     /locus_tag="QBA20_003682"
     tRNA            complement(3312..3387)
                     /locus_tag="QBA20_003682"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3352..3354),aa:Asn,seq:gtt)
     gene            complement(3488..5115)
                     /gene="yeeO"
                     /locus_tag="QBA20_003683"
                     /pseudo
     CDS             complement(3488..5115)
                     /gene="yeeO"
                     /locus_tag="QBA20_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5120..5195
                     /locus_tag="QBA20_003684"
     tRNA            5120..5195
                     /locus_tag="QBA20_003684"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5153..5155,aa:Asn,seq:gtt)
     gene            complement(5233..6183)
                     /gene="cbl"
                     /locus_tag="QBA20_003685"
     CDS             complement(5233..6183)
                     /gene="cbl"
                     /locus_tag="QBA20_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDQ6294070.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6285..7202)
                     /gene="nac"
                     /locus_tag="QBA20_003686"
     CDS             complement(6285..7202)
                     /gene="nac"
                     /locus_tag="QBA20_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDQ6294071.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7530..7605
                     /locus_tag="QBA20_003687"
     tRNA            7530..7605
                     /locus_tag="QBA20_003687"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7563..7565,aa:Asn,seq:gtt)
     gene            complement(7661..8596)
                     /gene="ldtA"
                     /locus_tag="QBA20_003688"
     CDS             complement(7661..8596)
                     /gene="ldtA"
                     /locus_tag="QBA20_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDQ6294072.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8658..9737)
                     /gene="cobT"
                     /locus_tag="QBA20_003689"
     CDS             complement(8658..9737)
                     /gene="cobT"
                     /locus_tag="QBA20_003689"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDQ6294073.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9749..10492)
                     /gene="cobS"
                     /locus_tag="QBA20_003690"
     CDS             complement(9749..10492)
                     /gene="cobS"
                     /locus_tag="QBA20_003690"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDQ6294074.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10489..>10963)
                     /gene="cobU"
                     /locus_tag="QBA20_003691"
     CDS             complement(10489..>10963)
                     /gene="cobU"
                     /locus_tag="QBA20_003691"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDQ6294075.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3045 a         2790 c         2374 g         2754 t
ORIGIN      
        1 aaatggtagt gtccgctatt gccagtacac ctcaatgttt ggtgccagtt atcgctacag
       61 tggtgctgga gtcggttatc aggttgccag tgtggttggc ggtggattta caccttttat
      121 tgccgctgca ctcatcactt actttgccgg gaactggcat agcatcgcca tttatttgct
      181 ggctggatgc ctgatttctg caatgaccgc tttattgatg aaagacaatc aacgctcgtg
      241 atagcctggc gaagatcatc cgatcttcgc cttatacttt tgtttcacat tcctgtgaca
      301 tactatcgga tgtgcggtaa ttgtatggaa caggagacac acatgaataa taagggctcc
      361 ggtctgaccc cagctcaggc actggataaa ctcgacgcgc tgtatgagca atctgtagtt
      421 gctttacgca acgccattgg caaatatatt acaattggcg aattacctga tgaaaacgcc
      481 cgtaaacaag gtctttttgt ctatccatca ctgaccgtaa cctgggacgg tagcacaaca
      541 aatcccccca aaacgcgcgc atttggtcgt tttactcacg caggcagcta caccaccacg
      601 attactcgcc ctactctctt tcgttcgtat ctgaatgagc aactaacgtt gctttatcag
      661 gattatggtg cgcatatctc tgtgcaaccc tcgcaacatg aaatccctta tccttatgtc
      721 atcgatggct ctgaattgac acttgatcgc tcaatgagcg ctgatttaac tcgctacttc
      781 ccgacaacag aactggcgca aatcggcgat gaaactgcag acggcattta tcatccaacg
      841 gaattctccc cgctatcgca ttttgatgcg cgccgcgtcg atttttccct cgcacggttg
      901 cgccattata ccggtacgcc agtagaacat tttcaaccgt tcgtcttgtt taccaactac
      961 acacgttatg tggatgaatt cgttcgttgg ggatgcagcc agatcctcga tcctgatagt
     1021 ccctacattg ccctttcttg tgctggcggg aactggatca ctgccgaaac cgaagcgcca
     1081 gaagaagcca tttccgacct tgcatggaaa aaacaccaga tgccagcatg gcatttaatt
     1141 accgctgatg gtcagggtat tactctggtg aatattggcg tgggaccgtc caatgctaaa
     1201 accatctgcg atcatctggc agtgctacgc ccggatatct ggttgatgat tggtcactgt
     1261 ggcggattac gtgaaagcca ggccattgga gattatgtcc ttgcacacgc ttatttacgc
     1321 gatgaccacg ttcttgatgc ggttctgccg cccgatatac ctattccgag cattgctgaa
     1381 gtgcaacgtg cgctttatga cgctaccaag ttagtcagcg gcaggcctgg tgaggaagtc
     1441 aaacagcggc tgcgtacagg tactgtggta accacagatg acaggaactg ggagttacgt
     1501 tactcagctt ctgcacttcg ttttaactta agccgggccg tagcaattga tatggaaagt
     1561 gcaaccattg ccgcgcaagg atatcgtttc cgcgtgccat acgggacact actgtgtgtt
     1621 tcagataaac cgttacatgg tgagattaag cttcccggcc aggctaaccg tttttatgaa
     1681 ggcgctattt ccgaacacct gcaaattggc attcgggcga tcgatttgct gcgcgcagaa
     1741 ggcgaccgac tgcattcacg taaattacga acctttaatg agccgccgtt ccgataataa
     1801 gttatgaacc ttcttatcag aaatagatgt gaaggattaa gtaagacatc tggcaaatag
     1861 cctgcaataa acgcgggctg cttgcttcct tgggcagatg cgagttttat tatcgtctta
     1921 atgatttcca catattaaaa gcaagtatgc tttcaaaaca caattataaa aaatcccgcc
     1981 aacaacatta gtttttataa aattaaatat aagattatgg ctttagaata tttttatttc
     2041 taatagacga gatttttcct gttatgatat aatatgccga attaacacat gttaacgatt
     2101 taccagtaat gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat
     2161 attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt
     2221 gtagaaatct atgctgctgc caaacaaggt gaacccgatc cagaattaaa cacatcttta
     2281 aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca
     2341 attgataaag ccaaaggcgg cggagatgaa acgttcgtac agggacgtta tgaaggcttt
     2401 ggtcctaatg gctcaatgat tattgctgag acattgactt caaatgttaa ccgtacgatt
     2461 gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc
     2521 agctatatgt ttgacaatac gggtgtgatt gtatttaaag gaacagaccc tgaccatatt
     2581 tttgaaattt tgcttgaagc tgaagttgat gttcgtgatg taactgaaga agaaggtaat
     2641 attgttattt atactgaacc tgctgacctt cataaaggaa tcgcggcgct aaaagcagct
     2701 ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt
     2761 tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat
     2821 gtacaaaaag tttatcataa cgtcgcaaat ctctaattat attttaaaga aatctgtctt
     2881 tatggcagat ttctttaatc tcatataatt cttataaaaa atataatatt taactcgtca
     2941 tattgcttat aaaaaatccc cgtccccaga gaaataatat ttattaaaat ggcaggtctt
     3001 ctttttctga ttaccgaaga caaagtggca attacgtata gtttcccgat aaagacgcga
     3061 cagctacgtc ccgcataagg catttttctc tttatctttg tacggtactt catggaacag
     3121 aatttttgac cttgtgaatc gtgatatctg ttggggaggg gcaatttgct cactgaagcg
     3181 tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggccact tttttacaaa
     3241 gtggccgggt aaaaataatt tttatttttt aactgtcctg agtctcatag agatgtctca
     3301 aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat taacagtccg
     3361 ccgttctacc gactgaacta cagaggaatc gtgagaacga ggcgaatatt agcgatgccc
     3421 acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat aatctgcatt
     3481 ttgtcgttta ttccgacaca actggctttt tttcacactt ttgcggctcg gatcgagggt
     3541 atttccacag ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc cgcacagccc
     3601 agtcggcaaa cattcccatc cagacaccaa ccacacccca gccaagcatg attccctgca
     3661 cataaccgac tacaacccgg caaccccaca tgctcaacat agaaacccac atggcgtaac
     3721 gggcatcacg agcaccctta aatccagcgg gtagcaccca tgaggcggac caaataggca
     3781 taaataaagc atttagctaa atcagaatca caacgacgtg tttaacctgt ggatcctggg
     3841 tgtaaaacga tgccataacc ccggcaaagg gagccgttag ccaggcgatg gccgttaatc
     3901 caagagtgga cagccagaac acatgccgca actgaatctc tgcttgcgct atctgcccta
     3961 cccccaacct tcggcctgta atgatcgtag aagcagaacc gagcgcactt ccgggtaagt
     4021 tgataagagc cgcaattgag aacgcgataa aatttccggc aataacgctg gtccccatcc
     4081 cggcaacgaa catttgggtt aataaccgac cactggtaaa taacactgat tcgacactcg
     4141 ctggaatacc aatccccatg acttcccaga taatgctaaa attcagcggt ttaaaatagc
     4201 tctttaacga aatccttagc gcaggattaa aaccaatcgc cagcacccac aaaattgcaa
     4261 ctgcgccaat ataacgagaa atggttaaac ccaacccagc cccgaccagg agaaaaggcc
     4321 gtaaatcaat atgccgctaa taataatatt aagaatattc aggctaccgt taatcagtag
     4381 cggtattttc gtattccctg caccacgaag tgccccgcta ccaataagag tgatggcagc
     4441 ggctggataa ctgagtacgg tcagctccag ataagtcaac gccagtgctt taacttctgt
     4501 cgtggcatca cccgcgacga aatcaataat ttgttcgcca aaatgatgaa ttagcgttgc
     4561 caacagtacg gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca ccctcgctcg
     4621 tcgtcgatcc cgcttaccga gactaaatgc tacaacgaca gtagtaccaa gatcgatagc
     4681 tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc aaccccacgc cggccatcgc
     4741 atcttttccc agccaactga ccagaaaagt gctcaggacc cccatcaaca ggacacaggc
     4801 gttctccatg aagataggaa cagcaagcgg ggttatctcg cgctagaaca acactttgta
     4861 gctcttgcgt ttagagtgcc agcgagtgcc gtgaacaacc tggcgtaaag cagaggagat
     4921 attcaaagcc gaccttaatt gcagaaagtg aaaccacatt tcaaatgatg agggagaatc
     4981 agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa atgcaacaaa ctgttgatag
     5041 aaacggcaaa cagttgggaa atttaaaaat cgggtttgac aaaatatttt tcgccgttaa
     5101 gatgtgcctc aacagcgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc
     5161 gtatgtcact ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc cgcttttata
     5221 gcgggctttt tgctatatct gataatcaat ttcctcttca ctgttttcca tcacctggcg
     5281 cttgatatcc tcaactgaca gtcctgcatt acaaagttcc agaaagcgcc aaacatagtt
     5341 acgctgaagt tgtcctcgct tcagtcccaa ccagacagta ttagcatcga aaagatgccg
     5401 cgtatccagg cggattaaat tctcttcctc ttgttcgccg ctggattgtt cggcaactaa
     5461 tccgatccca agcccaagag caacataggt tttaatgaca tcagaatcct gtgcacttaa
     5521 tacaatatct gccggcaaac ctttgcgggt aaatgcgtca tcagtgcgtg agcgccccgt
     5581 aattccctgt cggtaagtaa ttaacggcca cttcgctatt gattccagcg tcaatggtgt
     5641 aatttgcgtt aagggatgat cgagtggaac aagcaaacta tggtgccaac aaaaccacgg
     5701 gaaggcgacg agctgcgggt cattactcaa acgctcgctg gcgataccaa tatcagcttc
     5761 gccattttgc aacaacgtcg caatttcctg tggcgaaccc tggattagct cgagccgaac
     5821 ctccgggaaa agttcgcgaa aagctttaat gacctctggc aagctataac gtgcctgagt
     5881 atgcgtcgtt gcaatagtga gaacaccaga cgtatcgttg gtaaacaggt ctgcaagccg
     5941 acgaacatta ctggcttcat tcagaatacg ttctgcaatg accagtaatg ctttgcccgg
     6001 ttcagtcatc cccagcagtc gcttacctcg tcgaacaaat atttcgatgc caaattcatc
     6061 ctccagttcc cgaatatgac ggctaacgcc tgactgtgag gtaaaaagca tattcgcaac
     6121 ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt atctttagtt gttggaaatt
     6181 cacggtaaac tccgggcagt tcagatatcc cgttattgtt aaagtctaat gcccggcata
     6241 acaaataata aaaacccgca tcttattcca tcccgatata acgcttagct caccaattgc
     6301 cactgccttt tttccatcac tggagaacta atcactgaca ttaacaactc tttcactgcc
     6361 tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta aggacaaaga gagactcatt
     6421 gaaggagtgg taatgcgtga catccaccca tttactgcgc cacataacga acgcgcagcc
     6481 gattcaggta atactgcaac gcccatgccg ctggcaatcg ctgcggtaag cgtggcaata
     6541 gactcaattt caccaatgac ttttgccgtg agccgccgta gggaaaaagc ctcatcaaca
     6601 cgaagtctaa cagcgctgta atcactgggg agaaaaaggt tcatttgcgc aatagcatta
     6661 acatcaacgt tttgccccgg gcaatcttga gttcctacca ggaaaagatc ttctttcagc
     6721 aaagcctgac tggatacccc agccacaggg gaatgctcat aaatcaccgc catatcgagt
     6781 tggtgattta tcagtttttc gttaagcact gcaccactat tttcatgaag atagataacg
     6841 acctccggga attcagcgcg aaccgcctgt aataagggca tggtgatgga tgacgcagcg
     6901 gttcctggcg caaagccaat cgagacttgc cccgataatg actgaccaac gttatgcacc
     6961 gccagttggg cctgctcaca ctgacgtaaa atggcccgtg catgggtata gagaattttt
     7021 ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca aaagctgttg atttaactca
     7081 ccttccagta tggcaacctg ctggctgagc gctggttgtg cgatatgcaa tacttcagca
     7141 gcctgggtca ggctaccaat atctacaatt tttacgaagt atttcaggcg tctgaagttc
     7201 atgttgcctc cggtttttaa gaatcggccc aagtgccgcc attacttaca accagtatgc
     7261 aagatgcctg ccagttctat tttggcgttg atgcgcaagc taaccaactg tcaaataaga
     7321 gattatgata gattcgtcat tggccctttt tatcagcagt cgcattcacc tgccctataa
     7381 aaggagggta tgcaccacga tggttcatta cccaataaga ttgaaagctc accactttgt
     7441 tgaaattggc agcaaacaaa caaaaaaaat gcatttcacc ctttgacatc accatgcact
     7501 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     7561 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     7621 gctttcgagc aggcttttta ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     7681 cagaactaat ttgcacaggc attcccgatc gatgttgcaa cgcagcattt gcgcgattta
     7741 catcaacttc ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     7801 ttcggtcaga ctcatattct gcacgattgc gcgacagcgg ctcatgaact tccagccact
     7861 ttgaaccatc cggttccgtt gtgtatttca ctggctgatc aataatttgt acacgagtgc
     7921 ctacaggaac attatcaaac agatatttga tatcgtcatt acgcagacga atacagccct
     7981 gacttacacg gagcccaata ccaaaattgg cattggtacc gtggatggca tacaacctgc
     8041 caatataaat cgcgtacagc cccatggggt tatcgggccc cgcaggaaca aatgcgggca
     8101 aactctcccc tcgtttcgca tattcgcgcc gggtgttcgg cgttggcgtc caggttggtg
     8161 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     8221 cgataccaat aggaaagact tccactgtat tactgtctgg cggatagtaa taaagacgca
     8281 tttcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     8341 gaatggtgag ttgtgagcca ggcttcggca aaaaaacatc agcgcccgga ttcgcttcca
     8401 gcatattact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgagtattgt
     8461 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     8521 gataagttac cgccaggcta ttttggctgg caaaaagcag agcaaatgag caaagaatat
     8581 ttacacgacg catcatgtcc ctttcctaag tcgcgaatac tatccgataa gtatagcttt
     8641 tatcagactt ttcgttttta gctgttcaaa tcagaagtcg tattccccgg tagaacaata
     8701 ttactggcag caagttcgcc catgttgttg tatatcgcac aagcagcttc gatgatggac
     8761 atcgccagag ctgcaccact cccctcacct aaacgcatat ccatattgag ataaggctcc
     8821 agccccaaat gcgagagcgc tatacgcgcg cctttttctg ccgacaagtg agaaggaatg
     8881 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcagcagc ataagaaaga
     8941 aatccatcca gcaacacggg taaaccgcag gaagcagcac ctaacatcac tccagctatt
     9001 ccgaccaaat caaatccacc cacttttgcc aggacatcaa caccatcctg aggatttggt
     9061 tgattcaacg taatcgcccg acgcacaaca tcaattttat tagccagttt atctgtcggc
     9121 aggtttgcgc caatcccaac cacttcttca ggatcccggc cagtgattgt gctgactatt
     9181 gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta caccaaacag cgtgacaccg
     9241 tttttcgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc agcctgacgg
     9301 cgactcattg ccggagctga agcaatattg ccgctacctc gtgcgacacg catgttgata
     9361 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtggac gttagcgccc
     9421 gcttgtgctg ccagcacaca cacgccggtt gttccacggg tcatattttc agcctgtatg
     9481 gctgtcactt cttttggaga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     9541 ataaccagta ccgctttttt gcccacatgc ggtatgccat tcacccccgg cattcctgcc
     9601 agttgtatgg caagcgcctc cagctttcct aggctaccaa caggtttgaa taacccgtca
     9661 acatgccgtt gtgcacgcga catagcggca gaattgatgg ctgggatcgt attcagtaaa
     9721 tcggcaatta tttgcatgtc acgtttcttt atagcagtgc cagcaggaag accagttcac
     9781 caagctctat ggctgctccc agcgtatcgc ccgtttgccc gcccagcgta cgtttgagaa
     9841 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     9901 ccagcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     9961 catcaatttt gccaatgaac acattgccaa gcccttcttc ccgcgcataa cgatgacgat
    10021 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
    10081 tcggttcgcc acgcagagcc aactcgctta acaccagaat ctttgccagt accacaaaaa
    10141 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
    10201 cgcgactacg tgcagaaaat acgccatcac aggtatcagc gaggccgtcc agatggaatt
    10261 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt gcgccacacc
    10321 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaatcaaag
    10381 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
    10441 gtacaggcag acgcgtaata aatgagagca tcgcccaaaa taatttgctc atttgatttt
    10501 tactccaata cccgaaacca ccagccatac ttcatttgcc gcagcggcca actgctgatt
    10561 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
    10621 tcccacttcg ttagtcacta atacaacctt tgcggggcaa cgttggcagg cagcaatcaa
    10681 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
    10741 tttatcgccg ccataatcaa acaacagatt agtcaccatt gtggtaacgc attccagcaa
    10801 cacagcctca ttcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
    10861 cactgtgcgc cagtgctccg ggcggctttg ccgatgatgt tctatccgtg cagccatctc
    10921 atcatcaagg atttgcgagg tagcgatata cagaacctgt gaa
//
LOCUS       DAOFMX010000118        10926 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.118, whole genome shotgun sequence.
ACCESSION   DAOFMX010000118 DAOFMX010000000
VERSION     DAOFMX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.118"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            154..504
                     /locus_tag="QBA20_003692"
     CDS             154..504
                     /locus_tag="QBA20_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDQ6294076.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="QBA20_003693"
     CDS             557..952
                     /locus_tag="QBA20_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDQ6294077.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="QBA20_003694"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="QBA20_003694"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDQ6294078.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="QBA20_003695"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="QBA20_003695"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDQ6294079.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="QBA20_003696"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="QBA20_003696"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDQ6294080.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLSAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="QBA20_003697"
     CDS             complement(5323..6423)
                     /locus_tag="QBA20_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDQ6294081.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="QBA20_003698"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="QBA20_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDQ6294082.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="QBA20_003699"
     CDS             complement(7571..8137)
                     /locus_tag="QBA20_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDQ6294083.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="QBA20_003700"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="QBA20_003700"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDQ6294084.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="QBA20_003701"
     CDS             10263..10751
                     /locus_tag="QBA20_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HDQ6294085.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2670 a         2790 c         2603 g         2863 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctattcg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcgctc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 tatagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgagtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cgtggaatta
    10921 accgcc
//
LOCUS       DAOFMX010000119        10579 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.119, whole genome shotgun sequence.
ACCESSION   DAOFMX010000119 DAOFMX010000000
VERSION     DAOFMX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.119"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="QBA20_003702"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="QBA20_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDQ6294086.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="QBA20_003703"
     CDS             complement(2005..2928)
                     /locus_tag="QBA20_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6294087.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="QBA20_003704"
     CDS             3145..4488
                     /locus_tag="QBA20_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDQ6294088.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="QBA20_003705"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="QBA20_003705"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDQ6294089.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="QBA20_003706"
     CDS             6508..6732
                     /locus_tag="QBA20_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDQ6294090.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="QBA20_003707"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="QBA20_003707"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDQ6294091.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /locus_tag="QBA20_003708"
     CDS             complement(7326..8306)
                     /locus_tag="QBA20_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294092.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="QBA20_003709"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="QBA20_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDQ6294093.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="QBA20_003710"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="QBA20_003710"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDQ6294094.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..>10579
                     /locus_tag="QBA20_003711"
     CDS             10315..>10579
                     /locus_tag="QBA20_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HDQ6294095.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQK"
BASE COUNT         2719 a         2477 c         2660 g         2723 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaata attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 taatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggcgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcagg
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatattct ggttttttaa acaattactc tgatttaaaa
    10441 gaaacaacct cagcaacagg taaacctgtt ttacgttggg tagacccgag ttttgatcaa
    10501 agcaaatatg acagcatcgt ctggaaccca atcacttatt atccggtacc aaaaccttct
    10561 acccaggtag ggcagaaag
//
LOCUS       DAOFMX010000120        10428 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.120, whole genome shotgun sequence.
ACCESSION   DAOFMX010000120 DAOFMX010000000
VERSION     DAOFMX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.120"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..568
                     /locus_tag="QBA20_003712"
     CDS             <1..568
                     /locus_tag="QBA20_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDQ6294096.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            526..1203
                     /gene="bglJ"
                     /locus_tag="QBA20_003713"
     CDS             526..1203
                     /gene="bglJ"
                     /locus_tag="QBA20_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDQ6294097.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1241..2029)
                     /gene="fhuF"
                     /locus_tag="QBA20_003714"
     CDS             complement(1241..2029)
                     /gene="fhuF"
                     /locus_tag="QBA20_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDQ6294098.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2170..2406
                     /locus_tag="QBA20_003715"
     CDS             2170..2406
                     /locus_tag="QBA20_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDQ6294099.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2445..2531)
                     /locus_tag="QBA20_003716"
     tRNA            complement(2445..2531)
                     /locus_tag="QBA20_003716"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2495..2497),aa:Leu,seq:cag)
     gene            complement(2808..3839)
                     /gene="rsmC"
                     /locus_tag="QBA20_003717"
     CDS             complement(2808..3839)
                     /gene="rsmC"
                     /locus_tag="QBA20_003717"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDQ6294100.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            3942..4355
                     /gene="holD"
                     /locus_tag="QBA20_003718"
     CDS             3942..4355
                     /gene="holD"
                     /locus_tag="QBA20_003718"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDQ6294101.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4324..4770
                     /gene="rimI"
                     /locus_tag="QBA20_003719"
     CDS             4324..4770
                     /gene="rimI"
                     /locus_tag="QBA20_003719"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDQ6294102.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            4785..5462
                     /gene="yjjG"
                     /locus_tag="QBA20_003720"
     CDS             4785..5462
                     /gene="yjjG"
                     /locus_tag="QBA20_003720"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDQ6294103.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5553..7142
                     /gene="prfC"
                     /locus_tag="QBA20_003721"
     CDS             5553..7142
                     /gene="prfC"
                     /locus_tag="QBA20_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDQ6294104.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7535..8140
                     /gene="osmY"
                     /locus_tag="QBA20_003722"
     CDS             7535..8140
                     /gene="osmY"
                     /locus_tag="QBA20_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDQ6294105.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8257..8418
                     /locus_tag="QBA20_003723"
     CDS             8257..8418
                     /locus_tag="QBA20_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDQ6294106.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8540..9613
                     /locus_tag="QBA20_003724"
     CDS             8540..9613
                     /locus_tag="QBA20_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDQ6294107.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9610..10392
                     /locus_tag="QBA20_003725"
     CDS             9610..10392
                     /locus_tag="QBA20_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDQ6294108.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
BASE COUNT         2548 a         2590 c         2785 g         2505 t
ORIGIN      
        1 attagtcatt gctgatttag ccggtgaaag tgaagatcca cgttctgttt gtgaacatta
       61 ttattcttta atctcacaat atcgggaaat tcactgggtt ttcatggtgt cacgctcctg
      121 gtattcccag gctgtagaac tgctcatgtg ccctacggcg acgttattgt ctgatgttga
      181 acccattgag aatctggtca agaccgtacg ttccggcaat acgcacgcag agcgtatcag
      241 cgccatgctg acctctccgg caatgactga aactcatgat tttagctatc gctccgtcat
      301 tctcactctt tcagagcgca aggtactgcg gctattaggt aaaggatggg gcatcaacca
      361 gatagcttca ttgcttaaga aaagtaataa aactatcagc gcccaaaaaa acagtgcgat
      421 gcgtcgactg gcaattcaca gcaacgctga aatgtatgca tggataaata gcgcgcaggg
      481 tgcaagagaa cttaacttgc cttctgttta tggagatgcc gcagaatgga acacagccga
      541 attaagaaga gaaatgtcgc actcatagaa aaatgcgtca tgagtagtat cggtattgag
      601 agtttattca gaaagtttgc gggcaaccct tataagctcc atacctatac cagtcaggag
      661 tcatttcagg atgccatgtc gcggatctcg tttgcggcgg tcattttttc tttttctgcc
      721 atgagaagtg agcgcagaga gggattatct tgcctgactg agctggcgat taagtttccg
      781 cgtacccggc gtttagttat tgtggatgat gatattgaag ctcggctgat tggttcattg
      841 tcgccatcac cgctggacgg tgtattaagt aaagcgtcaa cgctggagat ttttcatcag
      901 gaactctttt tgtcattaaa tggtgtacgt caggcgaccg accgactgaa caatcagtgg
      961 tacattaacc aaagccggac gttaagcccg acggagagag aaatattgcg ctttatgtcg
     1021 cgtggctact caatgacaca aattgccgag cagcttaaac gtaatatcaa aacgatccga
     1081 gcgcataaat ttaatgtgat gtcgaaactg ggcgtcagtt ctgatgcagg gttgttggag
     1141 gccgcagata ttctgttatg tatgcggcat tgcgaagcaa gtaatgtgtt gcatccctat
     1201 taatccgcat gatgccgggt ttacttcccc ggcagtgctt tcatttcagc gtacaatagc
     1261 cacattgctg cacatccggt aagcgataac gctggcagca agtgcggcgc accagcaggc
     1321 cgtcgcgcag taccacggta cgccagagtg gattatcttc accgttcgtg agcgttttct
     1381 caaaaaagag ggcatggcgc agcgattcaa cagtagcctc gccgagcagt tgcttcatct
     1441 cagtgagata ccagttgatc aaataaccgg tattactcca gataagtttg ccgttgatct
     1501 ctccggtcgc ttctagtgct tgcacaaccg gaaccagcgc ctggctgatt aacgtttcca
     1561 ttcgctgctg cggcgaatgt ggtgttgcgt ttttatcttc acacacatcg acccagaaac
     1621 aggcgacgcg tccggtttcg tgaaactcag catggaaatg ttccggcgac acatctaatg
     1681 ctttttcctg cgtcagtagc gccagcatta atggtggcac catcaggccg atataccatt
     1741 gtgcccatag tgagatcagc ggtttgttct cgcggatcat cgttggttgg ttgcgataga
     1801 tatgatcgga atagaccgcc agtagagaac ttagcgcatt cggtgatgac cattgcgcca
     1861 gcgtcatggc gttaagcgga gcgggttcat ccaggcggat aaactccagc aaatgttcac
     1921 gatgtttggc gatcatcgcc cgtacggctt gtgcaagccc agcatcctgc ggctggagat
     1981 gcgttcgcca gatgacatct tcatagagcg gtgcggaacg ataggccata atcgtgatag
     2041 taatctaaat gataatgatt gctaatccta gcgataggtt tactcgatag caagggattt
     2101 ctctggcttg caaatgataa aaattatcat atgatattgg ttttcattat caatgaaaga
     2161 gatgaaacca tgttgcaacg tacgctgggc agtggctggg gagtgttgct gccgggattg
     2221 ctgattgcag ggctgatgta tgcggattta tcgccggatc agtggcggat tgtcattctg
     2281 atgggattag tattgacgcc ggtgatgctg tatcacaaac aattgcggca ttacattttg
     2341 ctaccatcgt gcctggcgct tattgctggc atcatgctga tgataatgaa tttgaatcag
     2401 ggatgacaaa acagggaaga aacaagaaag gaagtaaaga taattggtgc gagggggggg
     2461 acttgaaccc ccacgtccgt aaggacacta acacctgaag ctagcgcgtc taccaattcc
     2521 gccaccttcg cataccatca attcttaaaa agaattgcta ccacggaggc gcattctagt
     2581 ggttttcagc ttttcgtcaa tagttaatta tcgcctgatg tgtaattgct ggaaaaatgt
     2641 ccatcaggaa actagcgtgc aggtttggta ggcatgcggg ggcagatgcc agatgcgacg
     2701 ctggcgcgtc ttatctggcc tacgaagggc taacgtgcag gttttgtagg tcggataagg
     2761 cgttcacgcc gcatccgaca cggtatgcgg cgacagatat cagatactca acctttcttc
     2821 gcctggcggg tcataatggc gcgatatacc ttgaagcgcc cggtttgcgc gataacttca
     2881 tggaagccaa atgtctcatc cagcacgtcc gggtaaggca ggaaggcgtt cgctacaatt
     2941 cgcagctcgc cgccgctatt aagatgacgc accgcgccac gaatcagcgt ttgcgccgca
     3001 tccaggctgg tttgcatccc atcgtggaac ggcgggttgg agatgatcat atcaaaacaa
     3061 cctttcacct cggaaaagac gttgctggca aagacttcac cttcaacacc gttggctgca
     3121 agtgttgcgc ggctggcttc taccgccggg gcagagacat cgcacaaggt gagacgaatt
     3181 ttcggcgaat ggcgcgcaaa ggcaactgaa agtacacccg cgccacagcc gacatccagc
     3241 actttacctt tcgtgtgcgg agttaacgtc gagagcagca actggctacc gacatccaga
     3301 ccgtcgcggc taaacacgcc aggcagcgtt ttgaccgtca ggccatcgac gctgtattcg
     3361 ccccagaatt tatccgcatc aaatactggc tgtttttcca gacgaccaaa atagaggcca
     3421 cagcgacgag cgctgtcgac tttattcaac ggcgcataat ctgccagcat ctgctcagcg
     3481 ctgcgcacgc cgctgcggtt ctcgccaacg acaaaaatat ctgtccccac tggcagcaga
     3541 gaaagtaaat tcatcaactg gaactgggct tccggtttgt tcttcggcca gtagtaaatc
     3601 agcgtatcgc aatctgcgac atcattcgcc gtggcgacca gactaaaacg ggcgttatcc
     3661 cccatctggc agcttaacac ctgccaatgg tggaattgct gggtatgagc acggctggcc
     3721 gcggtatcta aacgcgcggg caggtcatcc tgtaagtctc cggcaaacag aatacggctt
     3781 tgttcgaaat catcactgtg acgcagcaag acttcacttg ccggggtaaa tgcagacatg
     3841 gaatgctcct caattgatac tggcggcgat tatagccata tgttggcgcg gtatcgacga
     3901 atttgctata tttgcgcccc tgacaacagg agcgattcgc tatgacatcc cgacgagact
     3961 ggcagttaca gcaactgggc attacccagt ggtcgctgcg tcgccctggc gcgttgcagg
     4021 gggagattgc cattgcgagc ccggcacacg tccgtctggt gatggtggca aacgatcttc
     4081 ccgccctgac tgatccttta gtgagcgatg ttctgcgcgc attaaccgtc agccctgatc
     4141 aagtgctgca actgacgcca gaaaaaatcg cgatgctgcc gcaaggcagt cgctgcaaca
     4201 gctggcggtt gggtactgac gaaccgctat cattggaagg cgctcaggtg gcatcaccgg
     4261 cgctcaccga attacgggca aacccaacgg cacgcgccgc gttatggcaa caaatttgca
     4321 catatgaaca cgatttcttc cctcgaaacg actgatttac cagcggctta ccacattgaa
     4381 caacgcgccc acgcctttcc gtggagtgaa aaaacctttg ccagcaacca gggcgagcgt
     4441 tatctcaact ttcagttaac gcaaaacggc aaaatggcgg cgtttgcgat tacgcaagtg
     4501 gtgctggatg aagctacatt gttcaatatt gcggtcgatc ccgactatca gcgtcagggg
     4561 ctgggtaggg cgctgctgga acatctgatc gacgaactgg aaaaacgcgg cgtggcgaca
     4621 ctatggctgg aagtccgtgc ctcaaatgct gccgccattg ccctgtacga aagtttaggc
     4681 tttaacgagg cgacgattcg ccgcaattac taccccacca cggacggtcg cgaagacgcc
     4741 atcatcatgg cgttgccaat cagtatgtaa gacaaggtgg aataatgaag tgggactgga
     4801 ttttctttga tgccgatgaa acgctgttta cctttgactc attcaccggc ctgcagcgga
     4861 tgtttcttga ttacagcgtc acctttaccg ctgaagattt tcaggactat caggccgtta
     4921 acaagccact gtgggtggat tatcaaaacg gcgcgatcac ttcattacag cttcagcacg
     4981 ggcgtttcga gagctgggcc gaacggctga aagttgaagc aggcttgctt aacgatgcct
     5041 ttattaatgc gatggcggaa atctgtacgc cgttgccggg cgcggtttct ctgcttaacg
     5101 ccattcgtag caacgctaaa atcggcatca tcaccaacgg ttttagcgcc ttgcagcaag
     5161 tgcgtctgga acggacgggc ctgcgtgatt acttcgattt gctggtgatt tccgaagaag
     5221 ttggcgttgc caaaccgaat aagaaaattt tcgattatgc gctggaacag gcgggcaatc
     5281 ctgaccgttc acgcgtgctg atggttggcg acactgccga gtccgatatt ctcggtggca
     5341 tcaacgccgg gcttgcgacc tgctggctga atgcgcacaa tcgcgagcaa ccagaaggca
     5401 tcgcgcccac ctggaccgtt tcttcgttgc acgaactgga gcagctcctg tgtaaacact
     5461 gattgcctcc cccccgttga tgggtaaaat agccgcaatt tttcgttttc aacaagcgcg
     5521 gcgcgatgcc gcttactcaa gaagaaagaa ttatgacgtt gtctccttat ttgaaagagg
     5581 tggcgaagcg ccgcactttt gccattattt ctcacccgga cgccggtaaa actaccatca
     5641 ccgagaaggt gctgctgttc ggacaggcca ttcagaccgc cggtacagta aaaggccgtg
     5701 gttccaacca gcacgctaag tcggactgga tggagatgga aaagcagcgt gggatctcca
     5761 ttactacgtc tgtgatgcag tttccgtatc acgattgcct ggttaacctg ctcgacaccc
     5821 cggggcacga agacttctcg gaagatacct atcgtaccct gacggcggtg gactgctgcc
     5881 tgatggttat cgacgccgca aaaggtgttg aagatcgtac ccgtaagctg atggaagtta
     5941 cccgtctgcg cgacacgccg atcctcacct ttatgaacaa acttgaccgt gatatccgcg
     6001 acccgatgga actgctcgat gaagttgaga acgagctgaa aattggctgc gcaccgatca
     6061 cttggccaat tggctgcggc aagctgttta aaggcgttta ccacctttat aaagatgaaa
     6121 cctatctcta tcagagcggt aaagggcaca ccattcagga agtccgcatt gttaaagggc
     6181 tgaataaccc ggatctcgat gctgcggttg gtgaagatct ggcacagcag ctgcgtgacg
     6241 aactggaact ggttaaaggc gcgtctaacg agttcgacaa agagctgttc cttgcgggcg
     6301 aaatcactcc ggtattcttc ggtactgcgc tgggtaactt cggcgttgat catatgctga
     6361 atggcctggt ggagtgggcg cctgcgccga tgccgcgtca gaccgatacc cgtaccgtag
     6421 aagcgagcga agataaattt accggcttcg tatttaaaat tcaggccaac atggatccga
     6481 aacaccgcga ccgcgtggcg tttatgcgtg tggtgtccgg taaatatgaa aaaggcatga
     6541 aactgcgcca ggtgcgcact gcgaaagatg tggtgatctc cgacgcgctg acctttatgg
     6601 cgggtgaccg ttcgcacgtt gaagaagcgt atccgggcga tatcctcggc ctgcacaacc
     6661 acggcaccat tcagatcggc gacaccttta cccagggtga gatgatgaag ttcaccggta
     6721 ttccgaactt cgcaccagaa ctgttccgtc gtatccgcct gaaagatccg ctgaagcaaa
     6781 aacagctgct caaagggctg gtacagcttt ccgaagaggg cgcggtgcag gtgttccgtc
     6841 caatctccaa caacgatcta atcgttggtg cggttggtgt gctgcagttt gatgtggtgg
     6901 tagcgcgcct gaagagcgaa tacaacgttg aagcagtgta tgagtcagtc aacgttgcca
     6961 ctgcccgctg ggtagaatgt gcagacgcga agaaattcga agagttcaag cgtaagaacg
     7021 aaagccaact ggcgcttgat ggcggcgata acctcgctta catcgctacc agcatggtca
     7081 acctgcgcct ggcacaggaa cgttatccgg acgttcagtt ccaccagacc cgcgagcatt
     7141 aattcttgcc tccagggcgc ggtagccgct gcgccctgtc aatttccctt ccttattagc
     7201 cgcttacgga atgttcttaa aacattcact tttgcttatg ttttcgctga tatcccgagc
     7261 ggtttcaaaa ttgtgatcta tatttaacaa agtgatgaca tttctgatgg cgttaaatac
     7321 cgttcaatgc gtagatatca gtatctaaag ccgtcgattg tcattctacc gatattaata
     7381 actgattcag aggctgtaat ggtcgttatt catcacccat cgcttttgtg atggcgacca
     7441 ttgacttctg tagagggtga agtctctccc tattcagcaa tgcaacctcg tgttgctagg
     7501 ctcaaattac gagcaaacat acaggaataa atcgatgact atgacaagac tgaagatttc
     7561 gaaaactctg ctggctgtaa tgttgacctc tgccgtcgcg accggctctg cctacgcgga
     7621 aaacaacgcg cagactacca atgaaagcgc agggcaaaaa gtcgatagct ctatgaataa
     7681 agtcggtaat ttcatggatg acagcgccat caccgcgaaa gtgaaggcgg cgctggtgga
     7741 tcatgacaac atcaagagca ccgatatctc tgtcaaaacc gatcaaaaag tcgtgaccct
     7801 gagcggtttc gttgaaagtc aggcccaggc tgaagaggca gtgaaagtgg cgaaaggcgt
     7861 tgaaggcgtg acctctgtca gcgacaaact gcacgttcgc gacgctaaag aaggctcggt
     7921 gaagggctac gcgggtgaca ccgccaccac cagtgaaatc aaagccaaac tgctggcgga
     7981 cgatatcgtc ccttcacgta aagtgaaagt tgaaaccacc gatggcgtgg ttcagctctc
     8041 cggtaccgta gattctcagg cacaaagtga ccgtgctgaa agtatcgcca aagcggtaga
     8101 tggtgtgaaa agcgttaaaa atgatctgaa aactaagtaa ttcgtcctcc cgaactttgt
     8161 cgggaggcgc gatgtgcacc acactaaaaa tatcgcattg agtgattttc acgctcatat
     8221 caagcggtga cattaactat ggtaaaggag acgcttatgt ttcgttgggg catcatattt
     8281 ctggttatcg cgttaatcgc cgccgcactt gggtttggtg gtctggccgg taccgctgca
     8341 ggcgcagcta aaattgtctt tgtcgtcggg attattctgt tcctagtgag tttgttcatg
     8401 ggccgaaaac gaccctagat ttcagaccat taagaaaatt ttatccaaag ccagtccagc
     8461 ggactggctt ttgcggtttt agcgaataat aaattgcgtt actttgtctt tctttcacaa
     8521 caaaaacagg aaggcagaag tggggcagcg aatacctgta acgcttggta atattgcgcc
     8581 gttgtcgcta aggccgttcc agcctggacg aatagctctg gtgtgcgaag gcggcggaca
     8641 gcgtggaatt ttcacggctg gcgtgctgga tgagtttatg cgcgcgcagt ttaatccttt
     8701 cgatctttat ctcggcacat ctgccggggc gcagaacctc tcggcgttta tctgcaatca
     8761 gcccggttac gcgcgcaaag tcatcatgcg ctataccaca aaacgcgaat ttttcgatcc
     8821 attgcgcttt gtccgtggag gaaatcttat cgatctcgac tggctggtgg aggccaccgc
     8881 aagccagatg ccgttgcaaa tggataccgc cgcgcggttg tttgacagcg gcaaatcgtt
     8941 ttatatgtgc gcctgtcgtc aggatgacta cgcgccgaat tactttttac caaccaaaca
     9001 aaactggctg gatgtgattc gcgcctccag tgcgatacct ggcttttatc gtagcggagt
     9061 gtcgctggaa ggcattaact acctggatgg cgggatcagt gatgcgattc cggttaaaga
     9121 ggcggcaagg cagggcgcta aaacgttggt cgtcatccgc actgtgccgt cacaaatgta
     9181 ctacacgccg cagtggttca aacgcatgga acgctggctg ggtgacagta gcctgcagcc
     9241 gctggtcaat ctggtgcagc atcatgaaac cagctatcgt gacattcagc aatttattga
     9301 gaaaccaccg ggcaagctgc ggatatccga aatttatccg ccgaagccat tacatagtat
     9361 cgcgcttggc agtcggattc cggcgctgcg tgaagactat aaacttgggc gtttatgcgg
     9421 tcgttatttc ctcgccacgg ttggcaagct attaactgaa aaagcgccgc ttacccgcca
     9481 tctggtgcca gtggtgacgc cggaatcgat tgtcattccg cctgcgccag tcgccaacga
     9541 tacgctggtt gccgaagtga gcgacgctcc gcaggcgaac gacccgacat ttaacaatga
     9601 ggatctggct tgatttgccg ttttatcgac actcactgcc attttgattt cccgcctttt
     9661 attggcgatg aagaggccag cctgcaacgc gcggcacaag cgggcgtagg caagatcatt
     9721 gttccggcga cagaggcgga aaattttgcc cgcgtgcagg cgttagcgga aaattatcaa
     9781 ccgctgtatg ccgcattggg cttgcatcct ggtatgttgg aaaaacatag cgatgtgtct
     9841 cttgagcagc tacagcaggc gctggaaagg cgtccggcga aggtggtggc ggtgggggag
     9901 atcggtctgg atctcttcgg cgacgatccg caatttgaga ggcagcagtg gttactcgac
     9961 gaacaactga aactggcgaa acgctacgat ctgccggtga tcctgcattc acggcgcacg
    10021 cacgacaaac tggcgatgca tcttaaacac cacgatttgc cgcgtgctgg tgttgtccac
    10081 ggattttctg gcagccagca acaggctgaa cggtttgtac agctgggcta caaaatcggc
    10141 gtaggcggta ctatcaccta tccacgcgcc agtaaaaccc gcgatgtcat cgcaaaatta
    10201 ccgctggcat cgttgttgct ggaaaccgat gcgccggata tgccgctcaa cggttttcag
    10261 gggcagccta accgcccgga acaggctgcc cgtgtgtttg ccgtgctttg cgagttacgc
    10321 ccggaacccg cggatgagat tgccgaagtg ttgcttaata acacgtatgc ggtgtttaac
    10381 gttcgtgggt agtttgccct tcgtaggcag catctggcag tcggcgca
//
LOCUS       DAOFMX010000121        10219 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.121, whole genome shotgun sequence.
ACCESSION   DAOFMX010000121 DAOFMX010000000
VERSION     DAOFMX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.121"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..65)
                     /locus_tag="QBA20_003726"
     CDS             complement(<1..65)
                     /locus_tag="QBA20_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6294109.1"
                     /translation="MMMELQHQRLMALAGQLQLESL"
     gene            complement(62..418)
                     /locus_tag="QBA20_003727"
                     /pseudo
     CDS             complement(62..418)
                     /locus_tag="QBA20_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            498..1691
                     /locus_tag="QBA20_003728"
     CDS             498..1691
                     /locus_tag="QBA20_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HDQ6294110.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1717..2394)
                     /gene="istA"
                     /locus_tag="QBA20_003729"
                     /pseudo
     CDS             complement(1717..2394)
                     /gene="istA"
                     /locus_tag="QBA20_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
     gene            complement(2481..2842)
                     /locus_tag="QBA20_003730"
                     /pseudo
     CDS             complement(2481..2842)
                     /locus_tag="QBA20_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3181..3735)
                     /locus_tag="QBA20_003731"
                     /pseudo
     CDS             complement(3181..3735)
                     /locus_tag="QBA20_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            3878..4000
                     /locus_tag="QBA20_003732"
                     /pseudo
     CDS             3878..4000
                     /locus_tag="QBA20_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(4262..4828)
                     /gene="ipgB2"
                     /locus_tag="QBA20_003733"
     CDS             complement(4262..4828)
                     /gene="ipgB2"
                     /locus_tag="QBA20_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HDQ6294111.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(5110..5424)
                     /locus_tag="QBA20_003734"
     CDS             complement(5110..5424)
                     /locus_tag="QBA20_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294112.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(5502..5612)
                     /locus_tag="QBA20_003735"
                     /pseudo
     CDS             complement(5502..5612)
                     /locus_tag="QBA20_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5936..6613)
                     /gene="ospD1"
                     /locus_tag="QBA20_003736"
     CDS             complement(5936..6613)
                     /gene="ospD1"
                     /locus_tag="QBA20_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HDQ6294113.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(6984..7121)
                     /locus_tag="QBA20_003737"
                     /pseudo
     CDS             complement(6984..7121)
                     /locus_tag="QBA20_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(7203..7501)
                     /locus_tag="QBA20_003738"
                     /pseudo
     CDS             complement(7203..7501)
                     /locus_tag="QBA20_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8090..8249)
                     /locus_tag="QBA20_003739"
                     /pseudo
     CDS             complement(8090..8249)
                     /locus_tag="QBA20_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8253..9482)
                     /locus_tag="QBA20_003740"
                     /pseudo
     CDS             complement(8253..9482)
                     /locus_tag="QBA20_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(9502..9849)
                     /gene="tnpB"
                     /locus_tag="QBA20_003741"
     CDS             complement(9502..9849)
                     /gene="tnpB"
                     /locus_tag="QBA20_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDQ6294114.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(9849..>10219)
                     /locus_tag="QBA20_003742"
     CDS             complement(9849..>10219)
                     /locus_tag="QBA20_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6294115.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
BASE COUNT         2642 a         2386 c         2311 g         2880 t
ORIGIN      
        1 aggctttcca gttgcaactg cccggcgagc gccatcagtc gttgatgttg cagttccatc
       61 atcacgccac tcctctgcag aatgagtcgt agatggagag tggatgatgc tggggggggt
      121 tgtcgaagct caccagattt tcaccaggat gcacgtcata ctcttttttc tccggaggca
      181 gtgccagcat ggactgctgc tcttcgagcc agcgatcaca gggacgggcc tggattgttt
      241 catgctttcg ttggttagca acatcgtgca gccagcgcag accgtggcgg ttggctgttt
      301 caacatcgac agtgctcccc atcgggcgca ggcgagtcat tagtgggatg taaaaactgt
      361 tacgggtgta ctgcaccatc cgttccacct tacctttagt ctgtgccctg aagggctgag
      421 gaatccccag aaaacgagac aaacatagca ttatgtgatc tactgatttg tgccaacaat
      481 tcaatgagat cactgctatg cctgttcata aagtatgtca gaatttcttc cgggatgcct
      541 tagcaccatt tcatcagtac cgacaaaatg ccctgatgga tgccacaatg gcgttaatca
      601 acggagcatc tctgacgcag accagtatcg gacgtttttt gccgggtaat gcacaagtta
      661 agaataagat caaacgtatt gatcgcctca tgggtaatga ggcgctccat cgcgatattc
      721 caatgatttt caggaatatc acttcaatgc tgacacggca gttatcttta tgtgtaatag
      781 ccgtagactg gagtggctat ccctcccagg agcatcatgt tcttcgtgcc agccttctgt
      841 gtgacgggcg ttccattccg ttattgagta aggttgttcc ttctgaaaaa caaaataatc
      901 cattgataca acatgatttc cttgattccc ttgctcaatc cctgcccccc gatgccaggg
      961 ttattattgt cacggatgct ggatttcaga gcgcatggtt tcaccatatt acttctctgg
     1021 gctgggattt tattggtcgg atcagaaata acgttcagta ttgcctcgat aacgcgcctg
     1081 aaagatggtt aaaagtgtcg gatagccctg aatgtaaaac accggaatat atgggggctg
     1141 gaaggctggt taaagaaagg aaaaaaagca tccgggggca tttttacacc tataagaaat
     1201 cggcaaaagg gcgaaaaaag aaacgttcga aaggtcagag tggtttaaac aaaacagaca
     1261 aagaacagag caaatcagca aaagaagcct ggttgatctt cagcagtaca aatgacttca
     1321 gagcgcgaga aatcatcaag ttatacagtc ggcgaatgca gatagagcaa aactttcgtg
     1381 atgaaaagaa cgggcgcttc ggatttggtc ttcgggccag caaaagtcgt tcaacaggaa
     1441 gaattctggt tctgagtctg ctggcaacgt tgagcactat cgtaatgtgg ttactcggtt
     1501 atcacgctga aaataaaggg ttacacctga aatatcaggc taacagcatt aaatcccggc
     1561 gggttatctc atatctgacg ttagcgaaga atgttcttcg acactcgcca cttattttaa
     1621 gacgaacagt actgagcaca gttctaaacc atctgtccag aacctaccga aatatggtgc
     1681 tggtttatta gcagtaattt tgtggggatc cctcagcctg aaggggcgac acagtcgggg
     1741 agagaagccc atctccttgc cgaactgcca cagcgaagga tggaaccggt gctgaccggt
     1801 ctgatatgcg tcacgttgca gaaccacagt tttcatattg tcatacaaca cttcgcgcgg
     1861 cacaccacca aagaagcgga acgcattacg atggcaggtc tccagcgtgt cataacgcat
     1921 attgtcagtg aattcgatgt acaacattcg gctgtatccg ggaacagcaa cgaacacgtg
     1981 aagcggtgag cgaccattac gcatagtgcc ccagtcaacc tgcatctgtc gtccgggttc
     2041 agtttcgaac cgaacggcag gctcctgctc ctgaggaacc gagagagaac gaatgaatgc
     2101 cctgagaatg gtcattccgc cacgatatcc ctggtctctg atctcgcgag cgattaccgt
     2161 tgccgggatt ttgtaaggat gagcatcggc gatgcgttga cgaatataat cccggtattc
     2221 atccaggagt gaagcaacag caggtcgcgg cgtatatttt ggcggctcag attttgcctg
     2281 caaataacgt ttaacggtat tgcgggagag ccccagttct ctggcaatcg cccggctact
     2341 cattccctgc ttgtgcagga ttttaatttc cataactgtc tcaaaagtga ccataagctc
     2401 tcctgaatca ggagagcaga ttaccccctg gatctgattt caggcgttgg gtgtggatca
     2461 ctattgcacc gttcgtgaca gtgttgttgc gccttgctgc caacgaagct ccactgccca
     2521 tcaagttcac agataagggc aacatcagcg tgagcgactg gcgcagatgt tatccgccgg
     2581 ggcgggagct ttttaatgtc cgtatgacgg tattgatgcc gattttcagc gtacggccag
     2641 tatcgcgaac tccggcaccg ttgaaagcca tttcagtgat ttggttttta actcccggtt
     2701 tacgggcctc gtaacagtaa gtgagctgaa acacccggtg acagtcacgg caacggaatc
     2761 gatcatgtcc ttttgggttc tgcccgtgac ggtaaacctg aacggactgg caacgggggc
     2821 aatgaaagtt aactgtgacc atgaaagaac ctaaaagcgg gtattataac ccctaattca
     2881 actaattgga ggcattgtca tttttaatct atggatacac ttaaaaatcc tctcacctta
     2941 cgttaaaaac cttctcatgt ttagagtttt ttattatgtt tatcataatg atatgacgat
     3001 ctgagttttt aaaatcaaga agaaaagata agttttttaa atgcacagtg cctgtggata
     3061 actttgcgta aatccggagg acagatcact ctgaagcaag acaacagcat ctgaaaacaa
     3121 tatgctcacg tttcaaccac agggcgtaag tgatcctacc cacgtaatat ggacacaggc
     3181 ctaagcgagg ttctggtttt caaattgttc cggactgagg ccgccacacc aactgtgccg
     3241 tcgccagcga ttgtaatcac attcgatata attaaacact gttgcccgca ttatttcccg
     3301 gctgataaag tgttctccgt ggagacattc cactttcagc gaatgaaaga agctttccac
     3361 gcaggcatta tcgtagcagc agccctttgc gctcatactg ccacgcagat tatgccactt
     3421 cagcagcgcc tgataatctg ctgaacagta ctgacttcct cggtccgagt gaacgataac
     3481 gttccggggt ctcttacgcc gccacagcgc catctgcagg gcatcgcagg ccagttgcgc
     3541 cgtcaggcgt ggtgacatgg accagccaat aacggcacgt gaccacaggt caatgaccac
     3601 agccagatac agccagcctt catctgtacg taagtacgtg atgtctcccg cccacttctg
     3661 gttcggacca ctggcgtaaa aatcctgctc cagcagattt tctgacacag gcaggccatg
     3721 tgcgcggtaa ctgaccgtat tgctgggtaa gatcaatttg ctcactggta tttactacgt
     3781 gcatcgaatg agtgatcaag agccgtatga cgcgagagtg tcacgtacgg ttccgtaagc
     3841 agccggagag tgaaactctc tccggctgac tcgactcgca ggttccgaca gcggtgacga
     3901 acatgcagcg gtgttgttct cgctgatcgg cacatgccgt ctgaacaatg tggagccaga
     3961 aaaatggctg cgttacgtta ttgaacatat ccaggactga ccggcaaacc gggtacgcga
     4021 tctgttgcct tggaaagttg atctgacttc tcagtaaata tcaatatgat tctgacgagc
     4081 cgcttacctt tgagcagaga aaggggagag ggagcccaca tccatgagct aggaaaatac
     4141 aaaaactggc tatacaacaa ttcaatatat taggacttac ttgcgaggag aaatatagca
     4201 ccgctcaggc aattcctggg tatttacctt tcttagtttc tttactcggg atgttctgtc
     4261 atcagaaagg cgattctaaa tttgtaatat agtcactcat attcccacgt aatttcttta
     4321 gatcaatata ttgcacattt ttctcaaaaa actgatcgga aataagattt gcaacattgc
     4381 ttgttgtatc atttttaacg cttctattca tcccttcaca aagttcgtcc attttctttt
     4441 tgaaataact gcttcctgat acaagatgat ttatggaact ttgtgcagca ttgatgtcta
     4501 gagaaatgcg ataatctttc tcaacctgtt taaatatctg tttacggaga ttatcgccga
     4561 tttttcggct gtgttctgca caaaacttat caatacattg gtttaccacc cgggatacgt
     4621 agactttttt ttcatcattt ttccaatctg taatattttc accgatttgt tctgaaacag
     4681 agctcaaaat actagtattt attttggttg atgatatttt tactatcctt ttccccattg
     4741 tacagcggat aatctcatcc acctttcctg aaaaatatct tttgtgagat aaactgattc
     4801 caaaattatt aaaagatgtt ccaagcatta taatcacctt gtcatgtaaa atagtaacat
     4861 tgtcattatt ttagctgatt caaacaattc tgtaaatgct ttgagttctt tactcccttg
     4921 gctcaatgat ggaccgtctg gaatcccaga gactaaactc tctgagaaag agagtatgaa
     4981 aaaacttggg gcagtcccta aatgaaggga ccaacagcgg taaaataacc ggcatcattc
     5041 ataatgatgc cctgttagtt gtagttatga agatttacgt cattaaaatt gctgttcgta
     5101 gtgtcagtcc tatggtctgg catcagctga ggatcgtagc cgatacatca ctggcttcac
     5161 gacattcgtg ttgaggctat tcatgaaaac tccacgctga aaacgccgtt ttgtataaac
     5221 ggtcacggta tgcccggagc cactgttgct gatgaattcg ataaaacctt ggcgtttctt
     5281 gaagctatcg ttaatgcgga taatgaaaca acgattggtg agattcgctc ttttgccgac
     5341 gccctggacg ctgtccggtt caatcgcaac aaaatcaacc ggcagctgag caaacccaac
     5401 cttgcctctc tggcgctgga gcatgaagtg atttggctgg gacgtagccg ctgacagctt
     5461 ctataaaagt atgatcctac ccacgtaata tgaacacagg cttaagtgag gttctggttt
     5521 tcaaattgtt caggactgag acctccacac caactgtgcc gtcgtcagcg attgtaatcg
     5581 cacttgatat aattaaacac tgtatatttc atttgtggag tctccagtct gagagggctt
     5641 tatgcattcc gtattgggca ctatgatgcc agaaatctgc aaggctccac accttcctgt
     5701 atttcccttc ctctatcaca gtgcacttta cttaataggg tgggatgtgt tcattacatt
     5761 tattgtgttt tttatgattg tttttgcgtc cagtgtcgtt cgtcatggga aactggttct
     5821 atgggtagta tcgctcagtc tagttaactg tttgtttctt tttagaaatt aagcagatca
     5881 cacaattcgg ggagagatcc ttctaagtgc aattctttgc agctatttag cacacttaaa
     5941 acaactcttg tattttgtca gaaataatat taaacatgtc ttgttcatta ttgttaagtc
     6001 tgtctgctat ttctgtaagt tttatcccac tatgatggca tatatttata tatgccatca
     6061 tagcctcgat attccctttc cctgcggcca gaaataatcc tggtaacccc tcattatctc
     6121 ttacatccaa tagtttaatc gtttcttttg gtgataaatc aaaagtttta ataaaatctc
     6181 cataagcttg aatcgcagcg gaatgacctt cttgaagagc tatgaacaaa gcagattccc
     6241 cagcattatt tcgtgctgca agaacttcta gtttttcctc tttgggtatt tcggctgcca
     6301 acagcgattc accgtattta cgcgctgcat cggcatttcc atgccaaatc aatgaaaaca
     6361 aattatggcg gttatactcc ttattattct ttgtcggaat ggatatgttc tgtattttgg
     6421 ccaattttct gaaagaaaca ccactatatc ccccccctga tttttcttca ttgatggcgt
     6481 gggatatagc ggtatttgtc acgttaatga tattattttt cattccttta ttttcattag
     6541 cagtattgct gcctattatt aggtgcatat ttttgttgtt tgctggatgt aatccatagt
     6601 tatttattga catttttgtt catccttttt gctaaaaaaa acaataacct atttttgtaa
     6661 tgtaatagat attagtctat tgctaatcta aaaaaaccat tgccacattg aatggctata
     6721 ttcaccaata aattctttcg atttcgtata ttgacatatc gtagatatat cttatcccta
     6781 agattcaata atcgagtccg tttaccattc tgtgcaagcg ccttcactca gaagtgaccg
     6841 tcaaggcgat caccaaactt gataataaag cagctcattg ccatacgcca gtccttaagg
     6901 ggcgttgtcc atttctgtga tgctgcctag atttaactgc cagctccggc ttatctacat
     6961 ctaaatatgt gagcctaata ccgctacact ttgtccagac tatgtttttc tccgggaagt
     7021 gggctaaccg tctccataaa gtgacgaact tttttcaaca gtggccatat tgaacggcac
     7081 tgatgattac gggttattgt ctcatgaaga gccggccaca tcactgacag cagtgacttc
     7141 aggccttcag caccagacag cctgaaccag ttaatccatc gcccgacaaa tttagtaatc
     7201 tagggctggt ttttcttcag ctgttgcggt tcgaaagtgc cattacgatc acgcagagta
     7261 cggatttcca gaggttcatc tccagtagtg ataatcttcg ttaagcaacc attgcgggaa
     7321 ttgctgccag atctaagctg atttttctcg cacccaagat gatgagtcat attagcattg
     7381 agtacggttt cattttcagc atccaatcaa actgactgag atcttcagga tttttgagac
     7441 ttttggctag ttcattagct aaagcccgca acttattaga tgtcaactgt ttttcattca
     7501 taaattcacc ttcatttgat atcaaattaa atagttcaac atcagagaat tatacaaact
     7561 tatgtacagg ctcctatgat cttcgaatcc tgaagattat agtgaaaata agttttcacg
     7621 ttgaaatccc acatagttag tatatgataa ataatcatgg atgacctgat taacctcttg
     7681 gggtagtgct tttatagaag aataaatatt ctactaattt aatacatcac ctaaaatagt
     7741 attgtttttt atggtcggtt gaaggtcgtg gtgatgatgt tagtcctttt gagaatattc
     7801 cagtgccaga tcttacttat aataaagggg cttggcagtt tggataaact attgttctta
     7861 ttccactaat acaaactttt tgtaaagtat attaaatata cttcatttca tactgaaatc
     7921 cccggaagta ttcaatcaaa caattacacc ataaaaggaa gagggggccc taattaagtt
     7981 gtcaagcatg tttatgatac ctgcagggta taaattttct ggctgcaagc agacacatat
     8041 aaaacgtctt tattgatcct acccacataa tatggacaca gtcgtaagtg aggctctggt
     8101 tttcaaattg ttccggactg agaccaccac accaacggtg ccgtcgccag cgattgtaat
     8161 cgcactcgat ataactaaac actgttgccc gcactatttc ccgatggata atagtgttct
     8221 ccgtggagac attccatttt cagcgaatga attttcatct gttgctgtat ccagtcatac
     8281 agtgactgca tcagtggcgc ggctctggct tttcttgccg ccagacgctg ttctgctgaa
     8341 catccccgga cttctgcctc gatggcatac agttcaccga tacgctgcag ggcttccgtg
     8401 gtgatgtcgg ttggcactct tgcatgcaca tcgtggattt ttctccggac atgagccata
     8461 catgcggctt ccgttattct gccggattcg tacaacgccc ggtaaccacc gtaagcatcg
     8521 gcctgaagca ctccgctgta accggccaga tgattctgtg gatggatacc tttccggtcc
     8581 ggtgagtacg cgaaccagac cgccggaggc atttccgaac cggcgttacg gtcatcacga
     8641 acgtagaccc acagccgggc agtccttgtt ttaccgctgc ccggctcctg gaccggaacg
     8701 gggatatcat cggcatggac tttaccgggc atcagcacat actggcgcag gatgtcatac
     8761 agcggttcca gcagttcagc aacagcacct gtccagcgcc ccagtgtggc acggctcagc
     8821 tccacgccct gacgacggta tatttctgac tggcggtata acggcagata gtctgcatat
     8881 tttccagcaa caacatgggc cagaagcccc gctccggcat aactgcgtgc aatgggtttt
     8941 gaaggcaccg gtgcctgcac gatatggtca caccggcaac aggccagttt cggacgttgt
     9001 gtttcgataa ccttaaaggc gctgctgata agctccagct gctctgacac atcacatccc
     9061 agagaactga gttcgccacc acaggccggg cagcattcct cttccggacg gatgacccgg
     9121 gtttcacggg gaagtgaggc aggtaacggt ttacgggctg aagactggcg caggggagat
     9181 ggcagtaccg ggtcatattg ctcacccagc gtttccgcca tttcttcctg aagtgcgctg
     9241 attcgctcct gtgcttccag tatctgccgt tcggtttttg cgcgaagttt ttctgagctt
     9301 ttaccgaact gcatgcgctg cagtttcgca accagcgcct tcagccggtt gatttcggaa
     9361 gcataagccg ccacccgctg tgagagcagg cggttgtatt tcgccatctg gtggatggtg
     9421 tcctgttgcg tctgcaacag tgcccgcagg cgggcattct catgagcaag tgaggtgtcc
     9481 atatcctcac tttacaacgg gttatatgcg gatgccagcg cgttccgttc gtttcgggtg
     9541 tttccagtcg atgccctcaa gaagcatgga taactgggcc ggagtaaggt gcaccttgcc
     9601 gtcacgggtg accggccaga cgaagcggcc ccgctccagg cgtctggtaa agaggcacag
     9661 tccgtcactg tcagcccaca acacttttat ctggtcaccc cggcgtccgc ggaagatgaa
     9721 cagatgcccg gagaacgggt catccttcag gacgttctga acttttgagg ccaggccgtt
     9781 aaagccattt cgcatatcgg tgatacctgc aaccagccag atacgcgaac ctgcagggaa
     9841 agatatcatc agtggctgct cccttttatt tcgcggataa gtatctgtaa taacgccggc
     9901 gtcagtttac ctttaagcct gagagttccg gccggcagaa ccagctcaca gcacagactg
     9961 tcggacggtg tatttatctg ctctggttcc tgtgccggag ccgggatttt attatccggc
    10021 tccggcgtta acgtcacggg aagcagtgca ggcatatttt ttccggaagg cagcaggcca
    10081 cctttccggt attgatggcg ccagttgaag agcaggttat cgttgattcc gttttcacgg
    10141 gcgatctgcg ccacacaggc tccgggctgc agtgactgct ccactaaagc gattttaaat
    10201 tcataaggaa aattaggcc
//
LOCUS       DAOFMX010000122         9924 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.122, whole genome shotgun sequence.
ACCESSION   DAOFMX010000122 DAOFMX010000000
VERSION     DAOFMX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.122"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..712
                     /locus_tag="QBA20_003743"
                     /pseudo
     CDS             1..712
                     /locus_tag="QBA20_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            725..1638
                     /locus_tag="QBA20_003744"
                     /pseudo
     CDS             725..1638
                     /locus_tag="QBA20_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2317..2535
                     /locus_tag="QBA20_003745"
     CDS             2317..2535
                     /locus_tag="QBA20_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDQ6294116.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2753..2995
                     /locus_tag="QBA20_003746"
     CDS             2753..2995
                     /locus_tag="QBA20_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294117.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3325..4254)
                     /gene="fdhE"
                     /locus_tag="QBA20_003747"
     CDS             complement(3325..4254)
                     /gene="fdhE"
                     /locus_tag="QBA20_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDQ6294118.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4251..4886)
                     /gene="fdoI"
                     /locus_tag="QBA20_003748"
     CDS             complement(4251..4886)
                     /gene="fdoI"
                     /locus_tag="QBA20_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDQ6294119.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4883..5785)
                     /gene="fdoH"
                     /locus_tag="QBA20_003749"
     CDS             complement(4883..5785)
                     /gene="fdoH"
                     /locus_tag="QBA20_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDQ6294120.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5798..8848)
                     /gene="fdnG"
                     /locus_tag="QBA20_003750"
     CDS             complement(5798..8848)
                     /gene="fdnG"
                     /locus_tag="QBA20_003750"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8261..8263),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDQ6294121.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9042..9875
                     /gene="fdhD"
                     /locus_tag="QBA20_003751"
     CDS             9042..9875
                     /gene="fdhD"
                     /locus_tag="QBA20_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDQ6294122.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2489 a         2396 c         2542 g         2497 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aaagtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaaagttgag gatatgtgtg aaagaatgaa aggtggcatc tctatgttta accacaccac
      541 aaaaacagct gataatatag agcataattt gcagctaagt tttctcgtgg gttcacgtcc
      601 gtcgatcacc aataaatact cagcaaaaga tgtaggtcca gatatacttg cattgcccgt
      661 caacgtatat catgcggatt ttaaaattaa aatcaataat gcagtggaga aggcttcatc
      721 aattgtgtta tgtgctgtgc aaaaaaatct ccatgcagca aggaaggttc ttagcgggat
      781 aataggaaat gtgccaaaaa ataataaacc aaaaacaatt atttttgatt tgcgttttct
      841 tagtgcaatt aagcagaaag ttttttgggc agtgaaaaac cgttgtttgt aaagcatacc
      901 gcaatgatga gggaggcatg taaggaatta ccgcagtcag tggaatatgt tcctttagcc
      961 tgtgaagcgc atagccatcg cagcgtcgcc cttgccaaaa ccgtggctgc gcccatcatg
     1021 gcgacaggtg tgggcttggc attaatgtat gcaacatcat caggaccgta ttatgcgcaa
     1081 agtctttctg gttcgccgga tatcgagatg ataaaaaatc gcatggctga attgtttaac
     1141 cagtcggata tttctgtgcg caacaaatat agcccggctt ttgataaatg gaatgaactg
     1201 gtagataaat taaatcacga aagaaataca gttccgtttg cgtgtttgac caccatttta
     1261 gcgacggcga taaatgaaac tcttgagggg gatacgaatg ttgccgttgt gatggggtgt
     1321 aaatctgcga aagatcgcac gatatccatt gtccttggca actcgatgtt gcaaacactc
     1381 cttgaaaaac gcctcgccga tagtggagaa atagaaaatc tttttgatca acagggatat
     1441 tttagttgcg attcattaac acccgaagaa ttaatgatgt taaaagacct ttttgatatt
     1501 cgagttcttc atgtctctaa caaattcaat gttggattgc aaggtaatat caacactgat
     1561 gttttacaag attcgttttt taaaaatgtc gatttcattc atgaatataa ctcgtatacc
     1621 gtagggatgg gagtctgacg agtaaaactc attaggatta tcagaattcg gacaatgcat
     1681 tgcaaaaatg acatacaaag agagatgtgt gcaggtactt agccgcgagg ggcatggcga
     1741 agccatacag aaagtaccag tattcagaac tccagtgaaa gcggcatagt ggccgcctgc
     1801 tcaggagttt tgctatgacc cgtaatcgtg ctgaacgtcg ccaccctatg atgcgattaa
     1861 aaaaactcat cgtcgcgctg atgattgacg tcaatgctat cggatgatgc cgggggtgtt
     1921 ccggaaggaa ttccttgttt cccgaactgt taccaacaaa attataacga tgagcaagta
     1981 aagaccggtt ggtggcagta ctgaccggtc tgtgatgaaa gattaatttc accttaaatc
     2041 ctggtgctgt taggatgctg acgtttttcg gctacaccat tcagattatg caaataaata
     2101 tccctgaata taatatattc atagcaccca taactcatcc tgggcgataa ctaaaccatt
     2161 tttgagtaat aaatgaaaca actgcaacgc tcttattatt tctggaaaac gcctcgcaaa
     2221 aacaaaatga catgcaaaaa tccgcatttt tttaaatgct tttccggcgt aattttcgca
     2281 catacattaa tgagtaatat atgtaaggtg gtggtaatgg cgatgaatac ggtttttctt
     2341 catttatcag aagaggcaat taaacggctg aacaagcttc ggggctggcg taaggtttca
     2401 cgatctgcaa ttttacgcga agcggttgag caatatctgg agcggcagca atttccggtg
     2461 cgtaaagcaa agggaggcag gcaaaagggg gaggtagttg gtgttgatga tcagtgtaag
     2521 gagcataagg aatgaaaaaa atttttcatt cttgaatata aaaaacagat gcccttattc
     2581 tggtattaat acaaggctgt tttacttgaa cttataataa ctgcaactgt tacatcgtat
     2641 ctggaaaacg cctcgcaaaa ataaaaaatg atgcgtaaat gagctttctt taaagattcg
     2701 gcaagggtac ttttctctcc atagattcaa aaatgatatt ggcgagatat ttatgaactc
     2761 tctggcaggt atagatatgg gcagaatttt actcgattta tcgaatgagg tgattaagca
     2821 acttgatgat ctcgaggtgc agcgtaatct tcctcgtgcg gacctattaa gggaagcggt
     2881 agatcaatac ctgataaatc aatcgcaaac agcaagaacc agtgttcctg gcatctggca
     2941 agggtgtgag gaagatggtg tcgaatatca gcgtaagctg cgcgaggaat ggtaatggga
     3001 aaaaatgatg tcaatcagat tgctgacaac gtgcgcgttg ttcatgccgg atgcggcgtg
     3061 aacgccttat ccggcctaca aagtcatgca aattcaatat attgaagttt attggtaggc
     3121 ctgataagcg cagcgcatca ggcaatcata cgcttgtcat cagtgacata cccataacct
     3181 tatcgatgtg ttggagggaa gaacgaggca aacagacaat tgcggcatga tcttgaatgg
     3241 gtaacgcgga atacgagaga tggtgtcgtc acaccctatg agctgtaatc cgggcgacct
     3301 gcgccacccg gaaaacgtca gagattactc tccttcaccc ggaaacagga acgggttaat
     3361 ggaactacgg gcgtagcctt cttgctccat tcgcgcgtcc agtaccagag aggcgaggtc
     3421 atctgccacg gcttcaactt tcggttcttt ttcctgataa agaatcttca ggtaagtgcc
     3481 gcagtcatcg cagctttcgg ctttaatcgc ggcctgttcg tcatccagcg accagtaatg
     3541 cagtttgccg ctctgctcgc agttgctgca ttttacgcgc actacgtgcc attcggtttc
     3601 acacaggttg cagtgcaggt aacgcagacc ctgagtggtg ccaatttgca ccatgctgga
     3661 caccggcata gagccacata ccgggcaata ttgacgttgt tcgccgtatt cagcgcgggc
     3721 tttgccgggg atcagattgg ccatctgcgc ccagtagagc gacagcgcag cccagataaa
     3781 cggtgcttta tcgctgctga cggacgagaa atcagaggca aacagcgcgc tggccatatc
     3841 ttccagctcc tgagtcgatg ccttctccag attctcaatc actgccagcg ccgggccgct
     3901 catttcaggt ttcagctcag caattagcgc catcagcagc ttttgccagt gcttatcacg
     3961 cggcagaacg tgaatatcca gcgggggctt gccctgtgcg ctggcttctt taatgcgcgc
     4021 ggtcagatcc atctccagcg gatggtcgta cagcaccact ttctgggcgt gggcgataag
     4081 cgcagcaaag cgcaggtaat cacccagcgg attattttct gccagctcgc gcagacgctc
     4141 ggcgcggcgg ttgtataaat tcttgagccg agggaacaat aacggcggaa tcatatccgc
     4201 cgtacgtttc tcgctcgaac ccagctcatc ttgcgggatt atgcgaatac tcattcagct
     4261 ttcttttccg ttgtcttgcg gacctcacgg taccagcgcg ggtgatgttt cttcgcccag
     4321 gcgctggtaa cccatccttc caccatggcg gtaatcgtgc ctttcaccca aagggcggcg
     4381 tagatatgca ccatgataac cacaattaac gccactgcgg caaatgaatg cagcattaac
     4441 gcgaatcgga tcaccgggat tgagaaagca ggcgcaaaat aaggacgcca gataatcacg
     4501 ccgctcacca gcagcagaac caggaaaata atcgccgccc agaaaacgca tttctgaccg
     4561 aagttataac gcccggtgtc acctacttcc tcgttgacga cgatcttacg aatattcttc
     4621 gcccaaaaga tatcatcccg attgattagg ttttgatgcc agtaacggaa aaacatgatg
     4681 atgaacgagg caaacataac cacgccgaca aacgggtgca gaattcgcgc cagctgcggt
     4741 gtgcccatga tttgcatcaa ccagttgaag gacgggaaca aaaagcccag cccgctcacc
     4801 gccgccagga tgaagcagaa ggcggtgatc cagtggttga tacgttccgg cgcggtgtag
     4861 cgcacgatgg tgtcacgtcg tttcatttgc gctcctcgtc tttctcttcg tgcagattat
     4921 tctcttcctc atccgcacgg ttcggaccga cacccacgta gtggaagata ctggccgcaa
     4981 aggtagccgc aaagccaaca gccgcgagcg gtttccagat gcctttccag aatttcacgg
     5041 tttcgctgat ttccgggttc tccggcaagc catgatacag atttggcttg tcagcatggt
     5101 gcagcacgta catgacgtgt gtaccaccga cgcctgccgg atcgtacaga cccgcattgt
     5161 cgtaaccgcg ggttttcagc tcagcaacgc gctcgctcgc cagcgttttc atcgactctt
     5221 tcgtgccgaa gtgaatcgcg cccgttgggc aggtcttcac gcaggctggt tcttgcccaa
     5281 ccaccacgcg gtcaacgcac agcgtacatt tgtagacgcg gttgtcttcc gggttgaggc
     5341 gcggaatgtc gaacggacag cccgcaatgc aataaccgca gccaatgcac tgctcggact
     5401 ggaagtcgac gataccgttg gcatactgaa taattgcacc ttccgccggg cacgctttca
     5461 ggcagcctgg atcggaacag tgcatacagc cgtctttgcg gatcagccat tccagtttgt
     5521 cgttctgctc cacttccgag aagcgcatta ccgtccacga tttggcgctt aaatcattgg
     5581 ggttgtcgta caccccaatg ttattgccga cggtatcgcg aatgtcgttc cactctgaac
     5641 acgccacctg acaggcttta cagccgatac aggtggtaac gtcgatgagt ttcgccactt
     5701 cttcctggaa gtcccgcgcc tgaggcgcgg gggtcagacc gttagtcgcg gaacgacgaa
     5761 tgatatcttg agattgataa gccatatgtc gtctccgtta caccttttcc acattcacaa
     5821 ggaaggactt aaactccggc gtctgcgtgt tcgcatcacc aacgaatggc gttaaagtat
     5881 tggcaataaa gcctttttta gctacgcctt catagcccca gtgaatcgga ataccgatgg
     5941 tatcgatatc tttgccgttc gctttcagcg tgcgaatacg tttggtcacc accgctttgg
     6001 ctttgatata gccacggttg gaggagactt tcacggtatc gccctgggca atgccaagtt
     6061 tattcgccag cgactccccg atttccacaa actgctctgg ctgcaaaatc gcgttcaaca
     6121 gcgcgtgttt ggtccagtag tggaagtgct cggtcagacg ataggtggtt ccgacatacg
     6181 ggaacttatc ggctttaccc aatgcttcgg cgtcgtcttt aaagatacgt gcagctgggt
     6241 tcgagataac gtttggatgc agcgggttag ttcccagcgg cgtttcaaac ggctcgtagt
     6301 gttccgggaa cggaccttcc gccatcttat cgagggcaaa cagacgcccc atgccttcct
     6361 gctgcatgat aaacggcccg acgccgctac ccggaggcgc tgcgctgtag tccggaatat
     6421 cccagccggt ccacttagtg ccgtcccatt tcagcaactg acgcttcgga tcccacgggt
     6481 taccctgcgg atctgcggag gcgcggttat acagaatgcg gcggttaagc ggccatgccc
     6541 atgcccagcc cagcgtgtta ccgaggccag acggatcggc gttatcacga cgcgccatct
     6601 ggttgccttc cggcgtccag ctaccggcga aaatccagca gccacaggag gttgtaccgt
     6661 catcgcgcaa ttgggcgaac gagctaagtt gttggccttt cttgacgata accgcaccgg
     6721 ttgccggatc ggtaatatcg gccagcgcct taccgttgct ctccatcgct acttcttccg
     6781 acttcggctc atgcggaatg gcgtagttcc aggtcatgtt cagcacctga tccgggttcg
     6841 cgccaccctg ttcggcatac atcttgcgca agcgcaggaa gataccggag aggatctcac
     6901 cgtcagtcag cgcaatcccc ggggcgtccg cacctttcca gtgccactgc aaccagcgac
     6961 ctgagttaac gattgaaccg ttctcttctg cgaagcaggt cgatggcaga cggaacactt
     7021 cggtctggat cttcgacgag tcaacttcgt tcagctcacc gtggttctgc cagaagttag
     7081 aggtttcagt gttcagtggg tcgatggtga cgaggaactt cagtttcgac agacaaccga
     7141 tcactttgtt tttgttcggg aatgaggcaa cagggttaaa gccctggcag atatagccat
     7201 tgaccttgcc ctctttcatc atctcgaagt attgcaggac gtcgtagcct ttatcccact
     7261 tcggcaacca gtcaaagccc cagctatttt ccgccgtcgc tttatcacca aagaaggcct
     7321 tcatcataga gacgaagaat ttcgggtagt tgccccagta gtttacctgg ccttccagca
     7381 gcggttttgg cgtgttggcg gtaaggtagg tttgcagatc ggtctgcttc tcgcttggca
     7441 gcgtcatgta acctggcaga ctctgcgaca gtagccccag gtccgtcagc ccctgaatat
     7501 tggagtgacc gcgcagggcg ttaacgccgc cgccggccat ccccatattg ccgagcagca
     7561 gctggatcat cgccatcgta cgaatgtttt gcgcaccaac ggagtgttgc gtccagccga
     7621 gggcatacag gaacgaggca gttttatcgt gagcactggt ttctgcgatg tattcgcaga
     7681 ctttcaggaa cgcgtctttt ggcgtaccac agatgttttc aaccacatct ggcgtgtagc
     7741 gggaaacgtg ctgtttcagc aagttccaca cgcagcgcgg atgttgcagc gtggtatcgc
     7801 gtttggcgaa gccgttttcg tccagttcat aagtccagga ggatttatcg tacttgcgtt
     7861 tttccgcgtc gtagccggtg aacaggccat cttcaaagcc gtaatcctca cgcacgatca
     7921 ggctggcgtt ggtataggct tcggtgtatt cgtggttgaa tttttcattg ttcagcaggt
     7981 acagcaatac gcctgacagg aaagcaatgt cagtaccgga acgaataggg gcatagtagt
     8041 cagctaccgc cgccgtacgc gtaaagcgag gatcgatcac aatcagcttc gcgccgttgt
     8101 gaattttggc ttccatcgcc cagcggaacc cgaccgggtg agcttcagcg gcgttaccgc
     8161 ccatcaccac gacgaggttg gcgttcttga tgtcgaccca gtggttggtc atcgcaccgc
     8221 gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacacgc gcctggttgt
     8281 cgaccgcgag catacccagc gcgcgggaga atttttgcgt taaatagccg gtttcgttac
     8341 tcgacgcgga agcacacagc atcccggtgg agagccagcg gttaacagtc acgccttcgg
     8401 cgttttgcgc aatgtagtta gcatcgcggt cttctttcat cagtttggcg atgcgatcaa
     8461 acgcctcttc ccaactgatt tgctgccatt tatcagaacc tggcgcacgg tattccggaa
     8521 acttcagacg gctttcggag tggatgaaat ccaccaggcc agcgcctttc ggacaaagtg
     8581 caccgcggtt gaccgggtga tccggatcgc cttcgatatg gaagatagat gctttggcgt
     8641 tttttgctcc gtcaccgagg ctgtacatca acagcccaca gcctacggaa caataggtgc
     8701 aggtattacg ggtttcgcgg gtgcgcagca gtttatactg ccgggtttcc gcgagtgcta
     8761 cgctgggtgc aaaacccagt gccgctgccg tggtgcctgc cataccgcca gcgcagatct
     8821 taaagaactg ccttctgctg acctgcatgg attgctcctt gtttcgacat tgtcacgtcc
     8881 catttacatt cgcttgctgc gtgtgcaggg agtgggagtt atttttcttt gcggaagggg
     8941 ccgcaaaggt ccagaattgg ctcaatttcc ctccatctag gaagggtttg taacagaata
     9001 ccataatgtt ggtgtgtatg ttcttatctg gttaagagaa agtgaaaaaa acacagcgaa
     9061 aagaaatcga aaatgtgaca aatatcacag gtgttcgtca aattgagtta tggcggcgtg
     9121 atgatttaca acacccacgg ttagatgagg tcgcggaaga agttcccgtt gcgctggtct
     9181 acaacggcat ttcgcatgtg gtgatgatgg cgtcgcccaa agaccttgag tactttgcgc
     9241 tcggtttttc gctttccgag gggataatag aaagtccgcg cgatatcttc ggcatggatg
     9301 tcgttccttc ctgtaatggt cttgaagtac aaattgagct ttccagccgc cgctttatgg
     9361 ggttgaagga gcgccgccgg gcgctggcgg gacgtacggg ctgtggcgta tgcggcgtgg
     9421 agcaacttaa tgacatcgga aaaccggtgc agccgctacc gttcacccag acgtttgatc
     9481 tcaacaaact ggatgatgca ttacgtcatc tcaacgattt tcagccagtg gggcgactga
     9541 ctggttgtac tcacgccgct gcctggatgt tgccatctgg cgaactggtc ggcgggcatg
     9601 aagacgtggg tcgccatgtg gcgctggaca aactgttagg ccgccggtca caagaagggg
     9661 aaagctggca gcaaggtgcg gtactggttt ccagtcgtgc cagttatgaa atggtgcaaa
     9721 agtcggcgat gtgcggcgta gagattttgt ttgcggtgtc tgccgcgacc acgcttgctg
     9781 tagaagtggc tgagcgctgt aatctgacac tggtaggttt ttgtaaaccg ggtcgggcaa
     9841 cggtttatac ccatccgcag cgtttgagca attaattaaa ataagtcctg cgatatatat
     9901 taaattgcag gacttattca tttc
//
LOCUS       DAOFMX010000123         9905 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.123, whole genome shotgun sequence.
ACCESSION   DAOFMX010000123 DAOFMX010000000
VERSION     DAOFMX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.123"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="QBA20_003752"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="QBA20_003752"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDQ6294123.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="QBA20_003753"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="QBA20_003753"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDQ6294124.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="QBA20_003754"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="QBA20_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDQ6294125.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQIADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4692..6452)
                     /locus_tag="QBA20_003755"
     CDS             complement(4692..6452)
                     /locus_tag="QBA20_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDQ6294126.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSKWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6582..7208
                     /locus_tag="QBA20_003756"
                     /pseudo
     CDS             6582..7208
                     /locus_tag="QBA20_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7407..8495
                     /gene="serC"
                     /locus_tag="QBA20_003757"
     CDS             7407..8495
                     /gene="serC"
                     /locus_tag="QBA20_003757"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDQ6294127.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVSSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8566..9849
                     /gene="aroA"
                     /locus_tag="QBA20_003758"
     CDS             8566..9849
                     /gene="aroA"
                     /locus_tag="QBA20_003758"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDQ6294128.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2523 a         2364 c         2481 g         2537 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt actaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc gatttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa
     4441 taaattttaa aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta
     4501 attaaaaggg agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa
     4561 ttaccgatat ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg
     4621 ggcattaaaa aaacgccccg taatataact cagactaatc aaatagccta cattgcgtag
     4681 gctatttgat tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc
     4741 ttcaacaacg actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg
     4801 taaaacgccg cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac
     4861 atgcgaacaa acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat
     4921 aacaacgttt gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta
     4981 aactccatcg tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc
     5041 agcatggctt ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc
     5101 agacccaaca gctcacgcac gcgggtaaag tcatcaaaat cttcttcatc cagttgctcg
     5161 atgaggttca ggtaatcttc tttttcccac ttgctgcctg gtagcgaaag aatcgtttca
     5221 cgtaaatggc tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg
     5281 gacatgccag gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata
     5341 taaacttctt tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg
     5401 gtgccggaga aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag
     5461 gagattaaac cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct
     5521 tcatcatcga aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc
     5581 agctcggtca cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca
     5641 aagcaggtgc cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca
     5701 agggaaccat catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca
     5761 accaccgctg ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca
     5821 gcaataatgc ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct
     5881 tcgttgcggg tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc
     5941 atcggaatat aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca
     6001 cgatcttcgt taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga
     6061 tcgtaaaatg cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc
     6121 agtgggaacc atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc
     6181 agccagaaat cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca
     6241 gaagccagcg ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct
     6301 ttgtcgcgaa tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca
     6361 atctgaaagc cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg
     6421 gcatctttgc cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg
     6481 cgcaataata cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt
     6541 gactatgctt ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc
     6601 accgtatggc atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc
     6661 tttatacctt tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca
     6721 gatgtcgctg tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc
     6781 gttttatgat gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt
     6841 ataccgcctg gtaatatgat ggcgagctgg cctggtcgaa actcaataac tccaacatga
     6901 cggaatttga gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac
     6961 gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg
     7021 tgaaaccggg tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg
     7081 agtcggcgga ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa
     7141 aacaattatg tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg
     7201 tgacctgaca gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat
     7261 gcaagccaca tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata
     7321 aaaccgatag ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta
     7381 atagcaacgc aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg
     7441 caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc
     7501 ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg
     7561 aagccgagaa ggattttcgc gatcttctta atgtctcctc caactacaag gtattattct
     7621 gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa
     7681 ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa
     7741 aatactgcac gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta
     7801 agccaatgcg tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg
     7861 aaaccatcga cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg
     7921 ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa
     7981 tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg
     8041 aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca
     8101 acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg
     8161 tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa
     8221 aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga
     8281 aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat
     8341 tgttccttga agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg
     8401 gcggaatgcg cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag
     8461 acttcatggt tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac
     8521 agccagcctg tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg
     8581 ttacaaccca tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct
     8641 aaccgcgctt tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg
     8701 gatagcgacg acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg
     8761 ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa
     8821 ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct
     8881 ctttgtctgg atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg
     8941 attggtcatc tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa
     9001 gaaaattatc cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat
     9061 ggctccgttt ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa
     9121 gatacggtga ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc
     9181 aatctgatga agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta
     9241 aaaggcgggc agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg
     9301 gcttcttact tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt
     9361 ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg
     9421 accatttgct ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat
     9481 atggatatga accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca
     9541 aaaggcacca ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg
     9601 tttgcgatgg caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac
     9661 attcgtatca ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac
     9721 cggatggcga tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat
     9781 cccaaatgca cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag
     9841 gcagcctgaa tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca
     9901 cattg
//
LOCUS       DAOFMX010000124         9808 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.124, whole genome shotgun sequence.
ACCESSION   DAOFMX010000124 DAOFMX010000000
VERSION     DAOFMX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.124"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..213)
                     /locus_tag="QBA20_003759"
     CDS             complement(<1..213)
                     /locus_tag="QBA20_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HDQ6294129.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSS"
     gene            704..835
                     /locus_tag="QBA20_003760"
     CDS             704..835
                     /locus_tag="QBA20_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HDQ6294130.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            1095..2090
                     /gene="pgl"
                     /locus_tag="QBA20_003761"
     CDS             1095..2090
                     /gene="pgl"
                     /locus_tag="QBA20_003761"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDQ6294131.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2131..3084)
                     /locus_tag="QBA20_003762"
                     /pseudo
     CDS             complement(2131..3084)
                     /locus_tag="QBA20_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3290..4321
                     /locus_tag="QBA20_003763"
     CDS             3290..4321
                     /locus_tag="QBA20_003763"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDQ6294132.1"
                     /translation="MMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGNDLEIDGIG
                     GGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGAFAIENGL
                     IAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVALTFLNAA
                     GTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELDADKALLA
                     RIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRALAITGAI
                     AISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRASVIRTTRK
                     IFSGEVYLP"
     gene            4397..5830
                     /locus_tag="QBA20_003764"
     CDS             4397..5830
                     /locus_tag="QBA20_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDQ6294133.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            6013..8193
                     /locus_tag="QBA20_003765"
     CDS             6013..8193
                     /locus_tag="QBA20_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDQ6294134.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8334..9617)
                     /locus_tag="QBA20_003766"
     CDS             complement(8334..9617)
                     /locus_tag="QBA20_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDQ6294135.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
BASE COUNT         2425 a         2261 c         2470 g         2652 t
ORIGIN      
        1 cgaggaaagc gccaaggctc gcaggtccag tttatgcccc ttagaggtaa gacatgcacg
       61 aagtcttctt accgcctcag tacgctgttc ttccggtaac ccctgtttct cccaatcatc
      121 ccattcggac aaataatggt tgggggatgt aattgtgctg atatgtggaa ataaacagga
      181 tgaaatgttt ctgagcatat taatttctcg catatggata tggaattaga ttgtgaataa
      241 attgtattta atgtatctct cctaatgagg agggtgtttc agaatattcg ccataaggga
      301 atgagtgtta tatcatgctt tctaccctgg taagtatatt tgcttttttc gtatatttgg
      361 ttcatctttt tttgatgttt tacatattca caatgcagag gatttatacc agaggacatt
      421 agtcttgagt aagatactac aacatctcca actattttag ccagtctgac tacttgactt
      481 ggtaatattt gttgctaata atcatgtata aaataagctt atatacatag taacttcagc
      541 aaaggattta gtaatgatca aaatcatatc tcaatgtact gtttttttct ttaaaaaaac
      601 agtacattct attttataat gactgtgtat tcccatggaa ggagatcacc caattctccc
      661 ccccgacttt tgacgcagtg tcttgttaac agggggattg accatgctta ttggctatgt
      721 acgcgtatca acaaatgacc agaataccga tctacaacgt aatgcgttta tttgtgccgg
      781 aagtgagttg attgttgcgc gaatgggaag ttggccagaa gttgttatgg catagtgttt
      841 acggtcgttg atacaggtaa acaaaagcgg ggagagtcat actccctgaa tagtcgcgaa
      901 aatatctgtg gcattgtcaa attcgtgtcg gtgactaata ttttagtaat atgccattcg
      961 aaatggagtg ctaacttatt gatttaattg atgtgcgata ttgtatttta tcgtgttttg
     1021 ttgtctgttt tttatccagt aagtgttgtt atatgtatga aagataaggg atttatcatt
     1081 tttgaggtgt gttcatgaag caaacagttt atatcgccag ccctgagagc cagcaaattc
     1141 acgtctggaa cctgaatcat gaaggcgcac tgacgctgac acaggttgtc gatgtgccgg
     1201 ggcaggtgca gccgatggtg gtcagcccgg acaaacgtta tctctatgtt ggtgttcgcc
     1261 ctgagtttcg cgtcctggcg tatcgtattg ccccggacga tggcgcactg acctttgccg
     1321 cagagtctgt gctgccgggt agtccgacgc atatttccac cgatcaccag gggcagtttg
     1381 tctttgtagg ttcttacaat gcgggtaacg tgagcgtaac gcgtctggaa gatggcctgc
     1441 cagtgggcgt cgtcgatgta gtcgaggggc tggacggttg ccattccgcc aatatctcac
     1501 cggacaaccg tacgctgtgg gttccggcat taaagcagga tcgcatttgc ctgtttacgg
     1561 tcagcgatga tggtcatctg gtggcgcagg accctgcgga agtgactacc gttgaagggg
     1621 ccggcccgcg tcatatggta ttccatccaa acgaacaata tgcgtattgc gtcaatgagt
     1681 taaacagctc agtggatgtc tgggaactga aagatccgca cggcaatatc gaatgtgtcc
     1741 agacgctgga tatgatgccg gaaaacttct ccgacacccg ttgggcggct gatattcata
     1801 tcaccccgga tggtcgccat ttatacgcct gcgaccgtac cgccagtctg attaccgttt
     1861 tcagcgtttc ggaagatggc agcgtgttga gtaaagaagg cttccagcca acggaaaccc
     1921 agccgcgcgg cttcaatgtt gattacagcg gcaagtatct gattgccgct gggcaaaaat
     1981 ctcaccacat ctcggtatac gaaattgttg gcgagcaggg gctactgcat gaaaaaggcc
     2041 gctatgcggt cgggcaggga ccaatgtggg tggtggttaa cgcacactaa ccgctgattt
     2101 aaccggcgca gtctctcctg cgccggtgta ttaacttatc tcctgtaacg catgtctctg
     2161 gcgttcgacg atattggtcc acaaattgtc tttatcgtca gtccacaaat taatcagcaa
     2221 ggaaaaaaag cgttcagctg ccggagaaag cacggcatct ttgcggcgaa taatccccaa
     2281 tgtccgacgt ataacgggtt ccaccagtgg gataccaata atcgaagaat agggcgcatg
     2341 gggcattgcc agaccaggaa gcgccgaaat ccccagtcct gcctccacta gtcctaatga
     2401 cgtcgaaaga tgacgcactt cgtaaaacca gtccagcttc cagggcttgt cggctagctg
     2461 ttgttctatc aacaatcggt tgccgctgga ggaacgtacg ccaatcattt tgtaacccac
     2521 taactcctgc cattctacaa gctacttttt ggccaatgga tggtcacgcc gacaggccag
     2581 cacgaatggc tcgttgacca gtggggtaaa atcaatggat gaatttgtga cgttgttcat
     2641 gtttatgcca aagtctgatt cgttacacag caccgattcc atgcaattat tcgtgccttg
     2701 ttccagaatc cgcactttaa tattgggata cagctcatta aatttaccga ttgccagcgg
     2761 taaaaaataa aatactgcgg tcggaataca cgctaatgtc accataccac gatgataagc
     2821 attcatatca cgaatattaa aaagcgtttc atcgaatttc tttattaatt cccgcgcctc
     2881 cgggagcaac ctttttcctg cttttgttaa cgtaaccttg cgagttgtac gctcaaatag
     2941 ttgaatatgt aaatcttcct ccatcttttt tattcttcgc gttaatgcag gttgcgtaat
     3001 attgagtaat ttagcggcat tgttaaatga actggactct gccagtatga caaatgcctt
     3061 catacttgat aattcatgct tcattattac tccggaaaat ggaagcgacg attttggggg
     3121 gctggtcgtt aaaaatttta actgcattta gccaacttaa attaatgaaa aaatgttatt
     3181 aatcgttgag ctaaagtcat tagagatgct ttgtccttaa tgtaaccata tcgcaataag
     3241 ttatgttttt aaattgaggg cattattatg aaaaaaaata ccctgcgtga tgatgcgagg
     3301 tggaacctcg aggggcgcgt tcctgttagc ggaacattta cccgaagatc aaacgcagcg
     3361 cgataaaata ttgatggcaa ttatgggttc cggtaacgat ctggaaattg acggtattgg
     3421 cggcggtaat ccactgacca gtaaagtcgc cattattagc cgttccagcg atccgcgtgc
     3481 tgatgtcgat tatctgtttg ctcaagttat cgtccatgag caacgtgtcg ataccacgcc
     3541 taactgcggc aatatgctgt ctggcgtagg ggcattcgcc attgaaaatg gtttgattgc
     3601 agcgacttcg ccagttaccc gtgtacgtat ccgcaacgtc aatacgggta cgttcatcga
     3661 agctgatgtg caaacgccaa atggtgttgt cgagtacgag ggtagcgcca gaattgacgg
     3721 cgtaccgggt actgccgcac cggttgcgct cactttcctg aatgccgctg gaacgaaaac
     3781 cggaaaagtt ttcccgactg ataatcagat tgattatttt gacgatgtcc cggtgacctg
     3841 tatcgatatg gcgatgccag tcgtcattat tccggctgaa tatctgggaa aaacaggtta
     3901 tgaattaccg gcggaactgg atgccgacaa agcattatta gcccgcattg aatctatccg
     3961 gctacaagcg ggtaaagcaa tgggcttagg tgatgtcagt aatatggtta tccctaaacc
     4021 tgtgcttatt tctccagcgc agaaaggcgg ggcaattaat gtgcgttatt ttatgccgca
     4081 ttcttgccat cgcgcgctgg cgataaccgg tgctattgct atttccagta gttgtgcatt
     4141 ggaaggcacc gtcacccgac aaatcgtccc ttctgtagga tatggcaata tcaatattga
     4201 acaccccagt ggtgcgctcg acgttcattt aagtaatgaa ggtcaggatg ccacgacgtt
     4261 acgcgcatct gttattcgga caaccagaaa aatattttcc ggtgaagttt atcttccctg
     4321 aaaaaattcg ttgtcaggat aaggacaatc aataaaggac ttctgtatga gtcatacaga
     4381 aagaacagga ttttaaatga ataagaaatc gttatggaag ctaattctga tattagcgat
     4441 cccatgtatt attggtttta tgccagctcc ggcaggatta agcgaactgg cgtgggtgct
     4501 ttttggtatt tacctggcgg ccattgtggg tctggttatc aagcctttcc cggaacctgt
     4561 cgtactgtta attgccgttg ctgcctcaat ggtggtggtc ggtaacttat ccgacggtgc
     4621 gtttaagacc accgccgtat taagcggtta ctcttcaggt actacctggc tggtgttctc
     4681 ggcgtttacc ttaagcgccg catttgtgac caccggttta ggtaaactta ttgcctatct
     4741 gctgattggt aaaatcggta acaccatgct gggtctgggt tacgttacgg tattcctcga
     4801 tctggtactg gctccggcaa caccgtctaa caccgcgcgt gcgggcggca ttgtgttacc
     4861 gatcatcaac agcgtggcgg tggctttggg gtccgaaccg gaaaaaagtc cgcgtcgtgt
     4921 cggacattac ctgatgatgt ccatttacat ggtcaccaaa accaccagct atatgttctt
     4981 taccgcaatg gcggggaaca ttctggcgct gaaaatgatc aacgacattc tgcacctgca
     5041 aattagctgg ggtggatggg cgctggccgc cggattgccg ggcatcatta tgctgctggt
     5101 caccccgctg gtaatttaca ccatgtatcc accagaaatt aaaaaggtgg ataacaaaac
     5161 catcgctaaa gcgggccttg ccgaactggg accgatgaaa atccgcgaaa aaatgctgct
     5221 cggtgtcttt gtgctggcgc tactgggctg gattttcagt aagtctctgg gggttgatga
     5281 atccactgtg gcaatcgttg ttatggcgac catgctgctg ctgggtatcg ttacctggga
     5341 agacgtggtt aaaaataaag gcggctggaa taccttaatc tggtacggcg gtattatcgg
     5401 cttaagctcc ttattatcga aagttaaatt tttcgaatgg ttagctgaag tctttaaaaa
     5461 taacctggca tttgatggtc acggtaacgt tgctttcttc gttattattt tcctcagcat
     5521 catcgtgcgt tatttcttcg cttccggtag tgcctatatc gttgctatgt tacctgtatt
     5581 tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta accgcgctgg cactgttgtt
     5641 ctccaactcc tatggcggca tggttactca ctatggcggc gcggcaggtc cggtcatctt
     5701 tggcgtgggt tacaacgata ttaaatcctg gtggttggtc ggtgcggtac tgacgatatt
     5761 aaccttcttg gtgcatatca ccctcggcgt gtggtggtgg aatatgctga tcggctggaa
     5821 catgctgtaa atatactcgt catacttcaa gttgcatgtg ctgcgtctgc gttcgctcac
     5881 cccagtcact tacttatgta agctcctggg gattcactca cttgtcgcct tcctgcaact
     5941 cgaattattt agagtatatc catttattat ctttctgcgc acttcacggt gcgcagatat
     6001 ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt ttctcgccag taataacgaa
     6061 ataattgccg aagaacattt caccggcgaa attaaaaaag aagaagcaaa aaaaggcact
     6121 attgcctggt ctattctctc ttctcataat acgtccggaa atatggataa acttaaaatt
     6181 aagtttgatt cattagcctc tcacgatatt acctttgttg gtattgtaca gaccgctaaa
     6241 gcgtccggaa tggaacgttt cccgctgccg tatgtgctga ccaactgcca taactcactc
     6301 tgcgccgtcg gcggcaccat taacggtgat gaccatgttt ttggtttatc ggcagctcag
     6361 cgttatggcg gtatttttgt gcctccgcat attgcggtca tccatcaata tatgcgtgag
     6421 atgatggctg gcgggggcaa aatgatcctc gggtcagaca gccacacccg ttacggtgca
     6481 ttagggacaa tggcagtcgg tgagggtggc ggtgagttgg taaaacagct gcttaatgac
     6541 acctgggata tcgactatcc gggcgtggtt gcggtgcatc tgaccggaaa acctgcgccg
     6601 tatgtggggc cacaggatgt ggcgctggct atcattggcg cggtgttcaa aaacggttac
     6661 gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta gcgcgctctc taccgatttc
     6721 cgtaacagcg ttgacgtgat gaccacggaa accacctgtt taagctccgt ctggcaaact
     6781 gatgaagaag tgcataactg gctggcgctg cacggtcgcg gccaggatta ctgccagctt
     6841 aaccctcaac cgatggcgta ctacgatggc tgcatcagcg ttgatttaag cgccatcaaa
     6901 ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac actgaaccag
     6961 aacctgaccg acattctgcg tgagattgaa attgagtccg aacgcgtggc gcacggtaaa
     7021 gccaaactct cgctgctgga taaagtggaa aatggtcgcc tgaaagtgca gcaggggatt
     7081 atcgcgggct gttctggcgg taactacgaa aacgtcatcg cggcggcgaa tgcactgcgc
     7141 ggtcaatcct gtggcaatga caccttctcg ctggcggttt acccgtcatc acagccggtg
     7201 tttatggatc tcgccaaaaa aggtgtggta gcagatttga ttggcgcagg cgcaatcatc
     7261 agaaccgcat tctgtggccc atgctttggc gcgggcgata cgccaatcaa caacggtttg
     7321 agtattcgcc acaccacgcg taacttcccg aaccgcgaag gctctaagcc agccaatggg
     7381 cagatgtcag cggtggcgtt gatggacgct cgttctatcg ctgcgactgc ggcaaacggt
     7441 ggctatttaa cctctgccag cgaacttgat tgctgggaca acgtgccgga gtacgccttc
     7501 gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg tgaaaggggc aactcagcaa
     7561 ccgctgattt acggaccgaa cattaaagac tggccggaat tgggtgcgct gactgacaat
     7621 atcgtcctga aagtgtgctc gaagatcctc gacgaagtca ccaccaccga cgaactgatt
     7681 ccttccggtg aaacctcttc ttatcgttca aatccgattg gtctggcggg gaatgtcagc
     7741 gagctgacag aggtgtttgc gcgcattaag cagattgctg gtcaggagca tattgatccg
     7801 ctgcaaactg aaattggcag catggtctat gcggtgaaac caggcgatgg ttctgcgcgt
     7861 gaacaggcgg cgagctgcca gcgtgtgatt ggcggtctgg cgaatattgc cgaggagtac
     7921 gcgactaaac gctaccgttc taacgtcatc aactggggga tgttaccgct gcagatggcg
     7981 gaagtgccaa cctttgaagt gggggattac atttacatcc ctggcattaa agcggcgctg
     8041 gataatccgg gtacgacgtt taaaggttat gtgatccatg aagatgcgcc ggtaacggaa
     8101 attacgcttt acatggaaag cctgacggct gaagagcgcg agattatcaa ggcgggtagt
     8161 ttgattaact tcaataaaaa ccgtcagatg taaaaagcgc catgtgaatg taggtcgcat
     8221 tcggcactta ttgtcggatg cgatgctggc gcatcttatc cgacctacaa atcgcatcga
     8281 accgtaggcc ggataaggcg tttacgccgc atccggcaaa tagttaatag ctcttacttc
     8341 ttcgcctctg caaccacttt gctgcccacg ccgcggttat tgtattccca catgcggttg
     8401 tagttagtgt cattcagatt gcgctgtatt tcgtcgttat catctacgct gccggtatta
     8461 cccgcaaacg gacgattaga gatcaccgca tcggcccacg gtttagccgt gttaaaacct
     8521 tcgttgatgg cgctatcacg gatcaccacc tgaccgttgg tattagcatc aacatccagc
     8581 gagcggccca gttgcgccac gccatcaccg gaagcattga aacggctgtt tacggcgagg
     8641 aaaccgtaat agatgttaga cagcgtagcc ggtgcaaaca catatgcttc ttgctgggta
     8701 cgtgagttca ccacgcggaa ttcggtgtta tcgaacacca ctgcgccgcg accagaaacg
     8761 atatccacat ccccttcaat atagctgttg gtcaccagcg tacgcggctg acggttggtt
     8821 tccagacggt tctgcacacc gctgttggtg acaaagaagg tgttctgacg accgagaatg
     8881 ttaacgttgt taatctgtac ttggtcaccg tcagtacgca gtgctaccgc cggatggtta
     8941 cctgcatcta cgctatcgcc cagcgtgttt tcgatggtca gattttgcag ttgcagtcca
     9001 ttgttttgtg accagaagac cgcagaacac agaacaccga tactgtcgct gcgtttgctc
     9061 tggcagctat cgtacatata ccacgctggt ttacctggca tatatttgcc gcgcgggttg
     9121 acgtcgtgac gccagtcggc agggctcatg cccccatcaa gggaaagccc aatcttcaca
     9181 tcaatcggtt tttcacctgt accgtacaga gtgattccac ctggagcagc agggacatac
     9241 accgttccct gatactcacc aggcatcacg gcaatatact ggcgcttgtt ggtgcgcttg
     9301 ataattgccg catctaccgc cgcctgaatc gtggtatgcg ttacaccttg agtacccgcc
     9361 gggccgacaa caaagtcagg ttgcgcaggc agggttatcg gggacggatt ccacgctgca
     9421 gcacctggtg tcagggatgc aaaatagtgt tgcgcatcga aattcagcgc ttcttttgcc
     9481 gacagaatcg ggcgagaaga ggtaccaggc gcagtttgat cagaaggacg ttgatcgggt
     9541 ggtgttgagc tacaggcggt cagcgtcacg ccaaaagcca atgccagcgc cagacgggaa
     9601 actgaaaatg tgttcacagg ttgctccggg ctatgaaata gaaaaatgaa tccgttgaag
     9661 cctgcttttt tatactaagt tggcattata aaaaagcatt gcttatcaat ttgttgcaac
     9721 gaacaggtca ttatcagtca aaataaaatc attatttgat ttcaattttg tcccactccc
     9781 tgcctctgtc atcacgatac tgtgatgc
//
LOCUS       DAOFMX010000125         9761 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.125, whole genome shotgun sequence.
ACCESSION   DAOFMX010000125 DAOFMX010000000
VERSION     DAOFMX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.125"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            383..1501
                     /locus_tag="QBA20_003767"
     CDS             383..1501
                     /locus_tag="QBA20_003767"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDQ6294136.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="QBA20_003768"
     CDS             1567..1815
                     /locus_tag="QBA20_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDQ6294137.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="QBA20_003769"
     CDS             1880..2248
                     /locus_tag="QBA20_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDQ6294138.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="QBA20_003770"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="QBA20_003770"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDQ6294139.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="QBA20_003771"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="QBA20_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDQ6294140.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="QBA20_003772"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="QBA20_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDQ6294141.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="QBA20_003773"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="QBA20_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDQ6294142.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHHHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVVRTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="QBA20_003774"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="QBA20_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2390 a         2705 c         2366 g         2300 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgccgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctgatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgct
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcacc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgatgg tgctgccact cttccagccg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gagtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg c
//
LOCUS       DAOFMX010000126         9721 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.127, whole genome shotgun sequence.
ACCESSION   DAOFMX010000126 DAOFMX010000000
VERSION     DAOFMX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.127"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(133..453)
                     /gene="psiF"
                     /locus_tag="QBA20_003775"
     CDS             complement(133..453)
                     /gene="psiF"
                     /locus_tag="QBA20_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDQ6294143.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(572..1987)
                     /gene="phoA"
                     /locus_tag="QBA20_003776"
     CDS             complement(572..1987)
                     /gene="phoA"
                     /locus_tag="QBA20_003776"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDQ6294144.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2088..2348)
                     /gene="iraP"
                     /locus_tag="QBA20_003777"
     CDS             complement(2088..2348)
                     /gene="iraP"
                     /locus_tag="QBA20_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDQ6294145.1"
                     /translation="MKNIIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2530..2733)
                     /locus_tag="QBA20_003778"
     CDS             complement(2530..2733)
                     /locus_tag="QBA20_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294146.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2811..3905
                     /gene="ddlA"
                     /locus_tag="QBA20_003779"
     CDS             2811..3905
                     /gene="ddlA"
                     /locus_tag="QBA20_003779"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDQ6294147.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVRDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3929..4141)
                     /locus_tag="QBA20_003780"
     CDS             complement(3929..4141)
                     /locus_tag="QBA20_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDQ6294148.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4401..4709
                     /locus_tag="QBA20_003781"
     CDS             4401..4709
                     /locus_tag="QBA20_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDQ6294149.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4768..5862)
                     /gene="yaiW"
                     /locus_tag="QBA20_003782"
     CDS             complement(4768..5862)
                     /gene="yaiW"
                     /locus_tag="QBA20_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDQ6294150.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5875..7095)
                     /gene="sbmA"
                     /locus_tag="QBA20_003783"
     CDS             complement(5875..7095)
                     /gene="sbmA"
                     /locus_tag="QBA20_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDQ6294151.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7447..8604
                     /gene="ampH"
                     /locus_tag="QBA20_003784"
     CDS             7447..8604
                     /gene="ampH"
                     /locus_tag="QBA20_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDQ6294152.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8605..9108)
                     /locus_tag="QBA20_003785"
     CDS             complement(8605..9108)
                     /locus_tag="QBA20_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6294153.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9196..>9721)
                     /locus_tag="QBA20_003786"
     CDS             complement(9196..>9721)
                     /locus_tag="QBA20_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ6294154.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2420 a         2483 c         2314 g         2504 t
ORIGIN      
        1 ggaaaggctg ggccacatcc ctccggcaga agcagaaaaa gcttattatg cttccatcgg
       61 aaacgatgat ctggcagcct gagttcacag ataaaacact ctccaggaaa cccagggcgg
      121 ttcaatcagg tatcaggcgg ctttcttgag gcaggcactc ataaacttat tacgatcatc
      181 acctttcaga gcttgttgcg ttgcttgatt attacattca cgcatctttt gctgctgtgg
      241 cgtcaaactt ttttcaccag gcgcagactt gctgttcttc aggcaatcac tcatgtaggt
      301 cttacgagca tcccctttca acgcctgcgc cgtcgcctgc tgattacagg aggtcatacg
      361 ctgttgttgt ggggttaagg ttctctcggc agcgccgacg gtggttaaaa aaaccagacc
      421 aaaaagcaag gtaaccagta atgttatttt catagcacca tccctcttca tgttttaacc
      481 atgagcgttt gcgcccgtga tctgccatta agtctggttg ctaacagcaa aaaaaccacc
      541 cggcagcgaa aattcactgc cgggcgcagt tttatttcag ccccagagcg gctttcatgg
      601 tgtagaagag atcggtctgg tcggtcagtc caacaacatt ggcggcatgc gggccatacg
      661 ccgcaatacg caactgactg ccggtatgtt cttgtgaatc ctcttcggaa ttcccgtaac
      721 tcatcaccat cactgcgcca tctttagtat ttagcgcctg agtgaggccc ggcgctttgg
      781 tatccggcgc aacaacctgg ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt
      841 taccctcctt tttagcgaat tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct
      901 cgccaatttg cccacaaggg ttcgcagcgt gatcctgttt atcgattgac gcaccttcaa
      961 cttgcaggaa aaatcctttc tcatttttac tcaacaattc aatggctttg tcggtcatct
     1021 gtgccagggt tggtacactg tcattacgtt gcggattagg cgtataggtg actgcgggct
     1081 tatcgatatt gccgtggtac gttgctttcg gccctagcca gcgcactggc atattgccgt
     1141 cagcaaacag gccaagcagg ggtttttgct gattcgcttc cgtcaccgaa ttcagtgagg
     1201 cagcatcgtt caccaactga taaccacgcg cctgtgcctg ttcacgcagc gtttttccct
     1261 gccattcacc agcggttgcc gtttcagcaa aggtttttgc gccgccgcca agcgtaacgt
     1321 cggcacgagc gttaagcagc tgttcggtaa tcgatccttt tccgcctttt tccagagcgt
     1381 tacccggaca tttttcactg gtcgcgctcg gaccgtagca tttgcgcgaa gtcacatgtg
     1441 ccaccagcgc agcgggcgtg gcatcctgca actctgcggt agaaacgtta ccggtcgcca
     1501 gacctgcggc ttttgccatt tccagaatcg ttgggtgatc tttttcgtga atatcgacgc
     1561 ccagcgcgcc gttataggtt ttgacaccgg ttgaccaggc ggttgctgat gcagccgagt
     1621 cggtgacgta gtccggtttg ccagtttttt tattcagcgc atagtgagtg tattgcccgg
     1681 taagcggtaa ggcatcgata cctttaaaaa agccgcccgc accttcggca taattacgtg
     1741 cggcagtaat ttccgaatcc cccatcccat cgccaatcag caaaataata ttttttgcag
     1801 gtttatcgct aagagaatca cgcagagcgg cggtctgatc acccgttaag cggcgagcac
     1861 cgccgggtgc agtaatatcg ccctgagcag cccggttttc cagaacaggc atttctggtg
     1921 tccgggcttt tgtcacaggg gtaaacagta acggtaagag tgccagtgca atagtgcttt
     1981 gtttcacttt attttctcca tgtacaaata cattaaaaaa taaaaacaaa gcgactataa
     2041 gtctcggccg tgacaacttt atgacagctg ttgaaaagat taacttttta ctgacaagga
     2101 tgcttcaata acttctttac gtaatcgcgc agcagctctg tatcgtcgtc aggaatgctg
     2161 gcatcgggct ttacctcgta cagcgccccc tctacctgat caatcaaccg ctgttggtca
     2221 ttttgcgcca tattgcgaag cattgcagtg acgataatct ccaaagcttc tacctgcgca
     2281 cacagttctt tcgactcttc ttctttttgg gcaagcttaa ataacaactc agcaatgata
     2341 tttttcatgt ctgtatttcc ttatccaaag tatggagaag ttaacattat gtttcacaac
     2401 ttcatagtgt ctggaagtat tatttttatg acaaaggaaa tactttgcgc aaatattttt
     2461 tgtttgatta ccagcgaaac gtttctctta tttaaataat tacccacaca aaatataggc
     2521 ttttaattat caacctttat tataaataaa agctatgaaa tatattctct tcgttaactt
     2581 ataattaaga gaaaaaaact caccgcattg ccatttaagc caggaataaa ttagcttccc
     2641 gttcatcatt tttgtgatct tgcgcacatc tttgagataa cgcactttct tcaccagtac
     2701 gttgcacgct ttgtcagcag aagattgcaa caatctttca gaaggccgcg aatcccccgt
     2761 tttgtcgtgg taatgtatgc actttgtgtt agacgggata ggtttttaag atggaaaaac
     2821 tgcgggtagg aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg
     2881 caaaaaacat tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg
     2941 ataaacaagg gcaatggcac gtccgcgatg ccagcaatta tctgctaaat gcagacgatc
     3001 ctgcccatat tgcgttgcgc ccttcggcga ccagccttgc gcaggtgcca ggtaaacatg
     3061 agcatcagct tatcgacgcg caaaacggtc agccgttgcc gacggtggat gtcattttcc
     3121 cgattgtcca cggtacgctg ggcgaagatg gttccttgca gggaatgctg cgggtcgcca
     3181 atttaccgtt tgtaggttct gatgttctgg cttcagcagc ctgtatggat aaagatgtca
     3241 ccaaacgtct gctacgcgat gccgggctga acattgcgcc atttattacc ctgacgcgcg
     3301 ctaatcgtca caacatcagt tttgccgaag tggagtctaa actggggtta ccactgtttg
     3361 taaaaccggc taatcagggc tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt
     3421 acgcaattgc cgtcgatctg gcgttcgagt tcgatcataa agtgatcgtt gagcaaggga
     3481 tcaaaggtcg tgagatcgaa tgcgcagttc tgggcaacga caatccgcaa gccagcacct
     3541 gtggcgagat cgtactcacc agcgatttct atgcctacga caccaagtac attgacgaag
     3601 atggcgcgaa agtggtagtt ccggcagcca ttgcaccaga gatcaacgat aagatccggg
     3661 cgattgccgt tcaggcttat cagacgttgg gatgcgcagg catggcgcgt gtagacgtgt
     3721 ttttaacccc agagaacgaa gtggtgatca acgagatcaa cacactgcct ggcttcacca
     3781 acatcagtat gtatccgaag ctgtggcaag ccagcggtct gggttacacc gatctgatca
     3841 cacgtttgat tgagctggcg ctggagcgtc acgctgcgga taacgcattg aaaaccacaa
     3901 tgtaatattt taacgcccga taatattctt attgggcgtt ttcagtttct tcatcgcgac
     3961 gacgaataat caaccccgcc agccagaaac taatcaccca cgtcaccaga ccgacggcat
     4021 aggtctgcca accttttgct tcaaatccca gtacccccac cacgccatta agaatgaata
     4081 taaggccaat ggcgaacgca tagtagtgcc agtcacggcg gatttttaca ggcagattta
     4141 tggcagctcc gttaacgaaa aacaacatcc ttgcatattc tgtaacaacc gtctgtggtg
     4201 tagacaacat tttcgtaatt attaataaat ttaacttaag gttgccattt tgtgactcac
     4261 aacgaagatt cacgcttcgg gaatattccc aacatcaatt taccatgatt ctgatattga
     4321 caatctaaat gacctgtcag acttgccata agccgctggt atcacaccag aagagaagca
     4381 gatgacttcc ggaggttttt atggctgatt tcaccctgtc aaaatcgctg tttagcggaa
     4441 aatatcgcaa tgcctcttca acgcctggca acattgccta tgcgttgttt gtgctgtttt
     4501 gcttttgggc tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc
     4561 gtttaatgca ggtccaggaa acaggtcgcc cacgggtgga aattggttta ggtgtcggca
     4621 ccattttcgg gctgatcccg tttttagcag gctgcctcat ttttgcagtg gtggcgctat
     4681 ggctgcactg gcgacatcgc cgccagtaaa atttacgttg ccagatgcgg cctgaaacgc
     4741 cttatctggc ctgacattcc tgtttgatca ctgtttcata caacgcgccc gccgttcgtc
     4801 tacgcgcttc gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc tttccagttg
     4861 aatgccaggc aacatttcgc ggggtagtga tttacccgtt ttcgcttcgg caagttgata
     4921 aactttcttg tacagcgccg tctcctcaaa agagaagctg tcacctttct ctaactgacg
     4981 gcgaatttcg ctgtcgctca tccccagttt tcccgccagt ttgcgcgttg ccagttcggt
     5041 tttcccgggt tctttactgt cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc
     5101 gctggcctta ctgactgcgt tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt
     5161 aaaatcagca aagcggtata tcggtgcgct atagctggcg ggataattca gtaaatggta
     5221 agtaccaaac cacaacccgc cgcggcgact gaagacttcc tgacgaactg taccgtccat
     5281 tttccacgga taccctttgg tatgctgttc ggcaaaagca atgctgacct gcatcggccc
     5341 accggtgcgc accggattaa gcgaaccaaa cagcgtctgc cccattggta ccatgctgat
     5401 cagatcgtca aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta accgttcgct
     5461 ataacttttg ccgtttggcg atttgatttt tagcgcggta tgaaccagaa aagcaggaat
     5521 gtgcatccgt tcagcacgac ggtcaatctc ttgccaggcg atcttgctta agcccggaac
     5581 ggccggatct gcctgataat ttgactcctg ctgcgccacc gccagcaccg agtagacgtt
     5641 ttccagcgtc ggtgccagcc cctgactttc aaaggtggtg gcgagatctt tcgcccaggc
     5701 atcgcggtct ttcacgctcg caggcatctt ctgccgcacg acgctcgcta catctatcgc
     5761 tttttcgccc tttttcaacg gttgcggagc ctgactacta caccccgcta aaaccagaac
     5821 cgccaacaat gacagcgaag atagatgatt gacgcgcgac attacttctc ctttttagct
     5881 caaggtatgg gttacttcct gaattttgtc accatccagc tcatgttcaa agctgcgcag
     5941 acgtttgtag atagacatca actcaaccag tgtggtccat gagttaatca ggtactggaa
     6001 agcaccgcga acctgaccaa aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt
     6061 accggcaaca attgacggaa acagcaagaa caaaccgaaa acgttatcga cctgcaaata
     6121 gagaatgcgg gcgatgttga aatacatata gtgaaaatag aggcgaaaat agtttttccg
     6181 tacggcgcta aacagctcgc gtaccgtagg cggcgtcgcg cgcgtggcat cgtcttcacc
     6241 ataaaccagc tctttacggt aggcggcctc tactcgctgg tttttaaact ccagccccgg
     6301 cagtttgatc cctaccactg ccagcaatcc ggtccccatc agcgaccaga cgatagcggc
     6361 aatcaccaga ccatacggaa tgtgcccgat aatcggcagc tccggcacat gcgcggagag
     6421 cgttaccagc accggcagga aggcgatcaa cgtcatgatg gcgttgataa aactgacgcc
     6481 catattctcc agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct gtgcggcacc
     6541 ttcgatatga cgcagttgtt gccagttcgc catgtaatat tcgttcatcg ccgtacgcca
     6601 gcggaacacg tagtgactga caaagaagtt gttcagtaca ctgatcacca ccgcgatcag
     6661 cgcaatcccc agaaagacgc ccacttcgcg gtaaaattgt tcgatagtga ctttatgcgg
     6721 cgaacttagc gcggtttgaa tcagatcata gaacggcgca taccaggcgt tgacggcgac
     6781 cccgacttcc accaaaaacc aagtgacgaa gataatcagc gcagtaccga gtattgacca
     6841 gtattgccaa cggtgcgggc tgtagataaa ccagaaaaat gcaaaaagtc ctacgcaaac
     6901 aatgtagtaa gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg
     6961 gatctgcccg gaagcgccgg tgatacgtgc cacccagtca cccccaccgg cttgccagaa
     7021 gataacggca atcaatgccc aaacaaaggc cgagagaaaa aacgttcccg gctttgggaa
     7081 aaaagactta aacatatgca ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat
     7141 taaccgcatg accgctacaa agcgtcagcc aaatgaaacc gcccattgtg acccatagca
     7201 aggcgcttag ttcgccagcc tgaccataag aattgtttcg acggatttca caaaccaaag
     7261 attgtttaca taagcggatt aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg
     7321 aaaaagatcg tgtttacgcg ccgttacgtt tttaacacta ttattgcact aattcgccct
     7381 ttgcaatcca tcaatgagta gtataaatac gctcagttac cttcattcaa tctatggaca
     7441 ccaccgttga aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc attgacctcc
     7501 gtccatgcgg cgcagccgat cactgaaccg gagtttgcct ctgatattgt cgatcgttat
     7561 gccgatcata ttttttacgg cagcggtgcc acggggatgg cgctggttgt tatcgacggt
     7621 aaccagcgcg tctttcgtag ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag
     7681 ctggattcgg tcgtgcgtat tgcttccctc accaaactaa tgaccagtga aatgctggtg
     7741 aaattgctcg accaggggac cgtaaagtta aacgatccgc taagtaaata tgctccgcca
     7801 ggcgcacgcg tgccgaccta caatgggacg cccattaccc tagtgaatct ggcaacccat
     7861 accagcgccc tgccccgtga acagcccggt ggcgcggcac atcgtccggt gtttgtctgg
     7921 ccaacgcgcg agcaacgctg gaaatacctt tctacggcga agctgaaagc cgcgccaggc
     7981 agtcaggcgg cgtactctaa ccttgcgttc gacctgctgg cagatgcact ggcgaatgcg
     8041 tccggcaagc cttataccca gttgtttgaa gagcagatta cccatccatt ggggatgaaa
     8101 gacaccacct acaccccctc accggatcag tgccgccgtt taatggttgc tgagcgcggt
     8161 gccagtccgt gcaataacac gctggcggca attggcagcg gcggcgtcta ttccacgcct
     8221 ggcgatatga tgcgctggat gcagcagtat ttgtcatctg atttttatca gcgcagtaac
     8281 caggcagatc gcatgcagac gttgatttat cagcgcgcgc agtttacgaa agtgattggc
     8341 atggatgtcc ccggcaaagc ggatgcgctt ggtttaggct gggtatatat ggccccgaaa
     8401 gagggtcgtc cgaggattat tcagaagaca ggcggtggtg gcggtttcat tacctatatg
     8461 gcgatgatcc cgcagaagaa tattggcgcg tttgtggtgg tcactcgttc gccgttgacg
     8521 cgctttaaaa atatgagcga tggcatcaat gatctggtga ccgagctaag cgggaataaa
     8581 ccgttggtta tccccgcatc ctgattaata ctctgaaggc aaacggttga tagcgaccag
     8641 tttgccttta ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac
     8701 gacagaacgc gatatgcgtg tacgttgatg gatataattc atcacaccaa tacgtgatcg
     8761 caattcttca ttccagtcaa tcattgataa taatgttgcg cgggtttgtt tggctggtag
     8821 atgatatccc gtacaatttc cttctgatat taatttgtat ggtatatcgt ttttcattaa
     8881 accatcagcc agccccataa tataaggagc ctgggtaata ccgataagta cgttttcttc
     8941 tctacgcaga gagataactc cttctaaaat aacgaaagta tcttcgttat tgacatcact
     9001 ggaaaatata acttgctttt cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca
     9061 tttatcgagc ttaccaaatt cctgaagagg tttaactaca gataacattt gcgcgtcctt
     9121 tgcagtaatg cccgtcaaat ccttgacggg cattatttag attaaattac cagtatttct
     9181 tcggagtgaa gaatattacc aagtatattt aacacccacg ttcgcggacc agtcttgatc
     9241 tacgtcacca ccaccgaggt agttagcatc ggtataggcg ctgaagttct tggtgaagct
     9301 gaactgagta cccagaccaa cacgtaccgc agacccttca gtaccgttat cgatggaatc
     9361 accgttcaca tcgttatcgt tgttagagtc gtcgtagacg taagccagtt tgaagtacgg
     9421 agtcagagcc tgatcttcgc tgtaggtgaa ggtataacct gcatctacac ccagttcata
     9481 acgcatgctg tcgtaaggct gaccgtcaac tttcatgtcg ttgctcagct ggtagtcatc
     9541 accagactgg aacagaccag aaatgctgcc gtaaggagtc acgtaaccag catcacccag
     9601 tttgaagtcg taaccggttt tcaaaccgaa gccccaagcg tcggagttgg tgctaccgtc
     9661 aacgtaagta ccgttgctca tggttgcaga caggtcgttg ttgaagtgag agtagctcaa
     9721 g
//
LOCUS       DAOFMX010000127         9705 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.128, whole genome shotgun sequence.
ACCESSION   DAOFMX010000127 DAOFMX010000000
VERSION     DAOFMX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.128"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(12..705)
                     /gene="ospZ"
                     /locus_tag="QBA20_003787"
                     /pseudo
     CDS             complement(12..705)
                     /gene="ospZ"
                     /locus_tag="QBA20_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            761..913
                     /locus_tag="QBA20_003788"
                     /pseudo
     CDS             761..913
                     /locus_tag="QBA20_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2294..3241
                     /gene="icsP"
                     /locus_tag="QBA20_003789"
     CDS             2294..3241
                     /gene="icsP"
                     /locus_tag="QBA20_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HDQ6294155.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3803..4175)
                     /locus_tag="QBA20_003790"
                     /pseudo
     CDS             complement(3803..4175)
                     /locus_tag="QBA20_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4419..4538
                     /locus_tag="QBA20_003791"
                     /pseudo
     CDS             4419..4538
                     /locus_tag="QBA20_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4788..5654
                     /gene="ospB"
                     /locus_tag="QBA20_003792"
     CDS             4788..5654
                     /gene="ospB"
                     /locus_tag="QBA20_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HDQ6294156.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5983..6723
                     /gene="phoN2"
                     /locus_tag="QBA20_003793"
     CDS             5983..6723
                     /gene="phoN2"
                     /locus_tag="QBA20_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HDQ6294157.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7076..8530)
                     /gene="ospC4"
                     /locus_tag="QBA20_003794"
                     /pseudo
     CDS             complement(7076..8530)
                     /gene="ospC4"
                     /locus_tag="QBA20_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
     gene            complement(8897..9415)
                     /locus_tag="QBA20_003795"
                     /pseudo
     CDS             complement(8897..9415)
                     /locus_tag="QBA20_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9432..9705)
                     /locus_tag="QBA20_003796"
                     /pseudo
     CDS             complement(9432..9705)
                     /locus_tag="QBA20_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2967 a         1833 c         1879 g         3026 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 gggagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgccatactt ctggttatac cagagatata cttcttgtca tatatgtttg gtgattcatt
      601 aaagtaacca tgttcaactc taggataaat atttctcact atatattcag tatttgcagt
      661 atttattgga aatacgttct taatattctt gatgggacta atcattttaa tctctatact
      721 caaatataaa cattaccatg aacaatataa ttgttcctaa gtggaatgtc tccacggaga
      781 acactttatc tatcgggaaa tagtgcgggc aacagtgttt aattatatcg agtgcaatta
      841 caatcgctgg cgacggcacc gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca
      901 gaacctcgct taggcctgtg tccatattac gtgggtaggc tcacagagcc tcacttacga
      961 ctgcgtccat attatgtggg taggatcaat aaagacgttt tatatgtgtc tgcttgcagc
     1021 cagaaaattt ataccctgca ggtatcataa acatgcttga taacttaatt ggggctccct
     1081 cttcctttta tggtgtaatt gtttgattga atacttccgg ggatttcagt atgaaatgaa
     1141 gtatatttaa tatactttac aaaaagtttg tattagtgga ataaggacaa tagcttatcc
     1201 aaactgccaa gcctctttat tataagtaag atctggcact ggaatattct caaaaggact
     1261 aacatcatca ccacgacctt caaccaacca ttttattgca tttaaatccc agaatagttc
     1321 agtagtggtc aatttgggaa acgcacaata ttgtacggtt acgttatcgc aatagagtga
     1381 actctcgccc tatacggcgt tttcaaggat tagtccttaa tcggacaacc acattaaagc
     1441 ataataaata attaagcttt aataatatac tatagataca gttctattat aaaaaatact
     1501 attttaggtg atgcattaaa ttagtagaat atttattctt ttataaaagc accaatgaat
     1561 tgtccaagta aacatgagat aaaatgtgta gaggataact gagtttggtc actttaactg
     1621 tattagtcgc agtctgactc tcgactttaa aaggatggga attacctgga ctgatagtga
     1681 gaaagctttc cattgaaata agtcaaataa aagttgatgg cttcagtgct aaagtgaggt
     1741 gcccgctagc cgtattcact cgaatcggaa ttcaatgtat tgcttctgcc atttccataa
     1801 tatttcacgg gaaaaacaaa atctgaaaaa ctatacttaa gtgttgtatt gagtcagttg
     1861 agatacaaat acatatttat cagcacacgt tacgtcaaat acaggaggtt gtcatgttgg
     1921 tactgaaagg cacgttggct attgcagccg tgatgacaat ttattgtctt atcgttgttc
     1981 ttatggaacg cttttctgat tgatttcata ttggcgagag aacaagaagg tgagtagaat
     2041 tgtcgcggaa tcctgaaact atcagcctca ggtatgaaca ccaaaaggta ggtagagaaa
     2101 tctatggccc ccgttacttt attcgcacaa tgaggtattt ttttattttt acaaaagttc
     2161 aataaatatt ataataaaag atatgttctt ggtaacataa acaaatattt aattaaaata
     2221 atgcttggca ttgacgttta tcttataaag taagaagatc atggacattt caaccaaaaa
     2281 agtagagttc tcgatgaaat taaaattctt tgtacttgca ctttgtgtac ctgcgatctt
     2341 tactacacat gctaccacta actatccact ttttataccg gacaacatca gtacggatat
     2401 tagtctagga tctctgagtg gcaaaacaaa agaacgcgtt tatcatccca aggaaggggg
     2461 gagaaaaatt agtcaactgg actggaaata cagtaatgcg actattgtta gaggtggcat
     2521 cgattggaag ctaattccaa aagtgtcttt cggagtttcc ggttggacta ctttaggtaa
     2581 ccagaaagca agcatggttg ataaagactg gaacaattcc aatacccctc aggtatggac
     2641 cgaccagagc tggcatccca atacgcatct ccgtgatgct aacgaattcg agctgaatct
     2701 taaaggttgg ttattaaata atttggatta tcgacttgga ctaatagcag gttaccaaga
     2761 gagtcgttac agttttaatg caatgggagg gagttatatt tatagtgaaa acggggggag
     2821 caggaataaa aaaggggcac atcctagtgg tgaaagaaca ataggttaca aacagctctt
     2881 caaaatacct tatattggat taactgctaa ttaccgccat gagaattttg agtttggagc
     2941 agaactgaaa tatagtggtt gggttctttc atctgataca gataaacact atcagactga
     3001 gacaattttt aaagatgaaa taaaaaacca aaattactgc tctgttgctg cgaatattgg
     3061 atactatgtc acccccagtg caaaatttta tatagaaggc tccagaaatt acatttctaa
     3121 taaaaaaggt gatacatctc tttatgagca aagtaccaat atatctggca ccattaaaaa
     3181 tagtgcaagt attgaatata ttggttttct cacttccgca ggtataaagt atattttttg
     3241 aattggttca ccgagttgat tacgttttca tggctaaaaa ttgccgccca ttgtgagtca
     3301 tattcgccct gactttccag aaccatacga actgcccgtt gacggacttc gggggaaaaa
     3361 cgtgtatttt tagtcattct gtttacctct ttctcagggc gtttagtctc caggatttcc
     3421 ggggcggttc aaaaaactct tcgttgatta ctgtggactt actgttggca ttaccgaccc
     3481 tgagaccgga gaaataagaa ccgcttagat catcgtggct gttctcgggg tatcaagtta
     3541 cacatgggca gaggctaccc aaggtctact attatcgcct gaaagcgctg atggaacagt
     3601 gctatcaggg acatgctgat gaaaatacct aaataatcag ccagcactct atctttccaa
     3661 atccacagca tagcaaagag agcaaaagag cctgtaaatt cagaaattaa ggaaacgcct
     3721 cttggtgtac ataatctatc ttatgttaaa taaaatctaa tttcttgatt atatgctgga
     3781 tcgttcgtgg gtggctttgc ccctatattt ccagacacct gttatcattg gcccgctgcc
     3841 gcagatattc ccgtggcgag cgataatcca gcgcactatg cggatgccat tcgttataat
     3901 gctcgaatgc ctctgcaagg ttctttgctg ccgttaaccc gtcaggtttg ggcatgatac
     3961 tgatgtagtc atactttatt gttttcacga agctctttgc tattctgtta ctctccggac
     4021 tccgcaccgc cgtgttcttc ggttcaacaa agtcccaaca tccgggtgaa ctggcgtgtt
     4081 tcattagctc ggtagcatga accattatcc gtcagtcact tcactggaga cgccggaagc
     4141 tcgttgccga agcggcgttc caccgctccc agcatgtcgc caacgggaga gcatctacgg
     4201 tctgattgaa ctgtttattc aaattgtcca ccgtctgtca gtacgctctg aacgtcggct
     4261 ggttaaaaca ctgctcgctg attttcagag ggttcacggt aaaacagcac ttttattccg
     4321 gattgcggaa gcggctctga ataatccgga tgggctggtt aaagaggttg tgtaccatct
     4381 gcatattgtt gaaccagatc gcagcggtaa gagaagttca tcatacttgg cgcagttacg
     4441 cgatgtttct gctagaggtg atgccgtaaa gaacggcaga acattaccgg aacaagatag
     4501 cggtctacct gcactggtat cagacccggg gcttccccgg atgatctcca cggtacttta
     4561 ggtactgtgt ctatattatg taggtaggat caattaactt tgtagttgaa attctttcat
     4621 tgaaataatg aataaaaaag acaaaccgca ttaaacaatg ccagaatata attgcatttt
     4681 tgttccgttt ttttaaatca tagtatacaa agaagcttgc atactatatg aaaatataac
     4741 accctttact atgatgttta taaaacaata tatggagtca tgtaggtatg aatttagatg
     4801 gtgttagacc atactgtaga atagtcaata aaaagaatga aagcatatca gatattgcat
     4861 ttgcacatat aataaaaagg gtaaaaaatt catcatgtac tcacccaaaa gcagcattgg
     4921 tttttttagg agagaaaggt ttttgtgata gcaatgatgt tctatctatt atgggacaac
     4981 aaataccaag agtatttaag aacaagatgt tatatgatta tgtttttaaa aatgaaaaaa
     5041 gtaaaaatga ttttctaaaa atggctgaat catggctacc acagagtgaa ccaatagtaa
     5101 taaataatga tgatgacgca ttgaatgctg ctgcttattt ttctgtaaaa aaagcgaaaa
     5161 taaaaacagt aaacgatact gattttaaag agtataataa ggtttatatt cttgggcacg
     5221 gtagtcctgg ttctcatcaa ttaggccttg gttcggaact tattgatgta caaacaatca
     5281 tttcaagaat gaaagactgt ggtattctaa atgtgaaaga tatccgtttt acttcatgcg
     5341 gctccgctga taaagtggct cctaaaaatt ttaacaatgc ccctgctgaa agtctttctt
     5401 gtatccttaa ctctctgcct ttttttaagg aaaaagaatc tttgctagag cagataaaaa
     5461 aacaccttga aaacgatgag tcattgagtg atggtctaaa aatatccggc tatcatggat
     5521 atggagttca ctatggtcaa gagctttttc cctactcaca ttatcgttca acttcaattc
     5581 ctgctgatcc ggagcataca gtaaaaagaa gctctcagaa aaagactttt attattaata
     5641 aagaactgga ttagtataaa atttttaacc tatagttaat tgtttgcatt taagatgaag
     5701 ttattttatc tttagtagaa aagtgcttcc ctatgaggga aaatcacaaa tcatcataat
     5761 acagagacaa aacgatacga aaaaatagat aaaaaacatc attcttttac cacattattt
     5821 tccgtgaata tgaaaaataa tgttattact ttaatataag actatttttt gtttttccat
     5881 cactctgttc aaatttttcc gcatgacttg tgttttttgt aatacagctc gttttttaca
     5941 gctgaccaaa atcatcaatt aattatgcta aggaaataaa ttatgaaaac caaaaacttt
     6001 cttctttttt gtattgctac aaatatgatt tttatcccct cagcaaatgc tctgaaggca
     6061 gaaggttttc tcactcaaca aacttcacca gacagtttgt caatacttcc gccgcctccg
     6121 gcagaggatt cagtagtatt tcaggctgac aaagctcatt atgaattcgg ccgctcgctc
     6181 cgggatgcta atcgtgtacg tctcgctagc gaagatgcat actacgagaa ttttggtctt
     6241 gcattttcag atgcttatgg catggatatt tcaagggaaa ataccccaat cttatatcag
     6301 ttgttaacac aagtactaca ggatagccat gattacgccg tgcgtaacgc caaagaatat
     6361 tataaaagag ttcgtccatt cgttatttat aaagacgcaa cctgtacacc tgataaagat
     6421 gagaaaatgg ctatcactgg ctcttatccc tctggtcatg catcctttgg ttgggcagta
     6481 gcactgatac ttgcggagat taatcctcaa cgtaaagcgg aaatacttcg acgtggatat
     6541 gagtttggag aaagtcgggt catctgcggt gcgcattggc aaagcgatgt agaggctggg
     6601 cgtttaatgg gagcatcggt tgttgcagta cttcataata cacctgaatt taccaaaagc
     6661 cttagcgaag ccaaaaaaga gtttgaagaa ttaaatactc ctaccaatga actgacccca
     6721 taaagctgga cagcctgtat caggctatgg agggcccata gacaaatcta ccctatatga
     6781 gcataggagg agtctatggg cacaccacgt tttacccctg aatttaaggg attactggaa
     6841 aggctgggac atatccctcc ggcagaagca gaaaaagctt attatgctgc catcggaaac
     6901 gatgatctgg caacctgagt tcacagataa aacattctct aggaaactcg gggcggttcc
     6961 gttcaccaca tgcaatgtgg tgttgcaggg gaacggtctg caccatcccc tatgtcgatc
     7021 aatataacag aaatgacaac ttcagattca gggcacaacc taaatatatt ttaggtcacc
     7081 tctcaaatcg tttgcctgat accgctccat tttttaataa aaaaatcaat catttttgaa
     7141 tctttgcttt tcattgcatt gtcaagcata gtgtccccac tatttgcttt ttgaaatttt
     7201 ttgtttacat caaccaatcc attattgata aaatattcta gcactttata gttggcacca
     7261 tctttactaa gtaagtattc aacatcatac agattacaat atctattgtt tttcattttc
     7321 tccatctctg cgacatcttg ttttgtaaaa ttaaatttag atagtaaata aagtgctata
     7381 tcagcttttt tattgcctaa agaataagca agggcctgat gagccatttt tttatcagtc
     7441 actttattgt taatctcttc ataattagat gctttaatcg catcctccaa agatatgtct
     7501 ctgagtttta tttttttgaa gttatctgta cttagcattc ttggtatatg atactcaagc
     7561 tgaaacacaa agtttataat tctatccaga ctaacaggat caatattttt gttataacaa
     7621 aactctctga atccttgatc tttactcttt cttataaaat caataagatg caacccaatt
     7681 ccaagcctca tatctttagt gttaaatatt ggaacatcag ttttcccctg attaccatgc
     7741 acatacctgc taacttcatc cacaacctct ttaaagttat ctaagaaaaa tgattggtgc
     7801 tcatggtaaa aaacaggtgg aatagcatta tcaaagtgat cggttaatgt catatatcca
     7861 tatggagagt catgatcaat gatatacgca ttggcgccaa aatctacagt atgatgtgtt
     7921 ttatttagtg ggagtttttc ttgatgattg gaaatttcga ccccaaaaaa aacaaaatcg
     7981 tggttagaga tttctttaat atcctcagag tatgtattct tatgtgtttg tatattttca
     8041 gactgaattt tatcaaagga tttaatatta agaatattat ttgacagatt agagttactt
     8101 tgatgtctga agttaaaatt agaattaata ataatagaaa gtaagtgacg ttcgtctgaa
     8161 gaaagaggat gaactttttg tattatctca gagatatagt gcctggacac cttatcgaga
     8221 tgctcaagtg accgaaatga tgaccgtgct ccagatggcg cttcaataga aagatttagt
     8281 gatttgaaac tggtcccttg agaaaacatt ttgctataac tttgcgctgc tatagacttc
     8341 cttaaaaagt ttttcacagt attagcacag tgatctaaat gtttttcact tatgccagaa
     8401 gaagatgagt ttgttacgcg taatatattt tttgtaatgc ttttgttgct ttattcggat
     8461 tcgtttttcc atcaacaaga tcaatattat tctgaacatt aatatgattt actgcttcag
     8521 gtattttcat aaaatattcc ccattatgtg cgtttttatc tatggttatt tagaatatat
     8581 tatctaactt aatattttct tgtgtgtatt ataacgaaat gaacatattt taatatgttg
     8641 tacaattttt actctgaaat cccctgaaat taatcacacc tcctttactg aaaggaatta
     8701 ttgcaacggt attatttgtt tttgtatata tatcttgcgc cttgttatgt agttaatcct
     8761 cagaatatga tagataaaat agagacacag agcagagcgt gttctaacat catgataaca
     8821 taaaataatt agtaacaata aatgaaagaa tcatgggagc tcaacaaaaa gagagctaaa
     8881 tagctgtgtg gaatagtaga tcactttggc ggtataatag cagatggtct gcatactttc
     8941 cggtaacaac atgggccaga agccccgctc cggcataact gcgtgcaatg ggttttgaag
     9001 gcaccggtgc ctgcacgata tggtcgcacc ggcgacaggc cagtttaggg cgttgttttt
     9061 cgataacctt aaaggcgctg ctgataagct ccagctgctc tgacacatca catcccagtg
     9121 gactgagttc accaccacag gccggacagc attcctcttc cggccggatg acccgggggg
     9181 cacggggaag tgaggctggt aacggtttac gggctgaaga ctggcgcagg gaagatggca
     9241 gtaccgggtc atattgctca cccagtgttt ccgccatttc ctcctgaagt gcgctgattc
     9301 gctcctgtgc ttcctgtatc caccgttcgg tttttgcgcg aagtttttct gagcttttac
     9361 cgaactgcat acgctgcagt ttcgcgacca gcgccttcag tcggttgatt tcggaaggcc
     9421 tggacaactg aatctgcgac ccagacctga ttaggtgctg tgggcgtaaa gttctgattc
     9481 agcagattcg gcgtaaccgg cagattgtgg tcagagtttg tggtcgccct gaacttgcgt
     9541 ttctgcttac agtgcagcct cagctcctta cgaagacgtg ccagtctgtc acgaccgacg
     9601 ataattccgt tatctgccag ctccgtctgg agccgccggg ttccatatgt ttcgcgagtg
     9661 cggatataca ccaggtgagg tgtactggca atagcggaca ctacc
//
LOCUS       DAOFMX010000128         9645 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.129, whole genome shotgun sequence.
ACCESSION   DAOFMX010000128 DAOFMX010000000
VERSION     DAOFMX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.129"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="QBA20_003797"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="QBA20_003797"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDQ6294158.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="QBA20_003798"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="QBA20_003798"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDQ6294159.1"
                     /translation="MQTFQADLAIVGAGGSGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="QBA20_003799"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="QBA20_003799"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDQ6294160.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="QBA20_003800"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="QBA20_003800"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDQ6294161.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="QBA20_003801"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="QBA20_003801"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDQ6294162.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="QBA20_003802"
     CDS             5060..6193
                     /locus_tag="QBA20_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDQ6294163.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="QBA20_003803"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="QBA20_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDQ6294164.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="QBA20_003804"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="QBA20_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDQ6294165.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="QBA20_003805"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="QBA20_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDQ6294166.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="QBA20_003806"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="QBA20_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDQ6294167.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="QBA20_003807"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="QBA20_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDQ6294168.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="QBA20_003808"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="QBA20_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDQ6294169.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2450 a         2472 c         2459 g         2264 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc tcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta
     9421 cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     9481 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat
     9541 taaactgaga aatttaatct ttgcacgttg cataaccttt aattattcat atgcattgct
     9601 gttgttactt tttatatagc taacaccaat aattaacctt tcttt
//
LOCUS       DAOFMX010000129         9499 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.130, whole genome shotgun sequence.
ACCESSION   DAOFMX010000129 DAOFMX010000000
VERSION     DAOFMX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.130"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..190)
                     /locus_tag="QBA20_003809"
     CDS             complement(<1..190)
                     /locus_tag="QBA20_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HDQ6294170.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="QBA20_003810"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="QBA20_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDQ6294171.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="QBA20_003811"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="QBA20_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDQ6294172.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="QBA20_003812"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="QBA20_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDQ6294173.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="QBA20_003813"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="QBA20_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="QBA20_003814"
     CDS             complement(3262..3381)
                     /locus_tag="QBA20_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDQ6294174.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="QBA20_003815"
     CDS             complement(3342..3527)
                     /locus_tag="QBA20_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294175.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="QBA20_003816"
     CDS             3628..3801
                     /locus_tag="QBA20_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294176.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="QBA20_003817"
     CDS             3803..4147
                     /locus_tag="QBA20_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDQ6294177.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="QBA20_003818"
     CDS             4151..4486
                     /locus_tag="QBA20_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDQ6294178.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="QBA20_003819"
     CDS             complement(4543..6864)
                     /locus_tag="QBA20_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDQ6294179.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="QBA20_003820"
     CDS             complement(6981..7190)
                     /locus_tag="QBA20_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294180.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="QBA20_003821"
     CDS             complement(7941..9464)
                     /locus_tag="QBA20_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDQ6294181.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2664 a         2204 c         2079 g         2552 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccagtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccagcca
     9481 aagggtttaa tacatgaaa
//
LOCUS       DAOFMX010000130         9442 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.131, whole genome shotgun sequence.
ACCESSION   DAOFMX010000130 DAOFMX010000000
VERSION     DAOFMX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.131"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            100..252
                     /gene="hokE"
                     /locus_tag="QBA20_003822"
     CDS             100..252
                     /gene="hokE"
                     /locus_tag="QBA20_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDQ6294182.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            603..755
                     /gene="hokE"
                     /locus_tag="QBA20_003823"
     CDS             603..755
                     /gene="hokE"
                     /locus_tag="QBA20_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDQ6294183.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(877..1497)
                     /gene="entD"
                     /locus_tag="QBA20_003824"
     CDS             complement(877..1497)
                     /gene="entD"
                     /locus_tag="QBA20_003824"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDQ6294184.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1672..3912)
                     /gene="fepA"
                     /locus_tag="QBA20_003825"
     CDS             complement(1672..3912)
                     /gene="fepA"
                     /locus_tag="QBA20_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDQ6294185.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKNENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4155..5357
                     /gene="fes"
                     /locus_tag="QBA20_003826"
     CDS             4155..5357
                     /gene="fes"
                     /locus_tag="QBA20_003826"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDQ6294186.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5360..5578
                     /gene="ybdZ"
                     /locus_tag="QBA20_003827"
     CDS             5360..5578
                     /gene="ybdZ"
                     /locus_tag="QBA20_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDQ6294187.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5575..9420
                     /gene="entF"
                     /locus_tag="QBA20_003828"
     CDS             5575..9420
                     /gene="entF"
                     /locus_tag="QBA20_003828"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDQ6294188.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLSGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2057 a         2484 c         2603 g         2298 t
ORIGIN      
        1 taactgtcca actttttggg gtcagtacac cctggggctg ccactccaaa ccagacaatt
       61 tggatggtag tcccttcttc gcatggaggc aatataaaca tgctgacgaa atatgccctt
      121 gtggcagtca tagtgctgtg tttgacggta ctgggattta cgcttctggc cggagactcg
      181 ctgtgtgagt ttacggtgaa ggaacgtaat attgagttca gggctgttct cgcttacgaa
      241 ccgaagaagt agccgttgtg cggggagtaa tccccgcata tccggttgtc aggtcaggat
      301 ggtaaggcac ctgctttaca ctttcgcccg cggtcagtga tggctgcggg cgatttcgtt
      361 ttaagagcaa tgatgagttt tgccgacaga agtccgaaac aggactcact gtataaataa
      421 acagctattt tgctaaggaa gggtaagata acggcgggtg cctgaagctt tccggtttca
      481 ggttaactct gaggtttgga aagatgaagc cccaagagat atttctatca atcctggggc
      541 tgccactccg aacctcaaca actcggatgg tagtcccttc ttcgcatgga ggcaatataa
      601 acatgctgac gaaatatgcc cttgtggcag tcatagtgct gtgtttgacg gtgctgggat
      661 ttacgcttct ggtcggagac tcgctgtgtg agttcaccgt aaaggaacgt aatattgagt
      721 ttaaggctgt tcttgcttac gaaccgaaga agtagccgtt gtgcggggag taatccccgc
      781 atatccggtt gtcaggtcag agtggtaagg cacctgcttt acactttcgc ccgcagtcag
      841 tgatggctgc gggcgaatcg taccagatgt tgtcaattaa tcgtgttggc acagagttat
      901 gactattttt tcttttatct gccagtgcac agcaaacatc tcattctcac gatgaatgat
      961 gacctgctgt ttattccagc taattatctg atagtccaga aaacctgcat cagtttggat
     1021 ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgca agtgtcagcg ccagagaaaa
     1081 ggctaaaccg cagtctgcga gtcgctcgtg ttctgctggt gtaataatgt tgtctgtcaa
     1141 ttcggtcgct gtttgcgcag aaaaaatttc ttcgatatca acgccaatcg gttgacggga
     1201 aaccacggcc aatgccgtag ccccgcagtg gctaatactg ccgtatacct ccgcaggcca
     1261 caccggttgg cgtagctcgc cgattgcggg cacacattta tagccatatt cccgcaaggc
     1321 ataaacagca gcgatccgtc cggctaaatg ctctgtttta cgtttacgtc cagcgtgttg
     1381 cagttgtgcg tagtgcggca gccagagtaa atcctgctca caaaaattcg ccggatcgaa
     1441 ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag gtatgcgtag ttttcatatc
     1501 gaccatattc gagactgatg acaaacgcaa aactgcctga tgcgctacgc ttatcaggcc
     1561 tacgtgatcc tgcaatatat tgaattggca agatctttgt aggccggata aggcgttcac
     1621 gccgcatccg gcgtgaacga cgcgcacttt gtcaacaatc tgacgttagc atcagaagtg
     1681 agtattaatg ctcatatacc acgtacgtcc cggctcgtta taggtatacg cgccggcacc
     1741 ggcgatatag ttggctcctg ccagatcgcc cgtggtctgg gcattacctg cacgccacaa
     1801 acgtttgtcg aacaggttgt ccacgccgcc ggtcagactg acattcttcg tcacatccca
     1861 ggtcgcgctc agacccacaa tgctgtaagg actgatttct ttggtttccg ggccaaccgc
     1921 tggctgacct ttatagttgt acttcttcgg ctgctgcttg ccgtaccagg tgaaggtcgt
     1981 ttgcatcgac aaatcttccc gtgcctgcca gctcagcgtt gagttcaacg tgtactccgg
     2041 gatgatcgac aaacggtcgc ccgtggtttt gttttcattc ttcagcatat aagtgatgtt
     2101 attggtccac atcaccgttt cgctaactgg tacgtttaac gatccttcca gaccttcaac
     2161 cactgctttc ggcacgttat cccactgata gagatcagtg ccgactgcgt tttgccctac
     2221 agccacatag cccgcttcaa tcttattgcg ataatcgtta cggaaccagg tgacgcccgc
     2281 cagccagccg tcgcgtttga actccagacc aatctctttg ttgatgctgg tttctacttt
     2341 caggtcatcg ttaccttgca gatagcagcc gcccgcgctg gcatagcagc cctgaccttt
     2401 actgtagaga atgtagttcg ggttagtctg gtataggctc ggcgctttat aggcgcgggc
     2461 aatgcctatt ttcagcgtga agtcatcgcc taaaccttgc gatatgttca gcgccgggct
     2521 ccagttattg ccgacaatac tgtgatgatc gaaacgcagc cccggcgtta cgatggtgct
     2581 gtcagtcagc tccatgttgt tttcggcaaa cagcgagaaa atttctgctt ttgaatacgg
     2641 gctacggtcg gtggcactca cgccatcaat agcgccaccg gtattcgttc cggtcagtgc
     2701 ttgggtgttg gaacttaagt ccttcatccg ttgctgattc cactccgtac ccagcgtcag
     2761 cgtctggtta acgaggaaat caatcggcag gttaacttcg ctgtgcagca tcacgtcatc
     2821 gagatcgata tcgacgaaat cctgtgtcgc tttttcgtta aatttccctt cggtaccgcc
     2881 cgccagacct tccggaatac gcgagttacg ggtgtgttca tactgcaccc agttgctggt
     2941 ggtcacgccg ttatcccagc caccgttcca ggtcagcgcg tagttctggc gatacaggcg
     3001 gttggtttca tcgccatatt tcgagcgagt gtaagcgtca gagttggtgt tctgggtatc
     3061 gcccgcatac aggttaccct ggcggctgta acccgcttcc agttccagcg attgcaatgg
     3121 tgcgaagtcc cagcgcacca cgccgttaat atctttattg atcacccctt cgcgcccggc
     3181 tggtaacgtc gtggcatacg ttccggcacg cgcggactga tggccctggt tgatatccca
     3241 cgcgtcagcc tgagttttgt cgaggttgcc atacaagcgg aagctgaatt cgtcgcccag
     3301 cggaccggtc aggctaaagt tagtgcgttt ggtggcacct tcctctttat gttctggcgc
     3361 attgaaatat gcgtcccagg agccgtgcca ctcgccgctg ccttttttgg taatgatgtt
     3421 aaccacgccg cccgccgcgc cgttgccata acgcgcagct gccggaccac gcagaacttc
     3481 aatacgttca atcatttcag gtggcaccca ggaggtatca ccacgggtat cgcgctcgcc
     3541 acgccagccc tgacgcaccg agttacggct gcttaccggc ttgccgtcaa tcaaaatcag
     3601 cgtgttttcc ggacccatac cgcgaatatc aatctgtcgg ttattcccac gctgaccact
     3661 ggtggagtta ccggtcaggt taacgcctgg catggtacgg atgatctccg acacatcgcg
     3721 ggcaaccggg tttttgcgga tttcatctgc ggtgatggtc gaaacgccag gagcctgcaa
     3781 gttctgctcg gcggcggtaa cgacaatggt atcgtcatgt gaaacaggtg tatcggtcgg
     3841 ctcttgtgcc tgcgctaccc cataaatccc cagattgacc aacaaggcca gggaatgaat
     3901 cttcttgttc attgttttat tcctgcattt ttgccacgaa ttgcaactgt cgggcatggt
     3961 cgtcatcaac acgacgcatc ccgttaccgc gaaaaccttt gatcctgaaa gacacgcagt
     4021 gcagttggtt aattaatgtc cgcgcttccc acggcgcgcc attacgctat tgcaaatgca
     4081 aatagttatt aataacatta tcaatatatt tctgcaatca atgaaaaatt gcacagtaaa
     4141 catggggtta tggtgtgacg gcgttaaaag taggaagtga gagctggtgg cagtcgaaac
     4201 atggcccgga atggcagcgt ctgaatgacg aaatgtttga ggtcactttc tggtggcgtg
     4261 atccccaagg ttctgaagaa tactcgacga taaagcgcgt atgggtctac atcactggtg
     4321 tgaccgatca tcatcagaac agccagcccc agtcgatgca gcgaattgca ggtactgatg
     4381 tctggcagtg gacgacacaa ctcaatgcca actggcgcgg cagctactgc tttattccca
     4441 ccgaacgcga tgacattttt tctgcaccat cccccgatcg cctcgaatta cgtgaaggct
     4501 ggcgaaaact attaccccag gcaatagccg atccgctgaa tccacaaagc tggaaaggcg
     4561 ggctagggca cgctgtttct gcactcgaaa tgccgcaagc gcctctgcaa ccgggatggg
     4621 attgtccgca agcgccagaa ataccagcca aagaaattat ctggaaaagt gaacggttga
     4681 aaaattcacg tcgtgtatgg atttttacca ccggcgatgt aacagcagaa gaacgcccgc
     4741 tggcagtttt gctcgatggc gaattttggg cgcaaagtat gcccgtctgg cctgtgctga
     4801 cttcgctgac ccatcgtcag caacttcctc ccgccgtgta tgtgttgatc gacgctatcg
     4861 acaccacgca ccgcgcccac gaactgccgt gtaatgcgga tttctggctg gcagtacagc
     4921 aagagttatt acccctggtg aaagttattg ccccttttag cgatcgcgcc gatcgcaccg
     4981 tggtcgccgg gcagagtttt ggtgggctgt ccgcgctgta tgccggactg cactggcctg
     5041 aacgctttgg ctgtgtatta agccagtcag gatcgtactg gtggccgcat cggggcgggc
     5101 agcaagaggg cgtgttactt gaaaagctaa aagctggtga agttagcgcc gaaggtctgc
     5161 gcattgtgct ggaagcgggt attcgcgagc cgatgatcat gcgggccaat caggcgctgt
     5221 atgcgcaatt acaccctata aaagaatcca ttttctggcg tcaggttgac ggcggacatg
     5281 atgcgctttg ttggcgcggt ggcttgatgc aggggttaat cgacctctgg caaccacttt
     5341 tccatgacag gagttgaata tggcattcag taatcccttc gatgatccac agggagcgtt
     5401 ttacattttg cgcaatgcgc aggggcaatt cagtctgtgg ccgcaacaat gcgccttacc
     5461 ggcaggctgg gacattgtgt gtcagccgca gtcacaagag tcctgccagc agtggcttga
     5521 agcccactgg cgtactctga caccggcgaa tttcacccag ttgcaggagg cacaatgagc
     5581 cagcatttac ctttggtcgc cgcacagccc ggcatctgga tggcagaaaa actgtcggat
     5641 ttaccctccg cctggagcgt ggcgcattac gttgagttaa ccggagaggt tgatgcgcca
     5701 ttactggccc gcgcggtggt tgccggatta gcgcaagcag atactttgcg gatgcgtttt
     5761 acggaagata acggcgaagt ctggcaatgg gtcgatgatg cgctgatatt cgaactgcca
     5821 gaaattatcg acctgcgaac caatattgat ccgcacggta ctgcgcaggc attaatgctg
     5881 gcggatttgc aacaagattt gcgcgtcgat agcggtaaac cactggtctt tcatcaactg
     5941 atacaggtgg cagataaccg ctggtactgg tatcagcgtt atcaccattt actggtcgat
     6001 ggcttcagtt ttccggccat tacccgccag atcgccaaca tttactgcgc attgctgcgt
     6061 ggcgaaccaa cgcctgcttc gccgtttacg cctttcgctg atgtagtgga agagtaccag
     6121 caataccgcg aaagcgaagc ctggcagcgt gatgcggcat tctgggtgga acagcgtcgc
     6181 caactgccac cgcccgcgtc actttctccg gcacctttat cggggcgcag cgcctcggca
     6241 gatattttgc gtctgaaaat ggaatttacc gacggggaat tccgccagct ggctacgcaa
     6301 ctttcaggtg tgcagcgtac cgatttagcc cttgcgctga cggctttgtg gctggggcga
     6361 ttgtgcaatc gcatggacta cgccgccgga tttatcttta tgcgtcgact gggctcggcg
     6421 gcgctgacgg ctaccggacc cgtgctcaac gttttgccgc tgggtattca tattgcggcg
     6481 caagaaacgc tgccggaact ggcaacccga ctggcaaccc gactggcagc acaactgaaa
     6541 aaaatgcgtc gtcatcaacg ttacgatgcc gaacaaattg tccgtgacag cgggcgagcg
     6601 gcaggtgatg aaccgctgtt tggtccggta ctcaatatta aggtatttga ttaccaactg
     6661 gatattcctg gtgttcaggc gcaaacccat accctggcaa ccggtccggt taatgacctt
     6721 gaactggccc tgttcccgga tgaacacggt gatttgagta ttgagatcct cgccaataaa
     6781 cagcgttacg atgagccaac gttaatccag catgctgaac gcctgaaaat gctgattgcc
     6841 cagttcgccg cggatccggc gctgttgtgc ggcgatgtcg atattatgct gccaggtgag
     6901 tatgcgcagc tggcgcagat caacgccact caggttgaga ttccagaaac cacgctgagc
     6961 gcgctggtgg cagaacaagc ggcaaaaaca ccggatgctc cggcgctggc agatgcgcgt
     7021 tacctgttca gctatcggga aatgcacgag caggtggtgg cactggcgaa tctgctgcgt
     7081 gagcgcggcg ttaaaccagg ggacagcgtg gcggtggcac taccgcgctc ggtctttttg
     7141 accctggcac tccatgcaat agttgaagct ggcgcggcct ggctaccgct ggataccggc
     7201 tatccggacg atcgcctgaa aatgatgctg gaagatgcgc gtccgtcgct gttaattacc
     7261 accgacgatc aactgccgcg ctttagcgat gttcccaatt taaccaacct ttgctataac
     7321 gccccgctta ccccccaggg cagtgcgccg ctgcaacttt cacaaccgca ccacacggct
     7381 tatatcatct ttacctctgg ctccaccggc aggccgaaag gggtaatggt cgggcagacg
     7441 gctatcgtca accgcctgtt gtggatgcaa aatcattatc cgctcacagg cgaagatgtc
     7501 gttgcccaaa aaacgccgtg cagttttgat gtctcggtgt gggagttttt ctggccgttt
     7561 attgccgggg ctaaactggt gatggctgaa ccggaagcgc accgcgaccc gctcgctatg
     7621 cagcaattct ttgccgaata tggcgtaacg accacgcact ttgtgccgtc gatgctggca
     7681 gcatttgttg cctcgctgac tccgcaaacc gctcgccaga gttgcgtgac gttgaaacag
     7741 gttttctgta gtggtgaggc cttaccggct gatttatgcc gcgaatggca acagttaacc
     7801 ggcgcgccgt tgcataacct gtatggcccg acggaagcgg cggtagatgt cagctggtat
     7861 ccggcttttg gcgaggaact ggcacaggtg cgcggcagca gtgtgccgat tggttatccg
     7921 gtatggaata cgggtctgcg tattcttgat gcgatgatgc atccggtgcc gccgggtgtg
     7981 gcgggtgatc tctatctcac cggtattcaa ctggcgcagg ggtatcttgg acgacccgat
     8041 ctgaccgcca gccgctttat tgccgatcct tttgctcctg gtgaacggat gtatcgtacc
     8101 ggagacgttg cccgctggct ggataacggc gcggtggagt acctcgggcg cagtgacgat
     8161 cagctaaaaa ttcgcgggca gcgtatcgaa ctgggcgaaa tcgatcgcgt gatgcaggcg
     8221 ctgccggatg tcaaacaagc cgttacccac gcctgtgtga ttaaccaggc ggcagccact
     8281 ggtggtgatg cgcgtcagtt ggtgggctat ctggtatcgc aatcgggcct gccgttggat
     8341 accagcgcat tgcaggcgca gcttcgcgaa acattgccgc cgcatatggt gccagtcgtt
     8401 ctgctgcaac tgccgcagtt gccacttagc gccaacggca agctggatcg caaagcctta
     8461 ccgttgcctg aactgaaggc acaagcgcca gggcgtgcgc cgaaagcggg cagtgaaacg
     8521 attatcgccg cggcattcgc gtcgttgctg ggttgtgacg tgcaggatgc cgatgctgat
     8581 ttctttgcgc tgggcggtca ttcgctactg gcaatgaaac tggcagcgca gttaagtcga
     8641 cagtttgctc gtcaggtgac gccggggcag gtgatggtcg cgtcaaccgt cgccaaactg
     8701 gcaacgatta ttgatggtga agaggacagc tcccggcgca tgggattcga aaccattctg
     8761 ccgttgcgtg aaggtaatgg cccgacgctg ttttgtttcc atccggcgtc cggttttgcc
     8821 tggcagttta gcgtgctctc gcgttatctc gatccacaat ggtcgattat tggcattcag
     8881 tcgccgcgcc ctcatggacc catgcagaca gcgacaaacc tggatgaagt ctgcgaagcg
     8941 catctggcaa cgctacttga gcaacaaccg cacggcattg cggcgcggct gcgtgcccgt
     9001 ggcgaacagg tggcatttct tggtttgctg gatacctggc cgccagagac gcaaaactgg
     9061 caggaaaaag aggctaatgg tctggacccg gaagtgctgg cggagattaa ccgcgaacgc
     9121 gaggccttcc tggcggcaca gcagggaagt acttcaacgg agttgtttac caccattgaa
     9181 ggcaactacg ctgatgctgt gcgcctgctg acgactgctc atagcgtacc gtttgatggt
     9241 aaagcgacgc tgtttgttgc tgaacgcacg cttcaggaag gtatgagccc cgaacgcgcc
     9301 tggtcgccgt ggattgcgga gctggatatc tatcgtcagg attgtgcgca tgtggatatt
     9361 atctctccag gggcgtttgt aaaaattggg ccgattattc gcgcaacgct aaacaggtaa
     9421 attaatatta tctataaacc ca
//
LOCUS       DAOFMX010000131         9295 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.132, whole genome shotgun sequence.
ACCESSION   DAOFMX010000131 DAOFMX010000000
VERSION     DAOFMX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.132"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="QBA20_003829"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="QBA20_003829"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDQ6294189.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..3047
                     /locus_tag="QBA20_003830"
     CDS             1647..3047
                     /locus_tag="QBA20_003830"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDQ6294190.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            3417..4241
                     /gene="gadX"
                     /locus_tag="QBA20_003831"
     CDS             3417..4241
                     /gene="gadX"
                     /locus_tag="QBA20_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDQ6294191.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            4609..5337
                     /gene="gadW"
                     /locus_tag="QBA20_003832"
     CDS             4609..5337
                     /gene="gadW"
                     /locus_tag="QBA20_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDQ6294192.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(5700..8813)
                     /gene="mdtF"
                     /locus_tag="QBA20_003833"
                     /pseudo
     CDS             complement(5700..8813)
                     /gene="mdtF"
                     /locus_tag="QBA20_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(8838..9095)
                     /locus_tag="QBA20_003834"
                     /pseudo
     CDS             complement(8838..9095)
                     /locus_tag="QBA20_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         2431 a         2162 c         2278 g         2424 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
     2101 cggataaacc aaacctagtg tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct
     2161 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga
     2221 aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga
     2281 cctacaccgg taactatgag ttcccacaac cgctgcacga tgcgctggat aaattccagg
     2341 ccgacaccgg tatcgacatc gacatgcaca tcgacgctgc cagcggtggc ttcctggcac
     2401 cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg
     2461 cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg
     2521 aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctgggtggt caaattggta
     2581 cttttgccat caacttctcc cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc
     2641 tgcgcctcgg tcgtgaaggc tataccaaag tacagaacgc ctcttaccag gttgccgctt
     2701 atctggcgga tgaaatcgcc aaacaggggc cgtatgagtt catctgtacg ggtcgcccgg
     2761 acgaaggcat cccggcggtt tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc
     2821 tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc ttcactctcg
     2881 gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg
     2941 actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc agcgatcacc
     3001 cgaaactgca gggtattgcc cagcagaaca gctttaaaca cacctgataa cataacgttg
     3061 taaaaaccga atgcccagcc tttaaaaaaa cagctgggca ttcgtttgct tattaatcgc
     3121 aataatatat tggctgtcta ttcatcgtgt tgataagata tatgttatat gaatgtttat
     3181 taaatgtagc gattaccttt aatgtcacga aaagggatta tttgcttgct attaatttca
     3241 ctgcgttctt aaacacgttt ttgtattata aatccagtca tcctgcccgt agaacatcac
     3301 acattatcat cctgttctcc cgcttcgttt aaatttattt atcaatcaat ttgacttaag
     3361 agggcggcgt gctacattaa ttaacagtaa tatgtttatg taatattaag tcaactatgc
     3421 aatcattaca tgggaattgt ctaattgcgt acgcaagaca taaatatatt ctcaccatgg
     3481 ttaatggtga atatcgctat tttaatggcg gtgacctggt ttttgcggat gcaagccaaa
     3541 ttcgagtaga taagtgtgtt gaaaattttg tattagtatc aagggatacg ctttcattat
     3601 ttctgccgat gctcaaggag gaggcattaa atcttcatgc acataaaaaa atttcttcat
     3661 tactcgttca tcactgtagc agagatattc ctgtttttca ggaagttgcg caactatcgc
     3721 agaataagaa tcttcgctat gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt
     3781 tatctgtttt tcttgaggat gagcacttta taccgctgct tttgaacgtt ttgcaaccga
     3841 acatgcgaac acgagtttgt acggttatca ataataatat cgcccatgag tggacactag
     3901 cccgaatcgc cagcgagctg ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag
     3961 aagagacatc atattcacag ttgcttactg agtgtagaat gcaacgtgct ttgcaactta
     4021 ttgttataca tggcttttca attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt
     4081 cgtatttcat ttacgtcttt cgaaattatt atgggatgac gcccacagag tatcaggagc
     4141 gatcggcgca gggattgccg aaccgtgact cggcggcaag tattgttgcg caagggaatt
     4201 tttacggcac taaccgttct gcggaaggaa taagattata gagttttact cagacataaa
     4261 aaaaacccgg cataggggac cgggaagagg atagtctgcc gtctccagac taataaaccg
     4321 ttataacact ccctgttggc acgggaaact ttgtgctctc agtaagttaa atataacttt
     4381 tactggaaat aagatcagcc atttttttat aaacataagc tatacgctgt gcgaaaatat
     4441 attcgtgctg catttactta ttatcaatta actgttatgc aaaactactt tgtggataaa
     4501 ttttggtcct accaaatctg gcagtttttg cgctaagaaa cagtctgtca tcatttcatt
     4561 agtatactga aattgaaata atcgcagtat gaaatataag ggataatcat gactcatgtc
     4621 tgctcggtga tcctcattcg tcgttcattc gatatttatc atgaacagca aaaaatatcg
     4681 ctgcataacg agagtatcct gctgctggag aaaaatttgg cagacgattt tgcgttttgt
     4741 tcaccggata cgcgacgact ggatatcgat gagctgacag tttgccatta cttacaaaat
     4801 attcgtcagc taccacgcaa tttagggtta catagcaaag accgtctgtt aattaaccag
     4861 tcacccccca tgccgctggt gacggcgatt tttgatagct tcaatgaatc cggggtaaat
     4921 tcaccgatac tgagcaatat gctctacctt tcctgtttat cgatgttttc tcataagaaa
     4981 gaactgatcc ccttactttt caatagcatc agcactgttt caggaaaagt tgaacgcctt
     5041 attagctttg atatcgccaa acgttggtat ctgcgcgata tcgcggaaag aatgtatacc
     5101 agcgagagtc tcatcaaaaa aaagttgcag gatgaaaata cctgtttcag taaaatatta
     5161 ctcgcttcca ggatgtcgat ggccagacga ttactcgagt tacgtcaaat tcctctgcat
     5221 actattgcgg aaaaatgtgg ctatagcagt acgtcgtact ttataaacac atttcgacaa
     5281 tattatggtg taacgccaca tcagtttgcg caacattcgc caggtacctt ttcctgacat
     5341 attttgcatt tgaatattgg tcaggatctc acacctgctt catgtgaaac tcttccctga
     5401 tgatttctgc cgggctaccg gctagttctc tttcgcagtt tgccaggcgg tataggtatt
     5461 tttaggagga aaagactcgg catgtttggg attattaagc tgacaattca tacgattacg
     5521 ggaatgtggg tcagtattgt gttgttcaaa ttgatgacga atggctggag tggtttctac
     5581 tttcagtgct gtgtactaag cctggtattc ctgacggtta gctggttgtt gagtggtgaa
     5641 tggttagcag gaaagagtaa ggctgaacct tcacgttcaa ccttactctc atttacacgt
     5701 tacgcttttt taaagcgggc aaagagatgt tccactacaa cgaaaaagac cggaacgaag
     5761 taaattgcca gcactgttgc ggcaaacatc ccgcccatca cgccggtacc taccgcgttt
     5821 tgcgcgccag aaccggcacc atgactgata accagcggca gcacgccgag aataaaggcc
     5881 agagaggtca tcaggattgg gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc
     5941 ggcgttttcc cttctttctg catcatctca acggcaaatt cgacaatcag gatggcgttc
     6001 ttggcggaaa gcccgatggt ggtcagcaaa ccaacctgga agtagacgtc attacttaag
     6061 ccgcgcagat cggtggccag taatgcgcca acgacgccta acggaacaac caacatcacc
     6121 gagaacggaa ttgaccagct ctcatagagt gcggcgaggg cgaggaacac cacgaccagt
     6181 gaaatggcat acagtgcagg agcctggttt gaggataacg cttcctgata cgacagtccg
     6241 gtccatgagt agccgacgcc tgccggaagt ttagcgacca ggtctgccat aaatttcata
     6301 gcgtcaccgg tacttttccc ggccgccgct tcacctaaaa tctccattga cgggatgccg
     6361 ttgtagcgtt ccaatcgcgg tgaaccatag gtccattcgg tagacgagta ggcagaaagc
     6421 ggtgccatcg tgccagaggc gttgcgtaca taccattggt tgatgttatc cggcaacata
     6481 cggaacggcg tgcctgcctg gacatacact tttttcaccc gcccctggtt gaggaagtcg
     6541 ttcacgtagc tgctgccgaa ggcggtggaa attgtctggt tgatatcaga cagcgccaca
     6601 cccattgcct cagctttcgc ggcgttgacg ttcactttga acatcggcgt atcttccagg
     6661 ccgttcgggc gtaccccggt gacctggtca ggcgattgcg ctgccagtga taacagctcg
     6721 tttcgtgcct aggttagttt ttcgtgcccc aggttaccgt tgtccagcag ttccatatca
     6781 aaacctgacg cggtacccag ttcagccacc gcgggtaagt tgaacgggaa gacgacggct
     6841 ttattgatac tgcttaacgc aatcattgcc cgctgaatga tcgcggtaac cgagttttcc
     6901 tcaccgacac gttcagacca cggcttgaga ctgataaacg ccaggccgtt gttttgcccc
     6961 tggccgctga agccaaagcc gccaacggta aacaccgact gaacattatc tttctctttt
     7021 gtaagataat aatccgtcac ctgttgcagt actttcgtgg tgttaaccat ggtggcaccg
     7081 gaaggtaact gcgcggtggt cataaatacc ccctgatcct cttctggtaa gaaagaggtc
     7141 ggtgtacgca ggaacagcac cgccatcccg gcgcaaatca gcaggtagac caccatgtag
     7201 cgaccggtac aacgcaacag cgagcgggtg ctatcggtat agtgctgagt tgatttttca
     7261 aacagcgtgt tgaagcgtgc gaacagggcg ttaggtttgt gaccgccttc cggcgtggct
     7321 ttcagaatgg tggcgcacag ggcaggggtc aggctcattg ccacaaatac tgaaagccgc
     7381 atggaggaga tcagcgtgat ggagaactgg cggtagatct ccccggttgc accgctcata
     7441 aaggccatcg gcataaacac tgcagaaaga acaacggcaa tcccgaccag cgcacgttgg
     7501 atctgcccca tcgatttatg cgtcgcttcc ttcggcggta gcttatcttc cgcaatgaca
     7561 cgctcgacgt tctccaccac cacgatggcg tcatccacca gtaaccctat cgccagcacc
     7621 atcccgaaca tagtcaacgt gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc
     7681 ccgagaataa ccaccggtac ggcaattgtc gggatgatgg tggcacggaa attctgcaaa
     7741 aacagataaa tgaccaggaa gacgaggatg atagcctcaa ccagtgtttt gaaaacttcc
     7801 tgaatagaaa tttcgataaa cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg
     7861 gggaaatagg ctgataagcg gttcagctcc tctttgactg cccgcgaggt atccagggcg
     7921 tttgctccgg cagccagttt gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc
     7981 acggtggaat aatcttccgc cccaagttcg acgcgggcga catcacgcag cagcacttgc
     8041 gaaccatctt gctgaacttt caacaggatt ttgccaaatt cttccggcgt ttgcagacgc
     8101 gtctgcacaa tgatcgaggc gtttagttgc tggtctgccg cctgtggcat gccacccagt
     8161 tgaccaccag aaatctggtt gttttgcacc ttaatctggg aaataacatc ggaaggtacc
     8221 aggttatatt tattgagttt ttgcgggtcc agccagatac gcatggcgta ctcggaacca
     8281 aagagctgta cgctaccaac gcccgcggta cggcttagcg ggtctttgat attagacgct
     8341 acatagtccg cgatatcgta ctggttgagg ctgccgttat cagaaataaa cgccgctacc
     8401 atcaggatat tactgctcga cttatcgacg ctaatcccct gttgctgcac cgcttcaggt
     8461 aatgaaggca tagcgagttg cagtttattt tgcacctgaa cctgtgcgat atcaggagat
     8521 gtcccagtct cgaaggtcag agtgatagag gcattacccg ccgcatcact ggttgaagac
     8581 atgtacatca ggccatcaag cccattcata ttttgctcaa tcacctgagt gaccgagtct
     8641 tctaccgttt gcgcatcggc acccggatag gtagcgctga cggtaatggt cggtagcgca
     8701 atctgcggat actgcgcaac cggtaagttc atgatcgcca gaccacctgc aagcatcata
     8761 ataatggcaa gtacccaggc aaaaaccggg cgatcaataa aatagttagc catgaaagtc
     8821 cccttaagcc tgcaacgtta ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa
     8881 attgctcgtg ctttgatacc cggacgaatg cgttgcaaac cggaaacgat cacccgatcg
     8941 ccagcctgca agccagaggt gacaacccac tggtcgccga tggctttgct ggcttcaatt
     9001 tcgcgtagct gcacgacatc gtctttatcc agaatgagcg ccgttgcttt accctgggcg
     9061 ttgtgggtga cgccttcctg cggcaccagt aatataaagc gcgcaaaatt attcaagata
     9121 tttttgaaaa tcattcaaga taggctatcg cgtgtgatag caaaaacatt caagataaaa
     9181 atgcttgatt attaagcaat caggaatggg agtattaagt ttcgtgtttc cagtaaaacc
     9241 agagagtatt gaaatagcta tcacctaact ttatcaggcg atagctggta gatga
//
LOCUS       DAOFMX010000132         9192 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.133, whole genome shotgun sequence.
ACCESSION   DAOFMX010000132 DAOFMX010000000
VERSION     DAOFMX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.133"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..904)
                     /locus_tag="QBA20_003835"
     CDS             complement(<1..904)
                     /locus_tag="QBA20_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDQ6294193.1"
                     /translation="MNRRRKLLIPLLFCSAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNV"
     gene            964..1242
                     /locus_tag="QBA20_003836"
     CDS             964..1242
                     /locus_tag="QBA20_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294194.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="QBA20_003837"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="QBA20_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDQ6294195.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="QBA20_003838"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="QBA20_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDQ6294196.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="QBA20_003839"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="QBA20_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDQ6294197.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="QBA20_003840"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="QBA20_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDQ6294198.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="QBA20_003841"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="QBA20_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="QBA20_003842"
     CDS             complement(4922..5452)
                     /locus_tag="QBA20_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6294199.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="QBA20_003843"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="QBA20_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDQ6294200.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6421..7644)
                     /locus_tag="QBA20_003844"
     CDS             complement(6421..7644)
                     /locus_tag="QBA20_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294201.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8225..>9192)
                     /locus_tag="QBA20_003845"
     CDS             complement(8225..>9192)
                     /locus_tag="QBA20_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDQ6294202.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2683 a         1825 c         1923 g         2761 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcaacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ctgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatca gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgctggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
     6241 tgaggtgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag
     6301 ttcaattccg ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt
     6361 gttttaaaat tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata
     6421 ttatggtatt atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc
     6481 tatttccacc ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg
     6541 gtatgaaatc ttttcgggtt gaagtgtctc gtaatgaaat gctatgaatt catttttaga
     6601 cattgttgcc catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg
     6661 tttataacat atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt
     6721 cactaccttt gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa
     6781 agagttgtca ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg
     6841 ttgttcatat ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa
     6901 taaatttgcg tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt
     6961 taggactagc ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc
     7021 aagggcttcc atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa
     7081 gtacaagctt tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt
     7141 tttattatcg gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag
     7201 aattttagtt atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac
     7261 agaatcctta tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg
     7321 ttgcgtaagt ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac
     7381 cacaactttt gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg
     7441 ttggagttgt tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt
     7501 ggaattaatg ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt
     7561 tttaacatta tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat
     7621 acctggggta aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt
     7681 ttttaaattg gataattaga acaggaatgg attttcttct acacacaata aatataatgc
     7741 gttatatctt cctgatataa ttacctttaa atttcctttc atttccaccc ggtaatattt
     7801 gtgtataaaa aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa
     7861 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
     7921 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
     7981 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
     8041 taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc
     8101 cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta
     8161 tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat
     8221 ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg
     8281 tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca
     8341 ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg
     8401 tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga
     8461 caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg gcaatccacg
     8521 ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca
     8581 gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta
     8641 ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac
     8701 tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac
     8761 aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca
     8821 ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt
     8881 tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa
     8941 ataaaaaagt gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg
     9001 gtattgtcga gcaactacag aacggggtaa cggctcccaa cagagcagcc aggagatagc
     9061 ctctcccttt tctggccccc atcatctgct ggattttatg gtctggcacc ttttgtctta
     9121 tcaggctgac accggcacta atcactataa acagcaacga aagctcgacg gcgagaaaca
     9181 caaacatctc tg
//
LOCUS       DAOFMX010000133         9170 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.134, whole genome shotgun sequence.
ACCESSION   DAOFMX010000133 DAOFMX010000000
VERSION     DAOFMX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.134"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(227..2770)
                     /gene="mdoH"
                     /locus_tag="QBA20_003846"
     CDS             complement(227..2770)
                     /gene="mdoH"
                     /locus_tag="QBA20_003846"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDQ6294203.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHSTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2763..4298)
                     /gene="mdoG"
                     /locus_tag="QBA20_003847"
     CDS             complement(2763..4298)
                     /gene="mdoG"
                     /locus_tag="QBA20_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDQ6294204.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4692..5849
                     /gene="mdoC"
                     /locus_tag="QBA20_003848"
     CDS             4692..5849
                     /gene="mdoC"
                     /locus_tag="QBA20_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HDQ6294205.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5857..7338)
                     /gene="clsC"
                     /locus_tag="QBA20_003849"
     CDS             complement(5857..7338)
                     /gene="clsC"
                     /locus_tag="QBA20_003849"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDQ6294206.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEARLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7280..7813)
                     /gene="ymdB"
                     /locus_tag="QBA20_003850"
     CDS             complement(7280..7813)
                     /gene="ymdB"
                     /locus_tag="QBA20_003850"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDQ6294207.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7908..8219)
                     /locus_tag="QBA20_003851"
     CDS             complement(7908..8219)
                     /locus_tag="QBA20_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6294208.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8340..8672)
                     /gene="csgC"
                     /locus_tag="QBA20_003852"
     CDS             complement(8340..8672)
                     /gene="csgC"
                     /locus_tag="QBA20_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDQ6294209.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8731..9096)
                     /gene="csgA"
                     /locus_tag="QBA20_003853"
     CDS             complement(8731..9096)
                     /gene="csgA"
                     /locus_tag="QBA20_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDQ6294210.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT         2295 a         2368 c         2181 g         2326 t
ORIGIN      
        1 gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga gccagtcagt
       61 tgcttaactg actggcatta cggcataacg ccggtatttt tattggctcg ttgtttttgc
      121 cggatgtggc gtgaacgcct tatccgacct acaaaatctt gcaaaatcaa taaattgcag
      181 gaacgatgta ggcctgataa gcgtagcgca tcaggcaact acgtttttat tgcgaagccg
      241 catccggttt acgcaatgcc agtggattga gctttatccc ttcgtaataa ctcacccatg
      301 aagaatatct ttcaggggaa ttccagacgc ggaaatgcag acgggccatc gtcaccggat
      361 cgcttagcag caccaggcga cgatcgcgat tcagcttctc tggcgtctcg ttaagcgcct
      421 gttcaacgtg gcggtcacgg gcgatttcca gcaccttact ggcgcggtga cgcgctgtcg
      481 ccattgcggt tgccagagcg ttaaatgacg ggttaaacac tgcgtgcata aagccatcgt
      541 caagggagcg ttggcgattc atctcaagga accgatcggt atcaaccagc acctgcggcg
      601 gtgaatactc ttccgggatc aggaacagtt tccagcgttt ggtgcgcaga ccaacggtgg
      661 cacggctgga aatcaccgaa acaaacggtg acaggatcaa cgagaagaca atcggtgcca
      721 gccagaacag gaaacgcaga tccagccacg ccatcccaac agcccacact aaccccaaca
      781 gcagctgtga gccgtggcgt ttgaacgctt cgccccatga agtggagtca tcatcacgct
      841 gcggtgaatt ccacaccact tcccagccaa ggaacgcgct gacaacgaag accgtatgga
      901 acagcatgcg taccggagcc agcagcacgg aaaacagcac ttccagcagc aacgataatg
      961 taacgcgcca gaagccgccg tattctttcg ttcctttgca ccagataagc aaaatgctca
     1021 ataacttcgg caggaacaac agcaccatgg tcgaagcaaa aagtgcaatc gccagctcag
     1081 gacgccactg cggccacacc gggaacaact gccgtggttg caggaagtat tgcggttcgg
     1141 tcaacgcatg cactacctgc aatgcagtag agagcgcgag gaacataaac cacagcggag
     1201 cggagagata agacatcacg cccgtcagga acaccgcacg gtgaaccggg tgcataccct
     1261 taaccaggaa cagacggaag ttcatcaggt taccgtggca ccagcggcgg tcacgtttta
     1321 gctcatcaag caagttaggc ggcaattctt cataagaacc cgggagatcg taagcaatcc
     1381 agacccccca acctgcacgg cgcatcaacg ccgcttctac gaagtcatgt gacaggattg
     1441 aaccggcaaa ggaaccttcg cccggcagcg gagccagtgc gcagtgctcg ataaatggtt
     1501 tcacgcggat aatcgcgtta tgtccccagt agtgcgactc gccaagttgc cagaagtgca
     1561 aaccggctgt aaacagtggc ccatacacgc gggtcgcgaa ctgctgacag cgcgcataca
     1621 gcgtatccat accggacgct ttcggcgacg actgaatgat cccggcgttc gggttggctt
     1681 ccatcaagcg caccaggccg cataaacaat caccggtcat taccgagtca gcatccagca
     1741 ccaccatgta gctgtactgg ctgccccagc gacggcagaa gtcatcgata ttaccgcttt
     1801 tacgcttcac gcggcgacgg cggcggcgat agaaaatctg accttcgcca ccgacttcag
     1861 cgataagctc catccaggct ttttgctctg cgacgcagat atccgggtta taactgtcac
     1921 tcagaatgta gacatcaaag tgtttggcat tcccggtggc ttttactgat tcccacgttg
     1981 cacgcaagcc agcaaaaaca cggttcacgt cttcgttaca gataggcatg atcaacgccg
     2041 tgcgatgctc cgggtttaat ggttcatcgc caactgttga cgcagatata ctgtatttat
     2101 cgcgaccaat aagcagttgc aggaagccca tcaacgccgt ccagaatccg gcggacaccc
     2161 aacagaacag taccgcaaaa aggatcagga taccggtttg cagcatataa ggcagaagct
     2221 gcataaagga aacccacaaa tcctgaccaa ccatatccat aggattaatc agcgcccaac
     2281 cctgataagg aagaatggtc ttcatatacc aggtcgcgac aacagtttgc gcgagcgtca
     2341 ggatcaacag aatgtaacgg cggatggtac caacggtacg ccacttttgc tcgctctcct
     2401 gctcttcttt ggtcaaacga gccagatagc gcggcgtgac atcgcgtcca cgcaggcgat
     2461 cccagaaacg gcctaccggg ttggtacgcc acgggtcggg aaacatcgag gagcgttttg
     2521 cttctggcat cgccttaagc tgatcgcgcc cttcgtcgtc tttaattaac tgtccatcag
     2581 caagtgaatc tggccaggct tgttccagac gcgcctttac cgagccttgc ggggaatcat
     2641 cctcccgcgc ccaggtgctg tgttcggcat ccagcgcctg atgaacggcg cggatatcag
     2701 tcttcggcaa tgctgctttc tcgcttgcgg cgatgggcat tgcgtcaatg tactcagttg
     2761 tcttattcat tggcaggtaa ctggtagctc caggtttcac tcaacgtctg atcggcattc
     2821 accagcgcag cacgcatttc agtgattttc ttggcatctt tcactttcac acgcatcacc
     2881 agacgccagc ctttggtaac cgggttatag cgcaccgtgc tttcaactat ctcaccatta
     2941 tcaccaatgc tggtttgcgc tgtgaccggg gtatcctctg gcagtttttt catctctgcg
     3001 ccggtaaaat cgaccacaaa ggcgatagta ccgtcaggct ggcgaatcag gttcgactgc
     3061 ttcacatccc ccgttgaacg acgcgtttgt tgcacccatg cgttatctgg cgcatgcagt
     3121 ttgtcttcat cacggctgaa ggtgatggtg tatttaaagt tcatctcttt acccggctcc
     3181 ggcagctgat ccggcgtcca gtaagcgacg atgttatcgt tggtttcatc gttggttgga
     3241 atttccacca gctcaacgct gcctttgccc cactcccctt tcggagtcac ccatgcgctt
     3301 ggacggagat cgtaacgatc atcgagatct tcaaagcggg agaaatcacg acctcgttgc
     3361 aacagaccaa agcctcgcgg gttttccatg gagaagctgc tgaccgctaa atgtttcggg
     3421 ttattcaacg gacgccagat ccattcgcca ttaccagcat ggatagacag accgttagag
     3481 tcgtgcaact ccggacgata gttatttgca ggcgacggtt ggttcggccc aaacaggaac
     3541 atactggtta acggtgcaac ccccagtttg ccgactttat cgcgcagata gattttcgac
     3601 tgcacatcca caaccgtgtc acgccctggc ataaccacga atttgtaagc acctgtcgca
     3661 cgcggcgagt caagcaatgc ataaatggtt aaacgtttat cagtcggttt tggacgctcg
     3721 atccagaact ctttgaagcg tggaaattct tcacccgatg gcaaagcggt atcaattgct
     3781 aggccgcggg cagaaaggcc ataaacctga cctgcaccaa tcacgcggaa atagctggcc
     3841 ccgagcatgc tgacgatttc atcgttttta tctttgctgt tgatcgggta aagcacttta
     3901 aaaccggcaa aaccaaggtc ttttaccgtg tctttgtcat gctgaacatc gccgaaagtg
     3961 aaataatccg ggctgtattt gattcgtttg actgcggtgg cagtcacttc atttattttg
     4021 accggggtat cgaagtacat accctgatgg tagaactcga gtttgaatgg ggtcttcaga
     4081 ttgttccagt acgctttgtc atgattaaac tggatctgct gatagtccgc gtatttcatg
     4141 tcgcggaaaa cggagggcaa gttgctttta ggcgcctcat agcctttccc ggctaaggat
     4201 tgagcttgct ttgcgacatc atcaatactg aaagcccagc ttgaagatgt atacagggtt
     4261 aacattactg cagcactcaa ccaacgcatt ttcatcattt gtagtttatg tttcataatt
     4321 agtaagcact tccccctttg tgtgcttata tcgatccgat ctattttaat ggataaccag
     4381 ccattccgac aactgaatcc cggtatcgtt cagcacgctg gctcatggat taagcacctg
     4441 attgcaaccg ttttcacttt ccgtgcttct cctaaagaca gaagcatcga cttcgtgtag
     4501 ggtttgccag caaatttatt tatcaaccgt cttacagacg cttccattct ggttagtggt
     4561 agcgatgatg ctatgcataa aaaccttgaa gcctgcgcct ggcgcggtca ggtcggtgtc
     4621 gctatggcaa caagcctaaa ggcataaccc gacagggata agacgaaaaa tccgagatta
     4681 gttaaccata tatgaatcca gtacccgcgc aacgtgaata tttcctcgac tccatccgcg
     4741 cctggctgat gttgttaggg atcccttttc atatttcttt aatctattcg agccatacat
     4801 ggcatgtgaa tagcgccgaa ccgtcattgt ggctaaccct ttttaatgac ttcatccact
     4861 cgttccgcat gcaggtattt ttcgttatat ccggatactt ttcctacatg ctttttttac
     4921 gctatccctt gaaaaaatgg tggaaagtac gtgtcgaacg tgtaggtatc ccgatgttaa
     4981 cagccatccc cctactgaca ttaccgcaat ttattatgct gcaatatgtc aaagggaaag
     5041 cggaaagttg gcctgggcta tcattgtatg acaaatataa tacgttggcc tgggaattaa
     5101 tatcacacct gtggttttta ctggtgttag tagtcatgac gacgctgtgt gtatggatat
     5161 ttaagcgcat cagaaataat ttagaaaatt ctgataaaac gaataaaaaa ttctcgatgg
     5221 taaaactatc ggtgattttt ttatgcctcg gcatcggcta tgcggtaata agaagaacga
     5281 tttttattgt gtatccaccc attctgagta atggcatgtt caattttatt gtcatgcaaa
     5341 cgctatttta tttaccgttc tttatcctcg gcgcactggc tttcattttc cctcatctta
     5401 aagccttgtt taccacgccg tctcgtggct gtaccctcgc tgcagcattg gcgtttgtcg
     5461 cttatttact caaccagcgc tatggcagtg gcgatgcctg gatgtacgaa accgagtcgg
     5521 tgatcaccat ggtcctcggc ctgtggatgg tgaatgtggt cttctccttt ggccaccgtt
     5581 tgcttaactt ccagtcagcg cgggtgactt attttgttaa cgcatcgctg tttatctatc
     5641 tggttcacca cccgttaacg ctgtttttcg gcgcatacat tacaccgcac atcacctcca
     5701 actggcttgg ttttctctgt ggcctgatat tcgtagtagg gattgcgata attctgtatg
     5761 aaattcattt acgcatcccg ttactgaagt ttttgttctc tggtaaaccg gttgttaagc
     5821 gtgagaacga taaagcacca gcccgttaag ccacatttac aataaccatt ccacgggcaa
     5881 tatcgacgcc agtctgacca taacccgctt ccagaaactg gtggcgggtt cttttttgag
     5941 aataacctct ttctttgcat gacgatcaac ccagttgatc cgtccccacc tgtccagacg
     6001 gagctgccag gccgcatcgt actggctctg aataaagcgt ttatcaatta actgtgccag
     6061 cgtttcgctc tctatcacga agcccatttc agtattgagc aatgttgaac gcggatcgaa
     6121 attgaaagag ccgataaaca ccgttttacc atcgatgcta aaagttttag catgcaggct
     6181 ggcaccggaa ttaccggtta tgccgcgatc gtgtaacgta ctgctttgtt cacgcgtcgg
     6241 cttgagttca tataattcca cgccatagcg gagcaatttt ttgcgccagc gcgcgtatcc
     6301 ggcatggacg acagcaacat cgttagcggc aagagaattg gttaggatcg caatctttac
     6361 cccttttctc accatccgta agagttgcgc cacacctgcg cgtgtcggta caaaatagga
     6421 agagataata tcgatgcgtt cactgggtga gcccatgata tcgaacaggc gctgcggtaa
     6481 cagtgaatgc cgttttgcct tgccctcccc tttcgccgga tcatcactta ataaacgtgt
     6541 cttcgcccag ataagcggca acgttccatc aaccagatga tttataaatg gactggattc
     6601 cattttgcgt aaataacgat gcgtcatggc atcgttatgc caggaggcgg gtaactcgat
     6661 gcgatccgcc atttcacctt ccgggacatc cagcacctgc tgtaagggtg aaaccgattt
     6721 gcaataccag tagcgggcga aatcatcggc aacatcctct accacggggc ctattgccat
     6781 gacatctaaa tccgaaaaaa gtggctcttc ccctgcacca aaataggcat caccaatatt
     6841 tcgtcctccc actagggtaa ccacgccatc gacagtgaaa cttttattgt gcatccggcg
     6901 attgagacgg gaaaagtcgg tgatatatcc aagcggacgt agcaagcgaa acgagaaagg
     6961 attaaaaagc cgggcttcaa tgcgtggatg actgtcaagc aagcgcaaaa tgtcgtcaag
     7021 tccgggcgta ttgttgtcgt ccagcaacaa acggacgcga acgccacgct ttgctgcggc
     7081 taacagggcg gaaaacagta accgccccga catatcgtcc tgccagatgt aatactgaac
     7141 atccagcgta tgctctgcca tttcggccag acgataccgg gcggcaaacg catccagact
     7201 tttctccaga gggaaaagac cacactgacc gggatgttgc gaacacagtg gcagcaccgc
     7261 gctcgccagc cggggcaaat cattcatctc cttgttgggt aaggagtctt tcgtagaggt
     7321 gggcgttttc ttcatcataa cagacaaagt atacctgttc gggtaaagcg tgacgggtaa
     7381 taaattctga aacggtttta actgcgattt cagccgctgc cgcacgaggg taactataaa
     7441 ccccagtact gattgcagga aaagccactg acgtatagct gtttgccgcc accagtcgta
     7501 ggctattcag ataggcatcc tgcaaaagct ggtcttcgtt ttgttcccca ccacgccaga
     7561 ctggcccgac ggtgtgtact acggctttag cgggaagatc gcctgcaagc gtaataacgg
     7621 catgccccgt agggcaatcg ccctgctgtt gcctgacttt taaacaggca tccagcaggg
     7681 ccggacccgc tgcgcgatga atggccccat cgacgccgcc gcctcccatt aatgacggat
     7741 tagccgcatt cacaatcaca tcaacggcca gtttggtaat atcaccctgc acaacatgaa
     7801 tacgcgtttt cataacgcct ccttacacct tcttgtttct taagtgtaca gcaggctttt
     7861 tggttaaatc gcatttttct gctcagcaac gtgagttgtt cagaaactta gttatattcg
     7921 atgttcatta cagccagctt tttctgttta tcaagatact gcacttttac tgaggctatt
     7981 tgcgcgtttt tgacattgcc gctcattaac gctttgctgg agtaggtttt gcccggaata
     8041 gaaccattct gtgggcagtt catttctacg ggtgatgact gggtgctgac gtcacatgcc
     8101 ggttccacaa tttggccata aaaatggatc acgccccctt gcgcggtgct tcctgcaatg
     8161 gcaataaaag gaaaaaacaa actgccgagc ataagagagt ctaaaaaagg tctgaacatt
     8221 tattctattt cctcaatgat tagtcatcct tgagggttgt gttatccata ctttcaggtt
     8281 ggctgattcg cagcagacca ttctctccag attcatctta tgctcgatat ttcaacaaat
     8341 taagactttt ctgaagaggg cgtccattgt tgtgataaat gaagtgcctg tccatcagaa
     8401 acagtaacaa ctattttcac ccgatcttcc ggggaaatat ttaaactcaa cttcgtcaaa
     8461 gcaatgggtt gattagcagg caatgagagg gtcttttctt gcttcgtctg actttgccct
     8521 gaactgcctt cgcgcaggga caatatttgt actctgcaca gacaagattg agtaagagta
     8581 acttcaggaa taatggtgta catatcccct tgctgggtcg tattaaaggt tatctggctg
     8641 gaaagtgccg caaggagtaa taacgcattc atattcttct cccgaaaaaa aacagggctt
     8701 gcgccctgtt tctgtaatac aaatgatgta ttaatactga tgagcggtcg cgttgttacc
     8761 aaagccaacc tgagtcacgt tgacggagga gttcgatgca gtctggtcaa ctgcagcacc
     8821 gttgccacca ccgaactgtt taacagtcat ttcagaattt ttgccgttcc actgatcaag
     8881 agtggcgctg ttaccgaagc cacgttgggt cagatcgatt gagctgtcat ctgagccctg
     8941 accaacatct gcaccattac caccgccatg ctgggtaata gtcaagtcag agttacgggc
     9001 atcagtttgc agagcaagtg cagagttacc gccaccgtac tggtaaatgt tcagctctga
     9061 atttgggccg ctattattac tgaggtgtac tggcaatagc ggacactacc atttgttctt
     9121 ttttaagcag ccatctgatg atatttttcc ctgaaggctg ccggggagat
//
LOCUS       DAOFMX010000134         9096 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.135, whole genome shotgun sequence.
ACCESSION   DAOFMX010000134 DAOFMX010000000
VERSION     DAOFMX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.135"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="QBA20_003854"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="QBA20_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDQ6294211.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="QBA20_003855"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="QBA20_003855"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDQ6294212.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="QBA20_003856"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="QBA20_003856"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDQ6294213.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="QBA20_003857"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="QBA20_003857"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDQ6294214.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="QBA20_003858"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="QBA20_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDQ6294215.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="QBA20_003859"
     CDS             5545..6018
                     /locus_tag="QBA20_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDQ6294216.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="QBA20_003860"
     CDS             6047..6589
                     /locus_tag="QBA20_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HDQ6294217.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="QBA20_003861"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="QBA20_003861"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDQ6294218.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="QBA20_003862"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="QBA20_003862"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDQ6294219.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="QBA20_003863"
     CDS             7986..8243
                     /locus_tag="QBA20_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDQ6294220.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="QBA20_003864"
     CDS             complement(8219..8773)
                     /locus_tag="QBA20_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDQ6294221.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2282 a         2209 c         2270 g         2335 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag agaaaaaatc ctgaaattca gggttgactc tgaaagagga aagcgtaata
     9061 tacgccacct cgcgacagtg cgctaaagcg cgtcgc
//
LOCUS       DAOFMX010000135         8982 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.136, whole genome shotgun sequence.
ACCESSION   DAOFMX010000135 DAOFMX010000000
VERSION     DAOFMX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.136"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..923
                     /locus_tag="QBA20_003865"
     CDS             <1..923
                     /locus_tag="QBA20_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6294222.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="QBA20_003866"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="QBA20_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDQ6294223.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="QBA20_003867"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="QBA20_003867"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDQ6294224.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="QBA20_003868"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="QBA20_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDQ6294225.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="QBA20_003869"
     CDS             4446..4805
                     /locus_tag="QBA20_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDQ6294226.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="QBA20_003870"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="QBA20_003870"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDQ6294227.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="QBA20_003871"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="QBA20_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDQ6294228.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="QBA20_003872"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="QBA20_003872"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDQ6294229.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2039 a         2174 c         2431 g         2338 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcacc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcacc
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gcgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tc
//
LOCUS       DAOFMX010000136         8940 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.137, whole genome shotgun sequence.
ACCESSION   DAOFMX010000136 DAOFMX010000000
VERSION     DAOFMX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.137"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..2887
                     /locus_tag="QBA20_003873"
                     /pseudo
     CDS             1..2887
                     /locus_tag="QBA20_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="QBA20_003874"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="QBA20_003874"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDQ6294230.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="QBA20_003875"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="QBA20_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="QBA20_003876"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="QBA20_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="QBA20_003877"
     CDS             complement(7280..8041)
                     /locus_tag="QBA20_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDQ6294231.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="QBA20_003878"
     CDS             complement(8038..8697)
                     /locus_tag="QBA20_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDQ6294232.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2180 a         2369 c         2328 g         2063 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcac ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccagcaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt ctcttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcttg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg
     8881 agggttaggg agaggtttcc cccgttactt tattcgtacc ccttataatg gggtgttagc
//
LOCUS       DAOFMX010000137         8762 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.138, whole genome shotgun sequence.
ACCESSION   DAOFMX010000137 DAOFMX010000000
VERSION     DAOFMX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.138"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            5..436
                     /locus_tag="QBA20_003879"
     CDS             5..436
                     /locus_tag="QBA20_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDQ6294233.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="QBA20_003880"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="QBA20_003880"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDQ6294234.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="QBA20_003881"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="QBA20_003881"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDQ6294235.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="QBA20_003882"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="QBA20_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDQ6294236.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="QBA20_003883"
     CDS             complement(4438..5145)
                     /locus_tag="QBA20_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDQ6294237.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="QBA20_003884"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="QBA20_003884"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDQ6294238.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="QBA20_003885"
     CDS             complement(6003..6206)
                     /locus_tag="QBA20_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDQ6294239.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6395..7873
                     /locus_tag="QBA20_003886"
                     /pseudo
     CDS             6395..7873
                     /locus_tag="QBA20_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7870..>8762
                     /locus_tag="QBA20_003887"
     CDS             7870..>8762
                     /locus_tag="QBA20_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HDQ6294240.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT         2033 a         2389 c         2251 g         2089 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct tactcgtgag gatttatgtt ctgctggcag
     6361 tttcgtgatc aacacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt
     6421 gttgtcttgt tcattgtttt tatcgtcggc atggggatgt cggctttccg cacctgaaca
     6481 taatatttac cagaaacagg gaaaaggcgt ggtgtacctg cgcccttata aagaaacaaa
     6541 tctttctcta cctgacgtga attacaagag tttgcgtcgg ttacccaatt tattaataga
     6601 cccgacaaca ctagacgaat gggataaaga accgcctctg actgatttga caaccgacta
     6661 cctctatgag ggcgcacagg cctggtatcc tcattattca tggcatagcg atggtcgcaa
     6721 tatcctctac gcgggtgaag ttgtgcaaaa ccctcccggc acacctcccg tcgatgtcgc
     6781 ctcgttcaag gcatggggtg attttgccgc agataaacac agtctctatt ttgaaggcaa
     6841 gcgcaccgat gacaatggtg gtggaaatag cttggatatc aaaacgctgc atcaggtgga
     6901 attccggcct ccctgggatc ctgacttact ggggctgata ttacgtgacg ctaatttctt
     6961 gtatatcaat gggcatcgcc tcgctgaccc cgagagtttc cgtgtgctgg cgcagaagtc
     7021 ctgggatcag cgaggaaagt tttccaccac gtttaacccc tgcattgccg taccatttgg
     7081 cccctgggat accctggctc gtacacggac caaaatcctg ctcaacggcg aacagcttga
     7141 tgccgacccg gacacctttt ccgtcgtgcg ctggatgccc ggctcactct tgacctggcg
     7201 tgataaaaac gggctgcagc gtaaagtcct cgacaaggaa aatctggcgt gggatgaaga
     7261 tttaacaaag cactgtctgg atttttctct gctggaaaag aaagtgttct ggcgtaaagg
     7321 acctgcttgt aaacaggaag aattacccgg actcgatccg gagcagtttc aacccatcag
     7381 tgatgctgtc gcccagcatc aggactcgct ttataccatc atcgaaacag agtctggtga
     7441 ccgtaagctg gagatcgtga aacttgatga tcccaatctt attatcaaca aacgtttcaa
     7501 cgccgcgaaa cgccacggct atttacttac gcgtgccgaa gggtagccat accattccgg
     7561 tttacacgtg tttgaatctg acggaccgct gatcttactg gataaccgtt ctccggatga
     7621 acgcgaagcc catcttaatg accatccctt tttgcgcaga tggtatgccc gcgataaccg
     7681 ctacatttac agctttgatg gcgcgcagct ctggcgatac cgcaccgctg atccgaaaca
     7741 agttcgctta atctggaagg aacaacattc gggatatgtc tatggcgtaa attacaaaac
     7801 gggatatctg gacggaaaaa ttactgatga cggcgaattt attcctgccc cgcgcaatga
     7861 ggcgacaaaa tgaagggtca aataatcaca gttgcactca tcctgttggg acacacattt
     7921 ctgacgcctg ctgtacaggc catagggttc gactattaca atgatcacgg tgttatgtct
     7981 tacggtaaag gttattgggg ggataagaaa atcattgccc agtttccgga gatgagcagg
     8041 gccggtttgc gccttgtgaa aaatatctct gggaaaacgg gttatatacc ttccgatgaa
     8101 ataagcatag aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt
     8161 ggcaaaatcg ttagcaatcc accgggaaca ctcactgtcg atattgccag ctttcaggct
     8221 atgggccgct tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt
     8281 aatagcggcg cgtcacgtgt tgagctggcg acactaaaag ctatcgaagg taactctacc
     8341 acgctggtgg atagtaaaaa tctctacttg tccggtcgac gtcaggggag cagcagtgat
     8401 gttactgtat tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga
     8461 aattcatatt ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt
     8521 caccttacgg cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg
     8581 gtttttcgcg acaataaggg tctgcatcgt tatccctttg gtcaattatc aggcaaagcg
     8641 atacccgtag atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa
     8701 cagctcacgc ccgaccgtca gtggagcaag tggatagacc taccaggtat tgaacctgaa
     8761 ca
//
LOCUS       DAOFMX010000138         8636 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.139, whole genome shotgun sequence.
ACCESSION   DAOFMX010000138 DAOFMX010000000
VERSION     DAOFMX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.139"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(770..1966)
                     /gene="hemY"
                     /locus_tag="QBA20_003888"
     CDS             complement(770..1966)
                     /gene="hemY"
                     /locus_tag="QBA20_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDQ6294241.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1969..3174)
                     /gene="hemX"
                     /locus_tag="QBA20_003889"
     CDS             complement(1969..3174)
                     /gene="hemX"
                     /locus_tag="QBA20_003889"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDQ6294242.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3196..3936)
                     /gene="hemD"
                     /locus_tag="QBA20_003890"
     CDS             complement(3196..3936)
                     /gene="hemD"
                     /locus_tag="QBA20_003890"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDQ6294243.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3933..4874)
                     /gene="hemC"
                     /locus_tag="QBA20_003891"
     CDS             complement(3933..4874)
                     /gene="hemC"
                     /locus_tag="QBA20_003891"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDQ6294244.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5261..7807
                     /gene="cyaA"
                     /locus_tag="QBA20_003892"
     CDS             5261..7807
                     /gene="cyaA"
                     /locus_tag="QBA20_003892"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDQ6294245.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7847..8167)
                     /gene="cyaY"
                     /locus_tag="QBA20_003893"
     CDS             complement(7847..8167)
                     /gene="cyaY"
                     /locus_tag="QBA20_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDQ6294246.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8215..>8636)
                     /locus_tag="QBA20_003894"
     CDS             complement(8215..>8636)
                     /locus_tag="QBA20_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HDQ6294247.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1966 a         2233 c         2219 g         2218 t
ORIGIN      
        1 cagttgacgg ggtatctgct gcgctggcca tgagaggaag agcggcggcg acagcaacaa
       61 caagacgttt gggtgaaaac gaaaattcca tgcaaaatgc tccggtttca tgtcatcaaa
      121 atgatgacgt aattaagcat tgataattga gatccctctc cctgacagga tgattacata
      181 aataatagtg acaaaaataa attatttatt tatccagaaa atgaattgga aaatcaggag
      241 agcgttttca atcctacctc tggcgcagtt gatatgtaag gcaggtttat tatatcgcgt
      301 tgattattga tgctgttttt agttttaacg gcaattaata tatgtgttat taattgaatg
      361 aattttatca ttcataataa gtatgtgtag gatcaagctc aggttaaata ttcactcagg
      421 aagttattac tcaggaagca aagaggatta cagaattatc tcataacaag tgttaaggga
      481 tgttatttcc cgattctctg tggcataata aacgagtaga tgctcattcc atctcttatg
      541 ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt gcttatcgtc
      601 cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg agtgaagcac
      661 ccacttgttg tcatacagac ctgttttaac gcctgctccg taataagagc aggcgttttt
      721 ttatgcatca gaaaggtccc ggaggtgctt tcctccgggt gagaaggaac tactgtggcg
      781 ggttattctg caacgttaac atcaaaccgt cgcgacgcat cgctgcagct tcttccggct
      841 tgtgcagtct gtccagcgcg tcggcaagcc atgcgtaatc gtaggcgtcc ggacgttgtt
      901 tcagcgccgc gcggaaggcg agcgatgctt cctgccattc accgtgcttc atcagtgact
      961 gacccaatgt gctccacaac agcgggcgat caccgacgtt tttgatttgc tggcgtagca
     1021 ctttttccag ctgttccggg ttattggttt tcagacgcgg gatcggcagc agtaggcgat
     1081 cgtcatactg gcgtttcagg ccgtcgatga taatttgctg ggcagtatca tgatcgtcac
     1141 attcaataag atgttccgcc attgccacct gcaacgctac ctgatgacgc gttttccggc
     1201 tttggttttt ccaccagtta cgcaaacctt cactaccgtt atcggcacgc gcctgatcca
     1261 tcaggccaat ccatgcctgt tgttccagca ttgcacgatg ttcttcatca ccaacatggg
     1321 ctttcgccat tgatgggatg atatccagca gagaactcca tgcccctgtg cggatatacg
     1381 cctgttccgc cagacgtaat acttcaggat ggcgtggcgt aacttccagc agcttatcca
     1441 cgccgtggcg tgcagcatgg ttttcattac gggccagttg cagacgtaca cgggtgattt
     1501 ctaccggaat ggtgtcattg ccggccagct ccgctgcgcg ttccagatgt tggttggcgc
     1561 gtgcttcatc accacgttgt tgcgcggctt cggcagccag tagatagttc accaccggtt
     1621 gttcagcgtg atcggcattt ttcgccatca gcttttcaac ttgctgataa tcaccttccg
     1681 ccagtttcag cagcgcctgt tcggtctgct tacgcgcacg gcgacgctta cgtccgacaa
     1741 accatccacg ggtgtgcgcg ccagtgcgga agatccgccg tagtagccac tcaatggcaa
     1801 acagcactac catcgccaga atcaatatga tcgccaggcc cgtgacgctg gtttcgatat
     1861 tgtagttgtc ggtctggatc agcacataac cctgatgacc ggcaatcatc gggccaacca
     1921 ctatccccgc aatcagcaaa acaaagagta ataacacttt tagcatggtt attctccttg
     1981 cggcgcggct gccggagtat caccttgcgg tgcaggtgca ggtgcaggtg caggtgcagg
     2041 tgcaggttta gcttccgttg tccccgctgc cggttgtgcc agcaggttac gcacgcgagt
     2101 ctgcatcagt ttttccagca tcgcctggct ttgcagggtt tccggaagat ccatcgagat
     2161 attttgctgg cttaactggt ccacctcgtc gaggaacgct ttggtggtgg catcatcagt
     2221 atcgtagtaa gcacgtaccc aggtggagac gttctccagc gcctggcgat aagtctcttc
     2281 ctgatgacgc ggtacagctt gtgctgcgac cagcaggcga gagcgaatat tttcgcgcag
     2341 atagatatcc tgatttggcg ctaacagcgg tacggcggtg tcatcacgac ggcgaatcgt
     2401 aatgaagttg tccataaagt tctgccagct tttttgcaga ttgatacgcc attcgctgat
     2461 ggaactggaa agctcttcac cgtctgaatc catcggcgaa ccatcgctgt cattatcggc
     2521 cagacgcagg ttatctacct gatttgaaag ctgattaagc ttaaggatga tgccgtcata
     2581 atccacctgc gatactgcag aaaggctggc gatatcatcg gtaattgccc gacgaacggt
     2641 aatcagactc gggtcattca tatccgccag gctggcgtct gcacttttca gcaacgctgc
     2701 agaggtcgtg acgtcctgat cgctccacag cttccgtccg gcgagtttca ccagaaaatc
     2761 ggcctgagcc agcagccagg ttttagcatc gctgccggaa atggtggcga tcttttgttg
     2821 gacttcatcc aactgttttg ccagcgtttc ttgctgacga ttcgcctgct taagctgcgc
     2881 agcttgttgc ttaataatgc cttccagctc ggctttttgg ctctcctggg ctttttgcaa
     2941 tgccgtcagt tggttagcca gggcatcgct ggtggcggtc tgattgacgg cctgttgttt
     3001 accccagcca tacaaaccga cacccgccgc cagagcaata gcgatagcca ccgcgctgag
     3061 aatcaatgca gtattgttct tactcttttt ttctgttgcg acaggttgtg acgtggtgtc
     3121 cacggcctcc ctggtctctt caaccacggc ggaggttttt tcttgttccg tcattatggc
     3181 ttcctgttat gagagttatt gtaatgcccg taaaagcgca tcgttgtcag cgttatcggc
     3241 gaccttaatg tcttgccagc ccagttcccg ggcgagtttc gccaaacgct cactgacgac
     3301 caatagtcga cagtgtagta accagtgctc acgataccat tgtgggatca gcgaccagag
     3361 ttgctgcaac atttcaccgc tggtaacaac gaccatcgtc acctcgcggg attgccagcg
     3421 catcgcttct tctgcaccat cgtaatggat tgcgcatcgt tgataacatt cacaaaaagt
     3481 gacctcagca ccgcgcaccg tcagggtatc cccaattagc tcacgaccgc cattgccacg
     3541 taatatcagc gcacgtttgc ccgcaatatt ttgtaattca ggtaattgta gcaagacttc
     3601 gctgatttcc cgatcctgtg ggtagagaat cttctgtccg cttacggtat gtagcgccag
     3661 tgcggtggtg cgtccaatgg cgaaataagt aggtagttgg ggccatttac gatcttgctg
     3721 atgcagctgt gattgggcaa aagcaaccgc gtgttgcgag agggcaaaca acagatcgct
     3781 ctcccccagc gctgccagtt gatcagcaag ttgcggtaat tgtcgacccg gagaaaactc
     3841 aatcagcgga aaatgccagg ccacctgccc cagtgtgcgc agacggctca ctaactcttc
     3901 tccagcggga gacgggcggg tgaccaggat actcatgccg gggcctctcc gttatagact
     3961 tcagcgagga tctcgcgcgc gccgttattc agtagctctt ctgccagcga aatccccatt
     4021 tgttcggcat cttgcggcgc accgcggcgt tcaccgcgaa taatctgcga accgtccggc
     4081 gcgccgacca gcgcacatag ccagatttcg ccatcaataa gctcggcgta gctaccaatt
     4141 ggcacctgac atccgccttc gagacgggta ttcatggcgc gttctgcggt aacgcgcagt
     4201 gcagtttcgt ggtgattcag cgcggcaagc agctcgcgag tgcgtgtatc atcaaggcgg
     4261 cattcaatac cgactgcacc ttgtcctacc gccggaagag aaatctcggg tggtaacgcg
     4321 gcgcgaatgc gggactccag acctaaacgt tttagtccgg ctacggcaag aatgatggca
     4381 tcgtattcgc cgttatctag tttgctcagg cgagtgccga cgttgccgcg cagggagcgg
     4441 ataatcagat ccgggcggcg ttcagccagt tggcactggc gacgtaaact ggacgtcccg
     4501 acgatactgc ctgctggtaa cgcatccaga ctgtcatagt tattggacac aaaggcatcg
     4561 cgaggatctt cacgctcgca aatagtgacc agccccagac cttgcgggaa ttcaaccggc
     4621 acatctttca ttgaatgtac ggcgatatca gcgcgattct ccaggagcgc gacttccagc
     4681 tcttttacaa ataagccttt tccgcctact ttcgccagcg gcgtatcaag aatcacatcg
     4741 ccgcgcgtca ccatcggtac cagttcaacg accaggcccg gatggctcgc catcaacttg
     4801 tctttgacat agtgtgcctg ccagagtgca agtgggcttt ggcgtgtggc aattcttaaa
     4861 acattgtcta acatgcttgt taccgtcatt atcatccgtg gtccatccta acatccttgc
     4921 cagagtgatg tcagtgttgt ggtgaaacgt agacgcctgc gcaaaccgta aaatgaggtc
     4981 tggcagtgga tcctgacagg cgtttcacgc cgtcgtaata aggaatttac agagaataaa
     5041 cggtgctaca cttgtatgta gcgcatcttt ctttacggtc aatcagcaag gtgttaaatt
     5101 gatcacgttt tagaccattt tttcgtcgtg aaactaaaaa aaccaggcgc gaaaagtggt
     5161 aacggttacc tttgacatac gaaatatccc gaatgccgcg tgttaccgtt gatgttggcg
     5221 gaatcacagt catgacgggt agcaaatcag gcgatacgtc ttgtacctct atattgagac
     5281 tctgaaacag agactggatg ccataaatca attgcgtgtg gatcgcgcgc ttgctgctat
     5341 ggggcctgca ttccaacagg tctacagtct actgccgaca ttgttgcact atcaccatcc
     5401 gctaatgccg ggttaccttg atggtaacgt tcccaaaggc atttgccttt acacgcctga
     5461 tgaaactcaa cgccactacc tgaacgagct tgaactgtat cgtggaatgt cagtacagga
     5521 tccaccgaaa ggtgagcttc caattactgg tgtatacacc atgggcagca cctcgtccgt
     5581 agggcaaagt tgttcctctg acctggatat ctgggtctgt catcaatcct ggctcgatag
     5641 cgaagagcgc cagttactac aacgtaaatg cagcctgctg gaaagctggg ccgcctcgct
     5701 gggtgtggaa gtcagcttct tcctgattga tgaaaaccgc ttccgtcata atgaaagcgg
     5761 cagcctgggg ggcgaagatt gtggctccac ccagcatata ctgctgcttg acgaatttta
     5821 tcgtactgcc gtgcgtctcg ccggtaagcg tattctgtgg aatatggtgc cgtgcgacga
     5881 agaagagcat tacgacgact atgtgatgac gctttacgcg cagggcgtgc tgacgccaaa
     5941 tgaatggctg gatctcggtg gcttaagctc gctttctgct gaagagtact ttggtgccag
     6001 cctttggcag ctctacaaga gtatcgattc cccatacaaa gcggtgctga aaacactgct
     6061 gctggaagcc tattcctggg aatacccgaa cccacgtctg ctggcgaaag atatcaaaca
     6121 gcgtttgcac gacggcgaga ttgtatcgtt tggcctcgat ccatactgca tgatgctgga
     6181 gcgtgttact gaatacctga cggcgattga agatttcacc cgtctggatt tagtacgtcg
     6241 ctgcttctat ttaaaagtgt gcgaaaaact cagccgtgaa cgcgcctgtg taggctggcg
     6301 tcgcgcagtg ttgagccagt tagtgagcga gtggggttgg gacgaagctc gtctggcaat
     6361 gctcgataac cgtgctaact ggaagattga tcaggtgcgt gaggcgcaca acgagctgct
     6421 cgacgcgatg atgcagagct accgtaatct gatccgcttt gcgcgtcgca ataaccttag
     6481 cgtctccgcc agtccgcagg atatcggcgt actgacgcgt aaactgtatg ccgcgtttga
     6541 agcattgcca ggtaaagtga cgctggtaaa cccgcagatt tcacccgatc tctcggaacc
     6601 gaatctgacc tttatttatg tgccgccggg ccgggctaac cgttcaggtt ggtatctgta
     6661 taaccgcgcg ccaaatattg agtcgatcat cagccatcag ccgctggaat ataaccgtta
     6721 cctgaataaa ctggtggcgt gggcatggtt taacggcctg ctgacctcgc gcacccgttt
     6781 gtatattaaa ggtaacggca ttgtcgattt gcctaagttg caggagatgg tcgccgacgt
     6841 gtcgcaccat ttcccgctgc gcttacctgc accgacaccg aaggcgctct acagcccgtg
     6901 tgagattcgc catctggcga ttatcgttaa cctggaatat gacccgacag ctgcgttccg
     6961 caatcaggtg gtgcattttg atttccgtaa gctggatgtc ttcagctttg gcgagaatca
     7021 aaattgcctg gtaggtagcg ttgacctgct gtaccgcaac tcgtggaacg aagtgcgtac
     7081 gctgcacttc aacggcgagc aatcgatgat cgaagccctg aaaactattc tcggcaaaat
     7141 gcatcaggac gccgcaccgc cagatagcgt ggaagtcttc tgttatagcc agcatctgcg
     7201 cggcttaatc cgtactcgcg tgcagcaact ggtttctgag tgtattgaac tgcgcctttc
     7261 cagcacccgc caggaaaccg ggcgtttcaa ggcgctgcgc gtttctggtc aaacctgggg
     7321 cttgttcttc gaacgcctga atgtatcggt acagaagctg gagaacgcca tcgagtttta
     7381 tggcgctatt tcgcataaca aactgcacgg cttgtcagtg caggttgaaa ccaatcacgt
     7441 caaattaccg gcggtggtgg acggctttgc cagcgaaggg atcatccagt tctttttcga
     7501 agaaacgcaa gacgagaatg gctttaatat ctacattctc gacgaaagca accgggttga
     7561 ggtgtatcac cactgcgaag gcagcaaaga ggagctggta cgtgacgtca gtcgcttcta
     7621 ctcgtcatcg cacgaccgct tcacctacgg ctcaagcttc atcaacttca acctgccgca
     7681 gttctatcag attgtgaagg ttgatggtcg cgaacaggtg attccgttcc gaacaaaatc
     7741 gattggcaac ctgccgcccg ccaatcagga tcacgatacg ccgctattac agcagtattt
     7801 ttcgtgatga gcgtgccgga aagcgaggct tatccggcat gcaatcttag cggaaactga
     7861 ctgtttcacc cgcctgttgc gtcgccgcct gttccagcaa atcccagaag gtttcgccgc
     7921 tgcgatcgca aatccattca tcgcctttca ggtcaaaatg gtagccgccc tgtttggttg
     7981 ccagccacac ctggtgcagc ggctcctggc ggttaataat gattttgctg ccgttttcaa
     8041 aggtaatggt cagtacgccg ccgttgattt cgcagtcgat atcgctgtcg ccatccctgt
     8101 cgtccagacg ctcttcaatg gtcagccaca gttgatcagc caggcgatga aattcactgt
     8161 cgttcattgt tgtatcctgt ttttaagtga tggcggcagt atagcggcat ggggtcaggg
     8221 cttcaaagtt tgcacctctg ctgctgcgtt ccggcatgat tcatccgtca ccggaataat
     8281 gatgtctctg ttttgaactt cacgtacgtt ctcctctctg acacgagttt tcgccgggca
     8341 gggggagggc gtaatcctta cctctgtatc ggtccatgta taaacattgc gatcgtcata
     8401 agcgaagcga tcgtttttag ttagcgaacc gttaactgga ataatttgca agctctttga
     8461 ccgtaattgg cggataaagt aagcatgctg tttgccgcta ttaaatgagc gatcaatgac
     8521 caggtctggc gtatccagcg ggattatctc aagctggtct tcaccaaatg tcgataattt
     8581 tgttacatac agccgatctt tatattgcgc aatattgcca gtaatcagat ggaatt
//
LOCUS       DAOFMX010000139         8583 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.140, whole genome shotgun sequence.
ACCESSION   DAOFMX010000139 DAOFMX010000000
VERSION     DAOFMX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.140"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(541..888)
                     /locus_tag="QBA20_003895"
     CDS             complement(541..888)
                     /locus_tag="QBA20_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6294248.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAAKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTIV"
     gene            complement(988..1332)
                     /locus_tag="QBA20_003896"
     CDS             complement(988..1332)
                     /locus_tag="QBA20_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294249.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFGKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(1359..1526)
                     /locus_tag="QBA20_003897"
     CDS             complement(1359..1526)
                     /locus_tag="QBA20_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294250.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHAEVTNI
                     RIEELKEHQSA"
     gene            complement(1627..2160)
                     /locus_tag="QBA20_003898"
     CDS             complement(1627..2160)
                     /locus_tag="QBA20_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDQ6294251.1"
                     /translation="MSTITREWLQQAINDYESVRDELPFGLDDYQGNILAALRIALAS
                     LEAEPVAWKVTFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQQVP
                     VTPDGWISCSERMPEIRQTVIGWNGYAVRQCVYTRNEYAKTQKGREPRFETLTGIWHG
                     VTHWMPLPEPPQEVNRG"
     gene            complement(2157..2891)
                     /locus_tag="QBA20_003899"
     CDS             complement(2157..2891)
                     /locus_tag="QBA20_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDQ6294252.1"
                     /translation="MSQIDYQALREKAEKATCGAWSLEYGEGQFDGDDALIHREVAGY
                     IPICRIEGAHPESGFDEDFQMEQQANAEFIAAASPATVLALLDERERNQQYIKSRDQE
                     NEDIALTVGRLRVELEGKDSKIANLTAERDALREGEMGDARHSNTRAAADIYFQLVEE
                     CEIPAGGSLVEYVDDMREKLEAAEKRIAELELREVVLPQCYSMLHRVDFDKPYHTEMV
                     YRQHQVLEALHNAGINVTEAGKGEAS"
     gene            complement(2888..3133)
                     /locus_tag="QBA20_003900"
     CDS             complement(2888..3133)
                     /locus_tag="QBA20_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294253.1"
                     /translation="MSNSARLQLGFSPLSKTIMLAKMRDVEGGRMRVGNDPGRDVTNE
                     AAQLVWRLVMAEGGEIAWELDDGSRMVLKAEKQGGNQ"
     gene            complement(3130..3297)
                     /locus_tag="QBA20_003901"
     CDS             complement(3130..3297)
                     /locus_tag="QBA20_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HDQ6294254.1"
                     /translation="MRGLAYNPGILPAKMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMLRKGGNQ"
     gene            complement(3308..3601)
                     /locus_tag="QBA20_003902"
     CDS             complement(3308..3601)
                     /locus_tag="QBA20_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HDQ6294255.1"
                     /translation="MPAPLYGADDARRCSGNSVSEVLDKFRKNYDLIMSLPQETKEEK
                     GFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(3615..4121)
                     /gene="ssb"
                     /locus_tag="QBA20_003903"
     CDS             complement(3615..4121)
                     /gene="ssb"
                     /locus_tag="QBA20_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDQ6294256.1"
                     /translation="MASRGVNKVIILGRVGQDPEVRYSSSGTAFANLTIATSEQWRDK
                     NTGEQKELTEWHRVAVSGKLAEVVGQYVKKGDQIYFEGMLRTRKWKDQSGQDRYTTEV
                     HVGINGVMQMLGGIGDSKQQAASRQSQKPQQQSSPAQHNEPPMDFDDDIPFAPVTLPF
                     PRHAIHAI"
     gene            complement(4122..4829)
                     /locus_tag="QBA20_003904"
     CDS             complement(4122..4829)
                     /locus_tag="QBA20_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDQ6294257.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIKIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKQGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQVA"
     gene            complement(5084..5236)
                     /gene="kil"
                     /locus_tag="QBA20_003905"
     CDS             complement(5084..5236)
                     /gene="kil"
                     /locus_tag="QBA20_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HDQ6294258.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(5221..5352)
                     /locus_tag="QBA20_003906"
     CDS             complement(5221..5352)
                     /locus_tag="QBA20_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HDQ6294259.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            complement(5377..6345)
                     /locus_tag="QBA20_003907"
     CDS             complement(5377..6345)
                     /locus_tag="QBA20_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HDQ6294260.1"
                     /translation="MSEVTDLVVIEKANAMTVFKSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVRDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKVRAEAEAKAKAEIEAAARREAEAKAAAERAERERIEAEQRAQ
                     REAKEAAERAEREKHAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILVDLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            complement(6480..6680)
                     /locus_tag="QBA20_003908"
     CDS             complement(6480..6680)
                     /locus_tag="QBA20_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294261.1"
                     /translation="MCEFYEADIKRPEMASDATLRDYFAAKAMQAMISNPSIIDNDSD
                     GAVNYAASAAYKFADAMLKARE"
     gene            complement(6680..6976)
                     /locus_tag="QBA20_003909"
     CDS             complement(6680..6976)
                     /locus_tag="QBA20_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294262.1"
                     /translation="MEFKGTEGKWEIMMDGDEIKIIQADSLENGAGWRSYIAICEEVQ
                     CIEDANLIAAAPDLLEALQLLLKQSKNRTTTTYPEWYGAVNKGLAAISKALGDE"
     gene            complement(7014..7637)
                     /locus_tag="QBA20_003910"
     CDS             complement(7014..7637)
                     /locus_tag="QBA20_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294263.1"
                     /translation="MSSIRLTTRMKEEIARNALIKSGVFTELEEVTKLKNQLALDARV
                     IAFGGKKKTEEVDQLSSKLVAISEELEKMGCSFYSYEFRSTSIYLTVSGRRVGWHSYG
                     KDGNGEDIFLPTPTKDKCMFDAEHKITKRFDEICALQQKLEAKKKDIESNVWAALNSV
                     TTVKRLIEVWPESKELLPKEADKASTALPALRVEDLNKMIGLPSEVA"
     gene            complement(7674..7874)
                     /locus_tag="QBA20_003911"
     CDS             complement(7674..7874)
                     /locus_tag="QBA20_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HDQ6294264.1"
                     /translation="MRMTIDTNQWCGQFKRCNGCKLQSECMVKPEEMFPVMEDGKYVD
                     KWAIQTTAMIARELGKQNNKAA"
     gene            complement(8229..8501)
                     /locus_tag="QBA20_003912"
     CDS             complement(8229..8501)
                     /locus_tag="QBA20_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294265.1"
                     /translation="MAVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIALKLSG
                     CVRADKAASLGSLRCKKADECSGSICLPNVAIYAAGYRKSKQLTAR"
BASE COUNT         1863 a         2215 c         1767 g         2738 t
ORIGIN      
        1 caaggtctgt aacgttggaa cggcgaaggc ctgtcgtgat tgcaaacatg accacaggga
       61 agaaatgagg agcaatttct gcaaacaggc gcttcgattc ctcctctgta agccatctga
      121 ttcgtccatt cttaacgcgt ggtgttgata ttttgggcgc cctgtcaagc catccccatt
      181 caacagccat attgagaata gcgcgaagta ttgccagatg ccgcgtcttc gttcctttgc
      241 ttgccagctt tggtttatac tccggcactg gcttgccaag ccgcaaacac ctgtcccggc
      301 tcatttccca gttcaggcga tggcggcggt tttccatccc gtctaccgcc tccattattt
      361 tttctgttgt tatgtcagag agaatggttt ctctgaagtg caacatccag aacgatataa
      421 tgctcttgtc atcatcaatg gacttcttat ccgatttctc acgcagccac cgtatgcagg
      481 cttccttgaa tagctttttc ggcgattccc cgagattttt tactctccac gcttctgctt
      541 tcagacgatc gtgaagttct tgcgcttgcc ttttgtccga tgtttcaaga gagcgtctaa
      601 ctcttgatcc atctggcgcg acgaaatcgc agtgccacgt gccaccgcgt agtttgattg
      661 acatgcttta acctcctgca catcaaccgc attcaccgcg ctattgtgtc tcacagactt
      721 aagcgccgca atgcagtctg acttgcaaat gcgatatgga cttttaggtt tatctggatt
      781 tatctttgcg gcctgaagtc gtccacttcg tatccactgt gtgatagtgc ctttgtctac
      841 cttcagatac gacgcagctt cttcacgagt gaagatttct tcttccacct ggaatctcca
      901 tttgttggat tgacatgatt gcggtaggtc tggatatctt gagaaatgaa ctggcctcat
      961 cgagtgtgag gctgtgtgat tccatagtta gtccttgcgt agctcgctga ttcttctgta
     1021 agtctctggt gctttgtttc cgtgtatctt catttcagac ttcaacagag caacgaggga
     1081 atcccattcg ttgaggatgc ctttgaatgc cggaacgcgc tttgcaacct tgccgaatga
     1141 atctctgatt tctggaatct gctcaacaag tgcaacgcat cgtctgaaat cggctgcgtc
     1201 atggggagcg ccgaagtgat gaccatagat attctttttc aggccacatg cgattgaggc
     1261 aagagttgcg ctactgatgc caacatcgcc agtcgattgc catttcaaaa ccttcatagc
     1321 caaatctgac atttcttgtc tccaataaaa aaccgccatc aggcggattg gtgttctttc
     1381 agttcttcaa ttcgaatatt ggttacttct gcatgtgcta tctgcgccca tatcatccag
     1441 tggtcatagc agtcgttgat gttctctgct tcgataactc tgttgaatgg ctctccattc
     1501 cattcacctg tgactcggaa gtgcatttat catctccata aaacaaaact cgccgtagcg
     1561 agttcagata aaagaaatcc ccgcgagtgc gaggattgtt attcaccttt gacggcaagt
     1621 tgtaggttag ccacggttaa cctcctgagg cggttctggt agaggcatcc agtgggttac
     1681 tccatgccat ataccagtta aggtttcaaa ccttggctct ctgcctttct gtgtcttggc
     1741 atattcattc cttgtatata cgcattgtct aactgcatat ccattccatc caataactgt
     1801 ttgtctaatt tccggcatcc gctcactaca gcttatccaa ccatccggag ttaccggcac
     1861 ttgctgggcg gtataaagcg gtgttatatc tgcccgaaaa ttacatgctt tatgcagccg
     1921 cacccaccgt tcgacttctg ctttgtcaga atacatacca gtgaacgtgt tatattcacg
     1981 gtcaatttgc gtgaatgtta ccttccacgc caccggctct gcttccagcg atgccagtgc
     2041 gatacgcagg gcagccagga tattcccctg gtaatcatca agcccgaaag gaagctcatc
     2101 acgaacgctt tcataatcgt tgatagcctg ctgcagccat tctcttgtga tagtgctcat
     2161 gatgcctctc ctttacctgc ttcggtgacg tttattccag cgttgtgcag tgcctcaaga
     2221 acctgatgct gcctgtaaac catttcagtg tggtaaggct tgtcaaaatc gacgcgatgc
     2281 aacatgctat agcattgcgg aagcacaacc tcccgcaact ccagctcagc aatgcgcttc
     2341 tctgcggctt ccagcttctc gcgcatatcg tcaacgtact cgaccagaga tccgccagca
     2401 ggaatttcgc attcctcgac cagttggaag tagatatcag ctgcggcccg tgtgttgcta
     2461 tgcctagcgt cgcccatctc accttcacga agagcatcgc gttcggcggt aagattggct
     2521 attttgctgt ctttgccttc cagctcaacg cgcagcctcc caaccgtaag cgcaatatcc
     2581 tcgttctcct ggtcgcgtga tttgatgtat tgctggtttc tttcccgttc atccagtaat
     2641 gccagcacgg tagctggact ggctgctgcg atgaattcag cattggcctg ctgttccatt
     2701 tggaaatctt catcgaaacc gctttcagga tgcgctcctt caattctgca aatgggaata
     2761 tatccagcaa cttctcgatg aattagcgca tcatcaccat caaattggcc ctctccatat
     2821 tcgagcgacc acgccccaca cgttgctttc tctgcctttt cacgcagtgc ctgataatca
     2881 atctggctca ctggttgccc ccctgctttt ctgccttcaa caccatgcga gaaccatcat
     2941 ccaactccca agcgatctca ccaccttcag ccatgaccag tcgccacacc aattgagcag
     3001 cctcattggt aacatcacga cctggatcat tgccaacgcg catacgtcca ccttcaacat
     3061 cgcgcatttt tgccagcatg atagtttttg atagcggtga aaaaccaagc tgtagtcgtg
     3121 ctgaattact cactggttgc ctcctttgcg caacatcgca ttcagatatt tgttttgatt
     3181 tactgatgga aaagaatttc tcttaagcaa ttcctctctc gatggcattg gctttacgcg
     3241 ttggcgaata atcatttttg ccggaagaat gccgggattg tatgcaagtc ctctcatggt
     3301 aaattcctca gtcattactg atagcgccat agcgtgagcg gtaattacgc aggcgcgggt
     3361 caatttcagg gaagtgggtg tatgtggctt tgcggaatgg tcggattgat gtctggtaaa
     3421 ttcgctcgcg ttcttctttc tctgcaagcc atatacagtg gcgaaatccc ttttcctctt
     3481 tcgtttcctg cggtagcgac attatcaggt cgtagttttt cctgaattta tccagcacct
     3541 ccgatacgga attgccggaa cagcggcgcg cgtcgtccgc accatataga ggcgctggca
     3601 tgattttctc ctgattaaat tgcgtgaata gcgtgacgag ggaaggggag agttactggt
     3661 gcaaagggta tatcgtcgtc aaaatccata ggaggttcgt tgtgttgtgc tggtgatgat
     3721 tgctgctgtg gcttctgtga ttgcctgctg gctgcttgtt gtttgctgtc gccaatgccg
     3781 ccaagcattt gcatcacgcc attaattccg acatgaacct cggttgtgta acggtcttgc
     3841 cctgactggt ctttccactt tctggttctc agcattccct cgaaataaat ctgatcacct
     3901 tttttcacat actgccccac gacctcagcc agtttcccgg atacagcaac acgatgccat
     3961 tcagtcaatt ccttttgctc gccagtattt ttatctcgcc attgttctga tgtggctatt
     4021 gtcaggttag cgaacgctgt tcctgatgat gagtatcgga cttccgggtc ttgtcctacc
     4081 cgaccaagga taatcacctt atttacgcct ctgcttgcca tttatgccac ctgttttagt
     4141 tcgttaactc tgatgttcat tacctgaacg catttagcct gcgcctcctc gttgccagcc
     4201 attaattgcc agtcacgctg ataacgctcg atgagttttt tcttgtcagt ttctgttgac
     4261 gcataatcgc tgaagtcttt caggatttgc tcgcagtcaa ccgatggaga tttctggttg
     4321 gtattttctg gtgatggttt gttatctgat gctgggattg cccatcccgg cagcgatgga
     4381 gggagccagt aaaatcctgt tccatccttg agttttgccc tgtgccatcc ctgcttttta
     4441 tcgagagatg tttgtgcgaa accttcctca aggttataca gataccgacc gattccccac
     4501 tgaacggcag cgcgcttcat tgcaccggaa cgaccacctt tgacggcttc tacctgcgtg
     4561 ttttcagcag catcccattt ggttacccat tcggaatcaa tcttgattga tatgccgcat
     4621 tcaacgccgc cgttgttggg aatatcgcgg tattcattgc gccatcctgc tttgccgcaa
     4681 acatcgtcca ggcgtttcat gattgccctg ttcgtgacat aagccagcac catagcccac
     4741 acttttccat cgcgtgtttt accgctttgc tgtattcgcc attcgatatc ttcagggctg
     4801 aatggctcat cgaatttatt caaatccata attcacctca gaatggacat ggcccaagga
     4861 aataacgctg gtttaatact tcgactctgg acaaattaag gcatacccgc attccttcgc
     4921 ggtcgccatt atggcgatac cagagagctt tctgcgtgta catgcgtctc tgtaacttgc
     4981 tctccttcac tgtggttgca agtgacatgc atatctcctt cgttaccgat taattctttc
     5041 atctgacgaa tgaattcttc gtctgaccag ttatctgtaa aactcatttc ctgcgatacc
     5101 acggaaggtt gatagctgat ttcatcgctt tatttgcttc aagccacatt tttgaatcac
     5161 caataaatct ggctattact gctttgttct gtgcagcacg aagcatctgg tgattgatgg
     5221 ctatttcatt gcgcataata agacctcaac tcttttccat ccgtcacgta atttacgggt
     5281 gattcgctca agtaaagatt catttagttg gaaggcaccc atgcgagcgc ctcccgcgat
     5341 tgcgtaaatc atgggtggtt ccttatattg gttttattag taggttattt ttgttgcgaa
     5401 tacttcgcct tttacgatgg ctgttatgat atttttagca acatcttctg atgcaccaac
     5461 cttgataagg tcaacaagta ttttgttatt tacttctttc cggtgagctt tatcttttgc
     5521 tctacgctct tcttcgtcct tgattctttt ttcttctgct attctggctt gctcttttgc
     5581 ttcagcctcg cgacggattc gttcagcctc ctcctgtgct tttctgcgtt ctgcttcaat
     5641 tgccgcatgc ttttctcttt cagctcgttc cgccgcctct tttgcttcgc gctgtgctcg
     5701 ttgctcggct tcaatgcgtt cacgctctgc tcgttccgca gcggccttag cttctgcttc
     5761 tcgccttgct gctgcttcaa tttcggcttt tgcctttgct tcggcttctg ctctgacttt
     5821 ctcttcagct tctcttttta agcgttcttc atgctctcgc ttttcctgct ccgctttgag
     5881 tcttgcctct tctctttggc ggtcaaattc gcgatccatc aaaatcgcta tttcatggtc
     5941 agactcaatt tgctttgcga gagcttcagc tgctgcctta gcttcttctt cggctttaat
     6001 ccgcgcctgc tcctcctcat aatcagtaag aggctggcgc gccttggctt tcagttcatc
     6061 aaggcgatca cgcactgtct tgcggttggc atcaattagc tttggaattt ccttcagttc
     6121 agcaacaagg tctttgccaa gaccatcgag atatgttttc gtctgcgcaa ctttatacgc
     6181 cagagaagcg atctcctttc tgccctttgc cgttgtgata tcaggcacaa aggacataac
     6241 ttcacgttca accttttgga gaatttcttc aatctggtcg gcagacttaa atacagtcat
     6301 tgcatttgct ttttcaataa caactaaatc tgttacttca ctcatatatc ctccatcaaa
     6361 aaaattgccc tcacactgga gggcaaagaa gatttccaat aatcagaaca agtcggctcc
     6421 tgtttagtta cgagcgacat tgctccgtgt attcactcgt tggaatgaat acacagtgct
     6481 tattcgcgag ctttgagcat tgcgtctgca aacttatatg cagcgcttgc tgcataatta
     6541 acagctccat cagaatcatt atcgataatc gatggattgc taatcattgc ttgcatagcc
     6601 tttgccgcga agtaatcacg taatgtcgca tcacttgcca tttctgggcg tttgatatct
     6661 gcttcataaa actcgcacat cattcatctc ccagagcctt gctgattgct gcaagacctt
     6721 tattaacagc tccataccat tctggatatg ttgtcgttgt tctatttttg gattgcttaa
     6781 gtaataactg aagtgcttcg agaaggtcag gtgctgccgc tattagattg gcatcttcaa
     6841 tacattgaac ttcctcacag attgcaatat acgaacgcca gcctgcgcca ttttcaagtg
     6901 agtctgcctg gatgatttta atctcatcgc catccatcat tatttcccac ttaccttcag
     6961 tacctttaaa ttccatgtta gcctctgttg tttatgccaa aaataaaggc cgactatgcg
     7021 acctcggaag gaagtccaat catcttattc aaatcttcta cccgtaaagc aggaagtgct
     7081 gtacttgctt tatctgcttc ttttggtagc aattctttgc tttcaggcca aacttcaata
     7141 agtcgcttaa ctgttgtgac tgagttcaaa gcagcccata catttgattc gatatccttt
     7201 ttcttggctt caagtttttg ttgcaatgcg cagatttcat caaacctttt tgttattttg
     7261 tgttctgcat caaacatgca tttatctttg gttggagtag ggagaaatat atcttcgcca
     7321 ttgccgtctt tcccatatga atgccatcca acccttctgc cagatacagt cagataaatt
     7381 gaagtagaac gaaattcgta tgagtaaaat gaacatccca tcttttcaag ttcttcactt
     7441 atagctacca acttggatga taactgatcc acttcctcag ttttcttttt accgccaaac
     7501 gcaataactc tggcgtcaag tgcaagctgg ttctttaact ttgttacttc ttcaagttca
     7561 gtgaaaaccc cagacttaat taaagcgtta cgagcgattt cctctttcat tctcgtagtt
     7621 aagcggattg atgacatatt aattcctctc aaataagaaa ataaaggcca ccatcaggca
     7681 gccttgttgt tctgtttacc aagttctctg gcaatcattg ccgtagtttg tattgcccat
     7741 ttatcgacat atttcccatc ttccattaca ggaaacattt cttcaggctt aaccatgcat
     7801 tccgattgca gtttgcatcc attgcatcgc ttgaattgtc cacaccattg atttgtatca
     7861 atagtcatac ggatagtcct ggtattgttc catcacatcc tgaggatgct cttcgaactc
     7921 ttcaaattct tcttccatat ctcacctcaa ataagtggtt tgctgccaaa acaatgaacc
     7981 atccggaaat tccagatagt tcataattca ctcttcaata cttccaactt actaatcgcc
     8041 gatagatatc cgcgctgata gggcatcatc attccttcga gcttgccact tcttaactcc
     8101 tccctgagca attgtattgc ttgatcaata acctctgcct tagcgtcctt tatggcttgc
     8161 ttgcggggct ttgctttctg ctttggcaga tttctcaagc atgatggaat gtatgtctga
     8221 ttcaccactt acctcgctgt cagttgtttt gatttccggt agcctgccgc gtaaatggct
     8281 acgtttggca ggcaaatact tccactgcat tcatctgcct tcttgcagcg aaggcttccg
     8341 agtgatgctg ctttatctgc tctgacgcaa ccagagagct ttagcgcaat ttttcgtgcc
     8401 agtcgctgct cttgcattgc ctgttcacgt tgagcctgtc tgcgtgctct gcggcgattt
     8461 ctggcgttat cgtcagccag atatgtaatg actactgcca tgttgacctc cgatgattga
     8521 ctttggcggt gacgcgacgg gtgcttatct tccggttgct gtcgtgcagc tgcacttcac
     8581 gtc
//
LOCUS       DAOFMX010000140         8314 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.141, whole genome shotgun sequence.
ACCESSION   DAOFMX010000140 DAOFMX010000000
VERSION     DAOFMX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.141"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..159
                     /locus_tag="QBA20_003913"
     CDS             <1..159
                     /locus_tag="QBA20_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294266.1"
                     /translation="ISESGEVVDMAAYEAWQQENHPDTWLQMQRREEVNINVHRERGE
                     DVEPGDDF"
     gene            complement(168..566)
                     /gene="vapC"
                     /locus_tag="QBA20_003914"
     CDS             complement(168..566)
                     /gene="vapC"
                     /locus_tag="QBA20_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HDQ6294267.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="QBA20_003915"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="QBA20_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HDQ6294268.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..1246
                     /locus_tag="QBA20_003916"
     CDS             875..1246
                     /locus_tag="QBA20_003916"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HDQ6294269.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALAST"
     gene            1267..6112
                     /gene="traI"
                     /locus_tag="QBA20_003917"
                     /pseudo
     CDS             1267..6112
                     /gene="traI"
                     /locus_tag="QBA20_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6132..6878
                     /gene="traX"
                     /locus_tag="QBA20_003918"
     CDS             6132..6878
                     /gene="traX"
                     /locus_tag="QBA20_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HDQ6294270.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6933..7493
                     /locus_tag="QBA20_003919"
     CDS             6933..7493
                     /locus_tag="QBA20_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HDQ6294271.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7624..7836
                     /locus_tag="QBA20_003920"
     CDS             7624..7836
                     /locus_tag="QBA20_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDQ6294272.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8080..>8314
                     /locus_tag="QBA20_003921"
     CDS             8080..>8314
                     /locus_tag="QBA20_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDQ6294273.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTV"
BASE COUNT         1909 a         2080 c         2667 g         1658 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggctgca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcctgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc actggcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcacggcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgctggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggggc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aacgccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagtgtcaca ctttctgacg gtcagcagac
     4981 ccgtgtgatt cgccccggtc aggagcgggc agagcaacat attgacctgg cctatgccat
     5041 caccgcccac ggtgcacagg gggcgagtga aacctttgcc atcgcgcttg aaggcacgga
     5101 aggtaaccgg aaactgatgg ccggctttga gtcagcctac gtggccctgt cgcgtatgaa
     5161 gcagcatgtg caggtgtaca ccgataaccg tcagggctgg acggatgcca tcaacaatgc
     5221 cgtacagaaa ggaactgccc acgatgtgtt tgagccgaaa ccggaccggg aggtcatgaa
     5281 tgcagagcgg ctgttcagta cggcgcggga gctgcgggac gtggcggcag gccgtgccgt
     5341 tctccgtcag gcggggctgg ccgggggaga cagtcctgca cggtttattg ctccgggacg
     5401 taaatatccg caaccgtatg tggcactgcc ggcgtttgac cgtaacggca agtccgccgg
     5461 tatctggctg aacccgctga ccacggatga cggaaacggg ctgcggggat tcagcggtga
     5521 aggtcgggta aaaggcagcg gggatgcgca gttcgtggcc ctgcagggca gccgtaacgg
     5581 agaaagcctg ctggctgata atatgcagga tggcgtccgg attgcccgtg ataatcctga
     5641 cagtggtgtg gtggtaagaa tcgccggtga aggccgtccg tggaatcccg gtgccatcac
     5701 cggtggccgc gtgtgggggg atatcccgga caacagcgtc cagccgggag ccggaaatgg
     5761 tgaaccggtc acggcagagg tactggcaca gcggcaggct gaagaggcca tccgccgtga
     5821 aacggaacgt cgcgcagatg aaattgtccg taaaatggca gagaacaaac ctgacctgcc
     5881 ggacggcaaa acagagcagg ctgtcaggga aattgccggg caggagcgtg accgggctgc
     5941 cataactgaa cgggaagccg cgctgccgga gagtgtactg cgtgaaccac aacgggtgcg
     6001 ggaagcggtc cgggaagttg cccgggaaaa tttgctgcag gagcgtctgc agcagatgga
     6061 gcgggatatg gttcgtgacc tgcagaaaga gaaaaccccg ggcggagact gatacaggaa
     6121 gacaaacact gatgacaacg gataacacga acacgacacg taacgattca ctggctgcac
     6181 ggaccgatac ctggttgcag tcatttctgg tctggtcacc cggacagcgg gatatcatca
     6241 aaacggtggc actggtgctg atggtactgg accacatcaa tctgatattc cagctgaagc
     6301 aggaatggat gtttctggcc gggcgaggag cctttccgct ttttgccctg gtgtgggggc
     6361 tgaatctgtc ccgtcatgcg catatccggc aaccggccat taaccggctg tggggatggg
     6421 gaattattgc ccagttcgca tattatctgg caggctttcc ttggtatgag gggaatatcc
     6481 tgtttgcctt tgcagtggcg gcacaggtgc tgacgtggtg tgaaacgcgc agtgggtggc
     6541 gtacagcagc ggccatactc ctgatggccc tgtgggggcc tttgtccggc accagttacg
     6601 gtattgccgg gttgctgatg ctggcagtca gcaaccggct gtaccgggcg gaagacagag
     6661 cggaacgtct ggcgctggtc gcctgcctgc tggctgttat tccggcgctt aaccttgcca
     6721 ccagtgatgc agcggcggtg gccggtcttg tgatgacggt gctgaccgtt ggtctggtgt
     6781 tgtgtgccgg aaaatcatta ccccgtttct ggcccggaga ctttttcccg acgttctatg
     6841 cctgtcatct ggctgtgctg ggcgttctgg cgctgtgacg ggtgtggtat ctttggccgc
     6901 aagaggatga ttcgtcagag gcagaacaca gcatgacaga gcagaagcga ccggtactga
     6961 cactgaagcg gaagacagag ggaacagcgc ctgtccgcag ccggaaaacc atcatcaatg
     7021 tcaccacgcc accaaagtgg aaggtgaaaa agcagaagct ggccgagaaa gcggcccgtg
     7081 aagcagagct ggcggcaaaa aaagcgcagg ccagacaggc gctgtccatt tatctgaacc
     7141 tgcccacgct ggatgaggct gtgaataccc tgaagccctg gtggccggga ttatttgacg
     7201 gtgacacgcc ccggcttctg gcctgcggta tccgggacgt gttactggaa gacgtggcgc
     7261 agcggaatat cccgctctcg cataaaaaac tgcgcagggc gctgaaggcc atcacccgtt
     7321 cagaaagcta tctgtgtgcc atgaaagccg gtgcctgccg gtatgacacg gaagggtatg
     7381 tgacggagca tatttctcag gaggaggaag cgtatgcggg agcgcgtctg gcgaaaattc
     7441 gtcgccagaa ccggataaag gcagaacttc aggccgtgct tgatgagaaa taaaaaaagc
     7501 ctcccccaga gagaggaggc agggaaataa ggcttaaaag gaaataactt ccgcttccga
     7561 aaggaagcca gtccactgaa ttatttatca caataatact gacaactaaa gggggacaag
     7621 aaaatgaatg gattcagaaa cagttcacgg aacggccagg tctggcgtta ccagcgtgcc
     7681 ggcgggcggg ccgttattct ggaagtcagt ggacgctgga tggaagcggc agaagcatgg
     7741 cgacgggctg cctgtgttgc gcctaggaca gactggcagc agtttgcccg gaaaagggct
     7801 gaacactgcc accggcgctg ccggggaagg gtgtaacgaa aaaagcagcc ttcattactc
     7861 taggagtcag aaggctgaaa ggagtttatg agatttgctt cttaatatcc ggtcgggcag
     7921 tatgaccgga cagttctgag tagcacagag tcttttccgc attaagtcga ttttttatat
     7981 tttgtgatta gtttcacaaa ataaggtgtt attcaatgtg tgctgcacta ttcagggggg
     8041 ctgaaataca ggtggcattt atctgatatg gagaataaca tgaggaaata tattccactg
     8101 gtattattta tcttttcatg gccggtattg tctgcagata ttcatggacg ggttgttcgt
     8161 gtactggacg gtgataccat cgaggtcatg gactcactga aggcggtgcg gatcaggctg
     8221 gtaaatattg atgcaccgga aaagaaacag gattatggcc ggtggtcaac agacatgatg
     8281 aaatcactgg tcgcaggaaa aacggttacc gtca
//
LOCUS       DAOFMX010000141         8225 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.142, whole genome shotgun sequence.
ACCESSION   DAOFMX010000141 DAOFMX010000000
VERSION     DAOFMX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.142"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..305
                     /locus_tag="QBA20_003922"
     CDS             <1..305
                     /locus_tag="QBA20_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDQ6294274.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            277..2205
                     /locus_tag="QBA20_003923"
     CDS             277..2205
                     /locus_tag="QBA20_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDQ6294275.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2189..2392
                     /locus_tag="QBA20_003924"
     CDS             2189..2392
                     /locus_tag="QBA20_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6294276.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2389..3982
                     /locus_tag="QBA20_003925"
                     /pseudo
     CDS             2389..3982
                     /locus_tag="QBA20_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3972..5477
                     /locus_tag="QBA20_003926"
     CDS             3972..5477
                     /locus_tag="QBA20_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDQ6294277.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPDGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASDRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5514..5861
                     /locus_tag="QBA20_003927"
     CDS             5514..5861
                     /locus_tag="QBA20_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDQ6294278.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5919..6947
                     /locus_tag="QBA20_003928"
     CDS             5919..6947
                     /locus_tag="QBA20_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDQ6294279.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6999..7385
                     /locus_tag="QBA20_003929"
     CDS             6999..7385
                     /locus_tag="QBA20_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDQ6294280.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7397..7774
                     /locus_tag="QBA20_003930"
     CDS             7397..7774
                     /locus_tag="QBA20_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6294281.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7761..>8225
                     /locus_tag="QBA20_003931"
     CDS             7761..>8225
                     /locus_tag="QBA20_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6294282.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1905 a         1934 c         2542 g         1844 t
ORIGIN      
        1 tactcattgc cagatgggaa ctgaccgcag aacaggctgt cgcacagcag ttaaaaaatc
       61 aggtgtcaaa aggaaatctt atcgataccg gattttgtat ttttgccctt agtaagctgg
      121 cgatggcttt atccagtacg cttgactcca ttcctttgtc catgcaacga cagttcccgg
      181 atttaacgcc gcgtcatatt gatcatctga aaatccttat tgctaaggga gcgaatcagt
      241 gcgcgcgtgc gggggataaa ttaccggatt tactcgatga atatatcaga acaacaactg
      301 agtaatatga tggtggcagt cactatcgca ctgcagccac tggtcagggt attgccggtg
      361 acggctgtag aatgggccga tcaaaattat tacctgccta aagaatcgtc atacggtgag
      421 ggggaatgga aaacgctgcc gtttcaggtc gccatcatga actgcatggg caatgacgag
      481 attcgtacag ttaacctgat taaatctgct cgtgttggtt atacaaaaat ggtgcttggt
      541 gtgatcgggt attttattga gcacaaatcc cgcaacactc ttctttttca gccgacggac
      601 tcagctgctg aagattttat gaagtctcac gtggaagcaa caattcgtga tgttccttgc
      661 ctgaaaaaac tttctccctg gctgggccgg aaacaccggg ataatacgct cacactgaag
      721 cgtttttcct ctggtgtggg gttctggtgt ttgggtggtg ctgctgccaa aaactaccgt
      781 gaaaaatctg tcgatgtggt ctgctatgac gaactctcct cgtttgaacc ggatgtggaa
      841 aaagaaggtt cgccaacact gcttggcgat aaacgtattg aaggttcggt atggccaaaa
      901 tccatacgcg gttcaacgcc aaaaattaaa ggctcctgtc agattgaaaa agctgcgaat
      961 gaatctgcgc atttcatgcg gttttatgtc ccttgccctc attgcgggga ggagcagtat
     1021 ctgaagtttg gcgatgatgc gacgtcgttt ggcctgaaat gggagaaggg taaaccggaa
     1081 acggtgtatt acctgtgtga acataatggc tgcgtgatcc gtcagtctga acttgatcag
     1141 accggggggc gctggatttg tgacaatacc gggatgtgga cgcgtgacgg tctgacgttt
     1201 ttcagtgctt cgggtaatga aattccgccg ccgcgctcca tcactttcca tatatggacg
     1261 gcgtacagtc cgttcaccac ctgggtacag atagtctgtg actggctgga tgcactgaaa
     1321 gatcccaacg gcgtgaaaac ttttgtgaac accacgctgg gcgagacctg ggaagaggcc
     1381 gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcctta cacggcaacg
     1441 gtgcctgtcc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
     1501 atgtatgtct gggggtgggc tccgggagag gaagcctttc tggtggataa aatcatcatc
     1561 atgggacgtc ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caataaaaaa
     1621 taccgccatg cagacggtac cgaaatgacc atttcccgtg tctgctggga tatcgggggg
     1681 atcgatggcg aaatcgtgta tcagaggtca aaaaaacacg gtgttttccg ggtactgccg
     1741 gtaaaaggtg cctctgtcta tggcaagccg gtgatcacca tgccgaaaac ccgcaatcag
     1801 cggggtgtgt atctgtgtga ggtggggacg gataccgcaa aagaaattct ctatgcccga
     1861 atgaaggccg atcccacccc tgtgaatgag gccacatcgt atgccatccg ctttcctgat
     1921 aatccggaga ttttttcgca gacagaggca caacaactgg tggcggagga actggtggaa
     1981 aagtgggaaa aaggaaagat gcgtctgttg tgggataaca aaaagcggcg taacgaagcg
     2041 ctggactgcc tggtgtatgc ctatgcggca ttacgtgtgt ccgtgcaacg ctggcaactt
     2101 gatctggctg tgctggcggc atcccgcaag gatgcaactg cacaaccgac ccttgaagaa
     2161 ctggcagcga agctgtccgg aggagtgaat ggttacagtc gctgaactgc aggcgctgcg
     2221 tcaggcgcgc cttgatttat taaccggtaa acgggttgtg tcggtacaga aagacggtcg
     2281 caggatcgaa tatacggcag cgtccctgaa tgagcttaac cgtgcgatca acgatgcgga
     2341 gtcggttctg ggtacgaccc gacgccgccg accgctggga gtgaggttat gaaacgaacg
     2401 cctgtcctga ttgatgtgaa cggcgttccg cttcgtgaga gtctcagcta caacggaggc
     2461 ggcgcaggat ttggcgggca aatggcagag tggttaccac cggcgcagag tgccgatgcg
     2521 gccctgttgc ctgcattgcg tctggggaat gcccgggcag atgatctggt gcgcaataac
     2581 ggtatcgcgg ctaatgcggt ggccctgcat aaggatcata tcgtcgggca catgtttctt
     2641 atcagctacc gtctgaactg gcgttggctg gggatgcggg agaccacggc aaaaagtttt
     2701 gtcggggagg tggaagcagc ctggtcggaa tacgcagaag gaatgtttgg cgagatcgac
     2761 gtggaaggga aacgcacgtt tacggaattt atccgtgaag gggtgggcgt gcatgccttt
     2821 aacggcgaaa tctttgtgca gccggtctgg gatacggaaa ccacgcagtt attccgtacg
     2881 cgttttaaag ccgtgagtcc gaaacgggtg gacacgccag gacacggtat ggggaaccac
     2941 tttctgcggg ccggggttga ggtcgatcgg tatgggcggg cgattgcgta ccatatctgt
     3001 gaggatgatt ttccgttctc tggtgccgga cgctgggagc ggatcccgcg tgagcttccc
     3061 accgggcgtc cggccatgct gcatattttt gagccggtgg aggacgggca gacccgtggg
     3121 gctaatcagt tttacagcgt aatggagcgg atgaagatgc tggactccct gcaggccacg
     3181 cagcttcagt cggccattgt gaaggcgatg tatgcagcca caatcgaaag tgaacttgat
     3241 accgaaaagg catttgaata tatcgcgggg gcaccgcaag gtcagcagga taatccgctt
     3301 attaatattc tggagaagtt cacccgctgg tatgacacga ataacgtgac gctgggcggt
     3361 gttaaaattc cgcacctttt ttcctggcga tgcgctgaac ctgcagacag cgcaggattc
     3421 agacaatgga ttttcggcgc ttgaacaggc gctgctgcgg tatattgcag ccggtcttgg
     3481 cgtttcctac gaacagttgt cccgtgatta ctcgaaggtc agttactcaa gtgcccgcgc
     3541 ctccgccaat gagtcgtggc gctattttat ggggcggcga aaatttattg cggcccggct
     3601 ggccacgcag atgttttcct gctggctgga agaggcgctt cttcggggga ttattcgtcc
     3661 gccacgggcg cgttttgatt tttaccaggc gcgttcagcc tggtcacggg cagagtggat
     3721 tggctccgga agaatggcca ttgacgggct caaggaggtc caggaatcgg tgatgcgcat
     3781 tgaggccgga ctgagcacgt atgagaaaga actggcactg atgggcgaag attatcagga
     3841 cattttccgc cagcaggtca gggaatccgc agagcgcgaa aaagccggac tctcacgtcc
     3901 ggtgtggata gcccaggcgt atcagcagca gatagcggag agtcgcaggc cggaagagga
     3961 gacaacatca cgtgagacgt aatctttcac atattatcgc tgcggcattc aatgagccgc
     4021 tgcttctgga gcccgcctat gcgcgggttt tcttttgcgc gctcgggcgt gagatgggcg
     4081 cagcaagcct ttcggtaccg caacagcagg tacagcttga cgctcccggg atgctggctg
     4141 aaacggacga gtacatggcc ggaggcaaac gacctgcccg tgtttaccgg gtggtgaacg
     4201 gtattgctgt actgccggtg accggcacgc tggtacaccg gctgggtggc atgcggccat
     4261 tttccggaat gactggctat gacggcattg tcgcctgtct tcagcaggca atggcggaca
     4321 ctcaggtacg gggcgtactg ctggacattg acagtcctga cgggcaggcc accggcgcgt
     4381 ttgactgcgc tgacatgatt taccgtctcc ggcagctgaa gcctgtctgg gcactgtgca
     4441 atgacacggc ctgttcagcc gccatgttac tggcatcggc ctgctcccga cggctggtta
     4501 cccagacatc ccgtatcggc tccattgggg tgatgatggg ccatgtcagc tatgccggtc
     4561 atctggcaca ggccggtgtg gatatcacgc tgatttacgc cggatcgcac aaggtggatg
     4621 gcaatcagtt tgaatccctg ccagcaaagg ttcgccagga catgcagcag cgcattgatg
     4681 cggcgcgccg gatgtttgct gaaaaagtgg cgatgtatac cggtttatct gtggatgcgg
     4741 ttatgggaac agaggccgcc gtttttgaag gtcagtccgg tattgaggcc gaactggcgg
     4801 atgaattaat caatgcgtcg gacgccatca gcgtgatggc tgcgacgctg aacacacacg
     4861 atacaggagg cactatgccg caattaactg caacggaggc tgccgcgcag gagaaccagc
     4921 gagtgatggg gatcctgacg tgtcaggaag cgaaaggacg tgaacaactg gccacgatgc
     4981 tggcagggca acagggtatg agcgttgagc aggcccgggc gattctggcc gtggcggcac
     5041 cgcaacaatc tggtgtatct gcgcagagtg aagccgatcg cattatggca tgtgaagagg
     5101 cgaaaggacg tgaacaactg gcggcaacgc tggcggcgat gccggagatg acggtggaaa
     5161 aagcccgtcc gattctggct gcttcaccgc aggcgaatgc cggaccctca cttcgtgatc
     5221 agattatggc cctggatgag gcaaaagggg cagaggcgca ggctgaaaaa ctggcggcct
     5281 gtccggggat gaccgtggag aatgcccggg ctgtactggc ggcagcctca gacagggcag
     5341 aaccggtttc tgcatccaca accgccatgt ttgaacattt catggcgaac cattcaccgg
     5401 cagcggtaca gggtggcgtg ccacagacgt cagcagacgg tgatgcggac gtgaaaatgc
     5461 tcatggccat gccatgaagt cagtgctgac catcaatatg aggtttttac aatatggtaa
     5521 cgaaaaccat cactgaacag cgtgcggaag tacgtatttt tgccggtaat gatccggccc
     5581 ataccgccac aggcagcagc gggatttcct cggcaacacc ggctctgacg cccctgatgc
     5641 tggatgagga taccgggaaa ctggtggtct gggacggaca gaaggcaggc catgtggttg
     5701 gcatactggt actgccgctt gaaggtacag agacggtgct gacgtattac aagtcgggga
     5761 cctttgcgac ggaggccatc cactggcctg agagagtgga tacacacaaa aaggcgaatg
     5821 cctttgccgg cagtgccctg agtcacgcag cgctgccgta acattttatc aggtcactgc
     5881 ggtagcctga ctgatttctg aatgaaagga actgatttat gggattgttt acgacccgcc
     5941 agttactcgg ttataccgaa caaaaagtta aatttcgtgc gctgtttctg gagctgtttt
     6001 tccgccgtac ggtgaatttc cataccgaag aggtaatgct ggacaaaatt accggaaaaa
     6061 cgccggtggc ggcctatgtc tccccggttg ttgaaggaaa ggtgttgcgt caccgtggcg
     6121 gtgaaactcg tgtactgcgc ccgggctacg ttaagccgaa acacgaattt aattaccagc
     6181 aggcggtgga gcgtcttccc ggtgaagatc cggcccagct gaacgacccg gcttaccgtc
     6241 gcctgcgtat catcaccgat aacctcaaac aggaagagca cgccattgtc caggtggaag
     6301 agatgcaggc ggtgaatgcc gtgctgtatg gcaaatacac catggaagga gagcagtttg
     6361 aaactgttga ggtcgatttt ggccggtcgg tgggaaataa cattacgcag gcaggaggga
     6421 cggagtggag cgctcaggac cgcgatacct ttgatccgac gcatgatctg gatgcgtact
     6481 gtgattttgc ttccggcacc atcaatattg ccattatgga tggtaaggtc tggcgtctgc
     6541 tgaatggctt taagctgttc cgcgaaaaac tggatacccg tcgtggctca aattcacagc
     6601 tggaaacggc agtgaaagac ctgggggcgg tggtgtcctt caaagggtat tatggtgatc
     6661 tggcaatcgt ggtggcaaaa acgtcctatg tggcagagga cggtaccgaa aaacgttatc
     6721 tgccggacgg cacactggta ctgggaaata cggccgcaga gggtattcgt tgctacggtg
     6781 ccattcagga tgcgcaggca ttgtctgaag gcgtggtggc gtcttcccgt tatccgaaac
     6841 actggctgac cgtgggtgat ccggcgcgtg aattcaccat gacgcagtcc gcgccgctga
     6901 tggtgctgcc ggatccggat gagtttgtgg tggtgcaggt gaaataatcc gtgagcggga
     6961 gctaaatgct cccgtttttc attcaatcag gagagggtat ggctacgaaa gaagaaaacc
     7021 tgaaacgtct ttgtgagctg gctgagaggc tggggcgtga accggatgtc tccggtagtg
     7081 cggcagatat tgcgcagcga gtggcggaac tggaggaaga gcttggagat gcgggggagg
     7141 tcgcagagcc ggatacgtac tcaccatcga aagatgatcc gaccgcgcat aaaggcgagt
     7201 cagttcctga acagccaggc attgttaccg gagacgaaac ggggcaggtt acggtggtgg
     7261 cactggcgac actgcataca aaatgtaatg gttcagtgat atttgtttca ccgggaacgt
     7321 cgtttcgtgt ttctgctgag gtggctgcca gcatggcggc aggtggtctg gcaaaaaggc
     7381 agtaacagga gatgtcgtgg acggtttcga taatctgttt gatgctgcgc ttgccggtgt
     7441 caatgaagtc attctccgtg atatggggat cagcgccgtc attacgtccg gagaacttga
     7501 ggggacacac ctgacagggg tttttgatga tcccgaaagt atttcttttg ttgccggggg
     7561 gatccggctt gaggattcct caccgtgcct gtttgtgaaa acggcagata tatcgcagtt
     7621 acgccgtcag gatacgttaa ctatcgggga tgattctttc tttgtggatc gtattactcc
     7681 ggatgacggg ggatgctgtt atatccggtt aaggcggggc agtcctgccc cacagaataa
     7741 tgccagaatg aggcgatatg atgaagggac ttgagaatgc catccgcaat ctgaacagcc
     7801 tcgatacccg catggtgcca cagaccagcg catgggcggt aaaccgtgtg gcggcaaaga
     7861 ttgtttctgt cgccacacgg caggttgcgc agaataccgt tgccggggat aaccaggtga
     7921 aaggtattcc cctgaaaacg gtcagggagc gtgtcaggct gcttaaagcc agcccctcag
     7981 gaaaaatgta tgcccgcatg cgtgttaacc ggggtaacct gcccgccatt aagctgggta
     8041 ccgcacaggt cagactggcc cgctcccggc atggcagcaa ctcacgtcat cgtggcagcg
     8101 tactgaaagt ggggaaatac cttttccggg atgcgtttat tcagcaactg gcgaatggtc
     8161 gctggcatgt gatgcggcgt attgacggca aaaatcgtta ccccattgat gtggtgaaaa
     8221 tcccg
//
LOCUS       DAOFMX010000142         7884 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.143, whole genome shotgun sequence.
ACCESSION   DAOFMX010000142 DAOFMX010000000
VERSION     DAOFMX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.143"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(1..111)
                     /locus_tag="QBA20_003932"
                     /pseudo
     CDS             complement(1..111)
                     /locus_tag="QBA20_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            109..433
                     /locus_tag="QBA20_003933"
                     /pseudo
     CDS             109..433
                     /locus_tag="QBA20_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(463..3375)
                     /locus_tag="QBA20_003934"
     CDS             complement(463..3375)
                     /locus_tag="QBA20_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294283.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3378..3494
                     /locus_tag="QBA20_003935"
     CDS             3378..3494
                     /locus_tag="QBA20_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDQ6294284.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3858..4700
                     /locus_tag="QBA20_003936"
     CDS             3858..4700
                     /locus_tag="QBA20_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDQ6294285.1"
                     /translation="MLNEGGILFKANHIPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4703..5791
                     /gene="capU"
                     /locus_tag="QBA20_003937"
     CDS             4703..5791
                     /gene="capU"
                     /locus_tag="QBA20_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HDQ6294286.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5796..6746
                     /gene="virK"
                     /locus_tag="QBA20_003938"
     CDS             5796..6746
                     /gene="virK"
                     /locus_tag="QBA20_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HDQ6294287.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6811..7755
                     /gene="lpxM"
                     /locus_tag="QBA20_003939"
                     /gene_synonym="msbB"
     CDS             6811..7755
                     /gene="lpxM"
                     /locus_tag="QBA20_003939"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDQ6294288.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2126 a         1767 c         1865 g         2126 t
ORIGIN      
        1 acttccgtaa agaagggcat caagctcctg ctccgacata cctgcaggca aaggccatga
       61 aagccctgat ttccggaagc gagcgaacag tccacaaact gttgaacgtg gtgtcagcgg
      121 cggacaggga taagccctcg tcagcagttc cgtcagcact gcgacagcgt tctttacccg
      181 gtcaaaacag cgttgcggtg caccacgcct gacggatgaa ctgcgtgctc agggttacca
      241 ctttaacgta aaaaccgtgg cggcaagcct gcgccgtcag gggctgaggg caaaggcctc
      301 ccggaagttc agcccggtca gttaccgcgc acatggcctg cccgtgtcag aaaatctgct
      361 ggagcaggat ttttacgcca gtggtccgaa ccagaagtgg gcgggagaca tcacgtactt
      421 acgtacagat gaagttcggt tacatcctgt cagcacggaa cctcacgctt tttgagcggc
      481 acgacggatc ccccccaccc agtatccgac agacttttca ctgcgttcgt aaaaaccctt
      541 cttccctccc agcatatcca ctaattccgg tgacatatcc tgcagataag tctgcaaatc
      601 atgccccaga tcttccggtg actgcaactc ataaaccgcg cgccacacat cacccttcct
      661 tattttaagc cccgggttaa gaatatactg tccgtggttc agcctcagaa aaagcttttt
      721 tccataagga tttttgctgt taacttcatg ttgagagagc tggctgctga tataactcct
      781 tcgtttgcgg cgttccggca gcaacgcttc aggaagatga accacacact ctgccagtcg
      841 tgccgcactg aaggcttccg aaaaaaacag agcacttccc agatgcgtgt ggaacagcgc
      901 ctgcatcagg atcaccagaa acattgccat ctgccggttg tccagtttga tgaggcgtcc
      961 ttccacctgc agtgacagac tttcaggttt cagcagggta tacaactgcg cacttttctg
     1021 ttgcgcataa cggggcgtac tgaccgccgc tgacagaact tgatgataag ccgccagccc
     1081 gtcattaccg gtcagcaagg gatctgcacc acgctccagc agtaactgaa ccagtgcaat
     1141 attacctgcc cgggctgctg acatcagcgg agtaagatta aacggcgaac ggtgatcaac
     1201 accataacgt tcaatatccc gcaacacggc aaccgggtta ttcattgagt atggccggaa
     1261 atacttcagc tccagtaact gcagagtctt cgttctgggc tgccttgtgg gcttaaaatc
     1321 ttcccggtac agtgcattat acagcgggaa cagggaataa atcagactgt actccagcgc
     1381 ctgcagttgt atggttttat cggctgtacc tgcgaggata tgctgtttcc actgtcgtgc
     1441 atcctctgcg gtgataatct gccatggcat ctcagatgtc tgcaggatcc ggctacggat
     1501 ggtgtccgcc tgctcctgct tcccctgttt ttcaagcaga tgtgcggttt tctgccactc
     1561 atcacggctg gaaatctctt cctgcagatt cagaggctct tcctgatgcg tgagcgcaag
     1621 cagactccac agaggatgct ccacctgctg ttcaacaaga tacacattgt gtgtagcacg
     1681 ggtgagcgca acatacagcg cgttggtaaa aaatttatag atttccgctg aacgatcctg
     1741 cctgtcccgc ggacgggaat aacgggcctc gccctcaaga tcagccggtg taagaccctc
     1801 gcaaatatca tcaaatgcct gccgggcagc gcttactata ttgtaaagga tcaccgtttc
     1861 gtattccagc cccttaacct cctgaaccga aaagaccagc ggcgtactga accagcatcg
     1921 cgcctgtgct ttctgttccg gatgcatcac aatgaccgct acacgattcg aaagtgaggt
     1981 tttggcattc agttcctgct ttgtctcttc cctatcgtcc agcaaccgga tggtcccttc
     2041 tgcctgaccg cagcttctga caaaatggtg actttcccga tcaatagcac taaagcggac
     2101 ctgtttgagt ctcagcaacc ggttagccag tgcagtgacg tggtgcccgt tacggtaatt
     2161 cgcctgcagg atattaacgg ttgtgtcgtt tccctgttgc tgccggaaaa agagactttt
     2221 gagcgaactc catgagaaaa agctcggatg aactatctga ttggcatcac cacagagtaa
     2281 aaactgaccg ggatgacgta gcgttttcag taccagttgc agttgaacag gcgtcatatc
     2341 ctgaacttca tcaacaaaaa tgacatcata acggggagtt gccaacgaaa gatactgatg
     2401 actgagcaga ttactgtcat actgatgcga ctgtttcagc catgcgatat aacgttcaaa
     2461 caggacatag accgtcgggc ggtcctccat gccatagagc gactgccgta tccccagtga
     2521 cagatacgcc tcccgggaca gcggcccatt cccggaggca accgcaccga tcacgcccct
     2581 gaactcctca tacagggtat gggcagcccc cagtgcggcg atagccctgt tgcggggcag
     2641 ccagtcgctg aacgcactga aagtcacctc cctgccttcc ggaatgcgca gagtttccag
     2701 aaattccgtc agcgaaagaa agtcaatatt ctgtacctca ctcccttccc ctgaagcgtc
     2761 atagagagta cgcgcttttt ccaccagaaa tgaggaaagg ctgagataca gtatatcacc
     2821 agcagcctgc ttcatttttt ccagtaccag cgccgtctta ccacttcccg ccgagccaac
     2881 aatcaccagc ggtagcgggt gttcgtacag tgcctgctga tcatcatcaa aggacagcat
     2941 tttatccagg cggtggaaac gcccatgaga cggattgata tacgtcagcg cttcagtcgc
     3001 gatatcaaca ggatccggca ctggttgttg ttgtaagcgc ccttcatcaa tcaccacgtt
     3061 tctgcgaaga aaacgtgaag tgttataggc atgattgcga atatattcca gaacaaggca
     3121 gatagtttca ccgtggtagc gatacagcga aaacagtaat cggtcccgga tactgagccg
     3181 ggcgcggtaa agattggtgt caattttacg aacatcagcc tgaacaaagt tatcggtggc
     3241 aatagcctgt gcaaattttt tataacccgg gatcgtgtca ggattcagag tatgataaag
     3301 acagattttc atggctgccg gtttccgtcg tgcaaaaaac atgctgattt tacgaaggaa
     3361 tgccttactg aacaaccttg cccggaatac cccccgaaat cagatggaac aatcagaaaa
     3421 atgctctgca gttatggaat aaagaaggtg gggtggtacg ccgggagggc gatccgctgg
     3481 aaatggcgca gtagctgcag gcctgtcatg atgccggaat agaagttcgt gtacaggtta
     3541 atgaaagcgt caccccgtaa ataaactctg cgctgaaatt cctttttttc attggagcac
     3601 ctgtgttgtt ctgaggtgaa catatcacct ctgttcaggt agccaaattc agtgtgccac
     3661 ctcaaggttc ttagtgagtc gtttttaaaa gatatgataa tatatcattg tttatgaatt
     3721 gttgatgttt aatttagtaa attatatcaa atatgccatg ggtttgctat gttattcatc
     3781 cagtatctgg aaataaaatg caccagcttt attgggtttt ccttttgtct ctgcttacta
     3841 ggtgccagaa actttaaatg ttaaacgaag ggggcatttt gttcaaagcc aatcatattc
     3901 cggtattaat gtatcatcat gtcagtcatt gccccggact ggttacactt tctcccgtga
     3961 cattccggaa gcaaataaaa tggttggctg aaaacaactg gaaaacactg tcatcagatg
     4021 aactggagtt tttttatcgg ggcggaaaac tcccccggaa aagcgttatg ttgacatttg
     4081 atgatggata ccttgacaac tggtttcagg tatatccttt attaaaagag tttaatctca
     4141 aagcacatat ttttttaatt accggtttta ttgggaatgg tccggttcgt cactctcctg
     4201 gaaaggaata ttctcatcgc gattgcgaac atcaaatagc gacagggaat gctgataatg
     4261 ttatgcttcg ctggtctgaa gtaaacgaaa tgttgcaatc tggtctggtc gaatttcatg
     4321 ttcatacgca tactcataca cgctgggata aaaagttcag ttcacgagaa gaacagtgta
     4381 aacatcttcg gcaggacttg ttgagcggga gagaatattt gaaagaaatg acaggaaaat
     4441 gcagtaaaca tttgtgttgg ccggaaggtt attataacaa ggattatatt caggtcgctg
     4501 aagaacttgg attctattat ttatatacaa cagaaagaag aatgaatgct cctgctaaag
     4561 gaactacacg gataggaaga ataagcacca aagaacggga aagttgcgca tggctgaaaa
     4621 gacgactttt ttattataca accccgtttt tttcatcgtt gctggccttt cataaggggc
     4681 caagattacc ggatgattaa taatgaatat actatttacg gaatcatcac cgaatatagg
     4741 tggtcaggaa ttacaggctg ttgctcaaat gaaggccctg aagaaaatgg ggcattcagt
     4801 tctgcttgtc tgcagggaaa acagcaaaat tgcttttgaa gccagtaaat tggggattga
     4861 tatcacattc gcgttatttc gaaacagtct tcacatccct actgcatgga gattactcgg
     4921 aatagttcat ggttttcagc ccaatgcaat cgtttgtcac agtgggcatg atagcaatat
     4981 tgttggttta gtacggttat tcactaggaa acatccattc agaattatca ggcaaaagac
     5041 atatttgaca cgaaaaacaa aagttttttc aataaatcat ttttgcgatg aggtgattgt
     5101 tcccggaaca agtatgaaga cacatctgga gcaggaagga tgtcgaaccc gggttactgt
     5161 tgtgcctcca ggctttgatt tccagaaatt atacgttgat tcccgaaaca gtttgcctcc
     5221 aaatgttctt tcttggctgg cgtcccgaag gggatgcccc gttattgctc aggtaggaat
     5281 gttgcgcccg gaaaaagggc acgaatttat gttgaattta ctgttccatt taaaaatgaa
     5341 tggacgacag ttctgttggt tgattgtggg gtctggttcg cctgaactgc gggagcattt
     5401 acagtatcag attgacagta tgggcatgca tgatgatgtt tttattgctg acaatgtttt
     5461 tcctgccgcc cccgtatatc gggttgccag tctggtggtt ctgccttcag aaaacgaatc
     5521 gtttggtatg gtactggcag aagcatcggc attttctgtg cctgtactgg ccagtcagat
     5581 tggtggaatc cctgatgtta ttcagaataa ccagaccggg acattgttac cagcaggtaa
     5641 taagcacgca tggatgtgcg ccctgaatga tttttttaat gaccctgggc gtttttatca
     5701 gatggctcgc caggcaaaac aggatataga agagcggttt gatattaata aaactgcgtt
     5761 aaaaatactc acattagcga agcacaagta acaatatgtt ttctgtaagt aacttatcat
     5821 ttatcggttt ccttaaaagg attgtttttt cctcagattc actcccgggg aagtgggaac
     5881 acagaaaatt ccggttcatg tacattttgc gatgtgctat aaatccggtt gccagtattc
     5941 gatattatta cgaactgcgt tccttgcagt gcattgagga tattctggca atacaaccca
     6001 cgttgccagc aagaattcat cgaccttatt tgcataaagg ggggcgagcc tggtcccggg
     6061 ggcaatatat tcttgagcat tatcgctttg tgcaaaattt gccagaaaaa tattctgaat
     6121 ttttgtttcc acaaaaatcg gtatcattag tccaattcat cgggaaagat ggagaggatt
     6181 ttgatatcca gtgttctccc agtggttttg accgggaagg agagttgatg ttgtcattgt
     6241 ttttcaataa aatagtgatt gcccggttaa cattttcagt cattctgact caaaatggac
     6301 acaccgcttt tattggcgga ttacagggag cccccaaaaa taccggacct gatgttattc
     6361 ggtgcgcaac ccgggcctgt tatgggcttt tcccaaaacg gattattttt gaagcatttt
     6421 gtgcgttgat gaaagcttgt aatgtctctg agtgtctggc agtcagtgag cacagtcatg
     6481 ttttccggca attgagatac tggtatcaga aacgcaaaac ctttgttgct gtctacagtg
     6541 atttctggga gtctgtagca gggaaaacct gtggtgactg gtacaaatta ccaacacagg
     6601 tggttcgaaa gccgctaagc aatatagcta gtaaaaaacg ctctgagtat cgaaaacgat
     6661 acgctttgct ggattatatc catgaaactg ctatccgctc tttggatgca tatcctgtaa
     6721 actcagaaca tcaggactta aattaaggtt tgatgtattc tctgaataaa atattaatga
     6781 tgattttggt aggggcattc gcactaaata atgaaaaaat acaaatccga gtttattcct
     6841 gaatttaaga aaaattatct ttcccctgtt tactggttta catggttcgt tttgggaatg
     6901 attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc caaaataggg
     6961 cgttgggtgg gcagattgag cagaaaagct cgtcgcaggg cgacgattaa tttatcgctt
     7021 tgtttcccgg aaaagagtga tacagaacgg gaaataattg tcgacaatat gtttgccaca
     7081 gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtccgga aaagttccag
     7141 aaacgagtgt tctggaaagg cctcgaaatt cttgaggaaa ttcggcacaa taacagaaat
     7201 gtgatttttc tggttcccca tggctggagc gtggatattc ctgcaatgtt gctggcagct
     7261 cagggggaaa aaatggccgc catgtttcat cagcaacgaa atccagtgat tgattatgtc
     7321 tggaattctg tacggcgtaa attcgggggg cgcttacatt cccgggagga tgggataaaa
     7381 ccatttattc agtcagtacg ccagggatac tgggggtatt accttccaga tcaggatcat
     7441 ggtcctgaat acagtgaatt tgctgatttt tttgcgacct ataaagcgac attaccaatt
     7501 attggacgtc tgatgaacat cagtcaggct atgattatac cgcttttccc ggtttatgat
     7561 gaaaaaaaac atttcctgac tattgaagtt cggccaccaa tggatgcatg cattgccagc
     7621 gcggacaata aaatgattgc ccgacaaatg aacaaaacag tggaaatttt ggtggggtca
     7681 catccggaac agtatatctg ggttttaaaa ttgttaaaaa cgcgcaaatc aaacgaagcg
     7741 gacccgtacc cttgaaatta taaagtacag gtatttccac tagttgtttc ttacaggtta
     7801 ccaatcgaaa cacatccccc ttccgtgtgt tgcccgtttg gcacggagga ctgtgtagat
     7861 gcagttcgtt aaatttaatt tatg
//
LOCUS       DAOFMX010000143         7791 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.144, whole genome shotgun sequence.
ACCESSION   DAOFMX010000143 DAOFMX010000000
VERSION     DAOFMX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.144"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..1332
                     /gene="yjeM"
                     /locus_tag="QBA20_003940"
                     /pseudo
     CDS             1..1332
                     /gene="yjeM"
                     /locus_tag="QBA20_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="QBA20_003941"
     CDS             complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="QBA20_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDQ6294289.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4816..5784)
                     /gene="psd"
                     /locus_tag="QBA20_003942"
     CDS             complement(4816..5784)
                     /gene="psd"
                     /locus_tag="QBA20_003942"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDQ6294290.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="QBA20_003943"
     CDS             complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="QBA20_003943"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDQ6294291.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7028..7573
                     /gene="orn"
                     /locus_tag="QBA20_003944"
     CDS             7028..7573
                     /gene="orn"
                     /locus_tag="QBA20_003944"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDQ6294292.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1763 a         2024 c         2108 g         1896 t
ORIGIN      
        1 cttaatgatg gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta tctattcctg
       61 gatgaataat agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc
      121 ttatatcatc tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct
      181 ctatggtagt gacatgaccc agcactggcg tattgccgga ctggagccta cgcaggtggt
      241 tggtctgctg gcagtagcat ggatgattct ggtcaccgtc gttgcttcaa aggggattaa
      301 taaaattgcc cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt
      361 gctgttagta agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa
      421 tttccttgca tcaccgaacc cgggttatca gtccggtctg gcaatgctat cgtttgtggt
      481 atttgctatt tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga
      541 aaatccagaa aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg
      601 ttattcgctg gcaatatttt tatggggcgt cagcacaaac tggcagcagg tattaagtaa
      661 tggttccgtt aaccttggca atattaccta tgtgctgatg aagagcctcg gggtgacgct
      721 gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat ggtttgcgcg
      781 tattaccgga ctttcgatgt tcctcgctta taccggtgcg tttgctattc accgctgaaa
      841 gccatcatcc aggggacgcc gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg
      901 atggggatgc cgtctatcgc catgtggatg cagtgcgggt tggttactgt cttcattctg
      961 ctggtttcgt ttggtggcgg taccgcatcg gcgttcttta acaagctgac gctgatggcg
     1021 aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg ctttcccgtt ttttaaagca
     1081 cgtcaggatc tcgacagacc atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg
     1141 acggtggttg tcgttctggt ggtgacattt gcgaacgtct tcaccattat tcagcctgtg
     1201 gttgaagccg gagactggga cagcacattg tggatgattg gcggccctgt cttcttctcg
     1261 ctgttagcga tggcgattta ccagaactat tgcagtcgca tggcgaataa acctgagtta
     1321 gctctcgact gatcatatga aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag
     1381 gaagtaatgc gtatggatga tactctccag ggccggtgat aactgaagat gatgttaagc
     1441 tgctaaatga gccacgttga catcatttgg cagcttcatg cattacaaac ttcccgcctg
     1501 acgacctttg cccgcagacg tcagcgttct ccccgtttgt ttaccgttga ggctttccag
     1561 acgcatctgg aagggcggga atggcatatc gataccgtgg gcgtggaagc ctgccagaat
     1621 cagctggtgg atctcatggc gtagcggcat acggtgaccc atctcagcgg cgtaaatacg
     1681 cagctcgaaa atctgaatcc cctgttgcag atccaccagg aagacttccg gtgccgggtt
     1741 gtcgatcacc agcgagcagc gacgcgctgc ggtgagcagg atttccgtca cttcttcact
     1801 gttggcatcg gcaggggccg gaatcgtcaa cactacgcgc gtgaccgagt cagagagcga
     1861 ccagttgata aactgctcgg taataaacgc cttgttcggc acgattatct ctttacggtc
     1921 ccagtcgctg atggtggtgg cgcgggtgtt aatttttgtc acgctaccag taagatcgcg
     1981 gatcgttacc gtatcgccaa tgcgaatcgg tttttcgaac aggatgatca ggccagagat
     2041 aaagttggcg aaaatttcct gcaaaccaaa accgagacca acaccgagcg cggcaaccag
     2101 ccactgcaat ttcgaccact caataccaat cattgagaag ccgaccagcc cgccaatcag
     2161 catcagcaga tattttgtga tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag
     2221 gtgctgcaaa atcgccagtt ccagcagcgc gggcaggttg cgcaccagct gcgtggtgat
     2281 gataaatacc agaatggcaa tcagcaccgc accgagggta attggctcca gactttctac
     2341 gccctgtacc gtggaggtga catcccacag cgaaatattt tcgaggaagc cgaaagcgga
     2401 atggatttct gaccacagca caatgaccga aagcagggcg atcaacatca aaattgagcg
     2461 caccagccgc aaggattgcg cactgatggc atcgagatcg acttcgcttt catcgacttc
     2521 aattgctcct tccgggctac tgtgatgatg cgcttcctct tcgccacgcg cacgttgcgc
     2581 taacatctct gcccggcgat gcttcgcccg gtcaaacgcc agcctgcgac gctggatcag
     2641 catccagcgg cggataacgt gataaaccac cagtagcagg aaccagatgg caaccgaggt
     2701 ttcaagcctc gctaacagtg cctgcgccgt tgccagataa cccaccgccg acgccagaat
     2761 ggcaaccagt ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa tgttgtcgcc
     2821 gctgccttct ttgttgagat acaacggaat accggctttt ttcaggctta gggtgaccac
     2881 cgccagcgca ccgcaaatga ggataaagca aagccgtccc agcgaaccgg aaaattcgcg
     2941 gtcgtcgagg ttatcgaaca tcatcagcgc cataatcagc ccgatgctca tcaggtagta
     3001 gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt
     3061 cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg tggccgttac
     3121 accatcgcca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc cgtaacccag
     3181 cgtcatccac agcaccggta acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa
     3241 cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa
     3301 acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaaacag
     3361 cggcaaaatt gtctctttgc tggtcagcat catcacactg gctttgccca actgactgaa
     3421 ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagcggcc aggcgatggt
     3481 catcgggcgc acgtcagagg cccagaacag atagcggtgc gttgcttcgt tcacctcttt
     3541 cagcgcatcc tccagttgcc cgttggagac tttcagcttg gtcagttcca atagtagcgt
     3601 gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag
     3661 aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatggattt gccgtagcag
     3721 cggctgttta ttaagtaaat cctcataacg taaccgttgc acacgcaact gcgccatttc
     3781 ggtatcaagc tgttgtggtt ttggcatttc tggtagccgt gccacctgcg cccgcagcgc
     3841 ttcgccgagc aggttggacg atcccagcca ttgcgactgt tcacgcagcg tattcaacgc
     3901 ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt tgcgaggcaa cgagctccat
     3961 ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg ttaattttga attgcgcgac
     4021 gatatctttc ggcaaatcgg cactgttttc tgccagtaat tcggtacttt ccagcgcccg
     4081 ctccgcctca agctgacgtt ggctattcaa ctgattacgt aaggcctgca aatacgcatc
     4141 cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt
     4201 attggcagac agctgcgcca gctccagttc atcaacgagc gccttaagac gtgcagagtc
     4261 agactgcaac gcgaaatttt gtgcctgatt gagcggagta ttgccggtaa gcgttcccag
     4321 gcggcgctcg atctcattta actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt
     4381 cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc
     4441 cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acattcccgg
     4501 cgacacgctg cgcggctcgt cacgaatgtt gtttaattgt gcgcgcagag tagcggagag
     4561 tttcggataa ttatcgataa cttgctgata ttgtttgatg cgctcaaggg aaccttttcg
     4621 ttcctcaagc gcatttaagg cagactggag cgcctctacg acttccggct gtgcgggttt
     4681 cgccgctttt gcctgctcca gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc
     4741 gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc
     4801 gtttcctttg atggattaga cctggtcttt tttatcgtca accaatgggc tggcgtcgtg
     4861 ttctgcttcg atctcttcag caggaagtgg ggcgggttca gcgtctggcg taacaaaggt
     4921 ttcggtagat actgccagcg gctggccaat tttcgtgaca gacaggcttt ccagttgctc
     4981 aaccagattc actttacccg gtgcaaacag gttgataacg gtggaaccga gtttaaagcg
     5041 acccatttcc tggcctttca gcagtgccac agaaccgtcg ttttccccgg caggccaggt
     5101 ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat
     5161 gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa
     5221 aaggcaaatc acgcgttcgt tacgggcaaa cagattcggc acgttctgag cggtgagatg
     5281 gttaacggaa aagagatcgc ccggcacgta gatcatctca cgcagaatac cgttgcacgg
     5341 catgtgtacg cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg
     5401 gaacaggtcc gccatcagat agttgcctgc cagcagggct tcgaggctgt agttgtggcc
     5461 tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc
     5521 aggcatgacc agtacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg
     5581 gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat
     5641 gtcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg
     5701 cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag
     5761 cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc
     5821 cggccaacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg
     5881 tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat
     5941 acggtgatag ttttcgaaac gggtttccgc gattttccct tcctcaaccg cttcccggat
     6001 agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaaatagtc
     6061 atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc
     6121 acgcacacca ggggaatcaa tcacatcacc gccgtgcggg aagtgataca gccgagcggc
     6181 ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca tcgttggtca ggatctcttt
     6241 ttgcagcccc agcaacgcgt tcagcaggct ggatttaccg acgccagact gcccggcaaa
     6301 aatgctgatg cgcccggtca acgcctcttc cagcggtttc agcccatcct gagtatggct
     6361 ggaaaccatc aatacgcgat aaccgatgtt gcggtagata tccatctgct cgttgacgaa
     6421 cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc acgataatcg gctcaatctg
     6481 caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat
     6541 ggcggagaca atgacaatct ggtcgatgtt ggcggctatg ggtttcacgc cgtcgtagaa
     6601 atccggacgc gttaacaccg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt
     6661 tacgccttcc gccgccggtt tacccggacg ccagactacg cggtcgccgg ttaccagcga
     6721 acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc
     6781 gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc
     6841 gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg
     6901 ctgctggcct ttggagagtt tatttttact caatcgtact gactcctggt cgcccctgat
     6961 gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga
     7021 aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct
     7081 ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaatctgaa
     7141 tattctggca gaagggccga ccattgcagt acatcagtcc gatgaacagc tggcgctgat
     7201 ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtagagcgcg tgaaagcgag
     7261 cacgatgggc gatcgggaag ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc
     7321 tgcgggaaaa tcgccgattt gcggtaacag catcggacag gaccgtcgtt tcctgtttaa
     7381 atacatgccg gagctggaag tctacttcca ctaccgttat ctcgatgtca gcaccctgaa
     7441 agagctggcg cgccgctgga agccggaaat tctggatggt tttaccaagc aggggacgca
     7501 tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg gcttactacc gcgagcattt
     7561 tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg
     7621 attttgacgc taaatcgaac gtcttgctga taatttcagc acttgagata aaaacgcaaa
     7681 aaaaactttt ttggggggtt gcagagggaa agatttctcg tataatgcgc ctcccgtaac
     7741 gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag tacgcgggaa t
//
LOCUS       DAOFMX010000144         7747 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.145, whole genome shotgun sequence.
ACCESSION   DAOFMX010000144 DAOFMX010000000
VERSION     DAOFMX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.145"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..1016
                     /gene="ydfJ"
                     /locus_tag="QBA20_003945"
     CDS             <1..1016
                     /gene="ydfJ"
                     /locus_tag="QBA20_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HDQ6294293.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMLEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1105..2565
                     /locus_tag="QBA20_003946"
     CDS             1105..2565
                     /locus_tag="QBA20_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDQ6294294.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2601..2804)
                     /gene="ydfZ"
                     /locus_tag="QBA20_003947"
     CDS             complement(2601..2804)
                     /gene="ydfZ"
                     /locus_tag="QBA20_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDQ6294295.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2982..3668)
                     /gene="ydfH"
                     /locus_tag="QBA20_003948"
     CDS             complement(2982..3668)
                     /gene="ydfH"
                     /locus_tag="QBA20_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDQ6294296.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDADAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3757..4503)
                     /gene="ydfG"
                     /locus_tag="QBA20_003949"
     CDS             complement(3757..4503)
                     /gene="ydfG"
                     /locus_tag="QBA20_003949"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDQ6294297.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4640..6685
                     /gene="dcp"
                     /locus_tag="QBA20_003950"
     CDS             4640..6685
                     /gene="dcp"
                     /locus_tag="QBA20_003950"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDQ6294298.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6730..7248)
                     /locus_tag="QBA20_003951"
     CDS             complement(6730..7248)
                     /locus_tag="QBA20_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HDQ6294299.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7524..>7747
                     /gene="ydeI"
                     /locus_tag="QBA20_003952"
     CDS             7524..>7747
                     /gene="ydeI"
                     /locus_tag="QBA20_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDQ6294300.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1942 a         1812 c         1957 g         2036 t
ORIGIN      
        1 ccatcttgct ggtgacgctg cgtattattc agggattggg tgcaggtgcg gaaatttccg
       61 gtgccggtac gatgctggcg gaatatgcgc caaaaggtaa gcgcggaatt atctcctcat
      121 ttgtggctat gggaactaac tgcggcacct taagcgcaac ggcaatctgg gcctttatgt
      181 tcttcattct cagtaaagag gaactgctgg cgtggggatg gcgtataccg ttcctggcaa
      241 gcgttgtcgt gatggtcttt gctatctggt tgcgtatgaa tctgaaagaa agcccggtct
      301 ttgagaaggt taacgacagc aaccaaccga cagcaaaacc tgcacctgct ggtagcatgt
      361 tccagagcaa atccttctgg ctggcaacag ggctgcgttt tggtcaggcg ggtaactccg
      421 gtttaattca gactttcctt gcaggctatt tagtacagac gttattgttt aacaaagcaa
      481 ttccaacaga tgcattgatg atcagttcga ttctcggctt tatgaccatt ccgttccttg
      541 gttggttatc cgataaaatt ggtcgccgga tcccgtatat tattatgaat acctctgcga
      601 ttgtgctggc gtggccaatg ctttctatca tcgtagataa aagctatgcc ccgagcacca
      661 ttatggttgc actgattgtg attcataact gtgcggtgct gggattattt gctctggaaa
      721 acattaccat ggcagaaatg ttcggctgta aaaaccgctt cacccggatg gctatttcaa
      781 aagaaattgg tggtcttatc gcttccggtt ttggtcctat cctggcgggt attttctgca
      841 ccatgacgga atcctggtat ccgatcgcca ttatgatcat ggcatattca gtgattggtt
      901 taatctctgc gctgaaaatg ctagaggtga aagaccgtga tttaagtgcg ctggaagacg
      961 ctgcggaaga tcaaccgcgt gttgtaagag ctgcgcaacc ttccagaagt ctgtaaaccc
     1021 tcaatccctt ctcttaccgg agaggggatt tttattcata taacaaaaca tatagcttgc
     1081 catatttaaa tttaaggaat tgttatggga aataatttgt tatcagcaaa agcgacactc
     1141 cctgtttatg atcgtaataa cctggcccca agaattgttc atttaggctt tggtgcattt
     1201 caccgtgcgc atcagggcgt gtatgccgat attcttgcta cagaacattt cagtgactgg
     1261 ggatattatg aggtcaactt aatcggcggc gaacagcaaa ttgccgattt acaacagcaa
     1321 gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt ggacggctcg cgtcgttggc
     1381 gtcgttaaaa aagccttgca cgtacagatg gatggcttag aaaccgtgtt ggctgcgatg
     1441 tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg aaaaagggta tttccactct
     1501 ccggcgaccg gacagttaat gctcgagcac ccgatggtcg ctgccgacgt gcaaaatccc
     1561 caccagccga aaacagcaac aggggtgatt gttgaggcgc tggctcgccg taaagcggca
     1621 ggacttcccg catttaccgt catgtcatgt gacaacatgc cagaaaacgg tcatgttatg
     1681 cgtgacgttg tcacttccta cgcgcaagcc gttgatgtaa aactggcaca atggatcgaa
     1741 gataacgtga ctttcccatc aacaatggtg gaccgtattg tgcccgcagt gacagaggat
     1801 acgctggcga aaatcgaaca acttaccggt gtgcgcgatc ctgctggcgt tgcctgtgaa
     1861 cctttccgcc agtgggttat cgaagataac tttgtagccg gacgtccgga atgggaaaaa
     1921 gcgggagccg aactggttag tgatgtgctg ccttatgaag agatgaagtt gcgcatgctc
     1981 aacggcagtc attcattcct ggcatatctg ggttatcttg ctggatatca gcacattaat
     2041 gactgtatgg aagatgaaca ttatcgtcat gcggcgtatg gcatgatgtt gcaggaacaa
     2101 gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt acgctaaccg attaattgca
     2161 cgctatagca acccggcgtt acgtcatcga acctggcaga ttgcgatgga tggcagccag
     2221 aaattgccac agcggatgtt ggattctgtt cgctggcatc tggcgcatga cagcaagttc
     2281 gatctgctgg cgctgggcgt cgcgggttgg atgcgttatg tcggtggtgt tgatgaacag
     2341 ggaaatccga tagaaatcag tgacccactg ttacctgtta ttcagaaggc tgtacaaagt
     2401 agtgccgaag ggaaagcgcg cgtccagtca ttgctggcga ttaaggcaat ctttggtgat
     2461 gatttgccag acaatagctt gtttactgca aaagtgacgg aagcgtactt gtctttatta
     2521 gcgcatggcg cgaaagcgac cgtggcgaaa tattccgtga agtaaaaaaa gcaatgccgc
     2581 catcggcggc attcacacac ttagttcatg ccgagacgaa tcaggtccag aggtgccagt
     2641 ttctcttcac aaccttcaac aactacggtt tttccgcggc ggatttgctc agcggtcaga
     2701 ccttcagtat caatcgacag gatcttgcca gtgtgaccgg tgccgctaac cataacacgg
     2761 ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg tcataggtgt tttctccttt
     2821 ctgatttaca gtcgaagccc acgtaacggg cgcaatataa atactcctgt cgagtgaaat
     2881 attttttgtt tttgatcaag ctcacatctt ttttgatgca tgcatgagcc gtaaggcggg
     2941 gggaaaagcc tctcatcccg gatgggatga gagtaaatga attactcttc gctaaaccag
     3001 tcgctgtttt cctggcggat ctggcgcacg gattcgctga tttcctgcaa atgttgcgtc
     3061 attgcacgtt ctaccgcatc ggcatcacgt ttttgcaggg cagagaaaat atcaagatgc
     3121 tggcgtaaca gcatttctgg tggagaaaca tggtcgaaac tcatatagcg cacgcgatca
     3181 acggtcgctt tcaggttctc aatggtatcc cacgccagtt gacagtcggc aatctgcgtc
     3241 aggagttgat ggaagttgtc atcaagttca aaaaaatcat ccagttgctt gcgctcaatg
     3301 gcaatgcgtt gctggtgaag attttgttcc agttggtagc actggctttc ggtaatcatg
     3361 ctcgccgccc gacgcgccac cgcgcactca atggcctgac ggataaaact gccgttgcgc
     3421 acctgggcca tggaaatttt gttgacgtag ctgccacgct gcggacgaat ttgaatcagg
     3481 ccgttttccg ccagtttaat aaaggcttca cgaaccggct ggcgtgacac attgaaacga
     3541 acagaaactt ctttttccga caacggtgtg cctggtgcaa tcaggcaatg gacaatgtcg
     3601 cgacgaagaa tacgataaat ctgctgattg acaggctgtg tgggattaag ttgcgtttcg
     3661 acggtcattc gttcttgctt gtgagtgagt taactgcatg agtctaccac ttttttgtga
     3721 caagcaataa cccggcagtt acgccgggta taaaaattac tgacggtgga cattcagtcc
     3781 ggcatagctt tgggtaaccg gcatcatttc cagggtattg atattgacgt gagcaggcag
     3841 cgttgacacc caccagacgg cttcgctgac atcttctggc gtcaatgcaa cggtattttg
     3901 ataggttttt tccgctttac cgtcatcgcc tttaaagcgg acattggaaa actcggtgcc
     3961 acccaccaga cccggttcga tgtcggtgac gcgcaccgcc gtaccatgca gatccgtacg
     4021 cagattcagg ctaaactgac gaacaaacgc tttcgtcgca ccgtaaacgt taccaccggc
     4081 atacggccag ctgcctgccg ttgagccaat gttaataata tgaccatgat tacgttcaac
     4141 catacccggt aagacggcgc gcgtcatata tactaggcct ttgttgttgg tatcaatcat
     4201 cgtttcccag tcttcaacgc tggctttatg cgcaggctcc atgcccaacg ccaggccggc
     4261 attatttacc aggatatcaa tattgctcca ctcggcaggg agcgatgcca gcatctcttc
     4321 aatagcggcg cggttgcgaa cgtccagttg ggcgatatac agattatctc ccagttcgtc
     4381 tgttaactcc tgcaaccgct cctggcgacg gccagtggcg ataactttat gcccttgttg
     4441 aataaaacga cgagtaatgc attcaccaaa acctgccgtt gctccagtta ctaaaacgat
     4501 catctcactg ttcctcaacg cttttgtgtg tcaataacat agcacgtgat gcttaagagc
     4561 gggtaatact ggtttgcaag attgcactcc gttatcagta agcctacgct atggaacacc
     4621 tcgcgttcag gagaagaaaa tgacaacaat gaatcctttc cttgtgcaaa gcacactgcc
     4681 gtatctggct cctcattttg atcaaattgc caatcatcac tatcgcccgg cattcgatga
     4741 gggaatgcaa caaaagcggg cagaaattgc tgccatcgcg cttaacccgc aaacgcctga
     4801 tttcaacaat actattctgg cactggaaca aagcggagaa ttaattaccc gcgttaccag
     4861 cgtctttttt gcgatgactg cggcgcatac caatgatgaa ttacagcgtc ttgatgaaca
     4921 gttttccgct gaactggcgg aactggctaa tgatatctat ctgaacggtg aattattcgc
     4981 gcgggtagat gctgtctggc agcgccgtga atccctgggg cttgatagtg aatccatccg
     5041 cctggtggag gttattcatc aacgttttgt ccttgccgga gccaaacttg cacaagctga
     5101 taaagcaaaa ttaaaagtac tgaatacaga agctgcgacc ctgaccagcc agtttaacca
     5161 gcggttactt gcagcaaata aatccggcgg tctggttgtg aacgatatcg cgcagctggc
     5221 aggaatgagt gagcaagaga ttgcgctggc ggcagaggcg gctcgcgaga aaggtctgga
     5281 taacaaatgg ctgattccgc tgctgaatac cacccaacaa ccggcgcttg ccgaaatgcg
     5341 cgatcgtgcg acgcgtgaaa aactgtttat tgcgggctgg acgcgagcgg aaaaaaatga
     5401 tgccaatgat acccgcgcta tcattcaacg tctggtagag attcgcgcac agcaggcgaa
     5461 actgcttggt tttcctcatt atgccgcatg gaaaatcgcc gatcagatgg caaaaacgcc
     5521 agaagcagca ctcaacttta tgcgggaaat tgttccagcg gcgcgtcaac gtgcgagcga
     5581 tgaattagcc tccatacagg cggttatcga taagcagcag ggcgggttta gcgcgcagcc
     5641 gtgggactgg gcattttatg cggaacaggt acggcgggag aaatttgatc ttgatgaggc
     5701 gcagctcaag ccatattttg aattaaacac ggtgttaaat gaaggtgtat tctggaccgc
     5761 gaatcagctc ttcggtatta agtttgtcga acgttttgat attccggtct accatcctga
     5821 cgttcgtgtg tgggaaattt ttgatcataa tggcgtggga ctggcgttat tttacggtga
     5881 tttcttcgcc cgtgattcaa aaagcggcgg tgcatggatg ggcaattttg ttgagcaatc
     5941 aacgcttaat gaaacgcatc cggtaattta taacgtctgc aattatcaga aacccgctgc
     6001 cggtgagcct gcgttgttac tctgggatga tgtaataacc ttattccatg aatttggtca
     6061 tacgctgcac ggcctttttg cccgccagcg ttatgccacg ctttccggca ccaacacgcc
     6121 gcgtgatttt gtcgaatttc cgtcgcaaat caacgaacac tgggcaacgc atccgcaggt
     6181 attcgctcgc tacgcccggc attatcagag cggggcagca atgcctgacg aactgcaaca
     6241 gaaaatgcgt aatgccagcc tgttcaacaa agggtatgag atgagcgaac tgcttagcgc
     6301 cgcacttctc gatatgcgct ggcattgcct ggaagaaaac gaagcaatgc aggatgtcga
     6361 tgattttgaa ttgcgggcgc tggtggcgga aaatatggat cttcctgcta taccgccacg
     6421 ctatcgcagc agttatttcg cccatatttt tggtggcgga tatgctgcag gttattacgc
     6481 ttatctgtgg acgcaaatgt tggccgatga tggttaccag tggtttgttg agcagggcgg
     6541 attaacgcgt gaaaatgggc agcgttttcg cgaggcgatc ctttccagag gtaacagcga
     6601 agatctggaa cgcctgcatc gacaatggcg tggtaaggct cctcagatta tgccgatgct
     6661 gcaacatcgt ggcttgaaca tataaacacc accaatggct ggcctggaga tatcgccagg
     6721 ccagcctggt tatagcagta attgcagcag ctgggcgagg gcaaacctca ccgctaaagc
     6781 taataccgtt tcgcagtcgc cagcaaaatg cataaccgca gtgtagttct ggcctttaat
     6841 atgccacgcg aaccagacgg tacccgctgg cgtaccatct tcaccgccct ccggtccgcc
     6901 gtagccggta atggcaatac tgacatcagc atccgcacgc tctatggcac ctgttgccat
     6961 ttctgctgcc actttctcac tcaccgcaga atatcgttca agagactgct ggcttacgct
     7021 gaggattttc atctttgcct gatcggtgaa agtaacaaag cctgcaccgt aaaatttggg
     7081 tgtatcttca gctgcacaca gggcgctagc caacttaccg ccggtgcatg attcggcggt
     7141 agttaagcgt aattgatccg cgataagtct ttgactcaac gcggatgtca ggttttcaat
     7201 agtgtcggta tctgcgagtt gtacaatttt atctttatta atgttcactg ttttttgtcc
     7261 acgctattat atgtaaaggt gatttgcttt ttctgtatgc tcgtccaata taataatttt
     7321 caaacatata aatccgatta ttctgaaaac atgtttaaga ctcttcaatc ttcttaactt
     7381 tagtgagtca aattctctga atatttgaca gctagtgggc tgattttaaa tgccttataa
     7441 caattggcag tattcttatt tggcaattat aaaaatagca accagactta ataagtcaaa
     7501 ccaaacgcaa ttaaggagtt agtatgaaat ttcagtccat tgtattagca agttttcttg
     7561 tcatgcctta tgcgttggca gatgaccagg gcggtttaaa acaggatgca gcgccaccgc
     7621 cgcctcatgc aatagaagat ggctatcgcg gaaccgatga tgcaaaaaaa atgaccgttg
     7681 atttcgcaaa aaacatgcac gatggcgctt cggtttcact acgcggtaat ttgatttctc
     7741 acaaagg
//
LOCUS       DAOFMX010000145         7723 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.146, whole genome shotgun sequence.
ACCESSION   DAOFMX010000145 DAOFMX010000000
VERSION     DAOFMX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.146"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..835)
                     /locus_tag="QBA20_003953"
     CDS             complement(<1..835)
                     /locus_tag="QBA20_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HDQ6294301.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQ"
     gene            1017..2441
                     /gene="sbcB"
                     /locus_tag="QBA20_003954"
     CDS             1017..2441
                     /gene="sbcB"
                     /locus_tag="QBA20_003954"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDQ6294302.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2484..2711)
                     /locus_tag="QBA20_003955"
     CDS             complement(2484..2711)
                     /locus_tag="QBA20_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDQ6294303.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2725..3782)
                     /gene="yeeE"
                     /locus_tag="QBA20_003956"
                     /pseudo
     CDS             complement(2725..3782)
                     /gene="yeeE"
                     /locus_tag="QBA20_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
     gene            complement(3961..5319)
                     /gene="plaP"
                     /locus_tag="QBA20_003957"
     CDS             complement(3961..5319)
                     /gene="plaP"
                     /locus_tag="QBA20_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDQ6294304.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5309..5371)
                     /gene="yoeI"
                     /locus_tag="QBA20_003958"
     CDS             complement(5309..5371)
                     /gene="yoeI"
                     /locus_tag="QBA20_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDQ6294305.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5532..6515)
                     /locus_tag="QBA20_003959"
     CDS             complement(5532..6515)
                     /locus_tag="QBA20_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6294306.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6561..7385)
                     /locus_tag="QBA20_003960"
     CDS             complement(6561..7385)
                     /locus_tag="QBA20_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDQ6294307.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7468..7722)
                     /gene="yoeB"
                     /locus_tag="QBA20_003961"
     CDS             complement(7468..7722)
                     /gene="yoeB"
                     /locus_tag="QBA20_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HDQ6294308.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2066 a         2012 c         1812 g         1833 t
ORIGIN      
        1 tttgttcgaa gattggacgg cggataacat tacccatctt gctatgctct ttcggtacgg
       61 tccatacgtt gtccagcagg tcaaattctg tctttgttgc cagcctaagc tctgagagcc
      121 tcgcccccca cagcataagc atctgatgaa ggagcttatt tgacgtagac gcacggcttc
      181 tttcaatagc aagccaaatc ttagccagtt cgtgatacga cagtacccga tcccctacct
      241 cagcgcggga gccgaagtcc cttggctgga tgctcataat tgcgcaacta tctatcaact
      301 gacgccgcat gcaccaacta attgctgatc ttagttgact tagcacctgc cttgctcggc
      361 gtggattatc tttttcttct tcggtaagca ggtcaaccca ttgcttaacc gtgatagaag
      421 atgccggacg attagggaag gcgtcatgca tgcgcttcat aaccgttgat cgataaagtg
      481 cctgggtctt ttctctgaga gttgtagaga cgtagttgtc gaaccagtag tcgagacact
      541 gggcgaccgt catggagttc tccaccttct cttcaaaata ggtgcgtgga tccgttcctg
      601 agaaatagag ctttcgcaag tcagcagtga tctgtctggc atccttcaaa gacagggatg
      661 ggtatcgacc aagcccaagt cgattaggct tgccatgcca acgatagcgg tactggaact
      721 ggatgacccc cttcggtgaa attcgtacgc tgaggccatc ggcatcagcc acttcttgtg
      781 ggcccgaata tggtttacca taaatagtac gcagttttgt gtcgcttata gccataaata
      841 atatttggta cgctcagaaa taatagtttg gcacacatct ggtacacaat atcacatgga
      901 caacagcgca cagtaaacaa ctatatttga cgttcgtaga catactcagg tgaaaaaagg
      961 gttgcttttc gagttataac agacagttaa ttaactacag tggacaatgc tagacaatga
     1021 cagacacaga taaacaacct accttcctct ttcacgatta cgaaaccttt ggcacgcacc
     1081 ccgcgttaga tcgccctgca cagttcgcag ccattcgcac cgatgacgaa ttcaatgtca
     1141 tcggcgaacc cgaagtcttt tactgcaagc ccgcggatga ctatttaccc cggcctggag
     1201 cagtattaat taccggtatt accccgcagg aggcacgggc gaaaggagaa aacgaagccg
     1261 cgtttgccgc ccgtattcac tcgcttttta ccgtaccgaa gacctgtatt ctgggctaca
     1321 acaatgtgcg tttcgacgac gaagtcacac gcaacgtttt ttatcgtaat ttctacgatc
     1381 cttacgcctg gagctggcag catgataact cgcgctggga tttactggat gttatgcgtg
     1441 cctgttatgc cctgcgcccg gaaggaataa actggcctga aaatgatgac ggtctaccga
     1501 gctttcgcct tgagcattta accaaagcga atggtattga acatagcaac gcccacgatg
     1561 cgatggctga tgtgtacgcc actattgcga tggcgaaact ggtaaaaacg cgtcagccac
     1621 gcctgtttga ttatctcttt acccatcgta ataaacacaa actgatggcg ttgattgatg
     1681 ttccgcagat gaaacccctg gctcacgttt ccggaatgtt tggggcatgg cgtggcaata
     1741 ccagctgggt ggcaccgctg gcgtggcatc ctgaaaatcg caatgccgta attatggcgg
     1801 atttggcagg agacatttcg ccattactgg agctggatat cgacacattg cgcgagcgtt
     1861 tatataccgc aaaagccgat cttggcgata acgccgccgt tccggttaag ctggtgcata
     1921 tcaataaatg tccggtactg gcccaggcga atacgctacg cccggaagat gccgaccgac
     1981 tgggaattaa tcgtcagcat tgcctcgata acctaaaaat tctgcgtgaa aatccgcaag
     2041 tgcgtgaaaa agtggtagcg atattcgcgg aagccgaacc gtttacacct tcagataatg
     2101 tggatgcaca gctttataat ggctttttca gtgacgccga tcgtgcagca atgaaaattg
     2161 tgctggaaac cgagccgcgt aatttaccgg cactggatat cacttttgtt gataaacgaa
     2221 ttgaaaagtt gttgttcaat tatcgggcac gcaacttccc ggggacgctg gattatgccg
     2281 agcagcaacg ctggctggag caccgccacc aggtcttcac gccagagttt ttgcagggtt
     2341 atgctgatga attgcagatg ctggtacaac aatatgccga tgacaaagag aaagtggcgc
     2401 tgttaaaagc actttggcag tacgcggaag agatcgtcta atttgaagcc gccgccgctg
     2461 gtacggcgga ggacaatgtt gagtcaggct ttttgaacgg tgatgctcca cgctgcatcg
     2521 ccaatctgct gataatcggt gatggcatgt ccttcttctg ccgcccactg tgggattgct
     2581 tcagttgcct gggtgcaatc aaactcaatt accaactcat cgccgctggc catttctgcc
     2641 agtgctgctt ttgcttctat gagtggaaac ggacagacct gcgtcaccac atccagcttt
     2701 ttaatcgcca tctttacacc ttacttaatt tgccgcagca gttgccagtc gcgccttacg
     2761 ctgcggtcga acatacacaa gccaggacgc agtccagact ccgagaatca taaatacaag
     2821 gccaatccag ccctgccagg tcatcattgc agtcataacc agtccattac cgatagagca
     2881 accacctgca atactggcac cgaagcccat cagtacacca cctaacccgc tacgtaatgt
     2941 tgtttgtgca tcagctgcgc gaacgcggaa ctcacggctc gctttggctg caataaatgc
     3001 ctccacgaag atccctaaca ccaggaaaac gccccagtta atgtatttca cgtcacccgc
     3061 gaccagaaat tgaagaatat tggccgttgg ggaagtgact cctaacccaa acatgcgtcc
     3121 ggttgcttca ctcaggggcc aggctaaaag cgcaatcaaa ccgatgagta cagcagtgac
     3181 aaagggatgc cagcgttttt caaacagaat atgagcgatc ccggttcggc gcggcggtaa
     3241 ggtcgcgact ttgagttttg gcttcttcaa ctctttcatc accacccaga gcgttatcac
     3301 cagcaaaacg gtaaccaacg gccacacaga caaattaaaa gtctcagcaa tagagttatg
     3361 ttcagtagtg tagtgctgca aggtttgatt taaaccactg gcgtgtggag aacgcatcac
     3421 cgcactcatc accatataag tgaaaagcgc gatccaactg ccgatcaatc cctcacccgc
     3481 gcgataccag gtcccggtgg cacatccacc cgccagaaca attcccagcc cgaaaagata
     3541 cccacctata acagtaccaa gccacgggaa cgccccggct tcgtaagtca gtaggcccgc
     3601 ctgaattaac gcaaaacccc cacgctttgt gccgaaattg caatcagcaa ggcgtaaaac
     3661 atgcgattat ttttcgcgat atacatatcg cgaaaaccac cagtcaggca aaaacgccca
     3721 cgctgcatga caaatcccag cagagcacca caaattagcc cgcttaatat cattgaaaac
     3781 ataaattaat taaccagatg aatgttaatg gggaaatttt tcatgactgg tggaatgcag
     3841 accaataacc aaattctcta aattagaaca aatggttatt aatgaggatt tcgataaaaa
     3901 agaataaaaa aaccggagcc aaatgttcat tcgactccgg tttattatta gaaaagatga
     3961 ttatgctacg tcttcgtact gcggaaccgg attacggaag ctcttggtga cgcaagccag
     4021 gtaaatcaga ccgatagcag cccagatcag acccagaacc attgagcttt cttccaggtt
     4081 aacccacagc gcaccaacgg tcagcgcacc acacatcggc aggaacagat actggaagtg
     4141 atctttcagc gtcttgttac gcttctcacg gatccagaac tgcgagatga ccgacaggtt
     4201 aacaaaggtg aacgccacca gcgcaccaaa gttaatcagc gccgttgcca ttaccaggtc
     4261 gaagttgatt gccagcaggg caatcgcgcc aaccaggatg atgttcatcg ccggagtacg
     4321 ccatttcggg tgtacataac cgaagaagct tttcgggaac acgccgtcac gacccattac
     4381 gtacatcaga cgcgctacgc ctgcgtgcgc cgccataccg gacgccagta cggtaatggt
     4441 ggagaagatc agcgcgccaa cctggaacgc tttacccgca acgtacagca tgatttcagg
     4501 ctgtgacgca tccggatctt taaagcgaga gatatccggg aagtacagct gcaggaagta
     4561 agttgcaaag atgaagatca tgccgccaat cagcgcggtc aggaaaatcg cacgcgggat
     4621 cacgcgctct gcatctttgg tttcttccga cagattgctg atgccgtcaa agccggtaaa
     4681 ggagaagcac aggattgtcg ccccggtaat catcgggata acatgcgcat cgccagacca
     4741 gaacggacgc gtgctcgcca gcgtaccagc gccttcacct tcaaatacgc cataaacaac
     4801 catgcccaga atcaccgcga tcagcactac ctgcaacacg acgattacag tgttgacgtt
     4861 cgctacggat ttcagactac gcaggttaaa ggcggtcata aaggccacca gcgccaccac
     4921 aaacatccac gatggaatgg aaggcaccag agcttcaaaa tagattttcg ccagcagaat
     4981 gttgatcatc ggcgcgaaca gatagtcgag cagagaagac caacccacca taaagccgac
     5041 agtcgggcta atggatttct gggcgtaagt gtatgcagag ccagcagaag gatagcggcg
     5101 aaccagcttc ccgtagctca gagccgtaaa caggatcgca atcaatgcga acgcataggc
     5161 tgtcggcaca tgaccatccg tgaggcctga aacgatacca aatgtatcaa acagcgtcat
     5221 cggctgcata taggctagac ccatcattac aaccggaact aacgtaagcg ttttacgcaa
     5281 ttccacgcga gaggtgtttg gagtaacgtt atgcgacatg gttattctcc tttacggtga
     5341 taaccgtcgc gtaagcaaaa aattgcccca tttttttgga ttcctcagcg acaacaactg
     5401 tcgattttta gtaaatatct atccggtacg aagcccggcc tcttggtatg aatgattggt
     5461 ttgaagcaaa aaataaccga cgctaattaa acgtcggttt ttaatcattt tttgacagcg
     5521 gcgcattgtg cctaaatggg ggggaaatga caagagaatg agaggcttgt cataataatt
     5581 tttctttgaa tggctgattt tccgtcatca gattttgcat aaacaccggc gatcgcacta
     5641 tttgctaaaa tttcatctcg ccaccagccg ctggcaagcc ctgcggtctg ttcattccag
     5701 ccaatccaga caggttcaaa gagagtttgg gcgaccactt tcttctcgat taacgcgcca
     5761 ctatcgagaa aacgttgcgc cagataccgt ggtaaatagc cgcagccaag gccgctaatt
     5821 tgcagctcca gtttggtttt aaaatcgaaa acggtaatgg cttcttgttc gtcgagaagt
     5881 tgtgaggccg tagaagcgcc tgcctgcgca gtgtccccca ccacaatcgc ccgatatcgt
     5941 ttaatgatac gacggtttaa cggttcttct tcctgggcta acggatgatg aggcgcgacc
     6001 gcaaagactt gttcaagatc gcccaaccgc gagaaaccaa actcactgga agatggtggc
     6061 tcatgcatcg cgccaacgat aatatccgct cgcccctgag tcagggcatc ccaggaacca
     6121 ccgagtacgc cattgataaa cttcaggcgc gtcacgctat gatgttgata aaaagcttca
     6181 atgagaggcg caagcagaga aaaaggaaac gtgtcgtcca cgccaatcac cagctcgttt
     6241 tcccagcctt catgcagttt gatcgcctgc ttttccagtt ctcgcacggt atgcagaact
     6301 tcccgccctt tctctaataa catttttccg gtgcgggtga atttagcgcg gtgtccgcta
     6361 cgatcaagca attgaatatt taggtcgctt tccagcttgt gaacggtgta actcagagcg
     6421 gacggcgtct tgtagagttt ggctgacgcc gccgcaaaac tgccctcttt ttctatggca
     6481 tcgaggatca tcaacacatc cagcagtggt ttcatacacg cccccttgca gtgcatgcgg
     6541 cgtcctgctg gcgtgatcgt ttactccaac ggcattacca gcggatcggg atactggtat
     6601 tcaaatccca gttcattaca aatccgactg ccatcaataa tcttaccttt gccgctgtcc
     6661 agactatttc tgaactgcgg aggttgcagc cccagtaaac gggccatctg cggatagaaa
     6721 acattacgcg cagggtgagc tggcgcacat atattataga tgtgtccgcc tttaggtgcc
     6781 tgtaacaaca gagtgatagc gccaataaca tcttctaaat ggactaaatt aacaccatgt
     6841 tcaccatcag gggcggtttt tccggcaaag aagcgtcccg gatgacgtcc cggtccgacc
     6901 aggcccgcaa gacgcagaat atcgaccgaa gtaccgggta aattgtgcag ccagtcttcg
     6961 agttcttcta atactcgtcc actgttggtt accggattac gcggggtagt ttctttaacc
     7021 gtgccttgcg cgtcgccata gacagatgtt gagctggtaa aaataattcg gggaatacga
     7081 tgggccagcg cgctatccac taactcttgt accgcttgta aatagaactc atcgccgggg
     7141 ccgctacgac gtgccggaag cgtaatgacc agcgcatcgg catccataag ggcatccaga
     7201 tcgtcagaat cgcaaactaa ctcaggctcc atgcgaagca gatagctatc aatgccactc
     7261 attcgggccg cttcgacacc atcttgtgtg gttttactcc cggtgacttg ccagcctcgc
     7321 gctgaaagtg acatcgccag cggcatacct aaccacccta acccgacaat tgcgaccttt
     7381 ttcatccgtt atctcctgat acaccttaga tctataaggc tacgctagcg tatcaaaact
     7441 gacaattcat tctatgaatg aatctgttca ataatgataa cgacatgctg caatgagcag
     7501 tgaatcatca gtaaccgcgt ataccagacg gtgctcctct gtaatgcgtc gggaccagaa
     7561 acctgacaaa ttatgtttca ggggttctgg tttcccctta ccttcaaatg gtgttctgcg
     7621 ggtatctttg ataagttcat tgatcttttt aacaattcgc ttatctgttt cctgccagta
     7681 cagataatca tcccatgatt cctcagacca gattagtttc act
//
LOCUS       DAOFMX010000146         7540 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.147, whole genome shotgun sequence.
ACCESSION   DAOFMX010000146 DAOFMX010000000
VERSION     DAOFMX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.147"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..88
                     /locus_tag="QBA20_003962"
     CDS             <1..88
                     /locus_tag="QBA20_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDQ6294309.1"
                     /translation="VRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(236..715)
                     /gene="folK"
                     /locus_tag="QBA20_003963"
     CDS             complement(236..715)
                     /gene="folK"
                     /locus_tag="QBA20_003963"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDQ6294310.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(712..2130)
                     /gene="pcnB"
                     /locus_tag="QBA20_003964"
     CDS             complement(712..2130)
                     /gene="pcnB"
                     /locus_tag="QBA20_003964"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDQ6294311.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2169..3095)
                     /gene="gluQRS"
                     /locus_tag="QBA20_003965"
     CDS             complement(2169..3095)
                     /gene="gluQRS"
                     /locus_tag="QBA20_003965"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDQ6294312.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3132..3587)
                     /gene="dksA"
                     /locus_tag="QBA20_003966"
     CDS             complement(3132..3587)
                     /gene="dksA"
                     /locus_tag="QBA20_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDQ6294313.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3765..4469)
                     /gene="sfsA"
                     /locus_tag="QBA20_003967"
     CDS             complement(3765..4469)
                     /gene="sfsA"
                     /locus_tag="QBA20_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDQ6294314.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4484..5014)
                     /gene="thpR"
                     /locus_tag="QBA20_003968"
     CDS             complement(4484..5014)
                     /gene="thpR"
                     /locus_tag="QBA20_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDQ6294315.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            5088..7517
                     /gene="hrpB"
                     /locus_tag="QBA20_003969"
     CDS             5088..7517
                     /gene="hrpB"
                     /locus_tag="QBA20_003969"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDQ6294316.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1732 a         1935 c         2105 g         1768 t
ORIGIN      
        1 ggtacggaag tcactgtcgt tctcaaaatc ggtggagctg catgacaagg tcatcgggca
       61 ttatctgaac ataaaacact atcagtaagt tggagtcatt accggcgatg ggattgatat
      121 ttatctgcgg gaatttatat tatgtttctg caaaaatgtt taaactaaaa tttttattaa
      181 ggaaagagag gatgtatgat tttcagaaaa tatttatatt cgtaatataa ataaattacc
      241 atttgcttaa tttgtcaaat gctcttgtat gtaagacttc acgcaacgtc tccccatcag
      301 gaaacgccag ctccggtgca atctcaaaca gcggccacag cataaatcca cgattcttca
      361 tatcgtagtg cggaacggtc aggcgttcgg tatttatcac ttcattacca aacagcatga
      421 tatcgagatc cagcgtgcgt ggtccccagc gttcagcttt acgaacacga ccctgttgca
      481 gttctatgcg ctgtgtatgg ttgagtaact cttccggggc gggcgaagtt tcaagcgcca
      541 cggcggcgtt gaggtaatcg ggttgatctt gcggccccag cggtggggtg cggtaaaacg
      601 aagaaacggc aagaatgcgg ctttcaggga tatcgcctaa tgctttcagg gcagcattga
      661 cctgctccag cggagaggcc agattgctgc ctatggcaat atacgccact gtcatgtggt
      721 accctcacga cgcggtgcgc gtttgcgtgg acgacgagta cgacgacgcg gtgacggttc
      781 ttcatccagc tcgttaagca tccctttttg atctggtggt gcggaaacct ggaactcacc
      841 ccaccatttc accagacgct gcagttcagc attacgttca acttcagctc gcaaggccaa
      901 caggtcataa gccgcacgga acttaggatg ctccagcagt ttccatgcgc gtttaccctg
      961 acgacgggac atacgcaact gcaactgcca gatatcgcgg gttaacgtcg tcagacgttt
     1021 cgggattgcc agtgaacggc aggcttcgtc cagcacgtcg ttcatcgcca gcgcgaaagc
     1081 gtcgtgatag gtcaggccgc tttcctgggc gatcttctgt gccgtctcca gcagtgggta
     1141 ccagaacatg gcggcaaaca agaacgccgg gttcacgcgc atatcgttat ggatacgcgt
     1201 atcggtattc ttcagcacct gttcaatgat ccgctccatc gggctgtcgc cattttccgt
     1261 gaagtagcgg gtaatggtcg ggaacagcgg ctggaacaga tgatattcac acaacagctt
     1321 ataggtttcg taaccgtagc ccgcttgtag cagtttaagc gattcttcaa acaggcgtgc
     1381 cggtgggata tcgttcagca gggtagcgag gcgagggatc ggttctgcgg tttccgggct
     1441 gatgcgcata cccaatttgg cggcaaaacg taccgcgcgc agcatacgta ccggatcttc
     1501 acggtagcgc gtttccgggt taccaatcag acggataacg ccgtccttca gatccttcat
     1561 accaccaacg taatcacgga cggtaaaatc cgctacgctg taatacaggc tgttgatagt
     1621 gaaatcgcgg cgctgggcgt cttcttcgat ggagccgaaa atgttgtcgc gcagcaacat
     1681 gccgttttgc ccgcgttggg aggtcgtgcg gtcgctgacg ttaccttcgt ggtgtccacg
     1741 gaaggtcgca acttcgataa tctccgggcc aaacattaca tgagccagac ggaaacggcg
     1801 acccaccagg cggcagttac ggaacagttt gcgcacctgc tcaggcgtgg cgttagtggt
     1861 tacgtcaaaa tctttcggct ttttgccaag taacaggtcg cgcacgccgc cgccaaccag
     1921 ccaggcttcg tatcccgctt tgttgagcct gtacattacc ttcagggcat tttcactgat
     1981 atctttgcgg gaaatagcat gctgctcacg cgggatcacc gtcacctgtg gacgggcgac
     2041 tgcctgttca gcctcgcttt cctcgcggct tagcaccttg cggcaaaaat tagcgactcg
     2101 ggtaaaaata gtacacctcg gtagtgtcaa acatcattca ggacaaaaaa atagcggcta
     2161 atcatagctc agcatgacgc atttgagaat gttgaattta caattgccga ctcgggcacg
     2221 gcggtcagca tccagttttt gacggctgac tgaaggattt gctcgacgct gaaatcctgc
     2281 cagtgtgctt ctgcctgctg ccccagaaat tgaagtgccg cgattagtac cgggcgtgga
     2341 tcgcctttcg gcaacgcagg cgcatgattc tgcttggaaa gtttagcgcc ttgtggatta
     2401 agcgccagcg gcagatgaat gtaatctggc actttccagc caaaaagctg gtacagcggg
     2461 atttgcctta ctgttggttc aatcagatca gccccacgca ctatttctgt aacgccctgg
     2521 aaatgatcat caaccacaac agccaggttg taggcgaaca acccatcacg gcgatgaatg
     2581 ataaaatctt cccgtgccag tttttcgtcg gcgtgaataa tgcctcgcag caggtcagta
     2641 aattgcgtga ccggatgctg ctggcggata cgcactgcgg cgttgtctgg tccatgatgc
     2701 aacacccggc aatgaccgtc gtaaataccg ccaatgcttt gaatacgcgc acgcgtacag
     2761 gtgcagtaat aacttagtcc ttgttcatgt aaccaggcga gtgcttcacg ataggcgtcg
     2821 tgacgttgcg attgccagag aacatcgccg tcccagtgca gaccgtaatg ttccagctgg
     2881 cgcaggatag tttctgcggc accgggaact tcacgaggcg ggtcgatatc ttctatgcgt
     2941 accagccagc gaccttgccg ggcgcgagcc tgcaaatagc tgccgagcgc ggcgatcaga
     3001 gagccaaaat gaagctcgcc ggaaggagag ggggcgaagc ggccaatata ctgtgtgtct
     3061 gtcatctctt tgaacaaaaa ataaggcggg agcatttccc gcctgtggta aacgtgatgg
     3121 aacggctgta attagccagc catctgtttt tcgcgaattt cagccagcgt tttgcagtcg
     3181 atgcacagat cggctgtcgg gcgcgcttcc agacggcgaa taccaatttc aacaccgcag
     3241 gattcgcagt agccgaaatc ttcgtcttcc acttttttca gcgtcttctc gatctttttg
     3301 atcagcttac gctcgcgatc gcggttacgc agttcgaggc tgaactcttc ttcctgggct
     3361 gcacggtcta ccgggtccgg gaagttggct gcttcatcct gcatatgtgt aacggtgcga
     3421 tcgacttcat ccctgagttg attacgccat gcttccagaa tacgacggaa gtgcgccagc
     3481 tgggcttcat tcatatactc ttcgcccggc ttctcctgat atggttccac cccagcgatg
     3541 gcgagaatac tcagggacga tgttttacgg ttttgccctt cttgcatgtt gcttctcctt
     3601 aacacgcact atcgatcccc atgttcgggg gaaaaatgag gccgctataa atagcagatg
     3661 cttttccgga tagcaattat ctaaacgtaa cacttgacaa ctgtgtgagg aaaagcgtat
     3721 ttgcgcacgc gaccagaatg taaattaacc agttacttac tttactacaa tgtaaccggc
     3781 agtgattttt taagagccat gccttcagca gaaatttccg ctttgtaagc cagaatttct
     3841 acccccctct gttgagcttc tgacaatagt tgcgcgtatt tctcatcgat gtggcgcgcg
     3901 ggtgaaaacc gtgtaatggc tgaatgcagc acggcgaaaa aaataaccgc acgctggcct
     3961 tcagccgcta cgctcatcaa ctcccgaagg tgtttctgac ctcgttcagt gaccgcatcg
     4021 ggaaaatatc cctgttcgtt ctccgctaac gtaaccgatt tcacttcaat atagcagtct
     4081 ggacgcgaat ccgcctgcaa cataaagtca atacggctgc gttcggagcc gtattttact
     4141 tcgcttttca gcgagctata gcctgacagt tctgaaattg attcattaag gatagcctct
     4201 ttcgtcaacc tgttagccca aagcgtattg acgcaaataa atgcgccgct ctggctttga
     4261 gttaattccc aggtgtgtgg gtatttccgt ttggtgttgt ctgaagtcga ataccagacg
     4321 gtatcgccag gcgttgcaca accggtcatc gcacccgtat tcgggcagtg tagcgttaat
     4381 tcgcgaccat cgggtgtgat cacatcggct aaaaaacgtt tgtaacgctg aattagcgtc
     4441 gcgcgctgta gagggggaga aaattccatt gcgacaatcc ttgttattgc gttagcgccc
     4501 agcgttttag cggcgtgtag cgtgtgcgtc cacgggcaaa cgaggaggcg taaagggtga
     4561 actccgtcac cgcataagac cagttaaaac ccggcggcgg gaatgtcacc gcctcgctgg
     4621 cgtcgcgcaa taaggtaata tgtggatgaa acggacgatt gctttgaaaa caaccgctgc
     4681 gggcagcctg tgaacggagc atattcgcca gctggattaa gccgcgtgga ggctgacgca
     4741 tccctaacca caccacacgc gaacgcagcc attgtccggc gtcatcaagc gtgagtgtga
     4801 aaccaggttg acgaatccgt ccggctaaaa gagaaagcgc cttctctttc tctgcgctca
     4861 cttcgcctaa aaatgccaga gtcaggtgca aattatcggc ggcgaccgga cgtcccgcct
     4921 caggtgggaa gtgtgtggcg cgccaatgga taatctgttc gcggatttct gcaggtaagt
     4981 cgatagcaaa gaacagacgt tgcggttcag acatgtgagg cactcggtta taaattaccg
     5041 cgatgctaca atgtggcgcg aagaatgtta accctctgga gcgttttgtg tcgtcgttgc
     5101 ccgttgctgc cgtcttacct gaattactta ccgctctcga ttgtgcgccg caggtattat
     5161 taagtgcgcc gaccggggcc gggaaatcaa cctggctgcc gctgcaactg ctggcgcatc
     5221 ccggcattaa cgggaaaatt atcctgctgg agccgcgtcg tctggcggcg cgtaacgtcg
     5281 cgcaacggct ggcggagctg cttaacgaaa agccaggcga taccgttggc taccggatgc
     5341 gtgcgcaaaa ctgcgtcggg ctgaataccc gcctggaagt ggttaccgaa ggcgtgctga
     5401 cgcggatgat ccagcgtgac ccggaactga gcggtgttgg attggtgatc cttgatgagt
     5461 ttcatgagcg cagtttgcag gcggatttgg cgttggcgct gttactcgat gtgcaacaag
     5521 gtctgcgtga tgaccttaaa ctgctgatta tgtcggctac gctggacaat gaccgcttgc
     5581 agcaaatgct gccggaagcg cccgtcgtca tctcagaagg gcgctcgttt ccggttgaac
     5641 gccgttattt accgctgccc gcgcatcagc gttttgacga agccgttgcg gtagccaccg
     5701 ccgaaatggt gcgtcaggaa agtggatcat tactgttatt tttacctggc gtcggagaaa
     5761 ttcagcgcgt gcaggaacaa ctggcttcgc gcatcggcag tgatgtggtg ctctgcccgc
     5821 tgtatggcgt gttgtcactg aacgatcagc gaaaagcgat cctcccggca ccgcaaggga
     5881 tgcgcaaagt ggtgctggcg accaatattg ctgaaaccag tttaaccatc gaaggtattc
     5941 gtctggtggt ggattgtgcc caggagcgtg tggcgcgctt tgatccgcgc acggggctta
     6001 cgcggttgat tactcaacgc gttagtcagg catccatgac gcagcgtgcc gggcgcgccg
     6061 ggcgtctgga gccgggtatc agcctgcatt taatcgccaa agaacaagca gaacgcgccg
     6121 cggcgcaaag tgagccggag atcttacaaa gcgatctttc cggtttgctg atggaattac
     6181 tgcaatgggg atgcagcgat ccggcgcaga tgagctggct ggatcaaccg ccaacggtga
     6241 atctactggc cgcgaaacgc ctgttacaaa tgctgggggc gctggagggt gaacggctta
     6301 gtgcgcaagg gcaaaaaatg gcagcgctgg gtaacgatcc gcgtttagcg gcaatgctgg
     6361 ttaacgcaaa gagcgatgac gaagctgcta ccgcggcaaa aattgccgcc attctcgaag
     6421 agccgccacg gatgggcaat agtgacctgg gcgtggcgtt ttcgcgcaat caaccagcct
     6481 ggcagcaacg tagtcagcaa ctgttaaaac gcttaaacgt acgcggcggt gaggcagaca
     6541 gttcgcttat cgcgccgcta cttgccgggg cgtttgccga tcgcattgct catcgccgtg
     6601 ggcaagatgg acgctatcaa ctggcaaaca gcatgggagc gatgctcgat gccgacgacg
     6661 cgctaagccg ccacgaatgg ttgatcgcac cgttattatt gcagggcagc gcctcgccgg
     6721 atgcgcggat tttactggcg ctaccggtcg atattgatga attagtacaa cgctgtccgc
     6781 agctggtgca gcaatctgac accgtggaat gggatgacgc gcaaggtacg ctgaaagcct
     6841 ggcgtcggct acaaatcggt cagttgacgg tgaaagtgca gccgctggcg aaaccgtcag
     6901 aagacgagtt gcatcaggcg atgcttaatg gcatccgtga taaaggttta agcgtgctca
     6961 actggacggc ggaagcggaa cagctacgct tgcgtttgtt atgcgccgca aagtggttgc
     7021 cggaatatga ctggccagcg gttgatgatg aaagtttgtt ggcgacgctg gaaacgtggc
     7081 tgctgccaca tatgaccggc gtacattcac tacgcggcct gaaatcactc gacatttatc
     7141 aggcactacg cggattactt gattggggaa tgcagcaacg tctggatagt gaattgcctg
     7201 cgcattacac tgtgccgacg ggaagccgga tcgccattcg ttatcatgaa gataacccgc
     7261 ccgcgctggc ggtgagaatg caggagatgt ttggtgaggc cactaatccg acgatcgccc
     7321 aggggcgcgt gccgctggtg ctgaagttgc tttcccctgc ccaaaggccg ctgcaaatca
     7381 cgcgtgattt gggggccttc tggaaagggg cgtatcgtga ggtgcaaaag gagatgaaag
     7441 ggcgttatcc caaacacgtc tggccggacg acccggcaaa cacggcaccg acgcgacgga
     7501 cgaaaaagta ttcgtaaggt tggttttctc cctctccctg
//
LOCUS       DAOFMX010000147         7413 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.148, whole genome shotgun sequence.
ACCESSION   DAOFMX010000147 DAOFMX010000000
VERSION     DAOFMX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.148"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="QBA20_003970"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="QBA20_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="QBA20_003971"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="QBA20_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDQ6294317.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="QBA20_003972"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="QBA20_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="QBA20_003973"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="QBA20_003973"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDQ6294318.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANISFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="QBA20_003974"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="QBA20_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDQ6294319.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="QBA20_003975"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="QBA20_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDQ6294320.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="QBA20_003976"
     CDS             complement(6510..7202)
                     /locus_tag="QBA20_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDQ6294321.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1882 a         1794 c         1948 g         1789 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatggacgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctagatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ctggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tctcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtat tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaa
//
LOCUS       DAOFMX010000148         7354 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.149, whole genome shotgun sequence.
ACCESSION   DAOFMX010000148 DAOFMX010000000
VERSION     DAOFMX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.149"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(617..1102)
                     /gene="sixA"
                     /locus_tag="QBA20_003977"
     CDS             complement(617..1102)
                     /gene="sixA"
                     /locus_tag="QBA20_003977"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDQ6294322.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1305..3449)
                     /gene="fadJ"
                     /locus_tag="QBA20_003978"
     CDS             complement(1305..3449)
                     /gene="fadJ"
                     /locus_tag="QBA20_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDQ6294323.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3449..4759)
                     /gene="fadI"
                     /locus_tag="QBA20_003979"
     CDS             complement(3449..4759)
                     /gene="fadI"
                     /locus_tag="QBA20_003979"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDQ6294324.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4939..5223)
                     /locus_tag="QBA20_003980"
     CDS             complement(4939..5223)
                     /locus_tag="QBA20_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDQ6294325.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5595..6935
                     /gene="fadL"
                     /locus_tag="QBA20_003981"
     CDS             5595..6935
                     /gene="fadL"
                     /locus_tag="QBA20_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDQ6294326.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1909 a         1914 c         1691 g         1840 t
ORIGIN      
        1 aatgtttata cccagccatt gaatacggca tgcgacccaa aaagaacagg tcgtagaaaa
       61 ttgtattcat tggtgtgggg ccgcatagtg atgtttccac acccctgatc caatttaatt
      121 ttactaaaga gtaaatgtag atttaattaa tatattgaca gaagaaaggt tatcttaagg
      181 acatccttaa aaaatacgga ataagtattt agaaaaaata gaaagtatat caataatgaa
      241 tatcaacagc ttaaaaaaca aacaatgaac atataatgcg attggcattt atccactttt
      301 gacaatataa tatataaaca ctatagccag aatttaatca agtttcaatt acatcctgcc
      361 tgattattca actctatttt ctctattatc tgtattaata aatgacaatg agtgtcaaac
      421 agatattatt catttcaaca tgtaactttg ggcaaaatca agatacgcgc agaagaataa
      481 tttccgaatg aatgtagaaa gtgttttatt tatagataag gagagtcttc gatagtagtg
      541 aggttgacag atatccgctg cctaatgagc aattatgccc aatgaatcta cctcattggg
      601 cataatttgg aacacatcag atagcttttg ccatcttcag attacacggg ctcatctgcc
      661 agttgaatgt accgtttccg ctttcatcaa gcgttacgct ggcaatggcc gaggtggtaa
      721 acataggcgg cgtttcgccc ggacataact cggcaaccag atacccgact aacggtaagt
      781 gagagatcac cagtaccgaa gcaacacctt cattggtcaa cgcctgcaaa taggcactga
      841 ccagaccaac atcgccgcag ggcgttaact ccggcagaac ttctgcactg gaaggcaggt
      901 tcagacaatc ccctacttct tccagtgttt gctcggctcg caggaacggg ctcaccagaa
      961 cgcgttcgat ttccactttt tgacctttca gccagttcgc catcaggcga gattcgtcac
     1021 aaccattagt ggtcagagga cgaaccgaat cactggcgga atcgagggct gcgtcgccgt
     1081 gacgcatgat aaaaacttgc atattgcacc gcttttgtta accagattcg cccgttttct
     1141 ttcatctgaa accagagaaa gaaaacggtg gccggcattg tgccttatcc attcaccgaa
     1201 tgaaacgctg ttttttacct caatggcgta agtatagtca atatgcgttt acattttgac
     1261 caacactccg ccattcagcg cggattcata tagctttgac cttcttattg caggtcagtt
     1321 gcagttgttt tccaaaaact ttccccacgc gcgcccatct cgaccaaacg ctcgcaaggg
     1381 gtaaaacggg aaccatactg cgtggccagt cgttgcatta ttgcaaccac ttcgcccgcg
     1441 ccgagagaat cgatatagcg gaacggtcca ccgagaaatg gcggaaaacc aatgccaaat
     1501 accgcaccaa tatccccgtc acgcacgcta cggataacct gctcatcaac acaacgtact
     1561 gcttcattca gcatcaacat cacacaccgt tcagcaacct gcggtgcgga gagtcgcccc
     1621 tgcccttgtg cgccaatgag cgggtaaatg gcaggatcga cctgtttttt gcttttacgc
     1681 cctttctgac cataaagata gaaaccccga ccattttttc tgcctttgcg atcgtcgttc
     1741 aaaattgaag aaacaacatt tgcaggcgcg ctaaaacgtt ctccataagc ggcttccagt
     1801 acaggaataa ttttagtccc ggtgtcgatt cctacctcat ccaaaagttg gattgggcct
     1861 accggaaaac caaatttcac tagcgcggca tcaatgtgct ctacccgttc accttcggtc
     1921 agcatgcgga tagcttcatt aatgtaaggc gctaagatgc gattgacgta aaacccggct
     1981 ttgtcacgca cgacaatggg cgttttgccc tgttttttcg ccagttttac cgtagtggcg
     2041 atggtttgcg ccgatgtacc cgcatgagga ataatctcta ccagcggcat tttttccacc
     2101 ggactgaaga aatgcaggcc aataacttgc tcaggtcgcg cggcgtgagc ggcgatatca
     2161 ccaatcggta aagatgacgt gttcgaagca aaaatggtat gagcggcgca attttgctcc
     2221 acttccgcca ccatctgttg ttttaattcg agattttcaa acaccgcttc aataatcaga
     2281 tcgcgatggg caaagccgcg atagtccgtc gttccggaga ttaatgccag ctgtttgtca
     2341 cgttcgctgg ctttgagatg acgacggcga actttgccct ccagctgatc ccaactgtac
     2401 ttcagcgcat gatttatgcc ctgcgggttg atatctttaa ttctgaccgg aagccccgct
     2461 ttacaagcag tgacataagc aataccgccg cccatcaagc caccacctaa aattcccacg
     2521 ctgtttaatg gcgcaggcgg tgcatcacta ccgggatctt tcttcacgtc cgtactggca
     2581 aaaaagatac tacgcaaagc ttgcgattgc ggcgtcatcg ccagttcgcc aaaggcgcga
     2641 gcttcagcgt catagccgct gctggtgccc tgcgctaatc ccgtttcaac aacctccagg
     2701 atgcgttctg tcgccggata attgccttga gttttgtgtt ctgttttctt gccgaccatt
     2761 ttgaacagca gcgcacgacc taacggcccc gccagaatac gctcgcgtac aggtagaggg
     2821 cgggaagatg ggcgatcctt cttcgccagc tcaacagcgg cttccagcaa aatggagtgc
     2881 ggaacaacgt catccaccag ccccagcttt aatgcctgtt tcgcccgaag ttgttttccg
     2941 gtgagaatca tctctaatgc tgtactgacg cctatcagac gcggtaaacg ctgggtgccg
     3001 cctgaaccgg gtaacaatcc aagttgtact tcaggcaaac cgagcaccgt tttaggatcg
     3061 tcagtacaaa cgcgaccgtg gcacgccagc gccagctcca gcccaccgcc caggcaagcg
     3121 ccatgaatag ccgcgataac cggaatgggc aaagcatgaa tctccgccat caactgttgg
     3181 ccctgccgcg ccagtgcttc cgcttcttgc gccgttttgc agttgccaat catgttgatg
     3241 tctgctccgg cgataaaatt gtccggtttc gcggagataa acaccacgcc gcgcaactct
     3301 ttgttttcac ggagttgctt aataatggcg cgcacctgcg aggcaaattc cgccttcagg
     3361 gtattcattt tctcatccgg tacgtcgatg gtgatgatag caatgttgtc cagacgaaca
     3421 ttaagagtaa acgctgatgc catttccatt attccgcctc cagaaccatt gccgcgccaa
     3481 gcccaccggc agcacaggcg gtaactaaac caaatccacc gccgcgacgg cgaagttcat
     3541 gcaatgtctg ggtaatcatc cgcgcgccgg tcgccgcgaa gggatgtccg taagcaatcg
     3601 aaccgccaag cacgttaaat ttgctatcgt ccacttcgcc agtggcatgt gcacgcccca
     3661 gtacttcacg agcaaaacgt tcactaccca gcaactgaat attcgccagt gtctgagctg
     3721 caaaggcttc gtgcatatcg atcaatgtca gttcgctcat cgtcaaaccg gcacgttcca
     3781 gcgccagcgg tgttgaccag gctggaccga gcaacatgtc ctgccagaca tcaatcgcag
     3841 taaatgcgta gctgcgcaga taccccagcg gcaccagccc taattctttc gcccgggatt
     3901 cagtcatcag gatcaccgct gccgcgccat cggtcagcgg cgtactgttt gccgccgtta
     3961 ccgttccgtg tttgcgatca aacgccgggc gcagctttgc gtaatcggca agcgaggaat
     4021 taccgcgaat attgttgtct tcgacaagcg gttgtttata aggagggata aaggcagtca
     4081 tcacctcttc tttgagtttt ccgtctgacc atgcctgagc ggcacgctga tgcgaacggt
     4141 gcgctaatgc atcttgctgt tctcgggtga tgccgtaggt tttcgccatt tgttcggcgg
     4201 tgtcgcccat ccgcaagcca gtagaatatt ctgctaccgc aggcggtacg ggcattaagt
     4261 cgcgcaaacg caggcgagag aagagtttca ggcgctggct catggttcga gctttgttga
     4321 catcaaccag cacgtgcgcc agttttttac tgacgccaat tggcaatacg gaagaggaat
     4381 ctgccccacc ggcaatcccc gctcggatag ttcccgccat caggctttct gcgacgtttg
     4441 caaccgcctg gaaactggta gcgcaagcgc ggctgacgct gtaagcatcg gtatgcacat
     4501 tcattccggt accgagaaca atttcacgcg caatgttggg ggcttcaggc atttgtacga
     4561 cctgaccaaa gaccagctgt tcaatcactt cagcggggat ctcgctgcgt gccagcagtt
     4621 cgcctaccac catcttccct aaatcaaccg tgggaatgcc atgaaaagcc gtcgcctggc
     4681 gggcaaaagg cgtacgtaaa ccgctaacaa tggcgatacg atcgccctgg cgggtaacca
     4741 gtggtaaaac ctgacccata acactcccct gtaaaaaaat aaataaagtg gtctgacctg
     4801 atcatagtct taaccatttt tttacattta gccaagcgga gaaaaaggaa agtgggagct
     4861 atgacacagt gaagacagaa gaaagaaaag aaaacgcccc tgccagcaac tgacaggggc
     4921 gtacgtaccc gagaggaatt aacgcagacc cagctggaag atcagcattt cagcttcgca
     4981 ggcaaaagtg aaatcgatat ccaggcgcac accgtcagac tcttcagtga aagtcgggga
     5041 gattttgcac ggttcggatt ccacgctacg ggatttttca gtcagcgccg ccagcgtttg
     5101 ttctgcttct gcgcggtttg caaacacgcg gctgtaagac gcggtgcagt cggagttgtc
     5161 cataatggtg ccaacatcca tacagcagca aaccggggtt tcatcagcac tacatttact
     5221 catcgttgat ttcctctgta tgtgcaccca aggtgccaga taaacgttgt ggatatttta
     5281 cgcttccgga aagtgctgct ccagttgtta attctgcaaa atcggataag tgaccgaaat
     5341 cacacttaaa aatgatctaa aacaaaattc acccgaatcc gcgagtgcgc aacctccaaa
     5401 ttttgccagc tggatcgcgt ttcttagatc atatttgaaa aaagatggaa acatacttgc
     5461 aacattctag ctggtccgac ctatactctc gccactggtc tgatttctaa gatgtacctc
     5521 agaccctaca cttcgcgctc ctgttacagc acgtaacata gtttgtataa aaataaatca
     5581 ttgaggttat ggtcatgagc cagaaaaccc tgtttacaaa gtctgctctc gcagtcgcag
     5641 tggcacttat ctccacccag gcctggtcgg caggctttca gttaaacgaa ttttcttcct
     5701 ctggcctggg ccgggcttat tcaggggaag gcgcaattgc cgatgatgca ggtaacgtta
     5761 gccgtaaccc cgcattgatt accatgtttg accgcccgac attttctgcg ggtgcggttt
     5821 atattgaccc ggatgtgaat atcagcggaa cgtctccgtc tggtcgtagc ctgaaagccg
     5881 ataacatcgc gcctacggca tgggttccga acatgcactt tgttgcaccg attaacgacc
     5941 aatttggttg gggcgcttct attacctcta actatggtct ggcaacagag tttaatgata
     6001 cttatgcagg cggctctgtc gggggtacaa ctgaccttga aaccatgaac ctgaacttaa
     6061 gcggtgcgta tcgcttaaat aatgcatgga gctttggtct tggtttcaac gccatctacg
     6121 ctcgcgcgaa aattgaacgt ttcgcaggcg atctggggca gttggttgct ggtcagatta
     6181 tgcaatctcc tgccgggcaa actcctcaag ggcaagcatt ggcagctacc gccaacggta
     6241 tcgacagtaa taccaaaatc gctcatctga acggtaacca gtggggcttt ggctggaacg
     6301 ccggaatcct gtatgaactg gataaaaata accgctatgc actgacctac cgttctgaag
     6361 tgaaaattga cttcaaaggt aactacagca gcgatcttaa tcgtgcgttt aataactacg
     6421 gtttgccaat tcctaccgcg acaggtggcg caacgcaatc gggttatctg acgctgaacc
     6481 tgcctgaaat gtgggaagtg tcaggttata accgtgttga tccacagtgg gcgattcact
     6541 atagcctggc ttacaccagc tggagtcagt tccagcagct gaaagcgacc tcaaccagtg
     6601 gcgacacgct gttccagaaa catgaaggct ttaaagatgc ttaccgcatc gcgttgggta
     6661 ccacttatta ctacgatgat aactggacct tccgtaccgg tatcgccttt gatgacagcc
     6721 cagttccggc tcagaatcgt tctatctcca ttccggacca ggaccgtttc tggctgagtg
     6781 caggtacgac ttacgcattt aataaagatg cttcagtcga cgttggtgtt tcttatatgc
     6841 acggtcagag cgtgaaaatt aacgaaggcc cataccagtt cgagtctgaa ggtaaagcct
     6901 ggctgttcgg tactaacttt aactacgcgt tctgataacg cgttcgcctg gataaagtca
     6961 cctgctatgc aggtgacttt aactccccca cttcaccgaa agtagtttcc tcattgcttg
     7021 cctcctgagt tttgcaaaca gcctgatggc agcttgccct ttcaaatcag taagtagtat
     7081 ccacagacaa acctgaaggg aaatgcatat tttcaagcgt tctgctcgtc cttctcaaag
     7141 agtttacttt tctgcatttc caggatactc aacccccctg gctattgtgc gcttatacac
     7201 tcaaattaaa gataggttct aaataaatga gcgttttttg atagtctatt tcattaggta
     7261 atatatattt gtaacaaatc aatcaacatg gaataaaatc atgctaccat ctatttcaat
     7321 caacaatacc agcgcagctt acccagaatc catc
//
LOCUS       DAOFMX010000149         7200 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.150, whole genome shotgun sequence.
ACCESSION   DAOFMX010000149 DAOFMX010000000
VERSION     DAOFMX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.150"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(275..362)
                     /locus_tag="QBA20_003982"
     tRNA            complement(275..362)
                     /locus_tag="QBA20_003982"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(326..328),aa:Ser,seq:gga)
     gene            597..1535
                     /gene="ghrA"
                     /locus_tag="QBA20_003983"
                     /pseudo
     CDS             597..1535
                     /gene="ghrA"
                     /locus_tag="QBA20_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            1590..2327
                     /gene="ycdX"
                     /locus_tag="QBA20_003984"
     CDS             1590..2327
                     /gene="ycdX"
                     /locus_tag="QBA20_003984"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDQ6294327.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            2351..2905
                     /gene="ycdY"
                     /locus_tag="QBA20_003985"
     CDS             2351..2905
                     /gene="ycdY"
                     /locus_tag="QBA20_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDQ6294328.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3007..3498
                     /locus_tag="QBA20_003986"
     CDS             3007..3498
                     /locus_tag="QBA20_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDQ6294329.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3631..4337)
                     /locus_tag="QBA20_003987"
                     /pseudo
     CDS             complement(3631..4337)
                     /locus_tag="QBA20_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(4364..4780)
                     /gene="csgF"
                     /locus_tag="QBA20_003988"
     CDS             complement(4364..4780)
                     /gene="csgF"
                     /locus_tag="QBA20_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDQ6294330.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(4805..5194)
                     /gene="csgE"
                     /locus_tag="QBA20_003989"
     CDS             complement(4805..5194)
                     /gene="csgE"
                     /locus_tag="QBA20_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDQ6294331.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            complement(5199..5849)
                     /gene="csgD"
                     /locus_tag="QBA20_003990"
     CDS             complement(5199..5849)
                     /gene="csgD"
                     /locus_tag="QBA20_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDQ6294332.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            6601..7056
                     /gene="csgB"
                     /locus_tag="QBA20_003991"
     CDS             6601..7056
                     /gene="csgB"
                     /locus_tag="QBA20_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDQ6294333.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            7097..>7200
                     /locus_tag="QBA20_003992"
     CDS             7097..>7200
                     /locus_tag="QBA20_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HDQ6294334.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
BASE COUNT         1912 a         1567 c         1728 g         1993 t
ORIGIN      
        1 tttccattgt cgattcctct ggcataacga ttttatgatg gcatgtgaca tgtatttccg
       61 ttgggggcat tttaataagt gaggaagtta caggaagtga ccagataacg catatatgtt
      121 ctgtactatc ttacgcattt tgattgttga ctgagtaacc agacagttga tgtgcacaat
      181 ttcccctcgc cctaacagac gtgggcgggg gcacccggtg tgtacaatat acgaaaaaaa
      241 agcccgtact ttcgtacgcg ctcttcttta aatatggcgg tgaggggggg attcgaaccc
      301 ccgatacgtt gccgtataca cactttccag gcgtgctcct tcagccactc ggacacctca
      361 ccaaattgtc gttcctgtct tgctggaacg ggcgctaatt tagggaaatc atgacctgag
      421 gtcaacaaac tttttgaaaa aatcgcgcgt ttattcaaac attcaatcaa tgtgtggttt
      481 taataagcga aatctgcttt ttttgccacc gaccacggat ttgttatgct ggtggccttt
      541 gtagatcata acgataagtg cgaataaatt tcgcacaacg cttttcggga gtcagtatgg
      601 atatcatctt ttatcaccca acgttcgata cccaatggtg gattgaggca ctgcgcaaag
      661 ctattcctca ggcaagagtc agggcatgga aaagcggaga taatgactct gctgattatg
      721 ttttagtctg gcatcctcct gttgaaatgc tggcagggcg cgatcttaaa gcggtgttcg
      781 cactcggggc cggtgttgat tctattttga gcaagctaca ggcacaccct gaaatgctga
      841 acccttctgt tccacttttt cgcctggaag ataccggtat gggcgagcaa atgcaggaat
      901 atgctgtcag tcaggtgctg cattggtttc gacgttttga cgattatcgc atccagcaaa
      961 atagttcgca ttggcaaccg ctgcctgaat atcatcggga agattttacc atcggcattt
     1021 tgggcgcagg cgtactgggc tgtaaagttg ctcagagtct gcaaacctag cgctttccgc
     1081 tgcgttgctg gagtcgaacc cgtaaatcgt ggcctggcgt gcaaagcttt gccggacggg
     1141 aagaactgtc tgcatttctg agccaatgtc gggtattgat taatttgtta ccgaataccc
     1201 ctgaaaccgt cggcattatt aatcaacaat tactcgaaaa attacctgat ggcgcgtatc
     1261 tcctcaacct ggcgcgtggt gttcatgttg tggaagatga cctgctcgcg gcgctggata
     1321 gcgggaaagt taaaggcgca atgctggatg tttttaatcg tgaaccctta ccgcctgaaa
     1381 gtccgctctg gcaacatcca cgcgtgacga taacaccaca tgtcgccgcg attacccgtc
     1441 ccgctgaagc tgtggagtac atttctcgca ctattgccca gctcgaaaaa ggggagaggg
     1501 tctgcgggca agtcgaccgc gcacgtggct actaataaag catcaggatt cctgctatcc
     1561 ttggcgggaa ttgaatacag gagagagtta tgtatcccgt cgaccttcat atgcataccg
     1621 ttgccagcac acatgcatat agcacattga gtgattacat tgcccaggcc aaacaaaagg
     1681 gcattaaact ttttgcgatc accgatcatg gcccggatat ggaagatgcg ccgcatcact
     1741 ggcacttcat taacatgcgt atctggccgc gagtggttga tggggtaggg atcctgcgcg
     1801 gcattgaagc taacattaaa aatgttgatg gtgaaattga ctgcagcggt aaaatgtttg
     1861 actcgctgga tctgattatt gccggttttc atgagccggt ttttgcgcca catgacaaag
     1921 cgaccaatac acaagcgatg atctccacta tcgccagcgg caatgtgcat ataataagcc
     1981 atcccggaaa tccgaaatat gaaatagatg ttaaagccgt tgctgaagca gccgcgaaac
     2041 atcaggtggc gctggaaatc aataattcct catttttaca ctcacgtaag ggcagtgaag
     2101 acaactgtcg tgcggtagct gcagcggtac gtgatgctgg aggttgggtg gcattaggct
     2161 cggattctca caccgcgttt accatggggg aatttgaaga gtgtcttaaa atcctcgacg
     2221 cggtagattt tccgctagag cgcattttga atgtttctcc gcgccgctta ctgaacttcc
     2281 ttgaatctcg cggtatggca ccgattgcgg aatttgcaga cctttaatta ctagtaatgg
     2341 aaatatatta atgaacgagt tttctatcct ctgtcgtgtg ctgggttcgc tctattaccg
     2401 ccaaccgcaa gatcctttac tggtgccgct gtttactctg attcgtgagg ggaaactggc
     2461 tgcgaactgg ccactggagc aggatgagtt actgacgcgt ttacagaaaa gctgtgatat
     2521 gacgcaagtc tctgccgatt acaatgcgtt gtttatcggc gatgaatgtg ccgtgccgcc
     2581 atatcgtagc gcatgggttg aggacgcaac ggaagcggaa gtgcgcgctt ttctttccga
     2641 acgagggatg ccactggcgg atacgccagc cgatcacatc ggcacattgc tgcttgcggc
     2701 ttcctggctg gaagatcagt caacggaaga tgagagcgaa gcactggaaa cactgttcag
     2761 tgagtatctg ttaccctggt gtggtgcgtt cctcggcaaa gtggaggccc atgcaaccac
     2821 gcctttctgg cgcaccatgg caccgctaac ccgtgatgcc attagtgcaa tgtgggacga
     2881 gctggaagaa gattctgaag agtaaatgtg atcatcgtca ctcataactg caacagataa
     2941 tttgttgttg cataaaatgt gtgctcgatc tcattcatgg ccgcgttttc tgctatcatg
     3001 cgcggcatga acatacttct ctctattgca atcacaacag gcattctctc cggtatctgg
     3061 ggatgggtgg ctgtttccct gggcttacta agctgggcgg gcttcctagg ctgtacggcc
     3121 tactttgctt gcccgcaagg tgggctgaaa gggctggcga tctccgctgc aaccctgctt
     3181 agtggcgtgg tgtgggcgat ggtcattatt tacggtagtg cactggcacc acatctggaa
     3241 attctcggtt atgtcataac cggtattgtc gcgtttctga tgtgtattca ggccaaacag
     3301 ctgctgcttt catttgttcc ggggacgttt ataggcgcat gcgcgacgtt tgccgggcag
     3361 ggtgactgga aactggtgtt accttcgctg gcgcttgggc tggtatttgg ttacgcaatg
     3421 aaaaacagcg gcctgtggct ggcggcgcgt agtgcaaaga cggctcaccg tgagcaggaa
     3481 atcaaaaata aagcgtgagg ggcactcacg ctttcgctta aacagtaaat gccggattat
     3541 taattccggc atttttatct gtcaggattc cggtggaacc gacatatggc ggtatttcac
     3601 cagaatgtca ttctgccgtt ctgctttatt ttacaaatcc cacagaccac ggtcgatacc
     3661 atcattaatc aggaaaatga cccctgtttc gatagccgac atcagacaca gcataacggg
     3721 ttcgtttgag gtgtaaccca cttccccttc cagcaagcgc tggtagtcaa taaagcggaa
     3781 aaccccggcc tgaacttcat aggaaagtat cgtcttactg gtgttcaccg aggagaggat
     3841 ctcaccggta ctcacattga cgacacgcag gttcacggca atctgatcga gctggtattg
     3901 cgtgtctgca ccgatcccaa aatatcttgc cccaacaccg cccgatttaa cgttgctttc
     3961 ataaccgata atcgaacctt caaccataat atttgccgcc gttaaagatt gcagcgggat
     4021 tcggttatta atggcaaccg tgccgttttc ttgtgccgca cgaataacct tgcgctcgtt
     4081 aagcaggttt tgtaagccct ggcgctttgc ggaacagcag tggagaagtt acttgccggg
     4141 tagggtttaa attgcccggt ttcgtcctga atgttgtata ccgaaacaaa gatcttaccc
     4201 gtcggcgctg gcagatgggt caaatctttg tagctctgag cacgaggcat taatgtcggt
     4261 ctggcggctt ctttaggcgg ggcggttaag catccgctca gtaacatgac ggcaaccaaa
     4321 agaaataagc gctgcatgat tattttcctt atgaagctgg ggcttaaaaa tcggttgagt
     4381 tattttgtaa acccgaaacc tggatggtcg aggtttgtcc ggttttacga tctgtcacgt
     4441 tcaactgcaa ttgaccatcg cggttagcaa tatcaacaat ataatcgttg gtcaccatgc
     4501 ggcccggttt accggtatta atattcgaca gtagcccacc taaaatttgt gactggatgg
     4561 cctgagtaaa gttatctaac gctgagggtg tttcaatacc aaagtcatcg ttatagctcg
     4621 gatctttata agagttttgg gcctgagcgc tatttaataa aaaagcgcca ttatttgggt
     4681 taccaccaaa gtttggatta cggaactgga aagtcatggt tccagcccaa cttaatggcg
     4741 aaataagcat gagtagaact actgcatgtt tgacacgcat tgcagcctcc gaacaatttt
     4801 ttatttagaa ttcatcatgc gccaaatcgt ccgtacttaa taacgcctga tttatctggc
     4861 gacgatttag tgcttcttca gtttgaatca gtgcaaagac gacagttttc tcgaagtctc
     4921 ttttcaacgg gaataaaaaa gtctggaaaa taacatcctg attgaccgtt attgtgatcc
     4981 agcttcccca tcgtgcactg ggcctttcat taatcgttaa gttacccgta tagtcacttt
     5041 cccatttatc actaaaggct cggtaaaaat catggccaat agatgaaaca gtatggtcag
     5101 ttaacaatcc cgggacttct acctcaacgg cgtgaagatt ccctgcggcg aacagaaatt
     5161 ctgccgccac aatccagcgt aaataacgtt tcatggcttt atcgcctgag gttatcgttt
     5221 gcccaggaaa ccgcttgtgt ccggtttttt acggctatct tcttgaaaag attataaaga
     5281 tgcgttttaa ccgtattttc gctgatgaac aacgaacgag cgatctcgtt attagacgcg
     5341 ccgatacgca gcttattcag gatctctttt tcccgatgag taaggagggc tgattccgtg
     5401 ctgttataac gatagttacc agaatgtgta atcaggtaac tggcaagctt ttgcgtaaag
     5461 tagcattcgc cacgcaggac gccttgcaac ccattgacaa cacgttcttg atcctccatg
     5521 gcataaaaaa cgccgttgat atgaggccag ttttcaatgt cgcggtacgg gtaatcttca
     5581 ggcgtattta gcaacaatat tttgatattg ttgtttttcc tgctcaaagt atcctgccaa
     5641 taatggataa gctttttatc cgcttccatc atatccagaa gaataataga gcctgaagag
     5701 atatcgtcca gagaacgttg aatattatgt aattttcctg taattgctag cgattgttta
     5761 aggtgctgca agagagctgt cgcctgcaaa gaaggtttag tgatcaacaa taatgtatga
     5821 ccatgaatac tatggacttc attaaacatg atgaaacccc acttttttat tgatcgcaca
     5881 cctgacagct gcctctaaaa tagaagcacc agaagtactg acagatgttg cactgctgtg
     5941 tgtagtaata aatcagccct aaatgggtaa aatataaaac taatggatta catctgattt
     6001 caatctagcc attacaaatc ttaaatcaag tgttaaacat gtaacaaaat gtaactcatt
     6061 atattaaaat gttaacatta aggttttatt aagtttagaa atgatagaaa agttgtacat
     6121 ttggttttta ttgcacaatt ttaaaaaatc atacaaatgg tgctaactta ctaataatac
     6181 atataaaaat atttcggtgt agtcctttcg tcatgtaaaa cgtttttgtt ttttctccac
     6241 acctccgtgg acaatttttt actgcaaaaa gacgaggttt gtcacggctt gtgcgcaaga
     6301 catatcgcag caatcagcga cgggcaagaa gaatgactgt ctggtgattt ttgatagcgg
     6361 aaaacggaga tttaagagaa aacaaaatat tttttgcgta gataacagcg tatttacgcg
     6421 ggttttaata ctttggtatg aacaaaaaaa gaaaaataca acgcgcgggt gagttattaa
     6481 aaatatttcc gcagacatac tttccatcgt aacgcagcgt taacaaaata caggttgcgt
     6541 taacaaccaa gttgaaatga tttaatttct taaatgtacg accaggtcca gggtgacaac
     6601 atgaaaaaca aattgttatt tatgatgtta acaatactgg gtgcgcctgg gattgcagcc
     6661 gcagcaggtt atgatttagc taattcagaa tataacttcg cggtaaatga attgagtaag
     6721 tcttcattta atcaggcagc cataattggt caagctggga ctaataatag tgctcagtta
     6781 cggcagggag gctcaaaact tttggcggtt gttgcgcaag aaggtagtag caatcgggca
     6841 aagattgacc agacaggaga ttataacctt gcatatattg atcaggcggg cagtgccaac
     6901 gatgccagta tttcgcaagg tgcttatggt aatactgcga tgattatcca gaaaggttct
     6961 ggtaataaag caaatattac acagtatggt actcaaaaaa cggcaattgt agtgcagaga
     7021 cagtcgcaaa tggcaattcg cgtgacacaa cgttaattac cattcgactt ttaaatcaat
     7081 ccgatggggg ttttacatga aacttttaaa agtagcagca attgcagcaa tcgtattctc
     7141 cggtagcgct ctggcaggtg ttgttcctca gtacggcggc ggcggtaacc acggtggtgg
//
LOCUS       DAOFMX010000150         7183 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.151, whole genome shotgun sequence.
ACCESSION   DAOFMX010000150 DAOFMX010000000
VERSION     DAOFMX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.151"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(296..1198)
                     /locus_tag="QBA20_003993"
     CDS             complement(296..1198)
                     /locus_tag="QBA20_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDQ6294335.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="QBA20_003994"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="QBA20_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDQ6294336.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="QBA20_003995"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="QBA20_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDQ6294337.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="QBA20_003996"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="QBA20_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDQ6294338.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="QBA20_003997"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="QBA20_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDQ6294339.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKELLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="QBA20_003998"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="QBA20_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="QBA20_003999"
     CDS             7090..>7183
                     /locus_tag="QBA20_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HDQ6294340.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1673 a         1814 c         1877 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacacgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgag catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgaaccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg ttcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agttgctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAOFMX010000151         6795 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.153, whole genome shotgun sequence.
ACCESSION   DAOFMX010000151 DAOFMX010000000
VERSION     DAOFMX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.153"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            240..2672
                     /locus_tag="QBA20_004000"
     CDS             240..2672
                     /locus_tag="QBA20_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDQ6294341.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="QBA20_004001"
     CDS             2739..4208
                     /locus_tag="QBA20_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDQ6294342.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="QBA20_004002"
     CDS             4208..5869
                     /locus_tag="QBA20_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDQ6294343.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="QBA20_004003"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="QBA20_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HDQ6294344.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6795)
                     /locus_tag="QBA20_004004"
     CDS             complement(6478..>6795)
                     /locus_tag="QBA20_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294345.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1611 c         1554 g         1627 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagt caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataa
//
LOCUS       DAOFMX010000152         6643 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.154, whole genome shotgun sequence.
ACCESSION   DAOFMX010000152 DAOFMX010000000
VERSION     DAOFMX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.154"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..926)
                     /locus_tag="QBA20_004005"
     CDS             complement(<1..926)
                     /locus_tag="QBA20_004005"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDQ6294346.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENSWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENA"
     gene            complement(1350..3041)
                     /gene="fruA"
                     /locus_tag="QBA20_004006"
     CDS             complement(1350..3041)
                     /gene="fruA"
                     /locus_tag="QBA20_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDQ6294347.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(3058..3996)
                     /gene="fruK"
                     /locus_tag="QBA20_004007"
     CDS             complement(3058..3996)
                     /gene="fruK"
                     /locus_tag="QBA20_004007"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDQ6294348.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(3996..5126)
                     /gene="fruB"
                     /locus_tag="QBA20_004008"
     CDS             complement(3996..5126)
                     /gene="fruB"
                     /locus_tag="QBA20_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDQ6294349.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            5493..>6643
                     /gene="setB"
                     /locus_tag="QBA20_004009"
     CDS             5493..>6643
                     /gene="setB"
                     /locus_tag="QBA20_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDQ6294350.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
BASE COUNT         1550 a         1854 c         1593 g         1646 t
ORIGIN      
        1 gcgttctcca ctaacgatat aacgttggca atcgataaat cggggttatt ggtgtattca
       61 caggagagcg ccattgacga acatccctgt gcgaaacgaa cagattcggc aaacggcatt
      121 ccgtctaccc aacacgaagc aagtcccgcc atcatggcat cgcccgctcc ggtaacatta
      181 ataacattgg ttttgatcgg cgcagaccag ctattttcac cgctgatatc gctgtaataa
      241 acgccgtcgc cgcccatgct caataccagt cggttcaggc catgttgatg gaaccaggca
      301 gcaacttttg ccacatcgtc acgccctgac agcgcaatcc cactcagggt ttccgcttca
      361 aggcggtttg gcttcagagt gtggatctga tttagacggt cgcgcacttt gacacatttc
      421 cgcgctgaaa ccggatcgac aaatacgggt acgttggcgg cattatccag aatccatgcc
      481 agtgcctctt cactgatatt acagtccgcg acaatgacct ttgccctctg aataaattca
      541 cggtgctgtg cgagatattc agctgtaata gcgttgctaa tattcatgtc atttatagca
      601 accagcattt caccggtatt atcgagtaat gataaataac tcgacgtatt ttctcccggc
      661 acaatcaggc atttatcgac ataaacacca gattgattgg tttgcgttag cagcgattga
      721 ccataaaaat cactgcctac ggcgctcagt agccaggctt tgttacccag caacgccagg
      781 ttttgtgcaa tattgcgccc tactccacca ggcgtaaatt ttattttacc tggatttgaa
      841 tccgcataat ttaatgattc atgtgaatat ccggcgacat caatattcgc cgaacctata
      901 attacgacat aatccttttc gcgcatagca cgtcctctgg taaaaaatta ccaaccctgt
      961 caatccacaa gaatatagat actgcaaaga cggtttaaac atgtgttcat atttaaacat
     1021 gtgctcatag tatgcatgtt ttacgtaaag taaattaccg ccagagaggc aaaatgggca
     1081 gatttgagca ggctcacaaa atgcgattaa atgcctgaat tacaaaagcg aggaatttgt
     1141 gacgggatgc acagaacagg aacactaaaa aagggcagaa aataatctgc ccttgaggaa
     1201 ataaccttag tgtttgatga acgtttgaag tttctactga aagataaaac catttaaatt
     1261 cataaaatta cattcacaag ctggtcattc tctttgcgct catcaaatgt tacagggcag
     1321 gaaatttctg ccctgtaaca caccttttat tacgctgctt tcgctactgc gtccacttcc
     1381 ggacgtttca ggaaggcata ggccaaaccc gccaccagcg taccggcaat aattgctacc
     1441 aggtaaccca ataccggcgt aatagcgcca gggatcagca gaacaaacag accaccgtgc
     1501 ggtgccatca gtttcgcacc aatcgccatt gagattgcgc cagtcagcgc cccacccacg
     1561 atacagcacg gcagcacacg catcggatca cgagcagcaa acggaattgc accttccgaa
     1621 atgaagcaca gtcccaatac cagagcggct ttgccacctt cctgctgcgc tttgtcgaat
     1681 ttgcgacgcg ccaccattgt tgccaggccc attgccagcg gtggcaccat acctgccgcc
     1741 ataatcgccg ccatcgggcc ataggtttga gtactcagca gacccacacc gaatgcgtac
     1801 gctgctttgt ttaccggacc gcccatgtca gtacacatca tgccaccgag gatcgccccc
     1861 agcagaaccg cattcgcagt ccccatggtc tgcagccagt gagtcagccc ttcgagaatg
     1921 ccagcaactg gtttaccgat caggtagatc atcgccagac cgaccaccag actggaaatt
     1981 agcggaatga tcaggatcgg tttcagcgcc tccatactct gcggcagttt cagttgcgta
     2041 ctgattaact tcgcaatgta accagccagg aagcccgcaa taataccgcc aatgaagcca
     2101 gaaccggtgc tgaccgccag cataccgcca atcagacccg gcgtgaggcc cggacgatcg
     2161 gcaatggaga aggcaatata acctgccagt accggcacca tcagcgcaaa ggctgaacca
     2221 ccaccaatct gcatcagcgc cgctgccaac gtacccggct ctttaaacgc ttcgatacca
     2281 aaagcaaaag aaagcgcgat acacagacca ccagcaacga ccatcggcag catgtaagag
     2341 acgcccgtta gcaagtgacg ataagcgcct gcactctctt tcttaccttc agtggtcgcc
     2401 gtttgagctt tgcccgccgg ttcatacggc gttgcttcag caaccgcttt atccagttcc
     2461 tgcgaggttt tcttcagcgc cagaccggta gaggtacgat acatcggttt accagcaaat
     2521 ttcgccagat ccacttcgat atctgccgcc acaatcacca gatccgctgc ggctacttcc
     2581 tcgggagtga ttgcattacc cgcgccaaca gaaccacggg tttcaacttt cacccaccag
     2641 ccacgttttt tcgcttcggt ttcaatggct tcagccgcca taaaggtgtg tgctacgcca
     2701 gtcgggcaag cagtcaccgc aactacgcgt ttcggaccgc tggcggcaac cggtgctgtc
     2761 gcagtgaccg gcgcagtgta aggtttcgca tggcctttgg cttcactcag gaacagctca
     2821 gggtgcgcaa ctgcccggga aatatcaccc agccagacat ttttaccgtt cagcgcgctg
     2881 tcattcggga tggaatcacc gagaacaatc gccatttctg cgtcgttcgg attgtcgatg
     2941 atttccagtt ttgcttttcg cgccgccgcg cccagcaggg tcttcgccat ataggcgcgt
     3001 gcctgaccga gattagcgtc aataatcagc agcgttttca ttatgcctct cctgctgtca
     3061 gttaaaaggt tgtaagtcga cgcgcgccat cattgcggcc aactgcggac gatcggtaat
     3121 acccacattg ctttgactta ccgccagggc tgcaacagct gtcgccagac gcagtgtgtg
     3181 ttcactggat tcacgcatca gcaagccata aatcaggcca ccaaccatag aatcccctgc
     3241 gccaacggtg cttacgacat cgactgacgg tggtttagcg atccattcgc cggaggcatt
     3301 aacccaaagc gcgccttcgg cacccagtga aataacaaca tgcgcaatac cttgttcacg
     3361 tagcgcatgt gcagcttcaa tcacatcttt catttcaggc agtttacggc ctgcccagat
     3421 ttccaactcg cggcggttag gtttcaccag ccacggtgcc gctttcaaac ctgctactaa
     3481 cgcttcacgg ctactatcaa agataatgca aggacactga ctacgcaggc gagtcatcca
     3541 gtcggtgaac gcttccgggc tgacgcctga cggtaagctt ccgctgacac agaccatatc
     3601 gaactgaccg agccagctca gagaatcagt cacaaagcgt tcccagtcgg cgggggtgac
     3661 ttcgaaaccc gagaagttga agtcggtcac ttcgccgtct ttttccgtca gcttaacgtt
     3721 aattcgggtg cgcccctgta caacctggaa acggttggca atgcccagct cgctgaacag
     3781 ttgctgaaaa ccatcctgat tgtctttacc taggaagccg ccaacggtga catcaattcc
     3841 caggtctttt aatactttgg ccacgttgat gcctttaccc gccgcatgca gaccggtggt
     3901 tttcaccagg ttcacttcgc cgcgttcaat ttccgggcag aaaccaacaa ggtcataagc
     3961 cggattaagg gtgatcgtag caacacgtct gctcattatg cgccctcccc aagaccagca
     4021 gcgatagcgt cgccaatggc tttcagcgcc tgttcagcat ctgcaccctg ggcggtaaag
     4081 cgtaggcgat gacctttctt aacgccaagt gccacaactt tcatcagact acgtccgttt
     4141 gccggtttgc cggtgccatc aaggtttgtc acggtaatat cactgttaaa ttgtttaatg
     4201 gtattgacca gcatggtacc tggacgagca tgcaggccgt gttcattgcg caccacaaac
     4261 tccgcgctta acacgtcgtc ggtcggcgca tcatcgctgg tcagcagcgc cagcaacgtt
     4321 gccgcatccg ctttcagcaa gcggtcagct ttattgtcga gcaacaaatc agcgagacgc
     4381 ttaagaaccg cgatgggctg atcgtcattc atcgccacac tcaccagcat ggctgccgtt
     4441 tcgccgtcca catcaaaagc atttgccgca cggcttaccg caatcgcact acgcagattg
     4501 ccttcggcgc tatcgctcag ccagataccc tgtccgagat tcagcggttg ttcattgatg
     4561 gctttggtga cgaaagtggc gtcaactgcc ctcgcctctt tcagacgcgc agcgttcagc
     4621 gcctgaagag tcagcagatc gctggcgacg atatccagcg tcagcatttc gttgtcgagc
     4681 ttcagctgct cactctgctt ttcgcccatc agtaatgcgc gaagttcttc tgctgttgtt
     4741 gctgacttca gttgttcagc aacggaatca tcgctcagta cgtgggtcag ctggcgtagc
     4801 aggcccagat gtttatccga gctggcagca ataccgattg ccacgtacgc tacctgaccg
     4861 tcaccccagg tgacgccttc cgggaactga aatacctgaa cgccggtttt cagcacctga
     4921 tcgcgggtgt cggtagtgcc gtgtggaata gcaataccat tgccgaggaa cgttgaggtt
     4981 tgctgttcgc gcgccagcat gccattgacg tagccttctg ctacattacc ggcctgcacc
     5041 agcgccgcag cgacctggcg aatcgcctct tctttgtctc cggccttttc gcccggatgg
     5101 atgtcctgta cggataactg gaacatagtt ctcctctctt gctgaattga aacgattcag
     5161 cctctatgag aaaaaaagcg ccaacctagc ttagggttaa agacaagatc gcgctgaaac
     5221 gtttcaagaa agcataatac ttctgtttca gcacgcaagg aaagccgtaa agttatgagc
     5281 aaaagtttga tgtgctgcac aattttttgt catttttcct aattgctgat gggaaaatcc
     5341 gttatcagca atttcatttc agcagcgtca gcagccaatc agcaacagtg aaatggcatt
     5401 ttgattattc gtggcgcttt tgatttcgct gtctgtttat tttctgacaa gcagcgtaaa
     5461 ctcgcgtctt cctcttccag tgatcgacca gcatgcataa ctcccccgca gtctccagcg
     5521 cgaaatcgtt tgacctgacc tcgacggcgt ttttaatcgt tgcctttctc accggtattg
     5581 cgggtgctct gcaaaccccg acactcagta ttttccttac cgatgaagta catgcccgtc
     5641 cggcgatggt gggattcttc tttaccggca gcgctgtcat tgggattctg gtaagtcagt
     5701 ttctcgctgg gcgctctgat aagcgcggcg atcgcaaatc gctgattgtc ttttgctgcc
     5761 tgttaggcgt gctggcctgc accctttttg cttggaatcg caactacttt gttttgctat
     5821 tcgttggcgt ctttcttagc agctttggct cgaccgctaa cccgcaaatg tttgcccttg
     5881 cccgtgaaca tgccgacaaa accggacgtg aggcggtgat gttcagctct tttttacgcg
     5941 ctcaggtttc actggcatgg gtcattggcc caccgctggc ttatgcgtta gcgatgggtt
     6001 tcagctttac ggtaatgtat ctgagcgcag cggtagcgtt tattgtttgc ggcgtgatgg
     6061 tgtggctgtt tttaccgtcg atgcaaaaag agcttccgct ggcgaccggc acggttgaag
     6121 cgccgcgccg taaccgtcgc gatacgctac tgctgtttgt catttgtaca ttgatgtggg
     6181 gctcgaacag cctgtacatc atcaacatgc cgctatttat tatcaacgaa ctgcatcttc
     6241 ccgagaaact ggccggtgtg atgatgggga ccgccgccgg gctggaaatc ccgacgatgt
     6301 tgattgccgg atatttcgcc aaacgtctgg gtaagcgttt cttaatgcgc gttgctgccg
     6361 tgggtggcgt ctgtttttac gcaggaatgc tgatggcgca ttcacctgcc attctgttgg
     6421 gcttgcagct gctaaatgct atttttattg gcattctggg cggcatcggg atgctctatt
     6481 ttcaggattt gatgcccggt caggcaggtt cagccaccac gctctatacc aacacgtcgc
     6541 gcgtgggctg gatcatcgca ggatcagtgg cgggcatcgt cgccgagatc tggaattatc
     6601 acgctgtgtt ctggtttgcg atggtgatga ttatcgccac tct
//
LOCUS       DAOFMX010000153         6522 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.155, whole genome shotgun sequence.
ACCESSION   DAOFMX010000153 DAOFMX010000000
VERSION     DAOFMX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.155"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            441..1829
                     /gene="narU"
                     /locus_tag="QBA20_004010"
                     /pseudo
     CDS             441..1829
                     /gene="narU"
                     /locus_tag="QBA20_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
     gene            1911..5651
                     /gene="narZ"
                     /locus_tag="QBA20_004011"
                     /pseudo
     CDS             1911..5651
                     /gene="narZ"
                     /locus_tag="QBA20_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5648..>6522
                     /gene="narH"
                     /locus_tag="QBA20_004012"
     CDS             5648..>6522
                     /gene="narH"
                     /locus_tag="QBA20_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDQ6294351.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPSWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1557 a         1715 c         1692 g         1558 t
ORIGIN      
        1 accttagatg aaataaacaa tacttactat aataacgatt attacaatca ggcaataaga
       61 gaggaaccga tagactttct ggacagatcg ttttcctcca attcatggcc tttttagtga
      121 ataaatcatc actaattcta taagtgtctg gtcttgttga gaatatagtt aatatgtaac
      181 cctttagatt cacgatctga agggttgaaa catgacatac gttcaaagat taacccccgt
      241 ttgaaggtta gcttgttagt tacatttagt aacacatatc tggctactta atccccattc
      301 tgcccccgcg gttacgcttt cttatctatt ctttcaatca gcacttcacc caattcatcc
      361 ttaattctaa taactggcta ttgcgcctta cctcctgttt tgtctttttt cattaccaat
      421 gtgtgcatat gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac
      481 gcgactggaa accagaaaat ccggccttct gggaaaataa aggaaaacat attgctcgaa
      541 gaaacctctg gatatcagtc agttgtctac ttcttgcctt ctgtgtctgg atgctattta
      601 gcgcagttac cgttaatctc aataaaatcg gttttaattt tactaccgat caactctttt
      661 tattaaccgc attaccctcc gtttctggcg cattattgcg tgttccctac tcctttatgg
      721 tgcctatatt cggtggacgc cgatggccgg tttttagtac tgcaatcctg attattcctt
      781 gcgtctggct cggaattgcc gtgcaaaatc cgaatactcc ttttgggata tttatcgtta
      841 tcgctttgct atgcggtttt gcaggtgcaa actttgcttc gagcatgggc aatatcagtt
      901 tcttctttcc aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact
      961 taggtgtaag tgtaatgcag ctggttgcac cgctggtcat ttttgtacct gtatttgcct
     1021 ttctcggcgt caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg
     1081 catagatttg ggtaccgcta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg
     1141 atatcgccag ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc
     1201 tctggctgct gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg
     1261 gttttgccat gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct
     1321 ttggcccatt tattggtgcc atcgcgcggt cggttggtgg tgctatttcc gataaattcg
     1381 gcggcgtgcg ggtgacgttg atcaacttta tttttatggc gattttcagt gccctgctgt
     1441 tccttacctt accgggcaca ggctccggta atttcatcgc cttttacgcc gtatttatgg
     1501 ggctgtttct gaccgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct
     1561 ttcgccagat aaccatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaga
     1621 gagaagccgt caccgaaacg gcggcaacgc tgggctttat ctcagccatt ggcgcagtgg
     1681 gcggcttttt tattccgcag gcatttggca tgtcgctcaa tatgaccggc tctccggtcg
     1741 gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt gctgctgacc tggctggttt
     1801 atggtcggcg gaagttcagc caaaaataag agatcgatgc ctgatgtaca accatgtcgc
     1861 atccgacaag gcatcagatc ccaattcatt cctggagcag gagtcatgtc atgagtaaac
     1921 ttttggatcg ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac
     1981 aggtgatgca tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca
     2041 aaatcgtgcg ttccacccac ggcgttaact gtacaggctc ctgtagctgg aaaatctacg
     2101 ttaaaaatgg tctggtgacc tgggaaatcc aacagaccga ctacccgcgc actcgccctg
     2161 acctgcccaa tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc tggtatcttt
     2221 acagcgctaa ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa ctgtggcgcg
     2281 aagccctcaa acaacacagc gatccggtac tggcgtgggc atcgattatg aacgatccgc
     2341 aaaagtgcct gagctacaaa caagtgcgtg ggcgcggcgg gtttatccgc tccaactggc
     2401 aggaactaaa ccagctgatt gccgccgcta acgtctggac catcaaaacc tacggcccgg
     2461 atcgcgttgc cggtttctcg ccgatcccgg cgatgtcgat ggtttcttac gccgccggaa
     2521 cgcgttatct gtcgctgctt ggcggcacct gttaaagttt ctacgactgg tattgcgacc
     2581 tgccgcccgc ctcgccgatg acctggggcg agcaaaccga cgtaccggaa tctgccgact
     2641 ggtataactc cagctacatc atcgcttggg ggtctaacgt accgcagaca cgtacgccgg
     2701 acgcccactt ctttaccgaa gtacgctaca aaggcactaa aaccatcgcc attacccctg
     2761 actactctga agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata
     2821 gcgccctggc gatggcaatg gggcatgtga ttttaaaaga gtttcatctc gataatccca
     2881 gcgactactt tatcaactac tgccgccgct acagcgacat gccgatgctg gtaatgctgg
     2941 agcctcgcga cgatggtagc tacgttcccg ggcgcatgat ccgcgcatct gacctggtgg
     3001 atggactggg cgaaagcaac aatccgcagt ggaaaaccgt agcagttaat accgcaggtg
     3061 aattggtagt gccgaacggt tcgattggtt tccgctgggg agaaaaaggc aaatggaatc
     3121 tggaatccat tgccgccggt acggaaaccg aattgtcgtt aaccctgctc ggtcaacatg
     3181 acgctgttgc aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca
     3241 gcgtaaaaca caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg
     3301 atggcaacac ctgtccggtg gtcagcgttt atgatttggt actggcgaat tacggcctcg
     3361 atcgcgggct ggaagatgaa aacagcgcga aagattacgc tgaaatcaaa ccgtacaccc
     3421 cagcctgggg tgagcaaatt accggcgtac cgcgccagta tattgaaacc atcgcccgcg
     3481 aatttgccga tatcgcccat aaaacgcatg ggcgctcaat gattatcctc ggcgcaggtg
     3541 ttaaccactg gtatcacatg gacatgaact accgcgggat gatcaatatg ctgatcttct
     3601 gcggttgtgt cgggcaaagc ggtggcggct gggcacacta tgtcggccag gaaaaactgc
     3661 gcccacaaac cggctggttg ccactggcct ttgcgctcga ctggaaccga ccaccgcgcc
     3721 agatgaacag cacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct
     3781 ctgctcagga gttactttca ccgctcgccg atgccagtaa gtacagcggt catctgattg
     3841 atttcaacgt tcgcgccgaa cgtatgggct ggctaccttc tgcgccgcag ctggggcgta
     3901 acccgctcgg gattaaagct gaagccgaca aggccggatt atcccccaca gaatttaccg
     3961 cccaggcgct gaaatcgggc gatttacgta tggcctgcga acaaccagat agcggcagca
     4021 atcatccgcg taatttgttt gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc
     4081 acgagtatat gcagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg
     4141 gtgccagcga cgggatcaaa ccggaagaag tcgagtggca aactgcggcg attgaaggca
     4201 agctcgacct gctggtgacg ctcgacttcc gcatgtccag tacctgcctg ttctccgata
     4261 tcgttctgcc caccgccacc tggtatgaaa aagacgatat gaacacctcg gatatgcatc
     4321 catttattca tccgctttct gcggcggtcg atccggcctg ggaatcacgc agcgactggg
     4381 aaatctacaa aggtattgcc aaagcatttt cgcaagtgtg cgtgggccat cttggcaaag
     4441 aaaccgacgt ggtattacaa ccactgctgc atgactctcc ggcagagctc tcacagccgt
     4501 gtgaagtgct cgactggcgc aaaggcgaat gcgatctgat cccgggtaaa accgcgccga
     4561 atattgtggc ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc
     4621 cattgatgga caaacttggc aatggcggta aagggatttc gtggaatacc caggatgaaa
     4681 tcgatttcct cggcaaactc aattacacca agcgtgatgg cccggcacag gggcgtccgc
     4741 tgattgacac cgccattgac gcttcagaag tgattctggc actggcaccg gaaaccaacg
     4801 gtcatgttgc agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc gaacataccc
     4861 atctggcgct gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc
     4921 gtaaaattat ctccagcccc acatggtccg gtctggaaag cgatcacgta tcctataacg
     4981 cgggatacac caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc
     5041 tctatcagga tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc
     5101 ctatcgacac ccgtagcgtc agtgagatgc gccagatacc gccaaacggc ttccaggaaa
     5161 aagcacttaa cttcctgacg ccgcaccaga aatggggcat tcactcaacc tacagtgaaa
     5221 acctgctaat gctgacgctc tctcgcggtg gaccgattgt ctggatcagc gaaacagatg
     5281 cccgagaact gaccattgtc gataacgact gggtggaagt atttaacgcc aacggcgcgc
     5341 tgactgcccg cgcggtggtc agccaacgtg taccgccggg catgaccatg atgtatcacg
     5401 cccaggaacg cattatgaat attcctggtt cggaagtaac tggcatgcgc ggcggcattc
     5461 ataactcggt cacccgcgtt tgcccgaaac cgacgcatat gattggcggt tacgcgcagc
     5521 tggcctgggg ctttaactac tacggcaccg tcggctcgaa ccgcgacgaa ttcatcatga
     5581 tccgcaagat gaagaacgtt aactggctgg atgatgaagg tcgcgatcag gtacaggagg
     5641 cgaaaaaatg aaaatacgtt cgcaagtcgg catggtgctt aacctcgata aatgtatcgg
     5701 ctgccatacc tgttcggtga cctgtaacaa cgtctggacc ggacgcgaag gcatggagta
     5761 cgcatggttt aacaacgtcg aaaccaaacc gggcattggt tatccgaaaa actgggaaga
     5821 tcaggaagag tggcaaggcg gctgggtccg cgatgtgaat ggcaagatcc gtccgcgtct
     5881 gggcagcaag atgggcgtaa taaccaaaat cttcgccaac ccggtggtgc cgcagcagat
     5941 tgatgattac tacgaacctt tcaccttcga ctacgaacat ttgcatagca caccggaagg
     6001 caaacatatc cctactgctc gcccgcgttc actgattgac ggcaaacgga tggacaaagt
     6061 gatctggggc ccaagctggg aagaactgct gggcggcgag tttgaaaaac gtgcccgcga
     6121 ccgcaacttc gaggccatgc aaaaggagat gtacgggcag tttgaaaaca ccttcatgat
     6181 gtacctgccg cgcctgtgcg aacactgcct caatcccagc tgcgtggcga cctgcccaag
     6241 cggcgctatc tacaaacgcg aagaagacgg cattgtgctg attgatcagg ataaatgccg
     6301 tggctggcgt ttgtgcatta gcggttgtcc gtacaaaaaa atctacttca actggaaaag
     6361 cggcaagtca gaaaaatgta tcttctgtta tccgcgaatt gagtcaggcc aacccaccgt
     6421 gtgctcagaa acctgcgtgg gtcgcatccg gtatctgggc gtgctgcttt acgacgccga
     6481 ccgcatcgag gaagcgtcga gcaccgagcg cgaagttgac ct
//
LOCUS       DAOFMX010000154         6392 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.156, whole genome shotgun sequence.
ACCESSION   DAOFMX010000154 DAOFMX010000000
VERSION     DAOFMX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.156"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(142..522)
                     /locus_tag="QBA20_004013"
     CDS             complement(142..522)
                     /locus_tag="QBA20_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDQ6294352.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(515..715)
                     /locus_tag="QBA20_004014"
     CDS             complement(515..715)
                     /locus_tag="QBA20_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HDQ6294353.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(810..1067)
                     /locus_tag="QBA20_004015"
     CDS             complement(810..1067)
                     /locus_tag="QBA20_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294354.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(1064..2455)
                     /locus_tag="QBA20_004016"
     CDS             complement(1064..2455)
                     /locus_tag="QBA20_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDQ6294355.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(2452..3330)
                     /locus_tag="QBA20_004017"
     CDS             complement(2452..3330)
                     /locus_tag="QBA20_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDQ6294356.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(3341..4249)
                     /locus_tag="QBA20_004018"
     CDS             complement(3341..4249)
                     /locus_tag="QBA20_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ6294357.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(4236..4469)
                     /locus_tag="QBA20_004019"
     CDS             complement(4236..4469)
                     /locus_tag="QBA20_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294358.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(4466..5482)
                     /locus_tag="QBA20_004020"
                     /pseudo
     CDS             complement(4466..5482)
                     /locus_tag="QBA20_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            5414..5677
                     /locus_tag="QBA20_004021"
     CDS             5414..5677
                     /locus_tag="QBA20_004021"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDQ6294359.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            5667..5975
                     /locus_tag="QBA20_004022"
     CDS             5667..5975
                     /locus_tag="QBA20_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6294360.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(6017..6193)
                     /locus_tag="QBA20_004023"
     CDS             complement(6017..6193)
                     /locus_tag="QBA20_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HDQ6294361.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
BASE COUNT         1560 a         1723 c         1483 g         1626 t
ORIGIN      
        1 gtttaacgtc ctgtaccgtt tttcaggcat aaaaaacccg cccggaggcg ggtttaagct
       61 gtgtggcgaa gcaaccactc ttaacagaat atcccgtttt ttacgtacgt aaaatatttt
      121 ctagaaagtc gccccttacc atcagggatg ttcaatatat ttgtccattt ctaaccggac
      181 acccaacatc atcaacattc cttccaccac cccctcggct ttttgtagct tctttcctat
      241 ataaccatct gagcatccat gcttccgcgc cagcgtcatg aatgtcatcc caaacacgta
      301 gtagtcaacc agcaagtcat gtagatcgtt gttgttccgg ttaaggcggg ccatacaccc
      361 acatataacc atcgcgtcat cgtcacagca ttgcgggcgg gattttactt ttgaagggat
      421 taatccctta aaaccggcag caatggacga ccaggtcaca tcctcatgat tattagccac
      481 ccacgcgccc cagcgctcaa gaacctgctg aatatcacgc atcactgtct ttacccctgt
      541 cccatccacg atgaacaatc agaacaccat caacaatggc gtgccctttg ccctctttat
      601 cttcagcata ttttcttacc gtggcgcgat tacagttcag cattcgtgaa acttcggtca
      661 tattacctcg tgtctggata agcagttccg gtatcgtttg aattttgacg ctcatcaaat
      721 actctccggt tcggtgattt ttatcccggg ctttccacca gggacataat gatcgcgcac
      781 aagccagggc aaaacaaact catggcgcat cagcgcagcg cctcctgcac cagtttttca
      841 aactttccga ctctggtttc cagctctgcc acacagtcca ccagctcatc tactgctttc
      901 tgtgcgcgat gcttcgcctg catcagttcc cgaagcgcgg gtaccatatc tttacggata
      961 gcatcttttg ttatgcccgt tttttcgagt tgttcagcat gacgcagcat ttcctgcgcg
     1021 tgtttacgca attgttcagg ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca
     1081 tcttactgct gtcagttcgt ttattactgt atctgcgcgg attgccgggc ttcatgggag
     1141 tggaaagcac ccgtgcactt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc
     1201 ggtaaatcgt tcccagagaa ccgttacgtt gtttcgtgat attgatttct gcgatgcccc
     1261 tggcctgcgt atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat
     1321 ccgcctctat ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg
     1381 actccacacc gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca
     1441 ggcctttaag cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg
     1501 tttttatcag cccgagataa tcaaccacca ccagcgccgt ttccggatat ttaatcaggt
     1561 ggtgtttcgt tgttgcgcat atctcatcaa tggccaggtt cgcctggtcc accatccaga
     1621 tattgcgccc ggtcatccgc cccacccctt gtgagaaacg cgtccagtct tcatcttcaa
     1681 agtgagccac agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt
     1741 caccaatctg gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag
     1801 tcaccttgtc gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa
     1861 tgaataccag gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg
     1921 tcatcaacgt cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt
     1981 ccggaagcac atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga
     2041 ccatcatgtt ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac
     2101 gtaatttctg cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc
     2161 gcaacgctgc aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag
     2221 taatcaccgg ggcaagttcc ggcatctcat tacacaacag cacggggtca atcactcctg
     2281 aaacacgggc ctgtctgcag atgcctgtat agatatcctg atacgctcgt acagaaaaaa
     2341 cgtccgctgg cagtgtggcc agaatatcca tcacttcagg atctgctccg cgcagaaaga
     2401 acgcgccgat gacagcgcct tcaaggtcat cgttacgcca tactggagtt gtcatgcagc
     2461 cacacctctg atacgagaac ggtaactggg ccagttaaac gacaaccagt tgcgtccccc
     2521 gtctgtgatc ctgtcggcaa ttcggggact gatgaacgcc cacaactctt ccggtgagag
     2581 gttactgatc agaatggtgg gcaagatact ttcgtacctg gcgttgataa tttcctgcaa
     2641 aatagccatt tcagccgcgc tgccaaactg aacgccaact tcgtcgatga tcagcaaatc
     2701 cattgacgca taacgctcaa taacttcatc cgctgttttt tcgctgtcat tccgccagca
     2761 atttttcaca gcacgggtaa ggcgcatcac gtcggtgatc tccacactgg ccagatagtt
     2821 acggatgatg tgttttgcca tagccacagc cagatgattt tttccggtac cacaactgcc
     2881 ggtcataaca agactggtac cgttctccag catatctggc cagttctccg catagcggcg
     2941 acaggccgca agatttctgg ctgcgccagg attaacctcc agataattat caaactcgca
     3001 gtcccgaaaa cgcagggcaa ttccggcgtt atcagtcagc tcttccgcct tgatggacga
     3061 cagctccatg gtcaaatcgt tggcctcagc gattaagcag tcagggcagc atgaaatttc
     3121 ttctctgtcc tcgccattac gatcgctcca caccagtata tgcgtgtgat attcgccatg
     3181 tttttcgcaa tatccgcgac cttcacgcat caggcaggaa cgataaggcc atggcttttc
     3241 gcccttctga gcaaatgcaa tctctgcccg taactcatcc attcgcgcct gtagtctttt
     3301 ttgttgttca cgcaggttaa acgtcatcat cgctgtcacc tcagaatgtc agtttgtcac
     3361 tggatttacc gaatttgtca gacatggctc ccaggccagc caggacatcg acctgtcgct
     3421 gtcgcccacc tctgggagcg gctggctgtt gccagaagtc ttcgaagtga cgatcgggtc
     3481 caaagaacgt cgcagcctgc ttcacgaact gtgtgccggt atttcctgtg gcacgtaccc
     3541 aggcagcata gcgtttcacg ccatcaagca tggcttcagg ttttattccc tccctgagcc
     3601 gggctttcca ggctttgaag gctgcggatt ttgagttacc accagcacgt tttggatatt
     3661 cctgccaggc ctgttcaaat tccggtgaat attcctgtcg ggaagaacgc gctggtgcag
     3721 acgcgtcagc ggatgcgcca atagtgtttt tactctctgt agtattctct gaagtaatct
     3781 ctgttgtatt ctctgtaaga tcgaaattgg ttttcccttc tccgcggcga ggggtttccc
     3841 gtgtccgcgg tgaaggcttt ccctcctccg cgaaattggg ttttacagtt tcccgaaaac
     3901 gggtttcccc atttcgggaa aactgattgt tttcattgat aatttcatta aggcgctcac
     3961 aatctatacg gtagaacatt ttgtgctcaa gacgcttgtt ggtttcaacc aaaatgcctc
     4021 tggacacaag atgcttacgc gctacagcct gttgttcaaa tgtaagtccg gtttcgtgtt
     4081 gtatctcttc acgcgtttta tgtacgcctt ccgctgcatg tgctttatcc tgccagtaaa
     4141 aaatctgacc aaagaaaata acagcgtgcg gacttcccat gtatttaacg agcccagggt
     4201 aataagcaac cggatgtcca aaatcgagca gaagatcaga cggacgcata gccacctccc
     4261 aggcgtttaa acatttttcc gggctgaaac gccaccagcg gataactcag ggtatgagta
     4321 cgtccctgaa cctggcagac aaccttctgg ctttcggtac tgaccaggca aacccgcaga
     4381 acgtagccgt tgctggtggt gaaccactgc cccacacggg ggcaatggtt gtatcggtga
     4441 tacaggaaat taacgatgtg gcggatcatg ggcgcacctc cttgtcagaa ccattcagcc
     4501 tggaatcaac aagtgcagca ccaaaaacag catcacctac acggtcgtac agtttgccag
     4561 ccagcggaga ttcaacggcc ttaagcattg ggtaaagctg gcttgtccag atttgatgga
     4621 tttcacgcaa atgcaggtat acgcctctgg cgttttgtgc gacagatgac atgtcagccg
     4681 caccagctcc tgataagctc ctctccatct ggttaaaggc attgatgtat gcctctttga
     4741 accgggcggt acgtttacca gtgaagccca tagcaaggaa ggcgaagccg ccgcgggtaa
     4801 tttggtagca gggaagtttg cgagtaccgc cgttgggctg gcgtaccaaa attgatgtct
     4861 ccgcaaaatt gcgggcacaa aactctggag aacaatccaa aatgcggatc tttttcagaa
     4921 catcgtcatg acgtttagag aagaagttgg caacagccag ggatgaagta acagcctaac
     4981 catcaacgat ggcaatttca ggttgagaga gggttgggag agtagtcatg gtgacagccc
     5041 ctatgttgaa ttcaaagaac tcaccacatg ggacgccaat cacagaggtg gtgagacgta
     5101 cagggttggc gtaaccggtc aacatagaac ccggcgcatc ttgcgatgtc cctgcacgcc
     5161 ccaccataat ttgggcgtag caatgctcat gacacgaaaa aaccgcatga gcgcggttat
     5221 gctctatatt gaatttcagg acgccaatcc cggcccccgc tttataaggt gcagagacag
     5281 tgtaacgtcc cgaaattgca gaatcaatat attgtgcaga tataacgatt ttgttatgtt
     5341 ttgctgtatg aactacacta tcgaatacta cagtgaagag gtccgactgg aggtcgatca
     5401 gcttccattg agtatgcgtg ccagatacca gcatcttgtt gaacgcatga aggtatacgg
     5461 cagcaatctc ggagaacctc acaccagcgc ctttggtgac gggcttttcg aactcagaat
     5521 taaaggcaac gatgggatcg cgcgtgtttt ttactgcact ctgacaggga aacgcatcat
     5581 catgctgcat agttttgtaa agaaagcgca gaaaacaccg ccagccgaac gcaagaaagc
     5641 tgaaaccaga atgaaggagg ttaagcatga ctggtaaacg cactctcccc accatgacac
     5701 acgacgaaat ggcagccaaa tggatggaag acccggcttt taaagcagaa tacgacgcta
     5761 tcgctgacga attcgcactg cttgatgaaa tgctggcagc acgcaaagaa gctggcttaa
     5821 ctcaggctga agttgccgag cgaatgggaa caaaagcgac cgcgatcacc agaatggaaa
     5881 gtaatctcgc atcaggtatc agcggcccat catttgccac actaaaaaaa ttcgcccgcg
     5941 ctactggaaa aaaactccag atccgcttcg tttaaccaca ccgcgccgtc attctggcgg
     6001 cgcaccggat aaatagtcat ttcctcgcac gatgtcttag ccaccggata tcccacaggt
     6061 gagccgtgta gttgaaggtt tttacgtcag attcttttgg gattggcttg cgtttatttc
     6121 tggagcgttt cgttggaagg tatttgcagt tttcgcagat gatgtcggtg aaacttcgtc
     6181 gatgtcgcct cattcatacc tcctgtcggt aaatctgaca ccctgaccaa tagcccaggc
     6241 tgttgtgtat gaagtggcac actgaatttg gccacctgaa cagaggtgat atgctcacct
     6301 cagaacaaca caggtgctcc aatgaaaaaa agaaatttta gcgcagagtt taaacgcgaa
     6361 tccgctcaac tggttgttga ccagaaatac ac
//
LOCUS       DAOFMX010000155         6385 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.157, whole genome shotgun sequence.
ACCESSION   DAOFMX010000155 DAOFMX010000000
VERSION     DAOFMX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.157"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="QBA20_004024"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="QBA20_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDQ6294362.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="QBA20_004025"
     CDS             complement(1433..1759)
                     /locus_tag="QBA20_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDQ6294363.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="QBA20_004026"
     CDS             1894..2235
                     /locus_tag="QBA20_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDQ6294364.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="QBA20_004027"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="QBA20_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="QBA20_004028"
     CDS             complement(2833..3543)
                     /locus_tag="QBA20_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDQ6294365.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="QBA20_004029"
     CDS             3651..3956
                     /locus_tag="QBA20_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDQ6294366.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4478
                     /locus_tag="QBA20_004030"
                     /pseudo
     CDS             4155..4478
                     /locus_tag="QBA20_004030"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5034..5612
                     /locus_tag="QBA20_004031"
                     /pseudo
     CDS             5034..5612
                     /locus_tag="QBA20_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1705 a         1623 c         1496 g         1561 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatatataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct
     4441 gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aatgacccaa
     4501 tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc
     4561 tggatactat cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaagctgtat
     4621 atattcagta ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct
     4681 cggcggtcaa acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca
     4741 gcaccgcgca aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca
     4801 ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc
     4861 gcatgagcgg tggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca
     4921 aaatgattgt tatcgatccg cgatataccg atacggctgc cggtcgtgaa gaggaatggc
     4981 tccctattcg cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta
     5041 acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa
     5101 ccttacccgc agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg
     5161 acgataaaac agcaaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc
     5221 gtatcatcaa actggcgcgt gaaattggca caacaaaacc cgcttatatc tgctagggct
     5281 gggggccgca acgccaggca aacggcgagc tgactgcacg cgctattgct atgttaccta
     5341 ttttgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata
     5401 ccattacgat agaacgccta ccggtgctgg ataatccggt caaaacgtca atctcctgct
     5461 tcagctggac ggatgctatc gatcatggcc cgcaaatgac ggcactccgc gacggtgtcc
     5521 ggggcaaaga taaactggat gtgcccatta agttcatctg gaacttcaag gacttgaagc
     5581 ggacccttag taggcctgag gtcctgcatt gagtcaagac cttgtgtcat tagacgggat
     5641 gtctagacgg ctggtcagcc cccccggtgg cctcggtctg gacttgaggt gggcctatgg
     5701 ggggaagtct gctggcttaa tgtattagat ggtcattcac atttttctgt cagaaaccat
     5761 cggtcccact gtgagtccaa acctaaggac ctagaagtcc acccacagcc ttagacagag
     5821 tgtcgaacac atccacaccg agtcgaccag agaggaccat gtagagaccc acagagagga
     5881 acacgagttt atagaccaga gtcaggaggg acttcaggtc tgcggaagtc atagacataa
     5941 agcccctatc tgttatgtta tgttatatta ttatattaac aacaggtcca gaccattggt
     6001 ccagaccata gttagttaat cacagtgata gcccaccacc tgaagtcctg ctgatattca
     6061 gtccagactt acctctgctc atggtcttac ctccctcggt ctcctgacct tgggtatcga
     6121 ctacagcttg ctgagcgtca tctggaggag ctacaggagg cgggaccgaa gccccacctt
     6181 gagtcccacc accacctgtc aggcccttgg cgatggacct gaagccttta ctaatgctct
     6241 tacccattgg tcttacgctt cggtcttggt gaacttcagg cgtttgacta caggcaggac
     6301 ggcagtagct gagccagtgc ccatcttgac ggtcgtagca gcttcagcga caacgaacac
     6361 tttaccgagg atttccaagt cgtgg
//
LOCUS       DAOFMX010000156         6377 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.158, whole genome shotgun sequence.
ACCESSION   DAOFMX010000156 DAOFMX010000000
VERSION     DAOFMX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.158"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(107..1816)
                     /locus_tag="QBA20_004032"
     CDS             complement(107..1816)
                     /locus_tag="QBA20_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HDQ6294367.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2248..2967)
                     /locus_tag="QBA20_004033"
     CDS             complement(2248..2967)
                     /locus_tag="QBA20_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HDQ6294368.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3092..3648)
                     /locus_tag="QBA20_004034"
                     /pseudo
     CDS             complement(3092..3648)
                     /locus_tag="QBA20_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3632..3890
                     /locus_tag="QBA20_004035"
                     /pseudo
     CDS             3632..3890
                     /locus_tag="QBA20_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3999..4577)
                     /locus_tag="QBA20_004036"
     CDS             complement(3999..4577)
                     /locus_tag="QBA20_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294369.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(4998..5235)
                     /locus_tag="QBA20_004037"
                     /pseudo
     CDS             complement(4998..5235)
                     /locus_tag="QBA20_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(5422..5851)
                     /locus_tag="QBA20_004038"
                     /pseudo
     CDS             complement(5422..5851)
                     /locus_tag="QBA20_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5899..6084)
                     /locus_tag="QBA20_004039"
     CDS             complement(5899..6084)
                     /locus_tag="QBA20_004039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294370.1"
                     /translation="MDAGNKKLVFWFVRVDDEGYPEIARCMEREFATIPAGINADGMY
                     CPECGTVHWPDGVIPPF"
     gene            6247..>6377
                     /locus_tag="QBA20_004040"
     CDS             6247..>6377
                     /locus_tag="QBA20_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDQ6294371.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCGMFVRFR"
BASE COUNT         1762 a         1460 c         1138 g         2017 t
ORIGIN      
        1 gttgccaatt gaggtggttg cctgaaagtt ggcagttacc cggtcactac accttgttgg
       61 agtgaacata tcaaaaacag gtaattacac aatttaaatt acagtctcat aaaaaatgat
      121 taaatctatt tattatttca ataaacgttt ttttcatctt ctttgaaaat tgttctgcta
      181 tagtatccgg ttccagattg ttctctttta ttattctaca atactctaat attgtttctt
      241 tgtctctatt tactacagat acaaatacac caggaatacc atctttatta gttgcggaca
      301 gcatttcgac caatcgtctt gtggtaaaac tatagttttt tagaatatta caaaaggcac
      361 gtattgtttc ggtatggcca ttataaaatg ccataaaaag tcctgatgta ccgttgggat
      421 tttttatcca taataattca tctatatatt cctggtgagg ggggagtatt tttagtatat
      481 ctccatacgc cataatggcc tttgtgtgcc cattttgaag tgccataaac aacccatgct
      541 ttactttcaa taattcttta atttgttcac tactaagcat tttcttatta agcagacttc
      601 cgaacatacg aatactatct gcatgtccat tctgcaatgc aaaaaataac ccaggtaaat
      661 catggctgtc tttcgcagct aatagatata atctcttatc ggaagttaac ggggttttaa
      721 agactatttc cccataggct tttattgtgt ctacatgtcc gttcataaaa gacatgcata
      781 aacccggcac attgtttgcc cctaccgcag caagcaatgt aagtattgtt tcttgactaa
      841 gattgcacat atttaatatc ttaccatata cttgaataat gtctgaatat ccgttttgaa
      901 gtgctatata aaatgcagga gtaccatcag agcttctcgt ggccagaagg ttcaccagtt
      961 tagattgaga aatttcacca tttttgtatt tttccatcag tataaataat acttctgtca
     1021 gattctttgt tatcgcatga tatataactc tggaattgat caattcatct ggtaaaagtt
     1081 tcactgtgtt tgttagaggg gaattatgcc tatcttcaaa tattacctgc ccgggttgct
     1141 ccagaccata aattttccaa taaaaatcat caaaaaaatc cttagcggat aattgactca
     1201 gttcgaaatg gctatcacaa gtaaatacag tacgtttatg agtaacagtt cgattaggat
     1261 cgaaaaactg aacaacccat ttttcacttt cttgtacgcg ctttattttc aaccctaacg
     1321 ccatttggtg gtttaatgtg catgctgata atgttctgac aaagtctcca ttttttttca
     1381 tgttataaaa aaaattagta ataactgttc cccacttttt attttccaca aaataaatat
     1441 aagctggtga ttttgaaaaa tcttccgttt tttccaaaca tacatttttt gcaatagcat
     1501 caggggtgct aagaagctga tagtttacct tcccatttgt ttccataaaa ttcatttccc
     1561 aataggaagc taggtgacgg caaactattt gtcttccata tatatctttt gccactccat
     1621 ttatatttac ctccacattt ctcttttctg aaaaataagg tgctgtttta ttcttaaact
     1681 gaattagttc actggagttt gagaccctca taatagaact tgttattgtt ctattatctc
     1741 tgttaacaga catatctgtt gataacgaat tttttgttga aaatatacta gatgaaaatg
     1801 ttttatttaa cggcatagaa gacaaccata gagatgttat aatactatta gattgcagtt
     1861 actaatagta aaagcctcct tacaaggagg tagcttattc agaatgctcc tataagatag
     1921 cgagctcgtc aacgatacag atgatctgat tatattgtgc aaaaggagaa caggaaaata
     1981 ggcactggaa aagatgaagg cctgatggga gcattaacta atgcaaaatc cgacaggtta
     2041 atatcccgaa aagcaatcaa acgactcaaa caggagatgc acgctgaaat ggtggacaac
     2101 tgttcggatt tcagatgagt agctcagcag tttatgagcc gaatgctctg tcacgggcag
     2161 gaatcaggct gcatacgcag tgccaaatct aatcgtagtt attctaacca gttttcaagc
     2221 tacaaggtgg tgtagctggc atcttctcta ctctatcatc aaacgataaa atggttcctc
     2281 tcgtaacatt tgctcttgcc tttcacttcc atctcgatcg cttcgtagtt cattacgata
     2341 gctgacatat ttccagtctt caggtctaac atctgacgcc ggatactccc cgggagcgat
     2401 tttgcttctt aatagattag actcgagaca cattataaat tgtcgtattt tatgaagtaa
     2461 taaagcacta tattgcgagc attcctgatc tgattttaca tatagcgtaa actgagctcc
     2521 tatccccaca cgagattgct gagaaactcg attcatatca gttatcttcc atttatctat
     2581 aggactgtcc tctgaaaata ataaaccaga caaaatttgg aatgccagag gaacttgctc
     2641 cctagctata ctaatatgaa acttgtcccc aacaaaatcc cccttactct gaggattttc
     2701 acgacatgca tgaataaaca catctccact atggttcaca atctggaatc cttggtacat
     2761 gacagaaaaa gattgacata tatcagagta agttggcacg ttcgatgcta ctttgaaata
     2821 gtacgctggg tattgccgta tttggttgta caatatattg aagttatttt tcaatctttc
     2881 attcgcagaa agcatctgcg gtatttcaga cctcacaaca ttcaaagaat ctaaatttag
     2941 ttttagacag ggctttttta tgggcataga aaacgtcctc tataaaatag atatattatc
     3001 ttatattgat tgtttatcat cttttataaa acgatacatt atatgtaatg tatgtaagcg
     3061 taagggtcct ctccgatcat ggtgggaagg cttagaacgc catattcagt ttcccatagt
     3121 ggaacatcac tcccagctta aagcgctccc ggttgcggta gcccttggct tttattctca
     3181 gcagcctgat cttgctgttc agtgcttccg catttccgtt tgatacgcca tggcgcatgg
     3241 cgttcaggat accgtacaac cttttcccta tcgttttagc cacattcttc atcatgggga
     3301 cgtcactgtt ggccgcagcc atctctgcca gtcgttgcgt ctttcctcgc tccatggtct
     3361 gctccagata tcctttgcca gttctttcag cgcccagcac aggctcgtca gcttcatctg
     3421 cgcacgcagc catatcagct tttcctgcct ggattcggtc atccatttat cgctgtgctg
     3481 ccacaggaag cgagttccct ttgcctgacg acgactttca acaggaagat gcggatgttc
     3541 attctggcgg gttttatcca ccacctcacc cggttgtttc gccacatgaa agcgatcgaa
     3601 ggctattttc tcaaccgcat tgggtaagtg gatgcgcgct gctcttagtt tgctgagcca
     3661 gtttagcatt gccttctaca tgaccgatgc ctgagccggt ataccgaact ttattgtcct
     3721 caaccagtca tgttataagc aagaaatgca ctcagcggat agagcgacat aatctgaacc
     3781 tgcggacaca tcttaagcgg cttacccgca aaaccatatg cttctcaaag tctgatgata
     3841 tgcactacaa gatcatcggc tggtatctga caatcaacca ccaccactaa atctgcgtca
     3901 cgacctgcaa ctgagtctgg ttgaacaggc tgaggccctg ctgaagcaac gcgaacctct
     3961 cgactcttgt tggattttac caaatagttt ttctaaaatt aaacacattt atataagttc
     4021 tttgctgaac aaaacgcagc ccgccatcct ttttcattca taaattcagc aatggcagat
     4081 aaaacttttt catcaagcat ataaaaaata atgccagtag actcaatatg cctcatacaa
     4141 ataaggccgc acatatccgt aatatatttg tcaatgctgc taccatttgc atggtaatat
     4201 gtcatgctat tatctgatga ttttacatat gcagtatgac atatttttcc ttcacgatta
     4261 acaaaactga caaaaccact ttcaccagga gtaatatata aagatgaatt taacgtgcgc
     4321 tcactaatat tcattaattg ttgaaattca atggaatagt tttcagttaa tgtatctatt
     4381 atcttggctt tgtctttctt tgttatctca ttgtcttttt cggcaagaaa aattgccact
     4441 ttatgacatg cattcagctc gttaatttta cctgataaaa ctgcttttct taccacatcg
     4501 ttgaactcat cgatagtgtg ttttttatta tatatatcat gaaaaatata gcctaaagac
     4561 ttaaacgaga ctttcatttt atattctcct taaatattaa caggatatat atatcacatt
     4621 ttcctctata ttttcagaaa ccagctatca ttttccccat tactggtacg tcgcttcaga
     4681 tatttccgca gcaagcgata accctgcgca ctatgaggat gccattcatt ataatgtttg
     4741 aacaccttcg cgagactact ttactgctgt taacacgtca ggttcgaaca taacactgat
     4801 ttaatcatat ttcatcgttt tgacgaagtt ttctgccatt ccattttgca ccgccgtgtg
     4861 tttgggggca aggccgacca tctgtgtttc attcgccaga tataagtgat gagatggcat
     4921 tctccctcct tcacggcaga agcattttga ccgtttaggg gatgcggacg attttcatgc
     4981 ctgctaagat actttcgtta ctgatgctgt ccgttttcca ccatttcatc atcactgatt
     5041 ggcgttgtgt ttttggtcag cactccagct ttacgtttct cttttaaccg gtaactctct
     5101 cctttgatat tcagcgtgta gcagccgatc taggatcgct gttgccagca cgtgatctcc
     5161 gaacatttct ccccagtctg cgaccccttt gtttgacgtc agtatgatgc tcgctttttc
     5221 atatcgacag ttcatcgata ggaatttaaa tccccaaaag attaaaaaag caccgaagaa
     5281 tgcgcgttta ttcaacacaa cctgatcatc aagtaatcgg tatagacact taatattgct
     5341 tacattgatt gattcaatgt atctctgatg gttttttgct ccgttcccct caacatggac
     5401 gcaacttccc ctctgcgact ctcagccgca ccaccgtatt cgagccagcg gctcatgcat
     5461 cataacctgc tctgccagac ggtaacccct cttcagcccc gtaaaccgca tctgacctcc
     5521 gcgcagctcg cacttcagcg ggtcaacctt cagtcactgc tgatacatcc ctctccttgt
     5581 gatttgcctc gccgtttttc tcactgtctc cgttatggcg tacaccacat cttccagtaa
     5641 tcgccgcttc accggactca ggaccccgta tcaccatccg gaacccctta tccgccacat
     5701 gccaggagaa cctttccata aactcgtcgc cactcatcac aaggttttct tctcgttttg
     5761 tccggtggct gttgtaacgc aggccaattt catcctgacc ggcataatgt tccaggcggc
     5821 tcatctgcac cggctgcttc tccagccagt taagccatcg gtttccctgc tgaaaaatat
     5881 cggcaaaacg gggaagcatc agaaaggcgg aataacgcca tccggccagt gaaccgtacc
     5941 gcattcaggg caatacatgc cgtcggcgtt gataccggca ggaattgtcg caaattcccg
     6001 ctccatgcag cgggcaattt ccggataccc ttcgtcatca acacgtacaa accagaagac
     6061 cagttttttg tttccggcat ccacaaagaa cggaatattc aggtctgcgc aacactcaac
     6121 ggcatcgtca aaattatcaa agcgcagaac ctcagcttct tcgtaaccgg ctcatttaaa
     6181 ccgtctggtc tgtttcctcc ggctccacaa aaataatgtc catcattttg aagtggacac
     6241 tatcgtatgg aataccggac ctggatcact gaagctttac gccttcattt cgaagaacat
     6301 ttaccacggg ttgtagccgg acgtcgcctg ggcgtaccaa aatcaactgt ctgtggtatg
     6361 ttcgtgcgct tccgcaa
//
LOCUS       DAOFMX010000157         6374 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.159, whole genome shotgun sequence.
ACCESSION   DAOFMX010000157 DAOFMX010000000
VERSION     DAOFMX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.159"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            58..1384
                     /gene="pgaC"
                     /locus_tag="QBA20_004041"
                     /pseudo
     CDS             58..1384
                     /gene="pgaC"
                     /locus_tag="QBA20_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1386..1799
                     /gene="pgaD"
                     /locus_tag="QBA20_004042"
     CDS             1386..1799
                     /gene="pgaD"
                     /locus_tag="QBA20_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDQ6294372.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="QBA20_004043"
     CDS             complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="QBA20_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDQ6294373.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="QBA20_004044"
     CDS             complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="QBA20_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDQ6294374.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4516..5643)
                     /gene="efeO"
                     /locus_tag="QBA20_004045"
     CDS             complement(4516..5643)
                     /gene="efeO"
                     /locus_tag="QBA20_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDQ6294375.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYTAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5701..>6374)
                     /locus_tag="QBA20_004046"
     CDS             complement(5701..>6374)
                     /locus_tag="QBA20_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDQ6294376.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1532 a         1680 c         1536 g         1626 t
ORIGIN      
        1 accaacctga aatagacctt attcgtcctg agttttcaac agcctggtat ccgaaaaatg
       61 attaatcgca tcgtatcgtt ttttatatta tgtctggtgt tatgcatacc cctatgcgta
      121 gcgtactttc actctggtga actgatgatg aggttcgttt tcttctggcc gttttttatg
      181 tccattatgt ggattgttgg cggcgtctat ttctgggtct atcgtgaacg ccactggccg
      241 tggggagaaa acgcaccagc tccccagttg aaagataatc cgtctatctc cattatcatt
      301 ccctgtttta atgaggagaa aaacgttgag gaaaccatac acgccgcttt agcacagcgt
      361 tatgagaaca ttgaagttat tgccgtaaat gacggttcaa cagataaaac ccgtgccatc
      421 ctggatcgca tggctgcaca aattccccat ttgcgggtca ttcatctggc gcaaaaacag
      481 gggaaagcca ttgcgcttaa aaccggagct gccgcggcga aaagtgaata tctggtgtgc
      541 attgatggcg atgcgttatt agaccgcgat gcggcggcat atattgtgga accgatgttg
      601 tacaacccgc gtgtgggtgc cgtaaccggt aatcctcgta ttcgaacacg ttctaccctg
      661 gtgggtaaaa ttcaggttgg cgagtattcc tcaattattg gtttgatcaa gcgaacccag
      721 cgtatctatg gaaacgtatt taccgtttcc ggtgttattg ccgcatttcg tcgcagcgcc
      781 ctggcagaag tgggttactg gagtgacgat atgatcaccg aagatattga tattagctgg
      841 aagctgcagt tgaatcagtg gacgattttt ttacgagcca cgggcgctgt gctggatatt
      901 aatgcctgaa acgttaaaag ggctgtggaa acagcgcctg cgctgggctc agggcggtgc
      961 agaagtattc ctcaaaaata tgacaaggtt gtggcgcaaa gaaaactttc gaatgtggcc
     1021 gctgtttttt gaatactgcc tgacgacaat atgggccttc acctgcctgg tcggtttcat
     1081 tatttacgca gtccaacttg ccggtgtacc gttaaatatt gaattgacac atatcgctgc
     1141 gacacatact gccggaatat tattgtgtac gttatgttta ctgcaattta ttgtcagcct
     1201 gatgatcgag aatcgctatg agcataatct gacttcatcg cttttctgga ttatttggtt
     1261 cccggttatt ttctggatgc tgagcctggc aacgacattg gtatcattta cacgagtcat
     1321 gttgatgcct aaaaagcaac gcgcccgttg ggtaagtcct gatcgcggga ttctgagagg
     1381 ttaatatgaa caatttaatt attacgaccc gacaatcacc agtacgttta ctggttgatt
     1441 atgttgccac aaccatcttg tggacattat ttgctttgtt catattctta ttcgccatgg
     1501 atctgctgac gggttattac tggcaaagcg aggccagaag ccgacttcag ttctattttt
     1561 tgctggcagt ggcgaatgcc gtcgtgttaa ttgtctgggc gctgtacaat aagctgcgtt
     1621 ttcaaaaaca gcagcatcat gcagcctacc aatatacgcc gcaagaatat gcagagagct
     1681 tagcaatacc tgatgagctc tatcagcaac tacaaaaaag ccacaggatg agcgtacact
     1741 tcaccagcca ggggcaaata aaaatggttg tttcagaaaa agcgctagtc cgggcataaa
     1801 cacccaaaac aaagcccggt tcgcccgggc tctgcaccga taatacactt aactgtatgc
     1861 atgcagcgta cgttggcaaa gtgccgaacg tacgcaatcc tcttttccga accggacgat
     1921 cccaaccatt tcatcttctt cgaaacgttc cagcgcgtca cttaatccgg agcacacgcc
     1981 gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata accgtcacgt tctccccgag
     2041 gcgggttaaa aacattttca tttgcgcggc agtcacattc tgcgcctcgt caagaatgac
     2101 aactgcattt tcaaaggtac gtccacgcat ataggcgaac ggcgcggttt ccaccttccc
     2161 tatttccggt cgcaggcagt actgcataaa ggaagcccct aagcgccgga ccagcaagtc
     2221 gtagaccggg cgaaaatagg gagcaaactt ttctgcgata tcaccaggta agaagccaag
     2281 atcttcatcg gcttgcagaa ctggacgggt gacgataatc ctgtcgacat ccttatgtat
     2341 cagggcctct gccgcttttg ctgcgctgat ccaggttttt ccgcacccgg cttcgcccgt
     2401 ggcgaatatc agctgcttac tctcaatagc ctgcagatag tgcaattgcg cttcatttcg
     2461 cgcaaggatg ggcgaggtat cgcgactgtc gcgggccata ccaatggctt ctacgccgct
     2521 catctgcaca agcgaggtaa ccgattcttc ttcacgctgc ttatggctgc gcgaatcccg
     2581 tctcagcaca cgttttgcct cgcgacgagc tttgatcact gctttttgtc ttcccatgga
     2641 gagcaccttg agttgtttgt attcatcaca cgcgccgttg gcagcgcgat tatgcgcacg
     2701 aacatcagag ggttggcttc cttgtaagcc atagtttgct tttggataaa atgccgaaaa
     2761 acggctacgc acaccgtttg cggcgtcggt aacacgagta aagaaaggaa gtgagaaaaa
     2821 tacagagtga cgaagttgct accggaggag aaatctccgc gagtggtgga gcgttgctta
     2881 acaaaacatt gtccagaaca ggacgttacc atccgcgatc tccatagtga ctgactatca
     2941 ctgccgggaa cttccgctgc tacttaataa gtacaacaga actcgcattc attgcaacaa
     3001 tatattcact tatatttaac gataaacacc catttcggtg acattaattt ctactggaaa
     3061 gatgacagag tgatgacagt gatggaaaaa gctgtgtgct ttccgcagga ttcgcagggc
     3121 tggtaaaatg tgcctcgtgt ttgccggatt cggcgcgaac gccttacccg acctacaagt
     3181 gattaataat ttgtgcacaa aactcatgcc cgataaacct gcgcatcggg catatttgcg
     3241 tcgttcaaac ctgcaatagc gcccttccca gataatcgtt cgcgtccttc actcccggca
     3301 gcgaaaaaaa ataaccgccg ccgataggtt taacgtattc ctccagcgct tcgccattga
     3361 gccttttttg tactgtcagg aagccttttt ccagatcgtg ttggtagcag acaaacagca
     3421 accccatatc cagttgtccg gagttggtga cccccaacga ataactgtag ccgcgacgca
     3481 gcatcaggct ggactcactc tccgccgtgc ggggattcgc cagccggata tggctgtcca
     3541 gcgcgatcac cttcccttcc gggtcgctgg cgtaatcagg cacatcatgc tcatgctgca
     3601 ttcccagcgg cgcaccggtt tgcttatcac ggccaaaaat cgtctgctgt tctttcagcg
     3661 gcgttctgtc ccaaaattcc actcgaaact gaatcaagcg tactgcctga tagctgccac
     3721 cgattgtcca cgcaggctcc tgctgatctg ccgttaccca caccactttt tgcatcaact
     3781 tatcattctg gctatcggga ttggcagtgc cgtctttgaa acccagcaaa ttaatcggcg
     3841 tctctttgcc tttactacgc gccgcgtgat cggaaataaa cccttcccgc ttccagcgca
     3901 cactgagcaa atccggcgtg tgtttgatga tatcgcgcag cgcatggttc accgtgtcct
     3961 gggtgttggc gcaaatctgt agcaacacat caccatgaca taacgccgca tccagcgagt
     4021 cgttggggaa acgcgtcatc ttctgcagct tttttggcat ctgtggcgca aggccaaagc
     4081 gctcatcaaa caatgagtga cccaccgata acgtgatggt gagattatcg ggcgcaatgt
     4141 agccgccaag aatgccggaa tcgagtggtg gcaggcgcgg atttggcgtt tctggtgctg
     4201 ctccgccctg actcagaaaa gcaaaacgct gagtcaacaa gcgaaacaac cgctcaagat
     4261 cggctttatc gctggcaagc acatcaaacg ccaccagcat cattgcggcc tgttgtggcg
     4321 tcaggatccc tgcctgatgc tcaccataaa acggctgttt ctcattgcgt gcatccggtg
     4381 aaagcgtgcc cggtgcactt tgcgtttttt gtgcatgagc gaccggacaa cttcccgcca
     4441 gcgcaccaat ccctttcagt aaacgtcggc gtgacggttc attcacgccg ttttcatctt
     4501 tatactgcat aacgcttaat ccagtcccag cacaccgcga agttgcgcca gatcttccgc
     4561 cagcgcagta atcggccctt tcagcgcatt ccggtcggca tcggtcaatt tgtcgtaggt
     4621 ttcaaaaccg tctttagtac ggtatttcgc cagaatggta tcgacctttt taaagttggc
     4681 atcgactttt gccaacagtt ccgggttggc tttttgcagt tgtggacgca gcagatcgac
     4741 aattttctgc gagccttcaa cgttagcctg gaaatcccac agatcggtgt ggctgtagcg
     4801 atcttcttca ccgctgattt tgctggctgc cacttcctca atcagtccgg ctgcgccgcc
     4861 gaccactttt gaaggtggga aagccagttc actgatgcgt ttttgcaaat cgaccacatc
     4921 ggtataaagc tgctcagcgt actgatccat ccctttggtg gtgttgtcgc caaacaatgc
     4981 tttttccaga cggtggaaac cagtgaattt tgggtcggcg gctttttgct cgtaatcatc
     5041 ttcacgggcg tcaatgctgc catccagatc ggagaacagt tcagcaatcg gttcaatacg
     5101 ctcatagtgc tggcgcgtcg gcgcatacag tgctttcgct ttttcgatat cgcctgcttt
     5161 aatcgcgtcg gtaaaggctt tggtgtcggt caccagctgc gtggtttccg ccatgacata
     5221 cgctgtatat gcagtaattg caccaccaag acttaacagc gcatcacttt gcgccgcatc
     5281 cgccgttgcc tcacctttga cgatcaactt ccctttcggg ttagtcagca gaccgcaggt
     5341 catatcgtat tcgccaggct gtaaattcgc cgtcattttc tggctaaaac caggggcgat
     5401 attttcccgc tcttccacca ccatcacgcc tttgaggatc tcccactcca gcgccttctg
     5461 gctatggttc tgaataatga actgtgtttt cccggcgtta accgtaatgg tcatcggttc
     5521 gcactgctta tccgtcacgg tcactttgac ctgcggcaca tcagcggcgt tagccataaa
     5581 agcagaagaa aacagcgcag ccacgctcaa ctgcaatgcg ttacggcgga agttaatggt
     5641 catgacgaga ctatcccttt aaagtatgtt gtaactaagc aagagtttgc gtcgtatttg
     5701 ttacgcggag cgagacgctg tcgcccctgc gcgtggtggc agagcaaatg ccaccagcgc
     5761 cgggatgaga taaataaacc agacggcgac ttcgctgacg ctcggtgctt cctgatagcc
     5821 aaaaatccct tccatcagcg tgccaaacag cgagtgagtt gagaggaccg cgctcatatc
     5881 gaaggcgatt tcctgaaagt ggttccacaa tccggcttca tgaaatgcgc gaatggcacc
     5941 agccgccagc cctgcggcga cgaagagaat aaacaggctg gtccatttaa aaaatgcacc
     6001 aagattgagg cgaataccgc cccagtagag caggaagccg agcaccacgg cagtagcaag
     6061 accgagcatt gcacccagcg gcggccagat cccgacatct tgttgaaatg ccgccagcag
     6121 gaaaaagacc gactccagcc cttcccttgc aacggcaaaa aagaccatca tcaccagcgc
     6181 ccagccatga tgatttccac gctgcaatgc gctatcgact gcctgttcca gttgcacttt
     6241 gacgttgcgc gacactttgc gcatccagaa aaccatccag gtaaggatca ccacggcgat
     6301 caccgccacg ataccttcaa acagttcctg ctctttttgc ggaaattcgc cggtggtttc
     6361 gttaatgaag atcc
//
LOCUS       DAOFMX010000158         6321 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.160, whole genome shotgun sequence.
ACCESSION   DAOFMX010000158 DAOFMX010000000
VERSION     DAOFMX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.160"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            73..378
                     /locus_tag="QBA20_004047"
     CDS             73..378
                     /locus_tag="QBA20_004047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6294377.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="QBA20_004048"
     CDS             complement(454..918)
                     /locus_tag="QBA20_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDQ6294378.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="QBA20_004049"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="QBA20_004049"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDQ6294379.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="QBA20_004050"
     CDS             complement(1787..2476)
                     /locus_tag="QBA20_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDQ6294380.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="QBA20_004051"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="QBA20_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDQ6294381.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="QBA20_004052"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="QBA20_004052"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDQ6294382.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="QBA20_004053"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="QBA20_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDQ6294383.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1609 a         1709 c         1565 g         1438 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc t
//
LOCUS       DAOFMX010000159         6310 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.161, whole genome shotgun sequence.
ACCESSION   DAOFMX010000159 DAOFMX010000000
VERSION     DAOFMX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.161"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            103..282
                     /locus_tag="QBA20_004054"
                     /pseudo
     CDS             103..282
                     /locus_tag="QBA20_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            808..1986
                     /gene="ltrA"
                     /locus_tag="QBA20_004055"
     CDS             808..1986
                     /gene="ltrA"
                     /locus_tag="QBA20_004055"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDQ6294384.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2069..2248
                     /locus_tag="QBA20_004056"
                     /pseudo
     CDS             2069..2248
                     /locus_tag="QBA20_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2278..2760)
                     /locus_tag="QBA20_004057"
                     /pseudo
     CDS             complement(2278..2760)
                     /locus_tag="QBA20_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3016..3996)
                     /locus_tag="QBA20_004058"
     CDS             complement(3016..3996)
                     /locus_tag="QBA20_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDQ6294385.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3996..5195)
                     /locus_tag="QBA20_004059"
     CDS             complement(3996..5195)
                     /locus_tag="QBA20_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6294386.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5517..5855)
                     /locus_tag="QBA20_004060"
                     /pseudo
     CDS             complement(5517..5855)
                     /locus_tag="QBA20_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5922..>6310)
                     /gene="istB"
                     /locus_tag="QBA20_004061"
     CDS             complement(5922..>6310)
                     /gene="istB"
                     /locus_tag="QBA20_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HDQ6294387.1"
                     /translation="KVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGY
                     LPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSH
                     VVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1710 a         1454 c         1509 g         1637 t
ORIGIN      
        1 actcctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
       61 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcagt tgctgaactg
      121 ctggaaccgc tgtatgacat cctgcgccag tatgtgctga tgcccggtaa agtccatgcc
      181 gatgatatcc ccgttccggt ccaggagccg ggcagcggta aaacaaggac tgcccggctg
      241 tgggtctacg ttcgtgatga ccgtaacgcc ggttcggaaa ttgcagcata agttatccat
      301 gctctgaggt gggtggaggt gggccctccg gtcagggttt acaggagcac agaccaaacg
      361 acccggtgct gcttcattct aaggaatgag gtggtgagcg actcgggaaa aggcagagat
      421 ctactgtcac gtggggttcg ggaagctgaa cgaatgtaaa ctgtccgatg aagcatcgaa
      481 atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc agaagacacc
      541 tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc agaccatctc
      601 agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac ccattcgtag
      661 tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca ttcattgcag
      721 aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt gaaataccaa
      781 aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc ccccggatgc
      841 gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata caagatctgg
      901 aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg gatcccgaag
      961 tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat tgctcaggga
     1021 gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga ttcatatggt
     1081 taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg gtgctggcgt
     1141 tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag acatgatctg
     1201 gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta ctgtcgacgc
     1261 tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg aacacgaggc
     1321 actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca ctatgcattt
     1381 gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc tgacgatatt
     1441 gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt atcggagcgc
     1501 ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta cattgacacg
     1561 tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt caaagtgcgt
     1621 acgctgaaga atttcaaagg cgaacggtac cgaaaatgca tgccgggtgc gtcaaatgca
     1681 gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc aacagctgag
     1741 agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat cgagtactac
     1801 ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca gtcacgtctg
     1861 ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg gaagctgacg
     1921 ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg tgcatcaaat
     1981 gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag cagccgggga
     2041 gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg aacatgcggc
     2101 ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag aaaaatggct
     2161 gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg atctgttgcc
     2221 ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag ccgcttactt
     2281 ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa gaagtccacg
     2341 aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat tcaaaaaagt
     2401 ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc catgttggca
     2461 ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct gataagcttt
     2521 aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt cccgggccca
     2581 gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt gcaccagcca
     2641 gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat gagcattgat
     2701 actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct gttcagacat
     2761 ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg aaggctgccg
     2821 tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga aactatcttt
     2881 ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa
     2941 acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggtg agttgctggc
     3001 gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag caatcatacg
     3061 atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc
     3121 ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca aagttacaac
     3181 cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta taagccgcat
     3241 aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa gctcaatact
     3301 cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca gtcgttcact
     3361 taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag gaaaaagaga
     3421 cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag ctgccgacaa
     3481 tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac gtactaaacg
     3541 caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg ccagatgctg
     3601 agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa ccttgcatag
     3661 caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc cagtccgaat
     3721 cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc
     3781 cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag tttgagtatc
     3841 cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag tattgagggt
     3901 aaatacatga actggagccg atatttcttc agtattattc aggatatttg tattcagcgt
     3961 acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct tataaattca
     4021 atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt tttcaaagct
     4081 tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa cgactcgccg
     4141 cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc
     4201 tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt cgacatgaag
     4261 ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg tacaagttca
     4321 ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt
     4381 gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag atgcgggcct
     4441 gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt ctcacgcaat
     4501 atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca gtttgatgca
     4561 ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac tgacggaacg
     4621 ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg cggtgcggtt
     4681 gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga tgactgggga
     4741 tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg agcacgcagg
     4801 gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag actaccgatg
     4861 aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg atgtgcatag
     4921 atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc tgggcgtttt
     4981 tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct tgcgcgcgtt
     5041 aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac atcagcctga
     5101 aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt atctgcacgc
     5161 tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta tgtaggattt
     5221 taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca tattgtacat
     5281 gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa tattactgta
     5341 agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc agttcaccac
     5401 cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt
     5461 ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag
     5521 tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc cacggaacgc
     5581 gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt ccggttgtta
     5641 cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg cacttctctg
     5701 gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt caacagggct
     5761 aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa cacaccgcca
     5821 gttttagtgt tgtacgcata ccagagccag tgttgtgtca acgacggatg aaaagtgatc
     5881 cacttatatc tccaccaacg gcccaatatt gatccaccgt tttactcagg attagcttct
     5941 gctataaccc cggcctttcg tttctgtctg agtcgatagc tttctccttt gatttgaacg
     6001 acatgtgagt ggtgtaagat acggcccagc atcgctgagg tcagtgctgc atcaccggcg
     6061 aacgtttgat cccactgccc gaacggtaga ttggatgtca ggatcattgc gctcttttcg
     6121 taacgtttag cgatgacctg gaagaacagt tttgcttctt cctgactgaa cggcagatag
     6181 cctatttcat caatgatgag caggcggggg gccattactc cacgctgaag cgtcgtttta
     6241 taacggccct gacgttgtgc cgtagataac tgaagtaaca gatctgctgc tgttgtgaag
     6301 cgaactttga
//
LOCUS       DAOFMX010000160         6196 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.162, whole genome shotgun sequence.
ACCESSION   DAOFMX010000160 DAOFMX010000000
VERSION     DAOFMX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.162"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..595)
                     /locus_tag="QBA20_004062"
     CDS             complement(<1..595)
                     /locus_tag="QBA20_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HDQ6294388.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLLWRHFRQHLAVRWRKVCGGWFLLIAISTLTTWQHHF
                     IDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIKIALP"
     gene            complement(585..2341)
                     /locus_tag="QBA20_004063"
                     /pseudo
     CDS             complement(585..2341)
                     /locus_tag="QBA20_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
     gene            complement(2357..3241)
                     /locus_tag="QBA20_004064"
     CDS             complement(2357..3241)
                     /locus_tag="QBA20_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDQ6294389.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3252..3857)
                     /locus_tag="QBA20_004065"
     CDS             complement(3252..3857)
                     /locus_tag="QBA20_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDQ6294390.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALYGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(4028..>6196)
                     /locus_tag="QBA20_004066"
     CDS             complement(4028..>6196)
                     /locus_tag="QBA20_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HDQ6294391.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1806 a         1557 c         1298 g         1535 t
ORIGIN      
        1 atggcagtgc tattttgatt cgacgttgat caagtttctg ataattccaa cgacggtcga
       61 cgggcaccat ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat
      121 gatgctgcca tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccacagactt
      181 tacgccacct cacagccaga tgctgacgaa agtgacgcca aagtagccat cctgtcaccc
      241 cactcacttc aggacggata aaactaaact tcagcgggta gagaaaaaaa ccgcagcagg
      301 ccattaccgt tgccagaata agccggtgga caagtcggcg ttgttcgaat gtcgagctac
      361 aaacgaacag cgaaaatcca tataaaagat ccagactcca gtaaggaaca atagtccagg
      421 gaagaaaagg gatcgccgtt tcccaaccga atacctgact ggggatatca tggctgttaa
      481 ggtcctgaac cgcggtgaac tgattaagag atccataggt gaagaagaaa aacggggcca
      541 gcaacaataa ccagccagcg ccttgtagca atacgttccg acgttcagtt atcacgacgc
      601 accgccatcg aaaccgtaaa aatgccccac acatcaatcc gttgagtgca tttatcaaat
      661 ccggcattac gcacgagcga atccatctcc ccttgcgaac gcacgcgcat tacccacggt
      721 ttaccatctt tatgactggt taacaccccg gcaatcagct ccagttgtgg gtgccacggc
      781 tgcccggtgt agatgagaat gccacccggt tcgatggcat tggctagacc tgcgagtgag
      841 tttttacctg ctcattttcg ggaaaaagct catataggcc agagacaatc gccagcgtag
      901 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
      961 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     1021 tgtaatcacg taacaaaata tcgcttacgg caggctcgtt tgccagcgca tccagtacat
     1081 agcgtccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg gcgtggagat
     1141 cggcaacggc ttgtttaatc aatatttgca aatgggtttt gcgctggcga ataccgcgcc
     1201 atccaacact gttcaggtag attttgtcga ttaaacgccc gaatgcgtta ctgccttgcg
     1261 gttgattttg atagacataa tccagggaac tgcctgaatc aaagccggta ctcattccga
     1321 gatgcaggcc cgcagagtgc gcaccgaaca atttcctcgc ctttcgcata aagcgatacg
     1381 ctaaatcaac cggcgataat ggcacgggtc caccagaaag gagccgccat cgatccgctg
     1441 atggtcctgt gcggtcttca tgctgataat caaatttttg cgatttgtta gcatataagc
     1501 ggcagataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaatgtgt
     1561 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     1621 aatcaatctg cggttggcga tgcaccacat agtcatcacc tgatatcaga agttgcgtgg
     1681 ggagcgtaat cgccgccgca tcgctaacaa tacgttcaga cgttttatag agatcgagca
     1741 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     1801 gccgatcgtg ggtcaaatag cgccctttca cataggaatt aataaaaaat agaccacgca
     1861 gacgatgcca taacgccagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     1921 ccggagaagc cagcaccagc ccacgaattg caggtgcata atcatgaacc catgtggcaa
     1981 caagcactgc gccgacgctt tgcgcgatca ctaccacctc ttccagtccg acctggctgt
     2041 cgctgacagc aaaacggaca aattcatcga catcctgcac tgagcgcata agagacgggc
     2101 tataaccacg cggccccgaa ttttttccat gcccccgggc atcccaggca taaaaagcag
     2161 tatcaggcat cgccagttca tcaacgagat gttgtagacg accagaatgt tcatgcccgc
     2221 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agtgggccag tgccgataat
     2281 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agggatgcgt gaattttcca
     2341 ttttctctat tttccttcag taacagcagt agcgaataaa ataaaaaaat accggagcgg
     2401 tgaaaattaa tgagtcaatc ctgtcaagga gtccgccatg tcctagtaat agttttccac
     2461 tatctttaac accaatatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     2521 taataccaat taacaaaccc gctaataatg cctgtaatgt atttagcggt gtcagcaacg
     2581 gtccaataat cagtgacgca atcatggtgg tgattacgcc ccccagcaga ccttccagcg
     2641 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     2701 actgtgcaat atcgtttgac tcggttaacg ccaaaagaaa taacaccagt aacgccccgc
     2761 cctggatatt tatggttggc aacattaata gccaggcggc atgactcaag gcaaaaacgg
     2821 tcattatcca tccccaaaaa atggcgctca cggtatgctg aaaaccggag gggtcaccca
     2881 caaaaactcg ccaggtggct aatatcagaa acccagtcaa aggtataaat aacagaaaca
     2941 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccaac
     3001 gcgaaaaatg tacggatata agcgtacaat attctttcaa tgccagaaaa cttatcagcg
     3061 taaaaaacgt caacgtcatc cagcgtgggc ctgaaatcac cagtgaaaaa caaatgacga
     3121 tgccccacca ggtacgaata cgtaatgtaa cttcatcccc ttttctctca ggtaaccgcc
     3181 atagcagaaa acggtttatc agcttgctaa aattaacaac gcgaaaagtg ttgccagaga
     3241 tttttccagc attatctttc agcgctcata agaagcacac tccgacaacg attaatcgca
     3301 gtacagataa gcagaattga agcaatgctc cataaaagat tattccattg catccactgt
     3361 ggataaatgg caacggccag accccacaga ccaaatatta acgcgcgatc acttttgcca
     3421 aatggaccgg cataactgcg aacaccgtta atcgtctgcg cgagtaaacc gcaaaactcg
     3481 gtcaatatgg tgcaaaataa catgagtatc acgagtgatg cgttactttc cggtaaaaat
     3541 aaaaacggta aatagagcgc aatatcggaa ataacatcgc cagtctcatt caaaatcgcc
     3601 cccagacgtg tttgctgatt gcactcacgc gccaacatgc catacagcgc attgagcgcc
     3661 atacggataa aaagcacgat gggcaatagc aaaaagagga tgggttgtgc caccaacacc
     3721 aacagcaatc cggtaaaaag agaaagcgcc agtgccgtaa gagtgatgtg attcgctgta
     3781 acgtggtgct tataaagcca aaacatcgtc ggccttaaca gcgactgaaa gagcggtttt
     3841 atctggtaga gcgtcattat caatccttga taattcggtt tgcgctatgg tgcatcaatt
     3901 cccctgacgg agatatcgca acacgccgct tcattaaaaa ttcagaatat atcaaggtat
     3961 tgggggctca ctgtcaggaa tgattatccc caataaatca agtagttaaa aattattacg
     4021 ctgttattca atacaactca tcaagtttcc tcatcgcttg ttcccgtatt gcgcgcgtct
     4081 taccgttaag cgccagctgg cggactaaat ccagttcagg gttaacaagc tctaacgcca
     4141 gcaaaggatc acgtttcagc ctcgccttca atacatcaac tggaaagcgg ggattattca
     4201 tcgccacaat ccaccactcg ggatcaattt ctgccgccag tgccgcagag ggggtgtggc
     4261 ggttgctaag tgcgccgaac ttgaaaaatg tgagattgct tttcgccagt gtactccacc
     4321 atatacgatc cggttgtttt gccagtacat ccagttgtgc taacgcatcg gggtcgagtg
     4381 cataataaac acgtaatgct tcatcaacct gcgcacggga gctattttcc tgcctcagaa
     4441 aaaacttcgc ccaaatatca ttgcgtggcg tgttttgctg taattccttg agaacaacag
     4501 cgggtaaatt atcccaacta actaatgcac tctctaaaaa gtcccagtct tccaaacgta
     4561 tcagggcggc ggcatttaat agcatctggt catcaacttc gccgtggcta atcagctcag
     4621 acaaaatcat atcagcgctg gtttcatatg catctgaatc tgctttcgga ttatttttcc
     4681 gatgcgataa agcacgttgc caaatttttt gtttcatcga cgtgctcaga tactgatttt
     4741 tcgccagatt actccacccc tcaggatcgt tgaaacgtgt caataaccgt tgtaaagcag
     4801 cgtcagagtg aattacctgc atatcccgac agcaaaagta ggcaggggat tttttctcca
     4861 tccttttaac aaaattaaac tgtaagttat ctggcaatgc taaaaataac acacgtatta
     4921 gatcatcgtc attctcgaat ttttggtata aataggctgc aactttgtca ctctcttgtg
     4981 gcgtcatccg caataattga ccatggtgtt tcatctctgc cagcttgaac gctaagcttg
     5041 tgacaacatt gtttgatgaa tcgttcgcca actgcattgc ctgattgtaa gtgagttgtc
     5101 ctccagcacc gacctgacga atagaggaat catcactgcg gatatagcca tcaataatgg
     5161 attcgggcaa taaccacgca tctttataaa gctcctgaat gatcatatcc caatctcttt
     5221 tcgccagagt catgacgata tccagaggga gattttcacg tagcgcaaga ttcaacggac
     5281 cgacaccatc aggtagacga ctcattcgcg tcattatttc gacagagagc ggttcacggt
     5341 cgagcaaccc cagaacaaca ggctggtaac ccaattgctg atcggtcaac tccagaatct
     5401 cattgtcagg catatatctc gccatacttt caatcgtttg ttcatcattg ctattaataa
     5461 gttgtttgcg taggttagct gaaattcctt taagccttgg gagagttcgc attatggcaa
     5521 gatcaaatgt cgcaatatct cgtccttcaa atgcagcaac gttgccttca tccagcattt
     5581 gcaattcctg cactaactca ggagaaggct tagggcgaaa cagcgcttct tttttcatct
     5641 gattacagac attccctgcc atgcgaatat tgcttcgctt aaaaatttct ttcagagcat
     5701 cttcatcatt tattagaccc caataatatg catcaggatt tattatcatc agcgcttttt
     5761 tgacattttc agtgtcattt ttgtcactcg cacggaccat gaattcacta tttgtcgaag
     5821 acgcaacaac aggtgcaatt atgtcattga taaatacccc acgcgcatta atactcccct
     5881 taatcgtaga cggattatcg taatatgagt cgataaatac cagtccactt gcatttatcg
     5941 aaccggtaat taacagtggt gaatccgtaa ctatattcgt tgccgtcacg ttaccgagaa
     6001 taacccatgg attagaaaga gagaaagtgg aaatattacc tttgactgtc aaattgccaa
     6061 gaacaatcaa tttttgatta ctctctatat taccttctac cactaaatcg ccacgatgca
     6121 ctttccagtc cgatgcagat aaaccgaagg ggacaagatt cacgctactg ttgatagctt
     6181 gttcaacttg ttgttt
//
LOCUS       DAOFMX010000161         6114 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.163, whole genome shotgun sequence.
ACCESSION   DAOFMX010000161 DAOFMX010000000
VERSION     DAOFMX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.163"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            437..1465
                     /gene="torT"
                     /locus_tag="QBA20_004067"
     CDS             437..1465
                     /gene="torT"
                     /locus_tag="QBA20_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDQ6294392.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1438..2130)
                     /gene="torR"
                     /locus_tag="QBA20_004068"
     CDS             complement(1438..2130)
                     /gene="torR"
                     /locus_tag="QBA20_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDQ6294393.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2217..3389
                     /gene="torC"
                     /locus_tag="QBA20_004069"
     CDS             2217..3389
                     /gene="torC"
                     /locus_tag="QBA20_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDQ6294394.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3389..5929
                     /gene="torA"
                     /locus_tag="QBA20_004070"
     CDS             3389..5929
                     /gene="torA"
                     /locus_tag="QBA20_004070"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDQ6294395.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5926..>6114
                     /gene="torD"
                     /locus_tag="QBA20_004071"
     CDS             5926..>6114
                     /gene="torD"
                     /locus_tag="QBA20_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDQ6294396.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         1491 a         1527 c         1689 g         1407 t
ORIGIN      
        1 gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat taccgtccat
       61 ttcggtccca gttaggacgc tttctcatca agccagtccg cccaccactg catcatctcc
      121 ctacgcttat cgagatactg agcatggttg taaattcaat taacccagtg cgttgacaaa
      181 aaattagcgc aagaagacaa aaatcacctt gcgctaatgc tctgttacag gtcactaata
      241 ccatctaagt ggttgattca tagtgactgg atatgttgtg ttttgtcgcg tttttcagtc
      301 tattatttaa tcattaagta atctaatgct atgattttta aattatttat gtgattttgt
      361 catcctttag gtgaaaaagg ttgagtcgca aagtggaatg catctagcat aaagccttat
      421 tattgatgag gctatcatgc gcgtactgct atttttactt ctttcccttt tcatgttgtc
      481 ggcattttcg gctgataacc tgttgcgctg gcatgatgcg cagcatttca cggtgcaagc
      541 ctctatgccg cttaaagcca aacgcgtatg gaaactgtgc gcgctttatc ccagcctgaa
      601 agattcatac tggttatcgt tgaactatgg tatgcaggag gctgctcgcc gctacggtgt
      661 ggatttaaaa gtgctggagg caggcggcta cagccagttg gctacccagc aagcacaaat
      721 cgaccagtgt aaacagtggg gcgcagaggc cattttgctc ggtagtagca cgacgtcatt
      781 tcccgacctg caaaagcagg tagcaaatct gccggtgatc gaactggtaa atgctcttga
      841 tgatccccag gtgaaaagcc gcgttggtgt accctggttt cagatgggct atcaacctgg
      901 gcgatatttg gtgcaatggg cgcacggtaa accactgaac gtgctgctga tgcccggacc
      961 cgataacgcc gggggcagta aggagatggt cgagggtttt cgcgcagcca ttgccggaag
     1021 cccggtgcgt attgttgata ttgcgcttgg tgataacgat attgaaatcc agcgcaacct
     1081 gttgcaggag atgctggaac gccacccaga aatcgacgtc gttgccggaa cggccattgc
     1141 ggcagaggcg gcaatgggtg aagggcgtaa cctgaaaacg ccgcttaccg tggtgtcgtt
     1201 ttatctttca catcaggtgt atcgcgggct gaagcgggga agagtgatta tggctgccag
     1261 cgatcaaatg gtctggcagg gggaactggc agttgagcag gccatcaggc aattacaggg
     1321 gcaatcggtg tctgataatg tcagcccacc gattttagtt ctgacgccga aaaatgccga
     1381 ccgagaacat atccgccgct cgctgtcacc ggggggattt cgtccggtct atttttatca
     1441 gcacacatca gcggctaaga aataaccttc accatgttgc gtcaccagta aatccgcgct
     1501 gagtttatga cgtaaacgac gaattaacac atcgacggtg cgcaggtcag ggttttccac
     1561 ccgacgcgca gaaagcatac gcagcagacg ctcacggctg agaatttcgc ccggattagt
     1621 cacaaatgcc accaacattt catactctgc gcgggtcagt ttaatcggct cgccatcccg
     1681 ctccagcgta tggcgcgaca cattcaggca ataacccgca aagcgatagc agtagtcctg
     1741 agtgtgcggt tgagcttgtc gcgcgaggtc gattcgccag agcagatttt tcacccgtac
     1801 taccagttcg cgcagttcca gcggtttggt gacgtaatcg tctgcgccca tttccagccc
     1861 aacaatacgg tcaatccgat cgctgcgtcc ggtaaccaga ataatcccca ccgttgagcg
     1921 ttctcgcagg gcgcgggtta acatcaggcc attttcatcg ggtaagttga tatccagcag
     1981 aattaaatct accggctgat tctgcataat ttcccgtagc ccggcaccgc tcgccgtaac
     2041 ggaaacggta tacccctcct gagtgaagta ggattgtaat cgcgcctggg taaccggctt
     2101 atcttcaaca ataacaatgt gatgtggcat cagagggttt tactcattct gttcatatct
     2161 gttcatattt gctcattaag atcgcttcac taaaccataa ttctacaggg gttattatgc
     2221 ggaaactctg gaacgcgcta cgccgaccca gtgctcgttg gtcggtactg gcgctggtcg
     2281 caattgggat tgtgattggc attgcgctga ttgtattgcc acacgttggg atcaaagtca
     2341 ccagcacaac cgaattttgt gtcagttgcc acagtatgca accggtgtat gaagaatata
     2401 aacagtcggt gcatttccag aacgcctccg gcgtgcgagc tgaatgccat gactgtcata
     2461 tcccgccgga tattccaggc atggtgaagc gcaaactgga agcgagcaac gacatttacc
     2521 agacctttat tgcccactcc attgatacac ctgaaaaatt cgaagccaaa cgcgcggaac
     2581 ttgccgagcg tgaatgggcg cgaatgaaag aaaacaactc ggcaacctgt cgctcctgcc
     2641 ataactacga tgcgatggat catgccaggc agcatcctga agcggcacgt cagatgaagg
     2701 tggcagcgaa agataatcaa tcctgcatcg actgtcataa aggtattgcc caccagttac
     2761 cggatatgag tagcggcttc cgtaagcagt tcgatgagct gcgcgccagt gctaatgaca
     2821 gtggtgacac gctgtactct attgatatta agccaattta tgcggcgaaa ggcgataaag
     2881 aagcctctgg ttctctgctg cctgcttcgg aagtgaaagt ccttaaacgt gacggcgact
     2941 ggctgcaaat tgaaattacc ggctggacgg aaagcgccgg acgtcagcgt gtactcaccc
     3001 aattcccagg taaacgcatc tttgttgcct cgattcgtgg tgatgtgcag cagcaggtga
     3061 aaacgctgga gaaaaccacc gttgccgaca ccaataccga gtggagcaag ttgcaggcca
     3121 ccgcgtggat gaagaaaggc gacatggtga acgatatcaa accgatctgg gcctatgcgg
     3181 attcgttgta caacggcacc tgtaaccagt gccacggcgc accggaaatc gcccactttg
     3241 acgctaacgg ttggatcggc acgctcaacg gcatgattgg ctttaccagt ctcgataaac
     3301 gtgaagaacg cactttgttg aaatatctgc aaatgaatgc gtctgacacc gcaggtaagg
     3361 ctcacggcga taagaaggaa gaaaaataat gaacaataac gatctctttc aggcatcacg
     3421 tcggcgtttt ctggcacaac tcggcggctt aaccgtcgcc gggatgctgg ggccgtcatt
     3481 gttaacgccg cgccgtgcaa ctgcggcgca agcggcgact gaggctgtca tctcgaaaga
     3541 gggcattctt accgggtcgc actggggggc tatccgcgcg atggtgaagg atggtcgctt
     3601 tgtggcggca aaaccgttcg aactggataa atatccgtcg aaaatgatcg ccggattgcc
     3661 ggatcacgta cacaacgcgg cacgtattcg ttatccgatg gtacgcgtgg actggctgcg
     3721 taagcgccat ctgagcgaca cctcccagcg cggtgataac cgttttgtgc gcgtgagctg
     3781 ggatgaagcc ctcgacatgt tctatgaaga actggaacgc gtacagaaaa ctcacgggcc
     3841 gagtgccttg ctgaccgcca gtggttggca atcgacgggg atgttccata acgcttcggg
     3901 gatgctggcg aaagctattg ccttgcatgg taatagcgtt ggtacgggcg gagattactc
     3961 taccggtgct gcgcaggtga tcctgccgcg cgtagtcggt tcaatggaag tgtatgaaca
     4021 gcaaacctcc tggccgctgg tattgcagaa cagcaaaacc attgtgctgt ggggctccga
     4081 tttgctgaaa aaccagcaag cgaactggtg gtgcccggat cacgatgttt atgaatatta
     4141 cgagcagcta aaagcgaaag tcgccgccgg tgaagttgag gtcatcagca tcgatccggt
     4201 tgtcacatcc acccatgagt atctggggcg cgagcatgtg aagcacattg cggttaaccc
     4261 gcaaactgac gtgccgctgc aactggcgct ggcacatacg ctgtacagtg aaaacctgta
     4321 cgacaaaaac ttcctcgcta actactgtgt gggttttgag cagttcctgc cgtatctgct
     4381 gggtgagaaa gacggtcagc cgaaagatgc cgcatgggct gaaaaactga ccggcattga
     4441 tgccgaaacc attcgtgggc tggcgcggca gatggcggcg aacagaacgc aaattattgc
     4501 tggctggtgc gtgcagcgta tgcagcacgg tgaacagtgg gcgtggatga ttgtggtttt
     4561 ggcggcgatg ctggggcaaa ttggcctgcc aggtggtggt tttggctggc actacaacgg
     4621 cgcaggcacg ccggggcgta aaggcgttat tctgagtggt ttctccggct ctacgtcgat
     4681 tccgcctgtt cacgacaaca gtgactacaa aggctacagc agcactattc cgattgcccg
     4741 ttttatcgat gcgatcctcg aaccggggaa agtgatcaac tggaacggta aatcggtaaa
     4801 actgccgccg ctgaaaatgt gtatttttgc cggaactaac ccattccatc gccatcagca
     4861 gatcaaccgc attattgaag gctggcgcaa gctggaaacg gttatcgcca tagataacca
     4921 gtggacctca acctgccgct ttgccgatat cgtgctgcct gcgaccacgc agtttgagcg
     4981 taacgatctc gaccagtacg gcaaccactc caaccgtggc attatcgcca tgaaacaggt
     5041 ggtgccgccg cagttcgagg cgcgcaacga ctttgatatt ttccgcgagc tgtgccgtcg
     5101 ctttaatcgt gaagaagcct ttaccgaagg gctggacgaa atgggctggc tgaaacgcat
     5161 ctggcaggaa ggtgtacaac aaggcaaagg acgcggcgtt catctgccag cgtttgatga
     5221 cttctggaat aacaaagagt acgtcgagtt tgaccatccg cagatgtttg ttcgccacca
     5281 ggcattccgc gaagatccgg atctcgaacc gctgggcacg ccgagtggcc tgattgagat
     5341 ctactcgaaa accatcgccg atatgaacta cgacgattgt caggggcatc cgatgtggtt
     5401 tgagaaaatc gaacgctccc acggtgggcc cggctcgcag aagtatccgt tgcatctgca
     5461 atctgtgcat ccggatttcc gacttcactc gcagctatgt gagtcggaaa cgctgcgtca
     5521 gcaatatacg gtagcgggta aagagccagt gttcattaac ccgcaggatg ccagcgcgcg
     5581 cggtattcgt aacggtgatg tggtacgcgt ctttaacgct cgcggtcagg tgttggcagg
     5641 agcagtagtt tctgaccgct atgcacccgg cgtggcgcga attcacgaag gggcatggta
     5701 cgatccagat aaaggcggcg agcctggtgc gctgtgcaaa tacggtaacc ccaacgtgct
     5761 gaccatcgac atcgggactt cgcagctggc gcaggcgacc agtgcgcaca ctacgctggt
     5821 ggaaattgag aagtacaatg gaacagtgga gcaggtgacg gcgtttaacg gccccgtgga
     5881 gatggtggcg cagtgcgaat atgttcccgc gtcgcaggtg aaatcatgac cacgctgaca
     5941 gcacaacaga ttgcctgtgt ttacgcctgg ctggcgcagt tgttctcccg tgaactggac
     6001 gatgaacaac tgacgcaaat cgccagtgcg cagatggctg aatggttttc gttgctgaaa
     6061 agcgaaccgc cgctcactgc ggcggtgaat gatctggaaa acagtattgc cacg
//
LOCUS       DAOFMX010000162         6055 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.164, whole genome shotgun sequence.
ACCESSION   DAOFMX010000162 DAOFMX010000000
VERSION     DAOFMX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.164"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            433..1341
                     /locus_tag="QBA20_004072"
     CDS             433..1341
                     /locus_tag="QBA20_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HDQ6294397.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1387..3378)
                     /gene="tkt"
                     /locus_tag="QBA20_004073"
     CDS             complement(1387..3378)
                     /gene="tkt"
                     /locus_tag="QBA20_004073"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDQ6294398.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVILDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKQAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3656..4414
                     /gene="loiP"
                     /locus_tag="QBA20_004074"
                     /gene_synonym="yggG"
     CDS             3656..4414
                     /gene="loiP"
                     /locus_tag="QBA20_004074"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDQ6294399.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4620..5540)
                     /gene="speB"
                     /locus_tag="QBA20_004075"
     CDS             complement(4620..5540)
                     /gene="speB"
                     /locus_tag="QBA20_004075"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDQ6294400.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWSFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5676..>6055)
                     /locus_tag="QBA20_004076"
     CDS             complement(5676..>6055)
                     /locus_tag="QBA20_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294401.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1492 a         1576 c         1563 g         1424 t
ORIGIN      
        1 ttgctgaatg tataactgag attgatgatt ctggaaaata atcactaagc cgcataaatt
       61 atccttattt tatttttagg gtaccgtccg gcggtaaaaa taaaatactc gtgcggttta
      121 atgaaagcaa ttgggtacgc gagaagaaaa accccacaaa ccaaaataat aaaaccttaa
      181 ataacaacaa tatgaaattg atatttttaa agtcaacttt gtatttgtta tattcatcac
      241 aaaatcaaca ttttgtttgt tattgtattt gtgatgcata tctctattta tttattgaat
      301 gttcgaaaac atacttttct aatgcaatgt tttttcgcta taccccttta tagattctga
      361 attctcacct gtcggctgtt ttctcctcat ctcatgtgac atattcccat actttactca
      421 aggaagattg tcatgaaagc acctgtttca ttaacctcat ttcgcccaca aaaatctctt
      481 gctatcgcca ttggcgtgct tgccgtggtt gtgctgccat ttctctcgta ttacaccgtt
      541 aatgaaggtg aacgcggcat tttgctgcgc tacggaaaaa tcgtaaaagt tgccgaaccg
      601 gggctgggtt ttaagatccc ttttatggaa agcgtggaga aaatttccac gcgtaatcag
      661 gctgtggtct atcagggatt acaggcctac agccgcgacc agcaaccggc acaaatgaca
      721 gtatcagtca gcttccacat taaaccttca gaagcaggcg cggtttatac cacctacaac
      781 actattgaag cattaaaaga tcgtctaatt gtccgtcagt tgccaactca gctggagaac
      841 attttcggcc aatataccgc catcagcgcc gtacaggatc gtacaaaatt ggttcaggat
      901 ctgcaaaatg ccatgcgcaa agccgttgtt gggccagtgg taattgacgg ggtccagatt
      961 gaaaatattg atttttctga cgcttatgaa aagtcgattg aaaaccgaat gaaagcagaa
     1021 gtcgccattg ccacgcgtaa gcaaaatctg gaaacagaga aaatccaggc gcaaatagcg
     1081 gtaactcagg cccaggcaga agcagacagt aaactggcgg cagccaaagc agaagcggaa
     1141 actattcgcg taagaggcgc ggcagaggct gaaactattc gtttaaaaag tgcggctgaa
     1201 gcagaagcaa tccgcctgcg cggtgaagct ctgcgggata atcccggact tgtagccctc
     1261 accactgccg aacgttggga cggtaaactg cccgatacca tgatccccgg cagcacagtt
     1321 ccgtttatct ctactaagta aaaaaagggc cgctttcgcg gtccttctca caaaacgtct
     1381 tactgattac agcagcgctt ttgctttcgc aacaacgtta tcaacagtga agccgaactc
     1441 ttcaaacagc agctctgccg gagcagattc accgaaggtg gtcatgccga cgatagcgcc
     1501 gttcaggcca acatacttgt accagtagtc agcaataccc gcttctaccg caacgcgtgc
     1561 agtaaccgct ttcggcagta cggattcacg gtaagccgca tcctgcttgt caaatgcgtc
     1621 ggtagacggc atggacacca cgcgcgcttt cacgccttcg gcagtcagtt tttcgtaggc
     1681 agcaaccgcc agttcaactt cagaaccggt ggcgatgaag atcagttccg gctgaccagc
     1741 acagtctttc agcacataac caccgcgcgc gatgtttgcc agctgctctt cagtacgttc
     1801 ctgctgcgcc aggttctgac gggagaggat aagcgcagtc gggccgtcct gacgctcaac
     1861 gccgtatttc cacgcgaccg cggattcaac ctggtcacac ggacgccatg tagacatgtt
     1921 cggggtcacg cgcagagaag cgacctgctc taccggctgg tgagtcgggc catcttcgcc
     1981 cagaccgatg gagtcgtggg tgtaaaccat cacctggcgc tgtttcatca gcgcagccat
     2041 acgtacggcg ttacgtgcgt attccacgaa catcaggaag gtggaggtgt acggcaggaa
     2101 gccaccgtgc agggagatac cgttagcaat cgcggtcata ccgaactcgc gaacaccgta
     2161 gtggatgtag ttacctgcag catcttcgtt gattgcttta gaaccagacc acagggtcag
     2221 gttagacggt gccaggtcag cagagccgcc gaggaattca ggcaacagcg ggccgaacgc
     2281 ttcgatagca ttctgcgacg ctttacggct ggcgattttc gccggattag cctgcagttt
     2341 agcgataaac tctttcgctt tggcgtcgaa gtcagacggc atttcgcctt tcatacggcg
     2401 ggtaaattca gccgcttcct gcggataagc tttcgcgtaa gccgcaaact tctcattcca
     2461 tgcagattct ttcgcctggc ctgcttgttt cgcatcccac tgagcataga tttcagacgg
     2521 gatttcgaac ggtgcatatt tccagcccag ctgttcgcgg gtcagggcaa tttcagcgtc
     2581 gcccagcggc gcaccgtggg agtcgtgagt accggctttg ttcggggaac cgaaaccgat
     2641 gatggttttg cacatcagca gggacggttt gtcagtcact gcgcgcgctt cttcaactgc
     2701 gcgtttgatg gatgccgcgt catgaccgtc gatgtcgaga ataacgtgcc agccgtaagc
     2761 ttcgaaacgc attgctgtgt cgtcggtgaa ccagccttca acgtgaccat cgatagaaat
     2821 accgttgtca tcgtagaacg caatcagttt acccagcttc agcgtacccg ccagagagca
     2881 aacttcgtgg gagatgcctt ccatcatgca gccgtcgccc atgaaggcgt aggtgtagtg
     2941 gtcgacaatg tcgtggcccg gacggttaaa ctgcgccgcc agcgtttttt ctgcaatcgc
     3001 cataccgact gcgttggcaa taccctgacc cagcggaccg gtggtggttt ccacaccagc
     3061 ggtataacct acttccgggt ggcccggagt tttagagtgc agctgacgga agtttttcag
     3121 ttcttccatc ggcagatcgt aaccggtgag gtgcagcagg ctgtagatca gcatggagcc
     3181 gtggccgttg gacagcacga agcggtcacg gtcagcccag gacggattct gcgggttgtg
     3241 tttcaggaaa tcacgccaca ggacttcggc aatgtcagcc atacccatag gggcacccgg
     3301 gtgaccggat ttggctttct gtactgcgtc catgctcagc gcacgaatag cattggcaag
     3361 ctctttacgt gaggacattt tgactccaga tcggatgatg aagggcacgc ccttaacgac
     3421 ttgacgacag cgcgttttgg gctacgccgg aaaatttgcc aacaatttac cgcaagccgc
     3481 gcgtcatgta catggaacat ccttttgccg cttcagaaat ctctggatca tgctcgcatg
     3541 ttgcgcaatc tgctcgcccg tccgctgcgc ttttccttat actgagactg agcgtcgatt
     3601 cacctgcaaa cggcgcattt ttagaataat cctgaccttg tgcggaagag aaaacatgaa
     3661 aattcgcgcc ttattggtag caatgagcgt ggcaacggta ctgaccggtt gccagaatat
     3721 ggactccaac ggactgctct catcaggagc ggaagctttt caggcttaca gtttgagtga
     3781 tgcgcaggtg aaaaccctga gcgatcaggc atgtcaggag atggacagca aggcgacgat
     3841 tgcgccagcc aatagcgaat acgctaaacg tctgacaact attgccaatg cgctaggcaa
     3901 caatatcaac ggtcagccgg tgaattacaa agtgtatatg gcgaaggatg tgaacgcctt
     3961 tgcaatggct aacggctgta tccgcgtcta tagcgggctg atggatatga tgacagataa
     4021 cgaagtcgaa gcggtgatcg gtcacgagat ggggcacgtg gcgttaggcc atgtgaagaa
     4081 agggatgcag gtggcgcttg gtacaaatgc cgtgcgagta gctgcggcct ctgcgggcgg
     4141 gattgtcggc agtttatctc aatcacaact tggtgatctg ggcgagaaat tagtcaattc
     4201 gcaattctcc cagcgccagg aagcagaagc cgatgattat tcttacgatc ttctgcacca
     4261 acgcggcatc agcccggcag gtcttgccac cagctttgaa aaactggcga agctggaaga
     4321 aggccgtcaa agctcaatgt ttgacgacca tcctgcatcc gccgaacgtg cccagcatat
     4381 tcgcgatcgc atcagcgcgg atgggattaa gtaaagccta gtggtgtcga acgtaggtca
     4441 gataaggcgt tcacgccgca tctgacatta atggcacatt tgttaccttg tgcgcattgc
     4501 cggatgcgat gctggcgcat cttatccggc ctacgggtgc cgaacgtagg tcggataagg
     4561 cgttcacgcc gcatccgaca ttaatggcac gttttacccg tgcgcatcgc atctggtgct
     4621 tactcgccct ttttcgccgc ctgaatatac agcatctcca gcgccagcgt cgccgcagcc
     4681 agagcggtga tttccgactg atcgtatgcc ggagccactt ccactacgtc catcccaacg
     4741 atgttgagat ctttcaggcc gcgtaccagt ttaatggcgc gatcggaggt caggccgcca
     4801 atcactggcg taccggttcc cggcgcgaaa gcaggatcca ggcagtcgat atcaaaagtc
     4861 aggtaaaccg gcatatcacc cacaatctgt ttcacctggg cgataacgtc atccacgctg
     4921 cgatcgttca cctggcaggc gtccagcacg gtaaagccgt tgtctttatc aaactcggta
     4981 cgaataccaa tctgcacgga atgattcggg tcgatcagac cttctttcgg cgcggtatag
     5041 aacatagtgc cgtggtcaaa ttcacaaccg ttcgcatagg tatcggtgtg ggcgtcaaag
     5101 tgtaccagcg ccattttgcc gaaatgcttc gcatgagcac gcagcagcgg cagcgtaaca
     5161 aagtggtcac caccgaagga gagcatacgc ttaccggcag ccagcagctt ctcggcgtgc
     5221 gcctgcagct tttcgctcat ctcacgggca tcgccaaagg catataccag atcgccgcag
     5281 tccacgacgt tcagacgctc acgcatgtcg aaactccacg ggaagcggtt gtgttcccag
     5341 gccagattcg tcgaaacctg acggatcgct gccggaccgt ggcgaccacc cgcacgacca
     5401 gaagtggcca tatcgaacgg cacgccagta atcacccagt ctgcatcgct gtcatacggc
     5461 tggaagttca tcggcaggcg taaaaaacca aaggcattgg aaaccagtga gttatcgtat
     5521 tgatgaccta aggtgctcat ggatggactc ctattacaaa gtcgatataa aaaaaccctt
     5581 ccgcgtcgtt aggcccgacg aggaagggtt ggatttgcga aacacaagtt tgtgctaatt
     5641 atcgccttaa atgggcgagg catcaagcga ggttgctatt tccgacacgg tggcggcgca
     5701 tagccattgt tatctgccgg aaaggcttgt ccagagcacg gcggcagcgc gttttgcgcc
     5761 gggccttgta attcaaaggc ggcaaagccg attaccccgg tattacgcac atcgccttgc
     5821 gccgaattgg ctgcgtagga atcagcaacg ttggcgaact gaaaagccgc ttcagtatgt
     5881 ttatctttgc ggaatccttt aatcactaac gaatctccgg cattcacaat atagccgttg
     5941 ttattcagcg aaccttgctt gccgttcagc acatccaggc catcagccgt agcaacgatc
     6001 tcgtagttgg tgttacgact gtaattacgg acatataatt gataattcat tccga
//
LOCUS       DAOFMX010000163         5976 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.165, whole genome shotgun sequence.
ACCESSION   DAOFMX010000163 DAOFMX010000000
VERSION     DAOFMX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.165"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(232..1173)
                     /locus_tag="QBA20_004077"
     CDS             complement(232..1173)
                     /locus_tag="QBA20_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm PGA synthesis protein pgaA domain
                     protein"
                     /protein_id="HDQ6294402.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLTDAGHYDTALVKLKQLNS
                     GAPDKANLLTEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIANRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPP"
     gene            1665..2225
                     /locus_tag="QBA20_004078"
     CDS             1665..2225
                     /locus_tag="QBA20_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HDQ6294403.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVS
                     ENTKQRNLFSKKMTLCISLFLFLGGR"
     gene            2168..2557
                     /locus_tag="QBA20_004079"
                     /pseudo
     CDS             2168..2557
                     /locus_tag="QBA20_004079"
                     /inference="COORDINATES: protein motif:HMM:NF028467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
     gene            2537..3094
                     /locus_tag="QBA20_004080"
                     /pseudo
     CDS             2537..3094
                     /locus_tag="QBA20_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            3192..4801
                     /locus_tag="QBA20_004081"
                     /pseudo
     CDS             3192..4801
                     /locus_tag="QBA20_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(4902..5393)
                     /locus_tag="QBA20_004082"
     CDS             complement(4902..5393)
                     /locus_tag="QBA20_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HDQ6294404.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRQYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(5398..5574)
                     /locus_tag="QBA20_004083"
     CDS             complement(5398..5574)
                     /locus_tag="QBA20_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ6294405.1"
                     /translation="MLSPIIEKLQKMEVNCKIHPYIYIATNDSMSGFYAFTCSQMLNS
                     ESPNNCNSLYYKAE"
BASE COUNT         1768 a         1035 c         1245 g         1928 t
ORIGIN      
        1 cgtgcccatt aaccgaagga aaggcggcga agtattaatg gtatgttggg tgacagttag
       61 cgcgcccgga taattttcac tctctaggtg gctgtaaaag agatccgcaa gttcttcatc
      121 ggataaatcc ggggcaatgg tctccttgtg ataaaagagc tcggtcatta ttgactgtgc
      181 ttttttcggc tgatgatctt tgagataagc cgatgcaacc caatattgcc cctagggcgg
      241 aataatttgc cccgtctttt ttaatcgctg atagtgagaa atgacgtctt tataacgatc
      301 gcgagttaat aacgcgccaa gatgatcaac ctgaatacgc tggtactggg cagtgcggtc
      361 tgggttatcg tgccacagaa tttctaatgc agcgtattgg gcgagggcgc gattggcaat
      421 ggcataacgt tcactttcac tgcgcgtagg cataaacgac agtctgacca gttcggcatg
      481 aatatcagcg cgaatatctg gcgttaaatt ggcatcgtca atcgcggcag caagttgatt
      541 attacgtaat gcctgcacgt attctgtggg atattgttgc gtagatgcat tttcaggtaa
      601 tgactctgtc atcgcccgta attcatcctg atgacgcccc gccagtttat agatataggc
      661 ttctgtgagt aaattggctt tgtccggtgc tccagagtta agctgcttaa gtttaaccag
      721 cgcagtatca tagtgacccg catctgtcag ggttaaaatt tgtccccgtt gataatcctt
      781 attttgcggc tccagagaga gcgccttttg ccacagtgta agcgagtttt gccattgttg
      841 caggttacga taagcgacgg cgacagctgc ataaccacgc gctggtaatt gctgatgacg
      901 gtagcggttg taaacggtaa taacctgttt atcttgcccg gcccataagg caatctgtaa
      961 ccagtcagca atttggttat tgctgagtgc tgattttagt gcaaaccatg acaaagctgg
     1021 ctgagtatta cccttgcgag cttcaataat caatgcatca taggcgttat taacagcact
     1081 cttcgccgcg ggactcgctg ctaaaaatgc ggcagtaaga agtttcaaag cccatttggt
     1141 tttcgggcac ctttttctgc tacttgaata catcctgtat tactccatgt attgccaaaa
     1201 tctctctctg tatctaatta caggtaactg aaaagaaaga tatttttgca cctcataatc
     1261 cgttattaaa cgcggaagag agacgtgaat tgttgatgat gagaagaaga aatgatgagc
     1321 agagtgtcca tataaaatcc ttttctcgcc cgaaaatcca ttccaatgat gaggatcttc
     1381 aggaatacgg cataaatccc aatgcctttt tcaaaataaa ttaggattaa aataattaaa
     1441 tcagtaaatt ccgatgcatg atttcggatt tttcgaaagt cctgatgaaa ggctgcgcta
     1501 cggcactcac ggatttaatt gttattccta atgtatctat gaattaatgt tttataagga
     1561 ttttcaagcc tagcttgttg caaacaatgt ataaagcact taggcaataa taattacatt
     1621 cagcaactat catcatcggt attgtttgtg ggcggaattt catattgaca acagtacagt
     1681 tcttatatct attaataata gaaagagatc tacaacctac agattggtgt agctttatgg
     1741 aaaaagacta tttgagaatt agtagtactg tattagtgag cttattgttt gggcttgctt
     1801 tggttttggt gaatagttgg tttaatcagc caggcgttga ggaagttgtt ccacgatcaa
     1861 cctatctgat ggtaatgatt gctttatttt ttatcgatac tgttgcattt atttttatgc
     1921 agttgtattt catttatgac cgtaggcaat tttcaaactg tatacttagt ttggcttttc
     1981 tgagttgttt gatttacttt gttaaaaccg tcattatcat tcagcaaatt attgaggggc
     2041 gtttgacaag cagtgttgtc caaaatgaca ttgcaatcta ttatttgttt cgtcagatga
     2101 gtttgtgcat attaatattt ctggcattgg tgaataaagt tagtgaaaac acaaaacagc
     2161 gcaatttatt ttccaaaaaa atgactttgt gcataagttt gtttttgttt ttggggggcc
     2221 gatagttgct cacatacttt ctagtcatta tgagagctac aatttacaca ttgccgagtt
     2281 aaccaatgaa aatggtcagg tggtttggaa agcctcatat gttaccataa tgattttcat
     2341 gtggttaacc ttgctttcgg ttaatctata ctttaatggt ttacgctatg atatctggaa
     2401 tggagtgacg gtgattgctt tttgtgccgt actgtacaat atctctctgt tatttatgag
     2461 caggtatagc gtgtccactt ggtatattag tcgcactatt gaagtagtca gtaagttaac
     2521 cgttatggta atatttatgt gccatatatt cagtgcgcta cgagtaacaa agaacattgc
     2581 acatcgcgat cccttaacca atatatttaa cagaaattat ttttttaatg aactgacagt
     2641 tcaatcagca tcagccaaaa aaacgcctta ttgtgtcatg attatggata tcgaccactt
     2701 caaagaagtc aacgacacct gagggcatcc ggttggcgat caggtgataa aaacagtggt
     2761 gaatatcatt gggaaaagca tacgaccaga tgacctttta gcgcgcgtcg gcggcgaaga
     2821 gtttggcgtc ttgctgacgg acatcgatac tgaacgtgcg aaagctttag cggaaaggat
     2881 tcgggaaaat gttgagcgtt taactggcga taatcctgaa tacgctatac cacaaaaagt
     2941 gacgattagt attggcgctg ttgtgactca ggaaaatgcg ttaaacccga atgagatcta
     3001 tcgactggct gataacgcac tatacgaggc gaaagagacc gggcgtaata aggtggttgt
     3061 gagggatgtg gtgagttttt gtgagtcacc ataaagcggc attttagcgg gtgattttgg
     3121 taagcgctaa atagctattc gcagacttgc gtaacaattt tcagaaatat catagtacta
     3181 tatgatctta aatgcttatc attgatcaat ctgctatcac ccgcttttac tggcaattgt
     3241 actataattt cttaggttgc atcaccctga tgcgtgggga aacatgtatg gtattcaaca
     3301 aaaaaatgtt tgtactcatt ataataccag gcattctggg ggtgttacta tcgtttgcta
     3361 tgtcaatatt tcaaatgaat agagatacga caattaccgc cggtattctg ttaaaacagc
     3421 ttgataatgt tacgcaaatt gtccagcata cgacaaagct tacctctatt ctggtgatga
     3481 agccctgcaa agacattctt gaacagttga ttgctaacgg agcattgact ccctatgttc
     3541 gaacaacggg tcttatagaa aataatttcc aaatatgttc ttcagttagc ggtttcaaaa
     3601 aaatgaatgt taacgatgtt tacggtacga gctttcacaa taaaaataaa gaatcacgta
     3661 tagtatcaat tagcggtaca agttttgtgc caggtaaaac agcaatagtg ttccttatgc
     3721 caatagggaa tgatatgaca gcttttagta ttgtagagtc acgatatatc tatgatctaa
     3781 tggatgttct tgatgatgag aatgatgatt cattttctct tcgttttact gaagggcctg
     3841 caattattag cggtgtaaat aataatgaca ggttatatat gttaaaaaga gattttaatt
     3901 ctgcgatcag tcaggctcgt cttacagtta ctacgccgat gatatcgtta tatccctata
     3961 tcattagcaa tgtgctttat attttgccgt tatctattat tctctctttt attctctatt
     4021 tcttatggca gcactggatg tcacggaaaa tgtcgctggc agaagaaatt aaaaaaggaa
     4081 tgtcatccgg agaattttct gtgcattatc aaccagtgtg cgacacgaca accaaagcgt
     4141 gtctgggggt tgaagcgtta atgcgctggc aacgcgaaga tggtaaaaat atatctccgg
     4201 tagtttttat ccgcgctgca gaagaggaaa atttaataat accgttaact aaacatctct
     4261 ttgaactgat tattcaggat gtacaaagct ggaaagtgaa aaagccgttt caccttggga
     4321 taaatattgc cgcggaacat cttgcacatc ctgactttgt tggtgatgtt ttacatatta
     4381 aaaatgcaat atctgataaa tttaatattg tccttgagat taccgaacgc aatctggttg
     4441 aagacactga tcttgcatta cagaaaataa atgaattaag gatccatggc tgtgaatttg
     4501 cagtcgatga ttttggcacg ggatattgtt cccttggcct tctgcaaaag ttgtctgttg
     4561 attatcttaa aatagataaa agctttatag acacacttac tacagcggaa gacgaaacgc
     4621 cagttttaga tattattatt aagctgagta atcggctcaa tttgataacg attgcagaag
     4681 gtgtcagtac tcctcaccag cagaatatct tattcataat aacgtgacat tagttcaggg
     4741 atacttatat gaaaaaccaa tgaaagcgac agagttttat cagtggtatg taaacagata
     4801 aataattaaa aatattggaa tggttttgta ttgtcttaaa atataaacaa agcaatcaga
     4861 atgttattaa ctgatgacaa acgccaggat caacaatgct gttagtgagt aatacatatg
     4921 aatcgtggtg tcctgcctgt agagaatatg tagttataat cattttgacg cgtaacataa
     4981 taattaattg aagtgtctgc ttttatgaag aaatcagaag tgatattttc aaggatttca
     5041 tcgctgatgt tatcatcttt ttgtattttt tggattttaa ctgtggtgag ataaacggta
     5101 tcacggaatt tcttgtatgt aaaactcaca tattggcgga tgaagcccat tttttttcca
     5161 tctaccgtgt atgcgcccgt gagatacatg tttcctgtgt tattttcaga aatcatattt
     5221 aacgaaaaat attgatccat ttttgtatga tcggtgaaat taataacatg ggcattacat
     5281 gatatgtttt gctctgctgg ttcattggaa tgatgaaacg caatatggct cagaattgct
     5341 gcaataaaag cacataaaat tattcccgaa aacagaaaat atctttgagt catatgatta
     5401 ctccgcttta taatagagag agttgcaatt atttggtgat tcagagttta acatttgact
     5461 acaggtgaaa gcataaaacc cgctcatgct atcatttgtt gcaatgtaaa tgtaaggatg
     5521 tattttgcag ttgacttcca ttttctgcaa tttttcaata attggtgata gcatgtctac
     5581 ggaattaaag taattattat catagaaata actgcatcct tcctgagtag ggtgttgagc
     5641 ataatttgag aaagcatcta ttttttttat agtgtgagat ttgctttgaa aagccatatc
     5701 gaaaaaataa gaagacatat aaactatccc ccatgccgca aaaaaaaact ataagtaatg
     5761 tattctttgt ggtaaatata ccttttgcaa cgtaattttt atttatattt tcagtgtggg
     5821 tgccattctc ttcactctca acactttcat tctcattgtg attattgtcg agaacagtta
     5881 tgttgatttc ttcattgatt ttgaagcctt tccgtggcac cgttctgata actgatccta
     5941 cccacgtaat atggacacag gcctaagcga ggttct
//
LOCUS       DAOFMX010000164         5890 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.166, whole genome shotgun sequence.
ACCESSION   DAOFMX010000164 DAOFMX010000000
VERSION     DAOFMX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.166"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            50..244
                     /locus_tag="QBA20_004084"
     CDS             50..244
                     /locus_tag="QBA20_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDQ6294406.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
     gene            511..1437
                     /gene="tauA"
                     /locus_tag="QBA20_004085"
     CDS             511..1437
                     /gene="tauA"
                     /locus_tag="QBA20_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6294407.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="QBA20_004086"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="QBA20_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDQ6294408.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="QBA20_004087"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="QBA20_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDQ6294409.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLDASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="QBA20_004088"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="QBA20_004088"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDQ6294410.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="QBA20_004089"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="QBA20_004089"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDQ6294411.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5493..5861
                     /locus_tag="QBA20_004090"
                     /pseudo
     CDS             5493..5861
                     /locus_tag="QBA20_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1399 a         1455 c         1598 g         1438 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg gatgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttggttatt
     5161 attttttggc gtttggttgt gctgctataa attgaataat tcaaactttg ttgtatattg
     5221 ataagatatt tatctggttt gtgtttttga tatttataac gcgtatgcat caaaaataac
     5281 attatctgca acatactgaa atatcgtcct tttatcaatt tgatgcgtgt ttttctccat
     5341 aaattatata tgtccacatt cggacttagg ggaaagaata attgaaccat tcgtctgtaa
     5401 cgcagcataa tcgttagcgc gaaacataat atgttttcta tgcattgata attgatggat
     5461 caacttatta cgtccctgag gagggatgac aaatgcactc ctggaaaaag aaacttgtag
     5521 tatcacaatt agcattggct tgcactctgg ctatcacctc tcaggctaat gcagcgacca
     5581 acgatatttc tggtcaaact tacaatactt tccatcacta caacgacgcc acctatgctg
     5641 atgacgttta ctatgatggt tatgtaggct ggaacaacta tgccgctgat agctattaca
     5701 acggcgatat ctacccggtc attaataacg ctaccgttaa cggcgtgatt tctacctact
     5761 atctggacga cggtatttct accaatacca acgccaatag tctgacaatc aaaaacagca
     5821 ctattcacgg tatgatttat tccgagtgca tgacaactga ttagactggc cccctgaatc
     5881 tccagacaac
//
LOCUS       DAOFMX010000165         5840 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.167, whole genome shotgun sequence.
ACCESSION   DAOFMX010000165 DAOFMX010000000
VERSION     DAOFMX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.167"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(52..531)
                     /gene="xdhC"
                     /locus_tag="QBA20_004091"
     CDS             complement(52..531)
                     /gene="xdhC"
                     /locus_tag="QBA20_004091"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDQ6294412.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CRFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="QBA20_004092"
     CDS             complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="QBA20_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDQ6294413.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="QBA20_004093"
     CDS             complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="QBA20_004093"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDQ6294414.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4129..4884
                     /locus_tag="QBA20_004094"
     CDS             4129..4884
                     /locus_tag="QBA20_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDQ6294415.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4963..5036
                     /locus_tag="QBA20_004095"
     tRNA            4963..5036
                     /locus_tag="QBA20_004095"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4995..4997,aa:Gly,seq:ccc)
     gene            5258..5803
                     /locus_tag="QBA20_004096"
                     /pseudo
     CDS             5258..5803
                     /locus_tag="QBA20_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1471 a         1432 c         1481 g         1456 t
ORIGIN      
        1 ttaaacacat aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt
       61 tttctcgcaa tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt
      121 tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag
      181 cattgccgtg gtagccataa tcaggccagg cgtacaaaac ctgcactgca ctgtgccgga
      241 tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt
      301 gcggatctct tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc
      361 gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag
      421 cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa
      481 aggcatcccg ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt
      541 ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc
      601 agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat
      661 tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca
      721 tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa
      781 ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg
      841 cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga
      901 aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca
      961 ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt
     1021 agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta
     1081 gcgacgttac ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga
     1141 tgacgttgag ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt
     1201 agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca
     1261 atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg
     1321 agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg
     1381 cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa
     1441 ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat
     1501 cttcaccgcg ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt
     1561 atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag
     1621 atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt
     1681 agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg
     1741 tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat
     1801 gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc
     1861 aaacgccggt gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg
     1921 ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg
     1981 tcgttcaacc agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag
     2041 catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc
     2101 aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc
     2161 ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac
     2221 catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt
     2281 gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca
     2341 ggtgttagag gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg
     2401 gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca
     2461 ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg
     2521 ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc
     2581 aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc
     2641 cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag
     2701 gtaagcgact ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc
     2761 ggtgttagac agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat
     2821 ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct
     2881 aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc
     2941 ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac
     3001 gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg
     3061 ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct
     3121 ttccatgtga caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc
     3181 ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc
     3241 gccgttatgg attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg
     3301 taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg
     3361 ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg
     3421 atcggcggta tcgcgcttgt tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg
     3481 aatatcaggc acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg
     3541 ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta
     3601 acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat
     3661 ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat
     3721 cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta
     3781 cgccagaaac gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac
     3841 agatatgata cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg
     3901 ataaaatcaa tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag
     3961 tcaggaactt cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat
     4021 gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga
     4081 ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga
     4141 cgcctgaata aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg
     4201 ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg
     4261 aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg
     4321 gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg
     4381 ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc
     4441 gcatcggtta caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt
     4501 tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc
     4561 aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga
     4621 aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac
     4681 agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa
     4741 agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc
     4801 ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg
     4861 cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg
     4921 ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg
     4981 tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccaattt
     5041 atcttcacct gatcaatctg ccatttttgc tgtatctgat tttctatatt atcttaattc
     5101 aatcacttca ggcttttcgc aatcgctgta acacttcccc atttttttgt aaataaaaag
     5161 cgtttacgca atgcaatcct tttgtaacaa cccaggtaaa ataccttatt ttcaagttgt
     5221 tgaataagtg gttatctacg cttttaacaa acgattgatg gattatttta tgaaagggaa
     5281 atctgcatta actcttctgc tcgctggtat attttcgtgc ggcacatgcc aggcgacaga
     5341 ggcagaagtg acaagcgaat ctgtatttaa cattcttaat tccagggggg cagcaacaga
     5401 taaaagttat ttatcgttaa atccagataa gtatcctaat tatcgtcttc tgattcactc
     5461 agccaaatta aaaaatgaaa taaagagtca ctataccaaa gacgaaactc aaggattgct
     5521 aacattaaca gaaaacacac gaaagttaac gttgacggag aaaccctggg gaacgtttat
     5581 tcttgcttct actttcgaag atgacaaaac agcggcagaa acccactatg atgctgtctg
     5641 gctgagggat agtttatggg gctatatggc attagtatca gaccaaggaa atagtgtagc
     5701 cgcaaaaaag gttctgctca ccctgtgggg ttatatgtct acgcccgacc agattaagcg
     5761 gatgcaggac atcattagca acccaaaacg actggatgga attctgatcc tacccacgta
     5821 atatggacac aggcctaagc
//
LOCUS       DAOFMX010000166         5783 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.168, whole genome shotgun sequence.
ACCESSION   DAOFMX010000166 DAOFMX010000000
VERSION     DAOFMX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.168"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..940)
                     /gene="hprS"
                     /locus_tag="QBA20_004097"
     CDS             complement(<1..940)
                     /gene="hprS"
                     /locus_tag="QBA20_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDQ6294416.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLN"
     gene            complement(940..1611)
                     /gene="hprR"
                     /locus_tag="QBA20_004098"
     CDS             complement(940..1611)
                     /gene="hprR"
                     /locus_tag="QBA20_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDQ6294417.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1744..2157
                     /gene="hiuH"
                     /locus_tag="QBA20_004099"
     CDS             1744..2157
                     /gene="hiuH"
                     /locus_tag="QBA20_004099"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDQ6294418.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2266..3270
                     /gene="msrP"
                     /locus_tag="QBA20_004100"
                     /pseudo
     CDS             2266..3270
                     /gene="msrP"
                     /locus_tag="QBA20_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3271..3906
                     /gene="msrQ"
                     /locus_tag="QBA20_004101"
     CDS             3271..3906
                     /gene="msrQ"
                     /locus_tag="QBA20_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDQ6294419.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4164..4814
                     /gene="zinT"
                     /locus_tag="QBA20_004102"
     CDS             4164..4814
                     /gene="zinT"
                     /locus_tag="QBA20_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDQ6294420.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1642 a         1306 c         1195 g         1640 t
ORIGIN      
        1 tattgagaga aagcgaatcc agtttcacca aaacattgtt tttgtctgcc ctggcaagaa
       61 acagtatatt ctctgttaac cgcgaaatat tttccagctc ttcaatgttt ccggcaattg
      121 ttttttgata ttcagcgata cttctgattt gactgagcgt aacctgattc tgacccagta
      181 atgcattaat tggcgttcta agttcatgag cgagatcgtc agcaaattga ctaaggcgct
      241 caaaatcttt gactaaagca tgatgcattt tattcaacgc ctgcccaaga ggttttagtt
      301 ctctcggtaa tgcgttaacc tcaaccggca ctcggctatc gttataattc agcgcttccg
      361 ttacgccact caactttttg atctctcgca accccgtcct gattaacaac ggacttaata
      421 ctgagcacag tataatggcg acaatgcaaa ttataatgct attaatttta tactgttcaa
      481 gcatattatg tctggctgaa gccaatttag ccacagtaac cgttaatgat ggcgaaactt
      541 catcaatgtt aattcgtaaa gcatctatct ctgtatcatt aataatgctt ctgtaaatgc
      601 ctgctgcgct gattgtctca ctagcaggta tgttatttaa catgtcatca ctgacatttg
      661 tccggttaac aattttattg atgccatcac tatgaatgat caaaatatcc tgactaacat
      721 ccatcatccg gttaaagtac acaggtaacg tatctggatt taccccatca attaacaact
      781 gcttgatctg cgctgtccgg ttaatgagtg ttgtatcatc gcgccatttc aactcacttg
      841 ccaggccatt atagagagtc catacaattc cagcgccagc aacagactgc agaaatataa
      901 aaagcaaggt taaacggacg gttatagata atcttttcat tattttttta ccgctacgaa
      961 tgaatagccc atcccccgga ttgtggcgat tagcttttca ggaaaaggat catcaacttt
     1021 tgcgcggagc ctgcgaatgg cgacatccac cgtattggta tcactatcaa agttgattcc
     1081 ccaaatttca ctcgcaataa ccgttctggg tataatctcg ccagctctgg aggccagtag
     1141 ccaaagtaac tgaaactcct tgcgcgtcag tgtaatactg atattgtccc tgctgacact
     1201 ttgactgaca gagtccattt ttaagccact gatttctaat gttgaattca gagcgtgatg
     1261 ttgccttaat tgtgcccgaa cccttgctag caactcagaa aatgaaaaag gttttaccag
     1321 ataatcattt gccccactgt ccaaccctct gactctgtca tcgacagaat cccttgcagt
     1381 aaggcaaata acaggggttt gctttgccgt tcttaacgtt tgtaagatct gccagccatc
     1441 catacccgga agcataatat ccagaatgat caatgcataa tcatccttca gcgcaagata
     1501 aagcccatct ctgccatcag aaacggcatc aatgacataa cccgcttcgg aaagcccctg
     1561 cgttacccat tcctgggtcc tttgattatc ttcaataagt agaatcttca tgaaaataat
     1621 atgccataaa tcatatgtta tagaaacaac ctggttcatt acaaaattgt aatgctgctg
     1681 taaggttacc ctggacgttt tttcgctatc ctcaaaactc attcacatga caaggatata
     1741 aacatgttaa agcgttattt agtactctcg gtagcaacgg cagcattttc attaccttct
     1801 ttggtttatg ccgcacaaca aaacattctt agcgtgcaca ttttgaacca gcaaacagga
     1861 aaacccgctg ccgacgtgac agtcactctt gaaaagaaag cggataacgg ctggttacaa
     1921 cttaataccg ccaaaacaga taaggatgga cgaattaagg cactgtggcc cgagcaaact
     1981 gcgactactg gcgattaccg tgtcgtattt aaaaccgggg actatttcaa gaaacaaaat
     2041 cttgaaagtt tcttccctga gattcccgtt gaatttcata ttaataaagt gaacgagcat
     2101 tatcatgtgc ctttgttact cagccaatat gggtattcaa cctatcgtgg cagttaatgc
     2161 gtaaatatgt aaaagctgat ctctgctact gttagggagg tgactccctg acagattaac
     2221 agtaaacagc tcttgttggt taacgacaaa aaagtgtgat ggcttatgaa aaagaagcaa
     2281 tttttaaaag aatcagatgt tacggccgag tcggtattct ttatgaagcg tcggcaggtg
     2341 ttaaaagcac tgggcatcag cgcagctgca ttttctttgc ctcacgctgc gcatgccgat
     2401 ctgcttagct ggtttaaagg gaacgatcgt ccgcccgccc ccgccggaaa accgctggag
     2461 ttcagcaagc ctgccgccta gcaaaataac ctgccactga cgccagcaga taaagtctcc
     2521 ggttataaca acttctatga attcgggctg gataaagacg atcccgcggc taatgctggt
     2581 agcctgaaaa ccgatccatg gacactgaaa atcaacggcg aagtggcaaa accattgacc
     2641 ctcgatcatg atgatttaac ccgtcgcttc ccgctggaag agcgtattta tcgtatgcgc
     2701 tacgtggaag cgtggtcgat ggtggtgccg tggattggtt ttccgctaca caaattgctg
     2761 gcgcttgccg aacccaccag caatgcgaag tatgtcgctt tcgaaacgat ttatgcgccg
     2821 gaacagatgc ctggccagca ggaccgcttt atcggcggcg ggctgaaata tccttatgtc
     2881 gaaggattgc gtctcgacga agcaatgcat ccgctcacac tgatgaccgt aggtgtttat
     2941 ggcaaggcgt taccaccgca aaatggcgcg ccggtgcgac tgattgtgcc gtggaaatat
     3001 ggctttaaag ggattaaatc gatcgtcagt attaagctga cccgcgagcg tccgccaacc
     3061 acctggaatc tggcagcgcc tgacgaatac ggtttttacg ccaacgttaa tccgcatgtt
     3121 gatcacccgc gctggtcaca ggctaccgaa cgatttattg gttcaggcgg cattctcgat
     3181 gttcagcgcc agccaacact actgtttaat ggttacgccg accaggtggc atcgctgtat
     3241 cgtggcctgg atttgcggga gaatttctaa atgcgtctga cagcaaaaca agtgatatgg
     3301 ctgaaagttt gcctgcatct tgccggattg ttgccgtttc tctggctggt ctgggcgatc
     3361 aatcacggtg gactgggtgc cgatccggtg aaagatattc agcattttac cggtcgcact
     3421 gcactgaaat ttttgcttgc ggccttgtta atcacacctc tggcacgcta cgcaaaacag
     3481 ccgttattga tacgcactcg ccgcctgtta ggcttatggt gctttgcctg ggcgacactg
     3541 catttaacca gttacgcatt gctggagttg ggcgtgaata atctggcgtt attaggtaag
     3601 gagttaatta cccggcctta tttaacgtta ggaattatca gctgggttat tttgcttgct
     3661 ttagcgttca cttcaaccca gtcgatgcag cgaaaactcg gcaaacactg gcaacagttg
     3721 cataacttcg tctatcttgt cgcgatcctg gccccaatac attatctgtg gtcagtgaag
     3781 attatttcac cgcagccact catctacgct gggctggctg tactgctttt agccttacgg
     3841 tataagaagt tgctttccct tttcaaccgg ttacgcaagc aggcccacaa caaattatct
     3901 ctataacttc atattgccga caaacttcgc gttatcccgg ccggatgcgg cgcgagcgcc
     3961 ctatccggcc cacacagatc gcatcaagtt atgtttcgtt tgtcatcaac ctttcatcac
     4021 cagaataatt tattttattt caataagata gttcaataca cctgagaata ccatgctctc
     4081 atttcctaat gagttgttgc attttttcat atgttacaat ataacattac acataatgta
     4141 cattaactct ggaggaaatt gttttggcga ttcgtcttca taaactggct gttgctttag
     4201 gtgtctttat tgttagcgct cctgcctttt cgcatggtca tcattctcat ggcaaaccct
     4261 taacagaggt cgaacaaaaa gctgctaatg gtgtttttga tgataccaat gtacaaaacc
     4321 gaacgctcag tgactgggat ggagtctggc aatccgttta ccctttactg caaagtggca
     4381 aacttgatcc cgtctttcag aagaaagcgg atgcagataa aaccaaaaca tttgctgaaa
     4441 ttaaagatta ttatcgcaaa ggttatccga cagatatcga aatgattggc attgaggacg
     4501 gcattgtcga attccataga aataatgaaa caacatcctg taaatatgat tacgatggat
     4561 acaaaatact cacctataaa tcaggcaaga aaggcgttcg ttacttattc gaatgtaaag
     4621 atcctgaaag caaagcccct aaatatatac aatttagcga tcatataatt gcaccacgaa
     4681 aatcatctca ttttcacatt tttatgggta atgattccca gcaatcttta ttgaatgaaa
     4741 tggaaaactg gccaacgtat tatccctatc agttgagtag cgaagaagtg gtcgaggaga
     4801 tgatgtctca ttgatttttt tgcgctgact tcagttattt aacagaacag tgcctggtag
     4861 taacaacaat aatttaccaa gacaaataag taagacaaac tcagacacgc tcatacttga
     4921 gtcgtgtctt ttttattatg tctgtagaaa aaaactgaga aaaaggctaa caataaatac
     4981 acaactcagc catacagact ggcagcgcaa atgtttccag gagcaagtca tctgtcatgg
     5041 taaagctaac cagaaatata ggttaatttt gctgtttaaa ccttttttac ttgttagtat
     5101 gatacaaagg ctttcaaaaa agctgggaaa ttcaacaact cttggaaatt atggactttg
     5161 tcccgcggct ttaatctgac ggccgcgttc cttttttggc tcaattattg accccttctc
     5221 tacgcaactt cagttcccgc cagcaacttt gcggcagttt cataggatca atgtctaaaa
     5281 gaataatggt gaccgataag aaaacgactg aataactgta gattttcgct cggaaccttc
     5341 ctgtcagctc catagcgaat caagcgctga atgtcacagt atcgaacaga aaacagtgac
     5401 gatctaaccc ttcaagaata ttctacgatt gttctgttta ggaaaagcaa ggcgggaagt
     5461 cgggagataa gtcattgata aaatggcgga gagaggggga tttgaccccc cggtagagtt
     5521 gcccctactc cggttttcga gaccgatgat acagagtcga aaaatcaaag agttaaaaat
     5581 ttttcttgga aaaaatgtct taaaatcgta cacgaaaaat caatgaatta cataacatta
     5641 aagaagaata ttccaagcct tcttgagtcc accacctgtt ttcaggtgtc attcagacgt
     5701 gatttgcccc agattgggaa agcacgcttt tttctcagct tattccgttt tgcctgtgtg
     5761 ttgaataatt ccactgcagt tat
//
LOCUS       DAOFMX010000167         5702 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.169, whole genome shotgun sequence.
ACCESSION   DAOFMX010000167 DAOFMX010000000
VERSION     DAOFMX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.169"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..170)
                     /locus_tag="QBA20_004103"
     CDS             complement(<1..170)
                     /locus_tag="QBA20_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294421.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="QBA20_004104"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="QBA20_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDQ6294422.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="QBA20_004105"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="QBA20_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="QBA20_004106"
     CDS             complement(3616..4383)
                     /locus_tag="QBA20_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDQ6294423.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /locus_tag="QBA20_004107"
     CDS             4579..5487
                     /locus_tag="QBA20_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDQ6294424.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            5484..>5702
                     /locus_tag="QBA20_004108"
     CDS             5484..>5702
                     /locus_tag="QBA20_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDQ6294425.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1411 a         1470 c         1482 g         1339 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gatctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggattgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttattgca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcccaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggca
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAOFMX010000168         5601 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.170, whole genome shotgun sequence.
ACCESSION   DAOFMX010000168 DAOFMX010000000
VERSION     DAOFMX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.170"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..411
                     /locus_tag="QBA20_004109"
     CDS             <1..411
                     /locus_tag="QBA20_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6294426.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="QBA20_004110"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="QBA20_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="QBA20_004111"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="QBA20_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HDQ6294427.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1379 a         1402 c         1332 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacatcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc g
//
LOCUS       DAOFMX010000169         5557 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.171, whole genome shotgun sequence.
ACCESSION   DAOFMX010000169 DAOFMX010000000
VERSION     DAOFMX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.171"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..641
                     /locus_tag="QBA20_004112"
     CDS             <1..641
                     /locus_tag="QBA20_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HDQ6294428.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="QBA20_004113"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="QBA20_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDQ6294429.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="QBA20_004114"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="QBA20_004114"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDQ6294430.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatag
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAOFMX010000170         5431 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.172, whole genome shotgun sequence.
ACCESSION   DAOFMX010000170 DAOFMX010000000
VERSION     DAOFMX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.172"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..497
                     /locus_tag="QBA20_004115"
     CDS             <1..497
                     /locus_tag="QBA20_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HDQ6294431.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="QBA20_004116"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="QBA20_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="QBA20_004117"
     CDS             2500..3114
                     /locus_tag="QBA20_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDQ6294432.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="QBA20_004118"
     CDS             3114..3443
                     /locus_tag="QBA20_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDQ6294433.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="QBA20_004119"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="QBA20_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDQ6294434.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4530..4841
                     /locus_tag="QBA20_004120"
     CDS             4530..4841
                     /locus_tag="QBA20_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDQ6294435.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4976..5375
                     /locus_tag="QBA20_004121"
                     /pseudo
     CDS             4976..5375
                     /locus_tag="QBA20_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1188 a         1347 c         1431 g         1465 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa actatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttatcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 tttttattgt ctgagatcag agatacggct aataaccgta tctcttgaca gagtgagcag
     4441 atgcacataa attaatcata cggttgatag ccgtattatg tatgagatcg actccctacg
     4501 atttcattca gttaatcaaa gaagtcataa tgcctggaaa aacagccgct ctatacgatg
     4561 tcgataaaac gctgaaaaat gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca
     4621 aacttcgtga agctgcgcaa gccgttgggg tagatttaag cgcttttatt ttgagcgctg
     4681 ctatggaacg cgccgaaagt gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa
     4741 gctgggaact gatgaaccaa ctcatcgctg aacctgctca accgacgctc gccctcaagg
     4801 cgttaatgaa aaggaaaaac agcgatggtc gacaagcatg aagagattac tctgcccata
     4861 gtcctctcct gtaattatca gtctgatatt acttatcctg ggcaaaaaca gtttgattgc
     4921 ggtaaccctg ttatcgataa atttgtacgc gctacgcgca tcgctaaaga aaagtgtgcg
     4981 taatagcgac tgtgcggcta aagcacttat tgacagacaa agtggtgaac tgattggcat
     5041 ctgtactttt acggcatatt cgctggaaag acaacgcgtt tcttggcgtc cttcagggtt
     5101 cacaaccttc agaaattggt gttgtcagat tagtcatgtt ggggggagcg cggaagtatc
     5161 aaaagcgggg ctttggtcag gacctactat gtgatttttt tgaacatgta aaaaaaattc
     5221 acccggcatt accaattaaa ggggtttatc ttgatgctga ccctgccgcc attaactttt
     5281 atgctcgtct cggctttgtt cagctttcag cgacaccaaa tgcttttggt gctgtaccta
     5341 tgtttttggc gattcagcat attctcgcgg cttagattat tatcgccacc cgccagggca
     5401 agttttgggt aatgactcca acttactgat a
//
LOCUS       DAOFMX010000171         5336 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.173, whole genome shotgun sequence.
ACCESSION   DAOFMX010000171 DAOFMX010000000
VERSION     DAOFMX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.173"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="QBA20_004122"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="QBA20_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDQ6294436.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="QBA20_004123"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="QBA20_004123"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDQ6294437.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="QBA20_004124"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="QBA20_004124"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDQ6294438.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     AENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="QBA20_004125"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="QBA20_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDQ6294439.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1309 a         1289 c         1457 g         1281 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatgcgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcaacag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcgg
//
LOCUS       DAOFMX010000172         5166 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.174, whole genome shotgun sequence.
ACCESSION   DAOFMX010000172 DAOFMX010000000
VERSION     DAOFMX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.174"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..223
                     /locus_tag="QBA20_004126"
     CDS             <1..223
                     /locus_tag="QBA20_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6294440.1"
                     /translation="SLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFH
                     WGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(395..724)
                     /locus_tag="QBA20_004127"
     CDS             complement(395..724)
                     /locus_tag="QBA20_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDQ6294441.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(729..914)
                     /locus_tag="QBA20_004128"
     CDS             complement(729..914)
                     /locus_tag="QBA20_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDQ6294442.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(911..3550)
                     /locus_tag="QBA20_004129"
     CDS             complement(911..3550)
                     /locus_tag="QBA20_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDQ6294443.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3758..4747
                     /gene="ldhA"
                     /locus_tag="QBA20_004130"
     CDS             3758..4747
                     /gene="ldhA"
                     /locus_tag="QBA20_004130"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDQ6294444.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4858..>5166
                     /gene="hslJ"
                     /locus_tag="QBA20_004131"
     CDS             4858..>5166
                     /gene="hslJ"
                     /locus_tag="QBA20_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDQ6294445.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1306 a         1323 c         1205 g         1332 t
ORIGIN      
        1 tagtttgtac cgaatgtttg ccgataaagg tttggtagtc gcgcaatata ttcgtaaccg
       61 tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa aactggcagg
      121 catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat ttaagcaacg
      181 ctttgggatg acgccaggcg agtatcgacg taaattccgc taactaattg gcgttgcatg
      241 tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag ggcgaaaggc
      301 aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca atcgctttct
      361 cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ctttcgcacc catttaccgt
      421 ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc ccggcgagtt
      481 tcgcgatatc atctacagac acattatttt gctttgcccg ttgttggtaa ctcgctttac
      541 gttcggcatt aatttcggtt actaatttct ctgtttcagc atccgttttc agcgcaacca
      601 gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc agagtcaatg
      661 ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat gttttcttca
      721 tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc tttgtctgcc
      781 ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg ttccttaggt
      841 gcagcgactt caatgcgagg cgtacagcca accagcataa aagatgacgt caacgcagcc
      901 agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca tttcccggca
      961 gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa ctgcgccaca
     1021 gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt ttaccatcaa
     1081 cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta tctaagttaa
     1141 tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct gaacccgcag
     1201 tcatattgtc tttcaccacc gcatctgcgg tatctttatc cagccgcaag gtcattggcc
     1261 cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc cataacggca
     1321 atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg ctaaccagtt
     1381 cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc atacgtaatt
     1441 gtttcatcag cacgttaccg cctaacacat cgacgctaac atcagtcaac agcaagggtt
     1501 cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc gctgtcacca
     1561 gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga tgccaggctc
     1621 catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta tctggcatcc
     1681 aggcactacc gcctttcaac acgccgtgcc ctcccgcgcg gaatccttgt tcaggcgcag
     1741 cagaaaatgc aacctgagca tacagttcac catcgcgtaa gttcatcttc cagtcgggtg
     1801 gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta ccgcgcagac
     1861 gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca tgtaaatcgc
     1921 ctttaaagag gaaataggtc ggattgcgcc catcgacgct aaattttaag gttgatggcg
     1981 gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc gcgccgctga
     2041 aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt aatcgcggct
     2101 tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagaga tcggtcagcg
     2161 taatcgtgct gtcatgccac tcaccttttc ctgcgacatc ccaggtggca ttcatcggtg
     2221 taaaactccc ttttccccag tagcgccact gccagcggcc agcgtcaggg agaaaatcat
     2281 tcgccagccc atccatatgc agcacgaaat cgcccagttc attttcatgc gcctgcaaaa
     2341 tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct aaaggccagc
     2401 ggatttcatc gatgtccagc gaatcgatga ctcttccctt tgaacgaagt aacgcgcctg
     2461 gctcaaaggt cagcgttggg tcagacagac ttccacttag ctgcgcaggt aaacgggcat
     2521 ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagt tgactgttat
     2581 ccatgcttaa ttttcctggg ccaaaattaa gcaccgcgtt gcccttaccc gcttgcccct
     2641 gggtcagcac attcagtcgt ccgctgacca gagcgttctc aagccctgcc tgccagttgt
     2701 cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacagccag ctccagcgac
     2761 catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc aacggatcgc
     2821 cgttatcccg tgccagcacg atcaattgcc cgctattttc ctgccagtcc agctcggcat
     2881 ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca cttacaggaa
     2941 gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc ggctgatttt
     3001 caaacgcgac gacatccagt tcgctgactg taagttgttg ccctttcagc tggccttgaa
     3061 atttaacttt ttcgccctga taacgcagtt gctggatatc agaggttaat gcgagagaga
     3121 gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg atccaggtgt
     3181 taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat tgctccgtct
     3241 gcggcaattt cgccagacaa gcagaatcaa gttctaccgt gccgacgttc aataaccagc
     3301 ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct cccaccagat
     3361 aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca tctaatgcaa
     3421 tacgtgtccc gagcggtagc cagatgtccg ccagcgtggg aacccacagc ccgagcgtca
     3481 tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg gctttatatt
     3541 tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc aatacgggta
     3601 ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcata gttgttgatg
     3661 aatttttcaa tatcgccatg gctttcaatt atatttgaaa ttttgtaaaa tatttttagt
     3721 agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg tttatagcac
     3781 aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct ttgagctgga
     3841 attttttgac tttctgctga cggaaaaaac cgctaaaact gccaatggct gcgaagcggt
     3901 atgtattttc gtaaacgatg acggcagcca cccggtgctg gaagagctga aaaagcacgg
     3961 cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg atgcggcaaa
     4021 agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaggccg ttgctgaaca
     4081 cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc agcgtacccg
     4141 tgacgctaac ttctctctgg aaggtctgac cggctttact atgtatggca aaacggcagg
     4201 cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc attctgaaag gttttggtat
     4261 gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg gtgtggagta
     4321 tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact gcccgctgac
     4381 accggaaaac taccatctgt tgaacgaagc cgccttcgat cagatgaaaa atggcgtgat
     4441 gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcggcaattg aagcgctgaa
     4501 aaatcagaaa attggttcgt tgggtatgga cgtctatgag aacgaacgcg atctgttctt
     4561 tgaagataaa tccaacgacg tgatccagga tgacgtattc cgtcgcctgt ctgcctgcca
     4621 caacgtgctg tttaccgggc accaggcatt cctgacagca gaagctctga ccagtatttc
     4681 tcagactacg ctgcaaaact taagcaatct ggaaaaaggt gaaacctgcc cgaacgaact
     4741 ggtttaatct tgcagctccc ctgcaatcca ggggagctga ttcagataat ccccaatgac
     4801 ctttcattct ctattcttaa aatagtcctg agtcagaaac tgtaattgag aaccacaatg
     4861 aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt aagtaatgac
     4921 aaaattgctg ttacgccaga acagctacag catcatcgct ttgtgctgga aagcgtaaac
     4981 ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga aaaaatgatg
     5041 atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa tggtgaactg
     5101 acagccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct taatgaactc
     5161 gataac
//
LOCUS       DAOFMX010000173         5154 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.175, whole genome shotgun sequence.
ACCESSION   DAOFMX010000173 DAOFMX010000000
VERSION     DAOFMX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.175"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            190..447
                     /locus_tag="QBA20_004132"
                     /pseudo
     CDS             190..447
                     /locus_tag="QBA20_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="QBA20_004133"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="QBA20_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDQ6294446.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="QBA20_004134"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="QBA20_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDQ6294447.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="QBA20_004135"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="QBA20_004135"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDQ6294448.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="QBA20_004136"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="QBA20_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="QBA20_004137"
     CDS             complement(3605..4420)
                     /locus_tag="QBA20_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6294449.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5154
                     /locus_tag="QBA20_004138"
     CDS             4517..>5154
                     /locus_tag="QBA20_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDQ6294450.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLV"
BASE COUNT         1375 a         1174 c         1227 g         1378 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggttggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc ttgt
//
LOCUS       DAOFMX010000174         5063 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.176, whole genome shotgun sequence.
ACCESSION   DAOFMX010000174 DAOFMX010000000
VERSION     DAOFMX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.176"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            224..408
                     /locus_tag="QBA20_004139"
                     /pseudo
     CDS             224..408
                     /locus_tag="QBA20_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            551..772
                     /locus_tag="QBA20_004140"
                     /pseudo
     CDS             551..772
                     /locus_tag="QBA20_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(793..855)
                     /locus_tag="QBA20_004141"
                     /pseudo
     CDS             complement(793..855)
                     /locus_tag="QBA20_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1305..2717
                     /gene="ospC1"
                     /locus_tag="QBA20_004142"
     CDS             1305..2717
                     /gene="ospC1"
                     /locus_tag="QBA20_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HDQ6294451.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2821..2970)
                     /locus_tag="QBA20_004143"
                     /pseudo
     CDS             complement(2821..2970)
                     /locus_tag="QBA20_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3046..4743
                     /gene="ospD3"
                     /locus_tag="QBA20_004144"
     CDS             3046..4743
                     /gene="ospD3"
                     /locus_tag="QBA20_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HDQ6294452.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4860..4979
                     /locus_tag="QBA20_004145"
                     /pseudo
     CDS             4860..4979
                     /locus_tag="QBA20_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
BASE COUNT         1722 a          939 c          963 g         1439 t
ORIGIN      
        1 ttcaatcaaa gcgcagaact tctgcgtctt cttcgtcaaa aaaatcatct tcgtgaagct
       61 tcacgacata gcggggaagt ttgcttcttt gagaggcggg tttacgttta cgggatttag
      121 ctgaacgggc catataacca cctgaaagac aatgacattg cctgttttta taacggtaat
      181 tgcagaccat gacaaaccgc agccgtcagg ctgcctactc gagtcacaag gctctttgtg
      241 tccgatatgc cggcagcgca ttacccatga aactggctgg aacgtacacc ataaggtgaa
      301 gaaagttatg ggggcggtga agaattatct aaccttgtct tgttacatcc aaattgccat
      361 cgacagttac acagttgtga agccggctct ctcaaaaagg gcttataaag gcttgagcgg
      421 tgtaccggga aaccggtatg caccgttctt aggggagggc agtccagcaa tgaactgtcc
      481 ttacccgaca aacatccaaa acgaacggaa cgtgctggaa tccgcatata acccgttgta
      541 aagtgaggaa atggacacct cacttgctca tgagaacgcc cgcctgcggg cactgttgca
      601 gacgcaacag gacactatcc accagatggc tgaatacaac cgcctgctct cacagcggat
      661 ggcggcttat gcttccgaaa tcaaccgact gaaggcgctg gttgcgaaac tgcagcgtat
      721 gcagttcggt aaaagctcag aaaaacttcg cgcaaaaacc gaacggcaga ttccattttc
      781 acgggcgatc tacgccacac aggctctggg ttgcagtgac tgctccatta aggcgatttt
      841 gaactcataa gggaagtaac aaaaaaactg taaaagtaca ataattatat atggccaaac
      901 taacctctat taaaaaacat cagtttttca cacttagttg agcttcatga aaaaatcatc
      961 gaggatttca ttgcagaata cacattccac taactggaag cactccataa gaggaatcag
     1021 agaaagtatc gtttttttat agtaaaattc attgctgttc aataaaattg atatagttat
     1081 taaattatga aaataatttc acaaaaaaga ttaacttgag ttcttaagca tcggatttta
     1141 gatattttga aatatctggc atcacataac atcaaaagta tataataccc ttatctaatt
     1201 acatgcagta tattgtatat atactcaaaa attgtaattc ataccctgct atatcttgta
     1261 gggaaagata ttaattaaaa ctgttttcat ataaggttca ttttatgaat atatcagaaa
     1321 cactgaactc agcaaatacc caatgcaata tagattctat ggataacaga ttacatacat
     1381 tgtttccaaa agtgacatca gtgcgaaacg ctgcacaaca aactatgcca gatgaaaaaa
     1441 atttaaaaga tagtgcaaat attattaaag atttctttag gaaaactata gcagcacaga
     1501 gttatagtag aatgttctct caaggctcta actttaaatc tttaaatata gcaattgatg
     1561 caccatcaga cgctaaagcc tcatttaagg ctattgagca ccttgacaga ttatcgaagc
     1621 attatatatc tgaaataagg gaaaaacttc atcctctttc tgcagaggaa ctcaatttgc
     1681 tttcgctaat tattaattct gatttaatct tcagacatca aagtaattct gatttgtctg
     1741 ataaaatttt aaacattaag tcattcaata aaattcagtc tgaaggaata tgcacaaaac
     1801 gaaacacata cgctgatgat ataaaaaaaa tagctaatca tgactttgtg ttttttggcg
     1861 ttgaaatctc taaccatcag aaaaaacacc ccctgaatac aaaacatcac actgttgatt
     1921 ttggtgcaaa tgcgtatatc attgatcatg actctccata tggatatatg acattaaccg
     1981 atcactttga taatgctatt ccacctgttt tttaccatga gcaccaatca tttttagata
     2041 aattttcaga ggttaataaa gaagttagtc gatacgtaca tggaagtaaa ggaattatag
     2101 atgtaccaat attcaatact aaagatatga agttagggct cggattatac ctgattgact
     2161 ttattagaaa aagtgaagac caaagcttca aggagttttg ctatggaaaa aatcttgccc
     2221 ctgtggatct ggatagaatc ataaactttg tttttcagcc agagtaccat atacctagga
     2281 tggtaagtac agaaaacttc aaaaaagtta agattagaga aatatcctta gaggaggctg
     2341 ttacagcatc taattacgaa gaaattaaca agcaggtcac taacaaaaaa attgctctcc
     2401 aggctctttt tctttcgatt actaatcaaa aagaggatgt cgccttatat atattatcta
     2461 attttgagat aactagacaa gatgttattt ccataaagca tgagttgtat gatattgagt
     2521 atctacttag cgctcataat tcaagctgta aagtacttga gtattttatc aataagggat
     2581 tggttgatgt aaacacaaag ttcaaaaaaa ctaatagtgg ggattgtatg ttggataacg
     2641 caataaaata tgagaatgca gaaatgataa aactattatt gaaatatggt gcaacatctg
     2701 acaataaata tatttaatca aaattgaata tcgtttagca aaaggcagta tttgcacaaa
     2761 atatcgatat attgtttatt gtcagtatgg ctcaatgtga taatagctgg aaagtttgat
     2821 gggtttcgcc ccgttgtagc ggtagtcgaa ctggaaggtc ttcaggcact gcttacagcg
     2881 atagagcagc ccccctgaac tggaatggcc gttccggata cccccctgag tttcagagta
     2941 acggtgacaa accacatcaa tctttgccat caatcatcca aagggcaaag agtacaacaa
     3001 cactaagtct gcgtcacaac ccatcaatga aaggaatata tacatatgcc atcagtaaat
     3061 ttaatcccat caaggaaaat atgtttgcaa aatatgataa ataaagacaa cgtctctgtt
     3121 gagacaatcc agtctctatt gcactcaaaa caattgccat atttttctga caagcggagt
     3181 tttttattaa atctaaattg ccaagttacc gatcactctg gaagacttat tgtctgtcga
     3241 catttagctt cctactggat agcacagttt aacaaaagta gtggtcacgt ggattatcat
     3301 cactttgctt ttccggatga aattaaaaat tatgtttcag tgagtgaaga agaaaaggct
     3361 attaatgtgc ctgctattat ttattttgtt gaaaacggtt catggggaga tattattttt
     3421 tatattttca atgaaatgat ttttcattcc gaaaaaagca gagcactaga aataagtaca
     3481 tcaaatcaca atatggcatt aggcttgaag attaaagaaa ctaaaaatgg gggggatttt
     3541 gtcattcagc tttatgatcc caaccataca gcaactcatt tacgagcaga gtttaacaaa
     3601 tttaacttag ctaaaataaa aaaactgact gtagataatt ttcttgatga aaaacatcag
     3661 aaatgttatg gtcttatatc cgacggtatg tctatatttg tggacagaca tactccaaca
     3721 agcatgtcct ccataatcag atggcctgat aatttacttc accccaaagt tatttatcac
     3781 gcgatgcgta tgggattgac tgagctaatc caaaaagtaa caagagtcgt acaactatct
     3841 gacctttcag acaatacgtt agaattactt ttggcagcca aaaatgacga tggtttgtca
     3901 ggattgcttt tagctttaca aaatgggcat tcagatacaa tcttagcata cggagaactc
     3961 ttggaaactt ctggacttaa ccttgataaa acggtagaac tactaactgc ggaaggaatg
     4021 ggaggacgaa tatcgggttt atcccaagca cttcaaaatg ggcatgcaga aactatcaaa
     4081 acatacggaa ggcttctcaa gaagagagca ataaatatcg aatacaataa gctgaaaaat
     4141 ttgctgaccg cttattatta tgatgaagta cacagacaga tacccggact aatgtttgct
     4201 cttcaaaatg gacatgcaga tgctatacgc gcatacggtg agctcattct tagcccccct
     4261 ctcctcaact cagaggatat tgtaaatttg ctggcctcaa ggagatatga caatgttccc
     4321 ggacttctgt tagcattgaa taatggacag gctgatgcaa tcttagctta tggtgatatc
     4381 ttgaatgagg caaaacttaa cttggataaa aaagcagagc tgttagaagc gaaagattct
     4441 aatggtttat ctggattgtt tgtagccttg cataatggat gtgtagaaac aattattgct
     4501 tatgggaaaa tacttcacac tgcagacctt actccacatc aggcatcaaa attactggca
     4561 gcagaaggcc caaatggggt atctggatta attatagctt ttcaaaatag gaattttgag
     4621 gcaataaaaa cttatatggg aataataaaa aatgaaaata ttacacctga agaaatagca
     4681 gaacacttgg acaaaaaaaa tggaagtgat tttctagaaa ttatgaagaa tataaaaagc
     4741 tgaatattat ctcgtggtga tacactgaat ttgaggtaac cggctcattt aaaccgtctg
     4801 gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga cactatcgta
     4861 tggaataccg gacctggatc actgaagctt tacgccttca tttcgaagaa catttaccac
     4921 gggttgtggc cggacgtcgc ctgggcgtac caaaatcaac tgtctgtggt atgttcgtgt
     4981 gaaccgcccc cggtatcccg ctcatgctgg cgaacccaga cacgcagtga accgccccgg
     5041 gtttcctgga gagtgtttta tct
//
LOCUS       DAOFMX010000175         5044 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.177, whole genome shotgun sequence.
ACCESSION   DAOFMX010000175 DAOFMX010000000
VERSION     DAOFMX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.177"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..297
                     /locus_tag="QBA20_004146"
     CDS             <1..297
                     /locus_tag="QBA20_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HDQ6294453.1"
                     /translation="LFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADN
                     QQVKLSMPELEELAAAMVQAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(498..1355)
                     /gene="sitD"
                     /locus_tag="QBA20_004147"
     CDS             complement(498..1355)
                     /gene="sitD"
                     /locus_tag="QBA20_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HDQ6294454.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="QBA20_004148"
     CDS             complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="QBA20_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HDQ6294455.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="QBA20_004149"
     CDS             complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="QBA20_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HDQ6294456.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3033..3947)
                     /gene="sitA"
                     /locus_tag="QBA20_004150"
     CDS             complement(3033..3947)
                     /gene="sitA"
                     /locus_tag="QBA20_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HDQ6294457.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4409..>5044)
                     /locus_tag="QBA20_004151"
     CDS             complement(4409..>5044)
                     /locus_tag="QBA20_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6294458.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1336 a         1230 c         1210 g         1268 t
ORIGIN      
        1 ctgttcacgt ttgaatggaa cggtcgtatc tggaatgctg gtcccgactc actgggccgc
       61 ctgtccccgg tagtcatgct ggcaaaatct gtcacagcac aaacacatat ggcgtggagc
      121 gatgccgata atcagcaggt gaaactgtcg atgccggaac tggaagaact ggcggcagca
      181 atggtgcagg cgcaggtcga tcgcaacgat gagatttatc gccgtcagcg ggagctgaag
      241 gaagagctga atagtctgaa ggatttgaat tcggttagga attttatcgt ggaataacag
      301 aagctgcggc acgtcgtatg caggaacgtg ccgcggttgg ctggtaaact ttcgatagtg
      361 cgagtattga atgatttcta ggcgttatcg attttacgta ttttttgcat gatagaattc
      421 gtacctcctc ccaacgacct tccatggctt tccggacttc tgtaggctgg cggacgatgc
      481 tgtcctcgga tgttgtgcta attgggtgat aacagatcct gtgtatgggc gtttcccttt
      541 gtgcgtgcgt tgatgctggt aacggtaaac gtcatgatga acacgaccgc gaataagacg
      601 acaatcgtcg gagctggtgc gctgtcgata aaaaaggaca gccagacacc agtcattgac
      661 actattaccg atatgccagt cgccagtagg agcgcaatat gaaagcgttg tgtcaggagt
      721 actgcaatgg cgccaggcgc aatgagcaga gaaatagaga gaatgatacc tactgctttc
      781 agcgtcgcca caatggtcag ggaaaccatg cacagcagcc cataatgcag ccatctcgta
      841 tgcaaaccgc ttacctgcgc ctgttgataa tcaaagctga acagcaaaaa atcccgccat
      901 tttacgctaa taacaagtgt aaccagcccg gcaataatca ccgtctggat tatatcgccg
      961 atggttatcc cgagcatgtc gccgaaaaga atatggtcaa gatgtacgtc tggtttaatt
     1021 gcaatataca agatcagacc tgcggcaaac atgccagaaa aaacaatccc cataactgta
     1081 tcttgcttga ttcggctgtt gtctttcagg tatcccgtcg ccaccgcgca gaatactccg
     1141 gcgacgaatg cccctgtagc cagaggtaac cccagaatcc aggtaagcac gatgccagga
     1201 aaaaccgcat gactcatcgc gtcacccatc agtgcccaac ctttcaggac cagaaagacc
     1261 gacagcagcg cacagggtat ggaaactacc agtgaaatca gcagtgcgtg actcataaag
     1321 gtgaactgca gtggctccag taataatgcc atcatgagcg atcctccagc gctttatgcg
     1381 cacgccgacg atttgccaaa agtccatggg tgggcgcaaa tacaaatgct aaaagaaaca
     1441 gcagtgtctg cgccaccacg atgattccac ctgtcgcgcc gtccagaaag taactgaccc
     1501 acgcgccgag gaaactggtt acactgccaa tagttacagc aattatgagc aggcggggaa
     1561 aacgatcggt taacaaccat gctgttgcac cgggagtcac caccagacag atcaccagaa
     1621 aggcaccaac cgtttgcagc gccgcaacgg tcgaaaccga cagcagggtg aagaagagga
     1681 tttttaatct gcccggatgt aatccaatag cacgggcgtg attctcgtcg aaaaaggtca
     1741 ccataagatc tttccatttg aaaaaaagca cgatgatcga gagaatgcca atgatagtga
     1801 gttgcaggat atccgccgga tcgattgcca gaatattgcc caggacgatg gtctgaatgt
     1861 tcacggatgt cgggttcaat gacaccataa acagccccag gccaaaaaag gacgagaaga
     1921 tcaggccgat gatggcatct tcttttaagc gggtgcgttg gttaagaaac agcatgctgc
     1981 ccgctgccag tccgccggaa aagaaggctc ccagagaaaa cggaagcccc agcatatatg
     2041 cccccgcaac acccgggaca atagagtgcg acagcgcatc gccaatcagc gaccagcctt
     2101 ttagcatcag atagcatgac aggaaagcgc agagaccgcc gaccattgcc gagacccaca
     2161 tcgcattgag catatattca tagctgaagg gttccagtag cacgttcatc ttgcttccct
     2221 ctgcaccgct gacggtcgat gtgccacaaa agggcgttca tcgtcagtaa taatgctttc
     2281 ttccgagccg ttaagtgtga catggcgcag tacgccgcta aaagccagct ccaggttctc
     2341 ggctgtaaaa gtggtgtctg tcggcccgct tgccaggacg gtgcctttga ccatgaccgt
     2401 ataatcgcaa aatgtcgtga ctgagccgag attgtgcgtt gagacaagca tcgttttgcc
     2461 ttccgcgcgt aactccctca acaggctgat gattttcgct tcggttttca catcaacgcc
     2521 ggtaaaaggt tcatccagca ggatcacatc accctgctgt gcaattgctc tcgccagaaa
     2581 gacgcgtttc ttttgtccac cggaaagctc gccgatttga cgatggcgaa aatccaccat
     2641 atcgacacgt tccagcgcat cggtgacaat ctgacgatcc ctctttttgg ctatacgcaa
     2701 catacccata tgcccatagc ggcccatcat caccacatct tccaccagta caggaaatga
     2761 ccagtcaacc tcttctgact gagggacgta ggcaaccagg tttttctgta gcgcctgtcg
     2821 cgtgggaata cccagaacag atatctttcc gctggtcaga cgcacaaatc ccataatcgc
     2881 tttaaacaac gtcgattttc cggaaccgtt taccccaacc agagcggcaa tagatccact
     2941 tggtactgtg aaggatgcat cgcgcagtgc tgtgtgcccg ttacgccagg tgacagtaac
     3001 atcattaacg acaatgcctg cagattgcat cattatttct cccttttccc ggctttaatt
     3061 ccctgtacca gcgtactggt agtaacctta agaaggtcga tatacgtagg taccgggccg
     3121 ttttctgtgc tcagggaatc gacatagagc acaccgccgt agtgtgcgcc ggtttcacgc
     3181 gcaacctgac gcgctggttt atcggaaatc gtactctcgc taaagactgc cgggatatga
     3241 tttttcttaa ctatatcaac aaccttacgt acctgctgcg gtgttccttg ttgatcggca
     3301 ttaatcggcc acagataaag ctcttttagc cccaaatcgc gtgcgagata agaaaaagcc
     3361 ccttcactgg tgaccatcca tcgctgattc tcagggagtt ccgtaatctg cttacgcagg
     3421 ggggcaaggg tttgggtaat cttggcttta taagtatcgg cattacgttg gtaggtttgt
     3481 gcatttgccg gatcgtattt tatcaacgca tcacgaatat tatcgacgta aatcagagca
     3541 ttatctggcg acatccatgc atggggatta ggtttgccct catagggtcc ttcggtgatc
     3601 cctactggcg tcacacccga agagacaatt acttctggaa ccccattgag atgctggtaa
     3661 aagcgttgga accacaattc cagattcata ccattggcga gaatcagttg tgccccctgc
     3721 gcacgtttaa tatcgccagg ggtaggctga tactcatgaa tttctgcacc aggcttggtt
     3781 atggatgaga cttctgcagc atctccagcc acgtttttgg ccatatccgc gatgatggta
     3841 aatgttgtaa tgaccttgaa tttttcagtt gcacttgcct gaaatgcgat cgagcaggtg
     3901 agggcgaggc accccaagag catggttact ttttttattg agtgcataat ttagtaccta
     3961 tagtgagcaa aattagaaca aacatagcaa cggctatgtt ttatagcata acctatacaa
     4021 aatgaaaata attatcatta acagctttga ggcctaaaac agggattagg ttgtttcctg
     4081 ctggcgacat gatattgagg aaaaaagtag agcagctatt aggctttgaa atgactttat
     4141 gaggaaaatt gttgatggca aaaatggggc aaaacgctgt ataaggggca aaaaaggggc
     4201 aaaaaagagt gggttatcgt agcttattgt tgtcgctgat aatgtttaac atattgaaaa
     4261 ataaataaaa tacttatgag tcagatagtt gtgatttttg cccttacttg ttcaggttgt
     4321 attgttcttt ctttgtaatt tgttgatttt cttgcattat ttcagttctc tggtactaaa
     4381 tggggcaaat tgggggcaaa ctttgcaact acgataaccg cgcattcaac atggctatct
     4441 gttcgtcgtt catgtcatca atccacatac cgtaaatttc atacaccatt tgcgcagttt
     4501 catgccccat ctggctggct ataaatgccg ggttcgctcc tgccgtcaac agccagcagg
     4561 caaaagtatg tcgcgtgtgg tacggattac ggcggcgaat accagcacgt tttactgctg
     4621 cattccatct tgtacccaaa ctgcttaccg agtaataagg tttctgtttt tcgttacaca
     4681 ccctgggcat gaaaacaaaa tgcagttttt gtttttcagt tctgccgtac tcccgatgat
     4741 aaaaagtgat ttcgcttttg cgatgatgcc cggtcagttt gtattgctcc ttcagtgctt
     4801 caagagccgg ctgcagtaat gttaccgtcc ggatcccggc atttgttttt gggggaccga
     4861 acatatcaag tatcgtcagg tttcttctga cattcacaat tcccttctcg aaatccacat
     4921 cctcccacgc cagagctgcc agttccccgt gacgaagccc ggagtaaacg gcaaatttcc
     4981 acaagttttg gctctgtcct ttttcacttt ccattaatgc attgaattct gttttagata
     5041 acgg
//
LOCUS       DAOFMX010000176         5005 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.178, whole genome shotgun sequence.
ACCESSION   DAOFMX010000176 DAOFMX010000000
VERSION     DAOFMX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.178"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..224
                     /locus_tag="QBA20_004152"
     CDS             <1..224
                     /locus_tag="QBA20_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDQ6294459.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="QBA20_004153"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="QBA20_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDQ6294460.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="QBA20_004154"
     CDS             518..1108
                     /locus_tag="QBA20_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HDQ6294461.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /gene="repA"
                     /locus_tag="QBA20_004155"
     CDS             1348..1608
                     /gene="repA"
                     /locus_tag="QBA20_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HDQ6294462.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="QBA20_004156"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="QBA20_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HDQ6294463.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="QBA20_004157"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="QBA20_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HDQ6294464.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3456..3647
                     /locus_tag="QBA20_004158"
     CDS             3456..3647
                     /locus_tag="QBA20_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294465.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
     gene            complement(3877..>5005)
                     /locus_tag="QBA20_004159"
     CDS             complement(join(3877..4256,4256..>5005))
                     /locus_tag="QBA20_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDQ6294466.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFL
                     KDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKK
                     TAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLSFRYLDHKTGETATETLTQRELVA
                     RLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLS
                     RDPFECVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT         1272 a         1320 c         1247 g         1166 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tataggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc tggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa aaaaacctgt tcactgtctg tcctgtggac agacagatat
     3121 gcacctccca ccgcaagcgg cgggccccaa ccgaagccgg tttagttaca acatgcaaac
     3181 ataaacccct aaaaaaccca agtccaacgc agaactgaaa ccacaacgcc cccctcataa
     3241 ctgaaaagcg ccgccgcccc cgcccaaagg gcgggaacag agtcgctttt aattatgaat
     3301 gttgtaacta catcatcatc gctgtcagtc ttctggctgg acgtatcgag tacacgctcg
     3361 taagcggccc tcacggcccg ctaacgcgga gatacgcccc gacttcgggt aaaaccctcg
     3421 tcgggaccac tccgaccgcg cacagaagct ctatcatggc tgaaaacggg tatggcttgg
     3481 cagggctggg gatgggtaag gtgaaatctg tcaatcagta ccggctgacg ccgggcttcg
     3541 gcggttttac tccggtatca catgtaacca cagcgtgccg ccttccatgc cgctggcgcg
     3601 gcatcagaat aatacaagca gcatttaatg catttgcaaa agtgtgagta atattaagtg
     3661 gtcgtttcag ggatatcgtt aagacttgag cgataggaat taaaacccca aaaagattaa
     3721 aaaaacacca caaaacggat gtttcttcaa caccactttt gctccatatg aacggaaccg
     3781 acgattaaac tggatggctc tgattgattc agggtatgaa tggcggtttt ttgctccgtt
     3841 tccctcaaaa tggacgcaac ttcccctctg cggctctcag ccgcaccacc gcatccgggc
     3901 cagcggctca tgcatcagga cctgctctgc cagacggtag ccccgcttca gccccgtaaa
     3961 acgcatctga ctcccgcaca gcacgcattc gaacgggtca cgactcagga actgtttcac
     4021 catttgtgca tagcacactt tcgccactgg ttccggttta tccatcccca gagcacggta
     4081 cacctgcggc agcttctctc cacacacccg gttggcaagg aacccgaagt acctcaccat
     4141 cttaaaaaac ttctccggga tgtgctgttt cagcctcgcg accagctcac gctgtgtcag
     4201 cgtttccgtc gccgtttctc ccgttttgtg gtccaggtaa cggaagctca ggctccccct
     4261 ccgttgtaat gtgccagccg ggaggccgct atcggcggct tcttcagata acgacccagg
     4321 tagcgcgccg tattcctccc gccggccgtt tttttcgaca tgtacacatg ccagtatttt
     4381 cctccggctt tcagcaccag gcttctcctc tgtgattccg tcgtgatatg tgacaacgac
     4441 tccggcatcg ccagcccctc tgaccacgct ttcagaagcc gttgccgcat attccacatc
     4501 caccgcgaac gcatcgcgtc tttcaggaag ctcagctttt tccactgacc atgcttattc
     4561 agacctccac aggttacaga cacatgtaca tgcggatgcc agttgagacg acggccatac
     4621 gtgtggatgg cgcagaagat accgggttcc tgcccccgtt tccgggcggc atacagcaga
     4681 ttctccaccg ccagacggca cacgtcattc agcagccacc ggttgctttc gaacaccggc
     4741 cactgcgtgt ccggcagggt gaagaccagg tgtacccagt cgcagtcagg aagacgattc
     4801 agctgtgtcg ctatccacag gtctgtggcc ttttttccgc aggacgggca ggcacggctg
     4861 ccgcatgagt tagtgacgta tctgacgtgg gggcactcag gtttatcgca gatgtattcc
     4921 ttcacgccca gtatccgcgt gccgcaggcc agcatttttg tgacagcctc aacctcgata
     4981 tcgcgcagac cgcccgcatc cagga
//
LOCUS       DAOFMX010000177         4997 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.179, whole genome shotgun sequence.
ACCESSION   DAOFMX010000177 DAOFMX010000000
VERSION     DAOFMX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.179"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..242)
                     /locus_tag="QBA20_004160"
     CDS             complement(<1..242)
                     /locus_tag="QBA20_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HDQ6294467.1"
                     /translation="MLPHFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAG"
     gene            complement(471..716)
                     /locus_tag="QBA20_004161"
                     /pseudo
     CDS             complement(471..716)
                     /locus_tag="QBA20_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558175.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            744..1091
                     /locus_tag="QBA20_004162"
                     /pseudo
     CDS             744..1091
                     /locus_tag="QBA20_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            1081..1875
                     /locus_tag="QBA20_004163"
     CDS             1081..1875
                     /locus_tag="QBA20_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDQ6294468.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            1897..3321
                     /gene="patD"
                     /locus_tag="QBA20_004164"
     CDS             1897..3321
                     /gene="patD"
                     /locus_tag="QBA20_004164"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDQ6294469.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            3531..4340
                     /locus_tag="QBA20_004165"
     CDS             3531..4340
                     /locus_tag="QBA20_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDQ6294470.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(4742..4837)
                     /gene="yncL"
                     /locus_tag="QBA20_004166"
     CDS             complement(4742..4837)
                     /gene="yncL"
                     /locus_tag="QBA20_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDQ6294471.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         1268 a         1091 c         1291 g         1347 t
ORIGIN      
        1 ccagccttca ccccgcagtg cgggcaggag cggcttttac accggaagca gacctttttt
       61 gtatggcaac agtccggtga tgaacaacac cactgcgtgt agcccatcag tgtggtcccg
      121 caggccatga tttttgtcac tgattcaatt accaccggac ggactgcccc ttccggttgt
      181 ttctccagcc agttaagcca gcggtttccc tgctgaaaaa tatcggcaaa atgggggagc
      241 atcagaaggg cggggcgact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca
      301 cgtataggaa attgaaaaac cagacgatac cgctgtccac gccttatgcc acgtgagctt
      361 ttctgctttt ccctccactc cggtgttttc tctctctgca tggtcgattc ttataccgca
      421 acaggctgtt ttttatactt gcaccgattc ccgggcacac ttagcctgtg tcaggccggc
      481 atatacctca gcagactgat atcccgcgcg tttttcttca gcccctccac attcagcccc
      541 gcaatggccc ggcggtatac catccggccg ccacacagca cgcattcgaa cgggtcacga
      601 ctcaggaact ttgagaggcg ggtttacgtt tacggggttt agctgaacgg gccatataac
      661 cacctgaaag acaatgacat tgcctgtttt tataacggta attgcagacc atgacaagcc
      721 gcagccgtca ggctgcctac tcgatgatcc tgcccgttca ggcggcgctt gagcgtttgc
      781 cgccgtcatt gttgcaggcg tcggctgatc tcggcgcacg tccacgacaa acctttcgct
      841 atgtggtgct gccgctggca atcccgggta ttgccgctgg ctctatcttt accttctcac
      901 tcacactggg cgattttatc gtcccgcagc tggttggtcc tccaggatat tttatcggca
      961 atatggttta ttcccagcag ggggcgattg gcaatatgcc gatggcggcg gcattcaccc
     1021 tggtgccgat tattctcatc gcactgtacc tggcgttcgt gaaacgtctg ggagcgttcg
     1081 atgcactctg aacgcgcacc gtttttcctc aaactggcgg cctggggcgg cgttgttttc
     1141 ctacattttc ccatcctgat aatcgccgcc tatgcgttta acactgaaga tgcggcgttt
     1201 agttttccac cgcagggcct gacgctgcgc tggtttagcg tggcagcaca gcgtagtgat
     1261 attcttgatg ccgtgacgct gtcacttaaa gtggcggcgc tggcgacatt aattgcgctg
     1321 gtgttaggga cgctggcagc tgccgcgctg tggagacgag actttttcgg caaaaacgcc
     1381 atttcgctgt tattgctgct gcccattgcg ctgccgggca ttgtcactgg tctggcgcta
     1441 ttaaccgcct ttaaaaccat caatctggag ccgggatttt tcaccatcgt ggtcggtcat
     1501 gcgacttttt gcgtagtggt ggtgtttaac aatgtcatcg cccgttttcg ccgcacctcg
     1561 tggagtctgg ttgaggcgtc aatggatctt ggggccaatg gctggcaaac cttccgctac
     1621 gtagtgttgc cgaatctcag ttcggcgtta ctggcaggag gaatgctggc gtttgccttg
     1681 tcgttcgatg aaatcatcgt tacgaccttt acggcaggtc atgaacgaac gttaccgttg
     1741 tggttgctca atcagcttgg gcgaccgcgt gatgtaccgg taaccaacgt ggtggcactg
     1801 ctggttatgt tggtaacaac cttgccgatc ctgggggcct ggtggctaac ccgcgaaggc
     1861 gacaatggtc aataaccact gatacaggaa taagctatgc aacataagtt actgattaac
     1921 ggagaacggg ttagcggcga aggggaaaaa cagcctgtct ataatccggc aacgggggac
     1981 gttttactgg agattgccga ggtatccgca gagcaggtcg atgctgctgt gcgtgcggca
     2041 gatgcagcat ttgctgaatg ggggcaaacc acaccgaaag cgcgtgcgga atgcctgctg
     2101 aaactggcgg atgttatcga agaaaatggt caggtttttg ccgaactgga atcccgtaat
     2161 tgtggcaaac cgctgcatag tgcgttcaat gatgaaatcc cggcgattgt cgatgttttt
     2221 cgctttttcg cgggtgcggc gcgctgtctg aatggtctgg cggcaggtga atatcttgaa
     2281 ggtcatactt cgatgatccg tcgcgatccg ttgggggtcg tggcttctat cgcaccgtgg
     2341 aattatccgc tgatgatggc cgcgtggaaa cttgctccgg cgctggcggc agggaactgc
     2401 gtagtgctta aaccatcaga aattaccccg ctgaccgcgt tgaagttggc agagctggcg
     2461 aaagatatct tcccggcagg cgtgattaac atactgtttg gcagaggcaa aacggtgggt
     2521 gatccgctga ccggtcatcc caaagtgcgg atggtgtcgc tgacgggctc tatcgccacc
     2581 ggcgagcaca tcatcagcca taccgcgtcg tccattaagc gtactcatat ggaacttggt
     2641 ggcaaagcgc cagtgattgt ttttgatgat gcggatattg aagcagtggt cgaaggtgta
     2701 cgtacatttg gctattacaa tgctggacag gattgtactg cggcttgtcg gatctacgcg
     2761 caaaaaggca tttacgatac gctggtggaa aaactgggtg ctgcggtggc aacgttaaaa
     2821 tctggtgcgc cggatgacga gtctacggag cttggacctt taagctcgct ggcgcatctc
     2881 gaacgcgtca gcaaggcagt agaagaggcg aaagcgacag ggcacatcaa agtgatcact
     2941 ggcggtgaaa agcgcaaggg taatggctat tactatgcgc cgacgctgct ggctggcgca
     3001 ttacaggacg atgccatcgt gcaaaaagag gtatttggtc cagtagtgag tgttacgccc
     3061 ttcgacaacg aagaacaggt ggtgaactgg gcgaatgaca gccagtacgg acttgcatct
     3121 tcggtatgga cgaaagatgt gggcagggcg catcgcgtca gcgcacggct gcaatatggt
     3181 tgtacctggg tcaataccca tttcatgctg gtaagtgaaa tgccgcacgg tgggcagaaa
     3241 ctttctggtt acggcaagga tatgtcactt tatgggctgg aggattacac cgtcgtccgc
     3301 cacgtcatgg ttaaacatta agttagatgt tagtggctgt tataggacgg ccactatcgt
     3361 tttaggttct gagtttaatg gcggttaagt attagaatga ttacattaat aactggcgta
     3421 aaaattaggc taaatgtttg atggcgattt ttcatcctgt cacagttgat tagcataagg
     3481 ctttagagta tcgttattaa actaatactt tataagtaga agtattaaca atgaaagttc
     3541 aattgtacaa attcacggaa gataaaaata aaacccttac cttcagatgg acgaaaaaac
     3601 actttgagtt ttgtatggat aataaaatct ttttaaatca taaggggaag aagtcgtata
     3661 aagaaaggaa tttatttctt ttctcaaaag gtgacaagat aacaattgaa gataatgtta
     3721 ttgctgaaga atattcaaca atgcctgtga aaaatttctc cagtgtcggc gctttttcat
     3781 ttccgacctg ccatttttca gggaatatac gtataggtcg attttgtagt attgcatcaa
     3841 atgtaaaaat aatgggtgga aatcatcctt tgaatcgttt tactactcac atgatgactt
     3901 ataacggtga atttgataag tttgctatga gtgaatttga acgtagctgg acattgaaac
     3961 cattcattac caaacctgaa aatccgatta taggaaatga tgtctggata ggaaatgatg
     4021 ttgtattgaa aggtggcatc gcaataggtg atggtgctgt tattgctgcc aattccgttg
     4081 ttaccaaaga tgtgccccca tatgccatag ttgcaggagt tcctgcaaaa ataatcagat
     4141 ttaggtttga ttcaaatgtt attgatgaac tgttgagaat aaaatggtgg aattataact
     4201 actcagatct tccggataat aataagtgtg atgatataaa ttattttgtt gaagagatga
     4261 atcgattgat atcaaatgga aatattcaag agagggacta taaaaaattc aatctttctg
     4321 aggtattcag aggcctataa ttatctgttt tgcatgataa atgggcaaga taaccagggc
     4381 ggggtttgca atttcaatgg ggcagcgtca tcaaggaggt tgcaaacatc ctgccatgca
     4441 tatactttac gccatctttt tatatttacg ttattccttg aaagtataaa gcatttatag
     4501 actcccgcgc gtataaagta ttcgtgatag aaaaatgtga aaacaagatt ccgtttgaat
     4561 cagtaagctg tttgtataga cgacagtagg gggatgtttt ctactcattt caatgtagat
     4621 gccagcaaaa agaagtaaat attaaggata atgttgcaaa cttggagagt atgagaagat
     4681 cttgcgtact gtcagagagt tgatttctct gacagtacgt gctgcgtgca aaatcactac
     4741 atcaaataaa ccagccaaat ctcatagaga taagagtaaa caaaccaaca gcagcaaaga
     4801 catttatcag tactacagtt ctactggaaa cattcatttt tgccaccttt tatcattgcc
     4861 cctatgtgta tagtacgggt taagaaaatc cgcaataata ctcattctga agatggataa
     4921 gggcaagttg ctgtttgatg attttttctc aaactggtga aaaaacttga tgcacgtcaa
     4981 aaaatggcgc atatttg
//
LOCUS       DAOFMX010000178         4986 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.180, whole genome shotgun sequence.
ACCESSION   DAOFMX010000178 DAOFMX010000000
VERSION     DAOFMX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.180"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..528
                     /locus_tag="QBA20_004167"
     CDS             <1..528
                     /locus_tag="QBA20_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDQ6294472.1"
                     /translation="RTTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSIT
                     LLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSV
                     IFINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLK
                     HDAVLIEAMESRSLR"
     gene            725..2164
                     /gene="aldA"
                     /locus_tag="QBA20_004168"
     CDS             725..2164
                     /gene="aldA"
                     /locus_tag="QBA20_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDQ6294473.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2206..3207)
                     /gene="gap"
                     /locus_tag="QBA20_004169"
     CDS             complement(2206..3207)
                     /gene="gap"
                     /locus_tag="QBA20_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDQ6294474.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3396..3926
                     /gene="cybB"
                     /locus_tag="QBA20_004170"
     CDS             3396..3926
                     /gene="cybB"
                     /locus_tag="QBA20_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDQ6294475.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYLTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4171..4344
                     /locus_tag="QBA20_004171"
     CDS             4171..4344
                     /locus_tag="QBA20_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294476.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4416..4565)
                     /gene="hokB"
                     /locus_tag="QBA20_004172"
     CDS             complement(4416..4565)
                     /gene="hokB"
                     /locus_tag="QBA20_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDQ6294477.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4666..4806)
                     /locus_tag="QBA20_004173"
                     /pseudo
     CDS             complement(4666..4806)
                     /locus_tag="QBA20_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1288 a         1144 c         1233 g         1321 t
ORIGIN      
        1 cgcaccactg gcagagcaga agcgaccatc ctggtggata tcgctcatca gttctggcat
       61 attccgcatg aaaaaatctg gattgaagac cagtcaacaa actgcggtga aaacgcacgc
      121 tttagcatca cgctattgaa tcaggccgta gaacgagttc atacggctat cgttgtacag
      181 gaccccacca tgcagcggcg cacgatggca acgttccgcc gtatgaccgg ggacaatccc
      241 gatgcaccac gctggttaag ttatcccgga ttcgttcctc agttaggaaa taacgcagac
      301 agtgtaatct ttattaatca gttacaagga ttatggccgg ttgagcgtta tctctcacta
      361 ctcactggcg agctgccgcg tttacacgat gatagcgatg gctacggtcc ccgcgggcga
      421 gattttatcg ttcacgttga ttttccggca gaagtcatcc atgcatggca aacgctgaaa
      481 catgatgcgg tgctcatcga ggcgatggaa agtcgctcgt tacgttaaaa attgcccgtt
      541 tgtgaaccac ttgtttgcaa acgggcatga ctcctgactt ttatttctgc cttttattcc
      601 ttttacactt gtttttatga agccctccac agacttgtcc tttcccgatg ccgtctctct
      661 gatgattgat gttaattaac aatatattca ccgaaaacaa acatataaat cacaagagtc
      721 gcccatgtca gtacccgttc aacatcctat gtatatcgat ggacagtttg ttacctggcg
      781 tggagacgca tggattgatg tggtaaaccc tgctacagag gctgtcattt cccgcatacc
      841 cgatggtcag gccgaggatg cccgtaaggc aatcgatgca gcagaacgtg cacaaccaga
      901 atgggaagcg ttgcctgcta ttgaacgcgc cagttggttg tgcaaaatct ccgccgggat
      961 ccgcgaacgc gccagtgaaa tcagtgcgct gattgttgaa gaagggggca agatccagca
     1021 gctagctgaa gtcgaagtgg cttttactgc cgactatatc gattacatgg cggagtgggc
     1081 acggcgttac gagggcgaga ttattcaaag cgatcgtccg ggagaaaata ttcttctgtt
     1141 taaacgtgcg cttggtgtga ctaccggcat tctgccgtgg aactttccgt tcttcctcat
     1201 tgcccgcaaa atggctcccg ctcttttgac cggtaatacc atcgtcatta aacccagtga
     1261 atttacgcca aacaatgcga ttgcattcgc caaaatcgtc gatgaaatag gccttccgcg
     1321 cggcgtgttt aaccttgtac tggggcgtgg tgaaaccgtt gggcaagaac tggcgggtaa
     1381 cccaaaggtc gcaatggtca gtatgacagg cagcgtctct gcaggtgaga agatcatggc
     1441 gactgcggcg aaaaacatca ccaaagtgtg tctggaattg ggtggtaaag caccggctat
     1501 cgtaatggac gatgccgatc ttgaactggc agtcaaagcc atcgttgatt cacgcgtcat
     1561 taatagtggg caagtgtgta actgtgcaga acgtgtttat gtacagaaag gcatttatga
     1621 tcagttcgtc aatctgctgg gtgaagcgtt gcaggcggtt caatttggta accccgctga
     1681 acgcaacgac attgcgatgg ggccgttgat taacgccgcg gcgctggaaa gggttgagca
     1741 aaaagtggcg cgcgcagtag aagaaggggc gagagtggcg ttgggtggca aagcggtaga
     1801 ggggaaagga tattattatc cgccgacatt gctgctggat gttcgccagg aaatgtcgat
     1861 tatgcatgag gaaacctttg gcccggtgct gccagttgtc gcatttgaca cgctggaaga
     1921 tgctatctca atggctaatg acagtgatta cggcctgacc tcatcaatct atacccaaaa
     1981 tctgaacgtc gcgatgaaag ccattaaagg gctgaagttt ggtgaaactt acatcaaccg
     2041 tgaaaacttc gaagctatgc aaggtttcca cgccggatgg cgtaaatccg gtattggcgg
     2101 cgcagatggt aaacatggcc tgcatgaata tctgcagacc caggtggttt atttacagtc
     2161 ttaatgagtg aaagaggcgg aggttttttc ctccgcctgt gcgcgtcaga gtttagcgaa
     2221 tttttcgagg gtgcgaataa gctgcgtgac gaagccatat tcgttatcgt accaggcgac
     2281 cgttttcacc agttgtaaat cgcccacggc ggtaatttcc gtttgcgtgg catcaaacac
     2341 tgaaccgaaa tggctgccaa tgatatcgga agagactatt tcttcatcgg tataaccaaa
     2401 tgactcgtta ttggtggttg cttgtttaag tgcgttattc acctcttcgg cagtcacttt
     2461 ttttccgaga atggacacca gctcagtgac cgaacctgtt ttcaccggca cgcgttgcgc
     2521 atgacctttc agtttgccgc tcagttccgg gatcaccaga ccaatggctt ttgccgcccc
     2581 cgtagtgtgg ggaatgatat tttctgccgc tgcgcgtgaa gcacgtaaat ctttaccacg
     2641 cgggccatcc accagtgact gggtgccagt ataggcatga atggtcgtca tcgtgccgac
     2701 ttctatcccg aaactgtcat gcaaggcttt ggccatcggc gcaaggcagt tagtggtgca
     2761 tgacgccacg gaaacaatgg tgtcgttgcc atccagagtg tcgtcattaa cgttataaac
     2821 gatggttttc atttcaccgg caggagcgga aatcaacacc ttcttcgcac cagcatcaag
     2881 atgcgcctgc gatttatcgg cggaggtata aaagccagta cattcgacaa tgatttctgc
     2941 ccctttcgct ttccacggaa tatttttagc ctctttttcg gcgtaaaccg cgatactttt
     3001 cccatcaacg ataagtgaat cttccgtaaa atcaacgctc caggggaacg gaccatagtt
     3061 tgaatcatgt ttcagcagat aggcgagaat ttttggggaa gtaagatcat taatagcgac
     3121 aacgtctatg ttgcttttga cttcaagtaa acgacgcaac accagtcgac cgatacgacc
     3181 aaaaccgtta ataccaactt tactcatggt tttctcctgt caggaacgtt cggatgaaaa
     3241 ttgatccttt ccaggcttag accaggattg tgggatgggc aatctccatt cttacagtga
     3301 aacgtaacgt actgaaaacg ggtgaacaat atttaatgaa attttgagaa aagcccgtta
     3361 tgttaacgga aaattatgtt aaagcaggaa atgttatgga aaataaatac tcaaggttac
     3421 aaatcagcat tcactggctg gtctttttgc tggttatcgc agcgtattgc gcaatggagt
     3481 ttcgtggttt cttcccacgt agcgatcggc cgctcatcaa catgattcat gtttcctgtg
     3541 gcatctcaat tctcgtgctg atggtggttc gtcttttgtt aagactgaaa tacctaaccc
     3601 cgccgattat acctaagccg aaaccgatga tgacgggact ggcgcatttg ggacatttgg
     3661 tgatttatct cctgtttatt gcgctgccag tgattggttt ggtgatgatg tataaccggg
     3721 gcaacccgtg gtttgcgttt ggtttgacga tgccttacgc ttcagaggcc aatttcgaac
     3781 gggttgatag cttaaagtcg tggcatgaga cgctggcgaa tctgggatat tttgtcatcg
     3841 ggttgcacgc tgcggctgca ctggcacacc actatttctg gaaggacaac acacttctac
     3901 gcatgatgcc gcgtaaacgt tcctgaagga tatttaaaga aaacgcctgt actaaaaccg
     3961 acccgtggta caggcgaaga atacgggtct acatcggaag cgcctattat atttatttgt
     4021 atgacaaata aaaccataat ccttgcccat acgtccatct ggcttatttt taatcaattc
     4081 acccgatctt tgatctcatc aacggtatca aaataaacca gcgtataacg ttcaaatcgc
     4141 acataagatt cgataaacca tggaggttat atgaaaaaac tggcacttat tttgtttatg
     4201 ggaacgcttg tttcctttta tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg
     4261 gggatctcac actgtacggc aggcggcaaa tttgtctgta atgatggttc tattagtgca
     4321 tcgaaaaaaa catgcactaa ctgaagtgta aaaggggtgc catgagaaaa tgaatctgct
     4381 agtcagatgc gcggggaaaa tccccgcgct tgcccttacc tggacgtgca ggccatgagc
     4441 gcagcaacct ccttatcacc gtcccggaac cgcagttcgt agagcgtttg tcgggtcagg
     4501 agtgtgaatg ccagaatcgt aatgcagata atgagcagac acaccaccag agggttgcgc
     4561 ttcatagcct ttctccttgc cggatggcgg gtaagaggct aacaatctga attgcttggt
     4621 tcattcgttg gcctcggttt gtactgaccc caaaaagttg gacagttaaa cacgaggcat
     4681 ataggtctga ttccgatatt catttggagt cagacctttt aatttcaggc taattcttct
     4741 gctgttgtag tattcaatat atcccgtaac agcatccttc agttcgctta tattactgaa
     4801 ctcattagac tggccccctg aatctccaga caaccagtat cacttattta agtgatagtc
     4861 ttaatactag ttttttgagg tagcctgagt ttaacggaca ctccttcctg aaatagaatg
     4921 gcatcagaag gagctaataa tgagcagaaa aacccaacgt tactctaaag agttcaaagc
     4981 cgaagc
//
LOCUS       DAOFMX010000179         4974 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.181, whole genome shotgun sequence.
ACCESSION   DAOFMX010000179 DAOFMX010000000
VERSION     DAOFMX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.181"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="QBA20_004174"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="QBA20_004174"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDQ6294478.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="QBA20_004175"
     CDS             1020..1703
                     /locus_tag="QBA20_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDQ6294479.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="QBA20_004176"
     CDS             1700..2161
                     /locus_tag="QBA20_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDQ6294480.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="QBA20_004177"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="QBA20_004177"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDQ6294481.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="QBA20_004178"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="QBA20_004178"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDQ6294482.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="QBA20_004179"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="QBA20_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDQ6294483.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="QBA20_004180"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="QBA20_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDQ6294484.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1175 a         1304 c         1241 g         1254 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcggcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tacg
//
LOCUS       DAOFMX010000180         4863 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.182, whole genome shotgun sequence.
ACCESSION   DAOFMX010000180 DAOFMX010000000
VERSION     DAOFMX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.182"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..426
                     /locus_tag="QBA20_004181"
     CDS             <1..426
                     /locus_tag="QBA20_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6294485.1"
                     /translation="NPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPL
                     GVYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSL
                     GTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            760..3039
                     /gene="ydeP"
                     /locus_tag="QBA20_004182"
     CDS             760..3039
                     /gene="ydeP"
                     /locus_tag="QBA20_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDQ6294486.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3287..3490
                     /gene="safA"
                     /locus_tag="QBA20_004183"
     CDS             3287..3490
                     /gene="safA"
                     /locus_tag="QBA20_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDQ6294487.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3558..4319
                     /gene="ydeO"
                     /locus_tag="QBA20_004184"
     CDS             3558..4319
                     /gene="ydeO"
                     /locus_tag="QBA20_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDQ6294488.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4721..>4863
                     /locus_tag="QBA20_004185"
     CDS             4721..>4863
                     /locus_tag="QBA20_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDQ6294489.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT         1390 a         1038 c         1079 g         1356 t
ORIGIN      
        1 aatccgcgta attttacgtg gaatattatt tcaaataaca gtgttgttat gccaacaggc
       61 ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc cggactttcc tggaagcgca
      121 gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc tctcatttta tctttcgggt
      181 gccaccactg acagtagccg acaagtgttt gctaacacag cacctgatgc aacaaaagca
      241 tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac tggctacggg agaaaatgtc
      301 tccctgggga cagtaaataa aagcaaagta cctctgggtt taagcgcaac ctatggacaa
      361 acaggaaaca aggtttcagc cggaaccgtc cagtcagtca ttggtgtcac ttttatctat
      421 gaataattgt gattttgacc ttagtctatg cgctccattc acgaaaattg cgcggataat
      481 tattttgtga aggctattat cctacacctg taagaaaatc cgcgcacaat tgaagccaga
      541 gtcttatttt ttatattgaa aaatatgtta atttaaacac tatgagagat gatgcttaac
      601 ttatcgtatt taatctattg tttaacgata atagattaaa aatagaaaga gagtagtccc
      661 ttactcttat atatccatgt tggcgataat ccctttgtat gtactgtgca tcatcgctat
      721 tacaaatcct aataattcat ttccacacag gataagtaga tgaagaaaaa aattgaatcc
      781 taccagggtg ctgcaggtgg ttggggtgct gttaaatccg tagcgaatgc agtacgtaag
      841 cagatggata tacgccatga tgttattgcc atgtttgaca tgaataagcc agagggcttt
      901 gactgtccgg gttgtgcatg gccagatcct aagcacagtg cgtcattcga catttgtgaa
      961 aacggcgcaa aagcaatcgc ctgggaagtc acggataagc aggtaaatgc ctctttcttt
     1021 gctgagaata cggttcaatc attacttacc tggggagatc acgagcttga ggctgcgggg
     1081 cgtctcactc agcctttgaa atatgatgcc gtcagcgact gttacaagcc attaagctgg
     1141 caacaagctt tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc caatcaggtt
     1201 gaattctata cttcgggccg cacttccaat gaagctgcct ttctttatca gctttttgcc
     1261 cgtgaatacg ggagcaataa ctttcccgac tgctccaaca tgtgccatga accgacaagc
     1321 gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg tgttgctgga agactttgag
     1381 aagtgcgatt tagtcatttg cattgggcat aaccctggta caaaccaccc tcgcatgctg
     1441 acttcgttgc gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa tcctctacag
     1501 gaacgtggcc tggagcgatt taccgcaccg caaaacccgt ttgaaatgct gacgaactct
     1561 gagactcagt tggccagtgc ctactacaac gtgcgcattg gtggcgatat ggcgttgctc
     1621 aaggggatga tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc aggtcggccc
     1681 tcattgcttg atgacgaatt tattcaaacg cataccgtcg gctttgacga gctacgccgt
     1741 gacgttctca attccgagtg gaaagatatc gaacgtattt ctggactaag tcagacacaa
     1801 atcgccgaac tggctgacgc atatgccgct gccgaacgca ccattatctg ttacggaatg
     1861 gggatcactc agcacgaaca tggtacccag aacgtgcagc aactggtcaa tttgctgttg
     1921 atgaaaggta acattggcaa gcctggtgcg ggtatctgcc cactacgtgg tcactctaat
     1981 gtacagggcg accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt tctggctcgc
     2041 ctgggtgagc gctatggctt ccccccacct catgcacctg gacatgctgc aattgccagc
     2101 atgcaagcaa tatgtacggg gcaggctcga gcattgatct gcatgggggg caactttgca
     2161 ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga tttggcggta
     2221 cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag ctatattctg
     2281 ccggttctgg ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc ggtaaccgtt
     2341 gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc cggtgtaatg
     2401 ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc ccagagcgtg
     2461 gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat tgaagctgtg
     2521 ctgccagagt tcgccgacta taaccagcgc atccgtcatc ccggtggttt tcacctgata
     2581 aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt cattaccagc
     2641 aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat ggcgacagta
     2701 cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta tcgaggggta
     2761 ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg ccgtgtaaaa
     2821 aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag ctcacgccgc
     2881 atggatagat taaaagtggt catttaccct atggctgacc gctcactggt gacctatttt
     2941 ccagaatcga atcacatgct aacacttgat aaccacgatc cattaagtgg cattcctggc
     3001 tataaaagta ttccggttga attagaacca tcaaattaat gcctcttctc atttcttctg
     3061 ctgtcatccg cacagcagaa gaattcctca ttgactatta ttgcgcaatt tgctcacatg
     3121 gattaaatta aactacatac tataagatat aaacttctgc ctacagctgt aagaaactcc
     3181 gctcagtact gaagcaccag tcctatttct tcttttctcc agcctgttat attaagcata
     3241 ctgattaacg acttttaacg ttatccgcta aataaacata tttgaaatgc atgcgaccac
     3301 agtgaaaaac aaaatcacgc aaagagataa ctataaagaa atcatgtctg caattgtggt
     3361 tgtcttatta ctgacactta cgttgatagc cattttttcg gcaattgatc agctgagtat
     3421 ttcagaaatg ggtcgcattg caagagatct tacacatttc attatcaata gtttgcaaga
     3481 ctggaaataa acagcaaatt ataaatgtga aatgtttaaa aggtatcgat aaaactttat
     3541 tgttttaagg agataaaatg tcgctcgttt gttctgttat atttattcat catgctttca
     3601 acgctaacat tttagataaa gattacgcct tctccgacgg cgagatcctg atggtagata
     3661 acgctgttcg tacacatttt gaaccttatg agcggcattt taaagaaatc ggatttaatg
     3721 aaaataccat taaaaaatat ctacaatgca ctaacatcca gacagtgacg gtgcctgttc
     3781 ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt gcttaatgaa atgattgctt
     3841 atctcaactc ggaagaacgc aatcatcata atttttcaga acttttgctt ttttcttgcc
     3901 tgtctatttt tgccgcatgc aaaggtttca ttacactatt aactaacggt gtgctatccg
     3961 tttccgggaa agtgagaaat attgtcaaca tgaagctggc gcacccatgg aagctgaaag
     4021 atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta aagcaagagc
     4081 aaacgacatt ctcacagatt ctattagatg ccagaatgca gcacgcaaaa aatttgatac
     4141 gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt acatcttatt
     4201 ttatttatgc gttccgcaaa catttcggca acagtccgaa gagagtttct aaggagtacc
     4261 gttgtcaaag gcacacgggt atgaatacgg gcaacacgat gagtgcttta gctatttgat
     4321 tatttgctaa cgagtagtca accacgcacg ctacgtaaga attatatggg gcagccattc
     4381 cctgccccgc gttgttttta ggcgatatat ttattgaaat aaataagtga catccatcac
     4441 atatttatgc acttgcataa cctgttgcat gattatttat gatctcaatt cggcattttg
     4501 tcagtaaaat gcaataattt attaaatatc aataaattag ttgtttatcg gcgagaaatt
     4561 acttaataga acagaaagta atgtcaacgc tttatggact gttatttccc tttttttagc
     4621 taaatctgct atctctttat gtgactaact tcacttacat ccacttattt ctcttcgtaa
     4681 aattacttcg gaattaagta caataagaag aggaacattt atgaagtctg cattaaagaa
     4741 aagtgtcgta agtacctcga tatctttgat actggcatct ggtatggctg catttgctgc
     4801 tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca aacgttgctt tctcagactt
     4861 tac
//
LOCUS       DAOFMX010000181         4550 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.183, whole genome shotgun sequence.
ACCESSION   DAOFMX010000181 DAOFMX010000000
VERSION     DAOFMX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.183"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..1224
                     /locus_tag="QBA20_004186"
     CDS             <1..1224
                     /locus_tag="QBA20_004186"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDQ6294490.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1898
                     /locus_tag="QBA20_004187"
     CDS             1224..1898
                     /locus_tag="QBA20_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6294491.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2426..3187
                     /locus_tag="QBA20_004188"
     CDS             2426..3187
                     /locus_tag="QBA20_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDQ6294492.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3231..4310
                     /locus_tag="QBA20_004189"
     CDS             3231..4310
                     /locus_tag="QBA20_004189"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ6294493.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4307..>4550
                     /locus_tag="QBA20_004190"
     CDS             4307..>4550
                     /locus_tag="QBA20_004190"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDQ6294494.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1249 a          864 c         1119 g         1318 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgtaggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgctta caaatatagg tgattttttt
     2221 attgtaggct tttgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgcagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt acgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttatat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggtgt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagtatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa aacgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat
//
LOCUS       DAOFMX010000182         4351 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.184, whole genome shotgun sequence.
ACCESSION   DAOFMX010000182 DAOFMX010000000
VERSION     DAOFMX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.184"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..121
                     /locus_tag="QBA20_004191"
     CDS             <1..121
                     /locus_tag="QBA20_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HDQ6294495.1"
                     /translation="AQTELDYFIPSRVLVGLGAVCFTLFSIVSILEAGSAKKN"
     gene            complement(208..684)
                     /locus_tag="QBA20_004192"
     CDS             complement(208..684)
                     /locus_tag="QBA20_004192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294496.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(677..1441)
                     /locus_tag="QBA20_004193"
     CDS             complement(677..1441)
                     /locus_tag="QBA20_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDQ6294497.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            1687..2448
                     /locus_tag="QBA20_004194"
     CDS             1687..2448
                     /locus_tag="QBA20_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDQ6294498.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(2555..3055)
                     /locus_tag="QBA20_004195"
     CDS             complement(2555..3055)
                     /locus_tag="QBA20_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294499.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(3206..>4351)
                     /locus_tag="QBA20_004196"
     CDS             complement(3206..>4351)
                     /locus_tag="QBA20_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ6294500.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDIENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT         1340 a          935 c          755 g         1321 t
ORIGIN      
        1 cgcacaaact gagctggact actttattcc atctcgggtt ctggttggat taggcgcagt
       61 atgttttaca ttgttctcta ttgtttcaat attagaggcc ggttctgcga aaaaaaatta
      121 aaatttacat aatcccagcg ttattcatat cttaacgctg gttttttacg aacttagtga
      181 aataatgtag aatataaaat tataatttta tattggagca catgtgttaa tgatcaaccc
      241 cgggatacta aaaatataga cattattaaa cttgcttagc ctcgcattgc ctttacctgt
      301 taaagatgca tcgagaaaat tgagtctgaa gttctcatcc tttatctgat tttccagtcc
      361 cgaaactttg gccttagcat tacctgagat ccgtccgtta ctatctattt tgtaggtaaa
      421 aaaatacgaa tgggaaaatt tcctcggtgt atcttcatca gttaagccaa caatcgtaaa
      481 actaccgcta ccatcttttt ttaaaatgac tgaaaggtta gcagagtagt ataaagaatg
      541 atctgtttta gcatttcttg ttaacgtcga cgtacattca agagacgttg tgctaccatc
      601 acgagccttc cagagagcaa aaaaacacac ctgtagcaat gtgaaaacaa gtattaatat
      661 aaaatacatt ttcttattag tcatataata gcacctctga acaaattgca taagtttcgt
      721 cattagagga ttttatacat cttgataaat accgctgccc ttccccaagc aaataatgca
      781 tactatttac atcatatata aaatttgcac ctggcacaca gggtaaatgc tttttcgaaa
      841 tttcagcggc ctctatttga gatttagcga ggccaatctc gttaagattc tctgaaagat
      901 ataaatcaca ggtgtcaatc ttacctgatt taaccaatgg ataatccgtt atactgtagg
      961 tatctttaaa agccacggca aacatttcaa taataacaat tactaccaca agcgaaatca
     1021 aaatgcgaaa aagaggaata ctccttttgc gctctacttt ttcaacaaca tcttcttgta
     1081 aatgagaatc atcataagat tgtttactta ttgttatgtt ttcactgatg taaaagccta
     1141 ctttgggtat cgtaagaatc acattttctt caacaccaat atttttaaaa cttttgcgta
     1201 acagggatat atattggttc aatgtgttgt ttgacagatc aaatgaaaat ctctccaaaa
     1261 cgctatcaat gattgagttc ctgtcaaccg ctccaccatt actttccagt aacactgcca
     1321 aaatacaccc cgccgtagct ggcaaattta tctcatcctg ggtatttaca ttcgagagcg
     1381 agcatttgtt cggatcaaaa ataatcgcgt cattgataat gaaaaaacat ctccgctcca
     1441 tgcaggcaac aacacaaaat aagattttgt cattttagcc tattcctggt ggaactgaca
     1501 caagaaacta aaatacaaat ataaccctga aaaagtcata taaaacttat atccacagaa
     1561 taaaaaatca aacatgtcag ttttatcaat ataaagtatc gcaaggcgaa aagtgatgca
     1621 agaatactcc taagtaaatg aaagcgcacc acatgaaatt atcaacacat gtaatttcat
     1681 cattaggtgc tatcgaaaat aactcaggag ctacttaaga tgaataatgt cttactcatg
     1741 agacccgatg actcagaatt tatgatacct gatgactggt cagggctaaa cgcaaaaggc
     1801 attacatacg aacaagataa tagagtgaca gagtcttttc tgccttttaa atctttttat
     1861 tgttcaccgg tgtgcacgac agttgaactc atctcacaaa ataattgttt tgctatgggt
     1921 atccgctacc ttctggaaaa gaaaagagac ttaaggctaa atcactttaa agccttctct
     1981 cataaatgcg agacaaaacc tgacgtgtta attatgaccg ggtgcaccgt cgctgatact
     2041 tatagtatat tctcacgcat tgttgaagaa aatataatga aaacgatgac acctatcgtc
     2101 atcattactg ggcatcactc agaagatctt tgttctctgg catcgaaact gggaggagtt
     2161 aaaataatat caccgacaga aaagattgca aaaatagaaa acgctatcaa tgatgctttt
     2221 aacagcaagc ctaaaaaaat aagattttta acagatatac tctctcctca acaaattgag
     2281 accatgaaaa tggttgcgca gggtagatcc gttaatgaga tttcagccgc aatgaatatt
     2341 agcatcaaga ccgtgtatgc acataaagtt cgtgctatca ataagctggg aattaagaaa
     2401 aaagtttatg aagcgctttt ttataaagcg ctaattcagt tcaactgatg taaaaatcac
     2461 cctcgcttaa taaataatat attctatgtc tttttttata agttaaaaaa ggcgggatat
     2521 caaaccccgc catgaaatta actaatcatc tacattattc tgcgtagata taagcctggg
     2581 tacggatggt gtaagagtct ggctcaacaa cctgttcttt catatgggaa acgacgcgat
     2641 aggcaccacc tgttccagta tttttcagca tcatataacg cttaccgcca atctcttcgg
     2701 aggcagctga aggaggatcc tgctcaagcg ttgtcaggga aactttcagt ttatggtccg
     2761 ggttattgtc accagaaatt actgcatccg ctgaagtact gctctccgca taaatatcat
     2821 ccgggaaagt tacagcatag tcgtggctct gcccatcaga tgcgccaaca atacctgttg
     2881 ccaaaggcgt ccatgcagtt aacgtaccgc tatatacccc tttcactggt tgaatggtat
     2941 tattaacaac gataggcttg gtcaaagtaa gattagctga cgcttcagta ccctggctta
     3001 ctaaagttcc cgcctgagaa gcaaaagctg tccccgccag catgattgct aacaatgttt
     3061 tcttcattat attttctcca tgattttcat ttacactgca aaatgcagtt tctttctttg
     3121 catttacgtg ctatcgcgaa aatgcttcca ccaaataatg gaaacatctt cacaaaaata
     3181 aattatgagt ttcttaattt agaaactata aacatgtcgc cgctatctgg tataaccctg
     3241 atgcactttt cttcgcagga agttggtatt ttgcgataca ggattgatct ttagaataac
     3301 cccatttcac attaaactcg ccttccgcac tcaagccaga aacataagca ataccatctt
     3361 cacctacaat gccggccata ttttcaatgt ctttgctata actaatcaca gcgccgaagg
     3421 ggacgggttt accctctttc tgttttaagg ttactaatcc tttatccccg ctcttcactg
     3481 ccaatgtcgc aggcacaacg gctccagctg tcggtacaac caattgacta gtttgtggta
     3541 agtcgtaatt cttacctatc gttttttcat ccagaataac atcgtctctt cggtatggct
     3601 gcactggcag tattgcatac ccacgaaaat cagtactcag tcccggccag ccattaacgt
     3661 taacccccgt tgctccgggt gcctctataa gggctaatgt cccatccaca ttttgaccaa
     3721 atgtaagtcc atcccttgta ataagtatcc caccgcttaa atttgcacct agctgtttca
     3781 tgttttcgct ggagctgtaa tggctactaa actgaccata tgatccattt ttgccaactt
     3841 gcacaatgtt catcgtgctc ttcggttgtg actgatgagc gttagttata tttgtcgcta
     3901 catcccacca tacaccacca ctattctgac ctgataatga aacctgatag ttattaccct
     3961 gctctcccga acgactcatc tgtgaagacg cataaatttg attttcgtca cccagcgata
     4021 atggcactga aatacccacg cttaatttcc gatctacttt ccatttatgc gatgtcagca
     4081 agtcgttttt tgcccagtta atggagaatg ttatgcctct ccataccgta ctataactca
     4141 ggctggtaga ccgcgtgttt cctgtgcccc gggaagaatt atacctcgtg gtattgaagt
     4201 tcaaattccc gatattacca aggggctggg ataaggatat cgtagagcca tcgtgactat
     4261 tcttatctct ttttctgttc cgttcatcct gtgcacttac tgcttcagtc agtgtggcat
     4321 aacctgatgc ataacggttc gtggttatag a
//
LOCUS       DAOFMX010000183         4322 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.185, whole genome shotgun sequence.
ACCESSION   DAOFMX010000183 DAOFMX010000000
VERSION     DAOFMX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.185"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..156)
                     /locus_tag="QBA20_004197"
     CDS             complement(<1..156)
                     /locus_tag="QBA20_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HDQ6294501.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTY"
     gene            complement(306..467)
                     /locus_tag="QBA20_004198"
     CDS             complement(306..467)
                     /locus_tag="QBA20_004198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294502.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(464..652)
                     /locus_tag="QBA20_004199"
     CDS             complement(464..652)
                     /locus_tag="QBA20_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HDQ6294503.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            912..1247
                     /gene="iraM"
                     /locus_tag="QBA20_004200"
     CDS             912..1247
                     /gene="iraM"
                     /locus_tag="QBA20_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HDQ6294504.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1528..1636)
                     /locus_tag="QBA20_004201"
                     /pseudo
     CDS             complement(1528..1636)
                     /locus_tag="QBA20_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2159..2235)
                     /locus_tag="QBA20_004202"
     tRNA            complement(2159..2235)
                     /locus_tag="QBA20_004202"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2199..2201),aa:Arg,seq:tct)
     gene            complement(2337..2412)
                     /locus_tag="QBA20_004203"
     tRNA            complement(2337..2412)
                     /locus_tag="QBA20_004203"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2377..2379),aa:Ile,seq:cat)
     gene            complement(2557..3378)
                     /locus_tag="QBA20_004204"
     CDS             complement(2557..3378)
                     /locus_tag="QBA20_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDQ6294505.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3393..3749)
                     /locus_tag="QBA20_004205"
     CDS             complement(3393..3749)
                     /locus_tag="QBA20_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDQ6294506.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3762..>4322)
                     /locus_tag="QBA20_004206"
     CDS             complement(3762..>4322)
                     /locus_tag="QBA20_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDQ6294507.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNE
                     KYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLA
                     FEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1187 a         1178 c          855 g         1102 t
ORIGIN      
        1 atatgtcagc agcccaaaca gcagactccc cagcacgcct attgccgccc actgagacgg
       61 ggaaaccctg tccagcaact gcaggaacca gtagcccgtt cccaccgctg acgtggtgta
      121 tgacacacct gttgtgattt tttccatctg gtccataccc cgtctcccgt tatccggaag
      181 ctgacaacaa taaaaaagcc accagttaac tactgatggc tctgataact catgcaagcg
      241 tctcagacga cccactgaca ctaccggtga gtttaacgat accttccatt tgactggctc
      301 actttttatg atgatgccgg tgcatttatc tccagcacca gactttctat ctcaacgcca
      361 tacgttgcat ttttggtaat atccgtcagc gtcagtgcat ttagtcccac tgccagactg
      421 tcttttatgg cctggaatgc cgggccagta cgatgacgta gtatcactcc ggctcagttg
      481 caccactgac caccacatca ccttctgctg caatcgcctg catcagggta taaggggtta
      541 tggccaccgg actaccaaac ggctgccagc cctctttcag tttatgtgtc agcttttccg
      601 caagatctga cggcggcgcc gccctgacaa catcatagtg tttaaatgcc atggttcttt
      661 ccaacatctg aaaaataatt ctttaaaata cctgacatgt aatacagaaa aaacacaaaa
      721 ccatacctta aataaaaacc tgattatcaa gcagatatgc atggataaac tacaagacga
      781 gatataaacc accctgcatt taaataaaca ataaacaaca tcagaaaaat aattctgctc
      841 tatggtttaa ttcaaaaata tcatttatac ttttcagaac atcaccagca aggcataaac
      901 aaggaaacta aatgaagtgg attgtgattg atacagttat ccagccatca tgcggaatat
      961 ctttttcagt catatggagt aaaataaaat taataatctg gtatcaatcg gatgctttct
     1021 tacctcctga aagtatattt acactgactc acacaggcat catgctcaat aacaaagtgc
     1081 tacctgtaac catttacaac gtagtaccat tcaataaaac attctggaat ttaatcaaaa
     1141 acagccagga atgccctaca aatacagata acgtattgaa tgaatgcttt aataaccgtt
     1201 gcactctgca aatatgtcct tatggactaa aacaacaaag tccataagga gtttactcac
     1261 atctgacaaa atcaatataa acagcccctc cggagagggg ctggagagtg gcgctatgtg
     1321 ccattccatg gtgccgggtg cctcccggtg aattcagtac cagcacctga atccgcgatt
     1381 atcccatata cctactcgct gattgcccct ccgcacaggg ggattcacca tgccagtttc
     1441 ttttaacaaa ctccccgcaa accagacaac agtcaaccgc ctgaattgtg aagtatttaa
     1501 aaatttctcc cgctaactga tacccggcta acagtctggc gttttctttt tcagcaacgg
     1561 gaaagcaaca accaccacac ccgccaccag cacaccgtca gccggcactg acattatccg
     1621 gctgctgcaa tgccattaac aaaaacagta agcaatcact ttttaccgta acaggtgata
     1681 atccagatat gtatctaccc cagatgagta atccgaagtt catccatacc acaggtcctg
     1741 gctattctgt tgtactcctg aacaagagca aataattctg aattagcaac catgaactca
     1801 tcgcaaaccc tctgtatagc atcactattc agaataataa cgtctcttcc cgaaagacga
     1861 tcaggagtac agaacaaaac tgtcaaacgg ctgaaggcct ttgctcgtgc tgcattgact
     1921 atatcaatac gctgcctaag gatgaaacac cccgacgcct catcaatatt cactctaccc
     1981 acaccatatg aatgataaat acttaatgct gaaaaaacca ttagaccgta taacaaatac
     2041 tcaatcaata cttaacagaa cttttatttt tgacaaatat ataatatttt caacaatatc
     2101 ctgagccagg tatatttcag tataaggctc tgccggaagg aatctggaag aatgaatatg
     2161 gcgcgctgta ctggattcga accagtgacc gattgcttag aaggcaattg ctctgtccgg
     2221 ctgagctaac aacgctgaat accgataatg gaccgccatc ggggacccgc cccccgcacc
     2281 aacaaccctg ttatcgtgtc gtctgctctt cctgataagc taatggcggt ttgtgatggt
     2341 ggcccttgct ggatttgaac cagcgacctg gcgattatga gtcgctcgct ctcaccactg
     2401 agctaaaggg ccgggagcag aataataacg gtccgtaatt aatttcgcaa taaaaaaacc
     2461 cgctcagcgg cgggttgtag aaactcttct aacgccaggc ataaaacgcc catcattatg
     2521 acgaatttac cacagattcc ggaaaaatca accttgttac ctagttacct tttttaactg
     2581 ccgctcagcc catgcttctt caatatcaaa ccgggtcacc agcgcatcat aaaatttctt
     2641 aactgttttt tcccatgacg cgcgtgttat ctggtttgtc acctcgcata tagcattaaa
     2701 tgcctccgtt gatggtagtc tttcatagcc acgaccacca caacgctggc agtctctgat
     2761 aacaggcata ccacgtttta ccgactcttc acgatgaatg gcgacaccac gcccacggca
     2821 atccttacag gcggtggaaa cctcaccctt tccgccacac tccggacagg caacttttac
     2881 cacctccctg acttttttcc attcttccca gtaagacgga tacacacctt tcgtacactt
     2941 tgcccatacc ggcggcttac catccggata ctggatcttg tttgtaaaaa ctatgctttc
     3001 aataaatttt tccccatagc aacaagggca ctgcttttta ctcgctgcgc tgcgggcata
     3061 atcctcaaaa gcgtacgaag ccataatgcg catcactacc ggttttattt ctgccggaag
     3121 ttttctcaac gccgccacac gatcgcaccg actgagtgca taatctgcca gtaattctgt
     3181 tgcccgcgcc ctgtcattca tactgatgcc cattttcccc aggaacgcag aaaaacccat
     3241 ctcagcccga ttctgtgtca tgccctgcgc ggccatcaca tcagtgatac tcagcgcatc
     3301 ttttgacgtt gaggccgatg catcggtcag gccaggggat tttggggagt agtatttcgg
     3361 taaatcttcc agtttcattt tttgacctgc tcttaatgca ttatggggta aatcttcacc
     3421 cccagacgtc caccagatac tggctgacca cgaacgatat tgatttcatc aaactgctca
     3481 tcgtccatta acactcccgc atgcgtcagc gcatccagcg gtgctttcag gatattgtcc
     3541 aggtcgcgac gacgcttatc cggtggctct gcaatcacct ttatcgccag ccttccggac
     3601 aggcttaatt tcagccgctg ctggcgaaca ataagcgcca cagcccggcg ataacgcttt
     3661 ccctcctccg agataaaata tgtgctgcca cggcgtcgcc agtaagtgtt caccgtcggc
     3721 gggtaaggta aaaccaaatc tatgagcatc agtcacctct tttacccaag cacgccagtt
     3781 gcaaaggcgt gatcaagaaa acgaaaaatt aaatcaacct gagaaccatg cttttcttcg
     3841 aacgccagag gatccgcatg aagctcgttg tgatgctccc gacacagcgg tagcgtgaaa
     3901 atatcgtgag atttggtccc cattccgccc tgaccatgac caatcaggtg atggggatcg
     3961 tcggctggct taccacaaca cgcacacggc tgtgtcttca cccagcgttt gtatttctcg
     4021 ttaacccagc ggcgacgttt aggtcgtttc atgaaagatt ccggagactc aggatcaacg
     4081 gcaatgctga ccaccgtctt ttcctgttgt gggttctgtt gctggtgggc gtgaggcagc
     4141 ggcgcaagat tttttgtgcg ctgtttcagt atgctggtgg cggtctgctc tcccggtacg
     4201 atgtcgcttt cacggtacat tgagcggatt ttttccgcac gcaaccccag cgaacgacgt
     4261 aataccgctt ccggtagcgc gtccgccacc tgattgcgga ccgcccacca ggataattca
     4321 gc
//
LOCUS       DAOFMX010000184         4209 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.186, whole genome shotgun sequence.
ACCESSION   DAOFMX010000184 DAOFMX010000000
VERSION     DAOFMX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.186"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="QBA20_004207"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="QBA20_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDQ6294508.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="QBA20_004208"
     tRNA            complement(324..399)
                     /locus_tag="QBA20_004208"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="QBA20_004209"
     tRNA            complement(406..480)
                     /locus_tag="QBA20_004209"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="QBA20_004210"
     tRNA            complement(597..681)
                     /locus_tag="QBA20_004210"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="QBA20_004211"
     tRNA            complement(690..765)
                     /locus_tag="QBA20_004211"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="QBA20_004212"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="QBA20_004212"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDQ6294509.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="QBA20_004213"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="QBA20_004213"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDQ6294510.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="QBA20_004214"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="QBA20_004214"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDQ6294511.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1090 a         1047 c          936 g         1136 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgc gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 gatgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAOFMX010000185         4200 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.187, whole genome shotgun sequence.
ACCESSION   DAOFMX010000185 DAOFMX010000000
VERSION     DAOFMX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.187"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            86..754
                     /locus_tag="QBA20_004215"
     CDS             86..754
                     /locus_tag="QBA20_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294512.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(997..1692)
                     /locus_tag="QBA20_004216"
     CDS             complement(997..1692)
                     /locus_tag="QBA20_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDQ6294513.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1685..3112)
                     /locus_tag="QBA20_004217"
     CDS             complement(1685..3112)
                     /locus_tag="QBA20_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDQ6294514.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3123..3842)
                     /locus_tag="QBA20_004218"
     CDS             complement(3123..3842)
                     /locus_tag="QBA20_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDQ6294515.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1127 t
ORIGIN      
        1 ttttgctgat atttaacccg aacattcctg atacagacta ttaatacaac attatctgcg
       61 acagatttta ttttctggat atattatgag tgaacaaatc aaacaggata tcgatctgat
      121 tgagatttta tttcatctga agaaaaagat tcgtgttatc ctttttatta tggctatctg
      181 tatggcaatg gtgctgttgt ttctgtatat caataaagac aatacaaaag tgatttacag
      241 ccttaaaata aaccagacaa cgccaggtat acttgttagc tgtgatagca ataataattt
      301 tgcctgtcag accacaatga ctgaagatgt tattcagcga attactacat tttttcagac
      361 cagcccagat atcaagaaca gagaaataag gctggaatgg tcaggagata agagagattt
      421 accaactgct gaagaggaaa tatctcgcgt gcaggcgtct attatcaaat ggtatgcgtc
      481 agaataccat aatggcaggc aagttctcga tgagatacaa acgccttcag caattaacag
      541 tgagctttat acaaaaatga tatacctgac caggaactgg tcattgtatc cgaacggtga
      601 tggctgtgta actataagct caccagaaat aaaaaataaa taccctgctg ccatttgcct
      661 ggctctggga ttttttctaa gcattgtaat ttctgtaatg ttttgccttg tcaaaaaaat
      721 ggtagatgaa taccaacaaa actctgggca gtaataaaca gcattctgat agagagtgcc
      781 tgtataaagc tgaagtgaca atgaccggga agggaacccg ttttcaggaa tggatgcctg
      841 tctttttcac tgtaaatatc ctggcattac taatgcctgg atatttacag aagagcagca
      901 taaccatgac ggaacggggt taagtcaggt tgtccttaac gctattttca acctcgtatg
      961 ccttcttcag gtttatgttc agatctcata tctctctcag caatcctcaa taatcagata
     1021 caccgctttc accggaccat gaaccccgac gactttgata agctcaatat ccgccgttga
     1081 gctgggacca ctgatgatgt taatacatga cggcattcgt tcaccggcct gcgctttctg
     1141 atgcaatttt tctgcgagtt gcgctacacg cggcaggatt gtgcttttac gcaggataaa
     1201 aagagaagat tccgggagca ggctcaatga acgcccgcgc tcggcggcgg aaaaaagaac
     1261 cacgcctccc gattcggtta aaccatattc agcatacacg acacccactt tagcctgctc
     1321 tgcctgcgag atattctcgg cacctttcgc cggatcccaa acaacggcat tgcattcctg
     1381 ctgcaaacgt tcactaatcc ccaattcctc cagcctcgtg tcaccgctaa tcacgaccga
     1441 ctgatctccc agctctttac acagacgtat tgcagcttct gccgccttcg cctcgctggt
     1501 cagctcacag cgcgtcaaca taacatcgct ggcaaactga ataaacgcgt cacagcgctg
     1561 ctgttggtta agttgggtaa gccgctcgtt agcatagttg ttaagcggcg catcttctgc
     1621 ttgcggttca agtcgcagcg ggcgacccag tgcctgagca acgttattca aaaattcgct
     1681 ccgattatcc attctttttc tcctgcgcct gatgtttctt aaaccaacta cggaaactct
     1741 ctccgtcagc ttcaggaaga tcgcgtgctt ccatccagtc gctaatcgcg ccaaatttga
     1801 gtggtgtttt gccgccattg ataaaccagc ttgccgcatg agcgccggcc atcatcccga
     1861 ctttccacaa tcctggatga ctattggcat aagcgaacat ttttatcgcc cgttgctctg
     1921 cttttgcggt gatccctttt tcagccatca cccgacgatg acgcaaaatc agttttgaca
     1981 gcggaatacg caccggacac acgctgtcac aagcagtgca taaagagcag gcgtagggta
     2041 aatctttaaa atctttatag ccgccaagta gcggagaaat caccgcacca attggccctg
     2101 gataaataga gccatatcca tgaccgccaa tatggcgata tgccggacaa gtattcatac
     2161 aagccccgca gcgaatacag cgcagcacat cccgaaattc agaggccagc acctcagaac
     2221 gcccgttatc gacaataacc agatgaaact cttcaggacc atcaacgtgc cccgcttcgc
     2281 gcggccctgt cagccaggtg ttgtatcccg tcaaacgtgc accaacggca ctgcgcgcca
     2341 gcatggtgat caatacatct acctcggcaa acgtgggggc aatacgctcc attcccatca
     2401 ctgcaatatg cgttttaggc agcgtggtac acattcgcgc attaccttca ttggtcacca
     2461 ggcataccga accggtctct gccaccgcga aattacagcc ggtaatacct atttcagcac
     2521 tgaggaaatc ttcacggatt ttttgccgga tgaataaggt catcgcttca ggcgtttccg
     2581 gcccctcata gcccagacgt tcgtgtagca ctcgacggat ctgatggcga tctttatgaa
     2641 ttgccgggac cacaacatga gatggcggat cttgatccag ctggagaata tattcaccca
     2701 gatcggtttc aatcacctga atgccagcat cctgcaacac atgattgaca ccaatctctt
     2761 cggtcaccat cgatttagat ttcaccacct tccgggcatt tttgcgttgg gcaacctgta
     2821 aaatgtagcg ggtagcgtct tctttggttt ttgcaaaata gacgtgaccg ccgttttgcg
     2881 tcactttttc tgagagctgg tacagataag cgtcgagatt actcagaaca tgatcacgta
     2941 tctgggcggc ccgatcgcgc cactcctccc agtgccccaa ttcatcgacc attttttgcc
     3001 gatttgcccc aatacgctgc tgcgcgcttg ccaccgcttt gcgcatgatc ggatcttcaa
     3061 tttgctgacg gatgcgtgtc ttaaaatctg tattactggt tttgatcgac atctttatat
     3121 cctcagcggc tcatcaacac ttcagcaata tgcatcactt tgactttctg cccttcccgt
     3181 tgtaatcgcc cactgatgtt cagcaggcaa ctcacgtcag caccaattaa atactcaggg
     3241 cggacttcca tcaggtgcac aaccttttct ttcaccatct cgccggatat ttcggccatt
     3301 ttgaccgaga acgtgccgcc aaatccgcag caggtatcct gttcagcaaa ggtcaacagc
     3361 tccagtccac gcacattttt cagcagcgta agtggctcgt ccttcactcc cagcttacgg
     3421 gccaggctac aagatgggtg atacacagct ctcccttgca aactggcacc tacatcgact
     3481 acccctaatt tattaacaat aaaagaggtg agatcctgca tacgcgcggc aaccttttcg
     3541 gcacgtgatg cccattcagg ttcatccgcc agatacgtcg ggtaactttt tatggcatag
     3601 gtgcaagagc cagccggtga aataatggga tcgtcgttat cctccagtgc ggcgatcaga
     3661 tttttcatcc ctggaatcgc ttctttgata taaccgctat tgatcgcagg ctgaccgcag
     3721 catccctgtt tctccgggaa atttacgcga cagccgagtt tttccagtag cagcacggag
     3781 tctcgtgcca ttcttgattt cagggcgtca ccaatacagg tgacaaagaa attgacattc
     3841 acaactaata ctccattact tcatgcccat ttatgcccga tgtttccccc ccaccggggg
     3901 atcaatcatc aataaatatg gcgttctttt ttaattggct agcaacatac cacccctcat
     3961 ttgtatgaca aatgataatc tttcatccaa acttcaaatg caataactaa ttttatttct
     4021 ttcggcatcg ctcacatttt atggggaaat cgacgatcat ttttggcata agaaatccat
     4081 ttaaaaatgg ctacatccct tatacctatt cagagctact tattcagggg ttattgagat
     4141 atacactgat ggtgtacaaa aaaatcaccg acaataatca cgtatcaagt aatagtcgga
//
LOCUS       DAOFMX010000186         4169 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.188, whole genome shotgun sequence.
ACCESSION   DAOFMX010000186 DAOFMX010000000
VERSION     DAOFMX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.188"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..335)
                     /gene="flhe"
                     /locus_tag="QBA20_004219"
     CDS             complement(<1..335)
                     /gene="flhe"
                     /locus_tag="QBA20_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDQ6294516.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGG"
     gene            complement(335..2412)
                     /gene="flhA"
                     /locus_tag="QBA20_004220"
                     /pseudo
     CDS             complement(335..2412)
                     /gene="flhA"
                     /locus_tag="QBA20_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2405..3552)
                     /gene="flhB"
                     /locus_tag="QBA20_004221"
                     /pseudo
     CDS             complement(2405..3552)
                     /gene="flhB"
                     /locus_tag="QBA20_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3788..>4169
                     /locus_tag="QBA20_004222"
     CDS             3788..>4169
                     /locus_tag="QBA20_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDQ6294517.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1022 g          901 t
ORIGIN      
        1 ccaccgcctg gcacttccca gataaatcgc aacggttctg ctgccgctat accggaaaag
       61 gccacggtgg taccgctctg cccctctaat tcgacacaac gagattgcga acacaagcga
      121 acccgcagtc ctgcaggtgt cgggccgata agctgataac gccacgccac cagcgtcatc
      181 aatcctgaag caggttgtcg cgcagaaaga ggtgcagacg acatcgactc accgcgatga
      241 tttagcgtaa ttccgacact acttgcctgc cacatcccct ccccggcggc ttgcaccagc
      301 agcggaaaca ataatatcgc taataaggtt ctcatcattt accgccaatt gtcgccgtca
      361 tgcggatatg tcggttatca gacagttcca gattcgacag gaccactaac tgcggcaagc
      421 tgcggcgcag gaagcgagac aataatggtc gcagcgcgtg gttcaccaac aatactggcg
      481 gcgcacccag catctcctga cgggatagag cttcctgagt ttgcgccagt aaacgatccg
      541 ccagccctgg ctccagtcct cccccgccct gcagcgcctg tagtaacaaa cgttccagcg
      601 gtgtatcgag gccaataaca tggacttcat ctttgccagg aaaccactgc tgggtaatcg
      661 cccgtcccaa cgccacgcgc acgacggcgg ttaattcatg tggatcgctt tggatgggcg
      721 catgttccgc cagcgtttcg agaatggtgc gcatatcgcg aatcggtact ttttcatcga
      781 ggagattttg cagcacttta tgcagtgtgg tgagcgtgac gacgccagga acgagatctt
      841 ccgtcagctt tggcatctcc tgggcgacgc gatccaacag ctgttgcgcc tcctgacgac
      901 caaacagctc tgcggcatgc tggctaatga ggtggttaag atgcgttgct accaccgtgc
      961 tggcctcaac cactgtgtac ccctgaatct gcgcctgttc ttttagcgca ctttcaatcc
     1021 agatagcatt caggccaaat gccggatcga cggtcgcctc accaggtaac gtcccggcag
     1081 cggttccagg gttaatcgcc agccagcgcc ccggataagc atcaccactg ccaatctcca
     1141 cgcctttcat caaaatgcga tagcgggcag gttgcagatc catattgtcg cgaatgtgca
     1201 ccactggcgg cagaaatccc atctcctggg caaatttctt gcggatactg cgtatacggc
     1261 ccaacaactc accatcctgc tggaaatcga ccatcgggat cagtcgataa cccacttcca
     1321 ttcccagaga atcttccagt tgtacatcgt tccacgtcgc ttcgacaacg gtattattct
     1381 ctgccatttt taccggtttg ggttcggcag gcgctttttg ttcgcgtccg cgtatccacc
     1441 aggccagccc cagcaatccg gcagtgaaca gcaaaaatac caggttcggc attccaggca
     1501 ccaggccgag taaaccgagc acggcggcgc ttaacaacat aacgcttggg ttactgaaaa
     1561 gctgattcac catctgctcg ccaacatcct gatcggtgct gacacgcgta acgatgcccc
     1621 ccgcggcggt agaaatcacc agcgccggaa tttgtgccac cagaccgtcg ccaatggtca
     1681 atagcgtata actttccgcc gcgtgtccca tgctcatgcc agttgcagca cgccgaccag
     1741 caacccgccg acaacgttaa tgaccatgat gaggatcccg gcaatggcat cgccgcgaac
     1801 aaacttactt gccccgtcca ttgagccgta aaaatcggct tcctgagtca cttcggagcg
     1861 gcgttttttc gcctcatctt caccaatcaa tccagcgtta aggtcggcgt caatcgccat
     1921 ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc acttctgcta tacgcccggc
     1981 acctttggta atgaccataa agttgatgat cacgagaatg acaaacacca cgataccgat
     2041 agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg accaccttcc cagccgccgc
     2101 cgcgccggta tgcccttcca tcaaaatgat acgggttgaa gccacgttaa gtgccagacg
     2161 caacaacgta gtaaacaaca gaatggtcgg aaacgcagca aactcaagcg tgcgctgggt
     2221 aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa acaacaggtc
     2281 gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga tcaaaatcgg
     2341 tccggcaagg atctgccatt atgtcgattt caggtttgcg ggcaggcgca gcatcgcggc
     2401 cagattactc atgggtcggt ttctcgttaa taaaatccag ggctgccggc accggaagat
     2461 gagtaggtgt acagggcgct gtccaccagc cagacgccag cgtttcagtt gccagaccca
     2521 ggccagcact tccgccaccg cggcgtacag ttgacccggg atttgttgac caatctccgc
     2581 atgtcgatac agcgctcgcg ccagcggcgg cgcttcaagc gtcgggacgt tattttcagc
     2641 gccaatttca cgaatgcgca gcgcgaccag ccctgcacct ttagcgacca ctttcggtgc
     2701 gctcattttg ttttcgtcat actgcaacgc taccgaatag tgggtcgggt tattgacaat
     2761 gacatccgct ttcggcacat cggccatcat ccgacgccgt gcggcagctc gctgcatctg
     2821 acagatccgt cctttaacat gggggtcgcc ttcgctttgt ttgaactcat cacgaatatc
     2881 ctgccgcgac attcgcagct ttttcaggtg gctgaagatt tggaaaaaga cgtcaaatcc
     2941 caccattgga atgacaccaa gcaccaccag cagtgcgcat agccctacca aatccatcgc
     3001 attacccatg gcggtaatcg gtgactcggc cattaagcgc atcatctgcg gccagtgatg
     3061 ccagagaaaa aaccccgtca cgctgccaac caggatggtt ttcaaaattg ccttaagcaa
     3121 ctccgcgcta gtctgtgccg agaatatccg tttaatgccc ggtagcgggt tgagtttgga
     3181 aaacttcggc tgcaaggatt tgccgctaaa taccagcccc cccagcataa ccggagaaat
     3241 gagcgccacc agcaccacgc cgctaatcag cggtagcagc gccagcatgg cttctctgat
     3301 cagcagaata atttgcccga ggatcagatt cgggtctttg ataatactgt gatcaaaatg
     3361 cagcccagcg gagagcatgc ccgacaatcg acgggccagc gacacaccgc caaaccagat
     3421 aacactaacg cccaccagca aaatcagcag tgaggtcagt tcacgggaac gcgggatttg
     3481 cccctcttcc cgcgcttttt ctagtcggtg aggtgtgggg gcttctgttt tgtcgtcgct
     3541 ctcgtcagac acgtcgccaa tcctggaaag agtattaagc gtaaatgatg ccagagcgca
     3601 aagcgttcaa tggtttaagt aaggggcaaa acaggcggga tttagggcgt ttatgcaaaa
     3661 ataggttcag ttgcagaacg ttttgcgaac cattttccag ttctgatatt tagccattta
     3721 gcctatagaa tcgcactccg cctctctgtt agtttgctaa taattaaatt attacaaagg
     3781 tgatcaaatg gaatggcaac aattgcttaa ctgcaaccgt cggaaagata atctaaaaaa
     3841 aaccaacata gcatttttac atccccttgc tagcggaaga caagagatcg agcgggatta
     3901 cgaccgatta ctttttgctg caccaactcg tcggcttgcg gataaaacac aagttttccc
     3961 gttagatcaa aatgacagca tacggacgag gctaactcat tctcacgaag tagcaaactt
     4021 tgctcgcggg ataggaatga ggctcgcttt cgagatgaag gagcacatct ttggcgaaat
     4081 acccacgggg attgtcgttg aacgcgacgt tcctgctctg ttagcagcga tcggactggc
     4141 acacgatctc ggtaatccac catttgggc
//
LOCUS       DAOFMX010000187         4110 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.189, whole genome shotgun sequence.
ACCESSION   DAOFMX010000187 DAOFMX010000000
VERSION     DAOFMX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.189"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(954..1145)
                     /locus_tag="QBA20_004223"
     CDS             complement(954..1145)
                     /locus_tag="QBA20_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HDQ6294518.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1209..1532
                     /locus_tag="QBA20_004224"
     CDS             1209..1532
                     /locus_tag="QBA20_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDQ6294519.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1522..1833
                     /locus_tag="QBA20_004225"
     CDS             1522..1833
                     /locus_tag="QBA20_004225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294520.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1808..2230
                     /locus_tag="QBA20_004226"
                     /pseudo
     CDS             1808..2230
                     /locus_tag="QBA20_004226"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2211..2741
                     /locus_tag="QBA20_004227"
     CDS             2211..2741
                     /locus_tag="QBA20_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HDQ6294521.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2748..2981
                     /locus_tag="QBA20_004228"
     CDS             2748..2981
                     /locus_tag="QBA20_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HDQ6294522.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3527..3994)
                     /locus_tag="QBA20_004229"
     CDS             complement(3527..3994)
                     /locus_tag="QBA20_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294523.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT         1001 a          940 c         1195 g          974 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacaga caagctgtga gcggatgccg ggagcagcat atgtcagagg
      961 ttttcaccgt catccccgaa acgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg
     1021 tgaaacgatt cacagatatc agcctgttca tccgcgtcca gttcgttgag cttctccagc
     1081 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta
     1141 gtcactgatg cctccgtgta agggggattt ctgttcagga ggtaatgata ccgatgaaac
     1201 gcgagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgct
     1261 gtgagggtaa acagctggcg gtatggatgc ggcgggtctg cctgggggag ccggttgccc
     1321 gttccgggaa actgccgaca ctggcaccgc cgttactgtg tcagctggct gccatcggga
     1381 ataacctgaa tcagacagcg aggaaggtga acagcgggca gtggtcttcc ggtaaccggg
     1441 ttcaggtggt ggccgcactg atggccatcg gggacgagct gcgccggctg cgtctggccg
     1501 tcagggaaca ggggacgcgg gatgatagtt aagtttcatg cccggggaaa aggtggtggc
     1561 agtggtccgg ttgattacct gctgggaagg gagcgtaacc gggaaggggc aacggtgctt
     1621 cagggtaatc cggaagaagt ccgggaactc atcgatgcca cgccatttgc gaagaaatac
     1681 acgtccggtg ttctgtcgtt cgcggagaag gagctgccgc cgggaggacg tgaaaaagtg
     1741 atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa aaaatcagta cagcatcctg
     1801 tgggtggatg caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga
     1861 gctacagacc ggaaaacgcc tccagccgta ctacgaccgc gcagacaggc ctagaattga
     1921 tgcctggcag acgctggtta atcaccatta cgggctgcat gacccgaacg ccccggagaa
     1981 ccgcaggacg ctgacactcc ctgataatct gccggaaacg aaacaggcga ttgctgaggg
     2041 cgttacacgc gggatagacg cgctttacca tgccggagag ataaaaggcc gtcaggatgt
     2101 gattcaggct ctcacagagg cagggattga cgtggttagg gtgacgcgaa gcagtatcag
     2161 cattgcagac ccgaacggcg ggaagaatat cagactgaaa ggagcgtttt atgagcaatc
     2221 ttttacagac gggtgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga
     2281 aaatgctgaa cgacgagttc aggaagctcg aagaatctgt aaacagggaa ctgacctcaa
     2341 acgaatcttt aatcaggaac gctatcaacg accatacatc agccctgaaa gaacttctgg
     2401 agaggtatca gaaaacgacc gtcgatacga tggacgcaca ctggaaaacc gtactgaaaa
     2461 tgagcgtgaa acgctggctg tggctgatta tcgtctcagt actgatgttt gccacgaccg
     2521 ggagcctgct gtggtaccag ggcatgaaga taaacgcgaa catgaatatt ctccgcgagc
     2581 agaaagagag tctggagaag ctgaacgcga agacatgggg cgtgcgttat cacgaggaca
     2641 gcaacggacg ttttctggtg ttgcccgagg ggatgaaagc cgaaacgaac tggacgatgg
     2701 aaaacgggaa acagaatgcc gtaaggctgg tacaggagta acagagcatg acggagctgg
     2761 aaagacattt gctgaacgca ttagaacaac tgcaacagga ctatatgcag cggctgaacg
     2821 aatgggagag cgccttcgtg gaattgcaga aaatgttttc gcttacgcaa cgggacaacg
     2881 cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga
     2941 cagaacgctt gagccagtta tacagcgaga acaggagata agggacgaac ggctgataca
     3001 ggaatgcgaa catgcgttat ccctggagcg tgagcaccag ctggaaatac aggaacggac
     3061 actggatggc ccttcgctgg gatggtaatc agagacaggc taaaatgttg tcagcgcgtt
     3121 tccggtgcat tttttgggat aatggttggt tcgggggcac cgggaacgcg cttcagtcag
     3181 accttgctga aaacatagga acgcgttaaa acgcatacgg gcgcgattct ggtgtgtctt
     3241 ttgttgctgt gttttgcctc tattgccaga tcgcgctcat tcccctgaac ttcttttttc
     3301 ccgctcaggt ccccgtcttc ttcgcttccg aaagcatgaa aaaaatccgt ttcagctgtc
     3361 tctggctggc aattcctgcg tgattcacat ggctgcatag ctatgcatgt ggttaaaatt
     3421 taccgggtgc gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg
     3481 ccgtgatcgc gccgaacgcg tttcagcggt gcgtacaatt aagatattac gttaaattgt
     3541 gagggaatgg tccccatata ctgactttat catagggata gggttggtag tgggtcgatt
     3601 gggtttcggc attgtttccg ttaatgccaa ttgcattttt gtatttgatg caacagaagg
     3661 cgtgagattc tcctgggcct ggaaaggata agtgtttaga attgatggca aaatatcttc
     3721 catccgattt ttgaagtagt tgttgtttgg ctgtctccca aagggtgttg ttacaaactt
     3781 cttgaatttg tagtttttgc aggactctta ttactgcgtt atcatgttcc aattctttag
     3841 gggagtgtat tattctctct tttaataagc tgtttaccac agggtttagt tgttgtctaa
     3901 agcacgcaga taatgccatt gctacacaat tttgttcccg aggaagcatg tttggcgggg
     3961 tatgccgtgt ttgatgtgat atgtctattc tcatagaagg tctctggctt atagcatcaa
     4021 caaatacggc tataaaacaa aattttcata gagtgattca agatcttctt gagatccttt
     4081 tttgcggcgc gtaatctgct gccggcaaac
//
LOCUS       DAOFMX010000188         3935 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.190, whole genome shotgun sequence.
ACCESSION   DAOFMX010000188 DAOFMX010000000
VERSION     DAOFMX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.190"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="QBA20_004230"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="QBA20_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HDQ6294524.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="QBA20_004231"
     tRNA            complement(621..695)
                     /locus_tag="QBA20_004231"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="QBA20_004232"
     CDS             complement(769..1701)
                     /locus_tag="QBA20_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDQ6294525.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="QBA20_004233"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="QBA20_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDQ6294526.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3606)
                     /locus_tag="QBA20_004234"
     CDS             complement(2932..3606)
                     /locus_tag="QBA20_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294527.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTKYQNEINQLSALG
                     VNFDDI"
BASE COUNT          985 a          890 c          865 g         1195 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctcaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtccgttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcat
//
LOCUS       DAOFMX010000189         3706 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.191, whole genome shotgun sequence.
ACCESSION   DAOFMX010000189 DAOFMX010000000
VERSION     DAOFMX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.191"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..978
                     /locus_tag="QBA20_004235"
     CDS             <1..978
                     /locus_tag="QBA20_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ6294528.1"
                     /translation="VYPNKYSYVWKPTSRESVTLTAIKDGLAALWKKYEETVNNRDRA
                     MTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVPADSIKPEHIRRYMDKR
                     GEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAKNRERYVTDKEYQAVLS
                     VAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGKTGKKQIKAWSPRLQAA
                     IEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKISGILTDFTFHDLKAKGI
                     SDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPENIPRKYSK"
     gene            complement(1031..1120)
                     /locus_tag="QBA20_004236"
     tRNA            complement(1031..1120)
                     /locus_tag="QBA20_004236"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1084..1086),aa:Ser,seq:cga)
     gene            1214..2011
                     /gene="mtfA"
                     /locus_tag="QBA20_004237"
     CDS             1214..2011
                     /gene="mtfA"
                     /locus_tag="QBA20_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDQ6294529.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2112..2187
                     /locus_tag="QBA20_004238"
     tRNA            2112..2187
                     /locus_tag="QBA20_004238"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2145..2147,aa:Asn,seq:gtt)
     gene            2349..3611
                     /locus_tag="QBA20_004239"
                     /pseudo
     CDS             2349..3611
                     /locus_tag="QBA20_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1029 a          858 c          883 g          936 t
ORIGIN      
        1 gtatacccaa ataaatatag ttatgtatgg aaaccaacat ccagagaatc tgtaacacta
       61 accgccatca aggatggttt agctgcttta tggaaaaagt atgaggaaac tgtaaataat
      121 cgcgatcgtg caatgacatt cggtcgcttg tgggaaaaat tcctcgccag cgcctattac
      181 agtgacctca gtccaagaac acaaaaagat tatctgcaac atcaaaaaaa gttgcttgcc
      241 gtattcggta aggtgccggc agattccata aaaccagaac acatccgtcg atacatggac
      301 aagagagggg aacagagtaa aacgcaagcc aaccatgaaa aaagcagtat gtcccgcgtt
      361 tacagttggg ggtatgagcg agggtacgtg aaggctaacc catgtgcagg tgtaagtaaa
      421 ttcaaggcca aaaaccgcga acgatatgta accgacaaag aataccaggc agtattaagc
      481 gttgcacctc ttcctgtttt tatcgcaatg gaaattgcct atctgtgtgc agcgagggtt
      541 tccgatgtgt tatcgctgaa atgggagcag attggaaacg acgggatctt tatccagcaa
      601 gggaaaacag gaaaaaaaca gataaaagca tggagtccac gattacaggc ggcgatcgaa
      661 aaagcaaaac agttaccaac atccgcctat gtaatcagta atcaatacgg caaccgatat
      721 atgtacaaag gctttaacga aatgtgggta gaagcaagaa atcgcgcagg caaaatttca
      781 ggtattttaa ccgacttcac ctttcatgat ctgaaggcga aaggaatttc agactatgaa
      841 ggaagcagtc gggataagca acttttctct ggtcacaaaa ccgaggggca agtgctaatc
      901 tatgacagga aggttaaagt ttcaccgaca cttgatgtcc cgttacctga aaatattcca
      961 agaaaatatt ccaagtaatt ccaagtgtga tttttgtcac tgacttaatg atgtataagt
     1021 gattgaattt tggcggagag agggggattt gaacccccgg tggagttgcc cccactccgg
     1081 ttttcgagac cggtccgttc agccgctccg gcatctctcc gttcagatgg ttgccatgat
     1141 gccaggaaat ttggcatttt aacagtccct gtccgtgcaa ttttgttcaa gtgacgagtt
     1201 tgcgagcaaa acgatgatta agtggccctg gaaagtacaa gaatcagcac atcaaactgc
     1261 ccttccctgg caggaagcac tatcgatccc ccttttaacg tgtctgacag aacaggaaca
     1321 aagcaaattg gtcgctcttg ccgaacgttt tttacagcaa aaacggcttg ttcctttaca
     1381 gggctttgag ctgaattcat taagaagctg ccggatagca cttctatttt gcctgcccgt
     1441 tctggagtta ggactggaat ggctggatgg ttttcatgaa gtcttaattt atcctgcgcc
     1501 atttgtggtc gatgatgaat gggaagacga tatcggtctg gtgcataacc aacgtattgt
     1561 tcagtcaggt cagagctggc agcaagggcc tatcgttttg aactggttgg atatacaaga
     1621 ttcttttgat gcttctggtt ttaacctgat tattcatgaa gtcgctcata agctggacac
     1681 ccgtaacggc gatcgcgcca gcggagttcc ctttatttcg ttgcgtgagg ttgctggctg
     1741 ggaacacgat cttcatgctg caatgaacaa cattcaggaa gaaatcgaat tagttggtga
     1801 gaatgcggcg agcattgatg cttatgctgc cagtgatcct gctgaatgtt ttgccgtact
     1861 ttctgaatat ttctttagcg ccccagaact ttttgctcct cgtttccctt cattgtggca
     1921 acgtttctgt caattttatc aacaagatcc tttgcagaga ctgcatcacg ctaatgatac
     1981 agactcgttt tcagcgacga atgttcatta attaacaact ttgaagatta attaaccaat
     2041 tgaaatggct tatgaaattt agtgttgaca gacaaggtac cgctaagtaa tatgcgcccc
     2101 gttctcacga ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca
     2161 ctggttcgag tccagtcaga ggagccaatt ttctgttttc atgcatcctt gcgaatcttt
     2221 atctgatgtt gattcaacag gttagtgtga aaactcttct cggtgcattt tgattttacc
     2281 ctacgcatcg ggaaaaattg gtggtcaaat ctgggatcag gttagttcga taatggagtg
     2341 acccccatat atcccttacc gacgcaaaaa tccgccccct caagccttct gataaaccct
     2401 ttaaagtctc cgattctcac ggtctgtatc tgctggtcaa gccgggtggc tcccgccact
     2461 ggtatctcaa ataccgtatt agcggtaaag aatcccgcat tgcgctgggt gcctatccag
     2521 ccatctccct gtctgatgcg cgacagcaac gtgaaggtat ccgtaaaatg ctggcgctga
     2581 atatcaaccc ggtacagcag cgggctgctg aacgtggctc acgaacaccg gagaaagttt
     2641 ttaaaaacat ggcgctggcg tgacataaaa gtaacaggaa atggtcgcag aacaccgccg
     2701 accgtctgct tgccagcctg aacaatcaca tctttccggt catcgggaac ctacctgtat
     2761 cagaacttaa accccgtcat ttcattgacc tgctgaaagg gatcgaggaa aaaggtctgc
     2821 tggaggttgc gtcccgcaca cggcagcacc tgagtaacat aatgcgccat gcggtccatc
     2881 aggagttaat cgatacgaac cctgcagcaa accttggcgg cgtgaccaca cctcctgtca
     2941 gacggcacta tcctgccctg ccgctggagc ggctgcctga actgcttgaa cgtattgggg
     3001 catatcatca gggccgtgaa ctgacccggc atgccgttct gctgatgctg catgtgttca
     3061 ttcgctccag tgaactgcgt ttcgcccgct ggtcagagat tgatttcaca aaccgagtct
     3121 ggacgatacc cgcgacgcga gaacccatta ttggcgtgcg ttattccggc cgcggggcaa
     3181 aaatgcgaat gccgcatatc gtccccctct cagaacagtc catcgccatt ctgaaacaga
     3241 ttaaggatat caccggtaat aatgaactga tcttccccgg cgaccataac ccgtataagc
     3301 caatgtgtga aaacacggtc aataaggcac tgcgggtgat gggttacgac acgaaaaagg
     3361 atatctgcgg tcacggcttc cgggcaatgg catgcagtgc gctgatggaa tcgggtttat
     3421 gggcaaagga cgcagtagaa cgccagatga gtcatcagga gcgcaatacc gtgcgcatgg
     3481 cttatattca taaggcagag cacctggaag cccgcaaagc gatgatgcag tggtggtcgg
     3541 attatctgga agcatgccga gaatcttatg caccgcctta tacaattggt aaaaataagt
     3601 ttatcccata gtatactaac gaatcccaca ctctggcaaa ttaccaccac ctctacagga
     3661 ggtggttcta tatagacttt tatcaacttc atgacgaaaa acacct
//
LOCUS       DAOFMX010000190         3611 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.192, whole genome shotgun sequence.
ACCESSION   DAOFMX010000190 DAOFMX010000000
VERSION     DAOFMX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.192"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            31..510
                     /locus_tag="QBA20_004240"
     CDS             31..510
                     /locus_tag="QBA20_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294530.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
     gene            498..731
                     /locus_tag="QBA20_004241"
     CDS             498..731
                     /locus_tag="QBA20_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDQ6294531.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            731..2197
                     /locus_tag="QBA20_004242"
     CDS             731..2197
                     /locus_tag="QBA20_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HDQ6294532.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            2088..2831
                     /locus_tag="QBA20_004243"
     CDS             2088..2831
                     /locus_tag="QBA20_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDQ6294533.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            2835..>3611
                     /locus_tag="QBA20_004244"
     CDS             2835..>3611
                     /locus_tag="QBA20_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HDQ6294534.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDD"
BASE COUNT          923 a          890 c         1030 g          768 t
ORIGIN      
        1 atcatcaaac tgtcttggat agaagccgca gtggatgcgc acaaaacgtt aaatttcgaa
       61 cccagtggaa gaaagcgtat tggctttgac gtggctgaca gtggtacaga taagtgcgct
      121 aacgtttacc gtcacggatc cgttgttttc tgggccgacg aatggaaggc caaagaagat
      181 gaattactga agagctgcca gcgtacttat caggcggcgc tggagcgtga agcagatatt
      241 gtttacgact ctatcggtgt tggtgcgtct gccggtgcta aattctctga aattaacgct
      301 gaccggaaga gcgagaacgc atacgcgcga cgtgtgaatt accagaggtt taacgccggt
      361 gctggtgtgc atgagccaga tgacgagtac aacggcatcc ccaacaaaga ctttttcgca
      421 aatcttaagg ctcaggcatg gtggctgacc gtttcagaaa tacgtttaac gccattaaca
      481 acggagaaca gtatcctgtg gatgagctga tcagcataga ttctcgttgt ccgttgcttg
      541 aaaagctgaa actggaactg acaacacctc atcgtgattt cgaccgtaac ggacgtgtga
      601 tggtcgaaag taaaaaagac ctcgcaaaac gcgagatacc gtcaccaaac gttgctgatg
      661 cattcattat ggccttcgcg ccaatcgata catcgctgga tatctgggaa cagctgggga
      721 gacaggcctg atggcacgaa acaaacaagc cctgcggcga actgtgcagg ccacagctga
      781 tggttatgag aattttattg cccgagtagg gatgcagaca cctaaccagc actcagcatc
      841 cacctaccgg gctaatttca ccagtcgtaa ccgcatgctg gtggaatggt cctatcgttc
      901 atcctggatc atcggcgaag cagtcgatgc tatcccggat gatatgaccc gcaaaggcat
      961 tcgcatcact tcggaaattg atgcaaaaga tcgtggcatt ctcgaatcac aactggatga
     1021 gttgcaaatc tgggatgcgc tgaatgacgt gctgaaatgg tcgcgcctct acggcggcgc
     1081 ggtgggtttc atcatgattg aggggcaggc accaatgacc ccgctgcgac ccgaaaccat
     1141 cggtaagggc aagtttaagg ggattctccc gctcgaccgc tggatggtcg acccggcact
     1201 gacccgccgc attaaagata tggggccgga cctgggtaaa cctgagtttt acgatgtggt
     1261 gaccacagca acgggaattc ctgcctggcg cattcatcac agtcgcctga ttcgctttga
     1321 tggcgtcacg ttgccatttc agcagaagat gaccgagaac gaatggggaa tgtcggttgt
     1381 agagcgtatc tgggatcgtc ttaccgcgtt cgacagcgct actgtcggcg cggcgcagct
     1441 ggtctacaag gcgcatttgc gtacctacag cgtggagaag ctacgcgagc ttatcgcact
     1501 tggtggtcct gcgtatgaag cgttgctgaa gaatatcgac ctgattcgac agttccagag
     1561 taatgaaggc atgacgctca tggactcgcg ggataagttt gaaacccatc agtacagctt
     1621 cagtggtctg gatgacatcc tttcgcagtt tgcagaacag attagtggcg ctgttggtat
     1681 cccactggtg cggttgttcg gacagtcccc gaaaggattt tctaccggtg atgcagacct
     1741 tgccaactat tacgatcgca tcagttcgtt gcaggagagg cgtttacgtc ttccggtgcg
     1801 gcggatactg gacatcatgc atcgttcgga acttggcaag ccgctgccgg acgatttcac
     1861 gtttgagttt aacccgctct ggcaaatgtc tgatgtcgat cgctcaacgg tggcgttaaa
     1921 caccaccaac gcaatcagta cggcgctggg tgatggtctg atgacactga aagccgctat
     1981 gactgatttg cgagaaaatt ctgacgtaac cggcataggg gcatccatta ccaacgagga
     2041 catcgagaat gccgaagatg aagcgccgcc cggcatcggc gaacctgatg acgaaccgca
     2101 ggagccgtca ggcggaaatc cggtatcgaa ccagcctacg caggatagcg agggcggtcg
     2161 gagacatcgt aaatggtcgc tacgatggtt caaatgacag tatcacggaa attattgcgg
     2221 cgctggagcg ttacagtgaa atcatcaccc cctgggcgac aaaggtcgcg gaaaacttta
     2281 ctgcggacct aacccggcag aacgagaaag tttggcggca acacagcaag aacatcagtc
     2341 gcgagctccg caatcttgtg gaaagcgctc ctgtgggcca ggtgatgcaa tccatcatcg
     2401 ccgaacaggt caagtacatc aaatctctgc ctctcgaggc cgcagacagg gtgtacgaca
     2461 tccagaacaa agcgatagag gctgttgtca ccggtggtcg ggcggagcag tttgctaaag
     2521 agattgcatc taccggcgat gttgctaagt ctagggccga tctgattgcc cgaacggaac
     2581 tgggaagagc aacgggcgcg ctggatatgg cccgagcgat agctattggc tcggatggtt
     2641 atatttggcg tacagccgat gatggcgatg tccgagattc ccacgatcac atgaaaggta
     2701 aattcgtccg ctgggattca cctccaactt tggacggcat gaccggccac gcgggcgagc
     2761 tgccaaactg ccgctgctat aaagagatcg tgtttgttcg cgttccattc gcaatgaaaa
     2821 gggcagcata acccatgaaa tacttttttg agaccaggct cggggaaacc cgataccgcc
     2881 tggctgacgg ctcgttgctg tgcaaagacg tgccgatagg acgaacaggt aagcagctct
     2941 atggtgctga tgacctgcca aaactgaaac ccgataagtt cgttgaaata gtcgtcacgc
     3001 gttctcctga gcaggtattc catccggcca cgcttgcctc attcgaaggg atgagcatca
     3061 cgatcctgca tcctgaagat gaaaacggga atgtgcggct ggtaaatccc gagaactgga
     3121 aagtgcttgc tgtcgggcac ctccagaatg tccggcgcgg gacgggtgag cagtctgatt
     3181 tgatgctggc tgaccttatc gtcaaagacg aaagcgccat tcagcttatc gaagatggcc
     3241 tgcgcgaagt gtcgtgcggc tatgacgcgg agtacgagca gaccgagcca ggtaaagccg
     3301 agcaggtcga tattaccgga aaccatgtgg ctcttgtccc taaaggcaga gccggaaatc
     3361 gttgtgcaat tggagacaga gacacaatgg caaatcaaaa gaaaaactgg tggaaccgca
     3421 tgcgtgcggc catcaagaca ggagatgccg acaccatgaa cgaactggtg gagtcggctc
     3481 ccgcatcggt tacaggagat gagggggatt tgccgcaggg cgttaatctc aacatcaacc
     3541 tgtccccgca gcaaccacta ccggacaaag caccagagat gggcggagat ccaaccggcg
     3601 acagtgatga t
//
LOCUS       DAOFMX010000191         3591 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.193, whole genome shotgun sequence.
ACCESSION   DAOFMX010000191 DAOFMX010000000
VERSION     DAOFMX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.193"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            32..493
                     /locus_tag="QBA20_004245"
     CDS             32..493
                     /locus_tag="QBA20_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDQ6294535.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..1004)
                     /locus_tag="QBA20_004246"
     CDS             complement(534..1004)
                     /locus_tag="QBA20_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDQ6294536.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="QBA20_004247"
     CDS             complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="QBA20_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDQ6294537.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="QBA20_004248"
     CDS             complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="QBA20_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDQ6294538.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          953 a          926 c          818 g          894 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      721 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      781 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      841 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      901 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      961 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta tcatgaggcc ttcaggtgtt
     1021 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
     1081 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
     1141 gttatcttcc agacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
     1201 gcgcagtagt ggtgtacgac tttccaaggt acgtaatgtc aattcggtaa agccctcttt
     1261 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
     1321 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
     1381 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
     1441 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
     1501 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1561 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1621 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1681 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1741 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1801 attcgggtgt aactatcagg ctcacaggcg acgcttattc catagtcatc gccaaaccgt
     1861 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1921 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1981 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     2041 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     2101 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     2161 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     2221 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     2281 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     2341 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     2401 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa ccggtttttg
     2461 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     2521 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2581 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2641 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2701 gtaatcgcga ctgcaccaat atccagctcc tgatacagta cctgagccac tttcatgctg
     2761 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2821 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2881 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2941 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     3001 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     3061 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     3121 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     3181 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     3241 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     3301 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     3361 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     3421 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     3481 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3541 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag a
//
LOCUS       DAOFMX010000192         3548 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.194, whole genome shotgun sequence.
ACCESSION   DAOFMX010000192 DAOFMX010000000
VERSION     DAOFMX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.194"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="QBA20_004249"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="QBA20_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDQ6294539.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="QBA20_004250"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="QBA20_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDQ6294540.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="QBA20_004251"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="QBA20_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDQ6294541.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAOFMX010000193         3431 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.195, whole genome shotgun sequence.
ACCESSION   DAOFMX010000193 DAOFMX010000000
VERSION     DAOFMX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.195"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(123..389)
                     /gene="ospE2"
                     /locus_tag="QBA20_004252"
     CDS             complement(123..389)
                     /gene="ospE2"
                     /locus_tag="QBA20_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HDQ6294542.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(571..972)
                     /locus_tag="QBA20_004253"
                     /pseudo
     CDS             complement(571..972)
                     /locus_tag="QBA20_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1401..2189
                     /gene="virF"
                     /locus_tag="QBA20_004254"
     CDS             1401..2189
                     /gene="virF"
                     /locus_tag="QBA20_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HDQ6294543.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(2182..2553)
                     /locus_tag="QBA20_004255"
                     /pseudo
     CDS             complement(2182..2553)
                     /locus_tag="QBA20_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2552..3306
                     /locus_tag="QBA20_004256"
                     /pseudo
     CDS             2552..3306
                     /locus_tag="QBA20_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          996 a          673 c          703 g         1059 t
ORIGIN      
        1 gatggagtta ctcctctctg aaatagaatt actgctctac tttttctgtt tccagaaatt
       61 attagtaaca aaccttgata tattcttttg aaaatggagc ttaaacgagt ctgaaattaa
      121 cttcagaaat cagaataccg ttggctttgt ttttcatcaa ttccggcttc atttatcttt
      181 gtcaaccatt gcatttctgt agcagtgaat ttcaacgttt tcgtccggtt ggcagcaggt
      241 atttgtaatt cgtatatagc tgctttttta ttgaaaacag tatatccatc tgtagtaaca
      301 gtagaagata ataatacagg atttgatact tcaaggatga tattttcttg tttttgtgga
      361 aggcaaggga atatagtttg tgtaagcata ttgcttcctt atgttatatt ttagtaaaga
      421 atttattttt tacattatag tcggctaaat gaagtttact gtaaaaaaac gatacttttt
      481 tcgtaagcga tgagatgtgg agccagaaaa atggctgcgt tacgtcattg aactgtcctt
      541 gacccacgat attcagacag tcgttgtatc ttaagttgac attacccgct gacgtcgata
      601 ttcccttggt gagagataac ccaacgcact atgcggatgg ttttcattgt aatgctcgat
      661 cgccactgca aggttatgca atgccgttct tggtttcggc atgaacgcga tgcagtcttc
      721 cttcatcgtt ttcatgaacc gttctgctat tccattactc tgagggctac tgactgccgt
      781 cgtgcagggc tccagattca actctctggc gaactgccgt gtttcatgag ccgtataagc
      841 cgaaccgtta tcggccactg gattggccgt tccggtcctt tatcgccata tcgctcctga
      901 catgacatcc tgcaccgttg ctttgtcata atctcccgtg cctgctgccc agtctatggc
      961 ttcacgatcg caatatggaa cgtaacgctg tcatcgggag aatcgtcaat gcggcattcg
     1021 gttaacgctt accaatatca acaccaaccg gatttgtgga gcctccagtc tgaaggggct
     1081 ttatgcgttc cgtataggat attatgatgc tggagttttg cgaagctcca ctcctttccg
     1141 aacatttaca cagatattgc ctaagaaaag tgaaaccata atatggttat agtccctttc
     1201 agtgcaaata cttagcttgt tgcacagaga aatagaagct gcataagctc tttcttcaaa
     1261 aaatgtaaat aaagttaaat ataggaaaaa ttacttaatc tatcttaata acggaaagat
     1321 tttgtataca atcactgtta cagaaatttc ttagttactc tgtaaacact aaatatagtt
     1381 tggtatattc tgttgaattt atgatggata tgggacataa aaacaaaata gatataaagg
     1441 ttcgcttgca taactatatt attttatatg caaaaaggtg ttcaatgacg gttagctcag
     1501 gcaatgaaac tttgactatc gatgaagggc aaattgcttt tatagagcga aatatacaaa
     1561 taaacgtctc cataaaaaaa tctgatagca ttaatccatt tgagattata agccttgaca
     1621 gaaatttatt attaagcatt attagaataa tggaaccaat ttattcattt caacactcct
     1681 attctgagga gaaaaggggg ttaaacaaaa aaatattcct cctctctgag gaggaggttt
     1741 ctatcgattt gttcaaatct ataaaagaga tgcctttcgg caaaagaaag atctatagtt
     1801 tagcttgcct tttatcagct gtttctgatg aggaagcttt atatacttcg atatcgatag
     1861 cttcttctct tagtttttct gatcagataa ggaagattgt tgaaaaaaac atcgagaaga
     1921 gatggcgtct ttctgatatt tcaaataact tgaatttatc agaaatagct gttagaaaac
     1981 gattggagag tgaaaaatta acatttcaac aaatccttct tgatattcgc atgcatcatg
     2041 cagcaaagct tttattgaat agtcaaagct atattaatga tgtatcaaga cttatcggaa
     2101 tatcaagccc atcttatttt ataaggaaat ttaatgaata ttatggtata actccaaaga
     2161 aattttactt atatcataaa aaattttaaa tgcttcatag cccatcgcta ttgccagatg
     2221 ggttttcccc acacctgatg gccccagtaa tacgatattt tcattacgtt ctatgaagct
     2281 gagtgagcgt aacgactgga gttgcttctg cggtgctccg gtggcgaatg tgaagtcata
     2341 ctcttcgaac gttttcaccg ccgggaaggc tgccattcgg gtatacatcg cctgtttacg
     2401 ttgatgacgt gccagttttt cttcatgaag cagatgctcc aggaagtcca tataactcca
     2461 ttcctggtct actgcctgtt gtgacagcgc aggcgctgcg cttatgtact gcacccattt
     2521 tgttggacga tgaaatggaa tagcccctga tatgtcaaag ccaaaatacc cttttgaaaa
     2581 gcgccttgaa gtcgtgaatc actacttcac tactgatgat ggttacagga tcatctcggc
     2641 acgttttggt gtcccccgaa cccaggtcag gacatgggtt gccctctatg aaaaacatgg
     2701 agaaaaaggc ttaattccca aacctaaagg tgttagtgct gatccagagt tgcgtattaa
     2761 ggtcgtgaaa gctgtgattg agcagcacat gtcccttaat caggctgctg ctcactttat
     2821 gcttgctggt agtggttctg tagccaggtg gctgaaggtt tatgaagagc gcggagaagc
     2881 tggtttacgc gcgctcaaga ttggcaccaa aagaaacatt gcaatatcag ttgatccaga
     2941 aaaagcggca tcagcattgg agctgtcaaa agaccgacgc attgaggatc ttgaaaggca
     3001 agttcgattt cttgaaacgc ggcttgtgta tctaaaaaag ctgaaagcct tagctcatcc
     3061 cacgaaaaag tgaaagtact caacgagcta aggcagtttt atcctcttga tgagcttctc
     3121 agggctgcgg agataccgcg cagtacgttt tattaccatc taaaggctct cagcaagcct
     3181 gacaagtatg cggacgttaa aaagcgtatt ggtgagattt atcatgagaa tagaggccga
     3241 tacggatacc gtagggtaac gctgtctctt catcgagaag ggaaacagat taaccataaa
     3301 gctgttcacg tataggaaat tgaaaaacta agcgataccc ctgtccgcgc cttatgccac
     3361 gtgagctttt ctgcttttcc ctccactccg gtgttttctc tctctgcatg gtcgattctt
     3421 ataccgcaac a
//
LOCUS       DAOFMX010000194         3423 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.196, whole genome shotgun sequence.
ACCESSION   DAOFMX010000194 DAOFMX010000000
VERSION     DAOFMX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.196"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(226..429)
                     /locus_tag="QBA20_004257"
     CDS             complement(226..429)
                     /locus_tag="QBA20_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294544.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="QBA20_004258"
     CDS             complement(431..586)
                     /locus_tag="QBA20_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDQ6294545.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="QBA20_004259"
     CDS             complement(789..1196)
                     /locus_tag="QBA20_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6294546.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="QBA20_004260"
     CDS             1273..1500
                     /locus_tag="QBA20_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ6294547.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="QBA20_004261"
     CDS             1484..1906
                     /locus_tag="QBA20_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294548.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2779
                     /locus_tag="QBA20_004262"
                     /pseudo
     CDS             1984..2779
                     /locus_tag="QBA20_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2786..3423
                     /locus_tag="QBA20_004263"
                     /pseudo
     CDS             2786..3423
                     /locus_tag="QBA20_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          887 a          752 c          925 g          859 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccttggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagaggaaga ggaagaggaa gatctaaata ctgatctccc cctaaatccc
     2461 cctcgccaaa aacgagcgtc taaaaaattc gagccggagg ctattgagct gcccgattgg
     2521 ttgccggaaa cactctggca tgagtgggtc cggttcagac aggcattgcg aaaaccgatt
     2581 cgaacggagc agggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
     2641 gcaggtgatt cgacacagca tcgccaatga ataccagggc ctgttcgcgc cgaaaggtgt
     2701 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
     2761 gccaggcttc agggggtaac ggaccatgaa aaatattgcg acaggcggcg ttctggaacg
     2821 catccgccga ctgaccccgc aacatgtaac cgccccattc agaacggtag cggagtggcg
     2881 cgagtggcaa cttgctgaag gccagaaacg tagcgaggag atcaaccgcc tgaatcgcca
     2941 gttgcgggtg gaaaaaattc tgaatcgctc aggcatccag ccgttgcacc gtaaatgctc
     3001 gtttgcgaat taccaggtgc agaacgacgg ccagcgatac gcgttaagcc aggcgaaatc
     3061 catcgccgat gaactgatga ccgggtgtac aaattttgcg ttcagcggaa aacctgatac
     3121 cgggtagaat cacttagcgg cagctatcgg gaatcgcctg ctgaaagacg gtcagacagt
     3181 gattgtggtt accgtggctg atgttatgag cgccctgcac gccagctatg acgacgggca
     3241 gtcaggcgaa aaatttttgc gggagctgtg cgaagtggat ctgctggttc ttgatgaaat
     3301 tggcattcag cgcgagacga aaaacgagca ggtggtgctg caccagattg ttgatcgccg
     3361 gacagcgtcg atgcgcagcg tggggatgct gacaaacctg aactatgagg tgatgaaaac
     3421 att
//
LOCUS       DAOFMX010000195         3326 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.197, whole genome shotgun sequence.
ACCESSION   DAOFMX010000195 DAOFMX010000000
VERSION     DAOFMX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.197"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            454..1281
                     /locus_tag="QBA20_004264"
     CDS             454..1281
                     /locus_tag="QBA20_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDQ6294549.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="QBA20_004265"
     CDS             1325..2074
                     /locus_tag="QBA20_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDQ6294550.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="QBA20_004266"
     CDS             2071..2640
                     /locus_tag="QBA20_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294551.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2764..3006
                     /locus_tag="QBA20_004267"
     CDS             2764..3006
                     /locus_tag="QBA20_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDQ6294552.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLVEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="QBA20_004268"
     CDS             3010..3156
                     /locus_tag="QBA20_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294553.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEDWCHCRG
                     LSVV"
     gene            3165..>3326
                     /gene="xisR"
                     /locus_tag="QBA20_004269"
     CDS             3165..>3326
                     /gene="xisR"
                     /locus_tag="QBA20_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HDQ6294554.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          929 a          797 c          855 g          745 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccgggct gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg gtcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gactggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagcct
//
LOCUS       DAOFMX010000196         3180 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.198, whole genome shotgun sequence.
ACCESSION   DAOFMX010000196 DAOFMX010000000
VERSION     DAOFMX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.198"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="QBA20_004270"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="QBA20_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDQ6294555.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="QBA20_004271"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="QBA20_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          660 c          775 g          944 t
ORIGIN      
        1 aaaaaaaaca tcatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg gggggggacc ctgagcaggt ggtaattgta agacgttgcg
     2821 ggcgacggta ttacaattgc acatcctgtc ctgtttcttt aggacgtatc atggcacagc
     2881 atatcaactg ataaataatc cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg
     2941 cgtcgagtgt ttcttaatca ataattccgc tgatactccg gaaaaacgcg agtctgttct
     3001 catgcctgaa atgcccacac cccacgcaaa aacaagtttt tgctgatttt tctttataaa
     3061 tagagagtta tgacaaatta gttcttcttg ctctctttgt gatatttaaa aaagcggtgt
     3121 cggcgcggtg ttgtagctgc gccaacaccg ctttttaggg gtggtactga ctattttcat
//
LOCUS       DAOFMX010000197         3112 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.199, whole genome shotgun sequence.
ACCESSION   DAOFMX010000197 DAOFMX010000000
VERSION     DAOFMX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.199"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="QBA20_004272"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="QBA20_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HDQ6294556.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="QBA20_004273"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="QBA20_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="QBA20_004274"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="QBA20_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HDQ6294557.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="QBA20_004275"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="QBA20_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HDQ6294558.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="QBA20_004276"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="QBA20_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt gttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatag cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agctaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcaccgctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAOFMX010000198         3068 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.200, whole genome shotgun sequence.
ACCESSION   DAOFMX010000198 DAOFMX010000000
VERSION     DAOFMX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.200"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..497
                     /locus_tag="QBA20_004277"
     CDS             <1..497
                     /locus_tag="QBA20_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6294559.1"
                     /translation="GEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTIP
                     LVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRIDD
                     AESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNVT
                     LDYR"
     gene            577..1272
                     /locus_tag="QBA20_004278"
     CDS             577..1272
                     /locus_tag="QBA20_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDQ6294560.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1301..>3068
                     /locus_tag="QBA20_004279"
     CDS             1301..>3068
                     /locus_tag="QBA20_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDQ6294561.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          887 a          718 c          684 g          779 t
ORIGIN      
        1 atggcgagat tttaaaatcc gcttgtgaaa tcaatgactc tgataagaaa attgaagttg
       61 ctcttggtca ctataatgcc gaacagtttc gttctgttgg cgatcgtagc ccaaaaattc
      121 cattcactat tcctttagtt aactgcccag tgactgggtg ggagcacgat aacggcaacg
      181 ttgaagcgtc tttccgttta tggctggaaa cgcgggataa tggcaccgtc cctaacttcc
      241 ctaatctggc aaaagtgggc tcttttgctg gcacagcgac aacaggtgtg ggtatccgta
      301 ttgacgatgc ggaaagcgga aaccttatgc cactgaatgc tatggggaat gataacacga
      361 tttatcagat cccagcggac tccaatggta ttgtcaatgt tgacctcatc gcttactacg
      421 tatcaaccgt tgaagcatca gaaatcaccc caggggaagc agacgctgtc gttaacgtaa
      481 cgctggatta ccgttaatac gttacggcgt tatctgacct ttcagataac gcccttttcc
      541 ttcctctttc tcgttgtatc aggttgaaaa ataactatgt caaaacgaac attcgcggtg
      601 atattaacct tgttgtgtag cttctgtatc ggccaggcgc ttgcaggggg tatcgtttta
      661 cagcgaacgc gagtgatcta tgatgccagc cgcaaagagg ctgcgttacc tgtcgcaaac
      721 aaaggcgcag aaacgcctta tttactgcaa tcatgggtag ataatataga tggtaaaagc
      781 cgtgccccat ttattataac cccaccgcta tttcgtcttg aggctggcga tgactcatca
      841 ctgcgaatta ttaaaacagc tgataacctg cctgaaaata aagagtcgct gttctacatt
      901 aatgttcgtg ccattccagc aaagaaaaaa tcagatgatg ttaatgctaa cgagttgacg
      961 ctggtattta aaacgcgggt caaaatgttt tatcgccccg cacacctgaa gggacgggta
     1021 aacgatgcgt ggaaatcact ggaatttaaa cgtagtgacc attcactcaa tatatataac
     1081 ccaactgaat attacgtcgt atttgccgga ctggcagtcg ataaaaccga tctcacaagc
     1141 aaaattgaat atatcgcgcc cggagaacat aaacagttac cacttccagc atctggcgga
     1201 aagaacgtga aatgggctgc gatcaacgat tatggcggca gttccgggac agaaactcgt
     1261 ccactgcaat aaaaaatata aaaacacagg ccatcaggga atgccacaac gacaccacca
     1321 gggacataaa cgcacaccga aacagttggc gctcattatc aaacgttgtt tgccgatggt
     1381 gctcactggc agcggcatgc tttgcactac cgctaacgcc gaagagtatt atttcgaccc
     1441 cattatgctg gaaactacaa aaagtggtat gcaaacaacc gatctgtcac gtttttcaaa
     1501 aaaatacgca caactaccag gaacttatca ggttgatatc tggctgaata aaaagaaggt
     1561 ttcacagaaa aaaattacat ttaccgccaa tgcagagcaa cttctgcagc cacagtttac
     1621 ggtagaacaa ctacgtgagc tgggtattaa ggtggatgaa atcccggcgc tggctgaaaa
     1681 agatgacgat agcgtgatca actcgcttga acaaatcatt cccggtacag ctgctgaatt
     1741 tgatttcaat catcagcgac ttaatttgag cattccccaa attgcactgt accgtgatgc
     1801 aagaggttac gtctcccctt ctcgttggga cgatggtata ccaacgctgt ttaccaacta
     1861 ctcgtttaca ggttctgata accgttaccg ccagggcaat cgtagccaac gacagtacct
     1921 gaatatgcaa aatggtgcta attttggccc ctggcgatta cgtaactatt ctacgtggac
     1981 acgcaacgat caggcgtcaa gctggaacac tatcagtagt tatttacaac gtgatatcaa
     2041 ggcgttgaag tctcagttgc ttctgggaga aagcgccacc agcggcagta ttttttccag
     2101 ctacgccttt actggcgtgc aactcgcttc cgacgataat atgttgccaa acagccagcg
     2161 cggatttgcc ccaacggtac gcggtatcgc aaacagtagt gcaatcgtga ctatcaggca
     2221 aaatggttat gtgatctatc aaagcaacgt gccagcgggt gcctttgaaa ttaacgatct
     2281 ctacccctct tccaacagcg gcgatttaga agtcacgatt gaagaaagtg acggtacaca
     2341 acgtcgcttt atccagcctt attcttcatt acccatgatg cagcgacctg ggcatctaaa
     2401 atatagcgcg accgctggac gctatcgcgc tgatgcaaac agtgatagca aggaacccga
     2461 atttgctgaa gccacggcaa tatatggttt gaataatact tttacgctgt atggcggcct
     2521 gctcggttct gaagattatt atgcgctggg gatcggtatc ggcggcacac ttggcgcact
     2581 gggcgcgttg tcgatggata tcaacagagc tgacacccaa ttcgataacc agcactcttt
     2641 tcatggctat caatggcgta ctcagtacat caaagatatc ccggaaacca acaccaatat
     2701 cgctgttagc tactatcgct ataccaacga tggctatttt agttttgatg aagccaatac
     2761 ccgcaattgg ggctataaca gtcgccaaaa aagtgaaatt caattcaaca tcagccagac
     2821 aatatttgat ggggtaagtc tgtatgcctc cggttcacag caagactatt ggggcaataa
     2881 cgagaaaaac agtaatatct ctgttggggt ttccggccag caatggggaa ttggttacag
     2941 cctgaattat caatacagcc gctacactga tgaaaataat gaccgtgcac tctctttgaa
     3001 tctcagtatt ccgttagaac gctggtggat ttgcccctat atttccagac atctgttatc
     3061 acttaacc
//
LOCUS       DAOFMX010000199         3065 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.201, whole genome shotgun sequence.
ACCESSION   DAOFMX010000199 DAOFMX010000000
VERSION     DAOFMX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.201"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..197
                     /locus_tag="QBA20_004280"
     CDS             <1..197
                     /locus_tag="QBA20_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDQ6294562.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="QBA20_004281"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="QBA20_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDQ6294563.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="QBA20_004282"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="QBA20_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDQ6294564.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="QBA20_004283"
     CDS             1821..2576
                     /locus_tag="QBA20_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDQ6294565.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            2977..>3065
                     /locus_tag="QBA20_004284"
     CDS             2977..>3065
                     /locus_tag="QBA20_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDQ6294566.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVF"
BASE COUNT          798 a          683 c          726 g          858 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac caccggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta
     2701 attttagata tacatataac ttgttctatt aataaatcac ctcgtattct gactcggccc
     2761 ctggcgcagc aaagtcaggc atttatactc tttttaacac atagaaaatt catatcaata
     2821 taataaaaac ataaaatatt tgcatataaa tataatctta aagtttagtc tatttaatgt
     2881 tcaatgaaat atttctacct gtataatctt taaagatgtt gaacatatat tcacattaaa
     2941 tatgattatg tacttgttac aaggataagg ttataaatga ataaagttac aaaaacagct
     3001 attgtgggct tacttgcgct tttcgcaggt aatgccgctg caaccgatgg tgaaatagtt
     3061 tttga
//
LOCUS       DAOFMX010000200         2985 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.202, whole genome shotgun sequence.
ACCESSION   DAOFMX010000200 DAOFMX010000000
VERSION     DAOFMX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.202"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..2903
                     /locus_tag="QBA20_004285"
     rRNA            1..2903
                     /locus_tag="QBA20_004285"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          790 a          651 c          933 g          611 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc acatactgtg
      361 agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg accatcctcc
      421 aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag
      481 aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc agtgggagca
      541 cgcttaggcg tgtgactgcg taccttttgt ataatgggtc agcgacttat attctgtagc
      601 aaggttaacc gaatagggga gccgaaggga aaccgagtct taactgggcg ttaagttgca
      661 gggtatagac ccgaaacccg gtgatctagc catgggcagg ttgaaggttg ggtaacacta
      721 actggaggac cgaaccgact aatgttgaaa aattagcgga tgacttgtgg ctgggggtga
      781 aaggccaatc aaaccgggag atagctggtt ctccccgaaa gctatttagg tagcgcctcg
      841 tgaattcatc tccgggggta gagcactgtt tcggcaaggg ggtcatcccg acttaccaac
      901 ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga gacacacggc gggtgctaac
      961 gtccgtcgtg aagagggaaa caacccagac cgccagctaa ggtcccaaag tcatggttaa
     1021 gtgggaaacg atgtgggaag gcccagacag ccaggatgtt ggcttagaag cagccatcat
     1081 ttaaagaaag cgtaatagct cactggtcga gtcggcctgc gcggaagatg taacggggct
     1141 aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt gttgggtagg ggagcgttct
     1201 gtaagcctgt gaaggtgtgc tgtgaggtat gctggaggta tcagaagtgc gaatgctgac
     1261 ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg ttcctgtcca
     1321 acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg tagtcgatgg
     1381 gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga gaaggctatg
     1441 ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag gcaaatccgg
     1501 aaaatcaagg ccgaggcgtg atgacgaggc actacggtgc tgaagcaaca aatgccctgc
     1561 ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac cgacacaggt
     1621 ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact aggcaaaatg
     1681 gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg aagcgacttg ctcgtggagc
     1741 tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca gcactgtgca
     1801 aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt taattgatgg
     1861 ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt aactataacg
     1921 gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat ggcgtaatga
     1981 tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa gatgcagtgt
     2041 acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac tgaacattga
     2101 gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct gcatggagcc
     2161 gaccttgaaa taccaccctt taatgtttga tgttctaacg tggacccgtg atccgggttg
     2221 cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag taacggagga
     2281 gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg cataagccag
     2341 cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg atccggtggt
     2401 tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa caggctgata
     2461 ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct catcacatcc
     2521 tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt acgcgagctg
     2581 ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg agaactgagg
     2641 ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc gggttgtcat
     2701 gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag catctaagca
     2761 cgaaacttgc cccgagatga gttctccctg accctttaag ggtcctgaag gaacgttgaa
     2821 gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct aaccggtact
     2881 aatgaaccgt gaggcttaac cttacaacgc cgaagatgtt ttggcggatt gagagaagat
     2941 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgat
//
LOCUS       DAOFMX010000201         2951 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.203, whole genome shotgun sequence.
ACCESSION   DAOFMX010000201 DAOFMX010000000
VERSION     DAOFMX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.203"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="QBA20_004286"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="QBA20_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDQ6294567.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="QBA20_004287"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="QBA20_004287"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDQ6294568.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="QBA20_004288"
     CDS             2603..>2951
                     /locus_tag="QBA20_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDQ6294569.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          702 c          779 g          812 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAOFMX010000202         2888 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.204, whole genome shotgun sequence.
ACCESSION   DAOFMX010000202 DAOFMX010000000
VERSION     DAOFMX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.204"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="QBA20_004289"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="QBA20_004289"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDQ6294570.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="QBA20_004290"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="QBA20_004290"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDQ6294571.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1132..1890
                     /gene="tam"
                     /locus_tag="QBA20_004291"
     CDS             1132..1890
                     /gene="tam"
                     /locus_tag="QBA20_004291"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDQ6294572.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1894..2808)
                     /locus_tag="QBA20_004292"
                     /pseudo
     CDS             complement(1894..2808)
                     /locus_tag="QBA20_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
BASE COUNT          759 a          667 c          770 g          692 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg gtccgactac cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aatgtctgac
     1141 tggaacccct ctttatatct gcacttttcc gctgaacgat cgcgtccggc ggtggagctg
     1201 cttgccagag tgccgctgga gaatgtcgaa tatgttgccg atcttggctg tggcccaggt
     1261 aacagcaccg ccctgctgca tcaacgttgg cctgcggcca ggataacagg catcgactcg
     1321 tctccggcga tgattgctga agcgcgcagt gctttgccag actgccagtt cgtggaagca
     1381 gacatccgca actggcaacc ggaacaggca ctcgatctga tttttgctaa tgcctcactg
     1441 caatggctgc ccgaccacta cgaattgttt cctcacttgg tttctttact taatccgcag
     1501 ggcgtgctgg cagtacagat gccagataac tggctggagc cgacccatgt gctcatgcgc
     1561 gaagttgctt gggaacaaaa ctacccggat cgcgggcgtg agccgttggc tggcgttcat
     1621 gcttactacg atattttgag cgaagccgga tgtgaggtcg atatctggcg aaccacttac
     1681 tatcaccaga tgccgtcgca ccaggcgatt atcgattggg tgaccgccac tggattacgt
     1741 ccgtggttac aggatctgac cgagagcgaa cagcagcttt ttcttacgcg ctaccatcag
     1801 atgctggaag agcagtatcc actgcaagag aacggacaga tactgctggc atttccgcgt
     1861 ctgtttattg ttgcccgccg tacggagtaa ttatcacgtc agctggtaat gacgatcggg
     1921 aagaattttt gctggaattt ctgcttcatc gttcatctgt aacaggtcaa tttcaatagc
     1981 gttgcagatg gcatccagcg gtaaatcatt gttttcagta ccgaacggat cttccagttc
     2041 ttccgccaga caatccagcg aaataaaagt gtaggaaatc agcacagaga taaaaggcgt
     2101 catgtaatgc aggtccacga ccagcgcgaa cggcagcatg atacaaaaca gataaacggt
     2161 acgatgcaaa atcagggtgt aggcaaaggg aattggcgtg taggcaatgc gctcacagcc
     2221 tgccagaacc gctgaaatat cattaagccg atcgttgagg ctaataaaca ggatatctga
     2281 aagctgtcca ttgcggcgct gaaccgccaa ccattctccc attattaaca agatacggtt
     2341 agctggagag ttcgaagcca gtacacgctg aagatctcca gtcttgagat aatgagccag
     2401 cacttccgcc tgtggctgtt tgcgtaatgt catgcgtaaa cagtgggcga aggcgatttg
     2461 cagcctggca aactccctta cacttgccga atccggcaat gtcgttttta cctctcgcag
     2521 taacgaccgt gaggcaatca tcaactgccc ccaaagtttt cgcgcttcaa cgtaacgggc
     2581 gtacccggca ttattacgaa aaccaagaaa aatggcgatg gcgacaccga gaatgctgaa
     2641 cggtgcgagg gtgaatttga tgcccagatg cgtgtactag ggcagcatga atataacagc
     2701 aatagaaaag agaaaattga gtagtaagcg cgaggatatc ttggataata ctgagccgtg
     2761 ccagacaaaa atacggcgca gccagtgttg ttgtggacga acaatcatgg ttatcttcag
     2821 gcgtggaaaa gtcgccctat taaacgtgat tacgatcaca ttctcaagac gctacttaca
     2881 aattacct
//
LOCUS       DAOFMX010000203         2807 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.205, whole genome shotgun sequence.
ACCESSION   DAOFMX010000203 DAOFMX010000000
VERSION     DAOFMX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.205"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            6..81
                     /locus_tag="QBA20_004293"
     tRNA            6..81
                     /locus_tag="QBA20_004293"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="QBA20_004294"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="QBA20_004294"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="QBA20_004295"
     CDS             complement(299..1057)
                     /locus_tag="QBA20_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDQ6294573.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="QBA20_004296"
     CDS             complement(1065..2168)
                     /locus_tag="QBA20_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDQ6294574.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="QBA20_004297"
     CDS             complement(2178..>2807)
                     /locus_tag="QBA20_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDQ6294575.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMIMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          738 c          675 g          631 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tgatcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg tggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAOFMX010000204         2771 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.206, whole genome shotgun sequence.
ACCESSION   DAOFMX010000204 DAOFMX010000000
VERSION     DAOFMX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.206"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..170)
                     /locus_tag="QBA20_004298"
     CDS             complement(<1..170)
                     /locus_tag="QBA20_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDQ6294576.1"
                     /translation="MSQQPQQPQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRN
                     VLRDIRKMLEEL"
     gene            complement(1054..2658)
                     /locus_tag="QBA20_004299"
     CDS             complement(1054..2658)
                     /locus_tag="QBA20_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6294577.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQSLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          714 a          637 c          631 g          789 t
ORIGIN      
        1 ttaagttctt cgagcatctt acgaatatca cgaaggacat ttcggtgctc cttgcctgtc
       61 agctcagcaa tttcaactga ggtcatggac acaagctggt cgaggttaat gatgctgatt
      121 tcttgttgtg gttgttgtgg ttgttgtggt tgttgtggtt gttgagacat cgcttgattt
      181 cctttttgtt tgcaccctga agtcgcaccc ccactgacct actcaaagtc cccaagcgaa
      241 gggagaccag caggacaaca ggatgcaaac agaatgactt tgagattgaa tactatgatg
      301 atgacgatgt agctgtgcgg agctactggg agggtctctc taggagccac tctagggtct
      361 ctctagggtc tctctaggca tcaggctata cgaaactaca gggcatccta aagaatgacg
      421 tacagggcca tagaggacca tagaggacca tagagggcca tagagttagc ttctgggggt
      481 aatgcctaga ggagacccta gagagaccta tagagagacc atagagaaga cctatagaga
      541 gaccctagag aagacctata ggtttggtga ttgattatta taaatatcat caccccttaa
      601 ggtattccct tagagttaat ctttaattgg tctttaatta aatcttttat ttaatactta
      661 aagttaatct ctaattgtgt ctttaagtta actttaagta atactataag ttaatcttta
      721 attgtgtctt taagttaact ttaagttaag cccctgtagg gactagagac cataattaag
      781 atgattgtgt cttattgcta ttcacttatc gactgaccca gagcacacag gaagcattca
      841 ctttaagtag aacgcttggt gtctgtaatt ggtcatcccg cccctgaaag gatgcgataa
      901 gttacggtta gttgtcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc
      961 gtattaatta tccttgacaa gtagagaaaa ctatgagtca aaaacatgac cacacaggtc
     1021 tgtagtaagt gtcatcctag actatggtct cctctaggtc tccttataaa catcagccat
     1081 cacattgacc agcctatcaa cctcgacctc tttagcataa cggtcgtacg taatagaccc
     1141 tgaactatgc cccatgatcg cctgtgcata cgccaactcg accccatgtt gttttaactg
     1201 ggtcgcaact gtatgtctaa acgagtgaaa cgctaagccg ctacggtcta cgaggacttt
     1261 aggcaacaac gtccgattaa accatttact cacctgctca ccgtaaccgt tcttcatgag
     1321 acgcagacca tcgaacacca tagcgtcatc accgtcagcg cctctacggg tctctacaag
     1381 cgccataaag tcagccaata taaaaccata ggccccatcg accagaggca cacagcggtc
     1441 actatgagcg ttcttgatgc tcttaccggg tagactacta tcgtcttcat tgatatgaat
     1501 ataaacggtt cccgcctcag tggtcctgac gtctttgacc tgtagttggg ctatttcatt
     1561 gagtctcgcc ccagtgatag cagccaaggc ggtaacataa taatgatacg gcttaccaga
     1621 ggtcttctga gagtatgcct tagctgcgac cagtagttgc cccacctgct ccgtagagaa
     1681 ggcgttacgg gcccctgagg ctttacgctg agggacttta agctccagac cctcagtcat
     1741 gttcttttta atcaagtcat tgcgcacagc ccacctaaag acagcagcca tcttaataag
     1801 atacttgttg ttgatggtaa cgacatccag acaatctgtc ttatcttcac ggctcaacag
     1861 gtcagcaagt gggacatcct tgaagcgctg cttacggttc ctaggtaact gctggagcac
     1921 atcacggacc ctaagcatgt cagccctgtt agcgtctgca ttgagaccca gatggtcgaa
     1981 tatttcgatt aacgcagcgt gtgacgcctt gttttccctt aaggttgcag gcttccagtt
     2041 ctgatagttc tccgcttcgt attgctcaaa gaggctcgct atggtaatgc taggcgtaac
     2101 ttctggtttt acttctggag cattaacaga tagggacaca gagtctgctt ggttatcttg
     2161 cctatcaata tcattaaaaa gatcgacgta atctatcaga gactgactgt ctcccgcttc
     2221 gatacgcctc atacaggctt ttagcacctt cagggcctca ttgatgtaac gatgctggtc
     2281 aatgcttagc ggctcactag ccagagcatc ggtaaggctc tcaccaagtt ctccgtattg
     2341 gtcccggtag atatccctca tgtcaggctc aaagagatct gaacggccca tagacagttc
     2401 acactcagcg atgtccttaa ggtgttccct catttcctcg taggtagcgt tgggtctgtc
     2461 tgcgtgtatt gccttgagtg ctgcctttat gtgccttgag taagccatag cggtgcgtct
     2521 atccttggtc cgtaatgata ttgaggtcat tctatcattg tttgattgtc tgaccctaag
     2581 acggaagtag tagacgccgt tacgctggta gagatagtag actgctggaa gtttgtgact
     2641 actggatagc tgaatcatat gtctgtatgt cctgagaggg ttacacagtg ggttacagta
     2701 agtaactctg caaggaggct taaagccgta tcccttagga ttcagtaggt tggagtactt
     2761 taagaaaggt c
//
LOCUS       DAOFMX010000205         2716 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.207, whole genome shotgun sequence.
ACCESSION   DAOFMX010000205 DAOFMX010000000
VERSION     DAOFMX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.207"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            6..224
                     /locus_tag="QBA20_004300"
     CDS             6..224
                     /locus_tag="QBA20_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HDQ6294578.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="QBA20_004301"
     CDS             226..591
                     /locus_tag="QBA20_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDQ6294579.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            588..1253
                     /locus_tag="QBA20_004302"
     CDS             588..1253
                     /locus_tag="QBA20_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294580.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="QBA20_004303"
     CDS             1253..1618
                     /locus_tag="QBA20_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDQ6294581.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2073..2228
                     /gene="hokD"
                     /locus_tag="QBA20_004304"
     CDS             2073..2228
                     /gene="hokD"
                     /locus_tag="QBA20_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDQ6294582.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          773 a          558 c          697 g          688 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaaaatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga cgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     2161 gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg
     2221 aggagtaaga gaccaggcgg gggagaaatc cctcgccacc tctcatgtgt caggcatcct
     2281 caacgcaccc gcactaaacc cgcttcggcg ggttttttgt tgcgtgctga atacgcaggg
     2341 tgaaaaataa ccatatattt gattatatac acaacaaaaa ataaaagtca ttgtacttgc
     2401 acattaaaca atcaaatata cggcgtgaaa taaatattta tcagattaat atttttgtct
     2461 ctatgtggat ataaccgttt gtacttataa acctgggggc atcgtggaaa aaataaagaa
     2521 actatttagt agcaaatacg cagtcatacg tcgtgatgac ctgtcagtta tagtcgaaat
     2581 ggattacttc cctgaaaccc caaaatcaat aatgtatcgt aatggtcgaa aggcaatttt
     2641 tttaccgatg agggtaagtg acattatggg aaatgataaa ctgctggatg aattgcgagt
     2701 cagagcatcc tgttag
//
LOCUS       DAOFMX010000206         2714 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.208, whole genome shotgun sequence.
ACCESSION   DAOFMX010000206 DAOFMX010000000
VERSION     DAOFMX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.208"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            53..2576
                     /locus_tag="QBA20_004305"
                     /pseudo
     CDS             53..2576
                     /locus_tag="QBA20_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            2587..>2714
                     /locus_tag="QBA20_004306"
     CDS             2587..>2714
                     /locus_tag="QBA20_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6294583.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          727 a          586 c          678 g          723 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
      241 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
      301 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
      361 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
      421 tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata tatccattca
      481 ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg
      541 gattaatgcg ctcaccatca actacgattt ttctggttca catacactac gttcagatta
      601 tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga atattggacc
      661 gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc
      721 cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga
      781 tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac
      841 tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc
      901 aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc agtcagccgt
      961 tccacaaggt gcttttgaga tcaccgatct caacaccgca agtacaggtg gcgatttgga
     1021 cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt atgcttcatt
     1081 ggcgattctt aaacgtgaag gtctgacaga tgttgatgtc agcgtgggtg aattgcgcga
     1141 tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt tttcccacgg
     1201 gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt
     1261 cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa
     1321 ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt caaaactatt
     1381 tgacgacaca gacactagct tgcgcctggt tggctatcgt tactccaccg agggctacta
     1441 taccactcaa tgagtgggca tcgcggcgca acagccctga agacttttgg gaaacaggta
     1501 accgacgtag tcgcgtggag ggaacgctaa cgcagtcgtt ggggagagat tatggcaatt
     1561 tatacctgac attaagccgg caacaatact ggcataccga tgatgtcgaa cgattaatgc
     1621 aatttggcta cagcagtagc tggaagcgtc tctcgtggaa cgtctcctgg agttattcca
     1681 atactgccag acaggggacg gggaacaacc atgccagtga taacaccagt gagcagatct
     1741 acatgctctc tttatctgtt cctttatcgg gctggtgggg taatagttac gccacctatt
     1801 ctgtttcgca aaacgataat tccggtagct cacatcaact cggactcagc ggtacggcgc
     1861 tggaaagaaa taacctttca tggaatttaa tgcagtccta taacagtcat gatgatgagg
     1921 ttggcggtaa tatgtccctg acctatgatg gctcttatgg cacggtgaac ggcagctata
     1981 actacagcca aaattcccag aggctgaatt atggtatcag agggggaatt ctggcacaca
     2041 gcgaaggggt aacgttaagt caggagttag gtgaaactat tgctcttgtt aaagcacctg
     2101 gggccgccgg gttagaaata gataatatgc gcggtgctgc gacggactgg cgcggctata
     2161 cggtcaagac acagctaaac ccttatgatg aaaatcgggt agcaatcagc gataactatt
     2221 tctcgaagtc gaatatagaa cttgataata ccgtcgttac gatggttccc acgcgtggtg
     2281 cagtggttaa agcggagttt gtgactcatg tgggttatcg cgttctcttc agagtgttaa
     2341 atgcaaatgg taaaccggta ccttttggag ccattgctgc gatacaagat gcaagtttgg
     2401 cagattcagg aattgtcggt gaccgtggcg aactttatct ttctggtcta ccagaaaaag
     2461 gacaggttac gttatcctgg ggagaaaacg cctcaacaaa atgcatcttc aattattcat
     2521 tttcgacacc agaaagtgag agcggattaa ttgaacaggg tgtgacatgt cattaaaagg
     2581 atttttatga acaagtatat caaacagtgg tgctttgctg tgtttatgct ctcgttaagt
     2641 agtgtagcac ttgcagctcc taaaggtatc tgtacccctg ataatggcgt atttcacagt
     2701 acgcttgatt tctc
//
LOCUS       DAOFMX010000207         2647 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.209, whole genome shotgun sequence.
ACCESSION   DAOFMX010000207 DAOFMX010000000
VERSION     DAOFMX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.209"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..570
                     /locus_tag="QBA20_004307"
     CDS             <1..570
                     /locus_tag="QBA20_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDQ6294584.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMP
                     VYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKD
                     FEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            571..936
                     /locus_tag="QBA20_004308"
     CDS             571..936
                     /locus_tag="QBA20_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDQ6294585.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            933..1622
                     /locus_tag="QBA20_004309"
     CDS             933..1622
                     /locus_tag="QBA20_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HDQ6294586.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKKT
                     AFSKQSVAKVD"
     gene            1816..1891
                     /locus_tag="QBA20_004310"
     tRNA            1816..1891
                     /locus_tag="QBA20_004310"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1849..1851,aa:Ile,seq:cat)
     gene            1893..1969
                     /locus_tag="QBA20_004311"
     tRNA            1893..1969
                     /locus_tag="QBA20_004311"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1927..1929,aa:Lys,seq:ttt)
     gene            1972..2047
                     /locus_tag="QBA20_004312"
     tRNA            1972..2047
                     /locus_tag="QBA20_004312"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2005..2007,aa:Thr,seq:tgt)
     gene            2051..2125
                     /locus_tag="QBA20_004313"
     tRNA            2051..2125
                     /locus_tag="QBA20_004313"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2084..2086,aa:Gly,seq:tcc)
     gene            2418..2633
                     /locus_tag="QBA20_004314"
     CDS             2418..2633
                     /locus_tag="QBA20_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HDQ6294587.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          693 a          583 c          714 g          657 t
ORIGIN      
        1 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
       61 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
      121 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
      181 ccgctgcctc acgcccacca gcaacagaat ccaccacagg aagagacggt ggtcagcatt
      241 gccgttgatc cggaatctcc ggctcagtat ctccagcgcc agaaaccaca acgggaagag
      301 atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca tgacgtgtgg caatcaggca
      361 gatgatccgc accacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgacgat
      421 ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac tacacgccgg ggtaaaagat
      481 tttgaagaaa aacacggtag tcagttgttg ttgctgattc gttttttaat gcacgcgaga
      541 aattcgggtg ttctgaagtg gaaagcataa atgaccgaac gcatagaatt tgttttgcct
      601 tacccgccaa cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      661 gccggtgagc gttatcgccg tgatgtggcg ctaattgttc gccagcagcg actgaaatta
      721 aacctgtccg gaaggctggc gataaagatt attgcagagc caccggataa gcgccgtcgt
      781 gacctggaca atatcctgaa ggcaccgctg gatgcgctga cgcatgctgg actacttata
      841 gacgacgagc agtttgatga aatcaatatt gtgcgcggtc agctcgttcc tggtgggcgg
      901 ctggggataa aaatcacaga actggggtgc gcatgaataa ccagtattta cagtttgtgc
      961 gtgagcagct cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag
     1021 cctggcaaga gaatgccatg ttcgatacag ggcgttacag gcgtaaaaaa atccggtacc
     1081 gcgatgaagt gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat
     1141 cgctggcgaa ggggacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt
     1201 ggcggcgggc tgttctgtct cttgaagagc actataaagc ctggttgttg tggtgttaca
     1261 gcgggagtat ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta
     1321 atgctcaatc cggtaccaga aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa
     1381 tctggctggc ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatacc
     1441 aggagctggc attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     1501 actgggttgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     1561 tgagaacacg ttcaaaaaaa aagacggcat tttcaaagca aagtgttgca aaagtagatt
     1621 aaaaggcata tattcatgca aatctgatac tttgccgatt ttgtacgtga tggccaaagc
     1681 aaacaaaacc cgcccacaag cgggtttttt gtgccactta tctcggatag acatggtgaa
     1741 tgcgctggtg gaggagataa gggtgatttt tgaatgcttg caacattgat ttcgtaacgt
     1801 tattatcctg cgcccggccc tttagctcag tggtgagagc gagcgactca taatcgccag
     1861 gtcgctggtt caaatccagc aagggccacc aaccgccact agctcatcag gaaagagcgt
     1921 caacccttta agttgagtgt gcgaggttcg agtccccggt ggcggtccag tgccgacttg
     1981 gctcagtagg tagagcaact gacttgtaat cagtaggtca ccagttcgat tccggtagtc
     2041 ggcaccatat gcgggcatcg tataatggct attacctcag ccttccaagc tgatgatgcg
     2101 ggttcgattc ccgctgcccg ctccagtcag agtctttcag tctgcgatga tgggaaatcc
     2161 cggagtgact gaaagacgtt taagttatga atgatcgctt ttttttgcaa aattgctgtg
     2221 cagaaatact aaccttcggg caggcgatca ttcataagca ctctgctttt attccgatta
     2281 actgtgagtg gtttgttgga tagagtgctt tccttactgt atatatcgtt tcgcccgctt
     2341 ttgcggtttt ttcttttcaa atccctttca tttctcagtg taaaactacg ccatccgtta
     2401 tttgcggagg tgaggctatg aaatccatgg acaaaatttc aacgggcatt gcctacggca
     2461 cctccgcagg cagtgctggc tactggtttt tacagtggct tgatcaggtc agtccgtcac
     2521 agtgggctgc gattggtgta ctggggagtc tggttctggg cttcctgact tatctgacaa
     2581 atctgtactt caaaatcaga gaagacaagc gtaaggctgc gagaggtgaa taatgtcgcc
     2641 atcatta
//
LOCUS       DAOFMX010000208         2588 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.210, whole genome shotgun sequence.
ACCESSION   DAOFMX010000208 DAOFMX010000000
VERSION     DAOFMX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.210"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(4..183)
                     /locus_tag="QBA20_004315"
                     /pseudo
     CDS             complement(4..183)
                     /locus_tag="QBA20_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            complement(386..853)
                     /locus_tag="QBA20_004316"
     CDS             complement(386..853)
                     /locus_tag="QBA20_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDQ6294588.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRTQCLK"
     gene            complement(850..1383)
                     /locus_tag="QBA20_004317"
     CDS             complement(850..1383)
                     /locus_tag="QBA20_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDQ6294589.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(1449..2078)
                     /locus_tag="QBA20_004318"
     CDS             complement(1449..2078)
                     /locus_tag="QBA20_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294590.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(2082..2297)
                     /locus_tag="QBA20_004319"
     CDS             complement(2082..2297)
                     /locus_tag="QBA20_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HDQ6294591.1"
                     /translation="MKSMDKMSTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          651 a          678 c          579 g          680 t
ORIGIN      
        1 tcagaccctg ggtactttct tcatgaaact gcggtgttcg agtttgcgat atttcttaca
       61 ggggaacgca agcccctccg cctccgcttt ggatttccga tcagcattaa tgtaatccac
      121 catctcaaga ctgctcatgg tcgggtattc gccagaagga agaacggtta atgatttgtt
      181 catgtcggta tttcctttag aaagttgagc ctgttcgcac agaaaagccg tccccgagag
      241 gtcctcacct atacggcagt tctcaggctc agctttctga aagactcggg attgttacgc
      301 gctgcgatgc gcggtttact gcggacatat aaaagccccg caagtgcgag gctcattaaa
      361 tggactttgt gatttgcaaa aaaatttatt tcaggcactg agtcctgatg tactcctgaa
      421 gcattctcaa tgctgtctgg tcgctgatga ttccgtcccg gataccgaga acgtttcgtc
      481 cagcaactgg agagagttcg acggtggcat cattgcccat gccggaggcg ctggaggttt
      541 cggctgagga tggcacaggg cattttcctt tgacgagcac ccgaccacca ttatcaagct
      601 tgcgccgaag agcatcattt tcagctttcg catcagctaa ctccttcgtg tatttagcat
      661 cgagtgcatc agcatcacgc tggcgctgct gcatgtcagt aatggttgcg ttcgccagct
      721 tcagttctcc ggcgtttttg tcgcgctgct ctttgtaggt aatggcgtta tcacggtaat
      781 gattaacagc ccatgacagg cagacgatga tgcagataac cagagcggag ataatcgcgg
      841 tgactctgct cattgatcta tcccccaaca ggctaatgcg ctttcctggt cacgacgaat
      901 aacctgtcca tagcagttat ttgaacgtat gcggcaatcg cgcccaacat cttttatcca
      961 ccagcgaatc gcctcgcatg cgcccttacg atcaccggca ttcagccgct tataaaacgt
     1021 cgacgggaaa cacttaccgg ggccaatgtt ataggggcaa aatgacgcta tacccgcttt
     1081 ctggggttcg gtcagaggta ctttaatatt gcgctccacc catgccagcg ccttatcacg
     1141 ttcaatagcg ttaacctggt cacatttttc cttcgacagc ttcattcccg gtatgacagg
     1201 cttaccatcc accattgtgg caccacgaca gatggtccat atgccagaac catcacggta
     1261 tgccgttgtg tgattaccct ctttttcgtt cagaaactgg tcaagtatct ggggagcaga
     1321 cgcgcctgcg gcaatcagcg ccagaacagc agccgacagg ccgtatttga tttttgcgct
     1381 catggatatt tatcaggatt tatcggcaac aaataacgag ccaacttata tacgcctttt
     1441 aagataagtc agccccggct ggatccagtc atcaggctct ttcttaaagg gggagtattg
     1501 aaatcacgaa gaagagcctc ccgcaccgca gcatccatgt ctgcaccact ggcaagtgct
     1561 tcaatctctg ccgccacctg caaatacccc atgcaacggc caatgcgttt cataactctg
     1621 gtgttttcgt ctttcaggca tctgatagcg agattaataa ttttcaggca gtgatgatgc
     1681 gatgcccgga caggttttcc gttctcataa acgacgaact caccctcttc acaggaaacc
     1741 ctgaaaggcc gatggggttc ctggtcttta tccggtgcag acgcaataaa atcactttta
     1801 tgctcttcct ctccggcact gttaaccgta atgacgtact caggccgcag gtaagccgcc
     1861 tctttttctg acaatgcggc cggtaaaaac ttttcctgca aaaacttcat aaaagtctcg
     1921 tgagcgactt tgtggcagta atcgtaaatc gccgcataat gctcatcgcg gcgtttgttt
     1981 tcatcttcag aactcaccaa cgccgacaat tttttattaa gctcagtgat ttcattttcc
     2041 aggtgattga atcgctgatt catttcttca tgcttcatta tttactctcc ccgtgcagcc
     2101 ttacgcttgt cttctcttat tttgaaatac agattcgtca gataagtcag aagccccaga
     2161 agcagacttc ccagcacacc aatcgcagcc cactgtgatg gcctgacctg atccaaccac
     2221 tgcaaaaacc agtagccggc actgccggcg gaggtgccgt aggcaatgcc cgttgacatt
     2281 ttgtccatgg atttcatagc ctcacctccg caaataacgg atggcgtagt tttacactga
     2341 gaaatgaaag ggatttgaaa agaaaaaaac gcaaaagcgg gcgaaacgat atatacagta
     2401 aggaaagcac tctatccaac aaaccaccca cagttaatcg gaataaaagc agagtgctta
     2461 tgaatgatcg cacgcccgaa ggttagtatt tctgcacagc aattttgcaa aaaaaggcga
     2521 tcattcataa cttaaacgtc tttcagtcac tccgggattt cccatcatcg cagactgaaa
     2581 gactctga
//
LOCUS       DAOFMX010000209         2398 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.211, whole genome shotgun sequence.
ACCESSION   DAOFMX010000209 DAOFMX010000000
VERSION     DAOFMX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.211"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(729..803)
                     /locus_tag="QBA20_004320"
     tRNA            complement(729..803)
                     /locus_tag="QBA20_004320"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(768..770),aa:Gly,seq:tcc)
     gene            complement(807..882)
                     /locus_tag="QBA20_004321"
     tRNA            complement(807..882)
                     /locus_tag="QBA20_004321"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(847..849),aa:Thr,seq:tgt)
     gene            complement(885..961)
                     /locus_tag="QBA20_004322"
     tRNA            complement(885..961)
                     /locus_tag="QBA20_004322"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(925..927),aa:Arg,seq:tct)
     gene            complement(963..1038)
                     /locus_tag="QBA20_004323"
     tRNA            complement(963..1038)
                     /locus_tag="QBA20_004323"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1003..1005),aa:Ile,seq:cat)
     gene            complement(1215..1904)
                     /locus_tag="QBA20_004324"
     CDS             complement(1215..1904)
                     /locus_tag="QBA20_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HDQ6294592.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1901..2260)
                     /locus_tag="QBA20_004325"
     CDS             complement(1901..2260)
                     /locus_tag="QBA20_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDQ6294593.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(2260..>2398)
                     /locus_tag="QBA20_004326"
     CDS             complement(2260..>2398)
                     /locus_tag="QBA20_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294594.1"
                     /translation="MVPALRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVL
                     R"
BASE COUNT          622 a          650 c          507 g          619 t
ORIGIN      
        1 ggacttttta taccccgcag gggtcataac atgcttgaca acttaatagg ggcccccccc
       61 gacagacctg tgctatcgga aagaaaaaag gccatcagca aaactctgat gacctgaatc
      121 acctttacca atattgtatg aaaaaacacg cacgactaat tgacaataat tttcatttcc
      181 attaaaaaag atcgcgtagc attcttaatt catgaggaac tttacccgcc agcaatctga
      241 gtagcgaaag ctgttcatcc ccaatgtttt tgctggcggg tccttttttc tacggtcctc
      301 ctccccggag gggggcattt cattattttt ctaatcgtta ctggtgagaa ggcggaagaa
      361 aaagccagtt cttcgcccct aaataacagc atgattccag tccatgcaaa atctgatttc
      421 cagctcacac ataccgagcg ctgtacaaaa aaccgccagc caaggcagtt aagagtgtgt
      481 tcccggggtt tgcttagaat attttgtaag ttgtccgaag agatatttac aacaccagaa
      541 tgatgaatca tcagtcccct gccagaggtt cactacgtaa cctgaccgac aatgcaccat
      601 tctggtgctg aaaaaagagc actggaactg caacaaaaca acatcacaga acaggagatg
      661 gtaaggagtc agacattccc acacaatatt gtgtcagtgc aaataacaac cttcgtctca
      721 aatctcgctg gagcgggcag cgggaatcga acccgcatca tcagcttgga aggctgaggt
      781 aatagccatt atacgatgcc cgcatatggt gccgactacc ggaatcgaac tggtgaccta
      841 ctgattacaa gtcagttgct ctgcctgctg agctaagtcg gcgctggccc accaccgagg
      901 actcgaacct cggaccgtca acttagaagg ttgatgctct atccggatga gctagtggtg
      961 gttggtggcc cttgctggac ttgaaccagc gacctggcga ttatgagtcg ctcgctctca
     1021 ccactgagct aaagggccgg aggcagaata ataaccatat gtcatcacat ctgcaaactc
     1081 atctgaccac cagcacgttt aacgtcctgt gccatttttc aggcataaaa aaaccgctcg
     1141 gcggcgggtt ttgcttactt taccatcgcg tacaaaatcg gcaaagtatc agatttgcat
     1201 gagatatatg ccttttaatc tacttttgca atactttgct ttgaaaatgc cgctttttgt
     1261 tctgaacgtg ttctcatcac aaacaataaa gcctcactat ccagtcggtg aaaaatgtgt
     1321 ttcattacaa cccagtgacc agtaaatgtt ttggaccagt ttttggttgt tactcccgcc
     1381 agtaatgcca gctcctgata ttcataacct tccccaccaa aaagttctgc ttttaccgcc
     1441 tgcgccgcca gccagattaa ttttttcagg cgttcctgtg ttttctctgc aattttccta
     1501 gtgccagaat gagccttaaa ctcttcccac gcccagtggg ttatggtgat ctgatactcc
     1561 cagcaaatac ttccgctgta acaccacaac aaccaggctt tatagtgctc ttcaagagac
     1621 agaacagccc gccgccacga tgatgtcgaa aacgcaacct gactgaccag aggaattgac
     1681 gcccccttcg ccagtgattg ttttcccggg attggtggat tatcccgcgt tatcattttt
     1741 ccggtcacct catcgcggta ccggattttt ttacgcctgt aacgccctgt atcgaacatg
     1801 gcattctcct gccaggcttc aagctgacct tttgtcgccc cactcaaatc agcggtggcg
     1861 ataatgagct gctcacgaac aaactgtaaa tactggttat tcatgcacac tccagctctg
     1921 tgatttttat ccccaaccga ccaccaggaa caggcagtcc gcgcacaata ttggtttcat
     1981 caaactgctc gtcgtcgata agcaaccccg catgtgtcag tgcatccagt ggtgctttca
     2041 ggatattgtc caggtcacga cgccgcttat ccggtggctc tgcaataatc tttatcgcca
     2101 gtcttccgga caggcttaat ttcagtcgct gctgacggac aataagcgcc acatcccggc
     2161 gataacgctc accggctttt gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat
     2221 tcaccgtcgg cggataaggt aaaataaact catggcgcat cagcgcagca cctcctgcac
     2281 cagcttttca aactttccga ctctggtttc cagctctgcc acacagtcca ccagctcatc
     2341 tactgctttc tgtgcgcgat gcttcgcctg catcagttcc cgaagcgcgg gtaccata
//
LOCUS       DAOFMX010000210         2389 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.212, whole genome shotgun sequence.
ACCESSION   DAOFMX010000210 DAOFMX010000000
VERSION     DAOFMX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.212"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..107
                     /locus_tag="QBA20_004327"
     CDS             <1..107
                     /locus_tag="QBA20_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDQ6294595.1"
                     /translation="QTHLDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            100..1059
                     /gene="ssuA"
                     /locus_tag="QBA20_004328"
     CDS             100..1059
                     /gene="ssuA"
                     /locus_tag="QBA20_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDQ6294596.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKIAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1056..2201
                     /gene="ssuD"
                     /locus_tag="QBA20_004329"
     CDS             1056..2201
                     /gene="ssuD"
                     /locus_tag="QBA20_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDQ6294597.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2210..>2389
                     /locus_tag="QBA20_004330"
     CDS             2210..>2389
                     /locus_tag="QBA20_004330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HDQ6294598.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          528 a          652 c          672 g          537 t
ORIGIN      
        1 tgcaaaccca tcttgatacc gcgctagaaa ctttctggca ggcattgcac cgccgcgatg
       61 ttcaggttcc tgaccttctg tctctgcgag gtaatgccca tgcgtaacat cattaaactg
      121 gcgctggcgg gattgcttag cgtctctact tttgcggttg ctgcagaatc ctcgcctgaa
      181 gcgttacgta taggctatca gaaaggcagt attggtatgg tgctggcaaa aagccaccag
      241 ttactggaaa aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg
      301 caaatgttgg aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg
      361 ccaatctttg cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag
      421 cccaaagccg aagtgattct ggtggcagaa aacagcccga tcaaaaccgt agccgatctt
      481 aaaggtcaca aaattgcctt tcagaaaggt tccagttcac acaacctttt actgcgtgca
      541 ctgcgtcagg ccggacttaa attcaccgat atccagccca cttacctgac gcctgccgat
      601 gcccgcgccg cgttccagca aggtaacgtt gacgcctggg ctatctggga tccctactac
      661 tccgctgcgt tattacaggg cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa
      721 actggatcgt tttatctggc agctcgtccc tatgcagaaa aaaacggcgc ttttattcag
      781 ggcgtactgg caacctttag tgaggccgat gcgttaaccc gcagccagcg cgaacaaagc
      841 atcgctttac tggcaaaaac gatgggctta ccggcaccgg tgattgcctc ttacttagat
      901 catcgccctc ctaccaccat caaaccggtt aacgccgagg ttgccgcctt acagcagcaa
      961 acggcagatc tgttttatga aaaccgtctg gtgccgaaaa aagtcgatat tcgccagcgc
     1021 atctggcagc ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt
     1081 taccgaccca cggtgacggg cactatctgg gaacggaaga aggttcacgc ccggttgatc
     1141 acggttatct gcaacaaatt gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga
     1201 tcccaacggg gcgatcgtgt gaagatgcgt ggctggttgc agcgtcgatg atcccggtga
     1261 cgcagcggct gaagtttctt gtcgccctgc gtcccagcgt aacctcacct actgttgccg
     1321 cccgccaggc cgccacgctt gaccgtctct caaatggtcg tgcgttgttt aatctggtca
     1381 caggcagcga tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct
     1441 acgaagcctc ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca
     1501 atttcaacgg taaacatatt catgtgcgcg gagcaaaact gctcttcccg ccgattcaac
     1561 agccttatcc tccgctttac tttggcggat cgtcagatgt cgcccaggag ctggcggcag
     1621 aacaagttga tctctacctc acctggggcg aaccgccgga acttgttaaa gagaaaatcg
     1681 aacaagtgcg ggcgaaagct gccgcgtatg ggcgcaaaat tcgtttcggt attcgtctgc
     1741 atgtgattgt tcgtgaaact aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc
     1801 atcttgatga tgagactatc gccaaagcac aagccgcatt cgcccggacg gattccgtag
     1861 gacaacagcg aatggcggcg ctacataacg gcaagcgcga caatctggag atcagcccca
     1921 atttatgggc gggcgttggc ttagtgcgcg gcggtgccgg aacggcgctg gtgggcgatg
     1981 gtcctacggt cgccgcgcga atcaacgaat acgccgcgct tggcatcgac agctttgtgc
     2041 tttcgggcta cccgcatctg gaagaagcgt atcgggttgg cgagttgctg ttcccacatc
     2101 tggatgtcgc cattccggaa attccccagc cgcagccgct gaatccgcaa ggcgaagcgg
     2161 tggcaaatga ttttatcccc cgtaaagtcg cgcaaagcta aggaacacga tgatggcaac
     2221 gccagtgaag aagtggttat tgcgcgttgc cccctggttt ttaccggtgg gcatcgtggc
     2281 ggtgtggcaa ctggcctcct cggttggctg gctgtcgacg cgtattttgc cttcaccgga
     2341 aggagtggtg acggcgttct ggacgctctc cgccagcggc gaactgtgg
//
LOCUS       DAOFMX010000211         2387 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.213, whole genome shotgun sequence.
ACCESSION   DAOFMX010000211 DAOFMX010000000
VERSION     DAOFMX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.213"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="QBA20_004331"
     CDS             complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="QBA20_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDQ6294599.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="QBA20_004332"
     CDS             complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="QBA20_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDQ6294600.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1929..>2387)
                     /locus_tag="QBA20_004333"
     CDS             complement(1929..>2387)
                     /locus_tag="QBA20_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDQ6294601.1"
                     /translation="GGNIDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLF
                     DYLADLFKIDNSHGGRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPA
                     VLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          678 a          635 c          613 g          461 t
ORIGIN      
        1 caggtgtcgc tctgttccca gcaaatgctg atatgcggat aacgtaaaaa ggtgtccaga
       61 ttccacgttt catgcagtgc cggatgatct ttgcgtaacc agacgcaggg tacatcacta
      121 aacagcactt cataatcaat ggctaacggt agcgagctta acagctcccg cgagcgagga
      181 tggctttcgc gaccggaaaa accaatatcc acttcaccac gagtaatggc atctaaggaa
      241 tcgtaatccc agttacgtag tttgatggtc gcctgcgggt aacgttggta gatccgtttc
      301 gacagcgcat taagcatgat catcatcagc ggcgattccg ccgccaactc aaacttcagg
      361 ccgcgcggtg tctggtggtg cggtttatcc agcagcagat tgctcatttg catccattcc
      421 gccagatttt gctccatgct gaccattagc tgtgtgggcg acagccccag cggtgagttc
      481 acaaagagcg ggtcgtcaaa ccacgctctt aactttgcca gcgacttact caccgctgaa
      541 ggggtcacgt tcatccgctt cgccgctttg gtcacgctgc gctcctgcat cagcagttgc
      601 agacagagca gcagattgag atcaagcgtg gtgatggatt tcttcatgag cggcaacggg
      661 gcgtccaggc gcgacgaaca taatcagcaa caggctcact atgctacagg caatcagaat
      721 cccgatcagc atattccatg cgccgatacc aaccaccgcg gccagccaaa tccacagtga
      781 cgaaccgcaa acctgcgcaa tacctaaggt cgagctggct acgcccgcgc gtaatgaaaa
      841 tggtcctaac gcctgactca tcgctacgcc aaaacctacc gagaaaccgg cgcaaatcag
      901 cgtgatacca aacagagaaa ccgcatggga aggtgaaacg gcaagggtga tccccgccgc
      961 aaggaataac acctgcgagg tgatcatcaa cgtacgtggc ttaaaaattc ccagcgcaaa
     1021 tggcgtcgag aatgaaaagg tcatgctgac gccagcggtc agcgccataa tggtggcgta
     1081 ttctccgcgc tcaaacccca tgatttccat cagcaatacc ggcgatgtat tgacgaaagt
     1141 gaggatcacc gaaacgctga gggtggtgat aacaacacgg ctgaggaaaa aacgattaag
     1201 cagcgactcg ctattttctc gtggtttgtc cgaagcagcg ggggacgctg ggcgcgtttc
     1261 ttttaaaata aacaaagaca acatcagtac cgcgatgccc atcattgcca tcgcccagaa
     1321 cagactctgc cacgggaatt taagcataat cagatgtccg agcactggcg ctaacaccgg
     1381 aatgatgcag gtaataccgt tgagtaatga cagcacttta gcccgacgtc gatcatccag
     1441 cgtgtcgcgc aaaatggcga acgccaccac gtaacaacag cctgcgccca acccctgtag
     1501 aaatcggcct gcaagaaata acgtgctggt ttcagccagt gaacagaaca ccgaggcaat
     1561 aataaatagc gccgcgccgg gtatggcgac cggctttctc cctgaacgat cggccacttt
     1621 accggcaaat agcatcgcag ctgccatccc cgccagatat acggagaacg caatatgcaa
     1681 ctgcgcttcg ctggcattga gatcggcggc gatgcgcggt aaaccaacga ggtacatatc
     1741 aatcccggcg ggataaagta aaaccagggc aaaactacaa atcaaaaagc gggacatggg
     1801 ctaaagcgtc ctgatagtaa ggtgacgcaa agcatacgtg gtgaaggagg ataaggcgag
     1861 ttgccatttg gacataagtg atttcctgag aggcaagaag ccagcgaatg gctggcttct
     1921 tgaaggattt agccaaattt aggtaacacg ttaaagacgt tgccgaacca gcacaaaatc
     1981 acggttatac caaagagaat gacaatcgcc ggaataagat tgccgcccca gaccgtgaac
     2041 atctgattgg gaaacttctt gcgagctttg attgcaagca ctgcgggaat aatgaccgcc
     2101 cagatggtgg cgcacagccc ggcaccgccg atcccgtaaa taaagccgtt cgggaagatc
     2161 agatacaaca acgcaggtgg caggaaggtt aacagcacgg ttttgaaacg cccgccgtgg
     2221 gagttatcaa tcttaaacag gtccgccaga taatcgaaca accccagcgt gacaccaaag
     2281 aacgaactgg caacagctaa gttagagaac accagcaggc aaaactcgat aatgccgtgc
     2341 tgtttggtgc cgaggaacga tttcaccagc gaatcaatgt tgccgcc
//
LOCUS       DAOFMX010000212         2370 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.214, whole genome shotgun sequence.
ACCESSION   DAOFMX010000212 DAOFMX010000000
VERSION     DAOFMX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.214"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..823)
                     /locus_tag="QBA20_004334"
     CDS             complement(<1..823)
                     /locus_tag="QBA20_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HDQ6294602.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1421..1669)
                     /locus_tag="QBA20_004335"
     CDS             complement(1421..1669)
                     /locus_tag="QBA20_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HDQ6294603.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2024..>2370)
                     /locus_tag="QBA20_004336"
     CDS             complement(2024..>2370)
                     /locus_tag="QBA20_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDQ6294604.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          613 a          438 c          571 g          748 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg agttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtat atattgaaga acttctgctt tacactatcc taaactggat cggtttaatg
     1021 taaaaaaacg atactttttt ggggggggca atttttcctt aaggtatgaa aaaaggagct
     1081 gaagctatat aatagctatt aatgccactg gtatcagtga atcatgtata taaaaaactg
     1141 tgttaaggag gctgtccatt agcggttttt tattcttctg gaaccgcggc atctttgcgt
     1201 atctttttgt cttctcaccg tctggtcggt gttttgctga gactgctaat ttcctgtctt
     1261 tgctagtgct gtactggtac tgcccaatca tgattggcgg gggcgggcgg agttgagacc
     1321 gcagacacgt cgtatgcaag aacgtgctgc ggctggctgg tgaacttccg atagtgcgag
     1381 tattgaatga tttccagccg ttactgattt tacgtgctaa ttagtgaaca aaccactcgt
     1441 cagcagactc ccaggtatct ttcagagttt cctgaacaaa agttttagct gaatctttat
     1501 cggcggtgcg cgtaacagaa aggccatcgt tgctggtggc ttttacgatc acctctacat
     1561 cgtcataacg tttactgatg cgtcgggtta attcttcctt taacgcatcc acagcaccgg
     1621 ttggcatttt agtcattttc tctttggcta tgcagatttc aaaacgcata aaaatccctc
     1681 tacactgtgt ttttatacag tattattttt aactgtatgg ataaacagtg tcaagagtga
     1741 gggaaagtga ttttttgggg catgcatggg gcaaaaatat ttgttgtggg gcattttggg
     1801 gcatgattgg gacactttgt tacatatgaa ctttgccgat tttcatatga catggcaatc
     1861 caacgctttg attattctaa agaaaacaca tgctcttggg cgttctttag tgatttttaa
     1921 aaatcccgta tcacgcagtt aaagtggcgg gcatactctt caaggctggt gatccccaac
     1981 cgcacccatt taggatgtga ccattgcggt agaccgatgt aaatcataac gcgtgaagga
     2041 tctcttccac gctacgcaca cgggcgatgc gcgggtagat atgattaatg ctgttattgt
     2101 gttgctcggc gctggcggca ctacaggcgt cttcggcaat caccagatta aagccaagtt
     2161 cccaggcatt gcgggcggtg gattcaacac cgatattggt cgagatccca cataacacta
     2221 ttgtatcgat acctcggcgg cgcagttgca actccagatc cgtaccgtaa aacgcacccc
     2281 actgacgttt gatgatttcg atatcactgt cagttgcacc taatgcagct ggatgttgcc
     2341 accagttttc gggcaacact ttagcggggg
//
LOCUS       DAOFMX010000213         2329 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.215, whole genome shotgun sequence.
ACCESSION   DAOFMX010000213 DAOFMX010000000
VERSION     DAOFMX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.215"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(1..213)
                     /locus_tag="QBA20_004337"
                     /pseudo
     CDS             complement(1..213)
                     /locus_tag="QBA20_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            488..792
                     /locus_tag="QBA20_004338"
                     /pseudo
     CDS             488..792
                     /locus_tag="QBA20_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            961..2325
                     /locus_tag="QBA20_004339"
     CDS             961..2325
                     /locus_tag="QBA20_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6294605.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
BASE COUNT          595 a          479 c          540 g          715 t
ORIGIN      
        1 tgaacctcga tcggtgtggt ggattagccc ggcaggtggg cgctggctcc tgagcgccat
       61 aaacagggct ttacccgtca actctttcgt catgcgctct cccatggcgt aaccgacaat
      121 ttcgcacgta taaacatctt tgacgccagc gagatacagc catccttccc gggtggcaac
      181 atacgttata tctgcgaccc agacctgatt aggggggttg gggagcaatg gaacagtaaa
      241 cgccgttaag aagtcaattt aaatagccaa aaattaacta gccaatttac attgcaggtt
      301 gtaattttta accacctttt ttacgatagc caaattacct atacccatcc cggccctgcc
      361 aagccatacc cgctatcggc gatgaaaatg cttttgtgcg cggtcggagt ggtcccgacg
      421 agggttttac ccgaagtcgg ggcgtatctc cgcgttagcg ggccgtgagg gccgcttacg
      481 agcgtgtatg agagacgtac agtggttttg tccgctcaca ccagtacata caggtacgga
      541 aataaaacca gtcgaaatgc cggtaacgac gattactcct gtctctgatg ccggtggact
      601 acaagatttt atttactggc gtcctgatgc ggacaggact ggtatggaac ctgtttatgt
      661 gatacttaat gatcctctgg attccgggcg atttacgcgt aaacaactaa ataaaaatat
      721 cttaaacatg caagtaattt tggtattctc gatacaagaa taaatagtag taactactat
      781 agttgcaaat aatgttaaaa acatttctat gatcatcttt acatttttca tgattgacaa
      841 ttgtttttgg ttaaaaaaag acaactaatg agtgtgataa tgcaatactt tgagtgatta
      901 tataatgata agtaaacagg tttgcgacaa ttggttatca gtattacaga aggattaaat
      961 atgtcattat tcacagaacc taaggaaatt gaaagattac cctctgaaga gattgaacga
     1021 ttatatcccg tcctgcgata cagggtcttt atcagcattt tcctcggata tatggggtat
     1081 tattttgtcc gaaacaccac cagtgtgtta tccggtgtgc tgcacatgtc tgcaaccgag
     1141 atcggcatta tctcctgtgc cggtttttta tcctatggta tatcaaagtt tgtttccgga
     1201 cttatttctg acagaagtaa ttcaaaagta tttttgtctc ttggtttatt cctgtccgga
     1261 ctggtaaatt ttctgatagg atatattccc gggatcatca catccgttac gttattttca
     1321 acaatgtatt tacttaacgg ctggatccaa ggtatgggat atcctcctgg tgccaaaaca
     1381 ctggtgttct ggtatgaaca tcgggaaagg atcacctggg ccacactgtg gaatttatcg
     1441 cacaatgtcg ggggagctct ggcccccgtt ctgattggtt tcagttttgg ttttttcggc
     1501 gacagtgctc tggatcatgc ccgggccgca tttatcttcc caggagtact gtgtatggca
     1561 atgagtgtgc tgatttactt tattcaggtg gacagacctg tatcagtggg gcttcctccc
     1621 attgaagaat ggaaaggtaa cgtcgtttca cacccggcta aaggccgtga acaaggtccg
     1681 agactctcca ttcctgatat catcagaaaa catatcatcc gaaataataa actcatttac
     1741 tgctgtatct atggttcgtt tgtttatatt ctgagatacg gtattgtttc ctgggctcct
     1801 aagtttttaa gtgacagtct ggatgtcggt ggtaaggata tgggaaaact ggcatcaatg
     1861 ggggggggtt ctgtttttga aatcggtggt gtggctggta tgcttcttgc cggatacctg
     1921 tctgtcagac tgttcagaaa ctcaaaacca ttaacaaata ctttatttct ggcacttacc
     1981 attattttat taatcgcgta ctggtacgtg ccatcaggca atgaatatct ctggctaaac
     2041 tatacaatcc ttattctgtt ggggctggca gtgtatggtc cggtgatgtt tattggtctt
     2101 tattcaatgg agcttgttcc taaagaagct gccggagccg catcagggct gtcaggcaca
     2161 ttttcctata tcttcggctc aattgttgcg acactgggaa tgggacttgt tgtcgattat
     2221 ctgggatggg gggccacatt tatcgtactg atactgtctg ctgtttttgc catcattttc
     2281 acactgatga gtcgtgaacg ctcactggag ttcgaaaaag agtaatttt
//
LOCUS       DAOFMX010000214         2325 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.216, whole genome shotgun sequence.
ACCESSION   DAOFMX010000214 DAOFMX010000000
VERSION     DAOFMX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.216"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            61..282
                     /gene="kil"
                     /locus_tag="QBA20_004340"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="QBA20_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDQ6294606.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKADSW"
     gene            276..452
                     /locus_tag="QBA20_004341"
     CDS             276..452
                     /locus_tag="QBA20_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294607.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="QBA20_004342"
     CDS             527..802
                     /locus_tag="QBA20_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294608.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="QBA20_004343"
     CDS             902..>2325
                     /locus_tag="QBA20_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HDQ6294609.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          719 a          531 c          528 g          547 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatgttcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gattcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAOFMX010000215         2228 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.217, whole genome shotgun sequence.
ACCESSION   DAOFMX010000215 DAOFMX010000000
VERSION     DAOFMX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.217"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(142..654)
                     /locus_tag="QBA20_004344"
     CDS             complement(142..654)
                     /locus_tag="QBA20_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HDQ6294610.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            849..1613
                     /locus_tag="QBA20_004345"
     CDS             849..1613
                     /locus_tag="QBA20_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294611.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1564..>2228
                     /locus_tag="QBA20_004346"
     CDS             1564..>2228
                     /locus_tag="QBA20_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDQ6294612.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          637 a          485 c          562 g          544 t
ORIGIN      
        1 cagcattgag aatgcttcag gagtacatca ggatccaatg ccttgggggg tagcggtaat
       61 tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggaa gctggattcg
      121 caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa aactaccaac
      181 ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt tcactggatg
      241 aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga taaagcgcga
      301 agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt ctattgtatt
      361 ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta taccaagatg
      421 gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag cttcccatgg
      481 tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg ctggtggcaa
      541 tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa gcccccatag
      601 gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca tcaatgtctt
      661 tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta ggcttaaaca
      721 ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc aggagtgagt
      781 gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa atcacaggaa
      841 gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg gccggagtcc
      901 ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc aggaagaggg
      961 ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat ggtacgcaaa
     1021 aaaggaaagt gcgtaccagc agaaagcctg ccattactgg ccttacacaa aaaagtacgc
     1081 aactaaaaac agaatctaca ccggatacga aaccgatacg cggaatgcgt accgatcccc
     1141 cgactaaccc attccaaccc ggtaaccagc aggcattaaa acacggtggt tatgcccgtc
     1201 gccttctgct caaagatgag gtgatagagg acgctaaagc gttgacgctc gaggacgaat
     1261 tatttcgcct tcgtgctaac aaccttgtcg ctgcagagaa tattggtcgg tggttggtgt
     1321 cgctggaaga tgctaatggg gaccaggaaa ggaagatgct gattgaaaat atcagcgccg
     1381 ccgagaaagc aatgatgcgc aatacagttc gtattgagtc catcgttggc acgcttgcga
     1441 cggtaggaaa aatatttgct gatacagcct accgcaaggc cgccactgat aaggtgtctc
     1501 tggaggctga tcgtcttcgc cgtgatgcag gtattgatga tggcaatgga gagcgtgacc
     1561 tcaatgactt ctactctgac atccaaaccg acgctgaatc cggctttacg tagtttctgg
     1621 actatgcggg cacgtaacaa agtgctttat ggtggtcggt catcgtcaaa atcatgggat
     1681 gccgctggca ttgccatatt tctgtcgaat aaatacaccc tgcgtttttg ttgtgcccgt
     1741 cagatccaga ataaaatcga agagtcggtg tataccctgc tcaaaattca gatagacagg
     1801 tttggtctgc ggcaccgttt ccgtattctg aacaacaaaa tcattaaccg ggttactggc
     1861 tcggaatttg ttttttatgg attatggcgc aacatcgaag aaattaagtc actggagggg
     1921 atcgatatgt tgtggctgga agaagcccac gcactgacgg aataccagtg gaaaattctg
     1981 gagccaacga tccgtaaaga gggttcggaa tgctggttca tattcaaccc cggacttgtt
     2041 actgatttcg tctggcgcaa cttcgttgtt gatccgcccg aaggcactct catccgcaaa
     2101 attaactatg acgaaaatcc gtttctgtct gacaccatgc ttaaggttat cgacgcggcg
     2161 cgacgccgtg atccggatgg ttttaaacat gtgtatgagg gcgttccgga gtctgatgat
     2221 gatgcggc
//
LOCUS       DAOFMX010000216         2188 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.218, whole genome shotgun sequence.
ACCESSION   DAOFMX010000216 DAOFMX010000000
VERSION     DAOFMX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.218"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..699
                     /locus_tag="QBA20_004347"
     CDS             <1..699
                     /locus_tag="QBA20_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294613.1"
                     /translation="ARAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVE
                     NVVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNG
                     NTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAF
                     NGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIP
                     VLDVSDISVKLQEA"
     gene            701..1306
                     /locus_tag="QBA20_004348"
     CDS             701..1306
                     /locus_tag="QBA20_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HDQ6294614.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1293..>2188
                     /locus_tag="QBA20_004349"
     CDS             1293..>2188
                     /locus_tag="QBA20_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6294615.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          532 a          508 c          611 g          537 t
ORIGIN      
        1 gcccgtgccg gtgtgccggg cagcgatgag gaaacccgtg cggactttga aaaccgccga
       61 cgtaattcag ttgcccgtaa tgcccggaat attctggaag ccatccgggg tgaaatactc
      121 tccacggtag aaaacgtggt ggatgtttac gtcacccata atccgaaaaa aacagaacaa
      181 aaagccggag tcagtcagta tccattaaca cccggttcgc tttatgttgg cgtgtacggc
      241 ggcagcccgg cagatatcgc ggcggccatc tggcgtaagg ctccgccggg tattgatatg
      301 aatggcaaca caacgttcac tgttgcagat aaggagtacg atccgccgta tcctgaatac
      361 gtgatcagct ggcagacact caaacccgtc agtctgcatg tcagtgtgac gctgaaaaaa
      421 agtgactacc tgccttcaga tattacccga caggtacagc aatccgtgct ggacgcattt
      481 aatggtacag atggtggtct gcgggcaaga gttgcctctg ttgtctctgc cgggcgctac
      541 tatgccggca tttacaaaac cgatccggaa catattgata ttctgggcct tactgtgagc
      601 cgtgatggtt cgtcatggac aacggctgtc actttcggga tagatgagat tccggttctg
      661 gatgtgtcgg atatcagtgt gaaactccag gaggcataac gtgcagaatg tggctgccac
      721 tgtgcttgcg cagtatgctg ccagcccccg actcaatgcc ctcattaaca gctttaacgc
      781 agcgctttcc cccgacagtt ttatcagtga tttttatggt cttatctgga acatcgatac
      841 cgcagaaaag tatggtcttg atgtctgggg aaagattgtg ggtgtcagtc gctggctgac
      901 ggtaaaggac gattttaatt acctgggctt cagcgagtcc aggatggaca ccccggtaat
      961 ggatgatccc tgtccgttta atcaggcacc gttttacaac ggaaaatcgg atacccggac
     1021 tgttgacctg tctgatgctg tataccggcg gctgatactg atgaaagcca tgtcgaacat
     1081 tactgactgt tccgttccgg atattaacct gatgctgaga tttatgttcg gaaaaaaacg
     1141 ccgggcttat gttctgaata atggcgggct gaggatgagt tacgtctttg agtccgcgct
     1201 ctcgttggca gaactggcga ttatccagtc gtcgggtgca ctgccatccc cgccgggtgt
     1261 ttatgtttca gtagttttaa aggagtcccg taatgaaggc cagtgataaa ccccgccagc
     1321 tggcggtccc ctttgcgagt accggagata aaaaccgtat cccggacaag gcgacacagc
     1381 agaccagaga gagcggtaat gctgcgtatg attcaggttt tcctccggtg accatgacag
     1441 cggtctcagc gggaggtata ccgccacacg gcaaggattt taacggtctg atgtacgata
     1501 ttaccgcagc aatacggttc gcccaggctg gcggtttgta cacgtataat gccggttttg
     1561 cgggggccat tggtggatat gcaaaaggag ccattctcgc cggagtcgca acaacagcgg
     1621 tctggctgaa taccacggac gataacctga ccgatcctga aggctccgac agtgcgggct
     1681 gggtaaatct tcttgaggat ccgaaaagga tattcctgcg gcagaagaac aatctgtcag
     1741 accttcagaa taaagggacg gcacgggata atcttcaggt ttacagtaaa gagcagtcag
     1801 atcaacgcta tgttcatcgg gaaggcgata aaataaccgg agagctgaaa atccgtggtg
     1861 ttaatgcgct gaggattttc aacgaagctt ttggcctgat ttttcgtcgt tcggaagagt
     1921 gcctgcacct tattcccacc agtgaaggtc agggggaaaa tggcgatatt ggtcccctgc
     1981 gcccgttcac cattaatttg cggacgggtg aaatatccat gtcgcataaa gtgtctgttg
     2041 gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt tcagaacgcc ctggggggga
     2101 attcaattgt tcttggtgat aatgacaccg gatttaaaca gaatggagac ggtattctgg
     2161 atgtttatgc taatagtcag cgtgtatt
//
LOCUS       DAOFMX010000217         2187 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.219, whole genome shotgun sequence.
ACCESSION   DAOFMX010000217 DAOFMX010000000
VERSION     DAOFMX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.219"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(220..384)
                     /locus_tag="QBA20_004350"
     CDS             complement(220..384)
                     /locus_tag="QBA20_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294616.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="QBA20_004351"
     CDS             1286..1570
                     /locus_tag="QBA20_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDQ6294617.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="QBA20_004352"
     CDS             1558..2043
                     /locus_tag="QBA20_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ6294618.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAOFMX010000218         2186 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.220, whole genome shotgun sequence.
ACCESSION   DAOFMX010000218 DAOFMX010000000
VERSION     DAOFMX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.220"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            104..283
                     /locus_tag="QBA20_004353"
     CDS             104..283
                     /locus_tag="QBA20_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294619.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            335..529
                     /locus_tag="QBA20_004354"
     CDS             335..529
                     /locus_tag="QBA20_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294620.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1328..1664)
                     /locus_tag="QBA20_004355"
                     /pseudo
     CDS             complement(1328..1664)
                     /locus_tag="QBA20_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          696 a          446 c          453 g          591 t
ORIGIN      
        1 tttagactat cacttattta agtgatactg gttgtctgga gattcagggg gccagtctag
       61 gtagcgtaag gcactacagc gcattgcttt acgagtaata acaatggatt tatcaccaga
      121 agacgcaaag tttttacgag aactgcaaca ccgtatgctc agagaagcca ttatcgaaca
      181 gcaaacgggc atgagcgcca tagacccatt gcgatacaaa cgggcctcat ctcctttagt
      241 tgacgacaat tccaatgcgg ataaaacacg tccaccacac tgattacatt tttcattagc
      301 acagtggtgt gatgcagcat acgaggaaaa ccggatgaat gagaacaaaa taaaaaggct
      361 ggagcaactg ttacaggcac gccaacggga gtttgccact aaatggggac gtgccgcgct
      421 gccacatgaa cgcctaacct attttccact tgaagacctc attaaaaata atggcaaaaa
      481 aatcaggtct gataagaaac acttcttaga atgcaatgaa gacaaataac aacaatctgc
      541 atattgccac caaccacaga tgtggcatcg ttatcagcgc acttgatttt ctttatattc
      601 actgacgagc ttgccggggc acctttgttt gccggtcatt ctggtatcat gcagatattt
      661 acggcgtaca agcccactag catgacattg taataaggcc tgagcctaac gctttaatgt
      721 gtgtcataca ctcagtggcg taatcgttga caatatggtg catgtaatcg taagcgtcca
      781 gcgaaatcca tctggtcata atctgaagca tccgacaaag tggtgtccac caaataagtg
      841 atctgcgccc aatatcctga gccggaactt tgacaaaggc caacgaaaaa tgggagcgga
      901 tgttacagag ttctcggtac aggggaaaag tggtacctgt cgccagttct ccgatctgtt
      961 taaccgggaa attatctcct acagcttgtc agaaaggtcg gtgatggagc atcgttaata
     1021 cgatgctata cgatgcattc acagtgcccg gccagaggat gccccgtcgc tgcatatgga
     1081 tcagggttgg caatatcgaa tcgcaggcta taggcaaagt taaagcccca tggagtagca
     1141 caaaatatgc cgcacaaagg aaacggtctg aataacgcag tgatgaagaa cttcttcagc
     1201 acactgctaa aacgcatagt gatcgagcgc tgattctggc gaacaactga actaatacat
     1261 cagaatatgc attatgttaa ataaatatga aaagatggtt taaatacccc gttacttttg
     1321 acttacacta tacggtatcg catcgtttaa tattcgcacc ggccagattt ttatttctat
     1381 tagttgtcac aatactgaat gcgtacgacc acagtattct ggctcctgtg tggttatgct
     1441 ttaattctgc gttccgggca gataagcagt tgcttgcagg aatccttctt gtgttaatgt
     1501 cagttcccct ttttaccagt gctgatttcc acattccgtc caacagagct tatagccttt
     1561 ccctggatta tagcattgtc cggctgaagt tctttttgaa taataataga agcactgctg
     1621 gcagatccag tccgtttttc ataacccact gtactgataa ccataatcta atcagtagaa
     1681 attgagtcga aaataagcac tactccatac aggataatta gaggtcagtt tgattattca
     1741 caattcatca tcagcatttt ctatttctga cgaaatcaac atgaaaataa ccatatatga
     1801 taattattat aataacggct ttaattggaa tacatatatt acaacgtatt atatataatt
     1861 ggtattctgg gaactatatt ctcaaaatat agtagaaacg aggtatgttt ctggtggaaa
     1921 ggacagtggg attaaaaagt aagaattgat aaaaaaaacg ccagcaacac atagtgccgg
     1981 cggagggaat accccatgga gaaaatgtga tgcctagagc atcatgaata tataattaaa
     2041 aatagttagc gttgtcaata ctttcacaaa aataattttc gtagtataga aagatatttt
     2101 tatgcatgac ctacctgaat ttgctccggg tagaggttat aaataaaaat tgaatcacga
     2161 caaacacaat attcatagta tggcga
//
LOCUS       DAOFMX010000219         2151 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.221, whole genome shotgun sequence.
ACCESSION   DAOFMX010000219 DAOFMX010000000
VERSION     DAOFMX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.221"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="QBA20_004356"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="QBA20_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HDQ6294621.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="QBA20_004357"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="QBA20_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="QBA20_004358"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="QBA20_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          632 a          395 c          491 g          633 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtgac tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aatccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaattactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgggtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DAOFMX010000220         2126 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.222, whole genome shotgun sequence.
ACCESSION   DAOFMX010000220 DAOFMX010000000
VERSION     DAOFMX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.222"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..241
                     /locus_tag="QBA20_004359"
     CDS             <1..241
                     /locus_tag="QBA20_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294622.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="QBA20_004360"
     CDS             complement(238..1179)
                     /locus_tag="QBA20_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HDQ6294623.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="QBA20_004361"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="QBA20_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..>2126)
                     /locus_tag="QBA20_004362"
     CDS             complement(1684..>2126)
                     /locus_tag="QBA20_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294624.1"
                     /translation="LKTLLKALLAKLERNATGDNDNKPDDNPTGDGEDDEEETTITGD
                     SAWRAEVIVPGIDLSRKMKPTAFKREVLASADKTLVRQIVGDADIRKLPKQSVDMAFN
                     AVSEVAKGRNTRATTGDAQRLNMGMTSIASLNKQNAEFWANRKG"
BASE COUNT          458 a          588 c          545 g          535 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca accatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggt
//
LOCUS       DAOFMX010000221         2096 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.223, whole genome shotgun sequence.
ACCESSION   DAOFMX010000221 DAOFMX010000000
VERSION     DAOFMX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.223"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="QBA20_004363"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="QBA20_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDQ6294625.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="QBA20_004364"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="QBA20_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDQ6294626.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMLILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          560 g          515 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgctcatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tttcgt
//
LOCUS       DAOFMX010000222         2073 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.224, whole genome shotgun sequence.
ACCESSION   DAOFMX010000222 DAOFMX010000000
VERSION     DAOFMX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.224"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..368)
                     /locus_tag="QBA20_004365"
     CDS             complement(<1..368)
                     /locus_tag="QBA20_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDQ6294627.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEA"
     gene            complement(407..694)
                     /locus_tag="QBA20_004366"
     CDS             complement(407..694)
                     /locus_tag="QBA20_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294628.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(944..1099)
                     /gene="hokD"
                     /locus_tag="QBA20_004367"
     CDS             complement(944..1099)
                     /gene="hokD"
                     /locus_tag="QBA20_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDQ6294629.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1327..1992)
                     /locus_tag="QBA20_004368"
     CDS             complement(1327..1992)
                     /locus_tag="QBA20_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDQ6294630.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          532 a          532 c          468 g          541 t
ORIGIN      
        1 gcctcaaacg actttagcaa tgccgtatca cgggttctgc gtgtcgcaac tgtattcaga
       61 tattgctctg cggcttcgct cagagctggc gtatgttccc ggcctactga ttcacacagg
      121 taatcaacga aaccggacac cagttttcgt tcgcgaggcg tgatcgcccc accgaccgga
      181 gtccagtaat cgaaacccag ttgcaggagt ttgaaaaaac gcttgtggaa tgcgtagtta
      241 cgcacacgct taaagtctgc gtgtatccac tcacctattt tgatttgatg cagaaaatcg
      301 caactctccg gcgtcgccgg gagaagtaat ccggaagagg tttgtttgac cagttgtata
      361 tgtgccatcg gctttctccg gtggcacggt gttacacagc aggagttcaa tcctgctcaa
      421 gattgtagat gagtttattc ttcagcaaaa gcagaaaacc agccttaaaa ccaatctttt
      481 tcaaaacccg taatgatgtg acaaattcgt cctcacgcaa aataaaaccg tccgtcagaa
      541 gtccattaca aaaataaaat aacacagcac cgctcttcct ttgttgagat tgcaaacatc
      601 taatgcggca atggctgaca atcgctccat tctcaacgcg cacagcatag aggccatttt
      661 cactaaaaat ttcacgcaat tcttcgattt tcatcttcag aatccttcca gataaatagc
      721 tctcccctgt tcggggtcca tccctcttct ccctgcgcgc tacttaagtg agtcgattct
      781 atctgcgaag gtgcgcgaat caaattcacc ggaaataaac aacaaaaaac ccgccgaaac
      841 gctgagagat cacagcgatg ggcgggttaa gtgcgggtgc gttgaggatg cctgacacat
      901 cagaggtggg cggggatggg atcagctccc cacccggtca ctcttacttc ctggattcgt
      961 agtctacgaa gacagcgacc tccgtctggc cggttcggat tcgtacctca cagaggtctt
     1021 tcctcgttac cagtgccgtc actatgacgg ttaaacagat gacgatcagg gcgattaaca
     1081 tcgccttttg ctgcttcata gcctgcttct ccttgccttt cggcatgtaa gaggctaacc
     1141 tacatgtgca aagcatgaaa ttggcctcag attaatgtta agcgtcttgc cggacgcgta
     1201 atgttaactg gggcttttct ctatctgcct tttggtgttc atgcctgaag cagatagcct
     1261 caagcacccg cagtcattct acttacctcg ccaatatgaa atcaatcaga aaggtgcccc
     1321 ataaaatcac tccttctctt ctttaccgta gtggagttga ccaattttga taagagggcg
     1381 tccctgagat ttgcggtgta gattggtatc gcgcagagaa tacacacagc cacaatattc
     1441 ctgctgatag aatttttcgc gcttgctgat ttcaatcata cgggacgagc cgccctgctt
     1501 gcgccagtta taatcccagt acaccatgcc cggataatgc gcgacggctc gccgcccaca
     1561 gtcgttaacc tgctgcatat ttttccagcg tgaaatgccc agtgaactgc tgatcacact
     1621 gaaaccattt tcagcggcgt acagcgctgt ccgctcaaaa cgcatgtcaa aacacatggt
     1681 gcaacggatc cctcgttcgg gctcccattc cattcctttg gctcgttcaa accagttgtc
     1741 tgtgtcgtaa tcagcatcaa taaacggcac gccgtgttgt tcagcaaagc gaatattctc
     1801 atccttacga attaaatact ctttctgagg atgaatgttc gggttgtaga aaaagatggt
     1861 gtagtcgatt cccgaggcct gaagcgcctc catcacctca ccggaacatg gagcacagca
     1921 agagtgcagt agtagtttgt ttgccccgtt tgggagctcc aatttgggcc gtttgaaatc
     1981 agcaactgtc ataaatatgt ttattgaggt cataaaaata tcaaagagtg tagcattaga
     2041 acggggctat cggaaacaga tgtgtgactg ctc
//
LOCUS       DAOFMX010000223         2033 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.225, whole genome shotgun sequence.
ACCESSION   DAOFMX010000223 DAOFMX010000000
VERSION     DAOFMX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.225"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..363)
                     /locus_tag="QBA20_004369"
     CDS             complement(<1..363)
                     /locus_tag="QBA20_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6294631.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="QBA20_004370"
     CDS             complement(430..1644)
                     /locus_tag="QBA20_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HDQ6294632.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTLIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          635 a          494 c          411 g          493 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcag cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcca ccgaggagaa aaaataccct caaaaaccac ctgaataatt
     1801 aaccattatt gttaaaacaa cacgttaaat aatcacataa caaactccat taatcattga
     1861 aaatgagcac agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat
     1921 gtatttaaaa atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa
     1981 ggaatgtgac aaataatggt tctggttggg cgttaaattt aaggtcacca gaa
//
LOCUS       DAOFMX010000224         2032 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.226, whole genome shotgun sequence.
ACCESSION   DAOFMX010000224 DAOFMX010000000
VERSION     DAOFMX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.226"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..>2032
                     /gene="sat"
                     /locus_tag="QBA20_004371"
     CDS             <1..>2032
                     /gene="sat"
                     /locus_tag="QBA20_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HDQ6294633.1"
                     /translation="YGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGI
                     ASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQE
                     KITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKESI
                     VNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKV
                     ILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLA
                     INNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQGH
                     ATEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDWK
                     TGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFD
                     FKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIA
                     QNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVT
                     GNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNA
                     IWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQA"
BASE COUNT          745 a          359 c          400 g          528 t
ORIGIN      
        1 catacggcat gatgatttat aaaaatgata aaacatttcg taatcttgaa atattcggag
       61 acagcggctc tggagcatac ttatatgata acaaactaga aaaatgggta ttagtcggaa
      121 caacccatgg tattgccagc gttaatggtg accaactgac atggataaca aaatacaatg
      181 ataaactggt tagtgagtta aaagatacct atagtcataa aataaatctg aatggcaata
      241 atgtaaccat taaaaacaca gatataacat tacaccaaaa caatgcagat accactggta
      301 ctcaagaaaa aataactaaa gacaaagata ttgtgttcac aaatggggga aatgttctgt
      361 ttaaggataa tttggatttt ggtagcggtg gtattatctt tgacgaaggc catgaatata
      421 acataaacgg tcagggattt acatttaaag gagcaggaat tgatatcgga aaagaaagca
      481 ttgtaaactg gaatgcattg tattccagtg atgatgtttt acacaaaata ggccccggta
      541 ctctgaatgt tcaaaaaaaa cagggggcaa atataaagat aggtgaagga aatgttattc
      601 ttaatgaaga aggaacattt aacaatatat accttgcaag cggaaatggt aaggtaatac
      661 taaataaaga taattccctt ggcaatgatc aatatgcggg gatatttttt actaaacgtg
      721 gtggcacgct agatttaaat ggacacaatc agacttttac tagaattgcc gccactgacg
      781 atggaacaac aataactaac tcagatacaa cgaaagaagc cgttctggca atcaataacg
      841 aagactccta catatatcat gggaacataa atggcaatat aaaactaacg cacaatatta
      901 attctcagga taagaaaact aatgcaaaat taattctgga tggtagtgtc aacacaaaaa
      961 atgatgttga agtcagtaat gccagtctta ccatgcaagg ccatgcaaca gagcatgcaa
     1021 tattcagaag cacagcgagt cattgctccc tggtatttct ttgtggaacg gactgggtca
     1081 ccgttttgaa agaaacagag agttcatata ataaaaagtt caattctgat tacaaaagta
     1141 ataatcagca gacctcattt gatcagcctg actggaaaac cggggtgttt aaatttgata
     1201 cattacacct gaacaatgct gacttttcaa tatcacgcaa tgccaatgtt gaaggaaata
     1261 tatcagcaaa taaatcagct atcacaatcg gcgataaaaa tgcttacatt gataatcttg
     1321 cagggaaaaa tattactaat aatggttttg acttcaaaca aactatcagt actaatctat
     1381 ccataggaga aactaaattt acaggtggca tcactgcaca taacagccaa atagccatag
     1441 gtgatcaagc tgtagttaca cttaatggtg caacctttct gaataatact cctataagta
     1501 tagataaagg agcaaaagtt atagcacaaa attccatgtt cacaacaaaa ggtattgata
     1561 tctccggtga actgactatg atgggaatcc ctgaacagaa tagtaaaact gtaacgccgg
     1621 gtctccacta cgctgctgat ggattcaggc tgagtggtgg aaatgcaaat ttcattgcca
     1681 gaaatatggc atctgtcacc ggaaatattt atgctgatga tgcagcaacc attactctgg
     1741 gacagcctga aactgaaaca ccgactatat cgtctgctta tcaggcatgg gcagagactc
     1801 ttttgtatgg ctttgatacc gcctatcgag gcgcaataac agcccccaaa gctacagtta
     1861 gcatgaataa tgcgatctgg catctaaata gccagtcatc aattaatcgt ctagaaacaa
     1921 aagacagtat ggtgcgtttt actggtgaca atgggaagtt tacaaccctt acagtggaca
     1981 accttactat agatgacagt gcatttgtgc tgcgtgcaaa tctggcccaa gc
//
LOCUS       DAOFMX010000225         2029 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.227, whole genome shotgun sequence.
ACCESSION   DAOFMX010000225 DAOFMX010000000
VERSION     DAOFMX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.227"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..1824)
                     /locus_tag="QBA20_004372"
     CDS             complement(<1..1824)
                     /locus_tag="QBA20_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDQ6294634.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="QBA20_004373"
     CDS             complement(1932..>2029)
                     /locus_tag="QBA20_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6294635.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          484 a          579 c          431 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccgccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAOFMX010000226         1987 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.228, whole genome shotgun sequence.
ACCESSION   DAOFMX010000226 DAOFMX010000000
VERSION     DAOFMX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.228"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            484..627
                     /locus_tag="QBA20_004374"
                     /pseudo
     CDS             484..627
                     /locus_tag="QBA20_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            774..1040
                     /gene="ospE1"
                     /locus_tag="QBA20_004375"
     CDS             774..1040
                     /gene="ospE1"
                     /locus_tag="QBA20_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HDQ6294636.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1431..>1987
                     /locus_tag="QBA20_004376"
     CDS             1431..>1987
                     /locus_tag="QBA20_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HDQ6294637.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          666 a          418 c          338 g          565 t
ORIGIN      
        1 ctctcgtctg gggaatatct ccccggcagc cttcaggata aaatattatc agatgactgc
       61 ttagaaaaag aacaaatggt ggtgtccgct attgccagta cacctcaagc ttcttaccat
      121 atttgaaata aagctcaata gcgcgaattt tttgttcttc agtaaacatg tttcatcttc
      181 tgagagtcca ggaaatcgtc cgcatcccca ttaccgtctt atatcactgg cgctgacatg
      241 tgctcattga tgaagtgaat ctgaatgaca tgttttgcca tatcaacgcc aaccggtgta
      301 tatttcattt gtggagtctc cagtctgagg acgctttatg catcccgtat tgggcagtat
      361 gatgccggaa agctgcgagg ctccacacct gccagtttca cccttttaac catcatacta
      421 ctccgtgttt aattgtgtgg gatgcgttca ttacatttta acagtgtcac cccgtcggcc
      481 acgaaagata aacaggtgac cagaaaaggg atcttcatcc agcacatgct atatcttgaa
      541 cgtaacaacc tgaccctccg cacccgcatc aagcggctgg cacataaaac aatctgcttc
      601 tcgcgttccg tggaaatcca cgaaaagtca tcggctcctt cattgaaaaa cacatattct
      661 actgattgga agcgccaccc gaaaaagtat cgttttttta cagtaaactt catttagccg
      721 actataatgt aaaaaaataa attttttgcc aaaatataac ataaggaagt aatatgctta
      781 cacaaactat attcccttgt cttccacaaa aacaagaaaa tatcatcctt gaagtatcaa
      841 atcctgtatt attatcttct actgttacta cagatggata tactgttttc aataaaaaag
      901 cagctatata cgaattacaa atacctgctg ccagccggac gaaaacgttg aaattcactg
      961 ctacagaaat gcaatggttg acaaagataa atgaagccgg aattgatgaa aaacaaagcc
     1021 aacggtattc tgatttctga agttaatttc agactcgttt aagctccatt ttcaaaaaaa
     1081 atatatcagg gtttgttact aataacttct gaaaacagaa aaaaaataga gcagtaattc
     1141 tatttcagag aggagtaact ccatctatcc tcgatacgta ctgaacgtta tcgtgatata
     1201 ttcatcatgc tttcttcacc attattcgag tatagggaga gacgcaatga ccttaaatac
     1261 ctttttatgg ctgaattacc attctcagga ataacagcag actccttccc atagacggct
     1321 tagggggaaa gcaatacccg gagaaagggt acaaagcaaa ctgttttaaa attaataagc
     1381 tatattctat ttaactagaa ttcttatcgt tgataagaat tctagttata atgattaaat
     1441 caaccaatat acaggcaatc ggttctggaa ttatgcatca aataaacaat gtatactcgt
     1501 taactccatt atctttacct atggaactga ctccatcttg taatgaattt tatttaaaaa
     1561 cctggagcga atgggaaaag aacggtaccc caggcgagca acgcaatatc gccttcaata
     1621 ggctgaaaat atgtttacaa aatcaagagg cagaattaaa tttatctgag ctagatttaa
     1681 aaacattacc agatttaccg cctcagataa caacactgga aataagaaaa aacctattaa
     1741 cacatctccc tgatttacca ccaatgctta aggtaataca tgctcaattt aatcaactgg
     1801 aaagcttacc tgccttaccc gagacgttag aagagcttaa tgcgggtgat aacaagataa
     1861 aagaattacc atttcttcct gaaaatctaa ctcatttacg ggttcataat aaccgattgc
     1921 atattctgcc actattgcca ccggaactaa aattactggt agtttctgga aacagattag
     1981 acagcat
//
LOCUS       DAOFMX010000227         1964 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.229, whole genome shotgun sequence.
ACCESSION   DAOFMX010000227 DAOFMX010000000
VERSION     DAOFMX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.229"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="QBA20_004377"
     CDS             complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="QBA20_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HDQ6294638.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          622 a          333 c          311 g          698 t
ORIGIN      
        1 tcaccacatg caatgtgttg ttgcagggga acggtctgca ccatccccta tatcgatcaa
       61 tataacaaaa atgacaacgt cagattcagg gcacaaccta aatatatttt aggtcacctc
      121 ccaaatcgtt tgcctgatac cgctccattt tttaataaaa aatcaattgt ttttgaatct
      181 ttgcttttca ttgcattgtc aagcatagtg tccccactat ttgctttttg aaatcttttg
      241 tttacatcaa ccaatccatt actaataaaa tattctagca ctttatagtt ggcactatct
      301 tcactaagta aatactcaac atcatacagc tcacaataca tattgttgtt cattttctcc
      361 atctctgcga catcttgttt tgtaaaatta aatttagata gtaaataaag tgccatatca
      421 gattttgcat tgcctagaga ataagcaaga gcttgatgag ccattttttt atcagtcacc
      481 ttattgttaa tttcttcata attggatgct ttaatggcat cctccaaaga tatatctctg
      541 agtcttatct ttttgaagtt atctgtgctt agcattcttg gtatatgata ctcaagctga
      601 aacacaaagt ttataattct atccagacta acaggatcaa tgtttttatt ataacaaaac
      661 tctcggaatc tttgatcttt actctttctt ataaaatcaa taagatgcaa cccaattcca
      721 agcctcatat ctttagtatt aaatattggc acatcagttt ttccctgatt accatgcaca
      781 tacctgctaa cttcatccac aacctcttta aagttatcta agaaaaatga ttggtgctca
      841 tggtaaaaaa caggtggaat agcattatca aagtggtcgg ttaatgtcat atatccatat
      901 ggagagtcat gatcaatgat atacgcatta gcaccaaaat ccacagtatg atgtgtttta
      961 tttagtggga gtgtttcttg atgattggaa atttcaaccc caaaaaaaac aaaatcgtgg
     1021 ttagctattt cttcaatatc ctcagagaat gtgtttttat atgtttgtat attctcagac
     1081 ttaattttat caaaggactt aatattaaga gtattatttg acaggttagc gttactttga
     1141 tgtctgaagt taaaatcaga attaataata atagagagca agtgacgttc atccgaagaa
     1201 agaggatgag ttttttgtat tatttcagaa agatagtgtc tggacacctt atcgaggtgc
     1261 tcaagtgacc gaaatgatga ccgtgctcca gatggcgctt caatagaaag atttaatgat
     1321 ttgaaactgg tcccttgaga gaacatttta ctataacttt gcgctgctat agacttcctt
     1381 aaaaagcttt tcacagtatc agcacagtga tctaaatgtt tttcacttat gccagaagag
     1441 gatgagtttg ttacgcatga tatgtttttt tgtaatgctt ttgtatcttt gttcggattt
     1501 atttttccat caacaagatc aatattgttc tgaacattaa tatgatttac tgcttcaggt
     1561 attttcataa aacatccccc attatgtgcg tttttatctg cagttattta gaataatatt
     1621 atctaactta atattttctt gtatgtatta taacgaacat attttaatat gttgtacgat
     1681 tttcactctg aaatcccctg agtttaatca cacttccttt actgaaagga attattgcaa
     1741 cagtattatt tgtttttgga tatatatctt gcgccttgtt atgtagttaa tgctcagaat
     1801 atgatagata aaatagagac gcaaaacagg gcatgctcat aacatgatga taacataaaa
     1861 aattagtaac aataaatgaa agaatcatgg gcgctcaaca aaaagagggt aatgctgcca
     1921 acttactgat ttagtgtatg atggtgattt taaggtgctt gcgt
//
LOCUS       DAOFMX010000228         1938 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.230, whole genome shotgun sequence.
ACCESSION   DAOFMX010000228 DAOFMX010000000
VERSION     DAOFMX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.230"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="QBA20_004378"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="QBA20_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HDQ6294639.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1938
                     /locus_tag="QBA20_004379"
     CDS             1494..>1938
                     /locus_tag="QBA20_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDQ6294640.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDD"
BASE COUNT          552 a          336 c          467 g          583 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgct ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
     1741 tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat ggcggcgact
     1801 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
     1861 agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt attgctcatg
     1921 ccgattatcc agatgatg
//
LOCUS       DAOFMX010000229         1921 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.231, whole genome shotgun sequence.
ACCESSION   DAOFMX010000229 DAOFMX010000000
VERSION     DAOFMX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.231"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            193..462
                     /locus_tag="QBA20_004380"
                     /pseudo
     CDS             193..462
                     /locus_tag="QBA20_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            459..685
                     /locus_tag="QBA20_004381"
                     /pseudo
     CDS             459..685
                     /locus_tag="QBA20_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="QBA20_004382"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="QBA20_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HDQ6294641.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1643..1921
                     /locus_tag="QBA20_004383"
                     /pseudo
     CDS             1643..1921
                     /locus_tag="QBA20_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          540 a          417 c          458 g          506 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgactaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat gacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgaaagtt tattcccaat agattttcgc aatatttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagacgtat ttgttgatgc
     1321 tataagccag aggctcttct atgagaatat ggggaccatt ccctcgcgat attaacgtaa
     1381 tatcttaatt gtatgcaccg ccagaatgcg ttcagtgcga tcacggcagc agacaggtaa
     1441 aaatagcaac aaaccacccg aaaaaccgcc gcgatcgcgt atgataaatt ttaaccacat
     1501 gcatacctgt gcagcaaggc gaatcacgca cggatttcca gccagagaca cctgaaacgt
     1561 aaccggctca tttaaaccgt ctggtctgtt acctccggtt ccacaaaaat aatgtccatc
     1621 attttgaagt ggacactatc gtatggaata ccggacctga atcactgaag ctttacgcct
     1681 tcatttcgaa gaacatttac cacgggttgt ggccggatgt cgcctgggcg taccaaaatc
     1741 aactgtctgt ggtatgttcg tgcgcttccg caacgctggc ctttcatggc ctctgcccgc
     1801 aggtatgtcg gagcaggaac ttgatgccct tctttacgga agtgcctcca cagtacctgt
     1861 cgtacttact gaaagcaccg ttatgcccaa actcccggtg gtaaaaaaac gtccccggcg
     1921 g
//
LOCUS       DAOFMX010000230         1913 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.232, whole genome shotgun sequence.
ACCESSION   DAOFMX010000230 DAOFMX010000000
VERSION     DAOFMX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.232"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(53..1702)
                     /locus_tag="QBA20_004384"
     CDS             complement(53..1702)
                     /locus_tag="QBA20_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDQ6294642.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAOFMX010000231         1880 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.233, whole genome shotgun sequence.
ACCESSION   DAOFMX010000231 DAOFMX010000000
VERSION     DAOFMX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.233"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(1..279)
                     /locus_tag="QBA20_004385"
     CDS             complement(1..279)
                     /locus_tag="QBA20_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294643.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="QBA20_004386"
     CDS             220..405
                     /locus_tag="QBA20_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294644.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="QBA20_004387"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="QBA20_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDQ6294645.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="QBA20_004388"
     CDS             complement(1254..1670)
                     /locus_tag="QBA20_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294646.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="QBA20_004389"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="QBA20_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
BASE COUNT          475 a          468 c          410 g          527 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc
//
LOCUS       DAOFMX010000232         1846 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.234, whole genome shotgun sequence.
ACCESSION   DAOFMX010000232 DAOFMX010000000
VERSION     DAOFMX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.234"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..254)
                     /locus_tag="QBA20_004390"
     CDS             complement(<1..254)
                     /locus_tag="QBA20_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294647.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFS"
     gene            279..518
                     /gene="relB"
                     /locus_tag="QBA20_004391"
     CDS             279..518
                     /gene="relB"
                     /locus_tag="QBA20_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HDQ6294648.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            518..805
                     /gene="relE"
                     /locus_tag="QBA20_004392"
     CDS             518..805
                     /gene="relE"
                     /locus_tag="QBA20_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HDQ6294649.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            877..1032
                     /gene="hokD"
                     /locus_tag="QBA20_004393"
     CDS             877..1032
                     /gene="hokD"
                     /locus_tag="QBA20_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDQ6294650.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1250..1501
                     /locus_tag="QBA20_004394"
     CDS             1250..1501
                     /locus_tag="QBA20_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294651.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1568..1846
                     /locus_tag="QBA20_004395"
                     /pseudo
     CDS             1568..1846
                     /locus_tag="QBA20_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          409 c          467 g          463 t
ORIGIN      
        1 atgctaaagc aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca
       61 aagtatatgg tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct
      121 gcacaataca ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc
      181 gttcttacga ctactttctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat
      241 acttgtaatg acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta
      301 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
      361 ctgaagcgct tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac
      421 agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta
      481 atcctaagcc agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga
      541 cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa
      601 gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc
      661 tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga
      721 cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag
      781 cgaggcggtc aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt
      841 gacacttctg ctttgcgttg acaggagaag caggctatga agcagcaaaa ggcgatgtta
      901 atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac gaggaaagac
      961 ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac agcttacgaa
     1021 cctgaggagt aagagacccg gcgagtgaga aatccctcgc cacctctgat gtggcaggca
     1081 tcctcaacgc acccgcacta aacccgcttc ggcgggtttt tgtttttatt ttcaacgcat
     1141 ttgaagttct ggatggtgcc ggaatagaat caaaaatact taagtagcgc gcagggataa
     1201 aagggatgga ccccgaacag gggagtgcta cttatctgga aggattctga tgatgaacat
     1261 cgaagaactg cgtaaaattt tttgtgaaga tggcctctat gctgtgtgcg ttgaaaatgg
     1321 aaatattgtt agtcattacc gcattgtgtg tttgcaaaaa aatggggctg cgttaattaa
     1381 ttttgtggat gcccgagtga cggacggatt tatcttgcgc gacggtgagt ttgtcacttc
     1441 attacaggca ttgaaagaga tcggaataaa agctggcttt tctgcttttt cagaagaata
     1501 aactcatcta caatcttgcg cggggctgaa ctcccgctga gtaacaccgt gccaccggag
     1561 aaaaccgatg gcacgcaacg caaaatatta caatcatgat aattcgaccg ttcttgccca
     1621 cacgcacgag cggtattctc acgcatttaa gtcagactgg taccagcatc cctcatgcac
     1681 tgaagaacag gccgaatggc ttattcagtg ttaccgcagg cgcggatgcg aggttaaaaa
     1741 agcccttagc ctcgactacc gtcactagat aatctccgtc aggctccctt actccgaaca
     1801 gccaccgcgt ccgtcccgca cattccagca acggatctgg aggtaa
//
LOCUS       DAOFMX010000233         1834 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.235, whole genome shotgun sequence.
ACCESSION   DAOFMX010000233 DAOFMX010000000
VERSION     DAOFMX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.235"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..181)
                     /locus_tag="QBA20_004396"
     CDS             complement(<1..181)
                     /locus_tag="QBA20_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDQ6294652.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(322..876)
                     /locus_tag="QBA20_004397"
     CDS             complement(322..876)
                     /locus_tag="QBA20_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HDQ6294653.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(873..1163)
                     /locus_tag="QBA20_004398"
     CDS             complement(873..1163)
                     /locus_tag="QBA20_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDQ6294654.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1163..1762)
                     /locus_tag="QBA20_004399"
     CDS             complement(1163..1762)
                     /locus_tag="QBA20_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDQ6294655.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          497 c          422 g          470 t
ORIGIN      
        1 ggttatgggt gatcggggag agagtttcaa cagagatttg agtggtcata acgataactc
       61 cgtacatttg gaaattatcg ccaccgacga cgccaatcga actggtggcg aactgtgcag
      121 ggttggcgta accgggtacg gaaaccggcg agcctttcgg ctcccccaca cagcccgcca
      181 taaatcgcga atgtgactgt gcaaacgata tgaaaaaaga cgcgggcgcg tctcatatcg
      241 ctccgtaaac atccgggacg ccaatcccga cgccagattt tgctggcgcg tgaggaatat
      301 agccccggat aacagattga gtcaacaaac ggtttttaga tccccggaag agaatgcatc
      361 acgcatcggt agatagagca tatattcagc aagatttagc catacatcta cccgactgca
      421 gcacgtccgg aaacaccact cagggtgtgc gtcattcagc aattcagcca ttttacgctt
      481 agtcatcccc cgcccttcat accgttgccg gaggacacta atcaatccag gatgctctgc
      541 cagcacctca cttatgactc tatcaataca taacgcctct gcatcagtac aatgcgccag
      601 ccaggtcttt tgcttgccat tgatcatctc tcgcaaaaac gcttccagct caggtttctc
      661 tattcccgct tttttcatcc tgcgcagggc ttcattgacg gctgttttcg tcaatttttt
      721 ggatgccagc aactggttaa acatatttcc cgtcttaccg ccgccaatat acgaccagcg
      781 cccccacatg cgcagttttc cctgaatcca gacactttcc agcgtggtga gacgaaggtg
      841 ttccccgctt ttgcctgtat ttgttgggta aatcataaat aaccttcctt tctccagatt
      901 tcttgtgtgc gaaaaacacc ttctgcatgc atcaggcgta attcttcttt ggtgtaatcg
      961 ctggttttta cccgcccgtc gattaaatcg tggcatgagc tacaggcaat cgctgcctgc
     1021 atatcgtgtg gttttgtcgc tgttccgcac gtccccgcca gcctgtaatg cgccagcaca
     1081 gaagtttcgg gattgtgatt gcagtagcca ggaattctga cggtgcacat ctgcccccgc
     1141 gccgctttac gtaaatccac cattacgcaa actccagtaa ctgcgcggcc acattttcga
     1201 cttcctccgg agaggaaaat ttacggaaca ggatccagtt ccacagcaca ttcagtacag
     1261 atttataaac ctgctgaaac tcggtttcgt ccatgttcgc aaatgcgata gattttgccc
     1321 tgcgcccacg actaccatca ggataaatat gctcggtgta aaatccggcc tgaatggtta
     1381 cccactcgcg gaaagcgtca aacgacttta gcaatgccgt atcccgggtt ctgcatgtcg
     1441 caactgtatt aaggtattgc tctgcggcat cactcagggc tggagtgtgt tcccgaccta
     1501 ctgattcgca caggtaatca acgaagcctg ataccagttt tcgttcgcga ggcgtgatcg
     1561 ccccaccgac cggagtccag taatcaaatc ccagttgcag gagtttgaaa aaacgcttgt
     1621 ggaatgcgta gttacgcaca cgcttaaagt ctgcgtgtat ccactcacct attttgattt
     1681 gatgcaaaaa atcgcaactc tccggcgtcg ccgggagaag taatccggaa gaggtttgtt
     1741 tgaccagttg tatatgcgcc atcgtagttc tccgctggcg cagtagaatg ggtgttcagc
     1801 ccgttatgta gtataccaga attaatgcca atac
//
LOCUS       DAOFMX010000234         1821 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.236, whole genome shotgun sequence.
ACCESSION   DAOFMX010000234 DAOFMX010000000
VERSION     DAOFMX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.236"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(1..887)
                     /locus_tag="QBA20_004400"
                     /pseudo
     CDS             complement(1..887)
                     /locus_tag="QBA20_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            895..1401
                     /gene="tnpA"
                     /locus_tag="QBA20_004401"
                     /pseudo
     CDS             895..1401
                     /gene="tnpA"
                     /locus_tag="QBA20_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1419..>1821)
                     /locus_tag="QBA20_004402"
     CDS             complement(1419..>1821)
                     /locus_tag="QBA20_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDQ6294656.1"
                     /translation="LDKILNYTNTEMKEAIAQRKPVVTTAGPRSLIFRGAITGVDTSK
                     EGLQFYEVVPVALVVAGTQMATGHRTMDTRLYFEGELIDAATNKPVIKVVRQGEGKDL
                     NNESTPMAFENIKQVIDDMATDATMFDVNKK"
BASE COUNT          431 a          454 c          426 g          510 t
ORIGIN      
        1 tttaaacctg attttgcccg gacattgcgc cccggctggc tgaccgtacc cgctgctgac
       61 tttgacatgt tgctgacctt caaatcctca atgactatca ttgcgtggtt tttgctgacg
      121 gccgttgtga ctttgtgaag gtagtccctg cggatatttg cgatacagga atgcagtcgc
      181 tgtattttgc gtttctgctt ctgccagttg ttgctgaatt tgaccttgcg gcttaactgt
      241 cgctgaagtc tcgccagcgt cttctggttt ttctgaaaac tgtttacagg ctcaaagact
      301 gtgccatctg acagcgtggc gagtttagcc acgccagcat ccagcccgac cattgatgct
      361 gaagggtgag ccggagtgga tacttcactt tctgtctgaa tactgatgta ccacgtaccg
      421 caggactggc tgacagtgac atttttcaca acacccgtga cctgacggct attccggtag
      481 cgcatccagc ccagtttcgg cagaaaaata cggctgtttt cctgatcgag cttaacgccc
      541 tgcgggtagc ggaatgcatc attctgtcac cgctttttga atcggggaaa agcagcccgc
      601 ttccggaaga agtttttgta agcccgctca aggtctttca gtgactgttg caatggctgt
      661 gagggagaat ctttaagcca ttgcgtttca gtggcatttt tccactcaac cagccaggaa
      721 gccattttcc cgtaagggat gtatttatta ccggcctcat gattctcatt ctgacgtgcc
      781 agagcacgat tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc acactcctgt
      841 tgaccacctg gtcttaactg gaatttaaat gcctgtaatc gtttcacagg tcaaatgata
      901 ctttggtctg tgaaaaaaga aaccgatatc cggcgtggaa ggcattgtgt tttcctgatg
      961 catgttcacc tagtctttgt caccagatac cgacgccaga tttttgacca tgacgcgaca
     1021 gaaaaactac gcacttactt ttcaaatgta tgtgcttatt ttgaagctga actggttgaa
     1081 atggatggcg aaccagatca cgtccatttg ttaattaact aacctcccaa actggcgata
     1141 tccagtctgg taaacagcct caaaggcgta tcgggtaggt tactgcgacg agatcgacca
     1201 gatattgcag tcaggtatta ctacaaaggc gttttgtgga gtcctggcta ttttgccagt
     1261 agctgcggag gtgcgccaat atccgtcatc cgccaataca ttgaacaaca gcaaacacct
     1321 ggtcaggtgg aaaaccgcgc cttatatccc cgccctgaag gatggggttt tacggcgcac
     1381 cggataaaaa gcgccatcta acgatggcgc gtctggaact actttttgtt aacgtcaaac
     1441 atggtggcat cggtcgccat gtcatcaata acttgtttaa tattttcgaa agccattggc
     1501 gtactttcgt tattcaggtc tttaccttcg ccctgacgaa cgactttgat aaccggttta
     1561 ttagtcgctg catcaatcag ctcaccttca aaatagaggc gagtatccat ggtacggtgg
     1621 cctgtagcca tttgcgtccc cgcaaccact aatgcaacag gaaccacttc atagaattgc
     1681 agcccttctt tgctggtatc tacaccggta atggccccac ggaaaatcag actacgcggc
     1741 ccagcggtgg taaccactgg tttacgctgc gctatcgctt ctttcatttc ggtgttggta
     1801 tagttcaaaa ttttatccag a
//
LOCUS       DAOFMX010000235         1809 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.237, whole genome shotgun sequence.
ACCESSION   DAOFMX010000235 DAOFMX010000000
VERSION     DAOFMX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.237"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            173..1198
                     /locus_tag="QBA20_004403"
     CDS             173..1198
                     /locus_tag="QBA20_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDQ6294657.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="QBA20_004404"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="QBA20_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          420 c          462 g          506 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctagct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgtc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAOFMX010000236         1782 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.238, whole genome shotgun sequence.
ACCESSION   DAOFMX010000236 DAOFMX010000000
VERSION     DAOFMX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.238"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            258..1082
                     /locus_tag="QBA20_004405"
     CDS             258..1082
                     /locus_tag="QBA20_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294658.1"
                     /translation="MVDHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLEL
                     KPGILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARR
                     RYGIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKP
                     TGKMDVKGSGAITGLTDKLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLML
                     EKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
     gene            1157..1435
                     /locus_tag="QBA20_004406"
     CDS             1157..1435
                     /locus_tag="QBA20_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294659.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            1432..>1782
                     /locus_tag="QBA20_004407"
     CDS             1432..>1782
                     /locus_tag="QBA20_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HDQ6294660.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIE"
BASE COUNT          532 a          381 c          487 g          382 t
ORIGIN      
        1 atggggttgt tcaactggtt aactcactcg gcgggtcatg ccggacgcga gttaaaactg
       61 gcgtgacata cacatacaag gacgataagc ggcatgggat ggattcatac gaggcaagaa
      121 tcagactgac aagagaaatc agggaggcca tccgttcacc acgactcaat ggcagattaa
      181 ctgcgcatcg attcgagggc tgtggggtat tcgtcaggaa tgttgaaaaa atcggcaatg
      241 cagaatgctt gtgtattatg gtcgatcacc ctcgccacct gtatgtaacc aggggatatg
      301 tggcgacgca taacaccgag gttgtcgggc atatggcact tgaggcaatg cgtcagggtg
      361 tgaagacgtg catcgcgtca cttgagctga agcctggtat tctccttaag cgacttaccc
      421 gtcaggcaac gtgctgcaag atgccgccag tgctggaaat tgactctgca tttaaatttt
      481 atgacgaaag actttgggtg tttggcctga ctggaacggc gaaagccgac aggctgatcg
      541 aaatattcga ctacgctcgc cgccgatacg ggatccagtt attcatcatc gacagcctga
      601 tgaaatgtgg cataggcgac gatgactata acgggcagaa ggcgtttgtt gactcgattt
      661 gcgacttcaa aaacaaaaca aactcccacg tcattctcgt tactcactcg cgaaaaggag
      721 acagcgaaga aaaaccaacc gggaaaatgg acgtaaaagg ctctggagcg ataacaggcc
      781 tgacagacaa acttttcatc atctggcgta acaaggctcg cgagagagcg ttacagagag
      841 ttcagagtgg tgaaaagatg tcagagaagg acgaacagct actggcatct ccggcatctg
      901 ttttgatgct tgaaaaacaa cgtaacggcg aaggttggga aggtggtgtt ccgttgttcc
      961 ttgacgagca atcgcaccag ttcctgcaac ttgaatcagg atcgccatat agctacatcg
     1021 ccaatatgcc gaaatcggaa tatgacgagg cgtggcgaca ggaaaacgtg acggagtatt
     1081 aaatgaccat ctacatcact gagctaatag caggcctgct ggtaatcgca ggccttttta
     1141 tttgggggag agggaagtga acgatagcta ccgacagttt gaaaactggt ggtcaaaaga
     1201 caaaagccag ttcacaggag acgatgaatt aaaagagttt gcttgggtga tatggcaggc
     1261 atcgcgagca gctattgaac tggatatcga ctggcccgaa tcgaatgacg acttttggaa
     1321 agatggtgaa gaaggtgctt atgcgatggg gcatgaggat gggaaggaca aaacggtaat
     1381 tgcagtaatg aaagctatca gagccgctgg aattaaagag aagaattttc gatgaagcaa
     1441 acaatcttcc tccgaactaa gcaacaacag caagccgcaa taaatgccat cctctcaact
     1501 cctctcgata aagacaagcc agtcaccatc cgcattactg actacaagcg aaatcttgac
     1561 cagaacgcaa aatttcacgc gatgctggcg gatatcgctc gtcaggttca atggtgcggc
     1621 aaatggttaa aaccagaaca atggaaggtt ttgttgatca gcggtcatgc agtggcaaca
     1681 aaacaggaag ctgatgtttt gcccggcctt gaaggagaat gcgtcaacat tcgcgaaagc
     1741 agcgcgcaga tgagcgtgaa gcgcatggca agtctgatcg ag
//
LOCUS       DAOFMX010000237         1759 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.239, whole genome shotgun sequence.
ACCESSION   DAOFMX010000237 DAOFMX010000000
VERSION     DAOFMX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.239"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(1..263)
                     /locus_tag="QBA20_004408"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="QBA20_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="QBA20_004409"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="QBA20_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HDQ6294661.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1645)
                     /locus_tag="QBA20_004410"
                     /pseudo
     CDS             complement(1175..1645)
                     /locus_tag="QBA20_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          500 a          435 c          319 g          505 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatctt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacgc ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgaggttc
     1741 ttttcgccag tcctgtaat
//
LOCUS       DAOFMX010000238         1749 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.240, whole genome shotgun sequence.
ACCESSION   DAOFMX010000238 DAOFMX010000000
VERSION     DAOFMX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.240"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            454..1302
                     /locus_tag="QBA20_004411"
     CDS             454..1302
                     /locus_tag="QBA20_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDQ6294662.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1319..1600
                     /locus_tag="QBA20_004412"
     CDS             1319..1600
                     /locus_tag="QBA20_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294663.1"
                     /translation="MNPMLDYCFTSAPQQTITHTGIKNLLWIIKKNLQQKGDPDLIPC
                     AYGLCDRQVSPDKAYTAELIYMHGTMMRKKKRKYCSEICAEKDQMAHEL"
BASE COUNT          499 a          399 c          411 g          440 t
ORIGIN      
        1 agcattatat agttctactc tggcgagata cgttggcata ttcatttcct tactggttgt
       61 gtgagaactt cagtaaagat accaccaaag cccggaagtg gtgaaataaa accgggcaca
      121 acacgaaggc gcatttccgg tatccataaa gagtcggtct tgtctgtgaa atttaaatgg
      181 tgggagtgcg cttccggttg taaataacga cattgctgtg tgaagtacca gttggcggca
      241 tcggtttaat tgctggctga tgtccgccct ttttaaagtg aattttgtga tgcggtgaat
      301 gcggctatgc gcacgcggca cagttaaaag tcatgttagt ccttattggt ttgggtggga
      361 aagccaactg taattgttaa ctggttaacg tcacctggag gcaccaggca ccgcatcaac
      421 aaagttcact tcggtgatga aaggaaagag aaaatgttga atgtagctat tgaaaaccag
      481 aacgggtgga attatagtgc acctgcacct cataaaacgg gtgccgggat tgctaccccg
      541 acgatgacta ctgcgcataa ccgcgctcag gcggtttttt tatgcgtcaa gcacagccac
      601 attcagatta tggtggggcg tgcagggcag ccgcaaggct ggccggtttc ggtagtcacc
      661 ggttgtagca accctgtgcg tctcaccacc catgagattg ctacctctgg tggtgagtca
      721 tttaaactga ctatcgaggc tgccatcatg gctactatcc ttaccctttc tcaccctgac
      781 gcaaccatcg aaaatggtcg cgctgtcact acgtctgttg cggttgcgga gttcttccgg
      841 aaaatgcaca aaaacgttat tcagaagatc gaaactctcg aatgctcgcc ggagtttaac
      901 cggcttaatt ttaagcccgt cacctatacc gacgccaaag gcgaaaaacg cccaatgtac
      961 caaatcacca aaaacggctt cgttttcctg gtgatgggct tcactggcaa aaaagccgcc
     1021 gcattcaagg aagcctacat tgctgagttc gaccgcatgg aggccgaact gcgccagaat
     1081 aacaccccac ctgctgacaa gatgattccg ggtgatggtc gcactctggt tgttcacttc
     1141 gacaaattcg gcaatgtcga attcaccgaa accgttcctg atggcgcact ggtctgtacc
     1201 ctggagactt tccggtttta tctggaaaaa cagggatgga ctcttgttaa ccggggcgca
     1261 attaaaaata tgactgtgga acaattactt tctttaaaat aaaaatggag ctacggatat
     1321 gaaccctatg cttgattatt gttttacctc tgcacctcag cagaccataa cacacaccgg
     1381 aatcaaaaat ttgttatgga taatcaaaaa aaacctccag cagaaaggtg atcccgattt
     1441 aattccctgt gcttatggcc tgtgtgacag acaagtaagc cctgataagg cttacactgc
     1501 tgaacttatt tacatgcacg gaaccatgat gaggaaaaag aaacgcaaat attgtagtga
     1561 aatctgcgcc gaaaaagacc agatggcaca cgaactttaa ttaactgact attcgaaact
     1621 gaatttatgc cagcaatggc agggattcgc tcaaccttaa ttaaggagaa aaacatgaac
     1681 accaattatg aagccactgt tgcaactact gataacattg ttcacgaggt ttacctggaa
     1741 ggaaagcat
//
LOCUS       DAOFMX010000239         1717 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.241, whole genome shotgun sequence.
ACCESSION   DAOFMX010000239 DAOFMX010000000
VERSION     DAOFMX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.241"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(110..577)
                     /locus_tag="QBA20_004413"
     CDS             complement(110..577)
                     /locus_tag="QBA20_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDQ6294664.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="QBA20_004414"
     CDS             complement(643..882)
                     /locus_tag="QBA20_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HDQ6294665.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="QBA20_004415"
     CDS             complement(1017..1622)
                     /locus_tag="QBA20_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDQ6294666.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          379 c          305 g          484 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatccattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAOFMX010000240         1605 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.242, whole genome shotgun sequence.
ACCESSION   DAOFMX010000240 DAOFMX010000000
VERSION     DAOFMX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.242"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(1..836)
                     /locus_tag="QBA20_004416"
                     /pseudo
     CDS             complement(1..836)
                     /locus_tag="QBA20_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            1340..1549
                     /locus_tag="QBA20_004417"
     CDS             1340..1549
                     /locus_tag="QBA20_004417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294667.1"
                     /translation="MLKLFNEAKTQAEVDRAVSEINSKLSSIQLEYLSLIDELTISKS
                     NEAALKAKLTEIEKFKFQSEDCEPR"
BASE COUNT          424 a          379 c          387 g          415 t
ORIGIN      
        1 gctgctctac ggtcatattt ttaattgcgc tccggtttac aagagcccac ccctgttttt
       61 ccagataaaa ctggaaagta tccagggtac agaccagtgc accgtcagga acagtttcgg
      121 tgaatctgat attgccgtgt tcgtcgaagt gcacaaccag agtgcgaccg ttacccggaa
      181 tcatcttgtc agcggacggg gtattattct ggtgcagttc tgcctccatg cggtcgaaca
      241 tcagcgatgt aggcctcttt gaatgcagcg gcttttttcc cagtgaagcc catcaccagg
      301 aaaacgaagc cgtttttggt gatttggtac attgggcgtt tttcgccttt ggtgtcggtg
      361 taggtgacgt cctcaaaatt gagggcgtta aattcaggtg agcattcagt atttgcgatg
      421 gcacgtaaca cattgtcgtg tcgtttgtgg aagaactctg caaccgcaac agacgtagtg
      481 acagcgcgac cgttttcgat ggttacgtca gggtgagaaa gggcagggat agtagccatg
      541 atggcatcct cgagtgataa gttaattaac tcaccaccga ggttttccac gaccataagg
      601 gtggtgagac gtacaggggt ggaaataccg gtcactcgag aacccggcca gtcttgcgac
      661 tgccctgcac atcccaccat aatttgaatg gagctgtgca ttacgcataa aaaaaccgct
      721 tcagcgcggc tatgcgctcg agtaacgttc gggtttccac gcccggcccc cgttttatga
      781 ggtgcagatg cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc
      841 tcttaccttt catcaccgaa gtgaactttg ttgatgcggt gcctggtgcc tccaggtgac
      901 gttaaccagt taacaattaa cgccggatac agggaaaccc ataaccaccc gatacgtggg
      961 aactaatcgc tttttaactg ttccgcgtgc gctcagccgc attcaccgca tcacaaaatt
     1021 cactttaaaa agggcggaca tcagccagca atgaaactga tgccgccaaa ggtaccaatc
     1081 aacatggagt gttgtggcgt ggttgtcact taagcgtatg gtcaacctga caacccggtg
     1141 tcctcaacgg ggaaggaata actccgccat acttaccgcc gcgtcatttc gcgttgtgtg
     1201 cctgctttta actacgtcag gcgaggtggt tcctgttatt ccccaacaac aagaaatttg
     1261 tataatctga ataccccaac aataagaaga gtgagcaagt gatgtattag cgatgtcagc
     1321 tctcaaagag actgcatcaa tgttaaaatt attcaatgaa gcaaaaactc aggctgaggt
     1381 tgatcgcgct gtgtctgaaa taaactctaa gttgtcaagc attcagcttg aatatttgtc
     1441 tctcattgat gagctgacaa tctctaaaag caatgaggct gcgctcaaag caaaactcac
     1501 ggagatagaa aagtttaagt tccaaagtga agactgcgaa cctcgctaat tgattactgg
     1561 aacgattgtg tatgccagac agtgcaatac tagctctggc aagat
//
LOCUS       DAOFMX010000241         1602 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.243, whole genome shotgun sequence.
ACCESSION   DAOFMX010000241 DAOFMX010000000
VERSION     DAOFMX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.243"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(335..481)
                     /locus_tag="QBA20_004418"
                     /pseudo
     CDS             complement(335..481)
                     /locus_tag="QBA20_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            535..717
                     /locus_tag="QBA20_004419"
     CDS             535..717
                     /locus_tag="QBA20_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HDQ6294668.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(818..1147)
                     /locus_tag="QBA20_004420"
     CDS             complement(818..1147)
                     /locus_tag="QBA20_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDQ6294669.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1319..>1602)
                     /locus_tag="QBA20_004421"
     CDS             complement(1319..>1602)
                     /locus_tag="QBA20_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294670.1"
                     /translation="FVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLND
                     AVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          436 a          368 c          376 g          422 t
ORIGIN      
        1 atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag aataaccagt
       61 tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg gcgacggcgg
      121 aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc tctgtaatgc
      181 ggtatccaac ccgcgaatat cagcatgatt caccgtcgga tttactgcta cgtcgccctc
      241 agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg acacggaaag
      301 tcatatcagc gtcaacggag caccgtattg acgcttattt attggtgaga actacgttcc
      361 atggcaggag ttcgtcaaca cggttggagg gccattcagg cagtatgctc agaatatggc
      421 gcagatacgc ttccggatcg ataccgttca gacggcaggt gcctgctttt ccggcaacaa
      481 gcaggaatct cagcgctgaa gcaggaatta gaggtaaaaa cgccctaccg ggccatgaat
      541 cacccggtca ttggagtagt aaccaaagca gatttagcca gcatggaaca aatatctttg
      601 gtgaaaagtt ggttacggga agctggagcg cacaacgtgt tagtaaccag tgcagttaat
      661 aacaatggag ttacagaact ttttgccttg ctgcatacag aagaaggctg ttgttaattt
      721 cgattggttc tcagatgaga atgatgacgg tgatacacag ataagaatca atgagcaatg
      781 agggttgccg ggcaaccctc attgaataaa acgggaatta cttcgcttcg ccgtttttca
      841 tttcgcccat agctttcagc tttttggaga tatcgcggcg ttctttggag agctcggcat
      901 ttttgatgat gtaatcgtca acgcgatctt catagtcacc tttcatgctg gcgatgatgc
      961 cctggattgc ttcaacgctc atccctggct tgatgtaatc gctcaggttg tccagcaaca
     1021 ggacgcgctt ctggttgtca cggatctttt tctcaacgtc ctgaatttca cgttgcagtt
     1081 tgttcttacg acggaacaga cgaacaaatt ccagtacgtc ctggaatgaa ggcttggtag
     1141 tttccatttt tatacccctg ataatgtgag agtcggattc gtttaagcaa ccgctattcg
     1201 ttagggccaa cattactcat agctgccgca gatgacaatg cttttatccc ttttcactat
     1261 catacccttt atccttgctg aatcgaagca gcagcaagat gattctgaag ttcaggaatt
     1321 atttgcgcaa ggcccggcaa atcagattcg atagcaactc aagctgatgt gccgtttcca
     1381 tgttcagcca cacataacca tagattggtg tttccacgac tttcaggtca tggtggttat
     1441 cgagcaactg acgcagctct tccacagcat cgttcaattt ttccgtattg gcaaaaaccg
     1501 gctgcggatt accttcgtac agcgcatgaa caaggctcag cagtatttgc tgcatcatgt
     1561 gctgggtatc acaaagtttt tgcgcgttca ataacacgaa at
//
LOCUS       DAOFMX010000242         1597 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.244, whole genome shotgun sequence.
ACCESSION   DAOFMX010000242 DAOFMX010000000
VERSION     DAOFMX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.244"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..636)
                     /locus_tag="QBA20_004422"
     CDS             complement(<1..636)
                     /locus_tag="QBA20_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDQ6294671.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="QBA20_004423"
     CDS             complement(750..1322)
                     /locus_tag="QBA20_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HDQ6294672.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          476 a          337 c          317 g          467 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacaactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtatttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAOFMX010000243         1562 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.245, whole genome shotgun sequence.
ACCESSION   DAOFMX010000243 DAOFMX010000000
VERSION     DAOFMX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.245"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="QBA20_004424"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="QBA20_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDQ6294673.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="QBA20_004425"
     CDS             578..1027
                     /locus_tag="QBA20_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDQ6294674.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /locus_tag="QBA20_004426"
     CDS             complement(1028..1261)
                     /locus_tag="QBA20_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDQ6294675.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="QBA20_004427"
     CDS             complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="QBA20_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDQ6294676.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
BASE COUNT          355 a          366 c          422 g          419 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagagacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tg
//
LOCUS       DAOFMX010000244         1553 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.246, whole genome shotgun sequence.
ACCESSION   DAOFMX010000244 DAOFMX010000000
VERSION     DAOFMX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.246"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..776
                     /locus_tag="QBA20_004428"
     CDS             join(<1..274,274..776)
                     /locus_tag="QBA20_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HDQ6294677.1"
                     /translation="TPRAGRPWAEHRKIINGMFWVLCSSAPWRDLPERYGAWKTVYNR
                     FNRWSKSGVINIIFNRLLSLLDANGFIDWSATALDGSNIRALKCAAGGSKKHPDIDGD
                     NGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAHESQFAQRLLDGIGVQRQNGSMKR
                     RGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVER
                     CFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            891..>1553
                     /locus_tag="QBA20_004429"
     CDS             891..>1553
                     /locus_tag="QBA20_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HDQ6294678.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHS"
BASE COUNT          408 a          391 c          422 g          332 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt attcagggcg cgcattgcgc aacgagctga
      541 aaaataacgg tataaaggca gtaatccccc gaaagtcaaa tgagaaaatg gcatcggatg
      601 gacgtgcaca gcttgatcgt gatgcgtact gcaatcgtaa tgtcgttgaa cggtgctttg
      661 ggcggctgaa agaatatcgc cgcatcgcca cgcgttacga caaaacggcg agaaattacc
      721 tggcgatggt gaaactgggc tgcatccgac tcttttatca acgcttacgt aattaaggga
      781 cacagcctaa atagctgcgc ggaatagtag atccctttga gggaacttag cccggattgt
      841 gcgatctgat caatcgccaa atcaaaacaa atcaccaacc ggactgagca atgccgatca
      901 tagcaccaat ttcccgtgac gaacgacgcc tgatgcagaa agccatccat aaaacacacg
      961 ataaaaatta tgcccgcaga ctgactgcca tgctgatgct gcaccggggc gaccgtgtca
     1021 gcgacgttgc cagaacgctc tgctgcgccc gttcctctgt tggacgctgg attaactggt
     1081 tcacgcagtc gggtgttgag ggactgaaat cattacctgc cgggcgtgcc cgtcgctggc
     1141 cgtttgagca tatctgcaca ctgttacgtg agctggtaaa acattctccc ggcgactttg
     1201 gctaccagcg ttcacgctgg agtacagaac tgctggcaat aaaaatcaat gagataaccg
     1261 gttgccagtt aaatgccgga accgttcgcc gctggttgcc gtctgcgggg attgtgtggc
     1321 gaagggctgc gccaactctg cgtatccgtg acccgcataa agatgaaaag atggcagcaa
     1381 tccataaagc actggacgaa tgcagcgcag agcatccggt cttttatgaa gatgaagtgg
     1441 atatccatct tcatcccaaa atcggtgcgg actggcaact gcgcggacag caaaaacggg
     1501 tggtcacgcc gggacagaat gaaaaatatt atctggccgg agcgctgcac agc
//
LOCUS       DAOFMX010000245         1549 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.247, whole genome shotgun sequence.
ACCESSION   DAOFMX010000245 DAOFMX010000000
VERSION     DAOFMX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.247"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            351..476
                     /locus_tag="QBA20_004430"
     CDS             351..476
                     /locus_tag="QBA20_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDQ6294679.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="QBA20_004431"
     CDS             complement(921..1397)
                     /locus_tag="QBA20_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6294680.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          357 a          405 c          385 g          402 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggt gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcctc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atcctggatg
     1501 cgacaacgtt gtgtcaccct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAOFMX010000246         1492 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.248, whole genome shotgun sequence.
ACCESSION   DAOFMX010000246 DAOFMX010000000
VERSION     DAOFMX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.248"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..904)
                     /locus_tag="QBA20_004432"
     CDS             complement(<1..904)
                     /locus_tag="QBA20_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294681.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYY"
     gene            complement(901..1429)
                     /locus_tag="QBA20_004433"
                     /pseudo
     CDS             complement(901..1429)
                     /locus_tag="QBA20_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT          481 a          359 c          290 g          362 t
ORIGIN      
        1 aatagtaaat taagcacggt acatagcaga gtaaaaaata ggcttcaaac caaatccgcc
       61 attccacaag cgttgccaat accgacacca gcgtaatcca catcatttca ataacccgca
      121 atatgaataa ccgaagccaa agcggcttgg caaaaaaggt cgacaacgta ttttcactgg
      181 agaaaaacag atcgatttcc tgcaaaaaaa acctgcccag aaaagcgggc cgcttggaga
      241 agcgcagaaa aaacagatta atcgccaaca ctacgccaca aagaatgagc aatgcaatta
      301 tcgatttaaa cgttaaccac tgcccctctg tcacaaaaga atcactgtta cgcaacgggc
      361 tgatagtaag caggttaaca accgttgaga tgcacaccga aagcagcaca tagcgagtca
      421 gacttttata ttttactttt ttcgctactg aaatagcgac ctgcttgaca taactggtgc
      481 ggatcatccg aaacagcaga aagatgacca acggcccaag taacgaaatc aggaacagcg
      541 catataacga gggtagaagc tggtatagcc cccaactcat tgcactcaac atgattaacg
      601 acagaaatcc tgccaccaga ggttgtgtaa gaaataattc tattttgtgg ttctgctttt
      661 tcttaaacca cgaaaaatcg atattcttcg tgacgcattt ataagcccag tacgccttga
      721 gatcaaaaat aaaattatac agcaccaggg ccagaatccc actaactaca ccaatatcct
      781 ctgatggaaa agtaaaccct gcatttttcc aataaaagcc tatcagaata agtccaatag
      841 cataaatgta tatcagattt cgtttatcct gaaagcgaat aaaagttaaa taatcaggaa
      901 tcatttgaat acctttgcat cactaaatgc tttacgacaa tgcataactc gttgctgatc
      961 cacgccagca aaatcattta acatattccc caaatagggc ttattactta tttcaaggaa
     1021 agctaccggg gttggcatat tcagaatatg tatcgagaga ttcagtccat ttcaccgtat
     1081 gagttaagtg taatgagaga ttttctttga taattgacgg agcgacctcg ctttttgctg
     1141 ttacgttcat cagaacctga tgctctggcg aagcgatatc cagtccggca agataatcgc
     1201 gcattgcctg aacgccgtct tccatcaaac gcgtgtgcca ggcaccgctc acaccgagtt
     1261 taaccggttc gtatccagcg gccatcagca gcgtggtaaa ttcattcaac gcagcctgcg
     1321 ttcccccaat aacctgctgg cgcggcgtgt tatcacagct aatatccagc gcaatgcctg
     1381 attccgtaat catcgtctgc agttgttcgc ggttgatgcc tttaaccgcc tgatcttacc
     1441 cagcaatagt ggacacgcgg ctaagtgagt aaactctcag tcagaggtga ct
//
LOCUS       DAOFMX010000247         1470 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.249, whole genome shotgun sequence.
ACCESSION   DAOFMX010000247 DAOFMX010000000
VERSION     DAOFMX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.249"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(5..184)
                     /locus_tag="QBA20_004434"
                     /pseudo
     CDS             complement(5..184)
                     /locus_tag="QBA20_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            708..992
                     /locus_tag="QBA20_004435"
     CDS             708..992
                     /locus_tag="QBA20_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDQ6294682.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHPLPVPR"
     gene            992..1267
                     /locus_tag="QBA20_004436"
     CDS             992..1267
                     /locus_tag="QBA20_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDQ6294683.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          401 a          369 c          362 g          338 t
ORIGIN      
        1 aaaattactc tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg
       61 caaaaacagc agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa
      121 caagtcccat tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca
      181 gccctaacag aataagttta cgcgtaaatc gcccggaatc cagaagagca ttaagtatca
      241 cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta
      301 gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact
      361 ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg
      421 cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc
      481 gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac
      541 tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc
      601 tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg
      661 tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggtatg caaatgaaaa
      721 acaataccgc acaagcaaca aaagtaatta ccgcgcatgt gccattacct atggctgata
      781 aagtcgacca gatggccgcc agactggaac gctcccgggg ctgggttatc aaacaggcgc
      841 tttctgcatg gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa gccctggacg
      901 atgtgacatc cggacaggtt atcgaccatc aggctgtaca gtcctgggcg gacagcctca
      961 gtactgacca tccgttaccg gtgccacgct gatggaactg aagtggacca gtaaggcact
     1021 ttctgatttg gcgcggttat atgattttct ggtgctggcc agtaaacctg ctgccgccag
     1081 aacggtgcag tccctgacac aggcgccggt cattctgtta actcatccac gtatgggaga
     1141 acagttgttt cagtttgaac ccagggaggt cagacggatt tttactggcg agtacgaaat
     1201 ccgttacgaa cttactggcc agactattta tgtattgcgc ctgtggcaca cacgagagaa
     1261 caggtagatt gggtgtggag gcaactttaa catagcctgc aggtattaat tctggagcat
     1321 tgttatcagg ccagatattt tgcattccat taaataatct tgtaggagat aaaataaatt
     1381 acgatgaaat aacgaaaatt accgctcgtg actggcggca atatcgcgct ccgggctggc
     1441 aaataaccca tcagaaacgt tattgccaga
//
LOCUS       DAOFMX010000248         1402 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.250, whole genome shotgun sequence.
ACCESSION   DAOFMX010000248 DAOFMX010000000
VERSION     DAOFMX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.250"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..390
                     /locus_tag="QBA20_004437"
     CDS             <1..390
                     /locus_tag="QBA20_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDQ6294684.1"
                     /translation="WTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERN
                     IKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDC
                     RIRSNNCYGQVIRRDQESALACWGIDQ"
     gene            387..854
                     /locus_tag="QBA20_004438"
                     /pseudo
     CDS             387..854
                     /locus_tag="QBA20_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            842..991
                     /locus_tag="QBA20_004439"
                     /pseudo
     CDS             842..991
                     /locus_tag="QBA20_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            1073..1360
                     /locus_tag="QBA20_004440"
     CDS             1073..1360
                     /locus_tag="QBA20_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294685.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT          368 a          312 c          379 g          343 t
ORIGIN      
        1 tggaccatct gtcgtggtgc cacaatggtg gatggtaagc ccgtcatacc gggaatgaag
       61 ctgtcgaagg aaaaatgcga ccaggttaac gctattgaac gtgataaggc gctggcatgg
      121 gtggagcgca atattaaagt accactgacc gaaccacaga aagcgggtat agcgtcattt
      181 tgtccctata acattggccc aggtaagtgt ttcccgtcga cgttttataa gcggctgaat
      241 gccggtgatc gtaagggcgc atgcgaggcg attcgctggt ggataaaaga tggtgggcgc
      301 gattgccgca tacgttcaaa taactgctat ggacaggtta ttcgtcgtga ccaggaaagc
      361 gcattagcct gttgggggat agatcagtga gctgagtcgc cgcgattatt tatgctctgg
      421 ttatctgcat catcgtctgc ctgtcgtggg cggtcaatca ttaccgtgat aacgccatcg
      481 cctacaaaga acagcgtgat aaaaaagtca gtgagctgaa gctggcgacc gccaccatta
      541 ctgacatgca gcaacgccag cgtgctgctg atgtactcga tgctaaatac acgaaggagt
      601 tggctgatgc gaaagctgaa aatgatgctc ttcggcgcaa gcttgataat ggtggccggg
      661 tgctcgtcaa aggaaaatgc tctgtgccat cctcagccga aacctccagc gcctccggca
      721 tgggcaatga tgccaccgtc gaactctctc cagttgctgg acgaaacgtt ctcggtatcc
      781 gggacggaat catcagagac cagacagcac tgagaacgct tcaggaatat atcaggacgc
      841 aatgccttcg atgatagaga taattttact catcatcctt cacatctggc tctgtagaca
      901 gggtggtgat cacttctgga gtgaatccag attaaacatc tcattgctga tgcttaaagt
      961 tgagcatttg gcgcgcggta aggggctgcg tttagataag agccagtcat cacaaacacc
     1021 aggatttagc ctcgcattcg cggggctttt tattgcaaca aaggtaaaga cgatggatga
     1081 agaatatcgt aaagacctgc aactgtggtt tggtcttacg catgcgtcgt tctgcgtgat
     1141 gccgcgagta ttcatggagg ctatgcctcc agaatggcaa gagaagatgg ctcagttgct
     1201 ttttgagtat agcgacacga tcaaaacgga tgtctgcgga gttcacagct gttttgttac
     1261 tgccaaagac ggcaacaacc gctttatgag gatgccagaa gatattctga actatcgtca
     1321 tcctcgacgt gaattcatcg aatcatttct gaagaagtag ccattacaaa gcctatctac
     1381 gggtgggttt gataatggct ta
//
LOCUS       DAOFMX010000249         1383 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.251, whole genome shotgun sequence.
ACCESSION   DAOFMX010000249 DAOFMX010000000
VERSION     DAOFMX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.251"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..253
                     /locus_tag="QBA20_004441"
                     /pseudo
     CDS             1..253
                     /locus_tag="QBA20_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1383
                     /locus_tag="QBA20_004442"
     CDS             746..>1383
                     /locus_tag="QBA20_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDQ6294686.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
BASE COUNT          340 a          320 c          422 g          301 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gctaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 act
//
LOCUS       DAOFMX010000250         1346 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.252, whole genome shotgun sequence.
ACCESSION   DAOFMX010000250 DAOFMX010000000
VERSION     DAOFMX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.252"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(3..485)
                     /locus_tag="QBA20_004443"
     CDS             complement(3..485)
                     /locus_tag="QBA20_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDQ6294687.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="QBA20_004444"
     CDS             complement(476..778)
                     /locus_tag="QBA20_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDQ6294688.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="QBA20_004445"
     CDS             1050..1322
                     /locus_tag="QBA20_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294689.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          397 a          337 c          287 g          325 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagcaaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccgaccgct gaaacg
//
LOCUS       DAOFMX010000251         1277 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.253, whole genome shotgun sequence.
ACCESSION   DAOFMX010000251 DAOFMX010000000
VERSION     DAOFMX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.253"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="QBA20_004446"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="QBA20_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HDQ6294690.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          360 a          229 c          284 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttatt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag aactttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAOFMX010000252         1276 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.254, whole genome shotgun sequence.
ACCESSION   DAOFMX010000252 DAOFMX010000000
VERSION     DAOFMX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.254"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..350
                     /locus_tag="QBA20_004447"
     CDS             <1..350
                     /locus_tag="QBA20_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294691.1"
                     /translation="PSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFD
                     ARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDG
                     VYYRPLQKLINGT"
     gene            374..918
                     /locus_tag="QBA20_004448"
                     /pseudo
     CDS             374..918
                     /locus_tag="QBA20_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            942..>1276
                     /locus_tag="QBA20_004449"
     CDS             942..>1276
                     /locus_tag="QBA20_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDQ6294692.1"
                     /translation="MSVVISGALTDGAGISMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWHNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVA"
BASE COUNT          360 a          275 c          333 g          308 t
ORIGIN      
        1 atccatccag gcctgttgtt gcagagcttg gcgatgattc aggctggcat tttttcagcc
       61 agagaaaccc ggataacagc atagtgttca ctgttaacgg acaggtaatt ccgttaaatt
      121 acggaaactt cgatgcccgc tataaatatc gaacagaggg ggtacaggat gtacggtatg
      181 gccatgaaat gtattacagc cccggcagta acaccgtttc gtggagattt tgcgcacctt
      241 cgggacacgg gctgtcaggg atggcgatat cggataccgg ccgtaactca gcggataacg
      301 ttgacggtgt gtattaccga ccactgcaaa aactgattaa tggcacctag tataacgtag
      361 cgagtattta acaatgttgc atttaaaaaa tattactacg ggtaatccga aaacagcaga
      421 acaatatcag atgacaaaac gatattcggt cacctggctt ttttcagaag acggaaaaaa
      481 ctggtatgaa gagctgaaga atttcgccag gacacaatta aaataactta caccggagac
      541 ggtcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
      601 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
      661 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
      721 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgttgct ggggctaatc
      781 agcgacgaag accgggaaaa actgaaagcg taccgcattt acgcgaaatc gctgcaggcg
      841 atggatttca gtgtcatcac tgataaaacc tcatacaacg ccattgaatg gcccgcttct
      901 ccggaaattt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
      961 gtgcgctgac tgatggcgca ggtatctcca tgtccggata ccatattatt ctgaaatccc
     1021 gggtaaacac cccggaagtg gtgatgcaca ctgttgctga tgtgatgaca ggaaacgatg
     1081 gtgaatactg tttccatgcg cggactggaa aatatggtgt gtatctgaaa caggactggc
     1141 acaacgagta caacgttggc gacattgctg tatatgagga ctcaaagccc ggtacgctga
     1201 atgactttct gattgctcct gatgagggcg acctgaaacc agatgtcgtc aaacgctttg
     1261 aggaaatggt ggcgca
//
LOCUS       DAOFMX010000253         1275 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.255, whole genome shotgun sequence.
ACCESSION   DAOFMX010000253 DAOFMX010000000
VERSION     DAOFMX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.255"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            34..435
                     /locus_tag="QBA20_004450"
     CDS             34..435
                     /locus_tag="QBA20_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDQ6294693.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="QBA20_004451"
     CDS             447..818
                     /locus_tag="QBA20_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6294694.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="QBA20_004452"
     CDS             805..>1275
                     /locus_tag="QBA20_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6294695.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          285 c          396 g          291 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAOFMX010000254         1264 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.256, whole genome shotgun sequence.
ACCESSION   DAOFMX010000254 DAOFMX010000000
VERSION     DAOFMX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.256"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(41..100)
                     /locus_tag="QBA20_004453"
     CDS             complement(41..100)
                     /locus_tag="QBA20_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDQ6294696.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1247)
                     /locus_tag="QBA20_004454"
     CDS             complement(339..1247)
                     /locus_tag="QBA20_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294697.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          249 a          341 c          308 g          366 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttacgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgtt
//
LOCUS       DAOFMX010000255         1171 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.257, whole genome shotgun sequence.
ACCESSION   DAOFMX010000255 DAOFMX010000000
VERSION     DAOFMX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.257"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            502..924
                     /locus_tag="QBA20_004455"
     CDS             502..924
                     /locus_tag="QBA20_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HDQ6294698.1"
                     /translation="MRQTEDKQHQNIEPEEGTPCNQTGEDNCPDCGAVMGDATYQETF
                     DEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGEDELNWKKQVLIAAVYGL
                     CANPACIAYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            902..>1171
                     /locus_tag="QBA20_004456"
     CDS             902..>1171
                     /locus_tag="QBA20_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HDQ6294699.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHH"
BASE COUNT          375 a          273 c          299 g          224 t
ORIGIN      
        1 aaagaagcac cgattggtat cgaggtcatc cccgcgcacg tcactgaata tctgaacaag
       61 gtactgactg aaaccgatca tgccaaccct gatccggaaa tcgtggatat tgcctgcggt
      121 cgctcctctg ccccgatgcc gcagcgtgta acagaagaag gaaaacagga tgatgaagaa
      181 aaaccgcagc catctggcgc aatggcagat gaacaggcaa cggctgaaac aatggaaccg
      241 gatgcaactg aacatcatca gaacacgcag ccgctggatg ctcagtcaca ggtaaattct
      301 gttgatgaga aatatcagga actgcgggaa gaactccatg aagcccggaa aaacattccg
      361 cccaaaaatc ctgtcgatgc agacaaatta ctggctgcct ctcgcggaga atttgttgaa
      421 gggattagcg acccgaatga tccgaaatgg attcccggtc atcatatctc ctcaaatgaa
      481 gtcaaaaaaa cggaaaatga agtgcgacag actgaagata aacagcacca gaacatcgaa
      541 ccggaggagg gaacaccctg caatcagact ggcgaggata actgccctga ctgtggcgcg
      601 gtgatgggag acgcaactta tcaggaaact tttgacgaaa aaaaaccaga tgaagcacag
      661 gaagaagagc cgaagaaaac ggaaaaagct gatgatttgt taccggagaa cgctggcagc
      721 gatcagcata atgatagcaa taatgaaact ggcgaagatg aactcaactg gaaaaaacag
      781 gtgttgattg cggcggttta cggtttatgt gcgaatcctg cgtgcatagc ctatgcgcct
      841 gcaatccctg acatcgcaat gatgattgcc aacaagcttg aaaattttgg agtgaccagt
      901 gatgagcgct tacccgattt ttgatcgtat tgaagaaatg gcatgggctc gccactacca
      961 gcagatcgtt cgtgaagaaa aagaaacgga actggcggac gacctggaaa aaggtctgcc
     1021 tcagcacctg tttgaatcgc tctgcatcga ccatttgcaa cgccacggtg ccaataaaca
     1081 ggcaatcagc cacgcatttg atgatgatgt tgagtttcag gagcgcatgg cagaacacat
     1141 ccggtacatg gctgaaacca ttgcccacca c
//
LOCUS       DAOFMX010000256         1159 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.258, whole genome shotgun sequence.
ACCESSION   DAOFMX010000256 DAOFMX010000000
VERSION     DAOFMX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.258"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            115..>1159
                     /gene="cadB"
                     /locus_tag="QBA20_004457"
     CDS             115..>1159
                     /gene="cadB"
                     /locus_tag="QBA20_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDQ6294700.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctg
//
LOCUS       DAOFMX010000257         1158 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.259, whole genome shotgun sequence.
ACCESSION   DAOFMX010000257 DAOFMX010000000
VERSION     DAOFMX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.259"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..388
                     /locus_tag="QBA20_004458"
     CDS             <1..388
                     /locus_tag="QBA20_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294701.1"
                     /translation="LVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(389..1033)
                     /locus_tag="QBA20_004459"
     CDS             complement(389..1033)
                     /locus_tag="QBA20_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6294702.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          295 a          264 c          278 g          321 t
ORIGIN      
        1 cctggtgctg gtggcaattc tgattatgct gccgactctc ggcatcggtg atatgaacaa
       61 tctctataaa tggctgttga atcttaactc ggtagtgatg ccgctgcgtt atttatgggt
      121 atttgttgca tttattgcag tcgttcgcct ggcgcagaaa tataaaccag aatatgtctt
      181 tattcgtaat aagcctctgg caatgaccgt cgggatttgg tgttttgcct ttaccgcctg
      241 tgcctgtttg acggggatct tcccgaaaat ggaagccttc actgcagagt ggaccttcca
      301 gttggcgctg aatgttgcaa cgccgtttgt gctggtagga ttaggactga tattcccgct
      361 gctggcgcgt aaagcgaata gcaaataatt attgtggtcg gtgacgctga ctctggcaga
      421 tctgacccgt ttttacataa tcgagaaatg cgcgcaagcc gcaggacata tactggcggt
      481 ttgggtaata gacttggaag cctggacgct ctgtgatcca gtcttccagt acacattcta
      541 aacgaccagt atcaaggtac tccttgatct gttcatagag cagatacccg ataccagcac
      601 ccatcaaaac ggcctccagt tcagcatcaa catcatccag aatgatattt cctgcgacag
      661 caatttccag ctcctttgca aattgccagt ggaaaggttt gccgctggga taacgaaaaa
      721 caacacattg atgattaagc agatcatgag gatggcgggg tttgccatat cttgcaaagt
      781 actctggcgt tgctgctaca cataatttca ctggtgggcc gatcgccacg ctgatcatat
      841 ctttttcgac gatgccacta agtcgaacgc cagcgtcaaa gccctgttgg acgatatcga
      901 taagagaatc gtcagttgtg agctctactt taatatcagg atattcacgt gtgaaaccaa
      961 ccagtaaagg cattaaaaat atacgtgcag ctacccgggc ggcattgatt ttgagcgttc
     1021 ctgtcggtgt cagacgaaaa tcattcatct catctaacat gatctgaatt tcgtcgaaag
     1081 ctgggcgcaa tcgttcatag agattagaac ccgcttccgt taacgatacg cttcgggttg
     1141 tccgattgaa taagcgaa
//
LOCUS       DAOFMX010000258         1140 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.260, whole genome shotgun sequence.
ACCESSION   DAOFMX010000258 DAOFMX010000000
VERSION     DAOFMX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.260"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..730
                     /locus_tag="QBA20_004460"
     CDS             <1..730
                     /locus_tag="QBA20_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDQ6294703.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="QBA20_004461"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="QBA20_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DAOFMX010000259         1128 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.261, whole genome shotgun sequence.
ACCESSION   DAOFMX010000259 DAOFMX010000000
VERSION     DAOFMX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.261"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(57..326)
                     /locus_tag="QBA20_004462"
     CDS             complement(57..326)
                     /locus_tag="QBA20_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ6294704.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            383..1051
                     /locus_tag="QBA20_004463"
     CDS             383..1051
                     /locus_tag="QBA20_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDQ6294705.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          311 a          257 c          230 g          330 t
ORIGIN      
        1 accagccaac cgcagcacgt cctgcatacg tcgtgtctgc ggtttttctt tttcgcttac
       61 attgtgtctg gttcttccgg ccactcaata tcaggtgcag ttgatgtatc aacacggttc
      121 agcaacaccc gatacttctt ccaggcttcc agcaacgagt tttcttcctc cgttgcgatc
      181 tccagatcta cagcatcctg aagtggcgca atatgctcac tgaatccctg gatgtagaac
      241 tgtgtggtga cggtcttcca gccattcggc tcctgctgta tcgaagcata ccaggctatt
      301 tcaatatcgc tatgctgcgg cagcatttaa ccccttgtaa ttcatcgcca taattgattt
      361 aattcacaaa taaaactata atatggtgaa attaatgaaa aaaaacacag atgatggggc
      421 taaaatttac acaccactta ccctaaagct ttatgactgg tgggttttgg gagtatcaaa
      481 tcggcttgca tggggatgtc ctacaaagga acaccttctt ccacactttc tggaacattt
      541 aggtaacaac catctggata ttggtgttgg aactgggttt taccttactc acgtacctga
      601 gagtagtctg atatctttaa tggatttgaa cgaagctagc ctgaacgcgg catctacaag
      661 ggctggggaa tcaaaaatta aacataaaat tagccatgat gtttttgaac cttatcctgc
      721 ggcgttacat ggtcaatttg attccatttc catgttttac cttcttcact gcctgcctgg
      781 aaatatatct acaaaaagct gtgtaatacg caatgcggcg caggccttaa ctgatgatgg
      841 aactctatac ggagccacaa ttcttggcga tggagttgtg cacaatagct tcggtcaaaa
      901 actgatgcgc atttacaatc agaaaggcat cttttcaaac acaaaagatt ccgaagaagg
      961 cttaacacat atactctcag agcatttcga gaatgttaaa accaaggttc aaggtactgt
     1021 agtaatgttt tccgcttcag ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag
     1081 acgtgctacg gtttccttta cgatttgggg ttgcgacttt acctctat
//
LOCUS       DAOFMX010000260         1122 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.262, whole genome shotgun sequence.
ACCESSION   DAOFMX010000260 DAOFMX010000000
VERSION     DAOFMX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.262"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            13..486
                     /locus_tag="QBA20_004464"
     CDS             13..486
                     /locus_tag="QBA20_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDQ6294706.1"
                     /translation="MAIAFKNIQAGTARKFTLSSANNSTKNAAFYLWGNPSRPVVAEL
                     GDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSP
                     GSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            489..1034
                     /locus_tag="QBA20_004465"
     CDS             489..1034
                     /locus_tag="QBA20_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDQ6294707.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            1058..>1122
                     /locus_tag="QBA20_004466"
     CDS             1058..>1122
                     /locus_tag="QBA20_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDQ6294708.1"
                     /translation="MSVVISGALTDGAGIPMSGYH"
BASE COUNT          320 a          242 c          288 g          272 t
ORIGIN      
        1 tttcagaatg gagtggctat tgcttttaag aatattcagg ccggaactgc cagaaaattc
       61 acgttatcca gcgccaacaa ctccacgaaa aatgcagcgt tttatttgtg gggtaatcca
      121 tccaggcctg ttgttgcaga gcttggtgat gattcaggct ggcatttttt cagccagaga
      181 aacccggata acagcatagt gttcactgtt aacggacagg taattccgtt aaattacgga
      241 aacttcgatg cccgctataa atatcgaaca gagggggtac aggatgtacg gtatggccat
      301 gaaatgtatt acagccccgg cagtaacacc gtttcgtgga gattttgcgc accttcggga
      361 cacgggctgt cagggatggc gatatcggat accggccgta actcagcgga taacgttgac
      421 ggtgtgtatt accgaccact gcaaaaactg attaatggca cctggtataa cgtagcgagt
      481 atttaacaat gttgcattta aaaaatatta ctgcgggtaa tccgaaaaca gcagaacaat
      541 atcagatgac aaaacgatat tcggtcacct ggcttttttc agaagacgga aaaaactggt
      601 atgaagagct gaagaatttc gccagcgaca caataaaaat agcttacacc ggagatggtc
      661 gcgtggtgtg ggtcggtaag gatgtgacag gcatcgagcc acgcaatgcc agcgttatcg
      721 aagttccgga tattaccgct aaccgacgga ttaccgcgcc gggttactgg ttttaccgca
      781 atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat gcccttctgg
      841 ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg ttaatcagcg
      901 acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg caggcgatgg
      961 atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc gcctctccgg
     1021 aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga tatcaggtgc
     1081 gctgactgat ggcgcaggta tccccatgtc cggataccat at
//
LOCUS       DAOFMX010000261         1091 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.263, whole genome shotgun sequence.
ACCESSION   DAOFMX010000261 DAOFMX010000000
VERSION     DAOFMX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.263"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..1001
                     /locus_tag="QBA20_004467"
                     /pseudo
     CDS             1..1001
                     /locus_tag="QBA20_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          307 a          266 c          257 g          261 t
ORIGIN      
        1 actatgttca gtccctgcgc gagttgtaca ccgatgtcat cgaagagttt tactgggttg
       61 cgctcccgct tacgacgcaa aattcccttt cacaatacca acccgaatgg cagtgctggg
      121 aacctgatgt cgaatgggtg cgtcagcccc cgcaagatgc gataaccgct cctgactttt
      181 tctgctttta ccagccaggc atgaccttcg aacaatttgt acgtgagttt gccgaatggt
      241 tttcacaaaa acgtccggcg gcgatgatga tcggcatccg tgcggatgag tcctacaacc
      301 gttttgtcgc catcgccagt ttaaataaac aacgttttgc cgacgataaa ccatggacta
      361 cggccgcacc aggcggtcat agctggtaca tttaccccat ttacgactgg aaagtggctg
      421 atatctggac ctgatatgca aatcatcaaa atctctgcaa cccactgtat aacctaatgt
      481 atcaggcagg cgttcctctg cgtcatatgc gaatttgcga accttttggc ccggagcaac
      541 gacaaggatt atggctctgt cacgttatcg aaccggatcg ctgggctgct atgtgcgcac
      601 gagtcagcgg ggtaaaaagt ggcggcattt acgccggaca tgacaatcat ttctatgggc
      661 atcggaaaat cctcaaacca gaacatttag actggcaaga atatgcatta ttgctgctca
      721 atagcatgcc ggaaaaaaca gctgagcatt accgcaataa aatcgccatt tatttgcact
      781 ggtatcagaa aaaaggcatc gaggttccac aaacccagca aggggacatt ggcgcgaaag
      841 atatcccctc ctggcggcgg atatgcaaag ttttactcaa taacgattac tggtgtcggg
      901 cattatcatt tagtcctacg aaagcgaaga actatcagcg ttataacgaa cggataaaag
      961 gaaaacgtca ggaatggggg atactatgca acaacgatta acgcaggatt taacgcaatt
     1021 tctcgccagc ttgccggaag atgatcgcat caaagcgatt aatgaaatcc gcatggcgat
     1081 ccatcaggtt a
//
LOCUS       DAOFMX010000262         1069 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.264, whole genome shotgun sequence.
ACCESSION   DAOFMX010000262 DAOFMX010000000
VERSION     DAOFMX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.264"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            18..560
                     /locus_tag="QBA20_004468"
     CDS             18..560
                     /locus_tag="QBA20_004468"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294709.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            787..>1069
                     /locus_tag="QBA20_004469"
     CDS             787..>1069
                     /locus_tag="QBA20_004469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HDQ6294710.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          201 c          258 g          296 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgtgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggtg
//
LOCUS       DAOFMX010000263         1066 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.265, whole genome shotgun sequence.
ACCESSION   DAOFMX010000263 DAOFMX010000000
VERSION     DAOFMX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.265"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(570..749)
                     /locus_tag="QBA20_004470"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="QBA20_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="QBA20_004471"
     CDS             843..>1066
                     /locus_tag="QBA20_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6294711.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAOFMX010000264         1020 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.266, whole genome shotgun sequence.
ACCESSION   DAOFMX010000264 DAOFMX010000000
VERSION     DAOFMX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.266"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(50..190)
                     /locus_tag="QBA20_004472"
     CDS             complement(50..190)
                     /locus_tag="QBA20_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294712.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="QBA20_004473"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="QBA20_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDQ6294713.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          206 c          237 g          281 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt ccccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtta
//
LOCUS       DAOFMX010000265         1003 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.267, whole genome shotgun sequence.
ACCESSION   DAOFMX010000265 DAOFMX010000000
VERSION     DAOFMX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.267"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..732
                     /locus_tag="QBA20_004474"
     CDS             <1..732
                     /locus_tag="QBA20_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDQ6294714.1"
                     /translation="PFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVY
                     TGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDAL
                     KSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESW
                     TSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDN
                     NHEQIELLNRRLSESVPCMPHKKAG"
     gene            753..>1003
                     /locus_tag="QBA20_004475"
     CDS             753..>1003
                     /locus_tag="QBA20_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDQ6294715.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
BASE COUNT          322 a          225 c          229 g          227 t
ORIGIN      
        1 ccatttaacg gtctgacacc attaaaaaag tccaagatag aaccagatcc cctcacccgt
       61 gacgaattta ttcgttttat tgaggcttgc cgtcatcaac aaacaaaaaa cctgtggatt
      121 atcgctgtat acacgggtat tcgtcacggg gagctggtat cactggcctg ggaagatata
      181 gaccttaaag cgaggactat aaccatccgc cggaattata caaaactagg cgaattcact
      241 ccaccaaaaa ctgatgctgg taccggaaga acgattcatc tagttcaacc agctattgat
      301 gctcttaaaa gccaagcgga aatgaccatg cttggaaaac agcattctgt agaggtaaag
      361 cagagggaat atgggagaag tactgtgcat aaatgcactt ttgtttttag tcctcaggta
      421 ataaaacagc ggcagttttc cggaccgcac tataaggttg actccatcag ggagtcatgg
      481 acaagtatct taaaacgcgc aggtctgaga cacagaaaat cgtatcaatc caggcatacc
      541 tatgcatgct ggtcacttgc cgcaggagct aatcctagtt ttatcgcaag ccagatgggc
      601 cacacaaacg cacaaatggt attcaatgtt tacggagcat ggatgaaaga caacaatcac
      661 gaacagatag aacttcttaa cagaagacta tctgaaagtg tcccatgcat gccccataag
      721 aaagcggggt aaaataaaaa cttgcaaaat caattggttt acctttaatc cctgtcacgt
      781 tacgcgcgtg gcagaggcgt tacggattgc tgaaaccgca acggacagac ggcggtcatc
      841 ggctgttcaa cgatgccgat attgaccgga tccgcgagat caaacgctgg atcgacaacg
      901 gcgtgcaggt cagcaaagtt aaaatactgc tcagtaatga aaatgttgat gtgcagaacg
      961 tctggcgcga tcagcaagaa acattactga cttacctgca aag
//
LOCUS       DAOFMX010000266         1003 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.268, whole genome shotgun sequence.
ACCESSION   DAOFMX010000266 DAOFMX010000000
VERSION     DAOFMX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.268"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..87
                     /locus_tag="QBA20_004476"
     CDS             <1..87
                     /locus_tag="QBA20_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDQ6294716.1"
                     /translation="RMVMKGGRWVNFNWESWRPNVSHSRVVK"
     gene            102..524
                     /locus_tag="QBA20_004477"
     CDS             102..524
                     /locus_tag="QBA20_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDQ6294717.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            582..938
                     /locus_tag="QBA20_004478"
     CDS             582..938
                     /locus_tag="QBA20_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294718.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          289 a          200 c          276 g          238 t
ORIGIN      
        1 cgcatggtca tgaaaggcgg gcgctgggtt aattttaact gggagagctg gcgtccgaat
       61 gttagccatt cgagggttgt taagtagttt caggaggatt tatggcgaaa ccttttactc
      121 ccgaacagcg ggaagaactg aagacgcgaa ttgtggaact cgtgcatcag gacggtcggg
      181 tcacgattcg gcagttgtcc gatgaaacag gtatcagtcg tgcgtctgtc ggtcgcttat
      241 gcatagaact ggtcgcaagt ggtgatgtat ataattctgg ctacggctta ttcccgtctg
      301 aacaggctcg caaggactgg caaagcgccc gcaaaaaact ctcgagagta aaggtgagga
      361 aaccggttgt tgttgatccg gaccttatct ggtcattacc tgacggagaa atacgccgct
      421 acgacaggcg cctgaatata atctgtcgcg agtgccggaa gagcgaagct atgcagcgtg
      481 tactggcttt ctatcagggt aattttcagg aggcgatact gtgaatgaaa ttagctatca
      541 ggcttcaatt accgctggca ttcgcatcaa aggagaggag catggaaata aaaccagaag
      601 atgagttaag caatatcgtt ttatttccga taaaagagga tgaccctcgt aatcaggtta
      661 attttcttta taagccatcg gaaagaccat attgccatca cgcctctgta cgggttgacg
      721 aaaaagagcg tcaggtccgc tgtaaaatct gcggtgcggt tgtggagccg tttgactgga
      781 tgctctctgt ggcaaaaaga gaaaccagac tggcagatga tgtaaggcta ttgcgccagg
      841 aggaacagga aaggcggaga aatatagaaa agctgataca gattgagcgt aacgcgaaag
      901 cgcggatacg cagggcgaca aaatccagaa ctgaataatt aaatttagca ctgttaaaaa
      961 tttaatcctt aaccggaggg atttctgcac cctcaaatca tca
//
LOCUS       DAOFMX010000267          983 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.269, whole genome shotgun sequence.
ACCESSION   DAOFMX010000267 DAOFMX010000000
VERSION     DAOFMX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.269"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="QBA20_004479"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="QBA20_004479"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDQ6294719.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          220 c          222 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAOFMX010000268          972 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.270, whole genome shotgun sequence.
ACCESSION   DAOFMX010000268 DAOFMX010000000
VERSION     DAOFMX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.270"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..473)
                     /locus_tag="QBA20_004480"
     rRNA            complement(<1..473)
                     /locus_tag="QBA20_004480"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          223 a          265 c          211 g          273 t
ORIGIN      
        1 aaaggtatta actttactcc cttcctcccc gctgaaagta ctttacaacc cgaaggcctt
       61 cttcatacac gcggcatggc tgcatcaggc ttgcgcccat tgtgcaatat tccccactgc
      121 tgcctcccgt aggagtctgg accgtgtctc agttccagtg tggctggtca tcctctcaga
      181 ccagctaggg atcgtcgcct aggtgagccg ttaccccacc tactagctaa tcccatctgg
      241 gcacatccga tagcaagagg cccgaaggtc cccctctttg gtcttgcgac gttatgcggt
      301 attagctacc gtttccagta gttatccccc tccatcgggc agtttcccag acattactca
      361 cccgtccgcc actcgtcagc gaaacagcaa gctgtttcct gttaccgttc gacttgcatg
      421 tgttaggcct gccgccagcg ttcaatctga gccatgatca aactcttcaa tttaaaagtt
      481 tgatgctcaa agaattaaac ttcgtaatga attacgtgtt cactcttgag acttggtatt
      541 catttatcgt cttgcgacgt taagaatccg tatcttcgag tgcccacaca gattgtctga
      601 taaattgtta aagagcagta ccgcttcgct ttttctcagc ggcgcggggt gtgcataata
      661 cgctttcccg ctacagagtc aagcatttat ttttgctttc tctgccggaa ttctcaggag
      721 aaccccgctg acccggcggc gtgtttgccg ttgttccgtg tcagtggtgg cgcattatag
      781 ggagttattc cggcctgaca agcgaaaaat ataaaaactt tatcgttcgc tcacttttca
      841 ggcaaaacat cttaaatata gtcttttccg tctaacttat agacaaaaac gagccccgaa
      901 gggctaatgc cgatcagtta aggatcagtt gaccgatcca gtggctgtgt aagaatccgg
      961 aaacgctcac tt
//
LOCUS       DAOFMX010000269          959 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.271, whole genome shotgun sequence.
ACCESSION   DAOFMX010000269 DAOFMX010000000
VERSION     DAOFMX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.271"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            60..557
                     /locus_tag="QBA20_004481"
                     /pseudo
     CDS             60..557
                     /locus_tag="QBA20_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            598..789
                     /locus_tag="QBA20_004482"
                     /pseudo
     CDS             598..789
                     /locus_tag="QBA20_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          249 a          240 c          270 g          200 t
ORIGIN      
        1 agcgccagcc ccgtcccgcc cgatagagcg cggcatagcg ggccccggat tacttgcgtg
       61 cgacggcaga agaagccctg aagcgaatca gtgaactgta cgccatcgag gatgaaatac
      121 ggggattacc agagtcagag tgtcttgcag tcaggcagca gcgaagcaaa gcgttactga
      181 cgtcgctgca tgaatggatg gtggagaaga atggcacgct gtcgaaaaaa tccagactgg
      241 gcgaagcatt cagctatgta ctgaatcagt gggacgccct ctgttattac agtgatgacg
      301 gtctggcgga ggcggacaat aacacagcgg aaagagcgct tcgtgcagtc tgtctcggaa
      361 agaaaaatta cgtgttcttc ggtagcgatc acggcggcga gcgtggtgca ctgctgtacg
      421 ggctgatcgg cacctgccgt ctgaacggta tcgatccgga agcgtatctg cgccatattc
      481 tgagcgtact gccggaatgg ccttccaacc gagttgacga cctcctgcca tggaaggtgg
      541 tgctaccatc aggataatcg tcaatacggt gttcggtgaa cgtttacttg gagcatcatg
      601 cttcatgaag aaaaactggc acgtcatcaa cgcaaacagg cgatgtatac ccgaatggca
      661 gccttcccgg cggtgaaaat gttcgaagag tatgacttca cgttcgccac cggagcaccg
      721 cagaagcagc tccagtcgtt acgctcactc agcttcatag agcgcaatga aaacatagtc
      781 ttactgggga catctgacat cacaaatccc cgggtgggga tttgtgtata agagacagcc
      841 catggaggaa ggagatatat cgcagctctg acgactcact ccttatttac ccgggaaata
      901 cttctccagc aatgcataca ggatggcagc ggtttcagca tccattatgc cgtcataat
//
LOCUS       DAOFMX010000270          947 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.272, whole genome shotgun sequence.
ACCESSION   DAOFMX010000270 DAOFMX010000000
VERSION     DAOFMX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.272"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..>947
                     /locus_tag="QBA20_004483"
     CDS             <1..>947
                     /locus_tag="QBA20_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HDQ6294720.1"
                     /translation="KKLDVSCNKLTILTDLPENIKELIARDNFLTHISALPHYLITLD
                     VSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKI
                     LKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQITTIPTNL
                     FRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQMLPQNL
                     HSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRAFSAFLDR
                     LSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFTVAA"
BASE COUNT          282 a          232 c          154 g          279 t
ORIGIN      
        1 ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta cctgaaaata
       61 ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta ccacattatc
      121 taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta ccagacacca
      181 tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta cccttgaatt
      241 taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg ccttctaatc
      301 ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta cctaggagac
      361 tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc caagaaaata
      421 ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc ccgacaaatc
      481 tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta tcagatcaaa
      541 ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct cagtttcgta
      601 tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa aatttacatt
      661 cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc cctcttcccg
      721 aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca caatggactt
      781 ctttttccac cgaggagaat tcccgggcat tctccgcgtt ccttgaccgc ctttccgata
      841 ccgtctctgc acgcaatacc tccggattcc gtgaacaggt cgctgcatgg ctggaaaaac
      901 tcagtgcctc tgcggagctt cgacagcagt ctttcactgt tgctgct
//
LOCUS       DAOFMX010000271          933 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.273, whole genome shotgun sequence.
ACCESSION   DAOFMX010000271 DAOFMX010000000
VERSION     DAOFMX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.273"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(55..243)
                     /locus_tag="QBA20_004484"
     CDS             complement(55..243)
                     /locus_tag="QBA20_004484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294721.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          245 a          181 c          217 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DAOFMX010000272          924 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.274, whole genome shotgun sequence.
ACCESSION   DAOFMX010000272 DAOFMX010000000
VERSION     DAOFMX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.274"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..200
                     /locus_tag="QBA20_004485"
                     /pseudo
     CDS             1..200
                     /locus_tag="QBA20_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..>924
                     /locus_tag="QBA20_004486"
     CDS             300..>924
                     /locus_tag="QBA20_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDQ6294722.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          293 a          217 c          207 g          207 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatcggaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aagg
//
LOCUS       DAOFMX010000273          921 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.275, whole genome shotgun sequence.
ACCESSION   DAOFMX010000273 DAOFMX010000000
VERSION     DAOFMX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.275"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..921
                     /locus_tag="QBA20_004487"
                     /pseudo
     CDS             1..921
                     /locus_tag="QBA20_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          217 a          221 c          288 g          195 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgctggactg
      481 ctgtgatcgt gaggcactgc actgggcggt gactaccggc ggcttcaaca gtgaaacagt
      541 acaggacgtc atgctgggag cggtggaacg ccgcttcggc aacgatcttc cgtcgtctcc
      601 agtggagtgg ctgacggata atggttcatg ctaccgggct aatgaaacac gccagttcgc
      661 ccggatgttg ggacttgaac cgaagaacac ggcggtgcgg agtccggaga gtaacggaat
      721 agcagagagc ttcgtgaaaa cgataaagcg tgactacatc agtatcatgc ccaaaccaga
      781 cgggttaacg gcagcaaaga accttgcaga ggcgttcgag cattataacg aatggcatcc
      841 gcatagtgcg ctgggttatc gctcgccacg ggaatatctg cggcagcggg cttgtaatgg
      901 gttaagtgat aacagatgtc t
//
LOCUS       DAOFMX010000274          833 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.276, whole genome shotgun sequence.
ACCESSION   DAOFMX010000274 DAOFMX010000000
VERSION     DAOFMX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.276"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..326
                     /locus_tag="QBA20_004488"
     CDS             <1..326
                     /locus_tag="QBA20_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDQ6294723.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="QBA20_004489"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="QBA20_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDQ6294724.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          253 a          183 c          155 g          242 t
ORIGIN      
        1 ttagtgaaac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga gtg
//
LOCUS       DAOFMX010000275          828 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.277, whole genome shotgun sequence.
ACCESSION   DAOFMX010000275 DAOFMX010000000
VERSION     DAOFMX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.277"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..683)
                     /locus_tag="QBA20_004490"
     CDS             complement(<1..683)
                     /locus_tag="QBA20_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HDQ6294725.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLL"
BASE COUNT          251 a          136 c          173 g          268 t
ORIGIN      
        1 tgtaacaaag tttgcattat tctgggagat agttgatttt ctctaagatt gacaacgcta
       61 tcagaactga ggccaagtat gctttccgga attgccgtga tccggttctg attaagccag
      121 aatattctta taatatgatc atctctttct ggaaaatctg gtattacttc caaagcattt
      181 ctggctgcat caagtaattc caatgacatt ggtaacctcg gaagtacaga caggtgattg
      241 tcacttacat taatatattc tagtgacgca ggtaattcag gaagtgcgaa caaatggtta
      301 tcactcacat ttatatattc cagcgacact ggtaattcag gaagtgcaga taattgatta
      361 ctgcttgcat tcagctcttt caatgccctt ggtagctcgg ggagcattga tagttggtta
      421 ttgcttacat taatttcatc aagattgtca ggcaatcgtg gtagagatct gagacctaaa
      481 caagataagt ccaacgatgt ttcactgttc tccagacata attggagccg ggtaaaagca
      541 gtttccctgt tttctccgga aatgctgttt ttagtccatt caacccattc ggagagataa
      601 ttattgtgaa cattgtcgat tgatgtagtt ctgtaaaaag agacgtttcc agtggatagg
      661 gggggattat ttacaggaag cataataacc tcgcagaagg atatcctgat aaaatgtgat
      721 actggggaat aataagctga aataaaatgt ttcaggattg aaatttattg ttttcatttt
      781 aaggaagtga tgttgattta actgtaaaaa aacgatacta ttttctta
//
LOCUS       DAOFMX010000276          792 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.278, whole genome shotgun sequence.
ACCESSION   DAOFMX010000276 DAOFMX010000000
VERSION     DAOFMX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.278"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(8..84)
                     /locus_tag="QBA20_004491"
     tRNA            complement(8..84)
                     /locus_tag="QBA20_004491"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Arg,seq:tct)
     gene            complement(86..162)
                     /locus_tag="QBA20_004492"
     tRNA            complement(86..162)
                     /locus_tag="QBA20_004492"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(126..128),aa:Ile,seq:cat)
     gene            complement(352..>792)
                     /locus_tag="QBA20_004493"
     CDS             complement(352..>792)
                     /locus_tag="QBA20_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HDQ6294726.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          197 a          221 c          155 g          219 t
ORIGIN      
        1 tcggcgctgg cccaccaccg aggactcgaa cctcgcaccg tcaacttaga aggttgatgc
       61 tctatccgga tgagctagtg gtggttggtg gcccttgctg gacttgaacc agcgacctgg
      121 cgattatgag tcgctcgctc tgaccaactg agctaaaggg ccggaggcag aataataacc
      181 atatgtcatc acatctgcaa actcatctga tcaccagcac gtttaacgtc ctgtgccgtt
      241 tttcaggtat aaaaaaaacc gcataaagcg ggctctttca aatgtccatg tctgctattc
      301 gcctcgcggt acagctttgc gaagcgtaca agaattgaag cagtttttac gtcaaaaagc
      361 aataactttt ttctctatac caaaagccat aaccattggt ttgtacaaaa taaattctgc
      421 cacctttagc caatgctcaa tgcgtctttc acaggttctt aaactccatt tcgggtgtgc
      481 atcattcagc agttcagcca ttttgcattt ggtcatcccc cgcccctcat accgttgccg
      541 aaggacgcta atcaatcctg gatgctctgc cagcacctca cttatgaccc gatcaataca
      601 taacgcctct gcatcagtac aatgcgccag ccagctcttt tgcttaccgt taatcatatc
      661 ccgcaaaaaa gcctcaagtt cagacttgtt cagacctgct tttttcatcc tccggagcgc
      721 ctcgttaatt gccgtttttg tcagcttttt agaggtcaac atcaggttaa acatattccc
      781 cgtcttaccg cc
//
LOCUS       DAOFMX010000277          762 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.279, whole genome shotgun sequence.
ACCESSION   DAOFMX010000277 DAOFMX010000000
VERSION     DAOFMX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.279"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            206..>762
                     /locus_tag="QBA20_004494"
     CDS             206..>762
                     /locus_tag="QBA20_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HDQ6294727.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLHVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          264 a          145 c          143 g          210 t
ORIGIN      
        1 ggtgacatca ccccccggat catggacgac tgggggcagt aatcatgccg ggtctggctg
       61 gctaacaccc cattataagg ggtacgaata aagtaacggg ggaagcaata cctggagaaa
      121 gagtacaaag caaactgtat taaacttaat aagttatatt gtatttcact agagttctta
      181 tcgttgataa gaaattctgg ttataatgat taaatcaacg aatatacagg taatcggttc
      241 tggtattatg catcaaataa acaacataca ctcgttaact ctattttctt tgcatgtgag
      301 tttgagtcca tcttgtaatg aatattattt aaaagtctgg agtgaatggg aaaagaacgg
      361 taccccaggc gagcaacgca atatcgcctt caataggcta aaaatatgtt tgcaaaatca
      421 aggggcggaa ttaaatctat ctgagttaga tttaaaaaca ttacctgatt taccgcctca
      481 gataacaaca ctggaaataa gaaaaaacct attaacatat ctccctgatt tcccaccaat
      541 gcttaaggta atacatgctc aatttaatca actggaaagc ttacctgcct tacccgagac
      601 gttagaagag cttaatgtgg gtgataacaa gataaaagaa ttaccatatc ttcctgaaac
      661 tctaactcat ttacggatcc ataataaccg attgcatatt ctgccactat tgccaccgga
      721 gctaaaatta ctgatagttt ctggaaacag attagacagc at
//
LOCUS       DAOFMX010000278          762 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.280, whole genome shotgun sequence.
ACCESSION   DAOFMX010000278 DAOFMX010000000
VERSION     DAOFMX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.280"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            60..>762
                     /locus_tag="QBA20_004495"
     CDS             60..>762
                     /locus_tag="QBA20_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDQ6294728.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQVSCK
                     GSVDSTITDLLEKALN"
BASE COUNT          262 a          109 c          159 g          232 t
ORIGIN      
        1 tttttttgtg agtaaaattt gtaatgctta gacgttctta ttaactcaag gagttcgtca
       61 tgggcaaaat atcagatttg aattattctc aacacattac attagccgac aattttaaac
      121 aaaaaaatga agccttagat acctggtatg tgggaatgaa tgattttgcc cgaattgccg
      181 gggggcagaa tagcagaagc aatattcttt ctcctagagc atttttggag tttttggcaa
      241 agatatttac cctgggttat gtggatttta gcaaacgctc caacgaagcg ggtagaaata
      301 tgatggctca tattgagttc tcatcttatt ctaaagatac tgatggcaat gaaaaaatga
      361 agttttacat gaataatcct gaaggggagc gagcggattt atctaaggtg aagatagaaa
      421 ttacacttgc ctcagcttct actaaaggga ttcgtgaagg acatacagtc attatattta
      481 aacaatctga tggttcgact aatcgttatg aagggaagtc ttttgaaaga aaagatgata
      541 gttcattaca cctgattact aacaaggttc tggcgtgtta ccaacgcgaa gctaacaaga
      601 aaatagcgcg tctattaaat aatcatcaga agttaaataa tatacaggag ttaaatgatt
      661 cgcaggagtt aaataattca cagaagttaa ataattcgca ggtgagttgt aaaggttcag
      721 ttgattctac gattacggat ttattagaaa aagcattgaa ta
//
LOCUS       DAOFMX010000279          758 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.281, whole genome shotgun sequence.
ACCESSION   DAOFMX010000279 DAOFMX010000000
VERSION     DAOFMX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.281"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..>758
                     /locus_tag="QBA20_004496"
     CDS             <1..>758
                     /locus_tag="QBA20_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDQ6294729.1"
                     /translation="RILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFELD
                     LEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGTD
                     LFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDG
                     FSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDV
                     VNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          183 g          209 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcggtgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgtagca
//
LOCUS       DAOFMX010000280          758 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.282, whole genome shotgun sequence.
ACCESSION   DAOFMX010000280 DAOFMX010000000
VERSION     DAOFMX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.282"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..306
                     /locus_tag="QBA20_004497"
     CDS             <1..306
                     /locus_tag="QBA20_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294730.1"
                     /translation="REENEFTDWFSLWGPWHAVLKRTEADRWALAEEQKYEMLENEYP
                     QRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLPENGSQLHHS"
     gene            complement(486..>758)
                     /locus_tag="QBA20_004498"
     CDS             complement(486..>758)
                     /locus_tag="QBA20_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HDQ6294731.1"
                     /translation="RTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSMP
                     ELEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
BASE COUNT          172 a          224 c          205 g          157 t
ORIGIN      
        1 cgtgaagaga atgaatttac ggactggttc tccctctggg gaccatggca tgctgtactg
       61 aagcgtacgg aagctgaccg ctgggcgctg gcagaagagc agaaatatga gatgctggag
      121 aatgagtacc ctcagagggt ggctgaccgg ctgaaagcat caggtctgag cggtgatgcg
      181 gatgcggaga gggaagccgg tgcacaggtg atgcgtgaga ctgaacagca gatttaccgt
      241 cagctgactg acgaggtact ggccctgcga ttgcctgaaa acggctcaca actgcaccat
      301 tcataatcac atcgcataaa ccacagaccg gactgactcc ggaaaaacag aggcccgccc
      361 ccgggcctcc ccggattcat ccgtttctct gttcagcctg accgcacacc ccggcggccg
      421 gatgacagac tccgcctcgg taagcaaagc ggtcttctgt gattccgcca gttgcggctt
      481 attcattact taacgtcaaa cgcccgaatt gaagccaaat catccagact gctcagctcc
      541 tctttcatct cccgctgacg gcgataaatc tcatcgttgc gatcgacctg cgcctgcacc
      601 attgctgccg ccagttcttc cagttccggc atcgacagtt tcacctgctg attatccgca
      661 tcactccaca ccatatgtgt ttgtgccgcg acagattttg ccagcatgac caccggggac
      721 aggcgggcca ctgacgaggc gtcagcgttc cacgtgcg
//
LOCUS       DAOFMX010000281          745 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.283, whole genome shotgun sequence.
ACCESSION   DAOFMX010000281 DAOFMX010000000
VERSION     DAOFMX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.283"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..>745)
                     /locus_tag="QBA20_004499"
     CDS             complement(<1..>745)
                     /locus_tag="QBA20_004499"
                     /inference="COORDINATES: protein motif:HMM:NF012714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294732.1"
                     /translation="TMALSSPAFAGKAVKLSPAASEVLGSGRVTMRKTVAKPKGPSGS
                     PWYGSDRVKYLGPFSGESPSYLTGEFPGDYGWDTAGLSADPETFARNRELEVIHSRWA
                     MLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSDGGLDYLGNPSLVHAQSILAIWAT
                     QVILMGAVEGYRVAGNGPLGEAEDLLYPGGSFDPLGLATDPEAFAELKVKELKNGRLA
                     MFSMFGFFVQAIVTGKGPIENLADHLADPV"
BASE COUNT          171 a          217 c          194 g          163 t
ORIGIN      
        1 taactggatc ggccaaatgg tcagcaaggt tctctatcgg tcccttacca gtgacgatgg
       61 cttgaacgaa gaatccaaac atagagaaca tagccaatct tccgttcttg agctccttca
      121 ccttcaactc agcgaatgcc tctgggtcgg tagccaaacc caatgggtcg aagctgccac
      181 cggggtaaag caagtcctcg gcctctccca atggcccatt tcctgcgact ctgtagcctt
      241 caacggctcc catcaaaata acttgtgtgg cccaaatggc caaaatgctc tgagcgtgaa
      301 ccaagctagg gtttcccaag taatcgagcc ctccatcgct gaagatctgt gaaccggcct
      361 tgaaccaaac cgcctctccg aacttgactc cgtttctagc caaaagctca gggaagacgc
      421 agcctagggc tccgagcatg gcccacctgc tgtggataac ttctagttca cggttccttg
      481 cgaatgtctc ggggtcagct gaaagtccgg cggtgtccca tccgtagtct ccggggaact
      541 ctccggtaag gtagctcggt gattcgccag agaatggacc caagtacttg acacggtcag
      601 atccgtacca tgggctgcct gatgggccct ttggcttggc aacagtcttc ctcattgtca
      661 cacggccgct tccaaggact tcagatgccg cgggggaaag cttgacggcc ttaccggcga
      721 aggcagggga ggagagagcc attgt
//
LOCUS       DAOFMX010000282          697 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.284, whole genome shotgun sequence.
ACCESSION   DAOFMX010000282 DAOFMX010000000
VERSION     DAOFMX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.284"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..529
                     /locus_tag="QBA20_004500"
                     /pseudo
     CDS             1..529
                     /locus_tag="QBA20_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            593..>697
                     /locus_tag="QBA20_004501"
     CDS             593..>697
                     /locus_tag="QBA20_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDQ6294733.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDIT"
BASE COUNT          194 a          143 c          167 g          193 t
ORIGIN      
        1 tgattgtgct gatcgtgcgg atgattacta ccatgaccgt ctggcgctga ctgttgataa
       61 ttcaacgatc gacgacaact acgaacacta cacatataac ggcacttaca ataatgccgc
      121 tgatactcat gttgtagacg tttacaacat tggtacggct attactctgg atcaggaagt
      181 tgatctgtcc atcactaata actctcacgt tgcgggtatt acgttgactc agggttatga
      241 gtgggaagat attgacgaca acactgtcag cactggcgta aacagcagcg aagtgtttaa
      301 taacaccatc actgttaaag attctaccgt aacctctggt tcatggactg atgaaggtac
      361 tactggttgg ttcggtaata caggtaatgc cagcgattat agtggtaaat ctaactttgt
      421 caccgtagat actgacggag atggtgttgc tgatagcact attgcaagct gggatgatgt
      481 tgcattagct gtagttgcac atccgaatgt tgataatgct atgcagacct gaatcgccac
      541 gggtttaaca gacacctcag agtcatttaa gatggcttaa agagaggtgc ccatgagcgg
      601 taagcgttat cccgaagagt ttaaaactga agcagtcaaa caggttgttg atcgcggtta
      661 ttctgttgcc agcgttgcaa cacgtctcga tatcacc
//
LOCUS       DAOFMX010000283          684 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.285, whole genome shotgun sequence.
ACCESSION   DAOFMX010000283 DAOFMX010000000
VERSION     DAOFMX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.285"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..>684
                     /locus_tag="QBA20_004502"
     CDS             <1..>684
                     /locus_tag="QBA20_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDQ6294734.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRS"
BASE COUNT          214 a          121 c          164 g          185 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gctt
//
LOCUS       DAOFMX010000284          672 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.286, whole genome shotgun sequence.
ACCESSION   DAOFMX010000284 DAOFMX010000000
VERSION     DAOFMX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.286"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(151..>672)
                     /locus_tag="QBA20_004503"
     CDS             complement(151..>672)
                     /locus_tag="QBA20_004503"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012329.1,HMM:NF023756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-1,5-bisphosphate carboxylase small
                     subunit"
                     /protein_id="HDQ6294735.1"
                     /translation="SATMVASPAQATMVAPFNGLKSSAAFPATRKANNDITSITSNGG
                     RVNCMQVWPPIGKKKFETLSYLPDLTDSELAKEVDYLIRNKWIPCVEFELEHGFVYRE
                     HGNSPGYYDGRYWTMWKLPLFGCTDSAQVLKEVEECKKEYPNAFIRIIGFDNTRQVQC
                     ISFIAYKPPSFTG"
BASE COUNT          203 a          143 c          176 g          150 t
ORIGIN      
        1 gagaatccga tagaatatgt ctcgcaaacc ggaaaacaaa cgttaagtcc ggaaattaaa
       61 acccaagaaa gaagaaaaaa gcagaaaaac aagggataaa atcaaagatg ggggataaag
      121 ttttgaggtt tacacaaaag caaagggaaa ttaaccggtg aagcttggtg gcttgtaggc
      181 aatgaaactg atgcactgga cttgacgggt gttgtcgaat ccgatgatcc taatgaaggc
      241 attggggtac tccttcttgc actcttccac ttccttcaac acttgagcgg agtcggtgca
      301 accgaacaag ggaagcttcc acattgtcca gtaccgtcca tcatagtatc cgggtgagtt
      361 accgtgctca cggtacacaa atccgtgctc caactcgaat tcaacacaag gaatccactt
      421 gttgcggata aggtagtcaa cttccttagc caattcggaa tcggtaaggt caggaaggta
      481 agagagagtc tcaaacttct tctttccaat cggaggccac acctgcatgc agttaactct
      541 tccgccgttg cttgtgatgg aagtaatgtc gttgttagcc ttgcgggtgg ctgggaaggc
      601 agcggaggac ttaagtccgt tgaaaggagc gaccatagtg gcctgagccg gagaggcaac
      661 catagtagcg ga
//
LOCUS       DAOFMX010000285          659 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.287, whole genome shotgun sequence.
ACCESSION   DAOFMX010000285 DAOFMX010000000
VERSION     DAOFMX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.287"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(151..657)
                     /locus_tag="QBA20_004504"
     CDS             complement(151..657)
                     /locus_tag="QBA20_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDQ6294736.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          173 a          161 c          145 g          180 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc ggaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaatct
//
LOCUS       DAOFMX010000286          649 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.288, whole genome shotgun sequence.
ACCESSION   DAOFMX010000286 DAOFMX010000000
VERSION     DAOFMX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.288"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            20..493
                     /locus_tag="QBA20_004505"
     CDS             20..493
                     /locus_tag="QBA20_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDQ6294737.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          215 a          117 c          130 g          187 t
ORIGIN      
        1 gggagatgaa ccctatgcaa tgtcctggtt aatatctcag tgtgctcatc agtgcactga
       61 taataaaaag acggagacag atgccattta cgataaagtt cggagttctt atttgctttc
      121 atgtattctg aaaaaaaaca aaaatgttgg gcttattctt catgcaccat catttgtttc
      181 tgtgtctgaa aaaatcgcca gaattgtgat ggcaaattac tcccgtaact ggagtaattc
      241 cgagttggcc agtgcggttt taatgagtga atcttcttta aaaagaagaa tgtacaaaga
      301 ggtcggttct ataagtacat ttgtacataa aataaagctt acagaagcta tcaggaaatt
      361 acgaaggaca aatactccca tttctgtcat atcatctgag ttaggttaca gttcaccatc
      421 gtatttttct aaagtcttct ttaaatattt aaagacatac cctcaaaaca taaggaagaa
      481 aaatggtcgg taatgtcacc aattaggagg acttgcagag ccgctttttt acgatactga
      541 gatcaaacag acataatcta tgcggagatt tacagcaggg catggttcac aaatctgaca
      601 gcgatgaatt atcggcactg cgggcagaaa atgctcgaat catcaagcc
//
LOCUS       DAOFMX010000287          644 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.289, whole genome shotgun sequence.
ACCESSION   DAOFMX010000287 DAOFMX010000000
VERSION     DAOFMX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.289"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..>644
                     /locus_tag="QBA20_004506"
     rRNA            <1..>644
                     /locus_tag="QBA20_004506"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          164 a          152 c          193 g          135 t
ORIGIN      
        1 acggccgcaa ggttaaaact caaatgaatt gacgggggcc cgcacaagcg gtggagcatg
       61 tggtttaatt cgatgcaacg cgaagaacct tacctggtct tgacatccac ggaagttttc
      121 agagatgaga atgtgccttc gggaaccgtg agacaggtgc tgcatggctg tcgtcagctc
      181 gtgttgtgaa atgttgggtt aagtcccgca acgagcgcaa cccttatcct ttgttgccag
      241 cggtccggcc gggaactcaa aggagactgc cagtgataaa ctggaggaag gtggggatga
      301 cgtcaagtca tcatggccct tacgaccagg gctacacacg tgctacaatg gcgcatacaa
      361 agagaagcga cctcgcgaga gcaagcggac ctcataaagt gcgtcgtagt ccggattgga
      421 gtctgcaact cgactccatg aagtcggaat cgctagtaat cgtggatcag aatgccacgg
      481 tgaatacgtt cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgcaaaa
      541 gaagtaggta gcttaacctt cgggagggcg cttaccactt tgtgattcat gactggggtg
      601 aagtcgtaac aaggtaaccg taggggaacc tgcggttgga tcac
//
LOCUS       DAOFMX010000288          638 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.290, whole genome shotgun sequence.
ACCESSION   DAOFMX010000288 DAOFMX010000000
VERSION     DAOFMX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.290"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            90..155
                     /locus_tag="QBA20_004507"
                     /pseudo
     CDS             90..155
                     /locus_tag="QBA20_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            383..631
                     /locus_tag="QBA20_004508"
     CDS             383..631
                     /locus_tag="QBA20_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HDQ6294738.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          132 c          147 g          157 t
ORIGIN      
        1 ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
       61 actgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
      121 tgctttgtga tattaatcct gtcacgttac gcgcgtgacg caacattttt aaaaatcgac
      181 gaagatcgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg
      241 acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca
      301 taaacaatat caggaattga cactggttat ccatacagat aaaaataaca cctgtacata
      361 actacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcac
      421 taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac gcatcagtaa
      481 acgttatgac gatgtagagg tgatcgtaaa agccaccagc aacgatggcc tttctgttac
      541 acgcaccgca gataaggatt ctgcaaaaac atttgttcag gagactctga aagatacctg
      601 ggagtctgct gacgagtggt ttgttcacta ataaacac
//
LOCUS       DAOFMX010000289          630 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.291, whole genome shotgun sequence.
ACCESSION   DAOFMX010000289 DAOFMX010000000
VERSION     DAOFMX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.291"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..112)
                     /locus_tag="QBA20_004509"
     CDS             complement(<1..112)
                     /locus_tag="QBA20_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HDQ6294739.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYI"
     gene            complement(109..399)
                     /locus_tag="QBA20_004510"
     CDS             complement(109..399)
                     /locus_tag="QBA20_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDQ6294740.1"
                     /translation="MVDLLKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(399..>630)
                     /locus_tag="QBA20_004511"
     CDS             complement(399..>630)
                     /locus_tag="QBA20_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDQ6294741.1"
                     /translation="FREWVAIQAGFYTEHFYPDGSRGCRAKSIAFANMDETEFQQVYK
                     SVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
BASE COUNT          149 a          181 c          136 g          164 t
ORIGIN      
        1 caatatacga ccagcgcccc cacatacgta gttttccctg aatccagaca ctttccagcg
       61 tggtgagacg aaggtgttct ccgctttttc ctgtattcgt tgggtaaatc ataaatatcc
      121 ctcctttctc cagatttctt gtgtgcgaaa aacaccttct gcatgcatca ggcgtaattc
      181 ttctttggtg taatcgctgg tttttacccg cccgtcgatt aaatcgtggc acgagctaca
      241 ggcaatcgct gcctgcatat cgtgtggctt tatcgctgtt ccgcacgttc ccgccagtcg
      301 gtaatgcgcc agcacagacg tttccggatc gtgattgcag tagccaggaa ttctgacggt
      361 gcacatctgc ccccgcgccg ctttaagtaa atccaccatt acgcaaactc cagcagctgc
      421 gcggccacat tttcgacttg ttccggagag gaaaatttac ggaacagaat ccagttccac
      481 agcacattca gtacagattt ataaacctgc tgaaactcgg tttcgtccat attcgcaaac
      541 gcgatagatt tcgcccgaca cccacggcta ccgtccggat aaaaatgctc ggtgtaaaat
      601 ccggcctgaa tggctaccca ctcgcggaaa
//
LOCUS       DAOFMX010000290          621 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.292, whole genome shotgun sequence.
ACCESSION   DAOFMX010000290 DAOFMX010000000
VERSION     DAOFMX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.292"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            78..621
                     /locus_tag="QBA20_004512"
                     /pseudo
     CDS             78..621
                     /locus_tag="QBA20_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          144 a          158 c          165 g          154 t
ORIGIN      
        1 gatggggcgc ttacaaacga atcggaaaat cgatactgga aaaactctaa ctcggtggcc
       61 agtttttgtt gaccacttca ggtcaggaga ttgtcgtcga accgattgaa caggctattc
      121 tcatctctga gcagactaag ctgtgggacg ccaagtcatc caccggattc actcagggca
      181 cacttcagca ggacgctatg aacatctgtg agaatgtggt ccgtaccatc aacgctcgca
      241 tggtcgacgt tctggaatcc tctaagctgc tgaagaccgt agagcttcct gtcctgactg
      301 gtagcctgac cgctaaggca gacgctatca tggacgctct gtatgagaac accgagcctt
      361 ctttcggttc tgaggtgtct gactacggta ttatcgccca tgagagccaa ctgaaagcac
      421 tgtcccgtct ggctgctaaa cagggcttct ctggtgaaga cgctatcgtg gatatgctgg
      481 gtaccgacat cgcgtattac aacggtgagg acaaaggtgt gttcatgttg gctaaacgct
      541 tcacggccct gagcttcggc tgcttccgtc acgacggtga gaatattacg gtcgtccttt
      601 cacgtgatgg cgactctcag t
//
LOCUS       DAOFMX010000291          619 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.293, whole genome shotgun sequence.
ACCESSION   DAOFMX010000291 DAOFMX010000000
VERSION     DAOFMX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.293"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..>619
                     /locus_tag="QBA20_004513"
     CDS             <1..>619
                     /locus_tag="QBA20_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HDQ6294742.1"
                     /translation="RQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRT
                     PDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLI
                     EQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKL
                     KTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT          162 a          176 c          180 g          101 t
ORIGIN      
        1 ccgccagcgc ctgggaagtg aggctgtccg ccgcgtgttc acgaaaacag cgcagctctg
       61 gcataacgcc acgccgcatc cgcactggtg cggcctgacc ctgctggcca tcgatggtgt
      121 gttctggcgc acaccggata caccagagaa cgatgcagcc ttcccccgcc agacacatgc
      181 cgggaacccg gcgctctacc cgcaggtcaa aatggtctgc cagatggaac tgaccagcca
      241 tctgctgacg gctgcagcct tcggcacgat gaagaacagc gaaaatgagc ttgctgagca
      301 acttatagaa caaaccggcg ataacactct gacgttaatg gataaaggtt attactcact
      361 gggactgtta aatggctgga gcctggcggg agaacaccgc cactggatga tacctctcag
      421 aaagggagcg caatatgaag agctcagaaa actgggtaaa ggcgatcatc tggtgaagct
      481 gaaaaccagc ccgcaggcac gaaaaaagtg gccgggactg ggaaatgaag tgacagcccg
      541 cctgctgacc gtgacgcgca aaggaaaagt ctgccatctg ctgacgtcga tgacggacgc
      601 catgcgcttc cccggagga
//
LOCUS       DAOFMX010000292          603 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.294, whole genome shotgun sequence.
ACCESSION   DAOFMX010000292 DAOFMX010000000
VERSION     DAOFMX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.294"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..127
                     /locus_tag="QBA20_004514"
     CDS             <1..127
                     /locus_tag="QBA20_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HDQ6294743.1"
                     /translation="VTCKRNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            complement(161..364)
                     /locus_tag="QBA20_004515"
                     /pseudo
     CDS             complement(161..364)
                     /locus_tag="QBA20_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            464..>603
                     /locus_tag="QBA20_004516"
     CDS             464..>603
                     /locus_tag="QBA20_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDQ6294744.1"
                     /translation="MRMNMSDFATSFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LK"
BASE COUNT          156 a          146 c          138 g          163 t
ORIGIN      
        1 ggtcacctgc aaacggaacg aagtggagcc ggaggactgg ttgcgcgaag tgatcgagaa
       61 gctcaatgac tggccgtcga accaagtgca tgaactgctg ccctggaact tctcgtctgt
      121 aaaataatcc ttacgctacg tacttctcgg ggcgcttacc tggagaataa cgtttatcca
      181 tattcttcca gtctggttca tcttccgacg tcatcaccgg gttgaaatta cgccgatata
      241 aatcgagttc cgtcacctgt attttccgct ccattgcccg ctgatggatc gcatcagcaa
      301 tatttgcggt caatgaatca tgacgaggat gggcccatac caaataaata cgttcagact
      361 gcataatttt acctccacgt taagttctgg agcgcacttt acgcaggcgg cataatcctg
      421 taaacgtgat gattcaggat gagttaatga aggattttca ctaatgcgga tgaatatgtc
      481 tgactttgcc acttcctttg ccgtggcccg taatcaaagc tttcgtgcag caggcgatga
      541 gttaggctta tcctcgtccg ccattagcca tagtattaaa acactggaac aacgtcttaa
      601 aat
//
LOCUS       DAOFMX010000293          592 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.295, whole genome shotgun sequence.
ACCESSION   DAOFMX010000293 DAOFMX010000000
VERSION     DAOFMX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.295"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..>592
                     /locus_tag="QBA20_004517"
     CDS             <1..>592
                     /locus_tag="QBA20_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDQ6294745.1"
                     /translation="SCCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQ
                     FWLESGCVYGYRKIHLDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIV
                     SPNRLQRQFNPDAPDERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDI
                     VLNALLMAVWRRNPEKQVLVHSDQGSQYTSHEWQSFL"
BASE COUNT          138 a          150 c          169 g          135 t
ORIGIN      
        1 ctcctgttgc tggcctgttc gcctgctctg tcgggtgctg gatgttcatc ccagtggttt
       61 ttacgcctgg cttcagcagc cgcattcaca acgccatcag gcagacctga gactgacagg
      121 acagattaaa cagttctggc tggaatcggg atgcgtctat ggttatcgca aaatccatct
      181 ggatctgcgt gacagcgggc aacagtgcgg agtaaacaga gtctggagac tgatgaaacg
      241 tgtcggaata aaggctcagg tcggataccg aagcccgcgg gcacgtaaag gcgaggccag
      301 tatcgtgtca cccaacaggc tccagcgaca gttcaatccg gatgctcctg atgagcgttg
      361 ggtaacggac ataacctaca tcaggaccca cgaaggctgg ctgtatcttg ccgttgttgt
      421 tgatctgttc tcacgcaaaa ttatcggctg gtccatgcaa tcccggatga caaaggacat
      481 tgtcctgaac gcactgctga tggctgtatg gcggcgtaat cccgaaaaac aggtgctggt
      541 tcattcggat cagggcagtc agtacacaag ccatgagtgg cagtcgttcc tg
//
LOCUS       DAOFMX010000294          566 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.296, whole genome shotgun sequence.
ACCESSION   DAOFMX010000294 DAOFMX010000000
VERSION     DAOFMX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.296"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..297
                     /locus_tag="QBA20_004518"
     CDS             <1..297
                     /locus_tag="QBA20_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDQ6294746.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="QBA20_004519"
     CDS             complement(405..>566)
                     /locus_tag="QBA20_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294747.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAOFMX010000295          554 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.297, whole genome shotgun sequence.
ACCESSION   DAOFMX010000295 DAOFMX010000000
VERSION     DAOFMX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.297"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..>554)
                     /locus_tag="QBA20_004520"
     CDS             complement(<1..>554)
                     /locus_tag="QBA20_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HDQ6294748.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIKW"
BASE COUNT          134 a          120 c          135 g          165 t
ORIGIN      
        1 cccatttaat atcaatctgt tgaaggtgta ttattacatc agtagcatat tccagactgc
       61 cttcctggac gatctgatcc ataacatcct cacgcacttt atatcttgtg catttcattt
      121 caagtgccgt gagtacagcc attactggtg atggtagctg tgttgttttc aggccgtcga
      181 tctgactgat cgcttgggcg acggtgtttt gccattctaa agaacaggtg ctgaagtcaa
      241 atgtggctgt tttattatcc cgttgcgaca cgctacaggc taagcgacgt aattgggtca
      301 atttgtctgc ttgctgaaca ggaactccgg gaaagcggcg atgacttaac gtggtgtgta
      361 caacttcggg cggaaaaacg acagcaatat ctttcgccag ccaaggtaaa acagcatctg
      421 tatttttgtc agccgcacgg gtttctgcta cgaaagaggg ttgttcctga ataatggtgg
      481 ataagtccgc taccttgaga gagtccgtta ccgtttggga aggttgcaaa gaattatctt
      541 tcgcttgctc caca
//
LOCUS       DAOFMX010000296          549 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.298, whole genome shotgun sequence.
ACCESSION   DAOFMX010000296 DAOFMX010000000
VERSION     DAOFMX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.298"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..>549
                     /locus_tag="QBA20_004521"
     CDS             <1..>549
                     /locus_tag="QBA20_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HDQ6294749.1"
                     /translation="RGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLE
                     RQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRS
                     LKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT          128 a          133 c          178 g          110 t
ORIGIN      
        1 gagaggctac cagatgggac gctggcttgc tggcaggctc atgaaagagc tggggctggt
       61 cagctgtcag cagccgactc accggtataa acgtggtggt catgaacatg ttgctatccc
      121 taactacctt gaaaggcagt tcgccgtgac cgagccaaat caggtgtggt gcggtgatgt
      181 gacctatatc tggacgggta agcgctgggc gtacctcgcc gttgttctcg acctgttcgc
      241 aagaaaacca gtgggctggg ccatgtcgtt ctcgccggac agcaggctca ccatgaaagc
      301 gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg gtgatgttcc acagcgatca
      361 gggcagtcat tatacgagca ggcagttccg gcagttattg tggcgatacc agatcagaca
      421 gagtatgagc cggcgcggaa actgctggga taacagccca atggaacgct tcttcaggag
      481 tctgaagaac gaatggatgc cggtggtggg ttacgtaagc ttcagcgagg cagctcacgc
      541 cataacgga
//
LOCUS       DAOFMX010000297          536 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.299, whole genome shotgun sequence.
ACCESSION   DAOFMX010000297 DAOFMX010000000
VERSION     DAOFMX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.299"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            82..>536
                     /locus_tag="QBA20_004522"
     CDS             82..>536
                     /locus_tag="QBA20_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDQ6294750.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQT"
BASE COUNT          125 a          157 c          131 g          123 t
ORIGIN      
        1 tttgccggga ccgtctgtaa cgtggatgcc ggtacctgct ccccgtggtt atctggttaa
       61 cccatataca agggagacag aatgaccgaa tccagcgatt acgaatccgt ccaggtcttt
      121 atcggcgttg atgtcggtaa agatacgcat cacgctgttg ccattaatcg ttcaggtaaa
      181 cgcctgttcg ataaagcatt acccaacgac gaaaacaaac tcaggtcgct aatatctgac
      241 ctgaaacaac atggtcagat actgctggtt gttgatcagc cagctaccat cggtgcgtta
      301 cctgtcgccg ttgcccgctc agaaggagtc cttgtcggat acctccctgg actggccatg
      361 cgccgcatag ccgacttaca cgccggtgaa gctaaaactg atgctcgtga cgctgccatc
      421 attgccgaag ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag
      481 caaatcgccg aactctccat gctctgcggc ttcgatgatg atcttgccgc acagac
//
LOCUS       DAOFMX010000298          507 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.300, whole genome shotgun sequence.
ACCESSION   DAOFMX010000298 DAOFMX010000000
VERSION     DAOFMX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.300"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            155..340
                     /locus_tag="QBA20_004523"
                     /pseudo
     CDS             155..340
                     /locus_tag="QBA20_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          143 a          125 c          128 g          111 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgc aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaaggg gatatatcgc
      421 agctctgacg actcactcct tatttacccg ggaaatactt ctccagcaat gcatacagga
      481 tggcagcggt ttcagcatcc attatgc
//
LOCUS       DAOFMX010000299          496 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.301, whole genome shotgun sequence.
ACCESSION   DAOFMX010000299 DAOFMX010000000
VERSION     DAOFMX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.301"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            210..>496
                     /locus_tag="QBA20_004524"
     CDS             210..>496
                     /locus_tag="QBA20_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6294751.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAG"
BASE COUNT          161 a           86 c           78 g          171 t
ORIGIN      
        1 tgaattaatg tattaaatta atattgtgaa tatttataaa ttcctaaaaa ataagtatcg
       61 tcattgtgaa agctactaaa tttcagtaag ttaattccca gcctttaaat ttaatgttaa
      121 ctaaaaatgc ttttttatat tgacttacaa taaatctaca tctctaatac accaactttt
      181 ttgaaacgat attgtaagat caaaattata tggtggcgaa aattaatctc ttattacggc
      241 ttacgccgct tttattcgtg ttcgctcctt ttttggcaca gtcaaatatg actgtttatc
      301 ctatgcctgt ttccattaat agtcagggtg aaggcaacgt tcgcgtaata tctaaaagta
      361 atgaagttca gtacatcaag gcgacagtat tccgtatcga taatccatcg acgcctcagg
      421 aaaatgaagt tgagattaaa tccggtgatg caaatcatct ggttgttatg ccacctaaat
      481 tcgctttacc agccgg
//
LOCUS       DAOFMX010000300          492 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.302, whole genome shotgun sequence.
ACCESSION   DAOFMX010000300 DAOFMX010000000
VERSION     DAOFMX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.302"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..491
                     /locus_tag="QBA20_004525"
     CDS             <1..491
                     /locus_tag="QBA20_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDQ6294752.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     EM"
BASE COUNT          111 a          107 c          125 g          149 t
ORIGIN      
        1 tactgggagc attgtgggtg cgcaatggca tggaggagtc cgcggaattt gaacaacagc
       61 aacataatca agcggccgcg aaaaaacgca tccctgttat cgaagctctg ttacgacatc
      121 ccggtgcttt cctgaagatt attgcactac gactgtgcga gttgctgacg atgtacattg
      181 ttactgcctt tgcacttaat tattcaaccc agaatatggg gttaccgcgc gaacttttcc
      241 ttaatattgg tttgctggta ggtggattaa gctgcctgac aattccctgt tttgcctggc
      301 ttgccgatcg ttttggtcgg cgcagggttt atatcacagg cgcgttgatc ggaacgttga
      361 gcgcatttcc tttctttatg gcgcttgaag cacaatctat tttctggata gttttcttct
      421 ccataatgct ggcaaacatt gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta
      481 ccgaaatgtg ag
//
LOCUS       DAOFMX010000301          470 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.303, whole genome shotgun sequence.
ACCESSION   DAOFMX010000301 DAOFMX010000000
VERSION     DAOFMX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.303"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            52..470
                     /locus_tag="QBA20_004526"
                     /pseudo
     CDS             52..470
                     /locus_tag="QBA20_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          153 a          116 c          109 g           92 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct
//
LOCUS       DAOFMX010000302          461 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.304, whole genome shotgun sequence.
ACCESSION   DAOFMX010000302 DAOFMX010000000
VERSION     DAOFMX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.304"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..461
                     /locus_tag="QBA20_004527"
                     /pseudo
     CDS             1..461
                     /locus_tag="QBA20_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          108 a          133 c          135 g           85 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc a
//
LOCUS       DAOFMX010000303          448 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.305, whole genome shotgun sequence.
ACCESSION   DAOFMX010000303 DAOFMX010000000
VERSION     DAOFMX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.305"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..375)
                     /locus_tag="QBA20_004528"
     CDS             complement(<1..375)
                     /locus_tag="QBA20_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294753.1"
                     /translation="MVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFGGM
                     VRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEFDI
                     WNLNETWAFQEKLIFCEDESSLL"
BASE COUNT          135 a          106 c           96 g          111 t
ORIGIN      
        1 taacagactt gattcatcct cacaaaatat aagtttttcc tggaatgccc atgtctcatt
       61 gaggttccag atatcaaatt cgaagtcgtg ataacgaaaa cggatcccac caaacttatt
      121 aaaacgcagt tgtttgacgg gcagttcagc caatgtctgg aagagttcct cacgggagcg
      181 accgatgacg atatcaaggt ctgaagagaa tccatcgacg ccatacaagc ctaagtcacg
      241 caccatcccg ccaaaaatcc tggccggaca ggaacaggcc agagtatcaa gtaatactct
      301 ggctttctta tgctcatcac taagcgtaaa aaaatcggcg atacgctgtt caagcagttg
      361 ggtgtttgag accacattca tacagtgatt ttagctcatc gtgaaggcgc attaaataac
      421 tatccgtcat gcctgagatt gcgtcggt
//
LOCUS       DAOFMX010000304          446 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.306, whole genome shotgun sequence.
ACCESSION   DAOFMX010000304 DAOFMX010000000
VERSION     DAOFMX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.306"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..395)
                     /locus_tag="QBA20_004529"
     CDS             complement(<1..395)
                     /locus_tag="QBA20_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDQ6294754.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGT"
BASE COUNT          105 a          123 c          123 g           95 t
ORIGIN      
        1 gtaccgctta ttgcgtgacc gatgacgtca atcatccatg ctgacaggtt ttgatgagca
       61 agttgctcga gtgattcgga tgtctggtca cgcagctggt tgtcgcagtg ccagcacaat
      121 accattgcgc cggtaccata acggtgaatg acggtttcgc tgtgatgata atcgccgtgt
      181 ggccactggc aggatttaat atggcgcaac agccagtcag acaatgcgcc agcaccacca
      241 gcagcacgaa tcacccgtgc gttactgaaa aacggcagca atgttttgtc ttccactagc
      301 ggctggcgaa cggcaggaac gaccccggac ggcagattac gcatgctttt cggttccggc
      361 tccaccagta cccgggtatt gtggaatacc ggcatggatt cacgaccggg cttaaggacc
      421 accagcccga gttccggaac cagaac
//
LOCUS       DAOFMX010000305          443 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.307, whole genome shotgun sequence.
ACCESSION   DAOFMX010000305 DAOFMX010000000
VERSION     DAOFMX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.307"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            213..>443
                     /locus_tag="QBA20_004530"
     CDS             213..>443
                     /locus_tag="QBA20_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDQ6294755.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          140 a          105 c           62 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatggcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAOFMX010000306          434 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.308, whole genome shotgun sequence.
ACCESSION   DAOFMX010000306 DAOFMX010000000
VERSION     DAOFMX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.308"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            35..434
                     /locus_tag="QBA20_004531"
                     /pseudo
     CDS             35..434
                     /locus_tag="QBA20_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAOFMX010000307          389 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.309, whole genome shotgun sequence.
ACCESSION   DAOFMX010000307 DAOFMX010000000
VERSION     DAOFMX010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.309"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(40..>389)
                     /locus_tag="QBA20_004532"
     CDS             complement(40..>389)
                     /locus_tag="QBA20_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDQ6294756.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           89 c           87 g          121 t
ORIGIN      
        1 ttcgtgaagc tgttgaaggg tggattctta acggtggcgt taatcagaaa tgctcctgat
       61 gtgccagaac aatttatcag cgtgaagctc atacgctttg cgctgagcct ctatccagtc
      121 gtgtttattg tatacggaca ttatcccgcc aagttcatga ccgagcattc gctcagtaac
      181 atgaggctcg acattcatct cggacagccg tgtaacaagc gttcttctga aatcatgtgt
      241 tcgccagtaa cctagatcca tccctcccct tattctattt acgaatcggt tggctgcagc
      301 gatgctgatc ggtttgttta tgtcttctcc agggaaaaga acatcattgt acattgtcat
      361 ggccttttcg agaaaaggct taatttgtt
//
LOCUS       DAOFMX010000308          385 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.310, whole genome shotgun sequence.
ACCESSION   DAOFMX010000308 DAOFMX010000000
VERSION     DAOFMX010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.310"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..>385)
                     /locus_tag="QBA20_004533"
     CDS             complement(<1..>385)
                     /locus_tag="QBA20_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6294757.1"
                     /translation="WNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGL
                     RYNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIIT
                     ETVRKTAMQITWRGMYQRLLKVDPL"
BASE COUNT           88 a          121 c           73 g          103 t
ORIGIN      
        1 ttcagcgggt caaccttcag taacctctga tacatccctc tccaggtgat ttgcatcgcc
       61 gtttttctca ctgtctccgt tatgatgtac accacttctt ccagtaaccg ccgtttcgcc
      121 ggactcaaaa aaccgtagta cctcaccata cggaacccct tatccgccac atgccaggag
      181 aacctttcca tgaactcatc tccactcatc aacaggtatt cttcccgttt tgttcggtga
      241 ctgttgtaac gcagaccgat ttcatcctga ccggcataat gctccagacg actcatcggc
      301 accggtggct ttttcaggta agagccaaag tacaccgcca catgggtggc attatccatt
      361 acccgggata cgttgacatt ccagc
//
LOCUS       DAOFMX010000309          380 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.311, whole genome shotgun sequence.
ACCESSION   DAOFMX010000309 DAOFMX010000000
VERSION     DAOFMX010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.311"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            11..>380
                     /locus_tag="QBA20_004534"
     CDS             11..>380
                     /locus_tag="QBA20_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDQ6294758.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNR"
BASE COUNT          110 a           87 c           94 g           89 t
ORIGIN      
        1 actaaaatct atggcgcttt ttcaacgagc aataaacaca caggcctatc taaaagcagg
       61 aattatgggg tttgctggcg acggcaaaac ctacacagca agcgaactgg ctattggtct
      121 ggtaatgctt atgcggcaac ggggaattga agctggtaac cgcccggtaa tgtttctgga
      181 cacagaaaca ggctctgact gggttaaacc ccgctttgat gcggaaaata tcgaattatt
      241 tacggcaaaa acccgctcgt ttgttgatct gcttgaagcc attaatgaag cggaaagcag
      301 cggttccgta atgatcatcg actccatcag tcacttctgg accggattat gcgacgagta
      361 tgccagacgc cgaaaccgta
//
LOCUS       DAOFMX010000310          375 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.312, whole genome shotgun sequence.
ACCESSION   DAOFMX010000310 DAOFMX010000000
VERSION     DAOFMX010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.312"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..>375
                     /locus_tag="QBA20_004535"
     CDS             <1..>375
                     /locus_tag="QBA20_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDQ6294759.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAOFMX010000311          375 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.313, whole genome shotgun sequence.
ACCESSION   DAOFMX010000311 DAOFMX010000000
VERSION     DAOFMX010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.313"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..>375
                     /locus_tag="QBA20_004536"
     CDS             <1..>375
                     /locus_tag="QBA20_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6294760.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGR
                     AFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          114 a           72 c          116 g           73 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggggaagggc cttcccgaga gtggtaaagg agaggccctg gaaatacccc acagccccga
      361 aaaagagcca gtcag
//
LOCUS       DAOFMX010000312          372 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.314, whole genome shotgun sequence.
ACCESSION   DAOFMX010000312 DAOFMX010000000
VERSION     DAOFMX010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.314"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(<1..311)
                     /locus_tag="QBA20_004537"
     CDS             complement(<1..311)
                     /locus_tag="QBA20_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDQ6294761.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAOFMX010000313          354 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.315, whole genome shotgun sequence.
ACCESSION   DAOFMX010000313 DAOFMX010000000
VERSION     DAOFMX010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.315"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            4..354
                     /locus_tag="QBA20_004538"
                     /pseudo
     CDS             4..354
                     /locus_tag="QBA20_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          112 a           78 c           86 g           78 t
ORIGIN      
        1 ataatgagca gaaaaaccca acgttactct acggagttta aagccgaagc cgtcaaaacg
       61 gttcctgaaa atcagctttc gatcagtgaa ggcgcttccc gattatctgt tcctgaaggc
      121 actttaggac aatgggttac cgccgcaaga aaagggctca atacaccagg ctcccgcaca
      181 gtggctgagc tggaatctga agttatgcaa ctgcgtaagg ctttaaatga agcacgactt
      241 gagcgagata tattaaaaaa agcaacagcg tattttgcac aggagtcgct gaaaaatacg
      301 cgttagtcga acaatggcga caacaattcc ctgttgaagc gatgtgtcgg gtac
//
LOCUS       DAOFMX010000314          349 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.316, whole genome shotgun sequence.
ACCESSION   DAOFMX010000314 DAOFMX010000000
VERSION     DAOFMX010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.316"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..347
                     /locus_tag="QBA20_004539"
     CDS             <1..347
                     /locus_tag="QBA20_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDQ6294762.1"
                     /translation="GGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQS
                     WLKENPKFRGIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVS
                     PFPGGKHGLAKV"
BASE COUNT          100 a           88 c           92 g           69 t
ORIGIN      
        1 tgggcggcaa cagcaaaagt tcggcgctgt tcatcagcct gctgaagcgg cttaaagcga
       61 cataccgtcg tgcgaaaacc atcacgctga tcgtggacaa ctacattatc cacaaaagcc
      121 gggaaacaca gagctggctg aaggagaacc cgaagttcag gggcatttat cagccggttt
      181 actcgccatg ggtgaatcat gttgaacggc tatggcaggc acttcacgac acaataacgc
      241 gtaatcatca gtgccgctca atgtggcaac tgttgaaaaa agttcgccat tttatggaaa
      301 ccgtcagccc attccccgga ggcaaacatg ggctggcaaa agtgtagcg
//
LOCUS       DAOFMX010000315          340 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.317, whole genome shotgun sequence.
ACCESSION   DAOFMX010000315 DAOFMX010000000
VERSION     DAOFMX010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.317"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            3..170
                     /gene="xisR"
                     /locus_tag="QBA20_004540"
     CDS             3..170
                     /gene="xisR"
                     /locus_tag="QBA20_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HDQ6294763.1"
                     /translation="MLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYN
                     YPKINQFIKDS"
     gene            172..>340
                     /locus_tag="QBA20_004541"
     CDS             172..>340
                     /locus_tag="QBA20_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HDQ6294764.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           63 c           83 g           86 t
ORIGIN      
        1 tgatgcttcg cactggtctg ggtgccagac aaattgaagc ataccgccag aactgttggg
       61 tggaaggctt ccacttcaaa cgagtatctc ctttagggaa gccagacagt aagcgaggga
      121 ttatctggta caactatccg aagataaatc agtttatcaa agactcatga tatgtctaaa
      181 ttaccaacag gtgtcgagat tcgaggtaaa tacattcgca tctggttcat gtttcgagga
      241 aaacgatgtc gggaaacatt gaaaggctgg gagattacta acagtaacat taaaaaggcc
      301 gggaatttaa gagcgttgat agttcatcaa atcagttccg
//
LOCUS       DAOFMX010000316          338 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.318, whole genome shotgun sequence.
ACCESSION   DAOFMX010000316 DAOFMX010000000
VERSION     DAOFMX010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.318"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            <1..>338
                     /locus_tag="QBA20_004542"
     CDS             <1..>338
                     /locus_tag="QBA20_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDQ6294765.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLAD"
BASE COUNT           93 a           79 c           78 g           88 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcggatt
//
LOCUS       DAOFMX010000317          327 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.denovo.319, whole genome shotgun sequence.
ACCESSION   DAOFMX010000317 DAOFMX010000000
VERSION     DAOFMX010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.denovo.319"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            1..327
                     /locus_tag="QBA20_004543"
                     /pseudo
     CDS             1..327
                     /locus_tag="QBA20_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           82 a           79 c          101 g           65 t
ORIGIN      
        1 acgtattacc actgtcagca acagcgacat catccggata aacgcagtgc ccgtgcgcag
       61 cgcgatgact ggctgaagaa agagatacag cgcgtatacg atgaaaatca caaggtatac
      121 ggtgtaaagt ctggcgtcag ttgttacggg aaggtatcag agtggccaga tgcactgtgg
      181 cacgtctcat ggcggttatg ggacttgccg gtgttctccg gggtaaaaag gtccgtacga
      241 ccatcagccg gaaagccgtt gccgcaggcg accgcgtaaa ccgtcagttc gtggcagaac
      301 gacctgacca gctgtgggtg gctgatt
//
LOCUS       DAOFMX010000318         9722 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.guided.126, whole genome shotgun sequence.
ACCESSION   DAOFMX010000318 DAOFMX010000000
VERSION     DAOFMX010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.guided.126"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            complement(17..205)
                     /locus_tag="QBA20_004544"
     CDS             complement(17..205)
                     /locus_tag="QBA20_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDQ6294766.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(424..1401)
                     /gene="epmA"
                     /locus_tag="QBA20_004545"
     CDS             complement(424..1401)
                     /gene="epmA"
                     /locus_tag="QBA20_004545"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDQ6294767.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1726..3534
                     /gene="frdA"
                     /locus_tag="QBA20_004546"
     CDS             1726..3534
                     /gene="frdA"
                     /locus_tag="QBA20_004546"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDQ6294768.1"
                     /translation="MQTFQADLAIVGAGGSGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3527..4261
                     /gene="frdB"
                     /locus_tag="QBA20_004547"
     CDS             3527..4261
                     /gene="frdB"
                     /locus_tag="QBA20_004547"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDQ6294769.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4272..4667
                     /gene="frdC"
                     /locus_tag="QBA20_004548"
     CDS             4272..4667
                     /gene="frdC"
                     /locus_tag="QBA20_004548"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDQ6294770.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4678..5037
                     /gene="frdD"
                     /locus_tag="QBA20_004549"
     CDS             4678..5037
                     /gene="frdD"
                     /locus_tag="QBA20_004549"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDQ6294771.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5100..6233
                     /locus_tag="QBA20_004550"
     CDS             5100..6233
                     /locus_tag="QBA20_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDQ6294772.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6322..6855
                     /gene="blc"
                     /locus_tag="QBA20_004551"
     CDS             6322..6855
                     /gene="blc"
                     /locus_tag="QBA20_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDQ6294773.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6852..7169)
                     /gene="sugE"
                     /locus_tag="QBA20_004552"
     CDS             complement(6852..7169)
                     /gene="sugE"
                     /locus_tag="QBA20_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDQ6294774.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7345..7491)
                     /gene="ecnB"
                     /locus_tag="QBA20_004553"
     CDS             complement(7345..7491)
                     /gene="ecnB"
                     /locus_tag="QBA20_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDQ6294775.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7602..7727)
                     /gene="ecnA"
                     /locus_tag="QBA20_004554"
     CDS             complement(7602..7727)
                     /gene="ecnA"
                     /locus_tag="QBA20_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDQ6294776.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7779..8345)
                     /gene="efp"
                     /locus_tag="QBA20_004555"
     CDS             complement(7779..8345)
                     /gene="efp"
                     /locus_tag="QBA20_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDQ6294777.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8387..9415
                     /gene="epmB"
                     /locus_tag="QBA20_004556"
     CDS             8387..9415
                     /gene="epmB"
                     /locus_tag="QBA20_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDQ6294778.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2475 a         2485 c         2472 g         2290 t
ORIGIN      
        1 tgaacataaa acactatcag taagttggag tcattaccat taaggcgaat ggaataaaga
       61 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
      121 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
      181 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
      241 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
      301 atacttggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
      361 catatgctgc agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      421 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
      481 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      541 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      601 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc
      661 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
      721 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
      781 ttctttacca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
      841 gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgcgactt cccgcagttg
      901 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
      961 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     1021 caaccggtac atatcatagt gcggtcgata ccactccagc atagtgaact cagggttgtg
     1081 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     1141 agcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     1201 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1261 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
     1321 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     1381 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1441 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     1501 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1561 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1621 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     1681 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     1741 gccgatcttg ccattgtagg cgccggtggc tcgggattac gtgctgcaat tgctgccgcg
     1801 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     1861 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     1921 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     1981 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtggggatg cccatggagc
     2041 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     2101 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     2161 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     2221 ggtcatgttc gcggcctggt agcaataaac atgatggaag gcacgttggt gcagatccgt
     2281 gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg tttatcgtta caacaccaac
     2341 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2401 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
     2461 gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2521 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2581 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     2641 tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc acctcggcga gaaaaaactg
     2701 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     2761 aaagaaccga ttccggtacg tccgactgca cactacacca tgggcggtat cgaaaccgat
     2821 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     2881 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     2941 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
     3001 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctagttaa ccaggatggc
     3061 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga aggttgcggt
     3121 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     3181 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
     3241 accattgaac tgagccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3301 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
     3361 gtcaactttc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
     3421 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
     3481 gatgcagccg ataaggcgga agcagccaat aataaggaga aggcgaatgg ctgagatgaa
     3541 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3601 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     3661 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
     3721 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     3781 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
     3841 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     3901 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     3961 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     4021 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     4081 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggtg tatggagctg
     4141 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     4201 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4261 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4321 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4381 tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc ccggaagcct
     4441 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     4501 tgacggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4561 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4621 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     4681 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     4741 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     4801 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     4861 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     4921 cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc ctgcgggcaa atgggttttc
     4981 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac aatctaacgc
     5041 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     5101 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttactccaca tttgctgccc
     5161 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     5221 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     5281 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5341 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     5401 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     5461 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg ccggatgaag
     5521 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5581 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     5641 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     5701 accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg ggatatcgcg
     5761 aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     5821 cgaccattga agatatggcc cgctgggtgc aaagcaattt accccccctt gatatcaatg
     5881 agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
     5941 tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta
     6001 acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa
     6061 ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct
     6121 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
     6181 ccaatccagc gagagtcgcc gccgcctgtc agattctcaa cgctctacag taaaatttca
     6241 tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa
     6301 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
     6361 tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg gcgtgaccgt agtgaataat
     6421 ttcgacgcca aacgttatct cggtacctgg tatgagattg cccgttttga tcaccgtttt
     6481 gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat
     6541 gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga agggaaagcg
     6601 tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat
     6661 ggcggttata acgttattgc actcgatcgg gaataccgcc atgcgctggt ttgcggcccg
     6721 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
     6781 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
     6841 cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
     6901 agactcgcca gacgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc
     6961 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctaccgg taacgatttc
     7021 atcgcccagg caagtagcgc catactgact atcatcgccg tcacggtaat aacactcggc
     7081 gtcaaacggc taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga
     7141 ccagcaataa ctaagataat ccaggacata tcaggctccg gaaatttggg gccgtccccg
     7201 gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
     7261 ggtggcaggg gaatttcaac tattaccttt atgacgatcg cagaaaaaac aaacggcacg
     7321 acactgctgt cgtaccgtta ttgcttattg ctgcgctttc gttgctgcgc cagaaatcgc
     7381 gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg cagttaatac
     7441 tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc
     7501 cttttaggta atgttattta tgtttgccta tagcaaatta ataatagaca acatcgccaa
     7561 aaatgatgga aatctgatga tttttcggaa gagaagaaaa attagctggc agcgcgagag
     7621 atggagttac cgagatgttt gatgtcttcg ccgaaaccgc aagcggtgtt acagcccgtg
     7681 agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc tgacctgaat
     7741 aagtgatggt gcagcctgca ggccgcacca caaccgcatt acttcacgcg agagacgtat
     7801 tcaccagagc gggtatccac tttgatgact tcgccgattt gtacgaacag cggaacttta
     7861 accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt atcacctttc
     7921 aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt aacggagatc
     7981 ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcagcca tttagcattg
     8041 tcgccaattg cttttgcatc agcagacagc tgctcgaaag tttcgttgtt catgaagtgc
     8101 cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc agcgccttca
     8161 gcggaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag acgacgcagt
     8221 ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc ttcaaccgcg
     8281 taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct atagtacgtt
     8341 gccataaggc cctctgaaat ttgttaattg gtagctaagc cacaaaatgg cgcatattgt
     8401 aaccctaaat accccatcca gagaagattg gttaacgcaa cttgccgatg ttgtgaccga
     8461 tcctgatgaa cttctgcgtc ttttgaatat agacgcggac gaaaaactgt tggccggacg
     8521 cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca tttatcgatc gcatggagaa
     8581 aggcaatccg gacgatcctc ttttgcgtca ggtacttacc tcgcaagatg agtttgtcgt
     8641 cgcgtccgga ttctccaccg acccactgga agaacagcac agcgtagtgc ctggtttgtt
     8701 gcataaatac cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg
     8761 ctattgcttc cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc gtaactggca
     8821 gactgcactt gagtatgttg ctgcgcatcc ggaactggac gagatgattt tctccggcgg
     8881 cgatccgctg atggcgaaag agcacgagct ggactggttg ctcacacaac tggaagccat
     8941 cccgcatatc aaacgtctgc ggattcacag ccgtctgccg attgtgatcc cggcacgtat
     9001 caccgaggcg ctggttgaac gctttgcccg ttctacgctg caaatcttgc tggtgaatca
     9061 catcaatcat gccaatgagg tagatgaaac gttccgtcag gcgatggcta aattgcgccg
     9121 tgtcggtgtc accctgctta accagagcgt tctgttacgt ggtgtgaacg ataacgcaca
     9181 aacgttggca aacctgagta atgcgttgtt cgatgccggc gtgatgccgt attacctgca
     9241 tgtgctagat aaagtacagg gcgcggcgca ttttatggtg agtgatgacg aagcacggca
     9301 gattatgcgt gagttgctga cattggtgtc gggatatctg gtgccgaaac tggcgcgaga
     9361 aattggcggc gaacccagca aaacgccgct ggatctccag ctacgccagc agtaactgat
     9421 taaacaggga agttaaacat taaaattgcc tgatgcgcta cgcttatcag gcctacatcg
     9481 tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg gataaggcgt ttacgccgca
     9541 tccggcaaga gcaacaaacg caagtacagc aacctgtcat taaactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt tcttttcggt aatgactcca acttactgat agtgttttat
     9721 gt
//
LOCUS       DAOFMX010000319         7053 bp    DNA     linear   BCT 06-APR-2023
DEFINITION  TPA_asm: Shigella flexneri strain Sbovis_F20.fasta
            SAMN27009882-rid17588133.guided.152, whole genome shotgun sequence.
ACCESSION   DAOFMX010000319 DAOFMX010000000
VERSION     DAOFMX010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN27009882
            Sequence Read Archive: SRR18753310
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001680749.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001680749.1
            Genome Coverage       :: 223x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/06/2023 20:54:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,563
            CDSs (total)                      :: 4,474
            Genes (coding)                    :: 4,077
            CDSs (with protein)               :: 4,077
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 76
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 180 of 397
            Pseudo Genes (incomplete)         :: 105 of 397
            Pseudo Genes (internal stop)      :: 155 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN27009882-rid17588133.guided.152"
                     /strain="Sbovis_F20.fasta"
                     /host="yak"
                     /db_xref="taxon:623"
                     /country="China: Gansu"
                     /collection_date="2017"
     gene            59..1102
                     /locus_tag="QBA20_004557"
     CDS             59..1102
                     /locus_tag="QBA20_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDQ6294779.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            1164..1778
                     /locus_tag="QBA20_004558"
                     /pseudo
     CDS             1164..1778
                     /locus_tag="QBA20_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="QBA20_004559"
     CDS             complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="QBA20_004559"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDQ6294780.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3554..4012
                     /gene="gpt"
                     /locus_tag="QBA20_004560"
     CDS             3554..4012
                     /gene="gpt"
                     /locus_tag="QBA20_004560"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDQ6294781.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4104..5348
                     /gene="frsA"
                     /locus_tag="QBA20_004561"
     CDS             4104..5348
                     /gene="frsA"
                     /locus_tag="QBA20_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDQ6294782.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5406..5807
                     /gene="crl"
                     /locus_tag="QBA20_004562"
     CDS             5406..5807
                     /gene="crl"
                     /locus_tag="QBA20_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDQ6294783.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="QBA20_004563"
     CDS             complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="QBA20_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDQ6294784.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1715 a         1735 c         1816 g         1787 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
      901 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      961 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
     1021 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
     1081 atgtctgggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
     1141 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
     1201 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
     1261 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
     1321 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
     1381 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
     1441 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
     1501 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
     1561 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
     1621 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
     1681 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
     1741 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
     1801 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1861 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1921 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1981 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     2041 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     2101 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     2161 tgctcaccac gtagtcttta ccgctgtcga tcagtgaacg gatcaggcag tgaatttcta
     2221 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     2281 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     2341 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     2401 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     2461 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     2521 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     2581 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     2641 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     2701 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     2761 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     2821 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2881 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2941 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     3001 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     3061 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggtttac
     3121 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     3181 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     3241 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     3301 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     3361 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     3421 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3481 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3541 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3601 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3661 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     3781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3841 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     4021 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     4081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     4141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     4201 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     4261 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     4321 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     4381 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4441 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4501 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4561 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4621 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4681 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4741 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     4801 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4861 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4921 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4981 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     5041 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     5101 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     5161 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     5221 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     5281 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     5341 tgtgttaaaa atttgctaaa ttttgctaat ttggtaaaac agttgcatca caacaggaga
     5401 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5461 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5521 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5581 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5641 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5701 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5761 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5821 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
     5881 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
     5941 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
     6001 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     6061 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     6121 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
     6181 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     6241 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     6301 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     6361 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     6421 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     6481 cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt
     6541 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     6601 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctccgcttta tttccggcaa
     6661 actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgagtt tcacctttaa
     6721 aaccaaaacg gatataactc tggtcgccat ctttactggc gttatcactc atataatgca
     6781 tggctttaac tttgccatag acatccagtt tattaccgtc tttattatat atttctgcag
     6841 cctgtacaga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
     6901 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
     6961 ggaaagtttt taacctgaga ttgttaaaga tatattacag attaatgata ttctgaaaat
     7021 gggtaatgct gccaacttac tgatttagtg tat
//
